LOCUS       DAZOYS010000001        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOYS010000001 DAZOYS010000000
VERSION     DAZOYS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.001"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUN9_000001"
     CDS             <1..468
                     /locus_tag="ACHUN9_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0693530.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUN9_000002"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUN9_000002"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0693531.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUN9_000003"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUN9_000003"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0693532.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUN9_000004"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUN9_000004"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0693533.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUN9_000005"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUN9_000005"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0693534.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUN9_000006"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUN9_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0693535.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUN9_000007"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUN9_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0693536.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUN9_000008"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUN9_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0693537.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUN9_000009"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUN9_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0693538.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUN9_000010"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUN9_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0693539.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUN9_000011"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUN9_000011"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0693540.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUN9_000012"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUN9_000012"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0693541.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUN9_000013"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUN9_000013"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0693542.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUN9_000014"
     CDS             13322..13669
                     /locus_tag="ACHUN9_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0693543.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUN9_000015"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUN9_000015"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0693544.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUN9_000016"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUN9_000016"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0693545.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUN9_000017"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUN9_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0693546.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUN9_000018"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUN9_000018"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0693547.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHUN9_000019"
     CDS             18978..20735
                     /locus_tag="ACHUN9_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0693548.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUN9_000020"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUN9_000020"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0693549.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUN9_000021"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUN9_000021"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0693550.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUN9_000022"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUN9_000022"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0693551.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUN9_000023"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUN9_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0693552.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUN9_000024"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUN9_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0693553.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUN9_000025"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUN9_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0693554.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUN9_000026"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUN9_000026"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0693555.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUN9_000027"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUN9_000027"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0693556.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUN9_000028"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUN9_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0693557.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUN9_000029"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUN9_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR0693558.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUN9_000030"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUN9_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR0693559.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUN9_000031"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUN9_000031"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0693560.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHUN9_000032"
     CDS             complement(36854..36898)
                     /locus_tag="ACHUN9_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693561.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUN9_000033"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUN9_000033"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0693562.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUN9_000034"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUN9_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0693563.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUN9_000035"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUN9_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0693564.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUN9_000036"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUN9_000036"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0693565.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUN9_000037"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUN9_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0693566.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUN9_000038"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUN9_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0693567.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUN9_000039"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUN9_000039"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0693568.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUN9_000040"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUN9_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0693569.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUN9_000041"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUN9_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0693570.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUN9_000042"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUN9_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0693571.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUN9_000043"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUN9_000043"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0693572.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUN9_000044"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUN9_000044"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0693573.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUN9_000045"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUN9_000045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0693574.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUN9_000046"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUN9_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0693575.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUN9_000047"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUN9_000047"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0693576.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUN9_000048"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUN9_000048"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0693577.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUN9_000049"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUN9_000049"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0693578.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUN9_000050"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUN9_000050"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0693579.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUN9_000051"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUN9_000051"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0693580.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUN9_000052"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUN9_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0693581.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUN9_000053"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUN9_000053"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0693582.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUN9_000054"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUN9_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0693583.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16851 c        15001 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZOYS010000002        59056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOYS010000002 DAZOYS010000000
VERSION     DAZOYS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.002"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(88..1035)
                     /gene="dusC"
                     /locus_tag="ACHUN9_000055"
     CDS             complement(88..1035)
                     /gene="dusC"
                     /locus_tag="ACHUN9_000055"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0693584.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1274..1672
                     /locus_tag="ACHUN9_000056"
     CDS             1274..1672
                     /locus_tag="ACHUN9_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0693585.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1669..2364
                     /locus_tag="ACHUN9_000057"
     CDS             1669..2364
                     /locus_tag="ACHUN9_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0693586.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2494..3378
                     /gene="cdd"
                     /locus_tag="ACHUN9_000058"
     CDS             2494..3378
                     /gene="cdd"
                     /locus_tag="ACHUN9_000058"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0693587.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3528..4247
                     /gene="sanA"
                     /locus_tag="ACHUN9_000059"
     CDS             3528..4247
                     /gene="sanA"
                     /locus_tag="ACHUN9_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0693588.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4250..4489
                     /locus_tag="ACHUN9_000060"
     CDS             4250..4489
                     /locus_tag="ACHUN9_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0693589.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4683..5921
                     /gene="preT"
                     /locus_tag="ACHUN9_000061"
                     /pseudo
     CDS             4683..5921
                     /gene="preT"
                     /locus_tag="ACHUN9_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5915..7150
                     /gene="preA"
                     /locus_tag="ACHUN9_000062"
     CDS             5915..7150
                     /gene="preA"
                     /locus_tag="ACHUN9_000062"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0693590.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7277..8287)
                     /gene="mglC"
                     /locus_tag="ACHUN9_000063"
     CDS             complement(7277..8287)
                     /gene="mglC"
                     /locus_tag="ACHUN9_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0693591.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8303..9823)
                     /gene="mglA"
                     /locus_tag="ACHUN9_000064"
     CDS             complement(8303..9823)
                     /gene="mglA"
                     /locus_tag="ACHUN9_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0693592.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9884..10882)
                     /gene="mglB"
                     /locus_tag="ACHUN9_000065"
     CDS             complement(9884..10882)
                     /gene="mglB"
                     /locus_tag="ACHUN9_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0693593.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11162..12160)
                     /gene="galS"
                     /locus_tag="ACHUN9_000066"
     CDS             complement(11162..12160)
                     /gene="galS"
                     /locus_tag="ACHUN9_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0693594.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12344..13501)
                     /gene="yeiB"
                     /locus_tag="ACHUN9_000067"
     CDS             complement(12344..13501)
                     /gene="yeiB"
                     /locus_tag="ACHUN9_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0693595.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13518..14186)
                     /gene="folE"
                     /locus_tag="ACHUN9_000068"
     CDS             complement(13518..14186)
                     /gene="folE"
                     /locus_tag="ACHUN9_000068"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0693596.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14444..15280
                     /gene="yeiG"
                     /locus_tag="ACHUN9_000069"
     CDS             14444..15280
                     /gene="yeiG"
                     /locus_tag="ACHUN9_000069"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0693597.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15312..17303)
                     /gene="cirA"
                     /locus_tag="ACHUN9_000070"
     CDS             complement(15312..17303)
                     /gene="cirA"
                     /locus_tag="ACHUN9_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0693598.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17595..19064)
                     /gene="lysP"
                     /locus_tag="ACHUN9_000071"
     CDS             complement(17595..19064)
                     /gene="lysP"
                     /locus_tag="ACHUN9_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0693599.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19269..20150)
                     /gene="yieE"
                     /locus_tag="ACHUN9_000072"
     CDS             complement(19269..20150)
                     /gene="yieE"
                     /locus_tag="ACHUN9_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0693600.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20249..21298
                     /locus_tag="ACHUN9_000073"
     CDS             20249..21298
                     /locus_tag="ACHUN9_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0693601.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21372..22229
                     /gene="nfo"
                     /locus_tag="ACHUN9_000074"
     CDS             21372..22229
                     /gene="nfo"
                     /locus_tag="ACHUN9_000074"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0693602.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22233..23321
                     /locus_tag="ACHUN9_000075"
     CDS             22233..23321
                     /locus_tag="ACHUN9_000075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0693603.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23377..24627)
                     /locus_tag="ACHUN9_000076"
     CDS             complement(23377..24627)
                     /locus_tag="ACHUN9_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0693604.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24727..25668)
                     /gene="rihB"
                     /locus_tag="ACHUN9_000077"
     CDS             complement(24727..25668)
                     /gene="rihB"
                     /locus_tag="ACHUN9_000077"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0693605.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25798..26496
                     /locus_tag="ACHUN9_000078"
     CDS             25798..26496
                     /locus_tag="ACHUN9_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0693606.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26563..27813)
                     /locus_tag="ACHUN9_000079"
     CDS             complement(26563..27813)
                     /locus_tag="ACHUN9_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0693607.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27907..28845)
                     /gene="psuG"
                     /locus_tag="ACHUN9_000080"
     CDS             complement(27907..28845)
                     /gene="psuG"
                     /locus_tag="ACHUN9_000080"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0693608.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28833..29774)
                     /locus_tag="ACHUN9_000081"
     CDS             complement(28833..29774)
                     /locus_tag="ACHUN9_000081"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0693609.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30232..31923)
                     /gene="fruA"
                     /locus_tag="ACHUN9_000082"
     CDS             complement(30232..31923)
                     /gene="fruA"
                     /locus_tag="ACHUN9_000082"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0693610.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31940..32878)
                     /gene="fruK"
                     /locus_tag="ACHUN9_000083"
     CDS             complement(31940..32878)
                     /gene="fruK"
                     /locus_tag="ACHUN9_000083"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0693611.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32878..34008)
                     /gene="fruB"
                     /locus_tag="ACHUN9_000084"
     CDS             complement(32878..34008)
                     /gene="fruB"
                     /locus_tag="ACHUN9_000084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0693612.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34376..35557
                     /gene="setB"
                     /locus_tag="ACHUN9_000085"
     CDS             34376..35557
                     /gene="setB"
                     /locus_tag="ACHUN9_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0693613.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35554..35808)
                     /locus_tag="ACHUN9_000086"
     CDS             complement(35554..35808)
                     /locus_tag="ACHUN9_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0693614.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35963..36535
                     /gene="yeiP"
                     /locus_tag="ACHUN9_000087"
     CDS             35963..36535
                     /gene="yeiP"
                     /locus_tag="ACHUN9_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0693615.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36758..38224
                     /locus_tag="ACHUN9_000088"
     CDS             36758..38224
                     /locus_tag="ACHUN9_000088"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0693616.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38342..39328
                     /gene="yeiR"
                     /locus_tag="ACHUN9_000089"
     CDS             38342..39328
                     /gene="yeiR"
                     /locus_tag="ACHUN9_000089"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0693617.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39367..40080
                     /gene="lpxT"
                     /locus_tag="ACHUN9_000090"
     CDS             39367..40080
                     /gene="lpxT"
                     /locus_tag="ACHUN9_000090"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0693618.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40492..41058
                     /gene="mepS"
                     /locus_tag="ACHUN9_000091"
     CDS             40492..41058
                     /gene="mepS"
                     /locus_tag="ACHUN9_000091"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0693619.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41239..42795
                     /locus_tag="ACHUN9_000092"
     CDS             41239..42795
                     /locus_tag="ACHUN9_000092"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0693620.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42877..44691
                     /locus_tag="ACHUN9_000093"
     CDS             42877..44691
                     /locus_tag="ACHUN9_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0693621.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44692..45786
                     /locus_tag="ACHUN9_000094"
     CDS             44692..45786
                     /locus_tag="ACHUN9_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0693622.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45786..46811
                     /locus_tag="ACHUN9_000095"
     CDS             45786..46811
                     /locus_tag="ACHUN9_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0693623.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46813..48402
                     /gene="yejF"
                     /locus_tag="ACHUN9_000096"
     CDS             46813..48402
                     /gene="yejF"
                     /locus_tag="ACHUN9_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0693624.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVHALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48406..48750)
                     /locus_tag="ACHUN9_000097"
     CDS             complement(48406..48750)
                     /locus_tag="ACHUN9_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0693625.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49083..50140)
                     /locus_tag="ACHUN9_000098"
                     /pseudo
     CDS             complement(49083..50140)
                     /locus_tag="ACHUN9_000098"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50231..50926)
                     /gene="rsuA"
                     /locus_tag="ACHUN9_000099"
     CDS             complement(50231..50926)
                     /gene="rsuA"
                     /locus_tag="ACHUN9_000099"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0693626.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51076..52836
                     /locus_tag="ACHUN9_000100"
     CDS             51076..52836
                     /locus_tag="ACHUN9_000100"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0693627.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52961..53245
                     /gene="rplY"
                     /locus_tag="ACHUN9_000101"
     CDS             52961..53245
                     /gene="rplY"
                     /locus_tag="ACHUN9_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0693628.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53384..54391)
                     /gene="yejK"
                     /locus_tag="ACHUN9_000102"
     CDS             complement(53384..54391)
                     /gene="yejK"
                     /locus_tag="ACHUN9_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0693629.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54573..54800
                     /locus_tag="ACHUN9_000103"
     CDS             54573..54800
                     /locus_tag="ACHUN9_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0693630.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54820..56580
                     /gene="yejM"
                     /locus_tag="ACHUN9_000104"
     CDS             54820..56580
                     /gene="yejM"
                     /locus_tag="ACHUN9_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0693631.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56655..56731
                     /locus_tag="ACHUN9_000105"
     tRNA            56655..56731
                     /locus_tag="ACHUN9_000105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56689..56691,aa:Pro,seq:ggg)
     gene            complement(56834..59056)
                     /gene="yejO"
                     /locus_tag="ACHUN9_000106"
                     /pseudo
     CDS             complement(56834..59056)
                     /gene="yejO"
                     /locus_tag="ACHUN9_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14540 a        15410 c        14404 g        14702 t
ORIGIN      
        1 cgcctcggca attttgacgc caccaaacaa ccgtgcggcc tattgataaa gagctaacac
       61 attgtcaaaa aacatcacta tggtttttta gagtttctcg atatcaattg cctgaatagc
      121 ccttgcaata tcaggggaat tattcaacac ccgaacatgc tgaaataatt ccgttgcttc
      181 atcgtattct ttacgcaaat aactcaacca ctgtttaatc cgcgcaacgt gatataaccc
      241 ggtatcgccc tgcttttcca gacgggtata tttttgcagc aaagcaacca cctccggcca
      301 cggcattcgc ggttcgttat attttactac ccggctcagg ttgggaatat tgagcgcccc
      361 gcgaccaatc atcactgcgt cgcagccgct gatcgcaatg cattgttgcg cgctctgcca
      421 gtcccagatt tcgccgttgg caatcaccgg aatattcagc cgctggcgaa tctcgccaat
      481 cgcctgccag tcaatatgct ccgcgcggta accctgctct tttgtccgtc catgcaccac
      541 cagctccgta gcaccagcct gttgaaccgc atcggcgatt tcaaatttct tctcaccgct
      601 gtcccagccc agacgcactt tcacgctgac gggcaaatgc gccggtacag cttcacgcat
      661 cgcttttgca ccctggtaga tgagttcagg atctttgagt aacgtcgccc cgccgccgct
      721 gccgttaacc gctttcgacg ggcagccgca attgagatcc acgccccagg aacctaattc
      781 caccgcacgg gcggcgttct ctgccagcca ttgtgggaac tgacctaaca actgaacgcg
      841 caccagcgta ccggatggtg tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa
      901 gacttttacc ggcagcagtt gatccaccac gcggacaaac tcggtgatgc acagatcgta
      961 gtcgttaact tcggtcagca attctcgcac cagagagtca agcacaccct ccatcggagc
     1021 cagtaacaca cgcatatctt cacccggaaa aaaatgaggc gctatgttag cgcctatggt
     1081 caacggatta aaggtctgca aagagggatt gctgcaaact gtaatccctc tctatgcttg
     1141 atgtatgcct gagttcgttg cgcattgcgt aatgccgggg agcgaaaaaa taagcgctga
     1201 tttcttaatg tgatcggtag cacgttttac gaattaattg tatgatgaat ccatctcatc
     1261 tggggtgttg attatgagta agacactgaa cattatctgg caatatttac gcgctttcgt
     1321 cctgatttat gcctgcctgt atgcaggcat tttcattgct tccctgctac cggtaaccat
     1381 tccgggcagc atcatcggga tgctgatcct gtttgtcctg ctggccttgc aaattcttcc
     1441 ggcaaaatgg gtcaatccgg ggtgctacgt actgattcgc tatatggcgc tattgtttgt
     1501 gccgattggc gtaggcgtca tgcaatattt tgatttgctc cgcgcacagt ttggcccggt
     1561 agtggtttcc tgtgcagtca gtacgctggt ggtttttctg gtggtgagct ggagttcaca
     1621 actggtacac ggtgaacgta aagtcgtagg tcagaaagga tcagaagaat gatggcgaat
     1681 atctggtggt cattaccgct gacgttgatt gtcttttttg ccgcccgcaa actggcggca
     1741 cggtataagt ttcccctgct taacccgttg ctggtagcaa tggtggtgat cattcctttt
     1801 ttaatgctga ccggcatctc ttacgacagc tactttaaag gtagcgaagt gctaaacgat
     1861 ctgctgcaac cggcggtcgt cgcgctggcc tatcctttat atgagcagct acaccagatc
     1921 cgcgcgcgct ggaaatcgat catcaccatc tgttttatcg gcagcgtggt tgcgatggtg
     1981 acggggactt ccgtggcatt gttgatgggc gcttcaccgg aaatcgccgc gtcaatcctg
     2041 ccgaaatcag tcaccacgcc tattgcaatg gcggttggcg gcagtattgg cggtattccg
     2101 gcaatcagcg cagtttgcgt gattttcgtc ggcatcctcg gcgcggtatt tggccataca
     2161 ttgcttaatg cgatgcgtat tcgtaccaaa gctgcgcgcg gtctggcaat ggggacagcc
     2221 tcgcacgccc tcggtacggc gcgctgcgcc gagctggatt atcaggaagg tgcatttagt
     2281 tcgctggcgc tggtgttatg cgggataatt acttcgctga tcgcaccgtt ccttttcccg
     2341 attattctgg cagtaatggg ctaaaatttg cgatgcgtcg cgcatttttg atgtaagttt
     2401 cacgcgttgc ataattaatg agattcagat cacatataaa gccataacgg gttcgtaaac
     2461 tgttatccca ttacatgatt atgaggcaac gccatgcatc cacgttttca aaccgctttt
     2521 gcccaacttg cggataactt gcaatctgca ctggaaccta ttctggcaga caagtacttc
     2581 cccgctttgt tgaccgggga gcaagtctca tcgctgaaga gcgcaacggg gctggacgaa
     2641 gacgcgctgg cattcgcact acttccactg gcggcggcct gtgcgcgtac gccattgtcg
     2701 aattttaatg ttggcgcaat tgcgcgcggt gtgagcggaa cctggtattt cggtgccaat
     2761 atggaattta ttggtgcaac aatgcagcaa accgttcatg ccgaacaaag cgcgatcagc
     2821 cacgcctggt tgagtggtga aaaagcgctt gcagccatca ccgttaacta tacgccttgt
     2881 ggtcactgcc gtcagtttat gaatgaactg aacagcggtc tggatctgcg tattcatctg
     2941 ccgggccgcg aggcacacgc gctgcgtgac tatctgccgg atgcctttgg gccgaaagat
     3001 ctggagatta aaacgctgct gatggacgaa caggatcacg gctatgcgct gacgggtgat
     3061 gcgctttctc aggcagcgat tgcggcggca aaccgttcgc acatgcctta cagtaagtcg
     3121 ccaagcggtg tcgcgctgga atgtaaagac ggtcgtattt tcagcggcag ctacgctgaa
     3181 aacgccgcat tcaacccgac tctgccaccg ttgcaggggg cgttaattct gttgaatctc
     3241 aagggttatg attacccgga tatccagcgc gcggttctgg cagaaaaagc cgatgcgccg
     3301 ttgattcagt gggatgccac ctccgcaacg ctgaaagctc tcggctgtca cagtatcgac
     3361 cgcgtgcttc tcgcttaagc ctggtgccgg atgcggcgtg aacgccttgt ccggcttgcc
     3421 agccctctcc tggtgtcgaa attcccggca aacagtttgc cgtttcttgc gcaaaaccag
     3481 cgggtaaagt agcctgatgg aaattttcct tagatcgagt ctcctgcatg ttaaagcgcg
     3541 tgttcctcag cctgttagtc ctgatcggct tgctgctgtt gactgtgctc ggcctcgatc
     3601 gctggatgag ctggaaaacc gcgccttata tctacgacga attgcaggat ctcccctacc
     3661 gccaggtcgg cgtggtgctc ggcacagcaa aatattatcg tactggcgta attaatcagt
     3721 attatcgcta ccgcattcaa ggagcgatta atgcctataa cagcggtaag gtaaattatc
     3781 tattactgag cggcgataac gcattgcaaa gttataatga gccgatgacc atgcgcaaag
     3841 atttaatcgc tgctggtgtc gacccatcag atattgttct cgattacgca ggctttcgta
     3901 cgctggattc catcgtgcgt acacgcaaag tgttcgatac taatgatttc attattatca
     3961 cccaacgttt ccactgtgag cgggcattat ttattgcgct gcatatgggg attcaggctc
     4021 agtgttatgc cgtaccgtca ccgaaagata tgctgtcagt acgtattcgc gaatttgccg
     4081 cccgtttcgg tgcgctggct gacctttata tttttagacg tgaaccgcgt tttttagggc
     4141 cgctggtccc tattccggct atgcaccagg tgccggaaga tgcgcagggg tatcccgccg
     4201 tcacacccga acagttactt gaattacaaa agaaacaagg aaagtagtta tggatgtaca
     4261 gcagtttttt gtcgttgccg tttttttcct tatcccgata ttttgtttcc gcgaagcatg
     4321 gaaaggctgg cgcgcaggcg cgattgataa acgggttaaa aatgcaccgg aaccggtgta
     4381 tgtctggcga gcaaaaaatc ccggactctt tttcgcttat atggtggcat atatcggctt
     4441 cggaatttta tctatcggca tgattgttta tcttattttc tatcgttaat attttctctt
     4501 ttctgttaat tattgagaat aattattact tcactcaata agctgcggat ataacattct
     4561 gaaccgcagc tatttgtgaa tcctttcaca gtttaaattt ccccacacgc ttagccttaa
     4621 tatcagcacg caatatttat ttagcgttcg ccttaattac tcatagcatt aaggaagatc
     4681 acatgccgca acaaaattat ctggatgaac tcactccggc ttttacgcct ttactggcga
     4741 ttaaagaagc ctctcgctgt ttattatgtc atgacgctcc ctgtagtcag gcttgcccgg
     4801 cacagaccga tccggggaaa tttattcgct caatctactt tcgtaatttt aaaggcgctg
     4861 ccgagacaat tcgcgaaaat aatgccctcg gtgccgtttg tgccagagtg tgcccgacgg
     4921 agaaattatg ccaaagcggt tgtacccgtg ccggtgttga tgctcccatt gatatcggcc
     4981 gcttacagcg ttttgttact gattttgaac aacaaaccgg gatggaaatt tatcagcccg
     5041 gtactaaaac gctcggcaaa gtcgcgatta ttggcgcagg tcctgccgga ttgcaggcca
     5101 gtgtgacact gacaaaccag ggttatgacg tcacgatcta tgagaaagaa gcacaacccg
     5161 gtggctgatt gcgtaacggc attccgcaat tccggttacc gcaatcagta ctggatgcag
     5221 agatcgcccg tattgaaaaa atgggcgtga ccattaagtg caacaacgaa gtcggtaaca
     5281 cactcaccct tgagcagctg aaagcagaaa accgcgcggt actggtcacc gtggggttat
     5341 caagcggttc cgggctaccg ctgtttgagc atagtgacgt tgagattgcc gtcgacttct
     5401 tgcaacgtgc acgacaggcg caaggcgata tcagcattcc acaaagcgca ttaattatcg
     5461 gcggcggtga tgtcgcgatg gacgtagcca gcacgctgaa agttctcggc tgtcaggcgg
     5521 taacttgcgt agcgcgtgaa gagttagatg agtttccggc aagcgaaaaa gagtttacca
     5581 gcgcccggga gctgggcgtt tcgatcatcg atggattcac gccagtagcc gtggaaggca
     5641 ataaagtcac gtttaagcat gtacggttat cgggcgaact gacgatggcg gcagataaaa
     5701 ttattctcgc cgtcggtcag catgccagac tagatgcctt tgcggagtta gagccgcagc
     5761 gtaacaccat caaaacacaa aattaccaga cccgcgaccc gcaagtcttt gctgctggcg
     5821 atattgttga gggagacaaa accgtggtct atgccgtgaa aaccgggaaa gaagccgccg
     5881 aggcgattca tcactattta gagggagctt gctcatgtta acgaaagatc tttcgattac
     5941 tttttgcggc gtgaagtttc ccaacccgtt ctgcctctct tcttcgccgg taggcaactg
     6001 ctatgagatg tgtgccaaag cctacgacac aggttggggc ggtgtggtgt ttaaaacgat
     6061 cggctttttt atcgccaacg aagtctcgcc gcgttttgat catctggtga aagaagatac
     6121 cggttttatc ggcttcaaaa atatggagca gattgccgag catccgctgg aggagaatct
     6181 ggccgccctg cgtcggctga aagaagatta cccggacaaa gtattgatcg cttcgatcat
     6241 gggggaaaat gagcagcaat gggaggagct ggcgcgcctg gtgcaagaag ctggcgcgga
     6301 tatgatcgag tgtaacttct cctgtccgca aatgacttct catgcgatgg gtagcgatgt
     6361 cgggcaaagc ccggagctgg tagaaaaata ttgtcgggca gtgaaacggg gttccacgct
     6421 gccaatgctg gcgaagatga cgccgaatat cggtgatatg tgcgaagtgg cgctggcggc
     6481 gaagcgcggc ggcgcagatg gcattgcggc gattaacacc gttaaatcca tcaccaatat
     6541 cgatcttaat cagaaaatcg gtatgccgat cgttaacgga aaatcaagta tttccggata
     6601 ttccggtaaa gcggtaaaac cgatcgccct gcgcttcatt cagcaaatgc gcacccatcc
     6661 agaactgcgc gatttcccaa tcagcggtat cggcggcatt gaaacctggg aggatgcggc
     6721 ggagttttta ttgctcggcg cagcaacgtt acaggtcacc accggcatta tgcagtacgg
     6781 ctatcgcatc gtagaagata tggcgagcgg gttatcgcat tatctcgccg atcagggatt
     6841 tgattcgttg caggagatgg taggtctggc gaataacaat attgtcccgg cggaagattt
     6901 agaccgcagt tatattgtct atccccgtat caatcttgac aaatgtgttg gctgtggacg
     6961 ctgttatatt tcctgttacg acggcggtca ccaggcgatg gaatggagcg agaaaacccg
     7021 cacaccgcat tgtaataccg agaaatgtgt gggttgtctg ctttgtggtc acgtctgccc
     7081 ggtgggttgt attgagctcg gggaagtgaa gtttaagaaa ggcgagaaag aacacccggt
     7141 aacgttgtag aacccgcgtc acatccggca tttggttcct gttgccggat gcggcgtgaa
     7201 cgccttatcc ggcctacaaa accacacaga tcatgatgtt atgtgcgttt atgaccgaat
     7261 gcggaccaca ttcacatcat ttcttacgcg cgtatttcag tgaatccagc gctacggcga
     7321 agataataat cgcccctttg atgatgtact gccagtatgg gtttacgccg atatacgtca
     7381 ggccatagtt gatgacggta aaaataatca ccccggtcac cacgccaatc accgtcccca
     7441 caccgccgct gaacgataca ccgcctacca cgcacgccgc gatagcatcc agctcataca
     7501 taaagccgag gttgttggtg gcagagccga tacgtccggc ttctaacatc ccgccaaagg
     7561 catagaacac gccagacaac gcgtagatca tcagcaggtt caggccgacg ttgacaccag
     7621 atacttttgc cgcttccggg ttaccgccaa tggcaaaaat gttcttaccg aagcgggttt
     7681 tgttccacaa cacccagacg aacgccaccg caatcaacgc gtagaaggtg atgtaagaga
     7741 gacggaaact ccccagcgcg acaaagccct gagcaaaggt agagaagcca ctgtcaaaac
     7801 cagaaattgg cgacgccccg acaaagtcgt aatagagcga gttgatgcca tagacgatga
     7861 tcatcgtgcc gagcgtggta atgaacggcg tcacgttgag ataagcgata atcagaccgt
     7921 tgatcaaacc gatcaccgca ccaatggcac agacaatcag aataaccagc gcaatcggca
     7981 tcgtcgccat ttccgggaac actttgttgg cgttatccat ggactgcaat aatgtcgcag
     8041 ccaccactgc cgccagccct acctgacgac cagcagaaag atcggtcccc tgggtgacaa
     8101 ttaaccctgc cacaccgagc gcgataataa tacgcaccga tgactgggtg agaatattac
     8161 ttaagttcaa cagacttaaa aatgttgggt cctggaaaat aataatcgcc agcaaaacta
     8221 aaagaacgac gtaaataccg ccctctttca ggtaagtaag aaaacttttc ttatttaacg
     8281 cactcatggg aagcccctaa tcttaaaggt gcaaagacgc aagacgcaga atttcgtttt
     8341 gcgttgttgt ttttgtatcg acaattccgg aaacgagacc attgctcatg accagaatac
     8401 ggtctgttat ccctaacaac tcaggcattt cagaggagat aataataatc cccttgcctt
     8461 tcttcgccag ttcggcaatt aactgataaa tttcaaactt cgccccgaca tcaataccgc
     8521 gcgtcggttc atcgagcatt aatatttctg gttgcgttag tagccagcga ccaataatca
     8581 ccttttgctg attaccaccg gagagcgaac caatttgcgt ccgatgaccc ggcgttttta
     8641 cccgcatcga atcaatcacc cactgggtat cgcttttcat ccgcgagtta tccagtaaac
     8701 caactttatt tttgtagttg cgaatattgg aaattaacga gttaaaacca atatccagat
     8761 aggcataaat tcccgttgag cggcgctcct cagttaccag tgcaaatccg tggtttatgg
     8821 cttcgttggc attatggtta ttgatctgtt tgccgtgcaa cgtaatggtg ccagccgatt
     8881 tctcgcgaat accaaataac gtctcaacaa tatcggtacg tttcgccccc accagcccgg
     8941 caataccgag gatctcccct ttatgcagat caaacgagac atcgcgaatc gacggctggc
     9001 gcagtgacgt taggttacgc acctcgagga tgacttcgcc cggcttgttt tctttgtcag
     9061 gaaaacgctg attaagagaa cgcccaacca tcatggcgat gatcttgtcc atcgtcagtc
     9121 ctgccagcgg ttcggtggcg atccactgac cgtcgcgcaa tacggtaact tcatcacata
     9181 actggaagat ttcttccatt ttatgcgaga tataaacaat accgcagccg cgctctttta
     9241 atttacgaat aatagtgaac agatgattga cctctttttc ggttaacgaa gaagtcggtt
     9301 catccataat cacaattttc gcgttatagg aaaacgcttt ggcgatttcg atcatctgca
     9361 tttgcgaaac ggataatgtg ccgacgcgcg cacgcggatc gatatcaata tccagttcat
     9421 caaaaatcgc tttggtttcg cggtacattt tgtcctgatc gacaaacatg cctttggtgg
     9481 gatatcgccc cagccacatg ttatccatca ccgaacgttg taataccagg tttaactcct
     9541 ggtgtaccat cgaaatacca ttttccagtg cctctttggc ggaatggaaa tcgatctctt
     9601 taccctggaa taaaatggtg ccggagtctt tttgataaat accaaacagg cattttaata
     9661 atgtcgattt tcctgcaccg ttttccccca ttaatgcatg gatagaatgt ggccggaatt
     9721 ttaaattaac gttatcaagt gccttaacac caggaaagga cttgttgata ccgctcattt
     9781 ccaacaagta ttccccggaa gacggagtcg ttgagctgac catataattt taccttgttg
     9841 gccatacaat aagggcgcag taaacgactg cgcccaatca gtcttatttc ttgctgaatt
     9901 cagccaggtt gtctttatct acgccaacat aaggtacgcg gaccactttg ttgtcgattt
     9961 tccagttggt gccatcagcc gcacctttgc catcggccag gtttttcgcc agatcaaagg
    10021 tcgctttcgc ctggttgtta gcatcgttca gtactgtgcc cgccagtgca ccggatttca
    10081 ccagcgccag cgcttctggc agcgcatcga cgccaaacac cggaatgctg gacttgttgt
    10141 gtgctttcag tgcttctacc gcgcccattg ccatcgcatc gttgttggcg ataaccactt
    10201 cgattttgtt ggcgttcggg ccagacagcc aggcgtccat cttatctttc gcctgagcgg
    10261 tgtcccacat tgcggtatct aactgtaact gttcagtttt gatgccttta tcgttcaatt
    10321 ctttaatcac gtaagtggta cgtgcttctg catccggatg gcccggttca cctttcagca
    10381 gtacgaactg aatctgaccg tctttgttca gatcccaacc ctgattcgcc gcccagtgtt
    10441 tagcaatcaa atcgccctga ataatgccgg actctttgga gtcagtgcca acgtagtagg
    10501 ctttgtcgta gctatccagc gccttacgag acggttcttt gttgaagaaa accaccggca
    10561 cgttttgccc acgcgctttc tcaatcaccg tacccgcagc tgccgggtca accaggttga
    10621 ttgccagtgc cttcacccct ttcgccagca atacgtcgat ctgatcgttc tgcttggact
    10681 ggtcattctg agaatcattc atcagcagct gaacatctgg cgcggctttc gcatcttgct
    10741 caatagcctt gcgcactaca gacataaagt tatcgtcgta cttatagatt gttacaccaa
    10801 tgcgagtatc agcagcgtgt gcagcggcac cgaataacat gctggccatc acagcagaca
    10861 gggttaacac cttcttattc atggtatctc cggtttttct tatgcagggt agtgcttgag
    10921 ataaatgctc ggcggggcag tagagttaat gaagtgttac tgaacgccga agctcacttt
    10981 tttaaattcg ttcttccatg cccggtaacg ctccagaaaa cggctttaat tgttgttatg
    11041 acgctattac ctcggtgaaa gtgattattc actgttacat acgggttaca acgttaaaac
    11101 ggtgcaatca tagctatcac attgttaaga tactgtgaaa tcactcacag attgaaagcg
    11161 gttacatcgc ctgatttgtt gagttagtga tcgccgccgc attctggcgc gttgcgacag
    11221 aatggcgacg cactaacgtc ggcataaaac agtggctggc acgaggatca atattgcctg
    11281 ctgccccctg caaggccagt tcggtggcta atttagccat tgaagcaatg ggataacgca
    11341 cggtcgttaa ttgcgggtcg gtgtaacggg cgatgggaat atcatcgaaa ccgatgattg
    11401 agagatgtaa cggaatcgca atgccattat cttttaatgc tgtcagtgcg ccagcggcca
    11461 tattgtcgtt ataagcaaat atagcggtaa gttgtagatt gcgccccagc agttcaacca
    11521 tcgccgcctc accgcccggc atgtccggcg taccagtgcc aatccagctt tccggcggaa
    11581 taatatcctg ctctttcaac gcactcatcc agcctgcttt acgcatggcg tcatcttcaa
    11641 tgccgtgact ggaagaaaga taaccaatac gttgatgacc gttattcagc aacatgcgcg
    11701 tcgccattcg ggcaccgctg agattatcca ggcaaacgca acgatgggcg taccccggca
    11761 caacgcggtt gattaacacc ataccgggaa tgttatccat aaattgcgcc agttcatcgt
    11821 cactcaatgc ttttgagtga acaatcaacg cattacaacg ctggcgaatt aacacctcaa
    11881 tggcgtgacg ctctttttcc gcttcatgat agctattgcc gattagcacg tatttctgat
    11941 gctgctgagc gactagatcc accgctttta ccagcgcgcc gaaaaacgca tcagaaacgt
    12001 ccatcaccac cacgccaatg gtgtcgctaa cctgagttgc cagcgcctgg gcattggcgt
    12061 ttggccgata atccagctca ctcacggctt tcattactgc ttcacgcgtg tcggcactga
    12121 ccagcgtgct gttattgagc acccgggaaa ccgttgccac agagacgcca gcctaacgcg
    12181 ctacatcacg aatggtgatc atattcacta cctgttatga gattgcagta aatgactgct
    12241 tgctggcggc tattttgtca gcactgaata caggacttcg tgaatcgagt cacagcaatg
    12301 gaaacggtta cagccgtttt gttaacgaat gtgatccaga tcgttatctt gatgttttag
    12361 atattgccgt tccggcagca cgcaaagtta actgacgcca gagccattcc accggcccct
    12421 ggcggaagta acgcagccag ataacagaga agaggatatt cgccagccat accggaataa
    12481 caaacgccag cagctccagg cggtcaaaat gcataaacaa accgaggtgg taaaaaagca
    12541 tggtacaaat cagcgtttgc aatagatagt tggtcagcgc catccgtccg acgcaggcga
    12601 tcgcaagcac cagcttaaag cggctcaatt gcggccagaa gccataaaac agcgacgcat
    12661 agccaatcgc ctgaaacggc gcactcagtt cccgcggcat ttgaagtaag aaggcacacc
    12721 agcgatacgc ccagtccagc tgccattgca gggcgatggc aggaaggtta atggtcaccc
    12781 caatcgccac cagcacaaaa ccagtacgac gatagtgacg taagctgaac tgccctttca
    12841 gccagccgct gcgcatcagc gccgcaccaa tgagcatcat ccctgccagt tgccagccat
    12901 actgtgcgcc cagtgccagt aaactgttgc caacaccatc ggcacgatta ctgatcgctt
    12961 caacgccgcc gtgaagcttc cagtattttt catacaaaat agccgatgca tccggcgtcc
    13021 aggtgcggct ggtctggctg tcggaaatca gccccaataa cagcaaaacg ccaagcccca
    13081 ccagataaag catgacgcct gtattaaaca ggctttttac cgatggcgca tcgcgcacca
    13141 gccgccagca gattaagccc accagcccgt aagccagcag aatatcgccg tcccagaaca
    13201 ataaaccgtg aataaaaccc agcaagacta acagcgttaa ccgcgactgg atccagcgtc
    13261 tgccacgggg cagcaacatt tgcaggcccg caccaaacag cagcgcaaaa agcgtgagga
    13321 atttcacctg gccgatgaga tcgagaaatg cccaggtcca tgcatcctgc ggcgtaatag
    13381 cgccgtacca ggcgggattg agataagccg cctttggtag cccaaaggcg ctgatgttta
    13441 atagcaggat ccccagaatg gcgacgccgc gaacaaaatc gagcgttacg ttgcgctcca
    13501 tggttcctgc cttttaatca gttgtgatga cgcacagcgc gcagaaactc gtggcgcgta
    13561 ttctgactgg atttgaacaa tccaccaaga gaggtcgttg ttgtggcact ggttgcatcg
    13621 cggatgccac gcgctttcac gcagtaatgc accgcatcaa tggagacagc cacgttattg
    13681 gtgcccagca gcgtttgtag cgcaataaga atttgctgcg tcagacgttc ctgcacctgc
    13741 ggacgctggg caaagaactg cacgatacgg ttaatttttg acagaccgat caccgaatct
    13801 ttcgggatat aagccaccgt cgctttgcca tcgatggtaa caaaatggtg ttcacaggta
    13861 ctggtcagag tgatatcgcg cacggtgacc atttcatcga ccttcatttt gttttcaatg
    13921 agggtgattt tcgggaagtt ggcgtaatcc agaccggaga aaatttcatc gacatacatt
    13981 ttagcgatgc gatgcggcgt ttccatcaaa ctgtcatcag ccaggtcgag attcagcagc
    14041 tgcatgattt cggtcatatg accagcaata aggcttttgc gcgtttcgtt atccatttca
    14101 tgcacgggcg ggcgcagcgg tgtttccagt cctcgcgcaa ctaacgcttc atgaaccagg
    14161 gccgcttctt tactgagtga tggcatttat gatttctcct gcaggtgtga cgcctccgcc
    14221 ctgcgtgggg gcaaagttat taagctgatt tacagcctga ttattgtgcg tgaggcggcg
    14281 cacataatcc agtattcaca gcgataatta ttgtaattgc cgctgacttt catcagcaga
    14341 tgttaaaaca tcgttatgca aatacggaag tgaaagttac tcacagcaca ttgaataaac
    14401 ggtatgatga agaaattgca aacaacacaa caaggagcca cgcatggaaa tgctcgaaga
    14461 gcaccgctgt tttgaaggct ggcagcaacg ctggcgacac gactccagta ccttaaactg
    14521 cccgatgacg ttcagtatct ttctcccacc ccctcgtgat cacaccccgc caccagtgct
    14581 gtactggctt tccggattaa cctgcaatga cgagaacttc accaccaagg cgggtgccca
    14641 gcgcgtggcg gcagaactgg ggattgtact ggtgatgcca gacaccagcc cgcgcggcga
    14701 aaaggttgcc aacgacgatg gctacgattt aggccagggc gcaggctttt atcttaatgc
    14761 cacgcaaccg ccgtgggcga cgcattaccg gatgtatgat tatctgcgcg atgaattacc
    14821 ggcgctggtt cagtcgcaat ttaatgtcag cgaccgctgc gccattagcg gtcactcaat
    14881 gggtggtcac ggtgcgctga ttatggcgct gaaaaatccg ggtaaataca ccagcgtttc
    14941 ggcctttgcg ccaattgtga atccgtgcag cgtcccgtgg ggaatcaaag cgtttagcac
    15001 ctatttaggt gaggacaaaa atgcatggct ggaatgggat agttgcgcgc tgatgtatgc
    15061 cagtaacgcg caggatgcga tcccgacgct tatcgatcag ggcgataatg atcagtttct
    15121 tgccgaccag ttgcaacctg cggtattggc agaagccgcg cgccagaaag cgtggccgat
    15181 gacgctgcgt attcagccgg gatatgatca cagttactac ttcatcgcct cttttataga
    15241 ggatcacctg cgcttccatg cgcagtattt actgaagtga aagtccgccc ggttcgccgg
    15301 gcatcttctc atcagaagcg ataatccact gccataaagt aacgacgtcc gtcttcgtta
    15361 tagctgtagt cgtcacgact gagatccttg tcgccaaggt tcagcacgcc tgcgcgcagt
    15421 ttgacgtctt tagtcacctg ccaggccgcg ccggtattcc agatggtata accgcccggt
    15481 gttttagccg tcgcgctgtc ggcgcgtttc tgcccggtat agtgaccaga aacatagaat
    15541 gaccagtctt ccagcgccag cggtttccag tccagcgtac cgttagcagt atggaacggc
    15601 agatcggata gcggtttgtt ttcgccgttg ctgacatcac gaccatcgtt gtaggtgtag
    15661 ttgatcgaca gtttccattc atcgttgaac ggaattttca gttcggtttc cacgccctga
    15721 atacgagctt tgttaacgtt gtagtagcta aataccggta tgcgccgtcc gttagcgccc
    15781 gtctcaaaac caacaaagtt ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg
    15841 atgctgatac gatctttcac atcgttacgg aaaacggtaa cgctggattc aaccccttcc
    15901 agccagcctt cttcacccat gtagtaaagc cccagctccc aactttcgct ggtttctggt
    15961 ttcagatccg ggctacccac aatcttacat gcgccacggc aggaattgct cgtccagtca
    16021 gggctaagtt gcaacagaga aggtgcttta aatgccgtcg cccagccccc tttcaccgtt
    16081 acggtgtcgg tggcgttata aaccaggtag gcacgcggac tccagtgttc accgtaggtt
    16141 tcgtgatcgt ccatacgcac gccggtcgtc agcgccagcg gctcgaagat ccgccattca
    16201 tcttccacaa acagcgcgta ctggctggca gacgttttgg agctggttcc cccggtcagg
    16261 ttcaccgcat cgctaagttt gtcgtgacgc cattcaccgc caaccgtgag aaactgatta
    16321 atcgccgtca gcggcaacgt gtatttgccg tcgaccgtat tgctttcgga agttatcggg
    16381 ctgctgttgc cagggttttt gttctcgact ttctcaccgt agtatttcag ttcgctggtg
    16441 ccgtaatccc aacgcccatt atggctgacg gagtagttct ggcgttccag gcggtttttg
    16501 tccagcgagt cggaatcacg atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga
    16561 ttttgattcg gtgtccaggc aaattcgaca ttgccgtcgc ggctggagaa tccttcaata
    16621 cgcggcgttt ctccggtatc ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt
    16681 gccaggctgc cgtaagcttt cattcccagc acaccatcaa ttaatggtcc actggtaaag
    16741 aactgaccgt tataggtatc accgcgatcg cgatgttcct gaatggtggt atcgacggta
    16801 acggtacccg accatttctg accgattttt ttggtgatga tattcactac accgccgagc
    16861 gcatcggaac cgtacagcga cgacatcggg ccacggacca cttcaatacg ttcgatggaa
    16921 tcgaccggga tccagttcag atcgaaatca ttgtggcgga agacggcatt gcgggagttc
    16981 acgcgtttac cgtcgacgag aatcagggta tagctgctgt ccagaccacg aatactaacg
    17041 cccttacggt tatccccttc gttcgtcagt tgtacgccag gcacttcttt gaggacatcc
    17101 ttcagattct gtaccggttt tcgctgcaga tcttcctggg taatgacgct gatactggcg
    17161 ggtgcatctt taaggttttg ttcaacggaa gatgcagtga caaccatcgt ttcgccatcg
    17221 tcatcgaccg ctaacacagg ccatacacaa gaaatagcgg acaaacacag cccgacccgt
    17281 acgaaagggt tcaacctaaa cattccatat ctccatgagg taactacgaa aataaaatgg
    17341 gtatcgctca catcttcttt acgtcccctt gcgtacggca gcatcgcggt ggacttattt
    17401 gtatgcaggt gatcatccca gaaagccgga ccagcctctg atttgtcagc cttttttgat
    17461 tgtagcgtaa cgataatgca aacgataaca gttatcaatc caaatgttaa attttatacc
    17521 cgcatgattt gtcagggaaa tggaattgaa aaagccctct cggttgagag ggcttagcaa
    17581 ggaagggagg aaacttattt cttatcgttc tgcgggaact tcatttcgct gtagcgtacg
    17641 aagtgagttc ctttaatcag cttgtagccg aaccaaataa tcaggaacag cgggatacca
    17701 atatacgttg ccgctacgcc gccccagtca atggtatctt tcaggaacgc ttcgtagttc
    17761 tggcccaaag tgataatcag acacagaatg aatgcgaaga tcggccccag tgggaagaaa
    17821 cctgaacggt acggcagatc gttaatgtcg tgtccctgca atacgtaacc gcgacggaag
    17881 cgatagtggc taatggcaat ccccagccag gcgataaaac ccgtcatccc ggaggtgttc
    17941 agcagccaca ggtataccgt ctggttgcca aacatggagg tcaggaagca caaaccggca
    18001 atcaccgtcg tcgcatacag cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa
    18061 atgcgcggcg ctttaccgtc acacgccagg gtgtacagca tacgagtaga cgcatacata
    18121 ccggagttac ctgctgacag caccgccgtc agaataactg cgttcatcac cgccgccgca
    18181 gagagcagac ccgcgtgctg gaacaccaag gtgaacggac tgacgctgat gtctttaaca
    18241 tcgttacgca gcaggctcgg atcggtatac ggaataatca ggctgataat caggatcgcg
    18301 aacacataga acaacaggat tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc
    18361 gccggatctt cggactcgcc tgcagcaata ccgatcagct cggttccctg gaaagagaag
    18421 ccgacaatca tagctacgcc gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg
    18481 attgtccagt tgctccagcc cgcaggctgc gcgcctttga agataccgat aatcatcagc
    18541 acgccaacga tgataaagac aataactgtc gtgactttga tcagtgaaaa ccagtattcc
    18601 gcttcaccaa agccacgaac tgagatgtag ttcagcagga agataacgcc gaggaacaac
    18661 gcactccaga tccagcccgg tgtatccggg aaccaccagc tcatgaccag ctgtgctgca
    18721 accaagtcaa cggcaatagt caccgcccag ttgtaccagt agttccagcc cagcgcgaag
    18781 ccaaagcctt cttcaacata gttctgaccg taagtggcaa acgaaccgga aaccggcata
    18841 tatgcagcca attcaccgag actggtcatc aggaagtaaa ccatcaggcc aatcagcata
    18901 tacgagagca atgccccgcc cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa
    18961 agacctgtac cgatggaacc gccaatggca atcatcgtca ggtgacgcgc ctttaattca
    19021 cggcgtaagc ccggcgcttc tgtagtttta gtttcggaac ccatacaaaa atgctatcca
    19081 tcttaaaaat gaggcgcgat tgtagcagac gattggcaat ccttccggca gaaatacacg
    19141 gttataagac accttcatga tcgcccaggg attataagta aagcagccaa tctcttttct
    19201 ggcgaatgca tgctgagggc gctgcgtcac gaaatcgaca cagcaccagc atgttcttgt
    19261 acagcacctt aacgcggtac attcgcggga tcgcaatagt ccagaaagcg ccgtagcgcg
    19321 ttggaaaggt gtttttgccg atgatgtata cgccacaacg tacgcatcag gcgcgggaga
    19381 gggaccgcaa cttcacttaa tgtgcctgcc tgcaattgat cttcaatcac acgtcgcgac
    19441 aggcaactaa ttcccaaccc atgacgcacc gcatgtttga ttgcctcgga gttacctaat
    19501 tccatcgcca tctcaaactt cggtaaatgt gacagcaaca gataatcgac aatctcccgc
    19561 gtgccggaac cgcgttcacg caggatccac ggcgcagcgg ccagctgttc taaggtgacc
    19621 ggaccacgag ccaacggcga agtcggcgcg gcgaaaacca ccagctcgtc ttccagccac
    19681 ggttcagaaa tgatttcagt gctgtggcac ggtccttcaa taaagccaat atcaacgcgg
    19741 aaatccagca ccgcttgcat cacgtcctgg ctgttcccaa cgctaagttc aatcggcaac
    19801 tgcggataat catggcgata acgggcgata actgcaggca gaatgtagtt accgatggta
    19861 ctactggcat agatacgaat cgcgccgttg tcttcgcgaa acagttgttc gatttcaacc
    19921 gcctgttcaa gcaatgccaa cgcacgcgga tagagcagcc gcccgtgttc attaacaacc
    19981 agtcttttcc ccacgcgatc aaacagttgc acgccaagct gcccttccag gtcggtcaag
    20041 gctgcgctca ctgctgattg cgacaacgcc agcatcaccg acgcctgggt ggttgatcca
    20101 cttttcaata cttctgcaaa aacttccaac tgccggaggg tgatgtgcat agtcgcttac
    20161 cacttataaa gattaattat aaatatataa tcaattttat ttttaaacca gttagtcgta
    20221 accttatgcc cggttaaagg agagggttat gacgaatatc accttacaga aacaacatcg
    20281 tacactgtgg cattttattc cggggttagc cctgagtgca gttatcaccg gggtcgccct
    20341 gtggggtggt tccattcccg cggttgcggg tgccgggttt agtgccctca ccctcgcaat
    20401 cttgttgggg atggttttag gcaacaccat ctatccgcac atctggaaaa gctgtgacgg
    20461 tggtgtgctg tttgccaagc aatatttatt acgtctgggt atcattcttt atggcttccg
    20521 tctgacgttc tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg acgtgttgac
    20581 gctgtccagt accttcctgc tggcttgctt cctggggcag aaagtgtttg gtctggataa
    20641 gcacaccagc tggttgatcg gtgccggtag cagtatctgt ggtgctgccg cggtactggc
    20701 gactgagtcg gtagtgaaag cggaagccag taaagtaacc gtggctgttg caaccgttgt
    20761 tatcttcggg accgtcgcga ttttcctcta cccggcgata tatccgctga tgtctcaatg
    20821 gtttagtccg gaaactttcg gtatctacat tggttctact gtgcacgaag tggcgcaggt
    20881 ggtggcggca ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta tttccaaaat
    20941 gctgcgcgtg atgatgctgg ctccgttcct catcctgctg gcggcgcgtg ttaaacagct
    21001 gtctggggcg aacagcggcg agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt
    21061 catcgtcgtt gccatcttta actcgttcca cctgttaccg cagagcgtgg tgaacatgct
    21121 ggtaacgctg gataccttcc tgctggcaat ggcgatggcg gcgctgggtc tgaccactca
    21181 cgtcagcgcg ctgaaaaaag ctggggcgaa acctctgctg atggcactgg tactgtttgc
    21241 ctggctgatt gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac
    21301 cactacatct tgctcctgtt aacccgctat cattaccgtt ttcctccagc gggtttaaca
    21361 ggagtcctcg catgaaatac attggagcgc acgttagtgc tgctggcggt ctggcaaatg
    21421 ccgcaattcg cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac
    21481 gtcagtggcg tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa gccgcctgtg
    21541 aaaaatatca ctacacatcg gcgcaaattc ttccccacga cagttatctg attaacctcg
    21601 gacatccggt cactgaagct ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc
    21661 gttgcgaaca actggggctt tctttgctca acttccaccc tggcagccat ctgatgcaga
    21721 tttcagaaga ggattgcctt gcgcgtattg ccgaatccat caacattgcg ctggataaaa
    21781 ctcaaggtgt aacagcggtg attgaaaaca ccgccggtca gggcagtaac ttagggttta
    21841 aattcgaaca tctcgcggcg attatcgacg gcgtggaaga taaatcccgc gtcggcgtct
    21901 gcattgatac ctgccatgct ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg
    21961 aaaaaacgtt cgctgatttt gcccgtattg tcggctttaa gtatctgcgc gggatgcacc
    22021 ttaacgatgc gaaaagtacc tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag
    22081 gcaatatcgg tcatgatgcg ttccgctgga tcatgcaaga cgaccgtttc gacggcattc
    22141 cgctgatcct tgaaaccatc aacccggata tctgggcaga agagatcgcc tggctgaaag
    22201 cgcaacaaac tgaaaaagcg gtggcctgat cgatgaacga cagggaaaag cagattctta
    22261 aaattttacg gcgtaacccg ctgattcaac agaatgaaat cgccgatatt ctgcaaatca
    22321 gccgctcacg tgttgccgct catattatgg atttgatgcg caagggtttg attaaaggca
    22381 aaggatatat ccttaccgag caggattact gtgtggttgt gggtgcaatc aatatggata
    22441 ttcgcggaat ggcggatatc cgttacccgc aagcagcctc tcatccaggt tctgttcact
    22501 gttccgcagg cggcgtcgga cgtaatatcg cgcataacct ggctctgctt ggtcgggatg
    22561 tgcatttaat atcagctatt ggcaacgatt tttacggcga gacattactg gaagaaacgc
    22621 gccgcgcggg cgtgaacgtt tcaaactgca ttcgtctgca tggacacagc actgcaactt
    22681 atctcgctat cgctaataag caggaagaaa ctatcctggc aatcaatgac acacatattc
    22741 tgcaacagct aacgcctcaa ctgctcaata cttcacggga cttgattcgc catgctggtg
    22801 tggtgctggc tgattgtaat ctcacacctg aagccctgga gtgggtcttt accattgctg
    22861 atgaaattcc gatgtttgtc gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact
    22921 ggtacagccg gatccacact ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc
    22981 aaccgattaa agacgacaac gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg
    23041 ttaaacgcat ttttgtttat ctgaaagatg aatctgtatt ttgcagcgat aaggatggag
    23101 aacaattttt gctgaccgcg ccagcgcata cgacagtaga cagttttggt gctgacgatg
    23161 gttttatggc gggcctggta tatagctttc ttgaaggata cagtttccgc gacagcgccc
    23221 gttttgcggt agcctgcgcg gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc
    23281 tgtctgccga taacgcgctt tcattagtgc caatggtgta acaatgttgc cggattcgac
    23341 gcttgaccgc atcttatccg gctcataaat tgcgcactac gctaaaccaa taaagaatcc
    23401 tgcaatagta gcactcatca ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag
    23461 ttgggcgatt tccggcgcac ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc
    23521 gatagaacca aagttagcga aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc
    23581 atccagagtg ccacccgttt gcagatatgg cgagaaattg agataagcga caaattcatt
    23641 gatcgccagc ttctgcccaa tcaaactccc ggcaagattt gcatcactcc agtcaacccc
    23701 catcacccac gccaatgggg ccaacaggta acctaaaatc gactccagcg aagcatgttc
    23761 aaaaccaaac cagccgccaa caccgccgat aataccgtta atcaacgcaa ttattgcaac
    23821 aaatgccata acaactgtcg ctacaccggc ggcgattttc agcccggtca ttgcccctgt
    23881 cgcagcggct tcaataatgc tttttggcgg tgtttcggtg aaagagaggt tattaaaaga
    23941 aacctgtgaa gattccgtag ccgggcttaa caggcgggca aacaagatcc cgccagggat
    24001 cgccattaac gatgccgcca gcaaatattc cacaggtacg cccagtgcgg cgtaaccaat
    24061 cattgtcgaa ccagcaatcg aggccatgcc actacaaatc gctgtaaata attcattgcg
    24121 attcagacga tcgataaagg gtttgacgat cgccggaatt tcgttttgcc cgaggaaaat
    24181 ggtggtgacc gcgacgaatg actcgatctt gctgatattt aaagctttct ggaagatacc
    24241 gccgagaatg cgaattaaga tccccatcac accgatgtag tagagaatac tcaccagtgc
    24301 agtgacgaag ataattgccg gtaataccct gaaaccaaag ataaatcctg cgccatcaaa
    24361 taaggtatcc attttcggtc cgaccagaga accgaagata aatgcgctac ctgcgtcgct
    24421 gtacgccatc actttgtgga cgccaaaagc gactttttct gcgacccaac gccctggcgg
    24481 taaccaaagc ataatcccgc caatcacgac ctgtaacact aacgccgcgc caacggtacg
    24541 caggctgatc ttcttcttgt ttactgacag taaaaacgca attgccagca gtaccaccat
    24601 tcccagaaca cttctcatga catccatagt gattatctct tcatgtcatt ttgggcgacg
    24661 gctttctgcc catcagaaat agccgggcct gcatcttgcg atactgtccc ggcttgttat
    24721 caacacttaa tgggttttta tgtagccgcg tacgcactct tcgactaatc cccagaacca
    24781 gtcggtatca atagtgatgc cgactttagt attggcgggc ttgccaagaa cgcccagctc
    24841 gtcgcagacg gtacgcccat agcaagggcc actgttgacg tccacttcga cgtacatctc
    24901 ctgggtttta atgccatcag ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg
    24961 gccgccagcc aggccgtagt tttcgaactg cgttttgaga gtgaagttca tgatgtcgct
    25021 gaacatctct ccggcagggc cgcctgccct ttccattcgt gcaattacgt ccggggtgca
    25081 aacggtctgg ttggtaagat cgaggcccat catcactaat ggaacgccgg aggtgaacac
    25141 cacgcgtgcg gcttccgggt cggcaaagat attgaactcg gcagatgggg tgaagttgcc
    25201 tgtaccgtaa gcgccgccca ttagcacaat ttcacggatt ttgggcagga ttgcgggttg
    25261 catacgcatt gccaccgcga tatttgaaag cggaccaacc ggcaccagag tgatatcgcc
    25321 atcgcttgcc ataagggtat caatgatgta ttttaccgcg tgggtacttt ctgcctggcg
    25381 ggtcagcggc tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac
    25441 gatttgttga cgcataatgg gttgcggcat ccccgcataa accggaacat taatctccag
    25501 tttctggcaa acattcaggc cattaattaa tgttttatca agcgtctgat tacccgcaac
    25561 aatggtgata cctaataaat caatcgccgg atgtttcgcc gccatcatta tagcaatagc
    25621 atcatcatga cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct
    25681 ctgtttccag ttgcgttatt ttttctacag caacgaaatt tcgatcgggc cgatgattga
    25741 atcttaacaa cagcgtacga atgctaaata tgagaaatct catagcggat aaacatcgtg
    25801 aaagaaatcc acaataatga tcttaagcag caattgatga gtgaatccgc gtttaaggat
    25861 tgctttttaa cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac
    25921 attgtgcagg aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg
    25981 ctttacgcca cgctagctaa tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt
    26041 tttattggcg agattgagtt aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt
    26101 gaagagtgtt ggtgccttgc gctccctatg aaacattacc gcccgctgtt attaaacgac
    26161 acgctatttt tacgaaaact ctgcgtcacc ttaagtcata aaaattatcg taatattgtt
    26221 tctttaactc agaatcaatc atttccgtta gtgaatcgcc tggcagcatt tatattactc
    26281 tcgcaggaag gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt
    26341 tcttatcgac atcttttata tgttctcgcg caattcattc acgacggttt attaattaaa
    26401 agcaagaaag ggtatctaat taaaaacaga aagcagttgt caggactggc gctggagatg
    26461 gacccggaga ataaattctc cgggatgatg cagtaaaaat tattggcaat agcacgattg
    26521 ccggatgcaa cgcttaataa ggcgtacatc cggcaatgaa actcaagcta aaccaataaa
    26581 gaacccggca atggtcgcgc tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa
    26641 accaagctgg gcgatttccg gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac
    26701 aacaccgata gaaccaaagt ttgcaaaccc acagagggcg aacgagataa tggcaatggt
    26761 cttgacatcc agcgtgccgc tcgtttgcag atagggcgag aaattgagat aagcaacaaa
    26821 ctcattaatc gccagtttct gcccaatcaa acttccggca agatttgcat cactccagtc
    26881 cacccccata atccatgcca acggggccag cacatagccg aaaatgcttt ccagagagac
    26941 attggcaaaa ccaaaccagc cgccaactcc accaataatg ccgttgatca gcgcaatgat
    27001 cgctacaaat gccattacca ccgtcgccac accggcggcg attttcagcc cggtcattgc
    27061 accgttcgcc gcagcttcaa taatgctttt tggcggtgtt tcgctaaacg acagattttc
    27121 aaacgtaact tgcgaaggtt ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc
    27181 aggaatagcc atcagcgaag cggctaacaa atagtcaatc ggcacgccca ttccggcata
    27241 gccaatcatc atcgaacccg caatagacgc catgccgcta caaatagccg taaatagctc
    27301 gttgcgattc atgcgatcaa taaaaggttt aacgatcgcc gggatctcat tttgcccgag
    27361 gaaaatagtg gtgactgcga caaaagattc gattttgctg atgttgaggg ctttctgaaa
    27421 aatactgcca agaatacgaa tcagcagccc cattacgcca atgtagtaca gcaagctgat
    27481 aagcgcggta acgaaaataa tcgccgggag tacgcgaaag gcgaagataa agcccgcacc
    27541 gtcaaacaga acatccattt tgggaccgac cagcgaacca aaaataaagg cacttcctgc
    27601 gtcactgtag gacatcactt tatggacgcc taatgccgcc tgctctactg cccattttcc
    27661 cgggggaaaa tagagcatga tgccaccaat ggcaatttgc agcagcagcg cagcgccaac
    27721 cgtgcgcaag ctgatgctct ttttatttac cgacaacaca aaggcaattg ccagcaatac
    27781 caccatcccc gcaacacttc tcattatatc cataatgatt ttcccttcat gccagtaaac
    27841 ccggcgtcag cgccaggttt tggtatgttt gatgagtacg ggcgacggct ttctgcccgc
    27901 cagaaattaa cccacgagac gctgatattc tttggcaatt tcgctcgcca gaatggcgtt
    27961 attaaagacc agttgaatat tggatttcag gctatcaccg ccggttagct ccgcaacacg
    28021 cgccagcagg aacggtgtac tctctttccc aataacaccc tgttcttcgg cttcagcaac
    28081 ggcttgatca attgctgcat tgatggtttc ttctggcatc gcaaactgtt ccgggatcgg
    28141 gttcgctacc accaggccgc cgttcaaacc ggtttgccac ttcactgcca tcgcgcgagc
    28201 aatttcgcgg gcactgtcaa gacgaatgct gacttcaaat gggctggttc ggcagaaaaa
    28261 cgcaggcagc gcttttgtct gataaccaat taacggtaca ccgaaggttt ctaaatattc
    28321 tgttgttaat cctaaatcga gaatagattt tgcccccgca caaacaacag tgacattggt
    28381 atttgccagt tcctgtaaat cggcagaaat atcgaaggta tgttccgctc cgcgatgcac
    28441 accaccaata cctccggttg caaaaacttt aatcccagcc agtgctgcaa taatcatggt
    28501 tgatgcaacg gtagtcgcgc catttttccc tgcagcaacg acaaaaggta aatcacgacg
    28561 actaactttc gtcacgttat gtccttcacg gcccagcaat tcgatttctt ctttacttaa
    28621 gcccactttc atcacaccgc caataatggc gatagtggct ggtactgcgc cctgtttacg
    28681 aattgtttct tctacttcaa ttgcggtttg ggcattttgt gggaatggca ttccgtgaga
    28741 aattatggtc gattccagcg caacaaccgg ttttttattt tttaaagcat cctgtacttc
    28801 agcggatatt tgtaataatt caggggacag agttaattca gacattctgt gttctccact
    28861 aacgatctaa cattggcaat tgataattca gggttgttgg tatattcaca ggcaagtgcc
    28921 attgacgaac atccctgcgc aaatcgaaca gaatcgataa acggcatacc gtctacccaa
    28981 caggaggcca gccccgccat catggcgtcg cctgccccag taacattgat gacattcgtt
    29041 ttaatagggg ccgaccaccc actttcaccg ttgatatcac tgtagtaaac gccatcccct
    29101 cccatgctca gcaccaagcg gttcaggccg tgttgatgaa accaggcggc gacctttgcg
    29161 acatcttcac gtccggaaag tgcaattccg ctcaacgttt cggcttcaag acggttaggt
    29221 tttaacgtat ggattttgct cagatgatcg cggattttga cgcatttcca tgccgatacc
    29281 ggatcaataa atacaggcgt ttccccgcta ttttccagaa cccagaccag cgcctcttca
    29341 ctaaggttgc aatccgcgac aataaccttt gcaccgcgaa taaactcctg atgctgggca
    29401 agaaattctg cagaaataca gtcactaata ctcatatcat ttatcgcgac aagcatttct
    29461 cctgtattat cgagcagtga taaatagctg gatgtattct cacccggaac aattaagcat
    29521 ttatcgacat aaacaccgga ttgattagtt tgcgccagta atgattgccc ataaaaatca
    29581 ccacctactg cacttaaaag ccaggcattt ttccccagta acgccagatt atgagcgata
    29641 ttccttccca cgccacctgg ggtaaattta atttttccgg gattcgaatc cgcataattc
    29701 aatggatgat gtgaataccc agccacatca atattcgccg aaccaataat tactatgtag
    29761 tccttttcgc gcatagcacg ccccctggca ataaattgcc aacgaagaaa ttgggtaaaa
    29821 tagccattac agtcaataag actattagag cacgtgttca gatttaaaca catgttcata
    29881 ataagcatat ttaacgtaat gtaaatcgat cagaaaaaac gaaatcggcc agatatgaag
    29941 gcggtcacaa agtttgtcta tttatgcagc aacaaaagag aaagttgtga tgtgtaacac
    30001 acaatatggc aggcaagaaa aagggcagag atgtttctgc cctccagatt gttgacaacg
    30061 cgcgctttgt tcatcctgga tgtggcgtaa acgccttatc cgacctacaa aagcgcaaaa
    30121 aattcaatat attgcagggt tcatgtaggt ctgataagca tagcgcatca ggcaattttg
    30181 cgtttgtcat catcggggca gagatgtctc atccccgata atgaagataa attatgcgtt
    30241 tttttctact atttgtgttt ccgggcgttt caggaaggca taggccaaac ccgccaccag
    30301 cgtaccggca ataattgcta ccaggtaacc caataccggc gtaatagcgc cagggatcag
    30361 cagaacaaac agaccaccgt gtggtgccat cagtttcgca ccaatcgcca ttgagattgc
    30421 gccagtcagc gccccaccaa cgatacagca cggtagcaca cgcatcggat cacgagcagc
    30481 aaacggaatt gcaccttccg aaataaagca cagaccaagt accagagcgg ctttgccacc
    30541 ttcctgctgc gctttgtcga atttgcgacg cgccaccatt gttgccagac ccattgccag
    30601 cggtggcacc atacctgccg ccataatcgc cgccatcggg ccataggttt gagtactcag
    30661 cagacccaca ccgaatgcgt acgctgcttt gtttaccgga ccgcccatgt cagtacacat
    30721 catgccaccg aggatcgccc ccagcagaac cgcattcgca gtccccatgg tctgcagcca
    30781 gtgagtcaac ccatcgagaa tgccagcaac cggtttaccg attaggtaga tcatcgccag
    30841 accgaccacc agactggaaa ttagcggaat gatcaggatc ggtttcagcg cctccatact
    30901 ctgtggcagt ttcaattgcg tactgattaa cttcgcaatg taaccagcca ggaagccagc
    30961 aattataccg ccaataaagc cagaaccggt gctgaccgcc agcataccgc caatcagacc
    31021 cggtgtgagg cccggacgat cggcaatgga gaaggcaata taacctgcca gtaccggcac
    31081 catcagcgca aaggctgaac caccaccaat ctgcatcagc gccgctgcca acgtacccgg
    31141 ctctttaaac gcttcgatac caaaagcaaa agaaagcgcg atacacagac cacctgcaac
    31201 aaccatcggc agcatgtaag agacgcccgt cagcaagtga cggtaagcgc ctgcactctc
    31261 tttcttacct tcagtggtcg ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc
    31321 agcaaccgct ttatccagtt cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg
    31381 atacatcggt ttaccagcaa atttcgccag atccacttcg atatctgccg ccacaatcac
    31441 cagatccgct gctgcgactt cttcaggggt gattgcattg cccgcgccaa cagaaccacg
    31501 ggtttcaact ttcacccacc agccacgttt tttcgcttcg gtttcaatgg cttcagccgc
    31561 cataaaggtg tgtgctacgc cagtcgggca agcagtcacc gcaactacgc gtttcggacc
    31621 gctggcagca accggtgctg tcgcagcgac cggcgcagtg taaggtttcg catggccttt
    31681 ggcttcactc aggaacagct cagggtgcgc aactgcccgg gaaatatcac ccagccagac
    31741 atttttaccg ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa tcgccatttc
    31801 tgcgtcgttc ggattgtcga tgatttccag ttttgctttt cgcgccgccg cgcccagcag
    31861 ggtcttcgcc atataggcgc gtgcctgacc gagattagcg tcaataatca gcagcgtttt
    31921 cattatgcct ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg
    31981 ccaactgcgg acgatcggta atacccacat tgctttgact taccgccagg gctgcaacag
    32041 ctgtcgccag acgcagtgtg tgttcactgg attcacgcat cagcaagcca taaatcaggc
    32101 caccaaccat agaatcccct gcgccaacgg tgcttacgac atcgactgac ggtggtttgg
    32161 cgatccattc gccggaggca ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa
    32221 catgcgcaat accttgttca cgtagcgcat gtgcagcttc aatcacatct ttcatttcag
    32281 gcagtttacg gcctgcccag atttccaact cgcggcggtt aggtttcacc agccacggtg
    32341 ccgctttcaa acctgctact aacgcttcac ggctactatc aaagataatg caaggacact
    32401 gactacgcag gcgagtcatc cagtcggtga acgcttccgg gctgacgcct gacggtaagc
    32461 ttccgctgac acagaccata tcgaactgac cgagccagct cagagaatca gtcacaaagc
    32521 gttcccagtc ggcgagggtg acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt
    32581 ctttttccgt cagcttaacg ttaattcggg tgcgcccctg tacaacctgg aaacggttgg
    32641 caatgcccag ctcgctgaac agttgctgaa aaccatcctg attgtcttta cccaggaagc
    32701 cgccaacggt gacatcaatt cccaggtctt ttaatacttt ggccacgttg atgcctttac
    32761 ccgccgcatg cagaccggtg gttttcacca ggttcacttc gccgcgttca atttccgggc
    32821 agaaaccaac aaggtcataa gccggattaa gggtgatcgt agcaacacgt ctgctcatta
    32881 tgcgccctcc ccaagaccag cagcgatagc gtcgccaatg gctttcagcg cctgttcagc
    32941 atctgcaccc tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa gtgccacaac
    33001 tttcatcaga ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg tcacggtaat
    33061 atcactgtta aattgtttaa tggtattgac cagcatggta cctggacgag catgcaggcc
    33121 gtgttcattg cgcaccacaa actccgcgct taacacgtcg tcggtcggcg catcatcgct
    33181 ggtcagcagc gccagcaacg ttgccgcatc cgctttcagc aagcggtcag ctttattgtc
    33241 gagcaataaa tcagcgagac gcttaagaac cgcgatgggc tgatcgtcat tcatcgccac
    33301 actcaccagc atggctgccg tttcgccgtc cacatcaaaa gcatttgccg cacggcttac
    33361 cgcaatcgcg ctacgcagat tgccttcggc gctatcgctc agccagatac cctgtccgag
    33421 attcagcggt tgttcattga tggctttggt gacgaaagtg gcgtcaactg cccccacctc
    33481 tttcagacgc gcagcgttca gcgcctgaag agtcagcaga tcgctggcga cgatatccag
    33541 cgtcagcatt tcgttgtcga gcttcagctg ctcactctgc ttttcgccca tcagtaatgc
    33601 gcgaagttct tctgctgttg ttgctgactt cagttgttca gcaacggaat catcgctcag
    33661 tacgtgggtc agctggcgta gcaggcccag atgttcatcc gagctggcag caataccgat
    33721 tgccacgtac gctacctgac cgtcacccca ggtgacgcct tccgggaact gaaatacctg
    33781 aacgccggtt ttcagcacct gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc
    33841 attgccgagg aacgttgagg tttgctgttc gcgcgccagc atgccattga cgtagccttc
    33901 tgctacatta ccggcctgca ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc
    33961 tccggccttt tcgcccggat ggatgtcctg tacggataac tggaacatag ttctcctctc
    34021 ttgctgaatt gaaacgattc agcctctatg agaaaaaaag cgccaacctg gcttagggtt
    34081 aaagacaaga tcgcgctgaa acgtttcaag aaagcataat acttctgttt cagcacgcaa
    34141 ggaaagccgt aaagttatga gcaaaagttt gatgtgctgc acaatttttt gtcatttttc
    34201 ctaattgctg atgggaaaat ccgttatcag caatttcatt tcagcagcgt cagcagccaa
    34261 tcagcaacag tgaaatggca ttttgattat tcgtggcgct tttgatttcg ctgtctgttt
    34321 attttctgac aagcagcgta aactccgcgt cttcctcttc cagtgatcga cctgcatgca
    34381 taactccccc gcagtctcca gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat
    34441 cgttgccttt ctcaccggta ttgcgggtgc tctgcaaacc ccgacactca gtattttcct
    34501 taccgatgaa gtacatgccc gtccggcgat ggtgggattc ttctttaccg gcagcgctgt
    34561 cattgggatt ctggtaagtc agtttctcgc tgggcgctct gataagcgcg gcgatcgcaa
    34621 atcgctgatt gtcttttgct gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa
    34681 tcgcaactac tttgttttgc tattcgttgg cgtctttctt agcagctttg gctcgaccgc
    34741 taacccgcaa atgtttgccc ttgcccgtga acatgccgac aaaaccggac gtgaggcggt
    34801 gatgttcagc tcttttttac gcgctcaggt ttcactggca tgggtcattg gcccaccgct
    34861 ggcttatgcg ttagcgatgg gtttcagctt tacggtaatg tatctgagcg cagcggtagc
    34921 gtttattgtt tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc
    34981 gctggcgacc ggcacggttg aagcgccgcg ccgtaaccgt cgcgatacgc tactgctgtt
    35041 tgtcatttgt acattgatgt ggggctcgaa cagcctgtac atcatcaaca tgccgctatt
    35101 tattatcaac gaactgcatc ttcccgagaa actggccgat gtgatgatgg ggaccgccgc
    35161 cgggctggaa atcccgacga tgttgattgc cggatatttc gccaaacgtc tgggtaagcg
    35221 tttcttaatg cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc
    35281 gcattcacct gtcattctgt tgggcttgca gctgctaaat gctattttta ttggcattct
    35341 gggcggcatc gggatgctct attttcagga tctgatgccc ggtcaggcgg gttcagccac
    35401 cacgctctat accaacactt cgcgcgtggg ctggatcatc gcaggatcag tggcgggcat
    35461 cgtcgccgaa atctggaatt atcacgctgt gttctggttt gcgatggtga tgattatcgc
    35521 cactctgttt tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc
    35581 gataagccag gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa
    35641 gcctgcacag actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc
    35701 aagctgaatg cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga
    35761 aattgaaggg gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct
    35821 taaggttaac caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag
    35881 tgcgaatacc acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat
    35941 tttcgactac aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact
    36001 gaattacaac ggcaaactgc tgctggttaa ggatattgat attcagtcgc ccactgcccg
    36061 cggtgccgct acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga
    36121 agagcgtttc aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga
    36181 cttctcctat gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta
    36241 taccttcacc aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc
    36301 ggacatgcag gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt
    36361 tgatctggaa atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa
    36421 caaaccggcg acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg
    36481 cgaaaaaatt cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca
    36541 gccgcatgca gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt
    36601 ctattttgcg cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct
    36661 cacacttcat cgcattaaca atccagacca atttcaattg ctgtcatata actttacact
    36721 gtcattgtta atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac
    36781 gctcccgcat catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt
    36841 tcattttggc tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct
    36901 gaatgcccag ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact
    36961 gatgagccag ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg
    37021 caatcaggtg ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt
    37081 agcggcaatt attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac
    37141 cgaaaaaggc tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat
    37201 tattcacgat ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc
    37261 actgaaacgc cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat
    37321 tcccgacaat ggccatgtgg tgaaaaacgc ggtgctggga atggcagaaa aatgttcgcc
    37381 agaactcgcc gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat
    37441 tgttccggct gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga
    37501 tccctgcgcg attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc
    37561 tgggcgtcct gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga
    37621 agagatgaaa ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct
    37681 ctcaggattc gtccatatca gtgattgcat gcaggatcgc gtatttcgcc atgctgccag
    37741 aacattaatg ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca
    37801 atatgcggat aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca
    37861 aatcgcgatg gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca
    37921 tctggggcgt gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta
    37981 cgtcagcggc gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa
    38041 aattcgcgaa cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct
    38101 gcgtgaagtt ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca
    38161 agcctggcaa caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat
    38221 ttaacgattt ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt
    38281 ttctgccttt tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt
    38341 tgtgaccagg accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct
    38401 tcatctgtta gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg
    38461 ggaagtcgga attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc
    38521 cggcggctgc atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact
    38581 gcgtcaggga aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca
    38641 gatcctcgat ctgttaaccg caccagtcta tgaactgtgg atagatctgc gcgccacctt
    38701 gtgcattctc gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg
    38761 tgaccagctg gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga
    38821 aagtgagcaa gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca
    38881 cagtgagcat gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga
    38941 gttgcccgcc agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt
    39001 aagcctgcca gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc
    39061 ctgcggctgg atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc
    39121 gcgacttgca ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg
    39181 aatcaaccgt cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag
    39241 ccgtattgag ctgatttcca gcagcgaagc tgactggaat gctttgcaga gcgcgctgtt
    39301 gaagcttcgt ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga
    39361 gaaaatatga ttaaaaattt gccgcaaata gtgttgttga atattgtcgg cctcgcgctg
    39421 tttctttcct ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt
    39481 ttttatttct ttaatcagaa actggtcgaa agtaaggcct ttttgtggct ggttgcattg
    39541 accaacaatc gcgctttcga cggttgttct ctgctggcga tgggaatgtt gatgctgagt
    39601 ttctggctga aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg
    39661 ctattaactg cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc
    39721 agcccaacat tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg
    39781 aaagatgcct cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg
    39841 gcattcatgt ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg
    39901 gtttttgcat ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt
    39961 tcgatgaccg tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga
    40021 ttaatcacct tttttgacaa atcactaccg ggaaaaaaca aacatttcca aaacaaataa
    40081 ctcacagtaa ttaacatcat caggcttatt tttatagtga ggataatcct gatgatgcgc
    40141 accgtgcttt catccatcgc gcgcaaaaat cattctctaa gtaaatgaat ggattgcatg
    40201 cgtttcactc aatcgtactt taattgacca atcccgctta ttaactttct gtatcacttt
    40261 ttcttataaa aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa
    40321 ctcgttttgc ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca
    40381 gatttggcct taaggaattg tttcaacatg cccaggtaat tagtctcgtg ccgcttggca
    40441 tttttttata acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa
    40501 tctcaaccga ttttgagata tatcttgcgc gggatccccg cgattgcagt agcggttctg
    40561 ctttctgcat gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg
    40621 ggtagtgaaa catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat
    40681 gtcgacgtaa aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt
    40741 ctgggcggca gcactaaaaa aggtatcgat tgttctggtt ttgtacaacg tacattccgt
    40801 gagcaatttg gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct
    40861 gtttcccgca gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga
    40921 cgccatgtcg gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt
    40981 gttattattt ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg
    41041 gttctcagcc gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta
    41101 actgaaagca ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt
    41161 ccgcgtcttg atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt
    41221 caggaaatta acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt
    41281 acctgcattg tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg
    41341 gcctggcata agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc
    41401 gatacctatt ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat
    41461 acctgccagc aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt
    41521 acgtttgtgc tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac
    41581 attccactca atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc
    41641 ttacccggca cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct
    41701 ttgctgaaaa atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt
    41761 tatagttcac ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta
    41821 tctacctttt cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa
    41881 actaaaattg cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac
    41941 gatgtgtggt acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctctgc
    42001 tattacctga tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc
    42061 gaacaatttt acgtggcgta tcaaccggtg gtggatacac aagctttgcg agtaacgggc
    42121 ctggaagtac tgctacgctg gcggcatcct gtcgcgggag aaattccccc ggatgccttc
    42181 attaactttg ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta
    42241 attgcccgcg atgccgcaga attagaaaaa gtgctgccag taggcgtcaa atttggtatt
    42301 aacattgcgc cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc
    42361 acttccctgc ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg
    42421 aaagagcaag aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct
    42481 attgatgact tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat
    42541 tatctgaaaa ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc
    42601 gtacttgacg cggtgctgac gctggcgaaa cgcctcaata tgctgacggt tgctgaaggg
    42661 gtcgaaacgc cggaacaggc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc
    42721 tactggatta gtcgcccttt accgctggac gattttgtcc gctggctgaa gaaaccgtat
    42781 acgccgcagt ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat
    42841 tgcggctaat gatgagtaaa aggaaatcca ttgcagatga ttgtgcgcat actgctgctg
    42901 tttatcgctc tgttcacctt tggtgtgcag gcgcaggcta tcaaggaaag ctatgccttt
    42961 gccgtgctgg gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc
    43021 gccgcgccaa aaggtgggca gataacgttg tcagccctcg gcaccttcga taatttcaac
    43081 cgctatgcac tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgctattt
    43141 acgacttccg atgacgaacc aggcagttat tacccgctga ttgctgaaag cgcacgctat
    43201 gctgacgatt attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt
    43261 tcgcccatta ctgcccgcga tgtagagttt acttttcaaa aatttatgac cgaaggcgtg
    43321 ccgcaatttc gtctggtcta caaaggcacc accgtcaaag ccattgcacc gttaaccgtg
    43381 cgcattgagt tagctaaacc cagcaaagaa gatatgctga gtctgttttc gctgccgata
    43441 tttccagaaa agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt
    43501 gccagtggtc cgtaccgcgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc
    43561 gtgaaagatt actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc
    43621 attcgctacg attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc
    43681 tttgatttgc gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat
    43741 ttcgataaaa aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc
    43801 tggctggcgt ttaatatcca acgtccggta ttcagcgatc gccgggtccg ggaagcaatc
    43861 actctcgcct ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc
    43921 acgaacagtt attttcagaa taccgaatac gcggccagaa attaccccga cgccgcggag
    43981 ctggtgcttc tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac
    44041 cagccgccgg tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa
    44101 cttctcaacg aaacgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt
    44161 cagccgctca gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg
    44221 ttccagcaca gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg
    44281 caaatcacta accgcatgcg cagtcgcgac tatgacatga tgccgcgcct atggcgggcg
    44341 atgccgtggc ccagttccga tttacagatt tcctggtcat cggaatatat caattccact
    44401 tataatgccc ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc
    44461 gcgcagggaa ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg
    44521 tggaattatt acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat
    44581 aaattctccc aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat
    44641 gacgttaata aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct
    44701 tacctgattc gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac
    44761 tttttcatcg tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag
    44821 tttggtaatg ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg
    44881 caaacgggtg tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagaccca
    44941 gaagtgatcg ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac
    45001 ttcaaaatgc tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc
    45061 tcggtgctga cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc
    45121 acgctgatta tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg
    45181 agtcgctttg acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt
    45241 ttgtttgcca tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct
    45301 ctacgcggcc tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat
    45361 tatctgtggc atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg
    45421 accatgctga ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg
    45481 cgtgcgaaag gggtaagtga gaaaaacatt ctctggaaac atgtgttccg caacgccatg
    45541 ctgctggtga ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg
    45601 ctgattgagg tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc
    45661 tcgcgcgatt atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg
    45721 ctgaatattg tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga
    45781 cgttaatgtc gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc
    45841 gtcgcggcta ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg
    45901 aacttatcgc taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt
    45961 tgttgaaaaa ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc
    46021 aggacccgtg gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc
    46081 gctttggtgc taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc
    46141 ggcaaaactg gctgggaacg gatgccaacg gcggcgatgt gctggcacgc attctctatg
    46201 gcacgcggat ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg
    46261 tgctggcggg ggcgctacaa ggctattacg gcggtaaagt cgatctctgg ggacaacgct
    46321 ttattgaagt atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg
    46381 tacagcctaa cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg
    46441 tcggcgtggt gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac
    46501 aggcgcttgg cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg
    46561 tcgcgaccct caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc
    46621 tcgatttcct cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac
    46681 aagggaaaaa taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga
    46741 tattattgtc tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata
    46801 aggcggtgta gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc
    46861 catcagcaaa ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa
    46921 acgctggcgc tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta
    46981 cgcctgctcc cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa
    47041 tcgctgcttc atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg
    47101 atttttcagg aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat
    47161 gaagtgcttt cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac
    47221 tgccttgatc gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag
    47281 ctctccggcg gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa
    47341 ttattaattg ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta
    47401 cagctgttgc gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac
    47461 ctcagcattg tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc
    47521 gagcaaaata acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta
    47581 ctcaacagtg aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg
    47641 gatgttgaac agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg
    47701 gatcataatg tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt
    47761 ttagtgggcg agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt
    47821 acctctcagg gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag
    47881 ctgttaccta ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac
    47941 ccacgactca acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt
    48001 tctgccgcac aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct
    48061 gaaacacgcc accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt
    48121 gccagggcat taattcttaa gccctcactg atcatacttg atgaaccgac atcatcactc
    48181 gacaaaacgg tacaggcgca aatattgacg ctattgaaat cattgcaaca aaagcatcaa
    48241 ctggcctatt tgtttatcag ccacgatttg cacgttgtcc acgcgttatg tcatcaggtt
    48301 atcgtactgc gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccgca
    48361 ccgcagcagg agtatacgcg tcagctactg gcgttgagct gacgcttaaa aaggattgta
    48421 gtttgaaaag ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa
    48481 ctcgtcctgg cgatttacaa acaggcacgg ctcaccttca cactccagca ccgaacatgg
    48541 aacttcgatg tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc
    48601 atccgggcta agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg
    48661 aatcggttca accttcgaac ctgcgaaacc agcagaccag cgaaagttct gcggtagacg
    48721 atggacgatt gagagttgta atgaagtcac ttattttccc cggaagcaca tttacttcac
    48781 aatttattta catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag
    48841 attgatgcct cgcctgcatg gcctctatgg ctctgattta agtaatatct gcgggcgtgg
    48901 tcacgtttgc ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca
    48961 atgatgtgac tttttacata aattgatttt acataaaata aacatatatc gggggaatga
    49021 tagatttgtg gttgacatgc atcaacaaag gaagcctttt agcttcctcg ttgtgcaata
    49081 gatcaccgtt ttttcggtcg actggcgtac agacagaaga gaatggagct ggttgcgcag
    49141 aatgcaattg accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg
    49201 ccaacaattg cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc
    49261 atatggggaa actcatcaag aatgaccgcc atcgcattgg atgacaccat agacacgcag
    49321 cccacaaacg ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg
    49381 atgaccatcc acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac
    49441 gcgccaatgc ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg
    49501 tttagcgcaa agtaataacc aaagttttcc ggtgcaacgt ggttgatttc aatgtagaca
    49561 aacggtccgg cgctcaggaa cgagaacatt ccggcaaagc tgaaaccgct ggccagcata
    49621 taactcagga cacgcttatg acggaacagc gccgcaaagt taccaatagt ggtacgaatg
    49681 tgaaaaggct ggcgaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    49741 cgccaactat cggtgccatc agcggtgcaa tggtcgtcac cagcatgaca aacgacatca
    49801 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    49861 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    49921 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt accgccaaga accaccggct
    49981 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    50041 tataagtact cagggtcatc tgcgtactgc ccgccggtac gccaaactgc gctgaaatta
    50101 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    50161 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    50221 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    50281 gggctaaatc agcatccagc gtaatgccgc caatacgttc acgatgcagc tcaaccacat
    50341 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    50401 gacgaacctg cgttggggta atcacttcca gcaccgccgg cttagtgaga tctttttcgt
    50461 tatgcagctg cacgccttta gcaaattgtt ctgccgtatc gtcagctacg ggtgatacca
    50521 gcgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    50581 gaccatcatc tgtcattagt accagaccgg tggtatcgat atccagccgc cctgcggcgt
    50641 gcagtttcca cgctaccggt tcatctagaa aatagagcac cgttgggtga tcagggtcgt
    50701 ccgtggagca aacataaccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    50761 gcgccagcgg gttgccatcg taagcgacat catgttcagg aagcagtttg aacgctgcat
    50821 tacggacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    50881 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    50941 ctgtaaaaaa cggaggtcgg gtaatgaccc gaatatctga actgttgtct gcccagtata
    51001 gcggtctaat aacatccctc aagggaaaaa gatccgtggc atactattag cagaataatc
    51061 tacctacgcg agaccatgat ttttacactt cgcccatatc agcaagaagc cgtggatgcc
    51121 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggtgca
    51181 ggtaaaagcc tggtgatagc ggaactggca cggctggctc gtggtcgcgt actggtgctg
    51241 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    51301 gccgatattt ttgccgccgg gctgaagcgt aaagagagcc acggtaaagt ggtatttggc
    51361 agtgtgcagt cggtcgcccg taatattgat gcctttcagg gtgaattttc gctgttgatt
    51421 gtcgatgaat gtcaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    51481 cacctgacaa aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    51541 ttgggcaaag gttggatcta ccagttccat tatcacggca tggtacgcag cgatgagaaa
    51601 gcccttttcc gtgactgcat ttatgagctg cccctgcgtt atatgattaa acacagctat
    51661 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat atgatttcag ccgcttgcag
    51721 gcacagagca acgggctgtt cagcgaagcc gatctcaacc gtgagctgaa aaaacagcaa
    51781 cgtattaccc cgcacatcat cagccagatt atggagttcg ccgaaaagcg taaaggagtg
    51841 atgattttcg ccgcgacggt cgaacacgca aaagagattg tgggattact gcctgccgaa
    51901 gatgcagcac tgattactgg cgacaccccc ggcgctgagc gcgatgtgtt aattgaaaat
    51961 tttaaagccc agcgttttcg ctatctggtc aacgtcgcgg tactgaccac cggatttgac
    52021 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    52081 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    52141 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    52201 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    52261 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    52321 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    52381 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    52441 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    52501 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    52561 tattacgatg aagacggtgc ggatgtgagt gagcgtttcc gtctgcaaac acctgcccag
    52621 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catcccgctg
    52681 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tattgcgacc cccggatttt
    52741 gtcgtcgccc gcatgaaagg ccagtactgg caggtgcgtg aaaaagtgtt cgattacgaa
    52801 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    52861 gcgattgagt atagaatctc acccgctttt gcatacgcaa agcagatcac ttacctgttg
    52921 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    52981 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    53041 ggcaatcatc tacggtggca aagaagcgcc gctggctatc gagctggatc acgacaaagt
    53101 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    53161 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    53221 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    53281 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    53341 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    53401 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc agggtatcgg
    53461 tcgccggctc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    53521 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctccggtct gccgggaagc
    53581 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    53641 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    53701 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    53761 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gtgttcaatc
    53821 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    53881 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    53941 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    54001 cgttaacacg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    54061 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    54121 cgtcacgcag gcgaccagtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    54181 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    54241 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    54301 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    54361 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    54421 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    54481 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    54541 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtgatg
    54601 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    54661 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    54721 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    54781 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    54841 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    54901 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    54961 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    55021 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    55081 tttttgtccg tcattctggc aacggcggga atgacgctat tactgatcga tagcgaagtc
    55141 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    55201 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    55261 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    55321 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    55381 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    55441 ctttcgtacc cgatgacggc gcgacgtttt cttgagaagc atggtctgct tgatgcgcag
    55501 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt tcagtatccg
    55561 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    55621 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    55681 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    55741 ggcctgttct atggtatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    55801 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    55861 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    55921 cgcacccaat ccgacgagca gaccgccacg cagtggatca actggctggg ccactacgca
    55981 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    56041 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    56101 aaccgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt tgtgattatc
    56161 actgccggtc ggggtattcc gctgagcgaa gacgaagaaa cctttgactg gtcccacggt
    56221 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgcg
    56281 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca
    56341 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    56401 gttactgcag cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    56461 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    56521 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    56581 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    56641 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    56701 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacctttacg
    56761 gttggttttt ttatatctgc aattaatttg ataaacagac cgtgacacat cacagcctgt
    56821 ttattttctg ttatcagaac gtccagacca ctcccgcctg agtattccac ggcgattcta
    56881 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    56941 accttatccc agcctgataa acgccacggg ttccagccac atcattatta aatttgccat
    57001 cgtcattaac tttcacatgg ttagaatcgg cctattcctg acgcacggcg gctttcagcc
    57061 agggttccaa cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    57121 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    57181 cctgaccatc tgtggtaaag ccggtaaagg cgagataggg gctaacactc cacaatccgt
    57241 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    57301 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    57361 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtgt
    57421 agctatcaat attgcctttg ccgccgcgat caaaaccaat atcagaatga gagtaatcaa
    57481 agaacaagcc gcgaattgtg ctgctttctt cacgggagaa acggctatcg ataccgagcg
    57541 tcaggcccgt caatgtttgc tcaaagccag ctcccgcatc agtggtcacg ttgttgcggg
    57601 tgttaattgc cgaactccac atcgccgtat cgtaattaac gccttttacg ctaccaagac
    57661 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgctgct gccatgttca
    57721 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    57781 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    57841 ccaacgtgaa tgcagcatca ccgccgcccg tcgttaccag tgtaaggcta tctcctgctg
    57901 ccgggctggc ccccgtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccgatga
    57961 cgttgagctg atcgctctga tgattagcca tatcggtacg catataaaat tgccgtttcc
    58021 cgacagggta ttggtggtca acgtaatgaa attttccgca cttgccgtca cagatgaacg
    58081 agtcactggc tcagccatca taatcacatt tgcgccggac atcgcaaggt tggctaagtt
    58141 gaggcgagtg ttttggttcg tcgtagtgat atcggcaatg atggtccatg cgctattttc
    58201 gagcgaaacg tccgcttggc tggtatccac ggcaccatgc gttctaaaag aagcgccctc
    58261 agaaaccact accttaccca ccaacatagc gggtgcataa tttattttcg gcttcatgac
    58321 ctcaagaatg ccatcattcc ctctgactga cacgttaacc attgtgccag cccagacatt
    58381 agcgcggcca ttgttgataa ccatattttc agagatggct ttagaggaat acgtgatact
    58441 tccattctga tctgatcgac cgagatcata tacagcattt gcctgcactt tcgtaccagt
    58501 atcctttccc agcgattgca tagtggcatg ctcatcaagg atagtgtcaa tggcctgagt
    58561 gtcctccata acaatgagcc cggagccatc atccagttca taattttttg acacaccatc
    58621 ctttatacta aattggcctt tactgtttgt accactcact tccagcgcat tcgttgagac
    58681 aattaatttc ccgcctgctt ttttatcaac gccagttgca gtcccgccat ccataacctc
    58741 cagtaagccg ccactatcca cagtggtatt ataagcgaag tcattttctt caactcgcaa
    58801 acttccgccg ttttccaaca gcatattgtt agcaatacca ttcttgattt cgaactgacc
    58861 gagacggttt gttccgaata cctccatgac ccgcgtggtt gttttaatag caccgcctgc
    58921 tttctgatct accgcaaatg ctaatccccc gtctttaacc tccagtaatc ctcccttttc
    58981 gatagcggca aagacggcaa tcccatcttt ttcaacaatt aagcgcccgc ctttaatcgt
    59041 gggatcattc gccgcg
//
LOCUS       DAZOYS010000003        57430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOYS010000003 DAZOYS010000000
VERSION     DAZOYS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.003"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="ACHUN9_000107"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="ACHUN9_000107"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0693632.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3123)
                     /locus_tag="ACHUN9_000108"
     CDS             complement(1498..3123)
                     /locus_tag="ACHUN9_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0693633.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="ACHUN9_000109"
     tRNA            complement(3582..3668)
                     /locus_tag="ACHUN9_000109"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUN9_000110"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUN9_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0693634.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUN9_000111"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUN9_000111"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0693635.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUN9_000112"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUN9_000112"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0693636.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUN9_000113"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUN9_000113"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0693637.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUN9_000114"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUN9_000114"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0693638.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUN9_000115"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUN9_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0693639.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUN9_000116"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUN9_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0693640.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUN9_000117"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUN9_000117"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0693641.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11961..12068
                     /locus_tag="ACHUN9_000118"
                     /pseudo
     CDS             11961..12068
                     /locus_tag="ACHUN9_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUN9_000119"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUN9_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0693642.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="ACHUN9_000120"
     CDS             complement(13280..14245)
                     /locus_tag="ACHUN9_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0693643.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUN9_000121"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUN9_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0693644.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUN9_000122"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUN9_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0693645.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUN9_000123"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUN9_000123"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0693646.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUN9_000124"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUN9_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0693647.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUN9_000125"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUN9_000125"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0693648.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUN9_000126"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUN9_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0693649.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUN9_000127"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUN9_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0693650.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUN9_000128"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUN9_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0693651.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUN9_000129"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUN9_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0693652.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUN9_000130"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUN9_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0693653.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUN9_000131"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUN9_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0693654.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="ACHUN9_000132"
     CDS             21785..22762
                     /locus_tag="ACHUN9_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0693655.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUN9_000133"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUN9_000133"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0693656.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUN9_000134"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUN9_000134"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0693657.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUN9_000135"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUN9_000135"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0693658.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUN9_000136"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUN9_000136"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0693659.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUN9_000137"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUN9_000137"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0693660.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUN9_000138"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUN9_000138"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0693661.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUN9_000139"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUN9_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0693662.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUN9_000140"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUN9_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0693663.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUN9_000141"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUN9_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0693664.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="ACHUN9_000142"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="ACHUN9_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0693665.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUN9_000143"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUN9_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0693666.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUN9_000144"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUN9_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0693667.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUN9_000145"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUN9_000145"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0693668.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUN9_000146"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUN9_000146"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0693669.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="ACHUN9_000147"
     CDS             complement(34631..35560)
                     /locus_tag="ACHUN9_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0693670.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUN9_000148"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUN9_000148"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0693671.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUN9_000149"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUN9_000149"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0693672.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42714
                     /locus_tag="ACHUN9_000150"
                     /pseudo
     CDS             42310..42714
                     /locus_tag="ACHUN9_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42806..43111
                     /locus_tag="ACHUN9_000151"
     CDS             42806..43111
                     /locus_tag="ACHUN9_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0693673.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUN9_000152"
     CDS             complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUN9_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0693674.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUN9_000153"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUN9_000153"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0693675.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="ACHUN9_000154"
     CDS             complement(44520..45209)
                     /locus_tag="ACHUN9_000154"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0693676.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUN9_000155"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUN9_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0693677.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUN9_000156"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUN9_000156"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0693678.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUN9_000157"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUN9_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0693679.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUN9_000158"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUN9_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0693680.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUN9_000159"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUN9_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0693681.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUN9_000160"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUN9_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0693682.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUN9_000161"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUN9_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0693683.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUN9_000162"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUN9_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0693684.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUN9_000163"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUN9_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0693685.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUN9_000164"
     CDS             54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUN9_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0693686.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUN9_000165"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUN9_000165"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0693687.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="ACHUN9_000166"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="ACHUN9_000166"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0693688.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14258 a        15305 c        14261 g        13606 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgcct cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg cgcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAZOYS010000004        57198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOYS010000004 DAZOYS010000000
VERSION     DAZOYS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.004"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            299..1486
                     /gene="ygeW"
                     /locus_tag="ACHUN9_000167"
     CDS             299..1486
                     /gene="ygeW"
                     /locus_tag="ACHUN9_000167"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0693689.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            1544..2740
                     /gene="dpaL"
                     /locus_tag="ACHUN9_000168"
     CDS             1544..2740
                     /gene="dpaL"
                     /locus_tag="ACHUN9_000168"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0693690.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            2798..4009
                     /locus_tag="ACHUN9_000169"
     CDS             2798..4009
                     /locus_tag="ACHUN9_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0693691.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            4062..5447
                     /gene="hyuA"
                     /locus_tag="ACHUN9_000170"
     CDS             4062..5447
                     /gene="hyuA"
                     /locus_tag="ACHUN9_000170"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR0693692.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            5495..6427
                     /gene="arcC"
                     /locus_tag="ACHUN9_000171"
     CDS             5495..6427
                     /gene="arcC"
                     /locus_tag="ACHUN9_000171"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0693693.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6468..8093)
                     /gene="yqeB"
                     /locus_tag="ACHUN9_000172"
     CDS             complement(6468..8093)
                     /gene="yqeB"
                     /locus_tag="ACHUN9_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0693694.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(8141..8911)
                     /gene="yqeC"
                     /locus_tag="ACHUN9_000173"
     CDS             complement(8141..8911)
                     /gene="yqeC"
                     /locus_tag="ACHUN9_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0693695.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            9014..9592
                     /gene="mocA"
                     /locus_tag="ACHUN9_000174"
     CDS             9014..9592
                     /gene="mocA"
                     /locus_tag="ACHUN9_000174"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR0693696.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            9914..13012
                     /gene="ygfK"
                     /locus_tag="ACHUN9_000175"
     CDS             9914..13012
                     /gene="ygfK"
                     /locus_tag="ACHUN9_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0693697.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            13015..14343
                     /gene="ssnA"
                     /locus_tag="ACHUN9_000176"
     CDS             13015..14343
                     /gene="ssnA"
                     /locus_tag="ACHUN9_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0693698.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            14394..15173
                     /gene="ygfM"
                     /locus_tag="ACHUN9_000177"
     CDS             14394..15173
                     /gene="ygfM"
                     /locus_tag="ACHUN9_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0693699.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            15170..18109
                     /locus_tag="ACHUN9_000178"
     CDS             15170..18109
                     /locus_tag="ACHUN9_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0693700.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            18042..18356
                     /locus_tag="ACHUN9_000179"
     CDS             18042..18356
                     /locus_tag="ACHUN9_000179"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0693701.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            18392..18742
                     /gene="ghxQ"
                     /locus_tag="ACHUN9_000180"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             18392..18742
                     /gene="ghxQ"
                     /locus_tag="ACHUN9_000180"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(18811..19299)
                     /locus_tag="ACHUN9_000181"
     CDS             complement(18811..19299)
                     /locus_tag="ACHUN9_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0693702.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(19299..21218)
                     /gene="ygfT"
                     /locus_tag="ACHUN9_000182"
     CDS             complement(19299..21218)
                     /gene="ygfT"
                     /locus_tag="ACHUN9_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0693703.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21654..22732
                     /locus_tag="ACHUN9_000183"
                     /pseudo
     CDS             21654..22732
                     /locus_tag="ACHUN9_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            22734..22859
                     /gene="yqfG"
                     /locus_tag="ACHUN9_000184"
     CDS             22734..22859
                     /gene="yqfG"
                     /locus_tag="ACHUN9_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0693704.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(22856..22927)
                     /locus_tag="ACHUN9_000185"
     CDS             complement(22856..22927)
                     /locus_tag="ACHUN9_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693705.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            22982..23530
                     /gene="idi"
                     /locus_tag="ACHUN9_000186"
     CDS             22982..23530
                     /gene="idi"
                     /locus_tag="ACHUN9_000186"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0693706.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(23573..25090)
                     /gene="lysS"
                     /locus_tag="ACHUN9_000187"
     CDS             complement(23573..25090)
                     /gene="lysS"
                     /locus_tag="ACHUN9_000187"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0693707.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(25100..26198)
                     /gene="prfB"
                     /locus_tag="ACHUN9_000188"
     CDS             complement(join(25100..26122,26124..26198))
                     /gene="prfB"
                     /locus_tag="ACHUN9_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0693708.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(26289..28022)
                     /gene="recJ"
                     /locus_tag="ACHUN9_000189"
     CDS             complement(26289..28022)
                     /gene="recJ"
                     /locus_tag="ACHUN9_000189"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0693709.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(28028..28738)
                     /gene="dsbC"
                     /locus_tag="ACHUN9_000190"
     CDS             complement(28028..28738)
                     /gene="dsbC"
                     /locus_tag="ACHUN9_000190"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0693710.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(28763..29659)
                     /gene="xerD"
                     /locus_tag="ACHUN9_000191"
     CDS             complement(28763..29659)
                     /gene="xerD"
                     /locus_tag="ACHUN9_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0693711.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            29771..30292
                     /gene="fldB"
                     /locus_tag="ACHUN9_000192"
     CDS             29771..30292
                     /gene="fldB"
                     /locus_tag="ACHUN9_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0693712.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(30332..30739)
                     /locus_tag="ACHUN9_000193"
     CDS             complement(30332..30739)
                     /locus_tag="ACHUN9_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0693713.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(30720..30986)
                     /gene="sdhE"
                     /locus_tag="ACHUN9_000194"
     CDS             complement(30720..30986)
                     /gene="sdhE"
                     /locus_tag="ACHUN9_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0693714.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            31229..32209
                     /gene="ygfZ"
                     /locus_tag="ACHUN9_000195"
     CDS             31229..32209
                     /gene="ygfZ"
                     /locus_tag="ACHUN9_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0693715.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(32405..33064)
                     /locus_tag="ACHUN9_000196"
     CDS             complement(32405..33064)
                     /locus_tag="ACHUN9_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0693716.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(33228..33539)
                     /gene="yqfB"
                     /locus_tag="ACHUN9_000197"
     CDS             complement(33228..33539)
                     /gene="yqfB"
                     /locus_tag="ACHUN9_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0693717.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            33584..35017
                     /gene="bglA"
                     /locus_tag="ACHUN9_000198"
     CDS             33584..35017
                     /gene="bglA"
                     /locus_tag="ACHUN9_000198"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0693718.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(35074..35817)
                     /locus_tag="ACHUN9_000199"
     CDS             complement(35074..35817)
                     /locus_tag="ACHUN9_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0693719.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(36083..38956)
                     /gene="gcvP"
                     /locus_tag="ACHUN9_000200"
     CDS             complement(36083..38956)
                     /gene="gcvP"
                     /locus_tag="ACHUN9_000200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0693720.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(39075..39464)
                     /gene="gcvH"
                     /locus_tag="ACHUN9_000201"
     CDS             complement(39075..39464)
                     /gene="gcvH"
                     /locus_tag="ACHUN9_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0693721.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(39488..40582)
                     /gene="gcvT"
                     /locus_tag="ACHUN9_000202"
     CDS             complement(39488..40582)
                     /gene="gcvT"
                     /locus_tag="ACHUN9_000202"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0693722.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            40872..40955
                     /locus_tag="ACHUN9_000203"
     CDS             40872..40955
                     /locus_tag="ACHUN9_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693723.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(41029..42231)
                     /gene="ubiI"
                     /locus_tag="ACHUN9_000204"
                     /gene_synonym="visC"
     CDS             complement(41029..42231)
                     /gene="ubiI"
                     /locus_tag="ACHUN9_000204"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0693724.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEHRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(42254..43432)
                     /gene="ubiH"
                     /locus_tag="ACHUN9_000205"
                     /gene_synonym="visB"
     CDS             complement(42254..43432)
                     /gene="ubiH"
                     /locus_tag="ACHUN9_000205"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0693725.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(43429..44754)
                     /gene="pepP"
                     /locus_tag="ACHUN9_000206"
     CDS             complement(43429..44754)
                     /gene="pepP"
                     /locus_tag="ACHUN9_000206"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0693726.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(44780..45358)
                     /gene="ygfB"
                     /locus_tag="ACHUN9_000207"
     CDS             complement(44780..45358)
                     /gene="ygfB"
                     /locus_tag="ACHUN9_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0693727.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            45526..45855
                     /gene="zapA"
                     /locus_tag="ACHUN9_000208"
     CDS             45526..45855
                     /gene="zapA"
                     /locus_tag="ACHUN9_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0693728.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            45897..46080
                     /gene="ssrS"
                     /locus_tag="ACHUN9_000209"
     ncRNA           45897..46080
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUN9_000209"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            46101..46703
                     /locus_tag="ACHUN9_000210"
     CDS             46101..46703
                     /locus_tag="ACHUN9_000210"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0693729.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(46804..46863)
                     /locus_tag="ACHUN9_000211"
     CDS             complement(46804..46863)
                     /locus_tag="ACHUN9_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0693730.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(47195..47254)
                     /gene="ibsC"
                     /locus_tag="ACHUN9_000212"
     CDS             complement(47195..47254)
                     /gene="ibsC"
                     /locus_tag="ACHUN9_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0693731.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(47483..48715)
                     /gene="serA"
                     /locus_tag="ACHUN9_000213"
     CDS             complement(47483..48715)
                     /gene="serA"
                     /locus_tag="ACHUN9_000213"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0693732.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(48971..49630)
                     /gene="rpiA"
                     /locus_tag="ACHUN9_000214"
     CDS             complement(48971..49630)
                     /gene="rpiA"
                     /locus_tag="ACHUN9_000214"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0693733.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            50060..50953
                     /gene="argP"
                     /locus_tag="ACHUN9_000215"
     CDS             50060..50953
                     /gene="argP"
                     /locus_tag="ACHUN9_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0693734.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            51156..53300
                     /gene="scpA"
                     /locus_tag="ACHUN9_000216"
     CDS             51156..53300
                     /gene="scpA"
                     /locus_tag="ACHUN9_000216"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0693735.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            53293..54288
                     /gene="meaB"
                     /locus_tag="ACHUN9_000217"
     CDS             53293..54288
                     /gene="meaB"
                     /locus_tag="ACHUN9_000217"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0693736.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            54299..55084
                     /gene="scpB"
                     /locus_tag="ACHUN9_000218"
     CDS             54299..55084
                     /gene="scpB"
                     /locus_tag="ACHUN9_000218"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0693737.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            55108..56586
                     /gene="scpC"
                     /locus_tag="ACHUN9_000219"
     CDS             55108..56586
                     /gene="scpC"
                     /locus_tag="ACHUN9_000219"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0693738.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(56583..>57198)
                     /locus_tag="ACHUN9_000220"
     CDS             complement(56583..>57198)
                     /locus_tag="ACHUN9_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0693739.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT        13987 a        15293 c        14378 g        13540 t
ORIGIN      
        1 agtttatcaa actgaagaga gatagcctgc ccctttatct tatttctgat acttagcagc
       61 aaataaataa cgcgataaaa aaagccaaac gttttcgtat tttacaaaca accagaagct
      121 ggcatcaatt tgtgaacaac cccacacatt atccgtcaaa ttagtctttt gcagccgcgc
      181 ggataattct ggcacactta ttgttagtcc caggtatagc tatgaaaaca ccaatcactt
      241 tggcaagtca cagtgaaata aaccactttg cctgtcattc cactaccggg actgtatgat
      301 gaaaactgtt aatgagctga ttaaggatat caattcgctg acctctcacc ttcacgagaa
      361 agattttttg ttaacgtggg aacagacgcc agatgaactg aaacaagtac tggacgttgc
      421 cgcagcatta aaagcactgc gtgctgaaaa catctcaacc aaagtcttta atagtggatt
      481 aggtatttcc gtattccgcg acaactccac ccgtacccgc ttctcttatg cttccgcgct
      541 taacctgctc ggccttgcac aacaagatct cgacgaaggc aaatcacaaa tcgctcacgg
      601 cgaaaccgtg cgtgaaaccg ccaatatgat ctccttctgc gccgacgcta ttggtattcg
      661 cgacgatatg tatctgggcg caggcaacgc ctatatgcgt gaagttggcg ctgcacttga
      721 tgacggttac aagcagggtg tactgccaca gcgtccggct ttagtgaacc tgcaatgcga
      781 tattgaccac ccgactcagt caatggctga cctcgcgtgg ttacgtgaac actttggttc
      841 actggaaaac ctgaaaggta aaaaaatcgc catgacctgg gcctactctc caagctatgg
      901 caaaccgctc tctgtaccac aaggcatcat cggtctgatg actcgcttcg gtatggatgt
      961 caccttggcc catccggaag gctacgacct gatcccggat gtggttgaag tggcgaaaaa
     1021 caatgctaaa gcctccggtg gtagcttccg tcaggtcacc agcatggaag aagccttcaa
     1081 agacgcagac atcgtttatc cgaagtcatg ggcaccttac aaagtgatgg aagagcgtac
     1141 tgaattgctg cgtgcgaacg accacgaagg cttaaaagca ctggaaaaac agtgtctggc
     1201 acagaacgcg caacacaaag actggcattg tactgaagag atgatggaac tgacccgtga
     1261 tggcgaagcc ctgtacatgc actgcctgcc agctgatatc agcggcgtat cctgcaaaga
     1321 aggtgaagtg actgaaggcg tattcgaaaa ataccgtatc gctacctaca aagaagccag
     1381 ctggaagcct tatatcatcg ccgcgatgat cctgtcccgt aaatacgcca aaccaggtgc
     1441 actgctcgag caactgctga aagaagcgca agaacgcgtg aaataacccc tcgggccgat
     1501 ctgctggtcg gccctctatt tccagaggcc aaaaggatag gatatgtccg ttttctcatt
     1561 gaagattgat atcgccgata acaaattttt caacggcgaa acatcaccgc tcttttcgca
     1621 aagccaggcc aaactggcgc gccagttcca ccagaaaata gctggttatc gcccaacacc
     1681 gctttgtgcg ctggacgatc tcgcaaacct ttttggtgtg aagaaaattc tcgtcaaaga
     1741 cgaatcaaaa cgattcggtc tgaacgcctt caaaatgctt ggcggtgcgt acgccatcgc
     1801 tcaattattg tgtgaaaaat atcatcttga tatcgaaacg ctgtcatttg agcacctgaa
     1861 aaatgccatc ggcgaaaaaa tgactttcgc gaccaccacc gacggcaacc acgggcgcgg
     1921 tgtggcgtgg gcagcacagc aactcggaca gaatgcggtg atttacatgc cgaaaggttc
     1981 tgctcaggaa cgcgttgacg ccattctgaa cctcggtgcc gagtgcatcg tcacggatat
     2041 gaactatgac gataccgttc gcctgaccat gcaacacgcg cagcagcacg gctgggaagt
     2101 ggtacaggac acggcatggg aaggttacac caaaatccca acctggatca tgcaaggcta
     2161 cgcaaccctg gcagatgaag ccgtcgagca aatgcgtgaa atgggcgtaa ccccgacgca
     2221 cgttctgctg caagccggtg tcggagcaat ggccggtggt gtgctgggtt atctggtcga
     2281 cgtctacagc ccgcaaaatc tgcacagcat tattgttgaa cctgacaaag ctgactgtat
     2341 ttatcgctcc ggcgtcaaag gcgacatcgt caacgttggc ggtgatatgg ccaccatcat
     2401 ggcgggcctg gcctgtggcg aacctaaccc gctgggctgg gaaatcctac gtaactgcgc
     2461 cacccaattc atctcctgcc aggacagcgt tgccgcatta ggtatgcgcg tgctgggtaa
     2521 tccgtacggc aacgacccgc gcatcatctc cggtgaatcc ggcgctgtcg gtttgggcgt
     2581 tctcgcagcg gttcattatc acccgcaaca tcaaagcctg atggaaaaac tggcgctgga
     2641 caaagatgcc gtagtgctgg ttatcagcac tgaaggcgac accgacgtga agcactaccg
     2701 cgaagttgtc tgggaaggca aacacgctgt agcaccttaa tcaccctatt ggaactactc
     2761 cctgcaaaac ggggagtaaa aaatctggag aaaaataatg gctaagaata ttccattcaa
     2821 actgattctt gaaaaagcaa aagattacca ggcggacatg actcgcttcc tgcgcgacat
     2881 ggttgctatt cccagtgaaa gctgcgacga aaaacgcgta gtacatcgta ttaaagaaga
     2941 gatggaaaaa gtcggcttcg ataaagttga aatcgacccg atgggcaacg ttctcggtta
     3001 tatcggccac ggcccgcgtc tggtggcaat ggacgctcat atcgataccg tcggcattgg
     3061 caacatcaaa aactgggact tcgatccgta cgaaggcatg gaaactgatg agctaatcgg
     3121 tggtcgcggt acttccgacc aggaaggcgg catggcatct atggtttatg ccggtaaaat
     3181 cattaaagac ctcggtctgg aagatgaata taccctgctg gttaccggta ctgtgcagga
     3241 agaagactgc gacggtctgt gctggcagta cattattgaa caatccggca ttcgcccgga
     3301 atttgtggtc agtaccgaac caaccgactg ccagatatac cgtggtcagc gcggtcgtat
     3361 ggaaattcgt attgatgttc agggtgttag ctgccacggt tctgcgccag aacgcggtga
     3421 caacgccatt ttcaaaatgg gtccgattct tggcgaatta caagaactct cccaacgtct
     3481 gggttatgac gaattcctcg gcaaaggcac cctcaccgtt tctgaaatct tcttcacatc
     3541 cccaagccgt tgcgctgtag cagatagctg cgccgtctct attgaccgcc gtctgacctg
     3601 gggcgaaacc tgggaaggcg cgctggacga aatccgcgcc ctgcctgcag tacagaaagc
     3661 taacgcggtt gtttctatgt acaactacga ccgtccgtcc tggactggcc tggtttaccc
     3721 aaccgaatgc tacttcccga cctggaaagt ggaagaagat cacttcaccg ttaaagcact
     3781 ggtgaatgcc tacgaagggc tgtttggcaa agcgccggtt gttgataagt ggaccttctc
     3841 aactaacggc gtatctatca tgggccgtca cggcattccg gtgatcggct ttggcccggg
     3901 taaagaacct gaagcgcatg cacctaacga aaaaacctgg aaatctcacc tggtgacctg
     3961 tgccgcgatg tacgctgcaa tcccgttaag ctggctggca acagagtaat tactcttcac
     4021 ttattcccct ccggttcgcc ggagggtttt tggagtttgc tatgcgcgta ttgatcaaaa
     4081 acggcactgt cgttaacgca gatggacaag ccaaacagga tttgctgatt gaaagcggga
     4141 ttgttcgcca gttgggcaac aatatttcgc cgcagctccc gtatgaagaa attgatgcca
     4201 ctggctgtta cgttttccct ggcggcgtgg atgtccatac gcatttcaat attgatgtcg
     4261 gcatcgcgcg cagttgtgat gattttttta ccggtacccg cgcagctgcg tgtggcggta
     4321 caacaaccat tattgaccat atgggatttg gcccaaacgg ctgtcggtta cgccatcaac
     4381 tggaggttta tcgtggttat gccgcccata aagcggtcat cgattacagc tttcacggtg
     4441 tgatccagca cattaatcac gcaatcctcg acgaaatccc gatgatggtc gaggaaggac
     4501 tgagcagttt taaactctat ttaacctatc aatacaaact caacgatgac gaggttttgc
     4561 aggcattacg ccgcttgcat gaatccggcg cgctgaccac cgtgcacccg gaaaatgatg
     4621 cggctatcgc cagcaagcgg gcggaattta tcgccgcagg gttaaccgcg ccgcgctatc
     4681 atgccttgag tcgccctctg gaatgcgaag cggaagccat cgcccgcatg attaacctgg
     4741 cacaaattgc cggtaacgcc ccgctctata tcgtgcacct gtctaacggc ttaggtctgg
     4801 attatctgcg tcttgcccgt gcgaatcacc agccagtctg ggttgaaacc tgcccacaat
     4861 atctcctgtt ggacgaacgc agttacgata cagaagatgg catgaagttc attcttagcc
     4921 caccgctgcg taacgtacgc gagcaggaca aactgtggtg tggcatcagc gatggtgcga
     4981 ttgacgtggt ggcgaccgat cactgcacct tctcgatggc tcaacgcctg caaatttcta
     5041 aaggcgattt cagtcgctgc ccaaatggct tacccggtgt ggaaaaccgc atgcagttac
     5101 tgttttccag tggcgtgatg acgggacgta taacaccgga acgctttgtt gaattaacca
     5161 gcgcaatgcc cgccaggctg tttggcctgt ggccgcaaaa aggattatta gcgcccggtt
     5221 ccgatggcga cgtggtgatt atcgacccac gtcagagcca acaaattcag catcgccatc
     5281 tccacgacaa cgccgactac tcgccatggg agggttttac ctgtcagggc gcgattgtca
     5341 gaaccttatc ccgtggtgaa acgattttct gtgacggcac ctttacaggc aaagccgggc
     5401 gaggtcgttt cctgcgacgc aaaccgtttg tccctcccgt gctctaacca ctgtcaggga
     5461 tctatacaca atcaagaatg atgagtgagg aaaaatgagt aagaaaattg ttctcgccct
     5521 gggcgggaat gcgctgggcg atgacctggc cggacaaatg aaagcggtaa aaattacttc
     5581 tcaggcaatt gttgatttaa ttgcccaagg acatgaagtt atcgtcactc atggtaacgg
     5641 gccgcaggtg ggcatgatta accaagcctt tgaagctgcc gcaaaaactg aagcacactc
     5701 gccaatgctg ccgatgtctg tctgtgttgc cttaagccag ggttatattg gttacgatct
     5761 acaaaacgcg ttaagagaag aactgctttc tcggggcatt aataaacctg tagcaacgct
     5821 ggttactcag gtcgaagtcg acgctaacga tccggcattc ctcaacccga ccaagccgat
     5881 cggttcgttc tttaccgagc aagaagcaga gcaactgaca aaacagggtt acactctgaa
     5941 agaagatgct ggtcgcggct atcgccgtgt tgttgcctcg ccaaaaccgg ttgatatcat
     6001 tgaaaaagaa acggttaaag ctctggtaga tgcaggccag gtggtgatcg ccgttggcgg
     6061 tggtggtatc cctgttattc gtgaaggtaa ccatctgcgt ggtgccagcg cggttatcga
     6121 taaagactgg gccagcgccc gtttagcaga aatgattgat gccgatatgc tgatcattct
     6181 gactgcggta gaaaaagtgg ccattaactt cggtaaagag aatgaacagt ggctcgatcg
     6241 cctgtcatta agtgatgctg aacgctttat cgaagaaggt cattttgcga aaggttctat
     6301 gctgcctaaa gtggaagctg ccgcctcgtt tgctcgctct cgcgcaggtc gtgaagcact
     6361 gattaccgta ttgagcaaag cgaaagaagg gattgaaggc aaaaccggaa cggtgatttg
     6421 tcagtaattt taaggccggg tgggtaatac catccggcca ttttcattta agccacttcc
     6481 agcacttctt ttgtcgcttt cacgccctga tgcatcaaca tcattaacgc ctcaagtacg
     6541 ccgccgccaa tcgcccgggc tttatcagaa acgctggtga aatcagcggt ttcaccacga
     6601 ggatcgatat caccaatttt aaaaccacca accactgcca ggccgtcgtt caacaagcca
     6661 cgcaccatcc ccgtcaacgg tgctttaatt tcatgctcac caatccaggc aatcacatcg
     6721 ccctctttca ccagatcgcc taatttcacg ttggatcgca taatgcctgc agcaggagca
     6781 cggatcaccc gtcgggtggt atgccccata atattgccag gaacatcggt attctcctgc
     6841 gcacaaccag agtaaatcac ctgaccgagc cagtgcccgc gatttgtttc aattaccgca
     6901 tgacaatcct tccctgcagt aaagcccggc ccaagcgcga ttgttactgg tgccatatct
     6961 gcccgcgttc ccaaattctg tttcgccaga atagcgtcca ccacgcaaag cggtttcagt
     7021 tcatcaagca atgaacaggc gggatctacc atcacaggga tgaatccgcg ttcggtaagt
     7081 ttcatcgctt ccgcagagct ggttgccagg cgagcagtga cgccttcgac cgtcatttcg
     7141 ccatcgaaca cggcctgggc aaacgccacg gtacaacgaa tcactgtcgg tttttccact
     7201 tccagcatga tcactttaaa acccgcatga tacagacgta gcgccacacc actggcgata
     7261 tctcccgcac cgcgaatcac caccagctgg tgtccggaag gatgagaagg tttcatcatc
     7321 agcccacccg gcgcgttatt tttcacccgt aatatttctg ccagcacgct gatggcgatc
     7381 tcctgcggcg tttccgcacc tatgttgtaa ccaacgggcg catgtaaacg ggcaatatgt
     7441 tcttcagcca cgcctttctc acgcaattga cgcaggaaaa gctgaacctt gcggcgactt
     7501 gccagcaacc ccaaccatgc aatgggctgt tcaatgagtt tgtcgagggc ttcacgatcc
     7561 tgattattcg tggcaatcag gacaaaatta tcagggcgaa tatccagttc ttccactgcc
     7621 gcaccaaacg actcagcatg gagaagcgtg gttgatggtg ggaatagttc aggattgagg
     7681 ctttcgcgat aaatatcggc aacggcaata tcaaatccta atagcgccgc actctgggct
     7741 atcgcccggt tgacatgccc cgcgccaatc aacaccagac gtggacgcat accatggacg
     7801 ctgataaata ctgacattgc acctccacaa tctgacccga cagcatccgc accgttacga
     7861 gccatacgtc catggaataa tcgcggctta cgttcctgca atgcctgaag tgactcttca
     7921 atcaccttcc actcaaccat tccgccacca attgtaccga cgatagaacc atcggcgcgc
     7981 actaacattt gtgcagaatg gcggggagtc gagcctcggc tatcaacaat ttgcgccatc
     8041 gcaaacggac aattttgctc ttcgagtttt gcagcctctg tgaaaatatt cataccaacc
     8101 tcaatggata tcctttcagt aacccggaat acccgggccg ctaattcaca aatctgcgcg
     8161 cgattgcagg atgttcttgt atatcgccca gccagattgc ctctacgtcg tgttgttgca
     8221 gcggttgaag gagctcgctt tgtgcaatcg cattctcaca ttgagaaaaa cggttaatga
     8281 accagactcg ccgacaacct tgcggtacgt ttttaaacgc cccctgaggg tggcgaacca
     8341 gcgcaacgag atcgctcagt tgcaaggttg catcaggtgt taacccggta atatcagcaa
     8401 actgcgacca gcgatggaca ttttctgtgc tcactttcgc gcccaaagta tgtcctccca
     8461 tcacagcaat cacgcagcaa ctgcttttgg gtatgcaagg ttcgtgctca tcaggcgctt
     8521 ttaacggcat tccacgcgag ccatctgctt caatgagaat tacgtcacac tctggtcgtt
     8581 gcaccagtgc atcaatcgct tctggcgtaa atccctgcac ttttccctgg ttcgctttcc
     8641 agctgtgaaa acaaaatgaa atgggggatg taagagacgc atgaggaagc atggcgggat
     8701 cacgacagaa aaccacgggc cagtgagacg ttggcataaa catatgtgtc gtcgtcgtga
     8761 ttaacacacg cctgccgcta gcctggagca gttctgccag ccaaaaaagc aagctggttt
     8821 tccccccagc accaacaaca gaaatcacag tgggacgttt ctgcgcacct aaatcaatga
     8881 ctaatgccga tgggtcaact atacttttca cgagtcttta tgacctctct gggataaatt
     8941 atccccaact taatccatca ggaagtaacg caattatcag gcgttattag cccctataaa
     9001 taatggaacc actatgtcag ccatcgactg tataattacc gctgccggat tatcatcaag
     9061 aatggggcaa tggaaaatga tgttcccctg ggaacaggga acaattcttg atacaagtat
     9121 caaaaatgcg ttgcagtttt gtagccgaat tattttagtc accggctatc gtggtaatga
     9181 actgcacgaa cgttatgcga accagagcaa tatcactatt attcacaacc cagattatgc
     9241 gcagggttta ctaacatccg tcaaggccgc agtacccgca gtgcaaacag aacattgttt
     9301 tctcacccac ggtgacatgc caaccctcac catcgatatt tttagaaaaa tctggtcgtt
     9361 acgaaatgat ggcgcaatac tgccactcca taatggcatc cccggccatc cgattttagt
     9421 gtcaaaacca tgcctgatgc aggcaatcca gcgacccaat gtcaccaata tgcgtcaggc
     9481 actccttatg ggagatcatt attccgtcga aatagaaaat gcagaaataa ttttagatat
     9541 tgatactccg gacgacttta ttacagcgca aaaaaggtat actaaaattt agaaaatgta
     9601 gttaaacgat tgcgttcaaa tatttaatct ctccagcact ctttatcata atgataagga
     9661 atcactgatt ttgagaaaag agaaaaagtg ataaatgggc tgacaataat aattttcagg
     9721 agtgatttta attctcattt ggcacctgtg agttcattca caaaaagcct ttaagtttcg
     9781 agccacctca cattttttta tatttcccgc caaacctggc aagagtggtg cgattgttgc
     9841 tctatccccc taaaccaccg gatttctcaa caccggtcac tcaatgatat ctgtataagc
     9901 taaggagagg gttatggggg atattatgcg tcccattccg tttgaggaac ttttgacgcg
     9961 catatttgat gaataccaac aacaacgctc aatctttggt attcccgagc aacagtttta
    10021 ctcacctgta aaaggtaaaa ctgttagcgt cttcggtgaa acctgtgcca ctcccgtcgg
    10081 ccctgccgct ggcccgcaca cgcagctcgc gcaaaatatt gtcacttcct ggctgactgg
    10141 cggacgcttc atcgaactaa aaaccgtcca aattcttgac cgcctggagc tggaaaagcc
    10201 ctgtattgat gccgaagacg aatgctttaa caccgaatgg tctaccgaat ttaccttgct
    10261 taaagcctgg gatgaatacc tcaaagcctg gtttgccctg caccttctcg aagcgatgtt
    10321 acagccttct gattccggta aatcgttcat ctttaatatg agcgtcggtt acaacctcga
    10381 aggtattaag caaccgccga tgcaacagtt catcgacaat atgatggacg catctgacca
    10441 tccgaaattc gctcaatatc gcgatacgct gaataaatta ctccaggatg acgcattttt
    10501 agcccgccac ggattgcagg aaaaacgcga aaacttgcaa gccttacccg ctcgcatccc
    10561 caccagtatg gtgcagggcg tcaccctctc caccatgcac ggctgtcctc cgcatgaaat
    10621 cgaagccatt tgccgctaca tgctggaaga aaaagggctc aacacctttg tcaaacttaa
    10681 cccgacctta ctggggtacg cgcgtgttcg tgagatcctc gatgtctgcg gtttcggtta
    10741 cataggctta aaagaagagt catttgatca cgacctcaag ctgacgcaag cactggaaat
    10801 gctggagcgc ctgatggtac tggcaaaaga aaaatcactc ggctttggcg taaaactgac
    10861 taacactctc ggcaccatca acaataaagg cgcactgcct ggtgaagaga tgtatatgtc
    10921 aggccgtgcg ctgttcccgc tctccatcaa tgttgcagca gttctctctc gcgcctttga
    10981 cggcaaactg cccatttctt attccggtgg tgccagtcag ctgactatcc gcgatatttt
    11041 tgatacaggt attcgcccta ttactatggc aaccgacctg ctgaaacctg gcggctatct
    11101 gcgcttaagt gcctgcatgc gcgagctgga aggctccgac gcctggggac ttgaccatgt
    11161 tgacgtcgaa cgactgaaca gactggcagc agacgcgtta accatggaat acacccagaa
    11221 acactggaag ccagaagagc gtattgaagt ggcagaagac ctgccgctga ccgattgcta
    11281 cgttgccccc tgtgttactg cctgcgctat caagcaagat attccggaat acatccgtct
    11341 gcttggcgaa caccgctatg ccgacgcgct ggaactcatc taccaacgca acgccctacc
    11401 tgccattacc ggtcatattt gcgatcacca gtgccaatac aactgtaccc gcctggatta
    11461 cgacagtgcg ctgaatatcc gcgaactgaa aaaagtcgcg ctggaaaaag gttgggatga
    11521 atataagcaa cgctggcaca aacccgccgg ttctggttca cgccatccgg ttgccgtgat
    11581 tggtgcaggt ccggcgggtc tggcagcagg ttacttcctt gccagagcgg gccatccggt
    11641 tacgctgttt gaacgcgaag ccaatgctgg cggcgtggtg aaaaatatca ttcctcagtt
    11701 ccgtattcct gcagagttaa ttcagcacga tatcgatttt gttgccgctc acggcgtgaa
    11761 atttgagtat ggctgctcac ccgatttaac cgttgaacag ttaaaaaatc agggcttcca
    11821 ctatgttctg attgccaccg gcactgataa aaatagcggt gtgaaactgg cgggcgacaa
    11881 ccaaaatgtc tggaaatcac tccccttcct gcgtgaatac aacaagggta cagcgctcaa
    11941 gctgggcaaa catgtggtcg ttgtcggggc gggtaacacc gcaatggact gcgctcgtgc
    12001 ggcgttacgc gttccaggcg tagaaaaagc aacgatcgtt taccgtcgtt cactacaaga
    12061 gatgcccgca tggcgcgaag agtatgaaga agcgttgcac gacggcgtag agttccgttt
    12121 cctgaataat ccggaacgtt tcgatgctga tggcacctta accttgcgcg ttatgtcgct
    12181 tggcgaaccg gatgagaaag gtcgtcgccg tccggttgaa accaacgaaa cagtaacact
    12241 gcatgtagac agcctgatca ccgccattgg tgaacagcag gatactgaag ccctgaatgc
    12301 gatgggcgtg ccgctggaca aaaacggctg gccagacgtc gaccataatg gcgaaactcg
    12361 tctgactgac gtctttatga tcggcgacgt acagcgcgga ccatcctcca ttgtcgctgc
    12421 tgtcggaacc gcgcgtcggg cgaccgatgc catccttagt cgggaaaata tccgttccca
    12481 ccagaacgat aaatactgga acaacgtcaa tccagcggaa atctatcaac gtaaaggcga
    12541 tatctctatc acgctggtga acagtgacga tcgtgacgcg tttgtcgccc aggaagccgc
    12601 tcgctgcctc gaatgtaact acgtttgcaa caagtgtgtg gatgtctgcc cgaaccgcgc
    12661 caacgtctcc attgcagtcc ctggcttcca gaatagattc cagacgctgc acctcgacgc
    12721 ttactgtaac gaatgcggca actgcgctca gttctgtccg tggaacggta aaccatacga
    12781 agacaaaatc accgtcttca gcctggcgca agactttgat aacagcagca ccccaggctt
    12841 ccttgtggaa gattgccggg tacgagtacg tctgaataac caaagctggg tgttaaacat
    12901 cgacagcgaa ggtcagttta acaacgtacc accggagctg aacgatatgt gccgcatcat
    12961 cagccatgtc caccagcatc atcattatct gctgggccgc gtggaggtgt aatcatgttg
    13021 attctgaaga atgtcaccgc agtgcagtta cacccggcga aagtgcagga aggcgttgat
    13081 atcgctatcg aaaatgacgt gattgtcgct atcggcgatg ccctgacgca acgctacccc
    13141 gatgccagct acaaagagat gcatggtcgg attgtgatgc cgggaattgt ctgctcgcac
    13201 aaccattttt actcggagct ttcccgcgga attatggcaa acatcgcccc ttgcccggat
    13261 ttcatctcaa cgctgaaaaa tctctggtgg cggctcgatc gcgcccttga tgaagagtcg
    13321 ctctattaca gcggactgat ttgttccctg gaagcgatta agagcggatg tacatcggtt
    13381 atcgatcacc atgcctcgcc ggcgtatatc ggcgggtcgc tctccacatt gcgcgacgca
    13441 tttttaaaag ttggcctgcg cgcgatgacc tgttttgaaa ctactgaccg taacaacggc
    13501 atcaaagagt tgcaggaagg tgtagaagaa aacatccgtt tcgcccgttt gattgatgag
    13561 gcgaagaaag cgacaagcga gccgtatctg gtggaagcac atatcggtgc tcacgcgccg
    13621 tttaccgtgc cggatgccgg tctggagatg ctgcgtgaag ccgtgaaagc cacaggccgt
    13681 ggtttgcata ttcacgctgc ggaagacctt tacgacgttt cctacagtca ccactggtac
    13741 ggcaaagacc tgctggcacg actggcgcaa ttcgacctca tcgacagcaa aacgctggtc
    13801 gctcatgggc tgtacttgtc gaaagatgac atcgccctac tcaatcagcg cgatgcgttc
    13861 ctggtgcata acgcccgttc aaacatgaac aaccatgtcg gctacaacca tcaccttagc
    13921 gacatccgca acctggcatt gggaacggac ggcattggtt cggacatgtt tgaagagatg
    13981 aaatttgcct tctttaaaca tcgcgatgcg ggtggtccgc tgtggcctga cagttttgcc
    14041 aaagccctga ctaacggtaa cgaactgatg agccgcaact ttggcgcgaa atttgggctt
    14101 ctggaagccg gttacaaagc tgatttgacc atttgcgatt acaactcgcc gacgccgctg
    14161 ctggcagaca atatcgccgg gcatatcgct ttcggtatgg gctcaggcag cgttcacagc
    14221 gtgatggtca atggtgtgat ggtctatgaa gaccgtcagt ttaacttcga ttgcgattcc
    14281 atttatgcac aagccagaaa agccgctgcc agtatgtggc gtcggatgga tgcgctggca
    14341 taaatgacag atgccctctt cccgcaggga agagggttaa ataaggaaag aagatgattg
    14401 aacaattttt caggcccgac tctgtcgaac aggcgctgga actgaagcgc cgctaccagg
    14461 atgaagccgt ctggttcgcc gggggcagca aactcaatgc tacaccaacc cgtaccgata
    14521 aaaagattgc catttcctta caggatctgg aactggactg ggttgactgg gataacggtg
    14581 cactgcggat tggtgcaatg tctcgcttgc agccactgcg tgatgcgcgc tttattcctg
    14641 cagcactgcg tgaagccctc ggttttgttt actcacgcca tgttcgtaat cagtcgacca
    14701 ttggtggtga aatcgccgcc cggcaggaag agtcagtgct gcttcccgtc ctgctagcac
    14761 tggatgctga actggttttt ggcaacggcg aaacgctgtc aatcgaggac tacctggcct
    14821 gcccatgcga tcgcctgcta accgaaatta tcattaaaga tccgtatcgc acctgtgcga
    14881 cccgcaaaat tagccgttct caggcaggtt taaccgtcgt gacggcagcc gttgcaatga
    14941 cagaccacga cggtatgcga attgcgctgg atggcgtcgc cagtaaagca ctgcgtctgc
    15001 acgatgtcga aaaacaaaat ctggaaggca atgcacttga acaggctgtc gccaacgcca
    15061 ttttcccgca ggaagatttg cggggcagcg tggcctataa acgctatatc acgggagttc
    15121 tggtagccga cctgtatgcc gactgccaac aggctgggga ggaagccgta tgatcatcca
    15181 ctttacttta aatggcgcgc ctcaggagct aaccgttaat ccaggcgaaa acgtgcaaaa
    15241 gctgttgttt aacatgggaa tgcactctgt acgcaacagt gatgatggct tcgggtttgc
    15301 cggttctgac gcaataatct ttaacggtaa tatcgttaac gcgtccttgc ttattgccgc
    15361 acagttagag aaggcagata ttcgtaccgc agaatctctg ggtaaatgga acgagttaag
    15421 tctggttcaa caggcaatgg ttgatgttgg cgtggtgcag tctggttata acgatccagc
    15481 tgcagctctg attatcaccg atcttctcga tcgcatcgcc gcacctaccc gcgaagagat
    15541 cgacgacgcg ctttctggtt tgttcagccg cgatgcaggc tggcaacaat actatcaggt
    15601 cattgaactg gcggttgcac gtaaaaataa tccgcaggcc accattgata tcgctccgac
    15661 tttccgtgac gaccttgacg tcattggcaa gcattatcct aaaactgatg ccgcgaaaat
    15721 ggtgcaggcg aaaccctgct atgttgaaga ctgcgtaacg gctgacgcct gcgtcattaa
    15781 aatgttacgt agcccacacg ctcacgcact gattactcat ctggatgtca gcaaagctga
    15841 agccttaccg ggcgtcgttc acgttattac tcacctgaat tgccctgata tctactatac
    15901 cccggggggt cagagcgcac cggaaccgtc accgcttgac cgccgtatgt tcggcaagaa
    15961 aatgcgtcac gtcggcgatc gtgttgctgc ggtcgtcgca gaaagtgaag aaattgcgct
    16021 cgaagcactg aagctcatcg acgttgaata tgaagtgctt aagccggtaa tgtcgatcga
    16081 cgaagcaatg gcggaagatg cgcctgtcgt gcacgatgaa ccggtggtgt atgttgctgg
    16141 tgcgccagat actctggaag acgataacag ccatgcagcc cagcgcggcg agcatatgat
    16201 catcaacttc ccgatcggtt ctcgccctcg caaaaatatc gccgccagta ttcatggtca
    16261 tattggcgat atggacaaag gctttgccga tgccgatgtg atcattgagc gaacctataa
    16321 ctcaacgcag gcacagcagt gcccgactga aacacatatc tgctttaccc ggatggacgg
    16381 cgatcgtctg gttatccacg cctccaccca ggtaccatgg cacttacgcc gccaggtcgc
    16441 gcgcctcgtg ggcatgaaac agcataaagt tcatgtcatt aaagagcgag ttggcggcgg
    16501 ttttggttcc aaacaggaca tcctgctgga agaagtgtgc gcctgggcaa cctgcgtgac
    16561 cgggcgtccg gtactattcc gctacacccg tgaagaagag tttattgcta acacctctcg
    16621 tcacgtcgcg aaagtcaccg tcaaactggg agcgaaaaaa gatggtcgcc tgacggcagt
    16681 gaagatggat ttccgcgcca acactggccc ttacggcaac cactcactca ccgtaccgtg
    16741 taacggaccg gcgctgtcgc tgccgttata tccgtgcgat aacgtcgatt tccaggtcac
    16801 cacctactac agcaacattt gcccaaatgg tgcttatcag ggttatggcg caccgaaagg
    16861 taacttcgct atcaccatgg cattagcgga actggctgaa cagttacaga tcgaccaact
    16921 ggaaattatc gaacgtaacc gggtacacga agggcaagag ctgaaaattc tcggtgcaat
    16981 cggtgaaggt aaagcgccga cctccgttcc ttccgccgcc agctgtgcac tggaagagat
    17041 cctgcgtcag ggtcgcgaga tgatccaatg gtcgtcaccg aaaccgcaaa atggtgactg
    17101 gcacatcggt cgcggcgtcg ccattatcat gcagaaatcg ggtatcccgg atatcgatca
    17161 ggctaactgc atgatcaaac tggaatcgga cggcaccttt atcgttcatt ctggcggtgc
    17221 ggatattggt actggtctgg atactgtagt gacgaaactg gcagcagaag tgctgcactg
    17281 cccaccgcag gacgtgcatg ttatctccgg tgataccgat catgcgttgt ttgataaagg
    17341 cgcatatgcc tcgtccggta cttgcttctc gggtaacgcg gcgcgtttgg cagcggaaaa
    17401 tctacgggag aaaattctgt tccacggcgc gcaaatgtta ggtgaggcag tggcagatgt
    17461 tcaactggca acgccgggcg tcgtgcgcgg caagaaaggc gaagttagtt tcggggatat
    17521 tgcccataaa ggcgaaaccg gcatcggctt tggttcactg gtgggaactg gcagttatat
    17581 cacgcctgat ttcgccttcc cgtatggcgc aaacttcgct gaagttgccg tcaacacgcg
    17641 tacgggtgaa atccgcctgg ataaattcta cgccttgctg gactgcggta caccggtcaa
    17701 cccggaatta gcattgggac aaatctacgg tgctaccctg cgagctatcg gccacagtat
    17761 gagcgaagag atcatttatg acgccgaagg tcacccgtta acgcgtgatt tacgcagtta
    17821 cggcgcaccg aaaattggtg acattccgcg tgatttccgc gctgtgctgg tgccgagcga
    17881 cgataaagtc ggcccgttcg gggcgaaatc gatctcggaa atcggtgtaa atggcgcagc
    17941 tccggcgatt gctaccgcaa ttcacgatgc atgcggcatc tgggcagaag aaagtcaaag
    18001 tgggcatggg aacggatatc ggtgccggaa caactttcaa catgctgcaa acgctgaacg
    18061 aagcctacaa agtgttgcaa ttacaaggct atcgcctctc ggcatatgaa gcgttttacc
    18121 tggccacgct cggcggagcg aaatctctgg gccttgacga tttgattggc aactttttac
    18181 ctggcaaaga ggctgatttc gtggtgatgg aacccaccgc cactccgcta cagcagctgc
    18241 gctatgacaa ctctgtttct ttagtcgaca aattgttcgt gatgatgacg ttgggcgatg
    18301 accgttcgat ctaccgcacc tacgttgatg gtcgtctggt gtacgaacgc aactaataat
    18361 aaaactttaa catcctcgtg aggacatcat tatgtctgga gacatcctac aaacaccgga
    18421 cgcaccaaag ccacagggcg cgctggataa ttattttaaa attaccgctc gtggcagtac
    18481 cgttcgtcag gaagtactgg ctggcttaac gacctttctg gccatggttt attccgttat
    18541 cgtcgttccg ggaatgctgg gcaaagcagg ttttcctccc gcagctgtgt ttgttgccac
    18601 ctgtctggtc gcgggcttcg gctcgttgct gatggggttg taggccaatt tgccaatggc
    18661 gattggttgt gcgatttcct tgacggcatt taccgcattc agtctggtac tcgggcaaca
    18721 aattagcgtt cctgtcgcac tggtcgcatt ttacgcgggt ggttgggcaa tctaatagat
    18781 tctccgcgcc tttcagcgcg gagatttctt tcacagagga ttctcaccgc tggcaacaat
    18841 acgctgctgg cggactcgcc tgagttctct ttcagtaagc aactgcagcg cctgtgtcgg
    18901 gcaagattca acacaggctg gcccctcttc ccgctgttca cagagatcgc atttcacaat
    18961 ttgttgtcgg gtatcccccg gcaacgactg aatggtgatc attccaaacg ggcacgcact
    19021 gacgcaactc tgacagccaa tacaacgggc agaattggtc tgcaccacct gctcgcccat
    19081 cgtcagcgcc cccacggggc aagcgccaac acaaggggcg ttttcacact gatggcacat
    19141 caccggcgca ctgatgctgt ccagtcgctg taccttcagc cggggtaaaa agacatcggc
    19201 attcaactcc tgttctgaag ggtgagcgac cacacaggct acttcacaag tacggcagcc
    19261 aatacagtca gccggattaa cgataattaa cgatttcatc acgatgcctt cgtatcaaac
    19321 agagttaaca tatcgcgcgc cgcctgcctt cctgcggcca ttgcagtgac aaccagatcc
    19381 gcgccatgaa ctgcatcacc accagcaaag acttttttca gatgcgtctg ggtaggtaaa
    19441 tacccgacgt caccggtttg aatcaggccc catttatcga gtttaattcc gctgccctgc
    19501 aaccacggca tggcatgcgc ctggaaacca aaggccataa tcagaacatc ggcgggcaat
    19561 tcaaactctg aacccgctac cggacgagga cgacggcgac catccggccc cggctcaccc
    19621 atggcggtac gaatcaggcc caccgcagtt aaacgcccat cttcgtcaca agcgatatat
    19681 tgcggttgaa cattgaactg aaactcgaca ccttcctcgc gcgcattaac cacctctttg
    19741 cgcgagcccg gcatactgac ttcatcacga cgatacgcgc aggtcacgct ggcagcattg
    19801 aggcgaatgg aagtccgcaa acaatccatt gtcgtatcgc caccgcccaa taccacgact
    19861 cgcttacctt ccacgtccgt cagcggatac tcttcagact ccggcaatcc catgagctgg
    19921 cgggtatggg cagtcaggaa cggtagagcc tgaatgacac cgggcgcatc ttcatgcggc
    19981 agatctgctc gcatcatccc gtaagtcccc acgccgatga aaactgcatc atattcagaa
    20041 gttaaatcgc taaaggtaat atcgcggcca atttcacagt taagatgaaa gtcgattccc
    20101 attgcggtga atatctctcg ccgctggctt aataccgttt tatcgagttt gaaaggagga
    20161 atgccaaaag tcagcatacc gccaatttct ggatggcgat caaagacatc aacctgaact
    20221 cccgcgcgcg ccagaatatc agcacaccct aaccctgctg gcccagcgcc aatcaccgcc
    20281 actttttcga tacggggaac aactttgctg acatcgggac gccagcccat cgccagcgcg
    20341 gtatcggtga tgtagcgttc cagattaccg atagagactg cgccagagtg atctttcaaa
    20401 gtacatgcgc cttcacaaag acggtcctgt gggcataccc tgccgcagat ttctggtaag
    20461 gaactggtct ggtggcaaag ttctgccgct tcaataatct ttccttcctg taccagacgg
    20521 atgtaatccg gaatagcgtt atgcagcggg caatgccagt tgcagttagc tttttcggca
    20581 caataaacac agcggtcact ctcataagtc gcttgcagtg gatccagccc gcaatagatt
    20641 tcgccaaagt gggttttccg ttcactcgct gaaattttat ctgcgccttt acgcgagtta
    20701 acggggagca acgctgcact gcgagatggc tgagcgtctg atgacgcttt tcctgctgcc
    20761 gttttgcgct ggcgagccac ctttatccac tgtaaccctt tatcgtccat cagtcgtaac
    20821 gcctgtgttg ggcagacttc aatacaggct tgcgtgccgg aactgcgctg gttacaaagg
    20881 tcgcatttct gcgcaatcgt atcgaccatc tcaacgacgc caaaggggca agcgattgcg
    20941 catcttttac agccaataca tttttgctcg tccagttgta cgctatcgga ctggaaagtc
    21001 agagcattaa ccggacaagc cgtaacacaa ggggcattgt tgcagtgatg gcaggccacc
    21061 ggattcgcag cctggccttt ccctacaacg tggatacgcg gtcgaaagtc actgtgactc
    21121 agcggccagt tttcttgatt gtgtgccacc gcacaggcaa tttcacaagc atgacagcct
    21181 atacattccg cagcttcagc agcgataaac ttattcattt gcatcccttt catttaatga
    21241 gttatgtctt ctcaaatttc gcgatgcaat aaacgggcat atattaggta gcatgaccgt
    21301 tttaattata attttgcgac tgagttcaaa ttattgcccc tcaatctatg ccgctttcat
    21361 tatttcaaca ttgccgatca aaagagcgct atccagataa aaacgattaa atgctagagt
    21421 gcgacatgcc agaatgatta atgaaatatc aatatgagaa aattacagat aattccattg
    21481 tcaggttctc ttgtcatttt cgtggtttaa gcacgattat gtgattgcct ccgcaattcc
    21541 tgtctctaac tccccttcct cgcaaaaact ggcactccac gagcatgtgt ttagacagtt
    21601 tcattaacgt aaacggttgc tttttactct ggcgggcgaa aggagaaaca ctgatgagcg
    21661 ccatagattc ccaacttccc tcatcttctg ggcaagaccg cccaactgat gaggttgacc
    21721 gcatattatc accaggaaag ctgatcatac tcggtctgca acacgtcctt gtcatgtacg
    21781 caggtgcagt cgctgttcct cttatgattg gtgaccgact gggcctctca aaagatgcta
    21841 ttgcgatgct cattagctcg gatctctttt gctgcgggat cgtcacatta ttgcaatgta
    21901 tcggtatcgg ccgctttatg gggatccgcc tgccggtgat tatgtcggtg acctttgctg
    21961 ctgtaacacc aatgatagcc attgggatga acccggatat cggcctgctg gggatatttg
    22021 gtgccactat cgccgcgggt tttatcacca cattattagc gccatttatt ggtcgcttga
    22081 tgcctttatt cccgccactg gttaccggtg tggttattac ttctattggg cttagcatca
    22141 ttcaggtggg tattgactgg gccgccggag gtaaagggaa tccgcaatat ggtaatcccg
    22201 tttatttagg tatctccttt gctgtcttaa tttttatctt gctcattact cgctatgcga
    22261 aaggatttat agccgctggg tgtgtatttc ttcgggaatt atattaatcc tgtttggcat
    22321 ggtgccaaaa atggcggtgc tggtcgcctc cattccgcaa tttgtgctgg gcggtgctgg
    22381 gctggtgatg ttcggcatgg tactggcgac agggattcga attctgtcgc gctgtaacta
    22441 caccaccaac cgttacaacc tctatattgt ggcgatcagt ctcggcgttg gcatgactcc
    22501 gacgctctct cacgatttct tttctaagtt accggccgta ctgcaaccgt tgctgcatag
    22561 cggcattatg ctcgcaaccc ttagcgccgt tgtgctgaat gtcttcttta atggctacca
    22621 gcatcatgct gacctggtga aggaatccgt ctctgataaa gatttaaaag tcaggacagt
    22681 acgtatgtgg cttctgatgc gcaagctgaa gaaaaatgag catggagaat aatatgaatt
    22741 ttttaatgcg cgttatattc agtctgctgt tgctttttac tctctctatt cctgtcattt
    22801 ctgactgtgt tgcaatggcc attgaaagtc gcttcaaata tatgatgcta cttttttaaa
    22861 tggtttttac ctgtcggcat ccgctcaaaa cgggcggttg tcgataaacg ctcacttggt
    22921 taatcatttc actcttcaat tatctataat gatgagtgat cagaattaca tgtgagaaat
    22981 tatgcaaacg gaacacgtca ttttattgaa tgcacaggga gttcccacgg gtacgctgga
    23041 aaagtatgcc gcacacacgg cagacacccg cttacatctc gcgttctcca gttggctgtt
    23101 taatgccaaa ggacaattat tagttacccg ccgcgcactg agcaaaaaag cgtggcctgg
    23161 cgtgtggact aactcggttt gtgggcaccc acaactggga gaaagcaacg aagacgcggt
    23221 gatccgccgt tgccgttatg agcttggcgt ggaaattacg cctcctgaat ctatctatcc
    23281 tgactttcgc tatcgcgcca ccgatccgaa tggcattgtg gaaaatgaag tgtgtccggt
    23341 atttgccgca cgcaccacca gtgcgttaca gatcaacgat gatgaagtga tggattatca
    23401 atggtgtgat ttagcagatg tattacacgg tattgatgcc acaccgtggg cgttcagtcc
    23461 gtggatggtg atgcaggcag ccaatagtga agcaagaaaa ttgttgtctg ctttcgcgca
    23521 gcacaattaa aaaaccccga catttgccgg ggttgtgagc ataacgtaat gcttatttta
    23581 ccggacgcat cgccgggaac agaataacgt cgcggatggt atggctgttg gtgaacagca
    23641 ttaccatacg gtcgatgcca atccccagac ctgctgtcgg cggtaagcca tgttccagtg
    23701 cggtaacgta atcttcatcg tagaacatcg cttcgtcgtc acctgcgtct ttcgcggcaa
    23761 cctgatccag gaagcgttgc gcctgatctt ccgcgtcatt cagctcgcta aagccgttac
    23821 cgatttcacg accaccgatg aagaactcaa agcggtctgt gatttccggg ttaatgtcgt
    23881 tacgacgcgc cagcggagaa acttctgccg gatattcagt aatgaaggtc ggctgaatca
    23941 gatgtgcttc tgccacttct tcgaagatct cggtaacgat acggcccaga ccccagctct
    24001 tctcaacgtg gataccgata gattcagcaa tcgctttcgc agagtcgaag ttatccagat
    24061 ccgccatgtc ggtttccggg cgatatttct tgatcgcttc acgcatggtc agtttttcga
    24121 acggtttacc gaagtccagc gtcacgtcgc cgtaggtcac ttccgtctta ccgagaatat
    24181 cctgtgccag agtacggaac agcgattcgg tcagctcgat cagatctttg taatctgcgt
    24241 aagccatgta gagttccatc atggtgaact ctgggttatg acgtacggaa ataccttcgt
    24301 tacggaagtt acggttgatt tcgaatacac gctcgaagcc accgaccacc agacgcttga
    24361 ggtacagttc cggcgcgata cgcaggtaca tgtcgagatc cagcgcgtta tggtgggtga
    24421 taaacggacg cgcagcggca ccgcctggga tcacctgcat catcggcgtt tcaacttcca
    24481 taaagccgcg gttcaccatg aactggcgaa taccagagag gatctgcgag cgtactttaa
    24541 aggtgttacg ggattcatcg ttagagatga gatccagata acgctgacga tagcgcgctt
    24601 cctgatcctg caagccgtgg aatttatccg gcagcggacg cagtgctttg gtcagcagac
    24661 gcagctcggt acagtggata gacagttcgc cggttttggt tttgaacagt ttacctttcg
    24721 cgccgaggat gtcgccgagg tcccattttt tgaactgctc gttgtaaacg ccttccggca
    24781 gatcgtcacg ggcaacgtac agctgaatgc ggccacccac atcctgcagg gtaacgaaag
    24841 acgctttacc cataatacgg cgggtcatca tgcggccagc aacggcgact tcgatgttca
    24901 gcgcttccag ttcttcgttc tctttaccgt cgaattctgc gtgcaattgg tcagaggtat
    24961 gatcgcgacg gaaatcgttc gggaaggcaa tcccctgctc gcgcaggttc gccagcttct
    25021 cacgacgcgt tttcagttca ttgttaagat cgactaccgc gtcagcgccc tgtgcgtgtt
    25081 gttcagacat gttggttcct cataaccctg ctttcaaact tgcttcgata aattgatcca
    25141 ggctgccgtc cagcacggcc tgcgtgttgc gagtttctac cccggtacgc agatctttaa
    25201 tgcgggagtc atcaaggaca taagaacgaa tctggctgcc ccagccgatg tcagatttgt
    25261 tatcttccat cgcctgtttc tcggcatttt tcttctgcat ctccagttca taaagcttcg
    25321 ctttcatctg cttcatggcc tgatctttgt tcttgtgctg ggaacggtcg ttctggcact
    25381 gggtcacgat cccggtcggg atgtgggtaa tacgcaccgc agattcggta cggttaacgt
    25441 gctgaccgcc cgcgccggac gcgcgataaa cgtcaatgcg cagatccgcc gggttgattt
    25501 cgatatcaat atcatcatca acttccggat aaacaaacgc agagctgaac gacgtgtggc
    25561 gacgaccgcc ggagtcaaac gggctcttac gcaccaggcg gtgaacgcca gtttctgtac
    25621 gcagccagcc gtaagcgtaa tcgccggaga ttttgatcgt cacggattta atacccgcca
    25681 cttcaccttc cgactcttcg atgatttcag ttttgaaacc acgcgattct gcccagcgca
    25741 gatacatacg ctcaagcatg ctcgcccagt cctgtgcttc cgtaccgcca gaccccgcct
    25801 gaatatcgag gtagcagtcg gcgctgtcat attcgccaga gaacatacgg cggaactcaa
    25861 gctgcgccag tttttcttcc agggcgtcga gttcagcaac ggcttcgtta aaggtttctt
    25921 cgtcgtcagc ttctacagcc agttccagca gaccagaaac atcttccagc ccctgtttca
    25981 tttggtcgag ggtgtcgaca acggcttcga gggaggaacg ctctttaccc agcgcctgtg
    26041 cgcgttcggg ttcattccag acatccggct gttccagctc ggcgtttact tcttccagac
    26101 gctctttctt ggcgtcgtag tcaaagatac cccctaagaa cgtcggagcg ttccgtgagg
    26161 tcctgaatgc gattatttac cggattaatt tcaaacatgg tctgatttct tttattgagc
    26221 tagtcaaaat gcggtgataa gagcgggatt gtacccaatc cacgctcttt tttatagaga
    26281 agatgacgct aaattggcca gatattgtcg atgataattt gcaggctgcg gttgccgcga
    26341 aactcgttga tatcgagctt gtaagccagt tgcacttcgc gcacgccgtt atccggccag
    26401 agggcggtat cgacattaaa agcaatacca tccagcagcg gaccgccgcc gaccggttcg
    26461 accatgactt tcaaatgacg ttcgcccacc agccgctgtt gcagcagccg gaaatgaccg
    26521 tcaaacagcg gctccgggaa catctgcccc cacgggccag catcgcgcag cagctgcgcc
    26581 acttccatgg tcatttcggc cgggcttaac ggaccgtctg acaccacttc gccttgcaat
    26641 agcgaagggt ccagccactc agtaaccagt tcgccaaacc gttgttgaaa gagttcgaat
    26701 ttatccgctt ccagcgacaa acccgccgcc atcgcatgac cgccaaactt cagcatcatg
    26761 ccagggtaga gtgtgtctaa tcgctccagc gcatcacgca tatgcagccc ctgaatggag
    26821 cgaccagaac ctttcagcgt accatcaccc gcgggcgcaa aggcgataac cggacggtga
    26881 aaacgctctt taatacggga agccagaata ccgacaaccc cctgatgcca ttcggggtga
    26941 tacattgcca gcccgccggg tagcgtgtca cggctgcgct ccagtttctc gcacagggtc
    27001 agggcttcaa cctgcattcc ttgttcgatc tcttttcgcg tctggtttag cgcatcgagt
    27061 tcatttgcca gcacgcgcgc ttcgccgatg ttgtcgcaca gcaagagcgc cacaccgacg
    27121 gacatatcgt ccagtcgtcc ggcagcattg agacgtggcc ccagcgcaaa acctaaatcg
    27181 ctggcggcga gtttttgtgc atcacggttt gccacttcaa gcagcgcttt aatccccgga
    27241 cggcactttc cggcacggat gcgactcatc ccctgccagg tcagaatgcg attattagcg
    27301 tccagcggta cgacgtccgc cactgtcccc agcgctacca gatccagcag ttctgccagg
    27361 ttaggaattg cgatgccgcg ctcatcaaac cagccctgat cgcgcaaaaa ggtgcgcagc
    27421 gccagcatca gataaaacgc cacacccacg cctgccagtg atttcgacgg gaaattacag
    27481 tcgcgcaagt tagggttaat gatcgcttcc gccgcgggta atgtttcgcc cggcaaatgg
    27541 tgatcggtaa caataaccgg aatgcccaac gagcgagcgt gttcaacccc cgcatgggag
    27601 gaaataccgt tatccaccgt gacaattaac tgcgcaccac gggcatgggc ctgatcgacc
    27661 acttccgggc ttaagccgta accgtcttcg aaacggtttg gaaccaggta gtcgatattg
    27721 ctgcaaccaa gcgagcgcat cgccagcacg cttagagccg tgctggtggc accgtcggcg
    27781 tcgaaatcac cgaccacaat aatccgcgtt ccttcgcgaa aggcgttgta aaggatctca
    27841 acggcctttt cgacgccgct cagttgctgc cagggcagca tccctttaac actgcgttcc
    27901 agttcttgcg cactgcgcac gccccggctg gcgtataaac ggcgcagcaa gggaggcaat
    27961 tcagcgggca agtctgccgt ttcatcgact gcacggcgac gaagttgtat ctgttgtttc
    28021 acgcgaatta tttaccgctg gtcatttttt ggtgttcgtc gaggaattct ttcatctctt
    28081 tcggcggctg gtaacccgga acaagtgtgc cattgctcag cacaactgcc ggagtaccgc
    28141 taacgccaag ctggacgcca agtgcgtaat ggtcggcaat atccacgtcg cagctggctg
    28201 gtgcgacgct tttacctgcc atcacatcat caaacgcttt gtttttatct ttcgcacacc
    28261 agatagcttt catttctttc tctgcatcgc tgtccagccc ctggcgcggg aaagcaagat
    28321 aacgcacggt gatccccagc gcgttatagt ctgccatttg ctcatgcagt ttgtggcagt
    28381 aaccacaggt aatatcagta aacacggtga tgacgtgttt ttcctgcagc gctttataaa
    28441 cgatcatctc tttttcaagc gcattcaact gctttaacag catcttattg gtgacattga
    28501 ccggagccgt gccgctaacg tcatacattg gcccctgaat gatatgttta ccatcatcgg
    28561 tgatgtacaa cacgccgctg ttagtcagaa ctgtcttcat gccagctacg ggcgcgggct
    28621 gaatatcgct gcttttgatg cccattttgg ctaacgtttg ttgaattgcc gcgtcatcag
    28681 cctgagcaaa acctgaaaac gccgctaaca aagtaaacaa cataaaacct ttcttcataa
    28741 atcttcccgt tcttttcaga catcacgccc gcgggtgatg ctgttgatga agttgccgca
    28801 gacgctcggt agcgacatgc gtataaattt gcgtggtgga aagatcgctg tggcccagta
    28861 gcatctgcac cacgcgtaaa tccgcaccat gatttaataa atgagtggca aaagcgtgac
    28921 gcaacacatg cggtgacagc ttttcgctgt cgatacccgc cagcacggca taatgtttaa
    28981 tgcggtgcca gaaggtctgt cgcgtcatct gctgcgcacg ctggctggga aacaacacgt
    29041 caattgacac accattcagc agccacggac gcccatgttc cagataggtt tccagccagt
    29101 aaaccgcctc ttcacctaac ggtaccaggc gctctttgtt gcctttacca atgacccgca
    29161 ctacgccctg acgcaggctg atatcactca ttgtcagccc gaccagttca gagacacgca
    29221 gcccggtagc atacaacact tcaagcatgg ctttatcgcg tagctccagt ggctgatcaa
    29281 ttaatggcgc ctgtaataaa cgttcgacct gcgcttcgct taaatctttt ggcaaacgct
    29341 ggggcaattt cggtgaagcg agatgcgcac tgggatcgtc ttcacgaaac ttttcgcgat
    29401 aaagatactg gaacaatcgg cgcactgcac tcagcaaacg cgctgagctg gtggctttat
    29461 acccgccctc cagccgttct gccagtaatg cctgcaaatc gtcactttgc gccgtcgcca
    29521 gcgtcaaccc gcggtgatgc aaccactcca ccatcattga cagatcgcga cggtaagcgt
    29581 tcaacgtatt ttcagccaga tttttttcca gccacagagc atcaagaaac tgctcgatgc
    29641 gtgccagatc ctgtttcact tgcgcccctt atggtcactc atttgatcca ttatgcctta
    29701 ttgtgccgtg actaaagcga ttctgataca ctagctgcaa aagccacagc agaatcgaga
    29761 agcttacgtt atgaatatgg gtctttttta cggttccagc acctgttaca ccgaaatggc
    29821 ggcagaaaaa atccgcgata ttatcggccc agaactggtg accttacata acctcaagga
    29881 cgactccccg aaattaatgg agcagtacga tgtgctcatt ctgggtatcc cgacctggga
    29941 ttttggtgaa atccaggaag actgggaagc cgtctgggat cagctcgacg acctgaacct
    30001 tgaaggtaaa attgttgcgc tgtatgggct tggcgatcaa ctgggatacg gcgagtggtt
    30061 cctcgatgcg ctcggtatgc tgcatgacaa actctcgacc aaaggcgtga agttcgtcgg
    30121 ctactggcca acggaaggat atgaatttac cagcccgaaa ccggtgattg ctgacgggca
    30181 actgttcgtg ggtctggcgc tggatgaaac taaccagtat gaccttagcg acgagcgtat
    30241 tcagagctgg tgcgagcaaa tcctcaacga aatggcagag cattacgcct gatctcactg
    30301 acggcttagc gcatatgctt tgccgtcatc tttatctttg cgtctcttgt tgcaacaaaa
    30361 tccgccgtaa atcccgccat tcggcttcgt ccatgctgtc ggctgccagc cataaatgtt
    30421 gccgtttacc gccatcagaa cgtaaacgca gcatcatgcc gctcttaatc atccacggtg
    30481 ctttgacgat gctccactcc tgcccttgcc aacgcaaacg cccgtccatc aacaagcgaa
    30541 tttccccctg gcgagcatta atacgccgct ggctgcgaac gcaatcaaac accaccagcg
    30601 aaagtaacac catccataac ggggtgtaac tgagtggcca gggcatgagt aaaataacag
    30661 cggcaaccag cccatgaatc agcaaggaaa gccactgtgc gcgccaggag acgcgcaaat
    30721 cagattgcca caggaccacg ttcccggttc cgtgtctgga tgagtcggac catcatttcc
    30781 agttctgcat cggccggttt accgtgattc atcagccagt taaacaggtc cggatcgtca
    30841 cattccagca gacgaataaa gatgcgtttt tcgtcatcgc ttaagctgtc gtactcatgt
    30901 tcgaaaaacg gcatgattga aatatcgagt tcgcgcatac cacggcggca tgcccaatga
    30961 atgcgggctt tgttgttaat gtccatcttc ttcctgtctc acgaaaatcc agtaccccgc
    31021 tattgtaacg tgtttttcgc cttcttttac gggaatatca gtaaacacca tcgcgatcac
    31081 gaaattaatt cacaacaatt caatggcttc atttttttgg aagtcgcctc gcagaaggca
    31141 cagatcgcgt agtgaaagca cttgcattgc ctcatagctc ttttaccatt agtcattaat
    31201 acgccgttaa gcaactcagg actctattat ggcttttaca ccttttcctc cccgtcagcc
    31261 tacggcttct gcccgtttac cgctgacgct gatgacgctt gatgactggg cgcttgccac
    31321 cattactggc gcggacagcg aaaaatatat gcagggtcag gtgacagcag atgtcagcca
    31381 gatggcagaa gatcagcacc tgctcgccgc ccattgcgac gccaaaggca aaatgtggag
    31441 caatttacgt ctgttccgcg acggcgatgg ctttgcgtgg attgaacggc gcagcgtgcg
    31501 tgaaccgcag ctgactgaac tgaaaaaata tgcggtgttc tctaaagtga ccatcgcgcc
    31561 agacgacgag catgtgctgc ttggtgttgc cggttttcag gcgcgcgccg cgctggcaaa
    31621 tatctttagt gaactgcctt cgaaagaaaa acaggtagtt aaagaaggcg cgaccacttt
    31681 gctatggttt gaatacccgg cagaacgttt cctgatcgta accgatgaag ctactgctaa
    31741 tatgctgacc gataaactgc gcggtgaagc ggaactgaac aatagccaac agtggctggc
    31801 attaaacatt gaagcgggtt tcccggtgat tgatgccgcc aacagcgggc agtttatccc
    31861 acaggcgacc aatctccagg cgctgggcgg tatcagcttt aagaaaggct gttataccgg
    31921 acaagagatg gtggcgcgag caaaattccg tggtgccaat aaacgtgcgc tctggttgct
    31981 ggcaggtagc gccagccgac tgccggaagc tggtgaagac ttagagctga aaatgggcga
    32041 gaactggcgt cgtaccggta cggtgctggc tgcggtaaaa ctggaagatg gtcaggtcgt
    32101 ggtacaggtc gtcatgaata acgatatgga accggatagc atcttccgcg tacgcgacga
    32161 tgcgaataca ttgcatattg agccgctgcc gtattcgctc gaagagtaaa tctctttatc
    32221 gcatcaggca tttatcgcct gatgcgacgc tgacgcgtct tatcatgtct gggatttgat
    32281 gcctttatca caacagggac gaggcccgta gatcggataa ggcgttcacg ccgaatccgg
    32341 cagttgtgct ccgatgcctg atgcgacgct gacgcgtctt ttcaggccta caaaggcata
    32401 cccattacgc ctgcccaata tacaaataga tcgccagaaa gtggcacaca ctgccgccga
    32461 gcacgaagcc gtgccagatg gcatgattgt agggaatgcg tttgcagaca tagaaaatca
    32521 ctccgagcga ataaaccacg ccgcctaccg ccaataaggt aacgctgccc gccgcgagct
    32581 taactgccat ttcataaatt accaccagcg acagccagcc catcgccaga taggtcacca
    32641 gagataaaat tttgaatcgg tgcgcgatgg tcagtttaaa cagaataccc agcaatgcca
    32701 ggctccagat aacaatcatc aacccgcgcg ctaacggaga atccagcccc accagcaaaa
    32761 acggcgtgta ggttccggca atcaacaggt aaatagcgca atggtcaaat ttcttcagcc
    32821 acatttttgc ccgttgatga ggaatggcgt gatagagcgt cgaagcgagg aacagcagga
    32881 tcatactgcc gccatagagg ctgtaactgg ttatcgctgt ggcgctggca ttgagatcca
    32941 ccgcctgaac cagtagcaac accagcccaa cgataccaaa caccaaccca atgccgtgac
    33001 tgacgctgtt ggcaatttcc tctgccagcg aatatccctg cttaatgagg ggcttctgaa
    33061 ccataactta ctccggagaa acgtacacgc acatgtatac ctctccagcg taactgagaa
    33121 tagttccagt gaacacctgt tagctaaaat aaattctgat ttgagactaa tctcctaaaa
    33181 atcatgaaat taaatgcgaa atttcaacta acaggcgtga gttcaattta aagacattta
    33241 aattcaatca cataaaactg tgtctgaccg ggatagatgt cggcaatgac ctttatcagt
    33301 tcagtcaggg tcatattttc ctgctctgca tgtttttccg tcagcgtatc cagcgttacg
    33361 gttgaggttg cggtgacttc aatcgtgcaa aaataaccgt catcttcaaa acgcccgaca
    33421 cgaagcacat cacccgtttt gaagtgcgat tcagactcgt cgcggatggt gatggtttta
    33481 cgcccagcca gaatgtcatc ctggaaacgt tgaaaaaaag tgatgtcgtt tggctgcatg
    33541 gtactatttc ctgtaagaat tgactcatct ggagcctatg atagtgaaaa aactcacctt
    33601 acagaaagat ttcttatggg gcggcgcagt tgccgctcat caggtcgaag gcggctggaa
    33661 caaaggcggc aaagggccga gcatttgtga cgttctgacc ggtggcgcac acggcgtgcc
    33721 gcgcgaaatc accaaagaag tcttgccagg caaatactat ccaaaccatg aagccgttga
    33781 tttttatggt cactacaagg aagacatcaa gctatttgcc gaaatgggct tcaaatgttt
    33841 tcggacttcc atagcctgga cgcgcatttt tccaaaaggc gatgaagctc agccaaacga
    33901 agaagggctg aagttctacg atgatatgtt cgatgaactg ctgaaataca acatcgaacc
    33961 ggtgatcacc ctctcccact ttgaaatgcc gctgcatctg gtgcagcaat acggtagctg
    34021 gaccaaccgt aaagtggttg atttctttgt ccgtttcgcg gaagtggtgt ttgaacgcta
    34081 taagcataaa gtcaaatact ggatgacctt caacgaaatt aacaaccagc gtaactggcg
    34141 tgcaccgctg ttcggttact gctgctccgg cgtggtttat accgagcatg agaacccgga
    34201 agagacgatg tatcaggtgc tgcatcacca gtttgtcgcc agcgccctgg cggtgaaagc
    34261 tgcgcgtcgc attaacccgg agatgaaagt cggctgtatg ctggcgatgg tgccgctcta
    34321 tccctactgc tgtaacccgg acgatgtgat gttcgctcag gagtcgatgc gcgaacgcta
    34381 cgtctttacc gatgtgcagc tacgcggcta ttacccgtcc tatgtgttga acgagtggga
    34441 gcgtcgcgga tttaacatca aaatggaaga cggcgatctc gatgtactgc gcgaaggcac
    34501 ctgcgattat cttggtttca gctattacat gaccaacgca gtgaaggccg aaggcggcac
    34561 cggcgatgcg atctctggtt tcgaaggcag cgtaccgaac ccgtatgtta aagcgtctga
    34621 ctggggctgg cagattgatc cagtaggtct gcgctatgcc ctttgcgaac tgtatgagcg
    34681 ttatcagagg ccgctgttta ttgtcgaaaa cggttttggc gcttacgaca aagtggaaga
    34741 agatggcagc atcaacgacg actaccgcat tgactacctg cgcgcccata tcgaagagat
    34801 gaaaaaagcg gtgacttacg atggcgtgga tctgatgggc tacacaccgt ggggctgcat
    34861 cgactgcgta tcgttcacca ccgggcagta cagcaaacgc tacggcttta tctatgtgaa
    34921 taaacatgac gacggtactg gcgatatgtc gcgttcacgt aagaagagct ttaactggta
    34981 caaagaggtg attaccagca acggcgagaa gctttaagtc gatgaagtac cggatgcaat
    35041 acttgttgca tccggtcaga caacttccct gttttatttc ccgcccgcca aatcgataaa
    35101 acttcccgtg acgtaagagg ctttatcact tagtagccag acaatggcct gcgcgacctc
    35161 ttctgcctgt ccaccacgct gcatggggat gttcgactta acgcgatcga cgcgtccagg
    35221 ctcgccgccg ctggcgtgca tttcggtaaa aataaaccct ggccgcacgc agttaacgcg
    35281 gaccccctgc gcggcgactt ccagcgatag tccggtggtt aacgtatcaa tcgccccttt
    35341 cgatgccgcg taatcaacat attcccctgg cgaacccaac cgtgaggcca ccgaagagac
    35401 attgacgata gcgccgccac tgccaccatt tttaagcgcc atgcgtttta ccgcctcgcg
    35461 gcagcagaga aaatatcccg tcatgttggt ggaaagtact cggttgattc gctctgcggt
    35521 aaggttttca acggtgcact gggtaaacaa gatcccggcg ttattgacca gcgctgctag
    35581 cggttcatcg tgctgatcga ttgctgtaaa cattgcgatg acctgatttt cgtcgctgat
    35641 atccgcctgg agcacgaatg ccttgccacc ggcttgcgtg attaagttca ccacttcctg
    35701 cgccgcatgg agattttgtt gataattaac cgccaccgta tactcttctt tcgccaacag
    35761 taatgcagtt gcccgcccga tgccgcgact gccatcggtc acaagtgcta tagccatttg
    35821 tttttccgag aaaaattaat tcgttgacgt atctttgaga ttgctaacaa actggctcaa
    35881 tcttcaatgt tggcacgctt ttcccctcgc cctttcaggg agagggccgg ggtgagggta
    35941 aatattcgcg ccagtgccgg cctgttcccc tcaccctaac cctctcccca aaggggcgag
    36001 gggactgtct gagcactttt gtactttgtc atctgactaa aaaggcgccg aagcgccttt
    36061 agaaaatagt cgaatcagtg aattactggt attcgctaat cggtacgcag gagcagaaca
    36121 ggttacggtc gccgtaaaca tcatccagac gtttcaccgt cggccagtat ttgtctgcca
    36181 cgcctgccgg gaataccgca acttcacggc tgtacggatg cgcccactcg gcgaccagtt
    36241 cgctctgaat gtgcggcgcg ttcaccagcg ggttatcttc cagcggccag acagcggctt
    36301 tcacctggtc aatttctgcg cggatagcca gcatcgcgtc gataaagcga tccagttcca
    36361 ctttgctttc agattcagtc ggttcaacca tcagcgtacc cgccaccggg aacgacatcg
    36421 tcggcgcgtg gaaaccgtag tcgatcaggc gcttggcaat atccagctcg ctgatgccgg
    36481 tttcttcttt cagcgggcga atatcgagaa tacattcgtg cgccacgcga ccgtcgcgac
    36541 cggtatacag caccgggaag gcatcctgca ggcggctggc aatatagttg gcgttgagga
    36601 ttgccacctg gcttgctttt ttcagccctt ctgcgcccat catgcggatg tacatccagc
    36661 tgattggcag gatagaggcg ctaccgaacg gtgccgcaga aactgcgccc tgacgggtta
    36721 acatgccttc gatttgcacc acgctatgac ccggtacaaa cggtgccaga tgcgctttca
    36781 caccgatcgg tcccataccc ggaccaccac cgccgtgcgg aatgcagaaa gttttatgca
    36841 ggttgaggtg cgaaacgtcc gcgccaataa agcccggcga ggtgatgcca acctgggcgt
    36901 tcatgttcgc gccatcaagg taaacctgac cgccgaactg atgcacgact tcacacactt
    36961 cacggatcgt ttcttcatac acgccgtggg tagaaggata agtcaccatg atacaggaga
    37021 ggttatcgcc cgcctgttcc gctttcgcgc gcagatcagt cagatcgatg ttgccgtttt
    37081 tatcacacgc cacaaccacc acctgcattc ctgccatatg tgcagaagcg gggttagttc
    37141 cgtgcgcaga agccgggatc aggcagatat cgcgatgccc ttcgttgcgg ctttcatgat
    37201 aatgacgaat cgccagcagg cccgcgtatt cgccctgtgc gccggagttc ggctgcatac
    37261 aaacggcgtc gtaaccggtc agtttcacca gccagtcagc cagctgcgca atcatctgct
    37321 gataaccttc agcctgttcc ggcgggcaaa acgggtgcag ttcggcaaat tccggccagg
    37381 tgattgggat catctcggcg gcggcgttca gtttcatggt gcaggaaccc agcgggatca
    37441 tcgcctgatt cagcgccaga tctttacgct ccagcgagtg catatagcgc atcatttcgg
    37501 tttcgctgtg gtagcgatta aacaccggat gggtgaggat ttcgtcgtcg cgcagcatcg
    37561 caggctggat agagcggctg tcgtgagcca cgtctttgtc cagcgtgtcg atctccagcc
    37621 cgtggttatc gcccagcagc acgctgaaaa gctgcattac gttttcacgc gtggtggttt
    37681 catcaagggt gatccccacg gcgttcagga tatcgctacg caggttgatt tcagccgctt
    37741 cggcacgcgt cagtacgccc gctttgtcgg ccacttccac acacaaggtg tcgaaatagt
    37801 gcgcatggcg cagtttcaga cctttttgtt gcaggcccgc cgccagaata tcggtcagac
    37861 ggtgaatgcg gttagcgata cgtttcaggc caaccgggcc gtgataaacg gcatacaggc
    37921 tggcgatgtt tgccagcagt acctgggaag tacaaatgtt ggagttcgct ttctcacgac
    37981 gaatatgttg ctcgcgagtc tgcatcgcca tgcgcagcgc ggtattgcca gctgcatctt
    38041 tcgatacacc gataatacgg cccggcattg agcgtttgta ttcatctttc gccgcaaaga
    38101 atgccgcgtg tgggccaccg tagcccatcg gcacgccgaa gcgttgcgcc gaaccaaaaa
    38161 caatatccgc gccctgttta cccggcgcag ttaacagcac cagcgccata atatcggcgg
    38221 caacgctgac cacaattttg cgtgatttca gttcgctaat aagcgcagtg tagtcgtgaa
    38281 tttcaccggt agtgcctacc tgctgtaaca gcacgccgaa gacgtcctga tggtcgagca
    38341 ctttttgcgc gtcatcgaca atcacttcaa aaccaaaggt ttcggcacga gtacggacca
    38401 catccagcgt ttgcggatgc acatcggaag ccacgaagaa gcggttggca tttttcagtt
    38461 tgctgacgcg tttcgccatc gccattgctt cggcggcagc ggtggcctcg tccagaagag
    38521 aagcagaggc catatccagt ccagtcaaat ccagcgttac ctgctggaag ttgagcagtg
    38581 cttcaaggcg gccctgggag acttcaggtt gatacggagt gtacgcggta taccagcccg
    38641 gattttccag catgttacgc aggataaccg gcggtagctg cacggcggtg taacccatgc
    38701 cgatgtaaga cgtgaagcgt ttattgcgac tggcaatagc cttgagttct gccagtgcgg
    38761 cgtattcggt cgccggtgcg ccaacctgcg gcggggtcgc aagctgaata tctttcggta
    38821 caatctggcc ggtcagcgcg tttaacgatt gtgcgccaac ggcattcagc atttcttgct
    38881 gttgcgcggc gtccggtccg atatggcgtt caataaaagc gccgctgttt tcaagctggc
    38941 ttaacgtctg tgtcatgagc gatggttcct gaaacgtgca gtgaattgtg aacctctctc
    39001 cttacgaaga gagtgagggt gaggcataaa ttttcctcac cctgatcctc tcccgcagaa
    39061 gaggaataaa gccgttactc gtcttctaac aatgcttcgt atgcggtcgc atccagcagt
    39121 gattccagtt cgctttcatc gctggctttg attttaaaga tccagccacc tgcatacggt
    39181 tcgctgttca ccagttccgg ggaatcgctc agtgcgtcgt ttaccgccac gatttcaccg
    39241 cttactggcg cataaatgtc tgacgccgct tttactgatt cggcaaccgc gcagtcatcg
    39301 cccgcgctaa ccgttgcgcc cacttccggc aggtcaacaa acaccatatc gcctaacagc
    39361 tcctgagcat gttcggtaat accaacggtg taagtgccgt cggcttcttt acgcagccat
    39421 tcgtgttctt tgctgtattt cagttctgct ggtacgttgc tcatcaatca atctccaaaa
    39481 aagtaaatca cgcgacggct ttgccgttac gcacaaaaac aggttttgtc actttaaccg
    39541 acatttcacg gttgcgaatt tgcacaatcg ccgtttcgcc aataccttcc agcacgcgcg
    39601 ccagcgcaat gctgtaaccc agcgtcgggg agaaagtacc gctggtgata atgccttcat
    39661 gctggttgcc ctgcgcatcg gtaaagcgta ccggcagttc attacgcagc acgccttttt
    39721 cggtcatcac cagaccaacc agtttttctg taccatgctc acgctgcact tccagggctt
    39781 cacgaccgat aaagtcacga tctgccggtt cccaggcgat agtccagccc atgttggcgg
    39841 ctaaaggaga aatagtttcg tccatctcct gaccataaag attcatgccc gcttccagac
    39901 gcagcgtgtc acgcgcgccc aagccacatg gcttaacacc cgcttccacc agcgcacgcc
    39961 agaaatcggc cgctttttca ttgggcagcg caatttcata gcccgcttca ccggtataac
    40021 cagtggtggc aataaacaga tcgcccgcct gcacgccaaa gaacggtttc atcccttcca
    40081 ccgcctgacg ctgggcgtca ttaaacagtg tggcagcttt tgcctgcgca ttcggccctt
    40141 gcacggcaat catggaaagg tcatcacgaa cggtaatttc gatgccgaaa ggttcagcgt
    40201 gttgggtaat ccaggagagg tctttttcgc gggtggcgga gttaacaacg aggcggaaga
    40261 aatcttcagt aaagtagtag acgatgaggt catctatcac accgccagag gcattcaaca
    40321 tccccgagta aagggctttg ccgcttttgg tgagcttcgc cacatcgttc gccagcagat
    40381 aacgcagaaa ctcccgggtg cggctgccgc ggagatcgac gatggtcata tgtgacacat
    40441 caaacattcc ggcatcggta cgtaccgcat gatgttcgtc gatttgcgaa ccgtaatgca
    40501 gcggcatcat ccagccgtgg aaatccacca tgcgagcgcc gcaaagcgtg tgttgttcgt
    40561 acaaaggagt ctgttgtgcc atcttgtcct cattgaataa gcggggctga caacttttca
    40621 tggtgaaatt atcaccacga aacccagcat cggagccact cccggtcccc aacgcaatcg
    40681 ttctcttttg cctgaactta ccaccgaaac agactgttaa ccataaggta aaattgatca
    40741 tcacattagc ttatggttaa aaaacgccaa aatcgcgaca gaataaaaaa ccaaaaaaca
    40801 caccagtttc tatataaaga tgatgccatt agaaagctaa tttgaataag gcaatattaa
    40861 gagctaaaaa aatgtcaaaa aacactaaat caaaaaataa tgacattaga aaatataatg
    40921 cgaaaacgga ggtgaaatta gtttatttca aatgaggaaa atctcccggc gaaaaaaccg
    40981 ggagatgaaa gtgtgatggg tatcaaataa acagcagaag agaaattttt aacgcagcca
    41041 ttcaggcaaa tcgtttaatc ccattgcctg gcggataagt tgtggtttaa cgccaggaag
    41101 cgtgtcggcc agtttcaaac caatatcacg cagcagtttt ttcgccggat tggtaccgga
    41161 aaacagatcg cggaatccct gcataccagc cagcatcagc gccgcactgt gcttgcggct
    41221 acgctcatac cggcgcaggt aaatgtactg cccgatgtct ttcccctgac gatgcaaccg
    41281 tttcagttca gcaatcagct ctgcagcatc cataaagcca agatttactc cctgccccgc
    41341 cagcgggtga atggtatgcg cggcgtcgcc caccagcacc agacggtgcg cggcaaactg
    41401 gcgcgcataa cgccccgtca gtgggaacac ctgacgcgcg ctctcaacct tgcataagcc
    41461 caggcgatta tcaaaagcga tatttaacgc gcgattaaat tcatcttcac ttgcctgctg
    41521 catccgctgc gcttcctctg gcgacagtga ccagacaatt gagcaaagat gcggatcgct
    41581 aagcggtaaa aaggccagaa tgccttcgcc atggaaaacc tgccgcgcca ccgcatcatg
    41641 cggttcttcc gtgcgaatgg tcgctaccag cgcgtgatgc tgataatccc agaaagtcag
    41701 cggaatatct gctttgttgc gcaaccagga attagcaccg tccgcgccaa tcaccagacg
    41761 cgccgttaac atgctgccat ctttcagcgt caggaaagtt tcattttctc cccaggcgac
    41821 ctgctgtaat tctgcggggg ccaacagagt gatatctgac gactgctgcg ctttgttcca
    41881 cagcgcgtag tgaatcactg agttttcaac gatatgccca agatggctat agcccatgct
    41941 ttgatcgtca aacgaaatgt gaccaaaact gtctttgtcc cacacttcca taccgtgata
    42001 acaactggcc ctacgagaga gaatgtcctg ccagacgcca agacgggtga gtaatttttc
    42061 gctggcggca ttgatagccg aaacgcgcag ttgtggtggt gcatccgccg ccagaggttc
    42121 cggtacgcgg tgctccagta cggcaacgcg taagccgctc ccctgtaagc cgcaggcaac
    42181 cgccagcccc accatgccgc caccaacaat ggctacatca acactttgca ttgtttattc
    42241 cttaaaaccg ccttcaacgc gccacccaac cgagggtgcg ctgcgccagc acatcgcgtg
    42301 ccggggtgaa taattccatc gtcatcagtc cgatgttgcg cccgacaacc agcggtgccc
    42361 aacggttggc aaaaagatgt accaggctgt ccgtgacgcc gatggttgct tcgcgatcgc
    42421 tctgtcgacg ctgctgatag cggcacaata tgccgtaatc accgatgtct tctccgcgct
    42481 catgcgcctg agtcagggtt tccgcaaggc tcatgacatc tcgcataccg aggttaaacc
    42541 cttgcccggc aatcgggtgc agagtttgcg ccgcattgcc caccagcacg gtacgatggg
    42601 taatagctct ggcggcgcgg gttaacgcca gcggataagc actgcgttta ccggcgtggg
    42661 taatttttcc aagtcgccag ccaaaggccg actggagttc acggcaaaac ttctcgtcgc
    42721 tccacgacag cacctcttcg cgccgttcca gtggatgaca ccagaccagc gaacagcgtc
    42781 cgtcagacat cggcaacatc gccagcgggc catgttgcgt gaagcgttca aaagcgcggc
    42841 cttcgtgcgc aacggaagtc gcaacgttgg caatcacggc cagttgttcg taaggctcct
    42901 gctgccagtc aacgccgcac gcggtggcta acgctgaatg ggtgccatca gctgctacca
    42961 gcacgcggcc cgtcagcgtc tcgccactct ccagcgtcac ttcaacgtga ctctgagtac
    43021 gggcaacgtt agccacgcga tcagggcaat gcagcgttac gccaggtgct ttacgcagca
    43081 atgcaaacag ccgttgcccg acattgtgca attcgacaac ctgtcccagc gccgccagtt
    43141 ggtaatcttc tgcggcgagg gtgacaaatc cagcgtgacc acgatcgctg acatgcacgg
    43201 tagtgatggc ggttgcgtaa tccgccagag attgccagac gccgatgcgc gccagttgct
    43261 gacaggtccc cgcagccagc gcaatagctc gtccatcaaa gcccggatga gcatgtgact
    43321 ctggcgcggt cgcttcaatc aaatgtaccg gcagcgcccc gtgacttaac cgggaaatag
    43381 ccagcgccag cgtcgcgccc gccatgccgc caccgacgat gattacgctc attgctttct
    43441 cgcagctgcc atcaacgctt cgatttcttc cggctttttc accacgctgg cggtgaggtt
    43501 ttcgttgccg gtttcggtaa tcacaatgtc gtcttcaata cgaatgccga taccgcgata
    43561 ttgttctggc acatccgcat ccggcgcgat atacagacca ggctctacgg tcagtaccat
    43621 ccccggttcc agaatgcgcg agcgatcctg accataaacg ccaacgtcat ggacatccag
    43681 tcctaaccag tggctaaggc catgcataaa gaaaggacga tgggcgttct gagcgatcag
    43741 ttcatcaaca tcacctttca ggatgccgag ttttaccagg ccgctaacca tgatgcgcac
    43801 cacttcacca gtgacttcca gaatggaagt tcccggacga tacaggcgca ggctggtttc
    43861 gagagactcc agcacaatgt cgtagatttc acgctgggcc tgggtgaatt tgccgttgac
    43921 cgggaaggtg cgggtaatat cgcccgcgta acctttgtat tcacaacccg cgtcaatcaa
    43981 caccaggtca ccgtcgcgca gttcactctc gttttcggtg tagtgcagaa tgcagccgtt
    44041 ttcaccgctg ccgacaatgg tgttatagga cggatagcgc gcaccgtggc ggttaaattc
    44101 gtggtgaatt tcgccttcca gatggtactc gaacattccc ggacggcatt tttccatcgc
    44161 ccgtgtatgt gccatggcgg tgatttctcc cgcgcggcgg agtacggcaa tctcttctgg
    44221 cgatttgaac aggcgcattt catgaacaac aggacgccag tcgatcatcg ttgccggtgc
    44281 ggtgagattt tgccgcgaac ctttacgcag tttttccagc gcactgttca tgatttcatc
    44341 agcatatgca tattcgccct gggcatggta aaccacatcc aggccgttaa gtagttgata
    44401 aagttgctga ttgatttcgc tgaatgccag tgcgcggtca acgcccagtt tctctggcgc
    44461 ggcatcctgg cctaagcgac ggccaaacca gatctccgcc gtcaggtcgc gaacgcggtt
    44521 aaacagaacg ctgtggttat gagtgtcatc gcttttaatc agcaccagca ccgcttccga
    44581 ttcgttaaag ccggtgaagt accagaagtc actgttctga cgataggggt attcgctgtc
    44641 ggcgctacgt gttacttctg gtgcagcaaa aatcagcgcg gcgctgccgg gttgcatttg
    44701 ctccaccagg gcctgacggc gacgctgaaa ctcttgccgg gatatctcac tcataacact
    44761 ctccttacgt tttttgtttt tagtgtagag tcggtttttg tacttctggc gcggtcggtt
    44821 gcggatgagt aaaggtgtcg tggcataaca gcgcggcaac acgaacgtat tcgatgatct
    44881 cttcaagcga catttcaagc tcttcctgat cttcgtcttc gtcgtaaccc agttgcgcaa
    44941 tgttacgcag atcgtcgata gcttcaccgg tttcgccggt cactttatcc agcttcggtt
    45001 gcgtaacgcc aagaccaagc aggaagtgat tgacccaacc tgccaatgca tcagcccgat
    45061 cgaaaacgct gacatcatcg ccatcaggca gataaagctg aaaaaggaag ccgtcatcct
    45121 gcagggcatc gctggtggca gagtgcattt tacgcagtgc ctgtgccagc tcatgaccga
    45181 aagccatgcc ttcgttcgtc aggtcgtgaa gtagcggcag ccatgagctg tcatcgttac
    45241 cgccacatat catcccactg attaaaccat gcatctcggc aggggtcagc cccgtccctt
    45301 gttggttcag atactggttc atttcgttgt aaccaggcat ttcgttctgt atagacataa
    45361 gcattcgtcg tcaaagggag gaatattcat gatatgctac cactttgggc cctggtggac
    45421 cagaaaaggg cttgtctctt ctcatcaggg tagctatagt gtcgcccctt cgcagaccat
    45481 gggtctaaag acgaaggcag cgcagtcaat cagcaggaag gtggcatgtc tgcacaaccc
    45541 gtcgatatcc aaatttttgg ccgttcactg cgtgtgaact gcccgcctga ccaaagggat
    45601 gcgttgaatc aggcagcgga cgatctgaac caacggttgc aagatctgaa agaacgcact
    45661 agagtcacaa atactgaaca gttggtcttc attgccgcat tgaatatcag ctatgagtta
    45721 gcgcaagaaa aagcgaagac tcgtgactac gcggcaagta tggaacagcg tattcggatg
    45781 ctgcagcaga ccatagaaca agcgttactt gaacaaggtc gcatcaccga aaaaactaac
    45841 caaaactttg aatgacactt ttcggtttac tgtggtagag taaccgtgaa gacaaaattt
    45901 ctctgagatg ttcgcaagcg ggccagtccc ctgagccgat atttcatacc acaagaatgt
    45961 ggcgctccgc ggttggtgag catgctcggt ccgtccgaga agccttaaaa ctgcgacgac
    46021 acattcacct tgaaccaagg gttcaagggt tacagcctgc ggcggcatct cggagattcc
    46081 cttcttatct ggcaccagcc atgacgcaac taccagaact cccactgaca ttatcccgac
    46141 aagaaatccg caaaatgatt cggcaacgtc gtcgtgcgtt aacgccggaa caacagcagg
    46201 aaatgggtca acaagccgct acccggatga tgacttatcc cccggtggtg atggcgcata
    46261 cggtcgctgt attcctctct tttgatggtg aactcgacac ccagccgcta atagaacaac
    46321 tctggcgcgc cggtaagcgc gtatatcttc cagttttgca tccctttagt gccggtaatt
    46381 tgctgttcct gaattaccat ccgcaaagcg aactggtgat gaacaggttg aagatccatg
    46441 agccaaaact ggatgtgcgt gacgtgctac ccctttcccg attagacgtg ctgatcacac
    46501 cgctggtcgc ctttgatgag tacggtcagc gcctgggaat gggcggtggt ttttatgatc
    46561 ggaccttaca aaactggcag cactataaaa cgcaaccggt gggttatgcg catgattgtc
    46621 agttggtgga aaaactcccc gttgaagagt gggatatccc tcttcctgcg gtggttacac
    46681 cgtcgaaagt ctgggagtgg taagggcgat acacctgcat cgccctgatt gacatcgttg
    46741 attctttaac ctaatttagt gagtaagggt aagggaggat tgctcctccc ctgtgactga
    46801 ctgttaataa gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat
    46861 cataaccctt ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc
    46921 tgcccagcta tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct
    46981 tttcttcgag cgtgctcgaa aaacccatgc aaaacagaga taatttttgg gaaaaaggat
    47041 ctattacggc agtaaacgtg ggcgatgaag tggctatcaa aaagttcggt tcagggcgat
    47101 gcaccccgca tcgccctgat tgacatcgtt gattctttga cctaatttag tgagtaaggg
    47161 taagagagga tgactcctcc cctgagactg actgttaata agctccgaaa cttatcagta
    47221 acagtacaat cagtatgatg acaagtcgca tcataaccct tctcctttaa gccctcgctt
    47281 cggtgagggc tttaccgtta cagccccatg ctgtcccgcc atcgtaaatc cccattaaat
    47341 aaacacaacg cattgatctg agtttgattt attttctgga gcagactcgc aaagtagaat
    47401 gcgcaacgcg gcaacggtgt ggagaaggga taaaaaaacg ggcaagtcag tgacctgccc
    47461 gttaattttc agagaagggg aattagtaca gcagacgggc gcgaatggta cccggaatag
    47521 ctttcattgc ctgcagcgct ttttcggcaa cgtcttcgtc agcttcaata tcaataacta
    47581 cataacccat ctgggcggaa gtttgtagat attgcgcggc gatgttgacg ccctgctcgg
    47641 cgaagatttt gttcagcgca gttagcacgc ccggacggtt ttcgtggatg tgcatcagac
    47701 gacgaccacc atgcagtggc agcgagactt ccgggaagtt caccgcagag agcgttgagc
    47761 cattgtcaga atacttgatc aatttacccg caacttccag gccgatattc tcctgcgctt
    47821 cctgagtcga accgccaatg tgtggcgtca gaaggacgtt gtcgaattca cacagtggag
    47881 aggtaaatgg atcgctattg gtcgccggtt ccgtcgggaa tacgtcgatt gccgcccccg
    47941 ccagatgttt gctcgccagc gcatcacaca gcgccggaat atccaccaca gtaccgcgcg
    48001 aggcattaat cagcagcgag ccgggcttca ttagcgaaat ctctttcgcg cccatcatat
    48061 ttttggtgga cggattctct ggtacatgca gactcaccac atcgctcata ttcagcaggt
    48121 cagaaagatg ctgtacctga gtggcgttgc ccagcggcag tttgttttca atatcataaa
    48181 agtaaacata cattcccagc gattcagcca gaatgcccaa ttgcgtacca atatgaccgt
    48241 agccgatgat acccagcttt ttgccgcgcg cttcaaaaga acccgccgcc agtttgttcc
    48301 acacgccacg gtgcgcttta gcattggctt ccggcacgcc gcgcaatagc agcagcagtt
    48361 cgccaatcac cagctccgca acagagcgcg tatttgagaa cggtgcgtta aataccggga
    48421 tcccgcgctt tgccgccgca tccagatcaa cctggtttgt tccgatacag aaacagccaa
    48481 tagcgaccag tttttctgcg gcgttgatca cgtcttcagt cagatgggta cgggatcgca
    48541 ggccgatgaa gtgggcatcg cggatggatt cttttaattg ttcatcatcc agcgcgcctt
    48601 tgtgaaattc gatgttggtg taaccagctg cacgaaggct ttccagcgcc ttttggtgca
    48661 cgccttctac cagcagaaac ttaatcttgt ctttctccag cgataccttt gccatttacc
    48721 caatcctgtc ttttgaaatg ttgtgtgcgg atttgcatcc gcctttcaac atatcaaaaa
    48781 ataatattgc ggcaatatga acgtttgcgt cgcgatgttg aggaaatatc acgcaaaggt
    48841 atttcttggt tgaatatgct ggtttagagg atttatttag agcaatcgac aatagccaag
    48901 taaaaacgtg acacatgtca ccaaatttaa tgaagagaat ttttttaacg ggggaggttc
    48961 ccccgtcaga tcatttcaca atggttttga caccgtcagg tgtgccaatc agcgcaacgt
    49021 ccgcgccacg gttagcaaac aagccaacag tcaccacgcc aggaatcgca tttatggcgt
    49081 tttccatcgc tatcgggtca aggatttcca tgccgtggac gtcgaggatc acgttgccgt
    49141 tatcggtcac cacgccctga cggtattccg gacgaccgcc cagtttcacc agctggcgcg
    49201 ccactgcact acgtgccatc gggataactt ctactggcag cgggaatttg cccagaatat
    49261 caacctgctt ggaagcgtca gcaatacaga taaatttttc cgcaaccgaa gcaatgattt
    49321 tttcacgggt cagcgccgcg ccgccgcctt tgatcatttg catgtggccg ttgatttcat
    49381 ctgcgccatc aacgtagatg ccaaggctgt cgacttcgtt gagatcaaaa acgtgaatgc
    49441 cgaggctttt cagtttttca gtggaagcat ctgaactgga aacggcccct tcaatctggc
    49501 ctttcattgt accgagcgcg tcaataaagt gtgcggcggt ggaacctgta cctacaccaa
    49561 caatggtgcc gggctgaaca tactgaagtg ccgcccatcc tactgctttt ttcaattcat
    49621 cctgcgtcat gatcgtttcg cctgtggtat gaaatttcac acgcattata tacaaaaaaa
    49681 gcgattcaga ccccgttggc aagccgcgtg gttaactcat ccataaaata tcgcgcaatg
    49741 gcgggcatcc cctttcgccc cgcaaataaa gcatacaacg gtctgggtat gccgctccac
    49801 ggtgcgaaca ggcgcaccag ttccccccgt tcgcaagccc ctgtttacag gcaaattgag
    49861 gcaataacgc caccccattc aacacaacca gggcacgaac tcgctgagcg aaatgcatta
    49921 gcgcaaatcc ctgatctttt aagctgcgcg tttaaccacg gatacaaatg ttacccgccg
    49981 acggcttcgg tatatgcaac ctgacacaaa attgtgtcat agtgcaggaa aaagcattta
    50041 ccaggagcag acaacagcaa tgaaacgccc ggactacaga acattacagg cactggatgc
    50101 ggtgatacgt gaacgaggat ttgagcgcgc ggcacaaaag ctgtgcatta cacaatcagc
    50161 cgtctcacag cgcattaagc aactggaaaa tatgttcggg cagccgctgt tggtgcgtac
    50221 cgtaccgccg cgcccgacgg aacaagggca aaaactgctg gcactgctgc gccaggtgga
    50281 gttgctggaa gaagagtggc tgggcgatga acaaaccggt tcgactccgc tgctgctttc
    50341 actggcggtc aacgccgaca gtctggcgac gtggttgctt ccggcactgg ctcctgtgtt
    50401 ggctgattcg cctatccgcc tcaacttgca ggtagaagat gaaacccgta ctcaggaacg
    50461 tctgcgccgc ggcgaagtgg tcggcgcggt gagtattcaa catcaggcgc tgccgagttg
    50521 tcttgtcgat aaacttggtg cgctcgacta tctgttcgtc agctcaaaac cctttgccga
    50581 aaaatatttc cctaacggcg taacgcgttc ggcattactg aaagcacctg tggtcgcctt
    50641 tgaccatctt gacgatatgc accaggcctt tttgcagcaa aacttcgatc tgcctccagg
    50701 cagcgtgccc tgccatatcg ttaattcttc agaagcgttc gtgcaacttg ctcgccaggg
    50761 caccacctgc tgtatgatcc cgcacctgca aatcgagaaa gaactagcca gcggtgaact
    50821 gattgactta acgccagggc tatttcaacg ccggatgctc tactggcacc gctttgctcc
    50881 tgaaagccgc atgatgcgta aagtcactga tgcgttgctc gattatggtc acaaagtcct
    50941 tcgtcaggat taatccatca aataatgcct gatagcacat atcaggcgtt atcctcactt
    51001 ctttttgtat tccttgaatc acatcacaaa atagacaaat ctcaggcggc aaaaaacgac
    51061 gtctgaatgc atttttttgc tggcgacaaa cccacgtaaa aagctcaccg taagcgcaaa
    51121 taccctcatt ttgattgcgt tttacggagc aaataatgtc taacgagcag gagtggcaac
    51181 agcttgccaa caaggaattg agccgtcggg agaaaactgt cgactcgctg gttcagcaaa
    51241 ccgcggaagg gatcgccatc aagcagctgt ataccgaagc cgatctcgat aatctggagg
    51301 tgacaggtac ccttcctggt ttgccgccct acgttcgtgg cccgcgtgcc actatgtata
    51361 ccgcccaacc gtggaccatc cgtcagtatg ctggtttttc aacagcaaaa gagtccaacg
    51421 ctttttatcg ccgtaacctg gccgccgggc aaaaaggtct ttccgttgcg tttgaccttg
    51481 ccacccaccg tggctacgac tccgataacc cacgcgtggc gggtgacgtc ggcaaagcgg
    51541 gcgtcgctat cgacaccgtg gaagatatga aagtcctgtt cgaccagatc ccgctggata
    51601 aaatgtcggt ttcgatgacc atgaatggcg cagtgctacc agtactggcg ttttatatcg
    51661 tcgccgcaga agagcaaggt gttacacctg ataaactgac cggcaccatt caaaacgata
    51721 ttctcaaaga gtacctctgc cgcaacacct atatttaccc accaaaaccg tcaatgcgca
    51781 ttatcgccga catcatcgcc tggtgttccg gcaacatgcc gcgatttaat accatcagta
    51841 tcagcggtta ccacatgggg gaagcgggtg ccaactgcgt gcagcaggta gcatttacgc
    51901 tcgccgatgg gattgagtac atcaaagcag caatctctgc cggactgaaa attgatgact
    51961 tcgctcctcg cctgtcgttc ttcttcggta tcggcatgga tctgtttatg aacgtcgcca
    52021 tgttgcgtgc ggcacgttat ttatggagcg aagcggtcag tggatttggc gcacaggatc
    52081 cgaaatcact ggcgctgcgt acccactgcc agacctcagg ctggagcctg actgaacagg
    52141 atccgtataa caacgttatc cgcaccacca ttgaagcact ggctgcgacg ctgggcggta
    52201 ctcagtcact gcataccaac gcctttgacg aagcgcttgg tttgcctacc gatttctcag
    52261 cacgcattgc ccgcaacacc cagatcatca tccaggaaga atcagaactc tgccgcaccg
    52321 tcgatccact ggccggatcc tattacattg agtcgctgac cgatcaaatc gtcaaacaag
    52381 ccagagctat tatccaacag atcgacgaag ccggtggcat ggcgaaagcg atcgaagcag
    52441 gtctgccaaa acgaatgatc gaagaggcct cagcgcgcga acagtcgctg atcgaccaag
    52501 gcaagcgtgt catcgttggt gtcaacaagt acaaactgga tcacgaagac gaaaccgatg
    52561 tacttgagat cgacaacgtg atggtgcgta acgagcaaat tgcttcgctg gaacgcattc
    52621 gcgccacccg tgatgatgcc gccgtaaccg ccgcgttgaa cgccctgact cacgccgcac
    52681 agcataacga aaacctgctg gctgccgctg ttaatgccgc tcgcgttcgc gccaccctgg
    52741 gtgaaatttc cgatgcgctg gaagtcgctt tcgaccgtta tctggtgcca agccagtgtg
    52801 ttaccggcgt gattgcgcaa agctatcatc agtctgagaa atcggcctcc gagttcgatg
    52861 ccattgttgc gcaaacggag cagttccttg ccgacaatgg tcgtcgcccg cgcattctga
    52921 tcgctaagat gggccaggat ggacacgatc gcggcgcgaa agtgatcgcc agcgcctatt
    52981 ccgatctcgg tttcgacgta gatttaagcc cgatgttctc tacacctgaa gagatcgccc
    53041 gcctggccgt agaaaacgac gttcacgtag tgggcgcatc ctcactggct gccgatcata
    53101 aaacgctgat cccggaactg gtcgaagcgc tgaaaaaatg gggacgcgaa gatatctgcg
    53161 tggtcgcggg tggcgtcatt ccgccgcagg attacgcctt cctgcaagag cgcggcgtgg
    53221 cggcgattta tggtccaggt acacctatgc tcgacagtgt gcgcgacgta ctgaatctga
    53281 taagccagca tcatgattaa tgaagccacg ctggcagaaa gtattcgccg cttacgtcag
    53341 ggtgagcgtg ccacgctcgc ccaggccatg acgctggtgg aaagccgtca cccacgtcat
    53401 caggcgctaa gtacacagct gcttgacgcc attatgccgt tctgtggtaa cgccctgcga
    53461 ctgggcgtta ccggcacacc cggcgcgggg aaaagtacct ttcttgaagc ctttggcatg
    53521 ttgttgattc gtgagggatt aaaggttgct gttattgcgg tcgatcccag cagcccggtc
    53581 actggcggta gcattctcgg ggataaaacc cgcatgaatg acctggcgcg tgccgaggcg
    53641 gcgtttattc gcccggtacc ctcctccggt catctgggcg gtgccagtca gcgagcacgg
    53701 gaattaatgc tgttatgcga agcggcgggt tatgacgtag tgattgtcga aacggttggc
    53761 gtcgggcagt cggaaacgga agtcgcccgc atggtggact gttttatctc gttgcaaatt
    53821 gccggtggcg gcgatgatct gcagggcatt aaaaaagggc tgatggaagt ggctgatctg
    53881 ctcgttatca acaaagacga tggcgataac cataccaatg tcgccattgc ccggcatatg
    53941 tacgagagtg ccctgcatat tctgcgacgt aaatacgacg aatggcagcc acgggttctg
    54001 acttgtagcg cactggaaaa acgtggaatc gatgagatct ggcacgccat catcgacttc
    54061 aaaaccgcgc taactgccag tggtcgttta caacaagtgc ggcaacaaca atcggtggaa
    54121 tggctgcgta agcagaccga agaagaagta ctgaatcacc tgttcgcgaa tgaagatttc
    54181 gatcgctatt accgccagac gcttttagcg gtcaaaaaca atacgctctc accgcgcacc
    54241 ggcctgcggc agctcagtga atttatccag acgcaatatt ttgattaaag gaatttttat
    54301 gtcttatcag tatgtgaacg ttgccactat caacaaagtg gcggtcattg agtttaacta
    54361 tggacgaaaa cttaatgcct taagtaaagt ctttattgat gatcttatgc aggcgttaag
    54421 cgatctcaac cggccggaaa ttcgctgtat cattttgcgg gcgccaagtg gatccaaagt
    54481 cttctccgca ggtcacgata ttcacgaact gccgtctggc ggtcgcgatc cgctctccta
    54541 tgatgatcca ttgcgtcaaa tcacccgcat gatccaaaaa ttcccgaaac cgatcatttc
    54601 gatggtggaa ggtagtgttt ggggtggcgc atttgaaatg atcatgagtt ccgatctgat
    54661 catcgccgcc agtacctcaa ccttctcaat gatgcctgta aacctcggcg tcccgtataa
    54721 cctggtcggc attcacaacc tgacccgcga cgcgggcttc cacattgtca aagagctgat
    54781 ttttaccgct tcgccaatca ccgcccagcg cgcgttggct gtcggcatcc tcaaccatgt
    54841 tgtggaagtg gaagaactgg aagatttcac cttacaaatg gcgcaccaca tctctgagaa
    54901 agcgccgtta gccattgctg ttatcaaaga agagctgcgt gtactgggcg aagcgcacac
    54961 catgaactcc gatgaatttg aacgtattca ggggatgcgc cgcgcggttt atgacagcga
    55021 agattaccag gaagggatga acgctttcct cgaaaaacgt aaacctaatt tcgttggtca
    55081 ttaatccctg cgaacgaagg agtaaaaatg gaaactcagt ggacaaggat gaccgccgat
    55141 gaagcggcag aaattatcca gcataacgac atggtggcat ttagcggctt taccccagcg
    55201 ggttcgccga aagccttacc gaccgcgatt gcccgcagag ctaacgaaca gcatgaggcc
    55261 aaaaagccgt atcaaattcg cctgctgacg ggggcgtcaa tcagcgccgc cgctgacgat
    55321 gtactttctg acgccgatgc tgtttcctgg cgtgcgccat atcaaacatc gtccggttta
    55381 cgtaaaaaga tcaatcaggg cgcggtgagt ttcgttgacc tgcatttgag cgaagtggcg
    55441 caaatggtca attacggttt cttcggcgac attgatgttg ccgtcatcga agcatcggca
    55501 ctggcaccgg atggtcgagt ctggttaacc agcgggatcg gtaatgcgcc gacatggctg
    55561 ctgcgggcga agaaagtgat cattgaactt aatcactatc acgatccgcg cgttgcagaa
    55621 ctggcggata ttgtgattcc tggcgcacca ccgcggcgca atagcgtgtc gatcttccat
    55681 gcaatggatc gcgtcggtac ccgctatgtg caaatcgatc cgaaaaagat tgtcgccgtc
    55741 gtggaaacca acttgcccga cgccggtaat atgctggata agcaaaatcc catgtgccag
    55801 cagattgccg ataacgtggt cacgttctta ttgcaggaaa tggcgcatgg gcgtattccg
    55861 ccggaatttc tgccgctgca aagtggcgtg ggcaatatca ataatgcggt aatggcgcgt
    55921 ctgggggaaa acccggaaat tcctccgttc atgatgtatt cggaagtgct acaggaatcg
    55981 gtggtgcatt tactggaaac cggcaaaatc agcggggcca gcgcctccag cctgacaatc
    56041 tcggccgatt ccctgcgcaa gatttacgac aatatggatt actttgccag ccgcattgtg
    56101 ttgcgtccgc aggagatttc caataacccg gaaatcatcc gtcgtctggg cgtcatcgct
    56161 ctgaacgtcg gcctggagtt tgatatttac gggcatgcaa attcaacgca cgtagccggg
    56221 gtcgatctga tgaacggcat cggcggcagc ggtgattttg aacgcaacgc gtatctgtcg
    56281 atctttatgg ctccgtcgat tgctaaagaa ggcaagatct caaccgtcgt gccaatgtgc
    56341 agccatgttg atcacagcga acacagcgtc aaagtgatca tcaccgaaca agggatcgcc
    56401 gatctacgcg gtctttcccc gcttcaacgc gcccgcacta tcattgataa ttgtgcacat
    56461 cctatgtatc gggattatct gcatcgctat ctggaaaatg cgcccggcgg acatattcac
    56521 cacgatctta gccacgtctt cgacttacac cgtaatttaa ttgcaaccgg ctcgatgctg
    56581 ggttaatggt ttgtcccgtc acgatcttca gcaatatgct gaagcatcgt ggcggtataa
    56641 cgatgatttc tgattgaata aagatattcc tgcatataca tcggcctgtg cggcgcatca
    56701 aaatatttca gcgttgccgg attcaccagt cgccaggcaa aatgcgggat caccgtcaga
    56761 aaatgtccgc gctccacggc gctgattttg gccataaaac tgtagggacg ataaataatg
    56821 gtgggattga tcccacaagg tcgcatattc gcatcaatca ttgcttcgaa gttagcccgg
    56881 ttttgaaagc gcatttgtag ccagggcagc tcctgaagca gctcttgctc ttgacgctct
    56941 tcataacggc gagaaacgag aaagcccaat cgcagcggtg gtaattcact gatagtcagg
    57001 ttttccagtt cctgaactcg tgcagataca tgctgtgggg agataataaa atctaactga
    57061 cggtcaaaaa gattatcgat aacgccgttt tcgctgaact caactggctg ggcggtcacg
    57121 ccttcatact tatctccgag actaatcaac tgatcgaaaa taattgtcgg ataagtatta
    57181 tctatgccaa tcacaatt
//
LOCUS       DAZOYS010000005        54675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOYS010000005 DAZOYS010000000
VERSION     DAZOYS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.005"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            180..230
                     /gene="hisL"
                     /locus_tag="ACHUN9_000221"
     CDS             180..230
                     /gene="hisL"
                     /locus_tag="ACHUN9_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0693740.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    206..329
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            376..1275
                     /gene="hisG"
                     /locus_tag="ACHUN9_000222"
     CDS             376..1275
                     /gene="hisG"
                     /locus_tag="ACHUN9_000222"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0693741.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1281..2585
                     /gene="hisD"
                     /locus_tag="ACHUN9_000223"
     CDS             1281..2585
                     /gene="hisD"
                     /locus_tag="ACHUN9_000223"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0693742.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            2582..3652
                     /gene="hisC"
                     /locus_tag="ACHUN9_000224"
     CDS             2582..3652
                     /gene="hisC"
                     /locus_tag="ACHUN9_000224"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0693743.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3652..4719
                     /gene="hisB"
                     /locus_tag="ACHUN9_000225"
     CDS             3652..4719
                     /gene="hisB"
                     /locus_tag="ACHUN9_000225"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0693744.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4719..5309
                     /gene="hisH"
                     /locus_tag="ACHUN9_000226"
     CDS             4719..5309
                     /gene="hisH"
                     /locus_tag="ACHUN9_000226"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0693745.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5309..6046
                     /gene="hisA"
                     /locus_tag="ACHUN9_000227"
     CDS             5309..6046
                     /gene="hisA"
                     /locus_tag="ACHUN9_000227"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0693746.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6028..6804
                     /gene="hisF"
                     /locus_tag="ACHUN9_000228"
     CDS             6028..6804
                     /gene="hisF"
                     /locus_tag="ACHUN9_000228"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0693747.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6798..7409
                     /gene="hisIE"
                     /locus_tag="ACHUN9_000229"
     CDS             6798..7409
                     /gene="hisIE"
                     /locus_tag="ACHUN9_000229"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0693748.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(7506..8483)
                     /gene="wzzB"
                     /locus_tag="ACHUN9_000230"
     CDS             complement(7506..8483)
                     /gene="wzzB"
                     /locus_tag="ACHUN9_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0693749.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8629..9795)
                     /gene="ugd"
                     /locus_tag="ACHUN9_000231"
     CDS             complement(8629..9795)
                     /gene="ugd"
                     /locus_tag="ACHUN9_000231"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0693750.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10045..11451)
                     /gene="gndA"
                     /locus_tag="ACHUN9_000232"
     CDS             complement(10045..11451)
                     /gene="gndA"
                     /locus_tag="ACHUN9_000232"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0693751.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11615..12985)
                     /gene="cpsG"
                     /locus_tag="ACHUN9_000233"
                     /gene_synonym="manB"
     CDS             complement(11615..12985)
                     /gene="cpsG"
                     /locus_tag="ACHUN9_000233"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0693752.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(13090..14526)
                     /gene="cpsB"
                     /locus_tag="ACHUN9_000234"
     CDS             complement(13090..14526)
                     /gene="cpsB"
                     /locus_tag="ACHUN9_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0693753.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(14529..15752)
                     /gene="wcaI"
                     /locus_tag="ACHUN9_000235"
     CDS             complement(14529..15752)
                     /gene="wcaI"
                     /locus_tag="ACHUN9_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0693754.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(15749..16228)
                     /gene="gmm"
                     /locus_tag="ACHUN9_000236"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(15749..16228)
                     /gene="gmm"
                     /locus_tag="ACHUN9_000236"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0693755.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(16231..17196)
                     /gene="fcl"
                     /locus_tag="ACHUN9_000237"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(16231..17196)
                     /gene="fcl"
                     /locus_tag="ACHUN9_000237"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0693756.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(17199..18320)
                     /gene="gmd"
                     /locus_tag="ACHUN9_000238"
     CDS             complement(17199..18320)
                     /gene="gmd"
                     /locus_tag="ACHUN9_000238"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0693757.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(18346..18894)
                     /gene="wcaF"
                     /locus_tag="ACHUN9_000239"
     CDS             complement(18346..18894)
                     /gene="wcaF"
                     /locus_tag="ACHUN9_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0693758.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(18910..19656)
                     /gene="wcaE"
                     /locus_tag="ACHUN9_000240"
     CDS             complement(18910..19656)
                     /gene="wcaE"
                     /locus_tag="ACHUN9_000240"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0693759.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(19667..20884)
                     /gene="wcaD"
                     /locus_tag="ACHUN9_000241"
     CDS             complement(19667..20884)
                     /gene="wcaD"
                     /locus_tag="ACHUN9_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0693760.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(20859..22076)
                     /gene="wcaC"
                     /locus_tag="ACHUN9_000242"
     CDS             complement(20859..22076)
                     /gene="wcaC"
                     /locus_tag="ACHUN9_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0693761.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(22073..22561)
                     /gene="wcaB"
                     /locus_tag="ACHUN9_000243"
     CDS             complement(22073..22561)
                     /gene="wcaB"
                     /locus_tag="ACHUN9_000243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0693762.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(22564..23403)
                     /gene="wcaA"
                     /locus_tag="ACHUN9_000244"
     CDS             complement(22564..23403)
                     /gene="wcaA"
                     /locus_tag="ACHUN9_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0693763.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(23496..25658)
                     /gene="wzc"
                     /locus_tag="ACHUN9_000245"
     CDS             complement(23496..25658)
                     /gene="wzc"
                     /locus_tag="ACHUN9_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0693764.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(25661..26104)
                     /gene="wzb"
                     /locus_tag="ACHUN9_000246"
     CDS             complement(25661..26104)
                     /gene="wzb"
                     /locus_tag="ACHUN9_000246"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0693765.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(26110..27249)
                     /gene="wza"
                     /locus_tag="ACHUN9_000247"
     CDS             complement(26110..27249)
                     /gene="wza"
                     /locus_tag="ACHUN9_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0693766.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            27558..27707
                     /locus_tag="ACHUN9_000248"
     CDS             27558..27707
                     /locus_tag="ACHUN9_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693767.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            27908..29491
                     /locus_tag="ACHUN9_000249"
     CDS             27908..29491
                     /locus_tag="ACHUN9_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0693768.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(29765..31618)
                     /gene="asmA"
                     /locus_tag="ACHUN9_000250"
     CDS             complement(29765..31618)
                     /gene="asmA"
                     /locus_tag="ACHUN9_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0693769.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(31640..32221)
                     /gene="dcd"
                     /locus_tag="ACHUN9_000251"
     CDS             complement(31640..32221)
                     /gene="dcd"
                     /locus_tag="ACHUN9_000251"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0693770.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(32313..32954)
                     /gene="udk"
                     /locus_tag="ACHUN9_000252"
     CDS             complement(32313..32954)
                     /gene="udk"
                     /locus_tag="ACHUN9_000252"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0693771.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            33272..36589
                     /locus_tag="ACHUN9_000253"
     CDS             33272..36589
                     /locus_tag="ACHUN9_000253"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0693772.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(36699..37547)
                     /gene="alkA"
                     /locus_tag="ACHUN9_000254"
     CDS             complement(36699..37547)
                     /gene="alkA"
                     /locus_tag="ACHUN9_000254"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0693773.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            37681..39033
                     /gene="yegD"
                     /locus_tag="ACHUN9_000255"
     CDS             37681..39033
                     /gene="yegD"
                     /locus_tag="ACHUN9_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0693774.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(39046..40986)
                     /gene="yegI"
                     /locus_tag="ACHUN9_000256"
     CDS             complement(39046..40986)
                     /gene="yegI"
                     /locus_tag="ACHUN9_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0693775.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(40983..41744)
                     /gene="pphC"
                     /locus_tag="ACHUN9_000257"
     CDS             complement(40983..41744)
                     /gene="pphC"
                     /locus_tag="ACHUN9_000257"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0693776.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(41741..42400)
                     /locus_tag="ACHUN9_000258"
     CDS             complement(41741..42400)
                     /locus_tag="ACHUN9_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0693777.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(42621..42680)
                     /gene="ibsA"
                     /locus_tag="ACHUN9_000259"
     CDS             complement(42621..42680)
                     /gene="ibsA"
                     /locus_tag="ACHUN9_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0693778.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(42953..43009)
                     /locus_tag="ACHUN9_000260"
     CDS             complement(42953..43009)
                     /locus_tag="ACHUN9_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0693779.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(43280..43336)
                     /locus_tag="ACHUN9_000261"
     CDS             complement(43280..43336)
                     /locus_tag="ACHUN9_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0693780.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            43615..44856
                     /gene="mdtA"
                     /locus_tag="ACHUN9_000262"
     CDS             43615..44856
                     /gene="mdtA"
                     /locus_tag="ACHUN9_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0693781.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            44856..47978
                     /gene="mdtB"
                     /locus_tag="ACHUN9_000263"
     CDS             44856..47978
                     /gene="mdtB"
                     /locus_tag="ACHUN9_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0693782.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            47979..51056
                     /gene="mdtC"
                     /locus_tag="ACHUN9_000264"
     CDS             47979..51056
                     /gene="mdtC"
                     /locus_tag="ACHUN9_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0693783.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            51057..52472
                     /locus_tag="ACHUN9_000265"
     CDS             51057..52472
                     /locus_tag="ACHUN9_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0693784.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            52469..53872
                     /gene="baeS"
                     /locus_tag="ACHUN9_000266"
     CDS             52469..53872
                     /gene="baeS"
                     /locus_tag="ACHUN9_000266"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0693785.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            53869..54591
                     /gene="baeR"
                     /locus_tag="ACHUN9_000267"
     CDS             53869..54591
                     /gene="baeR"
                     /locus_tag="ACHUN9_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0693786.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        12709 a        14577 c        14220 g        13169 t
ORIGIN      
        1 aggcttcgct atttttggtg ccgtcagctt agaggacagt cctcttagcc ccctcctttc
       61 cccgctcatt cattaaacaa atccattgcc ataaaatata taaaaaagcc cttgctttct
      121 aacgtgaaag tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta
      181 tgacacgcgt tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc
      241 gatgtgtgct ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc
      301 ggaagatcac cttccggggg cttttttatt gcgcggttga taacggttca gacaggttta
      361 aagaggaata acaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc
      421 cgtttaagtg atgactcacg cgaattactg gcgcgctgtg gcattaaaat taatcttcac
      481 acccagcgcc tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac
      541 gacgacattc ccggtctggt aatggatggc gtggtagacc ttgggattat cggcgaaaac
      601 gtgctggaag aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc
      661 ttgcgtcgtc tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc
      721 tgggacggcc cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc
      781 aagcgttatc tcgaccagaa aggcatctct tttaaatcct gcttactgaa cggttctgtt
      841 gaagtcgccc cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc
      901 acgctggaag caaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctt
      961 atccagcgcg atggtgaaat ggaagaatcc aaacagcagc tgatcgacaa actgctgacc
     1021 cgtattcagg gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc
     1081 gaacgtctgg atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg
     1141 ctggcgggtg accaacaacg cgtagcgatg cacatggtca gtagcgaaac cctgttctgg
     1201 gaaacgatgg aaaaactgaa agcgctgggt gccagttcaa ttctggtcct gccgattgag
     1261 aagatgatgg agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg
     1321 cggagcaaca acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc
     1381 gcactgttaa cgatattctc gataacgtga aagcacgcgg tgatgacgcc ctgcgggaat
     1441 acagcgcgaa gtttgataaa accacggtta acgcgctgaa ggtgtctgct gaggagattg
     1501 ccgccgctag cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaaa
     1561 atattgaaac cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag
     1621 gcgtgcgttg ccagcaggtc acgcgcccgg tcgcttcagt tgggttgtat attcctggcg
     1681 gctccgcccc gctcttctca acggtattaa tgctggcgac tccggcgcgt attgcgggct
     1741 gtaaaaaagt ggtgctgtgc tcaccgccgc cgattgccga tgagatcctt tatgcggcgc
     1801 agctgtgcgg tgtgaaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg
     1861 cgtttggtac ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg
     1921 tcaccgaagc gaaacgtcag gtgagccagc gtctggacgg tgcggctatc gatatgcccg
     1981 caggcccgtc ggaagtgttg gtcattgccg acagcggcgc aacgccggat ttcgtggctt
     2041 ctgatttgct ttctcaggct gaacacggcc cggactcaca ggtgatttta ctgacgcctg
     2101 acgctgatat ggcgcatcaa gttgccgaag ccgtcgaacg ccagttagca gaactgccgc
     2161 gtgccgaaac cgcccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag
     2221 cgcagtgcgt agagatctcc aaccagtacg gcccggagca cctgatcatc cagacccgca
     2281 acgcccgcga tctggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt
     2341 caccggaatc ggcaggtgat tacgcctccg gcaccaacca cgttctgccg acttacggtt
     2401 acaccgccac ctgttccagc ctcgggctgg cagatttcca gaagcgtatg accgtgcagg
     2461 aactgtcgaa agaaggtttc tccgcgctgg cttcgaccat tgaaacattg gccgccgccg
     2521 agcgcctgac cgcccataaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag
     2581 catgagcacc gtgactatta ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta
     2641 tcagtcggcg cgtcgtctgg gcggtaatgg cgacgtctgg ctgaacgcca acgaataccc
     2701 cactgccgtg gagtttcagc ttactcagca aacgctcaac cgctacccgg aatgtcagcc
     2761 gaaagcggtg attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt
     2821 cagtcgtggc gcggacgaag gtattgaact gctgattcgc gctttttgcg aaccgggtaa
     2881 agacgccatc ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ccgaaacgat
     2941 tggcgtcgag tgccgcacag tgccgacgca ggacaactgg caactggact tacagggcat
     3001 ttccgacaag ctggacggcg taaaagtggt ctatgtttgc agccccaaca acccgacagg
     3061 gcaactgatc aatccgcagg attttcgcac tctgctggag ctaacccgcg gaaaagccat
     3121 tgtggttgcc gatgaagcct atatcgagtt ttgcccgcag gcatcgctgg ctggctggct
     3181 gacggaatat ccgcacctgg ctattttgcg cacactgtcg aaagcttttg ctctggcggg
     3241 ccttcgttgc ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat
     3301 cgccccctac ccgctctcga cgccggttgc cgacattgcg gcccaggcgt taagcccgca
     3361 gggaatcgtc gctatgcgcg aacgagtgac gcaaattatt gcagaacgcg aatacctgat
     3421 tgccgcactg aaagagatcc cctgcgtaga gcaggttttc gactccgcaa ccaactacat
     3481 tctggcgcgc tttaaagcct ccagcgcagt gtttaaatct ttgtgggatc agggcattat
     3541 cttacgtgat cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac
     3601 ccgtgaagaa agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag
     3661 aagtatcttt ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag
     3721 gtggaccgtt ttgacaaact cgcctttgaa ccaggcgtga tcccggaact gctgaagctg
     3781 caaaaagcgg gctacaaact ggtaatgatc accaatcagg atggtctggg aacacaaagt
     3841 ttcccgcagg cggatttcga tggcccgcac aacctgatga tgcagatctt cacctcgcaa
     3901 ggcgtacagt ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc
     3961 cgtaagccga aagtaaaact ggtggaacgt tatctggctg agcaagcgat ggatcgcgct
     4021 aacagttatg tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt
     4081 aatggtttac gctacgaccg cgaaatcctg aactggccaa tgattggcga gcagctcact
     4141 aaacgagacc gttacgctca cgtagtgcgc aacaccaaag agacacagat tgacgttcag
     4201 gtgtggctgg atcgcgaagg tggcagcaag attaacaccg gtgttggctt ctttgatcac
     4261 atgctggatc agatcgccac ccacggtggt ttccgtatgg aaatcaacgt caaaggcgac
     4321 ctctatatcg acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta
     4381 aaaatggctc ttggcgacaa acgcggtatt tgccgctttg gttttgtgct accgatggac
     4441 gaatgccttg cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atacaaagcc
     4501 gagtttacct accagcgcgt gggcgatctc agcaccgaga tgatcgagca cttcttccgt
     4561 tcgctctcat acaccatggg cgtgacgcta cacctgaaaa ccaaaggtaa aaacgatcac
     4621 caccgtgtag agagcctgtt caaagccttt ggtcgcaccc tgcgccaagc catccgcgtg
     4681 gaaggcgaca ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata
     4741 ccggctgcgc caatctgaac tcggtgaagt cagccattgc gcgccacggt tatgaaccaa
     4801 aagtcagccg tgacccggac gtggtgttgc tggccgataa actattttta cccggcgttg
     4861 gcactgcgca agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag
     4921 cctgtaccca accggtactg ggcatctgct tagggatgca actgctgggt cggcgcagcg
     4981 aagagagcaa cggcgtcgac ttgctgggca tcattgacga agacgtgccg aaaatgaccg
     5041 actttggtct gccgctgccg catatgggct ggaatcgcgt ttacccgcag gcaggcaacc
     5101 gcctgtttca ggggattgaa gacggcgcgt acttttactt tgttcacagt tacgcaatgc
     5161 cggtcaatcc gtggaccatt gcccaatgta attacggcga accgttcacc gcggcggtac
     5221 aaaaagataa cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta
     5281 agttgctgaa aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg
     5341 caccgtggtg cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc
     5401 gctgccgcgc ttacaggatt acgccgcgca gggtgccgaa gtgctgcatc tggtggatct
     5461 gaccggggca aaagatccgg caaaacgtca aatcccgctg attaaaaccc tggtcgcggg
     5521 cgttaacgtt ccggtgcagg ttggcggcgg tgtgcgtagc gaagaagacg tggcggcgtt
     5581 actggaagcg ggcgttgcgc gcgtggtggt tggctccacc gcggtgaaat caccagaaag
     5641 ggtgaaaggc tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg
     5701 tattgacgag caaggcaaca agcaggtggc ggtcagcggc tggcaagaga actcgggcgt
     5761 ttcactggaa caactggtgg aaacctatct gcctgtcggc ctgaaacatg tgctgtgtac
     5821 cgatatctcg cgcgacggca cgctggcagg ctctaacgtc tctttatatg aagaagtgtg
     5881 cgccagatac ccgcaggtgg catttcagtc ctccggcggt attggcgaca ttaacgatgt
     5941 tgctgccctg cgaggcactg gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg
     6001 taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc atgtctcgac
     6061 gttcgtgatg gtcaggtggt gaaaggcgta cagtttcgca accatgaaat tattggcgat
     6121 attgtaccgc tggcaaaacg ctacgccgaa gaaggtgcag acgaactggt gttctacgat
     6181 atcaccgctt ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa
     6241 gtgatcgaca ttccgttctg cgtggcgggt gggattaagt ctctggaaga tgccgcgaaa
     6301 attctttcct ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaacc
     6361 ttaattactc gcctggcgga tcgctttggc gtgcaatgca ttgtggtcgg cattgatacc
     6421 tggtacgacg gcgaaaccgg taaatatcat gtgaatcaat ataccggcga tgaaagccgc
     6481 acccgcgtca ctcagtggga aacactcgac tgggtacagg aagtgcaaaa acgtggagcc
     6541 ggagaaatcg tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgatctcgaa
     6601 caactgaaaa aagtgcgtga agtttgccac gtcccgctaa ttgcctccgg tggcgcgggc
     6661 accatggaac acttcctcga agccttccgc gatgccgacg ttgacggcgc gctggctgct
     6721 tccgtattcc acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag
     6781 ggcgtggaga tcaggatatg ttaacagaac aacaacgccg cgaactggac tgggaaaaaa
     6841 ccgacggact aatgccggta attgtgcaac acgcggtatc cggcgaagtg ttaatgctgg
     6901 gctatatgaa cccggaagcc ttagacaaaa ccatcgaaag cggcaaagtc accttcttct
     6961 cgcgcactaa acagcgactg tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag
     7021 taaatattgc gccggactgc gacaacgaca cgctactggt actggcgaat cctatcggac
     7081 caacctgcca caaaggcacc agcagctgct tcggcgacac cgctcaccaa tggctgttcc
     7141 tgtatcaact ggaacaactg ctcgccgaac gcaaatctgc cgacccggaa acctcctata
     7201 ccgccaaact gtatgccagc ggcaccaaac gcattgcgca gaaagtgggc gaagaaggcg
     7261 tagaaaccgc actggcagcg acggtacatg accgctttga gctgaccaac gaggcatcgg
     7321 acttgatgta tcacctgctg gtgctgttgc aggatcagga tctggattta acgacggtaa
     7381 ttgagaacct gcgtaaacgg catcagtgag ttgcagggta agcggatgcg atgttgttgt
     7441 cgcatccggc aaaaaaaaag ggcaaggtgt caccaccctg cccgttttct ttaaagctgc
     7501 aaagattact tcgcgttata attacgcaga gcgttacgcc ccagcacaat ccccgcgcca
     7561 accatgccgc ccagcagcac cgccagaatc aaggtaattg cctttttcgg gctatcgcga
     7621 cgaataggta acgtcggttt catcacataa cggtaagcat gaatatcaag atcatcaacc
     7681 tttaagtttt caatatccaa caggttttga cgtgtctgat agtagtttgg tgagaacacc
     7741 aacggacggg tcgcctcatg cttaatcatc gactccagcg cttcgctccc caaaaggaac
     7801 aacgtatctt gtgtcacatc ttcagtctgc tgaacctgtg gctttgccac ctgcgcctga
     7861 ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg
     7921 actacttcct gcgttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtct
     7981 ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat gtattgggcc
     8041 aacttcattt gtgctccctc tgcagtttgc ccaacataag aaacagtcaa tggtaattgc
     8101 tggttcttaa cagaaggttc gatggtaagt ttttctggtt cttcctgatt atccagcgtt
     8161 tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg taaatccgat
     8221 actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc agcaatttgc
     8281 cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa
     8341 tatccaatag ccagggcaat agccacaatg acggaaatga tgattgtcat cttgccacgc
     8401 cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa
     8461 acattattat tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata
     8521 gtttagcgat aatcctagtg aatgctatag gataagtgat aaaaataatg aattattggg
     8581 gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc
     8641 aaagagatcg cgggtgtata ccttatctgc cacatcttta agctcttctg ccattcggtt
     8701 agagataatg acgtcggctt gttgtttgaa gatggcgaga tcacgttcca gccgagagtt
     8761 gaagaatgag tcttctttca tcactggctc gtagatgatc acttcaacgc ctttcgcctt
     8821 gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgaac cgctcttcat
     8881 aatcagacga taaataccca ccacttgcgg tttgcgtgac aaaatggcat cggcaataaa
     8941 atccttacgc gtgcggtttg catcgacaat tgccgagatc aggttattcg gcacagactg
     9001 atagttcgcc agtaactgct tggtatcttt cggcaagcaa taaccaccat aaccaaacga
     9061 cgggttgttg taatggttgc caatacgcgg atcgagacaa acgccttcga taatctggcg
     9121 agtattcaga cctaaacttt ctgcgtagct atccagttcg ttaaagtatg ccacgcgcat
     9181 cgctaggtag gtgtttgcaa aaagtttaat cgcttctgct tcggtcgaat cggtgaacag
     9241 ggtcgggata ttttgcttaa tcgcgccttc ctgtaacaac gcagcaaaac gttctgcgcg
     9301 ttctgaacgc tcaccgatga caatacgtga aggatggaga ttatcgtaaa gggctttacc
     9361 ttcacggaga aattccggag agaaaataat attttcagta cgatatttct tatgcatcgc
     9421 ttcggtaaaa ccaacgggaa ccgttgattt gatgaccata accgcataag gatttatctc
     9481 aactacgtct ttaattactg attctacact ggatgtattg aaataattag ttttaggatc
     9541 atagtcggtt ggagtggcga tgatgacata atcagcatcc cggtaggctt catttttatc
     9601 taatgtggca ttaaagtgta ttttatctga ttgcaaaaac tgctgaattt ccttatcaac
     9661 aataggagat atccgatcat tcagcatagc aacgcgtgac ggtaaaatat ctaatgccac
     9721 aacctcatga ttttgtgcga ttagaagccc gtttgacaag cctacatagc cagtaccgga
     9781 aatggtgatt ttcatttcat gctctcagaa ttaacttaac tgtgaatcat gatgttttta
     9841 gcatcctgat aagagctaaa tgttttaacg ctacgttatt agctggtcaa cgctacgaaa
     9901 aacaattcag attttcttta taagaatagc tgatatttat taagttaata ttaagcagtg
     9961 aaatttagtc tataagataa atggcaaaaa aaaagcccgg tgcaatatac gccgggcctc
    10021 aattttactg ttggttaaat cagattaatc cagccattca gtatggaaca caccttcttt
    10081 atcaatgcgc ttataagtat gtgcaccgaa atagtcacgc tgtgcctgga tcaggttcgc
    10141 aggcagaaca gcggcacggt agctatcgta ataggcaacc gcagcggcga aggtcggaac
    10201 cgggataccg ttctgtactg cataagcaac gacatcacgc agcgcctgct ggtagtcatc
    10261 ggcaatttgc ttgaagtacg gagccagcag caggttagcg atctgcggat tttcggcata
    10321 tgcatcggtg attttctgca ggaactgcgc acggatgatg cagccagcac ggaaaatctt
    10381 cgcgatttcg ccgtagttca gatcccagtt gtattcttca gacgcagcac gcagctgaga
    10441 gaagccctga gcgtaagaaa cgattttgcc cagatacagc gcacggcgaa ctttttcgat
    10501 gaactcagcc ttgtcgcctg ctgactgagc ttgcggacca gagagaactt tagatgcggc
    10561 aacgcgctga tctttcagag aagagatata acgtgcaaac acagactcgg taatcagcga
    10621 cagcggttct ccgagatcca gcgcgctctg gctggtccat ttaccggtac ctttgttagc
    10681 cgcttcatcc aggatcacat caaccaggta gttaccgtct tcatcttttt tagtgaagat
    10741 atctttggtg atgtcgatca ggtagctgct cagttcaccg ttattccact cggtaaaggt
    10801 ctgtgccagt tcttcgttag agagattcag gccgcctttc aggagagaat aggcttcagc
    10861 aatcagttgc atatcaccgt attcaatacc gttgtgaacc atcttcacat aatggcctgc
    10921 gccatcagca ccgatatagg taacgcacgg ttcgccatct tcagcaacgg cggcgatttt
    10981 ggtcaggatc ggcgcaacca gttcataggc ttctttctgg ccaccaggca tgatggacgg
    11041 ccctttcagc gccccctctt ctccgccgga aacaccggta ccgatgaagt taaagccttc
    11101 ggcagaaagc tcacggttac gacgaatggt gtcctggaag aaggtgttac caccatcgat
    11161 gatgatgtcg cctttatcga gatatggttt gagcgaatca atagcagcat ccgtgcctgc
    11221 acctgctttc accattaaca ggatgcgacg aggcgtttcc agagattcaa caaactcttt
    11281 caccgtatag taaggaacca gtttcttgcc tggattttcg gcaatcactt cttccgtctt
    11341 ttcacgggaa cggttgaaaa tagagacggt ataaccacgg ctttcgatgt tgagcgcaag
    11401 gttgcgcccc atcactgcca taccgactac gccgatctgt tgctttgaca ttgtttactc
    11461 ctgtcagggg gtggtcaccg ccgagtgtgc ggcttaaatg tggtatagat gttaactcac
    11521 tcatagagat ggtgaatagc attacctttg ctataccaca ctgaatatct caccttttcc
    11581 gtcttttaaa acttatttat atgaaattac tgaattactt gttcagtaac tcaagaataa
    11641 gttttgtctt ttcttccatc agcggcacat caccgcgaga ttccacattc aaccgcacca
    11701 ccggttcggt gttggaggtg cgcaggttaa agcgccagtc ggcaaaggtc atgctgatgc
    11761 catcggtgcg atccaccgcc agcgcctcgc ggctaaaatg ctgttccacg cggttaatcg
    11821 cctcaacggg ttgcgccagt ttgctgttga tctcaccgct tgccggaaac gctgccatcc
    11881 ggtcgcgcac cagttcgccc agcgtttttc ctttcaggca caccagttcg gcgaccagca
    11941 gccacgggat catgccgctg tcgcagtaag cgaaatcacg gaagtaatgg tgggcgctca
    12001 tttcaccacc gtagatggcg tcttccttgc gcatccgttc tttaataaag gcgtgtccgg
    12061 ttttcgacat caccggcgtg ccgcctgcgg cagtcaccac atcaacggtg ttccaggaga
    12121 gacgcggatc gtggatgatc ttcgcgccgg gatttttttc gaggaatgct tctgccagca
    12181 ggccgacaat gtagtagccc tcaataaact gccctttttc gtcaaacagg aagcagcggt
    12241 caaaatcgcc atcaaaagca atgcccatat ccgcaccgtg tttgatgacc gcattgcggg
    12301 tgtcgtcgcg gcattccggc agtagcgggt taggaatacc gttggggaaa ttgccgtccg
    12361 gcgtgttgtg cactttgatt aattccacgg gtgcgccaag ggctttaaag cgggcttcga
    12421 tggcgtccac caccggaccc gctgcgccat tcccggagtt aatcaccagc ttgagcggcg
    12481 tgaggttttt gacattgata taaccgaaca ggtgatcaac gtaagcgtca cgcagattga
    12541 tttgctgata gcgaccgcgt ttggtttcat caacgggagg aaagtcgttg gcttctgcca
    12601 gacgctggac atcgcgcagt ccggtatcac cgctgatcgg gcgagcccct tcgcgcacca
    12661 gtttcatgcc gttgtaatcc atcgggttat ggctggcggt aacttcaatg ccgccatcca
    12721 cgccgagatg gaacgtggcg aaatagatct cttcggtgcc ggacatgcca atatccagca
    12781 catcgacgcc cgcatcctgt aaacctctcg ccagcgccag ttttaaggtt tcgctggtga
    12841 ggcggacatc accgcctaac acaatggttt tcggtttgag aaattcgcca taggcgcgac
    12901 caatgcgcca ggcgatatct tcattcagtt cttcgcctaa tttcccgcga atatcatagg
    12961 ctttaaagca ggttaatttt ttcatgtcgt tacccttttt caggcaatag ttggccctga
    13021 ccgaaacggc caattttgtt tttgtcgtta tttacatctc ggtttcgcat tcattgcctg
    13081 atgcgacgtt tacacccgtc cgtagcgatc cgcgaaacgc accacatcat cctcttcgag
    13141 ataagagccg gagcgcactt caattaaatc gagcggaatt ttccccgggt tttccaggca
    13201 atgcatcgcc cccagcggaa tataaatgga ctcgttttca ccaagcagtt tgatatcacc
    13261 atcaatggtg acttttgccg ttcccgcgac aaccacccag tgttccgcgc ggtgatggtg
    13321 catctgtacc gacaagccct cgcccggttt cacggtgatg cgtttcacct ggtagcggtc
    13381 gcccgcgtcg atagagtcat atttgcccca cgggcgatag acttcgcgat gcacccgatg
    13441 ctcatggcga ccatcggctt tgatctgctc gaccactttt ttcacatcct gcaccgcgtt
    13501 acggtcggca atcagcaccg catctttggt ctgcaccact accagatctt tcaccccgac
    13561 ggtggttacg aggccagatt cggcgtacac atagctgttt tcagttttgt gattaatcac
    13621 atcgccgtgg caaacgttgc cctcggcggt gtgggcgcta atttcccata atgaggacca
    13681 ggagccgaca tcgctccagc ccgcatccat cggcaccaca acggcatctg ccgtacgttc
    13741 catgaccgcg taatccaccg actcttccgg gcaggcgaga aacgcttctt catccacacg
    13801 aataaaatcg agatccggat cgacggcgct catcgctttt tcacaggcat cgagaatatc
    13861 cggacgatat tttttcagtt cttcgagata gcgtccggcg cggaacagga acatgccgct
    13921 gttccagtaa tattcgccgc ttgccacata ggcctgggcg gtttccagat tcggtttttc
    13981 gacaaactgc gccacttcaa aggccaccgt atcctgctca cccgcagaca cttcaccgcg
    14041 acgaatatag ccataaccgg tttctggtag atccggcaca atgccgaagg tcaccagctt
    14101 gcccgcttcg gcatatggca tggcattacg cacggcggca cggaacgcgt cttcatcggc
    14161 aatcacatga tccgccgcca ataccagcat taacgggtcg ctctccgggc tatgacgttt
    14221 tgccgccagc gccgccagcg caatggcagg tgccgtgttt cgccctgccg gttcgagaat
    14281 aatgttctcg gtaagtttgt tcagttgacg cagctgttcc gcgacaataa agcggtgctg
    14341 ctcattgcaa atcaccaccg ggctttcgca ctccacgccg ttcaggcggc agatggtggt
    14401 ttgcagcatg gtgagatcgc ctttcaggca taaaaactgc ttggggtaaa gtacgcggga
    14461 aagcggccat aagcggctac cggagccacc tgccatcaca actggataga gtttcgactg
    14521 cgccataatt atccccgaat atcatttata aattgacgta acacgttctc tttatcgagt
    14581 gtgcgttcgg catattcacg tgccaccgtg ttgtgtttgg gcagcaggag cgcctgacgg
    14641 atccccgcca ccagcgcctc gaccgattcc ggttctacgc aaaccgcaat gcccggaaag
    14701 gtttcgcaaa gctgccccag ttctgtgtgg gcctctgcgg taatcaccgc gttaccgcct
    14761 accgccagaa tattggtcag tttcgacggc aatacggcat ctgccgcgcc gcgtttttgc
    14821 accaccagat ggcaatcgcc catcttcagc agtgcgggta aagcgtcata cgattgcagc
    14881 gggaaaaatt gcatgttgcg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg
    14941 ccgccgccct gcccgacaat ggcaaaaatc agcggttcat cgcgcagacg atcggcagct
    15001 tcaataacgt tttccagccc ctgcttttca ccaatattgc cggagtaaag aatgattttt
    15061 ttgttatccg gcaggtcaag ctggttacga agggcgtcaa catcggcatc tgcaacatgc
    15121 tgaaaacggg caatttccga ccagttgggg aagaagatga cgttttccgc cgccacgcct
    15181 ttttcgatgg ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc
    15241 agtccgctac gttcgaacgc cgttgccagc tgtgccactt tgctgccttt gccttttccg
    15301 gcaaggccca gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc
    15361 gcaccagaaa gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggcaccacg
    15421 ccgataatgc gatccggctt ccagcgacgt tgcgccatca gaggaaagaa actgctgacg
    15481 gcaaaactgc cgagatgtaa caggcgtttc agggtgctcg gctgttttgg cacgtacagc
    15541 gggcagcgcc acaccgtggc ggccccctct tctcgtttgt accgccaggc ggaatagttc
    15601 tcgcccacct gccactgcgg gtagtaaggc ggtgcggtaa taacccgcac ctcatgacct
    15661 tgtgccgcca gccattccac catctcgccg gtgtatttgc cgataccggt taactccggc
    15721 gagtagttaa tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag
    15781 cgagaaaata ggcgcggctg ttagcgtgaa catcgttact ggcgagcagc gcgtccggcg
    15841 tcagccagcg gtaatcgtca tgctgctcat ccggcagtat cagctcttct tcagcgactc
    15901 tgaagcgaaa accgagcacc acatagtgag tggtgaaatc cgtgccggag aagttatcgt
    15961 cataaaagtg ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca
    16021 gttccgccat cgtcagccgc tcgaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc
    16081 ctcccggcac aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca agcagaaact
    16141 caccgcgact gttctcgaca ataaagtcga gagagacaag cggggtggag cgcactaccg
    16201 tggcaaagtc ttcctgacgt aaaaacatca ttacccccga aagcggtctt gattctcaag
    16261 gaaccactgg taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag
    16321 ctgatgcagg cgcgtcacat ccagcagttt gcgcggcgta ccatccggtt tgctggcatc
    16381 aaaaaccacc cgacctttgt aacccaccac tttggcgatg gtttgcgcca gctcgcggat
    16441 agtgcagtca acgcccgtgc cgacgttaat gtgcgacagc atcggctggg tgttctccag
    16501 ccagacttcg tgcgccagct ccatgacatg aatgctcgcc gccgccatat catcgacgtg
    16561 caggaattca cgcatcggtg taccgctgcc ccataccacc acgtccggtg cattctgtgc
    16621 cgtcgcctcg tggaagcgac gcagcaatgc agggatcaca tgcgaattac tcgggtggaa
    16681 gttgtcatgc gggccataca ggttggtcgg catgactgag cggtaatcgc gtccgtactg
    16741 gcggttgtat gattcgcaca gtttgatccc ggcgattttg gcaatggcgt aaggctcgtt
    16801 ggtcggctcc agcgtgccct gcaataactc gctttctgcc atcggctgtt ttgccagttt
    16861 cgggtagata caggacgatc caagaaacag cagtttgttc acgtcgttct gatgcgcggc
    16921 gtgaatgatg ttgctctcaa tcatcatgtt ctggtagatg aaatccgccg gataggtgtt
    16981 gttggcaaca atgccgccca ctttcgccgc cgccagatag acctggtcaa tgcgttcgct
    17041 ggcaaagaaa tcatgcaccg cgcggctgtc cagcaggttc agctcgtcgc gggtgcgtaa
    17101 taccagttcc acatcaccgc gctgttcgag ctgccgcctg atggcagaac cgaccatccc
    17161 acgatggcca gcgataaaaa tgcgttgttt actcatgctt atgactccag cgcgatcgcc
    17221 acgtcgtagc cgtgagattt cagcagagag tgttttttcg ccgcttcgag gtcattagcc
    17281 accatttcag acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc
    17341 gctttggtcg ggtcgccgag cagcgtttca acttcagccg gacggaagta acgcggatca
    17401 acggcgataa tcacatcacc cggtttaacg cccggcgcgt catgcccggt gacggaaacc
    17461 acaatgccct tctcttcaac acccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc
    17521 gctgccattt ccacgaactg acgcacggag tactgaacgc cggtcgcgat aacgaaatct
    17581 tccggctgtt cctgttgcag catcatccac tgcattttta cgtagtcttt ggcgtggccc
    17641 cagtcacgca gggaatccat attgccgagg tacaggcacg actccagccc ctgggcgatg
    17701 ttggcgattg cgcgggtgat tttgcgggta acgaaggttt cgccgcggcg cggggattca
    17761 tggttgaaga gaattccgtt acaagcgtac atgccgtagg attcgcggta gttaacggtg
    17821 atccagtagg cgtacagttt ggcgaccgca tacggagatc gcgggtagaa cggcgtggtt
    17881 tctttctgcg gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa
    17941 cgagttttct tttccagacc gaggaagcgg atcgcctcca gcaggcgcag cgtacccatc
    18001 gcgtcgacgt cagcggtata ttctggtgac tcaaaagaga ccgcaacgtg gctcattgcg
    18061 cccaggttgt acacttcatc cggctgtact tcacgcaaaa tgcgcgtcag gttagaggta
    18121 tcactcaggt cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa
    18181 atgtgatcca cgcgctcggt gttgaatgac gatgcgcgac gcttaatacc atgcacctcg
    18241 taaccttttt ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg
    18301 atgagagcga cttttgacat gtcttattcc tctgtatttt tgaatttatt cagtttcaac
    18361 gcgttcgcgt atcaccactg cgggattccc ccggcaaacc acatttgccg gaagcgattt
    18421 aaaaacactg cttcgtgcac ccacgacggt gccgtcgccg attgtgacgc ctggggcaac
    18481 aaagacatcg gttgccagcc agcatttctc gccaatcaca ataggcgtgg cgttaatggt
    18541 gaaatgttga cttgcatggt cgtggctacc ggtgcataaa taactttttt gcgatatcac
    18601 cgaatgtgcg ccaatggtta tttcaccgag ggtatataaa ttgacgtcat cgccgaccca
    18661 cgcgtaatca cctaaggtta atttccacgg ataggtaatt tttactgacg gacgaataac
    18721 tacgtttttt cctatttttg ctccgaataa acgtaataaa aaagcccgcc agcgatacaa
    18781 tacttgtggc gaccaggcaa atattgttgc ctgtactgcc caccataatt gcactttaat
    18841 agcgttgccg ccccggaacc ctttcggcac cgagaaaccg cttaaatctt gcattggttt
    18901 tccttatatt cagactttgt tatataaggc tttcgtcttt gaggtagtac gttggcgtaa
    18961 atgccaggat aattcagccc aaaagccagg cacatgtaat atttgtcgtt ggactttttt
    19021 cgcgtcagca cacaattcca tattattggt ggtagatacc ccacccatgg aaaattcaga
    19081 caccaggcca ttgagttttt taaatgcata accggcttta tacattttgg ctgccagcgc
    19141 gtagtcggaa gaaactttat attccaggtc ataacgccat tttttcaagc cggatacagg
    19201 gaaaaatatc gcctgatgac tggcgggcag gctgtgataa atataccagc ccggtttggc
    19261 gctacgttta attttatgcc cgtcgccaaa atccagcagc gcatcgccgg tgatcatcat
    19321 gttatctttt tgcattttta acttacggac aaaatttgcg gcatcctgat gaaaaatatc
    19381 gcccgaattg agaaacaacg cgaacttgcc ttgtgccatc gtaatgcctt tgttcatggc
    19441 gtcgtagata ccgttatctg gctcgctgac aaagcgtagg ttatagatac cattgagatt
    19501 ttccagatac tcacgggtgc cgtcattgga accgccatcg acaacaatcc attcgaagct
    19561 gatatcttcc gcctgcgcca gatgcgccag cgaggcatgt gttttgacta tcccttcgag
    19621 gttacgaaac gcgacagtga ttatgctaag caacatgttc ttattcttac ctcgtaatat
    19681 ttaacgcttt tcgcaaaata aatggacaga cgattaaaaa tgcatattcc gggctaaata
    19741 tcgaaccggt aaaaaacagc gataccggag taaaaagata taattgcacg cgaaaattgc
    19801 ggttatcacc aaaagcgttg atcatcattt ttatcacttt ccccatgtac cacagcgata
    19861 aaaacactgc gaaccaggaa aaataaataa tcagcagata caaaccattg tctatggttt
    19921 ttccgacatc cgcaccgtta aatattccga atgatgcgac gtattcataa agtgagccaa
    19981 atctgactac accgtcaata tgggtcaaag aataaccgac catcaccagc ggaccgacaa
    20041 tacgataata cgatgacgat ccttccgtac ctaaatcgcc cagacgggtg gaaatataag
    20101 gaaacgcgat taccacacca accaggaata cagccagaga aatcaatgcc aacggtaact
    20161 ttttcttaat cgcctcttta ttcagatatt gaaatgccca ctccagcaaa taaaacagga
    20221 taaaagtcat aacccctgaa aacgatccgg ataatattat ccctgcgaga atcatagcat
    20281 cggttttagg cgttttgata ccaaactgtt tgatgctgag ccaaattgag attaatgcca
    20341 gagcgaaaaa tgccggttcg aaataaagtg ctgtcgtgcg cttgccgccg aatttaatga
    20401 aattcagtac atagctgttg ctgtaaatca gatatttcga aatcgactcc ataatactgc
    20461 tgccaccggt gaggataatt tgcgccatct ccaccgccgc cagcgccacc accagcccga
    20521 tcaccagata aaagaaacgt aatatcttgc gatggttgtg tggcgaaatc gttttaaagc
    20581 gaatactcca caccatgcca ataatgatca caatatagac aaacagcatg gttgaagtga
    20641 cgtatttact ggcatccagc gactgaccaa acagatagtt aaacgccgtg agtcccgcac
    20701 caatccctaa ggcaatcatc aattttttaa cgctgatgcg ttctaaaaac agcagtaaca
    20761 agacaggtaa aaaagtgacg atggtgatgg ggaaactttc gccaagctgg gcgattttga
    20821 cgttaaccag caggtagatc agcggcagca gcaggtagct acagattctg atagaagttg
    20881 acatactcct ccagcatctg ttgtccactg taggtggcgc ggctgcgttg gctgaactca
    20941 gccagcgtgg taccaaatat cgcctgcgcg atttccggtt tgcttaactg caccagttgc
    21001 agcacgtctt cttcgtcgac ggttttaccg ccggattttt gcaacacttc ccgtgccgcg
    21061 tcgctatggg tggcaatcac cggcacgcca attgataacg cctcacacaa aatcagcggg
    21121 tagttatcga cgcgagaact gaataccagc gcatccatct gattgagcgc gctcatcaac
    21181 ttgcgcttgt cagtttcaaa gccgtgatta accacgttgc cagcggtgaa cggcgagaac
    21241 ttaccaaagg tatgcagttc gattttgtcg cccagcgcca tcatctcacg caccagttgc
    21301 tggttagtct tgccgtcgta acgcaagtca tgcgccacca ccgcgatttt cggcttgccc
    21361 tgggtttcgc gcaccggagg caagtccgcc agaatcgctt cggttgccat atcaatacca
    21421 ttattgataa tccggcaacg ccctggaccg tacaggctat tgaaagcgtc agccacatgc
    21481 tggctggggg aaataaactg acagcccagc gccagcatct cacggaataa ctggcgtttg
    21541 cccgccacca gctggtgtgc gcgatcaatc ttcaccggcg gatagttatt taaggtcggg
    21601 catttctggc agcccgtttt ccagccttcg caaccgtcgg taaaggcgca gcgtccggta
    21661 acactccagt ggtcgtgcag cgtccagacc agagtgacgt ccggtttatg gtttttcact
    21721 ttttcgcaaa agcgcaccac gctcttaaga tttagccagt agctgtgcag cacatgaaaa
    21781 tgcaggacca ccggacccgg tgtgcgagta atggtgcgat ataactcatt gaaattgcca
    21841 aacagatcgc gattaaacag acgaaacagg gcaatattcg ccatcgcggt catccgcggc
    21901 gtatgtttga tgacctgcgg atagttctga tggctgacgc tctctttgcc gcctttgccg
    21961 taaccgtaga caaaatgtga cgccagcccc tgttgcagcg cacgctggtg gagatctaac
    22021 gccacacctg ctgccccgcc ttccgccagt cgcacattaa attgcagaat attcatttaa
    22081 ttaccttcac tcgcgctttt tctcccacca ccagcgcgtt gtccgggaca gagtcgagca
    22141 ccacgctgcc cgcacccacg gtgacgttgt taccaagcgt gatatcacca agaataatga
    22201 cgttggcacc gagttcgacg ccgttgccaa tgtgtggaca tgccatgtta tcggcaccac
    22261 gattgccgat agtgacgccg tggcgaatgg tgaaatcatc ccccgctacc acgtttttat
    22321 tgatcactac ggcgtaaccg tgatggatag taaagcggcg gccaatggtc gcggcggcct
    22381 ggatttcata accgaaaaag cattcggtga taatgcgata cagcaccagc agcggggccg
    22441 cccacagatt gttgaggacg ttctttttgc gccacaccga acaaaaatga gcaacacgat
    22501 aggcaagaac catgcagcac gggcgtaaac tccagctgtt ggcacgcaga tcttccagca
    22561 tctttagcgc ccccgaatac catcagccag acgtttgccg ttacgcaccg acagcagcgt
    22621 cagcaaagtg cgccaggtca tacgtttatt gcggatctgg tagagggtaa acagctgata
    22681 ttttttgctt gcacggtcaa atttatcttt gtgctttcgg taaaagtgga agtacccgga
    22741 gaattttttc ggtgacgagg tgatctgcat ttcaccgtga ttgatatgca ggatctgcgt
    22801 cgcttcttcc actttccacg gttcgccgta ctccaccacc atccgcagga agatgtcgta
    22861 atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc atgcccaagt
    22921 aaagacctga ttgccaatga tattgcgttt atagaacagg cggcgtgagt acggcgattt
    22981 gggatacagt ggtaagcttg ccggttggga ataaacttct ccctggcata cgtagtcgtt
    23041 agcgtacaaa aaggcgtgag tgaccagttg ctgtttatgg gcgaggaaga cgctcagacg
    23101 gttgggtgtc cattcgtcat cgtcatcgat cccggtaatg tattcccctt gcgccagcat
    23161 aatcgcctgg ttacgtaccg cgcacgcccc gctgttaatg tcgttgtgaa tgtaggtgat
    23221 acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg tggagcaatc
    23281 atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt ttatcgcccg
    23341 aatcgccagt tgttggcggt tccaggtcgg catatagatt gaaatcagcg gattctcttt
    23401 cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg tgagcgcctt
    23461 atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga cttatattcg
    23521 tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga gttcagaatc
    23581 acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc cacttctttc
    23641 aatgtgttga ccgcatagcg cgccaccatt aacgtggttc cagcgtgacg cccgacaatt
    23701 gctgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc gtagttttta
    23761 ctcgcccagc tcaccaattc ggcaaagcgt tcgctcatca acagttcaga aggatttggc
    23821 ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt agctgcggta
    23881 gtaatatcgc cctggccaat cagaatttcc gacaggccat tgacgttatt ggtacccaac
    23941 agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacccg tttattggtc
    24001 tgactgatca ccgccgccag gttggcgcag acaaaggttt taccgattga cgggctcacc
    24061 cctgtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag actggtacgc
    24121 agactgcgga tggcttcaat cgccagatcg gttggattcc ccaccgccag tagctggctc
    24181 tgtttatagc gtttaatccc tttgatggtt ttgacgctat cgcgcgcttt ctgccattcc
    24241 gacagcggga tgctggcata gacgctgata ccatgttctt ccagcacctg cgggctttcg
    24301 atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat caggccgagg
    24361 ataattgcgc cgaggataat cagtcctttc ttcggtttca gcacgccagg ctgggtgatt
    24421 gccgggtcaa caatgcgcac atcgccgacg gtgctggctt cggtgatttt cagctcctgc
    24481 tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg ggtcaggcgc
    24541 acaatctcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt ggctttctct
    24601 tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac tttggtgtac
    24661 agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc gatgttcacc
    24721 atcgaatcga gcaccgcttt cgcttccagc ggcagatcaa cagaatcttt atcctgacgg
    24781 aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg taactgttgc
    24841 gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt ttgttcctga
    24901 tagttacggg cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc accggtataa
    24961 gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct gttttgcagt
    25021 tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc cgggcgggcg
    25081 tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc cgcttgccca
    25141 cgggcgctaa agccgccatc actgctcagg gtgtagtttt tattgtccag cacattaagc
    25201 gtaaacacct ggcccgccat ctctttcggg cggttaaagg tagtcacttt caccgtctcg
    25261 ttctggcgtc ccatcagtcg ctcccagccc gccccgaaaa tcgggaacgt gtttttgctc
    25321 actgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg cgaacgaatc
    25381 aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgaacc aatgtcctgc
    25441 actaacgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact ataaatcggc
    25501 gttgcgaaga aggtgtaaac cacggcacag agggcaaata cagcggtgat gccaatcacc
    25561 caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc atcactgccc
    25621 gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc tctgcgttca
    25681 atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct gcaaacgttt
    25741 cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga ccaaacagca
    25801 tcactttgcc gcgcatctca ggtgccatct cgcataagcg ttcgatatgg cgcttttcca
    25861 tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt gcacagtggc
    25921 cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca gcgcccttac
    25981 cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa cgttgcagta
    26041 agcgttccgc cgtcggggaa cggcaaatat tgccgacaca gacaactaat atgttgttaa
    26101 acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac ggacaccgct
    26161 aatagttggc aacaactgat tgatcagacg gttccagcgg gaaaccgggg cggtggtgac
    26221 atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg acgtggcatc
    26281 ggacatatcc agctggtaga tattggcaat cttgccatta cgcccgccct cgcctttcag
    26341 cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac cttcagcatt
    26401 acccagggct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc tctgtttctt
    26461 cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga gaatatcgcc
    26521 gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct gcaaagaaat
    26581 gcgttcttca cgaccattgt gtgttagcac tacgttgcgc cagtcagcgg tgtcggtcag
    26641 gccacctgcg gcgttgatgg cgtcgagaat agtcagtggc acgttggtga tcgcctgttg
    26701 accggattta ttcacctgac cggagatata ggccttttgt gagcggaagg cggcgatatt
    26761 aacgtccacc tgcgggtcag cgatgtacgt cgctaagcgc ccggtaatat cactgcggat
    26821 ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa acatagtgcc
    26881 gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccgg ctggcgtggt
    26941 gagttccggg tgatcccaga cggtgacatt cagaacgtcg cccggcccga cgcgatactg
    27001 gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg ggcgtgggcg
    27061 taattggtca atcaggcgcg gggtcagcgg ataaacattc accattttgt cgagatcgaa
    27121 atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc tgcccggaag
    27181 tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt tcattttgga
    27241 tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact attaatagca
    27301 atatcgccat gcacatttac cttgcagtta attgaataaa aatttaactg gcatcagtcc
    27361 taaaaaaatt gatttcatcc gcaggctatt gacagaataa ttcagactgg tctttcaggc
    27421 atccagacac gctaccgccc ctggcttttt agccaccaat acactgattt agcttaattt
    27481 ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa attaacttcc
    27541 gaatataagg tgacattatg gtaattgaat attggctctc caataatgca ggaggaagtg
    27601 ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt tttaagaatt
    27661 atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa tatttaatat
    27721 tgcgcctcca catgcgatat ttcttaaata atgttttatt attaccactt ttaattcagg
    27781 gataatgtga ggttattacc ctcaaataat atgagataaa tagtgctgca acattgcatt
    27841 tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa taggttttgt
    27901 acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca cgctgattgt
    27961 gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg ccgaaaaact
    28021 accgccgagg cagcgcgacc gcgcacgggt taccgggctg ctgctggcga tgttaatgcg
    28081 cctgttactg ctggcgtcaa tctcctggct ggtcaccctg actcaaccgc tgttcagctt
    28141 ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt tcttcctgtt
    28201 gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca ataatcccac
    28261 acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac tggacgccat
    28321 tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac tggtcatgat
    28381 ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt taacgcaatt
    28441 tgttaacagt cacccgacga tcgttattct ctgcttaagc ttcctgttga tgattggctt
    28501 tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt acgctgccat
    28561 tggtttctcg gtgatgatcg aggcgctcaa tcagttggct atctttaacc ggcgacgttt
    28621 tctttccgct aaccagacgc tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag
    28681 cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg tggatcatgg
    28741 taaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac ttaatcttaa
    28801 ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata tcgatctcaa
    28861 cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc ggctggtggt
    28921 taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg acctgctaca
    28981 acagtctctg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc cgctggtgtt
    29041 cccggaaacc ttgccgttgt tacctgccct ggagcagttc cgtaatgccc gcacgcactt
    29101 tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa gtgacgtcac
    29161 tgaaaccatt gtcggtaact taccgaacga agtggaagag atcgacgccc gccatgatat
    29221 tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg aagatctggt
    29281 gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg ccgggctgtt
    29341 gatggaatat ttacagcgta ttccaaaacc tggcgaagaa gttcaggtgg gggattattt
    29401 gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta ttccgctgca
    29461 taaggatggc gagttggagt acgaggtgtg atggtatcgg tcattttacc caatagccga
    29521 aaggaataag atgagggaac cgcgccagcg gcgatttacc gccgggagcc cgggttgcca
    29581 gggtggtggc gattgagcca ccctggcacg ttcaccggct tgagcgttcc atagtagcga
    29641 ggaacatcaa gtgaacggaa cagccttcga tcttacatat tcagaatata atgatgccgc
    29701 cacgaacgcc tgatgcgacg ctttacgcgt cttatcaggc ctacaggact ggtcacctgg
    29761 taacctacat cttctccagc aacttcttca catccttgcc attgcgggaa tctttattcc
    29821 gctcggccca gtcattcagg cgacgtttcg cttcgtcctg tagatgtttg cgcagtaact
    29881 gatccacttg caggctgtaa ttgagttgct gccagttgcc ataaacccgc agcggtactg
    29941 gcgtttcttt caggaaatca atcagtttac tttccccgtt ccagccaccc acgacccgaa
    30001 tatcaaactg ggtatcgcag gtttgatctg ccagattcaa catgccttcc cctgtcagcg
    30061 ccagcacagg cgattgacct tgcatgtcgt ttaacgtcac gacgccatcc ttcaacgtca
    30121 aatcggtggt aaagcggtca agccgcgtca cgttatcaaa gttttcagcg gccttcacat
    30181 caccgccatt acgctctacc gcttgctgaa tcatctgctg gaagttcatc ccttccatgc
    30241 gcgtgtcggt catttcgaca tgtgcctgtc cttgccagtt gtggcggaat gcgtcggcat
    30301 ctatgtcagc accggagaaa tcaccagcca gtgacatttt tccggtcaac gaaatcggat
    30361 agttaaacgc cttcagaatg gtaccaatct caacgttttc cagccgtggc tggaagttta
    30421 tccgcggatt tattgatgtc gcgtccagcg tgcccggcag tgaaacctgt ccaccgttaa
    30481 gtttgccctg cagttgagta atttccagca aacccgactt gttggtcatt tgcgtggcaa
    30541 catctgtaaa attcattccg cgccagcgca cgttactggc ctgcaacaaa atatcagccg
    30601 taaagccttg cagtccctga taggccggtt catcaatacg cgaagaaatg accgggcgcg
    30661 gcaacgttga ttggctctgc ccctgctgcg cggcaccgtt ttcaccattc gcggtttcat
    30721 taagcgggat gaggttgtca agattcagtt gcgggaattg cagtctcagc tgccattccg
    30781 gtttctctgt cagcgtgacc tgtgcttgcc cgctcagcgt actgtcattg gcggtcaagc
    30841 tgatttggtt aaatgaaagg cgtttatgtg actcctgcca ctgggcctgg aaactcccct
    30901 gcccctgaat accttgtttt ggtaaatcgg caccctgcaa ctgccagtta atttgttcaa
    30961 tagccgccgt taaatcatgc ggataatcag aagcatccac cgtaccgtta agggatattg
    31021 tgagatcgcg ctgatcgcga ttaacccgcc cggagaactc aaatgagcca cgatgttggg
    31081 gatcttgttc catttgcagg cggatattgc ggattgtcac ctgctcgtca tcttcatgct
    31141 ggaacaccag cacgctgtcc gccaccttaa gactggatat atcaaacgac catccgcgat
    31201 cgtctgacag atccggcaag gtattgtcgc gcggtgcaac cggagcgtct tcactgcgca
    31261 ccgcttccgt ctgcggcgtc agttggatca ctgccccttt tagcatcacc tgcttaacgc
    31321 tcagttgatg actcagtagt ggtaaaagcg ccacgtccag acgcatgttg tcggcgcgaa
    31381 ccagtggctg gcttgcgccc tgggcggtga gagacattcg cccggagagg atactaagct
    31441 gcggccagac gtgccaacgc agtggcccgt cgagctgcaa ttgataaccg ctacgtgcag
    31501 caacttgctt gaccatatag tcgcggaaat cattcggatt caccagcaac actaacgcag
    31561 ataacccggc caccagcacg accaggagta tcatcagcgt cgtcagaaat cgtctcatgg
    31621 tatcctcaat gggcctgaat tagtctttat cgattcggct ggctaccgcg ccctgctggt
    31681 tgcgatattt cgcatcttca cggcggttgt aaggtcgcgc cgccgggccg gaaagcggct
    31741 caaagctcag cgcaccaatt aacatgcccg gacgcagcgc cagcggcagc ttaccggagt
    31801 tgtagaactc cagcacaatg caaccagacc agcccggatc gatacggtgc gcggtgacgt
    31861 gcaccatcag ccccagacgc gccaaagagg aacgcccgtc cagccagccc accagatcgg
    31921 ctggcagcgt caccgactcc agcgtcaccg ccagcgccag ctctcctggg tgaagataga
    31981 acgcctcgcc ctcgtcgaga acgatctcat cgctcatcac gcggtcaagc gcggcgctca
    32041 cttcatcttt gggaccgctc agatcgataa acgctgccgt gtgaccacgg aaggtacgaa
    32101 atttattgcc caggcgtaca tccaccgtcg cgccgttaat acgctccact ggcggacgtg
    32161 ggttgatcga caaacggcct tcatcaagcc aggcttcaat atctcggtca cacagacgca
    32221 tggcattttc tcctttcgcg catcactccc ttaacgccaa ttacgtcaag ggcataccag
    32281 gttatcactg aacggtacac aatttatcaa gcttattcaa agaactgact tattttcgct
    32341 ttcaatatat cgatcgcgat gcggtttttc ccgccgcgcg gcacgataat gtccgcatat
    32401 tgtttagaag gctcaatgaa ttgcaggaac atcgggcgca cggttttttg atattgcgcc
    32461 atcacggaat ccattgaacg cccacgctcg ttaacgtcac gcttgatgcg gcgcatcagg
    32521 cagatatcca gcggggtatc aacgaaaatg gagaagttaa gttcgtcacg caaacgcgca
    32581 tccgtcagca acaaaatgcc ttcgagaatg atgaccttct tcggctcaac cgtcaccgtt
    32641 tctttcatac gcgtatgttc aacatagctg taaaccggca ggtcaattgc cgagccgcgt
    32701 ttcaacgctt gtaaatgctc aagcagcagg ctgtgatcca tcgcgctggg atggtcgtag
    32761 ttggtcttaa cgcgttcttc catcgacaga tggctttgat ctttgtaata gcagtcttcg
    32821 ggaattacgc cgatgtgttc atcaccgact tgctcacgta attcacgata aagggtactg
    32881 gcaataagac tcttgccgga agccgatgcg ccagcgatac cgataatgac gcactgatga
    32941 gactgatcag tcatatattt agcgacctga ttaacctgga tgttaggaag ggggcgacga
    33001 agcgccaaac gcggcaatta tagggatttc atccgtctga taccagtcga atagcgttgc
    33061 cgcgcgctca gagttaattg ttgacaaaga attcccggtg gcaaattacg ttgatcagtt
    33121 ttatgtaagg taaaaaatgt tatacgcagt tgcgcaaatt atccgccttt acgtcacttt
    33181 atgagcaatt cgcatataaa atgtaaaact tttgtactag cataaacaca gaaacgaata
    33241 ctggcgacct ggtcttgcgg ataaagcggt aatgagcaaa caatcacagc atgtattaat
    33301 tgccctgccc cacccgctgc ttcacctggt cagtttaggt ttagtctcgt ttatctttac
    33361 ccttttctcg cttgagcttt cgcagtttgg cacccaactc gccccactgt ggttcccgac
    33421 gtccatcatg atggtggcgt tttatcgcca tgccgggcgc atgtggccgg gaattgcgct
    33481 gagctgttcg ctgggaaata tcgccgcatc catcctgctt ttttccacca gctcgctgaa
    33541 catgacctgg acgaccatca atattgttga agccgtggtc ggggcagtgc tgctacgtaa
    33601 attgctgccg tggtataacc ccttgcaaaa tctggctgac tggctgcgtc tggcactcgg
    33661 cagcgccatt gttccgcctc tgttgggggg tgttctggtt gtcctgctga cgcccggaga
    33721 cgatcctctc agggcatttt tgatatgggt actgtcagaa tccatcggcg ctctggcact
    33781 ggtgccgctg ggattgttat ttaaaccaca ctatctgctg cgccatcgca acccacggtt
    33841 gctttttgag tcgctgctca cgttagccat cacactgacg ttaagctggc tttcgatgct
    33901 gtatctgccg tggcctttta ctttcattat tgtgctgttg atgtggagcg ccgtgcgcct
    33961 gccacgaatg gaagcctttt tgatcttcct taccacggtg atgatggtgt cgctgatgat
    34021 ggccgcggat ccctccctgc ttgctacgcc gcgtacgtac ctgatgagcc atatgccgtg
    34081 gctaccgttt ttgctgatcc tgctgcccgc caacatcatg accatggtga tgtatgcctt
    34141 tcgtgcggaa cgcaaacaca tttccgaaag cgaaacccgc tttcggaacg cgatggaata
    34201 ttccgctatc ggtatggcgt tagtgggcac cgagggacaa tggctgcaaa ccaacaaagc
    34261 gctctgccag tttctcgggt acagtcagga agagctgcgc ggactcacct ttcagcaact
    34321 gacctggccg gaggatctca ataaagatct ccaacaggtt gaaaagctga taagcggtga
    34381 aataaacacc tattcaatgg aaaaacgcta ctacaaccgc aatggcgatg ttgtctgggc
    34441 gttgcttgcc gtctcactgg tgcgccacac ggatggcacg ccgctctatt ttatcgctca
    34501 gattgaagac attaacgagc taaaacgcac cgaacaggtc aatcagcaac tgatggagcg
    34561 catcacgctg gctaacgaag cgggcgggat tggcatctgg gagtgggagt tgaagccgaa
    34621 tgtttttagc tgggataagc ggatgttcga gctgtatgaa attcctccac atatcaaacc
    34681 gaactggcag gtgtggtacg agtgcgtgct gccggaagat cgccagcatg ccgaaaaagt
    34741 gattcgtgat tcgttgcaat cacgctcgcc ctttaaactg gaatttcgca ttaccgtaaa
    34801 agacggtatt cgccatatcc gcgccctcgc caaccgggta ctgaataaag aaggcgaagt
    34861 cgaacgtctc ctcggcatca atatggatat gaccgaagtg aaacagctta acgaggcatt
    34921 gtttcaggaa aaagagcgcc tgcacattac gcttgattcc atcggtgaag ccgtggtctg
    34981 tattgatatg gcgatgaaaa ttacctttat gaatccagtg gcggagaaga tgagcggctg
    35041 gacgcaggaa gaagcgttag gtgttccgct cctgacggtg ttgcatatta cttttggcga
    35101 caacggacca ttaatggaga acatttacag tgccgacacc tcacgttccg caatcgaaca
    35161 agatgtggtg ttgcactgtc ggagcggcgg cagttacgac gtgcattaca gtattacgcc
    35221 gttaagtact ctggacggca gcaatattgg ttcggttctg gtgattcagg acgtcaccga
    35281 atcacgcaaa atgctgcgcc agctgagcta cagcgcctcc catgatgcac tgacgcatct
    35341 cgccaaccgc gccagttttg agaaacaact gcgtatcctg ctgcaaacgg taaacagtac
    35401 gcatcagcga catgccctgg tgtttatcga tcttgatcgc tttaaagcgg tgaatgacag
    35461 cgccgggcat gcggcgggcg acgctttgct gcgcgaactg gcgtcgttga tgctgagtat
    35521 gctgcgctcc agtgacgtgc tggcgcggct cggcggtgat gaatttggtc tgctgctgcc
    35581 agactgcaat gttgaaagcg ctcgttttat cgctacacgt attatcagtg ctgtgaatga
    35641 ctatcacttt atatgggaag gccgggtaca tcgggtaggt gccagcgccg ggattacctt
    35701 gatcgatgac aacaatcatc aggcggcaga agtgatgtcg caggctgata tcgcctgtta
    35761 tgcctccaaa aatggtggac ggggccgggt gacggtttac gaaccacagc aagctgccgc
    35821 acatagcgaa cgggcggcga tgtcgcttga tgaacagtgg cggatgatta aagagaatca
    35881 gttgatgatg atcgcccacg gtgtcgcttc gccgcggatc ccggaagcgc gtaatttgtg
    35941 gctgatttcg cttaagctct ggagttgcga aggcgaaatt attgatgaac aaacatttcg
    36001 tcgtagcttc agcgatccgg cacttagcca tgctcttgac cgacgggtat tccacgaatt
    36061 tttccagcag gccgccaaag cggttgccag taaaggcata agcatcgccc tccccctttc
    36121 cgttgccggt ttgagtagcg ccacgctggt gaatgatctg cttgagcagc tggaaaatag
    36181 ccctctacca ccacggttat tacatctgat tattccggct gaagcgattt tagatcacgc
    36241 agaaagcgtg caaaaactgc agctggcggg atgtcggata gtgctcagcc aggtgggccg
    36301 cgatctgcaa atcttcaact cgctgaaagc gaatatggca gattacctgc tacttgatgg
    36361 tgagttatgc gccaacgtgc agggtaattt gatggatgag atgctgatta cgattattca
    36421 ggggcacgct cagcgactcg ggatgaaaac catcgccggg ccagtcgttt tacccttagt
    36481 gatggatacg ctttctggca tcggcgtcga tctgatttat ggtgatgtga ttgccgatgc
    36541 ccaaccgctg gatttgctgg tgaatagcag ttatttcgcg attaactgag agagcgtttg
    36601 cccattgcct gatgcgacgc taacgcgtct tatcatgcct acaaatcgct cattccccag
    36661 accggataag gcgctcgcac cgcatccggc gaccaacgtc atgcttcgtc tggttgccag
    36721 ccttccgtat accagatatg caacagcgca taagaacgcc agggcttcca gcgctcggca
    36781 taacggcgga tttgtgccgg tgtcattccc ggaaatcgcc gtttaatcag ataatcatcc
    36841 ggcagaaaaa catctttcgc ctgccagcca cgcaaagcaa aataattcgc cgtccagcgc
    36901 ccgatacccg gaaaagtttg cagcgttttc atcgcctgct ccacatcgcc cggaattgtc
    36961 attggtaagc tgccctccag cgccgcattt gccagatgaa tcagtgcctc tgcccgtttc
    37021 aacggcatgc ctaacgcttt taatgcctgc gggtcggctg ctgccagccg ctgaggcgtg
    37081 gggaagcaga tatactccgg aaaatcatcc agccgttcgc cataaagctg taccactttg
    37141 gcagtcaatt ttgccgccat cgccacgctc accagttggc ctaaaatcgc ccgcacaccc
    37201 tgctcaaaag catcaataca gccgggtaaa cgcaatccgg gccgccccgc gcctaacttt
    37261 cccaacgcac cgttaacaat ctgcgggtta cattgcagat caagcaggcg gctcattttc
    37321 gccagacact ctgcggcaac aggttctaaa cctgcactta aatttatgtg cagagtatgg
    37381 cgggctatat ccggaatagc agtcactacg ccgcgatatt cgcccaccgc cagactacgg
    37441 gcataataat tgtccgcgac cgtttccaca ccgctcaccg cacgggcggc gagaaatccc
    37501 aacatccacg accagtcata cggcggctgc cagttcaggg tatacatcgc atctccttgt
    37561 taatccgctt tcagcataaa cgttattcag acgttacgct ttgctttcat attccggttg
    37621 tcgcgacagc aacatttcgc taaagtcacg ccccttcttc accggcatgg ggattaattc
    37681 gtgtttattg gttttgatta cggtacagca aactgttcag tggcggtcat gcgtgacggt
    37741 aaaccgcaat tactgaaaat ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg
    37801 ccaacgcgtg aatcggtaag cgaatggctg taccgccatc atgatgttcc ggcagacgac
    37861 gatgaaacgc aggcgctgtt acgtcgggcg attcgttata accgcgaaga agatatcgat
    37921 gttacggcga aaagcgtgca gttcggtctt tcctcactgg cacagtacat tgatgatcca
    37981 gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg
    38041 cagcaggtag cgctgtttga ggatctggtc tgcgcaatga tgttgcacat tcgccagcag
    38101 gcgcaggcgc agctgccaga agcgattact caggcggtga ttggtcgccc gatcaacttc
    38161 caggggctgg gcggcgatga agcaaacgcc caggcgcaag agattctgga acgcgcggcg
    38221 aagcgtgccg gattcaggga cgtggtattc cagtacgagc cgatcgcggc tgggctggat
    38281 tacgaagcca ccttgcagga agaaaaacgg gtgctggtgg tggatatcgg cggtggtacg
    38341 actgactgtt cattgctgct gatggggccg cagtggcgtt cgcgtctcga tcgtgaagcc
    38401 agtctgctgg gtcacagtgg ttgtcgtatt ggcggtaacg atctggatat cgccctggcg
    38461 tttaaaaacc tgatgccact gctgggtatg ggtggcgaaa ccgaaaaagg catcgccctg
    38521 ccgatcctgc cgtggtggaa tgcggttgcc atcaacgacg tacctgcgca gagtgatttc
    38581 tacagtagtg ccaacggtcg tctgcttaac gatctggtac gcgatgcccg cgaaccggaa
    38641 aaagtggccc tgttacagaa agtctggcgt cagcgtttaa gctatcgcct ggtgcgtagc
    38701 gcagaagagt gcaaaattgc tctttcaagc gtagcggaaa cccgcgcctc actgccgttt
    38761 atcagcaatg aactggctac gctgattagc cagcgagggc tggaaagcgc cctcagccag
    38821 ccactggcgc ggattctgga acaggtgcaa ctggccctgg ataacgccca ggaaaaaccg
    38881 gacgttatct acctgaccgg cggtagcgcc cgttctccgc agattaaaaa agcgctggca
    38941 gaacagttgc cgggcattcc gattgcaggc ggcgatgact ttggctccgt caccgccggg
    39001 ctggcacgct gggcggaagt ggtgtttcgt taattgaaaa atggctcagc agcggcttaa
    39061 atctgcctgc gcctgagcca gtttttctgc tgcctgacgt aacggttcca tcaacggcat
    39121 ggtccgtgct ggagcatgaa ggcggaatcg ctgcaactct gtcgcgccga ctgtcaacgc
    39181 cgattccagc cgatgtcgtt tggcggtcat tttcgccatt accgcttgtc tgtctgctgg
    39241 cgttatcgct tcattcggcc tgaacacaaa acggcgttca cagttcgctt tccagtcgat
    39301 aaccgcctgg gtcagatggt cgccaaaccc tttcacttgc ttaacgccac gacgggtaac
    39361 atctgcggct gtttcaatac caaaagaccg taacgccgct ttacgcgcag ggccaacgcc
    39421 gggaatagag gcaacatcaa taaaaaatcc ctccagaaac ttctgcttct gccgttccct
    39481 tgcagtgtca tgaagtgctg ccagagcgcg tttctcttct tccggtaacc cgagaatttc
    39541 gtccttcatt ttttcgagca tcgtccgttt ggcgataaaa ccttccaggc cgcctacctg
    39601 ctggatctgg cggactaaat ggtcatagtc cattttcgca cgattaaatg cctcacggcg
    39661 ttgctgaact tctgctttgt acgctttgct tgtcaaactg ccaataatcc agatagccgc
    39721 cagcacagga accaaaataa tataacgcgg ttctgcctga aggccgcaaa gcagcagcga
    39781 taatgctgac agtgcgatct caagcagaat gatgtattcg cgccgtaaca ggcctaaagg
    39841 aagcggcctg ccagtcggtt ggaaatgatc gggtaatggc agttgcaatg ctggcggtgc
    39901 tactgacgcc atcaccatcg cccagacttt tgccagcaca aaatccccgc cggtggtaat
    39961 gacctcttcg ccgagatcaa taaaatagat aacaccttga ttatccagcg cacaccatgg
    40021 gcagtcagcc aaatgaccgg gataaacatg cattgccgaa acggtacatt tctttaactg
    40081 ttggcgtaga gaatccagtg ctgataccca cgctttagcc gtcggacgcc cggttgccac
    40141 gccactttcc gtaaacgcct gctgaaacat ggcttcaaca tcactcggta acatcgatag
    40201 cggaatcgat cgtggcggcg gttttaaacc acgtcgctga ttatctgacg cgtaggcata
    40261 acggaaatgg gtaatatccg tctccagcgc attacccgca tcagagataa gcggcacacc
    40321 agaataagga tgccggccac caaacaagac gtgaaaaatc agcaacgcaa ggccaaaatt
    40381 atcgtgattt tctgtgcgtt caaagccgac aaatgacgac aaggtttgta gctctggcgg
    40441 cgtaaaatgc gacacgccga cttcgcataa atgcagtgtg ccattggcgt taatctgaaa
    40501 ggagtcgcta tcaatcaaca ccactttgct gtcgcgacct accataaaac tgttctggtt
    40561 tacgtcaccc acgacgtgcc cgtgctcgtg aaccgtagca aaagatgaag caatattgcg
    40621 cgcaacatag agtagaaaat cccacgcaca atgagggtaa cgttgacgac gatgtgccgg
    40681 gctatagatc atatgaatcg gttctttacc agaaactttt ggcatcataa aaccgataac
    40741 ttttccgcct cgtccaccgt gaagcgttgc ctgcggccag gcgacataat tcaacaattg
    40801 cgcgtcagct gtcgcagcca taaaggcaag tttgtcctgt tttaaggcgg gtggcggcgt
    40861 gtgataaatc ttggcgacgc tatcgacaaa ctcattgata tcataaaccg cgccttcacc
    40921 gcctttgccc agttcacggc ctggcgtaac gcactcacca gtagcagtat ataaagtggg
    40981 tttcatggta accacagcgc cagtgccaac gttttatcat catccgtacg ctcgtttacc
    41041 gccgggctgg acaaaaactg ctttaacaat tcaggtaata aatcgagttg ttcctgcgtt
    41101 gctgacgcca gtccattgaa aaacggggta aagaaaggca catgaggaga attatccagc
    41161 atatttaacg ccagccgctg aataccatcc gtaaatgctg caactttatg cgcacgccca
    41221 gtgctggtga aagtttccag tctggaaacg gcatcttcgt cggtaatgaa gtgcgtcatg
    41281 ttggcatatt cacccaccat cggcgtgaga ggcaattgca gaccgtgacc caaatctacc
    41341 accacgccgc catcgccaat ctgcataatc aaggtgccgt caggcgatga aatcaatccc
    41401 agaaacgtgc aggcaaaatc gcgaactgct aatgctttgg cttctgcctc ggcaaaaagt
    41461 cgttgacgaa tagtcagtac catatttgtg gcgaggacat cattaagccc caattcaccg
    41521 tcctgtactt tttgcaccat aaacgccatc gcttcattga cggcgagcgt cgcgccttct
    41581 cctccctgcg aaacgctacc tgcgccatca gcaaggaaca tcaccagtaa tggttgctgg
    41641 tcgtttaacc agacaacctg catctggcag gcatcctgac aaggcaaatc agcactaatg
    41701 tgcgatgttc caacagcgga tgcataaacc agacgccagc tcacactgac gtccagcctt
    41761 ttggcgcttc cagcacaact tccgttcccg gcgtggagcg ggaaaccgaa cgcagcgaac
    41821 tggataacca gctaaacagt tcacggaatt gtaatccctg cagtggcaaa ggctgacgaa
    41881 cgctgatttg tgccagagtc ttcatatccg ccccctgaac accgatggaa aagaaggcaa
    41941 acttcttatc ttcttccccc tgaaacactt tgttggcggc agcctgccac tcatcggttg
    42001 gagcgccatc ggtgatcagg aaaatccacg ggcgataata cgaaatacca tttgcgcggt
    42061 actcacgttt cctctcttca accatatcca gggctttggt aatggcggcc cccataggcg
    42121 tatccccctg ggcaaacagg atgggcggga aaaagttagc ggcgctggta aagggttgtt
    42181 ccacatgcac cgggccgaac gtcacaatgc cgagttccac tctttttaaa gccagcggat
    42241 cagcaagcag ttcatcacga aaggtaacca atccggcgtt aagttcatta ataggtctgc
    42301 cgctcatcga gccagaaaca tccagcagca aaatgcaggg gcaacgtggt tccgggttac
    42361 tggcaaaatc gctggtggca aacgtgattt gttcgctcat aagaaatcct ttcttagaga
    42421 attatcgagg tccggtatca taccgattgc aataatgaaa taagagcaaa cgaagaaact
    42481 ttcagaatta ggcccgtcgg cgcagaaaat tttgctattc gcctcgcaag aacggagagc
    42541 cgtcgattga cactaagggc ggagtgacat aatttcagga gtgagggtta gggagaggtt
    42601 tccccctccc cctggtgttc ttagtaagcc tggaagctaa tcactaagag tatcaccagt
    42661 atgatgacgt gcttcatcat aaccctttcc ttattaaaag ccctcttctc cgggagaggg
    42721 tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc tgaatgcagt
    42781 ctatcgctaa cgcgtgaaca aaatagtttc atctgtgatt attgcgtaac gactcgcaaa
    42841 gaatatttgc tggcaggatc gcagactaca aagcctgcgg attgacaatc ttatcgtgaa
    42901 ggcataattt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtaag
    42961 cggggaagct tatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    43021 tattaaggcc ccttcctcgg gaggggcttt cccgtttcag cgtcccgctg aaatcgtcgg
    43081 cttacctcct ttcgccctgt atgcagtcta tcgctgactt gcggataaaa tagtttccag
    43141 tgtgattatt gcgtaacgtc tcgcaaagaa tatttgctgg caggatcgca gactgcaaag
    43201 cctgcagatt gacaatctta tcgtgaaggc atactttcag gagtgagggt agagcggggt
    43261 ttcccccgcc ctggtagcct tagtaagctg gccagctaat gactaagagc accacgatga
    43321 tgagtagctt catcatgacc ctttccttat ttatggcccc ttcctcggga ggggctttcc
    43381 cgtttcagcg tcccgctgaa atcggcggct tacctccttt cgccatgcaa gcagtctatc
    43441 gctaacgcgt agataaaata gtttcctgtg ttattactgg atgcgtgctc gcaaatgtgc
    43501 ccgtcattca gacgattccc gacagtgttt cataattcct ccatttttct cccttattgg
    43561 ctggctacac tagtatcatt ccgcgaaacg tttcaggaag agaaactctt aacgatgaaa
    43621 ggcagttata aatcccgttg ggtaatcgtg gtggttatcg ccgccatcgc cgcattctgg
    43681 ttctggcaag gccgcaatga ctcccagagt gcagccccag gggcgacgaa acaagcgcag
    43741 caatcgccag cgggtggtcg ccgtggtatg cgttccggcc cattagcccc ggttcaggcg
    43801 gcgaccgccg tagaacaggc agttccgcgt tacctcaccg ggcttggcac cattatcgcc
    43861 gctaatactg ttacggtgcg cagccgcgtg gacggtcaac tgatggcgtt acatttccag
    43921 gaaggccagc aggtcaaagc aggcgattta ctggcagaaa ttgaccccag ccagttcaaa
    43981 gttgcattag cacaaaccca gggccaactg gcaaaagata aagccacgct tgctaacgcc
    44041 cgccgtgatc tggcgcgtta tcagcaactg gcaaaaacca atctcgtctc ccgtcaggag
    44101 ctggatgccc aacaggcgct ggtcagtgaa accgaaggca ccattaaggc tgatgaagca
    44161 agcgtcgcca gcgcgcagtt gcaactcgac tggagccgca tcaccgcacc agtcgatggt
    44221 cgcgttggtc tcaagcaggt tgatgttggt aaccaaatct ccagtggtga taccaccgga
    44281 attgtggtga tcacccagac gcatcctatc gatttagtct ttaccctgcc ggaaagcgat
    44341 atcgctactg tagtgcaggc gcaaaaagcc ggaaaaccgc tggtggtaga agcctgggat
    44401 cgcaccaatt cgaagaaatt aagtgaaggc acgctgttaa gcctcgataa ccaaatcgat
    44461 gccacaaccg gtacgattaa agtgaaagca cgctttaata atcaggatga tgcgctgttt
    44521 cccaatcagt ttgttaacgc gcgcatgtta gtcgacaccg aacaaaacgc cgtagtgatc
    44581 ccaacagccg ccctgcaaat gggcaatgaa ggccattttg tctgggtgct gaatagcgaa
    44641 aacaaggtca gcaaacatct ggtgacgccg ggcattcagg acagtcagaa agtggtgatc
    44701 cgtgcaggta tttctgcggg cgatcgcgtg gtgacagacg gcattgatcg cctgaccgaa
    44761 ggggcgaaag tggaagtggt ggaagcccag agcgccacca ctccggaaga gaaagccacc
    44821 agccgcgaat acgcgaaaaa aggagcacgc tcctgatgca ggtgttaccc ccgagcagca
    44881 caggcggccc gtcgcgcctg tttattatgc gtcctgtcgc caccacgctg ctgatggtgg
    44941 cgatcttact cgccgggatt atcggttatc gcgccctgcc cgtttcggcg ctgccggaag
    45001 tggactatcc gaccattcag gtggtcacgc tctacccagg tgccagcccg gatgtcatga
    45061 cctctgccgt taccgcgccg ctcgaacgcc agttcgggca gatgtctggc ctgaaacaga
    45121 tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt gcagttccag ctaacattac
    45181 cgctcgatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc aacttgttgc
    45241 cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat ccgccgatca
    45301 tgacgctcgc cgtcacctca accgccatgc cgatgacgca agtggaagat atggtggaaa
    45361 cccgcgtcgc gcagaaaatc tcgcagattt ccggcgtcgg cctggtgacg ctttccggcg
    45421 gtcagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc ctcggcctga
    45481 ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggca aaaggtagcc
    45541 tcgacggccc ttcccgtgca gtcacgcttt ccgcgaacga ccagatgcaa tccgccgaag
    45601 agtatcgcca gctaatcatc gcctaccaga acggcgcgcc aattcgtctg ggcgatgtcg
    45661 caactgtaga gcaaggtgca gaaaacagct ggctcggcgc gtgggcgaac aaagaacagg
    45721 ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc gccgacagca
    45781 ttcggcagat gctgccacag ctcactgaga gtctgccgaa atcggtgaag gtgacagtgc
    45841 tttccgatcg caccaccaat atccgcgcat ccgtcgatga tactcagttt gaattgatga
    45901 tggctatcgc gctggtagtc atgattatct acctgttttt gcgcaatatt ccggcgacca
    45961 tcattcccgg tgttgctgta ccgctgtcgt taatcggcac tttcgcggtt atggtgtttc
    46021 tcgatttttc aatcaataac ctgacactga tggcgttaac tatcgccacc ggattcgtgg
    46081 tcgatgacgc catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa ggcgaaaaac
    46141 cgttggcggc ggcgctcaag ggcgcaggtg aaatcggctt taccattatc tcgctgacct
    46201 tctcactgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc gggcgactgt
    46261 tccgcgaatt tgctattacc ctggcggtag cgattttgat ctcagcggtg gtgtcgctga
    46321 ccctgacacc gatgatgtgc gcgcggatgc tcagccagga gtcgttgcgt aaacagaacc
    46381 gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat ggtcgtggac
    46441 tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcactcagc acgctgctgc
    46501 ttagcgtgct gctgtgggtg ttcattccga aaggtttctt cccggtacag gacaatggca
    46561 ttattcaggg cactttgcag gcaccgcaat ccagctcctt taccaatatg gcccagcgac
    46621 aacgccaggt cgcggacgtg attttgcagg atccggcagt gcaaagcctg acctcatttg
    46681 ttggcgttga tggcactaac ccgtcgctga atagtgcacg tttacaaatc aacctcaaac
    46741 cgttggatga acgtgatgat cgggtgcaaa aagtcatcgc ccgtctgcaa acggcggtag
    46801 ataaagtgcc gggcgtcgat ctcttcctgc aaccaacgca ggatctgact attgatactc
    46861 aggtcagccg cacccagtac cagtttacct tgcaggccac ctcactggat gcgctcagta
    46921 cctgggtgcc acagttgatg gaaaaactcc agcaactgcc acagctttct gatgtctcca
    46981 gcgactggca ggaccaaggg ctggtggcgt atgtcaatgt tgatcgcgac agcgccagcc
    47041 gtctggggat cagcatggcg gatgtcgata acgccctgta caacgcgttt ggtcagcgac
    47101 tgatttccac tatttatact caggctaacc agtatcgcgt agtgctggag cacaacaccg
    47161 aaattacccc gggcctcgcg gcgctggata ccattcgcct gaccagtagc gacggcggcg
    47221 tggtgccgtt aagctcaatt gccaaagttg aacagcgttt tgcgccgctt tccatcaacc
    47281 atctggatca gttcccggta acgaccatct cctttaacgt gccggataac tattcgctgg
    47341 gcgatgcggt gcaggagatt atggacaccg aaaagacgct gaatctgccg gtggatatca
    47401 ccacgcaatt ccagggcagc accctcgcct tccagtcggc gctgggcagc actgtctggc
    47461 tgattgtcgc ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac gagagcttta
    47521 ttcacccgat caccattctc tcgacgctac ccaccgcagg ggtcggcgcg ctgctggcat
    47581 taatgattgc cggtagcgaa ctggatgtga ttgcgattat cggcattatt ttgctgatcg
    47641 gtattgtgaa gaagaacgcc atcatgatga tcgacttcgc gctggctgct gagcgcgagc
    47701 aaggcatgtc gccgcgcgat gcaatctacc aggcttgtct gttgcgtttt cgtccgatcc
    47761 tgatgaccac cctggcggct ctgcttggcg cgctgccgct gatgttgagt accggggtcg
    47821 gcgcggaact gcgtcgtccg ttaggtatcg gcatggtcgg cggtctgatt gtcagccagg
    47881 tgctgacgct gttcaccacg ccggtgattt atctgctgtt cgaccgcctg gcattgtgga
    47941 ccaaaagtcg ctttgctcgc catgaagagg aggcgtaagt gaagtttttt gccctcttca
    48001 tttaccgccc ggtggcgacg attttactgt cggtcgccat taccctgtgc ggcatactgg
    48061 gcttccgtat gctgccggtc gccccgctgc cgcaggtcga ttttccggtg attatggtca
    48121 gcgcctcgct gcccggtgcg tcaccagaaa caatggcgtc ttccgttgcc acgccgctgg
    48181 agcgctcgct tgggcgcatt gccggggtca gcgaaatgac ctccagcagt tcgctcggca
    48241 gcacacgtat tattttgcag tttgattttg accgggacat caacggcgca gcgcgtgatg
    48301 tgcaggcggc gatcaacgct gcacaaagtt tgctgcccag tgggatgccc agccgtccga
    48361 cctatcgcaa agcgaacccg tcggatgcgc caattatgat cctcacgctg acatccgata
    48421 cttattcgca gggtgaactg tacgatttcg cctcgacgca gctggctccg acgatttcgc
    48481 aaatcgacgg tgttggtgat gtcgatgtcg gaggcagctc actgcccgcc gtacgcgtcg
    48541 ggctgaatcc gcaggcgctg tttaatcagg gcgtgtcgct ggacgacgta cgcaccgcca
    48601 tcagcaatgc caacgtgcgt aaaccgcagg gcgcgctgga agatggcact caccgctggc
    48661 agatccagac caatgatgag ctaaaaaccg ccgctgaata tcagccgttg attattcact
    48721 acaacaacgg cggcgcggtt cgtctgggcg atgtggcgac ggtgaccgac tcagtgcagg
    48781 atgtgcgcaa cgccgggatg accaacgcca aaccggctat tttactgatg atccgcaaac
    48841 tgccggaagc caatattatc cagacggttg acagcatccg ggcaaaatta ccggagttgc
    48901 aggaaaccat tccggcggcg attgatctgc aaattgccca ggatcgctcc cccaccattc
    48961 gcgcctcgct ggaagaagtc gagcaaacgc tgattatctc ggtggcgctg gtgattctgg
    49021 tggtgttttt attcctgcgc tcgggtcgcg ccactattat tcccgccgtt tcggtgccgg
    49081 tttcgctgat tggtacgttt gcggcgatgt acctgtgcgg attcagtctc aataaccttt
    49141 cgttaatggc gctcaccatc gctactggtt tcgtggtgga tgacgccatc gtggtgctgg
    49201 aaaacattgc acgtcatctg gaagcgggaa tgaaaccgtt gcaagccgca ctgcaaggta
    49261 ctcgcgaagt cggttttacg gtgctgtcga tgagtctgtc actggtggcg gtgttcctgc
    49321 cgctgctgtt gatgggcgga ttgccgggcc gactgttacg cgagtttgcc gtgacgcttt
    49381 ctgtcgccat tggtatttcg ttgctggttt ctctgacatt aacgccaatg atgtgtggct
    49441 ggatgctgaa agccagcaag ccgcgcgagc aaaagcgact gcgtggtttt ggtcgcatgt
    49501 tggtagccct gcaacaaggc tacggcaagt cactgaaatg ggtgctcaat catacccgtc
    49561 tggtgggcgt ggtgctgctt ggcaccattg cgctgaatat ctggctgtat atctcgatcc
    49621 cgaaaacctt cttcccggag caggacactg gcgtgttgat gggcgggatt caggcggatc
    49681 agagtatttc gtttcaggcg atgcgcggta agttgcagga tttcatgaaa attatccgtg
    49741 acgatccggc agtggataat gtcaccggct ttacaggcgg ttcgcgagtg aacagcggga
    49801 tgatgtttat caccctcaag ccacgcgacg aacgcagcga aacggcgcag caaattatcg
    49861 accgtctgcg cgtaaaactg gcgaaagaac cgggggcgaa tctgttcctg atggcggtac
    49921 aggatattcg cgttggtggg cgtcagtcga acgccagcta ccagtacacg ttgttatccg
    49981 acgacctggc ggcactgcgc gaatgggagc cgaaaatccg caaaaaactg gcgacgttgc
    50041 cggaactggc ggacgtgaac tccgatcagc aggataacgg cgcggagatg aatctggttt
    50101 acgaccgcga caccatggca cggctgggaa tcgacgtgca ggccgccaac agcctgttaa
    50161 ataacgcctt cggtcagcgg caaatctcga ccatttacca gccgatgaac cagtataaag
    50221 tggtgatgga agtggatccg cgctataccc aggacatcag tgcgctggaa aaaatgttcg
    50281 ttatcaataa cgaaggcaaa gcgatcccgc tgtcgtattt cgctaaatgg caaccggcga
    50341 acgctccgct gtcggtgaat catcagggat tatcggcggc ctcgaccatc tcgttcaacc
    50401 tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca atgacccagc
    50461 ttggtgtgcc ttcgacggtg cgcggcagtt ttgccggaac agcgcaggtg ttccaggaga
    50521 cgatgaactc acaggtgatc ctgattatcg ccgccatcgc cacggtgtat atcgtgctgg
    50581 gtatccttta cgagagttac gtacacccac tgacgattct ctccaccctg ccctcggcgg
    50641 gcgttggtgc gctgctggcg ctggagctgt tcaatgcccc gttcagccta atagccctga
    50701 tagggatcat gctattaatc ggcatcgtaa agaaaaacgc cattatgatg gtcgattttg
    50761 cgctcgaagc ccaacggcac ggtaacctga cgccgcagga agctattttc caggcctgtc
    50821 tgctgcgttt tcgcccgatt atgatgacta ccctggcggc gctgtttggc gcgctgccgc
    50881 tggtgttgtc gggcggtgac ggctcggagc tgcggcaccc cctggggatc accattgtcg
    50941 gtggactggt aatgagccag cttctgacgc tgtataccac gccggtggtg tatctctttt
    51001 tcgaccgtct gcggctgcgt ttttcgcgta aacctaaaca aacggtaacc gagtaaatga
    51061 cagaacttcc cgacagcacc cgctggcaat tgtggattgt ggctttcggc ttctttatgc
    51121 agtcgctgga caccaccatc gttaacaccg cccttccctc aatggcgcaa agcctcgggg
    51181 aaagtccgtt gcatatgcac atggtcattg tctcttatgt gctgaccgtg gcggtgatgc
    51241 tgcccgccag cggctggctg gcggacaaag tcggcgtgcg caatattttc tttaccgcca
    51301 tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc cggcacgctg aacgaactgt
    51361 tgctggcacg cgcgttacag ggcgttggcg gtgcgatgat ggtgccggtc ggcaggttga
    51421 cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc gatgaccttt gtcacgttac
    51481 ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg tctgctggtg gagtacgcgt
    51541 cgtggcactg gatctttttg atcaacattc cggtggggat tatcggtgcg atcgccacat
    51601 tgctgttaat gccgaactac accatgcaga cgcggcgctt tgatctttcc ggctttttat
    51661 tgctggcggt tggcatggct gtattaaccc tggcgctgga cggcagtaaa ggtacaggtt
    51721 tatcgccgct ggcgattgct ggcctggtcg cagttggcgt ggtggcactg gtgctttatc
    51781 tgctgcacgc cagaaataac aaccgcgccc tgttcagtct gaaactgttc cgcactcgta
    51841 ccttttcgct gggcctggcg gggagctttg ccggacgtat tggcagtggc atgttgccct
    51901 ttatgacacc ggttttcctg caaattggcc tcggtttctc gccgtttcat gccggactga
    51961 tgatgatccc gatggtactt ggcagcatgg gaatgaagcg aattgtggtt caggtggtga
    52021 atcgctttgg ttatcgtcgg gtactggtag cgaccacgct gggtctgtcg ctgatcaccc
    52081 tgttgtttat gactaccgct ctgttgggct ggtactacgt tttgccgttc gtcctgtttt
    52141 tacaagggat ggtcaactcg acccgtttct cctccatgaa caccctgacg ctgaaagatc
    52201 tcccggacaa tctggcgagc agcggcaaca gtctgctgtc gatgattatg caattgtcga
    52261 tgagtatcgg cgtcaccatc gccgggctgt tgctgggact ttttggttcg cagcatgtca
    52321 gcgtcgacag cggcaccaca caaaccgtct ttatgtacac ctggcttagc atggcgttta
    52381 tcatcgccct tccggcgttc atctttgcca gagtgccgaa cgatacgcat caaaatgtag
    52441 ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg cgacctggta ttaccggcaa
    52501 actgtttctg gcgattttcg ccacctgcat tgttttgctg atcagtatgc actgggcggt
    52561 gcgtatcagt tttgagcgtg gctttattga ttacatcaag catggtaatg aacagcgatt
    52621 acaactgtta agtgatgcgc ttggcgagca gtatgcgcag catggcaact ggcgcttcct
    52681 gcgcaacaat gatcgctttg tctttcagat cctgcgttca tttgaacacg ataattcgga
    52741 agataaaccc ggcccgggta tgccaccgca cggctggcgt acccagttct gggtggttga
    52801 tcaaaacaac aaagtgctgg ttggtccgcg agcgccgatt ccacctgacg gtacacggcg
    52861 acccattctg gtcaacggtg cggaagttgg cgcggtgatc gcctcccccg ttgagcggtt
    52921 aacgcgcaat actgatatca atttcgataa acaacagcgg caaaccagct ggttgattgt
    52981 cgccctggca acgttactcg cggcacttgc cacttttctg ctggcgcgcg gtttactggc
    53041 accggtaaaa cgacttgtcg atggcacgca caaactggcg gcgggcgatt tcactacccg
    53101 cgtaacgccc accagtgaag atgaactggg caaactggcg caagacttca accagcttgc
    53161 cagcacactg gagaaaaacc agcaaatgcg gcgcgatttt atggccgata tttctcacga
    53221 actgcgtacg ccattagcgg tgctgcgcgg tgaactggaa gctattcagg atggcgtgcg
    53281 caaattcacg ccggagacgg tggcgtcttt acaggcggag gtcggtacac tgaccaaact
    53341 ggttgacgat ctccatcagt tgtcgatgtc tgatgaaggc gccctcgcct atcaaaaagc
    53401 accggtagat ttgatcccac tgctggaagt ggcgggcggc gcatttcgcg aacgattcgc
    53461 cagccgtggc ctgaaactgc aattttccct gccagacagt attaccgtat ttggcgatcg
    53521 cgaccgttta atgcagttat tcaataattt acttgaaaac agcctgcgct acactgacag
    53581 cggcggcagc ctgaaaatct ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc
    53641 cgacagtgcg ccaggtgtca gtgacgagca gctacaaaaa ttatttgaac gtttttatcg
    53701 caccgaaggt tcccgcaacc gcgccagcgg tggttccggg ctggggctgg cgatttgcct
    53761 gaacattgtt gaagcacata atggccgcat tattgctgcc cattcgcctt ttggcggggt
    53821 aagcattaca gtagagttac cgctggaacg ggatttacag agagaagtat gaccgagtta
    53881 ccaatcgacg aaaacacacc gcgtattttg atcgtggaag atgaaccgaa gctggggcag
    53941 ttgctcattg attatctgcg tgctgcgagc tatgcgccga cgcttatcag ccacggcgat
    54001 caggtactgc cgtatgtgcg ccagacacca ccggatctga tcctgttgga tctgatgctc
    54061 cctggcaccg atggcttgac gctgtgccgg gaaattcgtc gtttttctga cattcctatc
    54121 gtgatggtga cggcaaaaat cgaagagatc gaccgcctgc tcggactgga gattggcgca
    54181 gatgactaca tctgcaagcc gtacagccca cgggaagtgg tagcgcgcgt caaaaccatt
    54241 ttgcgccgtt gcaaaccgca gcgcgaattg cagcaacagg atgccgaaag cccgttgatt
    54301 atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa tgcttgacct gacgcctgcg
    54361 gaatttcgtc tgttgaaaac gctctctcat gaaccaggaa aagtgttctc ccgcgagcaa
    54421 ttgctcaatc atctttatga cgactaccgc gtagtaaccg accgcaccat tgacagccac
    54481 attaaaaacc tgcgccgcaa gctggaatct ctcgacgccg aacagtcatt tatccgcgcc
    54541 gtttacggcg ttggttaccg ctgggaagcc gacgcctgcc gcatcgttta gttttagcga
    54601 catgactttg ttagccggag atggcgttca gaccagatcc ggcaacatta tcccacgcat
    54661 ggtcagcaaa ctgac
//
LOCUS       DAZOYS010000006        48488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOYS010000006 DAZOYS010000000
VERSION     DAZOYS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.006"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            175..1392
                     /gene="alaA"
                     /locus_tag="ACHUN9_000268"
     CDS             175..1392
                     /gene="alaA"
                     /locus_tag="ACHUN9_000268"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0693787.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHUN9_000269"
     CDS             1476..2075
                     /gene="yfbR"
                     /locus_tag="ACHUN9_000269"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0693788.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2134..3966)
                     /locus_tag="ACHUN9_000270"
     CDS             complement(2134..3966)
                     /locus_tag="ACHUN9_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0693789.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4053..4701)
                     /gene="hxpA"
                     /locus_tag="ACHUN9_000271"
                     /pseudo
     CDS             complement(4053..4701)
                     /gene="hxpA"
                     /locus_tag="ACHUN9_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4712..5206)
                     /locus_tag="ACHUN9_000272"
     CDS             complement(4712..5206)
                     /locus_tag="ACHUN9_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0693790.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5289..5744)
                     /gene="yfbV"
                     /locus_tag="ACHUN9_000273"
     CDS             complement(5289..5744)
                     /gene="yfbV"
                     /locus_tag="ACHUN9_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0693791.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6082..7284
                     /gene="ackA"
                     /locus_tag="ACHUN9_000274"
     CDS             6082..7284
                     /gene="ackA"
                     /locus_tag="ACHUN9_000274"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0693792.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7359..9503
                     /gene="pta"
                     /locus_tag="ACHUN9_000275"
     CDS             7359..9503
                     /gene="pta"
                     /locus_tag="ACHUN9_000275"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0693793.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9693..11213
                     /gene="yfcC"
                     /locus_tag="ACHUN9_000276"
     CDS             9693..11213
                     /gene="yfcC"
                     /locus_tag="ACHUN9_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0693794.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="ACHUN9_000277"
     CDS             complement(11246..11788)
                     /gene="yfcD"
                     /locus_tag="ACHUN9_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0693795.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="ACHUN9_000278"
     CDS             complement(11846..12397)
                     /gene="yfcE"
                     /locus_tag="ACHUN9_000278"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0693796.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="ACHUN9_000279"
     CDS             complement(12453..13097)
                     /gene="yfcF"
                     /locus_tag="ACHUN9_000279"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0693797.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13233..13880
                     /gene="yfcG"
                     /locus_tag="ACHUN9_000280"
     CDS             13233..13880
                     /gene="yfcG"
                     /locus_tag="ACHUN9_000280"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0693798.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13937..14299
                     /gene="folX"
                     /locus_tag="ACHUN9_000281"
     CDS             13937..14299
                     /gene="folX"
                     /locus_tag="ACHUN9_000281"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0693799.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14320..15213
                     /locus_tag="ACHUN9_000282"
     CDS             14320..15213
                     /locus_tag="ACHUN9_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0693800.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15261..16151)
                     /gene="rpnB"
                     /locus_tag="ACHUN9_000283"
     CDS             complement(15261..16151)
                     /gene="rpnB"
                     /locus_tag="ACHUN9_000283"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0693801.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16348..17121)
                     /gene="hisP"
                     /locus_tag="ACHUN9_000284"
     CDS             complement(16348..17121)
                     /gene="hisP"
                     /locus_tag="ACHUN9_000284"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0693802.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17129..17845)
                     /locus_tag="ACHUN9_000285"
     CDS             complement(17129..17845)
                     /locus_tag="ACHUN9_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0693803.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17842..18528)
                     /gene="hisQ"
                     /locus_tag="ACHUN9_000286"
     CDS             complement(17842..18528)
                     /gene="hisQ"
                     /locus_tag="ACHUN9_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0693804.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18618..19400)
                     /gene="hisJ"
                     /locus_tag="ACHUN9_000287"
     CDS             complement(18618..19400)
                     /gene="hisJ"
                     /locus_tag="ACHUN9_000287"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0693805.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19621..20403)
                     /gene="argT"
                     /locus_tag="ACHUN9_000288"
     CDS             complement(19621..20403)
                     /gene="argT"
                     /locus_tag="ACHUN9_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0693806.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20669..21238)
                     /gene="ubiX"
                     /locus_tag="ACHUN9_000289"
     CDS             complement(20669..21238)
                     /gene="ubiX"
                     /locus_tag="ACHUN9_000289"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0693807.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21333..22850)
                     /gene="purF"
                     /locus_tag="ACHUN9_000290"
     CDS             complement(21333..22850)
                     /gene="purF"
                     /locus_tag="ACHUN9_000290"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0693808.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22887..23375)
                     /gene="cvpA"
                     /locus_tag="ACHUN9_000291"
     CDS             complement(22887..23375)
                     /gene="cvpA"
                     /locus_tag="ACHUN9_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0693809.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23634..24296)
                     /gene="dedD"
                     /locus_tag="ACHUN9_000292"
     CDS             complement(23634..24296)
                     /gene="dedD"
                     /locus_tag="ACHUN9_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0693810.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24286..25554)
                     /gene="folC"
                     /locus_tag="ACHUN9_000293"
     CDS             complement(24286..25554)
                     /gene="folC"
                     /locus_tag="ACHUN9_000293"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0693811.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVSQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25624..26538)
                     /gene="accD"
                     /locus_tag="ACHUN9_000294"
     CDS             complement(25624..26538)
                     /gene="accD"
                     /locus_tag="ACHUN9_000294"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0693812.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26694..27353)
                     /locus_tag="ACHUN9_000295"
     CDS             complement(26694..27353)
                     /locus_tag="ACHUN9_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0693813.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27436..28248)
                     /gene="truA"
                     /locus_tag="ACHUN9_000296"
     CDS             complement(27436..28248)
                     /gene="truA"
                     /locus_tag="ACHUN9_000296"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0693814.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28248..29261)
                     /locus_tag="ACHUN9_000297"
     CDS             complement(28248..29261)
                     /locus_tag="ACHUN9_000297"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0693815.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29327..30463)
                     /gene="pdxB"
                     /locus_tag="ACHUN9_000298"
     CDS             complement(29327..30463)
                     /gene="pdxB"
                     /locus_tag="ACHUN9_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0693816.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30562..31557
                     /gene="flk"
                     /locus_tag="ACHUN9_000299"
     CDS             30562..31557
                     /gene="flk"
                     /locus_tag="ACHUN9_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0693817.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31554..32732)
                     /locus_tag="ACHUN9_000300"
     CDS             complement(31554..32732)
                     /locus_tag="ACHUN9_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0693818.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33007..34227)
                     /gene="fabB"
                     /locus_tag="ACHUN9_000301"
     CDS             complement(33007..34227)
                     /gene="fabB"
                     /locus_tag="ACHUN9_000301"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0693819.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34386..36392
                     /gene="mnmC"
                     /locus_tag="ACHUN9_000302"
     CDS             34386..36392
                     /gene="mnmC"
                     /locus_tag="ACHUN9_000302"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0693820.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36513..36791)
                     /locus_tag="ACHUN9_000303"
     CDS             complement(36513..36791)
                     /locus_tag="ACHUN9_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0693821.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36825..37373)
                     /gene="epmC"
                     /locus_tag="ACHUN9_000304"
     CDS             complement(36825..37373)
                     /gene="epmC"
                     /locus_tag="ACHUN9_000304"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0693822.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37373..38182)
                     /locus_tag="ACHUN9_000305"
     CDS             complement(37373..38182)
                     /locus_tag="ACHUN9_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0693823.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38182..39006)
                     /gene="mepA"
                     /locus_tag="ACHUN9_000306"
     CDS             complement(38182..39006)
                     /gene="mepA"
                     /locus_tag="ACHUN9_000306"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0693824.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39010..40095)
                     /gene="aroC"
                     /locus_tag="ACHUN9_000307"
     CDS             complement(39010..40095)
                     /gene="aroC"
                     /locus_tag="ACHUN9_000307"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0693825.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40130..41062)
                     /gene="prmB"
                     /locus_tag="ACHUN9_000308"
     CDS             complement(40130..41062)
                     /gene="prmB"
                     /locus_tag="ACHUN9_000308"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0693826.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41228..41779
                     /gene="smrB"
                     /locus_tag="ACHUN9_000309"
     CDS             41228..41779
                     /gene="smrB"
                     /locus_tag="ACHUN9_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0693827.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41901..42773)
                     /locus_tag="ACHUN9_000310"
     CDS             complement(41901..42773)
                     /locus_tag="ACHUN9_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0693828.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42760..43284)
                     /locus_tag="ACHUN9_000311"
     CDS             complement(42760..43284)
                     /locus_tag="ACHUN9_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0693829.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43281..43751)
                     /locus_tag="ACHUN9_000312"
     CDS             complement(43281..43751)
                     /locus_tag="ACHUN9_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0693830.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43748..44296)
                     /locus_tag="ACHUN9_000313"
     CDS             complement(43748..44296)
                     /locus_tag="ACHUN9_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0693831.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44271..45022)
                     /locus_tag="ACHUN9_000314"
                     /pseudo
     CDS             complement(44271..45022)
                     /locus_tag="ACHUN9_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45042..47684)
                     /gene="yfcU"
                     /locus_tag="ACHUN9_000315"
                     /pseudo
     CDS             complement(45042..47684)
                     /gene="yfcU"
                     /locus_tag="ACHUN9_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47766..48329)
                     /locus_tag="ACHUN9_000316"
     CDS             complement(47766..48329)
                     /locus_tag="ACHUN9_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0693832.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12030 a        13036 c        11971 g        11451 t
ORIGIN      
        1 gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc gagccttccc
       61 ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag tacattgttc
      121 taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag attcatgtcc
      181 cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg tccggtgctg
      241 aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa catcggcaac
      301 ccagccccat tcggttttga cgcgccagat gaaatcctcg ttgacgtgat acgcaacctg
      361 cctaccgctc aagggtattg cgattccaaa gggctttact ccgcgcgtaa agccatcatg
      421 cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta catcggcaat
      481 ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg ggacgaaatg
      541 ttggttcctg caccagatta cccactctgg accgcggcgg tttcgctttc cagcggtaaa
      601 gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga tgatattcgc
      661 gctaaaatta cgcctcgtac gcgtgggatc gttattatca acccaaataa cccaaccggc
      721 gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca gcataatctc
      781 attatcttcg ccgatgaaat ttatgacaaa attctctacg acgatgctga gcatcactca
      841 attgcgccac tggcacctga cctgctgacc attaccttta acggactgtc gaaaacgtac
      901 cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa acacgccaaa
      961 ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa cgttcctgcg
     1021 caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt tattacccct
     1081 ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga tattccgggc
     1141 gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga cgccaaacgc
     1201 tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga aaaagttctg
     1261 ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat tgtcacgcta
     1321 ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct ttctggttat
     1381 catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagcgtt atcccgccac
     1441 aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat ttttttgccc
     1501 atctctcccg cctgaaactc attaaccgct ggccgctaat gcgcaacgtg cggacggaaa
     1561 atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca gctatcaaaa
     1621 atcgaaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg atgtaccacg
     1681 atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc aattcgcaaa
     1741 tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc gatatggttc
     1801 cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat agcgatgaag
     1861 aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt ctggaagaac
     1921 tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag
     1981 cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt
     2041 cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag
     2101 gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat
     2161 caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca caaagtcact
     2221 gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa ccggtgtcat
     2281 aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg acacccccat
     2341 cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagagat
     2401 aaatagccca ataaccgccg acaagacaaa caaacacccc agcatcatat gtggcccgta
     2461 accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa
     2521 tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt
     2581 ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg cgatgatagc
     2641 ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca ccagacagaa
     2701 aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt
     2761 cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac
     2821 aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac
     2881 gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac caatttcgcg
     2941 caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc
     3001 aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact
     3061 acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc
     3121 gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc
     3181 aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca gccgttgacc
     3241 aatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc
     3301 acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg tctgggtatc
     3361 ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca gcacgaccag
     3421 gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca gcaattcact
     3481 gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctta tcagcccggc
     3541 aaaactcaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg caacgcttaa
     3601 caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc
     3661 gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcaa ctttgaccag
     3721 ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa taataaacaa
     3781 ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg ggactgtcag
     3841 cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca tacgcactct
     3901 gcccgtcgca aacaagacaa tggcaacggc cagtaatgaa agaacccaaa tcaattcacc
     3961 gttcacaact tatccttgtt aattgaggga gatgtcttga ttctgccata aaaaagcccc
     4021 gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc gccatttggc
     4081 tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc attcaggcgc
     4141 ggggtatctg ccggagcgtt aaccgcaatg acatgacaac ccgccgccag gccagaaagc
     4201 acgccagcgg agcatcttcc accaccacac actcctgcgg cgcaagcccc agcagctgcg
     4261 cgcctaacag atacgcatca ggttctggtt ttccgcgctt cactcgctca gcggttacaa
     4321 acacctctgg tgcgggaagc ccagctattt tatggcgcgc tcgcgctacc ggcatggagc
     4381 cagaagtcac aatggcccac ggaatacctg ctttattcaa atgactgagt aaggcgattg
     4441 cccccggaag cgggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa
     4501 ctcggcggca atatcagcct cggatttgcc cgccataaaa tggcgcagag aggtgatcgc
     4561 ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca
     4621 gttgctccac gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa
     4681 cagaaaacct ttgcaccgca cgcgggcctc ctcaggcatt gataatttga ttaatttcgt
     4741 tggcgctcaa atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt
     4801 tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc
     4861 gcccttccac attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt
     4921 caaagccgag gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc
     4981 aggaaacatg caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt
     5041 ctttcagctc gccaaactcg cgatccaatt cacgcatctg taatccgtaa cctcgctcaa
     5101 taattgtttg caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct
     5161 tgtactgatt agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta
     5221 tcactctact acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca
     5281 ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc
     5341 gtgtcagcta atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac
     5401 tcctgcaatt taccgcgaac ttcataaaac cagttaagga ttgcaggggg taatggcgtt
     5461 acagaacgct tgcccagcca ccacaatccc tgcatgggta aacttaaggc gaacagtgca
     5521 gtggcaactg ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg
     5581 gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca
     5641 aagaccgggg caaggcgttt ttccagtggc cacgtctttg agtaatgctg tccgcggcga
     5701 aacaagctaa aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc
     5761 acatataaag attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca
     5821 ttttgtcttc catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca
     5881 tattcattga gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg
     5941 gcatagactc aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat
     6001 cataaatgtc ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac
     6061 gtatcaatta taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt
     6121 tcttcactga aatttgccat cattgatgca gtaaatggtg aagagtacct ttctggttta
     6181 gccgaatgtt tccacctgcc tgaagcacgt atcaaatgga aaatggacgg caataaacag
     6241 gaagcggctt taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact
     6301 attctggcac aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta
     6361 cacggcggcg aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc
     6421 aaagatgcag cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa
     6481 gctctgaaat ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc
     6541 caccagacta tgccggaaga gtcttacctc tacgccctgc cttacaacct gtacaaagag
     6601 cacggcatcc gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg
     6661 gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac
     6721 ggtggttccg tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc
     6781 ccgctggaag gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc
     6841 cacctgcacg acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag
     6901 tctggcctgc tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac
     6961 gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac
     7021 atcggtgcct acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt
     7081 atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc
     7141 tttgaagttg atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac
     7201 aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa
     7261 gacgcgagcc gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg
     7321 taacccgcca aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga
     7381 tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg
     7441 aacgcaaagg cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg
     7501 atgcgcccga tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg
     7561 aaccgctgaa aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga
     7621 tggaagagat cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg
     7681 aaggtctggt cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta
     7741 aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc
     7801 tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca
     7861 ccggcgttat cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg
     7921 atctgtccga gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga
     7981 agctgcaaga atccagccct ctgccggttc tcggcgctgt gccgtggagc tttgacctga
     8041 tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg
     8101 acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc
     8161 tggagcactt ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg
     8221 tggccgcttg cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg
     8281 gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc
     8341 cggtatttat ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc
     8401 tggaagttcc ggttgacgat cacgaacgta tcgagaaagt tcaggaatac gttgctaact
     8461 acatcaacgc tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt
     8521 ctccgcctgc gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg
     8581 tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg
     8641 gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc
     8701 agggtgtaga actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct
     8761 atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg
     8821 aacagctgga agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg
     8881 gtctggtttc cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga
     8941 tcaaaactgc accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac
     9001 aggtttacgt ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag
     9061 aaatcgcgat tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta
     9121 tgctctccta ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag
     9181 caactcgtct ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg
     9241 acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc
     9301 gcgctaccgt gtttatcttc ccggatctga acaccggtaa caccacctac aaagcggtac
     9361 agcgttctgc cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta
     9421 acgacctgtc ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga
     9481 ttcagtctgc acagcagcag taacctcgtc atcatccgca gctttgcgct gcggatatct
     9541 gaaccggaaa taatcactat ttccggtttt ttattctctt aatctgcatt aatcctttct
     9601 gattatcttg cttaactgcg ctgcatcaat gaattgcgcc atttcacttt acatacttac
     9661 cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa
     9721 caagaagatg ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca
     9781 gccttgccac ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg
     9841 ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta
     9901 acgaagcagg cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc
     9961 cgggcctgat gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagctg
    10021 ttggcatcat catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag
    10081 gaaccattga taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct
    10141 ttattcctgc gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag
    10201 aggccgtcgc ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta
    10261 ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga
    10321 acccgttttg tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg
    10381 ggttgcgcat cgtggtgtgg gttatcgcca ctatgattgg cctgatcttt accatggtgt
    10441 acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct
    10501 ttcgtgaaaa gcaggcggat gttgaacaac gtccgtttac ctttggtgac tggctggtat
    10561 tgattgtcct gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt
    10621 ttattccaga aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg
    10681 ttgttttccg ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg
    10741 cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg
    10801 tcggtaatgg tgaagcgggt gatgccagcg tgttaaatac catcctcaac agcattgcca
    10861 atgccattag cggtctggac aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg
    10921 tatttaattt cttcgtgacg tccggttctg gtcaggcggc gttaaccatg ccgttactgg
    10981 caccgcttgg cgatctggtc ggtgttaacc gtcaggttac cgtgctggca ttccagtttg
    11041 gtgatggctt cagtcacatc atttacccaa cctcagcttc gttaatggca actctcggtg
    11101 tgtgcagggt ggacttccgt aactggctga aggtgggcgc gaccctactt ggactgctgt
    11161 ttattatgtc cagcgtcgta gtgatcggcg ctcagttgat gggctaccac taaaaatgtt
    11221 aagagccgca attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt
    11281 tggcattgcg cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc
    11341 agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta
    11401 gggcgaaggg accgtgagag acgcagctga acaacgcgcc ccagacacgg caatttttat
    11461 cttcgaaata gaactgcccg tgctcggcaa aggggacacc ggcaatgccc aactcttctt
    11521 ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg
    11581 tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt
    11641 tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca
    11701 tttgttcccg gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt
    11761 cagtacttgc caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta
    11821 cggcgcattt tgaattgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt
    11881 gatcattgag tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat
    11941 taccgccttt cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt
    12001 ctgccactgg cagatgggta tgaccgtaca ccagcacatc gttctggttt aaagcaggta
    12061 gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata
    12121 cctgttgcca cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc
    12181 agttgccgcg cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg
    12241 ctggcgcgta accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga
    12301 tcaccagcca ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta
    12361 acgacccatg aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac
    12421 gtaagctatg atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca
    12481 ataaaacgct ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct
    12541 ggcacctcat cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca
    12601 atgcaccatt caccaaataa attcggctga cccagtgcta acaaatgttc tgccatcgcg
    12661 aacagtttct ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca
    12721 aagacaacat ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc
    12781 tgaatccgtc gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc
    12841 ggtggcgcaa atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa
    12901 tcatcgattt gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggcttc
    12961 aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc
    13021 aacgccaccc aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg
    13081 atagcgggtt tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag
    13141 cctttttgtc actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt
    13201 ggtgttcaga tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac
    13261 ccaatggtca caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta
    13321 aggtagacct ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca
    13381 acaaaattcc ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ctaagcctct
    13441 ttgagtctgg tgccattttg ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg
    13501 aaacgcgtga gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc
    13561 cgatgcttgg gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta
    13621 ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt actaaacaag cggctggaaa
    13681 actcgccctg gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg
    13741 ttaatgcctg gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc
    13801 atgagcggat ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg
    13861 atgagcgttc ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa
    13921 caacggagaa cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt
    13981 acgtacgttt atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa
    14041 tgtgacgatc cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct
    14101 gaattatcgc accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt
    14161 gttggaaaaa ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta
    14221 tgctgaagtg gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac
    14281 cttaagctgg cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga
    14341 gggacgggat taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc
    14401 acggtagtga cgcgtaaccc acagaaagcc agttccgttc tcggccctcg ggtgacgcta
    14461 tggcaagggc tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc
    14521 ggagaaccga ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc
    14581 cgctggaata tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccgccgtcg
    14641 gtactcattt ccggctcggc aacgggctat tatggcgact taggtgaagt ggtggttacc
    14701 gaagaggaac cgccgcataa cgaatttact cataaactct gcgcccgctg ggaagagatt
    14761 gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc tgcgaaccgg cgtggtactg
    14821 gcaccggacg gcggtattct cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg
    14881 ccgattggtt ccggtcggca gtatctggcc tggattcata ttgatgatat ggtcaacggc
    14941 attctctggc tgctggataa cgacctgcgc gggccattta atatggtttc gccctacccg
    15001 gtacgcaatg aacaatttgc ccatgcgctc ggtcaagcgc tgcatcgccc ggccattttg
    15061 cgcgtccccg caaccgccat tcggctgtta atgggcgaat cttcaatact ggtattaggt
    15121 ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ctggtacgat
    15181 ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt tacagcaaac atccgccagt
    15241 taactcccgg tgttacagga ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg
    15301 gtcaccatca taactaactc tctgtctaaa cctctatcca gcatctcctg agcaatacgc
    15361 agggcttctt cgtgtttgcc ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc
    15421 atcagtttct ccttttcttg tggtgcgcgt tctgttatct caccaataaa tgcacggaaa
    15481 cgctgggcat cccctgtttg taatacgtaa ttaaacaggg cttttagctg tctgtcatta
    15541 gtgttccctg taactagcag cgaaacaatt tggtcgacta atcccaacag atcgcgctga
    15601 cgaatatgtt tctgaattaa ctccaacagc gccattttgc ggtgttgcat aatctcgtca
    15661 tccggcacca cggtaatatc caccaacgga aacgctgatg aatatatttt gcgggctata
    15721 gcaggctcgg caaattcatc aagccagcag agtgaataag gataaggact tctgcaacca
    15781 tgataaaaca gcatcgggat caccaatgga agctctttat agcccgcatc aagatggttt
    15841 tgcattgccg caatggaata acgcatcatg cgaaaagcca ttaattcttc cagcttactt
    15901 tggtgctcta tcactacata aatataaccc actccctcct gcgttttcac agaccacaag
    15961 aggtcggaat aatattgccg caggtcttca tcaataaaac tgtttgattc tagtttaagc
    16021 gtcgttaaat cacacagttt gcgcagcggc gcgggaagat gaatatcaat aaaatcccgc
    16081 gcggtgtctg gatggcgtaa aaaagattta aataccgcat catgcggcgt ggaagttgtc
    16141 gatattgtca tggcgttccg tcacccctca aatttaagat gacgcgacaa taaccacgat
    16201 taacacccaa tgcagcaggc agtttttatc tttggacagc gaattcagaa gtatttttag
    16261 cggactgcaa cttttacatt tcagcggaag ctgactcgca aattccccat gaaaatactc
    16321 cggcagcgtt agccaccgga gaggaaatta tttcagcgat cccttaagga actgttgcag
    16381 acgagggctt tgcgggttgc caaataactg ctccggcgcg ccctcttctt ctattttccc
    16441 ctgatggagg aaaatgacgt gagtagaaac atggcgagca aagcccattt cgtgagtcac
    16501 taccaccatg gttttcccct cttctgccag ttgctgcata atacgtaaca cttcgcctac
    16561 cagttcagga tcgagcgccg aggtaggttc atcaaacagt aaaacttccg gttccatcgc
    16621 cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg gaaagatgca ccggatattt
    16681 cccctgcgca cgttcgtcta tcccgacttt cgccagatac ttcaccgccc gctcgcgcgc
    16741 ttcctgcttg ctcaggccca acacctgaat cggcgcttcc atgacgtttt ccagcaccgt
    16801 catatggctc cagagattga agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg
    16861 cagttgattt ttatcggcga ctttgagttg accgtctttg tcgcgcacca gattgatcgt
    16921 ctggccattg accacgatcg acccttcact cggtttttcg aggaagttaa tgcagcgcag
    16981 aaaggtactt ttccccgatc ccgacgatcc gatgatgctt attacatctc cggcattcgc
    17041 ttgcagtgag acccctttca gcacttcatg ttcgccgtag cgtttgtgca aatcgataac
    17101 gtttaattta ttctcggaca tcgtattctc agtgcgttga agaaggtttc acatgctgca
    17161 accagcgttt ttccgctctg cgaaagaggc tgatcaggac ataagagatg attaaataga
    17221 gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt ggcggcgtta atatcgcggg
    17281 ctattttcag cagatccggc acagtggccg taaacgccag cgcagtagag tgcagcatca
    17341 ggatcacttc gttgctgtat gccggtaacg caatacgcag cgcagaaggc aaaataatgc
    17401 aacgatacat tttaaaagtc gagaagccat aggctctggc ggcttcaatt tccccatgcg
    17461 gaaccgaacg gattgcccca gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca
    17521 gcgccagcac ggtacagttc aggccactgc ggaagaaagc gttaaggaat tcggttccct
    17581 taacaatctc aagcgtgtac atgccggaat agaacaccag caactgaaca tacagcggcg
    17641 taccgcgaaa aatataggta aataaccaga ttggaaactg gatgtattta ttactggaga
    17701 cacgaccaat cgccagaaac agcgccagga ctccgcctat cactaccgac aaaataagca
    17761 gccacagagt gatcgccaca ccagtaaagc gataaccgtc ggtccacagc agcggtttcc
    17821 agtattcatg taagatttcg atcacaggtc agccctcttc acacccacgg agtagcggcg
    17881 ctcaaggaac agcagcacac cattggaaac agtggtgaaa accaggtaaa tcacgccaca
    17941 gacgatggcg aaatagaacg gttcccaggt acttttgcct gccagttggg tggctttgac
    18001 cacatcttcc aggccgagta acgaaaccag tgcggtagat ttgaggatca cctgccagtt
    18061 gttgccaatg cctggcaggg cgtaacgcat catcgccgga aacatgatcc gccgaaacac
    18121 ttgcccacga gtaaaaccga acgccgtcgc cgcctctata tgtcctttcg gcactgccat
    18181 aaaagcacca cggaacgttt cggtaaaata agcaccgtag ataaaaccga gagtgataat
    18241 accagcgacc atcggatcga tatcaatctg cccgacgccc atcgcctccg tcaccgtgtt
    18301 tagcgcaatc tgcaaaccgt agaaaatcag cagcatcaac actaagtccg gcacgccacg
    18361 aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc cgattttgcg agagcttacc
    18421 gccagcgcca attaaaccga tgattacagc gagcactaca gagctgatag ccagctccag
    18481 cgtgacgagc gcaccctgta aaataacacc tgaaaaccca tacaacatgc tgcctgtcct
    18541 gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt tacccgaagg gagcgggctt
    18601 tctttcaccg atggcgatta gccaccataa acatcaaaat cgaagtactt tttcgctaat
    18661 ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt tgttcagtgc ttcgcgcagt
    18721 tcgttatctt ctttacgcag gcccatgccg gtccctacgc caaacagttt ttcatcttta
    18781 acagacgggc caccgaattt gtaatcttta ccgacaggtt gtttgaggaa accttcgctg
    18841 gcagcgacct catcctggaa cgcggcatca atacgtccgg cagtcaggtc agaataaatg
    18901 ttgtcctgcc cctgatacga gacgatttca atgccttttg gtgcccaatg ttcattaccg
    18961 aacgtctcct gggtggtgcc ctgcaatacg cctacccgtt tgcctttcag cgactcgact
    19021 gtcggctgaa tgtcagaatt tttcgccacc accaaacgag aatcggcagc gtacagtttg
    19081 tcggtgaagg ctatttcttg ctgacgtttt tccgtaatgg aaagcgatga catgatggcg
    19141 tcaatcttct tcgcttttaa ggacgggatt aacgcatcca gcggattttc gacaaacgta
    19201 cattgcgtat tgatgcgttt gcataattcc tttgccagat cgatatcgaa gccaaccagt
    19261 tcgccttgtg aattttttga ttcaaatggc gcataggtcg ggtcggtacc gatgcggatg
    19321 ttttgcggaa tcgcagcaaa tgccgcagtt gcgctggaga aggccagaac cagagagagc
    19381 gatagcacca gttttttcat aatgtcctca actgacagtc tttttatagg gaatatttac
    19441 aggtttgtta cacttatcgt gccataaaat tgaccatcaa ggcaaaatat tccgccccgt
    19501 tagacgtatt atttttcatt aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg
    19561 caacaaacct ggcgtgcacc atgatgatca ttccatcagg tacagcttcc cagcgacgta
    19621 tcagtcaccg tagacattaa agtcgaaata ctttttcgcc atcttgtcgt aggtgccgtc
    19681 ctgacgcagc tcgccaagcg ccttattgaa ggcagccgtc agttcagcat catctttacg
    19741 tagccctaca ccggtgccat caccgaagta ttttttgtct tttactgatg agccagcaaa
    19801 ggcgaaagct ttaccagcag gttgcttgag gaatccttcg ctggcagcaa cttcatcttg
    19861 taacgcagca tccagacgtc ctgcagccag atcggaatag accaaatcct ggttggcata
    19921 ggccaccaca tccacgcctt tgctacgcca ggtctcgtta gcgtaagctt cctgggttga
    19981 tccctgcagc acaccaacat gtttaccttt cagtgaatcc agcgttggct gaatcggtga
    20041 acctttggca gcaatcaaac gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc
    20101 ctgctgacgt ttatcggtaa tggaaagcga cgaaataatg gcgtcgattt ttttcgcttt
    20161 cagtgagggg atcagcgcgt caaagtcact ggcaacccag gtacatttca cctgcatccg
    20221 tttgcacatc tcgttaccga gatcgatatc aaagccaaca aaatcacctt tagcatcttt
    20281 cgacgagaac ggcgcatagg tggtatcggt tccgatacgt accgtctccg gtagcgccgc
    20341 atagctggaa gccgctgcgg agagaccgac taacaaagac agagcgagaa tcgacttctt
    20401 catacataac cctcaagtga aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc
    20461 aggctctttc atgcaggtct tatgccatct tgacgacaac agtaacattc aacgttaaat
    20521 atgttaataa gacgttgcat tattgtcctg aagttgaaga tagcaggtat agcggttgaa
    20581 tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa
    20641 attgccctga aacagggcaa cagcggagtt atgcgccctg ccagcgggca aagagatctt
    20701 caggaagggt tattgcaaac tggtcaagaa cacgattaac cgtctgattt atcacatcat
    20761 caagggattg cgggcgatga taaaacgccg gaacgggagg cataatcacc gcaccgattt
    20821 ctgccgcctg agtcattaaa cgcagatggc ctaagtgcaa tggtgtttca cgcacgcaga
    20881 gcaccaacgg gcgacgctct ttcagcacca catctgccgc acgggtcagt aagccatcag
    20941 tatagctatg gacaatgccg gaaagggttt tgattgaaca gggtaaaatc accatcccca
    21001 gcgtctggaa agaaccggaa gagatgctgg cggaaatatc gcgcgcatcg tgcgtgacat
    21061 cagctaatgc ctgcacttcg cgcagggaaa aatccgtttc gagggataag gtctggcgcg
    21121 ccgcctggct catcaccaga tgcgtttcga tatctgtgac atcgcgcaga acctgtaata
    21181 agcgcacgcc ataaatcgcg ccgctggcac cgctgatgcc tacaatgagt cgtttcataa
    21241 atagttgccc tgtcagactt gcaggcagac tttgcagtat ttcggcggga gttgcaagtc
    21301 agggtgccag accggcaccc tcagcgaagg catcatcctt cgttatgcat ttcgagattt
    21361 tccacttcgt tctgacgttg cactgctttg gcgtcatcat tacgtaacgt atcgaggaaa
    21421 tcgaggtagc cctgatcaac atctttggtg acgtagacgc cgttgaacac cgagcattca
    21481 aactgctgga tatccggatt ttcagcgcga acggcgtcga tcagatcgtt cagatcctgg
    21541 aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat caacttcgcg accgtgagcg
    21601 atcagttccg tggcgctcgg catatcaata ccataaacgt tcgggaagcg aatttccggt
    21661 gccgcagaag cgaggtacac tttcttcgct ccggcttcgc gtgccatctc gataatctgc
    21721 tcagaagtgg tgccacggac gatggagtcg tcgaccagca ggacgttttt atcgcggaac
    21781 tcggcgcggt tggcattcag tttacggcgc acggacttac gacgcagctg ctggcccggc
    21841 atgataaagg tgcggccaac atagcggttt ttaacgaagc cctggcggta cggtttgccc
    21901 agaatacgag caatttccag cgcgatatca cacgaggttt ctgggatcgg gatcaccacg
    21961 tcgatatcca gatcttccca ttcgcgggca attttctcgc ccagtttcgt gcccatattc
    22021 acacgcgcgc tgtaaacgga aattttgtcg atgaacgagt ccgggcgagc aaagtataca
    22081 tactcaaaca ggcacggatt gctgaccgga ttgtcagcac attgacgggt aaacaactgc
    22141 ccttcttcag tgatgtaaat cgcttcgccc ggcgcgacgt cacgcaggaa atcaaagccc
    22201 agcgtatcga gcgctacgct ttcggaagcg accatatatt ctgtacggtt ctcgtcaata
    22261 tcacgttttc ccagtaccag cggacgaatc ccgtttggat cgcggaaagc aaccataccg
    22321 tggccgataa tcatcgccac acaggcatat gcgccgcgga ttaagcggtt tgtggcagca
    22381 atggcagcga aaatattgtc ggcttccagc gggtagtggc ggaagttgtc cagctcgcta
    22441 gcgaagatat taagcagaat ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct
    22501 tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta
    22561 atgccatacg gggagttaac gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc
    22621 gtagggtaac gcacatgacc aatgcccata ttgccctgca aacgctgcat atggcgagct
    22681 tcaaatacat cgctcaccag cccgttcgct ttacgcaaac ggaagcagtt attggcatct
    22741 atggtgatga tgccggcggc atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa
    22801 atcgactggt taaccggcat aacaccggcg ataccgacaa taccgcacat acgtcttttt
    22861 cctcgttaag ccacatctca gagcacttac gctctgggca agaaacttga cgagctttgc
    22921 agataatcaa aaaaccatct gatgataaaa ctgaactgcg ggatcagctg tgatttgctc
    22981 cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa agaagagaat ggcggcaaca
    23041 atcaacacac cgcgcaacgc accgaaacag acgcccagca cccgatcggt gcctgacaac
    23101 cccgttttct ccaccaactg gcctatcacg aagttcacga tagcaccaac aatcagggta
    23161 gcgataaaca gtaccgcgat ggcaattcca tttcgaacca gttcgtcttc aaagcccgta
    23221 aaccagactg acaggtaagt gtagtaatga ctggcaacaa agaaagcaca accccatgtc
    23281 accagcgata acgcttcacg aacaaagccg cggatcaggc taaccagaga ggaaaaagca
    23341 atcaccgcga ttatggcgta atcaatccag accatatgtg tcccacgatt ttacgccctg
    23401 tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg tttgcgtagg gatttccttc
    23461 ccacgcatca ataaaaatgg cgctgaaaaa atattcaacg ccatcgactt tttatgcctt
    23521 tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc cttatccgac ctacggttct
    23581 aacactgccc aggcctgata agacgcgcca gcgtcgcatc aggcaagact gtattaattc
    23641 ggcgtatagc ccattaccac gccacttaag ccagaaagtt gcttcaactc acccagcgaa
    23701 cctttcagct tatctttcga ggcatccggc ccaaccagaa tacgggtaat tttaccctgc
    23761 actggcgtgg atggcgacgt ataaacccga taaccggcac cgcgcagctt accgacaatc
    23821 tcattcactt tatcggcatt tttcagcgca cccagttgca caacataggc tttacccgtt
    23881 ggcgctgctt tttcttccac gaccggcttc ggttctggct tcggcgcagg tggtgcttca
    23941 accttcggtt taggcggctc caccggtttc ggcttcggtg gggcgaccgg tgcaggttcc
    24001 ggttcaaact cggtgttatt ggctgcaata gtggccggat cgagcgacgg tgctgcggca
    24061 tcacctgccc gcacctcttc cgctgcgcct tccggcggct gcgtcggcaa cgcctgggtg
    24121 gcggctggca tcatatcagg ctcatcgcga tcgcccgctt ttggaaccag cgggatagcc
    24181 gcaaactcat cctgataatg ttttttctgc ccgtccagca gccctggaag tacaatcacc
    24241 cccagcgcca ccagcacgat cgtgcccact aaccgattct gaaacttact tgccaccgct
    24301 tctcctcgcg tcaatcactt ccatgacatg tgcgaccgtg tggaaagaac cacacaccag
    24361 cacggtgtct tccgctttag cgtccgccat tgcggcatcc catgcctgcg caacgctatc
    24421 aaatgatttg ccgttaccca aatgctcaag cagttgttct gccgtggcac cgcgcggccc
    24481 ttccagtggc gcacaatacc agtcatcaac cacgcttttc aaccaggcca gagttccggc
    24541 aatatcttta tcatgtagca taccgataac cgccagcacg cgcccgtttt tcggtagcgc
    24601 tttcatacgc ccggtgagat attccgccgc atgtggatta tgcgcgacat caaaaataac
    24661 gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa attgcgctgg caatcccgtc
    24721 gcgaatggca ttttcactga cttccagccc gctggcacgc agtgccgcca gcgctgttgc
    24781 ggcattcggt tgcgagacaa gcggcaacgg cagattttcc agcgtgccgt gagcatcgct
    24841 aaacgcccag tcatgatcgg tgacggaata gttccactca acgccccgac gttgtaacag
    24901 tgcacctttt tcctgcgcca catcagcaat ggtagaaggc atttccggct caccgacaat
    24961 tgccggtttt gcgctgcgaa agatgcctgc tttctcgcga ccaatacttt cgcgatctgg
    25021 tcccagccag tcggtatgat ccagcgcaat gctggttact acagccacat cggcatcgac
    25081 aatattggtt gcgtccagac gaccgcccag ccctacttcc agaatcacta cgtcaagttg
    25141 tgcctgtttg aacagccaca acgccgacag cgtaccgtac tcgaaatagg tcagggaaat
    25201 atcaccgcgt gccgattcaa tctccgcaaa agaggcggtg tgggccgatt ccggcaattc
    25261 ctgcccctgc acacgtacgc gctcggtata acgcaccaga tgcggtgaac tgtagacgcc
    25321 cactttgtac cctgccgcca tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc
    25381 attcgtaccc gcaacggtaa acacaaatgg cgctggtttc aggacgccaa gacgcgccgc
    25441 gacctggctc acgcgctcaa ggccgagatc gatagtttta ctgtgcaggt tttccagata
    25501 agaaagccac gaagccagag gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc
    25561 cgctgattcg ttacggtgag aatagcaaaa gggcagagcc agtggccctg cccttatcag
    25621 ttatcaggcc tcaggttcct gatccggtac cgggggtacc actacgcctt cacgcggcgc
    25681 ttcaggattc ggcgctggca gattcatcaa cttcgccaga atgctcgcca gtttcaggcg
    25741 catttccgga cgatggacga tcatgtcgat ggcgcctttc tcgatcagga attcactgcg
    25801 ctggaatcct ggcggcagtt tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa
    25861 gccaattaac gctttcggtt cagcgatgtt gagatcgccc agcatggcga aacttgcaga
    25921 aacaccgccc atcgtcgggt cggtcagcac ggagatgtac ggcaagccgc gctcctgcat
    25981 ttttgccagt gccgcagagg ttttcgccat ctgcatcagc gacatcagtg cttcctgcat
    26041 acgtgcgcca ccagaggcgg agaagcagat cagcgggcag ttatcttcca gcgcctgctc
    26101 aacggcacgc acgaaacgtg cacccacaac agaccccatt gaaccgccca taaaggcgaa
    26161 ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg cctttcatca ccaccagcgc
    26221 atctttttcg ccggtttctt tctgcgcaga agccagacgg tctttatact tcttggagtc
    26281 acgaaacttc agcacatctt tcggctcaag ctcgctaccc agctccacaa ggcttccttc
    26341 atctaacagg ctatgcaggc gattacgcgc tgtcatacgc atgtgatggt cacacttcgg
    26401 acagacctca agattacgtt ccagctcagc gcggtataaa acctgaccgc agctatcaca
    26461 cttagtccac accccttcag gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat
    26521 tcgttcaatc cagctcatta gggacctttc tgtctgaacc tggttcgatg ccagttttat
    26581 ctttggggac gcataatgcc atttttgccc ccaacagacc atgaatgttg cacattaaaa
    26641 cataacagcc cgaaactttg gataaaaaag tggtcgaacc gcggagttac tttttatttt
    26701 gcggcgcgtg ccgcagcgcg tttgtgacga ataaattcga aaaccccagg caatattgaa
    26761 acaaaaataa tgccaacaat catcagctta aggttgctct gaatgaaggg gagcgtgccg
    26821 aagaaataac ccgcgtaggt aaaaatcagt acccacaaca gtgcgccgat cacgttataa
    26881 gcggcgaaat gacggtacga catgtggccc attcccgcaa caaacggcgc gaacgttctg
    26941 acgatcggta caaaacgggc gagaataatc gttttgccgc catgtctctc gtaaaactga
    27001 tgagtcttat caagataact gcgacgaaag attttcgaat ccgggttact gaacagtttg
    27061 tcaccaaata accgtccaat ggtatagttt actgcatcac cgacaatagc ggcaatcaac
    27121 ataagcacca ccatcatatg gacattgaga tcgttagttt ccagcgacgc caatgcacca
    27181 gcaacaaaca gcagcgaatc accaggcagg aatggcgtta ccaccagccc cgtttcgcag
    27241 aacagaatca aaaacagaat ggcataaacc cagacgccgt actccgcgac cagctctgcc
    27301 agatgcacgt caatgtgcag gataaaatcg atgaggaaat aaatcaggtc catatttgcc
    27361 tatgccttgt actcgtcatc tttcaggctg taactacgtt ggctacagcc tgaaatgttc
    27421 cgagcattat tctcgttagt ccgccagaaa tagcgggccc attggcggtt ttggaagatc
    27481 ataccggtca gggtaatcca ccgcgaccag atacagccct tccgcttttg ccgttgctgc
    27541 cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc catctctccg gctggttgtg
    27601 ggcaccgact tccatcaggc tgccgacaat attcctgacc atatgatgta caaaggcatt
    27661 cgctttgata tctaccacca cataaggacc gtgacgcgtg acattaatat gcataacgtt
    27721 gcgccacggg gttcgggact ggcactgcac cgcacggaac gaggtgaaat cattctcgcc
    27781 cagcaagcat tgcgcagccc gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt
    27841 taccccttta ctcaataccg ccgggcgcag ccgatgattg tagatgatgt agcgataacg
    27901 gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt acagctttaa cccaacgcac
    27961 ggcgatgtca ccaggtaaat tcgcatttac gcccaaggtc cacgccgcgt ctttgcgctg
    28021 cgcggttgtt tcgaaatgca ccacctgccc ggtaccgtgt acccctgcgt cagtacgccc
    28081 ggcgcagaag acggtgatgg gttcgttcgc cacctgggag agcgcctttt ccagcttctc
    28141 ctgcacactg cggacttcat tctgccgttg ccagccgtaa tacttactgc cgtcgtactc
    28201 aatgcccagc gcaattttat aaactggcag ttgttgctgg tcggacatta gtacagatac
    28261 tcctgcacca gtttctcggc gatttttact gccatcagcg cgccgccaaa gcgaacgtta
    28321 tcggccaccg accagaactg gacttgctcc ggcataccgt agtcattacg cacgcagcca
    28381 acagaaagat gcggcgtacc cgaagcatcc cctacctgag tcgggaattc gttctcttca
    28441 gagagcacaa tatcttcgcc ctgagcaaac gcatcacgcg cttcttctgc cgccagcgga
    28501 cgcagtgctt caaagttgac catctgggca tgaccgtaga ataccggtgc ctggacgacg
    28561 cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc gtacttcgtc aacgatacga
    28621 cgttcttcac gcacgctacc ttcgctatcc ggcaacaacg gcagcatatt gaacgccagt
    28681 tgacgaccga agaaatcttc ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc
    28741 cccgctaacg catcgaccgc ttttttgccc tgggcggagg ctgaaatcag gctggtaaca
    28801 ctgatacgcg acaaaccgcc ctgatcgatt aacggtttca gtgccgccag cagctggctg
    28861 gtcagactgt ctggtacggc gatgacattc cggttgcggt aatcggtcag tacaaacggg
    28921 tttacttccg gcaccaccag cggtacgtcg ggttcgagag caaacagtcc gctgctgtcg
    28981 atcaccaggc aacctgagtt ggtcgcttct tcaacccagg cagcggtagc ttctttgcct
    29041 gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt cagcggcatc ctgcacagtg
    29101 attgttttac caccaaagcg cagttgttcg cctgcgcttt cgttacgtgc cagtgcataa
    29161 atttccccaa ccgggaactg acgttcagcc agcgtttcaa gcagggcttc gcccacagcg
    29221 ccagttgcgc ccaggacggc aatgttccag ccttcagaca tggtggttta ctccagaaat
    29281 agcaaagctc cctgccaatg ttacagcaga gagcatgaag aagagattaa cgtgccggat
    29341 gatgaacggc gttaaaaccc agtttacaca gcaatgatgc cgcactggcg tcatcacaaa
    29401 ttacatacag agatgaccat tcacggcgct caagatagtt tttgcgcagt ttatcgaact
    29461 cacccggtat cccggcgact ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca
    29521 aatgcaccag ccttttcagc gtcggttgat cgagcgggcc atgcagcgta atgcgaccaa
    29581 actctggcgc gggcagtaat gtatccagcg caacgtgctg ttcatgccca ataaacttgc
    29641 tataggcttc aaacacttgc gtagtaccgc gtgctttacc ttccagggta taacctgcga
    29701 tatgcggcgt gccgatatcc acttttgtca gcaactccac gttaagttcc ggttcgcctt
    29761 cccagacatc cagcactacg cttaacttct ggccttcatt caggcaggtc agcaacgcag
    29821 tattatcgac gactgcgcca cggcaggcgt taatcagaat cgctcccggc ttcaggctac
    29881 ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg cccatctttg aagagtggcg
    29941 tatggaaagt cagaatatcc gcatgctgaa ctaattcatc cagcgagcgg aaatcaccct
    30001 catccccacg gtcagcgcga ggcggatcgc aaagcaatgt tttaatccct aacgcttcca
    30061 gtcgcgcctg caatcgacgt ccaacgttac caacgcccac gatccccacc gtacggtcgt
    30121 gcagtgaaaa tccatcgcgt tcggcaagca tcagtagtga ggagaaaaca tattccacca
    30181 ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg
    30241 cttcatcgac atggtcggtc cccgcagtgg cagtgccaac aaatttaatg ggttttcctg
    30301 ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca
    30361 gttgagcgac ggggattgga cgcccgggaa ccgcggtcac ctcacccaaa cggctaaata
    30421 agtcgcgggc ataaggcata ttttcatcaa caaggatttt cacgtttgtg ttacctgtat
    30481 gagacgagag ttaaccggac aagtgtgcca taatctcgcg gccaggcata cttgcgaaga
    30541 tttcaggtat aaggatacgt aatgatacaa cctatttccg gccctcctcc tgggcaacca
    30601 ccaggtcagg gagataacct gccgtctggc gcgggcaatc agcctttatc cagtcagcaa
    30661 cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac tgacgcaaca acaaagagca
    30721 gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg gagattcacc gctgctttcg
    30781 cgtcacttcc ctgccgctga gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc
    30841 cattctgccc gccagctttt agcgcaatta ggggagtatt tacgtctggg gaataatcgt
    30901 caggcggtca cggattatat ccgtcataac tttggtcaga cgccgctgaa tcagctctca
    30961 ccggagcaat taaaaaccat tctcaccctg ttgcaggaag ggaagatggt tattccgcaa
    31021 ccacagcagc gcgaggcgac cgaccgtcct ttattaccgg cggagcacaa tgcgctaaaa
    31081 cagctggtga ccaaacttgc ggcggcaacg ggggaaccca gcaaacagat ctggcaatcg
    31141 atgctggaac tttccggggt gaacgatggc gagttaattc cagcgaaact gtttaaccat
    31201 ctggtgacct ggttgcaggc gcgccagacg ttaagccagc aaaatacgcc gacgctggaa
    31261 tcactacaga tggcgctaaa acaaccttta gatgccagtg aactggcagc gttatcggcg
    31321 tatatccagc aaaaatatgg tctttctgcg caatcatcgc tttcttctgc ccaggccgag
    31381 gatattctta atcagcttta tcaacggcgg gttaaaggga ttgatccgcg tgatatgcaa
    31441 ccgctgctta atccttttcc accgatgatg gacacgttgc aaaatatggc aacgcgtccc
    31501 gcgctgtgga tactgttagt cgcgattatc ctgatgctgg tctggctggt tcgttagccc
    31561 cgacgaaatg acagtatcgt gacaataatt cccagcaccg cagagatcgc cccggcaaga
    31621 aataccgaag agtaaccaaa cgtggtcgcc agcattcccg ccagcggccc ggagacgccg
    31681 agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg
    31741 acgcgtttaa ccacctccac gcccagcgca ggaaagataa gcgaacatcc ggctccggtt
    31801 aacgccgcgc ccgctaatgc gacccacgca cctggggctt gccagagcag caacaagccc
    31861 accgtttcta caagcagaga gacaatcgcc actttcacgc cgccaaaacg gtccggcatc
    31921 cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa actcggtaag agtaaagccc
    31981 gccatcgccc atcctttgct ggcaaagtag agcgaaacga aagtcccgat aaccgcaaaa
    32041 ccaacgcctt gtagtgccag ccctaaccct ggtttccaga taaacccgac aacgctccac
    32101 agcgatggac gttctcccgc cagggccggt actttgcgca ctgtgccgtt acaggcccac
    32161 gccagtaagg gtaataccat tgtggtgatc gccagtgcgg caaaaccgta atggctatga
    32221 atcaacaggc caagcggagc accaacagcg agggcaccgt aaatcgccat tccattccat
    32281 gacatcactt tgccagagtg ttttggccct acgatgccta acccccaggt cagagcgcct
    32341 gtcagtaact ggctttcacc aaacccaaga atcaaacgcc cgatgaccaa cagggcaaat
    32401 ttgaacggtg cggagacagg caaaatcgcc gccagcagca acgcgccgcc agccagacca
    32461 caagctaaca tcccctgaag cgccgaacgt tttgcgccat attgatcggc cagtcgcccg
    32521 gcatagccac gcgtcagcac cgtagccaga aactgaatcc cgacggcaat gccgaccatg
    32581 gtattgccat agcccagttc atgatgaaca aacagcggga taaccggcaa cggcagccct
    32641 acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga agagcgaaaa attggcagaa
    32701 gatcgtgttt cggtttggct tacagcagtc atgcattact ccagaatgca gcgcaaggcg
    32761 aggagtatcc ccgtctcatc tctctggttt cagggttacg gtgcgttggc aggatttaac
    32821 gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt ttgcctggaa ctggtggcga
    32881 ttgtcaatga tgtgaaaaag ggaaccatca ggttcccttt tgcgttagtg ccggaggccg
    32941 cattggcgcg taacgtcgga tgcgacgctg gcgcgtctta tccgacctac tccggcctac
    33001 tgcgaattaa tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa
    33061 gctgttagac ataacagtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc
    33121 cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag
    33181 catcagcaga gagtagatag cttcctgcac acccgcagcg cccagtgagt gaccggtcat
    33241 ggctttggtt gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag
    33301 ctctttcacg tcgccaaccg gagtcgaagt accgtgagag ttcaggtaat cgattggggt
    33361 atcaacgcca tgcatcgcca tctgcataca acgtactgcg ccttcgccag acggagcaac
    33421 catgtctgca ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc
    33481 acgcgccagc gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac
    33541 gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt
    33601 agtagacagc gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc
    33661 gccgccagca aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat
    33721 acagtgtgcg gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa
    33781 cggcgtggcg aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc
    33841 aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg ccgaatacct ggaaacgcgg
    33901 ggaaccgccg ccagaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg
    33961 agaaaggccc gcatctgcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc
    34021 gctcataaag cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc
    34081 ccagacgtgg ctacgcatgc cggaatcctt cagctcctga gagaaagtga tccctgaacg
    34141 tccttcacgc agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc
    34201 caggccagta atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt
    34261 ttcgaatgca caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga
    34321 gaaatttgcg cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa
    34381 cttacgtgaa acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac
    34441 ctgtttcccg agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc
    34501 gttatgtttt tctgggaggc aaccaattag aggtacgctt tcctgagcat ccacatcctc
    34561 tgtttgtggt agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagg
    34621 catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta catttcatta
    34681 gttttgagaa atttcccctc acccgtgcgg atttagcctt agcgcatcaa cactggccgg
    34741 aactggctcc gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc
    34801 atcgtttatt gctcaatgaa ggccgcgtga cgctggattt atggtttggc gatattaacg
    34861 aactgaccag ccaactggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg
    34921 gctttgcgcc agctaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa
    34981 ggttggcgcg tccaggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg
    35041 gtttgcagga agccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa
    35101 tgctttgtgg ggtgatggaa cagacattac cactcccctg ctccacgccg tggtttaatc
    35161 gtacgggcag cagcaaacgg gaagtagcga ttatcggcgg tggtattgcc agcgcgttgt
    35221 tgtcgctggc gttattacgg cgcggctggc aggtagcgct ttattgcgcg gatgaagccc
    35281 ccgcgctggg tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg
    35341 atgaggcgct aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc
    35401 aattacccgt taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga
    35461 aaagccagca taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag
    35521 ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta
    35581 cttatccgca aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg
    35641 cgcaacagca gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg
    35701 atgactgttg gttgttgaat tttgcaggag atcagcaagc aacacacagc gtagtggtac
    35761 tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc gactctcccg gtgtattcgg
    35821 ttgccgggca ggtcagccat attccgacaa cgccggaact ggcagagctg aagcaggtgc
    35881 tgtgctatga cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg
    35941 ccagttatca tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc
    36001 gccagcggtt gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg
    36061 ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca
    36121 atgttcccga ttatgaggca acactcgtgg aatatgcgtc gttggcggag cagaaagatg
    36181 aggcggtaag cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg
    36241 gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga
    36301 ttccgatgga tgccagtacg ctgacggcgt taaacccgaa tcggttatgg gtgcggaaat
    36361 tgttgaaggg taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg
    36421 gcgtgaacgc cttatccgac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt
    36481 cgcatcaggc atggtgctcc agacgccgca acttactgct gtgacgcctg ctgaaacagg
    36541 ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg
    36601 gctttttcca gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca
    36661 ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac
    36721 tcatcatcac tggcatggtc aaccatgccg tcgattaatg ccagaatgcg tgattcaaac
    36781 tccgcgatca tcttctttcc tcattgtaaa acgtggcacc ggcttcagtt gagcgcttcc
    36841 ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta
    36901 atgaagcgtg ccggacgctc gggaatgcca tttgtcagat aatccatcac ctgcgcatgt
    36961 acccgccgtt gaaacacaac acgatccggt tcgaagtcgc cttccagatt gtcgcaactg
    37021 acgttaaacg gatatcccgc cgccacacag aacaaccagt cgagtgcctg cggcttcact
    37081 tcaacatctt caaactggct ttgcgtttgg gcatcacgcc catccgggca ataccagtag
    37141 ccgaaatcaa ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga
    37201 attgcgctgg cataaaagcc gtgggcaaag acaattcggt tatacggtac ttccgcatca
    37261 gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca
    37321 aagcagctat taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca
    37381 tccccaacca gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca
    37441 ccgccgagac gataacaatc atcgggcgga tcagcttttg tcctttgctc aacaccagcc
    37501 gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa
    37561 tcactttgcc gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta
    37621 atttggcgtg agccgtggct ttggcgaggt tgaatccgca cagcgtaacg aaggccagcg
    37681 cgtaaaacga tccggcagcc gggccaaaga atccgtcata aaaaccgacg cagccaccgg
    37741 caattaacgc gaagggtaaa ccgtacatcc ggcgctggcg atcttcttca cccagctttg
    37801 gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga
    37861 catcagcctg aacgtactgt accaacagtg cgccgctcat tgagccgaca aaggtcatgg
    37921 caatattgag tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg
    37981 cggaaataga gccgccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca
    38041 ttcccgcagc catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga
    38101 taaatcccgc cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa
    38161 acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg
    38221 cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cccggtttta gaggtgcaaa
    38281 ccagctttgc agttctgccc cgcaaccatc gcctgatggc ggtaaaggtt gatcttcaca
    38341 ctccagacta tcggcagggc aacgtaatcg tacatgcata tgcgcgcgat gctggaacca
    38401 gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt
    38461 aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcagcga gtttgatcaa
    38521 gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa
    38581 gtccagtgct tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag
    38641 aaagatatcg acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc
    38701 agcgggcatc cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct
    38761 actcagacgc tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt
    38821 acgcatgacc tgataatgtt cggactgtat cggcaacgtg tcagcgccga caatacagcc
    38881 attagaaaaa ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca
    38941 cggcgtcgct gccaggctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt
    39001 gttcattttt taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg
    39061 taacaggtga tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat
    39121 cccgacacag ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt
    39181 aatagtgcgc cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat
    39241 ttgctgcccg ctgctgatac cgccgagaat gccgcccgca tggttgctct ggaaaccgtc
    39301 tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa aaccgtcgcc
    39361 aatttccacg cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag
    39421 gcggtcaaag accggctcgc caagtccggc ggggacacca ctggcaacaa cggtgacttt
    39481 cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc agttcatcta acgcgtcgat
    39541 tttgtccggg tccgggcaga agaacggatt ttgttcaacc agcgaccagt ctttgatttc
    39601 cagcggaatg tcgcccatct gggtcaagca gccgcggatt tcaataccaa atttctcggc
    39661 gagatatttt ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga
    39721 acgtccaccg ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg
    39781 gcctgggcgg aaaacgtctt taattgcgct gtaatcctga gagcgctggt cagtgttttc
    39841 gatcaacaag ccaatgctgg tgccagtagt aacgccttca aaaacaccgg agagaatttt
    39901 gacctgatcc ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacggtc
    39961 gaggtcatgt tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat
    40021 gcagccgagc gccagcccat gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat
    40081 tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat
    40141 aaatcgcgaa atattcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat
    40201 cgccgccgtt atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat
    40261 gtaccatgct gttgccgact tcacaaatca acacgccatc atcagcaagg taatctgccg
    40321 cgtttccaag aatgcgacgc gtcagtttaa ggccgtcggt gccagatgcc aggcccagtt
    40381 ccggctcgtg acggtattcg tttggcaggt cggacatatc ttccgcgtcg acatacggcg
    40441 ggttagtgac aatcagatcg tactgcactt tcggcaaatc acggaacaga tcggaacgaa
    40501 tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcggca accgccagcg
    40561 cgtcgggaga gatatctacc gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa
    40621 tggcgatgca gccgctaccg gtacacatat ctaaaatatg ctgcggttgc ttgctaatga
    40681 gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt
    40741 catcgacgta aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa
    40801 tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg gtgtttttcg ctggaggtca
    40861 gacgcgcggt gcgcatatct tccgggatat ccagcggcag gtagagcgat ggtaacacca
    40921 gctgtacggc ttcatcccac gggttatcgg tgccgtgacc gtaccagata tttgccgcgc
    40981 tgaagcggct taccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt
    41041 catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc
    41101 tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac
    41161 gtttaaaacg atcgattgtg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga
    41221 cagataaatg aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt
    41281 gatggcgggg actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa
    41341 aatcagcgaa gtgccggtga aacgcttgat ccaggagcag gctgatgcca gccattattt
    41401 ctccgatgag tttcagccgt tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga
    41461 tgtcagccat tttgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt
    41521 ggatttacac ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc
    41581 ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga agcatatttt
    41641 gaagcaacaa acaccgctgt ggctggcaca acatccgcat gtgatggcat ttcaccaggc
    41701 accgaaagag tacggcggtg atgcggcttt gttggtgttg attgaagtgg aagagtggtt
    41761 gccgccggag ttgccctgaa tgctggcagc aaatgccgga gcagcgtaaa cgccttatcc
    41821 ggcctacaaa tatgtgataa ttcaataaat tgcaattcac ttcgtaggcc tgataagacg
    41881 catcagagca atactacgaa ctacagttca ttcaatacat actgaaaacg acacgtcgaa
    41941 tcaatcggtt gcccactact ggtattcaca ttcaccacaa tatcgtgtag tactttatcc
    42001 gttaccggat gacgagttgg cgtaaccgtt accgttgatt tatcacaacc gtaagaggta
    42061 aaggttaaca attctgatac cgcgcggcag tatgctcctc tgccatcatt atcagaaaaa
    42121 ttactgccat cacagagtct gccgccttcc atcaggtagg tcgaaagcgt ggtggtcact
    42181 gttgaccccg tggactgaat tcgtaacgcc ttattatggc tgacataatt ttcactccag
    42241 gagatatcgt atgaagtgac cggtatcgtt gggcatattg tgttatgcac tgtaggggcc
    42301 gtatagcttc caaatccggg taaatctgta taggaataaa aggatgcgat ccaagggcaa
    42361 ccataatcat cagatgcaac ggtaatttta aacgtcatat atgcacaacg aaaaattttc
    42421 gtatctatgc cagggcaatt tgagttatta attgtatcaa agtcagtcag taccaaagtt
    42481 ggagctgaac ccgacccaga ccaggaaata acccgcaacg tatcgccttt atacgcaaaa
    42541 gaatcatatt tcccatatgt aacctccttt ggcgttatca ccgtaacatt gaaaacactg
    42601 ctcgctacag caccggttac cttgtccatg gcataataaa ctctcacact gtcaaaactc
    42661 gcactcataa gtttatctcc agcataactc ttagatgcgc cgacaacact cagaacgagc
    42721 agcattaggc tcgcgaaaat tatggtccat tttttcattt cagaaatact caaaggttgc
    42781 cgtcactgtg gcactaaagt ctccggacgt tacatctcgc ccgctctttg cctgtaacca
    42841 ggtattaaaa tttaggctgt tatttccctg attcagcggg agcgtgacgc ctgttgtatt
    42901 gttgattggc accgatgtgc ctgccgcagt ttcaatacca atcccagcgc cggatgctga
    42961 tgacgaggag tcaaaggcca gaaagccggg tagagcacta tcttctgttc ctgtcaacgt
    43021 tactttgaca ttaaacaatg tagaactatg acaatctttc aattgaaaca ccaatggcac
    43081 tcgctctgac tgcccaaggt tcattaaatc gctggcggca atggcaggaa aatgtacttc
    43141 tgccaactgg ctaccattaa tcactggcgt acacgattta ctgattaaat tgccggtaaa
    43201 gtgcaaatta tctcctgcta ttgtggttcc actgcccagc aacatcaggc tactaataaa
    43261 aaacaacgta cgctttttct tcattggtaa tctacctgta acgtggccca ggcctcaaag
    43321 tccgcctctg gcagaacgac accactcttt tttactggca cagccgttaa caccggtaaa
    43381 tcggtttcat tcaccataag tggcgtattg atcgtaaaac tctgccctgc ctgtttgagc
    43441 tgaatgccta aaccagtgat attgctgctt actgcagcat tatcaaaatc gcttagtgtg
    43501 ccactccagc tcaaggtcat ttccattaat gggtcgcgaa ccgccgtatc gcaggtaatg
    43561 gaatacggca cggtttgctc ataattgctg ccattaatgt catcaataag cacatcctga
    43621 aaagttattt caatggtttg agcgttgcta atcgtgcagt taggcggctg cactaaataa
    43681 ccgtgaaaag agacgtttga gagtgccatc gagcaaaagc cccacagcaa aataagcgag
    43741 atccgtttca ttggtaatcc accacaattg tggcactggc gttaaattca gctgccgcta
    43801 acgttgtacc actctgtttc accggcacag cttccagaac gggaactccc gaggaaaaat
    43861 taaatggcaa ccatgagcca gaggttaagt cgagtattgt gtgatcacta cttttctgaa
    43921 cccgaatacc aaacccagga atacccgtgc tgataactgt ttcaccgtta atcacgaccg
    43981 ttgtcgcctg catctgtaga cgcaggtcat ccattgccag attgttacaa acaagcgagt
    44041 agtcaatggg acgtttataa tcaaccccat tgatcttatt aatgaataca ttaccaaact
    44101 caactgccgc accatttacc gtacaaggag gaggggcatc gacaagcacc cgtaaattca
    44161 tacttactga cttactcgct gcaactgcaa agccgctacc aacacaacat atcagtaacc
    44221 cagttaccgc ccagacagaa caagatcctt tcatactggc tctcccggcc tcagcctttg
    44281 cggctctgct cttcatcgac ctggcaactg ttaccgttgc aagtgaaaaa gagcggcatc
    44341 cgagcgccat agtcgttgac ataggtcagt accggcacgc tacccatctt cacattcagc
    44401 ggtgcagagg ttttcggcga catcactatt ggcgagaaac cggctgcagg attgccattt
    44461 ttctgtgtgc tggcattact gaaaatgacg taatacggcg tcgggttatt gacggtaaat
    44521 tcctgaccgt tgcgggttaa agtaacctta tattgccagg ggtttttcat gctgacgttc
    44581 tccagcgcct tcggtcgcca gaacaactta atacgcgttt gtagtgctat ttgcagcgtg
    44641 ttggctttgt ttgattttgg cgggatctca cgcacgttga agtagaacag gctttcacga
    44701 tcggcaggca atttgttgat atccggcatt ccctgcactt ttacctgccc gttcatcatt
    44761 gaatcgatgc gttgcaccgg tggcagaaca gttaacggtg aactgatctt gttacccttt
    44821 tcatcttcga tccagctttg ggccagataa ggcagtttcg gatcgttatt gcgcaacgtc
    44881 acgctgattg atttgtcgct ttcattaaat atcaaacggg tacgatctgg cgttacagaa
    44941 gcctgggcga caaactagca gcgctaagca gtaacgctag cgtcgtagca cccaatttgg
    45001 cagagcaaag taaatcagac atggatcatt ccttaatcac gttattggtt agctgaaaca
    45061 gaaacagggg gattggtggg tgtcacctgt tgcgtctgct cctgaatgac aggctttatg
    45121 tcatcaggaa caaccggcgc gacgttgcct ttatgctggc atggcagtaa caagccgtta
    45181 aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac accactccag
    45241 aacacactca tgtgttcgcc aggtttcacc ccagccagat aaacattgcc gtcatcatcg
    45301 acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg aggatggctg
    45361 ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc aaatttgcgg
    45421 tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc ttcggcgttt
    45481 tccggcagtt tgttgaggtc gatatacgcc tgattgcggt aatagttatt gacgtcagaa
    45541 acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc taccggaaca
    45601 tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg ggtacggtga
    45661 agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc cgcagaggtg
    45721 tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc atcatggctg
    45781 taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg atagtgagtc
    45841 gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact gccatagtta
    45901 ccgttatagc taacggtact gttgtcaccc cacggcatac tgagcgaaat gtacatgcct
    45961 ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca tcgagatgct gacattacga
    46021 atactgccca tattgaaata gtgcgagagc atgatgttgt agtttgtctg ctcctcgcga
    46081 tcccagtagg tatggcgggt atagttgaga taaaccgaaa cacccgcatc gcggaagttc
    46141 tggttataag tggcagtgta catctctttg tcgttgcccg tgcgtaccat tccgctgtca
    46201 ctggcatcca gatactcgct catggtcata aagttctctt ccgagaagcg atatccagca
    46261 aaagtaacgc ggctgttgag ctggtcaaag tctttggaat aactcacacg gaaggagtta
    46321 ccgtccagcg aacctttgcc ataagcggtg tctttatcca gtttggtgtg cgagtgagta
    46381 acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac cgacgccaag cgccgcagac
    46441 tgatagtttt catctcccag tgcgccgcca tataacgacc agccgttagc gatcccccag
    46501 gaagcttcag caccagaaaa aaattcaccc tcgacatggt gtccccactc ttgcggacgg
    46561 cccatcatga tcttatagcg aacctgacct ggacgagtga ggtatggcat cgaggcggtg
    46621 ctgatgtcat attcctgcac ctggccgttc tgttcttcaa tgcgaatatg caacgtaccg
    46681 gagacagaat caccaagatc ctgaatacga aacggtccag ccggaacctg cgtttcgtaa
    46741 atcacacgcc ccatctggct gacggtcact tttgctgtgg tgtgtgccac gccggaaatg
    46801 tctggcgcgt agccgcgcag attgggaggc aacatttgat cgtcagtact gacactgcca
    46861 ccaacatagt taaaaccatc aaaaatatcg gatctgaggt aatcctcgcc cagcgccagt
    46921 ttggctttta atgatggtaa cgcccgctag gcatagtagc gactccactc ccattttttt
    46981 tgagtttcat cgccgctgaa ttcatcgtca tcattactgc gagtatgttg atagttagtc
    47041 tgccagtcag cacgcatacg ccacggcccc aggttaaccc cgaccgtccc gttgccgctg
    47101 atctcgttac tatcatcacc gccattttct tcgtgccggg tttgtgcgtt gatgctgtag
    47161 tccgcgacga tcccggagat gccgtcatcc caacgtgaag gcggatccca gtcgggataa
    47221 gtatattcga ggtaagcctg tggcagtgaa atgactaatg cggactggct taaatcagcc
    47281 ttaatttcca tgccttccag ttgaccggat ttcaggcatt tagcatcgtg gctccattgc
    47341 agatttttcg ccacgtcttc ttttaaacca aactgcgcta ccagttccgg tgtcagacaa
    47401 gcatagcttt tgctcgcgtc atcttcacca gcataccagt aaatatcgta ctcttccgcc
    47461 aatggctgtt tatttagttg aacctgtaaa ttgtattttc cgggctcaac atatccctgg
    47521 ctggaaaaac gcttcagatc aatttttgtg tcgcctttta attccagaaa acgggaatca
    47581 aactgaatgt cgtcttcagc ccagacactg ctataactcc ctgacattgc cagcgcaatg
    47641 caccacggaa gaatccgtaa tcgaaaaaga gaatggtcag gcatacacgt ttctatccgt
    47701 ttttattctt gttcggtcaa aatccattta aatcccccat agcagtgtcg ctatgggggt
    47761 atgccttaca ggtaagtaat ctggaaggtg gtatttgctt cgaaattacc cagatctggc
    47821 gcatcagctg cgccgaccag ccaggcttta aagttcagtg tctgcttcgc tttgccttta
    47881 ttggtagaag tgtctttcac tattttctgg tcaataccca tgccgctttt gtaagaggta
    47941 cccagagagt caccgatcgc caggctaaca ttgttaaatg ccgcacaggt atcggtattg
    48001 aaaatggtgt aataattgcc gctatttgca gaagaaacgg taccagtaaa ggtagtggtc
    48061 attgtttcct gagtatcaaa tacgcaatct tgcagacgaa tctggaaact tttcggcgtg
    48121 gtggtgccgt tatttttcag tttttcggca ccgatatcac ccatatcaac ttccagtttg
    48181 tgatcgtccg gaacgataga acacactgag tcttcaatgg tgccgtaaaa acgtgcagta
    48241 ccgttgttac ccgcagcgat tgttgccgta gaggtgaaca cgcccatcac cattgccgca
    48301 gcaatagctg ttttaacaaa cttactcatt atttgtcctt aaactataaa taattaaaaa
    48361 tataaacatt gcaatacatt gattcagtca ataaccaatg tttataccca gccattgaat
    48421 acggcatgcg atccaaaaag aacaggtcgt agaaaattgt attcgttggt gtggggccgc
    48481 atagtgat
//
LOCUS       DAZOYS010000007        47767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOYS010000007 DAZOYS010000000
VERSION     DAZOYS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.007"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            59..691
                     /gene="comR"
                     /locus_tag="ACHUN9_000317"
     CDS             59..691
                     /gene="comR"
                     /locus_tag="ACHUN9_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0693833.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(753..1292)
                     /locus_tag="ACHUN9_000318"
     CDS             complement(753..1292)
                     /locus_tag="ACHUN9_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0693834.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1519..2823)
                     /gene="ndh"
                     /locus_tag="ACHUN9_000319"
     CDS             complement(1519..2823)
                     /gene="ndh"
                     /locus_tag="ACHUN9_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0693835.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHUN9_000320"
     CDS             complement(3231..3773)
                     /gene="ycfP"
                     /locus_tag="ACHUN9_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0693836.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHUN9_000321"
     CDS             complement(3796..4821)
                     /gene="nagZ"
                     /locus_tag="ACHUN9_000321"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0693837.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHUN9_000322"
     CDS             complement(4832..5656)
                     /gene="thiK"
                     /locus_tag="ACHUN9_000322"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0693838.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHUN9_000323"
     CDS             complement(5637..6278)
                     /gene="lpoB"
                     /locus_tag="ACHUN9_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0693839.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6292..6669)
                     /locus_tag="ACHUN9_000324"
     CDS             complement(6292..6669)
                     /locus_tag="ACHUN9_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0693840.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHUN9_000325"
     CDS             complement(6672..7031)
                     /gene="hinT"
                     /locus_tag="ACHUN9_000325"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0693841.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHUN9_000326"
     CDS             7365..9554
                     /gene="fhuE"
                     /locus_tag="ACHUN9_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0693842.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHUN9_000327"
     CDS             complement(9614..11047)
                     /gene="ptsG"
                     /locus_tag="ACHUN9_000327"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0693843.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11342..12139)
                     /locus_tag="ACHUN9_000328"
     CDS             complement(11342..12139)
                     /locus_tag="ACHUN9_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0693844.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHUN9_000329"
     CDS             complement(12150..13154)
                     /gene="holB"
                     /locus_tag="ACHUN9_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0693845.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHUN9_000330"
     CDS             complement(13151..13792)
                     /gene="tmk"
                     /locus_tag="ACHUN9_000330"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0693846.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHUN9_000331"
     CDS             complement(13782..14804)
                     /gene="yceG"
                     /locus_tag="ACHUN9_000331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0693847.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHUN9_000332"
     CDS             complement(14807..15616)
                     /gene="pabC"
                     /locus_tag="ACHUN9_000332"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0693848.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHUN9_000333"
     CDS             complement(15736..16977)
                     /gene="fabF"
                     /locus_tag="ACHUN9_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0693849.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHUN9_000334"
     CDS             complement(17065..17301)
                     /gene="acpP"
                     /locus_tag="ACHUN9_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0693850.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHUN9_000335"
     CDS             complement(17512..18246)
                     /gene="fabG"
                     /locus_tag="ACHUN9_000335"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0693851.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHUN9_000336"
     CDS             complement(18259..19188)
                     /gene="fabD"
                     /locus_tag="ACHUN9_000336"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0693852.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHUN9_000337"
     CDS             complement(19204..20157)
                     /gene="fabH"
                     /locus_tag="ACHUN9_000337"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0693853.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHUN9_000338"
     CDS             complement(20225..21295)
                     /gene="plsX"
                     /locus_tag="ACHUN9_000338"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0693854.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHUN9_000339"
     CDS             complement(21376..21549)
                     /gene="rpmF"
                     /locus_tag="ACHUN9_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0693855.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHUN9_000340"
     CDS             complement(21601..22122)
                     /gene="yceD"
                     /locus_tag="ACHUN9_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0693856.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22321..22905
                     /gene="yceF"
                     /locus_tag="ACHUN9_000341"
     CDS             22321..22905
                     /gene="yceF"
                     /locus_tag="ACHUN9_000341"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0693857.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHUN9_000342"
     CDS             complement(23017..23976)
                     /gene="rluC"
                     /locus_tag="ACHUN9_000342"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0693858.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24549..27734
                     /gene="rne"
                     /locus_tag="ACHUN9_000343"
     CDS             24549..27734
                     /gene="rne"
                     /locus_tag="ACHUN9_000343"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0693859.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27929..28882)
                     /gene="flgL"
                     /locus_tag="ACHUN9_000344"
     CDS             complement(27929..28882)
                     /gene="flgL"
                     /locus_tag="ACHUN9_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0693860.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28894..30537)
                     /gene="flgK"
                     /locus_tag="ACHUN9_000345"
     CDS             complement(28894..30537)
                     /gene="flgK"
                     /locus_tag="ACHUN9_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0693861.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30603..31544)
                     /gene="flgJ"
                     /locus_tag="ACHUN9_000346"
     CDS             complement(30603..31544)
                     /gene="flgJ"
                     /locus_tag="ACHUN9_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0693862.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31544..32641)
                     /gene="flgI"
                     /locus_tag="ACHUN9_000347"
     CDS             complement(31544..32641)
                     /gene="flgI"
                     /locus_tag="ACHUN9_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0693863.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32653..33351)
                     /gene="flgH"
                     /locus_tag="ACHUN9_000348"
     CDS             complement(32653..33351)
                     /gene="flgH"
                     /locus_tag="ACHUN9_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0693864.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33404..34176)
                     /gene="flgG"
                     /locus_tag="ACHUN9_000349"
                     /pseudo
     CDS             complement(33404..34176)
                     /gene="flgG"
                     /locus_tag="ACHUN9_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34348..35103)
                     /gene="flgF"
                     /locus_tag="ACHUN9_000350"
     CDS             complement(34348..35103)
                     /gene="flgF"
                     /locus_tag="ACHUN9_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0693865.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35123..36328)
                     /gene="flgE"
                     /locus_tag="ACHUN9_000351"
     CDS             complement(35123..36328)
                     /gene="flgE"
                     /locus_tag="ACHUN9_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0693866.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36353..37048)
                     /gene="flgD"
                     /locus_tag="ACHUN9_000352"
     CDS             complement(36353..37048)
                     /gene="flgD"
                     /locus_tag="ACHUN9_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0693867.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37060..37464)
                     /gene="flgC"
                     /locus_tag="ACHUN9_000353"
     CDS             complement(37060..37464)
                     /gene="flgC"
                     /locus_tag="ACHUN9_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0693868.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(37468..37884)
                     /gene="flgB"
                     /locus_tag="ACHUN9_000354"
     CDS             complement(37468..37884)
                     /gene="flgB"
                     /locus_tag="ACHUN9_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0693869.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38039..38698
                     /gene="flgA"
                     /locus_tag="ACHUN9_000355"
     CDS             38039..38698
                     /gene="flgA"
                     /locus_tag="ACHUN9_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0693870.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38774..39067
                     /gene="flgM"
                     /locus_tag="ACHUN9_000356"
     CDS             38774..39067
                     /gene="flgM"
                     /locus_tag="ACHUN9_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0693871.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39072..39488
                     /gene="flgN"
                     /locus_tag="ACHUN9_000357"
     CDS             39072..39488
                     /gene="flgN"
                     /locus_tag="ACHUN9_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0693872.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39528..41063)
                     /gene="murJ"
                     /locus_tag="ACHUN9_000358"
     CDS             complement(39528..41063)
                     /gene="murJ"
                     /locus_tag="ACHUN9_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0693873.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41173..42096)
                     /locus_tag="ACHUN9_000359"
     CDS             complement(41173..42096)
                     /locus_tag="ACHUN9_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0693874.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42098..42745)
                     /locus_tag="ACHUN9_000360"
     CDS             complement(42098..42745)
                     /locus_tag="ACHUN9_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0693875.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42756..43340)
                     /gene="rimJ"
                     /locus_tag="ACHUN9_000361"
     CDS             complement(42756..43340)
                     /gene="rimJ"
                     /locus_tag="ACHUN9_000361"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0693876.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43576..44784
                     /gene="mdtH"
                     /locus_tag="ACHUN9_000362"
     CDS             43576..44784
                     /gene="mdtH"
                     /locus_tag="ACHUN9_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0693877.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44848..45495
                     /gene="grxB"
                     /locus_tag="ACHUN9_000363"
     CDS             44848..45495
                     /gene="grxB"
                     /locus_tag="ACHUN9_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0693878.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45629..46189
                     /locus_tag="ACHUN9_000364"
     CDS             45629..46189
                     /locus_tag="ACHUN9_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0693879.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46295..47341
                     /gene="pyrC"
                     /locus_tag="ACHUN9_000365"
     CDS             46295..47341
                     /gene="pyrC"
                     /locus_tag="ACHUN9_000365"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0693880.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47415..47660
                     /gene="dinI"
                     /locus_tag="ACHUN9_000366"
     CDS             47415..47660
                     /gene="dinI"
                     /locus_tag="ACHUN9_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0693881.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11565 a        12914 c        11865 g        11423 t
ORIGIN      
        1 cggaggtgat tatttttata ataacctttc attcacagtc accaggttca acggcatcat
       61 ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag
      121 ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc
      181 tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac
      241 caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa
      301 gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt
      361 tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa
      421 caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca
      481 tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat
      541 cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat
      601 gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac
      661 tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg
      721 aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc
      781 ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc
      841 tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc
      901 gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc
      961 tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc tttaccacga
     1021 ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta
     1081 atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga
     1141 cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg
     1201 ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca
     1261 atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc
     1321 caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg
     1381 gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga cgcttaacgc
     1441 gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca
     1501 tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact
     1561 ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg
     1621 gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt
     1681 caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca gcgaaccatg
     1741 atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa tattgttcat
     1801 tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg
     1861 acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag
     1921 cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga tatctttcag
     1981 gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcacc
     2041 atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg
     2101 aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg
     2161 caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt
     2221 gtaaccgtag ctgtgcagtt gcttgaccgc attgtgcaat tcagcggaga gttctacacc
     2281 cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca ggttggcgga
     2341 gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc
     2401 gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg tgctacccag
     2461 cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc
     2521 atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc
     2581 cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc
     2641 ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat ggctgtggtt
     2701 acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt
     2761 tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt
     2821 caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa acatagctct
     2881 taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg
     2941 tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact
     3001 ttaccgggtg agatcaaaaa acagagttta ttgtttatta tttgagcaag tccggaaggg
     3061 tgatataggg tgacgcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc
     3121 gcatcaggca ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc gacttacaga
     3181 attcggagcg catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg
     3241 gttttgaacg ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc
     3301 tcttcgtccc agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta
     3361 agcgcttcat cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcgcggaag
     3421 ttggtcacac acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc
     3481 atgttctcat aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa
     3541 ccaatccgtt ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg
     3601 acgttcagtt gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga
     3661 tgccgcgtac tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact
     3721 ttctcgtggt tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc
     3781 tcacataagc cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt
     3841 cagacgggtg ctgatcgcct tccagcgagc cgagtccatc agttcctgtc gcgaaaatga
     3901 acctttatga tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac
     3961 gctgacggcc cctttacgat tattgcagac caggatcata tcgcaacctg cgtccagtga
     4021 tgcctgcccg cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc
     4081 agagaaaatt acaccgtcaa aacacagttc ctgacgcaaa acggttttca gccagtagga
     4141 agaaccgctc gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc
     4201 gtcgagttta ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc
     4261 cgcttgtgga cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg
     4321 tcctgggaag tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg
     4381 gctggcaatt gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc
     4441 gctgatatgc ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat
     4501 ttcgctggcc atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa
     4561 cagcgcagca aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac
     4621 gcgtccacct tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg
     4681 gcgcaccagt tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc
     4741 tcccaccagc ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac
     4801 atccaacatt actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg
     4861 tgtcatcggc cagcctgata aattgttgat cgccgatttg tcgccagcgg tactcaaacc
     4921 accctgcttt gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg
     4981 ccggataaat cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat
     5041 tttccaccca caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt
     5101 cgatgagttt taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca
     5161 atggacttaa gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca
     5221 gccaacccac agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca
     5281 acagcgttat tcgccagcca aaacgtggtt gttgatgaag ataatacagc aagcctgcca
     5341 gttcgttggt atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc
     5401 agtcacggag atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc
     5461 gatactggcg caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa
     5521 aacgctggcc ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg
     5581 tgacgggatg aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat
     5641 tgctgcgaaa cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc
     5701 tgcatttgta gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga
     5761 gcgccgacat tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc
     5821 gacaaaccta actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc
     5881 ccgttattag ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg
     5941 ttagtacggt tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc
     6001 ccaagcatct tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc
     6061 ggcgcaggcg gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc
     6121 ggcaccgagg gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt
     6181 ttcacttctt ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc
     6241 agcgcggtaa tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata
     6301 tagataaagt ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc
     6361 cgaatgtgcg ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct
     6421 ggcgtcatac cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag
     6481 cgttgaggat gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat
     6541 gcccgcagcc aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc
     6601 cggatgtgaa cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc
     6661 ttttctcatc gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac
     6721 aagtgcatat ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga
     6781 cgatagccat cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg
     6841 cgtcccagcg cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc
     6901 ggaatgatca gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga
     6961 tcatcctggt agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt
     7021 tcttctgcca cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg
     7081 ccacactgcg agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga
     7141 tttcttaagc aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct
     7201 gcgaatgcgc tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgcatat
     7261 ttctcatttg catttacaaa caaaattatt cgcacactaa atataaccgc tcatcaccct
     7321 cctccggcat gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat
     7381 ttaacaggga taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag
     7441 cactggcact attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg
     7501 ttgagggttc agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct
     7561 ctgcgggtac caaaatgcag atgactcaac ttgatattcc tcagtcggtc actattgtta
     7621 gccagcagcg gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc
     7681 tggggatcag caaaagtcag gcggattccg atcgtgctct ttattattcc cgtggattcc
     7741 agatcgataa ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg
     7801 gcgacgcact ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag
     7861 gactcatgac cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga
     7921 ccagtcgtga atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt
     7981 atgtggcgga tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg
     8041 gcggctacca gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct
     8101 cgggcattgt cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc
     8161 agcgcattga tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca
     8221 gcagcaacag ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac aacgataaag
     8281 agatcaacaa ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac
     8341 tgaatgccac ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg
     8401 taaacaaagc ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt
     8461 atgtcggcgg caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg
     8521 ctgacggtag ttatgaattg tttggtcgtc agcacaatct agtgtttggt ggcagttaca
     8581 gcaaacaaaa caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca
     8641 gtttctacaa ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcac
     8701 aggacgatac cacacatatg aaatcgttat atgctgctac tcgagtcacc cttgccgatc
     8761 cgctgcatct gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca
     8821 gcatggagaa aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact
     8881 ggtcgaccta cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag
     8941 gcaaatatct ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga
     9001 tgaatagccg tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc
     9061 agtccaccgg tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg
     9121 ggacagtcag taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc
     9181 tgacatttgg cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta
     9241 atctgccacg caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt
     9301 tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt
     9361 atggcacctt ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc
     9421 aggtgacgaa aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg
     9481 ataccaacat ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca
     9541 cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat ctggcggcct tagtctcccc
     9601 aacgtcttac ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg
     9661 atttagtacc gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca
     9721 gtttcttcag gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg
     9781 taatacatgc gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg
     9841 gtgccatttc gctggtacct gttgcttttg catcttcagt cgcgtcttca cgacccggcg
     9901 ttttcagatc cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac
     9961 cgataccgac gatcgggaac agccacagtt tgctgctgtt accagacaga acgataaagt
    10021 cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga
    10081 atgccaggcc tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg
    10141 agaactcgat cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa
    10201 taccgcccac tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag
    10261 ctggcagacc gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac
    10321 ccgccatata acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac
    10381 caatctgcat ctggaaaggt acgttccaga tgtggtgcag accaaacggc accaggcaac
    10441 gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag
    10501 agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa
    10561 agatggcagc caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat
    10621 actcaggcag cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga
    10681 taatccctcc gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta
    10741 aatgcagtac cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa
    10801 ctgcggccag cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa
    10861 aaatcagtgg catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa
    10921 cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca
    10981 gtacggatac cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct
    11041 taaacataat tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca
    11101 aggggggaga gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt
    11161 aaaataattc agtgccgatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag
    11221 aagttgcaca ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc
    11281 aattttcggc ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca
    11341 ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg
    11401 tggttacctg cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa
    11461 cgtcacgaac catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag
    11521 gtgagtcagt ttccaccagt aaccgatcca gggggacata acgcgcagca tctcgaagtt
    11581 gctccgcatt acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta
    11641 atttacccgc cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca
    11701 ctttttcttc gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa
    11761 ccggcttgtt cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgaa
    11821 ctttagtttc cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct
    11881 cttctgccgc cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat
    11941 gcacgccaca agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat
    12001 aacccggtaa tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg
    12061 ccagaacgtc atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt
    12121 gtgagtcgac taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc
    12181 ggttgcaggt aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg
    12241 cctgtgacag acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg
    12301 cgcgagggag aaagatggtt tgccagttcg acgacaaggc ccggcacatc aacattggtc
    12361 acctgcgcag caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt
    12421 aaacgcgccg gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat
    12481 ggcacgctat atgccaatgc ctgacacaat gtttcacgag cctgccagtt atctccctga
    12541 aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca
    12601 tcctgtgaca ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc
    12661 gcaaggtaat gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg
    12721 gtagccagga aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta
    12781 gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg
    12841 cgtgcgtgct cattcagctt ttcggtgacc tcacgtactg catcaatgcc cagcgtattt
    12901 ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga
    12961 catccacgac agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg
    13021 ctcagggcgt agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca
    13081 tggtgacctc ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat
    13141 ggataccatc tcatgcgtcc aactccttca cccagtgggt cacggtagtg cggattgcat
    13201 ccatcacggc ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag
    13261 ccagttccag atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac
    13321 gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat
    13381 agagcgttaa gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat
    13441 gttggtcaat accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc
    13501 caatcaccca ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc
    13561 gcgcggcata aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt
    13621 tgatatccag caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag
    13681 tgaaaaccat gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg
    13741 tagttttgcc tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc
    13801 ttaagcactt tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac
    13861 gtgtgaccac ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca
    13921 gcagccttca gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg
    13981 gtataggtgt tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa
    14041 cgctctccca tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa
    14101 cggttgataa ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg
    14161 ataattgatg ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc
    14221 ccttcccagg cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg
    14281 acatcggtgg tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac
    14341 tccgggtttt ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg
    14401 tgcttgatat acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct
    14461 accagtcgca gcgggaactg tgcttcttta ccgctttcca gcaatttcag catctcgcgc
    14521 acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg
    14581 atacgcagca accattgaaa aacccgtggg cgattgatga tcttatcggc ataaagctgt
    14641 tcaccaagcg ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata
    14701 agcaatttgc tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc
    14761 agtaccacca gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct
    14821 cacaaagtgg ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg
    14881 cacgtacggg catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg
    14941 cctcttccag agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga
    15001 tacagaattg tcgcataatg ccgttaacac ctgcctgatc cagacgcggc gtataaacta
    15061 cattgccctt ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt
    15121 caaggaccag cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct
    15181 caagccgatt aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc
    15241 ttagcgccaa cgtcatccct tcgttacgca aacggtcgta atgtgcagga taaaccgtaa
    15301 cggagagaat ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac
    15361 cgcgactgat cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct
    15421 cttgttcaag ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa gcatcctgta
    15481 gtcgctggat atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa
    15541 aacaaccatc accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttatgac
    15601 cgttaattaa gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac
    15661 tacggaacaa gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg
    15721 gtgggaaatg acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc
    15781 gaaggagtta cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa
    15841 atccagatcg caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc
    15901 gcgcagcgcc aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc
    15961 ggtcatagat ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt
    16021 caccgcctgc gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta
    16081 gccaatctga ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg ccagagctgc
    16141 gcctgcgcca ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc
    16201 gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac
    16261 cagcataccg gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct
    16321 cgccgcttgc gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac
    16381 acccagcggc gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata
    16441 cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat
    16501 agagatgctt gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac
    16561 aatcgttgac ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt
    16621 gtggttttct tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg
    16681 ggttgcgttc tcttccgtta tatcaaggcc agaatcctgc atggcctgaa cgccagcgac
    16741 aattccatat tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc
    16801 acagttaaaa tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg
    16861 gtcgattagg ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt
    16921 attgccgaca ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt
    16981 tgtcctccag ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc
    17041 gaacgaccgc ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc
    17101 ctgaacggtg gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc
    17161 cagagccatt accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga
    17221 agcattgttg gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac
    17281 gcgttcttcg atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt
    17341 tttcgtagtg tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga
    17401 ttttacgtta ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta
    17461 ctcataacca cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat
    17521 gtacatcccg ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc
    17581 caggaatgca accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg
    17641 cgccaggata cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa
    17701 gcccggagca acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt
    17761 actgaagccg atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat
    17821 ggtaccaacc acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg
    17881 cattaccgct tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca
    17941 ctcttcatct ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag
    18001 gatatccact tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg
    18061 gtcggtcaca ttcaacatca gacctttgcc gttggcacct aaataatcgc tgatcgcctg
    18121 agcgccattt tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc
    18181 agcaattgcg cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa
    18241 attcatgatt ttcctctttt aaagctcaag cgccgctgcc atcgctgaag gttcgttcag
    18301 cgccgaggcg gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg
    18361 gccgacttca tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca
    18421 ctgaaccggg ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc
    18481 gcatttcaca tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc
    18541 taattctact gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct
    18601 cactggtaac ggcagagcac gttttgcgcc cgcagcttta caggcagcgc cagcacgctc
    18661 aaccgcttct ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga
    18721 aacgacctga cctgctgcag cttcttcaca cgctttcgca atagacgcat catccagacc
    18781 gatgattgcc gccatagcgc ccgtaccttc cggtacggct tcttgcatga gcttgccgcg
    18841 catctcaacc agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga
    18901 atattccccc aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca
    18961 tacgcggtac agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc aggttttatt
    19021 cagttcttca gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga
    19081 agcttcagca aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc
    19141 aacggtttga gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt
    19201 atcctagaaa cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag
    19261 aaccaactgc cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac
    19321 agaggccgca gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat
    19381 accgagtttt ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca
    19441 gtccagttga gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag
    19501 ttccgttacc gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc
    19561 tggattcaca cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg
    19621 cagatgggtg gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc
    19681 atcgccaaaa ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc
    19741 ggaaccgacg accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct
    19801 taatgcatag gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat
    19861 gcccagcatg ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt
    19921 tgccacaacg atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt
    19981 cgccgcttca aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg
    20041 gataccggta cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc
    20101 gtttgtccgc acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta
    20161 cgctcagtca cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt
    20221 cctgctaccg cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata
    20281 cagattccag gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct
    20341 gttcaatcgc gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc
    20401 cgcgcaatcc taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc
    20461 tcgtcaggct cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc
    20521 cttcaccctg agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta
    20581 atgtgacatt tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact
    20641 cattggcttc aagatagccg atataattga tagaagggat tgttttaagc accgctgagg
    20701 catcccgaat actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc
    20761 gaggattggg aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca
    20821 ccaacattgt actgtcacaa tcgacgttgg cccctaagtc aaggaccacc gttttgccct
    20881 tttgctgatg tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga
    20941 gtaataattt tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg
    21001 ctcgaccttc tttcaccagc tccagggcca cgcgcattga actcccacga ctggcgcgga
    21061 tagcttgcga aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac
    21121 gcgaacgttg ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga
    21181 ctaaaagaag agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca
    21241 cggaagggcc aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat
    21301 cgtcgccgcc cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat
    21361 cacgcagacg cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt
    21421 gatgtggtga cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc
    21481 ggtcagcgcg tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg
    21541 tacggccatg gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa
    21601 ttacttacgc tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg
    21661 cagttcacca aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg
    21721 aactaccggc aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc
    21781 gaattcgtta acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga
    21841 acgcacagga ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg
    21901 ctgacactcg agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt
    21961 atcgatagcg aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc
    22021 gacgcgctca acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt
    22081 acgaaccgga tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact
    22141 ttgtaacgtc atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg
    22201 cacacattgc gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca
    22261 aaacgaagcg catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat
    22321 atgcctaaac ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg
    22381 caaatctctt ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca
    22441 ccgcgacagt tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat
    22501 ccggatcatt taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt
    22561 aaaccgttaa cggaagaaaa tgcctgtctg caattacgca aagccagcgg caatatcgtc
    22621 accttctata ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg
    22681 gagccttttg acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt
    22741 aaggagcatc ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg
    22801 tttgagcgtt tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg
    22861 tgtcagatgt tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgctggat
    22921 gcggttacgt cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc
    22981 aggccaaccg cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg
    23041 caaacaacgc ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc
    23101 cggatgggta aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc
    23161 ttcagtgagc tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg
    23221 acccgcatac tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg
    23281 caccagggtg gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc
    23341 ttcctgactg acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc
    23401 ttgaacgctc ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg
    23461 cattcctttt tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctat
    23521 cagcaacaca cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc
    23581 cgggcgcaac gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac
    23641 cgccgtaccg gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc
    23701 cgccagcgcc gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac
    23761 gcgaaccggt gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg
    23821 ttttttgttc acccgcactt cgcctttacg caaaatacgg taaatcatac tttttggtac
    23881 gcctttcaat tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat
    23941 agcaacaatt tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac
    24001 aggattcgcg ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact
    24061 acgtgatcac tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg
    24121 agtaagcggg tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa
    24181 tgaggccgtt tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt
    24241 gcccgaccga tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc
    24301 gggctgcggg ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt
    24361 ctgtttcttt gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg
    24421 cctctttgag ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt
    24481 tcacacccgg aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt
    24541 aagttacgat gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc
    24601 ttgtagatgg gcagcgtctt tatgacctgg atatcgaaag tccagggcac gagcagaaaa
    24661 aggcaaacat ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg
    24721 ttgattacgg cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt
    24781 tccctgctaa ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc
    24841 aggaagtcat tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca
    24901 cctttatcag tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg
    24961 gcatttctcg ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc
    25021 ttgaactgcc ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg
    25081 aggcgctgca atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg
    25141 ctgaaagccg cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat
    25201 tccgcgatta cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg
    25261 aactggcacg tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac
    25321 tgtataccgg cgagatcccg ctgttcagcc actaccagat tgagtcgcag atcgagtccg
    25381 ccttccagcg tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac agcaccgaag
    25441 cgttaacggc catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa
    25501 ccgcgtttaa caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg
    25561 acctcggcgg cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg
    25621 cggtagaaaa ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc
    25681 atatttctcg ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg
    25741 aatccagcca tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat
    25801 cgctgtcgct ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg
    25861 aagttcacgc cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg
    25921 cggtgaatgc gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc
    25981 agatggaaac cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct
    26041 taagctacat gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt
    26101 tcgctgaacg taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc
    26161 cgccagcgcc aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg
    26221 caacggcaac accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac
    26281 tgaaagcgct gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag
    26341 cagaagcgaa accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg
    26401 accgtaatga gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag
    26461 aaaaccgtcg taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc
    26521 agcaggctga ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg
    26581 aacgtagccg ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga
    26641 atgttgaaga gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc
    26701 cgcgtcgtaa acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag
    26761 aagcggtagt cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag
    26821 ctccagctcc acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac
    26881 cagaacagca agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt
    26941 ctcgccgctc gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg
    27001 agcgttatcc aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg
    27061 cctctggcaa agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag
    27121 agcagcgcga acaggaagaa gtacaagtgc agccgatggt gactggggtc cctgtcgccg
    27181 ccgctgtcga acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa gttgtagaag
    27241 cccccgttca gattgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga
    27301 ttgccgccgc agtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc
    27361 aggaagttgc agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg
    27421 aagaagttgc cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg
    27481 ccgcgccagt cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg gttgaacctg
    27541 aggtcaccgt tgagcataac cacgctacgg tgccgatgac gcgcgctccg gcaccggaat
    27601 atgttccgga tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta
    27661 aaggtgccgc aggtggtcat acggcaacac atcatgcctc tgccgctcct gcgcgtccgc
    27721 aacctgttga gtaataatta gctcaagtaa tcaagccctg gtaactgcca gggctttttt
    27781 atttcatctt tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat
    27841 gcaaaatagg caaaaaaatg cccgccccgg cgatcccaga gcaggcagac aaaacatacc
    27901 cagtttcatg atatgtttta aagcgaaatt atttgttgag ctggaagagc gacaatccct
    27961 gcatatcggt aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg
    28021 aaatagttgc attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag
    28081 cgcgatcgct acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg
    28141 cgcgaacggt cagcacattg ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg
    28201 ccgcagcggc ggtttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga
    28261 tggcgctatc cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta
    28321 ccgcgttact ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac
    28381 gcgaagcatc gacctgttgc ttaatacttt ctgttccgcc gacataatca ccgttagcct
    28441 cactaaacgg cgcagtctct gttttataac cggcaaaaat gtagcgccca ttaccgtcag
    28501 tggtgttcgc cagattcagc aactggtcac gaagcccctg aatatccgta gccagcgagg
    28561 cccggtcatc gtcactcaag gtgccattgc tggcgtagac aattttttcc tgagcattct
    28621 ggatagcagt ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga
    28681 aagtacgcgc cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt
    28741 gtgatgcagc aatgggatca tctgaagggt taacgactcg cttacccgtc gacatctgtt
    28801 cgccgtactt catccattct gcctgagaat tggtgatacc acgcatgttt tgctggtaca
    28861 tcatctgggt actgaaacgc attttatctc cccttagcga atgttaatca gcgcatcaaa
    28921 aatcgcgttt gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg
    28981 cagatttccg tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag
    29041 ctgcgtcacc acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc
    29101 gatatcactc actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt
    29161 actgttgctt tgcagatcca gcagggcctg accgttgcgg ttatcgctat cacccgcatc
    29221 tttttcgctc gccatcgcta ttttcgcttc gtcggtgatt aatacatcca tgttgacgat
    29281 ggcgtcactt actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt
    29341 caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt
    29401 attgctggca aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt
    29461 cgccagtacc acggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt
    29521 cgtgttttgc agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag catcgccgtt
    29581 agcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg
    29641 tccaagcgta ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa tgccgcccag
    29701 cgacccggta ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacata
    29761 agcgacagtc gtacgagaag ggtcagcgct ggaaggaact gccgccagtt gccgcgccgt
    29821 acttccctga accagtgagt aaccattggc caccgtgatg ttataagtgc cgccatcctg
    29881 aacgctgact tctacaccaa caatctggtt taattcgctc accagttgat cgcgttgatc
    29941 cagcagattg ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt
    30001 caggctggca atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat
    30061 attgacctgt ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa
    30121 cccttctgat ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag
    30181 cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga
    30241 gagcatattg tcgattttcg acatctgctc atagcgggcg gtcaggccgc tactttgcgt
    30301 ctgcgcagca cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac
    30361 gtagacgcca ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat
    30421 agtggtttgg cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt
    30481 taacgccgcc tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga
    30541 ggttccttat aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt
    30601 attcagaaca gattatcaat gttcatgctg taggttttgc tcaccttgtc gcttatcgat
    30661 ttcatctgct ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatacccc
    30721 gcgtcctgta gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac ggcggcgtag
    30781 cgcgggttac gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag
    30841 acccgaaatt tcgcttttac tttcttcgct tcgccatttt catattcagt cgtggtgatt
    30901 tcagtaactg gacctttcca gttgccagag gctttgacgc caaacaggtt atagctcggc
    30961 tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag actccagcgc cgcctgagcg
    31021 aggatcaaat gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt
    31081 tgcgcgagga atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc
    31141 ttttgcacca gctgcgaaag cgcctgattt tgataacgca ccacagtttc gagcgggaat
    31201 ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta
    31261 accatcatct ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatctgctgg
    31321 tcatacatac tggtatacag gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa
    31381 gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc
    31441 ggacggatat ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt gagtgattgc
    31501 gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt
    31561 ttgcccgcag acatcccgca ctttgcattg attgcagtat ggacatcaga tccatcggcg
    31621 tagcgcccag cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc
    31681 tttgcagcga accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct
    31741 gtccaccacc aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg
    31801 agagatttcc ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg
    31861 aaccggtgcg cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat
    31921 tctggatatc tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa
    31981 tagtccgcgc atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag
    32041 cgatttgctg cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga
    32101 cgccaaactg gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt
    32161 tcagttggtt cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa
    32221 tattgccctg cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca
    32281 acgtacctcc acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt
    32341 gcccctgacg tccaaacgga ggaagtgact ctgtcaccat taccgcagcg acgtttttta
    32401 gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg
    32461 tttgtgtggt aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca
    32521 gaccatagcc aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac
    32581 gctcagcctg agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca
    32641 ccacagcctc acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt
    32701 ttgcgcttcg ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt
    32761 agacggtacg gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat
    32821 aaattcggta ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt
    32881 gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc
    32941 cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc
    33001 aaacagcccc tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg
    33061 gctggcattc gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag
    33121 cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat
    33181 cggctgagca gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg
    33241 tgcactggtc gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga
    33301 aagcaccaac aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat
    33361 cttcaaaaat cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt
    33421 tttgcagcat ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca
    33481 cctgaatcat attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt
    33541 gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca ccagaggatt
    33601 gcgtttcggt gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg
    33661 tgagattgag ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca
    33721 cgccatcacg accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca
    33781 cctgaaaacc accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc
    33841 tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt
    33901 gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg
    33961 tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc
    34021 cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc
    34081 gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc
    34141 aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg
    34201 acaaagtgca tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg cctacaagtg
    34261 ctcgcaaaat cgataaattg cgattgcccc catagacccg ataaacttgc gcatcgggca
    34321 acttatactt gtcatgtatc ctttcaatta actcatcgac agcagttggt tggcacggcc
    34381 tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc
    34441 aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat
    34501 cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct
    34561 taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt
    34621 cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt
    34681 gccatcggca gcaatagtga tttccgctcc ttccggcaca gcaattggcc ccgcctcgcc
    34741 tatcacctga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt
    34801 acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccagccat cctgctgcaa
    34861 cgcgacgtcc agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat ctgcgcccgg
    34921 cgttgacgcc gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg gcaccgcgcg
    34981 taaagcattc aactgcgcgc gaaaaccggg cgttgaggca ttggccagat tactggcggt
    35041 taccgcctgt tgattcagtg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc
    35101 cattgagcta tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct
    35161 gagttttgat ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca
    35221 gttctttact gagatcgaca ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt
    35281 tgcccgtccc ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt
    35341 tgtcgccttc ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca
    35401 gcagttgtgt ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat
    35461 aactcaccag atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc
    35521 cggtattttg ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt
    35581 taattgcgcc agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa
    35641 acaccagctt cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga
    35701 cctgccagtt attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct
    35761 gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga
    35821 atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg
    35881 tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt
    35941 gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat
    36001 tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac
    36061 cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc
    36121 cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca
    36181 gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt
    36241 aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta
    36301 atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta
    36361 tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc
    36421 tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg
    36481 taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat
    36541 cagtcaacgt accgtcccag gtgaaactgt ggactccggc ggtcagttca ccaatatcaa
    36601 tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct
    36661 gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc
    36721 ccgcaagaac agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta
    36781 acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa
    36841 tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat
    36901 tggtcgggtc ctggtttttc agctgcgcca ccaacaaagt cagaaaactg ctttgtaagt
    36961 ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg
    37021 gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt
    37081 tttcagcatc atgcttttca ccatgttgag cacttcaaca ttggcctgat agctgcgtga
    37141 cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc
    37201 ctttgcatcc gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc
    37261 tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa
    37321 taccacctgt tttgcccgat atggctgtcc atcgggaccg gtcacgctat cagcattcgc
    37381 cagattactg gccgccacgt tcaagcgctg ggactgggca gttaacgccg acccggcgat
    37441 atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc
    37501 tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa
    37561 ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat
    37621 acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccggaatgt gttgcgttga
    37681 ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag
    37741 ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt
    37801 tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa
    37861 ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct
    37921 taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc
    37981 cgttgatgac cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat
    38041 gctggcaata aaacgtagcg tggcgatcat ctcgatacta ttcactccgt taagtgcggc
    38101 gagtaatctc acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg gggtaagtga
    38161 tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccgtgcg agcagccatt
    38221 gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg
    38281 taacgacaaa cgatatttac aggttaatgt acaggccaca ggaaattatg tggttgccgc
    38341 gatgcccatt gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg
    38401 gcaggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag
    38461 cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg
    38521 ggtaaaagcg ggacaacgcg tcaacgtgat cgccagcggt aacgggttta gcgccaacgc
    38581 agaaggtcag gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc gcatggtatc
    38641 gggactggtg gtcagcggcg ttgttgatgc agatgggaat attcttataa accagtaagc
    38701 tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa
    38761 tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcccgtaa gcaccgttca
    38821 accacgcgaa accactgacg cgtcggtaac gaacacccgg gcggcaaaaa caacagcctc
    38881 caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag
    38941 tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa
    39001 aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag
    39061 taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg
    39121 atctcaaaac ggtaatggat caagaacagc aacatctctc tatggggcag atcaacggca
    39181 gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg
    39241 aacagttacg caggaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc
    39301 aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac
    39361 tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac
    39421 cgacgctata tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt
    39481 cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg
    39541 cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc
    39601 cccgccagca cgaccgccat taaacgcagt aagcgccaag gcatggtacc caatgaccac
    39661 tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc
    39721 agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac
    39781 tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg
    39841 gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg
    39901 atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact
    39961 ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc
    40021 agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacccgaa
    40081 agaatgccca acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag
    40141 tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc
    40201 agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg
    40261 gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg
    40321 attaaggaga tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg
    40381 cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc
    40441 aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc
    40501 gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg
    40561 ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct
    40621 cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg
    40681 ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc
    40741 cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca
    40801 gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc
    40861 agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta
    40921 ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg
    40981 acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg
    41041 gccagcgatt ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc
    41101 aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag
    41161 atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc
    41221 cagcactgcc tgttcgccgg cggtttgcag aactgtctga ttttgcacac attcaatgaa
    41281 gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg
    41341 tttatgcact acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat
    41401 gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat
    41461 acaggtggtg atctgcaaag gatcggccga aaaatggtgc tcggcaaaca gcatttcgcc
    41521 agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag
    41581 cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc
    41641 gacactattg ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt
    41701 taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt
    41761 ccgcgccgcc agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc
    41821 gacacagaca tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt
    41881 gctggaatgc acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata
    41941 aggaatgcgc cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc
    42001 ttgtaacatc cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc
    42061 gcctaatccc acaacgccga tacgtaattt tttcacttta atctcccaga tgggccagca
    42121 acgaatcaag acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct
    42181 gtaaatcacc atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc tctacctcac
    42241 cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca
    42301 caaaaggacc atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat
    42361 cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac
    42421 gcaataacaa cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt
    42481 ctgaattaca aaaacgttgt tcatatttgg tgacccgatt accaaagcca ctcaccgtgc
    42541 gcagataatg gcgtttgacc agattatcca gttgctcctg cacttcagat tcactcagat
    42601 tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga
    42661 gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt
    42721 caagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg
    42781 gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc
    42841 gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc
    42901 ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct
    42961 gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg
    43021 agatagcagg catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc
    43081 tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg
    43141 ttaatcatcc ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact
    43201 ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca
    43261 tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg
    43321 ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt
    43381 aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca
    43441 ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt
    43501 ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat
    43561 ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg
    43621 ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc
    43681 cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc
    43741 cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc
    43801 aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc
    43861 gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg
    43921 tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgttgtcgt
    43981 tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg
    44041 agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg
    44101 ctgtgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg
    44161 cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg
    44221 acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc
    44281 aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg
    44341 tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac
    44401 cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc
    44461 ggtctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag
    44521 cctgcgcgtg aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg cagctatatg
    44581 gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg
    44641 ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt
    44701 attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgcaacgcgt
    44761 cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt
    44821 gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac
    44881 tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg
    44941 ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg
    45001 caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa
    45061 ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc
    45121 aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat
    45181 gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt
    45241 aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg
    45301 cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat
    45361 gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca
    45421 agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca
    45481 tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt
    45541 tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat
    45601 tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat
    45661 cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga
    45721 aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt
    45781 ggctgacgcc catattgttc tgacaaactt gatcagccaa attggtcgcg aagagccgaa
    45841 taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa
    45901 agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat
    45961 cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt
    46021 gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc
    46081 tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa
    46141 aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg
    46201 cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg
    46261 caaaaacatt ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa
    46321 gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt
    46381 cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc
    46441 cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc
    46501 cgggcacgat ttcaccccat tgataacctg ttatttaaca gattcgctgg atcctaatga
    46561 gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc
    46621 aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg
    46681 catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga
    46741 catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct
    46801 gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg
    46861 tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa
    46921 ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa
    46981 tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg
    47041 tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg
    47101 cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct
    47161 gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatgggt tgccggtcaa
    47221 cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac
    47281 tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata
    47341 aaaatcgtag cttcctgttg tcattaggtt atttaacctg tataaataac cagtatattc
    47401 aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg
    47461 gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata
    47521 atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa
    47581 caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg
    47641 actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt
    47701 tttattttac ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca
    47761 atatgct
//
LOCUS       DAZOYS010000008        46735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOYS010000008 DAZOYS010000000
VERSION     DAZOYS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.008"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUN9_000367"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUN9_000367"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0693882.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHUN9_000368"
     CDS             973..1341
                     /locus_tag="ACHUN9_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0693883.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUN9_000369"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUN9_000369"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0693884.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUN9_000370"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUN9_000370"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0693885.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUN9_000371"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUN9_000371"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0693886.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUN9_000372"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUN9_000372"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0693887.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUN9_000373"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUN9_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0693888.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUN9_000374"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUN9_000374"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0693889.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUN9_000375"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUN9_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0693890.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUN9_000376"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUN9_000376"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0693891.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHUN9_000377"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHUN9_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUN9_000378"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUN9_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0693892.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUN9_000379"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUN9_000379"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0693893.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAYLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHUN9_000380"
     CDS             complement(12998..13573)
                     /locus_tag="ACHUN9_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0693894.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHUN9_000381"
     CDS             complement(13634..14083)
                     /locus_tag="ACHUN9_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0693895.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHUN9_000382"
     CDS             complement(14070..14495)
                     /locus_tag="ACHUN9_000382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0693896.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUN9_000383"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUN9_000383"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0693897.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUN9_000384"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUN9_000384"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0693898.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUN9_000385"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUN9_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0693899.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUN9_000386"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUN9_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0693900.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUN9_000387"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUN9_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0693901.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUN9_000388"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUN9_000388"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0693902.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUN9_000389"
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUN9_000389"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR0693903.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUN9_000390"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUN9_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0693904.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUN9_000391"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUN9_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0693905.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUN9_000392"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUN9_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0693906.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUN9_000393"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUN9_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0693907.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHUN9_000394"
     CDS             complement(26015..27418)
                     /locus_tag="ACHUN9_000394"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0693908.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUN9_000395"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUN9_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0693909.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUN9_000396"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUN9_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0693910.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUN9_000397"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUN9_000397"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0693911.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUN9_000398"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUN9_000398"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0693912.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUN9_000399"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUN9_000399"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0693913.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUN9_000400"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUN9_000400"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0693914.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUN9_000401"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUN9_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0693915.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUN9_000402"
     CDS             complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUN9_000402"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0693916.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34334..35284
                     /gene="tal"
                     /locus_tag="ACHUN9_000403"
     CDS             34334..35284
                     /gene="tal"
                     /locus_tag="ACHUN9_000403"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0693917.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUN9_000404"
     CDS             35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUN9_000404"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0693918.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAHEKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37403..38446)
                     /locus_tag="ACHUN9_000405"
     CDS             complement(37403..38446)
                     /locus_tag="ACHUN9_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0693919.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUN9_000406"
                     /pseudo
     CDS             complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUN9_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUN9_000407"
     CDS             complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUN9_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0693920.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUN9_000408"
     CDS             41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUN9_000408"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0693921.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUN9_000409"
     CDS             43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUN9_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0693922.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUN9_000410"
     CDS             complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUN9_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0693923.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10689 a        13151 c        12112 g        10783 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatac gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgccag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcgtca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctggtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accgggttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acgcagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt tattattcgc
    31681 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
    31741 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
    31801 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
    31861 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
    31921 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
    31981 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgcc gcatcgccgt
    32041 gcatttcacc atcaatcatc agttctggcg cacgttccct gaccagttcc agcgcctgac
    32101 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
    32161 cgcgcggttc aataccgaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
    32221 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
    32281 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
    32341 ttttcaccac gctaaaatgt tcatgataat cacccaccgt accgcaaatc attgcatcgg
    32401 cttcgccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
    32461 gcgcctgttc ctgagtgacg ccgcgacgct tcatgatctg gaagtattcg gtccagtact
    32521 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
    32581 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
    32641 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
    32701 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
    32761 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
    32821 gacgcgtcgc cacgcccgac tccatcgccg ctttcgcgac cgcaggagcg atcttaacga
    32881 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
    32941 cgccatacgc tgaagccacc acttcgctct gttccgcatg ggccagttct gcaatcgcgc
    33001 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
    33061 ggaagatgaa cgggaagcaa aggacgttgt tgacctggtt tggatagtca gaacgaccgg
    33121 tgcagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
    33181 gattcgccag cgccagaatc attggtgccc gagccatttt cttcaccatt tcctgggtca
    33241 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
    33301 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccatg tttggctcac
    33361 ggccttgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
    33421 gcgctaccag cagattcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
    33481 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
    33541 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
    33601 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
    33661 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
    33721 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
    33781 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
    33841 ccgccaccag gttaccgcgg gcggtatatt tgtaggcttt taacgggtct ttttcgattt
    33901 caagacaagg tgcggcaacg ccgggtgagt aggccagcgc cagatcgcgc tgcgttgcca
    33961 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
    34021 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
    34081 gtgtgtgggc aacaccccct gcccacaacg tggcgcacat tattaccttg ccggagtcta
    34141 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
    34201 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
    34261 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
    34321 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
    34381 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
    34441 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
    34501 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
    34561 ggcggtcaat ttcggtgctg aaatcctcaa aatcgtaccc ggtcgcgtgt caacagaagt
    34621 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
    34681 cttgtatcag caacaaggcg ttgagaaatc acgcattctg atcaagctgg cttcgacctg
    34741 ggaaggaatt cgcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct
    34801 gctgttttct tttgcacagg cacgggcctg tgcggaagca ggcgtttttc tgatttcgcc
    34861 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
    34921 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
    34981 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
    35041 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
    35101 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
    35161 cgaagcggag ttccgttggg agcacaatca gtatgcgatg gcggtagaaa aactgtctga
    35221 aggcattcgt ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
    35281 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
    35341 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
    35401 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaacct
    35461 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
    35521 tgctgcatct gaccggttac gacctgccgc tggaagagct gaagaacttt cgtcaactgc
    35581 attcgaaaac cccaggccac ccggagattg gttatacgcc aggcgttgaa accaccaccg
    35641 gtccacttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
    35701 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
    35761 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
    35821 gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
    35881 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
    35941 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
    36001 tgaaagataa gccgtcgctg attatctgtc gtacggtgat tggctttgga tcgccgaata
    36061 aagcaggtaa ggaagaggcg cacggcgcac cgctggggga agaagaagtg gcgctggcac
    36121 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagatatt tatcacgcct
    36181 gggatgccca cgaaaaaggc gaaaaagcgc agcagaggtg gaatgagaag tttgccgcct
    36241 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg tcggatgagc ggtggtttac
    36301 cgaaggactg ggagaaaacg actcagaagt atatcaatga gttgcaggcg aatccggcga
    36361 aaatcgccac ccgtaaggct tcgcaaaata cgcttaacgc ttatggcccc ttgctaccgg
    36421 agctgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
    36481 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
    36541 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
    36601 tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
    36661 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
    36721 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
    36781 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
    36841 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
    36901 aagagattac tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
    36961 tgattgccac cggttcagag atggaaatca ccctgcaagc tgcggagaaa ttagcaggag
    37021 aaggtcgcaa tgttcgcgtg gtttccctgc cctcgaccga tattttcgac gcccaggatg
    37081 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
    37141 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
    37201 gttatgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
    37261 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatag tgatcgccgg
    37321 atgctgattg ccggatgcga cgctgacgcg tcttatccgg cctacagcgt cttatccggc
    37381 ctacatgtcc ccgccatttt gtttaacggg tgatccacaa cgtgggccag gcgtctggcc
    37441 catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt
    37501 caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca
    37561 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
    37621 gcccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg
    37681 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagctg atcatcatca
    37741 gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
    37801 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg
    37861 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
    37921 cgctgagcgg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
    37981 gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg gtctgattgc
    38041 cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg gtgatggttg
    38101 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca
    38161 acggctcacc atctaacagc agccgtggcg ctatctctcc ttcagacgca tccggcgact
    38221 tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca
    38281 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag ttttggttat
    38341 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctag tgccgcccac accaaagacg
    38401 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
    38461 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
    38521 agatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt ttcagtccat
    38581 taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
    38641 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
    38701 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
    38761 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
    38821 cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt
    38881 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
    38941 agcgacacgg aactgacgaa tcagaaccac ggtctttttc ttcgcgttgt acaggaggat
    39001 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt agccatcttt
    39061 gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
    39121 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat
    39181 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
    39241 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
    39301 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
    39361 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
    39421 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
    39481 gcatgacaaa ttcactgccc tcaatcggta caagtcgccg gcgcccctgg gcatccggct
    39541 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa
    39601 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
    39661 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
    39721 cgccgtggcg cagcgcggta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
    39781 cgacgttgag tccggcggta ttgataaacg gctcttccgg tagctcttcg agtcccatca
    39841 cctgtttagt gttggcgatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
    39901 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
    39961 gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
    40021 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
    40081 aaggaatgcc gaaagtgagc aagccaccaa tttctggatg gcgatcgtac accgtcaccg
    40141 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggaccc gcaccgataa
    40201 tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa cctttcgcca
    40261 acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc
    40321 gaatagtaca ggcaccttca cacaaacggt cctgcggaca aacgcgtccg gtaatttccg
    40381 gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
    40441 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
    40501 cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg atcggcacgg aatggcagat
    40561 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
    40621 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac
    40681 tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc
    40741 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg
    40801 caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc
    40861 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg
    40921 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
    40981 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
    41041 tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
    41101 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
    41161 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
    41221 accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg gcaatgttca
    41281 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
    41341 tgactaaatt gcgttttagc gaactggaac attaatgatt ttttgtggag aagacgcgtg
    41401 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
    41461 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
    41521 aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc
    41581 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcat
    41641 cggtattaac caatctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    41701 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    41761 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    41821 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    41881 ttttcacgct ggtctttttt actttgcgcc gtatacgcca tcaggtggtt gccccgctga
    41941 atcagctggt taccgccagt caacgtattg aacacgggca gttcgactcg ccgccgctgg
    42001 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag atgtcgagcg
    42061 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    42121 acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc gcaggcgctg aacacaagcc
    42181 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcggctg
    42241 aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa ccaaacccgg
    42301 aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
    42361 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg tcgatgctgg
    42421 gacgcggttt gtactttaat caggcgcaaa agcattttca gcagttattg ttgatggaag
    42481 aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt tcttacttac
    42541 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
    42601 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
    42661 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
    42721 atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg ccaaccctgg
    42781 cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gtgctgaatg
    42841 cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg ccggacggca
    42901 atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa gaacccgaag
    42961 gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctgggtggg acgctgactt
    43021 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctct gcggagggtg
    43081 aggaaagtca gttgatgtaa tgcctcctac tgaccaaaga atacttgcac ttaaggttca
    43141 gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc
    43201 ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg aggtcctctt
    43261 ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg
    43321 tgtctgacag ggaccctggc gattttttca ttgcccgttg aacaataccc cgatctcgcg
    43381 ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac
    43441 accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat gtatatgtca
    43501 tctcagagca gtggcatcgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat
    43561 ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg
    43621 caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa tattctgacc
    43681 attgctttcg tctctaccga tggttcgatg gataaacagg atattgccga ttatgttgcc
    43741 agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt
    43801 tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg
    43861 gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt
    43921 ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg
    43981 ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcaaga cggctcagag
    44041 gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc
    44101 cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg cgcaaacgaa
    44161 atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta cttcccgcac
    44221 ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac
    44281 gtggtgaaaa cgctgctgga agctatcgcc ctggttttcc tcgttatgta tctgttcctg
    44341 caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc
    44401 ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggta
    44461 ctggcgatcg gtctgctggt ggatgacgca atcgtggtgg tggaaaacgt cgaacgtatt
    44521 atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag
    44581 ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtaccaat ggccttcttc
    44641 ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg
    44701 ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacact acttaagcca
    44761 ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa ccagatgttt
    44821 aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg cagcctgcgc
    44881 tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg
    44941 acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca attgcccagc
    45001 ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcacc
    45061 catgaaaaag acaacatcat gtcggtgttt gccaccgttg gttctggccc tgggggtaac
    45121 gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag
    45181 accggcacct cgtttgccat tatcgagcgt gcaacaaagg cgtttaacaa gattaaagaa
    45241 gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc tgcgggtttt
    45301 gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc agcacgtaac
    45361 cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc
    45421 gacgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt
    45481 gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac
    45541 tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg
    45601 ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc
    45661 tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc gtctggaacg ctataacggt
    45721 tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg
    45781 gatattatgg aatcgttagt gaagcagctg ccaaacggct ttggtctgga gtggacggcg
    45841 atgtcgtatc aggagcgact ttccggcgcg caggctccgg cgctgtacgc catttccttg
    45901 ctggtggtat tcctgtgtct ggctgcgttg tatgaaagct ggtcggtgcc gttctcggta
    45961 atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg caacctggat gcgcgggctg
    46021 gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac
    46081 gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa
    46141 gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt
    46201 atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg
    46261 gtgggtactg gcgtaatggg cgggatgatt tcagccacta ttctggctat ttacttcgtg
    46321 ccgctgttct ttgtgctggt gcgccgccgc ttcccgctaa agccgcgccc ggaataagca
    46381 ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc
    46441 agcgggttct tgctgtttat actttgtctc aggaattact tacgaagcat aacttcgata
    46501 aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt
    46561 attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc
    46621 tccgggggga caaatcttat gtaaatacta tggtcctgcg gtgatgattt gtatgtgata
    46681 cacagcaaca tttcgagata ttcatacggc atctaatact gatttaattc aggtt
//
LOCUS       DAZOYS010000009        46226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOYS010000009 DAZOYS010000000
VERSION     DAZOYS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.009"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHUN9_000411"
     CDS             complement(<1..264)
                     /locus_tag="ACHUN9_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0693924.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHUN9_000412"
     CDS             complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHUN9_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0693925.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            839..3037
                     /gene="priA"
                     /locus_tag="ACHUN9_000413"
     CDS             839..3037
                     /gene="priA"
                     /locus_tag="ACHUN9_000413"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0693926.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASEIPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3193..4218
                     /gene="cytR"
                     /locus_tag="ACHUN9_000414"
     CDS             3193..4218
                     /gene="cytR"
                     /locus_tag="ACHUN9_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0693927.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHUN9_000415"
     CDS             4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHUN9_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0693928.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5362..5892
                     /gene="hslV"
                     /locus_tag="ACHUN9_000416"
     CDS             5362..5892
                     /gene="hslV"
                     /locus_tag="ACHUN9_000416"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0693929.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5902..7233
                     /gene="hslU"
                     /locus_tag="ACHUN9_000417"
     CDS             5902..7233
                     /gene="hslU"
                     /locus_tag="ACHUN9_000417"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0693930.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7300..8226
                     /gene="menA"
                     /locus_tag="ACHUN9_000418"
     CDS             7300..8226
                     /gene="menA"
                     /locus_tag="ACHUN9_000418"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0693931.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8319..8804
                     /gene="rraA"
                     /locus_tag="ACHUN9_000419"
     CDS             8319..8804
                     /gene="rraA"
                     /locus_tag="ACHUN9_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0693932.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHUN9_000420"
     CDS             complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHUN9_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0693933.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9560..10405
                     /gene="glpF"
                     /locus_tag="ACHUN9_000421"
     CDS             9560..10405
                     /gene="glpF"
                     /locus_tag="ACHUN9_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0693934.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10428..11939
                     /gene="glpK"
                     /locus_tag="ACHUN9_000422"
     CDS             10428..11939
                     /gene="glpK"
                     /locus_tag="ACHUN9_000422"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0693935.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12069..13079
                     /gene="glpX"
                     /locus_tag="ACHUN9_000423"
     CDS             12069..13079
                     /gene="glpX"
                     /locus_tag="ACHUN9_000423"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0693936.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13176..13922
                     /gene="fpr"
                     /locus_tag="ACHUN9_000424"
     CDS             13176..13922
                     /gene="fpr"
                     /locus_tag="ACHUN9_000424"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0693937.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHUN9_000425"
     CDS             complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHUN9_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0693938.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14382..14681)
                     /locus_tag="ACHUN9_000426"
     CDS             complement(14382..14681)
                     /locus_tag="ACHUN9_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0693939.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14893..15333)
                     /locus_tag="ACHUN9_000427"
     CDS             complement(14893..15333)
                     /locus_tag="ACHUN9_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0693940.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15434..16033
                     /locus_tag="ACHUN9_000428"
     CDS             15434..16033
                     /locus_tag="ACHUN9_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0693941.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHUN9_000429"
     CDS             16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHUN9_000429"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0693942.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHUN9_000430"
     CDS             complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHUN9_000430"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0693943.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHUN9_000431"
     CDS             complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHUN9_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0693944.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHUN9_000432"
     CDS             complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHUN9_000432"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0693945.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHUN9_000433"
     CDS             complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHUN9_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0693946.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHUN9_000434"
     CDS             complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHUN9_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR0693947.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21714..22877)
                     /locus_tag="ACHUN9_000435"
     CDS             complement(21714..22877)
                     /locus_tag="ACHUN9_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR0693948.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22877..23356)
                     /locus_tag="ACHUN9_000436"
     CDS             complement(22877..23356)
                     /locus_tag="ACHUN9_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0693949.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23371..25818)
                     /locus_tag="ACHUN9_000437"
     CDS             complement(23371..25818)
                     /locus_tag="ACHUN9_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0693950.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25811..25966)
                     /locus_tag="ACHUN9_000438"
     CDS             complement(25811..25966)
                     /locus_tag="ACHUN9_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR0693951.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25963..26238)
                     /locus_tag="ACHUN9_000439"
     CDS             complement(25963..26238)
                     /locus_tag="ACHUN9_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0693952.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26295..26813)
                     /locus_tag="ACHUN9_000440"
     CDS             complement(26295..26813)
                     /locus_tag="ACHUN9_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR0693953.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26826..28016)
                     /locus_tag="ACHUN9_000441"
     CDS             complement(26826..28016)
                     /locus_tag="ACHUN9_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR0693954.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28427..29845
                     /locus_tag="ACHUN9_000442"
     CDS             28427..29845
                     /locus_tag="ACHUN9_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693955.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29960..30487)
                     /locus_tag="ACHUN9_000443"
     CDS             complement(29960..30487)
                     /locus_tag="ACHUN9_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0693956.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30491..32809)
                     /locus_tag="ACHUN9_000444"
     CDS             complement(30491..32809)
                     /locus_tag="ACHUN9_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0693957.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32820..33350)
                     /locus_tag="ACHUN9_000445"
     CDS             complement(32820..33350)
                     /locus_tag="ACHUN9_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR0693958.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33343..34251)
                     /locus_tag="ACHUN9_000446"
     CDS             complement(33343..34251)
                     /locus_tag="ACHUN9_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR0693959.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34256..34603)
                     /locus_tag="ACHUN9_000447"
     CDS             complement(34256..34603)
                     /locus_tag="ACHUN9_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR0693960.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34600..35235)
                     /locus_tag="ACHUN9_000448"
     CDS             complement(34600..35235)
                     /locus_tag="ACHUN9_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0693961.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35302..35754)
                     /locus_tag="ACHUN9_000449"
     CDS             complement(35302..35754)
                     /locus_tag="ACHUN9_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR0693962.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35747..36214)
                     /locus_tag="ACHUN9_000450"
     CDS             complement(35747..36214)
                     /locus_tag="ACHUN9_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0693963.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHUN9_000451"
     CDS             complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHUN9_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR0693964.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHUN9_000452"
     CDS             complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHUN9_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR0693965.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36735..37160)
                     /locus_tag="ACHUN9_000453"
     CDS             complement(36735..37160)
                     /locus_tag="ACHUN9_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR0693966.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37175..37672)
                     /locus_tag="ACHUN9_000454"
     CDS             complement(37175..37672)
                     /locus_tag="ACHUN9_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR0693967.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37672..37953)
                     /locus_tag="ACHUN9_000455"
     CDS             complement(37672..37953)
                     /locus_tag="ACHUN9_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0693968.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37957..38160)
                     /locus_tag="ACHUN9_000456"
     CDS             complement(37957..38160)
                     /locus_tag="ACHUN9_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR0693969.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38160..38669)
                     /locus_tag="ACHUN9_000457"
     CDS             complement(38160..38669)
                     /locus_tag="ACHUN9_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR0693970.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38769..39512)
                     /locus_tag="ACHUN9_000458"
     CDS             complement(38769..39512)
                     /locus_tag="ACHUN9_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR0693971.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39516..40588)
                     /locus_tag="ACHUN9_000459"
                     /pseudo
     CDS             complement(39516..40588)
                     /locus_tag="ACHUN9_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40647..41501)
                     /locus_tag="ACHUN9_000460"
     CDS             complement(40647..41501)
                     /locus_tag="ACHUN9_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR0693972.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41675..43447
                     /locus_tag="ACHUN9_000461"
     CDS             41675..43447
                     /locus_tag="ACHUN9_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR0693973.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43447..44481
                     /locus_tag="ACHUN9_000462"
     CDS             43447..44481
                     /locus_tag="ACHUN9_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0693974.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44853..45995
                     /locus_tag="ACHUN9_000463"
     CDS             44853..45995
                     /locus_tag="ACHUN9_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0693975.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45997..>46226
                     /locus_tag="ACHUN9_000464"
     CDS             45997..>46226
                     /locus_tag="ACHUN9_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0693976.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIH"
BASE COUNT        10881 a        12419 c        11878 g        11048 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttc gcaccgagca gcggattgac
      121 cggatggccg gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc
      301 ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt
      361 tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg
      421 gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt
      481 agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt
      541 caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt
      601 aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc
      661 gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg
      721 atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc
      781 gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat
      841 gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc
      901 agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga
      961 gcgcatcggg gttgtggtat cagttagcga tgtcagtgag ctgccgctta acgagctgaa
     1021 agcggtagtt gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcgattact
     1081 gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc
     1141 gattttactg cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac
     1201 tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcacccaagc aacaacaggc
     1261 gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag gttgccacgc tcgaatttaa
     1321 tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaatacc
     1381 agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac
     1441 cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct
     1501 gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt
     1561 gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac
     1621 tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt ccggtctgaa
     1681 cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat ggcgaggcgg cgattgtgat
     1741 cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgacga
     1801 agagcacgac agttcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc
     1861 ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct
     1921 ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc
     1981 ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc
     2041 aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt
     2101 tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta ctgtgccacg actgcggctg
     2161 gattgccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt
     2221 gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc
     2281 cacgcacctg gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct
     2341 gttccctgat gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct
     2401 ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat
     2461 gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg
     2521 cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt
     2581 cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc
     2641 ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc
     2701 gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc
     2761 ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct
     2821 ctccagcccg ctggcagacg acaaattgtg ggttctcggt ccggttccgg cactggcacc
     2881 taaacgtggc ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt
     2941 gcaacacatc attaacggta cgctggcact catcaataca ataccggatt cccgtaaggt
     3001 gaaatgggtg ctggatgttg atccgattga gggttaaacc gctcacgatg cgaggcggat
     3061 cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta
     3121 tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg
     3181 cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc
     3241 ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc
     3301 tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag
     3361 cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat
     3421 atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac
     3481 ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc
     3541 gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt
     3601 gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca
     3661 ccggaactgg agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta
     3721 aattatctat atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg
     3781 ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg
     3841 gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg
     3901 cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg
     3961 gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc
     4021 ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc
     4081 gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag
     4141 gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca
     4201 acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg
     4261 cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg
     4321 agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa
     4381 aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgtgct
     4441 tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac
     4501 gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg
     4561 ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc
     4621 cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca
     4681 aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca
     4741 gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga
     4801 gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg
     4861 tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca
     4921 gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca
     4981 gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga
     5041 cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt
     5101 acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa
     5161 tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt
     5221 gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa
     5281 tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat
     5341 tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg
     5401 gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa
     5461 aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat
     5521 gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc
     5581 aaagccgccg ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg caaacttgaa
     5641 gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg
     5701 gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg
     5761 gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga aaaggcgttg
     5821 gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc
     5881 tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg
     5941 gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac
     6001 cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc
     6061 ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg
     6121 gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctatgtcggt
     6181 aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc
     6241 caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac
     6301 gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc
     6361 gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc
     6421 gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa
     6481 gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg
     6541 cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg
     6601 gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg
     6661 tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct
     6721 cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa
     6781 cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg
     6841 aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc caatccgcgt tgaactgcag
     6901 gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg
     6961 cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt
     7021 aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt
     7081 cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta
     7141 agcggtcaaa atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg
     7201 gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt
     7261 tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc
     7321 cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca
     7381 attatcgtcg ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg
     7441 ctggtactaa ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc
     7501 gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa
     7561 ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt
     7621 ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc
     7681 ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct
     7741 tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagcgtc
     7801 atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc
     7861 gcctgtggcc tgctggcaac ggcagtactg aacattaata acctgcgtga tatcaatagc
     7921 gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt
     7981 tatcatgcct gcctgctgat gggctctctg gtatgtctgg cgctgtttaa tctcttttcg
     8041 ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc
     8101 cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc
     8161 aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca
     8221 gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta catagcgcga
     8281 tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc
     8341 tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg
     8401 gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc
     8461 tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt
     8521 ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg
     8581 aaggtctggt tatttacggc gcggtgcgtc aggtagatga cctagaagag ttggatatcg
     8641 gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg
     8701 atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt tatgccgaca
     8761 ataccgggat tattctttca gaagatccgc tggatattga atgataagaa aggcaccgca
     8821 aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg
     8881 aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg
     8941 ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca
     9001 ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc
     9061 aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct
     9121 aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc
     9181 cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa
     9241 gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc
     9301 cttctcctgg tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg
     9361 atctaacaaa catgcatcat gtacaatcag atggaataaa ttgcgcgata acgctcattt
     9421 tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag
     9481 tttacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt
     9541 aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga
     9601 attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag cactaaaagt
     9661 cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat
     9721 ggccatctac ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc
     9781 attgtggctg tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt
     9841 tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt tatttttcga
     9901 cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa agtgttgatc tggcaggcac
     9961 tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt
    10021 gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc
    10081 acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat
    10141 gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc
    10201 ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct
    10261 ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact
    10321 gattggtcgc catttgcctt gcgatatctg tgttgtggaa gaaaaggaga ccacaactcc
    10381 ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa
    10441 aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg
    10501 atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggct
    10561 gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc
    10621 tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg
    10681 aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc
    10741 agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata
    10801 tccgcagcaa taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga
    10861 tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg tggtgaattg ctgtttggta
    10921 cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca
    10981 ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc
    11041 tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtgt
    11101 acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg
    11161 gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca
    11221 cctatggcac tggctgcttt atgctgatga acaccggcga gaaagcggtg aaatcagaaa
    11281 acggcctgct gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgctggaag
    11341 gtgcggtgtt tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta
    11401 acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg
    11461 tggttccggc ctttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga
    11521 ttttcggtct gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta
    11581 ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc
    11641 acgccctgcg cgtggatggt ggcgcagtag caaacaattt cctgatgcag ttccagtccg
    11701 atattctcgg cacccgcgtt gagcgcccgg aagttcgcga agtgactgca ttgggcgcgg
    11761 cctatcttgc tggtctggcg gtcggtttct ggcagaacct cgacgagctg caagagaaag
    11821 cggtgattga gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg
    11881 caggctggaa aaaagcggtt aaacgcgcga tggcgtggga agaacacgac gtaatgtaaa
    11941 tgccggatga agcgtttatg ccgcatccgg tagtcccgaa acgtgcgggg gcaaccccgc
    12001 acacatcaat aatccctccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
    12061 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc
    12121 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
    12181 acgccatgcg tattatgctc aaccaggtca atattgacgg caccatcgtc attggtgaag
    12241 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
    12301 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggccagg
    12361 ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat gcgccggata
    12421 tgtatatgga gaagctgatc gtcggaccgg gagccaaagg caccattgat ttgaacctgc
    12481 cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg caaaccgttg agcgaactga
    12541 cggtaacaat tctggctaaa ccacgccacg atgccgttat cgctgaaatg caacaactcg
    12601 gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc acctgtatgc
    12661 cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc gtagtttctg
    12721 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
    12781 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
    12841 cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc agcgataacg
    12901 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
    12961 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc attcgccgca
    13021 ttcagtctat ccactatctg gatcgcaagg acccggaaat gcaggtgcac atcctctgat
    13081 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
    13141 aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
    13201 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccccgtgc
    13261 ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc gaacgcgtcc
    13321 agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
    13381 ccgtccccga tggcaaatta agcccacgac tggcggcact gaaaccaggc gatgaagtgc
    13441 aggtggttag cgaagcagct ggcttctttg ttctggagga agtaccagat tgcgaaacgc
    13501 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggca
    13561 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
    13621 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc
    13681 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata ccggcattaa
    13741 ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
    13801 tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg aaagagaccc
    13861 ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag cattactggt
    13921 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct tattgatcat
    13981 cccgcgatat gccggcatca aacggttgat aaatgagtgg tgatgaccac agacaaggag
    14041 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
    14101 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
    14161 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
    14221 ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
    14281 ctccagggct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
    14341 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
    14401 gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc atcaagctca
    14461 acttgcccct ccagatcctt aatcttatag gccacgctgg ccccttcatc tgcagaggat
    14521 gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc gcgggtggca
    14581 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
    14641 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
    14701 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
    14761 tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc agaaggattt
    14821 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
    14881 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
    14941 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
    15001 caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac ccggtaaaat
    15061 cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc atgcgccgga
    15121 acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa gcaagcacac
    15181 aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg agaaaagcac
    15241 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
    15301 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
    15361 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
    15421 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
    15481 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
    15541 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
    15601 gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
    15661 tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag cgctggagcg
    15721 cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg ggccagagca
    15781 aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca cactcacccc
    15841 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctactaacgg cggggaaaaa
    15901 caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg acaacggcga
    15961 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
    16021 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
    16081 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
    16141 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
    16201 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
    16261 gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag ccacatcatg
    16321 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacttctgcc
    16381 gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga acgtcgtact
    16441 taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcagggc
    16501 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
    16561 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
    16621 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
    16681 gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt tgacgctaac
    16741 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
    16801 ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct gaaagctgac
    16861 gctttcgcag tgatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
    16921 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
    16981 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
    17041 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
    17101 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
    17161 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
    17221 caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca catcaggacg
    17281 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
    17341 caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa aatcacgcgc
    17401 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    17461 accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa gatcttttaa
    17521 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    17581 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    17641 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    17701 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    17761 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg
    17821 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    17881 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    17941 tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    18001 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttggtgcctt
    18061 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    18121 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    18181 cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg gagccgcgcg
    18241 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    18301 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag ttccagcgtg
    18361 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta atcagatcgt
    18421 tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg gtggaagtgt
    18481 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    18541 caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    18601 ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc acgttatcac
    18661 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    18721 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg gttgccgcca
    18781 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    18841 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    18901 aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc atattgctga
    18961 caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaagtt
    19021 tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc atcaggcatt
    19081 tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa tcattaatac
    19141 agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt ttcgatagcg
    19201 tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg accgccgtaa
    19261 cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat acggtcgtaa
    19321 ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt ttcacgaccg
    19381 gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc ggtaatcgcc
    19441 acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac caggtcttca
    19501 cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat ggccgcagcc
    19561 aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg ctggtgagaa
    19621 gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc agtgaagaaa
    19681 ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag accgatgcac
    19741 gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc gccgataacc
    19801 accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg gatgttctcg
    19861 tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt gatcatgtca
    19921 gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag atagccgtca
    19981 taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc gcgaattgcg
    20041 gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt aatcatgact
    20101 acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt gcacatcgaa
    20161 gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg attcaggtca
    20221 ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta tactgattat
    20281 gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg atggataatt
    20341 acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc caccatatgt
    20401 gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa gcgggtcggc
    20461 cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga agtcacgata
    20521 tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga ctgtaccgcc
    20581 tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa tgcaaacaga
    20641 gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat tgcgccgttc
    20701 atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct ttgcgtccgg
    20761 cgcaccaccc accgctgaaa cgagacaagg ataatcgtac aaattagcgc cacaattgtc
    20821 acgataaccc cgacgcctgg atctgtcatc ggtgttggag ataccagatg ttgaataccc
    20881 gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag cgccgcgagg
    20941 gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag ggaatatcgc
    21001 accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac cagcgcggcg
    21061 agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa tagcagcgaa
    21121 gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga ttgattcata
    21181 aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc aatgcattaa
    21241 gcagcaggca aattgaggat aaaaaaaccc ccacatcatg tgggggaaga cagggatggt
    21301 gtcacaaaaa agtacctaac ctgatgatat aaatggattt atgtcaaccg ctgtccacat
    21361 agcgtccaca tcgaccataa ataagcccct caactgaggg gctatttttt tgatcacatc
    21421 cacataattt gctgccctga cggcaacggg tgcggcctta cggcgtggac ttctcccggc
    21481 ttcacgatgt atcgctgtac cgactcataa gtgatgaacg tggcgctgca attcacgttc
    21541 tggcactggt gataacgctc ttttgtcgtg tcagtgatat agcgacttgt acgcgcatgt
    21601 gcggcatgct ggcataaagg acaatgaaac atcgcgagca cctcttccgg ttttgttgat
    21661 ggtgccattt tagttaattt atccttataa aacaaacaga taaataaacc acgtcactca
    21721 tcatcttctg tttcgtactc cacatcagaa agccttacct caagctccaa ggacgtcgtg
    21781 aagccgctat tattgagaca atgtgtcacc ttagtgatgg tccagtcctg ctcgtctatg
    21841 acgcgcttaa agcctgacac tttaaccggt gtttccgtgt aaatatctgc acgaccggta
    21901 gccaggctga tggagaactc cgctacaccc cgttgcagtt tatcccactt cgcctgagcg
    21961 gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca gggcaaaaac gttgtcagct
    22021 tcaccggcca tgtattcacc ttcgcgcgct tccggtactt ttggcgcttt cttctgcgtg
    22081 accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt tttctttctt tttgcgtttc
    22141 agttttacct tctgcttttg cggcttcggg tctttggtgt gtaaccactt tgccgttacg
    22201 ccggtgtaag ctccacggtc agcaatcgca aaatgatgac ggtcgccgtc gctgcgggtg
    22261 atggtaatct gcgggatttt tttaccgctg gccgtcaccc cctgccccgc tttgagaaac
    22321 agcagttttc ccatttttac cgacacctcg ccgccgttgc gttcagcaag acgggtcagg
    22381 aatttcgcat cagactcctg cgactggtcg atgtgtggaa tttttattcc ggccagtgac
    22441 ggagcgacac tggcttccag cctgttacgg gaggctatcg cctcaacaat cgcaccgagc
    22501 gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg tcccgcgaaa atctgcactc
    22561 cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt caacctcatc aacggtaaat
    22621 ttccctttgc ataccagggc aaaacctttc cagccgatat acaccgtcag gacagcgcca
    22681 cgaaccggca gcccgacctg cccgtcggca tcgttcagtt caatatcaag ctggtcagcc
    22741 tcaaagcccc ggttatccgt cagggtcatg ctcatcagac ggtcgctgat attgccggta
    22801 atatccctgc tgtcgagcat cagcatgtaa tccggcgtca gcgtactgcc tgcatcaaat
    22861 gtcagcgcat ccagcattat cccgcccccg tcatacccgt gaatttagtc gccatactgc
    22921 cagccttacc gatgagcgat tccgcctgtt taccgatatc gccataaagc gcggccagtg
    22981 actcatcaac gcgggtgagc gacagcgtaa aatcaatttt ccgtggtgtg ccgtctgcaa
    23041 agaaaatact ccctgtttca ctcaccttgc tgatgacata catgccgtaa atcatgccgg
    23101 tgccatccag caacggccac gcccggcctt cctctgccat cagtctgagc gtggtcatcg
    23161 tcagctttcc gccagtcagt tcgggataaa gcacaccggc aagtgtgatg ttctcctcac
    23221 ctacaccgag aaactgatag gcatcccgtt taccgatacg ggaatttgac ggccagcgat
    23281 aatctgattc acgctgcatg gtctggtgtg gcagcgtctg gcgcataaaa acaaacatac
    23341 ctaacgcgag catcattttt cgtcacctcc ttaaccgtca tgcatcatgc tggcacgggc
    23401 gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc tgtaactggc ggtcaagctg
    23461 tgtccccggt gcagtaccac ccgtcaggct gatgtgatat tcgtttttac tctggtccac
    23521 ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc tgatagcctg cataagagct
    23581 ggtcgccgga atataaccac cggtgccata cgtggcggca tgagttctgg cggcggtctg
    23641 gtcaagtgtg tctgactctt tgttgatgac cccgagcttt tccagtaccc agtcaatgcc
    23701 gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc gcgtcagcca gtgcctgccc
    23761 gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc tgggtggctt tgaccggggc
    23821 aatcaggttt ttaaaccact gccacgcagc ctgtaacttt tcgcccagcc agtcaaacac
    23881 cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat gccgctttca gcccttcaac
    23941 cacaccgcca aagaatgcgc tgacaggctc ccagtattta cggataagca acgccccggc
    24001 gacaatggcg gccaccacag ccacaaccgg ccagctaatc gccccgatgg ccgtcataac
    24061 ggcactacca accgtcgtga agattgcccc cattgcgcct gctgccgcga tgatggcatt
    24121 gatgccggtg ataaccggcc aggctacaag accaattgca ccgatgatgc cagtcagcgc
    24181 cagcgcgcca ccggcaatga ggccaatggt tgacgccagt gatttgtttt tctgtaccca
    24241 gccgtcgagt tttaacacat actttgtggc cgtctgcgtg agcttacgta gcgcgccttc
    24301 ctgctggtca aacaggtctg tccccaccgc ctcataagcg gactgaaact ccttaaagtc
    24361 accgccgagg ttgtcctgca tgatatttac cagctctgcg gtcttcccgt ctgaggcttt
    24421 aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg gcagtcatca gcacagcggc
    24481 ggctgagctg gcctcctccc cgaaaatggt tttcatgtat tcggcctgct gggcagtacc
    24541 gagccggttt ttctcaaagc tggcctgcat ttctttcaga atggtaaata ctggtcgggt
    24601 gtttccctta ctgtctgagg ttttcacgcc aagctctttg agtgcatccc atgcttttcc
    24661 agtcggtgcc tgcaggcgac ttaacacggc acggcttccc gtccccgcca ttgagcctgt
    24721 gatttttgca tcatgcagcg ccccgaccat tgcggcggtt tcttcaatgc tgacaccggc
    24781 attttttgcc actggtgcgg cataggtcag cgcatcgctc atgccgtcaa aatcagcggc
    24841 ggttttgttc atcgtcatgg agagaacatc cccgatatga gcgaccttat cgtttgaaag
    24901 ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt tcttccattg tgcggcggtt
    24961 cgccagcgcc atattcagcg tgaccggcgt tgccgcctga atggcatcaa catccccacc
    25021 cgctttcgca atgataatct gtgcaccggc cgcatcatcc gctgaggcgg cggtgttgtc
    25081 accgagctgg cgcgcctgct tgcggagtgc agccatttcg gcggagtctt ttgccacacc
    25141 gagcacagcc tgcaattctg agtttttctg cgcaaactca taacctggca tcagcagctt
    25201 aactccggct atcgttcccg cagcagcaat ccccacaccg gcagcgccca ctgaggccat
    25261 atttccggcc agttcctttc cggcctgata acgctgtttg actgcgttaa gttttgcctg
    25321 ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta agctgggcgg tggtttcact
    25381 gatacggttt ttcagtccct gctcatcatg tgcaagattg cgggtattaa ttcccacagc
    25441 ggccagttcc cgctgctggc gtttaacgga atctgtcagg cggttatatt tcgcctgtaa
    25501 gtcctccgcc gcacgctttg cggattccag cactttcgcc tgagcacggg tcgggcgttc
    25561 ggtgttttta aactgtgtgg caagggcttc ggcctcctgc cgtgcctttt caagtgcatg
    25621 accagtcacg gcgagctgtg cactggtctt gcggaatccc tcaatacggg atgcgtgacc
    25681 gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc cccgacagca acttactcgc
    25741 tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca gccctgagca atacctgtaa
    25801 ttttacattg ttactcattc gtgtttccgc ttcgcctgag cgccttttcg cgccatgtga
    25861 tgagttcggt caggctcatg ggatacagtt ctgatggcgg ccagtgaaat atcactgcca
    25921 catccgccat caggtcatcg accgacagat ttttcggaag cgtcactgca ccgagttcgg
    25981 cgacaaaaaa ccgaccacct taccggccag cgccacaagg tcaggcagtt ccagcgcggc
    26041 gacttcctgc tcggtcagca tcggtgccgt catgcgcggc agcaccttaa tcagtgcatc
    26101 gacttctgag tttgcgaccg cagccagact gacaccgcgc agcgtccccg cactgggttt
    26161 catcagcgtg acctgttcga taacctgctc accacgcttg accggattgt ccagggtaat
    26221 cacattttct ttgttcatgg ttttctcact tctgaatcgg ggttaaccgg tcagccaggc
    26281 tgaccggatg aaaattacag gccgatattg cggcggtgtt gctccagccg gtcgacgccg
    26341 ttcaccttct caatcatgtt gatggtgtcg atttcgacca gctccttacc gtccatcgtc
    26401 agccggaaat aggtgcagac cacggagatt ttcgactcgg tgtcttctcc ctgtttaccc
    26461 tcgccggtgt cgatttcttt ctgacggcca cgcatgacca cctctacggc caccgtttcg
    26521 ccggtatcgt cacgctggta agagcctgca aaacgaatcg gcacggcatc cacaccggtt
    26581 gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc cgagcgacca ctccattgac
    26641 agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc cgttcatccc cgcaccgcga
    26701 tagttttcga gtttacgggt cagttttggc agcgtgacgg actttgcaac gccctgatag
    26761 ctgtagccgt tcagaaagac gttcattaac ttgagtttgc gcggcattgc catcggtcag
    26821 gctccttaat tgctgttaac cgaggtgacc agatttgcca ggtatttatc ggtaatacgc
    26881 tggcgcaggg tcaggttttc aagaggaggc accggggtat agtcgtagtc gatatacagt
    26941 tttccggcct tgagggtttc cgcatcgttg gattcttcgc tgaaccagca ggtcgcatcc
    27001 acgatatagc cgtttgtttt cagctcacgg aatttggcat tgatgccgtc aacgatgtcg
    27061 cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca tgtgcgcctc agccatcgtg
    27121 tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga ggaacagcgg gtcatcagag
    27181 caggtacggt taccccagaa gcggaaaccg tcacggcgaa tcagcgttgt gacgcctgac
    27241 tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat cccagaatac ggacgcgctg
    27301 atgccggtaa caccgttcac cccgacgttg gacagcgttt tatgccagcc ctgctcctgg
    27361 tcgattttag cgcgcagacc cagcgcacgg gcggtggcat acgcggtggc ggtggtactg
    27421 gcgaccgtat cccatgcgag gaaatccggc cagatgacca tcagctcacg ctggctgaaa
    27481 ttctgacggt aggctttcac ctcggaaacg gtcttacagc cccatgcgct gatatacccg
    27541 aaagcgcgca gcttctgaca gactgatgcc agtgcaacag ccacctcttt ggtgtccagt
    27601 cccggcacgc cgagaatacg cggtttaaca ccggttaccg actccgccgc cagcagggct
    27661 ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga tgatattgga aacggtctgc
    27721 gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca cacgcacgac aacggtgacc
    27781 ggttttgact ggtcggcgat ggcctgcaac gatgccgcca gcgtgccttt tttaccggcc
    27841 tttgcaattg cgctctgcac attggtaatc agcaccggtt tattgagggg gaaggtttcc
    27901 gcatccgcat cgctggccgt gcagaccatg ccgacaatgg cagtggatac ggtggaaatg
    27961 acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt gatgatagtc actcatccgt
    28021 ttaactccgt ggttaatggg tgcaactatt ttctgttggg cagtgcatga gacgctattt
    28081 gacctggctg gtcagtggat gaaacaacag ataaagaaaa tgcgggcaat tcgcccgcat
    28141 gtcctgattt gtacacactc attttccgac tgacagttta catagcccaa acgctatcaa
    28201 atctgacagc ctgctatgag cgaaaagcgt taaatctcac gatatctgct accgggaaaa
    28261 atttcgatta tcttcgatgg gaactttgat aatgtagtaa atgccatttt cagcgatcac
    28321 ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac ttcactgaaa taaggttgct
    28381 agtatttttg gagcaaccat taatgttttt tatttaagga atgtaaatga catacgcact
    28441 gattgataat tcaactctaa ctgcagtcca aagaatcgag gggaaagtaa aaacaagatc
    28501 gaaggactca gtagatacag acataatagc atttgagaac tatatacagt ctattttgtt
    28561 ctatgatcac gttatcgcgg ttgatgatta tattccagag taccgtggga gtaggatttc
    28621 aagtttcaga gagatattat ttcttgataa agcacaatat gggttggatg aaattgaaag
    28681 tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt cgtggtggaa catttgttaa
    28741 tgacgatttt aaaaaactaa ttgaacttct tcagacacat atcatatgta catgggacat
    28801 tagttcaagt gtttatcatc taacactgaa gaacttatct gatggaggta aggagtttga
    28861 aaaatatggt aacattgctg ctgccatttt ttcagaactg cgtgatgctg ctgagtctgg
    28921 taatagaaca aatggcgatg ttgaacttct cgatcgattt ggcaacccta ttcgcaaggg
    28981 gtacaaagtt cccggagcaa aatgggggga tggcacatct gctggtgaag catcagatgc
    29041 aatcaaagca ttcgtggctt cgcttgtatg gctggctaat cgggcagtat tttattctct
    29101 cacagctaag taccttcaag cagatacttt tttatatcca ttacgtcagg cgtatcaaca
    29161 gacatattta agccaaaact gtcagtatgg ttttgactat gctaaacata ttgtagaaaa
    29221 cttttccact tcgttaagtc atgatctcat tgatattcat tctggtggta aagctgtagc
    29281 aacagcaact tcgcttcctt tattttcggc atggctcgca aaagaaactg gtgatccaag
    29341 ttgtattgtt gaagctgctt ataacataag ggacaacaac gaatttgtaa gggcccgtga
    29401 gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa ttgcctcaag ctaataagtc
    29461 agtaagagca ttgcttactg aagtatcgaa agaatcagat tatattcgaa ctaagtatgg
    29521 gctcaagact agacaaggtg tacctgtaac taagcttgta catgtataca atactgttgc
    29581 ggctattaat tcccttccga aactaccgac ctttaatttt aaagttaaag ttcctgattt
    29641 cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc tatcgaaatt tgactaatga
    29701 tctttctact gtgtggtcgt taggagaagc tcgagatatt cttggaagta aagttgtgat
    29761 agataatcat gcaagaattt acagtccgaa gcatgaagaa ccgcgtttca aaaactctca
    29821 ttccccatat aagagcccga tgtagcttaa gaaaaagaca cgcccacctt gtgcgtctac
    29881 ttctggcaca gagcggactc tcagattagg ctttactctg tgccatagat atgtaagctc
    29941 acaccagagc tcatacaact tattgcggca tttccggcca ttcaggattt gcaggatcca
    30001 cacgactgac cagaacactg tagcgttccc atgcctccag tcgactgcgc tcctcatctg
    30061 ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat cacggattca gcatctgcaa
    30121 gaagtctggc tttccgattt tctgcctgct gctgcaattc ctctgccgta taaattcgtt
    30181 taatcactgt acagtcctta aacatccagt tccctgaaat gtccgcccgt cggttagcag
    30241 taatatccgg cacttcaaca acacttaatc catccggtct gatagctgtc acatcctttt
    30301 ccacatagcg gataatatta tctttgtcgt acgctatttt tatcgtgtca tcagcaaaat
    30361 acttttgttc ttcgtaccag ttcttaccat cttctgaaaa aaaccagaca acatcaaagt
    30421 cctttgtcaa ttgatattgt tcaaccgttt ttggattacc tgccgttata tttatcaaat
    30481 gctgcataaa ttatacctgt gccacgttat accaagtccc gttaatgtat ttctgcaccg
    30541 gtctgtaata tacgccacca atgttatcgg cagagtttga gccggtatcc tgaacaataa
    30601 tgccggaata tacacacccg gacggtgcct gatgtgtcca tgtcatacca ttgttcgcag
    30661 gtttgtatgt ggcagcacca ccaagccgga tatcccggac atagcgtgaa tcaaagttgc
    30721 catagttaga tggtgatacc tgcccgttaa cagcaaaagt gatgctgtta tctgtatttc
    30781 tctgactgta aaaatgccag cctgcatcat cacctaattc agccaccaca ggacgacttg
    30841 agtttcccca caaattgaat gcggcttcct tcgtggatgt attgctgctg ctgaccgtga
    30901 actttttccc gctaccggca cgtactttgg tacttgagac aatatcacct gtaacactca
    30961 ggccatgccc cattgacact ccgccattgg cgttattgat agtcagcggt cttaagccgt
    31021 tccatgtccc aaatttatcg ccagaggccg tcagcattaa atatgtgctg ccgccatcat
    31081 tcctgataaa gaatccataa ttgccataag caatgcgcag accattagca ctgagtgatg
    31141 taatttcacc tcttgaacgg agaccataag cggagctgag tgataattct tcctgagcgt
    31201 cattgtttcc tgtcgcccag cgaattaccc cgccctgaac tgtttcatgc cagatagtat
    31261 ctccttctcc accacgaaac tttctgagat attttttgcc gcctctggtg cctgaacata
    31321 aggccgtaga catataggca ttctggcttc cgccatcctg attaatcgtt ccggtcattg
    31381 agtcgccctg acgattccag tcacgacgcc agccggggga gtagccgtcc ccatgattta
    31441 tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga tgttgttggc gtggtcactc
    31501 ggatagtgat tgcagattta gttcccatga cctcgacgac acaaccagcc aggtggatat
    31561 caccacatcc ggtatccgta atgattttgt tatttgcata tgaccaggag cctttgcaca
    31621 tccagtacgg atgattaaat gcaccacggg aatccagcca ttcaataaac tgagcggttg
    31681 tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc acgacaggca ccgatatttt
    31741 tcgtgaaggt atcctttccc ggaatatccg caccgttctg atctttctga agacgttttt
    31801 cagcattgtc ataggcagac ttcactgctt ttggcgttgc ggccagcgtt tcagaatcgc
    31861 tgttgatggc actacttagc tggacaagac cttttcgcgc tgtggtggca tcctgtgcag
    31921 tgtatttccc gttagcaagg tcatacgctg tcttaaccgc ctttggtgtt gccgcaagcg
    31981 tttcagaatc gctgttggtg gcgctactga gctggacaag accttttcgc gctgtagtgg
    32041 cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc tgtcttaacc gcctttggcg
    32101 ttgccgcaag cgtttcagac gtgctgttgg tggcactact gagctggaca agaccttttc
    32161 gagctgtggt agcgtcctgt gcggtatatt tcccgttagc aaggtcatag gcggccttta
    32221 ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt ggtcgcactg cttaactgag
    32281 taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg tgactgttcg tgctctgcaa
    32341 ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt ggtgtcaatg gtcagctcca
    32401 ccgaggccac actgctgacg atgatgacca tgcgacaggt ctgcgaacgc cctgagcctt
    32461 cggcaagagc tggcttataa ctttcggcca tgttcgccac ggcaattaac gttcccgcat
    32521 catcgtacag gccaagctca cgcatccaga aaccgcccac ctccggcgga ataaccagct
    32581 ctgcgataat ataattactg tttcgtttgt cctggctgat tttgttcagc gtatgtcgcc
    32641 agacttcgtg gataagcccg gtctgtccgg catccgggac aggcaattta ccaccgccat
    32701 ccccgacggc catcgtggta atgttgacct tccgccctcc cggtgcggtt gccgctgcca
    32761 gctttgctgc accggcagtg gtgataacgg ttctgaattt tgtgctcatt attcctcact
    32821 tatccggggt aaaccgtaat tacatcgccg tcgtaagcca caccaccggc gaacaggtag
    32881 ccgggaatgt cccgggtaat gttcaggcca ataagatgac ggcttgcagg tttggcatca
    32941 gcaataagcc gttccatttc ctgatacatt gcctctgtga tgccgctttc cagtacacca
    33001 atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc accactccgt cacgttgatg
    33061 agatagccga gcggctccac cacacgccgg attgcgccga tagtgccttt atgacagtgg
    33121 atgaaatagg catcgcggat aacggcgcgt tttgtcgctt ccggccactt ttcatcccac
    33181 ctgtcgaccg aaaacgccca cgccagccac ggcagcagat ttgccggaca ggtatccggg
    33241 ttccacagtt cacgaatcct gaccggcgtt ttttcaattt ccgcacaggc ttttgcggcg
    33301 gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg aatcactcat ccgagcctcc
    33361 ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta ctgttgagca cgatgtcggc
    33421 cagcggtgca gtcagttcga cacgctgcac gccttccaca tgcaaagcgg cataaatggc
    33481 agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg atgtacgctt ccagtttttt
    33541 cacggcagca gcgcgtatgg gttcgctttc gggaccaggg taaaggtaaa gcgtggcgtt
    33601 tatctggtat tcaacgatgg cggcagactg cacggtcacg cggtcggcca ccggcctgac
    33661 gtcctcacca ttaagggcgt tacgcaccac cgccagcagg tcttcggatg cgacaccgtt
    33721 attttcacgt gacagcacag agatggtgac gcaggccgga gacggactgg ttacagaaat
    33781 atccgcgaca cgcccgtcgg cactgcgacc atgatactga taggccccca ccgacccggc
    33841 gacgcttaaa ccttcaaacg cctgctgaat acgcagacga taatccgtgt cagattccat
    33901 cactgccggt gtcggcggga gggtcgaatc atctgccggg gtgataatca ggcgcgtggt
    33961 gttgtaattg gcaccaatca catcaaggtc attaccggcg gcacaggcca gcattaccgc
    34021 ccgtgcggcc tcattcacac gctgacgcca gataagctca cgataagcat tttcctccag
    34081 cagtttgacg agaggctcgg attccagcgt cagggtacgg gcgaccgcct cctgctggtc
    34141 ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca agaatggttt caaagtccag
    34201 ctcctcgacc acatccggtg cgggtagctg gttcaggtcg ataatcggca tggtttcaac
    34261 tcacagggat ggttaacgaa agtggctggc cggtgtcgtt gtgctggccg gttaacgtga
    34321 ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga tgacagggtg acgcgggctt
    34381 cccatttcag cacggccatg taacaggcga ccttaatctg caactcaagc gccggggtct
    34441 gcggctggtc aatcattgac gccagcaacg agccgtaatc acgacgcatc acccgtgagc
    34501 cgaccggcgt gcgcaggata tcgccgatac tctggctgat atgctcaagg tcagagacag
    34561 tcaggccatc actgcgattc attccgagat aacgcgctgt catagaggac tcccggttgt
    34621 gccgccgctg tcgccggggt gtttatgggt atgcagtacc ttcccgtttg atgagagttc
    34681 accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc ttctgcactt ccagcgtgcc
    34741 agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc agagtgacgc gggttgatgc
    34801 tttcaccgtg accaccggca ccgtggcagt aacagaatca gaagccgtca cgctggccgt
    34861 tttaattccg cttaccgtga gtgcactggt ttcaggttca tactcaatca ccgccccgtc
    34921 agggaaacgg atatgcaggg catccgccga cgcagacggc gcggggttat caccggaata
    34981 aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct acggccagaa tcagcacctg
    35041 ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg gcgcgatggg tcagccactg
    35101 aagccagtcg gtgcacatgc cgccggtctg cacacggcag cgaccggcat taaggtcggt
    35161 ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg cgcgcgagtt cctgaatatt
    35221 tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat accggcggca atgtgacgat
    35281 gaggtgtcag gaatggcaca actaacggtc gaggtgagcc aggataatct cttcaatcat
    35341 ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc ggataatgaa ttttcttacc
    35401 gtctttccgg gtttcttccg acagaccgaa ctgatgcaca ctggctattt tcggtgactt
    35461 cccgccgtaa aactccattg atgcctgttc agggctggcg cggatatgca aaaaacgact
    35521 ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg gtcttttttc tggcgctctg
    35581 ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc tgtgccatca cccgacgctg
    35641 ctgactctga cgcagacgtt tcgccagttc ggcgctcagt cgccgacgcc ctgacggtga
    35701 cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc ttaaactcat tcatcccact
    35761 tactcaccag ttcgccattg atataaagct ccatcgggcg ggtgaccggc tccggcggcg
    35821 gaggttccgg gatattcttc acatgcagcg cgccgtccac ctcactgacc agcgtgcgct
    35881 cggtcagcat caggctgatg ctgatatcaa agctgctgtc attgttgatg tctgcataaa
    35941 acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat gtcgggctga ttttcccgca
    36001 gccacgccag caccggcacg atgagcaggt caaaatcacc ggtaaagtcg gtcacaatca
    36061 cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc cagtgtggag gcaatactcc
    36121 cgttatccac gaatatccgc agcatctcgg ggcttgtttt cagcaccgtg acggcatcag
    36181 tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt cgtcctgaca gtgtttaatc
    36241 atttttacct ggctggcaca gcgtgccagc gcgttctcaa gctgccggat atcagcactt
    36301 aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc aaaggctcac tttcgggcag
    36361 gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc tggtgcaacc ggcgcacagc
    36421 atcaggcagg tcagcaccgt accagcggcg aaaatcttcg ttttcattaa gtaacctcgt
    36481 gatggttttc tcgcgctgag cttcacgctt cgcggcgttc tccagttcct gacgcagtgc
    36541 cacctgcgcc agctcgtttt tgtctgccct ggtgagggca acatgaagct ggtttttcag
    36601 catggtgatg gtcgtctgct gttcactggc gacgttgttc gccctgtcca gcaaggcgcg
    36661 caggctggca tttttgtgtt tcaccagaaa cagaccggcc accgccagcg ataacaacac
    36721 aaccagcaca atcatcagtt ttgacatggt tcccgcccct caagacgctg acggcaggcc
    36781 gtacgtatca gccggaaaaa cagcgatgcc acgagataaa tcagtgcggt aaaaatccac
    36841 ccggcagcga ccagcgagat aaacgtcgcc accatcacca tcagagccgc tgcccgtctg
    36901 cgccacggca ccggctgcaa aaacagcgac gtgacaatct tcacggccag cgattccggc
    36961 ggcagctccc gcccgtagcg ttccagcaca tactccgcgg catacacgcc gacaccaccg
    37021 gcaatcacac agataaccgt cgccagaatc gcccatgcag cgacaaaact gacggccacg
    37081 ctctgcgggt aaatcaggga cagtgccagc atcagcgcca gcgacacgtt cagcatcagt
    37141 gaaagggata atttcttcat ggtgtttact ccgtttaagc cggtacgccg ccagcggtgc
    37201 gccagacggt gaccagtttt tccagtgaat gctcacgctg accgtaaccg gcacccggca
    37261 gggacgccca gatattgcga cagcgtgaaa tggcgcgctc aatgcgtccc gcccggatgt
    37321 catccagtgc accgcgttcg cggatcaact gaatggcgag cctgtcctgt gacaacggac
    37381 tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca gaacaggtaa agctgctgat
    37441 agcgaccgga ggccgtggat ttttcaccgc gacggttaaa caccttcgcc ggtcggccat
    37501 gtgcgaacgg gtggtcactg tagtcggtga agatttccgg cttcccgtcc agtccggtga
    37561 ctatcacgtc atagccccgg tttttcgtca gcggatgatt cgccgtccct tcggacacgg
    37621 ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct aattaccggc attacggttt
    37681 ccccctgccc ttaaagcggc gctgaatggc aatctcaatc acctgataac cggcgatacc
    37741 cagcatggag ccgatgccgc acaccgcagg cagtgacagg tcaggaaact gcaccagaac
    37801 aacaccggca accatcgaga caaaaccacc gagcaacatg cgcccgataa acagacgcgg
    37861 agtgatgggt tcaccaccgg caagcacctt gccgacaaca atcagcaccc caatcatgaa
    37921 aagcgacagg acgctttttt cttctgctgt catgcgttac tcccacagat tgacagtttc
    37981 agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg gtgccgtgtg gcagcaccgc
    38041 ccccagttca gccagtcccg gatttgcggc gagcacggcc tcgaccacgc cctcagtgcg
    38101 cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc tgtagcgcaa aggtcttcat
    38161 cagatttgac tcacgatgca gcgcggcttg tcctggatac gcgccaccgc ccagcgcata
    38221 tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg ccttcttgtc gcccttggca
    38281 ctggcatcca cgccgcggta acgctcataa agcgacgcgg tcgccatcgc acacacagcg
    38341 cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt cgtccgccgg gacgtcagcc
    38401 agacgcgaaa aaccggcagc aattttctgt tcgcggtact cgtacagctc cgcattcgtc
    38461 tccgccatgc ctgacttgat ggcctcacgc agacgggcgg gggcgacggt ctgctcaagg
    38521 cgcatacgtt cccggacgcg cttcgggtca atatcgggaa aaaagaacgt gtttttaatc
    38581 accggctcgt cgcctgccgg ttgcgggata accaccgtac cctcaccgga cacgggagcc
    38641 tcctttcgcg gaataatcag cgtcatcatg actacctctg aaaagtcggg cggtggacgc
    38701 cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg ccgccctggc gcggggcgca
    38761 ttcggttgtt aactggcttt ctttttcggg cgtccacgtt ttgccggtgt cacgctccgg
    38821 gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct ccggcttcgg tttcagctcc
    38881 cgctccagtc gttcaatctc ttttttgacg cctgcctgac agtcgagctg tgtcgcacgt
    38941 tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac gcagaaacag accggtgatt
    39001 ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg tggcggtcag ttcgagggtg
    39061 tccgtcagca ggcggggatc cacagactca ccggcagcgt gagcgcgcat ggccgcaagc
    39121 gccacctcct cggtgaacat gtacggcggg gtgcggcggt gtttacccgg catggtcaga
    39181 ccgtacttca gggcataacg ggcaatctcc agcgcaccgg caatatcgcc ggtatccaga
    39241 cgccacagca tgaccgtcat cagaatgtca tcctgtgcac ctttgccctg ctccagcacg
    39301 ccgttcaccc acggcaacca gaacggcagc agttcgcgtt ttttcgcggc cttcagctct
    39361 tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca gcttgaccag catctgctca
    39421 tagacagttg catgtcgcag cggggcggct tcccgctgcg cggtcatcgc tgccgagacc
    39481 cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg ctcccggctc tgcggtcgct
    39541 ttagccggtg tggagaaatc accgacctta attttttcca ccagacaacc ggcggcgtag
    39601 tcctccacca cgtaatcaat gttcattgac tcgtagttct ccacgcggtc gagtttcggg
    39661 ttttccacaa tcacgcggcg atggctgtca tccatgtagt agatggacag gttttccagc
    39721 ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc gtaccgccgg caggttaccg
    39781 atgcgtttct ggctgatgat gacgtcagcg gccagcatct cgctgttatc ctgctccttg
    39841 ttgacgatag ggaaatactt gtccgccagt agctgacgcc ccacaatcac cacaaggtca
    39901 gggtcttcct gataccaagg ttcaatcagg ttgttggtcg catccatcac cagtgcatcg
    39961 aggcttacat aatcaccgcc cttacccacg cggataacct cagaggtggt gcggccttcc
    40021 tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat tcggtatttc tgcagccagc
    40081 cgaccgccac atcctgcagc atcggattac tgttgcggtc agaggtttcg gcacgcttca
    40141 cgccgttaaa accggccatg atgaaatcaa gggactggcg tttgataatg gcgttacgga
    40201 tacggagctg gaaatcctga taacgcgccc acaggtcaag cgttttgtag cggatataaa
    40261 aatcgaagtt aacctggtcg cattcgtact tgtttgacgc cagcttcgag aagtccttcg
    40321 gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc gatggagccg gtgacaccga
    40381 tgccaatttt ttcccctttc atttcactga ccggcacaat gttgatgcgg gtcagaaagt
    40441 cagaggactc ctgcatggtg ttcatcaggg tctgggtgac cgacggttca acggtgaatt
    40501 ttttcgacac atcaccggcg tcgatgccgt tcagctcggc aacacgggac aggtaagcat
    40561 taaatttaaa gcgggtttcc tggcgcatag tttttcctga aattaagggt taatcgtgaa
    40621 ggttttcccg gactgactga cgccggtcag cagttcgtca tcagggcgtc accgccacca
    40681 ccggtggcct tgctgcggcg ctgctgggtc agactttcgg tgtggtcgag actgtttttc
    40741 aggcgggtga atgcctggct ggtttcatcc gccctgtcag tcacctcctg cttaagtgca
    40801 gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg cgctcagttt ttcctgcaca
    40861 tgttcagcaa cagcggtcac cgcttcatgc acgtcattca gacgggcatc atcgctggcc
    40921 tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg cggtgaacac ggtttcaggc
    40981 aggtcttcaa attccagctc aacaggcgtt gccactgaaa tcaggttttc agggcttaat
    41041 ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga attccaggta ttccgtgccg
    41101 aggcttgccg ggtcatcggt gacggccaga cccaccagat aacatttgcc ggtgttggca
    41161 aagttcggct gaatttccat tgaggtgtag accttctgcg cggccttgtt catcgcgata
    41221 aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt tgcctttcag cgccgaatca
    41281 tcgtcaatct tttcggcctt cagttcggcc acatcgccat aacgcttaaa aataccgtca
    41341 ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac catagacacg cgggtcaaag
    41401 gtttcggcca tttcctgaat atcctgcgca ctgatgacac gcccgtcaca ggtgtcaccc
    41461 tcaacgccga tacgaaagaa ttttgagact ttttttgcca ttgtcaggag tcctgaatag
    41521 tgattagagg agtcacatgt cggcatcagt ttcccgacga tgcgcatcct ccgccatcag
    41581 tcccggatgg tttatcactg acacaacagc acattagcga atcgcggggc gcgactcagt
    41641 agccttgccg tgtattcatc acggcgaggt attcatgacc atcaccacag acaccactct
    41701 tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg caggggtttt ccgtgccgca
    41761 gattgccgcc atgttgcaga tgaaacgccc gacggtgcag agctggaaac agcgcgacgg
    41821 ctgggacagt gttgccccca tcagccgtgt cgaaatgagt ctggaagcgc ggctgaccca
    41881 gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag gaaattgacc tgctcggacg
    41941 ccagattgaa cgactggcac gggtaaaccg ctacagccag accggcaacg aggcagacct
    42001 taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc aaaccgaaaa agaatttttt
    42061 cagtgacgag gccatcgaaa agctggagca gattttcttt gagcagtctt tcgactatca
    42121 gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc gatatcctga aatcccgcca
    42181 gattggcgcg acgttttatt tttcccgcga ggcgctgctg cgcgccctga aaaccggcca
    42241 taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat gtgttccgtg aatacatcat
    42301 cgcctttgct cgtctggttg acgttgacct gaccggtgac ccgattgtcc tgggcaataa
    42361 cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc gcacagagcc ataacggcga
    42421 cctgtacgtc gacgagattt tctggatccc gaattttcag gtactgcgta aggtggcatc
    42481 aggtatggcc tcacagagtc acctgcgctc gacctatttc tccaccccgt ccacgctggc
    42541 gcacgacgcc tacccgttct ggtcgggtga actgttcaac cggggacgcg ccagcgccgc
    42601 cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc ggtggtcttc tctgtgcgga
    42661 cggccagtgg cggcagattg tcaccattga ggacgccctg aaaggcggct gcacattgtt
    42721 cgacattgag cagctcaaac gcgaaaacag cgccgacgat tttaaaaacc tgttcatgtg
    42781 tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag gagctgcaac gctgcatggt
    42841 cgacacgctg gaagaatggg aagactacgc gccgtttgcc gcaaatccgt tcggctcccg
    42901 cccggtatgg attggttacg acccgtcaca ccgtggcgac agcgccggat gcgtggtgct
    42961 ggcaccgccg gtggtggccg gtggcaaatt cagaatactt gagcgtcacc agtggaaagg
    43021 catggacttt gccacccagg cggagtccat ccgcaaactc accgaaaaat acaacgtcga
    43081 atacatcggt attgatgcca ccggcctcgg tgtcggcgtg ttccagctcg tgcgctcgtt
    43141 ctatcccgcc gcgcgcgata tccgctacac gccggaaatg aaaaccgcaa tggtgctcaa
    43201 ggccaaagac gtcatccgcc gtggctgtct ggaatatgac gtcagcgcca ccgacatcac
    43261 cagctcgttc atggctatcc gcaagaccat gaccagcagc ggacgcagtg ccacctatga
    43321 ggccagccgc agcgaggaag ccagccacgc cgacctcgcc tgggcaacca tgcacgccct
    43381 gttaaatgag ccactcaccg ccggtatcag cactccgctg acatccacca ttctggagtt
    43441 ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg cggcaaaaac aatcaccgcc
    43501 agcgccccga aaatggaagc attcaccttt ggtgagccgg tgccggtact cgaccgccgt
    43561 gacattctgg attacgtcga gtgcatcagt aacggcagat ggtatgagcc gccggtcagc
    43621 tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc acagctcacc gatttacgtc
    43681 aaacgtaata ttctggcttc aacgtttatc ccgcacccgt ggctttccca gcaggatttc
    43741 agccgttttg tgctggattt tctggtgttc ggtaatgcgt ttctggaaaa gcgttacagc
    43801 accaccggta aggtcatcag actggaaacc tcaccggcca aatatacccg tcgtggcgtg
    43861 gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc cgacagcctt cgcgcccggc
    43921 tccgtgtttc acctgctgga gcctgatatt aatcaggagc tgtacggcct gccggaatat
    43981 ctcagcgccc ttaactctgc atggctgaat gagtcggcca cgctgttccg ccgcaagtat
    44041 tacgaaaacg gcgcacatgc cggatacatc atgtacgtca ccgatgccgt gcaggatcgc
    44101 aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga aaggccgcaa taactttaaa
    44161 aatctgtttc tctatgcccc acaggggaaa gccgacggca ttaaaattat cccgctcagt
    44221 gaagtggcaa cgaaggacga tttttttaat atcaaaaaag ccagcgccgc tgacctgctg
    44281 gacgcgcacc gcatcccctt tcagttgatg gggggcaagc cggagaacgt cgggtcgctg
    44341 ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg agcttatccc gttacaggac
    44401 aggatccgcg agataaacgg ctggctcggt caggaggtca tccgctttaa aaactactca
    44461 ctggacactg acaacggctg aacatcgccg cctgcgggcg gcttttttac accccgccat
    44521 cacgccctca cacgctcatc accgcacaaa aaatcccgca gacacaccaa cgcccaacgg
    44581 gcagactaag cgccgtcacg acgcgctgag acgctggaaa ataaaatcag caccaccgcc
    44641 agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg gggcggtttt aatgcagttg
    44701 catgaacact ccaagagcac gccagcgcta gtgataacta acaataaaag cgacttctga
    44761 cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc tatgcggtat gctatccgct
    44821 tcaattttta acacttgtta agggaactag acatggacat tattgattta aaatctcaac
    44881 tagaaaatga agataatgcc aagaaaagag cagagttgct tgctcagcta gaggtgataa
    44941 agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga tgtggatttt gagaccaaag
    45001 aatttaccgt tgagttatta gttaataagt atcataacgg attagaggat gatactaacg
    45061 aactctttgt tcctgattat caaagggatt ttgtttggag tgaaaaaaga caatcaaaat
    45121 tgatagagtc tttgattttg ggttttccta taccatatat atttatagca gatgttctct
    45181 ccgaagatcc cgatttagat gggcgaattg agattgttga tggctcacaa cgagttagga
    45241 ctatacacgc ttttatccaa aatgaattag ttttacaaga cctgaaatct ttagacgtcc
    45301 ttaatggttt caaattccaa gatttaccat tatcacgtca acgtcgtttc atgagaattc
    45361 ctgtccgcgt tatagaattg agttctaaat gcaatgaaga aacccgtaga gatctttttg
    45421 agcgtattaa ctctggcagc gatatattga aagatatgga agtccgtaaa ggatcagaac
    45481 ttggttcaac aaatttatat acacaggtta ttaagccatg ctcaacaata cctaaattta
    45541 aagaattagc acctttatct cagactaaag aaaaacgaga tgaacggtta gaatttacat
    45601 taagattctt tgcctacgta gataagtaca agaactttga tcattccgta cgggattttc
    45661 taaatgacta tatgtcagaa aatcgagata tagacgaaac taaacaaaac tcaatgaaac
    45721 aagagtttgc taatgttcta tcttttgttg aaaactattt cccagcagga ttcaagaaaa
    45781 ctattactgc aaaatctaca cctcgagtta gattcgaatc tctcgctgtt ggcgcagcac
    45841 ttgcattacg tgaagtccct gaccttgttc ccaagaactt agattggcta agctcagaag
    45901 aattcaaacg attgaccact tccgacggag ctaatagtcg cgtgaaggta attgaacgaa
    45961 ttgaatatgt tcgggataaa ttattagagg attaacatgg atcgttttcg tgaggatttt
    46021 gacgaaaggt caggagagat cttggcctat ttggatcttt tgaaatttat tgagtatgcc
    46081 ggtgcagaac tgatatcagc tgatgataaa gaacataagt tctccattac tgctcaatca
    46141 cgaaaaactc taaagggagc agtatatata ctgttatata acctcattga atcaacaatg
    46201 agagaagcta tctgccttat tcatga
//
LOCUS       DAZOYS010000010        45707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOYS010000010 DAZOYS010000000
VERSION     DAZOYS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.010"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(83..1942)
                     /gene="gss"
                     /locus_tag="ACHUN9_000465"
     CDS             complement(83..1942)
                     /gene="gss"
                     /locus_tag="ACHUN9_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0693977.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2147..3013
                     /gene="yghU"
                     /locus_tag="ACHUN9_000466"
     CDS             2147..3013
                     /gene="yghU"
                     /locus_tag="ACHUN9_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0693978.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3042..3123)
                     /locus_tag="ACHUN9_000467"
                     /pseudo
     CDS             complement(3042..3123)
                     /locus_tag="ACHUN9_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3136..3384)
                     /gene="hybG"
                     /locus_tag="ACHUN9_000468"
     CDS             complement(3136..3384)
                     /gene="hybG"
                     /locus_tag="ACHUN9_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0693979.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3397..3738)
                     /gene="hypA"
                     /locus_tag="ACHUN9_000469"
     CDS             complement(3397..3738)
                     /gene="hypA"
                     /locus_tag="ACHUN9_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0693980.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3731..4219)
                     /gene="hybE"
                     /locus_tag="ACHUN9_000470"
     CDS             complement(3731..4219)
                     /gene="hybE"
                     /locus_tag="ACHUN9_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0693981.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4212..4706)
                     /locus_tag="ACHUN9_000471"
     CDS             complement(4212..4706)
                     /locus_tag="ACHUN9_000471"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0693982.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4706..6409)
                     /gene="hybC"
                     /locus_tag="ACHUN9_000472"
     CDS             complement(4706..6409)
                     /gene="hybC"
                     /locus_tag="ACHUN9_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0693983.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6406..7584)
                     /gene="hybB"
                     /locus_tag="ACHUN9_000473"
     CDS             complement(6406..7584)
                     /gene="hybB"
                     /locus_tag="ACHUN9_000473"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0693984.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7574..8560)
                     /gene="hybA"
                     /locus_tag="ACHUN9_000474"
     CDS             complement(7574..8560)
                     /gene="hybA"
                     /locus_tag="ACHUN9_000474"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0693985.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8563..9681)
                     /gene="hybO"
                     /locus_tag="ACHUN9_000475"
     CDS             complement(8563..9681)
                     /gene="hybO"
                     /locus_tag="ACHUN9_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0693986.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9870..10157)
                     /gene="yghW"
                     /locus_tag="ACHUN9_000476"
     CDS             complement(9870..10157)
                     /gene="yghW"
                     /locus_tag="ACHUN9_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0693987.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10276..11163)
                     /gene="yghX"
                     /locus_tag="ACHUN9_000477"
     CDS             complement(10276..11163)
                     /gene="yghX"
                     /locus_tag="ACHUN9_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0693988.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11320..12360
                     /gene="gpr"
                     /locus_tag="ACHUN9_000478"
     CDS             11320..12360
                     /gene="gpr"
                     /locus_tag="ACHUN9_000478"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0693989.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12400..12894)
                     /locus_tag="ACHUN9_000479"
     CDS             complement(12400..12894)
                     /locus_tag="ACHUN9_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0693990.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13085..13969
                     /gene="yghA"
                     /locus_tag="ACHUN9_000480"
     CDS             13085..13969
                     /gene="yghA"
                     /locus_tag="ACHUN9_000480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0693991.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14129..14554)
                     /gene="exbD"
                     /locus_tag="ACHUN9_000481"
     CDS             complement(14129..14554)
                     /gene="exbD"
                     /locus_tag="ACHUN9_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0693992.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14561..15295)
                     /gene="exbB"
                     /locus_tag="ACHUN9_000482"
     CDS             complement(14561..15295)
                     /gene="exbB"
                     /locus_tag="ACHUN9_000482"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0693993.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15547..16734
                     /gene="metC"
                     /locus_tag="ACHUN9_000483"
     CDS             15547..16734
                     /gene="metC"
                     /locus_tag="ACHUN9_000483"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0693994.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16874..17533
                     /gene="yghB"
                     /locus_tag="ACHUN9_000484"
     CDS             16874..17533
                     /gene="yghB"
                     /locus_tag="ACHUN9_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0693995.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17573..18472)
                     /gene="yqhC"
                     /locus_tag="ACHUN9_000485"
     CDS             complement(17573..18472)
                     /gene="yqhC"
                     /locus_tag="ACHUN9_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0693996.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18666..19829
                     /gene="yqhD"
                     /locus_tag="ACHUN9_000486"
     CDS             18666..19829
                     /gene="yqhD"
                     /locus_tag="ACHUN9_000486"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0693997.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19934..20761
                     /gene="dkgA"
                     /locus_tag="ACHUN9_000487"
     CDS             19934..20761
                     /gene="dkgA"
                     /locus_tag="ACHUN9_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0693998.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20961..21887
                     /locus_tag="ACHUN9_000488"
     CDS             20961..21887
                     /locus_tag="ACHUN9_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0693999.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21938..22195
                     /gene="yqhH"
                     /locus_tag="ACHUN9_000489"
     CDS             21938..22195
                     /gene="yqhH"
                     /locus_tag="ACHUN9_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0694000.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22238..24457)
                     /locus_tag="ACHUN9_000490"
     CDS             complement(22238..24457)
                     /locus_tag="ACHUN9_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0694001.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24568..25980)
                     /gene="ftsP"
                     /locus_tag="ACHUN9_000491"
     CDS             complement(24568..25980)
                     /gene="ftsP"
                     /locus_tag="ACHUN9_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0694002.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26055..26792)
                     /gene="plsC"
                     /locus_tag="ACHUN9_000492"
                     /gene_synonym="parF"
     CDS             complement(26055..26792)
                     /gene="plsC"
                     /locus_tag="ACHUN9_000492"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0694003.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27025..29283)
                     /gene="parC"
                     /locus_tag="ACHUN9_000493"
     CDS             complement(27025..29283)
                     /gene="parC"
                     /locus_tag="ACHUN9_000493"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0694004.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29829..30311)
                     /locus_tag="ACHUN9_000494"
     CDS             complement(29829..30311)
                     /locus_tag="ACHUN9_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0694005.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30364..30756)
                     /gene="ygiW"
                     /locus_tag="ACHUN9_000495"
     CDS             complement(30364..30756)
                     /gene="ygiW"
                     /locus_tag="ACHUN9_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0694006.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30908..31567
                     /gene="qseB"
                     /locus_tag="ACHUN9_000496"
     CDS             30908..31567
                     /gene="qseB"
                     /locus_tag="ACHUN9_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0694007.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31564..32913
                     /gene="qseC"
                     /locus_tag="ACHUN9_000497"
     CDS             31564..32913
                     /gene="qseC"
                     /locus_tag="ACHUN9_000497"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0694008.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            33023..33604
                     /gene="mdaB"
                     /locus_tag="ACHUN9_000498"
     CDS             33023..33604
                     /gene="mdaB"
                     /locus_tag="ACHUN9_000498"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0694009.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33635..33949
                     /locus_tag="ACHUN9_000499"
     CDS             33635..33949
                     /locus_tag="ACHUN9_000499"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0694010.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33997..35889)
                     /gene="parE"
                     /locus_tag="ACHUN9_000500"
     CDS             complement(33997..35889)
                     /gene="parE"
                     /locus_tag="ACHUN9_000500"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0694011.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35918..36499)
                     /gene="yqiA"
                     /locus_tag="ACHUN9_000501"
     CDS             complement(35918..36499)
                     /gene="yqiA"
                     /locus_tag="ACHUN9_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0694012.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36499..37326)
                     /gene="cpdA"
                     /locus_tag="ACHUN9_000502"
     CDS             complement(36499..37326)
                     /gene="cpdA"
                     /locus_tag="ACHUN9_000502"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0694013.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37351..37773)
                     /locus_tag="ACHUN9_000503"
     CDS             complement(37351..37773)
                     /locus_tag="ACHUN9_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0694014.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37774..38403)
                     /gene="nudF"
                     /locus_tag="ACHUN9_000504"
     CDS             complement(37774..38403)
                     /gene="nudF"
                     /locus_tag="ACHUN9_000504"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0694015.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38608..40089
                     /gene="tolC"
                     /locus_tag="ACHUN9_000505"
     CDS             38608..40089
                     /gene="tolC"
                     /locus_tag="ACHUN9_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0694016.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40237..40908
                     /locus_tag="ACHUN9_000506"
     CDS             40237..40908
                     /locus_tag="ACHUN9_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0694017.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40914..42074
                     /locus_tag="ACHUN9_000507"
     CDS             40914..42074
                     /locus_tag="ACHUN9_000507"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0694018.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42112..42900)
                     /gene="ygiD"
                     /locus_tag="ACHUN9_000508"
     CDS             complement(42112..42900)
                     /gene="ygiD"
                     /locus_tag="ACHUN9_000508"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0694019.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43043..43816
                     /gene="zupT"
                     /locus_tag="ACHUN9_000509"
     CDS             43043..43816
                     /gene="zupT"
                     /locus_tag="ACHUN9_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0694020.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43874..44044)
                     /gene="yqiD"
                     /locus_tag="ACHUN9_000510"
     CDS             complement(43874..44044)
                     /gene="yqiD"
                     /locus_tag="ACHUN9_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0694021.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44305..44958)
                     /gene="ribB"
                     /locus_tag="ACHUN9_000511"
     CDS             complement(44305..44958)
                     /gene="ribB"
                     /locus_tag="ACHUN9_000511"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0694022.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45062..45210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45332..45622
                     /gene="ubiK"
                     /locus_tag="ACHUN9_000512"
     CDS             45332..45622
                     /gene="ubiK"
                     /locus_tag="ACHUN9_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0694023.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11281 a        12261 c        11470 g        10695 t
ORIGIN      
        1 agaacatata aatattgaat attttgatta atccccgtac tgattattct tcataatcag
       61 tacggggttg ctacaacatg tattactttt tcaccacaat taacggttca atatcactct
      121 cttttttgat gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag ttgccgccca
      181 cggtgaaggt acatacctga atgtatttac cgtccacttt cggcaaacac cacagttgct
      241 gatagatgtt tttctgctcg gcaaatttac cgctggtttt gtccagcacc tcttcatgat
      301 ggctgaccag gtcgatattg ctaccacagc gaccggcaat cggtttcact gcgtaacctg
      361 ttttcaccaa ttcatcatta acagtgaaat cggtatccag caggtaacga tggtgcggga
      421 acagcgacca gaggatcggc agaatcgctt tgttgccggg gatcaccgtc cacagcggct
      481 caaagactag cacttccggg cgcagcaata cgtcgataag acgcacttcg ttttgcggat
      541 gaccggtacg gattggcacc gcagcaaact cacggtcgct aacttcacga atctgatcaa
      601 acgcggtttc ccacgcccag gttttccaca cgcagttaac cagtcgccct tccccatcaa
      661 tcagttgccc ggcagcatcc cagcccagtt catccaatcc acgcaagata cgcgtttcaa
      721 agcccgcctg gtgcagcgcc tgctccataa actgcgcgtg atagttttcc tcgatatctt
      781 tgtcctgcat gatatggaca aacggacgtg cacgactgtg tttccaggca ccagccaatt
      841 cgttaatcag cccttccgcc ggattgaagc cgttgccttt atagccctgc tccgcccaac
      901 gttcgaggat caagcccgct tcggtatgac aggaggcgga gtcggcgttg tactcgtaaa
      961 ccttcaggcc acgctcatcc atgcagaaat ccatacgacc agtgatcata tggtgacggc
     1021 gacgctgcca ggagagacgc aaacgtggcc agaggatttt cgggatgtcg aacagcgcca
     1081 gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag atacatcagg tgcagctcgt
     1141 tggtggcttt aattagctcc tgctcggcac tctcggtaat ggtgtagtaa tgataaggat
     1201 cctgattgat cacctgaccg ttggcctgca cataggcgtt ttgcagcgga tctttttcat
     1261 ccagccattt accgtcaaac tggcctttgt tttccaggcg cgctccgctg attttcagca
     1321 gctcgcctgc aatttccggc tgcggtaagc tgtattcagt atcttccgtc tggatcatcc
     1381 agcccagaat ggtggtgtca tcaaaagtgt ctttcagggt atagcagccg ttttcgacca
     1441 ccatctccag ctcacgcgtc cactgttgcc cttgcggcaa cggggaatga atcacgttct
     1501 gttccgcaat acggactttg ttgccatgca attgggtaat gatggcgaca tggccagtgt
     1561 ctttaaattc accgccttta tcccagataa gaagcgcacc cgcgaccggc gcacgcggcg
     1621 agccgttagg aaatgcctgc aatggcagga tgttgtcatt aaccacttct cgcaggaagc
     1681 gcagcgagaa aatctcccac gccataccca cgtcagtaaa gaccacaccg taattcagaa
     1741 agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg gcccatatat tcgtcgtcga
     1801 tatagctacg gaatacggcg tcatcttcgt attcctgcgg atcgagagaa ctgtaatctg
     1861 aagagtagat tgctacccca cctggggcgt agcccaataa tgtcccgaac ggggcatcct
     1921 ggctggtcgt tcctttgctc atcactttac ctttaacaat acaacctaag cagttggcgt
     1981 aattgttgtg ctctgattac ctgctcagcg acattaaccg gacagaggtc agactaatca
     2041 tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg tacagcaagt
     2101 gacctgctac actgcttcaa cactaccact cagaaggcaa ctcactatga cagacaatac
     2161 ttatcagccc gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt tcgccaatat
     2221 caatcgcccg gtttctggtc cgacgcatga aaaaacgctg cccgttggca aacacccatt
     2281 gcaactttat tcgctgggaa cgccgaacgg tcagaaagta acgattatgc ttgaggagct
     2341 gctggcgctg ggcgttactg gtgcagagta cgacgcctgg ctgattcgta ttggcgatgg
     2401 cgatcaattc tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc cggcgctgcg
     2461 cgatcatacg cataatccgc cgatccgcgt gtttgaatct ggttcgatcc tgctttatct
     2521 ggcggagaaa tttggctact tcctgccgca ggatttggca aagcgtactg aaacgatgaa
     2581 ctggctgttc tggttacagg gcgcggcacc gttcctcggc ggtggttttg gtcactttta
     2641 ccattacgcg ccgatgaaaa ttgagtacgc catcaaccgc tttaccatgg aagccaaacg
     2701 tctgctcgac gtgctggata agcaactggc gcagcataag tttgttgcgg gcgatgagta
     2761 caccattgcg gatatggcga tttggccgtg gtttggcaat gtggtgttag gcggcgtgta
     2821 tgatgccgca gagtttcttg atgcgggcag ttataagcat gtacaacgct gggcgaaaga
     2881 agtaggcgaa cgtccggcgg tgaaacgtgg gcgtattgtt aaccgcacca acggaccgct
     2941 gaatgagcag ttgcatgagc gccatgacgc cagtgatttc gagacgaata cggaagataa
     3001 gcgtcagggg taagggttgg tgttcgtcgc agcaagccat ccaggccgga taaggcgttc
     3061 acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg ctgccgcgtc ttatcaggcc
     3121 tacgaaagct aatcatcacg cgctggtaat gtcgtaatcc atttggcgca gtgcgtctaa
     3181 tgtggctttg gcttcatctt cgtcgatgat gctcatggca aatccgacgt gtaccagcac
     3241 ccactggccc agtagatcgg cagggttacc ttcacaaatc agggcgatat tcacgtcgcg
     3301 cttgatacca catacttcaa cctgcgcaag ctggtgaata tcttcaccga cagccagcac
     3361 ctggcctgga acgccaatac acattattaa ctccggttat tcaacttcaa tacttttgac
     3421 gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc
     3481 atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaagcct gggcgggttt
     3541 atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact
     3601 aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt
     3661 gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca
     3721 aagagacaac tcatgcattt tcgccactcc tgcgaccaaa cagcagggcg cgacgccctg
     3781 cgtgtggtac atccggattc gtgactggca gcgaaaggat cattcgtgcg cagtcatccg
     3841 tcaggcgttg gccctcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg
     3901 agagatattg cgaaacaccg tccagttcgc caacggtaaa ggtcatagtg ccatacggca
     3961 gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga
     4021 agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca
     4081 gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg
     4141 aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac
     4201 ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc
     4261 agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag
     4321 ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac
     4381 tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg cggagagatt
     4441 ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggcgtg
     4501 ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca
     4561 agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat
     4621 cgttgctcta acgcttcgac aatccgcaca ccgatggctt catcggtcag caaaatattg
     4681 ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt
     4741 cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga atggtacgca
     4801 ccacttccag cggtttattc ggatcagcaa ccggtgtacc caccagcgac tgctcgtaag
     4861 gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga acaactgcct
     4921 ggtagttact gataataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg
     4981 cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggt ttcacaaagg
     5041 tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattgattt tgcaggatat
     5101 cctgcaattc gcagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct
     5161 gcgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt
     5221 tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc agcggaccca
     5281 cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag
     5341 accaaccatc ataagccgga atggtggtac cttcccacgg tgcctgcggc gcttcgtctt
     5401 tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag
     5461 aagtgatcgg acgatacgag gacagatccg cattctcaat gtagccgccc gggaacagga
     5521 agctgccgtt tttactgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt
     5581 taccgcgcgt cagccattcc gggtagaacg cggcaataac tgcggtatca accttataaa
     5641 cctgttcaac aaagtcgctc agcttgtcga tgaaagactt gatgtacatc aggcgctcaa
     5701 ggttcagcac gcccaaaccg tcgaggttga tcgggttcgc gacaccacct accgccaggt
     5761 tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc
     5821 actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca
     5881 tcgccgggtg acccccgtag ccattggcga aaatacccaa ctgaccgctg gcaaccagat
     5941 ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc caggtcgaaa
     6001 cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca
     6061 cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag
     6121 ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt
     6181 ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca
     6241 caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca
     6301 tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta
     6361 aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc
     6421 ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag
     6481 tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca
     6541 ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accagcgaat aggtcagacg
     6601 ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatgc gggaatcatt
     6661 acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca
     6721 gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag
     6781 ctcgccaaag cgcagcacga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt
     6841 gacgaacaga ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagcgaacc
     6901 ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa
     6961 catttcatag ctctgccaca acggatgcac cttgtagccc gccgagatca tcagtgaccc
     7021 cattgaagac tggtgcatgg tcggcaacag cgcgccaagt gcaatgatga agaacataac
     7081 cttgttcaga cgcttgagag aaaccttcca gcccagacgt tcaaacagtg ccggagcaaa
     7141 ctccagtgcc atcacgccga tatagatggt catacagacc gccgtctcga acagtaccga
     7201 gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc
     7261 gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg
     7321 caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc
     7381 gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa
     7441 gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag
     7501 catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga ttttgccgcc
     7561 cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt
     7621 tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat
     7681 acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca
     7741 ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt
     7801 tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg tcgccagatt
     7861 tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg
     7921 cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta
     7981 cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc
     8041 acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg
     8101 cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt
     8161 tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt
     8221 gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgacccctg
     8281 tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg
     8341 accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga
     8401 cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa
     8461 ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg
     8521 ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc
     8581 agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc
     8641 aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc
     8701 tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg
     8761 atggatgcct ttatggaagc cgataccttc ttcgttacag ccatagcatg ggtgaccaat
     8821 cgctaccggc cacacgccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt
     8881 ttctggccct ttacagccga ggtggtacag gcaccagcct tcacggtggc cttcatcacc
     8941 gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat
     9001 cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta
     9061 agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat
     9121 attgataacg gttttgcctg gcagaacttc ttgcaggctg actgcgccag ttgggttaac
     9181 tccagctgcg gcaacaccgc cccacgcaga gcaggaaccg atagcgataa tcgctgctgc
     9241 gccttctgcc gctttgcgga tgtgatccac aatcggctca ccagcaacca tgcaataaat
     9301 accgttatct tttaatggga tggaaccatc cacaaccaac acatactgcc ctttgtactt
     9361 ctcaagtgcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc
     9421 gtgatactcc agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg
     9481 aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg
     9541 ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt
     9601 ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat
     9661 gagggtgtta tctccagtca tttatagtta ttccgttgcg aagacctggc atatattttg
     9721 cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt
     9781 atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt
     9841 aattaaagca agataatacg tatgtttgat caattttcga ggcgataacc ggccataaaa
     9901 aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc
     9961 agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc
    10021 ttttcgtaca tccggtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat
    10081 tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta
    10141 ccaaaatggt tattcatcgt tcacctcatt cgctatatat cgccatatat agcgatattt
    10201 taggcaaaga acgacttttg cgcacgctat tttttcatgc ttgtttaaga cgtaatccag
    10261 taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc
    10321 aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac
    10381 ccccggatag atatacgcct cataaacctt attattggct ttcaacgccg cctcgtaagc
    10441 aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc
    10501 aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag
    10561 ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat
    10621 acccactttg cctgccgctt gtggatagcg ttgcataaac tcaattgcgg caaagaaatc
    10681 attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat catcatttcc
    10741 cggataacct ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgc
    10801 cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac
    10861 cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc
    10921 gtgaccattt ggcgaaggat acatgatgta ctcagcaaca atctccgggt cggtaaactc
    10981 tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat
    11041 cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg
    11101 agcgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg
    11161 catcgttgca ctcctcagtt ggtgtttttt taagcaaagc ataagcacgt gtttttgccc
    11221 agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag
    11281 aaacattgtt tcgaaattga acggtggaaa ggagaggtca tggtctggtt agcgaatccc
    11341 gaacgttacg ggcagatgca ataccgctat tgcggaaaaa gcggtttacg cctgcccgcg
    11401 ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgcg
    11461 atcctgcgta aagcgtttga tttgggcatt acgcactttg atttagccaa caattacggg
    11521 ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgggagga ttttgccgct
    11581 tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc cggcccttac
    11641 ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg
    11701 gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa
    11761 gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc
    11821 tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt
    11881 ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc
    11941 ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag
    12001 ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa
    12061 gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg
    12121 cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc
    12181 tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa
    12241 ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag
    12301 attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc cgataaatga
    12361 cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag
    12421 tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga taatgacgta
    12481 ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat
    12541 ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt
    12601 ccccagccag ttcagcttct ctttgttctc ggagatatcc agctgtgaga cgaaattctc
    12661 ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga
    12721 cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat
    12781 aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac
    12841 gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc
    12901 ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc
    12961 aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt
    13021 tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg aagggagaaa
    13081 aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat atcccaaaca
    13141 gaaacaaccg acgccaggca tccaggcgaa gatgacaccg gtaccggatt gcggcgagaa
    13201 aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc
    13261 cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag
    13321 ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg
    13381 acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt
    13441 tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca
    13501 ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat
    13561 taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc
    13621 aagtatcatc accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta
    13681 tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga
    13741 gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc
    13801 cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga tgaagcgtgc
    13861 ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta
    13921 cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg
    13981 gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc
    14041 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    14101 tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    14161 gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt
    14221 aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    14281 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    14341 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    14401 aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    14461 tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    14521 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    14581 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    14641 gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg
    14701 caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta
    14761 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    14821 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa
    14881 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga
    14941 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    15001 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    15061 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg cgcttcagcc agcagttgct
    15121 gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg
    15181 cccaagtgac tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    15241 cgtgctgata cataccccag acggaaaggt ccgtctgcat caaattatta cccactgtgt
    15301 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaatgac gtcgaattga
    15361 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    15421 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    15481 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    15541 cccgagatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    15601 tacactctcg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt ctttgacagt
    15661 gtggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    15721 cgcggaacgt taacccattt ctccttacaa caagcgatgt gtgaactgga aggtggcgca
    15781 ggctgcgtgc tatttccctg cggggcggca gcggttgcta attccattct tgcttttgtc
    15841 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    15901 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    15961 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccaggctcc
    16021 atcaccatgg aagtccacga cgttccggcg attgttgccg ccgtacgcag tgtggtgccg
    16081 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    16141 tttggcatcg atgtttctat tcaagccgcc accaaatatc tggttgggca ttcagatgcg
    16201 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaacgcctat
    16261 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    16321 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggctga atggctggca
    16381 gaacatccgc aagtagcgcg agttaaccac cctgctctgc ctggcagtaa aggccacgaa
    16441 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    16501 ctcagtaatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    16561 tcgtggggcg ggtatgaatc gttgatcctg gcgaatcaac cagaacatat cgccgccatt
    16621 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    16681 gatgtcgacg atctgattgc cgatctggac gccggttttg cgcgaattgt ataacattgc
    16741 cacttttgga caattttgca gacatttaat tgtgaaaagt cttaaattgt tgcgtccggg
    16801 atcaaggcgt cccggacgaa tcaggagtac aataggcaga taaaggctta aacgctgttc
    16861 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    16921 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    16981 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact
    17041 ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac
    17101 cgccgcagca agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac
    17161 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    17221 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    17281 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt
    17341 caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag
    17401 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    17461 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtagtgatta aaaaaaaata
    17521 ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc
    17581 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    17641 actcacggct aaattgcaat gcgctttcat agtcgacgcg catcgctgct gcgctggcct
    17701 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    17761 gcaacggcga ggtgctggtg acagacttaa aattatgatg gaacgccgat acgctcatgt
    17821 tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaatccgtt
    17881 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    17941 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    18001 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    18061 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    18121 cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaatc
    18181 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    18241 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    18301 gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    18361 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    18421 tcagtttatt aactttatcc gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta
    18481 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    18541 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    18601 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    18661 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    18721 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggcggcg
    18781 gcagcgtgaa aaaaatcggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    18841 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    18901 aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg
    18961 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    19021 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    19081 tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg
    19141 acaagcaggc gttccattct gcccatgttc agccggtatt tgccgtgctc gatccggttt
    19201 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    19261 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    19321 gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    19381 atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg attggcgctg
    19441 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    19501 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgaga
    19561 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    19621 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    19681 tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    19741 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ttggatgtca
    19801 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    19861 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    19921 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    19981 ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    20041 agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga acgaagaagg
    20101 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    20161 gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    20221 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    20281 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    20341 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    20401 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcctggaa
    20461 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    20521 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaagccc cggcgcagat
    20581 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    20641 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcga
    20701 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    20761 acatgcaaat tctcccggtg acggtaatgt tccgctaccg gacttttcag aaatcattta
    20821 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    20881 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    20941 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    21001 ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg cagatttatc
    21061 agaactataa atcagatgcc agtacacctt atttcggtga aaccggagag cgggcgataa
    21121 cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata
    21181 ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag
    21241 aaagcgttgc gataacccaa actgacgccg atcatgccga tgccgttgtg cgctttcgta
    21301 tctttaaaga tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc
    21361 gttgggtcat tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg
    21421 agaatgaaaa aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg
    21481 ttgccgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg
    21541 actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc
    21601 ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg
    21661 aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg
    21721 cccgcattca tgttcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt
    21781 tacagcggcg cgaaggcaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc
    21841 tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata
    21901 acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg
    21961 agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca
    22021 actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc aggatataaa
    22081 agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct
    22141 tgatgctcag gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa
    22201 aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag
    22261 ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg
    22321 tagcaggcgt ctgacgctgg gtcgccatcg gcgtatgttt agtcaacgcc ggacgggtat
    22381 tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat
    22441 cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgccggg cggattaacg
    22501 gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc
    22561 ccttcggtac gaagacgtct tcgctcttat aaccaatctt cgccagcggt tttttcccgg
    22621 tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga
    22681 gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca tcacgcgtac
    22741 cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc tgtttcgagt
    22801 aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg
    22861 acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg acgtgatggg
    22921 tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg
    22981 cgatgaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg
    23041 gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac
    23101 aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac
    23161 cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat
    23221 taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac
    23281 agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt
    23341 aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa
    23401 aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc
    23461 ggtcaccgtg tttttgcatc aatgcgcgga cgcagcctgg gttggtttcg tggtgcagaa
    23521 tacgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca
    23581 acacgtaggt tttttcccac ggtttcgggc gcggtggctg cacggttacg gctttggctt
    23641 cctgcttttt cggtgccacc ggtttgttat ccgcgcacgg caaatcttca ccatacggat
    23701 gcgggattgg gtcgattttt ccaggggtat caagacgggt ggaatccacg ccgctccagc
    23761 caggcagcgc ctctttcacg ataatcgcgg tattacgcac atcgcggatt tcactaatcg
    23821 gctcgcccat cgccagacgg tgcgccacct ccaccagcgg acgctcaccg ttaccaaaca
    23881 tcagcatgtc ggctttcgaa tccaccagca cggaacggcg cacggtatcg gaccagtaat
    23941 cataatgtgc ggtacggcgc agactagcct caataccgcc gaggatcacc ggtacatctt
    24001 tccacgcctc tttacaacgc tgggtataaa ccagtgtggc gcgatccggg cgcttacccg
    24061 cgacgttatc cggcgtgtag gcatcgtcat gacgtaaacg gcgatcggcg gtataacggt
    24121 tgatcatcga atccatgttg ccagcagtaa caccgaaaaa cagattcggt ttacccagac
    24181 gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg cgaaagccct
    24241 gtgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt
    24301 cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag
    24361 acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca
    24421 ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa
    24481 aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaaa gtattccata
    24541 ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgactaaca gttgcccaat
    24601 cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca
    24661 ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt
    24721 ccagcctctg tcttccggga acggcatcgc accattcacg ttacggatct ggaacatcac
    24781 gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc
    24841 ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc
    24901 atcacccaga ctgatatcgc gactgcgaat cggcgaaccg gccatgattt cagttggcag
    24961 caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag
    25021 caccagggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat
    25081 gctcgccgct tcgccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat
    25141 ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa
    25201 tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg
    25261 acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta
    25321 cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc
    25381 gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc
    25441 atccacatca taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca
    25501 cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata
    25561 ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga
    25621 catcatccgt gccggaccgc ccatcagcgg gccaggtacc tgtagcccgg ccaccgtcat
    25681 tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac
    25741 gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg
    25801 cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc
    25861 aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac
    25921 agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat
    25981 atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa
    26041 tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc
    26101 tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct
    26161 ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc
    26221 agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg
    26281 cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg
    26341 ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg
    26401 acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg
    26461 gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc
    26521 accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta
    26581 gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc
    26641 aggccgaaca gcggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg
    26701 ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc
    26761 acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg
    26821 cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg
    26881 tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg
    26941 gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct tgcaagcggg
    27001 aggaaacagg ccctccccgg catattactc ttcgctatca ccgttgctgg cacggcgagg
    27061 agagtcgatc tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc
    27121 acgttcacca gtgactttct gtaactcttc cgggcgcagt ttaattttgc gtttcccaac
    27181 atgaatggtc agcgtgcttt gcggcggcag aacgtacaat tgcgccagac catcttctcc
    27241 acgcgcggct tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg
    27301 cggcagatcg cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat
    27361 atcggaagca tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat
    27421 caaagcctta cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta
    27481 acccgcatcg gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac
    27541 ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt tcgccctgac cacgcgccga
    27601 cggcagcgta atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccga
    27661 ttggttgctc ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc
    27721 cggcgcgtcg atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat
    27781 ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc
    27841 ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt
    27901 cagcaggtta ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt
    27961 ttccagctca ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag
    28021 tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc
    28081 cggtttcggt tcatcttcgt tacgaatgat ctcaatcact tcgtcgatgt tgagaaacgc
    28141 caccagcaaa ccttccagga tatgcaggcg cttgaggact ttctccagac gatagttcag
    28201 tcggcggcgc acggtatcgc gacggaacac cagccattcg gagaggattt ccagcaggtt
    28261 tttcaccgcc ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc
    28321 cagatcggtg gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg
    28381 cggcacaatc accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac
    28441 catcggcagc tttttgttgc gcatttgcgc agcaatttgc tccagtacac gcgcacctga
    28501 aacctgatgc ggtaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg
    28561 catacgcact gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat
    28621 aatttccgct tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt
    28681 ggttttcggc tggtcgatta atgcgattgc cgcctgagct acttcacgca ggttatgcgg
    28741 tggaatatcg gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg
    28801 cagacgagca ggcagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc
    28861 agccgtcccc tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc
    28921 ggtgtaacgc attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc
    28981 atcaaccagc ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca
    29041 ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt
    29101 tttaaatttg gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg
    29161 aacaggtttc agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga
    29221 gtagtttaag taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct
    29281 cattaattct gattcctcaa ctgattcgcc agccttcaaa ggggcaatat tgccggagat
    29341 actacattat ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg
    29401 ctccgacggc gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacttga
    29461 tcggcataga gggcagcaca aggttgcgcc catgccacac cactcggcat gggggtccgc
    29521 tccgggcgat tcgagaagtt gaggttatgc gagtccaggg aagcctaaag catcaaagta
    29581 gtcgactgta agtatatggt tcagcccagt ttagctgtgt ctccaccagt gccccgcgcc
    29641 tcctgccata agtgcggctt gctcatgggt acccccgaga cggcttacgg cgcaccggat
    29701 aataaatttc gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata
    29761 cgcctgtggc attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag
    29821 attccccatc acgctaacgg cacataaaca tccgtttcca gtcgctgctc cgtcacccct
    29881 tcggcaagat tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca
    29941 ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaacg
    30001 tggcgggcca cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta
    30061 tcgggaatcg gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg
    30121 gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc
    30181 gtctctttac gccacatgat aaatttcgcc acactgtagt tgagcaactc agggctacag
    30241 cggtgcggca acatcgccac agggattgat gggaaatcga taatgtttac atccagtgtc
    30301 aggtttgtca ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa
    30361 caattacgga tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt
    30421 atcgacttca ccctgaatct caaccgtatc tttcggcgtc accgtcacac cgttccagcg
    30481 tttgtggtcg atatcaacat tgatagtacc gctggcatct ttaaacacgt agagatcgtc
    30541 agagatgcgt tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt
    30601 tgcgctttct acagtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg
    30661 cgttgccgat gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cactgcacag
    30721 ggccattact gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg
    30781 tttccgagca tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta
    30841 atgctgtaca gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga
    30901 tgaaaaaatg cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac
    30961 tggccttagt aaaatgggtt ttagtgtcga ctggtttaca caaggtcgtc agggaaaaga
    31021 ggcgctttat agcgcacctt atgatgcggt gatcctggat ttaaccttac caggcatgga
    31081 tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag cgtgagccgg tactgatcct
    31141 gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg cgtctgggag ctgacgatta
    31201 tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg ctggaggctc tgatgcgccg
    31261 aaccaacggc caggccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa
    31321 acgtatcgcc acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct
    31381 ggaattactg atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact
    31441 gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg
    31501 acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga
    31561 gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat ctttttaatt
    31621 ctggcctcgg tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac
    31681 gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca agcggttaag tacgctcgat
    31741 ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt
    31801 catgttgatg acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt
    31861 aatgatggcg ataacggaga agatattccc tatagctatc aacgggaagg ttttgctgac
    31921 gggcaactgg tcggtgaaga cgatccttgg cgttttgtct ggatgacctc acctgatggc
    31981 aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg aagacatggc gctggcgatt
    32041 gttgccgggc aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg
    32101 gtactactgg gtcgtgaact cgcgccgctg aacaaactgg cgctggcact acgtatgcgt
    32161 gaccctgact cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcgaccactg
    32221 gttgagtcgc taaatcaact gttcgcccgc acacatgcga tgatggttcg agaacgacgc
    32281 tttacctccg atgcagctca cgaactacgt agcccgttaa cagcgctgaa agtgcaaacc
    32341 gaagttgcgc agctctctga cgatgatccg caggcgcgga aaaaagcact gctccaatta
    32401 cattccggga tcgatcgcgc tactcgtctg gttgatcaac tgctcacgct atcgcggctg
    32461 gactcactgg ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctgcaatcg
    32521 tcggtgatgg atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc
    32581 aatgcccaca gcatcaaacg cactggacaa ccgctattgc taagtttgtt ggtgcgaaac
    32641 cttctggata acgccgtgcg ctacagtcca cagggcagcg tggtggacgt cacgctgaat
    32701 gccgataatt tcatcgtgag ggataacggc cccggtgtga caccagaggc actggcgcga
    32761 attggcgaac gcttctatcg cccccccgga caaaccgcta ccggcagtgg acttgggcta
    32821 tcgattgtcc agcgaatcgc caaattgcat ggcatgaatg ttgaatttgg gaatgcggaa
    32881 caaggtggat ttgaggcgaa ggtgagctgg taagggtaag acttttgcta aattcgcaaa
    32941 agactttgca cattttgcta attttaccgt accgctctgt gacgtactat agtcgacaaa
    33001 cgtctcacct tgaggttaaa aaatgagcaa caccctgatt atcaacggcg cgaaaaaatt
    33061 cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg
    33121 cgacctcggg catgatgttc gcatcgttcg cgccgacagc gactacgatg tcaaagcgga
    33181 agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct ggtggatggg
    33241 cgcgccgtgg acagtgaaaa aatacattga tgatgtattc accgaaggtc acgggacgct
    33301 gtatgccagc gatggccgta cccgcaaaga tccgtcaaaa aaatacggtt ccggcggcct
    33361 ggtacagggc aaaaaatata tgctttctct gacctggaac gcaccaatgg aagccttcac
    33421 cgaaaaagat caattcttcc acggcgttgg cgtcgacggt gtgtatctgc cgttccataa
    33481 agcaaaccaa ttccttggca tggaaccgct gccgacattt atcgctaatg acgtgataaa
    33541 aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg
    33601 ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg cagaaatccg
    33661 tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta aaatcgttcc
    33721 aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg
    33781 cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat
    33841 cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagcg aagccgtaaa
    33901 aggcgacgtg ctggagatga atattcgtat tctgcagcca gggatttaat cctgccttgt
    33961 ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa acctcaatct ccgccatgtc
    34021 gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca gcagcatatc
    34081 catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca ccagacggcg
    34141 agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc ccagaccttt
    34201 aaaacgctgg acgttcggct tgcctttctt gcgttttaat tgctcaagta cgccctcttt
    34261 ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga tcaatacggt agagcggtgg
    34321 cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta cgaacaaagc
    34381 gcagagcagc gtggcaatgt gcagaccatc agagtccgca tccgcgagga tacagatttt
    34441 gccataacga agctggctca gatcgtcgct gtcaggatcg ataccgatcg ctaccgaaat
    34501 atcatgcact tcctgcgagg ccagcacttc gtcggaagaa acttcccagg tgttaaggat
    34561 cttacctttc agcggcatga tcgcctgata ttcacgatcg cgcgcctgct tggcagatcc
    34621 gcctgcggag tcaccttcca cgaggaacag ttcggtacgg ttaaggtcct gcgcggtaca
    34681 atcagccagt ttgccaggca gcgccgggcc gctggtcagc tttttacgca ccactttttt
    34741 agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccagca actccgccgc
    34801 ctgaacgttc tggttcagcc acaggataaa ggcatctttc accacgccag aaacgaatgc
    34861 cgcgcattga cgcgaagaga gacgctcttt cgtctgcccg gcaaactgcg gatcctgcat
    34921 tttcaccgac agaacatagg cgcagcgatc ccagatatct tccgccgaca gcttcacgcc
    34981 gcgcggcaga atgttgcgat attcacagaa ctcacgcatc gcgtccaaca ggccctgacg
    35041 caggccatta acatgggtac cgccctgcat cgttgggatc aggttgacgt agctttcggt
    35101 cagcagttca ccgccttccg gcagccacag tagcgcccag tccacagctt cagtatcgcc
    35161 agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta ccgcttccgc
    35221 caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt tgatctcatc
    35281 tttaaaggta atctcaacgc cggggcacag taccgctttg gctttcagca catgcgtcag
    35341 gcgtgaaaca gaaaatcgcg ggctgtcaaa gaaggtttca tccggccaga agtgcacgct
    35401 ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca ccttttcgcc
    35461 attttcaaag gcgatgttat aaacctgacc atcgcggcgc acgttaactt caacgcgctt
    35521 cgacagggcg ttaaccaccg aaatccccac gccatgcagg ccgccagaga actggtaatt
    35581 tttgttagag aatttaccgc ctgcatgcag acggcaaaga atcaattcaa ccgccgggac
    35641 accctcttcc gggtgaatat ccaccggcat cccgcgccca tcgtcaataa cttctaacga
    35701 ctggtcagca tgtaagatta catccacgcg ttttgcgtga cccgccagtg cttcatcgac
    35761 actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg tatacatccc
    35821 cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag cgttataagt
    35881 ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc agaccaagaa
    35941 aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg ccgccttcta
    36001 tgacagtctg gcggcaggaa gcgtagtacg ccaccgcctg gcggtaatcc agcacttcat
    36061 ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca atctgcatga
    36121 ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcccggtg tatgggttct
    36181 cgttctgacc gagatagtcc gtcagcagtt caaacgggcg caccgccggg tttaccacca
    36241 ctgcgggcag cataaaacat tgtgacaacc aggtggcgta atatcccccc agtgacgaac
    36301 cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc agttctgccg
    36361 cgtcggaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg tgatgttccg
    36421 ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg aaaccgtgta
    36481 aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga aacgtgtgtc
    36541 cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga gagtacgcca
    36601 gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact gcacacaggt
    36661 cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct gatgaatatg
    36721 accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca gttcgcccgc
    36781 gttacgcaga ctgtgttgat cgagccaact acaacccgca ggtagcggat gatgatgcag
    36841 cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc actcaagctg
    36901 aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca acaggatttg
    36961 ccattgctca ccaataaaca cccgcttcgc cggggagata cccgcatcct gtaacgcgct
    37021 gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg cacgaaaact
    37081 tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg ctaaatcacc
    37141 tgtcgcgaca atcaggtcga attcgtgctg atgtggacga atcgcctcca gcaccgcctg
    37201 gtagctttcc caggtgttta cccctaacag agcttcgtgc ttttgtgcaa acaggtgagt
    37261 gtcggtaatt tgtaaaatcc tgactctggc ctcaccagcc agaggaaggg ttaacaggct
    37321 ttccaaatgg tgtccttagg tttcacgatg ctagtaaacc ggaatcgcca tcgctccatg
    37381 tgctaaacag tatcgcaacc agtccgctaa aaactgatta atttgatgct tttcgtcgcg
    37441 ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa tctgctggct
    37501 tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg acggaaggct
    37561 ccagtaactg atcgcgggcg cagtctgttc tattgtcacc agggtagtgt atcgggtcga
    37621 ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag tttcgccggg
    37681 tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc acaggcgcat
    37741 catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt ttaacgcttg
    37801 atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga ttttcccctc
    37861 ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat cttcgttttc
    37921 atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca ccataattga
    37981 cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt tggtccgttt
    38041 gactatcagt cccgcctctt caatcgcttc gcgacgggca acatcttcca cactttcacc
    38101 ctcttctatc atcccggcaa ccatctccag tagccagggg gtttcactgg tgtcgtatgc
    38161 ggcaatccga atctgctcaa tcagcacaac ttcatcgcgc actgggtcaa agggtagcaa
    38221 gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca tttgcccgtt
    38281 gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat aaagtgtttc
    38341 tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt ctggcttaag
    38401 cattgttaat gtcctggcac taatagtgaa ttaaatgtga atttcagcga cgtttgactg
    38461 ccgtttgagc agtcatgtgt taaattgagg cacattaacg ccctatggca cgtaacgcca
    38521 accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt gatcgcgcta
    38581 aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct tatcggcctg
    38641 agcctttctg ggttcagttc gttgagccag gccgagaacc taatgcaagt ttatcagcaa
    38701 gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc tgcctttgaa
    38761 aaaattaatg aagcgcgcag tccattactg ccacagctag gtttaggtgc agattacacc
    38821 tatagcaacg gctaccgcga cgcgaacggc atcaactcta acgcgaccag tgcgtccctg
    38881 cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct gcaggaaaaa
    38941 gcagcaggga ttcaggacgt cacatatcag accgatcagc aaaccttgat cctcaacacc
    39001 gcgaccgctt atttcaacgt gttgaatgct attgacgttc tttcctatac acaggcacaa
    39061 aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt gggcctggta
    39121 gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc gaacgaagtg
    39181 accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac cggtaactac
    39241 tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc acagccggtt
    39301 aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc acgcttgagc
    39361 caggacctgg cgcgcgagca aattcgccag gcgcaggatg gtcacttacc gacgctggat
    39421 ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac ccgtggtgcc
    39481 gctggtaccc agtatgacga cagcaatatg ggccagaaca aagtgggcct gagcttctcg
    39541 ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggccca gtacaacttt
    39601 gttggtgcca gcgagcaact ggaaagcgcg catcgtagcg tcgtgcaaac cgtacgttcc
    39661 tccttcaaca acattaatgc atctatcagt agcattaacg cctacaaaca agccgtagtt
    39721 tccgctcaaa gctcattaga cgcgatggaa gcgggctact cggtcggtac gcgtaccatt
    39781 gttgatgtgt tggatgcaac caccacgctg tacaacgcta agcaagagct ggcaaatgcg
    39841 cgttataact acctgattaa ccagttgaat attaagtcag ctctgggtac gttgaacgag
    39901 caggatctgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa tccggaaaac
    39961 gttgcaccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc tgatagcccg
    40021 gcaccagtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca taaccctttc
    40081 cgtaactgac gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa cgtaaagata
    40141 cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc atcctctatt
    40201 ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa atccatacgc
    40261 cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc tctcgcggtt
    40321 gccactgttt ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc tctctatcaa
    40381 aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac caccgcgtac
    40441 aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg tgaagactgt
    40501 gttgctgaat ttggtgaagg tcagtgccag caggcaccag cccaggctgg catggcacca
    40561 gaaaaccagg cgcaggccca gcaatccagc gggagtttct ggatgccgct gatggccggt
    40621 tacatgatgg ggcgtctgat gggcggcggc gcgggatttg cacagcagcc gctgttctcc
    40681 tcgaaaaacc cagccagccc ggcttacggt aaatataccg acgcgacggg taaaaactat
    40741 ggcgcagccc agccaggccg caccatgacc gtaccgaaga cagccatggc accaaaaccg
    40801 gcgaccacta ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa acaaagcact
    40861 atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata ccgatggaaa
    40921 gagtcagtat taccgagcgc ccggactggc gcgaaaaagc ccacgaatac ggtttcaatt
    40981 ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag ttgaccctcg
    41041 cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc ctgaaagtgg
    41101 tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcattcca aaacgcacct
    41161 ggagttttgt gcgtcagtca tggctgactc accagccatc gctttattcg cgtcttgatc
    41221 tggcatggga tggcactggc gaacctaagc tgctggaaaa taacgccgat acaccaacgt
    41281 cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt aacgccggta
    41341 acttgccgga gggcagcgac cagtttaaca gtctgcaaga aaagctaatc gatcgctttg
    41401 tcgagttgcg tgaacagtat ggcttccagt tgctgcatct cacctgctgt cgcgacacgg
    41461 tggaagatcg cggaaccatt cagtatttgc aggactgcgc gacggaagct gaaatcgcta
    41521 ccgagttcct ctacattgac gatatcgggt taggtgaaaa aggtcagttc acggatttac
    41581 aggatcaggt aatttccaac ctgttcaagc tgtatccatg ggaatttatg ttgcgtgaga
    41641 tgttctccac caagctggag gatgcaggcg tacgctggct ggaaccggcg tggaagagca
    41701 ttatctccaa caaagcgctt ctaccgctac tgtgggagat gttcccgaat cacccgaacc
    41761 tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat gtggttaaac
    41821 cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa accattgaag
    41881 cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac ccgttaccga
    41941 aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa cctgccggaa
    42001 ttggcattcg tgaagaccgt gcgttgatca cccaggatat gtctcggttt tatccacata
    42061 tttttattga gtaatcaatg atgccggatg gcattcgcca tccggtaatt gttaacctat
    42121 ctgcaccgac agcatactca ggctgcccat ttctatacca tcgaccggaa tggtaattgg
    42181 ctcctgccca tcccacgcac ctaagacata caacaacggc agatagtgct ccggcgttgg
    42241 gttcgataac gcgccacctt catggtcaag gtaattcacc agaggatgct gctctaccgc
    42301 cccttgccac gtcagattcg ctttcacata ctcattaaac gacatcgccc aaggatatgg
    42361 tgaactatca ccgtgccact tcactgtacg caggttatgc accacgttgc cactggcgac
    42421 caacattatt ccttcatctc gcagcgctgc cagtttgcgc cccatttcga aatgccaggc
    42481 ggcaggtttg ctactgtcga tactcaactg caccatcggg atatcggcgt caggatacat
    42541 cttaatcagc acgccccagg aaccgtggtc aaagccccag gcttctttat ccagcgtcac
    42601 cgggaccgac gctaacagct caaccagatg ctgtgccagc acaggcgaac ccggagcagg
    42661 ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcgt
    42721 ctccatcgcg gtcactcctg ttccacgggt aaaccagtga gccgaaacca ccacaatcgc
    42781 ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg cgggtataca aattatcttc
    42841 cagcacgttc atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat
    42901 gatgatatcc ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga
    42961 gtaagaaccc ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt
    43021 gaagatttat aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc
    43081 agccacgttt attggcgcgt ttctcggcgt tctcgggcaa aaaccctcga accgcttact
    43141 ggcgttttcg ctaggatttg cggcggggat catgttgctc atctcattaa tggaaatgct
    43201 tcctgccgca ctagcagctg aaggaatgtc gcctgtgttg ggttatggaa tgtttatctt
    43261 cggtctgctt ggctattttg gcctggaccg catgttgcca catgcgcatc cgcaggattt
    43321 aatgcaaaaa tcggtgcagc cgttgccaaa atcgatcaag cgcacagcca ttctgctcac
    43381 tctcggcatc agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag
    43441 caacctggag ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata tccctgaggg
    43501 tctggcagtg gcaggcccgg tttatgcggc aacggggtct aaacgcaccg cgattctgtg
    43561 ggcggggatt tctggactag cagaaattct tggtggtgtg ctggcgtggt taatcctcgg
    43621 tagcatgatt tccccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt
    43681 ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc
    43741 cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac
    43801 ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag
    43861 cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt
    43921 tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca
    43981 aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa
    44041 acatcctttt acccttaata tttaacaata ctaaacattt agcgcataaa tttcacatat
    44101 cctttttcgg atatattcat atggtcgcat aataacggaa caattatatg attttgatca
    44161 taatgaaaaa gaatgcctga tattaatgaa attttctcat agaaagagaa gcggaattaa
    44221 catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg tttcttgatt
    44281 aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct gacggtatgc
    44341 caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact caatgcactc
    44401 tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac cagccggttt
    44461 aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac gcgtcagtac
    44521 accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat cagaaggttt
    44581 tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag aaacaccggt
    44641 agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt
    44701 attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga tgcacaggca
    44761 aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca tggtttctgc
    44821 cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca ttacaccgcg
    44881 tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac caaaagagga
    44941 aagtagcgtc tgattcatgg taaaaaaacc tcactaaaat tatggttacc agaatcaggg
    45001 cagtcttagg agtggcggca tatagccaaa ataacgtgag cgggtccatg cccgacagaa
    45061 tcgttactct ctcccatccg gactctaacc gtcggccccg gaattacacc ggatctgctg
    45121 tcctttgagt tcgcacccaa agcgctcgcg ggctttcaac tgagttgatt taccgccggt
    45181 ggggaatttc gccccgccct gagaataagc ggattcacta taacgctaat gattagcggc
    45241 agcaacgcat agcttcacat aattctggtt tatgacttac ccttatcgca ctacaatggt
    45301 actcaacacc tatcagtaca gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat
    45361 cgctcgccag gttcacgaat caatgcctaa aggaatcagg gagttcgggg aagatgtgga
    45421 gaaaaaaatc cgccaaaccc tacaagctca gctgacgcgc ctcgaccttg taagccgtga
    45481 agagttcgac gtccaaacgc aagtcctgtt acggactcgt gagaaactgg cgcggcttga
    45541 acaaagaatg agcgaactgg aaaatcgcag tactgagatt aaaaagcagc cagatccaga
    45601 gactctcccc ccaacgctgt aaacatttgt ctttaatgca ctgtaaatta tgaattaatc
    45661 ttctaattta cagtgctttc tattacctta tcgcttaatt ccattca
//
LOCUS       DAZOYS010000011        44695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOYS010000011 DAZOYS010000000
VERSION     DAZOYS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.011"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUN9_000513"
     CDS             complement(<1..716)
                     /locus_tag="ACHUN9_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0694024.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUN9_000514"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUN9_000514"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0694025.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUN9_000515"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUN9_000515"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0694026.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUN9_000516"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUN9_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0694027.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUN9_000517"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUN9_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0694028.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUN9_000518"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUN9_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0694029.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHUN9_000519"
     CDS             complement(6604..7446)
                     /locus_tag="ACHUN9_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0694030.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHUN9_000520"
     CDS             complement(7433..8314)
                     /locus_tag="ACHUN9_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0694031.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHUN9_000521"
     CDS             complement(8335..9645)
                     /locus_tag="ACHUN9_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0694032.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHUN9_000522"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHUN9_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUN9_000523"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUN9_000523"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0694033.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUN9_000524"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUN9_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0694034.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUN9_000525"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUN9_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0694035.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUN9_000526"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUN9_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0694036.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUN9_000527"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUN9_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0694037.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUN9_000528"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUN9_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0694038.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUN9_000529"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUN9_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUN9_000530"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUN9_000530"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0694039.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUN9_000531"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUN9_000531"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR0694040.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUN9_000532"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUN9_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0694041.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUN9_000533"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUN9_000533"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0694042.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUN9_000534"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUN9_000534"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0694043.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHUN9_000535"
     CDS             21883..22047
                     /locus_tag="ACHUN9_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694044.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUN9_000536"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUN9_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0694045.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHUN9_000537"
     CDS             23556..23801
                     /locus_tag="ACHUN9_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0694046.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUN9_000538"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUN9_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0694047.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUN9_000539"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUN9_000539"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0694048.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUN9_000540"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUN9_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0694049.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUN9_000541"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUN9_000541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0694050.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUN9_000542"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUN9_000542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0694051.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHUN9_000543"
     CDS             29464..29817
                     /locus_tag="ACHUN9_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0694052.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUN9_000544"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUN9_000544"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0694053.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHUN9_000545"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHUN9_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUN9_000546"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUN9_000546"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0694054.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHUN9_000547"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHUN9_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUN9_000548"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUN9_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUN9_000549"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUN9_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0694055.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUN9_000550"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUN9_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0694056.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHUN9_000551"
     CDS             36881..37630
                     /locus_tag="ACHUN9_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0694057.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHUN9_000552"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHUN9_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0694058.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHUN9_000553"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHUN9_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0694059.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHUN9_000554"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHUN9_000554"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0694060.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHUN9_000555"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHUN9_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0694061.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHUN9_000556"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHUN9_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0694062.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHUN9_000557"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHUN9_000557"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0694063.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHUN9_000558"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHUN9_000558"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0694064.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..>44695)
                     /gene="acnA"
                     /locus_tag="ACHUN9_000559"
     CDS             complement(42720..>44695)
                     /gene="acnA"
                     /locus_tag="ACHUN9_000559"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0694065.1"
                     /translation="AAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKH
                     GVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQ
                     VELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFA
                     ASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAK
                     KAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGP
                     LPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINL
                     ASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVT
                     RSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIK
                     PDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHL
                     PDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFER
                     IHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQ
                     EVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10537 a        11833 c        11286 g        11039 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggctt
//
LOCUS       DAZOYS010000012        44195 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOYS010000012 DAZOYS010000000
VERSION     DAZOYS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.012"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHUN9_000560"
     CDS             complement(<1..560)
                     /locus_tag="ACHUN9_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0694066.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(763..999)
                     /gene="feoC"
                     /locus_tag="ACHUN9_000561"
     CDS             complement(763..999)
                     /gene="feoC"
                     /locus_tag="ACHUN9_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0694067.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(999..3320)
                     /gene="feoB"
                     /locus_tag="ACHUN9_000562"
     CDS             complement(999..3320)
                     /gene="feoB"
                     /locus_tag="ACHUN9_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0694068.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="ACHUN9_000563"
     CDS             complement(3337..3564)
                     /gene="feoA"
                     /locus_tag="ACHUN9_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0694069.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4002..6323)
                     /locus_tag="ACHUN9_000564"
     CDS             complement(4002..6323)
                     /locus_tag="ACHUN9_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0694070.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6420..6896)
                     /gene="greB"
                     /locus_tag="ACHUN9_000565"
     CDS             complement(6420..6896)
                     /gene="greB"
                     /locus_tag="ACHUN9_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0694071.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7124..7843
                     /gene="ompR"
                     /locus_tag="ACHUN9_000566"
     CDS             7124..7843
                     /gene="ompR"
                     /locus_tag="ACHUN9_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0694072.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7840..9192
                     /gene="envZ"
                     /locus_tag="ACHUN9_000567"
     CDS             7840..9192
                     /gene="envZ"
                     /locus_tag="ACHUN9_000567"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0694073.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="ACHUN9_000568"
     CDS             complement(9268..10890)
                     /gene="pckA"
                     /locus_tag="ACHUN9_000568"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0694074.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11269..12969
                     /locus_tag="ACHUN9_000569"
                     /pseudo
     CDS             11269..12969
                     /locus_tag="ACHUN9_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="ACHUN9_000570"
     CDS             complement(13104..13982)
                     /gene="hslO"
                     /locus_tag="ACHUN9_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0694075.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="ACHUN9_000571"
     CDS             complement(14007..14408)
                     /gene="hslR"
                     /locus_tag="ACHUN9_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0694076.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="ACHUN9_000572"
     CDS             complement(14419..15087)
                     /gene="yrfG"
                     /locus_tag="ACHUN9_000572"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0694077.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="ACHUN9_000573"
     CDS             complement(15152..17287)
                     /gene="igaA"
                     /locus_tag="ACHUN9_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0694078.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17607..18167
                     /gene="nudE"
                     /locus_tag="ACHUN9_000574"
     CDS             17607..18167
                     /gene="nudE"
                     /locus_tag="ACHUN9_000574"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0694079.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="ACHUN9_000575"
     CDS             complement(18332..20884)
                     /gene="mrcA"
                     /locus_tag="ACHUN9_000575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0694080.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21004..21783
                     /gene="hofM"
                     /locus_tag="ACHUN9_000576"
     CDS             21004..21783
                     /gene="hofM"
                     /locus_tag="ACHUN9_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0694081.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21783..22322
                     /gene="hofN"
                     /locus_tag="ACHUN9_000577"
     CDS             21783..22322
                     /gene="hofN"
                     /locus_tag="ACHUN9_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR0694082.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22306..22746
                     /gene="hofO"
                     /locus_tag="ACHUN9_000578"
     CDS             22306..22746
                     /gene="hofO"
                     /locus_tag="ACHUN9_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0694083.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22736..23140
                     /gene="hofP"
                     /locus_tag="ACHUN9_000579"
     CDS             22736..23140
                     /gene="hofP"
                     /locus_tag="ACHUN9_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0694084.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23052..24290
                     /gene="hofQ"
                     /locus_tag="ACHUN9_000580"
     CDS             23052..24290
                     /gene="hofQ"
                     /locus_tag="ACHUN9_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0694085.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24691..25212
                     /gene="aroK"
                     /locus_tag="ACHUN9_000581"
     CDS             24691..25212
                     /gene="aroK"
                     /locus_tag="ACHUN9_000581"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0694086.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25269..26357
                     /gene="aroB"
                     /locus_tag="ACHUN9_000582"
     CDS             25269..26357
                     /gene="aroB"
                     /locus_tag="ACHUN9_000582"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0694087.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26449..27735
                     /gene="damX"
                     /locus_tag="ACHUN9_000583"
     CDS             26449..27735
                     /gene="damX"
                     /locus_tag="ACHUN9_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0694088.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27842..28678
                     /gene="dam"
                     /locus_tag="ACHUN9_000584"
     CDS             27842..28678
                     /gene="dam"
                     /locus_tag="ACHUN9_000584"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0694089.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28696..29373
                     /gene="rpe"
                     /locus_tag="ACHUN9_000585"
     CDS             28696..29373
                     /gene="rpe"
                     /locus_tag="ACHUN9_000585"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0694090.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29366..30124
                     /gene="gph"
                     /locus_tag="ACHUN9_000586"
     CDS             29366..30124
                     /gene="gph"
                     /locus_tag="ACHUN9_000586"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0694091.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30117..31121
                     /gene="trpS"
                     /locus_tag="ACHUN9_000587"
     CDS             30117..31121
                     /gene="trpS"
                     /locus_tag="ACHUN9_000587"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0694092.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31281..32186
                     /gene="yhfZ"
                     /locus_tag="ACHUN9_000588"
     CDS             31281..32186
                     /gene="yhfZ"
                     /locus_tag="ACHUN9_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0694093.1"
                     /translation="MRRTFIKKEGVVIMTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32203..32565
                     /locus_tag="ACHUN9_000589"
     CDS             32203..32565
                     /locus_tag="ACHUN9_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0694094.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32649..33812
                     /locus_tag="ACHUN9_000590"
                     /pseudo
     CDS             32649..33812
                     /locus_tag="ACHUN9_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33812..35038
                     /locus_tag="ACHUN9_000591"
                     /pseudo
     CDS             33812..35038
                     /locus_tag="ACHUN9_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35035..35913
                     /locus_tag="ACHUN9_000592"
     CDS             35035..35913
                     /locus_tag="ACHUN9_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0694095.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35924..36277
                     /locus_tag="ACHUN9_000593"
     CDS             35924..36277
                     /locus_tag="ACHUN9_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0694096.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36289..37593
                     /locus_tag="ACHUN9_000594"
     CDS             36289..37593
                     /locus_tag="ACHUN9_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0694097.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37605..38690
                     /locus_tag="ACHUN9_000595"
     CDS             37605..38690
                     /locus_tag="ACHUN9_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0694098.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38842..39573)
                     /gene="frlR"
                     /locus_tag="ACHUN9_000596"
                     /gene_synonym="yhfR"
     CDS             complement(38842..39573)
                     /gene="frlR"
                     /locus_tag="ACHUN9_000596"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0694099.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="ACHUN9_000597"
     CDS             complement(39673..40458)
                     /gene="frlD"
                     /locus_tag="ACHUN9_000597"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0694100.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="ACHUN9_000598"
                     /pseudo
     CDS             complement(40455..41286)
                     /gene="frlC"
                     /locus_tag="ACHUN9_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="ACHUN9_000599"
     CDS             complement(41336..42358)
                     /gene="frlB"
                     /locus_tag="ACHUN9_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0694101.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42379..43716)
                     /gene="frlA"
                     /locus_tag="ACHUN9_000600"
     CDS             complement(42379..43716)
                     /gene="frlA"
                     /locus_tag="ACHUN9_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0694102.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44011..44178)
                     /locus_tag="ACHUN9_000601"
     CDS             complement(44011..44178)
                     /locus_tag="ACHUN9_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0694103.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        10309 a        11210 c        12099 g        10577 t
ORIGIN      
        1 tcgcgttgac gaatatgttt ttgaattaac tccagcagcg ccatacgtcg gtgctgcatg
       61 atttcatcat caggcatgac ggtgacatca atcagcggaa atgcggaggc ataaagctgc
      121 cttgccagtt tgggatcggc gaaacaatcc agccaacaca gcgaataggg ataggggctt
      181 tcaataccgt ggtaaaacaa caatagcacc accatcggca acgttttgta tccagcatca
      241 aggtgatttt gcattgcggc aatagcgtaa cgcatcatgc gaaatgcgat cagtttgttt
      301 gaggtgcttt gatgttcaat cagacaatag atgtatcctg gtccttgttc cgttttcacc
      361 gaccacagca catcggaata gctttcacgc agatcgtcat caataaagct gctcgactcc
      421 agcttgaggg ttttcatatc acaaagcgcg tgaatgggtg ccggtaaatg aaacgcaaga
      481 aaatcacgag ccgtgtccgg ttggcgtaaa aagagtttga acagcgcatc gtgtggggtg
      541 gaactctgct ttttgctcat ggcgttcctt cacctgttgt ttgaatgccg cgactctacg
      601 cgccactcca cacctgtgca ttagcgagtt ttcatccctg cgagatgctt tcaaaggaac
      661 ttacgccgtg ctgcaagagt tgcattttta tggaaagccg cacgcaaaaa agcccggatg
      721 cagacgcata ccgggctttc caaaacaggc gatggagtaa ggttaacgca gcgcccacca
      781 ctcgcgcaga caggcttttc cttccgggca gcttttacaa ctgccagaga ggcagccgtc
      841 aggttcttcc tgaatccgca cggctttgcc catactttcc agttgttgca gcatggcgtt
      901 aatcattggc tgcggagtgt tcaatgtctg gcttatctgg gtcgcttcca tacggccccg
      961 taacgccagc aaatcacgta cctgaataag tgaagccatt aatggcaatc accggtggtg
     1021 ctggctgcgc agcaactgct taccgacttg cgggttgcca gcagttcgat atccacccgg
     1081 ctacgcgcgc ggcgcagcag accgataact acgatgttaa acaggattac cgccagaatg
     1141 cacaccaggc tgtaagttgg gtgctgactg tagctggcga cctgatagaa caatgttgcc
     1201 agtgagtaag cgatattcag cccccacagg atggagaagc ccatccagcc acggcttgat
     1261 tcacgggcga tggctcccat caccgagata catggtacat acagcaggac gaaaatcagg
     1321 tagctgtatg cggcagctgc gctaccgaat ttctgatcca tcacgcccat cgccccggta
     1381 cccatttcac cgtcgccttt gctagcttca atggggttca tcagtacgct aaggctgaag
     1441 gtgtctttca ggctctgcca ggtttcatct accgcactga acagctcttc accgaggttg
     1501 aattccgccg gattgaactc ttcgtcctga atattttctg cggtgtagag ggtgttgagc
     1561 gtacccacca ccacttcttt tgccattgcg cccgtaaaca ggccaaccgt tgcctgccag
     1621 ttatcttcat gtacgccaat cggcttgaag acaggggtta tcacccggct gacggacgcc
     1681 agcgccgagt cgttgatgtt atcgacgatt ttcccgctca acgagaagct gttgaaagcg
     1741 ctcaggaaaa tgctgacgat gataatcact ttaccagcac gcagaacgaa gcccttcaga
     1801 cgctgccagg tctggataat caggctttta acgtgtggta catggtagac cggcagctcc
     1861 atgacaaacg gcgtcgcttc accgcgcatg atggtgtact tgagcatcag gccagtcagc
     1921 accgccatca cgataccgag catatacagc gagaaaaccg ccagggcacc gttctgcccg
     1981 aagaaggcag ccgcgaatac tgcgaagata gccagacgcg cgccgcagga cataaacggt
     2041 gccatcatga tggtcatcag acgttcacgc ggtgcatcaa gcgtacgtgc gcccattacc
     2101 gacggtacgt tacaaccgaa accgacgatc agcggcacaa aggatttccc cggcaagccc
     2161 agcgcctgca tcaggcggtc catcacaaac gctgcacgcg ccatatagcc ggagtcctca
     2221 aggaaggaga ggaacaggta catcatgccg atctgcggca ccagtggcag cacggtgtta
     2281 ataccgccac ccaggccctg ggcgaggaag atagtcagcc agtccgggaa gtggagcgtg
     2341 tagccaatcc attgaatacc atgcacaaat agcgccacgg agccgacgtc aaacagcggc
     2401 tgtaacgccc cgccgatgtt gatagccagc aggaacatca ggtacatcac aaagaggaaa
     2461 atcggcagac cgaggaaacg gttgagcacg attttatcta ccgcagtggt gaaacggctg
     2521 ggttctgccg tcagggtgtt gcttaccaca tcacagatgg cagctatgca ctggtaacgc
     2581 gcatcggcaa tgtgcagcgc cggatcatcc atttcattac gcagacgggc aagggcggca
     2641 tccagatgct gcgacgcttc accggcgtag gcgcggctgt agatatcgcc ttccagcatt
     2701 tgcaggccca gccagcgacg ttgtttcagc gggatgtcgg aaggcatcac ttttgccagt
     2761 gaatctgctt cgttgagtag cggctgtgcg taatgcacca gttccacatt ctcgttagct
     2821 ttatagcgat caatcgccag cttgagcgct tcaataccgc gaccacgggt tgaaaccagc
     2881 gggatcactg ggcagccaag acgcgccgac agagcatcaa tttcaatacg aatattttgc
     2941 ttctcggcaa tgtcgagcat gttcagtgcc acgatgcagg gaatgccgag ttccagcagt
     3001 tgtagcgtca ggtacaggtt acgctcaagg ttagacgcat ccaccacgtt aatcagcagg
     3061 tcggcgtcgc cactcaaaat gtagtgacag gcgatttgct catcgagcga ggtctgcgat
     3121 gagatggtgg tcagagaata ggtgccgggc aggtccacca gcgtgacctg atgatcggtg
     3181 gtggagaatt gcccttcttt acgttcgacg gtaacgccag cccagttacc tacacgctga
     3241 cgtgcgctag tgagctggtt aaataaggtt gtcttgccag aatttggatt accaattaag
     3301 ccaatggtta attttttcat tgttgttatc accgtattaa caggaaaccg cttccacttc
     3361 taataaggcc agatcttttt tgcgtaatac caggctcaca cgacgggttt cgatatgaat
     3421 ggggtcgccg agtggagcga cgcgcaccac attaaaagag gagccaggta acatgccaag
     3481 agaaagcagt ttttggcgat atgccgggct gatttcacgg gaaaagccag tgattttcca
     3541 cgcagtatct ggagtgtatt gcataggtgc ctacttgttt ctcattaact ggataactac
     3601 ctcatggggc gtttgacagc gacatgagac cagatgattt gccaacaaag gagagattaa
     3661 ataatcaaca acacaatgat aatgagaatg gtttctatca gcaatactta aaatgtgtgc
     3721 gaatgttggt ttgaataata atctgactca atgttgatgt ggctcaaggt tatatcgcag
     3781 cgaaaatata attcgaatag tgaattaccc ggactggcta atgtttaatt aaggtttcag
     3841 ttgttaatga agtgataaat attgccggaa tatattatga gtttggttgt ggaataaaaa
     3901 ggccggagat attctccggc acttttatgt caggcaaggc gttcgttgcc agatgcggcg
     3961 tgaacgcctt atcgcgcagg tttaggggcc agtccgggcg tttaacgttt tttgcccatt
     4021 gccgccgcca gcgcatccat catcgcgcta ttaccggcag gctgcgcttc acgaccgcgt
     4081 ggtttggctg ccgggcggtt gttttgcggg cgttcattac cgccgccgcg acgggcgttg
     4141 gtttcgccag gctgctcgtc cagacgcatg gtcagggcga tacgtttacg ctgaagatcc
     4201 acttccagca ctttcacctt cacaatgtcg cccgctttca ccacggtatg cggatcttcc
     4261 acaaacttgt tcgacaatga agagatgtga accaggccgt cctgatgcac gccaatatcg
     4321 acaaacgcgc caaagttggt gacgttggtg acagcgcctt cgaggatcat acccggttgc
     4381 aggtcgttca ttgtctcgac gccatcggca aactgagcgg ttttaaattc cggacgcgga
     4441 tcgcgacccg gtttttccag ctctttgatg atgtcagtta ctgtcggcac accgaatttt
     4501 tcatcagtaa agtcagacgc tttcaggtta cgcagttcgc tgctgttacc catcagatct
     4561 ttcagtgcct gctgtgttgc tgccagaatg cgttccacca ccggataggc ttccgggtga
     4621 acggtagagg cgtccagcgg gttatcaccg tggttaatgc gcaggaagcc cgcgcactgc
     4681 tcgaaggctt tcggccccag acggctgact ttcagcagtt gctgacggtt cttgaactgg
     4741 ccgttctcat cgcgccaggc cacgatgttt tgcgccatca tgcgcgtcag gcccgccacg
     4801 cgagttaata gcggaacaga agcggtgttg agatcgacgc caacggcgtt tacgcagtct
     4861 tctactactg catccagttt gcgggccagt tgcgtctggc tgacgtcatg ctgatactga
     4921 cctacgccga tagatttcgg atcgattttc accagctccg ccagcggatc ctgcaaacgg
     4981 cgggcgatag ataccgcacc acgcagcgaa acgtcgagat ccgggaactc ctgcgctgcc
     5041 agctcggaag ctgagtaaac cgacgcgcca gcttcgctga cgatcacttt ctgtgcggtc
     5101 actttcggga actgcttctg cacgtcgaga tagaaacgtt cagtttcgcg ggaagctgta
     5161 ccgttgccga tcgccaccag ttcaacgtta tgtttttcac acaaggcagc aacggtcatc
     5221 gctacttttg cggcttgtcc ggtgtgcggg taaatggtat cggtcgccac cagtttgcca
     5281 gtggcatcga ccactgccac ttttaccccg gtacgcagac ccggatcgag gcccatcgtt
     5341 gcacgcagtc cggcaggggc cgccatcagc agatcgtgca ggttacgggc aaagacgttg
     5401 attgcttcat cttccgcgcg ttcgcgcacg gtacccatca gttcggtttc cagatgcatc
     5461 agcaccttga tgcgccaggt ccagctcact acgcctttgc gccagctatc cgccggggca
     5521 ttgttcaggc gcaggccaag gtgatccatg atgatttgct cgcaatagct ctctttgggc
     5581 ggctcatcga actgcggatc ggcattcagc gaaagctgga gtacgccttc gttacgccca
     5641 cggaacatcg ccagcgcgcg gtgagaaggc accgtggaca acggttcgtg atgatcgaaa
     5701 tagtcgcgga atttcgcccc ttcctcttct ttaccgctca ccaccgtaga aaccaaatgc
     5761 gcgttcttcc acagataatc acgcactttc gccagcagcg cggcatcttc ggcaaaccgt
     5821 tccatcagga tatagcgcgc gccgtccagc gcggctttgg tatctgccac gcctttatcg
     5881 gcatcaatat attgcgcagc ggcgacttct ggtgtgtgtg acggatcgct ccacagcagg
     5941 tcagccaacg gctcaagccc tgcttcaatg gcgatttgcc cgcgggtgcg gcgtttaggt
     6001 ttgtagggca ggtagaggtc ttcgagttcg gttttgctta gggtggcgtt gatggccttc
     6061 gccagatcat cggtgagttt gccttgctcg gaaatggact tgaggatcgc ctgacgtctc
     6121 tcttccagct cgcgcagata gctcagacgc gtttccagat tacgcagctg cgtgtcatcc
     6181 agaccgccgg tgatttcctt acgataacgt gcgataaacg gcacggtatt cccttcgtca
     6241 agcaggcgaa cggcagcgtc aacctgttcc gggcgcgcct gaatttcacc cgcaataatg
     6301 cggcagaacg aatcattcat catggtttga gttcatcttt tcggatcaaa aatcagggga
     6361 tagttatacg gactggctgg caaaaatgcc agccatcggc aggaggttaa gactcttcat
     6421 tacggtttca cgtattcgat agcattaaca taccagctcg cttccccggc aggggtattc
     6481 accaccgcca gatcgccgac ttcttttttc agcaatgcgc gggccatcgg ggaatcgata
     6541 gagatgtaat ctttacggcc aaaaatttca tcgtagccga caatacggaa acggtgagtc
     6601 acgccatcgt cgttttcaat ctccacccac gcgccaaaaa agactttgcc ttcctgctga
     6661 ggggagtaat tgacgatttt gagattttcc atgcatttag tgagatagcg cacgcgacgg
     6721 tcgatttcac gcagacgctt tttattatac tgatagtcag cattttcgct gcggtcgccc
     6781 agacttgcgg cccaggtcac cttttttgtg acctccgggc gttcttcacg ccagagataa
     6841 ttaagctctt gtttgagttt ttcataccct tcccgggtaa ccaggggcgt tttcatctcg
     6901 ttgattccct ttgtctgttt gataatgcgc acattgggta taacgtgatc atatcaacag
     6961 aatcaataat gtttcgccga ataaattgta tacttaagct gctgtttaat atgctttgta
     7021 acaatttagg ctgaaattca taccagattt agctggtgac gaacgtgagc ttttttaaga
     7081 atacacgctt acaaattgtt gcgaaccttt gggagtacaa acaatgcaag agaactacaa
     7141 gattctggtg gtcgatgacg acatgcgcct gcgtgcgctg ctggaacgtt atctcaccga
     7201 acaaggcttc caggttcgaa gcgtcgctaa tgcagaacag atggatcgcc tgctgactcg
     7261 tgaatctttc catcttatgg tactggattt aatgttacct ggtgaagatg gcttgtcgat
     7321 ttgccgacgt cttcgtagtc agagcaaccc gatgccgatc attatggtga cggcgaaagg
     7381 ggaagaagtg gaccgtatcg taggcctgga gattggcgct gacgactaca ttccaaaacc
     7441 gtttaacccg cgtgaactgc tggcccgtat tcgtgcggtg ctgcgtcgtc aggcgaacga
     7501 actgccaggc gcaccgtcac aggaagaggc ggtaattgct ttcggtaagt tcaaacttaa
     7561 cctcggtacg cgcgaaatgt tccgcgaaga tgagccgatg ccgctcacca gcggtgagtt
     7621 tgcggtactg aaggcactgg tcagccatcc gcgtgagccg ctctcccgcg ataagctgat
     7681 gaaccttgcc cgtggtcgtg aatattccgc aatggaacgc tccatcgacg tgcagatttc
     7741 gcgtctgcgc cgcatggtag aagaagatcc agcgcatccg cgttacattc agaccgtttg
     7801 gggcctgggc tacgtctttg taccggacgg ctctaaagca tgaggcgatt gcgcttctcg
     7861 ccacgaagtt catttgcccg tacgttattg ctcatcgtca ccttgctgtt cgccagcctg
     7921 gtgacgactt atctggtggt gctgaacttc gcgattttgc cgagcctcca gcagtttaat
     7981 aaagtcctcg cgtacgaagt gcgtatgttg atgaccgaca aactgcaact ggaggacggc
     8041 acgcagttgg ttgtgcctcc cgctttccgt cgggagatct accgtgagct ggggatctct
     8101 ctctactcca acgaggctgc cgaagaggca ggtctgcgtt gggcgcaaca ctatgaattc
     8161 ttaagccatc agatggcaca gcaactgggc ggcccgacgg aagtgcgcgt tgaggtcaac
     8221 aaaagttcgc ctgtcgtctg gctgaaaacc tggctgtcgc ccaatatctg ggtacgcgtg
     8281 ccgctgaccg aaattcatca gggcgatttc tctccgctgt tccgctatac gctggcgatt
     8341 atgctattgg cgataggcgg ggcgtggctg tttattcgta tccagaaccg accgttggtc
     8401 gatctcgaac atgctgcttt gcaggtcggt aaagggatta ttccgccgcc gctgcgtgag
     8461 tatggcgcgt ccgaggtgcg ttccgttacc cgcgccttta accatatggc ggctggtgtt
     8521 aagcaactgg cggatgaccg cacgctgctg atggcggggg taagtcacga cttgcgcacg
     8581 ccgctgacgc gtattcgcct ggcgactgag atgatgagcg agcaggatgg ctatctggca
     8641 gaatcgatca ataaagatat cgaagagtgc aacgccatca ttgagcagtt tatcgactac
     8701 ctgcgcaccg ggcaggagat gccgatggaa atggcggatc ttaatgcagt gctcggtgag
     8761 gtgattgctg ccgaaagtgg ctatgagcgg gaaattgaaa ccgcgcttta ccccggcagc
     8821 attgaagtga aaatgcaccc gctgtcgatc aaacgcgcgg tggcgaatat ggtggtcaac
     8881 gccgctcgtt atggcaatgg ctggatcaaa gtcagcagcg gaacggagcc gaatcgcgcc
     8941 tggttccagg tggaagatga cggtccggga attgcgccgg aacaacgtaa gcacctgttc
     9001 cagccgtttg tccgcggcga cagtgcgcgc accattagcg gcacgggatt agggctggca
     9061 attgtgcagc gtatcgtgga taaccataac gggatgctgg agcttggcac cagcgagcgg
     9121 ggcgggcttt ccattcgcgc ctggctgcca gtgccggtaa cgcgggcgca gggcacgaca
     9181 aaagaagggt aaataaacgg gaggcgaagg tgcctcccgt tttgctttct ataagatact
     9241 ggatagatat tctccagctt caaatcatta cagtttcgga ccagccgcta ccagcgcggc
     9301 acccgcaggg gtgtcggtgt atttatcgaa gttgtcgata aacagtttcg ccagggtttc
     9361 ggctttttcc tgccactgtt ccggagaagc gtaggtgtta cgcggatcga gaatcttcgt
     9421 gtctacgccc ggcagttcgg ttgggatcgc caggttaaac atcggcagag tgaaggtttc
     9481 tgcattatcc agcgaaccgt tgaggatggc gtcgataatg gcgcgggtat ctttaatcga
     9541 gatacgtttg ccagtgccgt tccagccagt gttaaccaga taagcctgcg cgcccgccgc
     9601 ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca ggaacgccgc
     9661 gccgaagcaa gcggagaagg ttggcgtcgg ttcggtgatg ccacgctcag taccggccag
     9721 tttggcggtg aagccagaga ggaagtgata ctgggtttga tcggcagtca ggcgagaaac
     9781 cggcggcaac acgccgaaag catcagcagt caggaagata accttagtcg cgtggcccgc
     9841 tttggaaacc ggcttaacga tgttatcgat gtgatagatt ggataagaaa cgcgggtgtt
     9901 ctcggttttt gaaccatcat caaagtcgat agtgccatct tcacgcacgg tgacgttttc
     9961 cagcagcgca tcacgacgga tagcgttgta gatttcaggt tccgcttctt tcgacagctt
    10021 gatagttttt gcgtagcagc cgccttcgaa attaaacacg ccgtcatcgt cccagccgtg
    10081 ttcgtcatcg ccaatcaggc ggcgtttcgg gtcggtggaa agggtggttt taccggtgcc
    10141 ggaaaggccg aagaacaccg caacatcgcc tttctcgcca acgttggcgg agcagtgcat
    10201 agaagcgata cctttcagcg gcagcaggta gttcatcatc gagaacatcc ctttcttcat
    10261 ttcgccgccg taccaggtgc cgccaatcag ctgcatgcgc tcggtcaggt taaacgccac
    10321 gaagttttcg gagttcagac cctgttcttt ccactgcggg ttagtgcact tcgcgccgtt
    10381 cataacgata aagtctagtt tgaaacctgc cagctcttca tcgctcgggc gaataaacat
    10441 gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg aaagacgagt
    10501 atccgggttc gcaccacaga aagcgtcgac aacgaacaga cgtttgccgg aaagctgcct
    10561 ggtcaccagg cctttcagat gctgccaggt ttccggagag agaggtttgt tgtcgttctt
    10621 acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac ggacgatata
    10681 cttatctttt ggtgaacgac cggtgaagat cccggtatcg acggcaacgg cacccagatt
    10741 agttaacacc ccgcgctcat aacctgtcag gctcggatcg agctcttcct gatacagcag
    10801 gtcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag cctcgagttc
    10861 ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat atgtattgcc
    10921 tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat ttacgtatct
    10981 ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg ttgctattat
    11041 cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct tggtttatat
    11101 ggcgggaata ttgtgagtgg aatcgcattc ctgaatgaaa ttgatgcaaa ttcaaccggt
    11161 aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag caaaatacta
    11221 ctgcccggat gcgataatta acccaccttt tgattctgga taaccaacat ggataacgtt
    11281 gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca ggggctggtt
    11341 tgctacctgc ttatcgcctg gcttgccgga aaaaatcaca gttggattgt ttatggcgtg
    11401 cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcttt taaacagaag
    11461 cgcctttggg gatggctggc gctggtgttt attgccacga cagggatgag cggttggctg
    11521 aagtggcaaa ctgatggcat gaacccctgg agagctgaaa aggcgatttg ggattttggc
    11581 tgatatctgc tgttgatggc aatgttgttg ctaccgtgga tacaacaaag ccttcgtatc
    11641 cgcaatggca gtagccgcta tagctacttt taccagtcag tatggcataa cgtacttata
    11701 ttactggtga tttttctctc caatggcctg acgtggctgg tgcttttact gtggggtgag
    11761 ttgtttaaac ttgttggcat cacgtttttc aatacgctct tttttgcaac cgactggttt
    11821 atgtatctta catttggtct ggttaccgca ctggtggtga tcctcgcgcg tacacagtca
    11881 cgtttaatcg actctattca aaagttgttc acgctaatcg ccacggggtt gctgccgtta
    11941 gtatcattgc taaccctgat gtttatcatc accctgccgt ttacggacct gagcgcgatt
    12001 tctcgccaca tctccgccgc cgggttgctg ttaacgctgg cctttttgca attgatctta
    12061 atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc gttgcgttgc
    12121 ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc ctgggcgtta
    12181 tggctgcggg tcgctcagta cggctggact gtcgaccgct tgcagggcgc gctggcggtg
    12241 ctggtgttac tggtgtggtc gctggggtat tttgtcagta tcgtctggcg taatgggcaa
    12301 aatccccttg ttcttcaggg caaagtgaac cttgcggttt cgttattggt gttggtgata
    12361 ctggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa cagtcatatg
    12421 gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat gctcgaacag
    12481 agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccgg gtttatgaaa
    12541 gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca tcttcaggaa
    12601 caggtatcgg aaaaagtatt agccgaaaat gtgttaattg ctcctggttc tgttaaaccg
    12661 gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac ctgtattgaa
    12721 aaagacgcct gcgtcctggt cgagcaagat ctgaatagtg atggtcaggc ggagcggatc
    12781 ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcaga cagaaaagaa
    12841 tgggacgcgc ttgatatgag tttacttccg aacgaaataa cgaaagaaaa attactcaca
    12901 gctgcgaagg atggaaaact ggatctgacg gtagatggtg aaacactgga aataaactta
    12961 agtaagtgac aactctttta acggatacga cgctgtatct gaccaacaat aatttgcgat
    13021 ttatttcaca ggcctggtaa gcgcagcaca tcaggccatt acgtcgtaaa atccggcaga
    13081 gccctctctg ccggacatac tcattaatga acttgcggat ctgccggaga cgcgttgttg
    13141 cggatttcag caatatccat cgcattgaac agatagtggt taccgcagta atcacaatgc
    13201 atgtcaattt cgccatcttc cgccaggatg ctatcgactt cttcatcagg cagcgttttc
    13261 agcgcgtcgg cgcaacgttc acgcgagcag gtgcatttga actccacatc ctgcggatcg
    13321 taaaccgtca cctcttcttc gtgatacaaa cgccacaaca cttcgtttgc cggtaaggtc
    13381 agcagttctt cggttttgat ggtttcggtt agcgtcgcca ggtggtcaaa gtcgtcctgc
    13441 tgggcatttt gcgcaggcat tacctgcaac aacataccgc ctgcagccgg tttgccgtct
    13501 acgtcgccgg tgcgaataaa caggcgcgtc ggcagctgtt cagaacgcat aaagtaatct
    13561 tccaggcagg ccgccagggt atcaccttcc agaccaacca cgccctgata gcgttcgcct
    13621 tcgctcgggg taatggtgat caccacgtaa ccattgccga ccagcgtttt caggtcggca
    13681 ttttctggaa tttcgccctg cacgcgcgcc acaccgcgca tttgctggtt attgttaccg
    13741 ttaataaccg ccagattcat cggaccgtcg ccttgcagct gtacggtgat atcaccatca
    13801 aacttcagcg tagcggttaa caggctggtc gcaaccagca gttctgccag cacgttttta
    13861 acgggctgcg gataatcgtg attctcaagg atctgttgca gggtttccga aacggttacc
    13921 agttcgccgc gcacggcaaa gttttcaaac agatagcgat gtaattggtc atgttgcggc
    13981 ataatcatct ctcttgcagg tgacagttat tcactgtcgc cgtgtttaaa tcgtaacagg
    14041 tcgcggcgct cttttttgtc cgggcgtcgg tccgggtgcg gcatggttaa ggcattaagt
    14101 ttacgtgcca gcgccatttt ttcgcgtttc tctacacttt ccgcagtctc ttcatacagc
    14161 aaggctgcct cgctggcggg gcgacgctgt tcagtaatcg cctttacaat caccgtgcgt
    14221 tcgtcatttc cctggcgcag agtgagcgtg gcattcagct cgacgatttt gctcggcttg
    14281 ctgcgctgcc cgttgtaatg caccttaccg ccttcaatca tttcacgggc cagcgcgcgg
    14341 gttttataaa aacgggcagc ccatagccat ttatccagtc gaacctcaac agcaggtttt
    14401 tctttcatgg cgtctccttc acattagcga ggggatcagg cggcggtagt cattcagtga
    14461 cggatggcgt tgatactgtt tctcggcaat cccggaatca ggattagtca cgccgaggca
    14521 gtaacgaata ccaaattgcg cggcagcatc gagaatcgct tcgctgtcat caataaacag
    14581 cgttctttca gctttcagac ccgtagcttc ggccaccgca tgccataacc gctgatcctc
    14641 tttcggataa ccaaatgtgt gggtggaaag taataaatca aggtgtgcgt ccagaccggt
    14701 atgctcaagt tttaccgcca ggttgtgcgg atgcgcattg gtgagcaaaa ttcgctgctt
    14761 accgctggct ttcagtgcct caagaaacgg aatggtatct tcacgcagta cggcacgcgg
    14821 tcccatctcg gtggtcatcg cacagatatc cagacccagt tgctcactcc agtaatcaag
    14881 acagtaccag tttagcgtat gctgtacgtc gtgatattgc tggcgcatat attccatcgc
    14941 ttcctgtggc gtaaccccgt ttttcgcgcc ccatgtttca ggcaccagct tttgccagaa
    15001 atagttatcg aaggcgaggt cgagcaacgt gccgtccata tccagcagaa cggtatctac
    15061 gtcctgccag gcaatgttga tatgcatgag ggaaatctcc agagtgaagc aatttgcgcg
    15121 acagggtagc ataacctgcc gcgcaaacgt gttattcgat aaggctttct gaaggggtga
    15181 tcagttgcgg gttcaggcag ctttcataat aggcctgaat cttcatcatg cgagtgcgat
    15241 gacgctggta acgacgccag gcctgtacgc cattgtaaat ggcgctaccc agcatcgtca
    15301 gtagcagcaa tgtggtgctg agatagcgcc acaggccgga acgatccggg attggatgaa
    15361 ggccaatatg ctgcgtacca ttggcgtcag tgaagatttt tgtgacgata ccctcggcgt
    15421 taaacggcgt atgcatcagc atttgtgcca gtttctggaa agcgttccac tgttcttgcg
    15481 gcgggtagtc gtaaagtgat gccgaaggcc agggctgatc aacaaaatcg ctgccttcgt
    15541 cgctgacaat caggaatccg ccgggggccg gactgtttag tgattgtgcc gctcgcgccg
    15601 tttcatgcgt gataaacggc gcggtggagg ttgccaccag gttatccagc gattccgcac
    15661 tcaccgggcg taataacaca ttcacgccat cgagcttccc ggcgttggcg cgttttacca
    15721 gcgcgtccca gtctttactg ttgccgagat tgaccagcgc atttttcagt cgcacacagt
    15781 catctttggc agaacataaa tccgctgtct tcagtacaat gtcgccaaaa tcatcaagca
    15841 ataccatgcc ggatttttga attgctgaac gtaatgaggc actgacgcga gatccatctt
    15901 ccggtttagg gtgcagctgg cgattaactg cttcagtcaa tgccgtcgct ttgttgacca
    15961 gttcagattc tggtaatggc aatgagcggg cgtcgttcca gatgatctgc gagcagtcaa
    16021 acggtaaaaa aggtgaattg gttttcgcgc tccaggttcc ggaagtgcga atattacaca
    16081 ttcccgtacc gctgatacgc aatgtatcgc ctacccgcac gccagcgtca gccagttgtt
    16141 ttacgctggt ggcttcaatg gtctgtgcgc ctttcatcca tgagagagtg aatttcagcg
    16201 gcatatccag cgggatccag aataacagca taaacagcac cagcagcgag cccgcagcga
    16261 taatcgtact gcgcagccag tgctgtaacg gaaagttttt tacttcatca tgcagcgaaa
    16321 gatatcgccc ctgacgcact acgtggcggt cgagatagat atcaatatcg gtttgttgac
    16381 cgagatcctg agcaatgtat ggctgccagt gtgcgggata gaccaggtcg ataataccga
    16441 gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa caatccccaa cgacggggtg
    16501 taccgcgcag acaatgaatt tcccgcaggg aggattttgc cgggggcgcg aatagccccc
    16561 acagacctgc gcccagcagc agtaatgcgc cgcctgccag ccacggaaca aatacatccg
    16621 gggtaatcag gcagaaaaag aacatcagga aggaggcgac gatcagcaac gcttcacgca
    16681 gcccgcgcgg acgactcagc gcatattctt catgcgtttc tttgcgaata ttgagtagct
    16741 cgatttgctc actctcttcc ccgcgaatcg acgcctgcgt ggacggaaca ggttgcaagg
    16801 catagccgcg cgtttcctgc atgtactcct gcagcgtatg accgttgagt gaaataacca
    16861 gcggcagcga atcggtataa atcagttcaa cggtattctc atcgttgatg tattgttccc
    16921 agaaaggggg caggtggact tctaccgaat cgaggtagta acgccattta ttcggatcgt
    16981 cggtagagat gccgtaacgc gtgatagcgt gtgtcagcat catgacgttg ttgctttcgg
    17041 cattcagcgc cagagagatc ggtgccgcgc tagctcccgt tgggccaggc acctgtaata
    17101 cctgcgtcag gctctcaaga taattttcaa cggcgctacg ttcttcaggt gtgagtttac
    17161 gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg acgccgtctg gatcgcactt
    17221 ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc agctaaaaaa atcacaatgg
    17281 tgctcatgct ttccccatct tacttagtct gtcaggcgtg gtcagtcgca ccagacacaa
    17341 tacatgattc tgcggttggc tatcggcaag cgtggagttt aacttgagca tcattaagca
    17401 catcacagta accgctgatc atagcaaacc ctctgaaggc gggatatcag caaattcctg
    17461 gagttatttc aacctattga cttttaattt gatttgagga caattattta ttaagttaca
    17521 taaatgtaaa taacatgcaa ttcacattgc cgaaagcgtg tccgatatcg cacaataacc
    17581 gctgatttca cagcatagat cccacgatga gcaaatcatt acaaaaaccc accattctga
    17641 atgttgaaac tgtagcccat tcccgactgt ttaccgtcga gagcgtggat ctggagttca
    17701 gcaatggcgt gcggcgtgtt tatgaacgaa tgcgtccaac caaccgggaa gcagtgatga
    17761 ttgtgccgat tgtggacgat cacctgatcc tgatccgcga atacgcagtg ggaactgaat
    17821 cctacgaatt aggtttttcg aaaggattaa ttgatccggg tgaaagcgtc tacgaagccg
    17881 ctaaccgcga gctaaaagaa gaggttggat ttggtgcgaa cgatctgact tttttgaaga
    17941 agctcagcat ggcaccgtct tacttttcca gcaaaatgaa tatcgtggtc gcgcaagatc
    18001 tctacccgga atcgctggaa ggcgatgagc cagagccgct accacaggtg cgctggccgc
    18061 tggcgcatat gatggatttg ctggaagacc ctgacttcaa cgaagcgcgt aatgtcagcg
    18121 cgctgttcct cgtgcgcgaa tggttgaaag ggcaggggcg agtgtaaatg cgtgaagttt
    18181 gcctgatgca caacgattat caggcttatg gggttcctgc aatatattga tttttcacga
    18241 ttttgttagc cggataacgc gttcacccgc atccggcata aacaaatgca ctttgtcagc
    18301 caactgaaaa ggcgccgaag cgccttttta atcagaacaa ttcctgtgcc tcaccattat
    18361 cgataatggt tgttcccacc tcgtgcaccg cctgttgtgt cggctgcgta ccttcgatga
    18421 aatactcttc gcggctgttg ccaccattag ctaactgccc ggtgctgcga tcgatattca
    18481 ccgtcacaat acccggaggc ggcgtcagcg gctgctccgg cacaccttca agaacggctt
    18541 tcatataagc gtcccatgca ggctgggcac tcttggcacc gccttcgtaa cctgagatct
    18601 gatctttaat cgctccggaa gccgttgtat gaccgagatt acgacggtga tcatcaaagc
    18661 caatccagac cgaggtcaca acgcccggac cgtaacccga gaaccacgca tctttcgaac
    18721 tgttagtggt accggttttc ccgccgatat cgcgacgctg caaatcacga cctgcacgcc
    18781 agccggtacc ctgccagcct ggttcaccaa agatattggt gttcaaagca ctcttaatca
    18841 ggaatgccag cggagtgttg atgacgtgcg gtgcgtactc ctgcgcgcca gtcttcgcca
    18901 ctaacgcctg atttgcctgc tccagctgcg gcattggtac agaaacattc tgctgctcgc
    18961 gggagatagc gacatcttca acatcgttat tttccagcac gttcgatttc tgcgtatcac
    19021 cgtaaatcac cggaatatcg cattccgggc aggctacttt cggtttcgct tcgaaaatca
    19081 cgccgccctg atcgttttca attttgctga taaaccacgg gtccaccagg aagccgccgt
    19141 tcgccatgac cgcgtagccg cgcgccacct gcattggggt gaaggacgct gaacccagcg
    19201 ccagcgattc ggtgtggaca atgttttgcg ccgggaagcc gaagcgttgc agatattctg
    19261 cagcgtagtc gacgcccatc gcccgcattg cacgtaccat caccacgttt ttcgactgac
    19321 ccagcccctg acgtaagcga attggaccag catactgcgg tggtgagttc ttcggctgcc
    19381 agtcagaacc gacacctgca tcccagcgag aaattggcac atcgttcaac atacttgcca
    19441 gcgtcagacc tttatccatc gccgcggtgt agaggaacgg tttgatgttg gaacccacct
    19501 gacgcagtgc ctgggtggcg cggttaaact tgctctgatt gaaatcaaag ccaccgacca
    19561 gcgccataac ggcaccattt tgcggattga tcgacaccag cgccgagttc acttccggca
    19621 cttgtgccag ccaccatgca tcgccaacct gacgaaccca gatttgctga cccgtttgca
    19681 gaacatcggt cactttacgc ggcgtcggtc cctgctgagt atccgaacgg taaggacgcg
    19741 cccagcgaac gccgtccata ctcaatgcga cagtcgaccc gtccgccagc attgccgtcg
    19801 cttcctgagg attggcgctg gtgactgcgg caggcagcag cggaccatag gtcggcagcg
    19861 ccttcagcgt atcggtaatc ttgttgttat cccacgccga ctcgcccact ttccacagca
    19921 catttgccgg gccgcgatag ccgtggcgca tgtcgtagtc cagcacgtta ttacgtaccg
    19981 cctgctgtgc ggcctgctgc actttgcggg tgatggtggt gtaaatgcga taaccgtctt
    20041 cataggcact ttcgccataa cggttataca tctcctggcg caccatttcg ctcaggtacg
    20101 gcgcagagaa agcaatctcc ggcgcgtgat agttagcgtt aatcgcctca gtgcgtgtct
    20161 gatcgaactg ttgttgggtg atataccctt catccagcat ccgcgacagt acgacgttac
    20221 gccgcgcgac ggcacgatcc attgagtaga gcggattaaa ggtggaaggc gctttcggca
    20281 gcccggctat cacagccatt tcgttcagcg tcagttggtc gaccgtttta ccgaaataga
    20341 cttgtgccgc agcaccgaca ccataggcgc ggtaaccgag gtaaatcttg ttcagataaa
    20401 gctcgaggat ctcgtctttc gtcagcaact gttcaatgcg aatcgcgagg aagacttcct
    20461 taatcttacg catcagcgtg cgttctggac tgaggaagaa gtttctcgcc agctgctggg
    20521 taatggtact cgccccttgt gacgcgtggc cggagaacag cgccacactt gccgcacgga
    20581 agatccccac cgggtcaacg ccgtgatgct cgtagaagcg gctgtcttcc gtcgcgataa
    20641 aggctttcac catctccggc gggatttgat ccaacgtaac cggaatacga cgtttctcac
    20701 cgtattgagc aatcagctcg ccatcggcgc tgtaaatctg catcggaatt tgcaggcgaa
    20761 catcttttaa tgtcgccaca tccggcagtt gtggctcgat gtagcggtat aggccataaa
    20821 tcgagcctgc tcccagcaga atgcaacaga ctgcaaggat caaaaaatac tttacgaact
    20881 tcactggaaa tttcccattt agtttcattt gggcagttta taaacaaacg tgcggtagta
    20941 taaaggcaag ccagacgcat tgatataccc gtcagagtga cgggtgataa ggagattatc
    21001 acaatggcat ttaagatctg gcaaattggt ttgcatttac aacagcaaga agcagtagcg
    21061 gttgcgatcg tgcggggcgc aaaagaatgc tttttgcaac gctggtggcg gttgccgctg
    21121 gagaacgaca ttatcaaaga tgggcggatt gttgatgcgc agcggctggc taaaacgttg
    21181 ttaccgtgga gtcgcgaact gccgcagcgt catcacatta tgttggcgtt tcccgccagt
    21241 cgcacattac agcggtcatt tccgcgcccg tcgatgtccc tcggtgagcg ggagcaaacg
    21301 gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc cctgcgtttc
    21361 gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca aagcaaagag
    21421 ctggcaacgc tactcactct ggcagaaagg ttgcgtgttc atgtgagcgc gatcaccccg
    21481 gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca atgtctggcc
    21541 tggcgtgata acgaacagtg gctgtgggcg acacgctgtc gctgggggcg caaactggcg
    21601 gtggggatga ctagcgcgaa ggagctggcg gcagcgttat ccgttgatcc cgaaagcgtc
    21661 gcgatatgtg gcgaaggcgg atttgatccc tgggaggccg tttctgttcg tcagccgccg
    21721 ctaccgccgc caggtggaga ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac
    21781 tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc gcttttcttc
    21841 gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata acgctaatac
    21901 tgcgtctgac agacaacgcc gaagcacgcg taaacaccgt tttgcttcag gcggaacaac
    21961 aactcgcccg cagcttacag ataacgaaac cacgtttgct ggagcgccaa caaattcgtg
    22021 agcagcgttt gcaacggcag cgccagcgac aatttacccg cgactggcaa tctgcgctgg
    22081 aagcactggc ggcgctttta cccgagcacg cctggctgac aacgataagc tggcagcagg
    22141 gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt
    22201 cactccgcca ggatgcgtct tttcatctca atcagcgggg agccacgcag caggatgcgc
    22261 agggacgctg gcaatttgag tatcagttaa caaggaaggt tagcgatgaa catgttcttt
    22321 gactggtggt tcgccacatc accccgcctc cgccagtttt gctgggcatt ctggttgctg
    22381 atgttagtta cgctcatttt tctgtcatcg acacaccatg aagagcgcga cgcattaatt
    22441 cgactacggg caagtcatca ccagcagtgg gccacactgt atcgcctggt tgacaccact
    22501 cccttcagcg aagaaaaaac gctgcccttt tcgccactgg attttcagtt gcccggcgcg
    22561 caactggttt cctggcatcc atccgcgcag ggaggcgagt tggcgttgaa aacgctgtgg
    22621 gaagcagtgc cgtcggcatt tacacggctg gcagagcgca acgtcagcgt gagccgtttt
    22681 tcattaagcg tggaaggtga tgatcttttg ttcacgctac aactggagac gccgcatgag
    22741 ggttaaacgc tggttgttgg caggtattgt attgtgcctt ttaaccggta tgcgtgaccc
    22801 ttttaaaccg ccggaagatc tatgccggat tagcgaactt agccagtggc gctatcaggg
    22861 gatggtaggg cgaggcgagc gcatcatcgg tgtaataaaa gacgggcaaa agaaatggcg
    22921 acgggtgcag caaaacgatg tgctggaaaa cggctggaca attttacagc tgacgccaga
    22981 cgtactaacg ctgggtaccg ggacaaactg cgaaccgcca caatggttgt ggcaacggca
    23041 aggagataca aatgaagcaa tggatagccg cactactgtt gatgctgata cccggcgtac
    23101 aggcggcaaa gccgcaaaaa gtgacgctga tggtggatga cgttccggta gctcaggtgt
    23161 tgcaggcgct ggctgaacag gagaagttga acctggtcgt gtcgccagac gtcagcggta
    23221 cggtgtcgtt acatctaaca gatgttccct ggaagcaggc actacaaact gtagtgaaaa
    23281 gcgccggact gataacgcgg caggaaggca acattctctc agtgcattcc attgcctggc
    23341 agaataacaa tatcgcccgc caggaggcgg agcaggcgcg ggcgcaggca aatctgccgc
    23401 tggaaaatcg cagtataacc ctgcaatacg ccgacgcggg agaactggcg aaagcggggg
    23461 agaagctact gagtgccaaa gggagtatga ccgtcgataa acgcaccaat cgccttttgc
    23521 tacgagataa caaaacggcg ttaagcgcgc ttgaacagtg ggtagcgcaa atggatctgc
    23581 cggtcgggca ggttgagctg tcggcgcata ttgtcaccat taatgaaaaa agtttgcgtg
    23641 agttaggcgt gaaatggacg ctggccgatg cgcaacacgc tggtggcgtt gggcaagtca
    23701 ccacgcttgg tagcgacctc tccgtagcga cggcgacaac gcatgtcggt tttaacattg
    23761 ggcgcatcaa cggacgcttg ctggatcttg agctttccgc gctcgaacaa aaacagcagc
    23821 tggatattat cgccagtccg cgtctgctgg cctcacatct tcagcctgcc agcattaaac
    23881 aggggagcga aattccatat caggtttcca gcggggaaag tggcgcgacg tcggtggaat
    23941 ttaaagaggc cgtcctgggg atggaagtta cgcccacagt gttacaaaaa ggtcgcatcc
    24001 ggctgaaatt acacatcagc cagaacgttc cggggcaggt gctacagcag gccgatggcg
    24061 aagtgctggc gattgataag caggagatcg aaacgcaggt cgaggtcaaa agcggagaaa
    24121 cgttggcgct gggcggcatt tttacccgta aaaataaatc gggtcaggat agcgtaccgt
    24181 tgcttggcga cattccctgg ttcgggcaat tatttcgtca tgacggaaaa gaagatgaac
    24241 gacgcgagtt agtggtgttt atcacgccac gactggtttc cagtgagtaa acagccgtaa
    24301 aagcggtaat gtttttacgc tgaacgtgtt tcatctattt gacgcgcgca ggtatttagc
    24361 atacaaggag taccgatttg agagttggtg ctcttcgctg cctgcgttcc atgatgatga
    24421 tttatcattc aggcggcatt ttgctgtctt ttttacgcta atcttacccg gtgatttatc
    24481 gccagagcgg tggtagcaag gcagcgcgct tgcagcgacc agatatgcag agggatgggt
    24541 gatttattca gttgccaaac ccgctggagt attgagataa ttttcagtct gactctcgca
    24601 atatcttatg aggtttcagt tcatgtcctg cggcgctctc tgagcgaagc gggtttatca
    24661 ttaacgaata gtcttagtag taccgaaaaa atggcagaga aacgcaatat ctttctggtt
    24721 gggcctatgg gtgccggaaa aagcactatt gggcgccagt tagctcaaca actcaatatg
    24781 gaattttacg attccgatca agagattgag aaacgaaccg gagctgatgt gggctgggtt
    24841 ttcgatttag aaggcgaaga aggcttccgc gatcgcgaag aaaaggtcat caatgagttg
    24901 accgagaaac agggtattgt gctggctact ggcggtggct ctgtgaaatc ccgtgaaacg
    24961 cgtaaccgtc tttccgctcg tggcgttgtc gtttatcttg aaacgaccat cgaaaagcaa
    25021 cttgcacgca cgcagcgtga taaaaaacgc ccgttgctgc acgttgaaac accgccacgt
    25081 gaagttctgg aagcgttggc caatgaacgc aatccgctgt atgaagagat tgccgacgtg
    25141 accattcgta ctgatgatca aagcgctaaa gtggttgcaa accagattat tcacatgctg
    25201 gaaagcaact aattctggct ttatatacac tcgtctgcgg gtacagtaat taaggtggat
    25261 gtcgcgttat ggagaggatt gttgttactc tcggggaacg tagttaccca attaccatcg
    25321 catctggttt gtttaatgaa ccagcttcat tcttaccgct gaaatcgggc gagcaggtca
    25381 tgttggtcac caacgaaacc ctggctcctc tgtatctcga taaggtccgc ggcgtacttg
    25441 aacaggcggg tgttaatgtc gatagcgtta tcctccctga cggcgagcag tataaaagcc
    25501 tggctgtact cgataccgtc tttacggcgt tgttacaaaa gccgcatggt cgcgatacta
    25561 cgctggtggc gcttggcggc ggcgtagtgg gcgatctgac cggcttcgcg gcggcgagtt
    25621 atcagcgcgg tgttcgtttc attcaagtcc cgacgacgtt actgtcgcag gtcgattcct
    25681 ccgttggcgg caaaactgcg gtcaaccatc ccctcggtaa aaacatgatt ggcgcgttct
    25741 accagcctgc ttcagtggtg gtggatctcg actgtctgaa aacgcttccc ccgcgtgagt
    25801 tagcgtcggg gctggcagaa gtcatcaaat acggcattat tcttgacggt gcgtttttta
    25861 actggctgga agagaatctg gatgcgttgt tgcgtctgga cggtccggca atggcgtact
    25921 gtattcgccg ttgttgtgaa ctgaaggcag aagttgtcgc cgccgacgag cgcgaaaccg
    25981 ggttacgtgc tttactgaat ctgggacaca cctttggtca tgccattgaa gctgaaatgg
    26041 ggtatggcaa ttggttacat ggtgaagcgg tcgctgcggg tatggtgatg gcggcgcgga
    26101 cgtcggaacg tctcgggcag tttagttctg ccgaaacgca gcgtattata accctgctca
    26161 agcgggctgg gttaccggtc aatgggccgc gcgaaatgtc cgcgcaggcg tatttaccgc
    26221 atatgctgcg tgacaagaaa gtccttgcgg gagagatacg cttaattctt ccgttggcaa
    26281 ttggtaagag tgaagttcgc agcggcgttt cgcacgagct tgttcttaac gccattgccg
    26341 attgtcaatc agcgtaacaa caagaaaggt caggccgctt atcaagcggt ctattagctt
    26401 caggttaatt gcaacgtggt aagcattaac cttttagtgg ggtgttaaat ggatgaattc
    26461 aaaccagaag acgagctgaa acccgatccc agcgatcgtc gtactggtcg ttctcgtcaa
    26521 tcttctgaac gttctgagcg tactgaacgt ggcgaaccgc agatcaattt tgatgatatt
    26581 gaacttgatg acactgacga tcgccgtccg actcgtgcgc aaaaagagcg caatgaggaa
    26641 ccggaaatcg aagaagaaat tgacgaatcc gaagatgaaa ccgtggatga agagcgcgta
    26701 gagcgtcgtc cgcgtaagcg caaaaaagca gccagtaaac ccgcttctcg tcagtatatg
    26761 atgatgggcg tcggcattct ggttctactg ctgttgatca tcggtatcgg ttctgcgcta
    26821 aaagccccct cgacctcttc cagcgatcaa accgcgtctg gcgagaagag tattgatctt
    26881 gcaggcaatg cgaccgatca ggcgaatggg gtgcagccag cgccgggaac cacgtctgcg
    26941 gaaaatactc agcaggatgt ttctctgcca ccgatctctt ctacgccgac tcaagggcaa
    27001 accccggcgg caacggatgg tcaacaacgt gttgaagtgc agggtgacct gaacaatgcg
    27061 ctgacccagc cacaaaatca gcaacagttg aacaatgtgg cggtcaattc cacattgccg
    27121 accgaacccg cgactgtcgc gcctgttcgc aatggcaatg catcgcgtga cacggcgaaa
    27181 acgcaaaccg ctgaacgtcc gtccactacg cgcccagctc gtcagcaggc ggtgattgaa
    27241 ccgaaaaaac cgcaagcaac cgtgaaaacg gagccgaagc cggtagcaca gacgccgaag
    27301 cgtactgaac cagctgcccc tgtggcgagc acgaaggcac cgactgcgac ttctacgcca
    27361 gcaccaaaag agacggcgac tacggctcca gtacagacgg catccccggc gcaaaccacg
    27421 gcaacaccag ccgctggagg gaagaccgca ggtaatgttg gttcgttgaa atcggcaccg
    27481 tccagccatt acactctgca gctgagcagt tcctctaact acgacaacct gaacggttgg
    27541 gcgaagaaag agaatctgaa aaactacgtt gtctatgaaa cggcgcgtaa tggtcagccg
    27601 tggtatgtcc tggtttctgg cgtgtacgct tcgaaagaag aggcgaaaaa agcggtatct
    27661 acattgccag cagatgttca ggccaaaaac ccgtgggcga aaccgctgcg tcaggtacag
    27721 gccgatctga agtaatcaag gttatctccc gcaatggttt atcgttgcgg gagctgcctg
    27781 aagcgctgga tgctgtcgga gctttctcca cagccggaga aggtgtaatt agttagtcag
    27841 catgaagaaa aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga
    27901 tattaaacgg catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc
    27961 ggtgtttctc aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat
    28021 cagtctctat aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct
    28081 gtttgttccc gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa
    28141 aagccaggat ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa
    28201 cggcctgtgt cgttacaatc tgcgcggtga gtttaacgtg ccgtttggcc gctacaaaaa
    28261 aacctatttc ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt
    28321 ctattgtgag tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg
    28381 cgatccgcct tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag
    28441 ttttacgctt gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca
    28501 tattccagtg ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc
    28561 aaaattgcat gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa
    28621 ggtggacgaa ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataatt
    28681 ctcaaggaga agcggatgaa acagtatttg attgccccct caattctgtc ggctgatttt
    28741 gcccgcctgg gtgaagatac cgcaaaagcc ctggcagctg gcgctgatgt cgtgcatttt
    28801 gacgtcatgg ataaccacta tgttcccaat ctgacgattg ggccaatggt gctgaaatcc
    28861 ttgcgtaact atggcattac cgcccctatc gacgtacatc tgatggtgaa acccgtcgat
    28921 cgcattgtgc ctgatttcgc tgccgctggt gccagcatca ttacctttca tccagaagcc
    28981 tccgagcatg ttgaccgcac gctgcaactg attaaagaaa atggctgtaa agcgggtctg
    29041 gtatttaacc cggcgacacc tctgagctat ctggattacg tgatggataa gctggatgtg
    29101 atcctgctga tgtccgtcaa tcctggtttc ggcggtcagt ctttcattcc tcaaacactg
    29161 gataaactgc gcgaagtacg tcgccgtatc gacgagtctg gctttgacat tcgtctggaa
    29221 gtggacggtg gcgtgaaggt gaacaacatt ggcgaaatcg ctgcggcggg cgcggatatg
    29281 ttcgtcgccg gttcggcaat cttcgaccag ccagactaca aaaaagtcat tgatgaaatg
    29341 cgcagtgaac tggcaaaggt aagtcatgaa taagtttgaa gatattcgcg gcgtcgcttt
    29401 tgatcttgat ggtacgctgg tcgacagtgc tcccggtctt gctgctgcgg tagatatggc
    29461 gctgtatgcg ctggagttgc ccatcgcagg tgaagaacgc gttattacct ggattggtaa
    29521 cggcgcagat gttctgatgg agcgcgcatt gacctgggcg cgtcaggaac gtgcgactca
    29581 gcgtaaaaca atgggtaaac cgcccgttga tgacgacatt ccggcagaag aacaggtacg
    29641 tattctgcgt aaactgttcg atcgctacta tggcgaggtt gccgaagagg ggacgttttt
    29701 gttcccgcac gttgccgata cgttgggcgc gttgcaggct aaaggcctgc cgctaggcct
    29761 ggtcaccaac aaaccgacgc cgttcgtcgc accgctgctc gaagccttag atattgccaa
    29821 atactttagc gtggttatcg gcggcgatga tgtgcaaaac aaaaaaccgc atccggaccc
    29881 gctgttactg gtggctgagc ggatgggaat tgccccacaa cagatgctgt ttgtcggcga
    29941 ctcacgcaat gatattcagg cggcaaaagc ggcaggttgc ccatcagttg gcttaaccta
    30001 cggatataac tacggcgagg ctatcgatct cagccagcct gatgtaattt atcagtctat
    30061 aaatgacctt ctgcccgcat tagggcttcc gcatagcgaa aatcaggaat cgaaaaatga
    30121 ctaagcccat cgtttttagt ggcgcacagc cctcaggtga attgaccatt ggtaactaca
    30181 tgggtgcgct gcgtcagtgg gtaaacatgc aggatgacta ccattgcatt tactgtatcg
    30241 ttgaccaaca cgcgatcacc gtgcgccagg atgcacagaa gctgcgtaaa gcgacgctgg
    30301 atacgctggc cttgtatctg gcttgtggta tcgatcctga gaaaagcacc atttttgttc
    30361 agtcccacgt accagaacat gcgcagttag gctgggcact gaactgttat acctacttcg
    30421 gcgaactgag tcgcatgacg cagtttaaag ataaatctgc gcgttatgcc gagaacatca
    30481 acgctggtct gtttgactat ccggtgctga tggctgcgga catcctgctg tatcaaacta
    30541 atctggtacc ggtgggtgaa gaccagaaac agcacctgga actgagtcgc gatatcgccc
    30601 agcgtttcaa cgcgctgtat ggcgagatct ttaaggtgcc ggagccgttt attccgaaat
    30661 ctggcgcgcg cgtaatgtcg ctgctggagc cgaccaagaa gatgtccaag tctgacgata
    30721 accgcaataa cgttatcggc ctgctggaag atccgaaatc ggtagtgaag aaaatcaaac
    30781 gcgcggtcac tgactccgac gagccgccgg tagttcgcta cgatgtgcag aacaaagcgg
    30841 gcgtttccaa cctgctggat atcctttctg cggtaacggg ccagagcatc ccagaactgg
    30901 aaaaacagtt cgaaggcaag atgtatggtc atctgaaagg tgaagtggct gatgccgttt
    30961 ccggtatgct gactgaattg caggaacgct atcaccgttt ccgcaacgat gaagccttcc
    31021 tgcaacaggt gatgaaagac ggcgcagaaa aagccagtgt acacgcttcc cgtacgctga
    31081 aagcggtgta cgaagcgatt ggttttgtgg cgaagccgta atttaacatt aatgccaaaa
    31141 accggggaaa cccggttttt ttatcctcat ttgcaataat ccgaaaaaat gtgaagcgcc
    31201 tcgccgtttc cacatctcgt cgttgccact tatcttcatt ttaatgaaga tataaatatt
    31261 cattttattg aaaatatatt atgcgtcgaa cgtttatcaa aaaagaaggc gtcgtcatca
    31321 tgacgctggc ccgttatttg ttgggtgaaa aatgcggtaa tcgactgaaa accatagatg
    31381 agctggcaac tgaatgccgt tcatcggttg gcctgacgca ggccgcgttg aaaacgttgg
    31441 aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa tggcagttat ctggtcgaga
    31501 tggataacaa agcattgctg agtcatgtgg atatcaacaa cgtggtatgt gcgatgcctt
    31561 tgccctatac ccgcttgtac gaaggtttag cgagcgggct gaaagctcag tttgatggca
    31621 ttccttttta ctatgcgcat atgcgcggcg cggatattcg cgtggagtgt ttacttaacg
    31681 gcgtgtatga catggcggtg gtttcccgac tggcggcgga aagttatctc acgcaaaaag
    31741 ggttatgcct cgcgctggag ttggggccgc acacctacgt tggcgagcac cagttgattt
    31801 gccgtaaagg cgagtccgca aacgtgaagc gcgtggggct ggataaccgt tcggcggatc
    31861 agaaaatcat gaccgatgtc ttttttggcg atagtgatgt ggaacgtgtc gatctctctt
    31921 atcacgagag tttacaacgc attgttaaag gcgatgtcga tgcggttatc tggaacgtgg
    31981 tggcggaaaa cgaactgacc atgctgggat tagaggcgac gccgctcaca gacgatccgc
    32041 gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt cgatgattac ccaatgcaac
    32101 aactgctgcg tgccgttgta gataaacacg ccctgctcgc ccatcaacaa cgggtagtga
    32161 gtggggaaca ggaaccgagt tattaaacga aaggcatctg atatggaaac cagactcaac
    32221 ctgctttgcg aggcaggcgt tattgataag gacatctgca aaggcatgat gcaggtcgtc
    32281 aacgtactgg aaacagagtg ccatctgccg gtgcgcagtg agcaaggaac gatggcgatg
    32341 acacatatgg cgagtgcact gatgcgcagt cgccgtggtg aagaaataga gccgctggat
    32401 aacgaattgc tggcagagct ggcgcaatcc agccactggc aagccgttgt gcaattgcat
    32461 caggtgttgt tgaaggaatt cgcactggaa gttaacccgt gtgaagaagg ctatttgctg
    32521 gccaaccttt atggattatg gatggctgct aacgaagagg tttgaagctc ccgacaaatc
    32581 cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta attcaaggaa cagaaacgag
    32641 gcaacaagat gtttgtagaa gcattgaaac gccagaaccc ggcgctgatt tccgccgcac
    32701 taagtttgtg gcagcagggc aagatcgccc cggacagctg ggtgatcgac gtggatcagg
    32761 tactggaaaa cggtaagcga ctgattgaga cggcgcggct ttacggcatt gaactgtatc
    32821 tgatgaccaa gcagtttggc cgtaacccat ggctggcgga aaaattgctg gcattaggtt
    32881 acagcggcat tgtggcggtg gattacaaag aggcgcgagt catgcgccgc gctggtttgt
    32941 cggtggcaca tcaggggcat ctggtacaaa tcccttgtca tcaggttgct gacgccgttg
    33001 aacagggcac cgacgtcatc accgtgttta ctctcgacaa agcgcgggaa gtttctgcgg
    33061 cagcggtgaa ggccggacgt gtgcagtttg tgctgctcaa agtttatagc gacgatgatt
    33121 ttctttatcc ggggcaggag agcggttttg tacagcattc gctgcacgag gtggttgctg
    33181 aaattaagaa actgccgggg ctgcatttag ccggacttac ccatttccct tgcctgcttt
    33241 gggatgaggc tgccggaaaa gttttgccga caccgaatct tcacacgctg atacaggcac
    33301 gggatcaact ggcgaaatct ggtattgcac ttgagcaact gaacgcgcct tcagcgacca
    33361 gctgcacttc gctgccatta ctggcgtaat acggtgtgac tcatgccgaa cccggtcatg
    33421 cgctgacggg cactattccg gcaaaccagc agggcgatca gtctgaacgt atcgcgatgc
    33481 tctggttgag tgaaatctcc catcatttcc gtggcgacag ctactgctac ggcggcggtt
    33541 actaccgtcg tggtcatgcg caacatgcgt tggtgtttac gccagaaaat caaaagatta
    33601 ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga ctacaccctg tcgctggcag
    33661 gcgagtttcc ggtaagcagt gcagtggtgc tctgtttccg cacgcagatt tttgtcaccc
    33721 gtagcgatgt ggtgctggtg tccggtattc accgtggcga accgaaaata gttggtcgtt
    33781 atgacagtct tggaaactct ctgggggcgt aatggcgcga tttgtggtgt tagtgattga
    33841 tagctttggc gtaggggcaa tgaaagatgt cacgctggtg cgtccgcaag atgcgggagc
    33901 gaatacatgt ggtcacatcc tgagccagtt gccgcatttg cagctaccaa cgctggagaa
    33961 gctggggcta atcaacgcat tgggttatgc gccaggcgat atgcagccgt cagattccgc
    34021 aacctggggc gtggcagagc tgcaacatga aggtggcgat acctttatgg ggcatcagga
    34081 aattttaggc acgcgcccgt taccgccgct gcggatgcct tttcgcgatg tgattgaccg
    34141 tgttgagcag gcattagtat ccgccggttg gcaggtggag cgccgtggcg atgaactgta
    34201 atttctgtgg gtcaatcagg cggttgcgat tggcgataat ctcgaggcgg atttaggcca
    34261 agtctataac attaccgcca atctctctgt gatctctttt gacgacgcaa tcaaaattgg
    34321 tcgtatcgtg cgtgagcagg tacaggtcgg tcgggtcatt acatttggtg gcctgttaac
    34381 cgacagtcaa cgcattctcg atgccgcaga aagcaaagaa gggcgcttta ttggtatcaa
    34441 tgcgccgcgt tctggcgctt atgacaacgg cttccaggtc gtgcatatgg gctacggtgt
    34501 cgataaaaaa gtgcaggtgc cgcaaaaact gtatgaggcg ggcgtgccaa ccgtgctggt
    34561 gggtaaggtg gcagatatcg tcagcaatcc ttatggcgtg agctggcaaa acctggtgga
    34621 tagccagcgg attatggata tcaccctcga cgaatttaac acccatccga cggcgtttat
    34681 ttgcaccaat atccaggaaa ccgacctcgc tggtcatgca gaagacgtcg cacgttatgc
    34741 cgaacgtttg caggtcgttg accgtaacct tgcccggctt gttgatgcga tgcagccaga
    34801 tgattgcctg gtcgtgatgg cggatcacgg caacgatccg accattggtc acagccacca
    34861 tacccgcgaa gtggtgccgc tgctggttta tcagcaaggc ctggctgcta cgcagctcgg
    34921 tgtgcgcacc acgctttctg atgtgggagc taccgtgtgt gaatttttcc gtgcgccacc
    34981 gccacaaaat ggtcgctctt ttctttcctc cctccggttt gcaggagaca ccctatgagt
    35041 tttgatccga cgggttacac cctggcccat gagcatctgc atattgatct ctccggcttt
    35101 aaaaacaacg tggactgccg ccttgatcag tatgcgttca tttgccagga gatgaacgac
    35161 ctgatgaccc ggggtgtacg taatgtgatt gagatgacca accgttacat ggggcgcaat
    35221 gcgcaattta tgctcggtgt aatgcgcgaa accgggatca acgtggtggc ctgtactggt
    35281 tattaccagg atgcgttttt cccggaacat gtggtgaccc gcagcgtgca ggaactggcg
    35341 caggagatgg tcgatgaaat tgaacagggt atcgatggca cggagctgaa agccgggatc
    35401 atcgcggaga tcggctccag cgaaggaaag attacgccgc tggaagagaa ggtatttatt
    35461 gccgctgcgc tggcgcataa ccagaccgga cgcccgatct ccacgcatac gtcgttcagc
    35521 accatggggc tggaacaact ggctctgttg caagcccacg gagttgatct ttcgcgcgtc
    35581 accgttggtc actgcgatct gaaagacaac ctcgacaaca ttttgaagat gatcgatctc
    35641 ggcgcgtacg tgcagttcga caccattggt aagaacagtt actacccgga cgaaaagcgc
    35701 attgcgatgc tccacgcgct acgtgaccgt gggttgctga accgcgtcat gctgtcgatg
    35761 gatattacgc gccgctccca tttaaaagcc aatggtggtt atggctatga ctatttattg
    35821 accaccttta ttccgcaatt gcgccagtca ggattcagtc aggccgatgt ggatgtgatg
    35881 ttacgtgaaa ctccctctca atttttccaa taaggacaga ctcatgaaaa agattggcgt
    35941 tgcaggctta cagcgtgagc agattaaaaa aaccattgaa gcgacggctc ctggcagttt
    36001 tgaagttttc attcacaacg acatggaagc ggcaatgaaa gtgaaatccg ggcaactgga
    36061 ttactacatc ggtgcgtgta ataccggtgc gggcgcggca ttgtcgattg ccatcgcggt
    36121 gattggctat aacaaaagtt gcaccattgc caaaccaggc attaaagcga aagacgagca
    36181 tatcgccaaa atgatcgctg aaggaaaagt ggcgtttggc ctttccgttg agcacgtcga
    36241 acacgcgatt ccgatgctga ttaaccatct gaaataaaag gcacgactat ggatctgtat
    36301 attcagatta tcgtggtggc gtgcctgacg ggtatgacat cgcttctggc gcatcgctcg
    36361 gcggctgttt ttcatgacgg tatccgcccg atcctgccgc aactgattga aggctatatg
    36421 aaccgtcgcg aggcggggag tatcgctttt ggtctgagca ttggttttgt ggcctcggtg
    36481 gggatctctt ttaccctgaa aaccgggctg ctcaacgcat ggttactctt tcttcctacc
    36541 gatatcctcg gcgtactggc gataaacagc ctgatggcgt ttggtcttgg cgctatctgg
    36601 ggcgtgttga tccttacttg cctgttgcca gtaaaccagc tgctgaccgc gctgccggtg
    36661 gatgtattag gtagcctcgg ggaattaagc tcgccggtgg tttctgcttt tgcactgttc
    36721 ccgctggtgg cgattttcta ccagtttggc tggaagcaaa gtctggtcgc cgccgtggtt
    36781 gttctgatga cccgtgtggt agtcgtgcgc tatttcccac atcttaaccc tgaatccatc
    36841 gaaatcttta ttggcatggt gatgctgctg gggatcgcga taactcacga cctgcgtcat
    36901 cgtgatgaaa atgatattga tgccagcggg ctttcggtgt ttgaagaacg cacgtcgcgg
    36961 attatcaaaa acttacccta tatcgccatc gtgggagcat tgattgccgc cgttgccagc
    37021 atgaagattt tcgccggcag tgaagtgtcg atcttcactc tggagaaagc gtactccgca
    37081 ggcgtaacgc cggaacaatc gcaaacgctg atcaaccagg cggcactggc agaatttatg
    37141 cgcgggctgg gttttgtacc gttgattgcc accaccgcct tagcaacggg tgtgtatgca
    37201 gttgcgggct ttacctttgt ttatgcggtg ggctatctct cgccgaatcc gatagttgca
    37261 gcggtattag gcgcagtggt tatttcggcg gaagtcttgc tgcttcgttc gatcggcaaa
    37321 tggctgggac gctacccgtc ggtgcgtaat gcgtcggata acatccgtaa cgccatgaat
    37381 atgctgatgg aagtggcgct gctggtcggt tcgattttcg cagcaattaa gatggcgggt
    37441 tataccggat tctctatcgc ggttgccatt tacttcctca acgaatccct gggccgtccg
    37501 gtacagaaaa tggcggcacc ggtcgtggca gtaatgatca ccggtattct gctgaatgtt
    37561 ctttactggc ttggcctgtt cgttccggct taaggaaggc acctatgaag acgtttcctc
    37621 tgcaaagtct gacgattatt gaggcgcagc aaaagcagtt tgcgctggtg gatagcattt
    37681 gtcgccattt ccccggcagc gagtttctta ctggcggtga tttaggctta acgccaggac
    37741 tgaatcaacc gcgcgttacc cagcgtgtgg aacaggtgct ggctgatgca tttcacgcac
    37801 aggctgcggc gctggtgcag ggtgcgggta ctggcgcgat tcgtgcggcg ttggcggctt
    37861 tgctcaaacc ggggcagcgt cttctggtac atgatgcgcc tgtttacccg acgacacagg
    37921 tcattattga gcagatgggg ctgacgctta ttactgctga tttcaatgac ctgtcggcac
    37981 tgaagcaggt cgtcgacgag caactaccgg atgcggcgct ggtgcagcat acgcgccagc
    38041 agccgcagga cggctacatt ctggcagatg tgctggcaac attgcgctcg gctggcgttc
    38101 ctgcattaac cgatgacaac tatgcggtga tgaaagtggc gcgaattggc tgcgaatgcg
    38161 gggcaaatgt ctcgactttt tcctgcttca agctgtttgg gccggagggt gttggcgcgg
    38221 tggtaggcga tgccgatgtt attagtcgga ttcgctccac gctctattcc ggcggcagcc
    38281 agattcaggg cgctcaggcg ctggaagtct tgtgtgggct ggtgtttgcg ccagtgatgc
    38341 acgcggtgca ggcgggggta tctgaacggt tgctggcttt gcttaacggt ggtgcggtgc
    38401 cggaagtgaa aagcgcggtg attgctaatg cgcagtcgaa ggtgttgatt gtcgagtttc
    38461 atcagccgat tgccgccaga gtgctggaag aggcgcaaaa gcgcggtgcc ttgccttacc
    38521 cggtgggtgc agagtcgaaa tatgaaatcc cgccgctctt ttatcgcctt tccggaacgt
    38581 ttcgccaggc gaatccacaa tcagaacatt gtgcgattcg cattaacccg aatcgcaacg
    38641 gtgaagagac ggtgctgcgg attttgcgtg agagtattgc cagtatttaa tgctggatag
    38701 caccgtcaag ctaaattccg tactgaacgg tcccctcgcc cctttgggga gagggttagg
    38761 gtgaggggaa cgtacgggat gtaaaattca acgcgccagc atcgccggat gcgatgctgg
    38821 cgcatcttat ccggcctacg gttaatgtct tttattatca atcgttaagg tgatgcgatt
    38881 ggcgcggcag aagagttcgg agacgtgcac cggtttgtca ttctgatcaa aggcgatttt
    38941 gctgatgcga aacaacggtt cgcccagttc gcatttcagc cattttgcct gcgggcgggt
    39001 ggcggcaaag atatcgatgg tctttttgtc gctgaccact cgcgtgtcaa aacgctcctg
    39061 aaataactga taggtggagc ttccttcgac gtaaatctca tcaaagtcag gataacgcga
    39121 cagcgggatc catgaactat caataaacag cggctcttta tcgagataca tcacccggca
    39181 gagatggaac acttcgctgt ttccggggat gttcagcttt tcgcaaaacg gcgcggcgct
    39241 gacccgttcc tgttcgatca ctttctcttt cgtcgccttg ccttgtgaga cgccaaaatc
    39301 ggtaaaacca ctgacagtaa gcagggcgtt ttcaactttc tggctttgta caaaggtgcc
    39361 tttgccctgc cagcggatca gtacgccgtc tgccactaag tcgctgatag ctttgcgaat
    39421 agtaatgcgg ctgacgttat attgcgtaca aagctcgttt tcggtaggga tctgttgccc
    39481 ggcctggtaa accccctgcg cgatatcatc cagcagccgc tggcggacgg tagcgtagag
    39541 gagttgatga gagtagcggt ccgtagctga cataatcgac cctgaaataa agaatgaatt
    39601 acacggatta tagggtcgtt ttccggcaag taacagcacg ttggcgcgga gatgctcacg
    39661 ccaacgttat acctaccagg caccgtgata ctgaatggtt ttcgccgcgc acgccgttcc
    39721 ctgcgccatc gcctgcggta atgtcatccc cgcagaccag ccgcaaagga atccggcaat
    39781 gaacgaatct ccggcaccca tggtgtcgat aaccgtcacc ggttcaggag cctgacgcca
    39841 gaactgcgcg ccatcccagg caatgctgcc gttttcaccc agcgtgacaa tcactgttcc
    39901 tgcgccacgg gcaacaatcg ctttcatctt cagacgcagc gcttcgtctt cttgcggtgc
    39961 ggaggcaaag gcaaaatcga gatgcggcac cagtgtctgc cagagcgggc tgtcccactt
    40021 gtcggagaag tcgaaagcgg taagtttgcc cgcagcgtgc agctgtggga atgcgtcttc
    40081 cgcatgtccc cagattgccg cgtgcacaat gtcatactgc gccagccagg cgtaatcctc
    40141 ttcactcagg gcaaagtcgg ccatcacgcc ttcggtgtag tcgccaaaaa cgcgatcatt
    40201 gtcgtgcagt tccacctgag tttgtgcggt aacgccgtgt ttcgtatgga catggctgat
    40261 atcgacgccc atgcgggcga gatcctgctt cagctttgtg ccgtagtcat cgtcacccac
    40321 caaggtaatg catcccggct gtatgccgta gcgagtgcag tacaccgcca cattgaccgc
    40381 attaccgcca gaaaacgctt tattcagttg cgggtagata tcgacgcagt tatcgccgat
    40441 tgtcgccagg gttttcatct ctcatcctcc ggcagcagcg cgcgaaagcg ttccagcgcc
    40501 tggcgggcat agagtctggg ctcgttcata tacatcgtca ccagctccac cgtacagtaa
    40561 ccctcatagc cccgctcaat aatatcgcgc atcagttccc gcagcggcat tttgccttct
    40621 ccaggaatgt aatgcgtgtc gctggccccg tcgctgtcga caatatgcag atgacgataa
    40681 tttatcgccc agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca
    40741 aatgtcgacc atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc
    40801 attagcatta cacacgacgt tcgattcata cggcgttaac ggttcgagga ttagatccat
    40861 gccgatgttt tccgcgtact cacacagttc gcttaggttc tctgccagcc gtccccagat
    40921 aacattaggt ggcgtgagat agcctgcgtg ggccgcggaa atcagcgtat aacccgcgtt
    40981 catctctttt gccatatcca tcgccagctt gatcatgtcg aggctttcgc gacgcatatg
    41041 ttcatcgccc agcatcatgt tatacggata gccgttggtt tctggcgtat agccgataat
    41101 cggcatctga tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc
    41161 cggcgcgaac gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct
    41221 tgcatcacga aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt
    41281 tttcatcggg taactccttg cgtaaatccg gcccgttgcc gggccgggag agggattaat
    41341 attccaccag accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca
    41401 ggtagtagca gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca
    41461 gcccttgcga aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa
    41521 agttaatggc gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg
    41581 gaacgcccgg ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc
    41641 agccgtgcgt ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac
    41701 gcagcggacc cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct
    41761 ggcggccttt ttcttcccag gtgcgtacca gatgaccgag cgcattcggc aacggcttga
    41821 gatcgttttt gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca
    41881 ccacgctgta gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc
    41941 taaattccgc cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg
    42001 cgccgcaggc ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag
    42061 aaacgccaat caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc
    42121 agccggaaat ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt
    42181 gcgccgcgtt gagtggcgag ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca
    42241 ccatctcttc cacgatggcg tgcaccagcg gaacgtcatg gctgagaact ttttccactt
    42301 cctgaaccat attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt
    42361 tattttctcc agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa
    42421 ggcgtaagca ggcagaccag tagcaataac gatgacggcg cagataaggc cgggaatcgg
    42481 tgcccagaca aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat
    42541 cagcccgaaa gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat
    42601 gatggagccg aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag
    42661 gctggtgaga tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga
    42721 gacatccggc gtgttgtatt tcggatgcac atggccgaag catttaaaga acaggttgtc
    42781 tttcgccatc gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag
    42841 aatgacgatc atcgccgtga tggcaacaaa gatcccagcg gtgctgccga gtgcggggat
    42901 ccaggtcagg gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag
    42961 gccggaaatc accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat
    43021 cagcgctcgt ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga
    43081 ggccatgccg gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct
    43141 gcccgttgcg ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt taaaccagaa
    43201 gatcccaagg ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg
    43261 aaacgctgcg ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc
    43321 ggcggcgata aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac
    43381 aatcgccagc gccataatcg acaatgacgg tgcatcgttg gcccagaagc tggcccagcc
    43441 ggagaggaaa gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt
    43501 ttccggataa gcggtggata gttccgcata gacgcacatt tgcgggatca caattaaccc
    43561 gccaatgaca aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc
    43621 cacagataca aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca
    43681 aaatccgagc ttgcgttgga gttcctggct tcccatagcg ataccttttt cttgtccaat
    43741 acgttgtagg ggagagtttg cagtcattta aaaggtaaca tgacaatgca tgatgaatat
    43801 aacaacatat gatgttatgt aatgtggagg aagatcacaa aatttcgcac aggatcgcgc
    43861 tgtggctaat ggatgtagtt atcaaattga atttaaagtg aaaatatttt tacgggcggg
    43921 ggcaagaagg acatagaaac aaatacgccc tcggaaaatc cagagggcgt cgggcaatta
    43981 aaccggtgtt agccgatttc tgtcagagac ttactgtgca gtaggaccgc aaccgccaat
    44041 gattttagaa atagaaattg ccgggtgcag caggtagtcg taagagcagt tcttatcacg
    44101 gttttcaata tgaccagtac atgcagttaa cgtagccagt actgcaccta ccagtgcaac
    44161 tttaataaat ttgttcattt acattcccta tgtat
//
LOCUS       DAZOYS010000013        43271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOYS010000013 DAZOYS010000000
VERSION     DAZOYS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.013"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            52..570
                     /locus_tag="ACHUN9_000602"
     CDS             52..570
                     /locus_tag="ACHUN9_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0694104.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            586..1125
                     /locus_tag="ACHUN9_000603"
     CDS             586..1125
                     /locus_tag="ACHUN9_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0694105.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1220..2797)
                     /gene="guaA"
                     /locus_tag="ACHUN9_000604"
     CDS             complement(1220..2797)
                     /gene="guaA"
                     /locus_tag="ACHUN9_000604"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0694106.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2866..4332)
                     /gene="guaB"
                     /locus_tag="ACHUN9_000605"
     CDS             complement(2866..4332)
                     /gene="guaB"
                     /locus_tag="ACHUN9_000605"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0694107.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4494..5864
                     /gene="xseA"
                     /locus_tag="ACHUN9_000606"
     CDS             4494..5864
                     /gene="xseA"
                     /locus_tag="ACHUN9_000606"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0694108.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5861..6076)
                     /locus_tag="ACHUN9_000607"
     CDS             complement(5861..6076)
                     /locus_tag="ACHUN9_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0694109.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6145..7617)
                     /gene="der"
                     /locus_tag="ACHUN9_000608"
     CDS             complement(6145..7617)
                     /gene="der"
                     /locus_tag="ACHUN9_000608"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0694110.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7735..8913)
                     /gene="bamB"
                     /locus_tag="ACHUN9_000609"
     CDS             complement(7735..8913)
                     /gene="bamB"
                     /locus_tag="ACHUN9_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0694111.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8924..9544)
                     /gene="yfgM"
                     /locus_tag="ACHUN9_000610"
     CDS             complement(8924..9544)
                     /gene="yfgM"
                     /locus_tag="ACHUN9_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0694112.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9562..10836)
                     /gene="hisS"
                     /locus_tag="ACHUN9_000611"
     CDS             complement(9562..10836)
                     /gene="hisS"
                     /locus_tag="ACHUN9_000611"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0694113.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10947..12065)
                     /gene="ispG"
                     /locus_tag="ACHUN9_000612"
                     /gene_synonym="gcpE"
     CDS             complement(10947..12065)
                     /gene="ispG"
                     /locus_tag="ACHUN9_000612"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0694114.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12092..13105)
                     /gene="rodZ"
                     /locus_tag="ACHUN9_000613"
     CDS             complement(12092..13105)
                     /gene="rodZ"
                     /locus_tag="ACHUN9_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0694115.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13390..14544)
                     /locus_tag="ACHUN9_000614"
     CDS             complement(13390..14544)
                     /locus_tag="ACHUN9_000614"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0694116.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14694..15125)
                     /gene="ndk"
                     /locus_tag="ACHUN9_000615"
     CDS             complement(14694..15125)
                     /gene="ndk"
                     /locus_tag="ACHUN9_000615"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0694117.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15274..17586)
                     /gene="pbpC"
                     /locus_tag="ACHUN9_000616"
     CDS             complement(15274..17586)
                     /gene="pbpC"
                     /locus_tag="ACHUN9_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0694118.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17587..22548)
                     /gene="yfhM"
                     /locus_tag="ACHUN9_000617"
     CDS             complement(17587..22548)
                     /gene="yfhM"
                     /locus_tag="ACHUN9_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0694119.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22755..23600
                     /gene="sseA"
                     /locus_tag="ACHUN9_000618"
     CDS             22755..23600
                     /gene="sseA"
                     /locus_tag="ACHUN9_000618"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0694120.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDNLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23936..24061
                     /gene="timP"
                     /locus_tag="ACHUN9_000619"
     CDS             23936..24061
                     /gene="timP"
                     /locus_tag="ACHUN9_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0694121.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24093..24869)
                     /gene="sseB"
                     /locus_tag="ACHUN9_000620"
     CDS             complement(24093..24869)
                     /gene="sseB"
                     /locus_tag="ACHUN9_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0694122.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25011..26294)
                     /gene="pepB"
                     /locus_tag="ACHUN9_000621"
     CDS             complement(25011..26294)
                     /gene="pepB"
                     /locus_tag="ACHUN9_000621"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0694123.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26472..26672)
                     /gene="iscX"
                     /locus_tag="ACHUN9_000622"
     CDS             complement(26472..26672)
                     /gene="iscX"
                     /locus_tag="ACHUN9_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0694124.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26684..27019)
                     /gene="fdx"
                     /locus_tag="ACHUN9_000623"
     CDS             complement(26684..27019)
                     /gene="fdx"
                     /locus_tag="ACHUN9_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0694125.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27021..28871)
                     /gene="hscA"
                     /locus_tag="ACHUN9_000624"
     CDS             complement(27021..28871)
                     /gene="hscA"
                     /locus_tag="ACHUN9_000624"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0694126.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28888..29403)
                     /gene="hscB"
                     /locus_tag="ACHUN9_000625"
     CDS             complement(28888..29403)
                     /gene="hscB"
                     /locus_tag="ACHUN9_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0694127.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29499..29822)
                     /gene="iscA"
                     /locus_tag="ACHUN9_000626"
     CDS             complement(29499..29822)
                     /gene="iscA"
                     /locus_tag="ACHUN9_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0694128.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29839..30225)
                     /gene="iscU"
                     /locus_tag="ACHUN9_000627"
     CDS             complement(29839..30225)
                     /gene="iscU"
                     /locus_tag="ACHUN9_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0694129.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30253..31467)
                     /gene="iscS"
                     /locus_tag="ACHUN9_000628"
     CDS             complement(30253..31467)
                     /gene="iscS"
                     /locus_tag="ACHUN9_000628"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0694130.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31708..32196)
                     /gene="iscR"
                     /locus_tag="ACHUN9_000629"
     CDS             complement(31708..32196)
                     /gene="iscR"
                     /locus_tag="ACHUN9_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0694131.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32497..33234)
                     /gene="trmJ"
                     /locus_tag="ACHUN9_000630"
     CDS             complement(32497..33234)
                     /gene="trmJ"
                     /locus_tag="ACHUN9_000630"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0694132.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33353..34156
                     /gene="suhB"
                     /locus_tag="ACHUN9_000631"
     CDS             33353..34156
                     /gene="suhB"
                     /locus_tag="ACHUN9_000631"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0694133.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34301..35155
                     /locus_tag="ACHUN9_000632"
     CDS             34301..35155
                     /locus_tag="ACHUN9_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0694134.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35346..36626
                     /gene="csiE"
                     /locus_tag="ACHUN9_000633"
     CDS             35346..36626
                     /gene="csiE"
                     /locus_tag="ACHUN9_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0694135.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36618..37757)
                     /locus_tag="ACHUN9_000634"
     CDS             complement(36618..37757)
                     /locus_tag="ACHUN9_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0694136.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37917..38807)
                     /gene="hcaR"
                     /locus_tag="ACHUN9_000635"
     CDS             complement(37917..38807)
                     /gene="hcaR"
                     /locus_tag="ACHUN9_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0694137.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38943..40304
                     /gene="hcaE"
                     /locus_tag="ACHUN9_000636"
     CDS             38943..40304
                     /gene="hcaE"
                     /locus_tag="ACHUN9_000636"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0694138.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40301..40819
                     /gene="hcaF"
                     /locus_tag="ACHUN9_000637"
     CDS             40301..40819
                     /gene="hcaF"
                     /locus_tag="ACHUN9_000637"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0694139.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40819..41139
                     /locus_tag="ACHUN9_000638"
     CDS             40819..41139
                     /locus_tag="ACHUN9_000638"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0694140.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41136..41948
                     /gene="hcaB"
                     /locus_tag="ACHUN9_000639"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41136..41948
                     /gene="hcaB"
                     /locus_tag="ACHUN9_000639"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0694141.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41958..43160
                     /gene="hcaD"
                     /locus_tag="ACHUN9_000640"
     CDS             41958..43160
                     /gene="hcaD"
                     /locus_tag="ACHUN9_000640"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0694142.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10265 a        11950 c        10863 g        10193 t
ORIGIN      
        1 ttttgatgat cttaaatgtc ttatttttcg taatgtgtat aacaaggaat agtgatgaaa
       61 tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat tcactctggc aggatgccag
      121 tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg atcaactgaa taccaaacaa
      181 gaaactaaaa ccgttaatat tatttccatt cttcccgcaa aagttgccgt agacaactcc
      241 caaaataaac ggaacgcaca agccttcggc gcgcttattg gcgcagtcgc tggcggtgtt
      301 atcggccaca acgtcggttc tggcagcaat tccggaacga cggcaggggc agttggcggc
      361 ggagctgtag gcgcggcagc gggttctatg gtgaatgata aaaccttagt ggaaggtgtt
      421 tctttaacat ataaggaagg caccaaagtg tatacctcca cccaggtggg taaagagtgc
      481 cagtttacga caggtttagc cgttgttatt accacaacgt ataacgaaac gcgtattcag
      541 ccaaatacta aatgtcctga aaagagctaa taatcaggag gagtcatgaa gaaagttttt
      601 ctttgcgcca tcttagcctc cttaagctat ccggctatcg cctcatcatt gcaggatcaa
      661 ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg aagagcaaag gcagcatgac
      721 gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga agcaacgtcg cgcaaatgcc
      781 caggccgccg ctaacaaaag agcggcaacg gcagcggcaa ataagaaagc tcgtcaggat
      841 aaactggacg ccgaagccac tgcggacaaa aaacgcgatc aaagttatga agatgagcta
      901 cgcagcttag agattcagaa acaaaaactg acgctggcga aagaagaagc ccgcgttaag
      961 cgagaaaacg aatttatcga tcaggaactg aagcacaaag ctgcgcaaac cgatgtggtg
     1021 caatctgaag ctgacgccaa cagaaatatg actgaaggcg gtcgcgatct gatgaaaagc
     1081 gtgggcaaag cagaagagaa caaatcggac agctggttta attaatcgat gttagtaact
     1141 tcaagcctat gattcttgaa gataaaaaac cctctgtagt aacagagggt tttgttcatt
     1201 catagtgcag ggattaaaat cattcccact caatggtagc tggcggcttg ccgctgatgt
     1261 catacaccac gcgggaaata ccgttcactt cattgataat gcggttggaa acgcgaccga
     1321 ggaaatcgta cggcagacgc gcccagtgtg cggtcataaa gtcgatggtt tcgacagcac
     1381 gcagagagac aacccagtca tacttacgac catcgcccat tacgccaacg gaacgtaccg
     1441 gcaggaacac agtgaacgcc tggctgactt tgtcgtacag gtccgcttta cgcagttctt
     1501 caatgaagat ggcgtcagca cggcgcagca ggtcacagta ctctttcttc acttcaccca
     1561 gcacacgaac gccaaggcct ggtcccggga atgggtgacg gtacaacatg tcgtacggca
     1621 ggcccagctc cagaccaatc ttacgcactt cgtctttgaa cagctctttc agcggttcaa
     1681 ccaggcccat cttcatctct ttcggcaggc cgcccacgtt gtggtgagat ttgatgacgt
     1741 gtgctttacc ggttgcagac gccgcagatt cgataacgtc agggtagatg gtgccctgcg
     1801 ccagccactt cacgtcttcc agtttcagcg cttcttcatc gaatacttca acgaaaacgc
     1861 gaccgatgat tttacgtttt gcttccggat cgttttcgcc agccagcgct gacaggaagc
     1921 gatcttctgc cggaacgtga acaatgttca gaccaaagtg atcgccaaac atatccagaa
     1981 cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac gaatacgcaa gtcaggtttt
     2041 taccgatagc gcggtgcagt agcattgcgg ttacggagga atccacgcca ccagagaggc
     2101 cgaggatgac tttatcgtcg cctacctgct cacggatgcg ggctacagca tcgtcgataa
     2161 ttttcgctgg cgtccacagg gcttcacact ggcagatatc acgcacaaaa cgctccagca
     2221 tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa ctgtacgcca tagaagcgtt
     2281 tttcttcgtt agccataatg gcaaacgggc agctttcggt gctggctacg gtgacgaagt
     2341 cggacgggat agcggtaact ttatcgccgt ggctcatcca gacatcgagc agcggtttac
     2401 cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc actgtcgttt acgacttcaa
     2461 cctgcgcgta gccaaattca cgttcgttag aggcttcaac gtgaccgccc aactgcattg
     2521 ccatggtctg catgccatag caaacgccga ataccggtac gcctgcttca aagacatact
     2581 gcggcgcacg cgggctgttt tcttcagtag tactttccgg gccgccggaa agaataatgc
     2641 cgcttggatt gaagtcacga atttgtgctt ctgtcacatc ccacgcccac agttcgcagt
     2701 aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt gtactgagaa ccgaagtcca
     2761 gaatgaggat gcgatgctta tgaatgtttt ccgtcattga cgcttattcc gaggcaagtg
     2821 aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa gagaatcagg agcccagacg
     2881 gtagttcggg gactctttag taatggtcac gtcgtgaacg tggctttcct gaatgcccgc
     2941 accgctgata cgtacaaact ccgctttagt acgcagttcg tcgatagtac cacagccggt
     3001 cagacccata caggagcgca ggccgcccat ctgctggtga atgatctctt tcaggcgacc
     3061 tttataagct acgcgacctt cgataccttc cggcaccagt ttgtcggcag cgttatcgct
     3121 ctggaaataa cggtcagagg aacctttgga catcgcgccc agggaaccca taccacggta
     3181 agatttgtaa gaacggccct ggtagagttc gatttcaccc ggagattctt cagtacccgc
     3241 cagcatggaa cctaccatca ccgcgcttgc gccagcggcg atagctttgg cgatgtcgcc
     3301 ggagaagcga ataccaccat ctgcgataac cggaataccg gtgccttcca gggcttcaac
     3361 tgcgtcagca acggcagtga tctgcggaac acctacgcca gtaacgatac gagtcgtaca
     3421 gatagaacca gggccgatac cgactttaac cgcgctgcaa ccagcttctg ccaaagcgcg
     3481 tgcacctgca gctgttgcca cgttgccgcc gatgatttgc agatccggat atttagcacg
     3541 ggtttcacgg atacgctgca aaacgccttc tgagtgaccg tgggaggagt cgatcagcag
     3601 aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct tcgttacccg cacctgcgcc
     3661 aactgcagca ccaacacgca gacggccttg ctcgtcttta caggcgttcg gtttacgttc
     3721 cgctttctgg aagtctttca cggtgatcat gccgatcagg tggaattcgt catcaaccac
     3781 cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc accacttcac gggcttcacc
     3841 ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa acgctaaccg gctggttcag
     3901 gtcggtaaca aaacgcacgt cacgaccggt gataataccc accagttcgt tttcttcggt
     3961 aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt tctttcactt cgcgcagcgt
     4021 cgtggttggc agaacagtct gcggatcagt caccacacca gattcgtgtt ttttcacacg
     4081 gcgaacttct tctgcctggc gttcaatgga catgtttttg tggataaagc cgataccgcc
     4141 ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg gtatccattg ctgcggaaag
     4201 catagggata ttcagacgaa tagttttcgt cagctgggtg ctgaggtcag cagtattcgg
     4261 cagaacggta gaatgagcag gaacgaggag aacgtcgtca aacgtcagag cttctttagc
     4321 gatacgtagc atgggcaata tctcgaccag agtggttaat aaatattgcc gcggcattat
     4381 acagagcgta accgattgca tctacccctt tttgcaaaaa atgcttgcta tccccgaagg
     4441 gcgggttact atcgactgaa taacctgctg atttagaatt tgatctcgct cacatgttac
     4501 cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa tcaaacggtt cgtctgctgc
     4561 ttgagcatga gatgggacag gtttggatca gcggcgaaat ctctaatttc acacaaccgg
     4621 cttccggtca ctggtacttt acactcaaag acgacaccgc ccaggtacgc tgcgcgatgt
     4681 tccgcaacag caaccgccgg gtgaccttcc gtccacagca tggacaacaa gttttagttc
     4741 gcgccaatat tacgctctac gagccgcgcg gtgactacca gataatcgtt gagagtatgc
     4801 agccggccgg tgaagggctg ctgcaacaga agtacgaaca gctcaaagcg aagttgcagg
     4861 ctgaaggttt gttcgatcag caatacaaaa aaccacttcc ctcccctgcg cattgcgttg
     4921 gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat tttgcatgtg ttaaaacgtc
     4981 gcgatccttc tctaccggtg atcatctacc ccaccgccgt tcagggcgat gacgcaccgg
     5041 ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa tgagtgcgac gtgttgatcg
     5101 ttgggcgcgg cggcggttcg ctggaagatt tatggagttt taacgacgaa cgcgtagcgc
     5161 gggcgatttt tgccagccgc attccggtcg tcagcgccgt cgggcatgag acggatgtga
     5221 ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc gtctgccgcc gctgaagtag
     5281 tgagccgtaa tcagcaagag ttactgcgcc aggtgcaatc gacccgtcaa cggctggaga
     5341 tggcgatgga ttattatctc gccaaccgca cgcgtcgctt tacgcagatc catcaccgat
     5401 tacagcaaca gcatccgcag ctccggctgg cacgccagca aaccatgctt gagcgcctgc
     5461 aaaagcggat gagctttgcg ctggaaaatc aacttaagcg taccgggcaa cagcagcagc
     5521 ggttaacaca gcggctgaat cagcaaaatc cacagccgaa gattcatcgc gcgcaaacgc
     5581 gcattcagca actggaatat cgtttagcag aaaccctgcg cgtacagctt agcgccacgc
     5641 gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt aagcccactg tcaacgctgg
     5701 cgcgtggata cagcgttact actgctactg acggcaatgt actgaaaaaa gtgaagcaag
     5761 ttaaagcggg tgaaatgcta accacacgtc tggaagacgg ctggatagaa agtgaagtta
     5821 aaaacatcca gccagtaaaa aaatcgcgta aaaaggtgca ttaagccagc acgaattcaa
     5881 cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa gtaatctact gcaccgcagg
     5941 cttttaacac ctgcaacggt tgatggcagt ctggacaaag agctttcatt tcaataattt
     6001 ccccgcagga tgggcaacgc gcatggccgt tatcctgatc aagcacatgc tgacattgtg
     6061 gacaatgcag ttccatatga attcctccgg tagccatccc tctacattca tagagggaat
     6121 ggcagataaa atacttacgg ataattattt atttttcttg atgtgcttca tcagacgctt
     6181 acgtttacgc atctgggttg gcgtcagggt gttacgcttg ttcgcatacg ggttttcccc
     6241 ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc agcgatttgc ggaagtagtt
     6301 catcaagtag cgcttgtagg aatcaggcag gtctttcacc tgattaccgt gaatcaccac
     6361 aatcggcggg ttataaccac cagcgtgggc atatttcagc ttcacacgac gaccgcgtac
     6421 cagcggcggt tggtgatctt caacagccat cgtcatgatg cgcgtcagca tagaggtccc
     6481 cacacgacgg gtggagctgt catacgcttc acgtactgat tcaaacaagt taccaacacc
     6541 actgccgtgc aaggcagaga taaagtgcac acgagcaaaa tcgataaagc ccagacggaa
     6601 gtccagcgtt tctttcacct gctctttcac ttcctggctc aggccatccc acttattcac
     6661 cacaatgaca agtgagcgcc cactattgag aataaagccc agcagcgaga gatcctgatc
     6721 ggaaatacct tcgcgcgcat caatcactaa catcaccacg ttggtatctt caatggcctg
     6781 caacgttttg attacggaga atttctctac agcatcggtg attttgccgc gtttacgtac
     6841 gccagcggtg tcaatgagca catactcacg tccatcgcgt tccattggaa tgtagatact
     6901 gtcacgcgtc gtgccaggca tgtcataaac aacaacgcgc tcttcaccaa gaatacggtt
     6961 agtgagtgta gacttaccta cgttcggacg acccacaatc gccagtttga tcggcagact
     7021 ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc tcttccgctt caaattgcgc
     7081 ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg agatcttcca tccacggcag
     7141 cagcacacgc tccagcagac ttaatacgcc acgaccgtga gatgcggcga tcgggtagat
     7201 ttcacctaaa ccaagcgagt agaaatcaac cactgcctga tcgggatcca gaccgtcagt
     7261 tttgtttgcc accaggaagg tcggtttttc acgagagcgc agatgtttgg caatcgcttc
     7321 atctgccggc atcaagcccg cgcgcgcatc caccataaac agtacgacgt ccgcttcttc
     7381 aatcgccagc agcgactgtt ccgccatgcg ggtttctaca ccgtcttctg tgccatcaat
     7441 cccgccggta tcaatacaga taaactcacg gccttcaatt tccgcacgac cgtacttacg
     7501 gtcacgagtc agacccggga aatccgcaac cagcgcatct cgggtgcgag ttagacggtt
     7561 aaataacgtg gattttccta cgttagggcg cccgacaagc gcgaccacag gtaccatgtt
     7621 taaagcctca tttttataaa tcatcagaca acgcacgcta tattcgcgtc gttgttaaaa
     7681 acaggaaaac ggcccctgtc caggagccgt tttcaaagtg aacgacagag acgattaacg
     7741 tgtaatagag tacacggttc cgtcttttgc ctggatcagc agtttgccgt cagcggcaac
     7801 cggttcagtc tggaaaccgg aactatcaac tttttgctgg gcaacgaaac gaccatcttc
     7861 gacgttaatc cagtgcagat aaccttcact gtcacctacc accaggttgc cattatacag
     7921 caccggcgaa gtcagcaggc gatgcagcag atcgctttgt gtccacagcg taacgccgcc
     7981 atcaatggtc aacgccatca cccggtcatt ttgatcgacc agatagatgc gattgccgtc
     8041 gacgatgaaa tcattcaccg aacccagttc gcgtttccac ataatctgac cactgcgcag
     8101 atcaagcgcc gtcaggttac cattataggc cagagcgaaa acaacgccgt taacaacgac
     8161 gggagtcgtg tcaacatcgc tcagacggtc aatttcggta gaaccggtcg cctgggaaat
     8221 acgctgctgc caaatcatct ggccctgttc catcagcact gcgctgacgc gaccattatc
     8281 gccccccacg acggccgcac caaaagccgt tgccggcgca gactcgccac gcaaagagag
     8341 cgaaggcata tcgaggttaa ctgtccattt gacagcgccg tcagcttcgt tcagcgcttg
     8401 taactgaccg ttactagtgt ggattaacac cagaccgtcg ctgaccaccg ggcgagaaag
     8461 tgcttcaccc gcgactttag tttgccatgc cacagtacca tcgctggtat tcagcgcgta
     8521 aacctgcgcc ttttcgctgc caatgtagac atgcccacca gacacggtca caccgccaga
     8581 aagtaatgca ggctctttag agaaccagcc atctttctcg gccaggctga cagaccagat
     8641 ttctttgcca tcatccgcat tcagcgcttt taccaaacca gcgcggtccg ctgcatagac
     8701 aacgttgtcc gccagtgccg gatgaagatt ggaatagaag ttgccaatgc cgctaccaac
     8761 ggaagtgctc cacgccgtgg tcggcgtaaa ctggttttca acggttggca atggggacat
     8821 ctttaccaca tcttcttcgc tgttaaacag cgaacagccg cttaaaaggg taacggaaag
     8881 cagtcctggc agcagtaatt tacgcaattg catcgggtcc ctctcagatg gacaaattat
     8941 taattttcat ctgcatcatt tcgctcagtg ccggagtaac atcgcttttc acgcctgctt
     9001 cccatgcact acgcgcacct tgcttatcac ctttgctcaa caatgcttta ccacgcaggt
     9061 cggcaacaat ggcagcccac ccttcacctt tgatggtatc aagggttttc agcgcggcat
     9121 cagcctgctt gagctgtacc tgaacgcgag caagacgcag atttatcacg gctttgagat
     9181 tttcatcact cgtgtctgcc agcccctgtt gtaactgggc ggcagctttc tctagttcat
     9241 ttttgtcaac aaattgctgc gcaagttcca aagaagccag cgcaccataa gtatttttat
     9301 tttcagcagc aaatttttcc gccgccggga tgctatccgg tttgccttcg ctcactgcgg
     9361 taaccgcatt ttgataggca agagaagcgg agcgtgcaga atcaacctga tggctgttcc
     9421 agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac cccaacagcc agtgctttgc
     9481 cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc gttttcgttc tcgtaaattt
     9541 ccacgctgtc cttctccttc cttaacccag taacgtgcgc aaatgcgcgg ctacgctatc
     9601 ctgcgcaact gccgtttgct caccagagcg caaatccttc actactgctg tgccgttagc
     9661 cacttcagac tcacccagca ccacagcaac gcgggcaccc catttatcag cacgggcaaa
     9721 ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat ttcacgcccg gtaattcatc
     9781 acgcagacgc tcagctaatg ccatagccgc agattgtgta tcagcacctg aagccaccag
     9841 gtatatatcg acaacaggat cggctttaaa ttccggatta acggcctgta ctaacaatac
     9901 aagacgttcg aggcccatcg caaaaccgac tgccggtgtt gcacgaccgc ccagttgttc
     9961 caccagaccg tcataacgac cgcctgcaca cacggtgccc tgggagccga ggctgttagt
    10021 cacccactcg aaaacggtac ggttatagta atccagacca cgcaccagac gctggtttac
    10081 ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga ccggcaaaat gctcacgaga
    10141 ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg agaagcgcct gcacttccgg
    10201 attttttgaa tccagcacgc gcagcgggtt agtgtacatg cggcgtttgc agtcttcgtc
    10261 cagcttttct ttatgctgct caaggaatgc caccagcgca tcgcggtaat tggcgcgtgc
    10321 ttccagcgaa ccaatagagt tcagctcaag agttacgtgt tcgaagatac ccagcgcacg
    10381 ccaccagcgg gcagtgagca taatcagttc agcgtcgata tccggacctt gcagaccgaa
    10441 aacttcgcag cccaactgat ggaactgacg ataacgccct ttctgcggac gctcgtgacg
    10501 gaacatcggc ccgatatacc acagacgctg ttcctgattg tacagaagac catgctcgat
    10561 gccggcgcgt acacagcccg ccgtcccttc agggcgcaga gtcaggctgt cgccattgcg
    10621 atcctcaaag gtgtacatct ctttttcaac cacgtcggtg acttcaccaa tcgcacgttt
    10681 gaatagcggg gtctgctcta caatcggcaa gcggatttca ctgtaaccgt agctgccgag
    10741 cacgtttttc agtgtgcctt caatgcgctg ccagatggcc gtttcgccag gcaggtaatc
    10801 gttcatgccg cgaatggctt gaatgttttt tgccacgttt attctctttc tgaatataaa
    10861 aatgaaccct caacgcttcc ctcaatgttt cgggagccat gcgggttcaa tcatacacgg
    10921 gaagcgaggc gcttcccatc acgttattat ttttcaacct gctgaacgtc aattcgacgc
    10981 gcttcgtcca gctgactggc tttcgcacga atgcgtgctt ccagctggtc gatcatatcg
    11041 ttgttgtcca gacggtcttt gcgcacgcca tcttcataga ggccgctttt cttgttgccg
    11101 ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc cattcaccac gcagccgata
    11161 atcgaaacgt ccatcggagt gatgatatct tccaggcgtt gctccagcgc gttaaccgta
    11221 ccgataacat caaattcctg acgcgaacag gtcgggcagg cgatgaagtt aatccctcgc
    11281 gaacggatac gcagcgattt caaaatatcg aaaccgactt tgatctcttc gaccggatcg
    11341 gccgccagcg atacgcgcag cgtgtcgccg atgccttccg acagcagcag acctaaacca
    11401 atggcggatt ttactgcccc gctgcgtgca ccaccggctt cggtgatccc cagatgcagc
    11461 ggctgatcga tctgttttgc cagcaaacga taagactcaa cagcgaggaa gacgtcagac
    11521 gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat cgagatgatc aacatgacgc
    11581 atggcggatt ccagcaacgc ctgcggcgtc ggttcgccat acttttcttg cagatctttt
    11641 tccagcgatc cggcgttaac gccaatacgg atcggaatgt ttttatcgcg cgcacagtca
    11701 accaccatgc gaatacgctc ttcattaccg atattgccag ggttaatacg cagacaatcg
    11761 acgccgtatt ccgctacttt cagcgcaatg cgatagtcga agtggatgtc agccaccagc
    11821 ggcacgttaa cccgctgttt gatgagtttg aacgcttctg ccgcgtccat cgtcggtacg
    11881 gagacacgga cgatatcagc gccaacgcgt tccagcgcct tgatttgatt gaccgttgct
    11941 tcgacatctg tcgtacgcgt attggtcatg gactgtacgg cgatgggagc accatcgcca
    12001 atcggcacat tcccaacgta aatacgtgtt gattttctac gttgaattgg agcctggtta
    12061 tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc cggtgattgt tcggcattga
    12121 gggtcagacg cgcaacctgg ttagttctga taaaacgact cagatcgaca ggtttccctt
    12181 gatactggat ctgtactgcg gctggcgcac caattttcag tttatacggt gcctggccgg
    12241 ttaaattcaa attaccgtct ttacgctgca tcccgctaaa caatttttta ccggtagcat
    12301 cagtgacctc cagccagcaa tcggcagtaa agttcatcac cagcgcattc ggatcagccg
    12361 ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc agcaccatct ggcgttgttg
    12421 ctgccgttgg taccggggtc gcggcggtat caacatttgc ctgcgaaggc gaaacaaccg
    12481 cattctgttg cggatcaaca gctggtgctg gcgcagttac ggcaggtgtt tgcgtgttgg
    12541 ttgcggtagt atccacagac gctggcggcg tggacgttgt agccgggtct gtagttgtcg
    12601 acgtatttaa cgggacactc tgcccctgct cgctattact gctcagttct gccgaagatt
    12661 gatcggccat agtggtgatc tcttcctgct gagctttgtg gtcttgccac caccaggcac
    12721 cgctcaggcc gataaccaca aacaacacca gccaggtgaa ggtcatcagc cagccatcac
    12781 gttttttgcg gcgtttaccg agggaaaaac tctgcattgg cgcaactttt gcagcccgta
    12841 gtggagcctg cttttccagc cctggcagca gttcttcttc tggaatatgt accagacgcg
    12901 cataagagcg gatatatccg cgcaggaatg ttgaagcaag atcggcgggt gccttatctt
    12961 cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg ctcggcaacg gcctgctgac
    13021 taagtcctag ttgttcgcga gcattacgca ggcgagcgcc ggtagtaagt gcttcatttt
    13081 ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt ttaaatgaga attgagatgc
    13141 cggtgaacca tcatgctcac ccacaccgcg aaacatccgt taagttaacc gttatcatac
    13201 agtataagac cgtctgtgtg ctcgtgacaa agcccacatt cactaaggct aacttactgt
    13261 tgcatcgtta catactgcct taaagtcagc aaaaacgcac cgttaatatt gaccagacaa
    13321 ttgcaactta atacttcatt cataaaacat tacgccacgg tacataaagt aaccgtggcg
    13381 taatggctat cagaccgctt taatgtcgat ggcttcaccc tgcatccgtt tacgcagggt
    13441 acgtttcgta cggtcgataa catcgcccgc caactgacca caggcagcat caatatcatc
    13501 accacgagtt ttacgcacaa tagtggtgaa accgtagctc atcagcactt ttgagaaacg
    13561 gtcgatacgg ctgttcgagc tgcgtccata cggcgcaccc gggaacgggt tccacgggat
    13621 caggttgatc ttacacggcg tatctttcag cagttccgcc agttggtgcg cgtgttcagt
    13681 gccgtcgtta acgtggtcaa gcatcacgta ttcaatagtg actcggccct gattggcgtt
    13741 ggatttctcc agataacggc gcaccgcagc aaggaacgtt tcgatattgt actttttgtt
    13801 gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg tgcagggaaa ttgccagtgc
    13861 aacgtcgatc atatcgccca gtttatccag cgccggaact acaccggaag tagaaagcgt
    13921 gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc atgatttcca tcgccggaac
    13981 gacgttgttc aggttgagca gcggctcgcc catgcccatc atcactacgt tagtgattgg
    14041 ccgctgaccg gtgacttttg ctgcgccgac gattttcgcc gcccgccaca cctggccgat
    14101 aatttccgac acccgcaggt tgcggttaaa gccctgctgg gcggtggaac agaatttaca
    14161 ctccagcgca caccccacct gcgaagagac gcagagcgtg gcacggtcgt cttccgggat
    14221 atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc catttaatgg tgccgtcgga
    14281 tgaacgctgt tcttcaacca cttccggtgc gcggatctcc gccacctctt tcagtttgcc
    14341 gcgcaacact ttgttgatgt cggtcatctc atcaaagttg tcgcagcaat agtgatacat
    14401 ccacttcatc acctgatcgg cgcggaaggg tttttcacct aaatctttaa aaaactcccg
    14461 catctgctga cggttgagat ccagcaggtt gatttttcca tctttcgtgg tgacgttttc
    14521 aggtgtgact aattgttcag acatatgcta ttccggcctc gttattacac gttatggccc
    14581 ctggagggtt gaaaaaagaa acgccccggt gagcggctgc tcgtccgggg gcgctgcatt
    14641 gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc atttacgaaa ttattaacgg
    14701 gtgcgcgggc acacttcgcc ttcgccgaag aaataagcga tttcgcgagc ggcagattcg
    14761 acggaatcag aaccgtgggt accgttttcg gtcaggctgt cagcgtaatc agcgcgcaga
    14821 gtaccggcca gtgcgtttgc cggattggtc gcgcccagca gatcgcggtg acgctgaacg
    14881 gcgttttcac cttccagcac ggaaaccacg atcgggccag aggtcatgaa ttcaaccaga
    14941 ccatcaaaga acggttttcc atcgtgttca gcataaaagc cacgagcctg ttcaacggtc
    15001 aggtgcagca ttttggtgcc gacaattttg aaccctgcag cttcaaagcg cgcaaagata
    15061 ttaccaatga cgttttttgc taccgcgttc ggtttgatga tggaaaaagt acgttcaata
    15121 gccattttta cctctgtaaa ttgttctgtt gttgtctgta ccagcgtacg agatggcgcg
    15181 gattataatg agcaacaggg ccgttgacta ttgatgaagg taacattttt ttaaaataaa
    15241 atgagtttta gcaacaatca gggacaaaac agactattgc atgacaaatt tcactgtcgc
    15301 gatttgcccc acgtcatcca tcaccagcaa ttgataatca cctttatccg tcaaatgcag
    15361 ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc agaaaccacc agcgttcacc
    15421 tgcccctcca cttgattgca acggcaaagt cgcttccgct gcgcccggta aacgtttaat
    15481 aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc tgggcatcgt gaccgtatgg
    15541 cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga cgttctgatg cgggcagcca
    15601 gggttccagc ggtagcggcc agacatttac catttcttgc ttcgcctgcg ggcagtcagc
    15661 ggcgacgcgc tgccctttgt catccagcca gatggggaag cgaatgccat taatcccttc
    15721 ctgctctgac agtaacagcg ttggcggctg gctgccatcc agcagccagg ttgccaggcg
    15781 gcggcgacag ttaccgtcac cttccggcaa agactgtccg cccggccagc agataacgcc
    15841 acgagtgact gagttcgggc gcgggtcttc cggcagattc gcactgcgcg acagtaagat
    15901 attattgacc tgattcaaca atggtacggc actggcaaag ccaaactgac caacaacggg
    15961 cgtgccgtca ggtctgccag tccagatccc aatgacatag cgagcgttaa ccccaatcgc
    16021 ccaggcgtca cgatagccat agctggtgcc cgttttccat gccagtgggg cgacgcgcgg
    16081 caaggcacta tccggcaagg gttgcgcttc atcagccata atccggcgaa tgatccacgc
    16141 cgcccccgac gacattaaag gccgttcaag cagcggatcg tcaggctgta agcgcaattt
    16201 gcctgccttg ccgtggcgag caaacgcggt atacgctgcc gccatatctt ccagttttgc
    16261 accagcgccg ccgagaatga gtgaaagatt cggcgcagcg ccgttgggca aatataacgg
    16321 caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt ccataggctt ccagcacctg
    16381 cacagcaggt aagttcagcg agcgcaccag cgcctcgctc atgctgatcg ggccatgaaa
    16441 accgctatca aagttacctg gtcgataatc accggtgcgc cgggggacgt cttgcagcag
    16501 tgatgccggg tggatcaagc cttcatccag cgccagacca taaacaaacg gtttgagcac
    16561 cgatcctggc gatcggatcg cattgaccat atcaacatga ccaaagcgtg aatcatcgtt
    16621 gagatcaacc gatcccaccc agccgcgaac acgcatatcg gtatgatcaa ccacgatcat
    16681 cgccagtgag ctgcgcggtg gcaaccgccc tttccagttt tgcgccagtt cttccagacg
    16741 tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg cttttgcttt taccgagcat
    16801 catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg ggtgccagcc agatgggttc
    16861 ttcccttgac tcttttacct gctcacggga ccacacacct tgcgcagcca tccgttcgag
    16921 cactttatta cgcgcggctt cggcacgctc cggccaacga tccgggcgaa gacggctggg
    16981 cgcttgcggc aaaaccgcca gcattgccgc ctcggaatag cttaaattcg caggcgactt
    17041 tccgagataa gcccaacttg ccgcaccgat cccctgcaac gtaccgccaa acggagcgcg
    17101 gttaagatac aaggtcagaa tttcacgctt agacagatgc cattccagtt gcaacgcgcg
    17161 ccagagctgg cgaattttgc cgccaaatgt tttggggtga ggatcaagca gacgagcaac
    17221 ctgcatagtg agcgtgctgc caccggaaat aacccgtccc gaagtgagat cttgccatgc
    17281 tgcgcgcgcc accgagaatg gattcacccc cggatgcttc cagaaccagc gatcttcata
    17341 attgatcagc gcttcaaggt aacgtggaga aacatcttcg attgttaccg gataacgcca
    17401 gatgccgtca gcatcggcga agcgccagag cggcgtacca tcctgcgcca cgaccactcg
    17461 tgcgggattg acttcatgca gcggtagagg ccagagttta tctgctcccc acgctgcaag
    17521 aaaaaggaga aatggcgcag ctgccagcgt tatccagcag ccgcgtttgg ttaacaagcg
    17581 aggcatttac ggtcggacaa tcagcagatc ttcagccgcg ccggtcgccc gccattgggg
    17641 aacatacatt gattccacca tcggttgcgg tacctgatac gttcccggcg tcaccgcccg
    17701 cgccagatac accagcgtta ccggttggta ttcatcaacg gcaaccgccg ccacaaagcg
    17761 atcgtcacgg aactcaatgt gcttaatact cgcctgctgc atctggttca gtaagttctg
    17821 cacttcgcca ccactttgct ccaggctggc gctaccgttc gccagattct ggttttccag
    17881 ttccagaccc acaggcagca gatccacgac taacgcatcc ggcacgctgt tactggcttt
    17941 tacctgcaac cacaccagca ccagatcgcc gctacgtaac gagtccagcg atttgctctt
    18001 accatcagta ccaagaatat gacgctcgat ttgcagcaca ttgttcgccg gtaaaggtgc
    18061 ggattgcgga taaccgctgg catccatacg caaccataac ggctgatcgc cactgttgct
    18121 cacctgcaag gtgacaagtt gatcgctatt cagattgctg ttttgcgctt tctcgcctgt
    18181 cagcggctca gctgagaaag aggtttgcgc ctgccattta cccggtaaat cctgaatcgt
    18241 acgggcagcc aggaacaacg cgttactttc ctgcgtcgat agccagcgtt caccaaacgc
    18301 ctgctgcgaa agtgtgttca gcaaagtgta ctgctcatcg ggtagcagtt tattttcttc
    18361 cagcaaggag agcattaacg cgttgtcgcg cagtggacta ccgtaatcac ccagccatat
    18421 ccgctcatca ctatttctcg gcgttttcag cgccagcgca atcgcttctt caccacgcgt
    18481 cgcatcaccc atggttttca gcgcaatgcc aagttgcagc agcggtaaac cagaagcggc
    18541 atctgcacga tgctcccaga tttcacgcag cgcacccagt ggtgcttttt gctgacgggc
    18601 cagcaccagc gcagcgtaag actgaacggc gaatttactg gctttgagat tatccgcgta
    18661 cgggatcgac atcatgcccg gatcttgtaa atagcgcagc agacgctcat taccccggtt
    18721 aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg cggaccagga aatccatcac
    18781 gtaagccgtc agccagtact cttcgtcacc gtttttatcc cacagcgcaa agccgccgtt
    18841 atcacgttgc atttgcagca aacgggaaat gccgatatcg accgatgcac ggcgtttctc
    18901 atcactgtcg cctttgatgc ccaacgcctg cagttgggct gcgttggtat aaagtgacgg
    18961 gaacaggccg ctggcggttt gctcaagaca gccgtacgga tacgctttta actctttgat
    19021 ataacgtgca atgttcagcg gtggtttgcc gctcaacaac aattgccctt ccagcgtaac
    19081 aggcgagaag ttttgcaatc catccgccgg aatcgcccat gtctcaccag gctgtaacgc
    19141 cgtaccgtaa ttaaccgttt gtgccgggaa cgccggacgg acgccgattt tccactgctt
    19201 atgctgatcg gcaacggttt cacccggtaa cgctaacccg ctaatggtgg cctgaatttc
    19261 tccatcgcca taacccggca atgctcgcac cgggataaac agcgtagtac gcacacctgg
    19321 cgctaattca acggctgcgg gtgaatcgct gaccagttca agcaaaccac tggcggtcag
    19381 ggcaacgttc agtttttgcg gtttatcggt aagattagtg atatccagcg tcagacgcga
    19441 ggtatcgcca ctcgccataa agcgcggcat gttcagttca gcaatcaccg gtgcggcaac
    19501 tatcactttg ctttcgttgc taccgaaatc atctgccgtc caggcttgcg ccatgacgcg
    19561 caattcaccg ttaaaatcgc caatcggcag tgtaaccgag ccttcgccct gttcgttgag
    19621 cgttaccgac agcgcctgct gcgcgacaat attgacgtga ttgaccggcg gtttaccacc
    19681 acgtttcagc tcatcaccat cgccaccgaa acgcagagct gccagacgcc cctgaccttc
    19741 aataacctga ccgtaaatat cgtaaatgtc tgcgccatag cgtttctgac caaagaacgc
    19801 ctgccacgga tctggcgtaa cgtagtcagt aatattcagc acaccactat caacggcaga
    19861 caccagcaca ttcacctgtt taggcttctc gccattttta gtgctggctt taattttcac
    19921 ggttaatggt tgattgggac gcatttttgc tggtgtttcc agcgccagat cgaggcgacg
    19981 gttttcatca ccaagcggca gatgcaacac accaaccgcg cgttttggcg tcgcggagcg
    20041 agatttatcg ccaggacgca ccaccagcgt actcaaataa agatcatgac gattccaggt
    20101 tttatcgacc ggaatcgtca gatccagccc ttgagccgga acatcaatct cttgccacca
    20161 cagcggccct tcactggact cgaccatcgc ataaccttta cccgccgttg gcgcggcaat
    20221 atgcaactta atggtatcgc cggggcgata actggcttta tctagtttca gcgtgacacg
    20281 gtcgggtcgc actgcgccgc tcccgtcgct gttgtcctgc cagctatagc cagcccaaaa
    20341 acgcacgcta ctgaccgctt cattcggcgc tttgacttcc agacgataag cgccccactc
    20401 taccgggaaa ctgaccttac cggtttcatc cgcctgaaga tccagagttt gttcattttc
    20461 gatcagatct ttttgatcaa actgtgactg ccagccttca tcttctgacc agttccagta
    20521 gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc gacacggctt ttttcacgcc
    20581 ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg ccttcatcaa caatgggctg
    20641 tttcaccgtg ctgtcagtgc gataatcgta aaccgatttc aaggcgaact gcggacggag
    20701 ccccggcaat gcatcggcag gccagatagc ctgctcagcg cggcgcgtca ccgggcgacc
    20761 gcccgattcc agcaaactgc cctggaaaat aacctgcaat ggggaatgcg tttccttcca
    20821 ctggctttct gtagaaactt cgccgcgccc tttatcatcc agcgtcaact gaacttcatc
    20881 cagcgtgcgg gaaaggtttt cggcagctat atcgccgaat tcaaaaccag gtaaggctga
    20941 cacagcctca cgcagtgggc gcaggaaaag ttgcccttgc aaagtattac cattagcagg
    21001 cgcaccgtac aggtagtatc ccaccacgga gaatttcact tcatcattcg gcgtaagcgg
    21061 ggttttctca ccggtcagat tcagcgccat acgctctggc ataaaatctt cgacgtggaa
    21121 atcccacatc cggtactggt tatcgcccgt gttggcgcga atatgccaca taccggttgc
    21181 cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca ttctccggct gactaacgac
    21241 gttcctgagt acctgcccgt caggtttaat cacgtctaac ttgatgggtt gatcgggcaa
    21301 tgctttaccg tctgcatcgc gcagtaaacc attgaggatt accgtttcac ctgggcgata
    21361 aagatcgcgt gggccaaaca tgaaaaactg tttgctatag cctggcgcgc cagcaatgtt
    21421 aaattctgct aagtccagcg ccggaagttt taaatcgagt agcgttgtct gaccgtcttt
    21481 acgtgccagc agtaatgccg catttttatc attttccagc tgcacatgcc cctgagcgtc
    21541 actggttgcc tgagtcagag tctgtccttt ctcatttaat aaagagactt caattccttg
    21601 ctgggccgcg ccgttttcca gactttgggt aaagatatcc agacgattgt gataacggtg
    21661 agctgaaacg ccgatatcac ttaacgtaaa cagcgtcgcg ggattactgt aatcgtaacg
    21721 tccagcctga ttcatcacag ccagatacac gcccgcctgc tgaagcggtt tgatatcgcc
    21781 cagcggcagc aataattttt cacgggtgtt acgcgcagga ttgagatcaa accgtccggt
    21841 gtagaccaga tccgccatct gcagcagttt gtctgactgc cagttcgcca gcgaattgcg
    21901 gtattcccat tggctaatga atgctggcag agattctggc ttaacgcgga agaagttaac
    21961 atcgacatta ttaacgttga gcgccattac cggcagccct tcaacgactt tgccaggcag
    22021 cagcgaacca cggctggcaa aaccgacgct gggttggatg tcgcgggtag ttatagtttt
    22081 ttcgtaatct ttactgaagg ttgcgttgtt gagtgctttg acctccttgc caatagtaac
    22141 gatcaaatca cgtttcggtt cgaggtggcg taaacgtagc tctttaagat tatctgacag
    22201 ctcccaggca ccatccactt tgccgctttt tttatcgacg acatgaataa cgcgtgagaa
    22261 atcctgatcc gggtcgagag ggatggagaa cgtcagcacc agcgtggctg caccatcaag
    22321 ttggacttca gagagatcca gcaatgtcag caccttaccg gcactctgtt gggccagttt
    22381 ttgtctctcc ggcacggaga gttttgctga actcgcgttt tctgaagagg ccgctttagt
    22441 aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg ttatcgttgt tgtcgcaccc
    22501 tgccagcgcc agcattagca tgcaggcggc tacgcgtaac tttttcattt ttcatccctg
    22561 ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg tgagaattta cgttatgtaa
    22621 ttcagtatca cgcccataca atccgaattt tagtgagaat tgtggctcag acagccaaat
    22681 cagcctcttt cgcttgtcgc ttttcactaa acatccgaca atttagccta accccggcaa
    22741 aaatggagat gcctatgtcc acgacatggt ttgtaggagc cgactggctc gccgaacata
    22801 ttgatgaccc ggaaattcag attatcgatg cccgcatggc gtcgcctgga caggaagatc
    22861 gtaacgttgc tcaggagtat ctgaatggac atattcccgg cgcagtgttt tttgatatcg
    22921 aagcgctttc tgatcacact tccccgcttc cgcacatgct gccgcgcccg gaaacgttcg
    22981 ccgtggcgat gcgtgaatta ggcgttaacc aggataagca cctgattgtc tatgacgaag
    23041 gtaatctttt ctcagcccca cgagcatggt ggatgctgcg cacctttggt gtagagaaag
    23101 tgtcgattct ggggggtgga cttgcaggct ggcagcgcga taatctgctg ttagaagaag
    23161 gtgcagtaga gctgccggaa ggagagttta acgccgcgtt taatcctgaa gccgtggtga
    23221 aagtaaccga tgtattattg gcaagccatg aaaatacggc gcaaattatt gatgcccgcc
    23281 cggctgcacg ttttaacgca gaagttgatg aacctcgccc aggtttacgt cgcggacata
    23341 ttcccggagc actgaatgtt ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga
    23401 ccgatgaact ggatgcgata ttttttggtc gcggcgtcag ctacgacaaa ccaattatcg
    23461 tcagctgcgg ctctggtgta acggcagccg tggttttgtt agcactcgcg acgctggatg
    23521 tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac
    23581 cggttgagcc agtgaaataa gtattttaca ggcaataaaa aaccgccgaa tttggcggtt
    23641 ttttattgct ggtctggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
    23701 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
    23761 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
    23821 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
    23881 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
    23941 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
    24001 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
    24061 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
    24121 cgcaaaaaac caccccagcg acgttcatag aatggcacaa tatgttcggt aataaagtgg
    24181 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
    24241 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
    24301 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
    24361 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
    24421 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
    24481 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
    24541 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
    24601 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
    24661 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
    24721 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
    24781 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
    24841 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
    24901 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
    24961 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
    25021 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
    25081 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
    25141 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
    25201 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
    25261 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gcaaacgacc ggcaagcgca
    25321 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
    25381 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
    25441 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
    25501 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
    25561 aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg caaatgccag cgccccggta
    25621 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
    25681 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta cactggcgct
    25741 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
    25801 cgtccgacgg tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
    25861 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
    25921 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
    25981 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
    26041 cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc agcagcgatc cgcatcccag
    26101 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg cgccgcacgc
    26161 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
    26221 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
    26281 atcgcttctg tcattttagt tatccttctt tgtaaaaggg ccgccagttg gcagccccag
    26341 gatttttccg ctaaatgatt cgcgttgcag aaaggcggca agtgagtgaa gccccaggag
    26401 catagataac tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca
    26461 tgacgcggaa attattcggc ctcgtccagc cagactaaca aaatcgcttc gaggattttc
    26521 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
    26581 tcggtgaatc gaaccgtttt cggatcaaga tcaggatacg catcgtacag tgcttcgcca
    26641 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
    26701 ggttgatagt gtaacgcggg atttcgacta ctaaatcttc gtcggtaacg cgtgcctggc
    26761 agcttaaacg gctttcgggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
    26821 ctgagctttc tggcagcgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
    26881 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagagct gcgtcgagaa
    26941 tggtttcacc gctattagct tccagaacag cgccatcagg acagagatcc tgatgaggca
    27001 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
    27061 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
    27121 gcttgttcga tggcgtcaac gtcatcgccc tgcgccactt cactcaggtg agcggcagca
    27181 tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg
    27241 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat tcgggctttt
    27301 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
    27361 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
    27421 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccacca
    27481 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
    27541 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
    27601 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
    27661 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
    27721 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
    27781 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
    27841 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
    27901 acacccgcgt ctttaagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
    27961 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
    28021 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
    28081 tgaacgcggt tatcgctacg atcaggaatg cccgcctgct cgcgaatgta atccgccagc
    28141 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
    28201 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
    28261 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
    28321 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
    28381 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
    28441 gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg caccgggttc
    28501 agcagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
    28561 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
    28621 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
    28681 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
    28741 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
    28801 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
    28861 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
    28921 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
    28981 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
    29041 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
    29101 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
    29161 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
    29221 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
    29281 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
    29341 gctcagcgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaagg tgaagtaatc
    29401 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
    29461 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
    29521 caaccacact catctttgac gttcgggttg gtgaatttaa acccttcgtt caggccttct
    29581 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
    29641 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
    29701 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
    29761 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
    29821 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
    29881 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
    29941 ttcagcaata tcggtatttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
    30001 ttcggtgacc agggagctgg aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc
    30061 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcatcacgt cgccacaggc
    30121 cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
    30181 acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
    30241 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
    30301 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
    30361 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
    30421 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
    30481 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
    30541 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
    30601 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
    30661 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcctcgc ccattccgac
    30721 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
    30781 ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac agcgcaccga tacctttcgg
    30841 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
    30901 aggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
    30961 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
    31021 ggaaacaagg atggtgtcat cacgcatcgc tgcttcaagt tctttcagat cgataatgcc
    31081 gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg cgctccagct gacggcaggt
    31141 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
    31201 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
    31261 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
    31321 ttcttcagcc tgccagccga aacggtgaga acgggaggcc gggttaccaa aggttccgtc
    31381 catcgtcata aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
    31441 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
    31501 ggaaccaggc taccggctgg atgtacgacc gtgtttacgg agcgcgatac gccccggcct
    31561 gattctaata tcttgttatc tttaacctaa ctgtttcggg tttcagcaag ttgacaaact
    31621 tacgaatctc cgggagctta cataagtaag tgaccggagt gagtatgtgc agtcaacgca
    31681 gatgcaacac gaaacaggac ggttaaatta agcgcgtaac ttaacgtcga tcgcgtcttg
    31741 tatgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca gacacatcca gcacttcctg
    31801 gttattaacc agttcgccta aagtaatgtt gttgagaaaa ccggtgagac ggtcgctcaa
    31861 atcacgccac agcgcgtggg tcaggcattt atcgccgccc tggcaaccgc ctttaccctg
    31921 acaacgggta gcatctacag attcgtcaac ggcgctaatc acttcgccaa cggcgatgct
    31981 gctggcatct ttgcctaaca gataaccacc gcctggtcca cgtacgctgg aaaccagacc
    32041 atttttacgc agacgggaaa acagttgttc cagataagaa agggaaattc cctgacgttc
    32101 ggaaatatca gccaacggta ccgggcccgc ttcagagttg agcgcaacgt caagcattgc
    32161 ggtcacggca tagcgccctt tagatgtcag tctcatgtct tacttcacct caaactcgcc
    32221 cctgcccggg gttttttatt gtaaaagtgg gggtattgca tagcagggtc aagtctgaca
    32281 ttcccgagta aattggtcaa ctatttactt gactgattta gtcgggtatt taaccttcag
    32341 tgccattttt ttatcgtggc gtttgtacct gttgtccttc cagaccatgg gcgggttgag
    32401 ggcaattatt cgcattagcg ctggcctctt cccctcaccc taaccctctc cccgaagggg
    32461 cgaggggacc gtccgtgcat ttatctggct tagccctcag gccttattac ctttattctg
    32521 ctgctctata gaagccagaa tcccgcgcag gatattcaat tcctggcttt ccgggcgcgc
    32581 acgggtaaac agacggcgca atttattcat cacctgcccc ggatggtttt cacggataaa
    32641 accggttgcc agcagcgttt gttccagatg accataaaaa cgctccagat catcgaccag
    32701 cggatacggc gtctcttcat gctcgacctg ctcgccattt tcctgagttg ccagccaggc
    32761 catacgcact tcataggcaa taacctgaac cgccattgcc aagttcagcg aactgtattc
    32821 cgggtttgcc gcaatcgcga catgataatg gcatttctgc aactcttcat tggtcaagcc
    32881 gacgcgctca cgaccaaaca ccagcgccac cggggtattt gccgcttcag cgacgctttt
    32941 cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc gtgcgggagc gtgcgctggt
    33001 gcccaccacc aggctacaac cggctaacgc ttcgtcgagc gtatcgacga tgtgagcatt
    33061 accaatcaca tcgctggccc ctgctgccag ggcaatcgcc tgggagtcgg gtttcaccag
    33121 tggattaacc agccacagat tggttaatcc cattgttttc atggcacggg caacagaacc
    33181 catattgccg gtgtgtgacg tctccaccag cacaattcga atattttgca gcattgtctt
    33241 tcttcatcta aagattattc acgcatctta tcataaaacg aagacagatg ccgatctcgc
    33301 tgctatactc tgcgccgttt tcccgttctt taacatccag tgagagagac cgatgcatcc
    33361 gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg ccaaaaacta
    33421 tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt aacgatttcg tgaccaacgt
    33481 agataaagct gccgaagcgg tgattatcga cacgattcgt aaatcttact cacagcacac
    33541 catcatcacc gaagaaagcg gtgaacttga aggtactgat caggatgttc aatgggttat
    33601 cgatccactg gatggcacta ccaactttat caaacgtctg ccgcacttcg cggtatctat
    33661 cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta tacgatccta tgcgtaacga
    33721 actgttcacc gccactcgcg gtcagggcgc acagctgaac ggctaccgtc tgcgcggtag
    33781 caccgctcgc gatctcgacg gtaccattct ggcgaccggc ttcccgttca aagcaaaaca
    33841 gtacgccact acctacatca acatcgtcgg caaactgttc aacgaatgtg cagacttccg
    33901 tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg ttgacggttt
    33961 ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg ttcgtgaagc
    34021 aggcggcatc gtcagcgact tcaccggtgg tcataactac atgctgaccg gtaacatcgt
    34081 tgctggtaac ccgcgcgttg tgaaagccat gctggcgaac atgcgtgacg agttaagcga
    34141 cgctctgaag cgttaatgac tcaggcgggt gatgtcactc acccgccctc gcctttcagg
    34201 cgctattctg aaatacttcc tcaccgcttt actttctttc gtcactctcc caccatcttt
    34261 ccccgattaa tggatgataa agaagtcaca aggaaaggtt atggcgctgc cagtgaacaa
    34321 acgcgttctc aaaattctgt ttattctctt tgttgttgcc ttctgcgttt atttagtgcc
    34381 gcgcgttgcc atcaacttct tctattatcc tgacgataaa atttacggtc ccgacccctg
    34441 gtcggcggaa tccgtcgaat ttacggctaa ggacggtact cgtctgcaag gctggtttat
    34501 tccttcttcg acgggccctg ctgacaatgc catcgcaacc atcattcatg ctcacggcaa
    34561 tgccggaaat atgtccgccc actggccgct ggtcagttgg ttacccgagc gtaatttcaa
    34621 cgtttttatg tttgattatc gcgggtttgg taaatcaaaa ggcacgccgt cccaggccgg
    34681 attgctggac gatacgcaaa gtgccatcaa tgtggtgcgc catcgcagtg atgtaaaccc
    34741 acaacggctg gtgctgttcg ggcagagtat tggcggggcg aatattcttg ctgttattgg
    34801 tcaggatgat cgtgaaggca tacgtgcggt gatcctcgac tccacatttg cctcttatgc
    34861 aaccatcgcc aatcagatga tccccggcag tggctactta cttgatgaga gttacagcgg
    34921 cgaaaattat atcgccagcg tcagcccgat cccgctttta ctcattcacg gtaaagctga
    34981 tcacgttatc ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag agacaaaacg
    35041 gttgatccta atcccggatg gcgaacacat tgatgctttt tccgatcgtc acggcgatgt
    35101 ttatcgcgaa cagatggtgg actttatcct tagcgcgttg aatccgcaga actaacccat
    35161 gatcgctagc acgataatca ttcacaaaac caccttaaga catgctaatc cactggtcag
    35221 aacagtttaa gatgagaaaa attctgtgac gcttgccaac atttctgatg attagcattc
    35281 ccttcgccat ctccctgagc aaactttagc tattcttatc aattatgctt atgggagatc
    35341 tacagatgat gcctacgctt gctccaccat ctgtcctttc ggctccccag cgccgctgcc
    35401 agatcttgct gacgctcttt cagccggggt tgaccgccac catggcaacc ttcagcgagc
    35461 ttaatggtgt ggatgatgat attgccagtc ttgatatcag cgaaacagga cgggagatcc
    35521 tgcgctatca tcaactcaca ctgacgactg gttatgacgg tagctaccgg gttgaaggta
    35581 cagtgcttaa ccaacgtttg tgtttatttc actggctacg acgtggtttc cgtctgtgtc
    35641 cgtcatttat taccagccaa ttcacccccg ccctgaagag tgaactgaag cggcgcggaa
    35701 ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt agtgaatctc tgctcccgac
    35761 ggctgcaaaa acgctttgaa acgcgcgata ttcatttcct gtgtctgtat ctgcaatatt
    35821 gtttgctgca acaccacgct ggaattacgc ctcagtttaa tccgctccaa cgtcgctggg
    35881 cggaatcctg ccttgaattt caggtagcgc aggaaattgg acgccactgg cagcgtcggg
    35941 cgctccagcc tgtaccgcct gatgagccac tgtttatggc attacttttt tccatgttgc
    36001 gggttcccga tccattgcgt gatgcgcatc agcgagacag acaattgcgt cagtctatca
    36061 aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg tttttatgat gaacaggggt
    36121 tatgcgatca gctttatacc cacctcgccc aggcgttaaa tcgcagtttg tttgccatcg
    36181 gtattgataa taccctgccg gaagagttcg ccagactgta cccacgcctg gtgcgcacca
    36241 cccgcgcggc gctggccgga tttgaaagtg aatacggcgt ccatctttct gatgaggaaa
    36301 gcggtctggt cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat ctgcatgaaa
    36361 aacagattat tctactgacc gggaatgata gcgagcgaga agcgcagatt gagcagcagt
    36421 tacgcgaact aacgttactg ccgctcaaca ttaagcatat gtcggtaaag gcatttttgc
    36481 agacaggcgc tccgcgtggc gcggcactga ttattgcgcc ttataccatg ccattaccgc
    36541 tcttttcacc accgctgatc tatacggacc tgacgctgac aacacatcaa caggagcaga
    36601 tccgcaaaat gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa cattgccgga
    36661 agcgccacca gcgccattac ccagaacacg ccgtggccca gatattgata caggaaaccg
    36721 gcgaaaacgg tcatgatagc gatactgccg cccatcgcga cggcagagta aaccgcctgt
    36781 aaacggatga cttcgctacc ctggcgaaca gcaatatagc gcatggcggc caggtggcag
    36841 accgtgaagg tgccgcaatg caaaatttgc accactatca accacggcaa cgccgtagtt
    36901 gctcccataa tgccccagcg cactacgccg caaatcgccg agatcaacag catatcgcga
    36961 gcactacaac ggcggaaaag tttattactc agcgcaaaga taatgacttc cgccaccacg
    37021 cccagcgacc acaaataccc caccgccgag gccgagtagc cagctgcctg ccagtaaatg
    37081 gcgctaaaac cgtaataggc cgaatgtgcc ccctgcaata aacaaacgca ggccagaaag
    37141 cgccagttct ggcgaaccag cgccaaccac gcagaccaac cggtgctctc ctgctggcga
    37201 cttgcccctt gtggctgaat cgtcggacgg atgagaaagc cgagcagcat ggatgccacg
    37261 cccaacgtca acagcgcgag gatcacccga taatcaaaca tagtgaccag tttgcccgtc
    37321 agcgccgagc caatgacaaa cgccaccgag ccccacagtc gcactttgcc gtaatcaagc
    37381 gggaactgct tttgccacgt attcgccagt gcatcggtca acggtaccag cggtgagaaa
    37441 aagaggttaa agccaatcat caccagcatc agccacgcta cgtgcgcccc cgcccagaag
    37501 gcgacagcaa agagaagtgt cagcagtgcc agcacgcgca aggcggaaat caggcgggaa
    37561 ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac cagacctgcc
    37621 cccaataaca ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca gacgctccag
    37681 aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat agccgagcgc caaccagcgc
    37741 gtggattgca aaaccatgag tccctcccgt caggaggcgt tagtctgggg ttaatcgaat
    37801 gttggtgcaa gttgaaaaat aagaatattc gagcctgctt cccgatgtct gtaacgccag
    37861 ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg caaaaataat gcgtaattat
    37921 gccgttacgc ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa tgccggcttc
    37981 atctcccctt tcttccacgc catcagtaaa gcaatggaag gcacatttcc ggcaatggga
    38041 cgaaaaacaa cctgcccggt attaaaatta ttcatataac cgggtatcaa agtgacgccc
    38101 agccccatgc ccaccagatt catggtcacc agaatattcg ttgccacctg gacgatattt
    38161 ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga accggaatac
    38221 gccggatcgg tactgacgaa attcacgcca tccagttgcg cggcggtgat ctctttttca
    38281 tgtgctaaag ggtgatcaac cggtaacacc accactaatg gttcgtcaaa aagctccaga
    38341 taatcaatct ccgggctata aacaggatgg cgcattaagc cgacgtcgag ttcaccacgg
    38401 cgaatttttt cctcctgttg cgtggtgatt aaactcacca gctcaatcaa ggtgtctggc
    38461 tgtctgagac gaaacattgg taatactttt ggcaataaat tcacttccgc cgatggcaca
    38521 aagccaatgg ttaattgtct gtcttcctga acaattttcc gcgcccgtaa tttggcattt
    38581 tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac attctcccgc cgccgtcaac
    38641 gcgactttgc gcttatccct caccagtaac ggaacaccga cacagttttc aagatcgcgg
    38701 atctggctgc ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg ggtaaagttc
    38761 agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta gttccatcac cttccccttg
    38821 ttatcgaaaa aacgtctcag ctggtagaaa ttaaccattt cacatattag caaccaacca
    38881 gcaacatcct tatgacacaa aaatagaagg tcaatacatc ttatctttca ggattaaaaa
    38941 atatgaccac accctcagat ttaaacattt accaactgat tgatacccaa aatggtcggg
    39001 tcactccgcg tatttatacc gacccagata tttaccaact ggagcttgaa cgtattttcg
    39061 gccgttgctg gttatttctc gcccacgaaa gccagatccc aaaacctggt gatttcttta
    39121 acacctacat gggggaagat gcggttgtcg tagtgcgtca gaaagacggc agcattaagg
    39181 cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat tgcggcaaca
    39241 ctcgcgcctt tacctgtccg tatcacggct ggtcttatgg cattaacggc gagttgatcg
    39301 atgtaccgct ggaacctcgc gcctacccac aagggttgtg taaatcccac tggggactaa
    39361 acgaagttcc ttgtgtggag agttataaag ggctgatttt tggcaactgg gataccagcg
    39421 caccgggcct gcgtgattac ctgggtgaca ttgcctggta tctggatggc atgctggatc
    39481 gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa gtgggtgatc aactgtaact
    39541 ggaaattccc ggcagagcag ttcgccagtg accagtatca tgctctgttc agccatgctt
    39601 ctgccgttca ggtattaggg gcgaaagatg atggcagcga taagcgcctc ggtgatggac
    39661 aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct gcaatttggt caggacggtc
    39721 acggtagcgg tttcttcttt actgaaaaac cggatgctaa tgtctgggtc gatggcgcag
    39781 tttcaagcta ttaccgcgaa acctatgccg aagcagaaca acgtttaggt gaagttcgcg
    39841 ccctgcgcct ggcgggtcat aacaatattt tccccacgct ttcatggctc aacggcactg
    39901 ccacgctccg cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg gcgttctgta
    39961 ttactgacaa agccgcctcc gatgaagtta aagccgcttt tgaaaacagc gccactcgtg
    40021 cttttggtcc tgctggtttt ctcgagcagg atgactcgga gaactggtgt gaaatccaga
    40081 aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg tctggaaatg gggcttggtc
    40141 aggaaaagcg ccgcgacgac ggcattcctg gcattactaa ctatatcttt tcagaaactg
    40201 ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag tagcgaaagc tggcaggaag
    40261 tgctcgataa aaccgccgct taccagcagg aggtgatgaa atgagtgcgc aagtttcact
    40321 agagttacat caccgcatta gccagtttct ctttcacgaa gccagcttac tggacgactg
    40381 gaaatttcgt gactggctgg agcagctcga caaagagatt cgttacacca tgcgcaccac
    40441 agttaacgcg caaacacgcg accgccgcaa aggcgtccag ccaccgacaa cctggatttt
    40501 taatgacacc aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg gcatggcctg
    40561 ggcagaagag ccgccgtcac gcacccgtca cttaatcagc aactgccaga taagcgaaac
    40621 cgacatccca aacgtatttg ctgtgcgggt aaattatctg ctttatcggg cacaaaaaga
    40681 gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa gttcgccgtc tggaagatga
    40741 caactggcgc ttgctggaac gggatatcgt cctggatcaa gcggtaatca cttcccataa
    40801 cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga tgtgccggag
    40861 ggtgaggctc tccggatcga tacctcgccc gtcatcgccc tgttcaacgt tggcggcgag
    40921 ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt cgatgtcaga agggtatctg
    40981 gaagatgacg ccacggtgga gtgcccgcta cacgccgcca gtttttgcct gaaaacgggg
    41041 aaagcgttat gcctgcccgc caccgatccg ctcaccactt atccagtaca cgttgaaggt
    41101 ggtgacattt tcatcgactt accggaggcg cagccatgag cgatctgcat aacgagtcca
    41161 tttttattac cggcggcgga tcgggattag ggctggcgct ggtcgagcga tttatcgaag
    41221 aaggcgcgca agttgccacg ctggaactgt cggcggcaaa agtcgccagt ctgcgtcagc
    41281 gatttggcga acatattctg gcggtggaag gtaacgtgac ctgttatgcc gattatcaac
    41341 gcgcggtcga tcagatcctg actcgttccg gcaagctgga ttgttttatc ggcaatgcag
    41401 gcatctggga tcacaatgcc tcactggtta atactcccgc agagacgctc gaaaccggct
    41461 tccacgagct gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa gcctgcgctc
    41521 cggcgttaat cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc gccaggtatc
    41581 ctggcggcgg tggcccgctg tacaccgcca gtaaacatgc cgcaaccgga cttattcgcc
    41641 aactggctta tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg tgtggtatgg
    41701 ccagcgacct gcgcggccca caggcgctcg ggcaaagtga aacctcgata atgcagtctc
    41761 tgacgccgga gaaaattgcc gccattttac cgctgcaatt tttcccgcaa ccggcggatt
    41821 ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta agcggtgtga
    41881 tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg gctgggctgg
    41941 atctttaagg aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc gggcaagcgg
    42001 cggcaatggc tgcggcctcg ctacgccagc aagggttcac cggtgagctg catctgtttt
    42061 ccgatgagcg acatcttcct tatgaacgac ctccactctc gaaatccatg ttgctggaag
    42121 attccccgca gttacagcag gtgttacccg ctaactggtg gcaggaaaac aatgttcatc
    42181 tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta gtgttaacca
    42241 acggcgaaag ctggcactgg gatcagcttt ttatagcaat cggcgcggca gctcgaccgc
    42301 tgccgttgct tgatgcactg ggagaacgct gctttaccct acgccatgcc ggtgatgccg
    42361 ccagactgcg agaagttctg cagcccgaac ggtcagtcgt gattgtcggt gccggaacta
    42421 ttggtctgga actggctgcc agcgccacgc agcgcagatg taaggtgaca gtgattgaac
    42481 tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc
    42541 gccaccagca ggctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg
    42601 gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt
    42661 acggtattgg tatcagcgcc aacgagcaac tggctcgcga ggccaacctt gatactgcca
    42721 atggcattgt cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg
    42781 atgtggcaat cactcgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg
    42841 ccaataacca ggcgcaaatt gccgctgccg caatgttagg gctaccgcta ccgctactgc
    42901 cgccgccgtg gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc
    42961 gtggcgatga ctggctttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc
    43021 tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca ggggcgtgag attcgcccaa
    43081 ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca
    43141 tcgcgcttaa atcactgtaa ccaggataat tagcgaatat ctcaacgcct ggggcgtggc
    43201 gaggtgcaag agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga
    43261 acacactacg t
//
LOCUS       DAZOYS010000014        43121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOYS010000014 DAZOYS010000000
VERSION     DAZOYS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.014"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUN9_000641"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUN9_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0694143.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUN9_000642"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUN9_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0694144.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUN9_000643"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUN9_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0694145.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUN9_000644"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUN9_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0694146.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUN9_000645"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUN9_000645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0694147.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUN9_000646"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUN9_000646"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0694148.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUN9_000647"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUN9_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0694149.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUN9_000648"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUN9_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0694150.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUN9_000649"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUN9_000649"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0694151.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUN9_000650"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUN9_000650"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0694152.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUN9_000651"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUN9_000651"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0694153.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUN9_000652"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUN9_000652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0694154.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUN9_000653"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUN9_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0694155.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUN9_000654"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUN9_000654"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0694156.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUN9_000655"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUN9_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0694157.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUN9_000656"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUN9_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0694158.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUN9_000657"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUN9_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0694159.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUN9_000658"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUN9_000658"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0694160.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUN9_000659"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUN9_000659"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0694161.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUN9_000660"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUN9_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0694162.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUN9_000661"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUN9_000661"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0694163.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUN9_000662"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUN9_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0694164.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUN9_000663"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUN9_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0694165.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUN9_000664"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUN9_000664"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0694166.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHUN9_000665"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHUN9_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUN9_000666"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUN9_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0694167.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHUN9_000667"
     CDS             complement(31717..33003)
                     /locus_tag="ACHUN9_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0694168.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHUN9_000668"
     CDS             complement(33054..33995)
                     /locus_tag="ACHUN9_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0694169.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHUN9_000669"
     CDS             complement(34010..34780)
                     /locus_tag="ACHUN9_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0694170.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35252..36766
                     /gene="caiT"
                     /locus_tag="ACHUN9_000670"
     CDS             35252..36766
                     /gene="caiT"
                     /locus_tag="ACHUN9_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0694171.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36797..37939
                     /gene="caiA"
                     /locus_tag="ACHUN9_000671"
     CDS             36797..37939
                     /gene="caiA"
                     /locus_tag="ACHUN9_000671"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0694172.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38068..39285
                     /gene="caiB"
                     /locus_tag="ACHUN9_000672"
                     /pseudo
     CDS             38068..39285
                     /gene="caiB"
                     /locus_tag="ACHUN9_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            39359..40912
                     /gene="caiC"
                     /locus_tag="ACHUN9_000673"
     CDS             39359..40912
                     /gene="caiC"
                     /locus_tag="ACHUN9_000673"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0694173.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            41021..41806
                     /gene="caiD"
                     /locus_tag="ACHUN9_000674"
     CDS             41021..41806
                     /gene="caiD"
                     /locus_tag="ACHUN9_000674"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0694174.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41812..42402
                     /gene="caiE"
                     /locus_tag="ACHUN9_000675"
     CDS             41812..42402
                     /gene="caiE"
                     /locus_tag="ACHUN9_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0694175.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42611..43006)
                     /gene="caiF"
                     /locus_tag="ACHUN9_000676"
     CDS             complement(42611..43006)
                     /gene="caiF"
                     /locus_tag="ACHUN9_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0694176.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        10345 a        11740 c        11339 g         9697 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaatgtg
    35101 ataacaatca cagaatgcag cttattgaat acccattatg agttagccat taacgcgtcc
    35161 acgaggttaa taataattat atcaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa
    35221 taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat
    35281 agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt ggcttacagt
    35341 cagagatctg gatgcagcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt
    35401 atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt tctggctggt
    35461 gtttggcccg tatgccaaaa agcgtttagg taacgaacca cctgaattta gtaccgccag
    35521 ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct ggggatcgat
    35581 tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact cgacaggggc
    35641 gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta
    35701 cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg
    35761 ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggcac
    35821 tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct
    35881 tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca
    35941 actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg tcgcttgcgg
    36001 tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tcctgatgct
    36061 gggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca ccgattcggt
    36121 ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca tcgctaaagg
    36181 cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt atgctatcca
    36241 gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac tgtgcttcgg
    36301 catggtgctg gggctgacag cgtcaacctg gatcctgtgg actgtactcg gtagtaacac
    36361 tctgctgttg atagataaaa acatcatcaa cattccaaat ctgatcgaac agtacggtgt
    36421 ggcgcgcgcc atcattgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg
    36481 cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct
    36541 ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccacctctgc tggtgcgtat
    36601 cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg gcggcctgaa
    36661 accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca acattatggt
    36721 gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat
    36781 atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg
    36841 tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt gcgaccgtga
    36901 cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg acagtctgct
    36961 gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat
    37021 ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg gcgggttcaa
    37081 caccttcctg cgcgaaggca cacaagagca gatcgacaaa attatggctt tccgcggcac
    37141 cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg acgtgggtag
    37201 cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt
    37261 tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc
    37321 ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac
    37381 caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct ttgacgacgt
    37441 ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga
    37501 agagttcgac catgaacgtt tcctggtagc cctcaccaac tacggtacgg cgatgtgcgc
    37561 ttttgaagat gcggcgcgct acgccaatca gcgcgtgcag tttggcgagg ctattggtcg
    37621 tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa
    37681 catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc
    37741 gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtt gtggatagcg caatgcaggt
    37801 gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgatctgcg
    37861 tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg gtcgcgcggt
    37921 gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc gtcttatcag
    37981 gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg gcagtcatgc
    38041 gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa attcgggccg
    38101 ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt cgccgggcaa
    38161 atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgccta ggccgacacc
    38221 attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc gctgtcgtta
    38281 aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac caccgatatc
    38341 ttcatcgaag ccagcaaagg tccggccttt gcccgtcgtg gcattaccga tgaagtactg
    38401 tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca gtacggcacc
    38461 gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag tggttacctg
    38521 attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc cgattacttt
    38581 tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg tgaaaccggt
    38641 aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg ccagtacttc
    38701 atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg taaagatccc
    38761 tactacgccg gttgcggtct gtataaatgc gccgacggct acatcgtgat ggaactggtg
    38821 ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct actcggtacg
    38881 ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta cggcccactg
    38941 gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt taaagaacgc
    39001 tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact ggaaagcaat
    39061 ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg tcgcacctgc
    39121 aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg gcgcggaatg
    39181 ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag cgaaaacgac
    39241 attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg tggcgtcagt
    39301 cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata gaggtgcaat
    39361 ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg acgtttacgg
    39421 tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt atagttatct
    39481 tgagttaaat caggagatta accgcacgac aaacctgttt tatacgctgg ggattcgcaa
    39541 aggcgacaag gttgctctac atctcgacaa ctgcccggaa tttatctttt gctggttcgg
    39601 gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttgc gcgaggaaag
    39661 cgcgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc aattctatcc
    39721 tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt gcctgacaga
    39781 tgtggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa atcaacaacc
    39841 tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa ttctcttcac
    39901 ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc tgcgcttcgc
    39961 tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctacc tgacggtaat
    40021 gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt ctgccggggc
    40081 cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg tacagaagta
    40141 ccgcgccacc gttaccgaat gtattccgat gatgatccgt accttgatgg cgcagccgcc
    40201 ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca acttgtcgga
    40261 gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt cttatgggat
    40321 gacggaaacc attgtgggca ttatcggcga tcgccctggc gataaacgac gctggccgtc
    40381 gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca atcgcccgct
    40441 cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa ccatcttcaa
    40501 agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg gctggctaca
    40561 taccggcgat accggatact gcgacgaaga gggctttttt tatttcgtcg atcgccgctg
    40621 caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa atatcatcgc
    40681 cacccatcca aaaattcagg atatcgtggt tgtgggtatt aaagattcga ttcgcgatga
    40741 agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag aggaattttt
    40801 ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg agatcagaaa
    40861 agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat aaatgaaaca
    40921 gcagggaact accctgccaa ctaataatca cgctataaaa caatatgcat tttttgcagg
    40981 gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat cattacatct
    41041 gacccgcaat ggatcaattc tggaaattac ccttgatcgt ccaaaagcga atgctattga
    41101 tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg atccgcaatt
    41161 acgtgtcgcc attattaccg gtgccggaga gaagttcttt tccgcgggct gggatttaaa
    41221 agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggat ttgcgggatt
    41281 aaccgaaatt ttcaatctcg acaaaccggt tatcgcagct gtgaacggct atgcctttgg
    41341 cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata acgccagctt
    41401 cgccctgccg gaagccaagc tgggcatcgt tcctgacagc ggcggcgtgc tgcgtctacc
    41461 gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac gaatgggcgc
    41521 agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg aactgatgga
    41581 taacgcccgc gaactggcgc agcagctggt taacagcgcc ccgctggcga ttgcggcgct
    41641 gaaagagatc ttccgcacca ccagcgaaat gccggtagaa gaagcgtatc gctatattcg
    41701 cagcggcgtg ttgaaacact atccgtcggt tctgcattcg gaggatgcca ttgaagggcc
    41761 gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac cgtgagctat
    41821 tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca tcccagtgcc
    41881 gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact cgcctcactg
    41941 cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga tggctgcatt
    42001 atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat cgggcacgga
    42061 gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa cagcgtgatt
    42121 atggatggcg cggtcattgg cgaagagagc attgttgccg ccatgagctt tgtcaaagca
    42181 ggctttcacg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc tgtacgcagt
    42241 gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca ggatcttgtc
    42301 gggcgctgtc atgcatcgtt acatgaaaca cagccactga ggcaaatgga ggaaaatcgc
    42361 ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg gatgcggcgt
    42421 aaacgcctta tccaactctg ctggagagca accgtaggcc ggataagatg cgccagcatc
    42481 gcatccggcg atagctgtcg gatgcggcat aaacgcctta tccaacccgg ctggagagca
    42541 accgtaggct ggataagatg cgccagcatt gcatccagca accaggctgc gtggtatcaa
    42601 cgaaatgaca ttaacgacgc atactctttg acaacatcat ctgccacttc tgctcgcggt
    42661 taagttccgt gagcggaacg gcaggaatac gcggcgtctt tcttacgccg actaatttat
    42721 cccggctgtt attgcgcagg cgcgcgtaaa tttgctcatc gatatcgacc acttttacca
    42781 gtcgctggca ctggcatccc cgtccttcca gcttattagg gatcatctta actgcgcagc
    42841 ttatttctgc gacttccgac agaatataag tcagggtatt aaccgccttg ctgtgttcaa
    42901 tatcgaaatg aatagagatc tctcttgcta tcacccaccg attttcagcc atcatccatt
    42961 cggcgattaa caagtagagt ggtttttcaa catatccttc acacatacga actccagttt
    43021 cacaaattaa aagtctggct atgactcgtt ttgaagcaat taaacaaggc catattaaaa
    43081 ttgtggatat atcgatagta tgcaacttca ccgtcgataa g
//
LOCUS       DAZOYS010000015        41011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOYS010000015 DAZOYS010000000
VERSION     DAZOYS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.015"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..262
                     /locus_tag="ACHUN9_000677"
     CDS             <1..262
                     /locus_tag="ACHUN9_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694177.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="ACHUN9_000678"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="ACHUN9_000678"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0694178.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="ACHUN9_000679"
     tRNA            2260..2336
                     /locus_tag="ACHUN9_000679"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="ACHUN9_000680"
     tRNA            2345..2429
                     /locus_tag="ACHUN9_000680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="ACHUN9_000681"
     tRNA            2453..2527
                     /locus_tag="ACHUN9_000681"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="ACHUN9_000682"
     tRNA            2562..2636
                     /locus_tag="ACHUN9_000682"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="ACHUN9_000683"
     tRNA            2652..2728
                     /locus_tag="ACHUN9_000683"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="ACHUN9_000684"
     tRNA            2777..2851
                     /locus_tag="ACHUN9_000684"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="ACHUN9_000685"
     tRNA            2888..2962
                     /locus_tag="ACHUN9_000685"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHUN9_000686"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="ACHUN9_000686"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0694179.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="ACHUN9_000687"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="ACHUN9_000687"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0694180.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="ACHUN9_000688"
     CDS             6014..7054
                     /locus_tag="ACHUN9_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0694181.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHUN9_000689"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="ACHUN9_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0694182.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="ACHUN9_000690"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="ACHUN9_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0694183.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="ACHUN9_000691"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="ACHUN9_000691"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0694184.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="ACHUN9_000692"
     CDS             complement(10127..12004)
                     /locus_tag="ACHUN9_000692"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0694185.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="ACHUN9_000693"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="ACHUN9_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0694186.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHUN9_000694"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="ACHUN9_000694"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0694187.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="ACHUN9_000695"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="ACHUN9_000695"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0694188.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGMQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="ACHUN9_000696"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="ACHUN9_000696"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0694189.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="ACHUN9_000697"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="ACHUN9_000697"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0694190.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="ACHUN9_000698"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="ACHUN9_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0694191.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="ACHUN9_000699"
     CDS             complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="ACHUN9_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0694192.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19999..20706)
                     /locus_tag="ACHUN9_000700"
     CDS             complement(19999..20706)
                     /locus_tag="ACHUN9_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0694193.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20808..21362
                     /locus_tag="ACHUN9_000701"
     CDS             20808..21362
                     /locus_tag="ACHUN9_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0694194.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21372..22799)
                     /locus_tag="ACHUN9_000702"
     CDS             complement(21372..22799)
                     /locus_tag="ACHUN9_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0694195.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22796..23503)
                     /locus_tag="ACHUN9_000703"
     CDS             complement(22796..23503)
                     /locus_tag="ACHUN9_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0694196.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23667..24644
                     /gene="ybeQ"
                     /locus_tag="ACHUN9_000704"
     CDS             23667..24644
                     /gene="ybeQ"
                     /locus_tag="ACHUN9_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR0694197.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24714..25196)
                     /locus_tag="ACHUN9_000705"
     CDS             complement(24714..25196)
                     /locus_tag="ACHUN9_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0694198.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25431..28013
                     /gene="leuS"
                     /locus_tag="ACHUN9_000706"
     CDS             25431..28013
                     /gene="leuS"
                     /locus_tag="ACHUN9_000706"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0694199.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28028..28609
                     /gene="lptE"
                     /locus_tag="ACHUN9_000707"
     CDS             28028..28609
                     /gene="lptE"
                     /locus_tag="ACHUN9_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0694200.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28609..29640
                     /gene="holA"
                     /locus_tag="ACHUN9_000708"
     CDS             28609..29640
                     /gene="holA"
                     /locus_tag="ACHUN9_000708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0694201.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29642..30283
                     /gene="nadD"
                     /locus_tag="ACHUN9_000709"
     CDS             29642..30283
                     /gene="nadD"
                     /locus_tag="ACHUN9_000709"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0694202.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30307..30918
                     /locus_tag="ACHUN9_000710"
                     /pseudo
     CDS             30307..30918
                     /locus_tag="ACHUN9_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31178..31495
                     /gene="rsfS"
                     /locus_tag="ACHUN9_000711"
     CDS             31178..31495
                     /gene="rsfS"
                     /locus_tag="ACHUN9_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0694203.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31499..31966
                     /gene="rlmH"
                     /locus_tag="ACHUN9_000712"
     CDS             31499..31966
                     /gene="rlmH"
                     /locus_tag="ACHUN9_000712"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0694204.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31997..33898
                     /gene="mrdA"
                     /locus_tag="ACHUN9_000713"
     CDS             31997..33898
                     /gene="mrdA"
                     /locus_tag="ACHUN9_000713"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0694205.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33901..35013
                     /gene="mrdB"
                     /locus_tag="ACHUN9_000714"
                     /gene_synonym="rodA"
     CDS             33901..35013
                     /gene="mrdB"
                     /locus_tag="ACHUN9_000714"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0694206.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35024..36112
                     /gene="rlpA"
                     /locus_tag="ACHUN9_000715"
     CDS             35024..36112
                     /gene="rlpA"
                     /locus_tag="ACHUN9_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0694207.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36251..37462
                     /gene="dacA"
                     /locus_tag="ACHUN9_000716"
     CDS             36251..37462
                     /gene="dacA"
                     /locus_tag="ACHUN9_000716"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0694208.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37572..37835
                     /gene="ybeD"
                     /locus_tag="ACHUN9_000717"
     CDS             37572..37835
                     /gene="ybeD"
                     /locus_tag="ACHUN9_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0694209.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37936..38577
                     /gene="lipB"
                     /locus_tag="ACHUN9_000718"
     CDS             37936..38577
                     /gene="lipB"
                     /locus_tag="ACHUN9_000718"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0694210.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38837..39790
                     /locus_tag="ACHUN9_000719"
     CDS             38837..39790
                     /locus_tag="ACHUN9_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0694211.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39999..40964
                     /gene="lipA"
                     /locus_tag="ACHUN9_000720"
     CDS             39999..40964
                     /gene="lipA"
                     /locus_tag="ACHUN9_000720"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0694212.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10295 a        10448 c        10294 g         9974 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagcccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cgtaaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagttt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc ggtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacgg cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctga cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtatgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    19561 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    19621 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    19681 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    19741 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    19801 tggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    19861 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    19921 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    19981 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    20041 ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    20101 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    20161 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    20221 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    20281 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg acagaaaccc
    20341 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    20401 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    20461 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    20521 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    20581 tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg cgggtttgca
    20641 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    20701 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    20761 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    20821 caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    20881 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    20941 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    21001 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    21061 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    21121 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    21181 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    21241 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    21301 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    21361 aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc aaagataaaa
    21421 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt
    21481 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    21541 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    21601 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    21661 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    21721 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    21781 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagctgt
    21841 ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa cgggtaataa
    21901 tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt ggagggatac
    21961 tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca atagacgcgt
    22021 tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    22081 gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg gagtaaattt
    22141 ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc
    22201 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    22261 ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    22321 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    22381 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    22441 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    22501 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    22561 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    22621 acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg acgaagttgt
    22681 ttaaaaccct gcggatcggt ttctggatga aaggacggta acagcgccag ataagcgcgg
    22741 cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca atttttcata
    22801 ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    22861 acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    22921 aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    22981 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    23041 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    23101 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    23161 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    23221 cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa cgaaaccagc
    23281 ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg tgaattgtgc
    23341 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    23401 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    23461 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    23521 attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt ttctgacgca
    23581 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt
    23641 ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat
    23701 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    23761 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    23821 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    23881 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    23941 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag
    24001 aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
    24061 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
    24121 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
    24181 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg
    24241 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    24301 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    24361 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    24421 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
    24481 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    24541 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    24601 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
    24661 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    24721 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
    24781 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    24841 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
    24901 agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc
    24961 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    25021 aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
    25081 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    25141 aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    25201 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    25261 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
    25321 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    25381 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    25441 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    25501 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    25561 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    25621 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
    25681 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    25741 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    25801 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    25861 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    25921 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    25981 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    26041 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    26101 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacatgctga
    26161 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    26221 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    26281 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    26341 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    26401 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    26461 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
    26521 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    26581 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    26641 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    26701 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    26761 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    26821 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    26881 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    26941 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    27001 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    27061 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    27121 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    27181 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    27241 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    27301 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    27361 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    27421 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
    27481 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    27541 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    27601 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
    27661 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
    27721 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
    27781 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    27841 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca acggaagaac
    27901 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
    27961 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    28021 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
    28081 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
    28141 ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt tacgtctgaa
    28201 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    28261 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    28321 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    28381 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
    28441 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    28501 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    28561 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
    28621 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    28681 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
    28741 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
    28801 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    28861 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    28921 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    28981 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    29041 gctcagcttc cccgctgggt tgctgcgcgc gcaaaacagc tcaacttaga actggatgac
    29101 gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    29161 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
    29221 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    29281 aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt
    29341 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    29401 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    29461 atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc
    29521 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    29581 aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    29641 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    29701 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    29761 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    29821 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    29881 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    29941 gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct ggtacgaata
    30001 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    30061 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    30121 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    30181 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    30241 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    30301 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt
    30361 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
    30421 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
    30481 cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa
    30541 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    30601 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
    30661 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
    30721 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
    30781 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta ggggtgttgg
    30841 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    30901 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    30961 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
    31021 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    31081 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    31141 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    31201 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    31261 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    31321 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    31381 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    31441 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    31501 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    31561 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    31621 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    31681 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    31741 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
    31801 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
    31861 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    31921 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    31981 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    32041 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    32101 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
    32161 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    32221 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
    32281 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
    32341 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    32401 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    32461 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    32521 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    32581 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
    32641 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    32701 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    32761 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    32821 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    32881 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg ccccgaatac
    32941 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    33001 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    33061 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
    33121 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    33181 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    33241 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    33301 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
    33361 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    33421 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    33481 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    33541 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
    33601 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    33661 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    33721 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    33781 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    33841 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    33901 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    33961 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    34021 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct agtcatcatg
    34081 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    34141 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    34201 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    34261 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    34321 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    34381 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    34441 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    34501 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc ggaatcagac
    34561 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    34621 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    34681 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    34741 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    34801 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    34861 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    34921 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    34981 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    35041 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    35101 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    35161 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    35221 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    35281 tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    35341 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    35401 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg accgcgttat
    35461 ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    35521 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    35581 agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg gcgcgggaac
    35641 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    35701 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    35761 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    35821 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    35881 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    35941 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    36001 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    36061 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    36121 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    36181 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    36241 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    36301 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    36361 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    36421 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    36481 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    36541 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    36601 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    36661 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    36721 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    36781 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    36841 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    36901 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    36961 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    37021 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    37081 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    37141 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    37201 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    37261 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    37321 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    37381 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    37441 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    37501 actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    37561 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    37621 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    37681 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    37741 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    37801 taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcccct
    37861 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    37921 cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    37981 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    38041 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    38101 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    38161 acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    38221 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    38281 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    38341 atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac
    38401 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    38461 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta
    38521 ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg
    38581 catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    38641 atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc tacgccttgc
    38701 tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    38761 tccgttccaa ttagacataa gattattgta atttttttaa atattatttt tccatgaata
    38821 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    38881 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    38941 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    39001 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    39061 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    39121 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    39181 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    39241 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    39301 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    39361 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    39421 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    39481 actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    39541 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    39601 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    39661 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    39721 ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg ttatccgtaa
    39781 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    39841 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    39901 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    39961 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    40021 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    40081 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    40141 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    40201 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    40261 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    40321 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    40381 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    40441 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    40501 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    40561 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    40621 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    40681 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    40741 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    40801 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    40861 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    40921 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    40981 tacatctgtc tgataaaaaa cccgcttttt g
//
LOCUS       DAZOYS010000016        40106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOYS010000016 DAZOYS010000000
VERSION     DAZOYS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.016"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUN9_000721"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUN9_000721"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0694213.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUN9_000722"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUN9_000722"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0694214.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUN9_000723"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUN9_000723"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0694215.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUN9_000724"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUN9_000724"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0694216.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUN9_000725"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUN9_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0694217.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUN9_000726"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUN9_000726"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0694218.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUN9_000727"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUN9_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0694219.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUN9_000728"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUN9_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0694220.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUN9_000729"
     CDS             6537..6773
                     /locus_tag="ACHUN9_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0694221.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUN9_000730"
     CDS             6776..7633
                     /locus_tag="ACHUN9_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0694222.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUN9_000731"
     CDS             7654..9645
                     /locus_tag="ACHUN9_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0694223.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUN9_000732"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUN9_000732"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0694224.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUN9_000733"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUN9_000733"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0694225.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUN9_000734"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUN9_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0694226.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUN9_000735"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUN9_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0694227.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUN9_000736"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUN9_000736"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0694228.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUN9_000737"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUN9_000737"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0694229.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUN9_000738"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUN9_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0694230.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUN9_000739"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUN9_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUN9_000740"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUN9_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0694231.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUN9_000741"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUN9_000741"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0694232.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUN9_000742"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUN9_000742"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0694233.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHUN9_000743"
     CDS             complement(21570..22739)
                     /locus_tag="ACHUN9_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694234.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUN9_000744"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUN9_000744"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0694235.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHUN9_000745"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHUN9_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0694236.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUN9_000746"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUN9_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0694237.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHUN9_000747"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHUN9_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0694238.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUN9_000748"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUN9_000748"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0694239.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUN9_000749"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUN9_000749"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0694240.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUN9_000750"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUN9_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0694241.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHUN9_000751"
     CDS             complement(30320..31576)
                     /locus_tag="ACHUN9_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0694242.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHUN9_000752"
     tRNA            31884..31960
                     /locus_tag="ACHUN9_000752"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHUN9_000753"
     tRNA            31965..32041
                     /locus_tag="ACHUN9_000753"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHUN9_000754"
     CDS             32149..32454
                     /locus_tag="ACHUN9_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0694243.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHUN9_000755"
     CDS             32580..34184
                     /locus_tag="ACHUN9_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0694244.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUN9_000756"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUN9_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0694245.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUN9_000757"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUN9_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0694246.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHUN9_000758"
     CDS             complement(35794..36462)
                     /locus_tag="ACHUN9_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0694247.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHUN9_000759"
     CDS             complement(36526..37164)
                     /locus_tag="ACHUN9_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0694248.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHUN9_000760"
     CDS             complement(37177..39279)
                     /locus_tag="ACHUN9_000760"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0694249.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHUN9_000761"
     CDS             complement(39300..39926)
                     /locus_tag="ACHUN9_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0694250.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9623 a        10542 c         9886 g        10055 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccaa
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg gcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgt
//
LOCUS       DAZOYS010000017        39888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOYS010000017 DAZOYS010000000
VERSION     DAZOYS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.017"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(126..801)
                     /locus_tag="ACHUN9_000762"
                     /pseudo
     CDS             complement(126..801)
                     /locus_tag="ACHUN9_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(946..1554)
                     /locus_tag="ACHUN9_000763"
     CDS             complement(946..1554)
                     /locus_tag="ACHUN9_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0694251.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1738..2055)
                     /gene="metJ"
                     /locus_tag="ACHUN9_000764"
     CDS             complement(1738..2055)
                     /gene="metJ"
                     /locus_tag="ACHUN9_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0694252.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2332..3492
                     /gene="metB"
                     /locus_tag="ACHUN9_000765"
     CDS             2332..3492
                     /gene="metB"
                     /locus_tag="ACHUN9_000765"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0694253.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3495..5927
                     /gene="metL"
                     /locus_tag="ACHUN9_000766"
     CDS             3495..5927
                     /gene="metL"
                     /locus_tag="ACHUN9_000766"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0694254.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6066..6200)
                     /locus_tag="ACHUN9_000767"
     CDS             complement(6066..6200)
                     /locus_tag="ACHUN9_000767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694255.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6276..7166
                     /gene="metF"
                     /locus_tag="ACHUN9_000768"
     CDS             6276..7166
                     /gene="metF"
                     /locus_tag="ACHUN9_000768"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0694256.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7495..9675
                     /gene="katG"
                     /locus_tag="ACHUN9_000769"
     CDS             7495..9675
                     /gene="katG"
                     /locus_tag="ACHUN9_000769"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0694257.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9769..10674
                     /gene="yijE"
                     /locus_tag="ACHUN9_000770"
     CDS             9769..10674
                     /gene="yijE"
                     /locus_tag="ACHUN9_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0694258.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10701..11318)
                     /locus_tag="ACHUN9_000771"
     CDS             complement(10701..11318)
                     /locus_tag="ACHUN9_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0694259.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11592..12695)
                     /gene="gldA"
                     /locus_tag="ACHUN9_000772"
     CDS             complement(11592..12695)
                     /gene="gldA"
                     /locus_tag="ACHUN9_000772"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0694260.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAVGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12706..13368)
                     /gene="fsa"
                     /locus_tag="ACHUN9_000773"
     CDS             complement(12706..13368)
                     /gene="fsa"
                     /locus_tag="ACHUN9_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0694261.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13380..15881)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_000774"
     CDS             complement(13380..15881)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_000774"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0694262.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16190..17269
                     /locus_tag="ACHUN9_000775"
     CDS             16190..17269
                     /locus_tag="ACHUN9_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0694263.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17284..17604
                     /locus_tag="ACHUN9_000776"
                     /pseudo
     CDS             17284..17604
                     /locus_tag="ACHUN9_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17655..19951
                     /locus_tag="ACHUN9_000777"
                     /pseudo
     CDS             17655..19951
                     /locus_tag="ACHUN9_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19917..20795
                     /locus_tag="ACHUN9_000778"
     CDS             19917..20795
                     /locus_tag="ACHUN9_000778"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0694264.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20797..21138
                     /locus_tag="ACHUN9_000779"
     CDS             20797..21138
                     /locus_tag="ACHUN9_000779"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0694265.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21125..21976)
                     /locus_tag="ACHUN9_000780"
     CDS             complement(21125..21976)
                     /locus_tag="ACHUN9_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0694266.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22191..23924)
                     /locus_tag="ACHUN9_000781"
     CDS             complement(22191..23924)
                     /locus_tag="ACHUN9_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0694267.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24107..26758)
                     /gene="ppc"
                     /locus_tag="ACHUN9_000782"
     CDS             complement(24107..26758)
                     /gene="ppc"
                     /locus_tag="ACHUN9_000782"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0694268.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27110..28261)
                     /gene="argE"
                     /locus_tag="ACHUN9_000783"
     CDS             complement(27110..28261)
                     /gene="argE"
                     /locus_tag="ACHUN9_000783"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0694269.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28415..29419
                     /gene="argC"
                     /locus_tag="ACHUN9_000784"
     CDS             28415..29419
                     /gene="argC"
                     /locus_tag="ACHUN9_000784"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0694270.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29427..30203
                     /gene="argB"
                     /locus_tag="ACHUN9_000785"
     CDS             29427..30203
                     /gene="argB"
                     /locus_tag="ACHUN9_000785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0694271.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30264..31637
                     /gene="argH"
                     /locus_tag="ACHUN9_000786"
     CDS             30264..31637
                     /gene="argH"
                     /locus_tag="ACHUN9_000786"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0694272.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31904..32821
                     /gene="oxyR"
                     /locus_tag="ACHUN9_000787"
     CDS             31904..32821
                     /gene="oxyR"
                     /locus_tag="ACHUN9_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0694273.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32804..34204)
                     /gene="sthA"
                     /locus_tag="ACHUN9_000788"
     CDS             complement(32804..34204)
                     /gene="sthA"
                     /locus_tag="ACHUN9_000788"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0694274.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34525..35175
                     /gene="fabR"
                     /locus_tag="ACHUN9_000789"
     CDS             34525..35175
                     /gene="fabR"
                     /locus_tag="ACHUN9_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0694275.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35175..35534
                     /locus_tag="ACHUN9_000790"
     CDS             35175..35534
                     /locus_tag="ACHUN9_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0694276.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35574..36674)
                     /gene="trmA"
                     /locus_tag="ACHUN9_000791"
     CDS             complement(35574..36674)
                     /gene="trmA"
                     /locus_tag="ACHUN9_000791"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0694277.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36788..36978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37043..38887
                     /gene="btuB"
                     /locus_tag="ACHUN9_000792"
     CDS             37043..38887
                     /gene="btuB"
                     /locus_tag="ACHUN9_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0694278.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38832..39689
                     /gene="murI"
                     /locus_tag="ACHUN9_000793"
     CDS             38832..39689
                     /gene="murI"
                     /locus_tag="ACHUN9_000793"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0694279.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9104 a        10031 c        11002 g         9751 t
ORIGIN      
        1 aatatatgtc atttgagaaa aaagagcaga atgaggtaaa aatctttggc tgtgtattca
       61 gtctggtaat catccaagga aagcttatca gaagggtgat gactccaatt agtagaacat
      121 atattttcga taaaagcacc gatgactttt tcatgaagtt ctacagaacg cgagaaacag
      181 atggttctgc gggccagacg tttgattcgg gttctcaggg tcagattgtt acgctcaatg
      241 tgctgagtaa aaatcttacc agtcagatgc ttctccttcg gtaactctct agcgtaacta
      301 ccccattcgt cagtggtcaa catgccgatg gtaaaagggt aagcagcgcc cgcaattcac
      361 gacaggtttc gtcagtccgg gggccaaaag tgtaggccag aacaccacca gttttcgtgt
      421 tgtacacgta ccagagccag tgctgtcgcg ctttactgcc gacaaagctc cattgctgac
      481 taagtttgca gatgagtgcc acatcagcat gagcgaccgg ggatgacgtt attcttcgtg
      541 gcgagagttt tttaaagttc ggatgactgt attaatgccg attttcagcg tacgggcagt
      601 atcgcgaacc ccggcaccgt tgaaagtcat ttcagcgatt tggtctttaa ctcccggttt
      661 acggggctcg taacagtaac tgagctgaaa tacccggtga cagtcacggc cgcggaatcg
      721 ctcatgtctt tttgggttct gcccgtgacg gtaaacctga acggactggc aacgggggca
      781 atgaaggtta actgtgacca tgaaacaacc ttaaaagcga gtattatatc cttaattcaa
      841 ctaattggag gcatcaccgt ttaccggagc gacagcttgc ggaaaggtcg tgcaactaca
      901 aagcaacaaa cagactacgg cgggaattat ttttttcatc atccattacc acgaaaacag
      961 cggccactct ttggcgactg tggtgagttg tggcgcgtcg aaaacggctt gtggtgaaac
     1021 taccgtttcc tcaagcggaa tatttttgcc gtagcgttct ttgagcttag cctggaccag
     1081 cgggttgctg agatcgaact ctttcaactt ctgctgctcg cccacgcgca ttcccagcgc
     1141 gttctggtaa accttccaca ccacttccga acagtactgg cgatcgtcgc tccatgagaa
     1201 gctgaaatca tacggtttac cgagataacg ttttgccgtt tgcgccagtt tttgctgttg
     1261 ttcaacactc agtccgcctt caacgcggcg aacaacgtat ttgccctttt caccatgggc
     1321 gatccactgc ttgagcgggg tgtatttcac cgggccgacc gcttcaaaaa cgtagggctt
     1381 tttgttgcgt atcaccagca taccggtgtg gctataatcg gaatgggtcg ccagttggat
     1441 cgctttactt tgcgatgagc gagagatctg aaagatgatg tcgccggttt gtggctgcca
     1501 ggcaaaggca ggtacagaaa ccagcaggct gaggatcagc agcctgtttt tcatagttaa
     1561 acgtccatgt ataaaaagcg gtgggtcgca gacaacgtgc tcgttgttta tgccggatgc
     1621 ggcgtgaacg ccttatccgg cctacaagtt cgtgcaaatt caataaattg caatatgacg
     1681 taggcctgat aagcgtagcg catcaggcga ttccactccg cgccgctctt ttttgcttta
     1741 gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg ccgcttccgg
     1801 gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt gcccggtaaa
     1861 ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca ggttgttcac
     1921 ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa tggaaaccgt
     1981 aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga tatattcgcc
     2041 gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac cagaccacac
     2101 aatttatgtg ggtactgacc gtaaacccgc atagtttacc gtacaggcgt taccgtgaca
     2161 tcgtgtaatg cacctgtcgg cgtgataatg catataattt taacggctat ttgggatttg
     2221 ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat taacacaatg
     2281 tttactctgg tgcctgacat ttcaccgaca aagcccagga aacttcatca catgacgcgt
     2341 aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta tggttgcgtt
     2401 gtccctccca tccatctttc cagcacctat aactttaccg gatttaatga accgcgcgcg
     2461 catgattact cgcgtcgcgg caacccaacg cgcgatgtgg ttcagcgtgc gctggcagaa
     2521 ctggaaggcg gtgctggcgc agtacttact aataccggca tgtccgcgat tcacctggta
     2581 acgaccgtct ttttgaaacc tggcgatctg ctggttgcgc cgcacgactg ctacggcggt
     2641 agctatcgcc tgttcgacag tctggcgaaa cgcggttgct atcgcgtgtt gtttgttgat
     2701 caaggcgatg aacaggcatt acgggcagcg ctggcagaaa aacccaaact ggtactggta
     2761 gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg ccatctggca
     2821 agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc attacaaaat
     2881 ccgctggcat taggtgccga tctggtgttg cattcatgca cgaaatatct gaacggtcac
     2941 tctgacgtgg tggctggcgt ggtgattgct aaagacccgg acgttgtcac tgaacttgcc
     3001 tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct gctgctacgc
     3061 gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc gcaacgcgca ggcgattgtg
     3121 aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct gccggaaaat
     3181 caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt gagttttgaa
     3241 ctggatggcg atgagcagac gctgcgtcgt ttcctgagcg ggctgtcgtt gtttacgctg
     3301 gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat gacacatgca
     3361 ggcatggcac cagaagcgcg tgctgccgtc gggatctccg agacgctgct gcgtatctcc
     3421 accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt ccgggctgca
     3481 aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt cagctgcata
     3541 aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg ggcattatgg
     3601 cggagtactc tcagcctgac gatatgatgg tggtttccgc cgccggtagc accactaacc
     3661 agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat caggttcaac
     3721 aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgct gaagaagccg
     3781 atagcctcat tagcgctttt gtcagcgatc tggaacgcct ggcggcgctg ctcgacagcg
     3841 gtattaacga cgcagtgtat gcggaagtgg tgggccacgg ggaagtatgg tcggcacgcc
     3901 tgatgtctgc ggtacttaat caacaagggc tgccagcggc ctggcttgat gcccgcgagt
     3961 ttttacgcgc tgaacgcgcc gcacaaccgc aggttgatga agggctttct tacccgttgc
     4021 tgcaacagct actggtgcaa catccgggca aacgtctggt ggtgaccggg tttatcagcc
     4081 gtaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat tccgcgacac
     4141 aaatcggtgc gctggcgggt gtttctcgtg taaccatctg gagcgacgtc gccggggtat
     4201 acagtgccga cccgcgtaaa gtgaaagatg cctgtctgct gccgttgctg cgtctggatg
     4261 aagccagcga actggctcgt ctggcggctc ccgttcttca cgcccgtact ttacagccgg
     4321 tttctggcag cgaaatcgac ctgcaactgc gctgtagcta cacgccggat caaggttcca
     4381 cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc agccacgatg
     4441 atgtctgttt gattgagttt caggtgcccg ccagtcagga tttcaaactg gcgcataaag
     4501 agatcgacca aatcctgaaa cgcgcgcagg tacgcccgct ggcggttggc gtacataacg
     4561 atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg ctgaaaatcc
     4621 tcgacgaagc gggattacct ggcgaactgc gcctgcgtca ggggctggcg ctggtggcga
     4681 tggtcggtgc aggcgtcacc cgtaacccgc tgcattgcca ccgcttctgg cagcaactga
     4741 aaggccagcc ggtcgaattt acctggcagt ccgatgacgg catcagcctg gtggcagtac
     4801 tgcgcaccgg cccgaccgaa agcctgattc aggggctgca tcagtccgtc ttccgcgcag
     4861 aaaaacgcat cggcctggta ttgttcggta agggcaatat cggttcccgt tggctggaac
     4921 tgttcgcccg tgagcagagc acgctttcgg cacgtaccga ctttgaattt gtgctggcag
     4981 gcgtggtgga cagccgccgc agtctgttga gctatgacgg gctggacgcc agccgcgcgt
     5041 tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc ctgtggatgc
     5101 gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag cagcttgctg
     5161 atcagtatct tgatttcgcc agccacggtt tccacgtcat cagcgccaac aaactggcgg
     5221 gagccagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa accgggcgtc
     5281 actggctgta caatgccacc gtcggtgcgg gcttaccgat caaccacacc gtgcgcgatc
     5341 tgatcgacag cggcgatact attttgtcga tcagcgggat cttctccggc acgctctcct
     5401 ggttgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat caggcgtggc
     5461 agcagggctt aaccgaacct gacccgcgtg acgatctctc tggcaaagac gtgatgcgca
     5521 agctggtgat tctggcgcgt gaagcaggtt acaacatcga accggatcag gtacgcgtgg
     5581 agtcgctggt gcctgcccat tgcgaaggcg gcagcatcga ccatttcttt gaaaatggcg
     5641 atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg gggctggtgc
     5701 tgcgctacgt ggcgcgtttc gatgccaatg gcaaagcgcg tgtgggcgtg gaagcggtgc
     5761 gtgaagacca tccgctggca tcactgctgc cgtgcgataa cgtctttgcc atcgaaagcc
     5821 gctggtatcg cgataatccg ctggtgatcc gtgggcctgg cgctgggcgc gacgtcaccg
     5881 ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt cagaaattta
     5941 ataatgcccg gtactcatgt tttcgggttt atggtttcta atgaaatata ttgaattatc
     6001 ataggattag gccggattaa gcgtttacga cgaatccggc aagaagcaat aagtacatgg
     6061 ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt cttaccatcc
     6121 tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt tcggttgacg
     6181 cccttcggct tttccttcat ctttacatct ggacgtctaa acggatagat gtgcacaaca
     6241 caacatataa ctacaagcga ttgatgaggt aaggtatgag cttttttcac gccagccagc
     6301 gggatgccct gaatcagagc ctggcagaag tccaggggca gattaacgtt tcgttcgagt
     6361 ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc gatcgcctta
     6421 gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc gagcgcgacc
     6481 gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg gcaccgcatc
     6541 ttacttgcat tgatgcgacg cccgacgagt tgcgcaccat tgcacgcgac tactggaata
     6601 acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt ggtaagccag
     6661 aaatgtatgc ttctgacctg gtgacgctat taaaagaagt ggcagatttc gatatctccg
     6721 tggcggcgta tccggaagtt cacccggaag caaaaagcgc tcaggcggat ttgcttaatc
     6781 tgaaacgcaa agtcgatgcc ggagccaacc gcgcgattac tcagttcttc ttcgatgtcg
     6841 aaagctacct gcgttttcgt gaccgctgtg tatcggcggg cattgatgtg gaaattattc
     6901 cgggaatttt gccggtatct aactttaaac aggcgaagaa atttgccgat atgaccaacg
     6961 tgcgtattcc ggcgtggatg gcgcaaatgt tcgacggcct ggatgatgat gccgaaaccc
     7021 gcaaactggt gggcgcgaat attgccatgg atatggtgaa gattttaagc cgtgaaggag
     7081 tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg atttgccata
     7141 cgctgggtgt tcgacctggt ttataaatag tgtggctttt gtgaaaatca cacagtgatc
     7201 acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgggaaa ataaggttgt
     7261 cagccttgtt ttctccctca ttacttgaag gatatgaagc taaaaccctt ttttataaag
     7321 catttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt gacctacatc
     7381 tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat tcaattataa
     7441 cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac attgatgagc
     7501 acgtcagacg atatccataa cacgacagcc actggcaaat gcccgttcca tcagggcggt
     7561 cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa tcaactccgt
     7621 gttgacctgt taaaccaaca ttctaatcgt tctaacccgc tgggtgagga ctttgactac
     7681 cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa agccctgctg
     7741 acagaatctc agccgtggtg gccagccgac tggggcagct acgccggtct gtttattcgt
     7801 atggcctggc acggcgcagg tacttaccgt tcaatcgatg gacgcggcgg cgcgggtcgt
     7861 ggtcagcaac gttttgcacc gctgaactcc tggccggata acgtaagcct cgataaagcg
     7921 cgtcgcctgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg ggccgacctg
     7981 tttatcctcg caggtaacgt ggcgctggaa aactccggct tccgtacctt cggttttggt
     8041 gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga tgaaaaagcc
     8101 tggctgactc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc aaccgagatg
     8161 ggcctgattt acgttaaccc ggaaggcccg gatcacagcg gcgaaccgct ttctgcggca
     8221 gcagctatcc gcgcgacctt cggcaacatg ggcatgaacg acgaagaaac cgtggcgctg
     8281 attgcgggtg gtcatacgct gggtaaaacc cacggtgccg gtccgacatc aaacgtaggt
     8341 cctgatccag aagctgcacc gattgaagaa caaggtttag gctgggcgag cacttacggc
     8401 agcggcgttg gcgcagatgc cattacctct ggtctggaag ttgtctggac ccagacgccg
     8461 acccagtgga gcaactattt cttcgaaaac ctgttcaaat atgagtgggt acagacccgc
     8521 agtccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat cccggatcca
     8581 ttcgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac gctgcgtttt
     8641 gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc gttcaacgaa
     8701 gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa atctcgctac
     8761 atcgggccgg aagtgccgaa agaagatctg atctggcaag atccgctgcc gcagccgatc
     8821 tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga ttctggtctg
     8881 tctgttagtg agcgggtatc ggtggcctgg gcatctgctt ctaccttccg tggtggcgac
     8941 aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga ctgggatgtg
     9001 aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa tccagaaaga gtctggtaaa
     9061 gcctcgctgg cggatatcat cgtgctggct ggtgtggttg gtgttgagaa agccgcaagc
     9121 gccgcaggtt tgagcattca tgtaccgttt gcgccgggcc gcgttgatgc gcgtcaggat
     9181 cagactgaca ttgagatgtt cgagctgctg gagccaattg ctgacggttt ccgtaactat
     9241 cgcgctcgtc tggatgtttc caccaccgag tcactgctga tcgacaaagc ccagcaactg
     9301 accctgaccg cgccggaaat gaccgcgctg gtgggcggga tgcgcgtact gggtgccaac
     9361 ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag caatgacttc
     9421 ttcgtgaact tgctggatat gcgttacgag tggaaagcga ccgacgaatc gaaagagctg
     9481 tttgaaggcc gtgaccgcga aaccggcgaa gtgaaataca ctgccagccg tgcggatctg
     9541 gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag tttacgccag cagcgatgct
     9601 cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa cctcgaccgt
     9661 ttcgacctgc tgtaatctga ccttcttcag cgactgcctt tcaggcagtc gctgaagttt
     9721 ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat gtctgccgca
     9781 ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat ctggagttat
     9841 agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt taccgcctta
     9901 cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg tcgcggaatg
     9961 cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg cgggatggtt
    10021 ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat cctaagctat
    10081 accatgccgt tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg cctgcgacgt
    10141 gggcaatatt tcgcgatact gattgccgct ttcggcttat ttttggtgtt gcagccgtgg
    10201 caactcgatt tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg cgtcagttgg
    10261 ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt ggatttattg
    10321 tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt cgctttactg
    10381 gtgccgcaac gtgaaattga ctggcagccc accgtgttct gggcgctggc ctacagtgcg
    10441 attctggcga cggcactggc gtggagctta tggttgtttg tattgaaaaa cttgcctgcc
    10501 agtattgcca gcttaagcac actggccgtt cccgtttgcg gcgtactctt ttcctggtgg
    10561 ctgctcggcg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat tgtgatggca
    10621 ctggcgctgg tgagccgtaa gaaaaaagaa gccgttagtg taaaaaggat ctgaattttt
    10681 tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac catgcgccag
    10741 ctaaacagca catcttcttc ctgcgcacct gcgccaatgt tatgtatcac cagcggcgta
    10801 ccgtcgcggg cgaagccatc tgaaaccacc ccaatatgtg ccagcccgtt atctaatcgc
    10861 caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg gcgtgtttta
    10921 tcgtggtggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat gttgctgtcc
    10981 gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat atcttcgtgc
    11041 accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac atcggaacat
    11101 accccgcgtt tttgcgggac atcaccgcca ggataggtaa gctgcatata cgccggatcg
    11161 taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag gttggtattg
    11221 gcctggattt gtaccacggt tggtggaacg gcgggagatt ttaaggagtg gctggtaaat
    11281 gccgttagca ggctgagcag cgccagtgaa gctttcatct ggttcgtccg tgaaaataag
    11341 agtcacagat tcgaccttcc gggataacga ttgttctgta gagaaaatgt ccagaagcgg
    11401 ataaaaaact cctgaacaac gtgggagttt cagattactg gcacagagtg ccggatgcgg
    11461 cgtgaacgcc ttatctgtcc taaaaaacaa gaaaattcaa tatattgcag agattgcgta
    11521 ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag
    11581 tttggagtag gttattccca ctcttgcagg aaacgctgac cgtactggtc ggctaccagc
    11641 agagcggcgt aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacat
    11701 tctgcacatg ccgcttcagc cacaattcgc attttcgccg ggacatcttc tttaatatcc
    11761 agttgagcga gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt
    11821 tcctcaaccg gtgcgttttc cagaaccagc tgcgtcagcg taccaaatgc cactttttca
    11881 ccgtgataat agtgatgcgc gtccgggata gcggtcaggc cgttatgcac tgcgtgcgcc
    11941 gcagccagac caccactttc aaaaccaaca ccgctcaaat aggtgttcgc ttcaatcacg
    12001 cgctccagcg ccggagtcac tacatgctgt tcggcagcaa gcatcgcttt ttcgccttct
    12061 tccagcaggg tgttgtagca cagttcagcc agtgccagcg cagcctgggt gcacttgccg
    12121 accgccatgg tggtcgcgcc gctacgagag caggcacgcg cttcaaacca ggttgccagc
    12181 gcatcgccga tacccgccgc taacagacgt gcaggtgcgc cagcgacgat tttggtgtcg
    12241 acaatgacca tattcgggtt atttggcaac agcagatagc ggtcaaactc accctcatcg
    12301 gtgtagataa cagacaatgc gctgcacggt gcatcggtag aggcgatagt cggtgcgatc
    12361 gctaccggaa cacccatgaa atgtgccagt gctttggctg tatcgagggt ttttccgcca
    12421 ccgataccga gaattgcgcc acactgcgca gtctccgcga tgccacgcag acggtcgatc
    12481 tcattttgcg aacatccacc gccaaacggc gcaatttcta ctaccagtcc agcatcttta
    12541 aagcttttct cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc caccactaac
    12601 cagcgttctg ccagcggctt caggtattcg cccagacgat taatcacatc agcgccctgg
    12661 atgtatttac ccggtgattg aataatgcgg tccataattg ctcctttaga gatgagtagt
    12721 gccaaatgcg gcattccagt cgtgttcgaa cttctctata gctgactcta ccgcaggggt
    12781 gttgagcatt tgttgcgcta catctaaggg cagggtgatg gattcacatc ctgccagtaa
    12841 acagtccagc gcctgacgcg gcgttttaaa gctggctgcc agcaccatgc tttctggcgc
    12901 gtgcatttct aacagcgctt gcagctcctg aaccgtacga atgccgtctc cgccctgggc
    12961 atctacgcgg ttaacatacg gagcaacgta ttttgcccct gccagcgcgg ccagtaaccc
    13021 ttgtgcagca ctgtacactg cggtgccaag cgtagtaata ccctcttttt tcagtatttt
    13081 aattgctgcc agaccttcgg aagtcaccgg gattttcacc acaatacccg gaatagcgtc
    13141 gcgcaggcgc ttcgcttctt ccaccatccc ctgcgcgtcg cggctcatgg tctgagcaaa
    13201 cagaatgccc tcatcaccaa tcgctttttg cagacgcggc agcacttccc atatggactc
    13261 cttgctggca gcgataatgc tcgggttagt tgtcaccccg gcaatgggga atatgcgtgc
    13321 cagacgttcg acttctgcga cgttagcggt gtccagatac agttccatga tgtttccttt
    13381 tacagttcca gttcatgttg cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc
    13441 agcgcgttac ggaattcttc gtgcatgatg cggcgagcga ggcgcgagaa aatgcgcatg
    13501 tgctgatcgc ccgcagcgtg tttgttcagg gttaacatga tgatgaattg cgcttcatca
    13561 tcaccccagc gcaccggcgc ttgcagacgc gcaacgctga tggtggattg ctcaatgtgt
    13621 tctgatttgc tgtgtggaat ggcaaaacta aagcccagac cggtagagaa aacggcctcg
    13681 cgcgcccaca agtcggcttc cagtttacgc ggatagcggc agcgccctgc cagcagcagg
    13741 ttatcggtca tgcctttgag cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg
    13801 cactcggcgg tgaccagcgg tgcgtcttgt tgggtcatgc ggaattgcgc cagcaggtgc
    13861 tccacttcca gcgaagtacg gcaggccatt gcctggttga gcaacttgcg gcactcacgg
    13921 ctatcaagtt gcgccatccg cgctttcgcc gccggaattg atggtgcgct catgctgagt
    13981 tcatccagcc ctaagccgac cagcaacggc agcacggaac ctttcgctcc cagctcaccg
    14041 cacagaccaa tccatttacc ctggcgatgc accgcctgca cggcgtaatc gagcgcccgc
    14101 aagaatgccg gattcaggct gttgtagtga cgagtaacct tagcgttatc gcgatccact
    14161 gccagcagat attgcgtcag gtcattacta ccaatactaa agaaatcaat ctcttcgcag
    14221 cattgatcga tgatgaacat caccgacggc acttccagca taatgccgag ctggatcttc
    14281 tcatcaaacg gaatgtgttc gttacgtagt tgctgtttgg cttctgccag tttttctttc
    14341 acccataaga tctcttccat tgaggagatc atcgggatca tgattttcag gctgccgtga
    14401 gcggaggcgc gcaggatcga gcgtagttgt gtggtaaata acgacgcgta ttcttcataa
    14461 atacgcacgg cgcgataacc gaggaacggg tttgcctctg cgggaatgtt tagataatca
    14521 acgggtttgt cgccgccaat gtccatagtg cgcacaataa tgctgcgtcc gttggcagac
    14581 tccagcgcct gacaaaaaat gttgtacagc tcgctttcgc ccggtgcact ggtgcgatcc
    14641 atatagagca tttcagtgcg gaacaaacca acgccttccg caccattgcc gaatgcggcc
    14701 tgcgcttcca cggagtgagc gatgttagcg gcaatttcaa tgcggatacc gtcagcggta
    14761 cgggcttgtt gggtcagcca gacacgctgt tgctcacgca gggcgtcctg tacgcgggct
    14821 tcttgctgat aataacgagc tacggcttct cctggctcaa ccacaatcgc cccggcgttg
    14881 ccgtcgatat aaatcgtttg atgctgccac ggagtgaggg catcaatatc cacaccaacc
    14941 agcgtcggaa tgttgaacga acgggcaagg atcaccgtat gtgaggtggt gccgccactt
    15001 ttcagcaaca gacctttgag gtgattttta tccagttcga ggaactggct gggggtcagt
    15061 tcatcagcca tacaaatggc ggactgcgtc agtttgcccg gtgccgggaa gcgttgctca
    15121 ccgtagattt gctggagtaa ctggaagcag acgtcgcgta cgtccagggc acgttcttgc
    15181 aggtagctgc tgctggaacg ggcgaactct tcgcaaaagt gattcgcact ggcgacaatc
    15241 gcttcggcgc agcttaatcc ctcgctgacg cctgccagta agtgctgacg taaagatgta
    15301 tcgccagcca gggatcggtg agcttccagt atcgcgctgg tagcaccgtc gctatccagc
    15361 agacgaaact caatgttttt cagtaccagc gtcaggccgt tttccagtgt ggattgctcg
    15421 gcgtcaacgt ctttggctgc gggaagatta ctcagcgcat tgagatctaa agaagagatc
    15481 ggcgtcagaa tgccgcctgc actaccgctg cacacggtgc gggcgcggat aatttgcgga
    15541 ttcagattgg tcagtgaaac cggcagtggt tccagttcgt cagatttaac ttccgccagc
    15601 ggcgcatcgc agtgggggaa ttcatcgcgc agccattggc ttaaacgctg gtgcgcttcc
    15661 tgttcgtcgg ccccggaaat cagtaactgg cagttatcgc ccgccagcgt atcggtacca
    15721 atcagcgcaa gggcgctttt ggcgttgccc ttgcggtcag tgtgcaggtt atgccactca
    15781 atttgtgatg aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg cgcatgtacg
    15841 ccgttaggta gctcacaaat aaattccaca atcagggcca ttgcctctct cccggtacgg
    15901 ttttctgttt acaggataag agcgcacggc aacggcctgc catgtgacaa atctgccaaa
    15961 agctggacaa atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc
    16021 ggcatgcttc cggtttatcg caagttatga ggcggatcgc atttttgtac tgatattaca
    16081 aaagtccagt aaatggcctt tttatccact gtttgctccg cctgcgattg cctattgttt
    16141 cctccatcaa aaatatggac atgggctttc gcaagcccgg gagcagctta tgaatgagtt
    16201 ggtgcagatc ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac acatgattcc
    16261 cttcgtggta tcgggcggta ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc
    16321 agtgccggat gccgtagccg atccaaatct gaaaaaactg tttgatatcg gcgttgcggg
    16381 cttgacgctg atggtgcctt tcctcgccgc ttacattggt tattccattg cagaacgttc
    16441 tgcgctggct ccatgcgcta tcggtacctg ggttggtaac agctttggtg cgggcttctt
    16501 tggtgcactg atcgccggga ttatcggcgg catcgtggtg cattacctga agaaaattcc
    16561 ggtgcataaa gttctgcgct cggtgatgcc tatcttcatt attcctatcg tcggcacact
    16621 gattaccgca ggcatcatga tgtggggctt gggcgagcct gtaggggcgt tgaccaacag
    16681 cctgactcag tggcttcagg ggatgcagca gggcagcatt gttatgctgg cggtgatcat
    16741 gggtctgatg ctggcgttcg atatgggcgg tccggttaac aaagtggcct atgccttcat
    16801 gctgatttgc gttgctcagg gtgtttatac cgtggtggcc atcgctgccg tgggtatttg
    16861 tatcccgccg ctggggatgg ggctggcgac gctgattggt cgtaaaaatt tctccgcaga
    16921 agagcgcgaa accggcaaag cggcactggt gatgggctgt gttggcgtta ctgaaggggc
    16981 gattcctttc gccgctgccg atccgctgcg tgtcattcct tccatcatgg tcggttcagt
    17041 ttgtggtgca gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct ggggtggtct
    17101 gattgtgctg ccggtggttg aaggcaagct gggttatatc gcagcagtgg ctgtcggagc
    17161 agtggtgacg gctgtttgtg tgaacgtgct gaaaagtctg gcgcgtaaaa atgggtcttc
    17221 gactgatgaa aaagaagacg acctggattt ggattttgaa attaattaat tgaggaacga
    17281 gccatgacga aaattattgc agtaaccgca tgtccttcag gtgttgccca tacctatatg
    17341 gcagcagagg cgctggaaag tgccgcgaaa gcgaaaggct gggaagtgaa agtagaaacc
    17401 taggggtcaa ttggtctgga aaatgaactg actgcggaag atgtggcgag cgctgatatg
    17461 gtgattctga ccaaagatat cggcatcaag tttgaagagc gttttgctgg caagaccatc
    17521 gtgcgcgtca acatcagcga tgcagtaaaa cgtgctgacg ccattatgag caaaattgaa
    17581 gctcacctgg cgcaaaccgc ttaagttctt ctcccgctcg caagggcggg ttcgctttcc
    17641 cacaggagtt cctcatgacg aatcgtatct ctcgcctcaa aactgcactg tttgccaata
    17701 cccgtgaaat ctcgctggag cgggcgctgc tttataccgc cagccatcgg caaaccgaag
    17761 gcgaaccggt gatattgcgc cgggcgaaag caacagcgta tatccttgaa catgttgaaa
    17821 tttcgattcg tgatgaagaa ctgattgccg gtaaccgcac cgtaaaaccg cgcgccggga
    17881 ttatgtcgcc ggaaatggac ccttactggc tgctgaaaga gctggatcaa ttcccgacgc
    17941 gtccgcagga ccgctttgct atcagcgaag aagataaacg tatctaccgt gaagagttgt
    18001 tcccgtactg ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg acagatgaag
    18061 taaaagccgc gaccaacacg cagattttca gcatcaacca gacggataaa ggccaggggc
    18121 acattattat tgattaccca cgcctgttga atcacggtct gggtgagctg gtggcacaga
    18181 tgcagcaaca ttgtcagcaa cagccggaga atcactttta tcaggccgcg ttgttactgc
    18241 tggaagcctc gcagaaacac attttgcgtt acgccgaact ggcggaaacg atggcggcaa
    18301 actgcacaga tgcacagcgt cgcgaagaac tgctgactat tgcggagatc tctcgccata
    18361 acgccgaaca taagccgcag acgttctggc aggcgtgcca gttattctgg tacatgaaca
    18421 tcattctgca atacgaatcc aacgccagtt cgctgtcttt agggcgcttc gaccagtata
    18481 tgttgccgtt ctatcaggca tctttaaccc agggcgaaga tccggcgttc ctgaaagaac
    18541 tgctcgaatc tttatgggtg aaatgcaacg acatcgtgct gttgcgctcc accagcagcg
    18601 cgcgttattt cgcaggtttc ccgaccggct ataccgcact gctcggcggg ttaaccgaga
    18661 acggacgtag cgcggtgaac gtgctttcgt tcctttgcct tgacgcctat caaagcgtgc
    18721 aattaccgca accgaacctc ggcgtgcgca ctaacgcctt gatcgacacg ccgttcctga
    18781 tgaaaaccgc cgaaaccatt cgcctcggca ccggtattcc gcaaatcttt aacgatgaag
    18841 tggtggtacc agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc gactattccg
    18901 tagtgggctg tgtggaatta tctattcccg gcagaaccta cggcttgcat gacatcgcga
    18961 tgtttaatct gctgaaagtg atggaaatct gcctgcatga aaatgaaggc aatgctgcgc
    19021 tgacttatga aggtttactg gagcagatcc gcgccaagat cagccactac atcaccctga
    19081 tggttgaggg cagcaatatt tgtgatatcg gccatcgcga ctgggcacct gtaccgctgc
    19141 tctcgtcttt tatcagcgat tgtctggaaa aaggtcgcga tattaccgat ggcggcgcgc
    19201 gttataactt ctccggcgta caggggatcg gtatcgccaa cctgagcgat tctctccatg
    19261 cgttgaaagg gatggttttt gagcaacagc gtttaagttt tgacgaattg ctgtcggtat
    19321 taaaagccaa ctttgcaacg ccagaaggcg aaaaagtccg tgctcgctta attaaccgct
    19381 tcgagaaata cggtaacgat atcgacgagg tggataacat cagcgccgaa ctgttacgcc
    19441 actactgcaa agaagtggaa aaataccaga acccgcgcgc ggttacttca cgccgggatc
    19501 gtataccgtt tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg
    19561 ttttgccggg gaacaactgg cagacggcgg cttgtcacct atgctgggcc aggacgcaca
    19621 agggccaaca gcggtactga agtcagtcag taagctcgat aatacgctgc tgtctaacgg
    19681 tacgttgctg aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa
    19741 actggccgac ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt
    19801 ggtgaacgcc gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt
    19861 ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga
    19921 catcatccgc cggacagcgc atcagctgta acgttgtgga aacgcgccgc gatgatgtgg
    19981 cgcgcatttt caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg
    20041 tcttttttaa aggctgtccg catctttgcc cgtggtgtgc taatccggag tcgatctccg
    20101 gcaaaatcca gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg
    20161 atgcggatga atgcccctcc ggggcgtttg aacggattgg tcgcgatatc agccttgacg
    20221 ctctggaacg ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca
    20281 cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt ttacagcgac
    20341 tgcggctgtg gggtgtgtca tgcgccattg aaactgccgg agacgcgcca gcaagcaagc
    20401 tgttaccgct ggcgaaattg tgcgatgaag tgctgttcga tttaaaaatt atggacgcga
    20461 ctcaggcgcg ggatgtggtg aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc
    20521 tggtgagtga gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt ttcacgctca
    20581 gcagggagaa tatgcagcag gcgctggatg tactgatccc gctgaaaatc aggcagatcc
    20641 atctgttacc gtttcatcag tacggcgaac cgaaataccg cctgctgggg aaaacatggt
    20701 cgatgaaaga ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag
    20761 aacgggccgg atttcaggtt accgtgggag gttaaaatgg catacctggt ggcagtaacc
    20821 gcctgcgtaa gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc
    20881 cagttagaga agtggggcgt cagcattgaa actcagggcg cgctgggaac ggagaatcgt
    20941 ttagcggacg aggatatccg tcgggcggat gttgctctgt tgattacgga tatcgagctt
    21001 gccggtgccg agcgatttga acattgccgc tatgtgcaat gcagcatcta cgcattcctg
    21061 cgtgagccgc agcgggtaat gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc
    21121 catcttattc tggagtagtc ggtttttctg tcagttggct gtggtactgc cggcgatatt
    21181 ccgacggcga gcgttcggtg tttttacgaa acagacggca gaagtagttg ctgtcgacaa
    21241 agccgcaggc gtgcgccact tcttttacct tcaggtcata gcctttcaat aacgtcttag
    21301 cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa
    21361 acaggtgcga gagatagttt ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa
    21421 gcgcggaggc atagcgttcg tcgatataat cgcgaatagc ttcaaatagt gcctggctgc
    21481 gtgaggcggt ctggatttgg ctgccgagca aatcgcggca gtggctaagc aggctggcta
    21541 cgataagcct tgccgtttgc tgctcctgcg gctgcatttg catttcattg agcgtttgta
    21601 gcagaaaaga acctatacgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat
    21661 attgtttgcc atcccattgc acgacgctga agccgagttg ctgtttgcca aacagcacgc
    21721 taaaggtggt agcgggtgtt ttccattgtg ggaaattcca gccaccagcg gcaacgtaca
    21781 gcacatcgcc gggaactaac gttgctccag cgccggtatc aacaaactca ccttccagca
    21841 caatttccag ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttactgc
    21901 tggcaaagta aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc
    21961 tgacgtcgtg atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt
    22021 attaaaaaag gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca
    22081 gttgtattgc cggatgtggc gtaaacgcct tatccagcct accgccgcga tctgtaggcc
    22141 ggataagacg cgtcaagcgt cgcatccggc aaatagcgcc tggctgataa ttactgatta
    22201 cctacctggt cgccatacgg cagagtgtcg taatcgatca gcgcgttttt cttgtacggg
    22261 ttaccaatcc agcgggtagt ttctttgaac tgcggattca ccactgaacg ggttggatcg
    22321 taaccgtcgt aagataagcc cgccaaatct gaccaggtgt ggatcagttc cgccaggctg
    22381 tatttacgat cgacatcctg tgagaaatca cggggatgag tcgcttgcca tttttccgac
    22441 gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg ggttgtcttc gttacgcccc
    22501 tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa gtaaaccagg
    22561 aatccgttcg ggtctgccgc tttaaagtct ttaatcaggc tggcaaccac atgatcgtta
    22621 tacaggttag cgttgtcata atcgttatat gattccagct cttctgcgct taatcctggc
    22681 ggaacatgat cggtattgcc atcaaacttg ccctgatttt ccgggtagcg gtatttgtat
    22741 ttgatatgcg tacccagcag atgaacgatg atcagtttct tcggcgcagg gtcattcagc
    22801 acatcctgga acggcttcag cacgttggtg tcgtattcac gcgcactctg cgtacgttgc
    22861 tggttcatgt agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat ggtattgcgg
    22921 gcggtcatcg tctgctggtt ggtgatccag aaggttttat aacccgcctg tttcatcatg
    22981 ttcatcagcg acggctgcgt cagatacaga tccgggttct tttcattggc aaaggtcagc
    23041 gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt aaacacggtc
    23101 agattcggat cggttttatg cagcgcatcc agctccggcg tggtttcacg cggataaccg
    23161 tacagactca tgcgtccgcg ctgggtcgac tcgccaatca ccagcactaa agtgcgcggt
    23221 tcgttacccg attcatcttt gaaattagcc agtggcggca aggcattatt ttcattcagt
    23281 aactttgtta gcgagtttag ttgctgacga tactgataat agccggtcag gaattgccac
    23341 ggtgcggcag gctccatacg cgaggccagg ttatccaacg ttttctcaaa cggcttgttt
    23401 ttgataaacg tattcatggc gatcggatgc agaatcaagc cataaagcag ggcaaaagag
    23461 acaacataac gccacggctt tggaatatag accgggcgca ggcgtgtcca cagcagaact
    23521 gccaccgccg tataggccag cgcgataagc acaattttca ggctgaaata ctggcttaaa
    23581 tactcgctgg cttcgttggt gttggtttcg aacatcacaa acagaacgct ctgcgagaac
    23641 tcctgcccgt agatgacgta gtagcatagc gccgccagag aggccgccca tagcaccacg
    23701 ccgattactg cggcaataat tttaatccgc ttcggaaaga ggaataccgg gatcaaccac
    23761 agcgagctga ataacagcga gtcgcgaatg ccgttggtgc cactataacc actgatgtaa
    23821 ataatggcct gtagcagagt agagaaaaac caaaagtaga gcagtgccca acccagggct
    23881 ttccagctaa aaaggggttt agcctggact tctgtggaat gcatagtaag aacctgtctt
    23941 gaaaaaaata tcgccgaatg taacgacaat tccttaagga tatctgaagg tatattcaga
    24001 atttgaataa aatgcagaca gaaatatatt gaaaacgagg gtgttagaac agaagtattt
    24061 cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga ggaagattag ccggtattac
    24121 gcatacctgc cgcaatcccg gcaatagtga ccattaacgc ttgttcgacg cgaggatccg
    24181 gttcctggcc ttctttttct gcctggcggg agcggtgcag caactcggcc tgcaatacgt
    24241 tcagcgggtc ggtgtaaata ttccgtagct gaatagactc tgcaatccac ggcagatcgg
    24301 ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct tcttgcaggt
    24361 tgcgtaactc tttacctaac ggccacagtg ctttgtctac cagacgttga tcatagtatt
    24421 ccgccaacca caggtctgct ttggcgaaga ccatctccag catgccgaga cgcgtcgaga
    24481 agaatggcca atcgcggcac atggcttcca gctcgctctg tttgccatct tcgaccactt
    24541 tttgcagcgc cgtacctgca cccagccagg cggggagcat caggcggttt tgcgtccagg
    24601 caaaaatcca cggtatggcg cgcagcgact cgacgccgcc ggttgggcga cgtttcgccg
    24661 gacgtgaacc caacggcagt ttgcccaatt cttgttccgg cgtagcggag cggaagtaag
    24721 gcacaaaatc tttgttttca cgtacgtagc cacggtagag atcgcaggag atgactgaca
    24781 gttcatccat aatgcgacgc cagctctctt tcggctcagg tggtggcagc aggttggctt
    24841 ccagaatcgc cccggtgtaa agcgacaggc tgctgacggt gatttctggc agaccatatt
    24901 taaagcggat catctcgccc tgttcagtca cgcgcaggcc acctttcagg cttcctggcg
    24961 gttgtgacag cagcgccgca tgggcaggtg cgccgccgcg accaatggaa ccgccgcgac
    25021 cgtggaacaa cgtcagctca atacccgctt tttcgcaggt tttgattaat gcatcctgtg
    25081 cctgatattg cgcccaggaa gctgccatca cgcccgcatc ttttgctgag tcggaatagc
    25141 caatcatcac catctgtttg ccctgaatca ggccgcgata ccagtcgata ttgagcagct
    25201 gggtcatgac atcgttggcg ttgttcaggt catcgagggt ttcaaacagc ggagcaaccg
    25261 gcatcgcaaa cccgataccc gcttctttca gcagcaggtg gacagccagt acgtcggacg
    25321 gcgttttcgc catcgagatc acgtaggcgg caatggagcc ttgcggcgct tcggcaatca
    25381 cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg cgcggcagaa
    25441 gcggacgttt ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc tctgaccagc
    25501 tttcgtagtc gccgataccg aggtagcggg tcagctcgcc cagcgcttcg gtatggcggg
    25561 tgctctcctg acggatatca atacggacca gcggtacgcc gaaacatttc acgcggcgca
    25621 gggtgtcgag cagatcgccg ttggcgataa tacccatgcc acacgcctga agtgactggt
    25681 agcaagcgta gagcggttcc cacagttctt cgttttgtgt cagcagccct tctggttttg
    25741 gcagttcttc gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc aggcgagaac
    25801 gcaggttttt catcagatag cgatacggtt ctgcggcacc ttcttcgcca accagcgcca
    25861 gcagttcagg ggtcgcttca accatcgaca gttcagaaac cagaacctga atatctttca
    25921 ggaacagatc ggtggctttc cagcgactga gtagcaggac gtggcgggtg atatcggcag
    25981 tgacgttcgg gttgccgtcg cggtcaccgc ccatccacga agtaaaacgg accggaacaa
    26041 attcgacggg cagtttgtag ccgaggttct cttccagttg ttcgttcagt tcgcgcaagt
    26101 aattcggtac gccttgccac aggctgtttt ctactacggc aaagccccat ttggcttcat
    26161 ctaccgggct tggacgcagc ttacggattt catcagtatg ccatgactgg gcgattaact
    26221 ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc gatatctttg ttatcgagct
    26281 gttttaagca ggcgttcact tccaccattt tgtggatcag tgtacgacgg gtaatttcgg
    26341 ttgggtgagc cgtgaggacc agttccagcg acagcgattc cactgctttt ttgatggtgt
    26401 cttcgctcag ttccggctgg tttttcagtt tgcgcagggt gcgggcgatc acttccgggt
    26461 tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg ttggctaggt
    26521 tcaggaactg actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac aaattttgta
    26581 aggtggtgag caactcctgg cggttagcat cattgccagc gcgtgaagat ttcgacaact
    26641 tacggatagt ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg atggtttctc
    26701 ccagcacttt gccgagcata ctgacattac tacgcaatgc ggaatattgt tcgttcatat
    26761 taccccagac accccatctt atcgtttgat agccctgtat ccttcacgtc gcattggcgc
    26821 gaatatgctc gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa gcggtgacgt
    26881 caaatgctgc gaaatcgctt cagcaaacga ataaatagca ggaatttacg ccattaaatt
    26941 cacgacgctt taaataagcg taacttatgg aaatgttaaa aaatcgccat acataacacc
    27001 aaaggtgtag gtcggataag atgcgcaagc atcgcatccg acattattgc ggcaccggag
    27061 tttggcaacg gtgccggatg cggcgcgagc gccttatccg gcctacgttt taatgccagc
    27121 aaaaatggtg aattacctgg ataatcaact cacgggtggg tttaataaac cgcgtctcca
    27181 gatattcatc aggctgatga gcttgattga tggaacccgg accgagcacc agcgtcgggc
    27241 ataacgtttg aataaacggc gcttcggtac agtaattcac cacttcggtt tttgctccga
    27301 gcaatttctc aaccacttca accagttgat gattcggtgg gcattcatag ccagggatcg
    27361 gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc ggagccaatg
    27421 catcgttgag caaaccatta agttcattga gtgtcatgcc aggcagcgga cgaatatcca
    27481 tatgcaactc acagcaagcg caaatacggt tagaagcgtc gccaccgtga atatgcccga
    27541 ggttgagcgt agggtatggc acggtaaatg cttcgtagtg ataacgttct ttcaggttat
    27601 cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag ttcgatagcg ttaactccgc
    27661 gtgctggatc gctggagtgc cccgactggc cctgaatacg gatggcgtta gagatatgac
    27721 ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg caatccgggc
    27781 gcagggcggt agtttcggca aaataacgcg ctccggccat actggtttct tcatcagcag
    27841 tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt agcgtatcaa
    27901 ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc ttgccgtcat
    27961 gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc accgtatcgg
    28021 tatgccccgc cagcaacaag ccgccagccc cctgtccgat acttgccagc atattgaatt
    28081 tgttgcgagt tcctggcaca ggctgcactt ccacattgaa gcctaaatct ttaaaccagt
    28141 ccgccagcag agtgattaaa tctgcattgc tttgatcgag tgcctcttcc gtggcgctta
    28201 ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa tggcggtaat ttgtttttca
    28261 ttgttgacac acctctggtc atgatagtat caatattcat gcagtattta tgaataaaaa
    28321 tacactaacg ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt tacgtttaac
    28381 ctggcaacca gacataagaa ggtgaatagc cccgatgttg aatacgctga ttgtgggtgc
    28441 cagcggctac gctggcgcag agctagtgac ctatgtaaat cgccatccgc atatgaacat
    28501 aaccgctttg actgtttcag cgcaaagcaa tgatgcggga aagttaatct ccgatttgca
    28561 tccgcagcta aaaggcatcg ttgagctgcc gttgcagccg atgtcggata tcagcgagtt
    28621 tagcccaggg gtggacgtag tgtttctcgc caccgcccat gaagttagcc acgatttagc
    28681 gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt tccggtgcgt ttcgtgttaa
    28741 cgacgccacc ttctatgaaa aatattacgg ctttacccat caatacccgg aactgctgga
    28801 acaggcagcc tacggtctgg cggagtggtg cggtaataaa ttaaaagaag cgaatttgat
    28861 tgcggtgcca ggctgttatc cgacggcggc acagctggcg ctgaaaccgt tgattgatgc
    28921 cgatcttctt gacctcaatc agtggccggt gatcaacgcc accagcggcg tgagcggtgc
    28981 aggacgtaag gcagcgattt caaatagttt ttgtgaagtt agcctgcaac cgtatggcgt
    29041 ctttactcat cgccatcaac cagagatcgc cacacacctc ggtgctgacg ttatcttcac
    29101 cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc gcctgaaatc
    29161 gggtgtgacc caggcgcaag tcgcgcaagt gttacagcag gcgtatgccc ataaaccgct
    29221 ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc tgccattttg
    29281 cgatatcggg tttgccgttc agggcgagca tctgattatt gtggcgaccg aagacaactt
    29341 actgaaaggc gcggcggcac aagcggtaca gtgcgccaat attcgtttcg gctatgcgga
    29401 aacgcagtct cttatttaag ggtgcaatga tgaatccatt aattatcaaa ctgggcggcg
    29461 tactgctgga tagtgaagag gcgctggaac gtctgtttag cacactggtg aattatcgtg
    29521 agtcacatca gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg gatgagctga
    29581 tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct gcgggtgacg cctgctgatc
    29641 agatagacat tatcaccgga gcactggcgg gaacggcaaa taaaaccctg ttggcatggg
    29701 cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac agcgtcaaag
    29761 tgacccagct tgatgaagag ttaggtcatg ttggactggc gcagccaggt tcgcctaagc
    29821 ttatcaactc cttgctggag aacggttatc tgccggtggt cagctccatt ggcgtaacag
    29881 acgaagggca actgatgaac gtcaatgccg accaggcggc aacggcgctg gcggcaacgc
    29941 tgggcgcgga tctgattttg ctctccgacg tcagcggcat tctcgacggc aaagggcaac
    30001 gcattgccga aatgaccgcc gcgaaagcag aacaactgat tgagcagggc attattactg
    30061 acggcatgat agtgaaagtg aacgcggcgc tggatgcggc ccgcacgctg ggccgtccgg
    30121 tagatatcgc ctcctggcgt catgcggagc agcttccggc actgtttaac ggtatgccga
    30181 tgggtacgcg gattttagct taagttttgt tggccggagg cgcagctttc cggcattgaa
    30241 tttcaaaata aggaaacaga gttatggcac tttggggcgg gcgttttacc caggcagcag
    30301 atcaacggtt caaacaattc aacgactcac tgcgctttga ttaccgtctg gcggagcagg
    30361 atattgttgg ctctgtggcc tggtccaaag ccctggtcac ggtaggcgtg ttaaccgcag
    30421 aagagcaggc gcaactggaa gaggcgctga acgtgctgct ggaagatgtt cgcgccaggc
    30481 cacaacaaat ccttgaaagc gacgccgaag atatccatag ctgggtggaa ggcaaactga
    30541 tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg gcgtagtcgt aacgatcagg
    30601 tagcgactga cctgaaactg tggtgcaaag ataccgttag cgagttactg acggctaacc
    30661 gacagctgca atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg gtcatgccag
    30721 gttacactca cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc ctggcctatg
    30781 ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt ctggatgtca
    30841 gcccattagg ctgtggcgcg ctggcgggaa cggcctatga aatcgaccgt gaacagttag
    30901 caggctggct gggctttgct tcggcgaccc gtaacagtct ggacagcgtt tctgaccgtg
    30961 accatgtgtt ggaactgctg tcggctgccg ctatcggcat ggtgcatctg tcgcgttttg
    31021 ctgaagatct gattttcttt aacaccggcg aagcggggtt tgtggagctt tctgaccgcg
    31081 tgacttccgg ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg gagctgattc
    31141 gcggtaaatg cggtcgggtg cagggtgcgt taaccggcat gatgatgacg ctgaaaggtt
    31201 tgccgctggc ttacaacaaa gatatgcagg aagacaaaga aggtctgttc gacgcgctcg
    31261 atacctggct ggactgcctg catatggcgg cgctggtgct ggacggcatt caggtgaaac
    31321 gtccacgttg ccaggaagcg gctcagcagg gttacgccaa cgccaccgaa ctggcggatt
    31381 acctggtggc gaaaggcgta cctttccgcg aggcgcacca tatcgtgggt gaagcggtgg
    31441 tggaagccat tcgtcagggc aaaccgctgg aagatctgcc gctcgacgag ttgcagaaat
    31501 tcagcccggt gattggcgaa gatgtctatc cgattttgtc gctgcaatcg tgcctcgaca
    31561 agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc ccaagcgatt gcttttgcgc
    31621 aggcgcggtt ggggtaagaa catttatatg tacaaatttg agcctggctt atcgccgggc
    31681 ttttttatgg caaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt
    31741 tattcgagtt gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc
    31801 tccgtttctg tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat
    31861 tctacctatc gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct
    31921 tgagtacctg gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag attcctgcca
    31981 cgttagccag ccgacgctta gcgggcaaat tcgtaagctg gaagatgagc tgggcgtgat
    32041 gttgctggag cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga
    32101 tcaggcgcgt accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg
    32161 cgagacgatg tccggaccgc tgcatattgg tttgattccc acggttggac cgtacctgct
    32221 accgcatatt atcccgatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga
    32281 agcacagacc caccagttac tggcgcaact ggacagtggc aaactcgatt gcgtgatcct
    32341 cgcgctggtg aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt
    32401 gctggctatc tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct
    32461 ggcaggggaa aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg
    32521 tttctgtttt gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac
    32581 gctgcgcaac atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc
    32641 gccggagcgc aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg
    32701 cactattggc ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc
    32761 agaggccatc cgagcaagaa tggatggcta tttcgataaa gtgttaaaac aggcggttta
    32821 aaccgtttaa cgcagctacc cgataggctt ccgccatggt cgggtagtta aaggtggtgt
    32881 tgacgaagta ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat
    32941 gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc
    33001 gatggaacaa aattttcagc gtgcccacgt tcatgccgac gatttgtgcg cgtgccagat
    33061 gtttaaactg ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg
    33121 ttttgcccac agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca
    33181 gatgcgcggt ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg
    33241 ccgctgatgc caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg
    33301 cggtctgata catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt
    33361 tctgtaacgc cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag
    33421 ctttcagttt tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct
    33481 tctcgtactc ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag
    33541 aatctgacat ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca
    33601 cttttacatc cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat
    33661 agataagtac atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc
    33721 gtggatgggt gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa
    33781 atttttcagc ggttagtgtt tcgacgctgc cgtccgggca atccagcgcc aacgtatgct
    33841 cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg attacgttcg taaaatccct
    33901 gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag
    33961 aagagcggag cagtcgcgaa tggtcgctgt atagtgggtt ttgattgaat tctataatgc
    34021 ggctgacggc gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc
    34081 caacattttg ataacgctcg ataactgcga cgcgcacacc ttgcttaacc aggcccattg
    34141 cagcgccttc gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg
    34201 gcatggtagg gcttacctgt tcttatacat aaaagcaaca gaatggtaac attctatcgc
    34261 gggtaagcca attgatcccc gtcatttatc tggctatatc ctgagcggcc tttgctttgt
    34321 ctgtttctta cttttgccct gacgttttat tggattttta tcgacgatac tctccgttta
    34381 agcggcaggt ttccgctgta cgtaaaagaa ccggccagta aatatgtttt attgcgttac
    34441 cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt
    34501 acttttggca aggattcaga catcgtgatg ggcataagag cgcaacaaaa agaaaaaacc
    34561 cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc
    34621 ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat
    34681 ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc
    34741 caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg
    34801 gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg
    34861 gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc
    34921 attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa
    34981 gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc gttggacgtc
    35041 ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttcg
    35101 aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat
    35161 gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttag
    35221 ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt
    35281 ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa cgttatctta
    35341 atcgcactat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac
    35401 tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg
    35461 cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg
    35521 aagttgctga gtaatcggta ttatgccgga tgctggcgca tccggcatgg gttttacttc
    35581 gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc
    35641 agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccggg
    35701 ttacaggaga tgtacaaaat acacggatac gcctgcacca ttttctcggt ttcactgtcc
    35761 agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt taagtcgatc
    35821 ccttgcaggc ggttaaactc gcgcacacca ttcatcgcct gagtaaattc ttctgccgcc
    35881 atacgaataa tttgtacgtt atcaatatgg ttagctgcga tgttgtattg cgcagcagca
    35941 accgacggct tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagcgctaat
    36001 gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc tttggttacg
    36061 tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt aaagctgttt
    36121 tctacctgac ggtagatcat ctctttccct gcgaccggca gacgctcgtc gatgtaatct
    36181 tgatccagct cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc
    36241 gcgcgcagtg catcgcgcag ggcctccgct tcctgacgcc actcatcatc cagcttctta
    36301 tggtatagca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac
    36361 aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg
    36421 ttgataagtt cactggcggc ggggaagcta tccacgcgga tacggctttt ggtttgttga
    36481 tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc
    36541 cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc
    36601 atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg
    36661 tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct
    36721 catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg
    36781 ttacaacctg tagcatccac ttgtcggtcc tgtgagttaa tagggaatcc agtgcgaatc
    36841 tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat
    36901 ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc
    36961 ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt
    37021 cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt
    37081 cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa
    37141 ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga
    37201 tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga
    37261 tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc
    37321 cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg tgagtggttc
    37381 tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc
    37441 gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg
    37501 cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga
    37561 tgtctctacg cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc
    37621 ccatactcat ggttatgatg ttgttgccta tggtaataca ggaacgcaag cgcagacaga
    37681 taacgatggt tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc
    37741 ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta
    37801 ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg
    37861 gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa
    37921 agattacaac tacgatcccc attatggtcg ttatgattcg tcggcgacgc tcgatgagat
    37981 gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc
    38041 gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg aggatggata
    38101 tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg attttacctt
    38161 tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac
    38221 cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta
    38281 taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga
    38341 gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat
    38401 ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata
    38461 ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg
    38521 accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac
    38581 gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga
    38641 cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc
    38701 ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta
    38761 tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta
    38821 tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac
    38881 cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct
    38941 atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg
    39001 ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg gcaattgtca
    39061 ccgcggtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac actgccagta
    39121 ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg
    39181 cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg
    39241 gaacagttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga
    39301 tagaaatgct gggctcggca gagatggttg agttggctga agcgaagcta catggcgaag
    39361 atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc
    39421 cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa ctgttacaag
    39481 tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc cgaacggcct
    39541 ggttgttaga acatgaagcc ccggatgcaa aatctgccga tgcgaatatt gccttttgta
    39601 tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa
    39661 cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta
    39721 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    39781 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaatctca acagagaaaa
    39841 gcaaaaaaaa tgcttgactc tgtagcggga aagcgtatta tgcacacc
//
LOCUS       DAZOYS010000018        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOYS010000018 DAZOYS010000000
VERSION     DAZOYS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.018"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUN9_000794"
     CDS             59..1423
                     /locus_tag="ACHUN9_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694280.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUN9_000795"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUN9_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0694281.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUN9_000796"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUN9_000796"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0694282.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUN9_000797"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUN9_000797"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0694283.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUN9_000798"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUN9_000798"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0694284.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUN9_000799"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUN9_000799"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0694285.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUN9_000800"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUN9_000800"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0694286.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUN9_000801"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUN9_000801"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0694287.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUN9_000802"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUN9_000802"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0694288.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUN9_000803"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUN9_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0694289.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUN9_000804"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUN9_000804"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0694290.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUN9_000805"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUN9_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0694291.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUN9_000806"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUN9_000806"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0694292.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUN9_000807"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUN9_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0694293.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUN9_000808"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUN9_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0694294.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUN9_000809"
     CDS             13749..14288
                     /locus_tag="ACHUN9_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0694295.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUN9_000810"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUN9_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0694296.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUN9_000811"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUN9_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0694297.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUN9_000812"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUN9_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0694298.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUN9_000813"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUN9_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0694299.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUN9_000814"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUN9_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0694300.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUN9_000815"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUN9_000815"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0694301.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUN9_000816"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUN9_000816"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0694302.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUN9_000817"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUN9_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0694303.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUN9_000818"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUN9_000818"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0694304.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUN9_000819"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUN9_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0694305.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUN9_000820"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUN9_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0694306.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUN9_000821"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUN9_000821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0694307.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUN9_000822"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUN9_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0694308.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUN9_000823"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUN9_000823"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0694309.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUN9_000824"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUN9_000824"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0694310.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUN9_000825"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUN9_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0694311.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUN9_000826"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUN9_000826"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0694312.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUN9_000827"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUN9_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0694313.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUN9_000828"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUN9_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694314.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUN9_000829"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUN9_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9466 a        10313 c         9734 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZOYS010000019        37490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOYS010000019 DAZOYS010000000
VERSION     DAZOYS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.019"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUN9_000830"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUN9_000830"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0694315.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUN9_000831"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUN9_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0694316.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUN9_000832"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUN9_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0694317.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUN9_000833"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUN9_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0694318.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUN9_000834"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUN9_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0694319.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHUN9_000835"
     CDS             5280..5738
                     /locus_tag="ACHUN9_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0694320.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHUN9_000836"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHUN9_000836"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0694321.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHUN9_000837"
     tRNA            6790..6874
                     /locus_tag="ACHUN9_000837"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHUN9_000838"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHUN9_000838"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUN9_000839"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUN9_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0694322.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUN9_000840"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUN9_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0694323.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUN9_000841"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUN9_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0694324.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHUN9_000842"
     CDS             complement(10156..13899)
                     /locus_tag="ACHUN9_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0694325.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUN9_000843"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUN9_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0694326.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHUN9_000844"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHUN9_000844"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0694327.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHUN9_000845"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHUN9_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0694328.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUN9_000846"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUN9_000846"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0694329.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUN9_000847"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUN9_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0694330.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUN9_000848"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUN9_000848"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0694331.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUN9_000849"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUN9_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0694332.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUN9_000850"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUN9_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0694333.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHUN9_000851"
     CDS             23294..23401
                     /locus_tag="ACHUN9_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0694334.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..23937
                     /locus_tag="ACHUN9_000852"
     CDS             23830..23937
                     /locus_tag="ACHUN9_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0694335.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24365..24472
                     /locus_tag="ACHUN9_000853"
     CDS             24365..24472
                     /locus_tag="ACHUN9_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0694336.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUN9_000854"
     CDS             complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUN9_000854"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0694337.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25511..26320)
                     /gene="sirB1"
                     /locus_tag="ACHUN9_000855"
     CDS             complement(25511..26320)
                     /gene="sirB1"
                     /locus_tag="ACHUN9_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR0694338.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(26324..26716)
                     /gene="sirB2"
                     /locus_tag="ACHUN9_000856"
     CDS             complement(26324..26716)
                     /gene="sirB2"
                     /locus_tag="ACHUN9_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0694339.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="ACHUN9_000857"
     CDS             complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="ACHUN9_000857"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0694340.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="ACHUN9_000858"
     CDS             complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="ACHUN9_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0694341.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="ACHUN9_000859"
     CDS             complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="ACHUN9_000859"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0694342.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30140..30763
                     /gene="lolB"
                     /locus_tag="ACHUN9_000860"
     CDS             30140..30763
                     /gene="lolB"
                     /locus_tag="ACHUN9_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0694343.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30763..31614
                     /gene="ispE"
                     /locus_tag="ACHUN9_000861"
     CDS             30763..31614
                     /gene="ispE"
                     /locus_tag="ACHUN9_000861"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0694344.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31765..32712
                     /gene="prs"
                     /locus_tag="ACHUN9_000862"
     CDS             31765..32712
                     /gene="prs"
                     /locus_tag="ACHUN9_000862"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0694345.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32836..34515
                     /gene="dauA"
                     /locus_tag="ACHUN9_000863"
     CDS             32836..34515
                     /gene="dauA"
                     /locus_tag="ACHUN9_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0694346.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKTNSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="ACHUN9_000864"
     CDS             complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="ACHUN9_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0694347.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35125..35709
                     /gene="pth"
                     /locus_tag="ACHUN9_000865"
     CDS             35125..35709
                     /gene="pth"
                     /locus_tag="ACHUN9_000865"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0694348.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35826..36917
                     /gene="ychF"
                     /locus_tag="ACHUN9_000866"
     CDS             35826..36917
                     /gene="ychF"
                     /locus_tag="ACHUN9_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0694349.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9297 a         9740 c         9180 g         9273 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg gaagtaaggt
    23941 gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    24001 cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca acccgagctg
    24061 gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa ccggaatgca
    24121 ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta gtatccacat
    24181 caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    24241 ttgctggaaa gagaaaaccc ccgcacgttg agaggtttaa cctgacaaca gaacgggggc
    24301 taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
    24361 ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct
    24421 ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
    24481 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
    24541 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaagactt
    24601 tatttttcaa gcaaaaaaga ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
    24661 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
    24721 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
    24781 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
    24841 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
    24901 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaggtt gtcatagccg
    24961 aagttagcac cgcgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
    25021 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
    25081 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
    25141 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
    25201 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
    25261 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
    25321 taagggatac ccagtttctg ggtcacggtt acgtagtgct cgcaaatgcg catcgccaga
    25381 tcgcgagatt ccagcacgtt cataccacca aacagtacga acgacaggtc atttgctacg
    25441 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
    25501 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcacgga
    25561 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
    25621 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
    25681 cttccgggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
    25741 cttccatcaa cgaggctttg agtgtatcca acaatttgcg gattacctca atgttatcag
    25801 cttcatcaag gtcttcataa aacagttccg ccgacgggct gatatttccc tttaaccata
    25861 cgtccagcat atgttcgctt aacgattcac cgttaaaagg attaatcagc caaatttcgc
    25921 catccggaca ttcaatgcgc aatatcagct gcgtagggaa aatcaccggc agcagcggca
    25981 aatcgagacg attcgcgacc cataataaaa ccgcacctaa tgataccgca ctgccctgtc
    26041 gattctttaa cacctggtcc agccataatg catcggaaag acgataaacg ccgcgtgagg
    26101 ctttaaatcc ccagtcgccg taaaacagcg cgatcagttt ttccagctgc tcttctaaag
    26161 gcagaagctg gctgatttct tccttcgcca gactaacgag acgctccagt tcgtcgtaaa
    26221 catcttgcga gggaaaatcg cggcggattg cttcgcaagc caggatcatg ccttcgcaca
    26281 atggcgcttt attaaattcg aaatcagcta acgatctcat gacttacccc agtaacggta
    26341 cttttgtggt ggcgagttta atgatgatgt acagcaccac caacgccagc gggaaggcaa
    26401 taatgcgcgc ctgctgactg tgcatacgac gataatcgag tgcaataaaa cccaaaacga
    26461 tataaatgat aactccaaac agcttttcag tcagccactg tgcctgcccg gaaaatggca
    26521 ggatgtgcgc tttagccatc aacgcaatgc cgcttaacaa taacaccgta tcgacaaccg
    26581 gcggcacaat gcgcgtccag cgagcaaatg cctgtggatg cttctgataa cgtagccaga
    26641 aacgtaaggt taatagccca acagaaagcg cgatactaat aagatgaaca ctaagcagtg
    26701 tagaaaaact tgtcattgat aatagcggcc gagcgttacg cgctcgttat caccatagtc
    26761 acggcaggtt tcgacgtcat gataccccgc gaggataaat gcttgtcgca ccgcttcgcc
    26821 ctgctgccag ccatgttcca gaagcagaaa gccgccggat accagcgcgt tacgcgactg
    26881 ttcgatgata tgcacgatgt ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg
    26941 ctcaaagcgg acatcgcctt gttgaagatg tgggtcctgc tcgtcaatat acggcggatt
    27001 gctgacaatc atcgcaaact gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat
    27061 gtggatattt ttgatcgcca gatgctgggc attacgttgt gccagggaga cagcatcagg
    27121 catacgatct acagcgataa tttcgcagtc cgggcgctcg ctagccagcg caagcgcaat
    27181 cgccccggta cccgtcccga gatcgagaat acggcaaggt tgttcaggca accgcgccag
    27241 tgcctgctcc accagacact ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa
    27301 taacggcaac gaccagaatt ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg
    27361 acgacgtgtc agtagcgcat caagttgctg acattgttcg tcagtcagct gcgtttcacc
    27421 aaaggccaga ataaaagtcc gccccctgcc agtgacatac tccaataaga tttcagcgtc
    27481 acggcgtggg ctttcgctcg cctgaagttg gcttattgct tcacgtaacc agtattgata
    27541 ttccattatt cctgctcgga caacgccgcc agttggtcgg cctgatgttc ctggataatc
    27601 ggttcaatca gcatatccag cttaccttcc atcacttcat ccagacggta gagcgtcagg
    27661 ttgatgcggt gatcggtaac gcgcccctgc gggaagttgt aagtacggtt acggtcgctg
    27721 cgatcgccac tccccagcag gttacgacgg gtagacgctt cggcctgttg ccgttttgcc
    27781 atttctgcag cgtggatacg agcaccaagt acagaaagtg ctttagcttt gtttttatgt
    27841 tgtgaacgtt cgtcctgaca ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt
    27901 gccgaatcgg tggtgttaac gtgctgacca cccgcccctg acgagcggaa agtatcaatg
    27961 cgtaaatctg ctgggttgat gtccggcagt tctgcgtcag gcagttctgg cattaccgca
    28021 acggtacaag cagaagtatg aatacgaccc tgcgattccg tagcaggaac acgctgcacg
    28081 cgatgaccgc cggattcaaa tttcagacga ccatacacac catcaccgct aattttggcg
    28141 atgatctctt tataaccacc atgttcaccc tcgctggcgc tcatgatttc tacccgccag
    28201 cggcgggctt cggcataacg gctgtacata cggaacagat cgcccgcgaa cagcgccgct
    28261 tcgtcgccgc cggttccggc tcggacttcg aggaaggcgt tacgttcgtc atcaggatct
    28321 tttggcagta acagaacctg taattgctgt tccagttgct cgcttttttc tttagcttcg
    28381 cgcagttcat cctgcgccat ctcacgcatt tcaggatcat cgagcatcat ctgtgcggtt
    28441 tcgatatctt cctgaacctg ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc
    28501 gcatattcgc gtgataatgc gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc
    28561 agcaacgcct gaacttcttc atggcgttca tgcagggctt ccagtttggc aacgatagaa
    28621 ggcttcatag gcgtaaatgc accctgtaaa aaaagaaaat gatgtactgc tactccagcc
    28681 cgaggctgtc gcgcagaata ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa
    28741 gtgatttcgt tggcgcatgg atcaagcggt tagtcagttt ccatgccaga tcctgcataa
    28801 tggcttgcgc gtcgccgccc tgctcaaggg ccgctaacgc tttggcggtt aactcatcgc
    28861 gaacgtgctc tgcctggctg cgatactcgc gaatggtttc gctggcgctt tgtgctcgca
    28921 gccacgccat aaattcgctg gtttcctgag cgacaatagt ttccgcctca accgctgcgg
    28981 ctttacgctg cgccaggttg tgcgaaatga tgctttgcag atcgtccaca ctataaagat
    29041 aagcattcgc cagtttgcca acttccggct caacatcgcg cggaacggca atatccacca
    29101 acagcattgg ttggttacga cgacttttta atgcgcgctc caccatgcct ttcccgataa
    29161 tcggtaacgg gctggcggtg gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga
    29221 tctcactcag ggcaatcact tccgcgccaa cttcatctgc cagaatttgg gcacgttcgc
    29281 gagtgcggtt ggcgataatc atcttctgta ctttatgttc gcgcagatga cgcgctacca
    29341 gctcgatggt ttcgcccgcg cctaccagca acactgtgac cgtagagagc gattcaaaga
    29401 tctgccgcgc cagcgtacaa gccgcaaaag cgacagacac agcactggca ccgatatctg
    29461 tttcagtgcg aacgcgtttc gctacagaga aagatttctg gaacatgcgt tccagttcgc
    29521 tggccttcat atgacctttt tgcgaatcgg caaacgcttt tttaacctga ccgaggatct
    29581 gcggctcccc aagaaccagt gaatccaggc cgctggcaac acgcattaaa tggctaaccg
    29641 cgtcgttatc ctgatgccag tagaggcttt tacgcagatc ttcttcatta agattgtgat
    29701 aatcgcaaag ccagcggatt aacgcctctt gcaggttatc ctgctcttca acgctaagat
    29761 aaagttccgt gcggttgcac gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa
    29821 gcaggctgtc aagcgcctga tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg
    29881 atacaggtgc cgttttatgg ttgataccga gtgctaaaag ggtcatgtct gcgggaaata
    29941 ataccaacgt tgatatggtt agtctgcttg catcatacag gatgcgtaag atcaataaaa
    30001 gagagcgccc ccttttggag taattgccga aagccgttag attctggcaa ttaagacaac
    30061 ttgaacatag acgatagcgg acggtaacgc tagcattaag ggttataact gcaacgtatc
    30121 tcaaggactt gtcatcacta tgcccctgcc cgattttcgt cttatccgcc tgctaccgct
    30181 ggctgctctt gtgctcactg cctgttccgt taccacgccc aaaggtcctg gcaaaagccc
    30241 ggattcgcca caatggcgtc agcatcagca agacgtgcgc aatcttaatc agtatcagac
    30301 tcgcggcgcg ttcgcttata tttctgacca acaaaaagtg tacgcccgct ttttctggca
    30361 gcaaaccggc caggatcgct accgtctgct gctcactaac ccattgggca gcacggaact
    30421 ggagctgaat gctcaaccgg gtaacgtgca gttagtcgac aataaaggtc agcgttatac
    30481 cgccgatgac gccgaagaga tgattggcaa attgaccgga atgccaattc cgctcaacag
    30541 cttgcgccaa tggattttag gtttaccggg tgatgcaacc gactacaaac tggacgacca
    30601 gtaccgcctg agcgaaatta cctacagcca gaatggcaaa aactggaagg ttgtttatgg
    30661 tggttatgac accaaaacgc aacctgcgat gccagccaat atggaactca ccgacggtgg
    30721 tcaacgcatc aagttaaaaa tggataactg gatagtgaaa taatgcggac acagtggccc
    30781 tctccggcaa aacttaatct gtttttatac attaccggtc agcgtgcgga tggttaccac
    30841 acgctgcaaa cgctgtttca gtttcttgat tacggcgaca ccatcagcat tgagcttcgt
    30901 gacgatgggg atattcgtct gttaacgccc gttgaaggcg tggaacatga agataacctg
    30961 atcgttcgcg cagcgcggtt gttgatgaaa actgcggcag acagcgggcg tcttccgacg
    31021 ggaagcggtg cgaatatcag cattgacaag cgtttgccga tgggcggcgg tctcggcggt
    31081 ggttcatcca atgccgcgac ggtcctggtg gcattaaatc atctctggca atgcgggcta
    31141 agcatggatg agctggcgga aatggggctg acgctgggcg cagatgttcc tgtctttgtt
    31201 cgggggcatg ccgcgtttgc cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca
    31261 gagaagtggt atctggtggc gcaccctggt gtaagtattc cgacaccagt gatttttaaa
    31321 gatcctgaac tcccgcgcaa tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa
    31381 ttcagcaatg attgcgaggt tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt
    31441 tcctggctgt tagaatacgc cccgtcgcgc ctgactggga caggggcctg tgtctttgct
    31501 gaatttgata cagagtctga agcccgccag gtgctagagc aagccccgga atggctcaat
    31561 ggctttgtgg cgaaaggtgt taatctttcc ccattgcaca gagccatgct ttaagccggg
    31621 caagctgagt ttcggtgaca acgtcacctt gttccagacg ttgcatcgcg ctctttaata
    31681 caccgcctgg aaaggatcat gcctggcccg cacagttttc ggcagattct ttccaccaat
    31741 ggacgcatgc ctgaggttct tctcgtgcct gatatgaagc tttttgctgg taacgccacc
    31801 ccggaactag cacaacgtat tgccaaccgc ctgtacactt cactcggcga cgccgctgta
    31861 ggtcgcttta gcgacggcga agtcagtgta caaatcaacg aaaatgtacg cggtggtgat
    31921 attttcatca tccagtccac ttgtgcccct actaacgaca acctgatgga attagtcgtt
    31981 atggttgatg ccctgcgtcg tgcttccgca ggtcgtatca ccgctgttat cccctacttt
    32041 ggctatgcgc gccaggaccg tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg
    32101 gttgcagact tcctctccag cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct
    32161 gaacagattc agggtttctt cgacgttccg gttgataacg tatttggtag cccgatcctg
    32221 ctggaagaca tgctgcagct gaatctggat aacccaattg tggtttctcc ggacatcggc
    32281 ggcgttgtgc gtgcccgcgc tatcgctaag ctgctgaacg ataccgatat ggcaatcatc
    32341 gacaaacgtc gtccgcgtgc gaacgtttcc caggtgatgc atatcatcgg tgacgttgca
    32401 ggtcgtgact gcgtactggt cgatgatatg atcgacactg gcggtacgct gtgtaaagct
    32461 gctgaagctc tgaaagaacg tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc
    32521 ttctctggca acgcggcgaa caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc
    32581 gataccattc cgctgagcga tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg
    32641 tcaggtatgc tggccgaagc gattcgtcgt atcagcaacg aagaatcgat ctctgccatg
    32701 ttcgaacact aatcgaaccc gctcaaagac ccgctgcggc gggttttttt gtctgtaata
    32761 tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac
    32821 ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg
    32881 atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc
    32941 gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt
    33001 gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctgacg
    33061 ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc
    33121 gtgtcgcaac agtttggact ggcaggactg ctggttgcga ccttgctgtc ggggatcttt
    33181 ttgattctga tgggtctggc acgctttggt cgcctgattg agtatattcc ggtttccgtc
    33241 accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt
    33301 ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt
    33361 atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac gctaggtatt
    33421 cttgtttttt ggccgcgtct gggcattcgt ttacccggtc accttccggc cttgctggct
    33481 ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg
    33541 caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg
    33601 caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt
    33661 cgcacactgc tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga atctctgctc
    33721 tgcgccgtgg tactggatgg tatgaccggg acgaaacaca agacgaacag cgaactggtt
    33781 ggacagggac tggggaatat tatcgctccg ttctttggtg gtattaccgc tacagctgcc
    33841 atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc
    33901 cactctattc tggttattct tgccctgctg gtactggcac cgctgctctc ctggctgccg
    33961 ctttccgcta tggcagccct gctgttgatg gtggcgtgga acatgagtga agcgcataaa
    34021 gtggtcgact tgctgcgtca tgcaccgaaa gatgacatca ttgtcatgct gctgtgcatg
    34081 tcgctgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt gctggcatcg
    34141 ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagatgtt
    34201 ccagacgatg ttctggtact gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc
    34261 ttgttcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat
    34321 gccgttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg
    34381 cccgaaggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg cactatggct
    34441 cgcgcaggca ttcaaccgat cccgggacgc ctcgcgttct tcccgaatcg tcgcgcggcg
    34501 atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg
    34561 cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtgacg
    34621 aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca
    34681 gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact ggggcatatt
    34741 aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta ccaccagccc
    34801 caaccccatg agcacgttac cgagtaacga agcgtttttg cgtttcatat gtcacctccg
    34861 gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca
    34921 ccctgtgggt tatgtgcggg cgtgatcaca attacaaccc ttatttcaac aaaactttac
    34981 aaataaacgc ctgaacacct tgactttcta atagtccact caggcaatta ttacgttatc
    35041 tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt
    35101 atgtacacac tcaggacaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc
    35161 ggtgctgaat acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca
    35221 gagcgtttgc gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc
    35281 actcttggag gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc
    35341 aaagccgttg cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc
    35401 cacgacgaac tggatctgcc tcctggcgtc gccaaattta aattgggcgg tggccatggt
    35461 ggtcacaatg gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt
    35521 ttacgcatcg gaatcggtca tcctggcgat aaaaataaag ttgtcggttt tgtgttaggc
    35581 aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt
    35641 actgaaatgt ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa
    35701 gcgcaataag tcgttgtctg cggcattttt gccgagtgcc gtgtataata ggcaaagtta
    35761 tttccatttc tgcaatctgt tagcaataac agattgatta ttaagatatt aaggtgattt
    35821 aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt
    35881 tcaacgcgct gaccaaagcc ggtattgaag cggccaactt tccattctgc accattgagc
    35941 cgaacacagg cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa
    36001 aaccgcaacg tacgcttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag
    36061 gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag
    36121 cgatcggtca cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag
    36181 ttaacccggc tgacgatatt gaagttatca acaccgaact ggcgctggca gacctcgaca
    36241 cctgcgaacg tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aaagacgcga
    36301 aagctgagct ggcggtccta gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc
    36361 gcgcgctgga tttaagcgct gaagagaaag cggctattcg ttacctgagc ttcctgacgc
    36421 tgaaaccaac aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccatatc
    36481 ttgaccaggt gcgtgaaatc gcggcgaaag aaggttctgt tgtggttccg gtttgtgctg
    36541 ctgttgaagc agacattgcc gaactggacg acgaagaacg tgacgagttt atgcaggagc
    36601 ttgggctgga agaaccgggc ctgaaccgtg tgatccgtgt cggttataag ctgctgaacc
    36661 tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggag
    36721 caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg
    36781 cacaaaccat ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaagaag
    36841 caggcaaaat gcgtgcagaa ggtaaagatt acatcgttaa agatggcgat gtgatgaact
    36901 tccttttcaa cgtctaatta atttattgtc tcatgaggtt tcgctaaatc tcatgaagag
    36961 cgcaaaagta tataaaatcc acgcaattgc gtggattttt catttcaccg agtcctaatc
    37021 attcttgtaa catcctacac ttttgtgctt attgcctaca gtgaaccacg agaataatta
    37081 aagacatcag tgtcagatga cggatatctc attagtagtg atataacgta agtttttgtt
    37141 caactacaca tcaccccctt cacagctgat caatattgat cacgctccaa tatgctggcg
    37201 agaaaaggac ttacctgaag ggtaatatcc cgcagataaa gtccccgaat aacgtgatta
    37261 cgtgcgatcc tggcaagatg aaaatgataa ccagtagcac aataacaacg ataggccaga
    37321 aaacccattg accaaaaatg aatatcgatg ttttttgctt tttcttatta gtaatgcaaa
    37381 aatggcgcat attgtttttg ttatgcagat acatcaagcg aaattcccat aactaattag
    37441 gattattaca aaaaatcttc accaaatttt ttaaccgctg tttgttgtaa
//
LOCUS       DAZOYS010000020        36751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOYS010000020 DAZOYS010000000
VERSION     DAZOYS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.020"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUN9_000867"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUN9_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0694350.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUN9_000868"
     CDS             complement(280..873)
                     /locus_tag="ACHUN9_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0694351.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIAKRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUN9_000869"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUN9_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0694352.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUN9_000870"
     CDS             1788..2183
                     /locus_tag="ACHUN9_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0694353.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUN9_000871"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUN9_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0694354.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUN9_000872"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUN9_000872"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0694355.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUN9_000873"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUN9_000873"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0694356.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUN9_000874"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUN9_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0694357.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUN9_000875"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUN9_000875"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0694358.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUN9_000876"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUN9_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0694359.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUN9_000877"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUN9_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0694360.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUN9_000878"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUN9_000878"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0694361.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUN9_000879"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUN9_000879"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0694362.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUN9_000880"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUN9_000880"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0694363.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUN9_000881"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUN9_000881"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0694364.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUN9_000882"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUN9_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0694365.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUN9_000883"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUN9_000883"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0694366.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUN9_000884"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUN9_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0694367.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUN9_000885"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUN9_000885"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0694368.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUN9_000886"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUN9_000886"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0694369.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUN9_000887"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUN9_000887"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0694370.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUN9_000888"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUN9_000888"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0694371.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUN9_000889"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUN9_000889"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0694372.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUN9_000890"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUN9_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0694373.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUN9_000891"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUN9_000891"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0694374.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUN9_000892"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUN9_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0694375.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUN9_000893"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUN9_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0694376.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUN9_000894"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUN9_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0694377.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUN9_000895"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUN9_000895"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0694378.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUN9_000896"
     CDS             33227..34570
                     /locus_tag="ACHUN9_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0694379.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUN9_000897"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUN9_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0694380.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUN9_000898"
     CDS             35181..35735
                     /locus_tag="ACHUN9_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0694381.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHUN9_000899"
     CDS             complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHUN9_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0694382.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8903 a         9847 c         9135 g         8866 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcttggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaagaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tctcgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccgtt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt t
//
LOCUS       DAZOYS010000021        35990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOYS010000021 DAZOYS010000000
VERSION     DAZOYS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.021"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            31..594
                     /gene="srlA"
                     /locus_tag="ACHUN9_000900"
                     /gene_synonym="gutA"
     CDS             31..594
                     /gene="srlA"
                     /locus_tag="ACHUN9_000900"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0694383.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            591..1550
                     /gene="srlE"
                     /locus_tag="ACHUN9_000901"
                     /gene_synonym="gutE"
     CDS             591..1550
                     /gene="srlE"
                     /locus_tag="ACHUN9_000901"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0694384.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            1561..1932
                     /gene="srlB"
                     /locus_tag="ACHUN9_000902"
                     /gene_synonym="gutB"
     CDS             1561..1932
                     /gene="srlB"
                     /locus_tag="ACHUN9_000902"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0694385.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            1936..2704
                     /gene="srlD"
                     /locus_tag="ACHUN9_000903"
                     /pseudo
     CDS             1936..2704
                     /gene="srlD"
                     /locus_tag="ACHUN9_000903"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            2810..3169
                     /gene="gutM"
                     /locus_tag="ACHUN9_000904"
     CDS             2810..3169
                     /gene="gutM"
                     /locus_tag="ACHUN9_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0694386.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3236..4009
                     /gene="srlR"
                     /locus_tag="ACHUN9_000905"
                     /gene_synonym="gutR"
     CDS             3236..4009
                     /gene="srlR"
                     /locus_tag="ACHUN9_000905"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0694387.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4002..4967
                     /gene="gutQ"
                     /locus_tag="ACHUN9_000906"
     CDS             4002..4967
                     /gene="gutQ"
                     /locus_tag="ACHUN9_000906"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0694388.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(4964..6478)
                     /gene="norR"
                     /locus_tag="ACHUN9_000907"
     CDS             complement(4964..6478)
                     /gene="norR"
                     /locus_tag="ACHUN9_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0694389.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6665..8104
                     /gene="norV"
                     /locus_tag="ACHUN9_000908"
     CDS             6665..8104
                     /gene="norV"
                     /locus_tag="ACHUN9_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0694390.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8101..9234
                     /gene="norW"
                     /locus_tag="ACHUN9_000909"
     CDS             8101..9234
                     /gene="norW"
                     /locus_tag="ACHUN9_000909"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0694391.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9362..11614)
                     /gene="hypF"
                     /locus_tag="ACHUN9_000910"
     CDS             complement(9362..11614)
                     /gene="hypF"
                     /locus_tag="ACHUN9_000910"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0694392.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11767..12294)
                     /gene="hydN"
                     /locus_tag="ACHUN9_000911"
     CDS             complement(11767..12294)
                     /gene="hydN"
                     /locus_tag="ACHUN9_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0694393.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12442..13452)
                     /gene="ascG"
                     /locus_tag="ACHUN9_000912"
     CDS             complement(12442..13452)
                     /gene="ascG"
                     /locus_tag="ACHUN9_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0694394.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13712..15169
                     /gene="ascF"
                     /locus_tag="ACHUN9_000913"
     CDS             13712..15169
                     /gene="ascF"
                     /locus_tag="ACHUN9_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0694395.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            15178..16602
                     /gene="ascB"
                     /locus_tag="ACHUN9_000914"
     CDS             15178..16602
                     /gene="ascB"
                     /locus_tag="ACHUN9_000914"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0694396.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16716..17135)
                     /locus_tag="ACHUN9_000915"
     CDS             complement(16716..17135)
                     /locus_tag="ACHUN9_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0694397.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17132..17443)
                     /locus_tag="ACHUN9_000916"
     CDS             complement(17132..17443)
                     /locus_tag="ACHUN9_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0694398.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(17605..18378)
                     /locus_tag="ACHUN9_000917"
     CDS             complement(17605..18378)
                     /locus_tag="ACHUN9_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694399.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(18403..18873)
                     /gene="hycI"
                     /locus_tag="ACHUN9_000918"
     CDS             complement(18403..18873)
                     /gene="hycI"
                     /locus_tag="ACHUN9_000918"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0694400.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18866..19276)
                     /gene="hycH"
                     /locus_tag="ACHUN9_000919"
     CDS             complement(18866..19276)
                     /gene="hycH"
                     /locus_tag="ACHUN9_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0694401.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(19273..20040)
                     /gene="hycG"
                     /locus_tag="ACHUN9_000920"
     CDS             complement(19273..20040)
                     /gene="hycG"
                     /locus_tag="ACHUN9_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0694402.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(20040..20582)
                     /gene="hycF"
                     /locus_tag="ACHUN9_000921"
     CDS             complement(20040..20582)
                     /gene="hycF"
                     /locus_tag="ACHUN9_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0694403.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20592..22289)
                     /gene="hycE"
                     /locus_tag="ACHUN9_000922"
     CDS             complement(20592..22289)
                     /gene="hycE"
                     /locus_tag="ACHUN9_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0694404.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(22307..23230)
                     /gene="hycD"
                     /locus_tag="ACHUN9_000923"
     CDS             complement(22307..23230)
                     /gene="hycD"
                     /locus_tag="ACHUN9_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0694405.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(23233..25059)
                     /gene="hycC"
                     /locus_tag="ACHUN9_000924"
     CDS             complement(23233..25059)
                     /gene="hycC"
                     /locus_tag="ACHUN9_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0694406.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25056..25667)
                     /gene="hycB"
                     /locus_tag="ACHUN9_000925"
     CDS             complement(25056..25667)
                     /gene="hycB"
                     /locus_tag="ACHUN9_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0694407.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25792..26253)
                     /gene="hycA"
                     /locus_tag="ACHUN9_000926"
     CDS             complement(25792..26253)
                     /gene="hycA"
                     /locus_tag="ACHUN9_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0694408.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26465..26815
                     /gene="hypA"
                     /locus_tag="ACHUN9_000927"
     CDS             26465..26815
                     /gene="hypA"
                     /locus_tag="ACHUN9_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0694409.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26819..27691
                     /gene="hypB"
                     /locus_tag="ACHUN9_000928"
     CDS             26819..27691
                     /gene="hypB"
                     /locus_tag="ACHUN9_000928"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0694410.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27682..27954
                     /gene="hypC"
                     /locus_tag="ACHUN9_000929"
     CDS             27682..27954
                     /gene="hypC"
                     /locus_tag="ACHUN9_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0694411.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            27954..29075
                     /gene="hypD"
                     /locus_tag="ACHUN9_000930"
     CDS             27954..29075
                     /gene="hypD"
                     /locus_tag="ACHUN9_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0694412.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            29072..30082
                     /gene="hypE"
                     /locus_tag="ACHUN9_000931"
     CDS             29072..30082
                     /gene="hypE"
                     /locus_tag="ACHUN9_000931"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0694413.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30156..32233
                     /gene="flhA"
                     /locus_tag="ACHUN9_000932"
                     /pseudo
     CDS             30156..32233
                     /gene="flhA"
                     /locus_tag="ACHUN9_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(32270..32623)
                     /locus_tag="ACHUN9_000933"
     CDS             complement(32270..32623)
                     /locus_tag="ACHUN9_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0694414.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32700..32834)
                     /locus_tag="ACHUN9_000934"
     CDS             complement(32700..32834)
                     /locus_tag="ACHUN9_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694415.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            32910..35471
                     /gene="mutS"
                     /locus_tag="ACHUN9_000935"
     CDS             32910..35471
                     /gene="mutS"
                     /locus_tag="ACHUN9_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0694416.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35577..35990
                     /locus_tag="ACHUN9_000936"
                     /pseudo
     CDS             35577..35990
                     /locus_tag="ACHUN9_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         8537 a         9843 c         9534 g         8076 t
ORIGIN      
        1 ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag
       61 tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt
      121 cttccgctgt tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag
      181 catcgtattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg
      241 ctaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt
      301 atgccggaaa agtacaaacc cagctactac gcggcagcct cttatagctg ccactcaatg
      361 aatggcctct ttccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc
      421 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagctatct gctggttggt
      481 ctggtcacca acttctttcg cggctgggtg accgatctga ccaccgccat ttttgagaaa
      541 aagatgggca ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta
      601 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
      661 gcaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac
      721 aacttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
      781 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac
      841 gtattcccac cattaatatc cactcgacgg gcaagtccag tccgctggcg cagtacattg
      901 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
      961 caccacaacc ctcttccgtg ggccgtgact atgacaccag caagaaaatc accgaacaaa
     1021 gcgatggttt actggcgaag gtgggaatgg gtatgggttc tgccgttgcc gtgttgtttc
     1081 aatctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcgttcg
     1141 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
     1201 ttgctccgct ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc
     1261 cgctgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
     1321 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg
     1381 ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
     1441 gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt gagccacttt ttaaccggcg
     1501 caccgactgt actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac
     1561 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
     1621 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
     1681 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca atttacgctc
     1741 gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa
     1801 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
     1861 catgtggcag gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
     1921 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
     1981 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
     2041 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aggaacagcg
     2101 tatagttttg gtgctgacgc caccagcgag caaagcgtcc tggcgctctc tcgtggggta
     2161 gatgaaatct ttggtcgcgt ggatttgctg gtctacagcg ccggaatagc aaaagcagcc
     2221 tttatcagcg acttccagct cggcgatttt gaccgtgaat ctggtgggtt atttcctgtg
     2281 tgcccgtgaa ttttctcgtt tgatgatccg cgacgggatt cagggtcgca ttattcagat
     2341 caactcgaaa tccggcaaag tgggcagcaa acacaactct ggctacagcg cagcgaaatt
     2401 tggtggcgtc gggctgactc aatcactggc gctggatctg gcggagtacg gcattacggt
     2461 gcattcactg atgctcggta acctgctgaa atcgcctatg ttccagtcac tgttacccca
     2521 atacgcgacc aagctgggta tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt
     2581 accgctcaaa cgcggctgcg attatcagga tgtgctgaat atgctgctgc tctatgccag
     2641 tcctaaggcg tcgtactgca ccggacagtc gatcaatgtc accggcggtc aggtgatgtt
     2701 ctaatcaaca gcggagatcc gttaaggatc tccgtgagac taatagaatg cctgatgcgc
     2761 tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta tggtatccgc
     2821 actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat
     2881 ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtgggccg
     2941 ttccagcggg cgctttaaac cacgggtcgt ggtcgccatc gccctggacg atcagcagcg
     3001 catcgtcgac accttgttta tgaaaggact gaccgtcttc gcacgaccgc aaaaaattcc
     3061 cgcaattacc ggtatgcatg cgggtgattt acagcccgat gtgatctttc cccatgatcc
     3121 actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga
     3181 atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa
     3241 acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt
     3301 tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat
     3361 tctggaacat gcaggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga
     3421 atccgatccg cctatcgatc ataaaacact catcaacacc cacaagaaag agctgatagc
     3481 agaagctgcc gttagtttta ttcatgatgg cgattcgatc attcttgatg ctggcagtac
     3541 cgttttgcag atggttcccc tgctctcgcg ctttaataac atcacggtga tgaccaacag
     3601 tctgcatatc gtcaatgcgc tatctgaact ggataacgaa caaactatcc tgatgccagg
     3661 cggaacgttt cgtaaaaaat cggcctcatt tcacggacag ctggcagaga atgccttcga
     3721 acatttcagt ttcgataaat tgtttatggg caccgacggc atcgatctca atgcgggcgt
     3781 aaccaccttt aacgaggttt ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt
     3841 gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct
     3901 tgaaagcgtc gataagctga ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct
     3961 ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga
     4021 acgcgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc
     4081 tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg
     4141 tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca cttgccagta
     4201 ccggcacatc ggcttttttt gtccatccgg cagaagcact gcacggcgat ctggggatga
     4261 ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc
     4321 tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga
     4381 cgtcaccgct gggcctggcg gcaaaagcgg tgctggatat ctccgtagaa cgcgaagcct
     4441 gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc
     4501 tggcgatggc ggtcatgcag gcgcgcggat ttaatgaaga agattttgcc cgctcccacc
     4561 cagccggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg cgccgtgacg
     4621 atgccatccc acaggtggcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc
     4681 gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta
     4741 ccgacggcga tttacgtcgc tggctggtag gcggcggcgc actcaccacg ccagttaatg
     4801 aagcgatgac gacaggcggc accacgttac aggcgcaaag tcgcgccatc gacgccaaag
     4861 agatcctgat gaagcgcaaa atcactgccg caccggtggt ggatgaaaac ggcaaactca
     4921 ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc ttcattccca
     4981 gacgtttcgc cagccgatgc aggttggcga cgtcggtttc cagcatccgc gcgcaggcag
     5041 cccagttatg atgattttgt gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg
     5101 tcgcttcacg caggttttgc ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg
     5161 tcacctcagg aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg
     5221 ctctcgccag cactaccgcc cgatgaatag catgttccag ttcgcgcacg ttccccggaa
     5281 aacgatagtg ttgcagtaaa tttcgcgctc cggcacttaa taccacgcgg gagagcccca
     5341 gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct
     5401 cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaag tcagcgcgaa
     5461 atcgccctgc catcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat
     5521 cgacccgcaa actacggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca
     5581 gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atccagaaac agagtgccgt
     5641 tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca
     5701 catgcccgaa caactcactt tccgccacac tttccggcag tgcagcacag ttgagataga
     5761 ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac
     5821 cggttcccgt ctcaccgctg atcaggacgt tgagatcgga cgccgccaca atctcaatct
     5881 cttttttcag ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt
     5941 caaacggcgt ggcatcgcct ggcatcatat tctggctttc cagttgttca atcagcaacg
     6001 cattgcttaa cgctcccgcc gccagcgcag caatcagccg tagctcttcg tcgctgaaaa
     6061 catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgcccgg
     6121 caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac
     6181 cgtcataggg atcgggcaat tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg
     6241 caatcgcttc cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg
     6301 ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg
     6361 catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac
     6421 caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct
     6481 ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat
     6541 tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc agataaaaaa
     6601 ctggcacgta atctgcaatt agcaagacat ctttttagaa cacgctgaat aaattgaggt
     6661 tgctatgtct attgtggtga aaaataacat tcattgggtt ggtcaacgtg actgggaagt
     6721 gcgtgatttt cacggtacgg aatataaaac gctacgcggc agcagttaca acagctacct
     6781 catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca gccgcgaatt
     6841 tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg tgattaacca
     6901 tgcagaagag gaccacgctg gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc
     6961 gatttactgt actgccaacg ctatcgactc gataaatggt catcatcatc atccggagtg
     7021 gaattttaat gtggtgaaaa ctggtgacac gctggatatc ggcaacggca aacagctcat
     7081 ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg atgacttacc tgacaggcga
     7141 cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc atctgttcaa
     7201 cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg ccaatatcct
     7261 gacgccgttc agccgcctgg taacaccgaa aattaccgag atcctgggct ttaacttacc
     7321 agtcgatatg atagcgacct ctcacggcgt ggtatggcgc gataacccga cgcaaattgt
     7381 cgagctgtac ctgaaatggg cggctgatta tcaggaagac agaatcacca ttttctacga
     7441 caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggga ttgcggaaac
     7501 cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa acgaaatcct
     7561 gaccaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga ataacgtgat
     7621 gatgccgaaa atcgccggtc tggtggagga gatgaccgga ttacgcttcc gtaacaaacg
     7681 cgccagtgct ttcggctctc acggctggag cggcggtgcg gtggatcgtc tttccacgcg
     7741 cctgcaggat gcgggtttcg aaatgtcgct tagcctgaaa gccaaatggc gaccagacca
     7801 ggacgctctg gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc
     7861 gccgctgccg cagagcacgg tgaatacggt ggttaaagaa gaaacctctg ccaccacgaa
     7921 gtccgacctc ggcccacgga tgcagtgcag cgtctgccag tggatttacg atccggcaaa
     7981 aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagttc cggataactt
     8041 cctctgcccg gaatgctctc tcggcaaaga cgtctttgac gaactggcat cggaggcaaa
     8101 atgagcaacg gcattgtgat cattggttcg ggcttcgccg cccgccaact ggtgaaaaat
     8161 attcgcaaac aggacgccag tattccatta accctgatcg ccgccgacag catggatgag
     8221 tacaacaaac cggacctcag ccatgttatc agccaggggc aacgtgccga cgaccttacc
     8281 cgccagacgg cgggtgaatt tgccgggcag tttaatctgc acctgtttcc gcacacctgg
     8341 gtgacggata tcgatgccga agcccatgtg gtgaaaagcc agaataatca gtggcaatac
     8401 gacaagctgg tgctggcaac tggtgccagt gcctttgtcc cgccagtgcc tgggcgtgag
     8461 ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat
     8521 gcccgacgcg tgttgattgt cggcggtggt ttgattggta gcgaactggc gatggatttt
     8581 tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcatta
     8641 atgccaccag aagtaagcag ccgcttgcag catcggttga cggagatggg cgttcatctg
     8701 ctgttgaaat ctcagttgca ggggctggaa aaaacggatt ctggcattct ggcaacgctg
     8761 gaccaccagc gtagtatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa
     8821 acagccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat
     8881 ctgcaaacca gtaatgacga tatttatgcg ctgggcgatt gcgcggaaat caacggtcag
     8941 gtactgccat tcctgcagcc gattcaactt agcgcgatgg tgctggcaaa aaatcttctc
     9001 ggcaataaca cgccgctgaa actaccggcg atgctggtga aaatcaaaac gccggaatta
     9061 ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcaaat taataccgaa
     9121 cgccagggaa tggtggctcg cggtgttgac gatgctgacc agcttcgcgc ctttgtggtc
     9181 agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa cattgccgat gtaggtgggc
     9241 tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca
     9301 tgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa tgcaacacgt
     9361 tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca
     9421 gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga
     9481 gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca
     9541 gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca
     9601 gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc agccactgct
     9661 gccagaaagc ggcgagatcc agttgattgt ccaccagcgg catcgtcacc ggatgcgtca
     9721 ctccgtggca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct tcataactta
     9781 acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacaaccgc ccacacgatg
     9841 acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagcacgctc cagttttgct
     9901 gttgcacact tgctgtttca gggtaattct gccactccgg cacaaagcgc aggcactgcg
     9961 ccagcaggtt tcgccacggc tgcttcgctg ccaaatcgcc acccggaagc gccactgcag
    10021 gcaagccgcc caggtgctca cattcgcgat agttcacccg caggcactcg ccgccccaca
    10081 aagcgccgtt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat
    10141 ccaacggcca ctgatgctct gccagacacg ccgctgcatg ggcatgatga tgcagtaccg
    10201 tttgcgtcgg cagattcatt tcacgcgccc actggctgga gacatagccc ggatgcgcgt
    10261 catgcacaac gtattgcggg gtaaaatcgt agatgttttg catcaggcgt aacgcttcgc
    10321 gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt
    10381 gttcaccgcg caccaggcag aaggtatttt tcagatccgc gccgagacac agcacaggcg
    10441 gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc cgcgaacggc
    10501 gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg atgtcgcggt
    10561 tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga
    10621 tagctggtgg tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct
    10681 gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg ttaaggtcag
    10741 gggcgatatc atcacaaagc tcaggaacgt attttttatc caccagcaca atcggcgcgg
    10801 cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact ggcaacatga
    10861 ccgccagcgg tttcgccggg cgatgtttgc gcgcccgaag tgtcgccacc gcgttactgt
    10921 tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca atgttgccca
    10981 tttttaactg tgcgatagcc gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac
    11041 ttacccattc aagatgtggg ccacactccg ggcaggccac cggctgggcg tggaagcgac
    11101 gatcgagcgg gtcacggtac tctttgtcac aagccggaca caacggaaac gccgccatca
    11161 cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg ccgcagtggg
    11221 tacagttgat aaacggataa cgataacgcc gttcgcctgg ggtattcatt tcggcaaggc
    11281 aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct cccgcgctct
    11341 ggcggatggt gaactcagtg ggcagttgtg accagatgta cggctcacgc tcgacgctat
    11401 caatacgcgc cagcggcggg cagtgctgat gcaattgaac aagtaacgtt tccgggtctt
    11461 cccgcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga agatttaatt
    11521 gctgtgccag ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac
    11581 gcagttggac accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg
    11641 cgtaacccgt cctgccggac acgactgcgt cgtatccggc agtctcaggt cggcgatacc
    11701 gccagctccg tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta
    11761 agatttttag aacatcagcg ccgtacggcg gcgtttttct gcactcagtt gttcaagttt
    11821 attacgatcg acacaaatca gcgcatgagt cgggcaagcc gccatacacg ccgggccgtc
    11881 ttcacgatgg ttgcacaggt cacatttatt ggcttcggct ttgtcagccc gtacattcag
    11941 ccccgcgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc catacgggca
    12001 agccacaacg caggttttgc aaccaatgca acgttcctgc atcacatgaa caaacccttt
    12061 atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg
    12121 gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt
    12181 ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc
    12241 acaggtacgg caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt
    12301 ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt ttaattcact
    12361 gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga cactagtgac
    12421 gatgacatgt gatgacaatg tttatcgcga aagagcaatg agtgagtcgc ggcggatcag
    12481 ttttccgctg aaggttttcg gcggtgagaa atccccgcca tcgagcataa aaatcagccg
    12541 tccaataatt tcctgaatca tctcagtcac cggaattttt acgctggaga gcgccggaac
    12601 gatatagggg gcaattgcga tatcatcgaa tccgataact gacacctgct ctggcaccgc
    12661 tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc atatcgtcgt tactggcaac
    12721 taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg ccccgctggc
    12781 gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat gctgcgccag
    12841 cgcgtcttta tacccagcaa gacgttcaat gctggtgggg gaatccatcg agccggtaag
    12901 gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcattaa agctggtctg
    12961 tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat taagcaccat
    13021 tatcggctga ctgtgcgcgt caatgatgtc atcgatctca tccacgctta aaaaacgcgg
    13081 gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc
    13141 ttcggcgctg tgtttaccat ctgccaatag caactgccgc cctttctctt ccgccattcg
    13201 cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt tggtcactac
    13261 caaccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt ttgggcggta
    13321 accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga cgtagccatt
    13381 acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag
    13441 catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact gcaccgtttg
    13501 ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc acaaatataa
    13561 aaaatgaaca attaactctc ttgcttattt agtgacaact attcatgatt ttgtgaaacc
    13621 ggtttcctaa ttccgtttca gcatcggcat ttttccgtta cgtcgactga taacaactac
    13681 atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg cgctggcacg
    13741 ctcggtgata gcggcactgg gcggcgttga taacatctcg gcggtcacgc actgtatgac
    13801 gcgtttgcgc gttgttatca aagatgacca gcttatcgac agcccgacgt taaaaaccat
    13861 ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta tcggcaatac
    13921 cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc agcccgcaca
    13981 gcccgtgggt aaacccaaac tcacgctgcg tcgcattggt gcggggatcc tcgatgcgct
    14041 gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg tcaaactgct
    14101 ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct taaccattct
    14161 gaatgtgatt ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg catctgccgc
    14221 catcaaattt aaaaccaata tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc
    14281 gagttttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat
    14341 tccggtcacc gcggtgaaat acacctacac agtgatcccg gcgctggtga tgacctggtg
    14401 cctgtcatat atcgaacgct gggtggatcg cattacaccg gcggtgacca aaaacttcct
    14461 caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga ttggcccgat
    14521 tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg gttatctggg
    14581 ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga ccgggatgca
    14641 ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag aagggatggt
    14701 catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg cggtggcgtg
    14761 gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat cagccattat
    14821 ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat
    14881 cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag
    14941 ccactcaatg gcagcgccag ggttatttac cagcgtgcaa ttcttcgatc cggcgaatcc
    15001 aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtggtgctgt cgtttatcct
    15061 cacactgttg ctcggctttg aggatattcc tgttgaggaa gcggctgccg aggcgcgaaa
    15121 gcatcagagc gtacaaccga ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg
    15181 tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca gtctgaaggt
    15241 gcgttccgtg aaggtggcaa agggctgacc actgtcgata tgatcccaca cggcgagcat
    15301 cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga cgagttttat
    15361 cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc cctgatggca
    15421 gagatgggat tcaaggtttt ccgtacctca attgcctgga gccgtctctt tccgcagggc
    15481 gatgaaatca cgcccaatca gcagggcatt gctttttatc gttctgtctt tgaagagtgt
    15541 aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc gatgcatctg
    15601 gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag ccgctacgcc
    15661 cggacctgct ttgaagcatt tgatggtctg gtgaaatact ggcttacctt caatgaaatc
    15721 aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga aggtgaaaat
    15781 caggatcagg tgaaatatca ggccgcgcat caccagctgg ttgccagtgc gctagccacc
    15841 aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc gggcggtaac
    15901 ttctaccctt acagttgcaa gccggaagat gtctgggcgg cgctggagaa agaccgggaa
    15961 aacctgtttt ttatcgatgt gcaggcgcgg ggcacgtatc cggcttactc tgcccgcgta
    16021 ttccgcgaaa aaggggtaac catcgacaaa gcaccgggcg atgatgaaat cctgaaaaac
    16081 accgtcgatt ttgtctcttt cagctattac gcctcgcgct gcgcctcggc ggagatgaac
    16141 gccaacaata gcagtgcggc gaacgtagtg aaatcgctgc gtaacccgta tctgcaagtg
    16201 agcgactggg gctggggtat tgatccactc ggtctgcgta tcaccatgaa catgatgtac
    16261 gaccgttatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa agatgaactt
    16321 gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga acatatccgc
    16381 gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac ctggggctgt
    16441 attgatttag tctccgcctc tacgggtgaa atgagcaaac gctacggttt tgtctacgta
    16501 gaccgtgacg acgcaggcaa cggcacgctg acgcgcacac gtaagaaatc gttctggtgg
    16561 tataaaaaag tgattgccag taatggggaa gatttagagt aggtaacagt gccggatgcg
    16621 gcgtgaacgc cttatccggc ctacatgtcg cacgaaccag gaaggcccga taagcgcagc
    16681 gcatcgggca atctggggtt tggcatcagt caaaatcaaa gaaaccattc cggcttaacg
    16741 ccaaatcggg ctgaaagtgc cttaatgtgg gatatcgtca gggatcgttg tccagacaaa
    16801 atctgactga ccagggattt agagccgatt tcgttcttca aatccgcata ggtcagatta
    16861 tgttgatcaa tcagcgtgcg aagtaacgcc acgccaacag gcattgccgc aacggctttg
    16921 tcgaactctg caaactcttg gttgttgttt tcatattctg caatgcggct cgccagaaag
    16981 tcgataagtg gattggtgtc atcttcttca atcaaatatt cgaccagttc cagtgcttca
    17041 cgataatcat cttctgagtc actacctccc agaaagggga ctgcattgac cagttctctt
    17101 gttgctttaa cagcgcgtgc agcattagca gtcattcttt gttctctctg tagtaacgtg
    17161 tcagtttgtc atactcggcg tgattagtta tgtgcttcac aaagaaccgc ttattaacaa
    17221 agtttatgta ggcaatcacc cttaggttgt tcccacctac atccagaacg taccatttgt
    17281 tgcgatattt aaaattgtcg aggttgggaa acgccgttcg catctcttct ggcgtagaaa
    17341 aatccgtttc cttaataacc cgatacaatg cctgaagagc cagagcgtcg ttaggatatt
    17401 tacgcgcaga ctcttcaaat ggcgctttag aaatgatgtg catttttaag ttttaacttc
    17461 cttatagtaa gtaaacagcc acatctgcga gttctatcct tatgtgttcg gaaactatag
    17521 ctcatcaaat ccaagttcac aatatgtaaa cacttgagcg cgatataatt acccccaaaa
    17581 tcctcatagc cactctgcaa aaagtcagtt ctggttcacc ccatcaagaa catccccctt
    17641 tatcaagcaa cgctgcgtct gcgccagatc ttccgtttgc gggtcgcgcc cggcgatggg
    17701 tttgaggacg agtttacggt tgggggtata agtaaaacgc gtgagcgcgg gatagcttaa
    17761 cttgccaaac cagccgtgaa cacgctgcca tttggcgtct tttacctgat acagactggt
    17821 tatccagtaa ccggaatcaa actgcatatc cagcaattgc gtgtgaccat tgccttgcaa
    17881 atccagcaaa tcatctacgc cagttttgct tgcggtgtag aatcccctgg gttcaaagac
    17941 acagggacgg ccttcgcgcg tgaacgtcat aagaattaaa tgtgcataag gagccagacc
    18001 attaccggta cttggtaacc agataaccag atcctgaatg ccgttacgat caagatcggc
    18061 gcgatagagt tgccctttgc ccgctatgtt tgccagcata cccacaggca cagaccagga
    18121 tttaccggcg cgatctttac cgttcaattg ccattcacta tcatcatttg gcatcttttc
    18181 aagcgtcacg ccagcaacgc ccacttttct aaaatgccag gtttgctcgg catcataagg
    18241 catggtcagg agaggtttca gcgcccgctt ttgcgcgtat tcgctgtcac tcattggcag
    18301 atattcggta aagctggtgg cctgtgcagg cagcactaag ctcaataaaa aagcgcacat
    18361 ccctgttctg atattcattt cgctgaattc cctgatggag aattattctt cttccaccgc
    18421 taactgcgcg aagccgccat tcccttccca gccttccagt cgttgataaa cggtttctac
    18481 cgcatcttta atcggctgag tcatcgggta gtaaaagccg acgatatccg gctggatgcc
    18541 gaggaaaatc acttcgccaa tgtcttcttt caactggtcg ataagatagt tcagcggcat
    18601 gttatgggta gtcatcataa acatctcggc gatatcatcc gggtcgatga tgcggatttc
    18661 gccggggttt agccccatat cagtagcgtc aacaatcagt aaccgcgtag ggcgcagttc
    18721 acggatggcg acgatgtcgt tttccggtgc actaccgcca tcaatcacca cccagttacc
    18781 tttcggcgcg gcggcgcact tttccgccag cagcgggcca gcgccatcat cgcccatcat
    18841 gctattgcca acacagagta aaacgtcagt cacgcagtct cctcaccatc aaatagatgg
    18901 cgttttcctg atgaatatca tgcagcatac ttaacagcat tgtactccat tccttctgct
    18961 gcgaagtttg cgctgcccgg gctttatcga atgcgttggc cagcatcggc acatggttaa
    19021 tgtcgatgac gatctcacca tatttcggca cgccttccat tttgcggcgg gcttcactgc
    19081 ccgcctccag agtggcaatc catgccagat attcatccca cgggcaggtg agcgccgctt
    19141 ccaggcaatc gataacccca aggtggtgac caatcgccag gctgtaatag accacctgct
    19201 gcgcctcggc gggcgtggca tcgttctcat caataaattt acggctcagt tgactgaaca
    19261 ccaccttttc actcatcgga tacgcgcctc ttcaacaaca tgattcagat ggctgacaat
    19321 ctcgttcaga cgcggatcgt tttccgcttc cagccagcgt gcaacctgtt cttcgccctg
    19381 ccctaactgt gtcaggaagt catcggcaat ctgacgaccg taacgataac ccgccaggcg
    19441 gcgtgcttcg cgatccactt tcacacgcag cggctgcacc atatcgccat gcaggatttc
    19501 cgcctgttgt tcatcctgct cacccgggcc acgggcgtga attttctgct ccagcaggcc
    19561 gagcgccatt gcaaagccgt acagcgtggc ggcaggcgtt ggcgggcaac caggaatgta
    19621 gacatctacc gggacgattt tatccgtacc gccccacacg cagtagagat cgtggaaaat
    19681 accaccgctg ttaccgcagg caccgtagga gatacaaatt ttcgggtccg gcgcggactg
    19741 ccacgcacgc agcgcagggg atcgcattgc acgggtgacc gcgccggtaa acagtaaaat
    19801 atccgcatga cgcggtgaag gaacgacttt aatgccgaag cgttctgcat caaacagcgg
    19861 cgaaagcgtg gcgaaaattt cgatttcgca gccgttgcag ccgccgcagt ccacgcgata
    19921 aacgtaggca gaacgtttga tttttttcag taacgacgcc ttcatgctgg cgatggattc
    19981 atccaccgtc atcgggaccg gaatgccgtt ggcgtcacgg gggcctaata aattgctcat
    20041 cagatggcct ctttcatatg gcgagtcagt tcaatacggt cggacggcac caggcatttc
    20101 tggcgcttac attccgggca agtctcaaag ctttcgcggt ggttttccgc gcggctgtcg
    20161 ccgttgtgct taagcagcgc aatggcgtag tcgatctctt tctggacggc gaaaggacga
    20221 ttgcagacgc ggcagttgca cagcgcgaag cgggactgtt gcaggaagtc ttctttcttc
    20281 cacactgcca gttcgtactc ttgcgacagt ttgatcgccg ccgtcgggca aacttcttcg
    20341 cagcgtccac agaagatgca gcgcccgaga ttaaactccc acgccagctc accggtggcg
    20401 aggtcagttt caaccgttaa ggcgtttgac gggcaggcat tgacgcaggc cgcacagccg
    20461 atgcactgct gcgggttctg ctccggctta ccacggaagt ttttatcaac cgcaatcggc
    20521 tccagcggat aagacgaggt cgccgtgccg gttttgatga cttttttgat aaaggtaaac
    20581 atggcgattc cttatttcag cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct
    20641 ttgtacggca ccactttgct ctttttctta cgcacatcga ccacggtcat gcggtcggta
    20701 caggagtagc aagggtccaa gctaccgata atcagcggcg catcggaaac ggtgttaccg
    20761 cgcagcatgt agcgcagggt cggccagttc gcgtaggtcg cggcacggca gcgccagcgg
    20821 tacagcttct ggttgtcgcc ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg
    20881 gcaaagccca gcgcgaaacg gtgcggaatg taggtaaagc cttccaccat cagcggaccg
    20941 cccggcaggt tatccagacc gtagtcgatc atgttcagcg cggtataaac ttcgttgata
    21001 cgcactttca gacgggaaat aacgtcgcag ccctgctcgc tgtggacttc cattggcagc
    21061 aggccataac cgacaaacgg gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga
    21121 accatcgggc caacgttact gaaatcgcgg gccatttccg ggtccagacg accaatgccg
    21181 acggtgcgct gttccatgtt cggcgtactc agcagcacat ccaccagctc ctgcacttca
    21241 cgacgcatct gttgcgccag ctggcgggtc tggatcatgt catctttcaa cagatcgcga
    21301 cgaatcccgc cgatcaggtt caggccgtag gttttacgcg caccggtgag gatctctgcc
    21361 attttcatgg aggtttcacg cacgcggaag aactgcataa agccggagtc aaagccggta
    21421 aagtggcagg cgagaccgag gttgagcaga tgcgagtgca ggcgttccac ctccagcaga
    21481 atggcgcgga tcatctgcgc acgttctggc acctgaatac ccatcgcgtt ttctaccgac
    21541 gtggtgtagg cggtgctgtg ggcaaagccg cagatcccgc acacacggtc agagaggaag
    21601 gtcacttcgt tgtaacccat acgggtttcc gccagttttt ccatgccgcg atgaacgtag
    21661 aacagacggt agtcggcgtc gataatgttt tcgccatcga cgaacagacg gaagtggccc
    21721 ggttcgtcag aagtgacgtg cagcggacca atcggcacga cgttgttttt cttgtcgccc
    21781 agttcgttga tgaactcgta ggtttcagca tcggtggtcg gtgccggacg ctgacgataa
    21841 tccatgctgt ctttacgcag cggataaagt tcatccggca gcaccagacg gcgttcatcc
    21901 ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc
    21961 gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcatcaac ttcgacgcga
    22021 accgttaccc agcacttagt ccccttctcc atcgacagca cgtaataaac ggcgtaatga
    22081 ccattcagtt tgcgttcgtc gttaccaaaa agcaccgaca accagccccc ctgcttgtag
    22141 tagagaaact ccaccacttc cggcagatag ttcaccttta cggtgacagt cagctgatct
    22201 ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag cgcggcgaga
    22261 taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca
    22321 aggagacgaa cgctaaaaat gcaaagccaa acccagtcca ggtaatgcgc ggagtaatat
    22381 caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgacgacca
    22441 gtttgacgat ggcaatcacc agcgccagca gcagcccacc ggcggtgaag gtttccatct
    22501 gtccccacgg gataaatacg ccgacgaaca tctgcagcac caccagctgt ttcaggctga
    22561 taccccattt catgacgcca aagccgctgc cgctgtattc agagagcggg ccttcctgca
    22621 gctcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaggtggcga
    22681 acgcacaggc acaaagcgcc agtaccagcg ggatgctctg gctcagcggc cagtgataaa
    22741 cggtgtcggt gatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac
    22801 caagcagcag catcggttcg accagcacgc caagcatcgc ttcacggctc gcgccgatag
    22861 cggtaaacgg gctaccggta tccagaccag aaatggcaaa gaagaaacgc gcgatggcaa
    22921 agagatacag taaggtgatt aaatcaccca gttgcggcag cggagaaccg acggtcacca
    22981 ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga
    23041 acacccagcc ggaggcatcc gggccaacgc tctggcggcc cagcagtttg aaaatgtcac
    23101 gatactcctg caacacaccc ggcccacgac ggttatgcag acgggcgcgc gccacgcggg
    23161 taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata
    23221 aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac
    23281 cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg gcaccagcga
    23341 caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt taaccggcat
    23401 ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc
    23461 cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg
    23521 cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc
    23581 aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg gcgcagcaac
    23641 accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc
    23701 acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac
    23761 tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg
    23821 ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg attgatagat
    23881 aacccattcc ccggcaaacc cgttcagtgg cggcagcgca gccattgcca tcaacccgac
    23941 taacatggcg atggagataa ccggcatttt cttgccaata ccaccgagtt tttcgatatc
    24001 gcgatgaccg gtacggaacc agacgctccc cgccccgagg aacagtacgc ttttgaacag
    24061 gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca gcgccggttg
    24121 ttcgagcgcg atacccgtta cgccagcgcc cagccccagc aggataatgc cgatattttc
    24181 cagggtgtgg taagccagca ggcgctggat gttgtgttcc atcaacgcat acagaccacc
    24241 gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt
    24301 accgcccagc agtgacaggg ttaacatgcc cagcaggcca attttcatga ctaccgtaga
    24361 aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag
    24421 cggaataatc ccggccagca ggccaaagcc aatcacaccg agcagccaga tatcggaacc
    24481 gagcggcagc tgttgcatac gcatatccag taggcacaga tccagcgtgc cataacgctg
    24541 ccacagcagc cagcaagcaa tcaccagcag cagagtgcca agacgcccca gcgcaaacca
    24601 cagtttgccc tctttgctgt tgctggtgag gaacaccgca cacaaggcca tgacttcggc
    24661 cattaccacg aacatgccga ggttgctggc gatgacggcg cagacggcgg cagccatcaa
    24721 catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta
    24781 gaggctgaca aacagaccgc acagaccgag cgtaatcagc caaatcgcgt taagcggaga
    24841 gatttgcaca tcgtagctta ccagcgacag cgcaccgctc acgccaaccg cgccagtcag
    24901 tacagtgaag cccgcggctg ccgtatacag actaccaacc gcgccgccaa ttccagctat
    24961 ccagccactt aacgcttttt gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25021 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25081 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25141 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25201 acgcaggccg gaccttgttc atcaaaactg caaaggtcac atttgacggc gatcgcgcga
    25261 atacccggca cccagtcaag cagcgtactg atacgcgccg gagcaggcgg tgccggtggc
    25321 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25381 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25441 tgcacggccc catcaatgcg ggtgatggcg ttaaccgggc aaaccaccgc acagggtgca
    25501 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattcagcat cactctcagg
    25561 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg
    25621 tggcagccga tacagagcgt ggagtcaaca attacaaaac gattcaccag gcattcctca
    25681 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    25741 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    25801 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatagcggt
    25861 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    25921 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    25981 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26041 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26101 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26161 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26221 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttacccgttt aacagagatg
    26281 ccagctttaa gcattttcta tgccaacttt taatttattg ttattaaata gatttttaag
    26341 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26401 cgcagggata atcaacactg gcacaattat tgcttgtagc tggcaatagt taatgggagg
    26461 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    26521 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    26581 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    26641 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    26701 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    26761 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    26821 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    26881 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgaaga tgaaaatcac
    26941 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27001 tcatggcgaa gcgggcactc acgcgccggg catgagccag cgtcggatgc tggaagtcga
    27061 aattgacgtg ctggacaaaa ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27121 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27181 gacggaaacc ctaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggtgacca
    27241 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc acaccagcga ttcaggtgaa
    27301 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27361 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27421 cttcgatctc ggtgaaaaac acaaagtggc ggtgctttcc gttaccgaag gtgaagacaa
    27481 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    27541 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    27601 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    27661 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    27721 ggcaaccagg caaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    27781 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt acacgttggc
    27841 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    27901 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    27961 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28021 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgcggtg
    28081 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28141 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28201 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtgc
    28261 cggggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28321 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgtaaa gtcgtattct
    28381 tcggcttagg ttttgaaacc accatgccga ccaccgccat caccctgcaa caggcgaaag
    28441 cccgcgatgt gcagaatttt tacttcttct gccagcatat tacgcttatc ccgacgctgc
    28501 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    28561 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    28621 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    28681 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    28741 acctgctggc gcaacaggca attgctgatg tgttctgtgt caacggcgac agcgaatggc
    28801 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    28861 atgccgaagc acatttccgc ccagcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    28921 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    28981 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29041 ggtatcagta tcgtcagcag gagaatgaag cgtgaataat atccaactcg cccacggcag
    29101 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29161 gtggctggca gagcaggaag accaggcacg tcttgatctg gcgcagctgg tagcagaagg
    29221 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29281 taatatcggc aagctggcga tttgcggcac agccaatgac gttgcggtca gtggcgctat
    29341 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29401 agccgtagtg accagcatgg cagaaaccgc gcgcgcggca ggcattgcca tcgttactgg
    29461 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    29521 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    29581 actggttagc ggcacgctcg gggaccacgg ggcgactatc cttaacctgc gtgagcagct
    29641 ggggctggat ggcgaactgg tcagcgactg cgcggtactg acgccgctta ttcagacgct
    29701 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg tgaacgcggt
    29761 agttcatgag ttcgcggcag cctgcggttg tggtattgaa atttcagaag tggcactgcc
    29821 ggttaaacct gccgtgcgcg gcgtttgcga actgctggga ctggacgccc ttaactttgc
    29881 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    29941 gttacattcc catccactgg ggaaagacgc ggcgctaatt ggtgaagtgg tggaacgtaa
    30001 aggtgttcgt cttgccggtc tgtatggtgt gaaacgaacc ctcgatttac cacacgccga
    30061 acctcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30121 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30181 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30241 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30301 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca
    30361 ccccattaaa tatgaagacg aaactgttct ggcacacggt ccggtacgcc gcattttgtc
    30421 gcgccctgat acgctgcatt gcagttacga agaattttgt gaaacctggc cgcagctggt
    30481 cgcaggtggg ctatacccca aatttggtca ctattgcctg atgccactgg cggcggaagg
    30541 gcatattttt ggtggctgtg aatttattcg ttatgacgat cgtccctgga gcgaaaaaga
    30601 gttcaatcgt ctgcaaacat ttacgcagat cgtttctgtc gtcaccgaac aaatccagag
    30661 ccgcgtcgtc aacaatgtcg actatgagtt gttatgccgg gagcgcgata acttccgcat
    30721 cctggttgcc attaccaacg cggtgctttc ccgcctggat atggacgaac tggtcagcga
    30781 agtcgccaaa gaaatccatt actatttcga cattgacgat atcagtatcg tcttacgcag
    30841 ccaccgtaaa aacaaactca acatctactc cactcactat cttgataaac aacatcccgc
    30901 ccacgaacag agcgaagtcg atgaagccgg aaccctcacc gaacgcgtgt tcaaaagtaa
    30961 agagatgctg ctgatcaatc tccacgagcg ggacgattta gccccctatg aacgcatgtt
    31021 gttcgacacc tagggcaacc agattcaaac cttgtgcctg ttaccgctga tgtctggcga
    31081 caccatgctg ggcgtgctga aactggcgca atgcgaagag aaagtgttta ccactaccaa
    31141 tctgaatttg ctgcgccaga ttgccgaacg tgtggcaatc gctgtcgata acgccctcgc
    31201 ctatcaggaa atccatcgtc tgaaagaacg gctggttgat gaaaacctcg ccctgaccga
    31261 gcagctcaac aatgttgata gtgaatttgg cgagattatt ggccgcagcg aagccatgta
    31321 cagcgtgctt aaacaagttg aaatggtggc gcaaagtgac agtaccgtgc tgatcctcgg
    31381 tgaaactggc acgggtaaag agctgattgc ccgcgcgatc cataatctca gtgggcgtaa
    31441 taatcgccgc atggtcaaaa tgaactgcgc ggcgatgcct gccggattgc tggaaagcga
    31501 tctgtttggt catgagcgtg gggcttttac cggtgccagc gcccagcgca ttggtcgttt
    31561 tgaactggcg gataaaagct ctctgttcct cgacgaagtg ggcgatatgc cactggagtt
    31621 acagccgaag ttgctgcgtg tattgcagga acaggagttt gaacgtctcg gcagcaacaa
    31681 aatcattcag acggacgtac gtttaattgc cgcgactaac cgcgatctga aaaaaatggt
    31741 cgccgaccga gagttccata gcgatctcta ttaccgcctg aacgtattcc cgattcacct
    31801 gccgccacta cgcgagcgtc cggaagatat tccgctgctg gcgaaagcct ttaccttcaa
    31861 aattgcccgt cgtctggggc gcaatatcga cagcattcct gccgagacgc tgcgcacctt
    31921 gagcaacatg gagtggccgg gtaacgtacg cgaactggaa aacgtcattg agcgcgcggt
    31981 attgctaaca cgcggtaacg tgctgcagct gtcattgcca gatattgttt taccggaacc
    32041 tgaaacgccg cctgccgcaa cggttgtcgc ccaggagggc gaagatgaat atcagttgat
    32101 tgtgcgcgtg ctgaaagaaa ccaacggcgt ggttgccggg cctaaaggcg ctgcgcaacg
    32161 tctggggctg aaacgcacga ccctgctgtc acggatgaag cggctgggaa ttgataaatc
    32221 ggcattgatt taactgcaaa ttgccggaca gatctgcctg tccggcatac tattcacgag
    32281 gttttttcgg acgatatttt tccggcagtt ctggtaccgg acgcttgtca tcgatgagat
    32341 gacgcacggt taagatcgga tgacgccaca gcattctcgg cccggcccaa cgcataatct
    32401 gtttcatctc ttcacgcttt gcaggctggt aacagtgcac cggacactgc ttacaggctg
    32461 gtttctcttc gccgaacaca catttatcca gccgcttttg cgcgtaaaca aacaacgcct
    32521 cgtaatgctc cggctccgct gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt
    32581 ttttaatcgt cagtttttca cgagagatac gcttgccgga catgctgcct ccatactctt
    32641 aaggtgtatt tatattacaa cttaatttga aaggggacta tggctctgaa ggaggaggtt
    32701 tattcaaccg acgcaatttt gcgtacttgc ttcataagca tcacgcaaaa gctgcaaaac
    32761 agcatctttc ccggaaccag catcaagaac tcgccgttcg cttcttcccc tgaaatgatt
    32821 aactccggta tcatgtgcgc cttatgtgat tacaacgaaa ataaaaacca tcacacccca
    32881 tttaatatca gggaaccgga cataacccca tgagtgcaat agaaaatttc gacgcccata
    32941 cgcccatgat gcagcagtat ctcaggctga aagcccagca tcccgagatc ctgctgtttt
    33001 accggatggg tgatttttat gaactgtttt atgacgacgc aaaacgcgcg tcgcaactgc
    33061 tggatatttc actgaccaaa cgcggtgctt cggcgggaga gccgatcccg atggcgggga
    33121 ttccctacca tgcggtggaa aactatctcg ccaaactggt gaatcaggga gagtccgttg
    33181 ccatctgcga acaaattggc gatccggcga ccagcaaagg tccggttgag cgcaaagttg
    33241 tgcgtatcgt tacgccaggc accatcagcg atgaagccct gttacaggag cgtcaggaca
    33301 acctgctggc ggctatctgg caggacagca aaggtttcgg ctacgcgacg ctggatatca
    33361 gctccgggcg ttttcgcctg agcgaaccgg ccgaccgcga aacgatggcg gcagagctgc
    33421 aacgcactaa tccggcggaa ctgctgtatg cagaagattt cgccgagatg tcgctgattg
    33481 aaggccgtcg cggcctgcgc cgtcgcccgc tgtgggagtt tgaaatcgac accgcgcgcc
    33541 agcagttgaa tctgcaattt ggcacccgcg atctggtcgg ttttggcgtc gagaacgcgc
    33601 cgcgcggact ttgtgctgcc ggttgtctgt tgcagtatgc gaaagatacc caacgcacga
    33661 ccctgccgca tattcgttct atcaccatgg aacgtgagca ggacagcatc attatggatg
    33721 ccgcgacacg tcgtaacctg gaaattactc agaacctggc gggcggtgcg gaaaatacgc
    33781 tggcttctgt gctcgactgc accgtcacgc cgatgggtag tcgtatgctg aaacgctggc
    33841 tgcatatgcc agtgcgcgat acccgcgtgt tgcttgagcg ccagcaaact attggcgcat
    33901 tgcaggattt caccgccgag ttgcagccgg tactgcgtca ggtcggcgac ctggaacgta
    33961 ttctggcacg tctggcttta cgaactgctc gcccacgcga tctggcccgt atgcgccacg
    34021 ctttccagca actgccggag ctgcgtgcgc agttagaaac tgtcgatagt gcaccggtac
    34081 aggcgctacg tgagaagatg ggcgagtttg ccgagctgcg cgatctgctg gagcgagcaa
    34141 tcatcgacac accgccggtg ctggtacgcg acggtggtgt tatcgcatcg ggctataacg
    34201 aagagctgga tgagtggcgc gcgctggctg acggcgcgac cgattatctg gagcgtctgg
    34261 aagtccgcga gcgtgaacgt accggcctgg acacgctgaa agttggcttt aatgcggtgc
    34321 acggctacta cattcaaatc agccgtgggc aaagccatct ggcacccatc aactacatgc
    34381 gtcgccagac gctgaaaaac gccgagcgct acatcattcc agagctaaaa gagtacgaag
    34441 ataaagttct cacctcaaaa ggcaaagcac tggcactgga aaaacagctt tatgaagagt
    34501 tgttcgacct gctgttgccg catctggaag cgttgcaaca gagcgcgagc gcgctggcgg
    34561 aactcgacgt gctggtgaac ctggcggaac gggcctatac cctgaactac acctgcccga
    34621 ccttcattga taaaccgggc attcgcatta ccgaaggtcg ccatccggta gttgaacaag
    34681 tactgaatga gccatttatc gctaacccgc tgaatctgtc gccgcagcgc cgtatgttga
    34741 tcatcaccgg tccgaacatg ggcggtaaaa gtacctatat gcgccagacc gcactgattg
    34801 cgctgatggc ctacatcggc agctatgtac cggcacaaaa agtcgagatt ggacctatcg
    34861 atcgcatctt tacccgcgta ggcgcggcag atgacctggc ttccgggcgc tcaaccttta
    34921 tggtggagat gactgaaacc gccaatattt tacataacgc caccgaatac agtctggtgt
    34981 taatggatga gatcgggcgt ggaacgtcca cctacgatgg tctgtcgctg gcgtgggcgt
    35041 gcgcagaaaa tctggcgaat aagattaagg cattgacgct gtttgccacc cactatttcg
    35101 agctgaccca gctaccggag aaaatggaag gcgtcgccaa cgtacatctc gatgcgctgg
    35161 agcacggcga cactatcgcc tttatgcaca gcgtgcagga cggcgcggcg agcaaaagct
    35221 acggtctggc ggttgctgct ctggcgggcg taccgaaaga ggttattaag cgcgcacggc
    35281 aaaaactgcg tgagctggaa agcatttcgc cgaacgccgc cgcgacgcaa gtggatggta
    35341 cgcagatgtc tttgctgtca gtgccagaag aaacttcgcc tgcggtcgaa gcactggaaa
    35401 atctcgaccc ggattcactg actccgcgtc aggcgctgga gtggatttat cgcttgaaga
    35461 gtctggtgta ataacaattc ccgatagtct tttgctatcg ggaatattaa cgacaactga
    35521 cgaatcaaat aaaaataccc tgtataatag gaaagcttat tttacagggt aaaaccatgc
    35581 catctacacg ctatcaaaaa atcaatgccc atcactatcg ccatatatgg gtcgttggtg
    35641 atattcatgg tgaatatcag ttattacaat cccgttaaca tcaactctct ttttaccctg
    35701 aaaccgactt acttatttcc acaggcgata atattgatcg tgggccgaag agtcttaacg
    35761 tgctgcgcct gctaaaccaa ccctggttta cgtcggttaa aggcaaccac gaagcgatgg
    35821 cgttggatgc attcgaaact ggcgatggaa atatgtggct tgccagcggt ggtgactggt
    35881 ttttcgattt aaatgattca gagcaacaag aggcaataga tctgttgctg aaattccatc
    35941 accttccaca tattattgaa atcattaatg acaacataaa atatgccatc
//
LOCUS       DAZOYS010000022        34583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOYS010000022 DAZOYS010000000
VERSION     DAZOYS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.022"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            231..383
                     /gene="mokC"
                     /locus_tag="ACHUN9_000937"
     CDS             231..383
                     /gene="mokC"
                     /locus_tag="ACHUN9_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0694417.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(487..1617)
                     /gene="dnaJ"
                     /locus_tag="ACHUN9_000938"
     CDS             complement(487..1617)
                     /gene="dnaJ"
                     /locus_tag="ACHUN9_000938"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0694418.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1706..3622)
                     /gene="dnaK"
                     /locus_tag="ACHUN9_000939"
     CDS             complement(1706..3622)
                     /gene="dnaK"
                     /locus_tag="ACHUN9_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0694419.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3999..4403
                     /locus_tag="ACHUN9_000940"
     CDS             3999..4403
                     /locus_tag="ACHUN9_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0694420.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4429..5142
                     /gene="msyB"
                     /locus_tag="ACHUN9_000941"
     CDS             4429..5142
                     /gene="msyB"
                     /locus_tag="ACHUN9_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0694421.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5291..5857
                     /gene="satP"
                     /locus_tag="ACHUN9_000942"
     CDS             5291..5857
                     /gene="satP"
                     /locus_tag="ACHUN9_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0694422.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5892..6479)
                     /gene="mog"
                     /locus_tag="ACHUN9_000943"
     CDS             complement(5892..6479)
                     /gene="mog"
                     /locus_tag="ACHUN9_000943"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0694423.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6594..7547)
                     /gene="tal"
                     /locus_tag="ACHUN9_000944"
     CDS             complement(6594..7547)
                     /gene="tal"
                     /locus_tag="ACHUN9_000944"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0694424.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7826..9255
                     /locus_tag="ACHUN9_000945"
                     /pseudo
     CDS             7826..9255
                     /locus_tag="ACHUN9_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9325..10101
                     /gene="yaaA"
                     /locus_tag="ACHUN9_000946"
     CDS             9325..10101
                     /gene="yaaA"
                     /locus_tag="ACHUN9_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0694425.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10254..10550)
                     /locus_tag="ACHUN9_000947"
     CDS             complement(10254..10550)
                     /locus_tag="ACHUN9_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0694426.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10764..12050)
                     /gene="thrC"
                     /locus_tag="ACHUN9_000948"
     CDS             complement(10764..12050)
                     /gene="thrC"
                     /locus_tag="ACHUN9_000948"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0694427.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12051..12983)
                     /gene="thrB"
                     /locus_tag="ACHUN9_000949"
     CDS             complement(12051..12983)
                     /gene="thrB"
                     /locus_tag="ACHUN9_000949"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0694428.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12985..15447)
                     /gene="thrA"
                     /locus_tag="ACHUN9_000950"
     CDS             complement(12985..15447)
                     /gene="thrA"
                     /locus_tag="ACHUN9_000950"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0694429.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKTRDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15474..15593)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15529..15594)
                     /gene="thrL"
                     /locus_tag="ACHUN9_000951"
     CDS             complement(15529..15594)
                     /gene="thrL"
                     /locus_tag="ACHUN9_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0694430.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15808..16494)
                     /locus_tag="ACHUN9_000952"
     CDS             complement(15808..16494)
                     /locus_tag="ACHUN9_000952"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0694431.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16894..17034)
                     /gene="yjjY"
                     /locus_tag="ACHUN9_000953"
     CDS             complement(16894..17034)
                     /gene="yjjY"
                     /locus_tag="ACHUN9_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0694432.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17130..17846
                     /gene="arcA"
                     /locus_tag="ACHUN9_000954"
     CDS             17130..17846
                     /gene="arcA"
                     /locus_tag="ACHUN9_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0694433.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17906..19258)
                     /gene="creD"
                     /locus_tag="ACHUN9_000955"
     CDS             complement(17906..19258)
                     /gene="creD"
                     /locus_tag="ACHUN9_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0694434.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19316..20740)
                     /gene="creC"
                     /locus_tag="ACHUN9_000956"
     CDS             complement(19316..20740)
                     /gene="creC"
                     /locus_tag="ACHUN9_000956"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0694435.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20740..21429)
                     /gene="creB"
                     /locus_tag="ACHUN9_000957"
     CDS             complement(20740..21429)
                     /gene="creB"
                     /locus_tag="ACHUN9_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0694436.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21442..21915)
                     /gene="creA"
                     /locus_tag="ACHUN9_000958"
     CDS             complement(21442..21915)
                     /gene="creA"
                     /locus_tag="ACHUN9_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0694437.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22126..22995
                     /gene="robA"
                     /locus_tag="ACHUN9_000959"
     CDS             22126..22995
                     /gene="robA"
                     /locus_tag="ACHUN9_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0694438.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22992..23639)
                     /gene="gpmB"
                     /locus_tag="ACHUN9_000960"
     CDS             complement(22992..23639)
                     /gene="gpmB"
                     /locus_tag="ACHUN9_000960"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0694439.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23691..24212
                     /gene="yjjX"
                     /locus_tag="ACHUN9_000961"
     CDS             23691..24212
                     /gene="yjjX"
                     /locus_tag="ACHUN9_000961"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0694440.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24297..24623)
                     /gene="trpR"
                     /locus_tag="ACHUN9_000962"
     CDS             complement(24297..24623)
                     /gene="trpR"
                     /locus_tag="ACHUN9_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0694441.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24713..26650)
                     /gene="sltY"
                     /locus_tag="ACHUN9_000963"
     CDS             complement(24713..26650)
                     /gene="sltY"
                     /locus_tag="ACHUN9_000963"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0694442.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26861..28528
                     /gene="ettA"
                     /locus_tag="ACHUN9_000964"
     CDS             26861..28528
                     /gene="ettA"
                     /locus_tag="ACHUN9_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0694443.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28689..28796)
                     /gene="nadS"
                     /locus_tag="ACHUN9_000965"
                     /pseudo
     CDS             complement(28689..28796)
                     /gene="nadS"
                     /locus_tag="ACHUN9_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(28835..30067)
                     /gene="nadR"
                     /locus_tag="ACHUN9_000966"
     CDS             complement(28835..30067)
                     /gene="nadR"
                     /locus_tag="ACHUN9_000966"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0694444.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30088..31470)
                     /gene="radA"
                     /locus_tag="ACHUN9_000967"
     CDS             complement(30088..31470)
                     /gene="radA"
                     /locus_tag="ACHUN9_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0694445.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31519..32487)
                     /gene="serB"
                     /locus_tag="ACHUN9_000968"
     CDS             complement(31519..32487)
                     /gene="serB"
                     /locus_tag="ACHUN9_000968"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0694446.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32593..33237
                     /locus_tag="ACHUN9_000969"
     CDS             32593..33237
                     /locus_tag="ACHUN9_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0694447.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33265..34281
                     /gene="lplA"
                     /locus_tag="ACHUN9_000970"
     CDS             33265..34281
                     /gene="lplA"
                     /locus_tag="ACHUN9_000970"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0694448.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34313..34576
                     /locus_tag="ACHUN9_000971"
                     /pseudo
     CDS             34313..34576
                     /locus_tag="ACHUN9_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8166 a         9397 c         8624 g         8396 t
ORIGIN      
        1 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaga atgggttaaa
       61 gagagcgata taatagcggc gggtgcttga ggctatctgt ctcaggcatt agctgaacgg
      121 cagatagaga aaagccccga gtgatatttt actatcaacc cgaggccccc tatatgctga
      181 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
      241 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
      301 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
      361 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc acttttcagg
      421 tgctcgcata tcttcaacgc accctaattt tacccaagcc tgcccacggg caggcttttg
      481 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
      541 gcgcgggctg ttgtgctcac cggttgggcc accgaagctt tcttgcagct cttgcagcaa
      601 ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
      661 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga aaagcttacc
      721 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
      781 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataga ggttgttgcc
      841 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
      901 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
      961 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     1021 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     1081 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     1141 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     1201 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     1261 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     1321 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac cgccgccgcc
     1381 catgccacct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     1441 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     1501 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttttgatttc
     1561 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     1621 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     1681 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     1741 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     1801 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     1861 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     1921 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     1981 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     2041 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     2101 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     2161 cggaaacgtg caagataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     2221 cgcgcggtgc cgggttgata ccatccaggt tgaactgacc cagagatttg ttatcagctg
     2281 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     2341 tagagaacac ctggctgtgc ttggtcggga tagtggtatt tttcgcgatc agcgtcgtca
     2401 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     2461 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     2521 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     2581 ttggcatacg agtctgacca cccacgagga taacgtcgtc gatatcagat acggacaggc
     2641 cagcgtcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     2701 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcatctg
     2761 cagtgatgta cggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     2821 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     2881 gatctttctt gaattcttca accaggtagt tgatcagacg gctgtcgaag tcttcacccc
     2941 ccaggtgggt atcaccgttg gtagccagaa cttcgaaggt tttttcgccg tcaacttcgt
     3001 cgatttcgat aatagaaata tcgaaagtac caccacccag gtcataaacc gcgatagtac
     3061 ggttgccagt acctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     3121 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     3181 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aagtaatctt
     3241 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     3301 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     3361 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     3421 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     3481 tttcaccatc ctgggtatag gcaataatag aaggcgtggt gcgatcgcct tcggcgttct
     3541 ccagcacgcg aggagtggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     3601 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     3661 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     3721 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     3781 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     3841 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcattca tcaaaaaatt
     3901 gtaataaaaa gaaaagatta cgtacctgaa tcttctcttt atcagcagta aactagtggg
     3961 tattcatccc tctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     4021 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     4081 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     4141 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagcg
     4201 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagtctgaat attccttcag
     4261 aaataaaaga agggcaaacc actgactgga tcaacattaa cagtgataac gacaataaac
     4321 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     4381 tgaaaattat cggcgacgac taacgccaga atatagaaac cacaaaaaat gaatgttaat
     4441 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     4501 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg
     4561 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     4621 ctgattgctg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     4681 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa aacgattgaa gctgaaagcc
     4741 gacaaagaga tgtctacgtt tgagattgag caacagttac tggaacaatt tctgcgtaac
     4801 acctggaaga atatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     4861 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggc
     4921 gtgtcgcatt tgctttccag ccaattgacc cgcattttac gcacccacgc agcaatgagc
     4981 gtgcttgggc atggtttgct gcgcggcgcg ggtctgggag gccctgtggg cgcagcatta
     5041 aatggggtta aagcggtcag cggcagtgcc tatcgcgtga caattccagc cgtaatacaa
     5101 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gacaccgtaa accggggaaa
     5161 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     5221 gccctgaaaa cactactgtt attcagggaa attatttcac cattcattcg atgatgattt
     5281 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     5341 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     5401 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgccg gtctgctgga
     5461 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggtt
     5521 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     5581 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     5641 gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc ctgactgtgc tgtttgccct
     5701 gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc cactttgccg gctggattgg
     5761 tctgatctgt ggtgccagtg caatctatct ggcgatgggt gaagtactga acgagcagtt
     5821 tggtcgcacc gttctgccga ttggtgaatc ccactaattg ttttgagatc cccccgctcg
     5881 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     5941 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     6001 gcaaaaatac cgtgtaccac aacgttaccc gcagcgtcct tcacaccttc cagcgtctct
     6061 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacacca
     6121 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc ttatctggcg catctgttca
     6181 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     6241 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccactagc
     6301 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     6361 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg cagggatgcc tttatcctga
     6421 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     6481 aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca
     6541 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     6601 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     6661 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     6721 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     6781 tcgctctcag ccagctcttt cagcagtgcc ggtgcgatgg taagacggtc gcagcctgcc
     6841 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     6901 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     6961 gcgtactctt tcttatcagt attcgctttg taccagtcaa gaatacggcc aacaaatggc
     7021 gagatcagga acacgcctgc ttccgcacaa gcacgagcct gagcgaagga gaacagcagg
     7081 gtcaggttac agttgatgcc ttctttttcc agctgttctg cagcacggat accctgccag
     7141 gtagaagcca gtttgatcag aatacgatcg ttgctaatac cagcatcgtt gtagagtttg
     7201 atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact
     7261 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
     7321 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
     7381 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
     7441 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
     7501 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
     7561 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
     7621 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
     7681 gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca
     7741 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
     7801 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
     7861 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
     7921 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
     7981 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
     8041 gggtagcggc aacctggccg gcgttgcgct ggctattacc gccggtggac ctggagccgt
     8101 cttttggatg tgggttgccg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
     8161 tgcacaactt tataaagaac gtgacgtcaa tggacagttt cgtggaggac cggcatggta
     8221 tatggcgcgc gggctgggga tgcgttggat gggcgttctg ttcgccgtct ttttgctcat
     8281 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
     8341 tcttttgatt ttcccccgct ggtgacaggc attattctcg ctgtctttgc tctgctggca
     8401 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg
     8461 ataatctggg tgctgaccag catggtgatt tgcgtaataa atatcgggca acttccccac
     8521 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg
     8581 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
     8641 gcgggaatgg gttcgacgcc aaacgcggca gcggcagcgg cgtcctggcc tccgcatccg
     8701 gcagcgcaag ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg
     8761 gcaagcgcca tgctgatatt actggcgggt aacgacacaa cctacatgcc gctggaaggt
     8821 attcagctta tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc tgagtttgtt
     8881 accctcgtgg ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa
     8941 aacaatctct tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc
     9001 accttcgcaa cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg gcaactggca
     9061 gatatcataa tggcctgcat ggctattacc aatttgaccg ccattttact gctctcgcct
     9121 gtggttcata ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg
     9181 tttgatccgt tgcgttatcc ggatatcggt cgccagcttt ctccggacgc gtgggatgat
     9241 gtttcgcagg agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc
     9301 ataaatttcc tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat
     9361 taccaaagcc cgttgaccac cacgcgctat acgctgccgg agctgttaga caattcccag
     9421 cacttgatcc atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgcatc
     9481 agcgacaaac tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcacg
     9541 ccggagaatg cccgccaggc tattttggcg tttaaaggtg atgtctacac cggcttgcag
     9601 gccgaaactt tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc
     9661 ggcttgtatg gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg
     9721 atccgtcttg agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc
     9781 aacaagctga acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca
     9841 gatgagtatt ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg
     9901 ttcctcgatg agaagaacgg caaatttaag ataatcagct tctacgctaa gaaagcacgc
     9961 ggtctgatga gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt
    10021 tttaatagcg aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt
    10081 aaacgctacg agcagcgtta agtttgcagt caatgccgga tgcggcgtga acgccttatc
    10141 cggcctacaa aagcatgcag attcaatata ttgcagaatt cacgtaggcc tgataagacg
    10201 cgccagcgtc gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg
    10261 tgatgtttgc ctggaccatg accgccgtga tgatcatgag gagctttctt atggtggcgc
    10321 ggcggtggcg gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc
    10381 caccagccgt ggtcgcgcca gtgacctcca tcccagtaat agccacgatt atcacgatcg
    10441 cctatctgta attttactga cgggactaac gtaatttccg cagcctgtgc tgccatggga
    10501 gcgaccagaa ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc
    10561 ttttatcatt gcccgttatg ggtcgatatc cggagattac gcgatgagaa cgacggttgt
    10621 tattctccgc aatcctaatc tctaagttac cgcatttatt gagaatttct cctttttccc
    10681 tgtcgttttt ctccataatt tcttcataaa aaagccgggc tgcataaaag caaacccggc
    10741 ctgattgaga taatgaatag attttactga tgattcatca tcaatttacg caacgcagca
    10801 aaatcggcgg gcagattatg tgaaagcaag ggtaaatcag cacgttctgc cagctctttt
    10861 ggcagatcca acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc
    10921 gcggtgccga ggaacaagcc atattcgcct ggattcaact ggtcacacag cgcacgatac
    10981 gctacggcag cgtgcggctc cgaggtgtag cccagttctt ttaactcacg cattgtctgt
    11041 tgcgtggttt catcatccac ggctgcataa cccagctctt tcagttgcca gattttgcgg
    11101 cggaacaact cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat
    11161 aacgtcgcct gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta
    11221 tcgttcacgt tggtcgcagc aataaaacgt ttcaccggca gaccgagtga cttcgccagc
    11281 agacccgccg tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc
    11341 gcttcctgcg gcagctgcgc aactgcttca aagtagtagc aaatctgcgc cagcaaacgg
    11401 ctgatgttaa tcgagttagc cgagtttaac cccagcgcca ctttcagttc ttcatcatca
    11461 aacgcctgct tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg
    11521 atattgccgc ccaacgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga
    11581 tagaggataa ccactttcac attcggcaaa ccgtagaaag catgagccac tgccgctccg
    11641 gtatcaccgg aggtcgcggt cagaatggtc actggcttat cgcccgcaat atgggtcagc
    11701 atttgtgcca taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat
    11761 tccagacaac cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg
    11821 cgcacgcgct cttccaggat ttcctgcggg atttcatcac caataaacgc cgagaggatc
    11881 ttcgcactgc gggtgacaaa atccagcttc agcatctcat caatttcagt caggctgaat
    11941 tccggcaggt catgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct
    12001 tgcgcaaagc tgacctgctc gttgtgatct ttcagattgt agagtttcat ttagttttcc
    12061 agtactcgtg cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc
    12121 aggtagttct tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacataga
    12181 gcgaacaagg tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc
    12241 gcctgccgcg cctgccggaa gccaggcagt aaccgttcac ggtagggttc agcgataaca
    12301 tctttcatca gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcca
    12361 gccagatgtc gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc
    12421 cgggcttctg ccgtcgagac tttaattccc ggatacgcca gcacccacag ccactcatca
    12481 aaccctggca cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca
    12541 agaaaacacg gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc ttccagctcg
    12601 cccatcaaag ccagcaaacg ggtgtcatta agtggcttgc cgcagtgttc attcatcgcc
    12661 atcagcgccg cgaccaccga acaggcgctg gagcctaagc ccgagccgat cggcatattc
    12721 ttttccagag tcatcgccac tggaatttgc ttgcccagct ccaggcaaaa acgctcccag
    12781 cactgataaa cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagg
    12841 ttgttgagac tgaatgtctc tgccgcctca accgtgacta catctccgag caatgcacca
    12901 tcaacgggtg tcaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa
    12961 gccggggcat aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag
    13021 atcggcaaag acaccagcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa
    13081 cggcagcggc tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt
    13141 gaacagcgga tcattcccat ccacttcggc aatcttcacg cggcagacac catcttcatc
    13201 aatattgcca acatagcgca aaacttttcc ttcatcacgg gtcttcgcca cgcgcgcggc
    13261 aaagagatcg tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcggcgtt
    13321 aaactctgcg ggcagcacag gttcaatttc aatatccgcc agctccagtt cacgtcccgt
    13381 ttcacgagcg agaatcaata gtttacgcgc cacatccata ccagaaagat catctcgcgg
    13441 gtccggttcg gtataaccca tttcccgcgc cagcgtggtc gcctcggaga aactcatgcc
    13501 ttcgtctaac ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcac
    13561 caattcatca cctgcattga gcagattttg caggttctca ataaccggta atccagcccc
    13621 aacgttggtg tcatagagga atttacgccg cgatttttcc gccgcataac gcaactgatg
    13681 atagtaatcc atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc
    13741 gcgcaggaag tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac
    13801 cgggttcagc agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc
    13861 tttggcttgt gcaagttctt cctgccagtt ttccagatta aggccatgca cactagtgag
    13921 cagagccttc gagttggcaa cgccgcagac acgtaagtcg atatgtttat tcttcagcca
    13981 gctttgctga cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac
    14041 aaacacttcg ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt
    14101 ggtcgcatca tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat
    14161 ggcgacaatg ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg
    14221 caaggtgcgc ataccatcac ctaccaccga gataatggcc agccgttcca tcaccgccag
    14281 cggctccagt aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc
    14341 ccgcacacag tcgctttgcg gaacgcagaa actgatactg tattcggaag atgattgcgt
    14401 aatcagcacc acggaaatac gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc
    14461 gaccattcct ttcatccccg ggccggaaac gctgaacatt gccatgttat tcagattgga
    14521 aatgcccttg accggtaatt cgccttcatc acgactggca ccaatgagcg tacctggtgc
    14581 ttgaggattt ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat
    14641 ggtgcggggg tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga
    14701 catcgacttc aacaacctcg catcgggcac ctgacgcggg tcgcaggtat agaccccgtc
    14761 aacgtccgtc caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga
    14821 gtagtcggaa ccgttgcgtc caagtaccac cagttcgcct ttttcattac cggcggtgaa
    14881 gcctgccatc agcaccatgt gatcagccgg aatacgactt gccgcaatac ggcgggtgga
    14941 ctcagcaata tcgacggtag attcgaggta atgccccact gccagtagtt tttcgaccgg
    15001 atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa tggcgatcga
    15061 cattttctcg ccacggcaaa tcagcgcagc gttaatgcta tccgggcact gccctaacaa
    15121 actaatgcca tgcaggacat gttttatttg ggcaaattcc tggtcgacga aagttttcaa
    15181 ttgcgccagc gggaatcccg gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat
    15241 acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat
    15301 cgccaccagg tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg
    15361 cctggcattg ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat ttgccactga
    15421 tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa
    15481 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    15541 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    15601 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    15661 tgacctaagt caataaaatt ttaatttact catggcaggt aaccagttca gaagctgcta
    15721 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    15781 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    15841 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    15901 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    15961 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    16021 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    16081 atccgccacc atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag
    16141 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    16201 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactgcgcgc
    16261 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgttg ggaaaacttt
    16321 aatattatca ataacatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    16381 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    16441 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    16501 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    16561 aatttacaaa ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct
    16621 aaactatttc ctgactgtac taacggttta attgttaaaa aatgctacat atccttctgt
    16681 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    16741 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    16801 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    16861 agttttattg ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat
    16921 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    16981 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    17041 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    17101 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    17161 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    17221 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    17281 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    17341 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    17401 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    17461 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    17521 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    17581 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    17641 acttctgtga aaacccaggc aaaattcagt cccgtgctga actgctgaag aaaatgaccg
    17701 gccgtgagct gaaaccacac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    17761 atttcgaatc tacgccggat acgccggaaa tcatcgccac catccacggt gaaggttatc
    17821 gcttctgtgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt
    17881 ttagcgccgt ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa
    17941 cttctttact ggctttcatt ttcggcagtg aaaacgcata ccagtcgata ttacgagtca
    18001 caaacatcat gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat
    18061 cgacagagtt gagcagtccc cacatcactc catccagcaa caacagcgcg agggtaaaca
    18121 acatgctatt gcgccagcct ttcaataccg cctgcaaata aataccgttc attaacgccc
    18181 caatcagact ggcgattatc catgccacgg taaaaccgat atgttcagaa agcgccagca
    18241 agagcaaata aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac
    18301 gttgcgcggt gagcgtttca aaaacaaaga acgccataaa agtcagtgca atcagcagaa
    18361 tggcgtactt agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc gtcactgcga
    18421 cgctaaacgc gaggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga
    18481 gattattagc aaaccagctg ctttgccatt gcgcctgaaa acctgactcg ctaacttccc
    18541 gtttggctgg caaaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca
    18601 tttcgctatt acgcccggca ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg
    18661 ccatattcag cttcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc
    18721 cctgcccgcc ttgctctaac ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt
    18781 caggcgcttt caccacacca ataccacgcg catccccgac gctaatcaca ataaatggct
    18841 tgccaaaggt gatatttggc gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt
    18901 ttagcgttaa atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca
    18961 cgttctgatt gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt
    19021 tccgctccac ggttttatcc tcttcctgta ccgtataaag ctcggtcaca ggaatagcga
    19081 taagcggccc aacaagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat
    19141 cgctacggta atcggcacgt tcgacaatca cctgccgaat cagcattatc ggaatcaaca
    19201 acagcaatac tgcaccaaac agggtggtca ttttccagaa cagggaggat ttcaacatag
    19261 tcttctcctt tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg
    19321 tgaagtgacg gtgaagtcga agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg
    19381 tgacttcgcc gttaaacaaa cgggcgacct cactgacgaa cgccaacccc agaccgctgc
    19441 ttttttgccc atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt
    19501 agtcaggaat accactaccg gtatccagca ccttaagcgt gacgtgttcc tgatccactt
    19561 cggcgcttag cgttatacga ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat
    19621 tccccagcgc ctgatccagt aacgccggtt cagcagcaac attaacctcg gtgggcgtaa
    19681 catgtagagt gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgga
    19741 ataatgccgc cacatcaaca gcagtcaaaa cgacttcctg acgattctcc agtcttgcct
    19801 ggcgtagtaa cgtttccacc agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcag
    19861 taaaacgagc cactacttca ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa
    19921 tcgccgccag tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt
    19981 ttttcccttc cagcttcacg cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc
    20041 tactaccgag atcgggaaga ggaacgggct tattgtcagt gacggaatca gcatagcgag
    20101 tgagccgggc aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcga
    20161 tccccaacaa aatggcgctg gcccataaaa ttcgccgctc gctgcgttta atgaccggag
    20221 ccatcgccgc gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgtccataa
    20281 tcggcgcggc aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctac
    20341 gcgcaccata ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc
    20401 caacggcttt atttgccgaa tcgaacaata ctttgccctg cgcatcggtc atatagacat
    20461 gatattcatt gcgcactttg ttaatgccac cgatattggc gcgaaacggg cgatgttgta
    20521 gctgattaaa cgcctgcgcc agttgcccat gcgttgggtc cccagagagc aaatcgggac
    20581 gcgccagctc cgccagcaac gttgcggtat cgattaacgt cccctccgtt gctcttcgca
    20641 cgcccggttt aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca
    20701 gtaaaaaata gcccagcagc aaccgcatgc cgatacgcat tacaggcccc tcaggctata
    20761 tcccatgccg cgatgagtat taatcggaga aagatcgggg ttgatggcgc gcagcttggc
    20821 acgcaacgtt ttaatgtggg tatcgacggt gcgatcgtag gtgtcctgcg catcttccca
    20881 cacgctatcc attagttgct ggcgggacca gacgcggcct ggcgacttga gtaacgtctt
    20941 cagcaataaa aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc
    21001 cgcgggttca ttcaattcaa aatgaccaat acggatgacg ggagacggcg acgagaactt
    21061 cttcacccga cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt
    21121 agccacatag tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg
    21181 ggccgtcagg aacagtacag gtaacgccgg atggagcgcc agtaactggc ggcacaattc
    21241 aaagccgctg atatcaggca aaccaacatc gagaatcatg acgtcgggaa cctgctggcg
    21301 agctttatcc agcaccggca agcctcgctc aaagacctcg acggcaaaac cttcctgctg
    21361 caacatgtag accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc
    21421 ccgttgcata aatcgcctct gttattgccg ccacggcatg acaggaaccg cgctaatcgc
    21481 gtttttcggc gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg
    21541 tttggcgtca taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt
    21601 gaatactacc tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc
    21661 gacttgctga caagaaatag ccgcatcgga ggtatcttcc gccagaccca atcccccttt
    21721 aataccacct gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc
    21781 aaaggcttcc acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga
    21841 accaatctct tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa
    21901 atgcttgtat ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg
    21961 gtaaataaca tattgattaa gaacaaaatc tcaatacttt tatttccgtc aagccctaaa
    22021 acatactcta ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa
    22081 ttacctgatg tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc
    22141 attattcgcg accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac
    22201 aatgtagcgg cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc
    22261 actggccatg ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca
    22321 ctacgcctga ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa
    22381 cagacattta cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt
    22441 tctcctgaat ggagcgcctt tggtattcgc ccgccgctac gtctgggtga attcacaatg
    22501 ccagagcaca aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac
    22561 tcctgttcgc tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac
    22621 gattttctcg gcaacgcgcc gaccattccg ccagtgctct acggcctgaa tgaaacgcgt
    22681 ccgagtcagg ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat
    22741 caggcagatg gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg
    22801 atgtttacct atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga
    22861 acgtgcatgc caatgctcaa cctgacgcgc cgtaaaggtc aggatattga gcgatactac
    22921 ccggcagaag acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt
    22981 cctatccgcc gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc
    23041 cctgcggttt caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc
    23101 gaaatagaac agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc
    23161 gtactcacca gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc
    23221 ggtaagtccc ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac
    23281 tcgccttcag gaatacgccc gtcaacggtg ccattgacca gctgccgacg ccagttctct
    23341 tcttcttcgg tcagagaatc gatatgtctt ttttccagca cacccatgtt taattcacgc
    23401 aggcgagaat caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc
    23461 cgggtgcgtc ctaaatcgct actgataata tgcgtaatgc caagctcttt ggcacgggtt
    23521 gccacctgca tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga
    23581 atacgtcgct cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg
    23641 ctttttttcc gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag
    23701 ttatctgtgc gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga
    23761 tcttcggcga aggatcctgc catattgcat ccgttgccgt cgagagcggt gtaccggaac
    23821 agccctttgg cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc
    23881 gtttacttcc agaggctgat ttttgggtgg cgattgaagc tggcatcgat ggtgacagca
    23941 ctttcagttg ggtggtgatt gaaaacacca gccagcgcgg cgaggcgcgt tcggctactt
    24001 tgccgttacc tgcggtgatt ctgcagaaag tgcgtgaagg tgaggcgctt ggtcccgtga
    24061 tgtcgcgtta taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta
    24121 ccgccgggaa actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc
    24181 cgtttcacaa tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg
    24241 gcattcggtg cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa
    24301 tcgcttttca gcaacacctc ttccagccac tggcgcagct ccacgggcgc ggctttcagg
    24361 ctgttagatc cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc
    24421 tgactcattt cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg
    24481 cgctcatctg gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca
    24541 ttcttaagca ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct
    24601 gaatagggtg attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgcc
    24661 tcccccggtt gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac
    24721 gacgtcccca ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa taacggtagt
    24781 aagcgtcata agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct
    24841 cgacaaatgc cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc
    24901 caggtccggc gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa
    24961 catattgcag gtaactggtg ccaatgttga tattcgtttc cggatccagc aattgcccag
    25021 ggctgctata accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa
    25081 tctgcatcag gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct
    25141 gacgggcaat cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga
    25201 aaagatcgtt gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga
    25261 tcgttgcctg aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag
    25321 cctgctctgt ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtgttat
    25381 cgagattcca gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggcgc
    25441 tgtcaacatt ctgcggcgct ttatcaatct tcagctcata ctcttctccg atgcgttgtg
    25501 ctgcaaccat cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt
    25561 cagcttcacg tccgcgttcc agcaataaat ccacctgcca gtaacgccat tcatctttct
    25621 cttttgcttc catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc
    25681 caagtgccat tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatgacgt
    25741 catcgcgcca tttcgcctgc tcgtcggtga catcgttgcc catcaaacgc caggcgacga
    25801 tatcgcgcag ctcctgaatc tgatcttcat taagctgctg cacctgggca agcgacggga
    25861 tcatcagccg tgcattttca gcatcctgcc gcgccacact ggcaaacgcc accgctgcca
    25921 tttgacgggt aaaatcggtc gctccggttg tacgcgcgaa ggtcagtacc gtattagggt
    25981 tgttcgccag tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg
    26041 ccagcactgt taccaggcca gtgttacccg ctttcatcgc cagacggata cgctctaaat
    26101 acgccagcgg atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt
    26161 taggctggct cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct
    26221 gcccggtgtt ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt
    26281 ccgggctaaa ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac
    26341 gagattgcag cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg
    26401 tcaccgccgg ttgattcatc agatcatcgg tgatctggcg gtattccagg tagggataaa
    26461 gcggataatc cttcagtcca ggcatcattt gttcgaccac atccatttgt cgattatccc
    26521 aggcctgctt gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca
    26581 cgctgctgac cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg
    26641 ctttttccac aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa
    26701 tagatttatg ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt
    26761 actgcgaagg tgatcgacca actgggatta ggcggggaaa aaggctacac ttcgcctttg
    26821 aaaactcatc atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac
    26881 catgcatcgt gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct
    26941 gagtttcttc cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtctac
    27001 cctgctgcgc attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc
    27061 agacatcaag attggttacc tgccgcagga accgcagctg aacccggaac acaccgtgcg
    27121 tgagtccatt gaagaagcgg tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt
    27181 gtatgcgctg tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg
    27241 tctggaagag atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc
    27301 ggcggatgcg ctacgtctgc cggactggga cgcgaaaatc gctaacctct ccggtggtga
    27361 gcgtcgtcgc gtagcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga
    27421 cgaaccgacc aaccacctgg atgccgaatc cgtggcctgg ctggaacgct tcctgcacga
    27481 cttcgagggc accgtggtgg cgattaccca cgaccgttac ttcctcgata acgttgcagg
    27541 ctggatcctc gaacttgacc gcggtgaagg tattccgtgg gaaggcaact actcctcctg
    27601 gctggagcag aaagatcagc gcctggcgca ggaagcttca caagaagcgg cgcgtcgtaa
    27661 gtcgatcgag aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg
    27721 taaagcacgt ctggcacgct ttgaagagct gaacagcacc gaatatcaga aacgtaacga
    27781 aaccaacgaa ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag
    27841 caacctgcgt aaatcctacg gtgatcgcct gctgattgat gacctgagct tctcgatccc
    27901 gaaaggggca atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg
    27961 tatgatctct ggtcaggaac agccggacag cggcaccatc actttaggtg aaacggtgaa
    28021 actggcgtcg gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga
    28081 agtttccggc gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta
    28141 cgttggccgc tttaacttta aaggggttga tcagggtaaa cgcgttggtg aactttccgg
    28201 tggtgagcgc ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct
    28261 gctcgacgaa ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct
    28321 gctggagttc ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat
    28381 cgccacgcac atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt
    28441 tactgagtac gaagagtaca agaaacgcac gctgggcgca gacgcactgg agccgaagcg
    28501 tatcaagtac aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gtgaacgcct
    28561 tatccggcct acaaaccaag aaatttcaat acgttgtgaa tctccaggcc tgataagacg
    28621 cggtaagcgt cgcatcaggc attgagcacc gaatgccgga tacggcgtga acgccttttc
    28681 cggcctacaa cagccttagt atccgcaatt tcgtagcggg tcacccgtgc aggttttttc
    28741 aaaccttgtt taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaactta
    28801 ttttatacct cgcctttgag ccgtttcatc gcggttatct ctgctccccc atcatctccc
    28861 gcaccagctc cacgcagcgc aggaaacggc tgtcataatc gtcctcttca acccgcacga
    28921 attcgatatt gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca
    28981 ccgaactgcc gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc tccagcagga
    29041 tcaccagatc gaaacggtat tcatcaatca acgcctgtac gaacggatgc tcacgccctt
    29101 cgtacttttt gcagaacgcc tgggtagtga caaaatcagt gtcaataaac gccactttat
    29161 tggcatattt cactgcaaaa tcaatgtatt gtgcgtggcc cagcgcgatt ttatcgtaat
    29221 cggaatactg caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat
    29281 attcccacgc actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac
    29341 cgctcgactc gccaccaagg atcgccacgg tacgtacaaa gaacggcttc acttccgtag
    29401 gaatatattc ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa
    29461 aggtacgttt cggatcgacc agcaccgtat cgatccccag atgttccata tactgtggcg
    29521 catcggcttc ttccgaggtg tagatcaggt ccggctgaat ccccttttca gccataaact
    29581 ttttgatgcc gttgctccac acatcccagc cgtgcggata cggctccatg ccctcttcgt
    29641 tgaaagcatg aatgcgaata tttttctgat atttaaaagt ttgtaataac cagcgcagac
    29701 gatccggcac cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg
    29761 tatcgtcaaa acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa
    29821 ggtagatatg tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct
    29881 tctgtcgggg aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg
    29941 ggcttttaat tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat
    30001 cagctacctg ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg
    30061 acgacatatc tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga
    30121 gagtttttta acgccaaaaa tctgcatccc ttccggcgct tttttcggta cgttagccgc
    30181 cggaacaatc gcccggcgaa aaccgtgttt cgccgcttct gaaattcgtt cctgaccgct
    30241 gggcaccggg cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgtgg
    30301 cagcggtctg tcacgcaggc tggaaaccat cgccagcagt aacgctaaat cggcactggt
    30361 ttcggttacc ttcacgccgc cgaccacgtt cacaaataca tcctgatcgg ccatttgcag
    30421 accaccgtga cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac
    30481 tgccacgcgg cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa tctccaccag
    30541 caacggacgc gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc
    30601 gcgacttaag aaaattgccg aagggttgct gacttcacgc agcccctgtt cggtcatcgc
    30661 gaagacgccc agctcattca ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa
    30721 acgggagtcg gcatcaccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt
    30781 agggccagcc agcgagccat ctttggttac gtgcccaacc atgacaatcg ccacaccgcg
    30841 cgttttggcg aagcgcgtca gataagccgc cgtttcacgc acctgcgcca cgctaccagg
    30901 cgatgattgt acatccgcca tatgcatcac ctggatcgag tcaattacca tcagtttcgg
    30961 ttgctcttct tcggcaatca ggcagatctg ttcgatgctg gtttccgaca acatattgag
    31021 attgtcagtc ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc
    31081 gccagtgaca tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagtagcag
    31141 cgtggatttc cccgcacctg ggttaccgcc aatcagaatg gcgcttcccg gcaccacgcc
    31201 gccgcctagt acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag
    31261 gctgatatcg gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag
    31321 acgctcgtta cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca
    31381 ggcatgacag gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca
    31441 aacaaaggcg cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc
    31501 aggatggcgg gcgagcaatt acttctgatt caggctgcca gagaggatgc agaatacccc
    31561 catcaggtca gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg
    31621 gtaggcaatc cccagccctg ctgctttgat catcggcagg tcattagctc catcgccaat
    31681 cgccacggtc tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca aagttttcgc
    31741 tttgtactgc gcgtctacga tgtcgccgat cacattgccg gtaaatttac cgtccatgat
    31801 ctccagttca ttggctacca cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa
    31861 gaaagtaaag ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagcac
    31921 cagttgcgtt aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc
    31981 gtcagcgcct ttcagcgtcg ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc
    32041 gcgcatcgcc cgttcggtta cttccgccac catctcgccc gttccggcca gtttggcaat
    32101 ttcatcaata cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg
    32161 cgtgcgcagg tgcgggattt tccccagcgg ggcgacatcc agttgcgctt catgggccag
    32221 gcgcgtagcc cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca
    32281 ccaggcggca acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg
    32341 tttatccagc ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg
    32401 catcacttca tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg
    32461 caggtcgcac caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa
    32521 aataacgcat gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc
    32581 aaagggtgga agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc
    32641 ctgttctgtc ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac
    32701 caccagcgac agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg
    32761 acgctgaacg ttgcaccgct gatgcgtacc gactcaccag atgaaaaacg cattcaggcg
    32821 atcctcgatc agttaacgga tgaaagccgc atcctcgacg cgggtgtgta tgacgaacaa
    32881 ggcgatctta tcgcccgttc aggcgaaagc gtcgaagtgc gcgaccggct ggcgctcgac
    32941 ggtaaaaaag caggcggcta ttttaaccag cagattgtcg agccaattgc gggtaaaaac
    33001 ggaccgctcg gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa
    33061 caggtggata acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc
    33121 gtagtgctga cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc
    33181 ctgttaaccg ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc
    33241 attactacaa gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac
    33301 gacccgtggt ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag
    33361 cgcgttctgt ttctctggcg caatgccgac acggtagtga ttggtcgcgc gcagaacccg
    33421 tggaaagagt gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt
    33481 agcggtggcg gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc
    33541 aagccggagt acgataaaac catctctacg tcgattgtgc tcaatgcgct gaacgcgctc
    33601 ggcgtcagcg ccgaagcgtc cggacgtaac gatctggtgg tgaaaacagt cgaaggcgac
    33661 cgcaaagtct caggctcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcact
    33721 ttgctgctca atgccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa
    33781 ctggcggcga aaggcattac ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg
    33841 ccggggatca cccatgagca ggtttgcgag gccataacca aggccttttt cgcccattat
    33901 ggcgagcgtg tagaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc
    33961 gccgaaacct ttgcccgtca gagtagctgg gaatggaact tcggtcaggc tccggcattc
    34021 tcgcatctgc tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa
    34081 aaaggccata tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa
    34141 gccctcgccg ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc
    34201 gaagcgctgt tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg
    34261 atagcggggg cggtaaggta actcgctgat taaaatacca aaggaaggga atatgatcga
    34321 tctggaaaac caggagcggg agattatcaa tttaatgtta agccagcgta tttcctggct
    34381 agccgccgtg cgtatcaggc ataaattatc gcttgctgaa gtaagtaaaa tgttaggtat
    34441 ctcgattaat tctctaaaat agatcgaaaa gacggaacgg cttagctcaa acatcaaaag
    34501 caaaatggga gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac
    34561 ggctgaacat aaatgaatag att
//
LOCUS       DAZOYS010000023        34132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOYS010000023 DAZOYS010000000
VERSION     DAZOYS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.023"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHUN9_000972"
     CDS             38..580
                     /locus_tag="ACHUN9_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0694449.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHUN9_000973"
     CDS             663..1364
                     /locus_tag="ACHUN9_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0694450.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHUN9_000974"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHUN9_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHUN9_000975"
     CDS             3979..5049
                     /locus_tag="ACHUN9_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0694451.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHUN9_000976"
     CDS             5061..5603
                     /locus_tag="ACHUN9_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0694452.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHUN9_000977"
     CDS             5611..6126
                     /locus_tag="ACHUN9_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0694453.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHUN9_000978"
     CDS             6092..6829
                     /locus_tag="ACHUN9_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0694454.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUN9_000979"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUN9_000979"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0694455.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUN9_000980"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUN9_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0694456.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUN9_000981"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUN9_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0694457.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUN9_000982"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUN9_000982"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0694458.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHUN9_000983"
     CDS             12136..14043
                     /locus_tag="ACHUN9_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0694459.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGC"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUN9_000984"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUN9_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0694460.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUN9_000985"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUN9_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0694461.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUN9_000986"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUN9_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0694462.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUN9_000987"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUN9_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0694463.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUN9_000988"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUN9_000988"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0694464.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHUN9_000989"
     CDS             complement(18711..20471)
                     /locus_tag="ACHUN9_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0694465.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHUN9_000990"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHUN9_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0694466.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUN9_000991"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUN9_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0694467.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUN9_000992"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUN9_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0694468.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUN9_000993"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUN9_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0694469.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUN9_000994"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUN9_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0694470.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHUN9_000995"
     CDS             complement(26072..26518)
                     /locus_tag="ACHUN9_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0694471.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHUN9_000996"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHUN9_000996"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0694472.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUN9_000997"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUN9_000997"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0694473.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHUN9_000998"
     CDS             complement(29281..29943)
                     /locus_tag="ACHUN9_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0694474.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHUN9_000999"
     CDS             30116..30529
                     /locus_tag="ACHUN9_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0694475.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHUN9_001000"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHUN9_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0694476.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHUN9_001001"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHUN9_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHUN9_001002"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHUN9_001002"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0694477.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHUN9_001003"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHUN9_001003"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0694478.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHUN9_001004"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHUN9_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0694479.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHUN9_001005"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHUN9_001005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8829 a         8131 c         8897 g         8275 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggttgc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aa
//
LOCUS       DAZOYS010000024        31072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOYS010000024 DAZOYS010000000
VERSION     DAZOYS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.024"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..232)
                     /locus_tag="ACHUN9_001006"
     CDS             complement(<1..232)
                     /locus_tag="ACHUN9_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HFR0694480.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLD"
     gene            complement(306..1250)
                     /gene="yfdV"
                     /locus_tag="ACHUN9_001007"
     CDS             complement(306..1250)
                     /gene="yfdV"
                     /locus_tag="ACHUN9_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0694481.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(1320..3014)
                     /gene="oxc"
                     /locus_tag="ACHUN9_001008"
     CDS             complement(1320..3014)
                     /gene="oxc"
                     /locus_tag="ACHUN9_001008"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0694482.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(3068..4318)
                     /gene="frc"
                     /locus_tag="ACHUN9_001009"
     CDS             complement(3068..4318)
                     /gene="frc"
                     /locus_tag="ACHUN9_001009"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0694483.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(4831..5454)
                     /locus_tag="ACHUN9_001010"
     CDS             complement(4831..5454)
                     /locus_tag="ACHUN9_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0694484.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            5751..6026
                     /gene="ypdI"
                     /locus_tag="ACHUN9_001011"
     CDS             5751..6026
                     /gene="ypdI"
                     /locus_tag="ACHUN9_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0694485.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(6103..6345)
                     /locus_tag="ACHUN9_001012"
     CDS             complement(6103..6345)
                     /locus_tag="ACHUN9_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0694486.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            6698..7618
                     /gene="lpxP"
                     /locus_tag="ACHUN9_001013"
     CDS             6698..7618
                     /gene="lpxP"
                     /locus_tag="ACHUN9_001013"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0694487.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            7974..8045
                     /gene="ypdK"
                     /locus_tag="ACHUN9_001014"
     CDS             7974..8045
                     /gene="ypdK"
                     /locus_tag="ACHUN9_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0694488.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(8110..9348)
                     /gene="alaC"
                     /locus_tag="ACHUN9_001015"
     CDS             complement(8110..9348)
                     /gene="alaC"
                     /locus_tag="ACHUN9_001015"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0694489.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            9725..11422
                     /gene="ypdA"
                     /locus_tag="ACHUN9_001016"
     CDS             9725..11422
                     /gene="ypdA"
                     /locus_tag="ACHUN9_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0694490.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            11437..12171
                     /gene="ypdB"
                     /locus_tag="ACHUN9_001017"
     CDS             11437..12171
                     /gene="ypdB"
                     /locus_tag="ACHUN9_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0694491.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            12184..13041
                     /locus_tag="ACHUN9_001018"
     CDS             12184..13041
                     /locus_tag="ACHUN9_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0694492.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(13044..15539)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_001019"
                     /pseudo
     CDS             complement(13044..15539)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(15564..16601)
                     /gene="ypdE"
                     /locus_tag="ACHUN9_001020"
     CDS             complement(15564..16601)
                     /gene="ypdE"
                     /locus_tag="ACHUN9_001020"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0694493.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(16601..17686)
                     /gene="ypdF"
                     /locus_tag="ACHUN9_001021"
                     /pseudo
     CDS             complement(16601..17686)
                     /gene="ypdF"
                     /locus_tag="ACHUN9_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(17701..18948)
                     /locus_tag="ACHUN9_001022"
     CDS             complement(17701..18948)
                     /locus_tag="ACHUN9_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR0694494.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(18970..19296)
                     /locus_tag="ACHUN9_001023"
     CDS             complement(18970..19296)
                     /locus_tag="ACHUN9_001023"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0694495.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(19516..20481)
                     /gene="glk"
                     /locus_tag="ACHUN9_001024"
     CDS             complement(19516..20481)
                     /gene="glk"
                     /locus_tag="ACHUN9_001024"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0694496.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            20685..21941
                     /locus_tag="ACHUN9_001025"
     CDS             20685..21941
                     /locus_tag="ACHUN9_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0694497.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            22056..22382
                     /gene="ypeC"
                     /locus_tag="ACHUN9_001026"
     CDS             22056..22382
                     /gene="ypeC"
                     /locus_tag="ACHUN9_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0694498.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(22523..23761)
                     /locus_tag="ACHUN9_001027"
     CDS             complement(22523..23761)
                     /locus_tag="ACHUN9_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0694499.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            24097..25299
                     /gene="nupC"
                     /locus_tag="ACHUN9_001028"
     CDS             24097..25299
                     /gene="nupC"
                     /locus_tag="ACHUN9_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0694500.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(25349..27537)
                     /locus_tag="ACHUN9_001029"
                     /pseudo
     CDS             complement(25349..27537)
                     /locus_tag="ACHUN9_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(27746..27821)
                     /locus_tag="ACHUN9_001030"
     tRNA            complement(27746..27821)
                     /locus_tag="ACHUN9_001030"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27786..27788),aa:Ala,seq:ggc)
     gene            complement(27861..27936)
                     /locus_tag="ACHUN9_001031"
     tRNA            complement(27861..27936)
                     /locus_tag="ACHUN9_001031"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27901..27903),aa:Ala,seq:ggc)
     gene            28157..28516
                     /locus_tag="ACHUN9_001032"
     CDS             28157..28516
                     /locus_tag="ACHUN9_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0694501.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            28518..28910
                     /locus_tag="ACHUN9_001033"
     CDS             28518..28910
                     /locus_tag="ACHUN9_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0694502.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(28962..30377)
                     /gene="gltX"
                     /locus_tag="ACHUN9_001034"
     CDS             complement(28962..30377)
                     /gene="gltX"
                     /locus_tag="ACHUN9_001034"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0694503.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            30636..30711
                     /locus_tag="ACHUN9_001035"
     tRNA            30636..30711
                     /locus_tag="ACHUN9_001035"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30669..30671,aa:Val,seq:tac)
     gene            30756..30831
                     /locus_tag="ACHUN9_001036"
     tRNA            30756..30831
                     /locus_tag="ACHUN9_001036"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30789..30791,aa:Val,seq:tac)
     gene            30878..30953
                     /locus_tag="ACHUN9_001037"
     tRNA            30878..30953
                     /locus_tag="ACHUN9_001037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30911..30913,aa:Val,seq:tac)
     gene            30958..31033
                     /locus_tag="ACHUN9_001038"
     tRNA            30958..31033
                     /locus_tag="ACHUN9_001038"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30991..30993,aa:Lys,seq:ttt)
BASE COUNT         7933 a         8031 c         7430 g         7678 t
ORIGIN      
        1 aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt gactgtccat
       61 ccacataggg accaaatgtg cgggtatcat caccatgacc tggtggctca actttaatta
      121 cccttgcgcc catattacaa agaagttgag ttccgaaagg tccattaagg acatgtgtca
      181 tatcgataac taataagcct tcaaacggcc ctttgctttc attatttgtc atttgcttat
      241 gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat caataacatg
      301 attaattatg aaaccagtcg actgacataa atccacaagg gagccgtgac gacaaagccc
      361 agtacgctca cagccaatga cgctgtacca gtacgggtgt agacattaaa ccggctggca
      421 ataatgattc cggagaatgc cggcggtaat gcgcctgcca gtaccatcat ctgcaaatgt
      481 tcgctgttca aatgacatgc cataccaacg agaagcagcg ccagtggcat cagaatcaac
      541 ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc agccagagtc
      601 aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt aaaggtcggg
      661 tcccatgctg ccggaatttt taccccaacc agcaccagga tcgttgccag aacaggtgcc
      721 catactactg gctcctttgc cgcagaaatt aatgcgctca gattactatt cttcttacca
      781 tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat tgcgttaaca
      841 ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata aataggatcg
      901 agaactgcaa accccaggaa tccaatggta ggtgaacccg caattaatgc acatacagcg
      961 gcttctgcat gggtacgttt aaaaaatttg tagcaaccaa accaggagaa aaagaaacat
     1021 ccgacaataa ccacaagtga tacaagggtc agacgagtgt ccgcaaaaat catttccctg
     1081 tttgcccggg taatagaaac aaatagagcc gcaggaagag cgtagtttaa taccagttta
     1141 ttaaatgcgc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa ataacccaac
     1201 agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat aatagtattt
     1261 ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga tgtcagtatt
     1321 taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc agttcctgca
     1381 gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc ggtggttaaa
     1441 gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa cgcatccatt
     1501 aatttgtcat accttgcatg gtgcaacaga tccgttggtg atggtgcacc agcgccactg
     1561 agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat cgtcaccggc
     1621 aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc actatcacct
     1681 tcaatggcga caaccggaga accagaagtc acgctagcac cgatggcata gcccataccg
     1741 atgcccatga caccccaggt accacaatcc agacgacgac gtggtttata catatcaata
     1801 atatttcgtg cattatccag ggtatttgca ccttcattaa ctaaataaat atcctggttc
     1861 tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt aatttaatgg ttgagtatct
     1921 gtacttaatt tttcatgcat tttttgtgca ttttgctgct tgtggatatt taaaatatcg
     1981 cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat accttgcata
     2041 ctggatgcaa tatcaccaac gactggcaca gcaatggggc ggttgctgtc aatttcctgc
     2101 ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt accgtgtgcc
     2161 aataaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc cagggcaaac
     2221 gaacgcgcag ctgccgcaga aagtggatgc gtatcttcaa ggatcccttt cgccatagac
     2281 attggcagga atggaatctg agcactttca ataaattcac gaagctgttc atcagcttgt
     2341 gaatacgccg cgcctttgcc aaggataatt aatggccgtt cagcttttgc taaaagcgaa
     2401 attgcgctag tgactgactt cgggcatggc aataatgctg gcgacggatt ttcaacttta
     2461 acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt tgctggcaaa
     2521 tcaagataaa ctccgccagg gcgacccgat acagagaccc ggatagcgcg tgccaatgca
     2581 atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg ttttgccgca
     2641 ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc gcggtcgctg
     2701 gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa tgcggtcaaa
     2761 ccattgagga atcctggcgc agaaactgtc aggcagatcc ccggtttttg ggtaagaaaa
     2821 ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat ataacgaatg
     2881 ccttccgcct gggcatggcg tgccatatcc gtcacaggaa tacctacaac accataaata
     2941 gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc tgtcatttga
     3001 agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc agaagccccc
     3061 gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg ctataaccca
     3121 gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg cgcagcttta atatccggcg
     3181 taaaggcaga gaatttcatt ggacaaccaa cggtcagata ttttccacgc aacggttgtt
     3241 ccacttcaac aacactgcca ctttggcgca aagagggatc aagtgaaatt tctttcatac
     3301 ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccaccgct tcatgtttat
     3361 caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt ggctgtcggg
     3421 catgggctgt actgtatgcc gaatcggtaa tccattctgg tttgccgatg gctttacagg
     3481 tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga tcggtttccc
     3541 agcctttaca tttcaggatc cagccaggct gaccgccacc acctgcatta ccaccgcggg
     3601 gaactgcatc accaaatgta ccattcggat actgcgggta ttcttccaga taacccaatt
     3661 tatcgagacg ctgctggtca cgtaatttca cgcggcaaag gttcaatacg gcatcctgca
     3721 ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa gcagcaagta
     3781 aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg cttaccagcg
     3841 gcggaccatc ccaaaaaccc gtagtggatg ccgcgccacc cgctgcctga gcaacgtttt
     3901 cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc gaaccaaaaa
     3961 tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatgtga tcaatggccc
     4021 ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc attacctctt
     4081 tgccttccgc tgttttggta tttaactcaa tagaacgttt gttactgtta agcatggtga
     4141 agtaaagcgc atcgatatca ggaatatctc gcagttggtg acgcgttacg tcaccaacgc
     4201 cgggacgttc aattttaata acgtcagcgc caaaccaggc cagcatttga gtacaagatg
     4261 ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga gttgacatag
     4321 caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg cataacgccc
     4381 tctataaata atctgcaaaa tatcttcgta gcggcactcg atatttttaa attcctgtat
     4441 caagtactga aaattatttc atcgccactt ccaattcctt tctgcatggt aagtagtgag
     4501 gacgaaaaga agaatatgat aaatatttat tcgtggtaat cagagagagt cgcgacattt
     4561 gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaaga gtatgattac
     4621 gctttttaaa gcgtaccata aagcataaca taataaatga tttgtgatga cactggatta
     4681 ttgcggttta ctttgaggaa aataaaatgc gtgtttatag aaccgcgagc gccaaataat
     4741 tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga gggagttaaa
     4801 tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt cgctatcgac
     4861 gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt gctttttatc
     4921 aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca tcagatattg
     4981 gttttccatt acgccaacac cggccaggcg aagctcttcc attgcgccct ttttatcccc
     5041 tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggtg ttgctacata
     5101 actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat tgagattggt
     5161 ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca gcaaagcgga
     5221 agcttcattg gtcagttctt ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc
     5281 tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg ctgctgccgt
     5341 tttctgggtt tgctgagttt gctgttgtgc cgctaccggt gcattatcag cagcccatac
     5401 agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt tcatacttac
     5461 ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat gaaatgagaa
     5521 atcagagagc aacggtcatt ttgaaagggt tatcttacaa ttgtagttat tatcgcaaag
     5581 cggcatttag tcttacagtt gtaggaatat gcttcctgtc gcagagaact attcttatat
     5641 accacaaaga gatgtcattt attatattga tgtaaatatt gattaattat atgtaaatac
     5701 agccataaca tcgtatggtt ctcagtgaac cattttcagg ataactttct atgaaagtaa
     5761 acttaatact tttcagctta tttttattgg tctctattat ggcatgcaat gtttttgcat
     5821 tttccatttc tggtggtgta agtgagagga gctataaaga gactgaaaaa acatcagcga
     5881 tgacaaccac acactctaca aaacttcagc catcacaggc gattttgttt aagatgagag
     5941 aagatgcgcc accattaaac ctcacagaag aaatcacgcc tacttatccg acaaaggcga
     6001 attatcttat tcatcctgtg cgatagcgtc tgtcacatgg caggtgaaca atcatgtctg
     6061 atgctgatgt gaacacctgc ccaaatgagc catcctgacc cactatcgcc tgccataccc
     6121 caccataaag cgaatcatca agccacaagc cggggcaaga aacataaata tccccgtcac
     6181 cagttcatta ccgccagtta accacatggt gaagtaataa atgagtgcag cgaggatcac
     6241 catgccgaag aaagaagaga ccgccgatac cacgttcagt acctgaccga tgtagccgct
     6301 tacgcagaca atacacaggg cgagaaacat ccataaataa atcatagctc aacctcctgc
     6361 cgcgctaaaa aacctgtaac ttcagtcact ttaatagtaa gacaatcctt aacctctggc
     6421 aatttttgct aagcatcaat gaaaacagca cgttaaatgc aacgctattg cgtgcggaaa
     6481 attttttact ttgcacgatt aataatcagt agctgaaagc agtcagccag tgataaagta
     6541 gagccaccgc attcatgctc ctcgtggtta tgtcctgact agtctttaca ctctttacag
     6601 gaaccattgt cgtacatgat ggcccaacca attgagtgta ccgctgtcga catggaatct
     6661 tctgcgatac aacaattcgt atctacagaa ggtaactatg tttccacaat gcaaattttc
     6721 ccgcgagttt ctacatcctc gctactggct cacatggttt gggcttggtg tactctggct
     6781 ttgggtacag cttccttatc ctgttctctg ctttctcggc acgcgtattg gcgcaatggc
     6841 gcgaccattc ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac tttgtttccc
     6901 gcagcattct gcggaagaac gcgagaagat gattgccgaa aactttcgtt cactcggcat
     6961 ggcgctggta gaaaccggca tggcatggtt ctggcccgac agtcgcgtac gtaaatggtt
     7021 tgatgttgaa gggttggata accctaaacg cgcacaaatg caaaatcgcg gcgtaatggt
     7081 tgtcggcgtc cattttatgt cgctggaact gggcggccgc gtgatgggac tgtgccaacc
     7141 aatgatggct acctatcgtc cacataataa tcagctgatg gaatgggtgc agacccgtgg
     7201 gcgcatgcgc tctaacaaag cgatgatcgg cagaaataat ctgcgcggca ttgtcggtgc
     7261 actgaagaaa ggtgaagcgg tatggtttgc tcccgatcag gattatggtc gtaaaggcag
     7321 ctccttcgcg ccgttctttg cggtggaaaa tgtcgccaca accaatggca cctatgttct
     7381 ctcccgtctc tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag cggattacag
     7441 cggatatcgt ttgttcatca ccccagagat ggaaggctac ccgacagatg aaaatcaagc
     7501 cgctgcctat atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg agcagtacct
     7561 ctggatccac cgtcgcttta aaacgcgccc ggtgggagaa tcgtcgttgt acatttaaca
     7621 aactgctctt actctttccc tcctcaaatc ggccataata acctcatggt ttttatggct
     7681 tttttcttaa ggttttttcc ttcgctgaat atctaattat taccgtcaga aattaaactg
     7741 tcgccgatat gagacacttg taagttacta aaagtaaata agaaaatgcc tcttgtctcg
     7801 tttcagtttt aggcgtacat taacgccgtc ttgagcagtt tagctcagaa ttctggggtg
     7861 atgcccacgg taaaatacag ggcttttaag gtcgctttgc ccggtgtcaa ctcactgtat
     7921 caggtgtaat gaagtcattc aggcgtaaca gtaattacgc ggagagatgt aaagtgaaat
     7981 atttctttat gggcatttct tttatggtca tcgtttgggc cggtactttt gccctgatga
     8041 tctaaaagca aactgtgcaa tgaaaaacag gagccatcag gctcctgttt gcatttctga
     8101 cgacggtgtt tattccgcgt tttcgtgaat atgtttgctg ctggcgggta gtaaaccgtc
     8161 ggcacggaac atcgctttaa tccctctgat ggcctgacga atacggtcgc ggttttcaat
     8221 cagtgcaaag cgaacatgcg tatcaccata gtcgccaaag ccaatccccg gcgagacaca
     8281 aaccttcgct tcgttaagga gcttcttggc aaattccagc gatcccatgg ccgcatatgg
     8341 ttccgggatt ttcgcccaga catacatcga agccttcggc atttcgacca tccagcccgc
     8401 ttcatgcagc cctttaacca atacatcacg gcggcgtttg tactgttcgg taatgtcgcg
     8461 cacgcactgt tgatcgccct cgagtgccgc aatcgccgcc acctgcaacg gcgtaaaagt
     8521 gccgtaatcg tgatagcttt tgattcgcgc cagggcactg accagcgttt tgttgccgac
     8581 cataaagccg atacgccagc ccgccatgtt gtagcttttc gacagcgtaa agaactcgac
     8641 cgccacatcg cgtgcacccg gcacctgcat aattgacggc gctttccagc catcgtagac
     8701 gatatcggca taggccaggt catggaccac cagcacatcg tagcgtttcg ccagcgccac
     8761 aaccttttcg aagaactcca gttccacgca ttgcgcggtt gggttcgacg ggaagccgag
     8821 gatcatcatc ttcggtttcg gataactttc acgaatggcg cgctccagtt cgttgaagaa
     8881 atcgacacct tccaccagcg gcactgagcg tacctgcgcc ccggcaatca ccgcgccata
     8941 aatatggatc gggtaacttg gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc
     9001 cagcatcaga tgcgccaggc cctctttcga accaatagtg acgatggctt ctgattccgg
     9061 gtcgatttca acgtcgtagc gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg
     9121 aatgccgcgt gaagtggagt aaccatgcgt gtccgggcgc tgggccacag tacataattt
     9181 ttcgacgata tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat cgataatatc
     9241 ttcgccgcgc cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg
     9301 gagacgatca atgcgcgtaa agcgacgttc agggcgagtg tcagccataa tttcctcaga
     9361 ttaacgttag cgcccggacc gtccgagcga cgctgccaca atggtggctg ttttgaaaat
     9421 agcctgatta atttctgtct gtccagcgtt ttagaacatt tttatcaatg gctttaaatg
     9481 aggaactcca cttcaaaaac aggaaatttt tctacgctaa caagaaaatt accaccaatt
     9541 ttattaacat tataatttca ataaatttac cagccagaag tgcattatca agagatacat
     9601 tgctaataca ccatttatct ttcaatcccc ttttaaattt atggtttttc ctcaaaaacg
     9661 gcctgctggt catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc
     9721 acccgtgcac gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct
     9781 tatctgcctg ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc
     9841 gcactcccca aaagaattgc tcgccgttac cgccattttc tcgctgttcg ccctgttcag
     9901 cacctggtcc ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat
     9961 gtccgacggg attctgtttg gcccgtgggt aggcatcatt accggcgtga ttgcgggtat
    10021 tcaccggtat ttaattgata tcggcggcgt gacggcgatc ccctgcttta tcaccagcat
    10081 tctggcgggt tgtatatcag gctggataaa cctgaaaatc cccaaagcac agcgctggcg
    10141 cgtcggtatt ctcggcggca tgttgtgtga gacgttgacc atgattctgg taattgtctg
    10201 ggcaccaact accgcgttgg ggatcgatat cgtctctaaa atcggcattc caatgatcct
    10261 cggtagcgtc tgtatcggct ttattgtgct tctggtgcaa agcgttgagg gcgaaaaaga
    10321 ggccagcgcc gcgcggcagg ccaagctggc gctggatatc gccaacaaaa cgctaccgct
    10381 gtttcgccat gtcaatagcg agtcattacg caaggtctgc gaaattatcc gcgatgacat
    10441 tcacgccgat gcggtggcga ttactaatac cgatcatgtg ctggcctatg ttggcgtggg
    10501 tgaacataac tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa
    10561 ttacggaaaa atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc
    10621 catgctggtg atcccattgt gggagaaagg ggtcgtgacc ggaacgctga aaatttacta
    10681 ctgccacgcg catcagatca cctcgtcatt acaggaaatg gcggtcggtc tgtcgcaaat
    10741 catctccacg caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc
    10801 agagcttcgc gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg ctctgaacgc
    10861 tatttcatcg tcaatccgtc tgaatccgga taccgctcgc cagttgatct ttaatctgtc
    10921 gcgttatctg cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga
    10981 gctgtatcaa attaaagact atattgccat tgagcaggcc cgctttggtg acaagctgac
    11041 ggttatctat gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc
    11101 gttggtggag aacgccattg tccacggtat tcagccttgc aaaggtaaag gcgttgtcac
    11161 catcagcgtt gcagagtgcg gaaatcgggt acgcattgcg gtgcgagata ccgggcacgg
    11221 catcgatcca aaggtgattg agcgggtcga agcgaatgaa atgccgggca ataaaattgg
    11281 cctgctgaat gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg
    11341 cctggagccg gggacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc
    11401 acaggctacg ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac
    11461 gaattcctgg cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt
    11521 gtcggcacct ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac
    11581 gccatttttc tggatatcaa tattccgtcg ctggatggcg tgttgctggc gcaaaacatc
    11641 agccagttcg cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggta
    11701 gaagcgtttg aactggaggc gtttgactac attctcaaac cgtatcagga gtcacgtatt
    11761 accgggatgc tgcaaaaact ggaagcggcc tggcaacaac agcagaccag cagtacgcct
    11821 gccgcgacgg taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc
    11881 gtcacgccaa ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtctat
    11941 acgcggcgtg aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg
    12001 ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatacgcgaa
    12061 atcgaaccgt ggtttaataa cacctacatt ctgcgactga aagatctgga ttttgaagtg
    12121 ccggtcagcc gcagcaaagt gaaagaattt cgccagttaa tgcatcttta atcgggaact
    12181 ttcatgaaag caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc
    12241 ctggtgctta acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgtta
    12301 ccggtgggca gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac
    12361 ggcaacctgc tggaagcaaa gcagcagcga atggtggaag gagaaatgct gtttattccg
    12421 gcacgcgcgg ctaatttacc gatcaacaac aaaccggtga tgctgttaag cctggtgttc
    12481 gcaccgacct ggcttgggtt atcgttttac gatagccgca ccacgtcgtt gttgcatcct
    12541 gctcgccaga tccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc
    12601 ctcacccatc ttagccgttc gccgctggag cagaatatca ttcagccact ggtgttaagt
    12661 ttgctgcatc tttgccgcaa cgtggtgaat atgccgccgg gtaattcgca accgcgcggc
    12721 gattttctct atcacagcat ttgtaactgg gttcaggata attatgccca gccgctcacc
    12781 cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct
    12841 cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg
    12901 atgattttgc agaaatatca tctgtcaatt catgaagtgg cacagcgttg cggttttccg
    12961 gatagcgact atttttgtcg cgttttccgg cgtcagtttg gtctgacgcc gggagagtac
    13021 agcgcccgtt ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata
    13081 ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc
    13141 gccagctgcg agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg
    13201 atcaccagtt caacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca
    13261 atgctgatac tggaatgacg gatccactga gatttggtgt gcgggatcgc tacgccaaaa
    13321 ccaacgccgg tggtaacaat ctcttcccgc tgccagacat cttcttccag ctcaaacgga
    13381 tgttcagtgc gcccgttaac gccgaggttg ccgcacagga actggatcgc ctgctcttta
    13441 ttgctaaaat cctgatcaac aaagatattc tccagcgcca gcagtgggcg aacgtcttct
    13501 tccggcgtaa aggcggtgag taacgcttca atttcctgcg cactgcggca ttcacatgcc
    13561 tgacgcgcca gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc
    13621 ggaatacgcg ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga
    13681 taacggcttt caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca
    13741 gtggtaacta tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc
    13801 gatacgcgcg ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg
    13861 atactgaaga aatcgacctc atcggagaag tggtcgatga tgtagcacac cgacggcact
    13921 tccaccatga tcccaagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca
    13981 acgatcgctt tttggatctc gcctttcacc cataagatct gatcgaggct gtgaaccatc
    14041 gggatcatca actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg
    14101 cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaacgggttc
    14161 tcttcctggg gaatattcag ataaggaatg cttttatcgc cgccgatgtc catcgtgcgg
    14221 aagataatcg gcttgtcgcc cgccgccagt agcacctgct ggtaggcttc aaattgctcc
    14281 tgctcgtccg gcgcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgaca
    14341 ccttccgcgc cgttggcaaa cgcgcctggc gcttccagag cggtgccgat attcgccgca
    14401 atatcaatac gcttgttatc acgggaatag gccagctgcg cggcagcctg cgcctgttgt
    14461 ttttggcgtt tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc
    14521 gggttaatcg ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgg
    14581 gcaatcgcat ccagcggcaa gccactcagt accgggatcg ccgaggcacg ggccagaatc
    14641 agtgtatgcg aggtgcgccc ggttttctcc agaatcatgc ccgcaagatt tttcaaatca
    14701 aggctcaaaa actggcttgg ggttaaatct tcagccacca gaatggtcgg tttttccagc
    14761 accagattgt tgcgcggctt cagttccggc taggtgatat gcagcaactg ttcgctgata
    14821 tcgcgaatgt cgctgacccg ctcgcgcaga taatcgctgg cagaagcaga tagtttggcg
    14881 caaacctgct ccatattgct gatgatcgcc gcccccagtc cctgatgctg ttctgtcatc
    14941 aggcgacgga tattgcctgc aaattcatca tcctgaatca gcgacaaatg agcgctgagg
    15001 atagttttgc tttcgccgtc acgctcacgc aattgctggt tcagttgctc ggcaagcgtt
    15061 gccaggctgt gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg
    15121 tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca
    15181 ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc
    15241 agttctgcct gcgtcggctg aacgctgtcg ctgtcgataa agcgcaccag gatgtactct
    15301 tccagcacgc gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca
    15361 ttaaataagg tgccagttcc aatcaacgcc agcgagcttt tagcatcggc ctttgcgttc
    15421 tggcgatggt tgataaaggt gatttcgctt tgccactggc tgcactgttc tttaagttcc
    15481 cacgccggac gagcatgtag accgttaggc aaaggacaga gaaattgaat cgttaacata
    15541 accgctccgg gttaatcagg agatcatctg aaatccgtca gttgaacaac cgtctcacgc
    15601 gtaagacgtt gaataagggc agataaaagt tgctccatct gcaaaatgtc gcggcaatcg
    15661 gcaatcgatg ccgcgcaatg tccgtggcgg gtggcaggcc ccattaccac tgtgggtacg
    15721 ccagtatcgg ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa catatccgcc
    15781 tgcaacggaa cgccaatttc tgccgacacg gtttcaatcc acgcggtaag ttttggcggc
    15841 gcaatcagcg atttatcgct taacaccagc atcggcccgt taccaatctg acgatggtta
    15901 gccgcgccat aatcaaagtt tttcgcccag caggcggtat caagcacaat ggcgacgtcc
    15961 ggcgacaccg cgcgggtagc agtttgcccg ccgcgtaatc ccacctcttc gctggaactt
    16021 gccaccagcc acacttccgc aggcagttcg gcgtcgtgca gctcacgtag taacgtcacc
    16081 agcagatagc aaccgaggcg gtcatcaaag gctttcccca tcacccgctg gtgagggaga
    16141 acctgaaaag ttgtatcaaa ggtgacgcga tcgcctggac gaattcccgc ctgcatcact
    16201 tcgtcatacg agcgcgcacc aatgtccacg cgcatggcgc tgacgtcatt cccctgccgg
    16261 tcgccgtcaa gcaggcctgg aattttgcac tcttcacggg tggtgatgcg caccggctgc
    16321 agctggcggg cagccatgcg cacgttgcca accggcagca catcaattgc cccttcgcgg
    16381 gagatgctgc gcaccataaa tcccacttcg tccatatgcg cacagatcat caccttcgga
    16441 cctgtcgatt cattgaggcg gatcagcacc gatcccagac catcaaatcg tacttctttt
    16501 tgcaggcgat ccgcttcttc cagtaggatc tgccgcactt cctgttccga ggaggcgatc
    16561 gcatctgcct cgctcaacgc ttttaatagc gataaatcca ttatgcctct cccgtgagca
    16621 acactgtttt cggcatggcg tagagcactt ctgcgccttg cggggtaacc agcacaacat
    16681 cttcgatgcg cacgccccct tgccctggca aataaatccc cggctccacg gtcagtaaca
    16741 tgcctggctg tagcgtcgtg gtatcccgcg gtgaaaagcg tggatcttcg tgtacttcaa
    16801 taccgatagc atgaccggtg ttgtgaccaa aatagtcgcc aaatcctgcc tcggtaatca
    16861 cccggcgcgc ggcgtcgtca acctgttagc agcgtacgcc agggcgaatc gcggagattg
    16921 ccgccagctg tgcttgcagg acaatctgat agacgttaaa cagcgggtga gattcggcaa
    16981 ttaccccttc gccattcacc agcaaggtgc gcgtcatatc agagcagtac ccctgataga
    17041 gcgcaccaaa atcaagagtg acaaactcgc ctgctgcaac aatcttgtcg ctggctttgc
    17101 cgtgcggcag cgccccacgc cagccgctgg cgacaatggt gtcaaaagag gctttttctg
    17161 cgccctgctg gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg
    17221 cctgaataaa gcggcgaata tgctccgctc cgcgatcggc aatcccacag gcgaggcgga
    17281 ttatctccac ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca
    17341 gtttcgcatt gagttcagac ttccagcggt gcgcggtttc ccaactcacc tgctggcctt
    17401 caaaaccgag cgtttgcaac tgctcatcgg caatgatttg attgacgata gtggtaagcg
    17461 tgttcgtcgc gtcaagcaaa tgcagctggt agccttgcgc acgggcttcc acctcaacga
    17521 aatagcgcga atctaccaga atgtgcgcac tttcacggct aataaccaca tagcctgatc
    17581 cggtggagat ccccagatgc ggctgtttgt tctgccgtga ggagagaagc actgcatcca
    17641 gttgttgcgc cttaagccag tcgcgcagcg aagcgagtaa tgtcatctgg attttccttc
    17701 ttacaggcta tcgattaaca atttaccttt acggaacatc atcagacgca ggaacaccac
    17761 catcagcgca gttatgactg cccccagcgc gatccccgcc atatagacgc ccaggttagt
    17821 gaccagcggc catgcccaaa tagcggattc cgggaaccat tgcactgcgc ccagccagac
    17881 agcggcggtt gagccgacaa tcgcgccgac catatacgac ggaatggcgg tgatggggct
    17941 ttccagcgca aacggaatcg caccctcact aatccccata aaggcaagga acatcgcggt
    18001 cttaccctgc ggataaagtt gagcgttgaa caggcgtttg ccggttaaac gacggtcgat
    18061 aatggtcgcc agacccagac ctatcggcgg aataacgata gcgatagaac gtgcagtgac
    18121 cggtaatacg tggtcagtgg taaagctgaa ggcaacaaaa cctgcggctt tgttgatcgg
    18181 gccaccaagg tcgattgcgg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt
    18241 ctcacctgcg gcagtcagca cggtacggat accgccgttg atccagccgc caaagggggt
    18301 aatgacgtag tacatcgcca gcatgacaaa aatggcggaa agaatcggta gcaaaaatgt
    18361 ggttttgaac gctaacagga aatccggtaa ctggattttc tggttcatcc acttcacaag
    18421 gtaacccgcg acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga
    18481 ggtcgcccat tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat
    18541 caagccgccg ataaaaccag ccgggaacgc cagtttgcca ccgatagagt tcgccacaaa
    18601 agcagcaaac atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacagcca
    18661 ggcaaatttc agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa gagcatccat
    18721 gatgccgatc tcagcaggaa ttttaagcca gctataagca atcagctggc tgaaagcgag
    18781 gatcacaccg cccataatta aggtcggcac catgcgtgaa atccctaaca tcacatgctg
    18841 cgggagttca ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc
    18901 accggatgcg ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc
    18961 tgtcgaatat tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc
    19021 agcgttttta attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgctc
    19081 attatcttcc ggggtaactg ccacggagtg gatgatgatg gttgcttcgg cgatatcctg
    19141 cgccgtcagg cgattctgga taccgtccgc gccctgggtt tcaattttca cttcataacc
    19201 tgcttctacc gccgcttctt ccagcgcctg agcggccata aaggtgtgag ccaggcccat
    19261 tgggcaggca cataaggcaa ttagtttctt actcatcgtt aattcctcgt tcagtgatag
    19321 ttgactagtg aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt
    19381 tactggagaa ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgagggggac
    19441 acaaagaaaa attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga
    19501 tataacagaa aggatttaca gaatgtgccc taaggtctgg cgtaaatgtg caccggaacc
    19561 gagaagaccc ggattgtcat ggacgatgag atacaccgga atatcatgga catattcttt
    19621 aaagcgccct ttatcttcaa atgcggcacg gaaaccagag gctttgaaga actcaaggaa
    19681 gcgcggcacg ataccgccag caatataaac cccaccaaag gttcccagcg ttaatgccag
    19741 attgccgcca aaacggccca taatgacgca aaacagcgac aacgcacggc ggcaatcggt
    19801 gcagctgtca gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg
    19861 gttgtcggct ttaacaattg cgcgatacaa attcaccagc ccaggaccag aaagcacgcg
    19921 ttccgccgaa acatgaccaa tttccgcacg caatatttcg aggataatgg cctcttcttc
    19981 actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc
    20041 gacatggacc agatgcgcaa ccccaagccc tgttccggca ccgtaaaccg caataggttt
    20101 accttcgacc ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat
    20161 cgccatcgat acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt
    20221 tttcatttcg gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt
    20281 aattgggcaa gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata
    20341 aacgcgaatg accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga
    20401 gatttcacca ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc
    20461 gactaatgca tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc
    20521 acactgtaaa tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat
    20581 cgatctgggt cacacattta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc
    20641 aagctgggca aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag
    20701 ccagaaccat gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta
    20761 ttctgattgt ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc
    20821 cgggaactct ggggatagcc caggattcac ctatctggat catcggtgta ttaacgctaa
    20881 cgggtattgc ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg
    20941 cctgtgaacc gctgatcggc gcaccagttc cgccctctgc gctaccagga cttatcgtag
    21001 cattaattct cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg
    21061 tcaatatcgc ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg
    21121 agtggactat tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg
    21181 cggcgttgat attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc
    21241 tttttgcgcc gttaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc
    21301 cccatttttc actgcccatt gcgcattacg gacagatgga aatgaccgat attctcagcg
    21361 gtgcaattgt cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac
    21421 ggttgcacgc gatgatgaat caaatgaaaa atccggtgct cgtgctgggt attggcggat
    21481 ttattctcgg aattctgggg gttattggtg gaccagtttc gctgtttaaa gggctggatg
    21541 agatgcagca gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg
    21601 taattaaact tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct
    21661 tcccggcagt gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg
    21721 ttccagcggc aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg
    21781 atggctggtt aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc
    21841 tgctctgtat cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg
    21901 tcaatcgtcc gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctctccttta
    21961 tgatttaaga gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag
    22021 gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg
    22081 ccgccctgat ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat
    22141 cgatcaaatt acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact
    22201 ggcgagatcg tgattactgg catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc
    22261 atgataatgg ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg
    22321 aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt
    22381 aaacgcgtcc tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct
    22441 acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt
    22501 gccagatgcg acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat
    22561 tcagcgccac gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca
    22621 ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg
    22681 cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca
    22741 tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac agcgggatat
    22801 ggaagcgaat gaatccctgc atcaccacct gccccgccag tgtccccacc accgttgagg
    22861 acagtccggc agcaaccaga cttaacccaa agaccgttgc cgcagcatgg cttaacagcg
    22921 gttgcagcgt cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga
    22981 acgccgccgc agctgtagcc atcatcgcca gattgacaaa accggcaatc gtcatggcga
    23041 tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct
    23101 gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg
    23161 ctgccaggaa gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct
    23221 gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca
    23281 gtaacccgcc aatcactttc tccagtggtt tttgcccgcg acgttgcagc attaaaatca
    23341 ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgagacacca agaatgagtt
    23401 taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg
    23461 cctgaaccca atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca
    23521 gattttttcc ggtggcaatc cctagtttgg cagagaggat ctgaatcagc atcgccatca
    23581 ggttggccca aacgacaacc cacagtagct gatagccgaa gctggcaccc gcctgaatat
    23641 tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta
    23701 atgcgagcct catcttgcgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca
    23761 tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgataaac gagaatgatt
    23821 atcaaattca tttaaatgga ttgtggtgat ttctctgata gaccaggatt atgactacga
    23881 aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc
    23941 agcttccata cacaacatag cagaaatgta tgacagatca ctatttttga agcctgtcac
    24001 aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg
    24061 cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta
    24121 ctggcacttg ccgttgttgc gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt
    24181 cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg
    24241 aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc
    24301 agatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca
    24361 ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc
    24421 cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc
    24481 aacggtatgg gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtct
    24541 gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc
    24601 atggctgcca cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg
    24661 ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg
    24721 ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac
    24781 ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa
    24841 gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac
    24901 gcactgtttg ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg
    24961 ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa
    25021 gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg
    25081 cagaaaattg cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctggtt
    25141 tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa
    25201 gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg
    25261 agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa accatacata aaaaagccgg
    25321 ggatcattcc ccggcttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca
    25381 aatacccttg cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct
    25441 cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca
    25501 caatcatcgc atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga
    25561 tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg ccaaagtcat
    25621 caatcgcaat ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg
    25681 cgttagaaaa cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat
    25741 aacgcttaaa cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca
    25801 ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca
    25861 acaaggattc cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg
    25921 gcagaaactt atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat
    25981 cataaccttc accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat
    26041 ctaacgccgt gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca
    26101 tctcctctct gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc
    26161 agcttaactg ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg
    26221 cagcaccata gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga
    26281 tgttaaccat atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt
    26341 cactacccgg caattgatat aacttttcgt tttcctgcat caacggcagc agcgtacggt
    26401 aaattgatcg gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa
    26461 gattatcaat gcgcaggcag caaaactctt gcccgcctct tgctccggcg cttgttccaa
    26521 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
    26581 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
    26641 agtcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
    26701 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
    26761 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
    26821 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
    26881 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
    26941 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
    27001 gtaaataagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
    27061 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
    27121 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
    27181 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
    27241 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
    27301 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac cgcgccagcc
    27361 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
    27421 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
    27481 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
    27541 tacactactc tcactgatcg gggttttcag gcacacaaat aataagttta ttcgcatcgc
    27601 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
    27661 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
    27721 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
    27781 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
    27841 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    27901 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
    27961 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
    28021 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
    28081 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
    28141 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
    28201 cggctatagc cgccagacca ttaataaatg ggtacgcaag gagggctgga caacgtcacc
    28261 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
    28321 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
    28381 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
    28441 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
    28501 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
    28561 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
    28621 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
    28681 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
    28741 aagagagaat tgctgaatac gctcctggag cacgcgcccc ggcgtatcga caaatcatca
    28801 acgcgataga taatatgaca gacattgagc agggaaaagt cgcgcaattt ttgtcacgtg
    28861 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
    28921 gcaggatatt atctcctgcc gtttatcttt ttacacgcta attactgctg attttcgcgt
    28981 tcagcaataa aatccagcgc tttgttgata cgctcgatac tgcgggtctt accaattgcg
    29041 tgaacggtaa catccagtgc tggagactgc cccgccccgg ttacggcgac acgcagcggc
    29101 ataccaactt tacccatacc cacttccagc tcatccgccg tcgcctgaat agcgtgatga
    29161 acgttttcag cggtccagtc agtaatcgcg gccagtttgt cacgaaccac ttccagcggc
    29221 tgacgcgcta ccggacgcag atgttttttc gccgcatcgg catcgaactc agcaaaatct
    29281 tcgtagaaat aacggcagct ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc
    29341 agtttcacca gatcagccag ctgcgggccg ttacgggtat cgatattttc ctgctcaatg
    29401 tgccactgta agtgagtagc aacatactcc ggcggcagcg cgttaatgta gtgatggttc
    29461 agccacagca gcttgtcggt gttgaacgca ctggcagatt tgctgacggc attcaaagtg
    29521 aagtatttga tcatctcttc acgagtgaag atttcctgat cgccgtggga ccagcccaga
    29581 cgcaccagat agttcagcag tgcttctggc aaataaccgt catcacgata ctgcattacg
    29641 ctgactgccc cgtgacgttt ggacagtttt ttaccgtcat cgccattgat catagaaacg
    29701 tgcgcgtaaa ccggcaccgg cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg
    29761 ttgttgatat ggtcttcgcc acggataacg tgggtgattt ccatatccca gtcatcgaca
    29821 accacacaga agttataggt tggggaacca tcggtacggc ggataataag atcgtccagt
    29881 tcctggttgc tgaactcgat cggaccacgg atctgatcgt caaaaacaac agaaccttcc
    29941 tgcgggttag caaaacgtac aacacacggt tcatcatcag catgatgctc atggctgtgg
    30001 cggcagcgac cgtcataacg cggcttctca cctttcgcca tttgctcttc gcgcagcgct
    30061 tccaggcgct ctttagagca atagcattta taagcagtgc cctcttccag catctgatcg
    30121 atcaccgcgt tgtagcgatc aaaacgtttg gtctggtagt acggaccttc atcccactcc
    30181 aggctcagcc agttcatgcc atccataatg gcttcgatag cttccggcgt ggaacgctca
    30241 agatcggtgt cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag
    30301 gagtaaagag cagtacgcgc gccgccaacg tgcagatagc ctgttgggct tggcgcgaag
    30361 cgagttttga ttttcatgaa atggccttac gtttagaaag atgccgacaa ccggcaaatc
    30421 ctggaaaaat tgagtgggcg atattctatc actcccgcct gattcctcaa tgtagttcgg
    30481 gtttctacct tacgcattat tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg
    30541 atcatcttgc ttaaatttac gacgaacgaa caatttcttt agaaaatgcg ttgactcatt
    30601 ttgaactctc cctataatgc ggctccacac agcgggggtg attagctcag ctgggagagc
    30661 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
    30721 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
    30781 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aatttctcgc
    30841 cagctaaatt tcttgtaaaa atgtgaagta ccgaagtggg tgattagctc agctgggaga
    30901 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg
    30961 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
    31021 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tg
//
LOCUS       DAZOYS010000025        30911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOYS010000025 DAZOYS010000000
VERSION     DAZOYS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.025"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            511..1026
                     /locus_tag="ACHUN9_001039"
     CDS             511..1026
                     /locus_tag="ACHUN9_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0694504.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1244..1972
                     /gene="artP"
                     /locus_tag="ACHUN9_001040"
     CDS             1244..1972
                     /gene="artP"
                     /locus_tag="ACHUN9_001040"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0694505.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1990..2721
                     /gene="artI"
                     /locus_tag="ACHUN9_001041"
     CDS             1990..2721
                     /gene="artI"
                     /locus_tag="ACHUN9_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0694506.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2728..3444
                     /gene="artQ"
                     /locus_tag="ACHUN9_001042"
     CDS             2728..3444
                     /gene="artQ"
                     /locus_tag="ACHUN9_001042"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0694507.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3444..4112
                     /gene="artM"
                     /locus_tag="ACHUN9_001043"
     CDS             3444..4112
                     /gene="artM"
                     /locus_tag="ACHUN9_001043"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0694508.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4404..5135
                     /gene="artJ"
                     /locus_tag="ACHUN9_001044"
     CDS             4404..5135
                     /gene="artJ"
                     /locus_tag="ACHUN9_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0694509.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5310..6437)
                     /gene="rlmC"
                     /locus_tag="ACHUN9_001045"
     CDS             complement(5310..6437)
                     /gene="rlmC"
                     /locus_tag="ACHUN9_001045"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0694510.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6478..6966)
                     /locus_tag="ACHUN9_001046"
     CDS             complement(6478..6966)
                     /locus_tag="ACHUN9_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0694511.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(7026..7871)
                     /gene="potI"
                     /locus_tag="ACHUN9_001047"
     CDS             complement(7026..7871)
                     /gene="potI"
                     /locus_tag="ACHUN9_001047"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0694512.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7868..8821)
                     /gene="potH"
                     /locus_tag="ACHUN9_001048"
     CDS             complement(7868..8821)
                     /gene="potH"
                     /locus_tag="ACHUN9_001048"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0694513.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8831..9964)
                     /gene="potG"
                     /locus_tag="ACHUN9_001049"
     CDS             complement(8831..9964)
                     /gene="potG"
                     /locus_tag="ACHUN9_001049"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0694514.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLNGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10059..11171)
                     /gene="potF"
                     /locus_tag="ACHUN9_001050"
     CDS             complement(10059..11171)
                     /gene="potF"
                     /locus_tag="ACHUN9_001050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0694515.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11522..11998)
                     /locus_tag="ACHUN9_001051"
     CDS             complement(11522..11998)
                     /locus_tag="ACHUN9_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0694516.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12086..12988)
                     /gene="rimK"
                     /locus_tag="ACHUN9_001052"
     CDS             complement(12086..12988)
                     /gene="rimK"
                     /locus_tag="ACHUN9_001052"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0694517.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13049..13771)
                     /gene="nfsA"
                     /locus_tag="ACHUN9_001053"
     CDS             complement(13049..13771)
                     /gene="nfsA"
                     /locus_tag="ACHUN9_001053"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0694518.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13755..14042)
                     /locus_tag="ACHUN9_001054"
     CDS             complement(13755..14042)
                     /locus_tag="ACHUN9_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0694519.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14202..14459
                     /gene="grxA"
                     /locus_tag="ACHUN9_001055"
     CDS             14202..14459
                     /gene="grxA"
                     /locus_tag="ACHUN9_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0694520.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14489..14866)
                     /locus_tag="ACHUN9_001056"
     CDS             complement(14489..14866)
                     /locus_tag="ACHUN9_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0694521.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15136..16821
                     /locus_tag="ACHUN9_001057"
     CDS             15136..16821
                     /locus_tag="ACHUN9_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0694522.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16996..17532)
                     /gene="rcdA"
                     /locus_tag="ACHUN9_001058"
     CDS             complement(16996..17532)
                     /gene="rcdA"
                     /locus_tag="ACHUN9_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0694523.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17616..18824
                     /locus_tag="ACHUN9_001059"
     CDS             17616..18824
                     /locus_tag="ACHUN9_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694524.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18824..19639
                     /gene="ybjI"
                     /locus_tag="ACHUN9_001060"
     CDS             18824..19639
                     /gene="ybjI"
                     /locus_tag="ACHUN9_001060"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0694525.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19725..20009
                     /gene="ybjH"
                     /locus_tag="ACHUN9_001061"
     CDS             19725..20009
                     /gene="ybjH"
                     /locus_tag="ACHUN9_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0694526.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20050..21277)
                     /gene="mdfA"
                     /locus_tag="ACHUN9_001062"
                     /pseudo
     CDS             complement(20050..21277)
                     /gene="mdfA"
                     /locus_tag="ACHUN9_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21562..22158
                     /gene="ybjG"
                     /locus_tag="ACHUN9_001063"
     CDS             21562..22158
                     /gene="ybjG"
                     /locus_tag="ACHUN9_001063"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0694527.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22216..22974
                     /gene="deoR"
                     /locus_tag="ACHUN9_001064"
     CDS             22216..22974
                     /gene="deoR"
                     /locus_tag="ACHUN9_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0694528.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(23021..24223)
                     /gene="dacC"
                     /locus_tag="ACHUN9_001065"
     CDS             complement(23021..24223)
                     /gene="dacC"
                     /locus_tag="ACHUN9_001065"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0694529.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24470..25095
                     /gene="gstB"
                     /locus_tag="ACHUN9_001066"
                     /pseudo
     CDS             24470..25095
                     /gene="gstB"
                     /locus_tag="ACHUN9_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25092..26207)
                     /gene="yliI"
                     /locus_tag="ACHUN9_001067"
     CDS             complement(25092..26207)
                     /gene="yliI"
                     /locus_tag="ACHUN9_001067"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0694530.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26318..26701)
                     /gene="bssR"
                     /locus_tag="ACHUN9_001068"
     CDS             complement(26318..26701)
                     /gene="bssR"
                     /locus_tag="ACHUN9_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0694531.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26914..28239
                     /gene="rimO"
                     /locus_tag="ACHUN9_001069"
     CDS             26914..28239
                     /gene="rimO"
                     /locus_tag="ACHUN9_001069"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0694532.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28286..29607)
                     /locus_tag="ACHUN9_001070"
                     /pseudo
     CDS             complement(28286..29607)
                     /locus_tag="ACHUN9_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29615..>30911)
                     /locus_tag="ACHUN9_001071"
     CDS             complement(29615..>30911)
                     /locus_tag="ACHUN9_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR0694533.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7795 a         7969 c         7484 g         7663 t
ORIGIN      
        1 gcttaaattc tttacccaca attgtagcat aaataatatt atcgagagtc aaagctggga
       61 ctatcaaatt tcgctcattt tcaaaaatac aacgtaattt tggcaatcat cttaatcgca
      121 ggatctctta tcgcatgtga aaaattttgt aatgattaga caataaccgc gttcatttat
      181 cacgcgttat aaatgtgaaa ctggcttctt gcagatgctc ttaacctcta caggaggatt
      241 tataccacca tttcagaact atcatattta ccttaaatga tcatttcagc catataacac
      301 gctcaacctc acaaatggtt tacactgtct attgtttggg taatttaagt aagtcagcta
      361 cgagcaacct tgagtggata gccagcaatg gactcaaacc caatcaggtg ttcagattgt
      421 ttcagaattg tattaaaaac aatgtattaa ataatctgat agcgtttggt cgtagtgtga
      481 cgcagacagc aagctaataa ggaaatgatt atgcgctacc gttcactatc tctacttatt
      541 ccatgtgcac tgctgctgag tgcctgtacc accgtcacgc cagcttataa agataacggt
      601 ccccgcaccg gttcctgtgt ccaggggggg cctgacagcg tggctcaaca attctatgac
      661 taccgcattc aacatcgcag caatgacatt accgccctgc gcccctattt gagtgacaaa
      721 ctggcaaccc ttcttagcga tgccagccgc gataatagtc atcgtgaatt gttaagcagc
      781 gatccgttct ccagtcgaac caccttgcca gatagcgccc acgttgccag tgcatcgact
      841 atccctaatc gtgatgcccg taacattcca ttgcgcgtag atctcaaaca gggtgaccag
      901 ggttggcagg atgaagtgtt aatgattcag gaaggccagt gttgggtcat cgacgatgtg
      961 cgctacttag gcggcagcgt tcatgccacg gcgggtacgc tccgtcagtc catcgagaat
     1021 cgctaacgcc ttgatcaacc aataaacccc gtaaattgtc cggcatttcg tgaggaatgc
     1081 cgacatttat gctcgccgac caccgccccc gttattttgt gctatgttta ttgaataatg
     1141 cgctttgctt ttaactttta aagcagaaat attgcataat tattctgtca aaggtactat
     1201 cttcgacctc aatagctatc agactgccag tatacgagtg tcaatgagta ttcaattaaa
     1261 cggcattaat tgcttctacg gcgcgcatca ggcgctgttc gatatcacgc tggattgccc
     1321 acagggcgaa acgctggtgt tacttggccc cagcggcgcg ggtaaaagct cgctgctgcg
     1381 tgtactcaat ctgcttgaga tgccacgctc cggtacgctc aacattgcag gcaaccattt
     1441 cgatttcacc aaaacaccct ctgacaaagc gattcgcgat ttgcgtcgta acgttggcat
     1501 ggtgtttcag caatacaacc tgtggccgca tctgaccgtg cagcaaaacc tgattgaagc
     1561 gccctgccgt gtactggggt tgagtaaaga tcaggcgctg gcccgtgcag aaaaactgct
     1621 ggaacgtctg cgtctcaaac cttatagcga tcgttacccg ctgcatcttt ctggtggtca
     1681 gcagcagcgt gttgctattg cccgtgcgtt gatgatggaa ccgcaggtac tgctgttcga
     1741 tgaaccgacc gccgcactgg acccggaaat tacggcacaa atcgtcagca tcattcgtga
     1801 gctggcagaa acgaatatta cccaggtgat cgtcacccac gaagttgaag tggcgcgtaa
     1861 aaccgccagc cgagtggtgt atatggaaaa tggtcatatc gtagaacaag gcgacgcgag
     1921 ctgctttacc gagccgcaaa ccgaagcatt taaaaactat ctctctcact aagattcggg
     1981 ataacaacaa tgaaaaaagt tctgattgcc gcgttaattg caggttttag tctttccgcc
     2041 acagctgccg aaaccattcg ttttgctacc gaagcatcct atcctccgtt tgaatcgatt
     2101 gatgcaaaca accagatcgt tggttttgac gtcgacctgg cacaagcgct gtgtaaagag
     2161 attgatgcaa cctgcacttt ctctaaccag gcgtttgaca gcctgatccc aagcctgaaa
     2221 ttccgtcgcg tagaagccgt gatggcgggc atggatatca ctccggagcg tgaaaagcag
     2281 gtgctgttta ccaccccgta ctatgacaac tctgccctgt ttgtgggtca gcaaggcaaa
     2341 tacaccagtg ttgatcagct gaaaggcaaa aaagtcggcg tacagaacgg gacgacacac
     2401 cagaaattca ttatggataa gcacccggaa atcactaccg ttccgtatga cagctaccag
     2461 aacgcaaaac tggatctgca aaacgggcgt atcgacggcg tcttcggtga caccgcagtg
     2521 gtcactgagt ggctgaaaga taacccgaaa ctggcggcgg tgggcgacaa agtgaccgat
     2581 aaagattact tcggcactgg cctcggcatc gcggtacgtc agggcaacac tgagctgcag
     2641 cagaaactca acactgcgct ggaaaaagtg aagaaagatg gcacttacga aaccatctac
     2701 aacaaatggt tccagaagta attcctgatg aatgaatttt ttcctttagc aagcgccgcc
     2761 gggatgaccg tcggccttgc cgtttgtgca ttgattgtcg ggctggcgct ggcgatgttc
     2821 tttgcggtat gggagtcggc aaaatggcgt cctgtcgcgt gggcaggttc agcgctggta
     2881 accattctgc gtggcctgcc agaaattctg gtggtgctgt ttatctattt tggctcctcg
     2941 cagctgctgc tgacgctttc ggatggcttc actatcaatc ttgggttcgt gcagatccca
     3001 gtgcagatgg acattgagaa cttcgacgtt agcccgttcc tttgtggtgt catcgctctg
     3061 tcactgctgt atgccgccta tgcctcgcaa acgcttcggg gcgcgttgaa agcggtgccg
     3121 gtgggtcagt gggagtctgg tcaggcgctg gggctgtcga aatcggctat ctttttccgt
     3181 ctggtgatgc cgcagatgtg gcgtcatgcg ctgcctggcc tcggtaacca gtggctggtg
     3241 ctgctgaaag ataccgcgct ggtcagtttg attagtgtga atgatttaat gctgcaaaca
     3301 aaaagcatcg ctactcgtac ccaggaacca tttacctggt acattgtggc ggcggcgatt
     3361 tacctggtga tcaccctgct cagtcagtac attctcaaac gcattgacct gcgcgcgaca
     3421 cgttttgagc ggaggcccag ctaatgtttg agtatttacc cgaactgatg aaagggctac
     3481 acaccagcct gacgctaacc gttgcctcgc tgattgtggc actgattctg gcattgattt
     3541 ttaccatcat cctgacgctg aaaacgccgg tgctggtgtg gctggtgcgg ggttatatca
     3601 cgctgtttac cggtacgccg ctgctggtgc agatcttcct gatttattac gggccgggcc
     3661 agtttccgac gttgcaggag tatccggcac tgtggcattt gttgtcagaa ccgtggttat
     3721 gtgcgctgat tgcgttatcg ctgaacagtg cggcgtatac cacgcagctg ttttacggtg
     3781 caattcgtgc gatcccagaa ggtcagtggc agtcctgtag cgccctggga atgagcaaaa
     3841 aagatacgct ggcgctcctg ctgccgtatg cctttaaacg ctcgctctct tcttattcca
     3901 acgaagtggt gctggtgttc aaaagtacct ctctggctta caccattacg ctgatggaag
     3961 tgatgggata cagccagttg ttgtacggac gcacctacga tgtaatggtg ttcggtgcgg
     4021 cagggattat ttacctggtc gtcaacggcc tgctgacgct gatgatgcgt ctgatcgagc
     4081 gcaaagcact ggcattcgaa cggcggaatt aatgtagctg cgtacattcg ccgggggggt
     4141 ttgtaggtct gataagacgc gccagcgtcg catcagacat cagcacggtg cctgtgccgg
     4201 atgcggcgtg gacgccttat ccggcctaca tagtgcataa attcttataa tgaagacgga
     4261 caactcacta agttatccgt ctttttttat ttcatttaaa ttatttaatc atatttattg
     4321 catataaatt cacttgatgg cattgttatc ccatgccgca gacacggcca aaaatcataa
     4381 gattgacaga cgggagttcc atcatgaaaa agttagttct tgccgcttta cttgcttcct
     4441 ttactttcgg tgcttctgcc gcagagaaaa tcaattttgg cgtttcagcc acctatccac
     4501 cctttgaatc tataggtgct aataatgaga ttgtcggctt tgatatcgat ctggcaaaag
     4561 ccttgtgcaa acaaatgcag gcagaatgta cttttactaa tcacgcgttc gacagcctga
     4621 tcccgtcact gaaattcaga aaatatgact ccgtaatctc cggtatggat atcacgcccg
     4681 agcgtagcaa gcaggtatcg tttaccacgc cctactatga aaactcagcc gtcgtgattg
     4741 ccaaaaaaga tacctacaaa acgtttgccg atctgaaagg caaacgtatt gggatggaaa
     4801 acggcaccac gcaccagaaa tatattcagg atcagcaccc cgaagtgaaa actgtctcct
     4861 atgacagtta tcagaatgcc tttatcgatc tgaaaaatgg tcgtattgat ggggtatttg
     4921 gtgacacagc ggtggtaaac gaatggctga aaaccaatcc gcaactgggc gttgctactg
     4981 aaaaagtgac cgatccgcaa tacttcggca ccggtctggg catcgctgta cgtccggata
     5041 acaaagctct gctggaaaaa ctgaataacg cgctggcagc aattaaagct gacggtacat
     5101 atcagaaaat caatgaccag tggttcccgc agtaattgag actgatgaca aaagtaagat
     5161 tgcctgatgc gctgcgctta tctggcccac atcaactctg caatgtattg atttagcatg
     5221 attttgtagg ccggataagg cgtacacgcc gcatccggca tgaacaaagc gcacctgatc
     5281 agcaatctga agccgcacct gcggcttttt tattgcttca ccagcagcgt catcacttca
     5341 tagtgcgcgg tgtgcgggaa catatcaaaa agctgtaccc gttcgatgcg gtaacctggc
     5401 agttcgcgga tatctttcgc catggtttgg gcgttacagc tggagtagat aataaaacgc
     5461 ggtgccatcg ttgagagata atcacacagc ggtttaccaa tgccgcggcg cggcgggtta
     5521 accagcacca gctccggcac atccccctga gcggtggcaa actgagtgga gtccagcgcc
     5581 tgaaattgca aacgcgttaa gcccagttca gcggctgact gctttgcaca ggcaatggcc
     5641 tctgatgcaa tttcgatccc ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag
     5701 ccacccacac cgcagaacag atcccacata tgtttaactg gcagctgccg tacccagtcg
     5761 cgcgcggtag cgtacagctg gctggcgacc gccggattag tctggaagaa actttgcgga
     5821 cggatccaca gcggtacgtc attaaaacgc tctgccagcg cctgttgttc agtcaggtag
     5881 atctccgtct ccccttccat aatcgccata tgtaccggct gaatattgac ggtaataact
     5941 ttcagttgcg gtagttgttc ctgtaaccac ggcagcgcct tacgcagttg tgccagtttg
     6001 gtttcagaac gcagtacaaa gcgcagcatc atgccgccat cgctctggct ttcagtcagc
     6061 agaatgtatt tcagttcgcc acgcttacgc gccacgttgt agggcgttaa ccccgcacgg
     6121 gcgataaacg gttttagcgc cgcaaaaacg ggcgcaaaag aggcaggata aagcgggcag
     6181 tcacaaaggt cttccggcgt gccatcgcga tgcagcatac cgagcagtgg tttttcaacg
     6241 ctaccactca ccaccatttt ggctttatta cgaaaccctt gttccgggcc tgacaccggc
     6301 gcgcaccatt cctcaaccgg aaaatcggcg agcagatttt taagatcggc ggttttagcg
     6361 gagagttgct ctggaatcgg ctgcgttatc cactgacagg aacgacagcg acccgcgtcg
     6421 taaagtgcgc actgcataca ttgaccttca catcatcagg ggcgacgatt atacacatta
     6481 ttgcaactgg aagaaccgcc gactggtcga gggaacgaac agcagcatca ggatgagcat
     6541 atccggcagc ttctgcagca tcaggctatg gaagatttca cgttttgatt caccgggaat
     6601 gctgaacagc tccggataac cgtaccccag cgaagccgcc cacaagtaac ttgcggcggt
     6661 gatttgtgtc agcagatata gccagcgcgc ccagcgacgc cctttcacca gtgaaaacgc
     6721 acaccagatc tcaatgaaaa ccagcaccag actgcttaaa aagaccagcg ttaaactcca
     6781 ggtttgtacg ctgcgatgaa tgaactcgcc aataccgcgc acgcccagcg tattgaaaat
     6841 catcagcacg tcgaggccac ggatcataat aatggcgagc gccgccacct gcaccagcgc
     6901 aggcacattc aggcgagcat gagatgaaga tgttttctta aaaaatccca acgtttcgtc
     6961 ttccatgaaa acaatgccgc ggcatgcgcg gcattatgta gccaggttgg caaattttag
     7021 tgtcttcagc catgtcttgc acgctggata tcgcgtatcc gctgtttttc tgcgcgagcc
     7081 atcagatacc aggcgataaa tccgacaatt ccgaccgcac cgagaattaa cgttgcaagg
     7141 gcgttgattt ccggattcac tcccatccgc acgctggaaa agaccagcat cggtaacgtg
     7201 gtggctcccg gcccggaaac aaagctggcg atcaccagat catcaagcga caaagtaaaa
     7261 gccagtaacc agccagaaat gatcgcgggc atgatcatcg gtagcgtaat gacgaaaaac
     7321 actttcagcg gcgtcgcacc gagatccatc gctgcttctt ctatcgagcg atccagttcc
     7381 cgcaatcttg aagaaataac gaccgccaca taagccgtac aaaaagtgac atgcgccagc
     7441 cagatggtga gcataccgcg gtccgcaggc cagccaatgg catgagcaag cgcgacgaat
     7501 aataacaaca gcgacaagcc cgtgatgaca tctggcatca ccagcggcgc ggtgatcata
     7561 aaggcaaaac catttgatcc gcgaaacctg ccaaaacgca ccagcaccac cgccgcaata
     7621 gtcccgagga tcgccgccgc cgttgccgca caggcagcaa ttgtcaggct taaaccaacc
     7681 gcactcatca tcgcgtcatc gcgcaataac tcaccatacc agcgcgttga ccagccggcc
     7741 cacaccgtca ccagcttcga gctgttaaac gaatagataa ccagcatcag cattggcgcg
     7801 tagagaaagg taaagcccag cagcaaaatc acaatccgcc agggcgaacg aactaccggt
     7861 aaattattca tccgtgttct cccacgcttt tttgctggtg tttgtggaac cacattatcg
     7921 gcacaattag cagcagcaac atgatgatcg ctaccgccga ggccaccggc cagtcgcggt
     7981 tattgaaaaa ctcttgccat aacacgcgcc cgatcatgat gctgtccggg ccaccgagca
     8041 gttccgggat cacaaactcg cccacagccg ggataaacac cagcatcgat ccggcaataa
     8101 tcccaccttt agtcagcggc acgatcacag taaagaacgt tttcagcggt cgtgcaccga
     8161 gatccagcgc tgcttccacc agcgaataat caatacgaat caacgcggta taaatcggca
     8221 gtaccataaa cggcacgtaa gcgtaaacaa tgccgatata aacggccaga ttggtatgca
     8281 gaatggtcag cggttgatcg ataaccccca gccacagcag aaaattattc agcacaccgt
     8341 tgttttttaa tattcccatc caggcataaa cgcggatcag aaacgaggtc cacgacggca
     8401 ggatcaccag tagtaataaa atattacggg tcgaaggctt actgtgcgcc accgcccacg
     8461 ccagcggata gccgatcagt aaacagcaaa atgtcgaaat cgccgccacc tgtaacgact
     8521 ggagataagc atcaaaatag agcggatcgt cggtcagttg cagaaaatta ccgagattaa
     8581 gagtgatgga aagttgcccg tcagcccact ccatcagttc ggtataaggt ggaatagcgc
     8641 gcgccatctc tcccaggctt attttaaaga caatcagaaa tggcagcaga aacagcaaga
     8701 tcaaccagat atatagcaac gcaatgacca gtttgcgccc atgcttcatt tgcagctgcg
     8761 acaaccacag cttaaatccg cccggcggtt tcgactgggc agcaggttca agtgtactca
     8821 tctttgctcc ttaaaccgtc agcaccacac agctgtccac ttcccagcac aaacgcactt
     8881 cgtcgcccca ggtcggtaac cctttacgat gacgatgggc gttttgtagc tgggcgctga
     8941 tcatctgccc acttttcagt cgaacgtgat acaccgaaag atcgccgaga taggcaatgt
     9001 gtatcacctc ccccaccgcg aagttacaac cattggcggg cggctcttcg caaagcatga
     9061 ttttttccgg gcgcagcgct acgtgtaccg gcacgttatc gaccactgaa gcatccgcat
     9121 cgactttcag tggatgcacc agccccggcg aatcaagcac caggccatct tcctgacgct
     9181 ctttgagtac accttcaaag acattaaccg aaccgataaa ttcagcgctg tagcgggtag
     9241 ttggatgctc gtagatctct tccggctcgc caatctggac aaatttcccg cgattcataa
     9301 tggcgatacg ccccgccatg gtcatcgcct cttcctgatc gtgggtgacc atcacacaag
     9361 tcacaccgac gcgctccaga atatccacca cttcaagctg catccggtca cgcagctttt
     9421 tatccagcgc gcccatcggc tcatcgagca gtaatagttt cgggcgtttg gcaaggcttc
     9481 gggccagggc cacacgctgt cgctgaccac cggaaagctg atgcggtttg cgtttggcga
     9541 actcctgcat gtgcaccagg ccgagcatct catggacccg actggcaatt tccgctttcg
     9601 gtagtttgtc ctgtttcagg ccaaaagcga tgttctgttc cacggtcata tgcggaaaca
     9661 gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta aggcggcacc tgtgacaaat
     9721 cgacgccatt gagcattatc tgcccggcag aaggttgttc aaaacctgcc agcatacgca
     9781 gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc gaagatttca cctttgtaga
     9841 tggtcaggct gacatcatcg accgcatgct gaccatcgta ggatttggtg aggttgcgga
     9901 tttctaatag cggcgtcagc gccttacggg ttttcgcctg cgggcgaggg atagcgtcat
     9961 tcacggctgc tctccggcat tgaatgtgtg caagcgcacc atgatggtgc gaattgccgg
    10021 ctggtgtggt gcgcccgccc ggcatctaag gctgcggatt attttccgct cttcactttg
    10081 gtccacgcgc gggtgcgcac acggtcgatt ttcggatcct gtactttcag agtgaatagc
    10141 ttcgcacgaa cgtccgccgg aggataaata cccgggttct cacggacttc cgcactcacc
    10201 agcggcgtgg ctgctttatt ggcgttggca tagaacacat ggtcggaaat atgcgctact
    10261 acatccgggc gcagcaggta attcaggaac tgataggctt cgtctttgtt tttggcatcc
    10321 gcaggcatgg cgaatacatc aaagaacgcc atcgcccctt cttttggaat cgagaacgag
    10381 acattcactc cattcttcgc ttccttcgcg cggtttgacg cctgccagac atcacctgcc
    10441 cagccgatag cgacgcaaat atcgccgttt gccaggtcgt taatgtattg agatgaatgg
    10501 aaataacgaa tgttcgggcg cagctttaac agcagatcgg ttgccggtcc ggtgtaatca
    10561 tccgctttag tgctgttggg atctttgccg agataattca acacggtagc aaaaacttct
    10621 tctggcgcat ccaggaaaga gacaccgcag cttttcagtt tttccagatt ttcaggtttg
    10681 aggatcaagt cccagctatc gacgggcgcg ttttcgccca gcacagcttt aactttatca
    10741 acgttatagc caatcccggt cgtcgcccac atatagggca tagcaaattt attgtcggga
    10801 tcgtgtttgg cgaccagctt cagcagctcc gggtcgagat tcttccactc cggcaatttg
    10861 cttttgtcca gcggctggaa aactcccgca gtcaactggc gttcaagaaa gctggcagat
    10921 ggaaccacca agtcaaagcc ggtgctaccc gccattaatt tgccttccag tacttcgtta
    10981 gagtcgaaaa catcgtagac gactttaata ccggtttctt tttcaaaatt ggccaccgtg
    11041 tccggggcga tataatcaga ccagttataa atgtggagtg ttttttgttc agccgcgagc
    11101 gtgccgacag agacggccat cagagcaccc gcagccagac ccgaaagcca ttttttattt
    11161 aaggcggtca tatctctttc cttctgaaag ttcgttaaca aacgaacaaa agttgtcaca
    11221 ctaaaagata tgcaataaac gtgcatattt tttatcggcg gggtgtaaga aaagagaact
    11281 ctctcccggc cagcactgct cgctatataa aagcatcgca agaataactt tagccagggt
    11341 ttgagactat agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct
    11401 atgtgataaa gccaaatcga cacaagagaa gtgaaataat ctttaagaaa aggttaaaaa
    11461 cagcagataa ttttgcagcg cgcagccact gcaccagcga ctgcgtgctc gaaaggagtt
    11521 atcagtggag gaaatagttt tcggtaacgt tgttttgccc ttcatcgtca gtcggtaaca
    11581 gcagttgatg ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca
    11641 cgaaccatgc aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca
    11701 ccagctttgg cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg
    11761 cattgatggc agataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca
    11821 tggcagaaaa caggatcgtg ttatcgatca gatcgatttt ggcatcaaag acaccgtcga
    11881 aattctgcat atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc
    11941 ccaggtcatc gagccattga cgcagcgtat ccagaccagg aacgaccagc gatgtcataa
    12001 ttttgtacaa cctctttcga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa
    12061 accatgatca gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag
    12121 cgtggcgttc gatccagcgg atcattttac ccgcgatgtc gataccagtg gttttttcta
    12181 ttccttccag ccccggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca
    12241 gaatatccac accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac
    12301 gctcctgtgg tgtgatactg gcgacgcttg ccgcgccgcc acgatgcaaa ttggaacgaa
    12361 aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca acaaccagac
    12421 agcggatatc gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca
    12481 gaccgcggaa agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc agcacgacgc
    12541 caattccctg cgtgccttca accaacttga ccaccagcgg cgcaccaccg accatgtcga
    12601 ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct
    12661 gacgcgccag cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact
    12721 cattgagcgg atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg
    12781 taatggcggt gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt
    12841 agtgaataga agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt
    12901 caaccaggtg accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc
    12961 catcccggga caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc
    13021 ccgcgtatca tacatcgacg tggcggtttt agcgcgtcgc ccaaccctgt ttgtgcaaat
    13081 aatccagaat aaatgggcgg ctttctttaa tgattgttcg gcggatatga tcgctccagg
    13141 tatcccggcg attattgctg ccacgggtga ggtaatattc cgccagttgc tcgtcatact
    13201 gcgccagtgc gtctttatcc agcggttgat agctgttttc atgcaccaaa atggaggccg
    13261 gtaaacgcgg cttgagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg
    13321 gcagaacatg ctgcggtaat ttcagcagtt tcgtcaccgc ttcaatatta ttgcgcaggc
    13381 cgccgatata taccccgccc aatcccagcg attccgctgc ggttaatgca ttctgcgcca
    13441 tcattgccgt atcaacgaca ccgagcaaca gttgttccgc caggccgagc tgagcatccg
    13501 gacagatctg taaatggcgg ttaaagtcgg cacagaacac ccagaactcc gccgcttgcg
    13561 ctacgtgttt ttgcccgccg gtcagcgtca ccagttcttc acgtaatgct ttgtcggtaa
    13621 tgcgaataat gctactacac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa
    13681 taatcgcctc acactgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat
    13741 ggccacaaat aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat
    13801 tgcggtggac ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc
    13861 accgcagcgg gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc
    13921 cgagaaaatg gctcaggcag cgacagggag tcgcttaccg acagcagcgc caccgccagt
    13981 agcatcattc cgataaattc cagtatcaac acgcctttag gcaatttacc gatcgcgcgc
    14041 atacgcttcc ctctgcaaag tgagccttca gtctaaaact tttcactgta ttgtgtttaa
    14101 cagttatagc ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt
    14161 agcgtgatca ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg
    14221 gtcgttcggg ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg
    14281 aacgcgatga ttttcagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag
    14341 atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc
    14401 aacatatcgg cggctatacc gattttgctg catgggtgaa agaaaatctg gacgcctgat
    14461 cgtctgacaa gccctcgcgt tgagggcttt actgattttt tctgtgctgt ggtttaaaca
    14521 aactactgat aaataagaaa cacagtgccc ccagcgcaca ccagaacacc gcgcttagta
    14581 accatgccag ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc ataatgagca
    14641 ttgaacaggg tgccgccagc attgcgccaa atagaggttt caggacttct ctacgctgtg
    14701 aaaagaaact ggcaactgct ccaggaagaa tgaaaaacag caagccgatt tcaggatgcc
    14761 cggcagcccg aaaagcgcct ttcatgtgcg tcgccagaaa aaggcacacc acaatgaaga
    14821 ggacaaaaca gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac
    14881 actgcgtcta tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg
    14941 cattccatgc taatataggc caacgcaatt catatagccg ttgataccta atgtgattac
    15001 actagtaaaa tatattgtta ctttactatc gtttaggtgc gctgaatgaa tctgcgccct
    15061 gaattctggt aaaaaacatt atcgtaaatt accatttctt tcaacagctt actagtaaac
    15121 aagaagttag cctccgtgaa tataaacgtc gccgaattgt taaatgggaa ttacattctg
    15181 ttattatttg tggtcctcgc gcttgggcta tgtctcggaa agttacgact tggttcgatc
    15241 caactgggta attccattgg cgttttagtc gtatcgctgt tattaggcca acaacatttc
    15301 agcattaaca ccgatgcgct taatcttggc tttatgctgt ttattttctg cgtcggggtc
    15361 gaagccggac cgaacttttt ttccattttt tttcgcgatg ggaaaaatta cctaatgtta
    15421 gcactggtga tggttggcag tgcgctggtg atcgccttag ggttaggtaa gctgtttggc
    15481 tgggatattg gcctgacggc cggtatgtta gcaggctcta tgacgtcgac accggttctg
    15541 gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa gcaggcagct ctcactggca
    15601 ctggataatc tgagcctcgg gtatgcctta acctatttaa tcggtctggt gagtttgatt
    15661 gttggtgcgc gttacttgcc gaaattgcag catcaggact tacagaccag cgcccagcaa
    15721 atcgcccgcg aacgtggcct ggacactgat gccaaccgta aggtttattt accggtgatc
    15781 cgcgcctatc gcgtcggccc ggagctggtg gcctggaccg acggcaaaaa tctgcgtgaa
    15841 ctgggtattt atcgacaaac cggctgctac attgaacgta ttcgacgtaa cgggattctg
    15901 gcaaatccag acggtgatgc cgtgctacaa atgggcgatg aaatagcgtt ggtaggctat
    15961 cccgacgccc atgcccgact cgatcccagc ttccgtaacg gtaaagaagt tttcgatcgt
    16021 gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca taacgctgta
    16081 ggtaaacgtc tcgcacaact gaagttgacc gatcacggtt gcttccttaa ccgcgtcatt
    16141 cgtagccaga ttgagatgcc gatagatgac aacgtcgtgc ttaacaaagg tgacgtttta
    16201 caagtcagcg gtgatgcccg tcgcgtaaaa accatcgccg atcgcatcgg ctttatctcg
    16261 attcacagcc aggtcactga cctgctggca ttttgcgcct tctttgttat tgggctgatg
    16321 atcgggatga tcaccttcca gttcagcaca ttcagtttcg gcatggggaa cgctgccggg
    16381 ttgttattcg ccggaattat gctgggcttt atgcgtgcta accacccgac cttcggttac
    16441 attccgcaag gtgcattaag catggtgaaa gagttcggct tgatggtgtt tatggcaggc
    16501 gttggtctga gcgccggtag cggtattaat aacggcctgg gcgcgattgg cggtcagatg
    16561 ttgattgccg gattaattgt cagtcttgtg cccgtggtta tctgtttctt gttcggtgct
    16621 tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc
    16681 gcgccggcaa tggagatcat cagtgataca gctcgcagta acatccctgc gctgggctat
    16741 gcgggcacct acgcaatcgc caacgtcctg ctgacgctgg cagggacaat catcgtcatg
    16801 gtatggccag gattaggata aaactgaagt tgccctgaaa atgaaatttt tttgcacaac
    16861 cgcagaactt ttccgcaggg catcagtctt aattagtgcc actgcttttc tttgatgtcc
    16921 ccattttgtg gagcccatca accccgccat ttcggttcaa ggttgatggg ttttttgtta
    16981 tctaaaattt atctactacc ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga
    17041 tagcggctta cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc
    17101 gcgggcggtt ccgggttcaa accattgttc gagcgtttgc tgactgcgct gcatccagtt
    17161 ttgcattacc gtttttaata gcggttttcg gctagccagc gcgtagagct ggtacatcag
    17221 ctccatatta tccggcgttg caacctgtga gctgtagatc atatcggtga tagcctggca
    17281 tgcgccttga gcatcactaa catcgctaaa aaatgcctga tattgccggg acataatctc
    17341 agtaaaactg ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat
    17401 cgatcccaac ggtaccccgg caagggtagc aattttgcgg tgcgtaacag catgtattcc
    17461 gtaaagtttc accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt
    17521 agcgcgacgc atgtcatttc ctctgtctgc aattgtgtac aaatgtacac aaccttgcta
    17581 atgttgtctt ccctcttctt attttgacgt gatctatgac cgtaaattct tcacgtaatg
    17641 cattgaaacg ccgaacctgg gcgctgttta tgttcttctt tttgccaggc ctgttaatgg
    17701 cgtcctgggc aacccgtacg cctgctatcc gcgatattct ctctgtctcg atcgctgaaa
    17761 tgggcggtgt tctctttggt ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt
    17821 ggttagtgaa acgctttggg acacgtaatg tcatcctggt cacgatgtcc tgcgcattga
    17881 tcgggatgat gatattaagt ctggcactct ggctgacatc gcccctgctc tttgccgttg
    17941 gtctcggcgt ctttggggca agttttggtt ctgcggaagt ggcgataaac gttgaaggtg
    18001 ccgccgttga gcgagaaatg aataaaacgg ttttgccgat gatgcacggt ttttatagcc
    18061 taggcacgct ggcaggcgct ggtgtcggga tggcactgac ggcctttggc gttccggcaa
    18121 cggtgcacat tttattggcg gcgctggtag gtatcgcgcc tatttatatc gccattcagg
    18181 caatccctga cggtacgggc aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca
    18241 ttccttttta tcgcgatatc cagttgctgt tgattggtgt tgtagtgctg gcgatggcct
    18301 ttgccgaagg ttctgccaac gactggttac ccttattaat ggttgacggt cacggtttta
    18361 gccctacttc cggctcgctg atttatgccg gttttaccct agggatgacc gttggacgct
    18421 tcactggcgg ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac
    18481 taatgggggc gttgggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg
    18541 tgtctgttgt actctgggga ctgggtgcct cgctgggctt cccgctgacc atttctgccg
    18601 ccagcgatac cggccccgat gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc
    18661 tggctttcct cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta
    18721 gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg
    18781 ccaaacccga taccaaaacg cggacggcga tggagaatag ttgatgagca ttaaattaat
    18841 tgcggtagac atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt
    18901 tatggctcag tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa
    18961 tcaatattat cagttgctct ctttcttccc tgaaattgct aatgaaattg cctttgtggc
    19021 tgaaaacggc ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa
    19081 ggatgccttt actactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc
    19141 ctgcggaaaa aatagcgcct atacgctcaa aaaatatgac gatgccatga aaacggtggc
    19201 agaaatgtat tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt
    19261 ctttaagttt ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca
    19321 tgaggccatc ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat
    19381 tatccccggc gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga
    19441 cgacagcgaa gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc
    19501 aggctttagt tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg
    19561 ggcaggctcc aataaccgtg aaggcgtact ggatgtgatc gataaagtgc ttaaacacga
    19621 agcgccattt gaccaataaa aacggcagca cgcccggcaa cacaatacct gaatgaaggc
    19681 tgcataattc gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt
    19741 gtctactgtt gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca
    19801 ccggcgatgt gccgagtgga tacaaagtgg ttgtagttcc tgattcagtc agcgttccac
    19861 aagcggtcag cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg
    19921 cttaccgccc gcatccttat gctcgccatc ttgccagcgt aggtgaaggg atggttatcg
    19981 aacaccagat tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcagtca
    20041 ggcatttttt tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat
    20101 cagcgacagc cacaaaattc cgttgacaag gttgaagaga ttaaactgtc cgttgccccc
    20161 gttcagccag gcatgtttgc tgatttcaat accaacggta aagatcagca tttgcagcat
    20221 tcccatcgcc gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac
    20281 cagtcccgca ttcgccagac caataccgaa agcataaata cttaacccgg cagtcatcca
    20341 taaatacgcg tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat
    20401 cggccagccg cccataatta tcacgtacgg tgcggcgcga ggtcagacgc gctaacagca
    20461 agttacccgc aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc
    20521 tcaactgctc cccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca
    20581 ggctaacgaa tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct
    20641 tatagtcacg accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag
    20701 gcatagctcg ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aaaacaaaca
    20761 tcccctccca gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag
    20821 caatcagcgc cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg
    20881 attcctgaat tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga
    20941 agcgcaacag ggtgaattgt tcaatgtttt gcgccagtaa tattgccaga caggtgacga
    21001 taaaccacac cacacccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc
    21061 ccaggagcca ttgtaaaaac atcccgcccg ccagatacgc ggtcatcgaa gtaggaaccc
    21121 aatcaatgcc cgcctgatat tgttccacca cggccaacat accgggttgg atcatatcgt
    21181 tgccgatata ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac
    21241 gtccaagcct ggcaccgaaa gctaatttat tttgcatgca atttcttcgc caataataat
    21301 cgcgcagagt ttaataaaag cgcagctaac gagaaagcga attttgtagc tgaaaccacg
    21361 gttaagcaca ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac
    21421 ctatgcccgt tacaatcacc caccgtaaac aggccgcttg agggaaataa gacgatgccg
    21481 ctttacccag tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta
    21541 atcactttgc gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt
    21601 aacgcgacgc cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat
    21661 ttgattaccg tggtgccgtt gctggccgcg gtactttggt tgtgggggct tacagcacaa
    21721 cggcaactgg tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg
    21781 atgggacatc tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg
    21841 catcatgcgg cggatgactc attcccgagc gatcacggta cggtgatttt cacctttgca
    21901 ctggcatttt tatgctggca tcgcctgtgg tccggctcac ttttaatggt tctggccgtc
    21961 gtcattgcct ggtcgcgcgt ttatcttggc gtccactggc cgctggatat gctcggtgga
    22021 ttgctggcag gtatgattgg ctgccttagt gcccagatta tctggcaagc gatggggcat
    22081 aaactctatc aacgtctgca atcgtggtat cgcgtctgtt ttgcattacc gatccgcaaa
    22141 ggctgggtgc gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa
    22201 ctattcagag ggattatgga aacacgtcgc gaagagcgta tcgggcagct gctgcaagaa
    22261 ttaaaacgca gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag
    22321 atgacgattc gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat
    22381 attgttctgg aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg
    22441 gtggaagaaa aacgccgggc ggcaaaactg gctgcgacac tggtagaacc cgatcagacc
    22501 ctcttttttg actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc
    22561 ccttttaccg ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat
    22621 tgccgcgcat ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaccccatc
    22681 gattttcagc aaacgttgaa taatttttgc ccggatatcg ctttttattc cgcggcgggc
    22741 gtgcatgtca gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg
    22801 gccatgtcga tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg
    22861 cgtccggcgc gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg
    22921 gaagatgagt atgtgaagta cgcgcagacg caccgtatta agttgatgta ttaatgacgt
    22981 ataaccggat gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga
    23041 accactgatg gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt
    23101 ccacattttc catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat
    23161 caatggtccc gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg
    23221 tgtagctcgc cttcaggttt ttcagctgcc cacgcggtat ggtcacggag cccgcttcgc
    23281 ctgccccgag attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg
    23341 catcaggttt aattggcgtc acagtttcaa agaagcggaa accccaggtc aataatttct
    23401 cagactcatt aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca
    23461 tatcaccctg ggtagccgaa gcaaccagat tatatcccgc acctgccgtg gttcctgtct
    23521 tcatgccatc aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa
    23581 ttttgttgaa ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga
    23641 tcaatgcttt acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca
    23701 gaccgtgcac cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca
    23761 tcaaaccaat aaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt
    23821 cattaccgga ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg
    23881 gtttgaggaa cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt
    23941 taccgaccgt caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca
    24001 catagctggt catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt
    24061 ctgccagcac tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg
    24121 gcgcttcaac ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag
    24181 aacccgctgc aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat
    24241 ccattcaaaa aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac
    24301 acaaacttaa agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc
    24361 ttcgctgctt ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac
    24421 catcggagtc ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct
    24481 gtggggtcgg aataattcaa ctaacgtaaa aaagtattgc tgacgctcga agaactggaa
    24541 ctaccttatg agcaaattct cgcgggccgt gagtttggga taaatcacga tgctgatttt
    24601 ctggcgatga accctaacgg gctggtgccg ttgttgcgtg acgacgaaag tgatctcatt
    24661 ctttgggaat caaacgccat tgtccgctat ctggcggcac agtacgggca aaaacgcctg
    24721 tggatcgact caccggcacg tcgtgcggaa gcggaaaaat ggatggactg ggcaaaccag
    24781 acgctcagta atgctcatcg cgggatcctg atgggattag tcagaacacc accggaagag
    24841 cgcgatcagg ccgccattga tgccagttgc aaagagtgcg acgccctgtt tgccctgctc
    24901 gatgcggaac tggcaaaagt aaaatggttc tctggcgacg agtttggtgt gggcgatatc
    24961 gctatcgcac cgttcatcta caatttgttt aacgttggcc tgacctggac accgcgtcca
    25021 aatctgcaac gctggtatca gcaactcact gaacgccccg cagtgcgcaa agtggtgatg
    25081 attcccgtta gctaattgcg tgggctaact ttaagtaatt ccccactgga ctcgtcggtg
    25141 agaacgtata aataaccgtc gggtccagtg cgaacatcac gaattcgctg ccctctgtcc
    25201 gttaaaatac ggccatcttc tgtcactttg tcgccgttga cgctcatcac aatgacatct
    25261 ttatctttca gcgcgccaat aaataatttt tgctgccact gggggaattt atcgctgtta
    25321 tagaaggcca tgccgctcac agcgggcgaa tctttccagt aaaaaacagg ttgctcggtc
    25381 ccggcgacga tctccccttt cgcttccggt atcttaaagc ctgaatagtt gattccccag
    25441 gttgccagcg gccagccgta gtttttgcct ttttgcggga tattaatttc atcaccaccg
    25501 cgcgggccat gttcattcag ccacagtgca ttactccacg gattcatcgc cattccttgc
    25561 ggattacgaa tgccataaga ccagatctcg gcgcgcgcac cggattcctt tataaaagga
    25621 ttatcatccg ggatttcgcc ctggtcggtc agacgcacca gtttgccctg taatttatcc
    25681 agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag caataaaaag ataaccttta
    25741 ccgtcgaata ccagccgccc gccaaaatgg ttgccggtag acagttttgg catctggcgg
    25801 aagacggtgc ggaagtcggt cacttttgag agatcatcac ttaagcggcc ataacccaca
    25861 gcagttccgg ctttgccatc atcgccaact tcggaataac ttaaccagat gcggcgagac
    25921 tgagcaaaat caggcgctaa aaccacgtcc agcaggccgc cctgcccgtg cgcccaaacg
    25981 tccggaactc cggaaagcgg cgcagataat ccttttcctg cttgccagtg acgcaactcg
    26041 ccgccgcgca gagtgattaa cataccgtga ttatcgggta aaaaggccag tgcccaggga
    26101 tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg caggagccgc ccagagagcg
    26161 gaagaaagac aaataagggg cacaaggaaa aaggattgtc gatgcataac gcctccggat
    26221 tgactctggc ttaaagcgta gtcagtggag gagataacaa attcattttt acaaaaactt
    26281 aaacatgaag ggggagacgc tttctccccc ttagttttca ggccttctca agcatggcgt
    26341 gcttctgcag gctctggata ctcagcgtta agctcatcag acaattttca agcttatcgg
    26401 cgttgacggt aataacagtc gggcaatcat ggtgcccact catcaaacat actgcggctg
    26461 tcgctaatgc ttcttcagca tgatgaagag cactccactc ttcctgatcc agatgaagat
    26521 tcaaccgcag cgatttatcg tgcagttcgc gattcagttt aaaaaagtta tctcgtagat
    26581 gattgctttc gctgacggac atgtatcctt ttgccttcct cagttgcacg tatgcagcga
    26641 ggtcaacgcg tgcgtcgatc aaccggttca gcagatcgat tcgctgtctg tcaacgaaca
    26701 tacttcgatc ctcctctttc cagccttgtt gcgattaaat agtatggcta ttttttgagc
    26761 aaaatgctgt ccaggatcaa ttcttcacgc caaattaaca aaatttacat ttccttcgcc
    26821 tccgccagcg aatgagattg cgatacaaat tttatccaga actctgtaga atccctgccc
    26881 tgacctttcc tgtaactgaa tattttttaa gctatgagca aagtaactcc ccagccgaaa
    26941 atcggctttg tttcccttgg ctgtccgaaa aaccttgtcg attcagagcg tattctcacc
    27001 gaactacgca ctgaaggtta tgacgtggta ccgagctatg acgatgcgga catggtgatc
    27061 gtcaacacct gcggctttat tgacagcgcg gtacaagaat cactggaagc cattggtgaa
    27121 gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc tgggggcaaa agaagatcag
    27181 atccgcgaag tccacccgaa agtgctggaa atcaccgggc ctcatagcta tgagcaggtt
    27241 ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca acccattcct gagcctggtg
    27301 ccagaacaag gtgtgaaact gacaccgcgt cattatgcct atctgaaaat ttcggaaggc
    27361 tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc gcggcgacct ggtgagccgt
    27421 ccgattggcg aagtgttaag tgaagcgaaa cgtctggtag atgcgggcgt taaagagatt
    27481 ctggtgatct cgcaggatac ttccgcctat ggcgttgatg ttaaacatcg tactggcttc
    27541 cacaacggcg agccggtaaa aaccagcatg gtcagcctgt gcgaacagtt atcgaaactg
    27601 gggatctgga cacgtctgca ctacgtttac ccttatccgc atgtggacga cgtcatccca
    27661 ctgatggcag aaggcaaaat cctgccgtat ctggacattc cgttgcagca cgccagcccg
    27721 cgcattctca aactgatgaa gcgtccgggt tctgtagatc gccaactggc gcgcatcaaa
    27781 cagtggcgcg aaatctgccc ggaactgacc ctacgctcaa cctttattgt cggcttccct
    27841 agcgagacgg aagaagattt ccagatgcta ctcgacttcc tgaaagaagc gcgtctggat
    27901 cgcgttggct gctttaaata cagcccggtt gaaggtgcag acgccaatgc cctgcctgac
    27961 caggttccgg aagaagtgaa agaagaacgc tggaaccgtt tcatgcagct gcagcagcag
    28021 atttccgccg agcgcctgca agagaaagtg ggccgtgaaa ttctggtgat tatcgacgaa
    28081 gtggacgaag aaggcgcgat tggtcgcagc atggcagatg caccggaaat cgacggcgcg
    28141 gtttatctca atggtgaaac caacgttaag ccgggtgata tcctgcgtgt gaaagtcgag
    28201 cacgccgatg agtacgattt gtggggtagc cgggtttaat taattcaggc cgggcaaaat
    28261 catttgtccg ccctgcgaat aatgattact gccagctaat ctgagccctg ccgtttttct
    28321 tactcttata cagccgctca tccgccagca ttttcaagcg ttcgacggtg gtacattcat
    28381 ttgaatgcgc tacaccgata ctggtactta aattaatgct tcccacctgg aaatgaacga
    28441 tttcttttag aatcgatagc aactcttcca gcgttccacc cgtataaatc accgcaaatt
    28501 cgtcaccgcc gaagcggtag attttacctt tatctaccgg gatctgtgat ttaaggattt
    28561 tcaccacttt catcaacact tcatcgccca gcaaatgacc gaaggtgtcg ttaatgtttt
    28621 tgaatttatc gacgtcaaac aggcacaaat aaccgccttt ttccggcagc gcttttaaat
    28681 cttcgtcaaa ggattttctg cccattgctt tggtcaggtc gtccaggaac gccgagttat
    28741 acatactgta gagttttttg acatcggcag ccgaaaagaa aagcccgcca tccagtacgc
    28801 gttcaaaatg gttattaatg atggcttctt tctccacaat accacgatta atcactctgc
    28861 gtaccagata gaggcaactg gtacccgata tcactgccac caatgcggca aagaaaataa
    28921 tcaatgtaca gacccgcaaa taaacatctt ttttatacgc aaatacgtac aatccggtcg
    28981 gcaaataggc gaagtgctcc atcgcttccg aaacgatgaa gatggcgttt ttagcattat
    29041 attcatcacc ggtttaccgt tcttaaatag agagaacatc aggttctcgc tgtccatcgc
    29101 cagcgatttt gacagtaaag aaaaacgctc cagaccaagc aaaaatatca gactacgatt
    29161 gtgagttacc ggatagaggt aatagacgaa ggttttaccg tcaaaagaga taaaccctgt
    29221 atttttgaca tgtcgatact tcacaacctg cataatatct gcacaataat ttttctcgaa
    29281 ctcctttacc tgaatacatt cgtcactgga aatcagataa ggaccgacat cgtacaaata
    29341 agagggggaa accgagtcgg cgtaagctgt gcgcaaacca accaacagct gttcctccgt
    29401 gggatttagc gtgttgataa gatcaattgc cacgctacgc cgatattcag caatatttgc
    29461 caccgcaaaa gcagtatcaa taacggactg tttcaggcta tacacccgtt cctttaccat
    29521 ttgagtgtag atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt
    29581 aagtacgaac ttattgattc tggacatacg taaactactc ttttactaat tttccacttt
    29641 tatcccaggc ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc
    29701 ctaatttaat taattcgcgc gcaacgtctt cgctctctac gccttccgca atcaccggga
    29761 tccggttata gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa
    29821 tatcgccggt aaattgcgag tccaccttta tgctgtctgg attaatttca taaaaacgtt
    29881 tcaggtttga caaaccggtg ccaaagtcat caatcgatat ccgtacccct tgtttacgca
    29941 gttgttcaat attgagataa aaatgttcat taattcgctc gatcacggtt tcagtgatct
    30001 caacacagag gctgttcgcc ttcaggccat tttctttcat gatggtggta aaacgctcat
    30061 gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaatcatcct
    30121 tcagcgacgc acgctgacta aaatgtctga attcacccat tgccgtttta ataatctgat
    30181 aacccagctc attgataaac ccgatatctt cagcaatggt aatgaacttc agcggtgaaa
    30241 tgatcccccg ctccgcagaa acccaacggc acaacgcctc cgcgcccaga atggcgcggg
    30301 tgttaatatc aacgatcggt tgatagacga ggaatagttc gccaatttcc attgcatggc
    30361 gaatattacg cgcaatccag atattctctt ctatttcgtt gagtttcgcg tcatcacaga
    30421 aaataaactc ggagaacata tggtcacgga tactggaaat cgccagtttc agtttttctg
    30481 cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat ggcagactta tggatgcgca
    30541 gattgcggta gccaaagctg ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg
    30601 tctgtacatt ttcccgacac agaagtaaat agagatcggc attgaatgtc acgatatcgc
    30661 agcattgcgc atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt
    30721 catcaacacc aaacaccgct tccacggctt ccagaccaca cagtttgatg atccccacag
    30781 ccagatactg attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct
    30841 gatcgtaaga gatcagtgag agcaatttat ttgaccgttc aatttcggca tctttcattt
    30901 ccgcgatcga a
//
LOCUS       DAZOYS010000026        30154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOYS010000026 DAZOYS010000000
VERSION     DAZOYS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.026"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUN9_001072"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUN9_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0694534.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUN9_001073"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUN9_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0694535.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUN9_001074"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUN9_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0694536.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUN9_001075"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUN9_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0694537.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUN9_001076"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUN9_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0694538.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUN9_001077"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUN9_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0694539.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUN9_001078"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUN9_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0694540.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUN9_001079"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUN9_001079"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0694541.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUN9_001080"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUN9_001080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0694542.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUN9_001081"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUN9_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0694543.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUN9_001082"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUN9_001082"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0694544.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUN9_001083"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUN9_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0694545.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUN9_001084"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUN9_001084"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0694546.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUN9_001085"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUN9_001085"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0694547.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUN9_001086"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUN9_001086"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0694548.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHUN9_001087"
     CDS             complement(18516..18992)
                     /locus_tag="ACHUN9_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR0694549.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUN9_001088"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUN9_001088"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0694550.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUN9_001089"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUN9_001089"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0694551.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUN9_001090"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUN9_001090"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0694552.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUN9_001091"
     CDS             21688..22278
                     /locus_tag="ACHUN9_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0694553.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUN9_001092"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUN9_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0694554.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUN9_001093"
     CDS             22750..23445
                     /locus_tag="ACHUN9_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0694555.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUN9_001094"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUN9_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0694556.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUN9_001095"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUN9_001095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0694557.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUN9_001096"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUN9_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0694558.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUN9_001097"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUN9_001097"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0694559.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUN9_001098"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUN9_001098"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0694560.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7868 c         8307 g         6922 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAZOYS010000027        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOYS010000027 DAZOYS010000000
VERSION     DAZOYS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.027"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUN9_001099"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUN9_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0694561.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUN9_001100"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUN9_001100"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0694562.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUN9_001101"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUN9_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0694563.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUN9_001102"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUN9_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0694564.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUN9_001103"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUN9_001103"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0694565.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUN9_001104"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUN9_001104"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0694566.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUN9_001105"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUN9_001105"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0694567.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUN9_001106"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUN9_001106"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0694568.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUN9_001107"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUN9_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0694569.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUN9_001108"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUN9_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0694570.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUN9_001109"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUN9_001109"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0694571.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUN9_001110"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUN9_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0694572.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUN9_001111"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUN9_001111"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0694573.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUN9_001112"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUN9_001112"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0694574.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUN9_001113"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUN9_001113"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0694575.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUN9_001114"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUN9_001114"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0694576.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUN9_001115"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUN9_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0694577.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUN9_001116"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUN9_001116"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0694578.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUN9_001117"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUN9_001117"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0694579.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUN9_001118"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUN9_001118"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0694580.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUN9_001119"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUN9_001119"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0694581.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUN9_001120"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUN9_001120"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0694582.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUN9_001121"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUN9_001121"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0694583.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUN9_001122"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUN9_001122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0694584.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUN9_001123"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUN9_001123"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0694585.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZOYS010000028        29429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOYS010000028 DAZOYS010000000
VERSION     DAZOYS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.028"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..644)
                     /locus_tag="ACHUN9_001124"
     CDS             complement(<1..644)
                     /locus_tag="ACHUN9_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0694586.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="ACHUN9_001125"
     CDS             complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="ACHUN9_001125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0694587.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="ACHUN9_001126"
     CDS             complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="ACHUN9_001126"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0694588.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="ACHUN9_001127"
     CDS             complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="ACHUN9_001127"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0694589.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="ACHUN9_001128"
     CDS             complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="ACHUN9_001128"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0694590.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="ACHUN9_001129"
     CDS             complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="ACHUN9_001129"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0694591.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7181..8416
                     /gene="moeA"
                     /locus_tag="ACHUN9_001130"
     CDS             7181..8416
                     /gene="moeA"
                     /locus_tag="ACHUN9_001130"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0694592.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8416..9165
                     /gene="moeB"
                     /locus_tag="ACHUN9_001131"
     CDS             8416..9165
                     /gene="moeB"
                     /locus_tag="ACHUN9_001131"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0694593.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="ACHUN9_001132"
     CDS             complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="ACHUN9_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0694594.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10137..11036
                     /locus_tag="ACHUN9_001133"
     CDS             10137..11036
                     /locus_tag="ACHUN9_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0694595.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11042..13474
                     /locus_tag="ACHUN9_001134"
     CDS             11042..13474
                     /locus_tag="ACHUN9_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0694596.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHNCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13620..14435
                     /gene="ybiV"
                     /locus_tag="ACHUN9_001135"
     CDS             13620..14435
                     /gene="ybiV"
                     /locus_tag="ACHUN9_001135"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0694597.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14587..15852
                     /locus_tag="ACHUN9_001136"
     CDS             14587..15852
                     /locus_tag="ACHUN9_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0694598.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15819..16979)
                     /locus_tag="ACHUN9_001137"
     CDS             complement(15819..16979)
                     /locus_tag="ACHUN9_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0694599.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQCAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17217..18518
                     /locus_tag="ACHUN9_001138"
     CDS             17217..18518
                     /locus_tag="ACHUN9_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694600.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18533..20896
                     /locus_tag="ACHUN9_001139"
     CDS             18533..20896
                     /locus_tag="ACHUN9_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0694601.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21076..22671)
                     /locus_tag="ACHUN9_001140"
     CDS             complement(21076..22671)
                     /locus_tag="ACHUN9_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0694602.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22890..23810
                     /gene="ldtB"
                     /locus_tag="ACHUN9_001141"
     CDS             22890..23810
                     /gene="ldtB"
                     /locus_tag="ACHUN9_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0694603.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23869..24987)
                     /locus_tag="ACHUN9_001142"
     CDS             complement(23869..24987)
                     /locus_tag="ACHUN9_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0694604.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="ACHUN9_001143"
     CDS             complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="ACHUN9_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0694605.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25637..25765
                     /gene="mntS"
                     /locus_tag="ACHUN9_001144"
     CDS             25637..25765
                     /gene="mntS"
                     /locus_tag="ACHUN9_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0694606.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26037..27620
                     /gene="opgE"
                     /locus_tag="ACHUN9_001145"
     CDS             26037..27620
                     /gene="opgE"
                     /locus_tag="ACHUN9_001145"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0694607.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="ACHUN9_001146"
     CDS             complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="ACHUN9_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0694608.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28237..28302)
                     /locus_tag="ACHUN9_001147"
     CDS             complement(28237..28302)
                     /locus_tag="ACHUN9_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694609.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28537..29424
                     /gene="rhtA"
                     /locus_tag="ACHUN9_001148"
     CDS             28537..29424
                     /gene="rhtA"
                     /locus_tag="ACHUN9_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0694610.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7134 a         8063 c         7381 g         6851 t
ORIGIN      
        1 aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga
       61 cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac
      121 cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc
      181 ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag
      241 agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc
      301 attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc
      361 tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata
      421 agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatg
      481 atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc
      541 tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc
      601 tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca
      661 aaatcagcag aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc
      721 tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa
      781 ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca
      841 gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct
      901 ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga gataatcgag
      961 gtgccaatgc gcatggtgaa aaacaccacg atagacgaga cggtcccagg caagatatga
     1021 cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc
     1081 tgtttcaaca ccagcgtgtt gccgcgcacc agtcgggcaa acgcggggat ggaaaaaatg
     1141 gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc
     1201 agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac
     1261 cagccttcat aatatccagc caacaagccc agcaacgtcc cgatcgccgc accgataaac
     1321 acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg
     1381 tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg
     1441 tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc
     1501 acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt
     1561 cgccagaatt catgccacgg tgtacgcacc tggtcaggtt tgaccagtgg catggcgttt
     1621 aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt
     1681 taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa
     1741 gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacggagtca acgagtaagc
     1801 gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg agcaaaaagc
     1861 caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttacgtagc ccgtgtttga
     1921 ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta
     1981 aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac ggctgccact gccgcgccga
     2041 gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta ggcagccagc
     2101 ccagttcaac ggagaatacc tgaattaaaa gcatccccag cgcaaatgcc ggaaacgaga
     2161 tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg
     2221 cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc
     2281 acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg
     2341 acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg
     2401 gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgagcc
     2461 cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac aaataaaaac accagcaccg
     2521 agacgataaa cagcgtcgga atcaacccca gtaagcgttt gataacgtaa ttaagcattc
     2581 cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg tgtctggcat
     2641 gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg
     2701 gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc gggtcttttc
     2761 cgccggatca ttagttttca gtgcctgagc caggaagtcg tccacctgtt tattgctgta
     2821 aaatgcggta ttaaacagcg tcggtggcca gttttgcgag gcgaacagtg gcgatagcgc
     2881 ccagtccgct tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc
     2941 tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg
     3001 ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt
     3061 gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt
     3121 cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc
     3181 gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg caactttcac
     3241 cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg
     3301 cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc
     3361 gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg
     3421 cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt
     3481 tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac
     3541 aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc
     3601 aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc
     3661 aagaatatta ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc
     3721 gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg
     3781 atcgctggcc cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg
     3841 gaatttaatg ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata
     3901 actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta
     3961 aaacgatttc gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt
     4021 gaaattcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa
     4081 cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt
     4141 ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat
     4201 gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat
     4261 tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga
     4321 ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc
     4381 gccgtggacc aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc
     4441 gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga
     4501 gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt
     4561 cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc
     4621 gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc
     4681 gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac
     4741 gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt
     4801 cctgaaaaat aaactgaata tcccggcgca atgcctgaag tttgccgggt gacaaggtat
     4861 cgattctctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg
     4921 cccgcccggt agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag
     4981 gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca
     5041 aaccgctgcg caaagggaaa cgggtgacaa gattacgcac tcgtaaaaca ggttcgccat
     5101 ccaccaccgt tttctgctcg atgggggggg cctgtttcgc tggatgttca agcgatatca
     5161 acgggaaacg tcggggataa tccaaccctt tcatcgcacc aagttgcgga acagcagcta
     5221 acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt
     5281 ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca
     5341 tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt
     5401 gcaggatctg cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca
     5461 ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga
     5521 gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg aatgcgtacc tgatccagca
     5581 tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt
     5641 cggcaatctg ttcacccaca gtaaataccg ggttcagcga tgtcatcggc tcctgaaata
     5701 tcatcgccat atccgcaccg cgaacatggc gcatttgtgc agcgctctgc tcgctaagtt
     5761 caatcacttc gcgactgcgc cgctgcaaca gcattttatc gcactgtact aaaccgcccg
     5821 cctgttccaa caggcgcatc aatgccaacg cagtcactga cttaccggag ccggattcgc
     5881 caacaattgc cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta
     5941 ttttctgctg gtcctgcata aaggcaatat tcagattttc aaccgccagc acattaccgg
     6001 catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt
     6061 ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg
     6121 acattccctt catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt
     6181 tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg
     6241 gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ccgtgccgat acaagaaacc
     6301 gccacactgg cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt
     6361 ccgggtaatt tattggtcat tccgcctgtg gacgtggctg ccgccaaatt gccgtctaaa
     6421 tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta
     6481 tggtcgagga cggttgcccc ttccttgcgc gctgccagta gttgttcata acgcaaaggc
     6541 gtggagaaaa tctccggcga gacgcgctcc atgccacgag caaacgcaaa attttctgcc
     6601 ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc
     6661 ggattacgca gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc
     6721 acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta
     6781 aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg
     6841 cccgcttcca gcattttctg cccggtttca acaatggcag acaacgcctc aatgtagcgt
     6901 aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca
     6961 atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat
     7021 atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg atatggcatg
     7081 taaaggcagg agccaacgct acgcccgagg tggcatacag tctgtggcgt ttttctgaca
     7141 taataggcaa attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt
     7201 gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gtcaccccac tgaccgccca
     7261 ggaaacgctg ccactggtac agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc
     7321 gcttgatgtt ccggggtttg ataactccgc aatggacggc tacgcggtgc gtttagccga
     7381 tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccgtatca
     7441 tggtgaatgg cctgcgggca cctgcattcg tattatgacc ggtgcgccgg tgccggaagg
     7501 ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac
     7561 tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc
     7621 ggttgttttc ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact
     7681 ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga
     7741 tgaactccag ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct
     7801 cgccgtacac ctgatgttag aacagctggg ttgcgaggta atcaacttag ggattatccg
     7861 cgacgatccc catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt
     7921 gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga
     7981 gctgggggag atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg
     8041 taaactcagc aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac
     8101 cttctatcaa ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct
     8161 gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga
     8221 tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg
     8281 acatcagggt tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga
     8341 acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt
     8401 gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa
     8461 atcattctgc gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg
     8521 atcgtcggcc tcggtggact cggctgcgca gcttcgctgt atctggcaag cgccggtgtc
     8581 ggcaacctga cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca
     8641 ctgcacagtg atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg
     8701 cggatcaacc cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt
     8761 gcagcattga ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt
     8821 aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt
     8881 cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc
     8941 cttagccgtt tgtttggcga gaacgcatta acctgcgtag aagcaggcgt gatggcaccg
     9001 ttgatcggcg taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat
     9061 ggcaaaccga ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa
     9121 atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc
     9181 ctgataagac gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga
     9241 acgccttatc tggcctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac
     9301 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
     9361 caaacgctcc ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc
     9421 taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc
     9481 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
     9541 gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat
     9601 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcaaccctt
     9661 gtgctgcgcc atataccgcg gttcccagcg ttggaatccc ttccgctttt aacatcttaa
     9721 tagctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
     9781 gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca
     9841 gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt
     9901 tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca
     9961 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    10021 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
    10081 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga
    10141 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    10201 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
    10261 aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg
    10321 cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    10381 cggagcatct gacggcgtta accgactgct gtccgacaca ggcattaacc gtgtgtggtg
    10441 aagtgaaaag cgttgaggag atcatgacga ccgttctgcg cgataaaccg ttttacgatc
    10501 gcagcggcgg cggtttaacg ctttcgggtg gtgagccctt tatgcagccg gaaatggcga
    10561 tggcgctact gcaagccagc cacgaggcag gcattcatac tgcggtagaa acctgtctgc
    10621 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    10681 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    10741 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    10801 ttcagggctt taatgccgac gaaacctctg taaaagctat taccgatttt gccgccgacg
    10861 agctgcacgt tggcgaaatt catttcctgc cctaccacac gctaggcatc aacaaatatc
    10921 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    10981 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    11041 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    11101 ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta
    11161 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct
    11221 ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc aggcaagcga
    11281 agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga
    11341 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct
    11401 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    11461 taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac
    11521 ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg
    11581 tctgcgcgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtac tggaagattt
    11641 gcacggcgaa cagttcctga aagcgattga tatcgtgctg gtggcagtca gcgaacacat
    11701 tgaacgtttt gcagccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    11761 cgatgaactg ctggcgatag cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    11821 tttctggcag gcgctgcaac tgtgttactt catccaattg atattgcaga tcgaatctaa
    11881 cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga
    11941 cgttgaactc aaccagacac tggatcgcga acacgccatc gaattgctgc ataactgctg
    12001 gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg
    12061 aagtcctctg tatcagaacg tcaccattgg cggacaaact ctggttgatg gtcaaccaat
    12121 ggacgcggtg aatccactct cttacgctat cctcgaatcc tgcggtcgcc tgcgttccac
    12181 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc tcgacgcctg
    12241 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    12301 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg
    12361 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    12421 caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa
    12481 agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt
    12541 aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata
    12601 tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga
    12661 tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    12721 tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    12781 atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    12841 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa
    12901 cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    12961 gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    13021 tacctcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa caccggacgg
    13081 gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcctccggta ctgaccatct
    13141 tggccctact gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg
    13201 cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa
    13261 actgatgatc ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa
    13321 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct
    13381 ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgcacagga
    13441 cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc
    13501 cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg
    13561 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    13621 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    13681 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg
    13741 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    13801 agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc
    13861 acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc
    13921 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag
    13981 catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa gattatcagg
    14041 agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa atcccgttag
    14101 tgatcgacaa actgcacata gcgctcaatg gcattatgaa acccgtcacc agtggttttg
    14161 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    14221 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    14281 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    14341 aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg
    14401 cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc cgtgcgctcc
    14461 ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt
    14521 atttataact tatattgtcg catgcaaatc agaatggttt cgaatgaaag ttaatgagag
    14581 gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc
    14641 cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag
    14701 ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat
    14761 gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag
    14821 cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa
    14881 gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc
    14941 tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac
    15001 cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg
    15061 cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg
    15121 gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa
    15181 ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag
    15241 cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca
    15301 catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg
    15361 acattccaag gctggacagc gctctctaat atgctgcctg gtcaggggct gctgcacgtt
    15421 gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg
    15481 gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat
    15541 ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg
    15601 tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac
    15661 gtgatgtaca ttcctgcggc accaatgtgc gagaaaaatc ttgcctacgc gcacaaggtg
    15721 aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca
    15781 aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttggga
    15841 atcgacagct gagtgtcgta cagcccttcc cgttccacgg cggtgttccc ttttcctggg
    15901 cgaacccgcc gcactgactc ccgcaccgcc aatcgtccgt gcagcagcag cgagcccacc
    15961 ggagcatccg ggcgaatcag ccgctgttgc aacatttgca ccgcttcatt gcctagctca
    16021 tcgcgtggca catgcacagc cgtcagggga acatcctcga ttgccgcaag gttaaagccg
    16081 tcgatactca tcaccgagac atcctgtggt acgcgcagac cacgacgatg tagcgcattt
    16141 accgttcccg ccgccataaa atccccgccg accagaaacg ccgtcggcaa cgtcttgcca
    16201 ttggcggcat ccagccacgc tccgaccttt tcttccgctt ctttagcgct aaaactgctg
    16261 atattcagca aatgacggtc atggtgaaag gccagattat gctgacgcca tgattcttta
    16321 atccctgcaa accgctgctc catagtgtag cggcgcaggc acatcacatt catcacctcc
    16381 cggtgaccca tttcaaacag atagcgggcg gcgaacgcgc caattaaccg atgatccggc
    16441 gcaatcgccg ggaggcgcat tcgttcatca cggcagttga tcagcacaca aggcttagcg
    16501 agatcggcgg ccagatcatg gatgtgcgga tcgtcaatcc ccagcaggat cgctgcctgc
    16561 gtatctgctt cattcatccg cgagagaaag agcgcgggat cgctgtcaaa ctcctccagc
    16621 gcgcagtaac gcagccgcac ttcatgcggc gataacgctt tgctgatgct ctggatcaca
    16681 cggtaataaa agatatcaga tcgttggtca aaagcgcatt gcggcgcgaa aataatcagg
    16741 ttgttcagca acatacgacc tgctgccatg ccatccatca cgcccaactc acgcgcgcag
    16801 gccagcacct gttcgcgagc gcgtgcactg gtattcgctt tgccagccag cacccgcgaa
    16861 accgtactcg gcgatagccc ggtacgtgcg gcgatatcgg cgattttcag cttcttgtcc
    16921 attttgtgat ctgcctctaa ttcggaaatg acagaatttt catgagcaaa aaagcccatt
    16981 ctgtctggca ttaaatccat ttcagtaatg tatatcagtc actcatggaa aaacgtgcac
    17041 aaaacccgct ttctttcgct tttagggtct cttactgtga attggtagaa caacttccat
    17101 acaaaaataa tggaaaaacg aacaataaag actttattta tcaaaaagtt aaaagtggtg
    17161 taacaactcc attctgacgt tgccgcacaa accctatacc attctgtgga gtgcatatga
    17221 gtcagggtat caataacgat atgactgcca gtaaaccgcg ccatatcgtc aaaaatctgc
    17281 gttggtggat actggtcctt tttttgctcg gtgttactgt caactacatc acgcgtaact
    17341 cgctgggtat tctcgccccc gaactgaaaa gcagcctagg gattaccacc gaacaatatt
    17401 cctggattgt aggtgctttc cagctcgcct ataccctatt ccaaccgttg tgcggatggc
    17461 tgattgacgt cattggcctc aaactgggct ttatgatctg cgcgggactg tgggcactgg
    17521 cctgtctgat gcatgcgggg gcgggaaact gggtacatct ggcgctgttg cgcttcttta
    17581 tgggcggtgc ggaagccgcg gcaaccccag caaacgccaa aaccatcggt gaatggtttc
    17641 caaaatcaga acgcccgatc gccgcaggct gggcaggcgt cggtttttcc atcggtgcca
    17701 tgctggcccc accgattatc tattttgccc acgcttcatt cggctggcag ggtgcattta
    17761 tgttcactgg catcctggcg atgctgtggg tggtgctgtg gtgggcgttc tatcacagcc
    17821 cggataagca tcctaacctc agcaaagccg agctggactt tattcgccag gataacgaag
    17881 cgcccccggt caagttaccc tttctgaccg cactgaaaac cgtgggcaaa aataaacgct
    17941 tttacggtat cgctatcccg gcctttatgg cagaaccggc gtgggcggtg atgagtttct
    18001 gggtgcctct atacctgtcc aaaacctatg gcatggacct gaagcagatc gcattattcg
    18061 cctggttgcc gttcctcgcc gctgatcttg gcagtattgc cagcggctat ctgaccaaac
    18121 tttacacccg cctgtttggg ctgagtcgtg tgaactccgt tgtcgcatca tcggtcagtg
    18181 gcgcattctt gatggtttcg ctggcggtga tggcctttac caataacccg tatatcgcca
    18241 tcgtccttat ctccatcggc ggcttcggtc accagatcat ctcctgcatg ctgagcgcac
    18301 tggtggtgga atccttcgac aaaggtcaga tggccactgt taacggcatg cgcggttctg
    18361 ccgcgtggat cgcaagtttc ctgttctccc tgattatcgg ggttaccgct gacaaaatcg
    18421 gctttaaccc cctgtttatc gccatgggct tctttgacct gattggcgct ttcttcctgg
    18481 tcacctttat tgctgaacgt cgcgcaaaac gcgcctgaga gtggatgtaa acatgaaaac
    18541 gctgaaaaac tggaccctgc acaaacagtc tgcccaccac gttgaactgc tggtcgatgg
    18601 ccagcatatc ctgtgcctgt acgtgctgga agaaaatctg ttccgcgtgc tgttaaaacg
    18661 caaaggcgag ctggcgctag atcgcacctg gagcattgct ccggcgcagg atgtgccatg
    18721 ggaagggcgc agccgcgaag atctcagcgg ctttagcctg cccgcgtgga cagtgatcca
    18781 gcacgacgac accgtggtta tcgccaccga gtcactgcgt gcaaccgttc accagccgct
    18841 gtggctggag tggcactatc gtaacaacga aggcgaatgg ctgccgttgg tgaatgaccg
    18901 cccgaccagc gcttatctgc tgaatgccca cggcgatggc gtggcacact atcagagccg
    18961 tcgtaaagat gaacgctttt atggcctcgg agataaagcc ggcgatctgc aacgcaacgg
    19021 taaacgttac gagatgcgta acctcgacgc gatgggctac aacgccgtta gtactgaccc
    19081 gctgtacaaa cacattccat tcaccatcac ccgccgcgat gacattagct tcggcctgtt
    19141 ttatgacaac ctcagcagct gctggctgga tctgggcaac gagatcgaca actaccacac
    19201 cgcctatcgc cgctggcagg cggaagcggg tgatatcgac tactacctgt ttaccggcga
    19261 acgcgtactt gatatcacca aagccttcgt acgcctgacc ggaaaaaccc tgttcggacc
    19321 gaaatggagc ctgggctaca gcggttcaac tatgcactac accgatgcgc cggacgcaca
    19381 aaatcagttg atgaacttta tccgcctgtg tgaagaacat gcaattccct gcgattcgtt
    19441 ccagctctct tccggttata cctcaattaa cggcaaacgc tatgtgttca actggaacta
    19501 cgacaaagtg ccgcagccga aagtgatgag ccaggcattt cacaatgctg gtttgcacct
    19561 ggccgccaat atcaagccgt gcctgttgca ggatcaccca cgctataacg aagtggcaga
    19621 aagaggactg ttcatccgtg attcagaaac agatgaacca gaacgttcca gcttctggga
    19681 tgacgaaggt tctaacctcg actttactaa tccgcagacc atccagtggt ggcaggaagg
    19741 cgtgaccaca cagcttctgg agatgggtat cgactccacc tggaacgaca acaacgaatt
    19801 cgaagtatgg gacggtgaag cacgctgtca cggctttggt cagacgatcg cgatcaaaca
    19861 tattcgccca gtgatgccac tgctgatgat gcgagcctcg atggaagccc agcagcgttt
    19921 cgctccggaa aaacgtccgt acttgatctc ccgctccggc tgcgcgggaa tgcagcgcta
    19981 cgtgcagacc tggagtggcg ataaccgcac tagctgggac actctgcgct acaacacccg
    20041 catgggtctg gggatgagcc tctccgggct gtataacctc ggtcacgacg tcggcggttt
    20101 ctccggtgat aagccggacc cggaactgtt tgtacgctgg gtacaaaacg gcgtgatgca
    20161 tccgcgcttt acgattcact catggaacga tgaccatacc gtaaacgaac cgtggatgta
    20221 tccgggcgtc actccggcga tccgcagcgc tattgagttg cgctatcgcc tgctgcccta
    20281 cttttatacc ctgatgtggc aggcatacgc cgatgatgaa ccaatgctac gtccgacttt
    20341 ccttgaccac gagcacgacg ttcagacttt tgaagagtgt gatgacttcc tgcttggtcg
    20401 cgatattttg gttgccagcg tcgtagagca aggcgagcgc cagcgccgtg tatggctgcc
    20461 agataacgaa actggctggt acgatttcta caacggcgaa tggttcagtg gcggtcagtg
    20521 gatcaccctc gacgcaccgc tggagaaact gccgctgatg gttcgtgccg gggctggtct
    20581 accgctcagc aaacgcatca cctatgtgag cgcagaacaa gacgataccc gcgaactgaa
    20641 actgttcccg ctgaaaggtg tgggcacaac gtccggcctg ctgtttgaag acgatggcga
    20701 aagctggggc taccaaactg gtaatgcgct ttgggtggaa tgggaaatgg tttgcgatgg
    20761 ggcgaccatc aacctgaaag tcaacgcgcg cggggattat cgtcctgcat ggagtgcact
    20821 gaaagtgtca ttaccattgg aagaaaaacg cacgctgctg gtcaatggtg ttgaagggag
    20881 tgaatggatg cgttaaatca ttaccggggc cgggaggcga ctgcacctta cccggtccag
    20941 ccatcgaatc ctttacctca gtatttcacg ctgcaggaag gcagcaaacc tgtaaacccc
    21001 gggagcttaa ataagtaagt gaccggggtg aacaggtgaa gccaatgcat ctgccgcatg
    21061 aaagacggcg gggaattaag cgtcgatccc tttactacgc aggtaatctt cgtaattacc
    21121 gctaaagtcg atcgcgcgtt ccggggtgat ttccagaatg cgggtcgcca gggagcttac
    21181 gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga tacagttcca gcgccatgtt
    21241 cagtgactcg atggattcca tatccaggtg gttggtcggt tcgtccatga tcagaatgtt
    21301 cggcttctgc atcattaact taccaaacag catccgccct ttttccccac cggaaagcac
    21361 tttagctggc tttttgatgt cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac
    21421 cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc cattcgaaca cggtcagatc
    21481 attttcaaac tcatattcgt ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca
    21541 ttttacggtg ccgctgtccg gttgcagatc gcccaccagc gttttcagca gcgttgattt
    21601 accgacgccg ttggtaccca gtaccgcaag tttttcaccg acctccagca gcaggttgag
    21661 atttttaaac agtggaccgt tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt
    21721 acggaacagt ttcttatcct gttcaaaacg gatgaacggg ttctgacggc tggaggcttt
    21781 cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt
    21841 cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac tcagcaatct gcgctttctt
    21901 cttggcgtta tcggccagca gacgttcacg cgcctgggtc gccgccgtca tgtactcatc
    21961 gtagttaccc ggataaacgc gcagctcgcc gtaatccaga tccgccatgt gggtacagac
    22021 catgttaagg aagtgacggt cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag
    22081 cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag
    22141 gagaatatcc ggatcagcaa acagcgcctg cgccagaagc acacgcagtt tccagccagg
    22201 agcaacttca ctcatcgggc cgtagtgctg ttccactgga attcccacgc caagcaacag
    22261 ttcaccggcg cgagcttccg cagagtaacc gtccatttcg ccgtatttaa cttccagatc
    22321 ggccacttta tagccgtctt cctcactcat ttccggcaaa gcatagatgc ggtcacgctc
    22381 ctgcttcact tcccacaact ctttatgccc catgatcacc gtatccagca cagtgaactc
    22441 ttcaaaggca aactgatcct gacgcagctt accaatgcgc tcgttgggat cgagggaaac
    22501 gttacccaaa gtcggctcta agtcgccgcc gaggatcttc ataaaggtgg atttaccact
    22561 accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg aatttgacgg aaatgttttc
    22621 aaacaacggc ttactgccga actgcatggt gacgttactg gaaactaaca caggcgtatc
    22681 ctgaaaagag atatgacaaa ccgcgcatta tgccataagt ctgaaagaag atcctgctcc
    22741 tcgcttaagg tctacactca agccaaaggc aaaaaatgtg atttcgtaca catctgattt
    22801 cactgtgagc tggaatgaac ttataatgcg cttccaatac tctaatattc tcaacccaat
    22861 ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag
    22921 cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc ggtaacttat cctctgccaa
    22981 ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac cattcctgaa ggtaacactc
    23041 agccgctgga gtattttgcc gcggagtacc agatggggct ttccaatatg atggaagcga
    23101 acccgggtgt ggataccttc ctgccgaaag gcggtactgt actgaacatt ccgcagcagc
    23161 tgatcctgcc ggataccgtt catgaaggca tcgtcattaa cagtgcagag atgcgtctgt
    23221 attactatcc gaaagggacc aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag
    23281 gcaaagatac gcctatcaac tggaccacca aagttgagcg taagaaagca ggcccgacct
    23341 ggacgccgac cgccaaaatg cacgcagagt accgcgctgc gggcgaaccg cttccggctg
    23401 tcgttccggc aggtccggat aacccgatgg ggctgtatgc actctacatc ggtcgcctgt
    23461 acgctatcca tggcaccaac gccaacttcg gtatcggcct gcgtgtaagt catggttgtg
    23521 tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg
    23581 tacagtttat tgatgagccg gtaaaagcga ccaccgagcc agacggcagc cgttatattg
    23641 aagtccataa cccgctgtct accaccgaag cccagtttga aggtcaggaa attgtgccaa
    23701 ttaccctgac gaagagcgtg caaacagtga ccggtcagcc agatgttgac caggttgttc
    23761 ttgatgaagc gattaaaaac cgctccggga tgccggttcg tctgaattaa tcttcaaaac
    23821 ttaaagcaaa aggcggactc ataatccgcc ttttttattt gccagaccct agttggccgg
    23881 gagtataact aacaaaacat atcccaccaa cgccgcccac aacagcatcg gtattgaata
    23941 gagatggaaa cgccaccaga tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc
    24001 cagcgatccg ggtaataacc caaagccacc tacgtttacc gcccatacca gtaataaaga
    24061 cggcggcaca tagttcagca acaatatggt actcggcacg ttactgatca cctgcgataa
    24121 accgattgcc gttaaccata acccgggttc agatagatga ctcacgttac ccaacacgcc
    24181 ttgcaacgct ggaagctggg tcagtaaatg gacgtcgata aacatcgcca taaacaccag
    24241 cagcagcgtc cagtccacac tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc
    24301 cacaatcacc agtccccaca gctcttgttt gaactccagc gccgtcagaa agacgatata
    24361 cagccccaga caactccaca ccagccgcgg tttccactcc ggtgtttgca cccccgtatg
    24421 gtattgcagc gcctttccag ggaaacaaca ccagcacagg agcaggagcg tcagcatcat
    24481 tgcgccagcc agcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc
    24541 ccagataaga atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc
    24601 cagcgcctca aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat
    24661 agtcagcgga acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc
    24721 cgccgccagc accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt
    24781 gcgccccagc acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc
    24841 gcttaaggtg atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaggg
    24901 cacgaaaaag cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg
    24961 cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa
    25021 gcgtcgagcg tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt
    25081 tccggactga cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt
    25141 tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca
    25201 atcagcccca tggttgccag ccgcttaagc attttagcca ccgtcggttg cgaaactccc
    25261 agacgagcag ccatgtccac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc
    25321 agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct
    25381 tcaacgtgct cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg
    25441 cgacgactca ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg
    25501 cacattgtaa accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctacata
    25561 atatagcctg tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag
    25621 caagtcagga ggtcttatga atgagttcaa gaagtgtatg cgcgtgttta gtcattctcc
    25681 ctttaaagta cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca
    25741 gcaagataaa ccttccgata aatagcggcg tcgcggtacg ccgcttcact cctgctttta
    25801 tgcaggcata acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct
    25861 tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg gctcgctcta tcttcttgca
    25921 gccctgcgta tattgcggct cgcggatgcg gacccctttc ccctcttcac gcactcttgc
    25981 aggtattgac ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga
    26041 atttaaccct caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt
    26101 tctacttttt acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct
    26161 acaccgctgc gtttacggct attttgcttt tgctatggcg aacattgcct cgcgtacaaa
    26221 aagttctggt cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct
    26281 acggcgcgcc taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga
    26341 ccgaaatcct gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc
    26401 tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg
    26461 acagcctgtg tctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg
    26521 cctggggtgg cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg
    26581 atgtcatcgt caataataac gaggtgattg aagagcaaga acggatggca aaactttccg
    26641 gaatgaaaga tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg
    26701 tgatcggcga aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata
    26761 ccccgtttgc cagcagcgtt aacggtttga tatttgctga ctacattgcc gccagtggct
    26821 ccacgcagaa atcacttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc
    26881 aggataactt tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc
    26941 agggccaaat cggcgaatac gatactgcta tcgccagcat cgccaaacgt gcagatgaag
    27001 tgtacttcct gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac
    27061 tggatatgac cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctgc
    27121 atctgatggg ctcgcatccg caggcctgcg acaggacaca aggcaaatac gaaacctttg
    27181 tgcaatcgaa agaaacgtcg tgctatctct ataccatgac gcaaacggac gatttactgc
    27241 gcaagctgta cgatcagtta cgcaacagcg gcagcagctt ctctctggtt tacttttctg
    27301 accacggtct ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata
    27361 aatatcagca aaatttccag gtgcctttta tggtcatttc cagcgacgat aaagcgcatc
    27421 gggtgattaa agcccgccgc tcagccaatg acttcttagg ctttttctcg cagtggacgg
    27481 gaattaaagc gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc
    27541 cgatatacat caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata
    27601 ttttcgaccc gaaaccttaa aacaaaaaat ccgccccgag aggcggattt tttatatcac
    27661 caaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    27721 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    27781 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    27841 gtctggaatt taccataacc cacccccact acaccgtaga tgcttgccca gtcgttaatg
    27901 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    27961 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    28021 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    28081 ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa
    28141 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    28201 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    28261 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    28321 atttaaatca ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat
    28381 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    28441 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    28501 cgctttactt aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    28561 ccggtctggt taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc
    28621 tcgttagcta agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg
    28681 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    28741 gagcaacggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    28801 tatctttcta ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca
    28861 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    28921 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    28981 ggctgtgcgc tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    29041 cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta
    29101 attttcgtgc caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt
    29161 ccattgggtc tggctgtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt
    29221 gccctcaccc gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg
    29281 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    29341 ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa
    29401 ataaaagaat tagacattaa ttaaattta
//
LOCUS       DAZOYS010000029        29308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOYS010000029 DAZOYS010000000
VERSION     DAZOYS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.029"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..877)
                     /gene="nadA"
                     /locus_tag="ACHUN9_001149"
     CDS             complement(<1..877)
                     /gene="nadA"
                     /locus_tag="ACHUN9_001149"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0694611.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
     gene            complement(1126..1257)
                     /locus_tag="ACHUN9_001150"
     ncRNA           complement(1126..1257)
                     /ncRNA_class="other"
                     /locus_tag="ACHUN9_001150"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1282..1357)
                     /locus_tag="ACHUN9_001151"
     tRNA            complement(1282..1357)
                     /locus_tag="ACHUN9_001151"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Lys,seq:ttt)
     gene            complement(1391..1466)
                     /locus_tag="ACHUN9_001152"
     tRNA            complement(1391..1466)
                     /locus_tag="ACHUN9_001152"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Lys,seq:ttt)
     gene            complement(1613..1688)
                     /locus_tag="ACHUN9_001153"
     tRNA            complement(1613..1688)
                     /locus_tag="ACHUN9_001153"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Lys,seq:ttt)
     gene            complement(1692..1767)
                     /locus_tag="ACHUN9_001154"
     tRNA            complement(1692..1767)
                     /locus_tag="ACHUN9_001154"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1732..1734),aa:Val,seq:tac)
     gene            complement(1917..1992)
                     /locus_tag="ACHUN9_001155"
     tRNA            complement(1917..1992)
                     /locus_tag="ACHUN9_001155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1957..1959),aa:Lys,seq:ttt)
     gene            complement(2157..2948)
                     /gene="cpoB"
                     /locus_tag="ACHUN9_001156"
     CDS             complement(2157..2948)
                     /gene="cpoB"
                     /locus_tag="ACHUN9_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0694612.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(2958..3479)
                     /gene="pal"
                     /locus_tag="ACHUN9_001157"
     CDS             complement(2958..3479)
                     /gene="pal"
                     /locus_tag="ACHUN9_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0694613.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(3514..4806)
                     /gene="tolB"
                     /locus_tag="ACHUN9_001158"
     CDS             complement(3514..4806)
                     /gene="tolB"
                     /locus_tag="ACHUN9_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0694614.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(4939..6171)
                     /gene="tolA"
                     /locus_tag="ACHUN9_001159"
     CDS             complement(4939..6171)
                     /gene="tolA"
                     /locus_tag="ACHUN9_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0694615.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(6236..6664)
                     /gene="tolR"
                     /locus_tag="ACHUN9_001160"
     CDS             complement(6236..6664)
                     /gene="tolR"
                     /locus_tag="ACHUN9_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0694616.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(6668..7360)
                     /gene="tolQ"
                     /locus_tag="ACHUN9_001161"
     CDS             complement(6668..7360)
                     /gene="tolQ"
                     /locus_tag="ACHUN9_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0694617.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(7357..7761)
                     /gene="ybgC"
                     /locus_tag="ACHUN9_001162"
     CDS             complement(7357..7761)
                     /gene="ybgC"
                     /locus_tag="ACHUN9_001162"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0694618.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(7911..8204)
                     /gene="ybgE"
                     /locus_tag="ACHUN9_001163"
     CDS             complement(7911..8204)
                     /gene="ybgE"
                     /locus_tag="ACHUN9_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0694619.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(8204..8317)
                     /gene="cydX"
                     /locus_tag="ACHUN9_001164"
     CDS             complement(8204..8317)
                     /gene="cydX"
                     /locus_tag="ACHUN9_001164"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0694620.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(8332..9471)
                     /gene="cydB"
                     /locus_tag="ACHUN9_001165"
     CDS             complement(8332..9471)
                     /gene="cydB"
                     /locus_tag="ACHUN9_001165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0694621.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(9487..11055)
                     /gene="cydA"
                     /locus_tag="ACHUN9_001166"
     CDS             complement(9487..11055)
                     /gene="cydA"
                     /locus_tag="ACHUN9_001166"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0694622.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(11903..14536)
                     /gene="mngB"
                     /locus_tag="ACHUN9_001167"
     CDS             complement(11903..14536)
                     /gene="mngB"
                     /locus_tag="ACHUN9_001167"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0694623.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(14554..16470)
                     /gene="mngA"
                     /locus_tag="ACHUN9_001168"
     CDS             complement(14554..16470)
                     /gene="mngA"
                     /locus_tag="ACHUN9_001168"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0694624.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            16639..17361
                     /gene="mngR"
                     /locus_tag="ACHUN9_001169"
     CDS             16639..17361
                     /gene="mngR"
                     /locus_tag="ACHUN9_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0694625.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(17465..18334)
                     /gene="sucD"
                     /locus_tag="ACHUN9_001170"
     CDS             complement(17465..18334)
                     /gene="sucD"
                     /locus_tag="ACHUN9_001170"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0694626.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(18334..19500)
                     /gene="sucC"
                     /locus_tag="ACHUN9_001171"
     CDS             complement(18334..19500)
                     /gene="sucC"
                     /locus_tag="ACHUN9_001171"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0694627.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(19594..20811)
                     /gene="odhB"
                     /locus_tag="ACHUN9_001172"
     CDS             complement(19594..20811)
                     /gene="odhB"
                     /locus_tag="ACHUN9_001172"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0694628.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(20826..23627)
                     /gene="sucA"
                     /locus_tag="ACHUN9_001173"
     CDS             complement(20826..23627)
                     /gene="sucA"
                     /locus_tag="ACHUN9_001173"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0694629.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(23928..24644)
                     /gene="sdhB"
                     /locus_tag="ACHUN9_001174"
     CDS             complement(23928..24644)
                     /gene="sdhB"
                     /locus_tag="ACHUN9_001174"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0694630.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(24660..26426)
                     /gene="sdhA"
                     /locus_tag="ACHUN9_001175"
     CDS             complement(24660..26426)
                     /gene="sdhA"
                     /locus_tag="ACHUN9_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0694631.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(26426..26773)
                     /gene="sdhD"
                     /locus_tag="ACHUN9_001176"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(26426..26773)
                     /gene="sdhD"
                     /locus_tag="ACHUN9_001176"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0694632.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(26767..27171)
                     /gene="sdhC"
                     /locus_tag="ACHUN9_001177"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(26767..27171)
                     /gene="sdhC"
                     /locus_tag="ACHUN9_001177"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0694633.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            27552..27659
                     /locus_tag="ACHUN9_001178"
     CDS             27552..27659
                     /locus_tag="ACHUN9_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694634.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            27865..29148
                     /gene="gltA"
                     /locus_tag="ACHUN9_001179"
     CDS             27865..29148
                     /gene="gltA"
                     /locus_tag="ACHUN9_001179"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0694635.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         7091 a         7883 c         7158 g         7176 t
ORIGIN      
        1 tgcggcaggt tgcaccctca cctgcggttg gtgcttccag taactcttta tctggcaccg
       61 cctgctgcat tttgtagaaa atgccccgat cggttgccac gataagccgc tgatgtggca
      121 atgttttcgc agcagcgatc agttgactgg tggaaccgac cgcatccgcc atatcgacaa
      181 tagcttgtgg tgattctgga tgcaccagta tggcagcatc cgggtattct tcttgcaagc
      241 gggttaacgc ctgagtctta aattcatcat gcacaataca ggcaccctgc cagcataaaa
      301 tgtctgcgcc cgtctgtttt tgcacgtaac gccccagatg tttgtcgggt gcccagatga
      361 ttttttcacc aaaactatcc agatgatcaa taagttcgac ggcaatgctt gaagttacca
      421 cccaatctgc gcgcgctttt accgcagcag aagtgttggc gtagacgacg acagtatgat
      481 cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg gcagccgaga tccagtgaac
      541 attcagcctg aagtgtcagc atcagaattg ttttttccgg actgagaatt ttggcggttt
      601 ctcccataaa tctcacccca gcgactaaca acgtagaagc gggatgcttt gcaccgaagc
      661 gcgccatttc cagagaatca gaaatacagc caccggtttc ttctgccagt tgttgaattt
      721 cggggtcggt atagtagtgg gcaaccatca ccgcattacg ttcttttagc agacgtttta
      781 tcttctcgcg gtaatacgct ttttcatcaa tgcttaacgg cgtcggcttc ggggggaaag
      841 gataaatcgc cgtgtctgga tcaaacatca cgctcatctt accatctcgt tttacaggct
      901 taacgttaaa accgacatta gcgtaatatt cgctactatc ggcgtaattt gttttatatg
      961 ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa ttacgcctaa ttccaccgac
     1021 gtagagttgc ttgcacagta agctctgacg atgtcatcag gtttacaaag tgaaattgag
     1081 gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag
     1141 gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag cggctccgtt gtttcgctgc
     1201 gctcaactcg aacctgctgc aggttcgaat ctttgaatat ctcagatagc aaaaaagcgc
     1261 ctttagggcg cttttttaca ttggtgggtc gtgcaggatt cgaacctgcg accaattgat
     1321 taaaagtcaa ctgctctacc aactgagcta acgacccctt gcgggattat gctgctttaa
     1381 tcattcagga tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
     1441 tgctctacca actgagctaa cgacccatac gggtgttacc tgaaagattt ctactcgggg
     1501 ccaatcttta agattggtgg gtcgtgcagg atgactcgct tcgcttgccc tacgggccgt
     1561 tgctggcgca acgttatcct tcacgcttaa catctgagtt tgatgttaaa attggtgggt
     1621 cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct
     1681 aacgacccga gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag
     1741 gtgctctccc agctgagcta atcacccgat actgcgctgg atactacaaa ctacttccac
     1801 cgaacccacc gtaattagtg gtgggtcgtg caggatgact cgcttcgctc gccctacggg
     1861 ccgttgctga cgcaacgtta tcctacacgc ttaacatctg tcttcgatgc taaaactggt
     1921 gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg
     1981 agctaacgac ccactttttc gttgctttcg gtttgtttga tatcccgtgg caacggcggc
     2041 atatattact gatttcagac ttgagcgcaa caaaaatttc gatgcagatc actcaactgc
     2101 ttatgattcg cacgacacga ccagaaataa tgcgacttct ggtcgtgtgt tatgcattac
     2161 atcgcgttca gacgtttttg tgcctgttta gcgccatcag taccagggta tttactgata
     2221 acctgctggt atacggcttt cgcttttgcg gtgtcacctt tgtcctgcat gatgacgccg
     2281 actttaaaca tcgcatcagc agcctttggt gacttcggat agtttttcac taccgaagca
     2341 aaatagtacg ccgcatcatc ttttttaccc ttgttgtagt ttaactgacc cagccaataa
     2401 ttggcgtttg gcaggtaagt tgaatcaggg taatttttga tgaaattctg aaatgccacc
     2461 attgcgtcat cctggcggga tttatcctgc accagcgcaa tagctgcatt gtaatccgtg
     2521 tttgcatcgc cgcttttcac cggcgcgcca gcattcgcag taccagcatc agctgtcggc
     2581 gtcgttgatg ccgccgcacc gctttgatcg ctgctggttg attgcgccgc tgcaccaccg
     2641 ctgctgaggc tgtcgatctg caacaggatc tgcttttgcc gctccacgac ctgattcagt
     2701 tgatactgat tttcctgaat ctgaccacgc agggaatcaa tatcggattg attatcagaa
     2761 agttgttgct ggagttgggt taaaagctgg ctgtgagcat tagaaatacg ctcaagttga
     2821 gtgacgcggt cttcgaccga gcctgagccg acactactga ttggtgcctg agcaaaagcg
     2881 gcccaggggg ccgctatacc aaccagtaac gacagactca atagttgatg tctgaagtta
     2941 ctgctcatgc aattctctta gtaaaccagt accgcacgac ggtttttgga gtatgccgct
     3001 tcgtcatgac ccagtactgc aggtttttct ttaccgtaag aaacgatgga gatctggtct
     3061 gcagaaacgc ctttaccctg caggtacatc ttaacggcgt tcgcacgacg ttcacccagg
     3121 gagatgttgt attccggagt accacgttcg tccgcgtgac cttctacggt gactttgtaa
     3181 gacgggttgc tacgcaggaa gtttgcatgt gcatccagca tttgagcgaa gtcagaacgg
     3241 atatcgtact tgtccagatc gaagtaaacg atgttgttct gctgcagctg ttgcatttgc
     3301 agacgagcct gctcttcgga agacatgttg ccgttgccgc cgttcgcatc cataccagtg
     3361 ccggcaccca gcatgccttc gctgccgtca ttgctggcgt tcttgttgga agaacatgcc
     3421 gcaattgcca taacaggcag agcaatcatc agccctttca gcactttgtt cagttgcatt
     3481 tcaatgattc ctttactatt caattaatta ttatcacaga tacggcgacc aggcagggaa
     3541 tttgacctgt ccatcagttg ccggaagacg cgctttgaaa cgcccatctg tagaaaccaa
     3601 attcagcacg gatcccatcc cctgagaaga gctgtagatt accatagtgc cgttaggtgc
     3661 cagacttggc gtttcatcca ggaacgtgga cgacagaact tgtacgcctc ccgttgccag
     3721 atcttgtttg gcaatgtgct gctgcccacc gttggagctg accattacca taaatttacc
     3781 gtcgctgctg acatccgcat cctggttctg cgaaccttcc caggtaatac gttgtggcgc
     3841 accgccgttg atattcactt tataaacctg tggacgaccg gcctggtcag aagtaaatgc
     3901 caggttctgg ctgtccggga accaggtcgg ttcggtattg ttactgcgac catcagtcac
     3961 ctggcggatc tgaccagaag ccaaatccat tacgtacagg ttcaggctac cggttttcga
     4021 caaggcgaat gccagtttgc tgccgtctgg cgagaatgca ggcgcaccgt tgtgacgcgg
     4081 gaatgaagcc acctgacgta cagcgccgtt tgccagcgtt tggataacta gcgcggaacg
     4141 accgctttcg aaggtcacat aagccagttt agaaccgtct ggtgaccacg ccggggacat
     4201 cagcggctgt ggtgaacggt gaacgacaaa ctggttgtaa ccgtcatagt cagatacgcg
     4261 cagttcatac gggaactgac cgccgttggt ctgaacaacg taggcaatac gggtacggaa
     4321 cgcaccttta atgccggtca gtttttcaaa cacttcatca ctggcggtat gaccagcata
     4381 gcgcagccac tgcttgttca ctttgtacga gttctgagca agtacagtac ccggtgcgcc
     4441 gccagtgtca acaagttgat aagcaacatt gtaagaacca tccggattcg gagtgacctg
     4501 accgacaact acagcgtcaa tacccagcgc ggaccatgca gctggttgta cttcctgcgc
     4561 actacccggc tgctgtggca gacgagcgcg atctaacgga ttaaatttac cgctgttacg
     4621 caagtcggca gcaacgatgc cgccaatatc ttcaggtgcc gcaccagggc ccgcccactg
     4681 gaaaggaaca acaccaatag gacgaccgga atctacaccg ctgtcgatca caatgcggac
     4741 ttcagcatgc agaactgatg cccacagtat gagaaaacca aatgctactc gtaatgcctg
     4801 cttcatcata tctcccttat ctggaccgat ggcccacgat aatttagcag aatgttaaca
     4861 aactaaaata cacaaaacta ccagaacccc gtggcaaccg gtgcctgatg ttgaccgtcg
     4921 gaacagtcaa catcgcgatt acggtttgaa gtccaatggc gcgtttttga acacttcata
     4981 tactgcctgg cttggtggtt tcgggatctt cgcaagttta gctgctgcca acgcagcctg
     5041 acaaagtgcg ggatcgccac cttcaggttt gatatccagt aacataccat cgggtgccag
     5101 ttttatgcgc agcgtacagg ttttgcctgc ataggacgat gcgtcataga acttactttc
     5161 gatagcagat ttaatctgcc cggcatagtt attgatatcg gcccctgatg cgccattgtt
     5221 tttagtatta ccactcccgg caggcgaagc attgttccct ttcgcccctc cccccgtttt
     5281 cggtgcattc ttaccagagc ttagctcacc gaaaatatca tctgcctctg cggcagcttt
     5341 tgctgcagcg gctttttcag ctgctgcttt tgccgctgcc gcttttttgt ctgcggcggc
     5401 tttttctgcc gctgcttttg ccgctgccgc ttttttgtct gcggcggctt tttctgccgc
     5461 tgctttctta tcagctgctg ccttatcagc agccgctttt tctgctgctt ttttgtcggc
     5521 tgcagctttc tctgctgccg ctttctttct tgcttcagct gcagcagctt ctgccgcttc
     5581 cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctt cggctgcggc
     5641 tttggcggct tctgcttctg cttttttctt cgcgtctgcc gccgctttct tcgctgcttc
     5701 ttccgcagct ttagcatccg cttcggcctt cgctttagca tctgccgccg ctttcgctgc
     5761 cgcctcttcc gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
     5821 tttctgttcc tgagcagcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
     5881 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc tttatcttgc gctgctcatc
     5941 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
     6001 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
     6061 attctcatcg aacgaactcc agagcagcgc cgcaaataag atgacatgca gcactgctga
     6121 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
     6181 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
     6241 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
     6301 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
     6361 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
     6421 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
     6481 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
     6541 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
     6601 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
     6661 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
     6721 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgaggc
     6781 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgcagtc gcaatcaacg
     6841 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
     6901 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
     6961 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
     7021 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
     7081 gcaggcgcac aaactctttg aacccgctgt aaaatatttg ttccgaacca gtcagattat
     7141 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
     7201 cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat acgggtccgc tggataataa
     7261 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
     7321 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
     7381 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
     7441 tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt
     7501 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
     7561 gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
     7621 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
     7681 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
     7741 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
     7801 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
     7861 aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
     7921 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
     7981 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
     8041 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
     8101 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
     8161 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
     8221 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
     8281 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
     8341 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
     8401 ccaagcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccaag tcatgacgtt
     8461 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
     8521 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
     8581 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
     8641 cagcggaaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
     8701 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
     8761 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
     8821 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
     8881 gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
     8941 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
     9001 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
     9061 cgctacacca attaccagcg gcggaacgaa actaccaatg aagatgcccc agtcccacat
     9121 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
     9181 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
     9241 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
     9301 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
     9361 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
     9421 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
     9481 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
     9541 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
     9601 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
     9661 tgacgagttc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
     9721 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
     9781 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
     9841 cgcgatgatt gccagaagca ggaagccaca cgccaccatg atacggaacg caaagtacag
     9901 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
     9961 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    10021 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    10081 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    10141 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    10201 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacagagtaa aggcagcagg
    10261 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    10321 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    10381 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    10441 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    10501 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    10561 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaac
    10621 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    10681 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    10741 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    10801 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    10861 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    10921 tttgccggag aggacgtaga ccgtttccat aatggccagc aggaacgcca taccgagcgt
    10981 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    11041 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    11101 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    11161 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    11221 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    11281 aatttgcatt tttcgacggt gatcaattta tagctaaatt accgcctttc agccaatttg
    11341 atcgagaaca atttatctct ttttgatgcc aatttccaag acttatacat tgataaatat
    11401 caattcctac aaattatgtt aatcatgttt caataaggta actttagtga catttatgtt
    11461 taaaatgtgt gagttataag gctgaaatta gtttcggttg gcaatttaaa gataaaaaga
    11521 gacgaagcaa aaacagcacg gttgacctta attctttttt tggtcactgg ctaatacgac
    11581 gcagagtttc aggattacta ataaaggcat aaatccaccc cgccgatatt aacatttaat
    11641 taacttgtaa ttatattata attaactcac ccagggttat tattttgtta aaaataaagc
    11701 cacatatacg tggcttgaga gtgaagataa acgcaaaatt acctgatgcg ctacgcttgc
    11761 cagtgtctac atcatctctg caatatattg aatttgcctg cttttgtaga ccgaataaag
    11821 cgttcatgcc gtatccggca tgaacaaagc gccctttgtc aacaatctgg ccgcgcatat
    11881 gtggcttcgg tttgattgct attcaggcaa gccggtaact gaacgtccgt gactggccgg
    11941 gtaaaaaagg ccccgataga tttgaacctt gattttcctc ggtggtaatg tgttcatcca
    12001 tcatcgtttc tgagcaagaa atcacctcgc gactgaaagc aacagtcgca tcacaggttg
    12061 ctgattcagc cggattaaac agccgtaaaa tcacttcttg tcggtcttca gctttcttaa
    12121 gtgcgcttat caggcatccc actgggggca ttttcaacaa actataactt tccggcacgt
    12181 tgaatccggc tttgttgagc ttcatcgcat cccatgggat tttgttgtaa cactgtactg
    12241 gagtcagcca tgctcgcgcc tgctgagcta caccagcggc ggttggcgta ccggtataac
    12301 tcaataaact taggcgacaa gaaagcagac cacgtagttg tgagtccggg actggcattt
    12361 taattcccga aggccgccca ggccttaaaa gcagatcttc tttgcccagt aagcccacgc
    12421 cacgcagcaa cgtaatggca aaggttttct tctcttcacc gatgacttca aattcacgta
    12481 acccttcgct aaagacagcc atgccgttac gcccttcctg taaggcaaca tagttgagca
    12541 tattccatac cggaaccggc gcttctttcc agccttcttg ctgccagttg ttcattgcac
    12601 tgtcgttcac ggggcgcgtt agcgaaccaa actgcgtatc tgccagaaca ctgtcagtgt
    12661 taaaaggtgt agggaccagg acacgaaggc gatgatcgtc agcctggtta tcaaggttga
    12721 tatccacatc aatacgcctg ctgttatgac taagagtgac aaccaacacc ccccctactc
    12781 tgccagtgga ttgccgggcg ctgcgttctg acaaattgag cggcactgcc atgtcatagc
    12841 ggataacagc cctgctctgc caggcttcat gaataatatc gcattgcggt ttcgcgttcg
    12901 ctgcggtaat tacccactct tcttttgcgg gtgaatagtc atattcatca ccatcatcag
    12961 agctttcttc aatttgcaat acccggtcat agcgcacacc gctgtcttta tctaccagtt
    13021 gcagagaacc atcctcattg agcgcaatct gccagaaagc attttccaga atcccttcag
    13081 cgtcactttt tgccgcaatc acgttgccag gctgattcgc ttcaatataa agcgtgcgat
    13141 agcccataga agggacaatc tggttgatct gaatatcaaa ctccataaag ggatcataat
    13201 tgccataatg aacaatttgc cgatcgatta ggccaggatc gatctcacgg gcatggcgaa
    13261 taaaatacgg tacaggctga ccgcgatcgt cttgtagatt aaactggctg gcgcgcagcc
    13321 gcacagtggt gttaataact tcttcccgcg gccagggcat caggttaaac agcacgagtt
    13381 tgtcggcatc actctgtggc atgttatcgg caattttgcg catgtagaaa cgcagcagat
    13441 tatccgccat gtcttcagcc agttcgaagc gggcgacaat ttcgcgatga actttgtcac
    13501 tgcaacaaca accgatactg tcgtgggcat gattttttaa gatctctttc cacattttct
    13561 ccagcaagcc gtggtggtat tcaaaaccca acgtccaggc cagcgttgcc agcggttcca
    13621 gcagattaac aatcttattt tcaatacgtg cgtgggcaat tttgatatcc atgcgcgtgg
    13681 aaccgatggt gcgatgtacg cgcatatatt tgccatcgat aaattcacct ttcagggtgg
    13741 ccagactttc acgctgcgct tcgatcttct caaatacctc ttcaaagcgg ctcatcacaa
    13801 acttacgttg agggtagatc tcacgtagct tatccatcac ttcgaagata ttttgctgca
    13861 atggcatctg gtcatgacca ttcggcagca aaatctcttt ggttacagac gctttttcca
    13921 gcacgtcaaa ataactgtcg aggcgtttac gtaatccgtt ttcgtcggca ggtaagtact
    13981 taccgatggc gtagcccagc ggcagcacct gcgccgtcac ttcgctaccg tcactgcttt
    14041 gccacaaaaa ctcggtttta tcagtaccgt ggcgctccga acatccgcgc cagaacatag
    14101 tgcgggtaat gccaaatcca ttgtaaatat gcggcagttg cccggacatg ccaaaggaat
    14161 ccggtaaata acctattttc atcggctcgc caaacgcgag gcagtcacgc attccgtaca
    14221 tcagattacg gacgatggat tccgcagaaa caatcgtggt atcggtctgg gtataccagg
    14281 ggccgataat caacttaccg gcttctacct gtttcttcac gcggtctttg ttttccggtt
    14341 tcaccgcgaa ataatcttcg aggatcgccg tttgcccgtc gagtacgtaa tatttgtatt
    14401 cgttgtcctg ttccagtcgg cacaggatct cttccatatt attgaccagc agaatgcgtg
    14461 actcttcggt ggtgaaatac cactcccgat cccaatgcat atgcggggtg atgtgaacgc
    14521 gagataccgc tttcatcgtc gtttcctttt tgtttatggc attacgccat cagtcagata
    14581 gttgccatgc ttaaccgcgt gacgccgcca gatcaggaga attgcagtcg agattgcagc
    14641 ccccaccaat gccgcaccaa accagccaat tgccgccata acaccgcccg cgccattatc
    14701 atgaagtaaa aagagcgaga aaatgccggc accgggtgtc gaaaggccga tattcatcgc
    14761 accgacaata gcgcccgtta ccatagagcc cagcacaaac gaaccaataa cccgcagcgg
    14821 atcttcaatc gccatcggga tcgccccttc ggtaatacct gccagcccta acagccaggt
    14881 ggatttcccg gtctcaattt caaactcttt aaacaggcgc ggtgcgagca tcgtggaagc
    14941 ggttacggta aatgccgaaa ccattttgac ggaggcgaaa atggcatacg ggccgtaaac
    15001 gccgttcgcc attgcgccca ggcagaatgc ataagcggct ttgttcactg gccctccaag
    15061 gtcaaaggaa cacataaaac cgagaatggc acccagcaac agcgcgttac ttcctgacag
    15121 accgttcagc caggcggtaa gcgagttatt gatccatgcg acaggttctc ccaccacaaa
    15181 cagcatcaga ctgcccgctc ccaacgtacc gagcaccggg tagagataaa aagtcaggaa
    15241 tccattgaat ttactgctaa gacgcaagtg atttttcacc cagcgcatca agtaaccggc
    15301 tatcaatccg ccaacgaccg cgccgagaaa cccggagccg atcatgttgg cggcaagtcc
    15361 agccgcaaag cctggcgcta acgccggttt atctgccaga gaataggcgg tataggccgc
    15421 gagcaccggt accatcaaaa ttccgagcag cccgccgccc agcttgcggt acatccacag
    15481 ccaggagttt tcttcattaa acagatcttg tagcccgaag atttgcgaca gtaataccgc
    15541 gaccgccagc accgtgcccc ccgcgacaat caacggtacg gcaaaagaga ttccgctcaa
    15601 cagtgcctgt ttcagctccg ttttgacact tttcaccggt tgcgtatctt gctgtacggt
    15661 acgcgtctca tcgctacgct tgagggtaag cgcttgttgg atcaacgctt ctgcatggcg
    15721 aatcggctcg gcaacaggca ctgaaagcgc gggaatgcca ttaaaacgct cactctcctt
    15781 gatggcgact tcagccgcaa aaatacaggc ggttgcacta ttgagttgat ccgccgttaa
    15841 acgcccttca atgccgttag cgccttgttt ttcaacgtaa acatttacgc cgagtttgcg
    15901 tccggctttt tccagatatt ccgcagccat ataggtgtga gcaatacccg ccggacaggc
    15961 cgttacgcag acgatagttg gcgcgttgga aaaagaggca gaaggttgcg tgcctccctt
    16021 gtcatccagc gccgagagca actcatcagg cgtcgtcgcc gactgtatac gcgcccgaat
    16081 ctcatcatcc gcaaggcgcg tggtcagcgc tgtcagcagt tgcatatgcg tagtacccgc
    16141 ttcattgggg ggaatcgcca gcagcaccac taaatcaact gcttccgggc catcaacgcc
    16201 ttcccactga agcggctcgc tgagtgtggc gaccgcaaac gccgcttctt ttaccgcagc
    16261 agttttgcca tgcggcacag ccaacccttc acctaaggcg gtcgggccaa ggctttcacg
    16321 gcgatacact tcttccagaa attgctcagt actggaaatt ttccccagag cagcaagacg
    16381 ttgagtcaac gcgtggatgg cctcttcacg gctggtaaag cgcgcattca gacacaacgc
    16441 atcgcggtgg gtcagagtcg tcagattcat catgatcctc acttgatcgt ttttatagtc
    16501 gcgccagtgt gcccgataaa acaataccat catgtgatca atttcacttg attgaaatta
    16561 tttgtattgt atttgtatta tttacaggat gaatttatcg ggcataatgg ctaaattcgt
    16621 attaatgaga tactaaaaat gggacacaag cccttatacc ggcagattgc cgatcgcatt
    16681 cgtgaacaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacccac tgaatcagcg
    16741 ctacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg ccagctagtc
    16801 gagcagcaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga agagcgggtc
    16861 aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg tcacgtcgat
    16921 acacacagtg aaatcctgat atttgaagtg attccggctg acgattttct tcagcaacag
    16981 ctacaaatca ccgcgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta tcgcaagcaa
    17041 aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga tctcacctgg
    17101 caggtcatgg aaaattcgaa atatcacttt attgaagaag tgaagaagat ggttatcgat
    17161 cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg tctgcttaat
    17221 atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt tgatggtcgg
    17281 gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac cctgatagcg
    17341 cagcgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca accatgtcga
    17401 aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt cttattacag
    17461 atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg cgaacggttt
    17521 tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc ccaccagcaa
    17581 tgatcgcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca gcgatgtaac
    17641 ctacaactgg cttggtaacg tgctctttga tgtacgctgc cgcttcttct tcagcgctac
    17701 cgccgatctc accgatcatc acgatcgctt cggtctgcgg atctttttcg aacatttcga
    17761 ggatgtcgat aaagttagag cctgggatcg ggtcaccgcc gataccaaca caggtcgact
    17821 gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta ccggaacggg
    17881 aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg attttgcatt
    17941 cacccggagt gataacgccc gggcagttcg ggccgatcat acgaacgccg gcttcgtcca
    18001 gtttcacttt cacagtcagc atatccagcg tcgggatgcc ttcagtgatg gtgataatca
    18061 gtttgatgcc tgcatcaata gcttccagaa tggagtcttt gcagaacggt gctggtacgt
    18121 agataacaga agcggtagcg ccagtagcag caacagcttc acgcacggtg ttgaacaccg
    18181 gcaggccgag gtgggtagta ccgcctttac ctggggttac gccgccaacc attttagtgc
    18241 cgtatgcaat ggcctgttct gagtggaaag tcccctggct accagtaaag ccctggcaga
    18301 taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg cggcaacaac
    18361 ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc tgtcagccag
    18421 tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg gtacgttaac
    18481 acccacttct gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac gaacgatacc
    18541 gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga ttttgaacgc
    18601 ttcggttaca cgttctttgg ttgcgccgcc gccaacgtca aggaagttag ccggttcgcc
    18661 gccgtgcagt ttaacgatgt ccatcgtacc catcgccaga cctgcgccgt taaccataca
    18721 accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct gtgcttcacg
    18781 cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga acagtgcgtt
    18841 gccgtcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg tgatgaccag
    18901 cgggttgatt tcgatcaacg ccaggtcgcg ctccaggaaa atggtcgcca ggcccatgaa
    18961 gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg ccagctcgcg
    19021 tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttgtgga tcaggtgcgg
    19081 agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca taaagaccac
    19141 acgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga tatcggtcgc
    19201 tgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat acgttaccag
    19261 acgcttgccg agccagtttt ctgcaaaagc acggatgtct tctttgctgt ttacaacttt
    19321 cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta cccacgggcc
    19381 ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg cataacccac
    19441 cggtgctggt aagccatagc gggcaaaaag ttgttttgcc tgatattcat gtaagttcat
    19501 gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac accctatcca
    19561 cgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca gacgcgtcgg
    19621 atcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac catcgatcag
    19681 acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct gaccattcac
    19741 cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg gcgggttgat
    19801 gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga agttaccacc
    19861 ggtcagatct tcaaccgtca gcttgccgtc acggccttta actgccagct ctttgatttt
    19921 cttctcgatg tctgccatgc cgagggtatc gacatcacgc agtaccggcg tcaccaggcc
    19981 gcgcggcgtg gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca cgtcatcacc
    20041 gtcgatagaa gcattcactt ccgggtaacg tttcagggct tcaaccaccg ctttcacgta
    20101 gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac cgtactgctt
    20161 acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca tggcggtgga
    20221 gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca tcgggacacg
    20281 tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg ccggagcaga
    20341 ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca gacgaccacc
    20401 cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca ggcgacggat
    20461 cgccgggctt aacgcatcgt tgttttgctc ttccagagac gcctgctggc gttgcgccgg
    20521 agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt tgccttcacg
    20581 caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca gaaccgcatc
    20641 cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt cgatttctac
    20701 cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg cgacggtggc
    20761 atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca ttgtgtatcc
    20821 tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt ctggtgaacg
    20881 gacatatacc ctaccgccgg agaggcggag gccgtgcggc ctgcataacg cagagaagcc
    20941 ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc ctggttgagc
    21001 ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa cacttcctgc
    21061 atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac atcgtgttga
    21121 ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca catcactacg
    21181 cgcttcacgc ccttcggatc aagctcgtcg atttcaccga tggctggcag gaaggtgccg
    21241 ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga tttcggcgac
    21301 atcactacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat gtggtaaacc
    21361 tgtgccgggg tagacggaac gcaaacctgc atgttttgct cagcacaaag ttgcagataa
    21421 cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg cggcagcaac
    21481 atcaccagac cacacatccg gccccatttc tgttcgccag aagagatgaa ctggtcgata
    21541 accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt cagagtgcgt
    21601 ggttctgcgg tggcataacc gtattcaaac gccagcactg cttcttcaga cagtacggag
    21661 tcccagacgc ggaacgcgcc ctgcccgtta tggatgtgtt gcaacggcgt gtaagtggaa
    21721 ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg accggagtct
    21781 tcacccgaca ggcgaaccgg aatgccttcg tcaaccagcg tggcgtaagc gaggttttcc
    21841 gcaccgcccc agtcgaacag tttctcaccg gcagccatcg cctggcgatc gccataaatc
    21901 ttggcaacgc gagactgcat ttcaactgct tccggcaccg tgctgatgcg tttcgccagc
    21961 tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc gtggttgagg
    22021 tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac tacgcaatcg
    22081 ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc cagcgtcgcc
    22141 actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg ttttttgatt
    22201 ttctgataca tcagcggctg ggttgcgctc ggctcgtcgg cttcgttgtg gccgtgacgg
    22261 cggtagcaca ccaggtcgat gaagacatca cgtttaaagg tgttacggaa atcgagcgcc
    22321 agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa aatcggtgcc
    22381 tgaaccatct taccgatatc agtacagtac ggtgtagaac gagcatccag tggattagag
    22441 gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac ttcataacca
    22501 cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac tgcggcgtca
    22561 ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct gtccagacgg
    22621 gcacgaacag aaccgataac taccgggctt acaatctcaa ggtgagacgg gttaaacgcc
    22681 agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc catgtggtat
    22741 ttcacgtcac ccgtgccgag gtgttcttta tgtttaccgg caaactcgtc gaacaagtct
    22801 tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg cgccatccca
    22861 agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt aagcatcggg
    22921 attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt tgcgccgagg
    22981 taacgttcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt ctcttcgcta
    23041 ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc ttcggtgctg
    23101 gtaatgtgca tatactcggc accaatcggg ccacagtagg tttgcttgag ggcttccagc
    23161 agctcgccga gtttcatgtt ttctttgccg ctggcaaatg aaccgacgtt gaaggtctcc
    23221 tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac tttatcttgc
    23281 tgccacagtc ccagcggatc gagattcgca tgctggtgac cacggaagcg gtatgcgtta
    23341 atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat cgttgaagag
    23401 taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga gtggaattga
    23461 tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt agcgtcaacc
    23521 gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg gtttgcgcca
    23581 gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt gatcccttaa
    23641 gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg tgcgtgccgg
    23701 ggttcacttg cgagctcttg tctatgtagt ggctcgcgaa ggaaccttta aaaactgtct
    23761 tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct gatgcgctgc
    23821 gcttatcagg cctacgggta atgacttgta ggccggataa ggcgttcacg ccgcatccgg
    23881 cactggttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacggttta cgcattacgt
    23941 tgcaacaaca tcgacttaat atggccgatg gcgcgcgtcg ggttcagccc cttcggacat
    24001 acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc gctcaaaccg
    24061 tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata tgccgctaac
    24121 aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca agaggttgaa
    24181 caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg ctctggcatc
    24241 tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg cttaattttc
    24301 tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac cggtaaacct
    24361 ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat cggggtaata
    24421 caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac accttcacgg
    24481 caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag cgcatccagc
    24541 agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg cggagcatca
    24601 tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct gtctccgcat
    24661 tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat gttgacgctt
    24721 cggcgcgtca tggattccga ctctggcaga tacagggagt ggcacagcca gttttcatca
    24781 tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg gaagttggca
    24841 gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca ctcaacgcgc
    24901 tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg ctcgcggatc
    24961 actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac cgagaagtta
    25021 tgctgcatac attcttgcag cgctttacga atcgccaccg gatcttcacc gttacgatta
    25081 ttgttccagc ggttcagacg atccagagat gcttcaacat cagactcgct ggcatcgcgc
    25141 aatgcgccct gttcggcgat agactcttgc agatgcagac ctgccgcacg accaaagacc
    25201 accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga tacacaagcg
    25261 atttcaccaa cggcaaacag ccccggaaca accacatctt cgcctttctc gttcaccgtc
    25321 agcgcctgac cggtcacttt ggtcggaata ccgcccatca tgtagtgaca ggttgggata
    25381 accggaatcg gctctttcac cggatcgacg tgagcaaagg tacgggaaag ctccaggata
    25441 cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag tttcgcgtgt
    25501 ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat ggaacgcgcc
    25561 accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat aaagcgttcg
    25621 ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac cagtacgccc
    25681 gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac cggtacgcct
    25741 gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt ggtggactga
    25801 taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa ataaaccact
    25861 tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg gtttttcacc
    25921 agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt ctgctgataa
    25981 agcgtgtgca acagtgcgtg accggtacgg tcagccgccg ccgcagtgcg tgccgcctgc
    26041 tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg accatcatca
    26101 agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg cccggtttta
    26161 cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac ggtgtcgtac
    26221 atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat gccgccttgc
    26281 gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca ggtctggccg
    26341 ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat cacaactgca
    26401 tcaaattctc tgactggcaa tttcatcaca caccccacac cacaacgaat ccataaatca
    26461 cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaat gccagcggtt
    26521 taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag atagaaaaca
    26581 gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc cagacttcat
    26641 atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag agcgtcagga
    26701 cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct aatgcggagg
    26761 cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa taacaaagga
    26821 gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa aatccatcat
    26881 catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc cccacatgat
    26941 aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt caggggaaga
    27001 gaggctggta cccagaagcc acagcaggat gcccactgca acaaaggtga tcacaccgga
    27061 aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct gtaggtccag
    27121 attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca tgctgttctt
    27181 attattccct ggggactacg ggcacagagg ttaactttct gttacctgga gacgtcggga
    27241 tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttctataact gcgcgtcatg
    27301 caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc tcgggattgc
    27361 agggtgttcc ggagacctgg cggcagtata ggctgttcac aaaatcatta caattaacct
    27421 acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa caacatttat
    27481 ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa cattgccagg
    27541 aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt aacttatgta
    27601 acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga caagtttaat
    27661 aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat cctttacctg
    27721 caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc gagccaaata
    27781 acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc agtcttacgc
    27841 aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac cctcaacggg
    27901 gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt tattgatatc
    27961 cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc aaccgcatcc
    28021 tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca ccgcggtttc
    28081 ccgatcgatc agctggcgac cgattctaac tacctggaag tttgttacat cctgctgaat
    28141 ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac ccgtcatacc
    28201 atgatccacg agcagattac ccgtctgttc catgctttcc gtcgcgactc gcatccaatg
    28261 gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc gctggatgtt
    28321 aacaatcctc gtcaccgtga aattgccgcg ttccgcctgc tgtcgaaaat gccgaccatg
    28381 gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg caacgatctc
    28441 tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc gtatgaagtt
    28501 aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga ccatgaacag
    28561 aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc gtttgcctgt
    28621 atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa cgaagcggcg
    28681 ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt tcgtcgtgcg
    28741 aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta caaaaattac
    28801 gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga gctgggcacc
    28861 aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa cgacccgtac
    28921 tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat cctgaaagcg
    28981 atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac cgttggctgg
    29041 atcgcccact ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc gcgtcagctg
    29101 tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg gttgattgct
    29161 aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata tgcccaaaca
    29221 caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa aaaaatatat
    29281 cggtataaat tataaacaaa tcgatgat
//
LOCUS       DAZOYS010000030        28356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOYS010000030 DAZOYS010000000
VERSION     DAZOYS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.030"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1170
                     /locus_tag="ACHUN9_001180"
     CDS             <1..1170
                     /locus_tag="ACHUN9_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0694636.1"
                     /translation="SYKPIWNWKISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTS
                     TRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQ
                     LDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIID
                     LLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYK
                     IMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNS
                     RAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGL
                     NARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHUN9_001181"
     CDS             1360..1473
                     /gene="yhiY"
                     /locus_tag="ACHUN9_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0694637.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1536..2603
                     /locus_tag="ACHUN9_001182"
     CDS             1536..2603
                     /locus_tag="ACHUN9_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0694638.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHUN9_001183"
     CDS             2600..5335
                     /gene="rbbA"
                     /locus_tag="ACHUN9_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0694639.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5335..6459
                     /locus_tag="ACHUN9_001184"
     CDS             5335..6459
                     /locus_tag="ACHUN9_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0694640.1"
                     /translation="MRHLRNIFNLGIKELHSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6532..6805
                     /locus_tag="ACHUN9_001185"
                     /pseudo
     CDS             6532..6805
                     /locus_tag="ACHUN9_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6802..7161
                     /locus_tag="ACHUN9_001186"
     CDS             6802..7161
                     /locus_tag="ACHUN9_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0694641.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7281..7682)
                     /gene="nikR"
                     /locus_tag="ACHUN9_001187"
     CDS             complement(7281..7682)
                     /gene="nikR"
                     /locus_tag="ACHUN9_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0694642.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7688..8494)
                     /gene="nikE"
                     /locus_tag="ACHUN9_001188"
     CDS             complement(7688..8494)
                     /gene="nikE"
                     /locus_tag="ACHUN9_001188"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0694643.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8491..9255)
                     /gene="nikD"
                     /locus_tag="ACHUN9_001189"
     CDS             complement(8491..9255)
                     /gene="nikD"
                     /locus_tag="ACHUN9_001189"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0694644.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(9255..10088)
                     /gene="nikC"
                     /locus_tag="ACHUN9_001190"
     CDS             complement(9255..10088)
                     /gene="nikC"
                     /locus_tag="ACHUN9_001190"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0694645.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10085..11029)
                     /gene="nikB"
                     /locus_tag="ACHUN9_001191"
     CDS             complement(10085..11029)
                     /gene="nikB"
                     /locus_tag="ACHUN9_001191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0694646.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11029..12603)
                     /gene="nikA"
                     /locus_tag="ACHUN9_001192"
     CDS             complement(11029..12603)
                     /gene="nikA"
                     /locus_tag="ACHUN9_001192"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0694647.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12714..13301)
                     /gene="acpT"
                     /locus_tag="ACHUN9_001193"
     CDS             complement(12714..13301)
                     /gene="acpT"
                     /locus_tag="ACHUN9_001193"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0694648.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13356..14405)
                     /locus_tag="ACHUN9_001194"
     CDS             complement(13356..14405)
                     /locus_tag="ACHUN9_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0694649.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14537..15754
                     /locus_tag="ACHUN9_001195"
     CDS             14537..15754
                     /locus_tag="ACHUN9_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694650.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMLWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15758..16315)
                     /gene="dcrB"
                     /locus_tag="ACHUN9_001196"
     CDS             complement(15758..16315)
                     /gene="dcrB"
                     /locus_tag="ACHUN9_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0694651.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16388..17053)
                     /locus_tag="ACHUN9_001197"
     CDS             complement(16388..17053)
                     /locus_tag="ACHUN9_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0694652.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            17274..17519
                     /gene="tusA"
                     /locus_tag="ACHUN9_001198"
     CDS             17274..17519
                     /gene="tusA"
                     /locus_tag="ACHUN9_001198"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0694653.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17621..19819)
                     /gene="zntA"
                     /locus_tag="ACHUN9_001199"
     CDS             complement(17621..19819)
                     /gene="zntA"
                     /locus_tag="ACHUN9_001199"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0694654.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19893..20519)
                     /locus_tag="ACHUN9_001200"
     CDS             complement(19893..20519)
                     /locus_tag="ACHUN9_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0694655.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20660..21019
                     /locus_tag="ACHUN9_001201"
     CDS             20660..21019
                     /locus_tag="ACHUN9_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0694656.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21022..21291)
                     /locus_tag="ACHUN9_001202"
     CDS             complement(21022..21291)
                     /locus_tag="ACHUN9_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0694657.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21281..21877)
                     /gene="rsmD"
                     /locus_tag="ACHUN9_001203"
     CDS             complement(21281..21877)
                     /gene="rsmD"
                     /locus_tag="ACHUN9_001203"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0694658.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22027..23523
                     /gene="ftsY"
                     /locus_tag="ACHUN9_001204"
     CDS             22027..23523
                     /gene="ftsY"
                     /locus_tag="ACHUN9_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0694659.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23526..24194
                     /gene="ftsE"
                     /locus_tag="ACHUN9_001205"
     CDS             23526..24194
                     /gene="ftsE"
                     /locus_tag="ACHUN9_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0694660.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            24187..25245
                     /gene="ftsX"
                     /locus_tag="ACHUN9_001206"
     CDS             24187..25245
                     /gene="ftsX"
                     /locus_tag="ACHUN9_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0694661.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25490..26344
                     /gene="rpoH"
                     /locus_tag="ACHUN9_001207"
     CDS             25490..26344
                     /gene="rpoH"
                     /locus_tag="ACHUN9_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0694662.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26615..27718
                     /gene="livJ"
                     /locus_tag="ACHUN9_001208"
     CDS             26615..27718
                     /gene="livJ"
                     /locus_tag="ACHUN9_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0694663.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27917..28300)
                     /gene="panM"
                     /locus_tag="ACHUN9_001209"
     CDS             complement(27917..28300)
                     /gene="panM"
                     /locus_tag="ACHUN9_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0694664.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6761 a         7347 c         7836 g         6412 t
ORIGIN      
        1 tcgtacaaac ccatctggaa ctggaaaata tcgcaataca atgtatttga tattaaattc
       61 gaaacaatga taaaacccca gttcgctgac agaacctatt tctccaattg ttcacctgtt
      121 gatcccacaa gtaccagacc gacctatttt ggcgataccg atggctcagt cggtgcagtt
      181 ttgtacgcgc tttttgcaac gggacatttg ggaatcatgg ctgaaggaga aaatttcctt
      241 agccagttgc ttaatataga agatgaagtt ctcaacgtat tgctaaggga gaactttaat
      301 gagcaacttg acactaatgt taatataata atcagtatcc tgaacagaag agacattgtt
      361 ttagaaagtc ttcagcctta tttggttatt aataaagacg cagtcacgcc atgtacgttt
      421 ctgggcgacc agactggcga tcgatttagt aatatctgtg gcgataaatt tatcatcgat
      481 ttattaaaac gcataatgag tatcaatgaa aacgttcatg tattagctgg aaatcatgaa
      541 acgaattgca atggtaacta tatgcagaac ttcacgcgca tgaaaccact tgatgaggat
      601 acctactctg ggataaaaga ttatcctgta tgtttctatg accccaaata caaaataatg
      661 gcaaatcatc atgggataac ttttgatgat cagcgaaaac gttacattat aggaccgata
      721 acagtctcta gtgatgaaat gaccaatgct ctcgatccgg tcgaattagc ggcaattatc
      781 aataaaaagc accatgctgt aattaatggc aaaaagttta aaaatagccg ggctatctct
      841 tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt acagacctaa acttgaagca
      901 ctattagcat gctcacaaat gttaggtata aatcaagttg ttgcacataa cggtaatggt
      961 ggaagagaat gtataggtga aacggggaca gttttaggtc ttaacgcccg tgacagtaaa
     1021 catgctggaa gaatgttcag catgcataat tgccaaatta accctggtgc agggcctgag
     1081 ataaccactc cctggaagtc ttaccagcat gaaaaaaaca gaaatggact tatgcctttg
     1141 atcagaaggc gcacaatgtt acagctctga accatagaca acatgaatat taatgttact
     1201 atctggttga gactgttgat aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag
     1261 gcgtgaaacg ctatccaaca ggatggatac cgttttgtca gccagtttta tagatcacac
     1321 ttatgagtat gcgtgtttca tttacggcca ggaatgctga tgatgacaac attactaccc
     1381 gttttcacta agccttcccc gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt
     1441 ttccttctta tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg
     1501 cgtcgtttgc ttgcagggat tatggcgggt cgattatgga taagagtaag cgccatctgg
     1561 cgtggtgggt tgtcgggtta ctggtggtgg ctgccgtcgt ggcgtggtgg ctgttgcgcc
     1621 cggcgggtgt gccggaaggc tttgctgtca gcaatgggcg cattgaagcg acggaagtgg
     1681 atatcgccag caaaattgcc gggcgtatcg acaccattct ggtgaaagaa ggccagtttg
     1741 ttcgcgaagg cgaagtgctg gcgaagatgg atactcgcgt gttgcaggag cagcgactgg
     1801 aagccatcgc gcaaatcaaa gaagcacaaa gtgccgttgc tgccgcgcag gctttgctgg
     1861 agcaacggca aagcgaaact cgtgccgcgc agtcgctggt taatcaacgc cagtccgaac
     1921 tggactccgt ggctaaacgt catacgcgtt cccgctcgct cgcccaacga ggggctattt
     1981 ctgcgcaaca gctggatgac gatcgcgccg ccgctgagag cgcccgagct gcgctggaat
     2041 cggcaaaagc tcaggtatcg gcttctaaag cggctataga agcggcccgt accaatatca
     2101 ttcaggccca aactcgcgtc gaagccgcgc aagccactga acggcgtatt gccgcagata
     2161 tcgatgacag tgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg gttgctgagc
     2221 caggggaagt gctggcggca ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct
     2281 atatgacttt cttcctgcca accgaacagg cgggcacgct gaaacttggc ggtgaagccc
     2341 ggctgatcct cgatgccgcg ccagatctgc gtattccggc gaccatcagt tttgtcgcca
     2401 gtgtcgccca gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt
     2461 tccgcgtcaa agcgcgtatc ccaccggaat tactccagca gcatctggaa tatgtcaaaa
     2521 ccggtttacc gggcgtagcg tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc
     2581 tcgtggtgag gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca
     2641 actggcgggc gtgagccagc attatggaaa aaacgttgcg ctgaacaata ttactctcga
     2701 tattccggcc cgctgtatgg tcgggctgat tggtccggat ggcgtcggga agtcgagctt
     2761 attgtcgttg atttccggtg cccgcgtcat tgagcagggc aatgtgatgg tgctgggcgg
     2821 cgatatgcgc gacccgaggc atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca
     2881 ggggctgggc aaaaacctct accacacctt gtcggtgtat gaaaacgtcg attttttcgc
     2941 tcgcctgttc ggtcacgaca aagcggagcg ggaagtgcga attaatgagc tgctgaccag
     3001 cacggggtta gcaccgtttc gcgatcgtcc ggcagggaaa ctctccggcg ggatgaagca
     3061 aaaacttggg ctgtgctgcg cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc
     3121 aacaacgggg gttgacccgc tctcccgctc ccagttctgg gatctgatcg acagtattcg
     3181 ccagcggcag agcaatatga gcgtgctggt cgccaccgcc tatatggaag aggccgaacg
     3241 cttcgactgg ctggtagcga tgaatgccgg agaagtgctg gcaactggca gcgccgaaga
     3301 gctacggcag caaacgcaaa gcgctacgct ggaagaagca tttataaatc tgttaccgca
     3361 agcgcaacgc caggcgcatc aggcggtagt gatcccaccg tatcaacctg aaaacgcaga
     3421 gattgccatc gaagcgcgcg atctgaccat gcgttttggt tccttcgttg ccgttgatca
     3481 cgttaatttc cgcattccac gcggggagat ttttggtttt cttggttcga acggctgcgg
     3541 taaatccacc accatgaaaa tgctcaccgg actgctgccc gccagcgaag gtgaggcgtg
     3601 gctgttcggg caaccggttg atccaaaaga tatcgatacc cgccgtcggg tgggctatat
     3661 gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg caaaaccttg agttacatgc
     3721 ccgtttgttt cacatcccgg aagcggaaat tcccgcaaga gtggctgaaa tgagcgagcg
     3781 ttttaagctc aacgacgttg aagatgttct gccggagtca ttgccgctcg gcattcgcca
     3841 gcggctttcg ctggcggtgg cggtgattca tcgcccggag atgttaatcc tcgatgagcc
     3901 tacttctggt gtcgatccgg tggcgaggga tatgttctgg cagttgatgg tcgatctctc
     3961 gcgccaggac aaagtgacta tcttcatctc tacccacttt atgaacgaag cggaacgttg
     4021 cgaccgcatc tcactgatgc acgccggaaa agtgcttgcc agcggtacac cgcaggaact
     4081 ggttgagaaa cgcggagccg ccagtctgga agaggcattt atcgcctatt tgcaggaagc
     4141 ggcagggcag agcaacgaag ccgaagcgcc gcccgtggta cacgacacca cccacgcgcc
     4201 gcgtcaggga tttagcctgc gccgtctgtt tagctacagc cgccgcgaag cgctggaact
     4261 gcgacgcgat ccagtacgtt cgacgctggc gctgatggga acggtgatcc tgatgctgat
     4321 aatgggttac ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga
     4381 ccagaccgtc agtagccagg cgtggacact caacctctcc ggttcccgtt actttatcga
     4441 acagccgccg ctcaccagtt atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac
     4501 ggtggcgatt gagatcccgc ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga
     4561 actcggcgtc tggatcgacg gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt
     4621 gcaggcaatg caccagagtt ggcttcagga tgtggcgagc cgacaatcga cacccgccag
     4681 ccaaagcggg ctgatgaaca ttgagacgcg ctatcgctat aacccggacg taaaaagcct
     4741 gccagcgatt gttccggcgg tgatcccgct tctgctgatg atgatcccgt caatgctaag
     4801 cgcccttagc gtggtgcggg aaaaagagct agggtcgatt atcaaccttt acgtgacccc
     4861 caccacccgt agcgaatttt tgcttggcaa acagctgccg tacatcgcgc tggggatgct
     4921 gaactttttc ctgctctgcg gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag
     4981 tttcctgacg ctcaccctgg cggcgctgct gtatatcatc attgccaccg gaatggggct
     5041 gctgatctcc acctttatga aaagccagat tgccgccatt ttcggaacgg cgattatcac
     5101 gttgatcccg gcgacacagt tttccgggat gatcgatccg gtagcttcgc tggaagggcc
     5161 tggacgttgg atcggcgagg tttacccgac cagtcatttt ctgactatcg cccgcggaac
     5221 gttctcgaaa gcgctggatc tgactgattt gtggcaactt tttatcccgt tgctgatagc
     5281 catcccgctg gtgatgggct taagcatcct gctgctgaaa aaacaggagg gatgatgcgc
     5341 catttacgca atatttttaa tctgggtatc aaagagttgc acagtctgct cggtgataaa
     5401 gcgatgctga cgctgattgt cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt
     5461 acgccaggat cgttgaacct cgcgccgatc gccattgccg atatggatca atcgcagtta
     5521 tcgaaccgga tcgttaacag cttctatcgt ccgtggtttt tgccaccgga gatgatcacc
     5581 gccgatgaga tggatgccgg actggacgcc ggacgctata ccttcgcgat aaatattccg
     5641 cctaattttc agcgtgatgt cctcgccgga cgccagccgg atattcaggt gaacgtcgat
     5701 gccacgcgca tgagccaggc atttaccggc aatgggtata tccagaatat tatcaacggt
     5761 gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg aaccgttggt atcgctggaa
     5821 acccggatgc gctttaaccc gaacctcgat cccgcgtggt ttggcggggt gatggcgatc
     5881 atcaacaaca ttaccatgct ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt
     5941 gaacacggca cggtggaaca cttactggtg atgccgataa cgccgtttga gatcatgatg
     6001 gcgaagatct ggtcgatggg gctggtggtg ctggtggtat cgggattatc gctggtgctg
     6061 atggcgaaag gtgtactggg cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc
     6121 gtggcgctca gtctgtttgc caccacgtca atcggcattt ttatggggac gatagcgcgt
     6181 tcaatgccgc aactggggct gctggtgatt ctggtgctgc tgccgctgca aatgctttcc
     6241 ggtggatcca cgccgcgcga aagtatgccg cagatggtgc aggacattat gctgaccatg
     6301 ccgacgacac actttgttag cctcgcgcag gccattctct accggggtgc cggattcgaa
     6361 atcgtctggc cgcagttcct gacgctgatg gcaattggcg gcgcgttttt caccattgcg
     6421 ctgctgcgat tcaggaagac gattgggaca atggcgtaag gaagggaaac ttgacttcat
     6481 actgtgatgt catactattg atgtcataaa gtgaagtcat aaggacatgc catgagaacc
     6541 aaggtacagg ctttacggaa gaaaaaaaaa tacattggat caaatattta aaactcctgt
     6601 tcctcaaggg atcaagtggt ctgatataga gtcactggtt aaagcattag gcggagaaat
     6661 taaggaagga agaggttcgc gttgtaagtt catactaaat atgagcgttg cgtgtttcca
     6721 tcggcctcat ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc gtgactggtt
     6781 actaagtata ggggtaaaac catgatcaaa ctgaagacgc caaactctat ggagattgcc
     6841 gggcaaccag ctgttattac ctacgtgcca gaactgaatg catttcgtgg caaattcctg
     6901 ggcttgtcag gttattgtga ttttgtctct gacagcattc aggggctgca aaaagaaggg
     6961 gaactttccc tgcgagagta tcttgaggat tgtaaagctg cgggtattga gccgtatgcg
     7021 cgtacagaaa agattaagac ctttacgtta cgctatccag aatcgctgag tgaacgcctt
     7081 aataatgcgg ctgcgcagca acaagtctct gttaatacct atattattga aacactgaat
     7141 gagcgtttga atcatttata aattttagcg gcgtacatta cgcagaatat ctgcctttac
     7201 aggatctatc tatgcaactg aacttaattt tataaattaa gttcagttaa tatcttttct
     7261 ttcctcgttt cagaaataac tcaatcttcc ttcggcaagc actgcaaatg cccatgccgc
     7321 acgccgcgct gggcgataac gtcatcggca aaatgctgca cgtcgcccat atcacctttc
     7381 agcacggcga tttccagaca gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag
     7441 agatcgtggt gatggtgctg ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt
     7501 tcatacacat aggagagcac cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg
     7561 gccagggcgc tacgcagaat atcgcggata gcttcggaac ggttgttata accacgacgc
     7621 tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc
     7681 atttgcgtta aaccttttct gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt
     7741 tttgtagcac acgtccggcg tcagaggaaa aggttaattt atctcccacc acctgggttt
     7801 cgacgatttg tccgttgtcc atcaccatca cccgctgaca gaagcgttcc accaggcgta
     7861 agtcgtgggt gatgaacagg caggcggtgc caaactgttg ttgtagcttt ttcagcaggc
     7921 gaatgacgcc cgcctgtaac acgagatcaa ggttagaaac ggcttcatcc agaatcagta
     7981 gtttcggttc gaccgccagc gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta
     8041 actgcggtgg gcgtttgtcg agaacgctgt catcgagatc gaccgccttc agcatttcgc
     8101 tggcgcgcgc cagttgttcg gattttttca gtgatagcag gtggcgcatc ggttcacgca
     8161 ggatctcgcg cacggttttg cgcggattca cggcgctgat ggagtcctga aataccatct
     8221 gaatatcgcg gcggaacgct ttacgctggg cgcgattgag tttcgccagc ggttcgccac
     8281 gccagctaat attcccctgc gcgggcgatt ctaaacccac cagcaaccgc gcgagggtac
     8341 ttttcccgca gccgctgcgc cccagcaggg cgacggtttc gccgcttttc agggtcaggg
     8401 aaacgttatt cagcaccgcc tgatgttgat gttttccgtt aaatccaccg tgggcatagt
     8461 gatgggaaag gccggagacg ttaagtaaag tcatgatgcc agctccatac cgtagagggc
     8521 gagatgagcg gaaaccaggc tgcgcgttac cgcatgtttg ggggcgttaa acagcgtttc
     8581 tacatcgccc tgttcgacaa ttttaccttg tgacatcacc gccacgtcat ccgccagacg
     8641 cgccacaacg cccatatcat gggtgaccag cagcattccc ggcgcttgtt tttgcataat
     8701 gctttccagc agatcgagga tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc
     8761 atcggcgatg ataaacggtg attcacacag caccgccatc gcaatcatca tgcgctgcaa
     8821 catgccgccg ctcatctcga acgggtacag cttcagcacg cgcgcggcgt tttccagccc
     8881 caccgcttct atggcagcgg taagcgtagc gtcatcggcg ggtttcccta acgccaggca
     8941 ggtttcccgc gcgtgggtgt gcatggtgtg tagcggatta aaggcgctgc gcgggttctg
     9001 catgatggtg gcaattttga tgccgcgcag ggcgcaggga taaaccgctt tgccatcggc
     9061 taaaatttcc cccgccgtct ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca
     9121 ggttaatgat ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag
     9181 ggttaacgat acaccgtgta ccagcggctg cgcggcctgt agcgcgatat tacgtagttc
     9241 aatctgttgc ggcattagtg tgcgtgctcc gtcaccagat gaggatccag atgatcgcgc
     9301 agtgcgtcac ccaccaggtt aaaggccatc acgctgataa acagcgccag ccccggccag
     9361 aacatttgca gcggctgggt ccagatatac tggcgcgcgt cgttaatcat cacgccccat
     9421 tcggcggtcg gcgcggtcac accgaggcca aggaaagaca tccccgcgac gtgcagcatc
     9481 atatggccga tatccagcgt tgccagcacc agcagcgaag ggatcaccgc gcctgccaga
     9541 tgatcgacaa acacccgcac atggcccgca ccggaaagcc gtgacgccag cacaaactcg
     9601 cgttggcgta gtgaaatcac caagctgcgc accatgcgtg cataccacgc ccagtgcgac
     9661 agggcgatgg cgataattac gttggtcagc ccggtgccga gcacgccaac cataaagaac
     9721 gacagaatcg aggtcgggaa ggtcataaac atatcggcga cgcgcatggt ggcctgatca
     9781 acgcgcccgc caatcaaccc ggcgctgccg ccaataacca gccctaatgt cagcaccagc
     9841 agcaggcagg ccattaccga accgagcgac acgcgggtcg ctgccatcag ccgcgagaaa
     9901 atatcgcgac ctaagtgatc ggtgcccagc cagtgctgcg catccggcga aagcaggcgc
     9961 gacggcaaat caatcgcctg ggggtcatac ggcagccacc actggctggt gagcgcaatc
    10021 agcgccagca gggcgataat gatcagcgcc aggcgtaccg accagcggga agagaggaaa
    10081 aagttcacgc gtgcgctcct tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca
    10141 aatcgacaat caaattacag accacaaaaa ccaccaccat catcagcgta aagcactgaa
    10201 tcaccggata gtcacggtta aaaatcgccg acaccgcata gcgcccgacg cccggccagg
    10261 caaagatgtt ttcgataatc atcgtcccgc caatcagttc gccgatgtgc atccccacgg
    10321 cggtgatcat cggcagcgag gcattgcgca gaatatggcg gcgttcggtc tgtttgtcgt
    10381 tcaggccgcg cagacgcgcc caagtgacgt gacgctgacc ggcgacgtcc agcatactgg
    10441 cgcgcagcag tcgcgcgtta atcgccagcg acataaaggc aatggaaacc gcaggcaaaa
    10501 tgatgtgctg ccagccgccg taacccatcg cgggtagcca ttgcagatac accgaaaacg
    10561 ccatcaccag taaaaacgcc agccagaagt taggcatcga cacgccaaga aacgcgatga
    10621 aacgtacggc gaaatccggc agacggtcgc gatggcgcgc cgcccagata ccgagcggta
    10681 cggaagtgag cagaattaat accagcgccg cacctgcaag ttccagcgtg gcgggcagga
    10741 agttcagcat atcgtccagc accgggcgtt ggctggcgaa tgagatacca aagtcaagat
    10801 gcagcgcctt ccacaaccag gtaccgtact ggacgtacag cggctgatcc agcccgagca
    10861 tggtgcgggt agaggccagc atctccggcg tcggcggcag gttagataaa cgcagataat
    10921 cgagcgccgg gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga
    10981 gcaccatcgg gatcagcagc agaaagcggc gtaatacgta acgcaacatt aaggtttcac
    11041 cggtttaatc tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga tattacccag
    11101 ctccggtttt gataccacca tcatcgagat gtagctgata ggcagataaa ccgcctcgtc
    11161 atgcagacgg gtcaaaatgt cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc
    11221 cagcacttcg ccgatctctt tatcaatcag cggcttgtcg gctaatcctt gctgtgcctg
    11281 gaagtcagcg tgcgacggta cgcgcattga actcaggaag gcgtgtggat cgtatggcgc
    11341 gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag catagatact
    11401 gctctcttct tcgccaatca gcgagacatc tgcgccaatc tggcgcatat cagcctgaat
    11461 gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag
    11521 cggctgacca tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac cggctttttc
    11581 cagcaactct ttcgctttct gcggatcgta ctggcgcggt ttcaggccga gattagcgta
    11641 aggcacagaa ggggcaaaca gggtgtcggc gacctgctgg gtgccatata acgcgttatc
    11701 aatcagcgat tttttgttta ccgcgtaatt aagagcttca cgtactgcca gctcgttggt
    11761 gggggctttg gcggtattga gcgccagcat cacggtttcg atcggctgtg acagttgggt
    11821 gtggtaagcc ggattctggc taaagcgggc gaaggtatcg agcggtaata acccttcgtt
    11881 tccgtacagc aggtcgatat cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg
    11941 gatgacgtta aaggtgatct ttttaatcgc tggcttttcg ccccagtagt tttcgttacg
    12001 gacgaagaca tcgtactgat tcagtttcga ttcctgcaaa atccacggtc cggtgccaat
    12061 cggcgcttta attccgttca tggtttcatg gtttttaaac tgcgagggag cgataaaacg
    12121 gaaaggacgg ggcagggcca gttcttgcag gaaaggatag taggcgcttt tcagggtaat
    12181 ttgcagctct gttttactga gtgctttaac atcaacaatc tggtttgcca gctccagcca
    12241 ggcgtgacgt tgacggttat cgagcactgc gcggaagttt tctgccgccg cctcggcatc
    12301 gaacggttca ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc aggttttacc
    12361 atcttctgaa tgagtccagc tttttgccag ccacgggatc accgaaccgt ctgcctgata
    12421 tttcaccaat ggttcataaa ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg
    12481 cgggtttagt ggcccgacat tcaccggcca ggcggtagtg atttcatctg gtgcagcggc
    12541 atggacgata aaagacgcac aagccagcag cgcaaataga gtgcggcgga gtgtggagag
    12601 catggtaacc ccaatggatt aaaatagatg gcggaaataa gtatgacgat tttaagtatt
    12661 cgtcatactg attacctgtt ctggatcaat agatgggcag tcgggtgggc ggatcagtta
    12721 actgaatcga tccattgcac actgtcggcg gtgagcgtaa agggggtggg ggtgcagatc
    12781 gcaaggctta aattttctag ctgacaatgg ctgactgaca gcgaggagtg ataggtgcta
    12841 tctacgctga cgatttgcca ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc
    12901 gtccagatgc gccagaacat ttccagttgc tgatcaggat gcacggcgtc catttcagcg
    12961 tgttccccga ggctgaacac ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc
    13021 acttcgatat cgcagccgac ttcgccttca tcgctcaaca gcagagcgat atcgtcaccg
    13081 ctatggctta agttgaacca tagcggcgtt tccggcgcga atgcaggttt gccttgttcg
    13141 ccatagatga tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc
    13201 cagcgttcgc gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg cagtggagct
    13261 gcgcttaagg tcgaaacttt ccccagaact atccgataca tatcagggcc aacgtttaat
    13321 ggaaaatgaa agtgcgtatc gtatcacttg tcgcctcatc ccggtaaccg acttttcggt
    13381 ctgcctggcc ccagtaaaat cgccagtttg ctaccgcctt tggtggtttc catccagatt
    13441 ttacacacac tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc
    13501 caaattaaca atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc
    13561 cgtggttcta aaatattgcc gatgaccata tggaccacta aaaacaatgc gccgaccaga
    13621 atacattcgt aaacaccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt
    13681 accgcgccga tattgggcac gtagttgagc aaaaacgcca gtactgccca catcagcgca
    13741 aactgcaccc ccatcagctc cagccccagc cagacgatga cacctgtcca taaactgagt
    13801 agcgtcttca atgcaagata gtgcgaaacg cctttaagtg cgcggtgtaa tcctgcgatg
    13861 tgaatctgtg ggttattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac
    13921 agcataaaaa ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg
    13981 gtcattagcg ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc
    14041 tccggcgaca tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc
    14101 gtcagctcct tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt
    14161 acgccgacca gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg
    14221 gggcgttgta ctccccgacg aataaaccag gtaacgagcg ggttgaggac gatggcaaaa
    14281 aacagcgcca gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc
    14341 actaccagcg aggccagctt gagcagaatg tgcatgcccg ttttatcggg ttgagggatt
    14401 tccattgggg cttccttgtg actttttgta ttaagtgtag cgggagtcac gccagcaata
    14461 ttctgattct tactgctgat tttcacgtca gcacgtggta aaaatgaaac actgttgtaa
    14521 aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct
    14581 gaatttgcgc attgtctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt
    14641 gccgctcgct gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ctttctgggc
    14701 aggattggtt atcagcctgc aatatttcgc caccttgctg agccgtcctc atgccggacg
    14761 ttacgccgat ttgctgggcc ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt
    14821 gagcggtctg ggatatctga cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt
    14881 attactttgc ctgggacgcg tgatccttgg gattgggcaa agttttgccg gaacgggatc
    14941 gaccctgtgg ggcgttggcg tggttggctc gctgcatatc gggcgagtga tttcgtggaa
    15001 cggcattgtc aattacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca
    15061 ctggggcggc ttgcaggcgt tagcgttaat cattatgggc gtggcgctgg tggccatttt
    15121 gttggcgatc ccgcgtccga cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc
    15181 ggtgcttggg cgcgtctggc tgtacggtat ggcactggca ctggcttctg ccggatttgg
    15241 cgtcatcgcc acctttatca cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt
    15301 cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat
    15361 taaccgtatc ggcggcttaa acgtggcgat gatttgcttt agcgttgaga taatcggcct
    15421 gctactggtt ggcgtggcga ctatgctgtg gatggcgaaa gtcggcgtct tactggcggg
    15481 ggcagggttt tcgctggtgt tcccggcatt gggcgtagtg gcggtaaaag cggttccgca
    15541 gcaaaatcag ggggcggcgc tggcaactta caccgtattt atggatttat cgcttggcgt
    15601 gaccggacca ctggctgggc tggtgatgag ctgggcgggc gtaccggtga tttatctggc
    15661 ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc
    15721 ggaacacgtc cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa
    15781 tgatgttttc tgcggtggtc tgcgcttttt gctgatcgtc agctggcaga gtaatttgca
    15841 tggtcagcag ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt
    15901 tcgcggagat aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttgt
    15961 tggttaccac ttgcagctgc ggatcgcggc tacgttgctg atcttccaga cgcttcgcca
    16021 acaccgccag atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg
    16081 tggcgtcgga ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg
    16141 tcatatccgc tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg
    16201 tagcgttact ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg
    16261 ccgcaagccc cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact
    16321 tcttcctttc gataaacggc cataacggct cattcatcca tcttatcaca actctgataa
    16381 cgaaccttta actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca
    16441 gaatattgag taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga
    16501 aacagtaatc gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac
    16561 gccagaaggc aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac agtgtggacg
    16621 ctgtcggtgc cagccaccag cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga
    16681 ggatctgtcc cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt
    16741 tgaagtgggc gagtgcgccg aatccctgcc aggaacccat atagaatagc gacgagatga
    16801 cgtaggagat cagcaacgcc gggatcatca ccgcaaagat gatacgacgg gccagcggtg
    16861 cgccaaaaat acgcacggtc aggtcggtag caaggaaaat aaacgggaag ctaaacgcgc
    16921 cccaggtggt atggaaaccc aaaatggaga cgggaagctg aaccagatag ttactggagg
    16981 tgatcaccag cagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg
    17041 agaaaacgtt catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta
    17101 cgacacgtct gttaccttaa cataataacg tgcttaccct tttttcgagc cgccgcatga
    17161 tactgcttta cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct
    17221 tacggactca cagggcgtaa actagcgccg tttttttatg tgatgagaag aaaatgaccg
    17281 atctcttttc cagccctgac cacacactcg acgcacttgg cctgcgctgc ccggaacccg
    17341 taatgatggt gcgcaaaact gtgcgcaata tgcagcctgg cgaaacgttg ctgattatcg
    17401 ccgacgatcc ggccactacc cgcgatattc ctgggttttg tacctttatg gaacacgaac
    17461 tggttgcgaa agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgat
    17521 ggggaaagat tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagagggg
    17581 cgaggggacc gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc
    17641 ttaacgcatt cgctgtcacc agcaccgtcg cacccgtatc tgccagcacc gccagccaca
    17701 ggccggtcat ccctaacagc gtggtgacga ggaagatccc tttcagcccc agcgcaatgg
    17761 tgatgttctg gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc
    17821 cgcgcaggtg gttatgggtt aatgctgcat cagcggtttc cagcgccacg tctgtgccac
    17881 tgcccattgc aatcccaatg gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac
    17941 cgaccatcgc caggggcgca tgttgattca gcttggtcac cgctttgact ttatcttccg
    18001 gcaataggcc cgctttaaac tccagcccca gctccccggc gattgccgcc gctgcacgtg
    18061 gattatcgcc ggtgaggatc actcctttga cccccagcgc gttcagttca ctgatggcag
    18121 ttgcagcatc ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac
    18181 gtactaccag cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg
    18241 catcagcggg atgtttcccg gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt
    18301 caatgccaga cccgaccagc gcccgctgtg attcggcggt gggaatggcg agttcagcaa
    18361 cctgtgcttc gcgtacgatg gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg
    18421 ccgccagtgt cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc
    18481 gcggtttacc gacggtcagc gtaccggttt tatcaaacgc cacctgagta acacgaccta
    18541 gttgttccag cgccgctccg cctttaatca acgccccacg acgcgctgcc gccgccagcc
    18601 cggaggtaat cgccgcaggc gttgagataa ctaacgcaca cgggcagcca atcagcagca
    18661 gcgtcagccc tttataaatc cactcctgcc agctggcggc aaacagcagc ggtggcacca
    18721 gcgtcaccag cagagcgacg gccataatcg cgggcgtata gatacggctg aaacggtcga
    18781 taaaccgctc aatgggggcg cgacgctctt cggcttcttc gatcagtttc agaatccggt
    18841 caatggcgct ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg
    18901 tggctcctgc aggaacttta tcgcccgtcg cgcgctccac cggaatggat tctccggtca
    18961 gggcgctttc atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgcc
    19021 cacctgcggc gacttcaatc acatcgccag gccgcaggct gttaatcgcc acctcttccc
    19081 gctcaccgtt acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctaacgc
    19141 cctgacgcgc gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca
    19201 acaccatcgc agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca
    19261 ttaaggtttc aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa
    19321 tcgggtacag cccaaccagc gtggtcgcga taaacgccag ttgcccgaac ggatgattga
    19381 actgctccag accccagctg attgccatca tcacgattag tgtaatcagc ggcagattct
    19441 ctttcaggcg cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg
    19501 ctttttgcac cgcagattca acttgtgcac gaatgtcatt gtcggcatca accaccagtt
    19561 tttcggtggc gaacaacacc tgcacctgat tcacacctgc aagctggcgc acggcatttt
    19621 ctaccttgcg cgcacaggcg gcgcagtcca tgccactgac tttccagcta tagcgggtgc
    19681 cggagacgtt ttcagagagc gttggcgagc tggagcatgc gccgtcgcag caacagtcgt
    19741 tggcgttctg taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt
    19801 gattgtcagg tgtcgacatg gcatactccg gttaagtttt ttctcattaa ccgaaggata
    19861 cactctggag tcgactccag agtcaagttt tatcagagat acagtgagcg gatgatgagg
    19921 aagtgaccgg caaagtagca ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga
    19981 tagtggctcc ccagccagac aaagttactg ataaacagca acgaagcgcc aacaaacgca
    20041 gagagcgccg gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc
    20101 agcgtcatgc cgataaacgt gcagataggc caacgatact cttccagccg cgtccagata
    20161 atcgccagta acagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag
    20221 agcgtcatct gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg
    20281 atggcgtaca tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg
    20341 cacagccctg ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt
    20401 aacagcagga gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga
    20461 tacgatgcat ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc
    20521 ttagttcctt gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt
    20581 tagtggcgag ggtggacgga tgacaacacc ataactggca gggtatgctt atttccgcat
    20641 tttccgatga gggataaaga tgagcaaacc acctcttttc tttattgtta tcattggctt
    20701 aattgtcgtc gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga
    20761 tatggctccg ctccagcaaa agctggtggt ggtgagcaac aagcgggaaa aaccgattaa
    20821 cgatcgccgt tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctacgaggc
    20881 aagcttcaaa ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta
    20941 ccacgccctg acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgttag
    21001 ctttgttggc gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag
    21061 ttcaaacacg ccgaaaagga aaatccgtac cttttcggcg gtggtcatct gcgggccatc
    21121 tttcggtaaa gtggatttca atagcgccag ctgcatacca tgcatcagca cggtaaaaat
    21181 cagcgccacg ttaacgaaaa tatttagcgg gcgcgggaag ggatgcacca gattgaggat
    21241 taaaaatccc caaacgcaga gcattaacaa acgaccaata ttaatcagca tcactttctc
    21301 cttgtgcttc gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta
    21361 acgaccagtt tgccggaacg gtgggcagac cgttttcgac ttcgctttcg acataaatca
    21421 gggcttcgtc agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaagc
    21481 cacggcggaa cggtggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga
    21541 atgacatcgc gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct
    21601 taattaactg ctgagaaact gcacgatcca tctcaattaa cgtggccccc gcagcgtagc
    21661 gcgataacgc ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt
    21721 caacaatgac cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc
    21781 gcagacctgg gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc
    21841 ggatttggcc gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga
    21901 taacacatac ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt
    21961 ttttagctgc tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag
    22021 tcgcaaatgg cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa
    22081 gagcagaccc cggaaaaaga gacagaagtt cagaatgaac aaccggttgt agaagaaatc
    22141 gttcaggcgc aagagcctgt gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg
    22201 catactgaag ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt
    22261 gttgaaagtg aaaaagcgca gcctgaagcg gaagttgttg cacagccgga accggtcgta
    22321 gaagaaacgc cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc
    22381 aacgccgaag aggtttcgcc agaagagtgg caggctgaag cggaaaccgt agagattgtc
    22441 aaagcggcgg aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag
    22501 gcgctggcag ccgaagcagc agaagaggcg gtgatggtgg ttcctccggc agaagaagag
    22561 cagccggtgg aagaaatcgc tcaggagcag gaaaaaccaa ccaaagaagg tttcttcgcg
    22621 cgcctgaaac gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg
    22681 ttccgcggta aaaaaatcga cgatgatctg tttgaggagc tggaagagca gcttttgatc
    22741 gccgatgtgg gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc
    22801 cgcaagcagc ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggtgag
    22861 attctggcga aagtcgatga gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg
    22921 atggtgggcg tcaacggtgt gggtaaaacc acgacgattg gtaagctggc acgtcagttt
    22981 gagcagcagg gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agcagcggtt
    23041 gaacagcttc aggtctgggg tcagcgcaac aatattccgg tgattgccca gcataccggg
    23101 gcggattccg cctctgttat cttcgacgcc attcaggcag ctaaagcgcg taatatcgac
    23161 gtcctgattg ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg
    23221 aagaaaatcg tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg
    23281 actattgatg ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccacgaagct
    23341 gttggcttaa ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt
    23401 ttctcggtgg ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt
    23461 gaggatttgc gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat
    23521 taacaatgat tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc
    23581 agggcgttac gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg
    23641 cagggaaaag taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa
    23701 tctggtttag cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc
    23761 gccagattgg catgattttc caggatcacc atctactgat ggaccgtact gtctacgata
    23821 acgtggcgat cccgctgatt atcgctggtg ccagcggtga cgatattcgt cgccgggtgt
    23881 cggcggcact ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt
    23941 ccggcggtga acaacagcgt gttggcattg cccgcgcggt ggtgaataag cccgcggtac
    24001 tgctggcgga cgaaccgacc ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc
    24061 tgtttgaaga gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac gacatcaacc
    24121 tgatttcacg gcgttcccat cgcatgctca ccctgagcga tggtcacttg catggaggcg
    24181 tgggccatga ataagcgcga tgcaatcaat catattcggc agtttggtgg gcgtctggat
    24241 cgcttccgta aatcggtcgg cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg
    24301 aaatcctcgc caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat
    24361 gccttccacg gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg
    24421 atggttatcg ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt
    24481 aatcaggcgg cgacgcagta ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg
    24541 ctggacgatg acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtggag
    24601 aaagtgaact atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt
    24661 ggtggtgcgc tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg
    24721 aaactcgatt ttcaggggac ggaatcactc aatacgctgc gtgatcgtat cacgcagatt
    24781 aacggcattg acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc
    24841 gggctggtcg ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc
    24901 gtcatcggca acagcgtacg tctgagcatc tttgctcgcc gtgactccat caacgtgcag
    24961 aaactgattg gtgcgacgga tggtttcatc cttcgcccgt tcctgtatgg tggcgcactg
    25021 ctgggatttt ctggcgcatt gttgtcatta attttgtcag aaattctggt gctgcgattg
    25081 tcatcggcgg ttgcggaagt ggcacaggtt ttcggaacga agtttgatat caatggttta
    25141 tcgttcgatg aatgcctgct attgctgctg gtatgctcga tgattggctg ggtggcagcg
    25201 tggcttgcca cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata
    25261 ctctttccct gcaatgggtt ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat
    25321 ttcatctcta tgtcacattt tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga
    25381 taaaatcact gtctgataaa acagtggatg ataacctcgt tgctcttaag ctctggcaca
    25441 gttgttgcta ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa
    25501 aatgcaaagt ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa
    25561 cgcgtggccg atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca
    25621 tggcgatctg gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat
    25681 tgctcgtaat tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat
    25741 cggcctgatg aaagcagtgc gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt
    25801 cgccgttcac tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt
    25861 caaagttgcg accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca
    25921 gcgtctgggc tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac
    25981 cagcaaggac gttcgtgaga tggaatcacg tatggcggca caggacatga cctttgacct
    26041 gtcttccgac gacgattccg acagccagcc gatggcaccg gtgctctatc tgcaggataa
    26101 atcatctaac tttgccgacg gcatcgaaga tgataactgg gaagagcagg cggcaaaccg
    26161 tctgaccgac gcgatgcaag gtctggacga acgcagccag gacatcatcc gcgcgcgctg
    26221 gctggacgaa gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc
    26281 tgaacgtgta cgccagctgg aaaagaacgc gatgaaaaaa ctgcgcgctg ctatagaagc
    26341 gtaatttccg ctattaagca gagaaccctg gatgcgagtc cggggttttt gttttttggg
    26401 cctctacaat aatcaatttc ccctccggca aaacgccaat ccccacgcag attgttaata
    26461 aactgtcaaa atagctattc caatatcata aaaatcgggt atgttttagc agagtatgct
    26521 gctaaagcac gggtagccat gcataaaacg aaataaagtg ctgaacaaca acatcacaac
    26581 acacgtaata accagaagaa tggggattct caggatgaac ataaagggta aagcgttact
    26641 ggcaggatgt atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc
    26701 ggtcgtgggc gcaatgtccg gtccggttgc gcagtacggt gaccaagagt ttaccggcgc
    26761 agagcaggcg gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat
    26821 cgtaaaatat gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt
    26881 taacgacggc attaaatatg tgattggtca cctctgttct tcatcaacgc agcctgcgtc
    26941 tgacatctac gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct
    27001 gaccgcccgt ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc
    27061 gacggcggcg aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga
    27121 caaacagcaa tacggcgaag gtctggcacg agcggtgcag gacggcctga agaaaggcaa
    27181 tgctaacgtg gtgttctttg atggtatcac cgccggggaa aaagatttct ctacgctggt
    27241 ggcgcgtctg aagaaagaga atatcgactt cgtttactac ggcggttacc acccggaaat
    27301 ggggcaaatc ctgcgtcagg cacgcgcggc agggctgaaa actcagttta tggggccgga
    27361 aggtgtggct aacgtttcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt
    27421 gaccaagccg aagaactacg atcaggttcc ggcaaacaaa cccattgttg acgcgatcaa
    27481 agcgaaaaaa caggacccaa gcggcgcatt cgtttggacc acctacgccg cgctgcaatc
    27541 tttgcaggcg ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctgaaagc
    27601 gaactccgtg gataccgtaa tgggaccgct gacgtgggat gagaaaggcg atctgaaagg
    27661 ctttgagttc ggcgtatttg actggcacgc caacggcacg gcgacagatg cgaagtaatc
    27721 attaatcggc aactttgggt tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc
    27781 ggatgcggcg taaacgcctt atccggccta taagatccaa agaaatcagt aaattgcaac
    27841 acacattgta ggcctgataa gcgtagcgca tcaggcaatt tctcgttggt catcactttt
    27901 gaaatcaact tgacgattaa cgcttctccc agccgccctg ttgcgccgta aatcccagtg
    27961 cctgcataaa cgctgtcatc acaccgcgat cttccacgcc tgcatccgct atccaccagc
    28021 atgaaacgcc aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac
    28081 gtcgggtgac ttcccgcaca cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta
    28141 cccgtacggc agcgagcagg cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt
    28201 caacctgtaa agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct
    28261 ggtcgctaaa gttttctaat cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac
    28321 aaaagtggaa ccagtgtagc gaaataattt aatcgg
//
LOCUS       DAZOYS010000031        28111 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOYS010000031 DAZOYS010000000
VERSION     DAZOYS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.031"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..309
                     /gene="tnpA"
                     /locus_tag="ACHUN9_001210"
                     /pseudo
     CDS             1..309
                     /gene="tnpA"
                     /locus_tag="ACHUN9_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            369..443
                     /locus_tag="ACHUN9_001211"
                     /pseudo
     CDS             369..443
                     /locus_tag="ACHUN9_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(440..1763)
                     /locus_tag="ACHUN9_001212"
                     /pseudo
     CDS             complement(440..1763)
                     /locus_tag="ACHUN9_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1763..3379)
                     /locus_tag="ACHUN9_001213"
     CDS             complement(1763..3379)
                     /locus_tag="ACHUN9_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0694665.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3675..4391
                     /locus_tag="ACHUN9_001214"
     CDS             3675..4391
                     /locus_tag="ACHUN9_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0694666.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4388..6049)
                     /locus_tag="ACHUN9_001215"
     CDS             complement(4388..6049)
                     /locus_tag="ACHUN9_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0694667.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6245..6673)
                     /gene="ibpB"
                     /locus_tag="ACHUN9_001216"
     CDS             complement(6245..6673)
                     /gene="ibpB"
                     /locus_tag="ACHUN9_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0694668.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6785..7198)
                     /gene="ibpA"
                     /locus_tag="ACHUN9_001217"
     CDS             complement(6785..7198)
                     /gene="ibpA"
                     /locus_tag="ACHUN9_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0694669.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7192..7264)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7504..7836
                     /locus_tag="ACHUN9_001218"
     CDS             7504..7836
                     /locus_tag="ACHUN9_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0694670.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7838..9052)
                     /locus_tag="ACHUN9_001219"
     CDS             complement(7838..9052)
                     /locus_tag="ACHUN9_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0694671.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9153..10217
                     /gene="cbrA"
                     /locus_tag="ACHUN9_001220"
     CDS             9153..10217
                     /gene="cbrA"
                     /locus_tag="ACHUN9_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0694672.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10214..11551)
                     /locus_tag="ACHUN9_001221"
     CDS             complement(10214..11551)
                     /locus_tag="ACHUN9_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694673.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11626..12774)
                     /gene="dgoD"
                     /locus_tag="ACHUN9_001222"
                     /pseudo
     CDS             complement(11626..12774)
                     /gene="dgoD"
                     /locus_tag="ACHUN9_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12771..13388)
                     /gene="dgoA"
                     /locus_tag="ACHUN9_001223"
     CDS             complement(12771..13388)
                     /gene="dgoA"
                     /locus_tag="ACHUN9_001223"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0694674.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13372..14250)
                     /gene="dgoK"
                     /locus_tag="ACHUN9_001224"
     CDS             complement(13372..14250)
                     /gene="dgoK"
                     /locus_tag="ACHUN9_001224"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0694675.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14247..14936)
                     /gene="dgoR"
                     /locus_tag="ACHUN9_001225"
     CDS             complement(14247..14936)
                     /gene="dgoR"
                     /locus_tag="ACHUN9_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0694676.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15214..15870
                     /locus_tag="ACHUN9_001226"
     CDS             15214..15870
                     /locus_tag="ACHUN9_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694677.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15916..16728)
                     /gene="yidA"
                     /locus_tag="ACHUN9_001227"
     CDS             complement(15916..16728)
                     /gene="yidA"
                     /locus_tag="ACHUN9_001227"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0694678.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16843..17241)
                     /locus_tag="ACHUN9_001228"
     CDS             complement(16843..17241)
                     /locus_tag="ACHUN9_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0694679.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17481..19895)
                     /gene="gyrB"
                     /locus_tag="ACHUN9_001229"
     CDS             complement(17481..19895)
                     /gene="gyrB"
                     /locus_tag="ACHUN9_001229"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0694680.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19924..20997)
                     /gene="recF"
                     /locus_tag="ACHUN9_001230"
     CDS             complement(19924..20997)
                     /gene="recF"
                     /locus_tag="ACHUN9_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0694681.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20997..22097)
                     /gene="dnaN"
                     /locus_tag="ACHUN9_001231"
     CDS             complement(20997..22097)
                     /gene="dnaN"
                     /locus_tag="ACHUN9_001231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0694682.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22102..23505)
                     /gene="dnaA"
                     /locus_tag="ACHUN9_001232"
     CDS             complement(22102..23505)
                     /gene="dnaA"
                     /locus_tag="ACHUN9_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0694683.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23802..23882
                     /locus_tag="ACHUN9_001233"
     CDS             23802..23882
                     /locus_tag="ACHUN9_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694684.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24112..24252
                     /gene="rpmH"
                     /locus_tag="ACHUN9_001234"
     CDS             24112..24252
                     /gene="rpmH"
                     /locus_tag="ACHUN9_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0694685.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24269..24628
                     /gene="rnpA"
                     /locus_tag="ACHUN9_001235"
     CDS             24269..24628
                     /gene="rnpA"
                     /locus_tag="ACHUN9_001235"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0694686.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24592..24849
                     /gene="yidD"
                     /locus_tag="ACHUN9_001236"
     CDS             24592..24849
                     /gene="yidD"
                     /locus_tag="ACHUN9_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0694687.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24852..26498
                     /gene="yidC"
                     /locus_tag="ACHUN9_001237"
     CDS             24852..26498
                     /gene="yidC"
                     /locus_tag="ACHUN9_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0694688.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26604..27968
                     /gene="mnmE"
                     /locus_tag="ACHUN9_001238"
     CDS             26604..27968
                     /gene="mnmE"
                     /locus_tag="ACHUN9_001238"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0694689.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6779 a         7581 c         7170 g         6581 t
ORIGIN      
        1 acagaaaaac tacgcactta cttttcaaat gtatgtgctg attttgaagc tgaactggtt
       61 taaatggatg gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg
      121 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
      181 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
      241 aatagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
      301 cctggttagg tggaaaaccg cgccttatat ccccgccctg agggacgggg ttttacggcg
      361 caccggataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
      421 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
      481 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgat aacgtaatcg
      541 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
      601 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
      661 ctggtccgtt gtgcccgacc agacacggga tctcgaccat cgcttcatca tcaaagttat
      721 ggattgcccc gttgttaggc acaatcagca acatccgctc ctgagtgttg aaggcaattg
      781 ccgccgccag atcgacgatg tatgacgcat gttcgtcaat ctccagcttg gccgccgctg
      841 aatttcctgc cgctgtaatg gcgcggcaag catcgaaaac ctgtttctcg cgatgttcca
      901 ttcacctcat tcgcgcgggt atgttcaggg ttggaatgct gaacaacata atccgggaag
      961 agataatatt tcagataggt gttcggcaat gtatccgggt ccagtgcctg gacatcccgc
     1021 gcttttgcat aggtgtcgtt ccagctcgct tcagtatgtg tatcttgctg cggtggaaca
     1081 taaccgtgct tcgagacata ttgccgcagc tggggcatca ggtcgttgcc ctgcaaatct
     1141 tcaattgatg tccaccagcc aaagtggttc aggccgtagt agcgcacgcg catctgtttg
     1201 cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag
     1261 atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aatggctgcc
     1321 gggttggagt agttgagcat ccaggcattt ggtgaatatt tttccatata atccaccagt
     1381 tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcaagtttcc
     1441 tgaccaacaa caccgtggcg cagtgggatt ttttcatcca gttcgcgcat cgggtacttg
     1501 ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga acgccacttc aggatcggtg
     1561 gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca ggcttcggca
     1621 atcacttcct gccgcgcgcc atcgttatca taaaatttca gtgcacgaag cgggaaacgc
     1681 tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg
     1741 acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt
     1801 gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc agaccaatga
     1861 tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt
     1921 cgtcatccag cgtttgtgac atgtcatgca gtgcaatgcg tagacgcgtc gcacaattgt
     1981 taatgctgga gatattgccg accccgccca gggcttgcag gataccggca gcttgatcca
     2041 gctctttttt tggctctgcc gcggtggttt ggcctcgcga ggctttgtat tcggcttttg
     2101 agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaac tgcagaatca
     2161 gggtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg atttgggtca
     2221 gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa
     2281 tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc gacattgagg
     2341 ccgcaagcac agcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca
     2401 gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc aagcccgcca
     2461 cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac
     2521 caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg
     2581 gctcggcgct caaactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt
     2641 caacagctgc cggacccaag ataaacggtc cgtagatgaa gtggtgtaaa ccggttggga
     2701 tcagaatacg ttcgaggaag gtgtaaaccc agaccccaag cgcacccgcc gaacgcagga
     2761 acgcttgcag agattcaatc cccgtttgta cttttggcca gccgagcaat gtcagccagg
     2821 cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc tggaaaatgc
     2881 cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg
     2941 aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc atcattgtca
     3001 gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccaggtca
     3061 ttcccatcgc gttgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag
     3121 cacgtccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca
     3181 tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc gcgaataaac
     3241 tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac
     3301 ccaccacaat cccggcgaag ggaaacagca gcactggcgt aaacatcgcg ccgccaaagc
     3361 gttgaatttg actgagcatt tccacatcct cttttctcaa ttctgtaagt aggaagcctg
     3421 attggttatg cggcaggcct ggagcaagga taggaagatg cgatcggcag aacatgacga
     3481 cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc
     3541 gcgaaaaagt agacatctgt gagcacaggt acacagcatc gaacacatct ttaaaaaaaa
     3601 gatgtttttt caatcgatta agcagaactt gtgtgcgcat tacccgggct tgcaggcaaa
     3661 aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaact
     3721 ccgcagattt cacgctaaac agccttcttc cgggtgaaaa aaagctggcg gaagagtttg
     3781 cggtatcgcg gatgaccatc cgtaaagcca ttgacctgct ggtagcgtgg gggctggtgg
     3841 tccgccgcca cggtagtggc acttacctgg tgcgcaaaga tgtactgcat caaaccgcca
     3901 gtctgaccgg gctggtggag gtgttaaaac ggcagggaaa aactgtcacc agccaggtgc
     3961 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
     4021 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg
     4081 aagacagcta tatgccggta aaactgttcc gtaatctttc gctgcaacat ctggaagggt
     4141 cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat tatgaaagcc
     4201 tgacgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
     4261 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
     4321 tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg
     4381 aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca
     4441 ggaacatcac taacggatag acagtggcgt aagagagcgc cgccgcaccg ctagttggat
     4501 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
     4561 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
     4621 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
     4681 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
     4741 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
     4801 cgatacgccc gaggatcaac gccataatca gcagaccgcc cgccagcccc agtttcaacg
     4861 cggccgggaa tcccggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
     4921 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
     4981 tggcaacggc atcaattgcg gacggacgcc ccaccaggtt gaggatatcg ccgaactgca
     5041 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
     5101 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
     5161 tgaccaccac acgctcgacg cgcaaatcag tgtctttcgt ggacagcgaa gtatcgacct
     5221 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgacccacc agatgcagca
     5281 aatcgcccag ttggataatg gtatctggcg aaggaacttt tagggtttct tcgcgtttca
     5341 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat
     5401 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
     5461 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
     5521 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
     5581 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caaaatctgc tgccctgccc
     5641 ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc ggcagtggaa
     5701 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
     5761 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct
     5821 gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
     5881 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
     5941 gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga
     6001 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
     6061 cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt
     6121 atagcgcaca ttattacggg aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac
     6181 gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag
     6241 ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt
     6301 caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaagtt gcgccagaga
     6361 cttccatatt ttcagccagc gtaaagctca ggctaaatgg ctgattcata agcccttgat
     6421 gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac
     6481 cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt
     6541 agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctgactt tcaccggcgt
     6601 tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat
     6661 cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag
     6721 attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgccag gcggccttaa
     6781 ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat
     6841 cgatatacag caaaccattt accaggttag cgccacgaac atgaatgttc tcagctaact
     6901 ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt
     6961 gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt
     7021 cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt
     7081 caacgttata cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa
     7141 acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa
     7201 tcaatagctc ctgaaatcag cgagaatgta agaccttcca caatggacag gtcaggtagc
     7261 cagaacaccc atcaggcgcg tcctcatcga ctacgatgta aaaatgggtc tggaaatgaa
     7321 cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag
     7381 ggggcagcac gaaacgttaa agtgcgatag ccgccacaga gcgacgtaat gatgatgtta
     7441 ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt
     7501 attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatggc attactcgga
     7561 ggatgctcca gcgtgatgtc ccataccgga gggaaagaag gaacgtatcc cggcacccgc
     7621 gccagcgcca ccatgattgg cgatgatgaa accaactggg gcactaagtc gctggcgatt
     7681 ctcgatatgc cgtttaccgc tgtgatggac acgcttttat taccgtggga tgtgttccgc
     7741 aaagacagct cggtgcgctc acgcgttgaa aaaagcgaag ccaacgcgca agccaccaat
     7801 gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc
     7861 acagctggcc accttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt
     7921 ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgccg ctttgcgcat
     7981 gggcacaagc aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt
     8041 taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg ccgccctgtg
     8101 gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg
     8161 taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc
     8221 gatggtgggt aaaggttaaa cgtcgctgca cgacgccacg cggtggcgcg ggcagggtgg
     8281 tttccgttcc acttaacggc gcatcacctg ccgctttcca gccagcttca tcttgcggca
     8341 actcaacgat aaacagctcc ggcactttct caccctttgg cgaaagtgtg tcgccaataa
     8401 acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt tcatcactgc
     8461 caggctgcgg cgtgggcgtg gttttactca ccagcacaca ccagtggctg ccgctgtatt
     8521 cacgcggatg ctgcttttgt acgttgaccg gaccaaacgg cgcagcaaca ccgacgtttc
     8581 gcaaatccag cgccggatcg agttcatgca ttacatggtc gttataggta aagctcaccc
     8641 tttcaccgtt cgggctaaag acatgcacat ggctgccgcc gcgcagcgct cctggcgtat
     8701 acggcgcggt aatatccatt gcatcgagat tgctcacctt gccgccttca gcaatgactc
     8761 cgcgccgatg atggaagtcg taatgccatg tttcatcagg attttccggg ccgtgaataa
     8821 agacatattt ctctgacttc ggatgaacgg tcaccacgcc gacgtgtgcg ccctgtgacg
     8881 cacagtagat aacctcgacc tcaccggtat ggatattcac acgctcaatg gtctcgccgg
     8941 taaacgacgc cccagaagga cgcacgtcaa ataccagcca ctggctgtcg ggcgtccagg
     9001 tattggtatt ggtgagcagg tgattacggg gagcaaaggt tatctgtttc atggcgcggt
     9061 cctgatacaa aacgggtcca gccatcatac ttcacaagga cttcactttc agacaatagt
     9121 cttttgccat cgtgatgaat gtgagtgaaa agatggaaca tttcgacgtg gcgattattg
     9181 gcctcggccc ggcagggtcg gcgttggcac gaaagttagc cggcaaaatg caggtgatcg
     9241 cgctggataa aaagcaccag catggtactg aaggtttcag caaaccttgt ggcggtctgc
     9301 tggcaccgga cgcgcagcga tcttttattc gcgatgggct gacgcttcct gtcgatgtga
     9361 tcgctaatcc gcagattttc agcgtcaaaa ccgtcgacgt cgccgcatcg ctcacgcgta
     9421 actaccagcg aagctatatc aatattaatc gccatgcttt cgacttgtgg atgaaatcgc
     9481 tgatccccgc cagcgttgag gtttatcacg acagcctgtg tcgaaaaatc tggcgtgagg
     9541 atgataaatg gcatgtcatt tttcgtgcag acggctggga gcagcatatt accgcccgct
     9601 atctggtcgg tgccgatggt gccaactcga tggtgcggcg acatctctac ccggatcatc
     9661 aaatccgtaa atatgtcgca atccagcagt ggttcgcaga gaaacatccg gtgccgttct
     9721 actcctgcat ctttgataat gcgataactg actgttattc atggagtatc agcaaagacg
     9781 gttattttat ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt ttcacgactc
     9841 tgaaagagaa aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa agcgaaaaat
     9901 gcacggtgct gtttccctca cgctggcagg attttgtctg cggtaaggac aacgcctttc
     9961 tgattggtga agcggcggga tttatcagcg ccagctcgct ggaggggatt agctatgcgc
    10021 tggatagcgc agacattctg cgttcggtgt tactgaagca gccagagaag ctcaataccg
    10081 cttactggcg cgccacccgc aaactgcgtt taaaactctt cggcaagata gtaaaaagcc
    10141 gatgcctgac cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg gcacatattc
    10201 cacagttgaa ggattagcca acgcgcttca catcgcccac cagcaggata taagagagcg
    10261 cgccaatcaa cgcgacggcg gagatataaa ccagtgcagg tgcgaaaccg taaccctgcg
    10321 ccaggtaccc caccaccagc ggaacggtga tgccgcccag accaccggcg aagttaaaca
    10381 cgccgccggt taaaccaatc aggcgcatcg gtgccagaga agaaaccagc gaccaggtaa
    10441 tcgaagcaaa accgttaccg aagaatgcca gcgccatcag gcacataatc atcatcggat
    10501 cgttagtgta gttagcgccc ataatgcagg tggagatcag caagccgcag ataatcggcg
    10561 ttttacgcgc aaagcccagt gaaaagccct tacgtaccag cagatccgct acccagccag
    10621 agagcaggac gccgacaaac gccgcgagga atggcaccgt ggtcataaag cctgctttca
    10681 gcgccgtgat tcctttttcc tgggttaaat agttcgggaa ccaggttaag aaaaaccaca
    10741 gtgtagaagc caccgcaaat tgcccaagat agacgccgat cagtttacga tggaacacca
    10801 gtttccagtc tttggctgtt aacggctgac gtgcctcttt cttcactggc gcatcaccat
    10861 ccaccagacc gccgccatca cgaatgtaat ccagttcagc tttgctgatg cctttggtca
    10921 ggcgcggcgg ctgataaacc ttaaaccaaa tcagcgacca gataatgccg ataccaccag
    10981 tcacaatgaa cacccagtgc cagctcaaca tctcctgaat ccagatcagc agcggagtca
    11041 aaaacgccag accgacaaac tgaccagacg tataaaaacc aacggcggaa gcgcgttcat
    11101 gttccgggaa ccagctggtc accatccggt tattggtcgg gaaagcgggc gcttcgaaaa
    11161 taccggttat cgcacgcaga ccaattaatg acattaatcc cgtggcaaag ccctggaata
    11221 aagtcgccac tgaccagcca aatatcgcaa taaaataagt cacgcgagaa cctacgcgat
    11281 ctaaaaacca accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata
    11341 catagcccat ttccgctttc gtaatgccga actcttcctg aatatgggcg gaagccacgg
    11401 ccaggttagc gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca
    11461 gataacgccg acgccccggc tttgctgcat taacgggaat atccatagcg agctttctcc
    11521 agattttggg catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt
    11581 tgtattgctg ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac
    11641 gctgttatct tcatgacgcc agagtggatt acgccagtcc ggggcatttt tactgaactc
    11701 aataacttta gcttcgtcga tttccacgcc taagcccggt ttcgttagcg gtttaaagaa
    11761 gccgccaacc atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt
    11821 gttgtaatga attcccatac tttgttcctg aagtaccgcg ttataggaaa caaagtcgat
    11881 atgcagacaa gccgccagtg caatcggtcc gagcggacag tgcggcgcaa gggtcacgtc
    11941 ataggcttct gccattccgg caattttgta gcattcggta ataccgcctg catgggagag
    12001 atccggttgt agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga
    12061 gaacatgcgc tcacccgccg ccagtggaat atgcgtttgt gccgccaatt tcgggtagta
    12121 ttcggcttgt tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc
    12181 tttaatcagc actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc
    12241 aatctgattg ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc
    12301 gcgggagtta tcaattagcc ccagttcttc acaaccgttc agtttgaagg tatcgaagcc
    12361 gatttcgcgc agggttttaa taccgtcgat cacatccgcc ggacgatcgc cgccgaccca
    12421 actgtaggct ttaattttgt cgcgaaccag gccgcccatc agttgccaga ccgacgcatt
    12481 cagcacttta cctttgatat cccataacgc ctggtcgatc ccggcaatgg cgctcatcag
    12541 gattggaccg ccacgataaa atccggcgcg atacatcact tgccataagt cattgatgcg
    12601 ctatggatcc tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtgcg
    12661 ggcgcggcct tcaatcactg gctcgcccca gccgacgacg ccttcatcgg tttcaatttt
    12721 caggaacatc cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact
    12781 gcctctcgat acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacggactgc
    12841 ccggcgcgat agagatcgct gcctaagccc gccccagcac aacctgcgtc tatccactgc
    12901 gccaggtttt ctggcgtcac gccgccaacg gcaaagactg cgatgtccga tggcaatacc
    12961 gcttttaacg ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc
    13021 tgcgcgcccg cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc ggggcagacg
    13081 gtcatgccgt agcccaccgc acggcggatc acttcactat ggatattggg cgtaacgatg
    13141 agctgacagc ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg
    13201 ccaatcaacg ccttgtcgcc atacgcatca acgatggcgg gaatgctttg ctcccattgt
    13261 ggggaattca gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc
    13321 gccagcgcct cgtcgggctt aatgccgcgc aaaatggcga tcagcggaag tttagtttgc
    13381 cactgcatga gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgt
    13441 cacgtcgcaa cccatcgcct gaaaggcttg ctggtagcgc gcggtcaacg atgttccggc
    13501 gacaagggtg atggcgtgtt gatgggccac atagtcgcgc atactggcga cctctgcgcc
    13561 aatcaacaaa ccagagagaa attcactgac ctgttcacgg ggaagtgttc ccagcacatg
    13621 cgaggcacga acttcaaaaa gctgcggcaa tatggcgggc gtattaagac cacgctcaag
    13681 gccagccgtg aaggcatcgg cagagttttc ctgcggcggc aaacctgcac caatcaatga
    13741 gtgatttaac agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg
    13801 ctggctatcg gcctgcaccc atttgcaatg cgtaccgggc atgacataaa gagaggaagg
    13861 agccagagct cgcgcgccga tcaattgagt ttcttcgccg cgcatcacat tgtggttatc
    13921 atcatgagag acgcataatc cgggaataat ccagatattg tcgccaacag acgttaattg
    13981 ttcgccaata gacgaaaaac gggcaggaac agataaatac ggagcaactt tccagccgac
    14041 attgctgccg accattcctg ccattaccac tggcgtattc tcttcacgcc agtcggtcgt
    14101 gacttctgct aacaccgcag ccggagattt tccgttcagg cgcgtgacgc ctgcttctga
    14161 ttgcctgctc tccaggcagt ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc
    14221 ccagtcaatt gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg
    14281 gcgatcatgg taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg
    14341 aacagcgcct tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt
    14401 cgttcaaaaa ccgcccgctg cagcgaactg atcgcaatgc taagttgctg taacaccggg
    14461 ttatgcaccg actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg
    14521 cggtcctgat tgttggcaat catctcgttc agcgccgatt caatctgcgc cagatcgctg
    14581 gaggtcgcgc gctctgctgc ccaacgggca atcgccggtt ccaccagatt tcgcacttcg
    14641 ctcatggcac tgataagccg tgggtcgtag tcgttttcca gcacccattg cagtacgtca
    14701 gtgtcgaggt aattccactg gttacgcggt gccacaaacg cgccgcgata acgtttcatt
    14761 tcaatcagcc gtttcgccat caacgaacgg aacacctcac ggatgatgtt gcgcgaggtt
    14821 gcaaactcct cacagagttc cgcctcagcc ggaagcggcg agcctggtac gtatttgccg
    14881 tgaacgatct gtttacccag cgtaatgacg atgcgatcgg ttttattgag agtcatggag
    14941 agtccttgtg ctctgtatgt tcttctctac tttaccccga tcgatgcata acacggcaac
    15001 tttgtagtac cagcgtgatg acgttcgcgt ttgctgtgcg tgtaatgtag tacaaactta
    15061 tattgttgta ctacaattta gatcacaaaa agaacaatgc gtaaaaaaat gacatgcgtc
    15121 gggcagaaat ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag agtatgcttt
    15181 caacggctta gctactcgtt taaaggatta atcatgaagt tgaatcttaa gggatttttt
    15241 aagactgccg gtttattccc actggcgctg atgctttcgg gctgtatctc gtatgccctg
    15301 gtttcccata ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga caccatcacg
    15361 gggctatcgc aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg
    15421 ctggattacc ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct
    15481 cttaaccggc acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa
    15541 aagaaattta ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca
    15601 cgagtgacac aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac
    15661 ctggaaggta ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc
    15721 taccatccat ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt
    15781 gtttccgcag cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa
    15841 tttctggttg tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt
    15901 ttgcccgccc acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc
    15961 aaggttagat ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat
    16021 cgccacaccg acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc
    16081 catgatttct tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt
    16141 gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt
    16201 ctctttcact tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat
    16261 catcatcact ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag
    16321 cggaatagtg gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt
    16381 gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc
    16441 caggaaacga tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagcggc
    16501 cttctgtact agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag
    16561 ctctttcagg tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac
    16621 gccacgggcg cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg
    16681 cagcagaagg gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg
    16741 tagattggtt cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag
    16801 ggaaaagggg gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc
    16861 tttcagcagt tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc
    16921 ttgcggtgag agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt
    16981 actggtatcc acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga
    17041 ttccagttgc tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag
    17101 aatggcccct aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc
    17161 ctcaacccaa cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac
    17221 aacttcatca aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag
    17281 tatagatttg cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc
    17341 gcccttttct tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat
    17401 catgaaaatt caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca
    17461 cgctcgcatg gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca
    17521 ataaacgcac ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca
    17581 gcaatcgcat ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt
    17641 tcccacagct gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg
    17701 cgacgggact ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga
    17761 cgctcgccac gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc
    17821 gtgcagatac gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta
    17881 tccacaccgt gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg
    17941 tggacatcaa acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc
    18001 gcgttcaccc agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc
    18061 tgatagataa gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt
    18121 ttctgcgtcg cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt
    18181 gcgctggcgt tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc
    18241 atcgcttcgt cgtctttaat gtactgttcc tgcttgcctt tcttcacttt gtacagcggc
    18301 ggctgagcga tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag
    18361 gtcaacagca gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg
    18421 ctgtgataac gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg
    18481 gtgataagcg tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg
    18541 ttgaggattt tacccttcag cggcagaatc gcctggttct tgcggttacg cccttgcttc
    18601 gcagagccgc ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt
    18661 tcctggcagt ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg
    18721 gtcatttcac gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca
    18781 accacgattt tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc
    18841 tgctgttcaa ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag
    18901 aatttcgggt ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca
    18961 ccggtggcgc taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg
    19021 gtacgggtca tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg
    19081 ttgttggtaa agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg
    19141 acgccaatac cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg
    19201 ttcttgttca gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct
    19261 ttgccgtcgc gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca
    19321 cgcagacgtt tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg
    19381 ctgggccaga aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt
    19441 gcctgcggta caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc
    19501 agctccagtt tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg
    19561 gacactttat aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact
    19621 tccgccgccg atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc
    19681 tgtacagaga cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg
    19741 agcgcttcgt cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg
    19801 tcatccgtgt cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg
    19861 actttgatac tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt
    19921 gggttaatcc gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat
    19981 gtctataacg tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa
    20041 gcggctggca agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag
    20101 gtagagacac cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc
    20161 gcacatcaac agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc
    20221 gcgaatgcgt aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg
    20281 ttcaaaatta cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa
    20341 agagaaagtc agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc
    20401 gataccggcg ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc
    20461 tttatcccac gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg
    20521 cttgagcaat cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca
    20581 tccccagtcg aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc
    20641 tggcgttatc aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt
    20701 accgtcgatg cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc
    20761 gcgctcttcg ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac
    20821 gcgaccaatc tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag
    20881 cacgctggtt ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc
    20941 gagatccgcg gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac
    21001 agtctcattg gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg
    21061 ctggaaaccg aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca
    21121 tccagcacgt agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg
    21181 aggatctctt ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg
    21241 ctgacataaa gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac
    21301 gcctgcttga gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg
    21361 cggcgataat ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg
    21421 tgggcgcgaa tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc
    21481 atacgcatca gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat
    21541 tgaccaattg gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt
    21601 tcttcgcctt cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc
    21661 atagaaaact gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg
    21721 acttcgctct gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa
    21781 aaacggctac gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc
    21841 gcgccttcag gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct
    21901 cctggctcgt gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta
    21961 ccggtcagcg acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc
    22021 gtaggacgac cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt
    22081 tctacggtaa atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc
    22141 tttgatatcg tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt
    22201 cgtgtggtca cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc
    22261 tttcgccagc gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga
    22321 aaggagatcc gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat
    22381 ggtgaccagt ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat
    22441 ggtgatcgcc cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag
    22501 ctcacgtacg ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg
    22561 aatgtcattt tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg
    22621 ttcgatcgcc acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt
    22681 gatctctttc ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc
    22741 gttgaaggtg tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc
    22801 gtcgatcagc agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt
    22861 ttgcagggct ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc
    22921 attcggcttg cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag
    22981 acccgtgccg ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg
    23041 gcgagccgcc gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg
    23101 tttgacgttt acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc
    23161 tgagcgcgta gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc
    23221 gacgttgctc gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa
    23281 acgcagctgt ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag
    23341 gtacttgtcc cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt
    23401 atcgctcagt tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc
    23461 atcctgcaat cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg
    23521 aacaaaagtc gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag
    23581 accctgcttg agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct
    23641 gcaaaacgat cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca
    23701 cagattttcc cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc
    23761 cgcgaggcgg gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt
    23821 ggcttaatcg atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat
    23881 aatcctccac ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg
    23941 cgaaaaggtg cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat
    24001 tattacaatc cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg
    24061 cggatatcca ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc
    24121 acttttcaac cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct
    24181 actaaaaatg gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc
    24241 gtttctaagt aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg
    24301 cttgttaact cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc
    24361 gcaaattacc attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt
    24421 cgccaagaaa aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga
    24481 aagcttccgt ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa
    24541 aggggttgcc gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg
    24601 ccactgtcgc ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt
    24661 agtccgctac tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag
    24721 gcattgcgca ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa
    24781 tgccaccctt tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga
    24841 gaacactaac gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt
    24901 tcatgatctg gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca
    24961 cgcagacaac gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc
    25021 aggggaaact gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg
    25081 gtgatgttga gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt
    25141 tccagctgct ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc
    25201 gtgatggccc ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg
    25261 cttatgtgct ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg
    25321 caggcaacac gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca
    25381 actacaacgt gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga
    25441 agcaatccat cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca
    25501 ccttccgcgg cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata
    25561 ccattgccga taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc
    25621 aacagtattt cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg
    25681 ctaatctggg taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc
    25741 ctggtcagac tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa
    25801 tggcagctgt tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct
    25861 ctcagccgct gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct
    25921 ccattatcat catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt
    25981 acacctccat ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc
    26041 tgggcgatga caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg
    26101 ttaacccgct gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt
    26161 actacatgct gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg
    26221 acctgtcggc acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct
    26281 tcattcagaa gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct
    26341 ttatgccggt catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact
    26401 atatcgtcag caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa
    26461 aacgtggcct gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat
    26521 aagggcggtc agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg
    26581 ccacacaaag caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc
    26641 ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg
    26701 ccgaaaccgt gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag
    26761 acgccgacgg cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt
    26821 tcaccggcga agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc
    26881 tgttaaaacg cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg
    26941 aacgcgcgtt tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta
    27001 tcgacgccag ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct
    27061 ccgcacgggt caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg
    27121 caattgattt ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc
    27181 ttaatgacgt gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt
    27241 tgcgcgaagg gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt
    27301 taaacgcgct ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg
    27361 acgtgctgcg tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg
    27421 ggctacgtga agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa
    27481 ttgaacaggc cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc
    27541 cggcagagat ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg
    27601 tgcgcaataa agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg
    27661 cgttaattcg tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca
    27721 aacagagcat gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc
    27781 tacaggcgct ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg
    27841 cctgggcggg tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa
    27901 tcaccgggga atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg
    27961 gtaagtaacc gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt
    28021 tagcactcct gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata
    28081 atctttaaaa aaagcattta atattgctcc c
//
LOCUS       DAZOYS010000032        27872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOYS010000032 DAZOYS010000000
VERSION     DAZOYS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.032"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            82..744
                     /gene="acrS"
                     /locus_tag="ACHUN9_001239"
     CDS             82..744
                     /gene="acrS"
                     /locus_tag="ACHUN9_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0694690.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(747..926)
                     /locus_tag="ACHUN9_001240"
     CDS             complement(747..926)
                     /locus_tag="ACHUN9_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0694691.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHUN9_001241"
     CDS             complement(1010..1894)
                     /gene="yhdJ"
                     /locus_tag="ACHUN9_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0694692.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHUN9_001242"
     CDS             complement(1980..2276)
                     /gene="fis"
                     /locus_tag="ACHUN9_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0694693.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHUN9_001243"
     CDS             complement(2302..3267)
                     /gene="dusB"
                     /locus_tag="ACHUN9_001243"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0694694.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHUN9_001244"
     CDS             complement(3596..4477)
                     /gene="prmA"
                     /locus_tag="ACHUN9_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0694695.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHUN9_001245"
     CDS             complement(4489..5940)
                     /gene="panF"
                     /locus_tag="ACHUN9_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0694696.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5930..6172)
                     /locus_tag="ACHUN9_001246"
     CDS             complement(5930..6172)
                     /locus_tag="ACHUN9_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0694697.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHUN9_001247"
     CDS             complement(6281..7630)
                     /gene="accC"
                     /locus_tag="ACHUN9_001247"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0694698.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHUN9_001248"
     CDS             complement(7641..8111)
                     /gene="accB"
                     /locus_tag="ACHUN9_001248"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0694699.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9089..10063)
                     /locus_tag="ACHUN9_001249"
     CDS             complement(9089..10063)
                     /locus_tag="ACHUN9_001249"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0694700.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10215..12155
                     /gene="csrD"
                     /locus_tag="ACHUN9_001250"
     CDS             10215..12155
                     /gene="csrD"
                     /locus_tag="ACHUN9_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0694701.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12460..13503
                     /gene="mreB"
                     /locus_tag="ACHUN9_001251"
     CDS             12460..13503
                     /gene="mreB"
                     /locus_tag="ACHUN9_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0694702.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13569..14672
                     /gene="mreC"
                     /locus_tag="ACHUN9_001252"
     CDS             13569..14672
                     /gene="mreC"
                     /locus_tag="ACHUN9_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0694703.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14672..15160
                     /gene="mreD"
                     /locus_tag="ACHUN9_001253"
     CDS             14672..15160
                     /gene="mreD"
                     /locus_tag="ACHUN9_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0694704.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHUN9_001254"
     CDS             15169..15762
                     /gene="yhdE"
                     /locus_tag="ACHUN9_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0694705.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15752..17221
                     /gene="rng"
                     /locus_tag="ACHUN9_001255"
     CDS             15752..17221
                     /gene="rng"
                     /locus_tag="ACHUN9_001255"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0694706.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHUN9_001256"
     CDS             17289..21089
                     /gene="yhdP"
                     /locus_tag="ACHUN9_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0694707.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNAGLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21245..22690
                     /gene="tldD"
                     /locus_tag="ACHUN9_001257"
     CDS             21245..22690
                     /gene="tldD"
                     /locus_tag="ACHUN9_001257"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0694708.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22818..23747)
                     /gene="aaeR"
                     /locus_tag="ACHUN9_001258"
     CDS             complement(22818..23747)
                     /gene="aaeR"
                     /locus_tag="ACHUN9_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR0694709.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23930..24133
                     /gene="aaeX"
                     /locus_tag="ACHUN9_001259"
     CDS             23930..24133
                     /gene="aaeX"
                     /locus_tag="ACHUN9_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0694710.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24141..25073
                     /gene="aaeA"
                     /locus_tag="ACHUN9_001260"
     CDS             24141..25073
                     /gene="aaeA"
                     /locus_tag="ACHUN9_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0694711.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMDHRLREFG"
     gene            25079..27046
                     /gene="aaeB"
                     /locus_tag="ACHUN9_001261"
     CDS             25079..27046
                     /gene="aaeB"
                     /locus_tag="ACHUN9_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0694712.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27138..27410
                     /locus_tag="ACHUN9_001262"
     CDS             27138..27410
                     /locus_tag="ACHUN9_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0694713.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27466..27729)
                     /gene="yhcN"
                     /locus_tag="ACHUN9_001263"
     CDS             complement(27466..27729)
                     /gene="yhcN"
                     /locus_tag="ACHUN9_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0694714.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6704 a         6971 c         7394 g         6803 t
ORIGIN      
        1 taaatacaca ttagatgata gattaacctt cgctattttc tcaccctgtg tcgaatatat
       61 ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg
      121 caagaactga ttgaaactgc catcgcccag tttgcgcaac atggcgtaag caagacgacg
      181 ttcaacgaca ttgccgatgc cgctaacgtt acgcgtggcg ctatctactg gcacttcgag
      241 aacaagaccc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc
      301 caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg
      361 attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta
      421 tatcacaaat gtgaatttaa tgatgggatg ctggccgagg gagtgatacg cgaaaagatg
      481 ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta
      541 gcaaataacc tcgatttaga tgttgtgatg attattattg atggtgcctt cagcggaatt
      601 gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc acccgcgctg
      661 gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa
      721 acgaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag
      781 ttaaccagtt ttagtggata aactgagttc atattacggc atttgtctgt ttctgttgtc
      841 agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg
      901 acgagccacc aatacttgcg aatcattacc tgagcctgtg catgtttaac caagtctggc
      961 aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa
     1021 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
     1081 ttttcacttt cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc
     1141 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg
     1201 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
     1261 aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat
     1321 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
     1381 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
     1441 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
     1501 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
     1561 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
     1621 gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc
     1681 actctacaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
     1741 gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagatcaga tcgacgcttt
     1801 cagtaggtag ctttttaagt tcggcaaggg catcaccgtg aataatggtc ttagcttcat
     1861 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
     1921 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
     1981 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     2041 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     2101 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     2161 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     2221 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     2281 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     2341 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     2401 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     2461 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     2521 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     2581 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     2641 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     2701 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     2761 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     2821 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     2881 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     2941 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     3001 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     3061 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     3121 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     3181 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     3241 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     3301 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     3361 gatggatcaa agaattgcat ttaaaatgag cgtggtgaga taattactca taaaaatcat
     3421 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     3481 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataaccggca aatttacgtg
     3541 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat
     3601 tcttacgacc ggtaatgcgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     3661 tgtcgacata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     3721 gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     3781 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     3841 agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggcta gcctgaatcg
     3901 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatag
     3961 ccagaatgcc ggaaccacag ccaaagtcga tgactatttt accggttaaa tcgaggctgt
     4021 cgagccactg caggcacaga gaggtagttg gatgggtacc cgtaccaaac gccagccctg
     4081 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     4141 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     4201 tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     4261 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     4321 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     4381 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tcagcgttcg
     4441 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     4501 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     4561 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     4621 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     4681 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     4741 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     4801 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     4861 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag
     4921 caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     4981 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     5041 atcggggatc actgcacgac ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc
     5101 gactacaatc gtaccgatga tgatcccccg gtgtaccgct ttgctgtctt tataagagat
     5161 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
     5221 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
     5281 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
     5341 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
     5401 ggcgcgaaag ccgccaaagg cggtatacaa cgcaatgctg ataccgaaaa tcagcagccc
     5461 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
     5521 ggtcattgcc ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
     5581 actctggtag cgggcaaaca gcatgtcgtt cagcgtcact gcattgtagc ggcgcgcaag
     5641 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat
     5701 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
     5761 actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
     5821 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
     5881 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
     5941 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
     6001 taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
     6061 cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca
     6121 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
     6181 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
     6241 aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga
     6301 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
     6361 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
     6421 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
     6481 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
     6541 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
     6601 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
     6661 tgatcgacag cggttgaccg gcagcgatac ggagctgttc tttgatcagg tcaacgccag
     6721 tgatcatttc ggtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
     6781 actcgccgtt ttcgaacaga aactcgaaag tacctgcacc gcgatagccg atatcaacac
     6841 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
     6901 gcgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
     6961 agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
     7021 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
     7081 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
     7141 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
     7201 tatccatatc gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt
     7261 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
     7321 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
     7381 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
     7441 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
     7501 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
     7561 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
     7621 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
     7681 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
     7741 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
     7801 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
     7861 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
     7921 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
     7981 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
     8041 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
     8101 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
     8161 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
     8221 gggacgccgg tctattttgc cccagagtat caatgttaga cttgacggac attgtgcagc
     8281 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
     8341 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
     8401 caattggcag ctaaatactg gtctcatcag ggaagataat caacagctaa catgtaaata
     8461 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
     8521 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
     8581 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
     8641 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
     8701 tagatgtgaa tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg
     8761 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat
     8821 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
     8881 aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
     8941 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
     9001 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
     9061 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
     9121 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
     9181 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
     9241 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
     9301 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
     9361 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
     9421 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
     9481 tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
     9541 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
     9601 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
     9661 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
     9721 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
     9781 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
     9841 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
     9901 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
     9961 gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg
    10021 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    10081 atgatggatt ggaaaataat ttctgaagat tatactcgct aatggaaaag caaagccgat
    10141 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    10201 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    10261 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt
    10321 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    10381 atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    10441 gtgtagacct gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc
    10501 gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg
    10561 ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga
    10621 ctaccgcgcc gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac
    10681 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    10741 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    10801 gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    10861 ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac
    10921 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    10981 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca
    11041 gccaggttga agaacagttc ttcactctga ctaatctgct gtcgacattt atgatgcgct
    11101 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    11161 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    11221 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    11281 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    11341 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    11401 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    11461 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    11521 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    11581 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    11641 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    11701 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    11761 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    11821 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    11881 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    11941 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    12001 ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc
    12061 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    12121 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    12181 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    12241 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    12301 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    12361 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    12421 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    12481 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    12541 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    12601 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    12661 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    12721 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    12781 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    12841 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    12901 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    12961 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    13021 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    13081 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    13141 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    13201 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    13261 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    13321 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    13381 atggaagaaa ccggcattcc agtagttgtt gctgaagacc cgctgacctg tgtggcgcgc
    13441 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    13501 taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg
    13561 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    13621 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    13681 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    13741 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    13801 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    13861 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    13921 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    13981 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    14041 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    14101 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    14161 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    14221 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    14281 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    14341 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    14401 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    14461 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    14521 tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc
    14581 ccacacagcc tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    14641 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg
    14701 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    14761 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    14821 gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    14881 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    14941 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    15001 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    15061 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    15121 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    15181 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg tgtgaccttt
    15241 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    15301 gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc
    15361 ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc
    15421 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    15481 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    15541 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    15601 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    15661 ggcagctatc acgccgtagt cggcttacct ctggttgaaa cgtatgaatt attaagtaat
    15721 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    15781 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    15841 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    15901 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    15961 atccgatatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    16021 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    16081 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    16141 ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga
    16201 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    16261 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    16321 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    16381 cggcgaactg gcgctggctc agcgtgttct gcgtgatttc gccgacgccg aactggaccg
    16441 cattcgcgtt gactcacgcc tgacttacga agcattactt gagttcacct cggagtacat
    16501 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    16561 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg
    16621 ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    16681 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    16741 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    16801 tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa
    16861 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    16921 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    16981 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    17041 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    17101 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    17161 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    17221 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    17281 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    17341 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    17401 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    17461 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    17521 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    17581 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    17641 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    17701 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc
    17761 catccggtca gcgtgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    17821 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    17881 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    17941 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    18001 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    18061 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    18121 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    18181 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    18241 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    18301 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    18361 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    18421 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    18481 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    18541 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    18601 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    18661 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    18721 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    18781 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcactatttc ccggaaaact
    18841 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    18901 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    18961 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    19021 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    19081 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    19141 cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    19201 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    19261 acggcgatgt gaatgctggc ttacatcttg atatcccgct gaacggcgag ctggtaaccg
    19321 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    19381 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    19441 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    19501 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    19561 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    19621 atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg
    19681 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    19741 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    19801 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    19861 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc
    19921 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    19981 gtgcagcgag tttcccacaa tacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    20041 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    20101 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    20161 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    20221 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    20281 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    20341 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    20401 ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac
    20461 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    20521 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg
    20581 aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    20641 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    20701 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    20761 ccgcgtggat taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg
    20821 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    20881 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    20941 ttgttggcgc ggcagttttt gccgctagta aagtgctggg gccgctgtgg agcaaagtct
    21001 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    21061 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    21121 atatgataat cgttgccaaa ggccaacgag ccagaacata accgtaggcc ggatagggcg
    21181 ttcatgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    21241 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacagcc tgaaacatca
    21301 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    21361 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    21421 ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    21481 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    21541 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    21601 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    21661 tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    21721 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    21781 ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    21841 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    21901 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    21961 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    22021 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc
    22081 agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    22141 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    22201 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    22261 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    22321 catgccgcgt atgactaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    22381 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    22441 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    22501 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    22561 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    22621 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    22681 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    22741 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    22801 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    22861 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    22921 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    22981 aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    23041 atcccggcac ccgccgtcag ccagcgcacc agcgtcatcg gatcattagt cacaaatctt
    23101 ccttgtggga tcaggcgagt cgagatccct tccggtgcga tcagttcaaa ttcattgtcg
    23161 ggccgcacgc tgtattcaag ccatgaatga ctactcaaat cggcgggttt ttccggtatg
    23221 ccgtattgtg tgagatagct tttcgcggcg cacaccacca tcggcatcgc gcccagacgg
    23281 cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg
    23341 gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct
    23401 ttcagcattt ttgctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca
    23461 atgcgcagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc
    23521 acttcatgaa gcatccgacg gcagccctgg tagtaaattc tgccggcttc ggtcaggcca
    23581 atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc
    23641 gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca
    23701 aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac
    23761 ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc taatatatta
    23821 aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac
    23881 agatgctgaa aataataacg cctgctctct ctttacaacc aaggtcaaca tgagtctgtt
    23941 tcccgttatc gtggtgtttg gactgtcctt cccaccgata ttttttgaat tgcttttatc
    24001 actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt
    24061 ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg
    24121 actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca
    24181 cggtcgtatt agtcattcta gccttcatcg caatttttaa tgcctgggtc tattacaccg
    24241 aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg
    24301 tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga
    24361 tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg
    24421 ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc
    24481 tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg
    24541 ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac
    24601 gcacggtgat ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg
    24661 agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac
    24721 tggcctatat ggaagaaact aagctggaag gggggcgtcc ggggtatcgt gcagagatca
    24781 cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gcaggggtca
    24841 ccaacgccag cagcacgcgt gacgacaaag ggatggcgac tatagactct aatcttgaat
    24901 gggtgcgtct tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca
    24961 tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa
    25021 gccaggattc gttcttccgt aaaatggacc atcgcctgcg tgagtttggt taatcacgat
    25081 gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat
    25141 tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact
    25201 gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg
    25261 cgctattcgc tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg
    25321 actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat
    25381 ctgggccggt ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg
    25441 ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct
    25501 tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat
    25561 tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc gagagctgga
    25621 aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga
    25681 agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcactac aaggcatgcg
    25741 cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat
    25801 caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac
    25861 gcgcccggaa ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc
    25921 gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg
    25981 ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa
    26041 gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga
    26101 accggaagta aaagtagagt cagccgaacg tcatcatgca atggttaact tctggcgaac
    26161 cacactttcc tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag
    26221 tggtgcaatg gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg
    26281 catggtggcg atcgacttta tctatgggac gctggccgcg ctgccgttag ggctgctcta
    26341 ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc
    26401 agtgctggga ttcttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc
    26461 actggccagc accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca
    26521 gtttctcgac agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt
    26581 gctggtgcgg gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttt
    26641 tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc
    26701 actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg
    26761 cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa
    26821 cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc
    26881 ccgtagcgat gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta
    26941 ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct
    27001 ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc
    27061 caaaagcgtc ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa
    27121 cgtgacggtg gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg
    27181 aggattttta tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc
    27241 tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat
    27301 ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg
    27361 aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa
    27421 agcccccgaa ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc
    27481 agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc
    27541 tttctcttcc gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc
    27601 acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagtat caatggagtc
    27661 ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt
    27721 gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat
    27781 atcacaaaat caagcataca ctaattattg aactaattaa taccacgcta actatttcag
    27841 ttgatactaa gtgttaaaaa tgtgaatttt aa
//
LOCUS       DAZOYS010000033        27152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOYS010000033 DAZOYS010000000
VERSION     DAZOYS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.033"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..415
                     /locus_tag="ACHUN9_001264"
     CDS             <1..415
                     /locus_tag="ACHUN9_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0694715.1"
                     /translation="LRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMA
                     SLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGD
                     AVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(463..1335)
                     /locus_tag="ACHUN9_001265"
     CDS             complement(463..1335)
                     /locus_tag="ACHUN9_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0694716.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(1347..2441)
                     /locus_tag="ACHUN9_001266"
     CDS             complement(1347..2441)
                     /locus_tag="ACHUN9_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0694717.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2474..3472)
                     /locus_tag="ACHUN9_001267"
     CDS             complement(2474..3472)
                     /locus_tag="ACHUN9_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0694718.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3497..5008)
                     /locus_tag="ACHUN9_001268"
     CDS             complement(3497..5008)
                     /locus_tag="ACHUN9_001268"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0694719.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(5031..6014)
                     /locus_tag="ACHUN9_001269"
     CDS             complement(5031..6014)
                     /locus_tag="ACHUN9_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0694720.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(6111..9388)
                     /locus_tag="ACHUN9_001270"
                     /pseudo
     CDS             complement(6111..9388)
                     /locus_tag="ACHUN9_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            9506..10699
                     /locus_tag="ACHUN9_001271"
                     /pseudo
     CDS             9506..10699
                     /locus_tag="ACHUN9_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10897..12150)
                     /gene="glyA"
                     /locus_tag="ACHUN9_001272"
     CDS             complement(10897..12150)
                     /gene="glyA"
                     /locus_tag="ACHUN9_001272"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0694721.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            12478..13668
                     /gene="hmpA"
                     /locus_tag="ACHUN9_001273"
     CDS             12478..13668
                     /gene="hmpA"
                     /locus_tag="ACHUN9_001273"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0694722.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13713..14051)
                     /gene="glnB"
                     /locus_tag="ACHUN9_001274"
     CDS             complement(13713..14051)
                     /gene="glnB"
                     /locus_tag="ACHUN9_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0694723.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14112..15446)
                     /gene="glrR"
                     /locus_tag="ACHUN9_001275"
     CDS             complement(14112..15446)
                     /gene="glrR"
                     /locus_tag="ACHUN9_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0694724.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(15436..16149)
                     /gene="qseG"
                     /locus_tag="ACHUN9_001276"
     CDS             complement(15436..16149)
                     /gene="qseG"
                     /locus_tag="ACHUN9_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0694725.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(16314..17741)
                     /gene="qseE"
                     /locus_tag="ACHUN9_001277"
     CDS             complement(16314..17741)
                     /gene="qseE"
                     /locus_tag="ACHUN9_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0694726.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(18299..22186)
                     /gene="purL"
                     /locus_tag="ACHUN9_001278"
                     /gene_synonym="purI"
     CDS             complement(18299..22186)
                     /gene="purL"
                     /locus_tag="ACHUN9_001278"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0694727.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            22444..24000
                     /gene="mltF"
                     /locus_tag="ACHUN9_001279"
     CDS             22444..24000
                     /gene="mltF"
                     /locus_tag="ACHUN9_001279"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0694728.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(23997..24500)
                     /gene="tadA"
                     /locus_tag="ACHUN9_001280"
     CDS             complement(23997..24500)
                     /gene="tadA"
                     /locus_tag="ACHUN9_001280"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0694729.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(24558..25193)
                     /gene="yfhb"
                     /locus_tag="ACHUN9_001281"
     CDS             complement(24558..25193)
                     /gene="yfhb"
                     /locus_tag="ACHUN9_001281"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0694730.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            25402..26250
                     /locus_tag="ACHUN9_001282"
     CDS             25402..26250
                     /locus_tag="ACHUN9_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0694731.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(26589..27146)
                     /gene="pinE"
                     /locus_tag="ACHUN9_001283"
                     /pseudo
     CDS             complement(26589..27146)
                     /gene="pinE"
                     /locus_tag="ACHUN9_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         6481 a         7471 c         6838 g         6362 t
ORIGIN      
        1 actacgttat tttgattttg gagctgcccg ccccgttttg ttattaattg cccgtatcgc
       61 cgtggtctta attttcatta tttttggttt tcccaaaatg atgggctttg acggtacggt
      121 ccaatatatg gcctcgttgg gcgcgccaat gccgatgctg gcagcgatta ttgcggtagt
      181 tatggaagtg cccgccgcga tattaatcgt gcttggcttt ttcacccgtc cgctggcggt
      241 gctgtttatt ttctacacgc tgggtacggc ggtgattggt caccattact gggatatgac
      301 cggcgatgcg gttgggccaa atatgattaa tttctggaag aatgtcagta tcgctggcgc
      361 gttcttgcta ttggcaatta ccgggccggg ggcaatttct ctcgatcggc gttaggaaag
      421 atgccggatg cggcgtgaac gccttatccg gcatttaata aattacagcc actctacccg
      481 caacgacatc tctgaagttg ttgattcccc tggtgctagc gcaatgagat cacccccttc
      541 cgggcgatga tgatcatccg gcgcatggct catcggttcc agacagaaga aatcaaacgc
      601 atatccttta tcaaacgcag ggtcggaaac aaagatgaaa taacacggtg caggtggcgt
      661 cgtttccatg atgatggcat aaccttcctg cggctgctcg atgcgggcct gaccattcca
      721 tccggcaaaa ccattgttca cccactggcg cggcaacggc gcgagctggt taaaatccag
      781 ttcctgcggt agctgctcgc aaaactcacc cgccagccac tgctcccgtt ccagccagta
      841 accgctcgcc tgcgcctgaa tccgcgtttg tggtgacaac ggaaaataag gatgccagcc
      901 ggtaccaaat ggcagcgtct ctgccccttg attggtgaca gagagcgtca ccgtcagcgt
      961 atccgccgtt aaatgaaacg cctgacttac ccgatagtga tagacaccgc tgcgatgttc
     1021 atacaccaga cacaaactat catcgctgtg cgagacacat tgccactcgc ccagccagcc
     1081 atcgccgtgt agatagtgcg catcccactc aacattcggt tgcagttgat actcacgccc
     1141 ctgccagaca aaacggttgc cgctcacccg gtttgcaaat ggcaccagcg gaaaacatga
     1201 ggcatcggtt gccacaccgc tttttttacc aggacgtaag agcggcgtcg tatcgcgcca
     1261 gaagccttcg attacgccgc cctggtcaga aacgtccagc tttagcgacc cgtgggataa
     1321 ggtatagatg gtcatccgct aactccttaa tccgggaagt taatcacaac tttcccgcat
     1381 ttgccgctcg ccatcagcgc ataggcatca cctgcctgtt ccagcgagaa gcgatgggta
     1441 atggcgttac gcggccacag cttccagtca gtcaaatcgt gggcgcattt ttccatatgg
     1501 aacagactgg tcacccagga gccgataatc cgccgttgat ggtgcatcag atcggcgctg
     1561 acctcgaatt ccacttttcc ggtttcacca atgtaaacca cccgtcccca gtcagcggtg
     1621 gattgcagtg ccagcaagcg acctgcggca ttaccagaac aatcgagcgc aacatccgcg
     1681 ccaccgtggg tgagttcggc gataatctgc ggcagacctt cggtggttgc taaatagccg
     1741 tgatccatca cccctaactg ttttgccatc gccagacgtt ccggcagcat atcaacgccg
     1801 atgatccgtt ttgcaccgcg acctttcgcc agcatcatcg ccatcatgcc gaccggcccc
     1861 agaccgacca ccagcacgtt atcgctgccg gaaacttcgc cgcgcaaaat cccttcatag
     1921 gccgtgccaa cgccgcaact gataaacgca ccatcttcgt agctcagcgc atccggcagg
     1981 aggatcagat ctttttcttc cgccagcaga tattcggcat gaccgccgtc acgctgccag
     2041 ccgtaagccg cttttccttt gccagtacaa gagataggaa agccgcggcg gcagttcggg
     2101 caaaaaccac agccagaaat gtgatacacc agcacgcggt cgccctcttt aaaatggcgg
     2161 cagccttgcc ccatcgccac aatctgcccg cacggttcat gaccgttgat aaatccctgg
     2221 tataacggtt tatcgggtgc cgccgccgtg gcacagtgtt gatgatagat atagtggaca
     2281 tcgcttccgc aaatcccgga ggatttcatt ttgatcagta cctggttaat ccccggcgtc
     2341 ggcaccgcaa cttcccgcag atcgacggtt gaatttcctg gtaaataagc tgccagcatc
     2401 gttttcataa atccctcatt aacaatacga ttaattttca tccctgccgc acccgcgcca
     2461 ggggcgttaa ggtttagcgt ttcgctttac tgcttcgctg ggtcagcaag atattcgcca
     2521 gcaccgccac cacgatgatg acgccgcgta ccacctgctg gaaaaaggag ttaataccga
     2581 gcagcaccag accgttaccg attagcgtaa tcaccagcac accaagcaat gtaccgaaca
     2641 gggaaccgcg accgccggaa agtgccgtac cgccgaccac gaccgcggcg atgacgtcaa
     2701 actccagacc gtttgcggca cctgcgttac cggaaccgag gcgtgccgcc agcaaaatgc
     2761 cggtcaccgc cgctaataat cccgaaaggg taaagataag aatgcgcacc cgacgaacgt
     2821 taataccgca caactgcgcc gccgtggcat taccgccaac ggcaaaaacc gagcgcccga
     2881 aggcggtttt tcggctaatg aacacaaaca gcgcaaacaa caccatcatg atcagcgcgg
     2941 atactggcac accgagaaat tgtccgccca gccagtccag cacctcgttt tcgtcaatcg
     3001 gcactggcag tgcgttggtc ataaacagcc ccattccgcg cagggcgctc cacagcccca
     3061 gtgtggcaac gaaacttggt acgttaaaca cgccgcgcag caccccggcg agcgttccca
     3121 tcagcgcgcc taacagcaac accagcagac aagcaatcgc caacggaact tcaaattgca
     3181 gcaaaaatgc caggcacacc gagacaaaag ccaccatcgg cccaacgctg acatcaattt
     3241 caccggagat aataatcagc gtcatcgccc aggcggcaat cccaatggtg gcggcatcgc
     3301 gcagcacgtt catctggtta ttcaatgaga taaagccagg cgcgttcagg gagaagacca
     3361 gataaagaat ggcaatcacc accagcaaac cgatctcatt aatatggcga ctgacaaatt
     3421 gtttgagcga gacgctcttg ccctgcggca atggtaatga cgaagcagac atcttcaggt
     3481 tcctcggtaa aattcatcag tgcacagaca gaatggcgga catcagctca tccacattga
     3541 ccggcgagtg aaactcctgc gagaacgtgc cgtgctgtaa taacaggatg cggtcacaca
     3601 ccagcggcaa ctcctccact tcactggaga taaacaccac gctttttcct tcggcagcca
     3661 gctcacggac aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgttggct
     3721 cgtcgagcaa caaaatctgg ctggcggcat agacccaacg accgatcacc actttttgct
     3781 gattgccacc agaaagcgtg ccgatgggcg tttcgctact agcggccttg accgtcatcc
     3841 gctgcatcac ctcttcggtc aggcggcgga tggtggacca ttgcagcact ccgttggcgc
     3901 tgattttttg ccgattggtc agcactgtat tttcgtcaac gcccaaccag ggaatgatcc
     3961 ccgcttcttt gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat
     4021 cggggcgcgt gattttctcg ccgttgataa caatttcgcc ctgttcatac gtctccagcc
     4081 caacaatcgc cttcagcaat tcactgcgcc ctgcccccag caggccagca atgccaagca
     4141 cttcgccgcg acgtaacgta aagctgatat cctccagctt gggcttatgg cgtaacgcac
     4201 ggacttccag tacggcctga tccacaattt cctgaggggc aaccggcgca atatcaacgt
     4261 gatcacgccc tagcattagc gacacaatat gatgcgtgga ggtgttttcg agcatcacat
     4321 cgcccgccac ctgaccatcg cgcataacgg tggcacagga ggcaatgcgg cggatttctt
     4381 ccatccggtg gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcaccgcac
     4441 tgatcaccag ttcaacttct gcactggcaa gcgagctggt gggttcatca agaatgacca
     4501 cgcgcggctc gcccttcatc acccgtgcaa tttccaccag ttgcttttgc gccgggctta
     4561 gcgttgaaac aagttgttca ggactaacgt caacgcccag cgcctgtaag caacgttggg
     4621 catcctgcgc catttgcagg taatcaatca tgccgttgcg gcgaggccat tgaccaaggc
     4681 agaggttttc tgccaccgtc agcccttcca ccagacttaa ttcctgataa accgcacgaa
     4741 cccccagttc agcggcacgg cgagtcaacg tagcttcgtc accttccagt cgcgtctcgc
     4801 caatccagat atcaccgcta tccgggcgtt cgctgccggt aagcattcga atgagagtcg
     4861 atttgcccgc gccgttttta cctaacagcg cacgaacttc gcctttattg agcgtgaagt
     4921 taacgttatc cagcgcaacg acgccggggt aacgcttatt tcctgccacc acttttgcta
     4981 ccgggactgc ctctgttgcc gtgaacatag ggacctctgc gaatcagcga ttagggcaga
     5041 ccatcaacgt gcgttgccag ccactgttta ccatcttccg ttttggtata gagatcgatt
     5101 ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg
     5161 aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg
     5221 ttttccagct cctgagcaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg
     5281 gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgactccccc
     5341 ataatggcgt tgagatccgg cgtggagata atcagttttt caccaacgga aatcgcttta
     5401 tttaaaacag tcccttcctg attagcgaca atttgcgcgc cgggaacgcg ggattttaat
     5461 acttcttcaa atcctttacg tcgctgcaca caaacttcaa aggcttcgca attgatgacg
     5521 gcaattttcg gctggtcaat tttattggca ataaaataat cggcggcagc gttacccagt
     5581 tttttaccaa attccagtgg atcgccgacc agatacgccg agacatattt atcgacaccc
     5641 ttttgattaa tacaggtgtt gtagcaaatc accggaatac ccgcttcact ggcgcgacga
     5701 acggtacggc tactgccatt ttcagacact gccgataaaa taatggcatc gacattacgc
     5761 gccacgaggg tatcaacaaa ggtactttct ttcgaaatat caccctgggc gttagtttca
     5821 attaactgta cctgtactga agaatctttt gccgcatcct gaacgccctg acgcactcca
     5881 gcgtaatatc ccagggtatc aaggtatatt gcaccaatgg tcatttcttt atccgcagcc
     5941 ctggcaaata gtgcgctacc taatagcgta gccattaata atagattacg ggttgttctc
     6001 atttttttag gcatagtgcc tcctgtagag tttttattaa caacggctta ttctaattat
     6061 tttgtgatga gcggcagcgc gtgccgctca aatattacag ttaatagggt ttagtgaacg
     6121 tagttaaaaa taaaaggcat cacggtggtg aataatgccg ctttcggcca ggccggatta
     6181 atcgccgtca attcagcgcg agcagattca tattgcgcaa cctccccgag tcccgcggac
     6241 gccagcatcg ccaccatcag gcatttttct ttatgctgct gttgtaaatc gccatacagc
     6301 gacaacaggt caggttgtga aacagcaaag aaatccgcct caatactggt tttcgccatc
     6361 tcttgcgccc actgtttcat ttcactaaac agttgctgtg cgatttgttg ttcgcccagc
     6421 agtcgcagcg ccattccttg ccagaagaga taatcaaccg gctgatcgtt gtaataactg
     6481 tggatgttaa tggtgcgatc gccggtcgcc gccagacgta aacaacgcgt cgcttcagtt
     6541 tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc agatgtcgtt atcagtttgc
     6601 cccggtaaac ggccttcgct taaattctcc ggataatgca gcgcggcatg aagcagttcg
     6661 caggcctgct gcggctgtct ggcatcaaga tgctgccagg cgcgtaataa ctggttgagg
     6721 ataaactgac tggtgacctt cccttccccg ccttcccacg ggtggaattt gcgcgtggcg
     6781 agaatgtccg ccgctttgtc tgcctgaccc gtgagatgcc acaaattgag cagttctgcg
     6841 gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt ccagacgcgc cagtcgtttc
     6901 tccggtgtgg ctccacttaa cttatccagc aaatcccgtt cgaaaagcag acgtgcatcc
     6961 tgcggcgcaa gctgataagc attatcaaga taacgcgcgg ccagttcata atcgtgttgc
     7021 ttattccacg catggatcgc caacccgcgc cagccatcgg caaactccgg cgacatctct
     7081 acgcaacgtt gccataaagt aatggctttg ttgtagctgc gcttgttgta gtagaagcag
     7141 gccagtaaat gacgggcaaa ccagcactct tcaatactct ccagcgccgc cacttcttcc
     7201 agcgtattcg ggaaacggac aaactgcggg aagacatcaa tggcttttgc gaccagttcg
     7261 ccgcgttcgg ctttcggcag caagctggct tgtagataaa gcgataacgt gcgctgacag
     7321 tccagtgcgt tcagcatctc tgtcgccaga gtgggcattc cccagttaat cagttgcccg
     7381 gcggtcatca gggcgttaac gtcgcgtccc tgacacagac cgcgccactg cgcgagggct
     7441 gattcgctac gaccatcgaa ccagttcagc caccacagaa tggcgttcag cggataatca
     7501 cgcagcagtt tcgcgcgctg caaacgcgcg ttgtcctgac gaccacttaa caccagcagc
     7561 agattatgca ggcaaagcac ttcctgattg gttgggcagg cgcgaagact ttgttggcaa
     7621 aaatccagac cagcgtcgaa gttaccatta cgcgccgcca gtcgtgccag gccataatag
     7681 ccaccggctt tgctgttgcc gctccagacc gcacgccaga aatcctcttc ggcttgttga
     7741 tattgtccct gacgttcgta agcactggcg cgaatcaaac ttgcttgtcc acactgcggg
     7801 tttttgttca gcgcatgtgc gcgtttcagc gcctgactgg catacgccac cgcttgcggg
     7861 aaatctgcgc ggttatattc cagcatcgcc agcgccaggt tacagcgata atccagcggg
     7921 tccagcgcca cgccgcgcag gtagtaatcg aacggtgaac ggctggcgtg atgatattgc
     7981 tccagatgct gaccgataaa ccaggcttca tctgtactgg taatgtcttg tgctgccagt
     8041 ggcgctttgg cgacgtccgg taacggcaac gcttgcggct gatgttcctg ataactcagt
     8101 acaatattgc cgtcggcatc ggaaagctca atggtcagcc tatcaggatt gataccgtgc
     8161 aacacgccct ggatggcggt cgcaggcatc agtgccacgg catcatcaag taacgcgttg
     8221 catttgccga tttcgtggat cgccaggcgg tatccgttca acggagagat ggcatacagc
     8281 ccccactcaa tcccctgctc actacgctgg agttttatca ccgcatcgcg ggaggcattt
     8341 tgcaccatgc ccagagaatg ataaggcagg aaatactgct caaaacgctt ctcttcgtaa
     8401 gcatcaagcc aggtaaaatc gggctggtta tcggcaaaaa taccggtcat cagttcgata
     8461 tacgggccgt tattgtcggt cagactctta tcccacgcct ggccaaattc actgtgtccc
     8521 caactccact gttttttacc tggcgcaata tggtggttgg caacgtgcag caaaccgcca
     8581 tcttcatcgt gacaccacgc gccgacaaaa tcgtactgcg atttttcggc catatatgag
     8641 gttggaacgg gcacattttt atagcgagaa atgtccactc cagcggagta gtccactttg
     8701 tagtaagtgc cggtagcgat ggggaaagcg gagacggccc gtttgccgtg atcaaacacc
     8761 gcagtcaaat ccggtgggaa aacgctctga tgcccttcac cccctttcac tgccgggttg
     8821 gcccaccaca agaaatgacg cggcgtggcg ttgccgttat agacgcggct ggcgatttcc
     8881 agcgccgccc agggcgcagg gtgaaacctg tcatcacctg taaaccatgc atcggctccg
     8941 tttcgcctac ccacaccgtc tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg
     9001 gcataaaggt ggtcgggcga tgatgttgcg gccagttaaa ctcaatccca ccggaaatcc
     9061 acggccccag cagccccacc agcgcaggtt taatgacttc attgtgataa acaaaatcgc
     9121 gctgtttcac tttatcccac gcgcgatgca cccgaccgcc cagttccggc aggatcatca
     9181 ctttgatgta gtcgttttcc agccacaccg cctgccagga tttcagggtt ttctgctcgc
     9241 tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga cgatccctga taaacgcgat
     9301 tttccaggaa catgggatgt atatcctgcg gcccggtttc ataggtcggg atctcaacgc
     9361 gctcttgcca cacttttact ggagtcatgg tgccctcaat attaacaaga catactgaat
     9421 taaaagattt gtggcagtgt attgaacaat ctggcaatgt tttcgcggaa taatcacgca
     9481 attaactaaa caaggtttag tgaagatgag agcctgcatt aacaatcaac agattcgcca
     9541 ccataacaaa tgcgtgattc tggaactgct gtaccggcaa aagcgcgcca ataaatcaac
     9601 gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt aatattttgc aggaactgga
     9661 aagcgaaaaa cgggtggtga atatcgacga tgaaagccag acgcgcgggc atagtagcgg
     9721 tacatggctg attgcgccgg aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag
     9781 tattgagtgt caggttgcta atgcttgttt aagtccgaaa ggtgaatttg agtatttaca
     9841 gattgatgca ccgacgccgc aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca
     9901 ccgtaaattg tggccggacc gcaccatcaa tctggcgctg gcaatccacg gtcaggttga
     9961 tccggtgacc ggcgtgtcgc aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt
    10021 gaagtatctg ctggaagaga agctcggcat tcgggtgatg gtcgataatg actgcgtgat
    10081 gctggcgctg gcggagaaat ggcaaaataa ttcgcagaaa cgggatttct gcgtgatcaa
    10141 cgttgattac ggcattggct cgtcgttcgt gattaacgag caaatttatc gcggcagttt
    10201 gtatggtagc ggacagattg gtcacaccat cgttaatccg gatggcgtcg tctgcgactg
    10261 tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg
    10321 ggtatggcta aaatcacaac cggttaatac tcagcttgat ccggaaaaac tgactacagc
    10381 gcagttaatc gctgcctggt agagtggaga accgtggatc accagttggg ttgatcgcag
    10441 tgccaatgcc attggtttga gtctgtataa cttcctcaac atcctcaata ttaatcagat
    10501 ttggttgtac ggtcgcagtt gtgcctttgg tgagcactgg cttaatacca ttattcgcca
    10561 gacgggattt aacccgttcg accgcgacga agggccgagc gtgaaagcaa cgcaaattgg
    10621 ctttgggcaa ttaagccgcg cacaacaggt gttggggatt ggctatttgt atgttgaggc
    10681 gcagttacga cagatttgat ggcgcaataa cgtagaaagg cttcccgaag gaagccttga
    10741 tgatcacata cctaagattg cctgatgcgc tacgcttatc aggcctacac ggatattgca
    10801 atatattgaa tttgcaaagt tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    10861 aacaacgagc acattgacag caaatcaccg tttcgcttat gcgtaaaccg ggaaacgtgc
    10921 gcagatgtcg agaactttac ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc
    10981 cagcacgtca cacatccagc cagccagttc tttcgcttct gcttctttga agccgcgacg
    11041 cgtaatcgcc ggagtaccca cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt
    11101 cggtacgctg tttttgttga cggtgatgtt agcacggccc agagcggcgt ctgcttcttt
    11161 accggtcagg tttttatcaa ccagatcaac caggaacagg tggttatcag tgccgccgga
    11221 aaccactttg tagccgcgct caaggaacac ttctaccatc gctttagcgt ttttcgcgac
    11281 ctgctgctgg taagttttga actcaggctc catcgcttct ttcagagcaa ccgctttacc
    11341 ggcgattacg tgcatcaacg gaccgccctg accaccaggg aaaacggcag agttcagttt
    11401 tttgtacagc tcttcgctac cacctttcgc caggatcagg ccgccgcgcg gacccgccag
    11461 ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg ggtagacgcc
    11521 agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac aggtaagcac cgatgctgtc
    11581 agcgatttca cgcattttcg cccagtcaac cacgccggaa tatgcagaga aaccaccgat
    11641 aatcattttc ggcttgtgtt ctttggcttg tttttccaga tcggcgtagt cgatatgacc
    11701 ggtagcatcg ataccgtaag gaacgatgtt gtacagttta ccggagaagt taaccggaga
    11761 accgtgagtc aggtgaccgc catgcgccag gttcataccc agaacggtat cacctggttc
    11821 cagcagcgcg gtgtagaccg caaagttagc ctgggagccg gagtgcggct ggacgttagc
    11881 gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac
    11941 atactcgcaa ccgccgtagt agcgtttgcc cggataacct tcagcatatt tgttggtcag
    12001 ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc
    12061 gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt cggcatcata
    12121 atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct aacaataaaa
    12181 tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc gaatcattgt
    12241 gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttcaggc tacgcaaggc
    12301 tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg tgatgcaaat
    12361 ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat tgcagggcta
    12421 ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaagga agaccatatg
    12481 cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg
    12541 ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa
    12601 atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt
    12661 gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc
    12721 gcgcagaagc acaccagctt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac
    12781 ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt
    12841 aaagcctatg gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa
    12901 aacgccagca aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca
    12961 ccgcgcagcg cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca
    13021 gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag
    13081 gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg tattgcggtg
    13141 aaacgcgaag agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat
    13201 gtcgtgaaac tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca
    13261 gtgacgttaa tctctgccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg
    13321 gcaaaagcag gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgatgtt
    13381 cacgcctttg ccgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac
    13441 acctggcatc gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt
    13501 ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc
    13561 tgcggcccgg ttggcttcat gcagtttacc gcgaaacagt tagtggatct gggcgtgaag
    13621 caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtaatt tgatgttgcc
    13681 ggatggaaac atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg
    13741 gtacggatgc gaatgacccg tgccacgtca aagacgaaga ttttaccgtc accgattttg
    13801 ccggtttgcg ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg
    13861 acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc
    13921 tcggtatggc ctttctggcg accaaagcct ttcacttcgg tcaccgtcat gccggtaata
    13981 ccgacttcgg ccagtgcttc gcggacatcg tccagcttga agggttttat aatcgcatca
    14041 atctttttca tgctattcct tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac
    14101 ataacgccaa ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta
    14161 taaaattctg tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg
    14221 atttgcagca gcttacgcaa atagttgagt tcaaactgat tacgtgcctc aacaaaggtt
    14281 ggcagcgccg tattttcacc ctccagcgcc tgctccacca gcgcatcact aatcaccgga
    14341 gatgaggtca gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc
    14401 cagctcgcgg tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta
    14461 tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc
    14521 tccgccagtg ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg
    14581 aattccccgc gcgccatcgc ttttggcaga tcacggtgag tggcagaaat aatccgcaca
    14641 ttgatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc
    14701 agcagtttga cctgtaacgg cgcaggcata tcgccaattt catcgagaaa tagcgttccg
    14761 ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc
    14821 gcatgaccaa acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca
    14881 ataaatggtt tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc
    14941 ccggtgccgc tctgaccgtt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc
    15001 tgttccagca aacgcagcat cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca
    15061 tcggtggctg gcgcggattg ctccagcgca tcgtcaattg cctgatatag cgcatcttta
    15121 tcgacaggct tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg
    15181 atagaaccat gcgcagtaag aataattact ggcattcccg gctgcacttt ctggatttca
    15241 gcaaatagct gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact
    15301 ttttcgcgat tcagtacccg taatccttca gcgccacttt ccgccgtgac cacactgtag
    15361 ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat
    15421 aataaatgcg caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt
    15481 ttcactctcg tgcggcgtat ccggcgagaa atttccagtc ggtttgcggg tcgagagctg
    15541 gcgttcaata tcggtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag
    15601 aacgtgatgt tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact
    15661 gtaacgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg
    15721 atacagtggg cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag
    15781 ctggcggcgc tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt
    15841 attttgccag ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa
    15901 acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc
    15961 ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg
    16021 aattttttct tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacccag
    16081 caatgccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat
    16141 gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc
    16201 tggacatgga cagcgcggag aaaccggagc gtacatatcg tacgtgaaga tttcgagcac
    16261 tgcccggggc cgaactgaca aataaaatag cctgatagga taggctgtta gcattatttc
    16321 gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc
    16381 agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg
    16441 ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg
    16501 gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca
    16561 ccatgtgaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg
    16621 gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct
    16681 ttgagatcga cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc
    16741 actgtctcca ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt
    16801 ttacggttgt aatcaagcag ttgttcgatc agtttttgca aattgcggct gctgctatca
    16861 agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg gtcagccagt
    16921 aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt
    16981 aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt
    17041 tgcccaaccg agcgtaactc gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc
    17101 ccttccccca gacggttgat catgcgctcg atatttttca ccggcccgat aatcatccgc
    17161 gtgaaaagta gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa
    17221 tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg
    17281 cgcgtggcct gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg
    17341 gcagcatcgg gaccactgtt gttacactga agttgggcca gattgttcaa atcctgacgc
    17401 aatgcctggt aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg
    17461 taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac
    17521 tgacggtagc tacgctccat ctccagcgcc gcgttggtca tcgcttcact acgccgggca
    17581 tcaataagcg tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt caggctttgc
    17641 caggcttgcc atgccagcac caacaggggt agtagaatta gcaaaaatgc cagcattacc
    17701 agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac
    17761 gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg
    17821 gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct
    17881 acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta
    17941 gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgcttact attatgcagt
    18001 aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgagggat
    18061 ttatggcttc ttcgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact
    18121 taagataaac ttattagatt acaataaaat caatcattta agtgtcgcca gaaagcgaca
    18181 cggtaaacca cccattgtcg tgatttacag acacaaaaaa gctcccggag ttgggagctt
    18241 atgatagtgg ttggtgctta atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt
    18301 accccaactg cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt
    18361 tttccggatg ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa
    18421 tggtgactcg accactttca gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt
    18481 tagccgggta ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc
    18541 ctttgctttc cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat
    18601 gagagacagc aatcggcatt tgcgagccca ccatcccctg caacagcaga gacgggcttt
    18661 gggttacttc aaccaggctg aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac
    18721 gtggccacaa ctcgctcccc gggatcagtt cacgcaggtt agacatcatc tggcaaccgt
    18781 tacatacccc cagcgccagc gtttgcggac ggtggaagaa ggttgcaaac tcatcgcgta
    18841 cacggtcatt gaacaggatt gacttcgccc aaccttcacc ggcacccagc acatcaccgt
    18901 aggagaaacc accgcacgcg accagggcgt ggaaatcttc caggcccgtg cgtccggtca
    18961 gcaggtcact catatgcacg tcgatagcat caaagcctgc acggtggaaa gctgccgcca
    19021 tttcaacatg cgagttcacg ccctgctcac gcagcacagc aactttcgga cgtgcgccag
    19081 tggcaatata cggtgctgcc acatcttcgt tgatatcgaa cgacagtttc acattcaggc
    19141 ccggatcggc gtcgtttgat ttcgcctggt gctcctggtc agcacactcc gggttgtcac
    19201 gcaggcgctg catctgccag gtagtttctg cccaccagac acgcaacgtg gtgcggcttt
    19261 cgctgaatac agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc
    19321 ctacataatg gacacaatca gcaagcccat gctgtgccag tacggactcg accgcttcac
    19381 ggtcagcggc acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc
    19441 gatcgtcgcc cagagaggca atatccgcgt taatgccgca atgaccagca aaggccattt
    19501 ccgccagcgt taccagcagg ccgccatcag agcggtcgtg gtatgcgagc agcttacgct
    19561 gtgcaaccag cgcctgaatc gcgtcataga agcctttcag ttgcgcgacg tcgcgtacat
    19621 ctgccggttt gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt
    19681 tattgccttt gcccaaatca atcagcagca gtgtgttatc ttcggtagaa agctgtggcg
    19741 tgatggtgtg acgcacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg
    19801 acgtcatttc gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt
    19861 tacccaccgg gatcgtcagg cccagcgccg gacaaagctc ttcgcccacg gctttaacgg
    19921 cttcatacag gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa
    19981 gtttaatgcg tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga
    20041 ccgccagacg ggcagaggcg gcgaaatcca gcagtgcaac cggcgcacgc tcgccaatcg
    20101 ccatcgcttc accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg
    20161 gcacctgcca cggccccacc atctgatcgc gcgctaccat gccggttacg ctgcggtcgc
    20221 cgatggtcac caggaaggtt ttctctgcca cggtcggcag atgcagtacg cgtttcaccg
    20281 cgtctgcaat ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta
    20341 catcgcgggt catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct
    20401 gattatcaaa atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca
    20461 ccgcgtaggg tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag
    20521 caaccgccag cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc
    20581 ccggttcgtc gcttagaata tcgcgcagtt caaatttacc gccgcgcccg ccgtcgctca
    20641 ccagttccgg catcgcgtta gaaagaccgc cagcgccgac gtcgtggata aacaggattg
    20701 ggttggcatc accaagctgc cagcaacggt cgatcacttc ctggcagcga cgctccatct
    20761 ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc atcagactga cctgacgcca
    20821 tggaagacgc cgctccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca
    20881 gcttcgcacc gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc
    20941 cgcccgccag catgatcggt ttgtgataac cgcgcagctc ttcgccgttg tggctgttca
    21001 ctttttcttc ataagtacgg aagtagccat tcagtgccgg acgaccaaat tcgttgttaa
    21061 acgccgcgcc gcccagcggg ccttcggtca tgatgtccag cgcggtgaca atgcgctcag
    21121 gcttaccgaa atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggaaacgg
    21181 agaaaccaac cagaccagcc ttcggcttcg caccgcgtcc ggtggcgcct tcatcacgaa
    21241 tttcgccgcc ggaacccgtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt
    21301 gagtttcgac tttcatcaga atatgcgccg gttcctgatg gaaatcgtag cggcccgttt
    21361 cgtggtcagc aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat
    21421 aagcagagag aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc ttgaacagcg
    21481 atttcggctg ctgttcaccg tcgataatcc agtcggcatt aaaaattttg tggcggcagt
    21541 gctcagagtt cgcctgagcg aacatataca gctcgatgtc gttcgggtta cgaccaagct
    21601 ttgtgaaagc atcctgcaga tagtcaattt catcttccgc cagagccagg ccaagacgca
    21661 ggttagcgtc gatcagcgcc tgacggccct gccccagcaa atcaacgctg gtaaccggag
    21721 tcggttgatg gtgggcaaac aactgctctg catcatctaa agcaaaaaag accgtttcca
    21781 tcatgcggtc gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg
    21841 cttctatata gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat
    21901 gggcaatatc ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca
    21961 gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg gccatatttc agcaggcgtt
    22021 caagttgtgc gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact
    22081 cggcgtaaat attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga
    22141 ttcggaatgc cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt
    22201 ctaagctttc ggtgtaccca agggggggaa acgggcgtca ttataaagaa tctgatgcgc
    22261 tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg cgtgccgcca
    22321 atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc atttatgagt
    22381 aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca gagaattaac
    22441 taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact gctgctcgcg
    22501 gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat cgccgccatt
    22561 caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta taacgaaatc
    22621 aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga ttacctcggt
    22681 gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga cctcgataat
    22741 ggtaacgccg acctgctggc ggcaggactg gtctataaca gtgagcgggt aaaaaattat
    22801 cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt gagtcagtat
    22861 cgcccgcgca cactgggcaa cctgacggcg gagcaactca ccgttgcacc gggtcacgtg
    22921 gtggttaacg atctccaaac cctgaaagac acaaaattcc cggaattaag ctggaaggta
    22981 gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa actcgattac
    23041 accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga gctcgccgta
    23101 gcgctcgata tcaccgatga acaaccggtg acttggttta gcccgttaga tggcgataat
    23161 accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagactg tacgctggca
    23221 cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga tacgcgcaca
    23281 tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga gaaatacgcc
    23341 gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca ctgggatgca
    23401 caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa
    23461 agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg cgtgcgttat
    23521 ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgagcg gatctggttt
    23581 gccctcgctg cgtacaatat gggctatgcg catatgctgg atgcccgcgc cctgacggca
    23641 aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc tttacttagc
    23701 cagaaaccct attacagcaa gctgacttac ggctacgctc gtggacatga agcctacgct
    23761 tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca agagaaagag
    23821 aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt atcgcctacg
    23881 gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc atcaaactat
    23941 ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa acaaaattaa
    24001 tccgtcgagg gttgcgcttt tttctgcgct ttaatttcct ggcggcgcat gcgaaagaag
    24061 tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc cactcggtga
    24121 ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt tttcgcgtca
    24181 cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc acacattaca
    24241 catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg catcaccaga
    24301 ccaccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatggcg accaatcggg
    24361 cggttccagc cttcgccgat tacccgattg ttatgcacta ataccgcgcc gaccggcact
    24421 tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca gtattcgtgg
    24481 ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg cattatacac
    24541 ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt tactcgccaa
    24601 cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata gccactgtac
    24661 agccgcagcg gagtgccgat tttgcgctcc agttgtgcga ccttttcatg tcccagacaa
    24721 cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat aagattaacc
    24781 cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg agagccggta
    24841 atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc
    24901 ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa cgtctgtaaa
    24961 cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca gactcatcgg ccagcgtgcc
    25021 gcacgacctt ttatcaataa cgcaatggct ataatcggta acaacggcag gacaagtaac
    25081 gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat atcctgctga
    25141 tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc caaaccatac
    25201 tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac agatagatca
    25261 tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa ttcatggcaa
    25321 ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg ttgtcgccac
    25381 tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg ttaccagggg
    25441 cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga tacggcgcgc
    25501 catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag
    25561 ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt gagtgaagcg
    25621 ctggcaagcc agccggaatc gccctccgtg cccattcata accaaatccg cggtgatgat
    25681 ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat gtacgcaacg
    25741 ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg
    25801 cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag
    25861 ctgatgaaga ttggcttcaa tgctgccgca gtgcgcgata tgcatgcgct actcgcaaca
    25921 gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg tgtacgacgc
    25981 gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc
    26041 ggctttactc cgaatgccct gcaacagcgt gcttctcatt gcctgtatac cattgccgaa
    26101 gaacaagcga caaacagtgc ttcaatctct gcttgtcacg ctcagggaat gttaacggat
    26161 ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg tattcgtcat
    26221 agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtacaat gcccgcccgg
    26281 tttgtgttgt tttgagagtt tccttatggc gttgttaatc actaaaaaat gcatcaattg
    26341 tgatatgtgt gaaatcaaat aaaaacacat ttattttcat agtgatacag caatgtgtta
    26401 ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc ataagttcag caacacaaag
    26461 aacaccactt tctttaacgt cctccgcgcc ctaaaggacg gggctttacg gcgcaaaagg
    26521 cctgtgacat accacattaa cttttcagtc aaccgtagca cgttcttgta tacgccgtgt
    26581 ctgcggtttt atataaccct ggctggaaac ctcttataca aaaaagtcga ttcgccaaca
    26641 tcatagatga tcgccacctt ctggcgagaa actcctgatg caattagtcg cccggcctgc
    26701 gcccattatt ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg tgcgcgagca
    26761 gcttccagtc ctgcttttgt tcgttcaaca atcagttcac gttccatttc agccagggca
    26821 cccatcacat ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga atcagtcaga
    26881 ctacgaaagt tgatgcctcg ttcgcgcaac tcctccacca gcacgacaag atgccgcata
    26941 ctgcgcccca gccgatccag cttccagaca accagtgtgt cgcctgccga taatgtcctg
    27001 agcagttttt tcagccccgg cctttcggac tttgtgccac ttatcttgtc ttcaaaaatc
    27061 agctcgcatc ctgcacagtt caacgcatta cgttgtagat cggtattctg gtcatttgtt
    27121 gatacgcgta catagccaat aagcatggta aa
//
LOCUS       DAZOYS010000034        27108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOYS010000034 DAZOYS010000000
VERSION     DAZOYS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.034"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..925
                     /locus_tag="ACHUN9_001284"
     CDS             <1..925
                     /locus_tag="ACHUN9_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0694732.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHUN9_001285"
     CDS             complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHUN9_001285"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0694733.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHUN9_001286"
     CDS             1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHUN9_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0694734.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3886..4281
                     /locus_tag="ACHUN9_001287"
     CDS             3886..4281
                     /locus_tag="ACHUN9_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0694735.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4301..4891
                     /gene="diaA"
                     /locus_tag="ACHUN9_001288"
     CDS             4301..4891
                     /gene="diaA"
                     /locus_tag="ACHUN9_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0694736.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4901..5476
                     /gene="dolP"
                     /locus_tag="ACHUN9_001289"
     CDS             4901..5476
                     /gene="dolP"
                     /locus_tag="ACHUN9_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0694737.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5590..6630)
                     /locus_tag="ACHUN9_001290"
     CDS             complement(5590..6630)
                     /locus_tag="ACHUN9_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0694738.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6703..7338)
                     /locus_tag="ACHUN9_001291"
     CDS             complement(6703..7338)
                     /locus_tag="ACHUN9_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0694739.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHUN9_001292"
     CDS             7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHUN9_001292"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0694740.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7964..8407)
                     /locus_tag="ACHUN9_001293"
     CDS             complement(7964..8407)
                     /locus_tag="ACHUN9_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0694741.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHUN9_001294"
     CDS             8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHUN9_001294"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0694742.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8747..9250)
                     /locus_tag="ACHUN9_001295"
     CDS             complement(8747..9250)
                     /locus_tag="ACHUN9_001295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0694743.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9244..9768)
                     /locus_tag="ACHUN9_001296"
     CDS             complement(9244..9768)
                     /locus_tag="ACHUN9_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0694744.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9977..10972
                     /locus_tag="ACHUN9_001297"
     CDS             9977..10972
                     /locus_tag="ACHUN9_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0694745.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10981..11859
                     /locus_tag="ACHUN9_001298"
     CDS             10981..11859
                     /locus_tag="ACHUN9_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0694746.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12065..13072
                     /locus_tag="ACHUN9_001299"
     CDS             12065..13072
                     /locus_tag="ACHUN9_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0694747.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHUN9_001300"
     CDS             complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHUN9_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0694748.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHUN9_001301"
     CDS             complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHUN9_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0694749.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHUN9_001302"
     CDS             complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHUN9_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0694750.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHUN9_001303"
     CDS             complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHUN9_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0694751.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHUN9_001304"
     CDS             complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHUN9_001304"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0694752.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHUN9_001305"
     CDS             complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHUN9_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0694753.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHUN9_001306"
     CDS             complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHUN9_001306"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0694754.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHUN9_001307"
     CDS             complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHUN9_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0694755.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHUN9_001308"
     CDS             complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHUN9_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0694756.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHUN9_001309"
     CDS             complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHUN9_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0694757.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHUN9_001310"
     CDS             complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHUN9_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0694758.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26830..26906)
                     /locus_tag="ACHUN9_001311"
     tRNA            complement(26830..26906)
                     /locus_tag="ACHUN9_001311"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26870..26872),aa:Met,seq:cat)
BASE COUNT         6423 a         7517 c         6809 g         6359 t
ORIGIN      
        1 tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct ctgcgggtaa
       61 ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac atcagacagg
      121 atattacaaa ctcaatgaca gcctggatat taaaaccaca ttacaggcaa acgacattcc
      181 aggactcaca accgaccagg ttgtctctgt taacacccga ttcacacaga taaaaagcag
      241 caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt ctcgttatgg
      301 acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc catttctcgg
      361 ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg tcgatggaat
      421 gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgattc agggaaatct
      481 caccgcccca cagtcgtgca aaattaatca gggcgatgtt attaaggtta attttggatt
      541 catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta ctccagtaga
      601 ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt tgcaaatgcg
      661 catcgacggt acaactgggg tagtagacca gtacaacctg gtcgccaggc gaagaagttc
      721 agacaatgtg cccgatgtcg gtattcgtat tgaaaatctc ggcggcggag ttgcaaatat
      781 tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg tcaatatgcg
      841 cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc agggcaccgc
      901 caccattacc gtcatggtgc ggtaaaagaa gacgttgcgt tttgattaac gcaacgcttc
      961 ggcactgtta cccctgctgc tccagcgcat acttatacag cgcatttttc ttcacgccat
     1021 gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt agcagcgcca
     1081 gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc tttatgacct tcgacaatca
     1141 gcaccatttc gcctttgcga cggttttcat cttcctttac ccacgccagc agctcgccaa
     1201 cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc acgcgccaga accacgtagc
     1261 gggattcgcc taataccgca acgatatctt ccaggctatc taacagacgg tgggtagatt
     1321 cataaaaaat cagcgtgcgc ggctccgctt caatggcttt tagcgcatca cggcggcctt
     1381 ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa cccgcggcgc
     1441 ttaacgcagt gatagcagca cacggtccgg gtagcggcac cacacgaatc cccgcttcac
     1501 ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg gcatcggaaa
     1561 ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct ttttgttgtt
     1621 cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc agcaataaac
     1681 cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc tctaacgcac
     1741 gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc tggccctgag
     1801 aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt taatattgag
     1861 cattgcgtaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     1921 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca
     1981 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     2041 tcttcagcag atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat
     2101 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     2161 gcaagaactg aacgattctc agcgtcgcga gaaaacactg ctggcggtag agattaaact
     2221 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt
     2281 agaacaaaac cagcaagcgc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc
     2341 ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggcg cgaaagaaaa
     2401 aaagcagaat attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa
     2461 tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt
     2521 ctggtttgat aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcaaaa
     2581 acgttatccg aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc
     2641 gtttaaacca gcctcgacca acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc
     2701 ggtatttggt cgcaccattc agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc
     2761 agtggcggct caggtagctg ccgcacctgc cgcagacgta gctgaacaac ctcagccgca
     2821 aaccgtggat ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca
     2881 gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc
     2941 acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca
     3001 gatcttaagc caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa
     3061 taacgttgaa gagttgctga agagcaacac cccgctgaac gtgctggcac tgaaccagcc
     3121 agagaatatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcacctg aagacgaagc
     3181 gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc
     3241 acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaactggg
     3301 cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa
     3361 cggcggttct ggtatcgctt taacgggtac cccgattact cccagagcga caaccgactc
     3421 cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa
     3481 taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat
     3541 caaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaatgctgt acgccagctc
     3601 ccgcagcgca caaggcaccg caggcccgga tttccgtctg gagatggaag gtttgcagta
     3661 cagcgaaatc ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc
     3721 ggtgaataac gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct
     3781 ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg
     3841 aagcctgacg gcaaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca
     3901 acaaggtcag gtagtccccg tcagttaacc accaaacaga ccggcgatgc gtgggaagca
     3961 caagcgcgtc gctggctgga aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag
     4021 cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt tgtcgaggta
     4081 cgctatcgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa
     4141 cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact
     4201 gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat
     4261 gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc
     4321 ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat
     4381 ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg
     4441 tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt
     4501 cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac
     4561 ggcgattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg
     4621 tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa
     4681 agcagttgaa gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg
     4741 cggcgagctt gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg
     4801 tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga
     4861 taacacgctt ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat
     4921 cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac
     4981 cgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga
     5041 tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga
     5101 aacccgtatt aatgtcacgg cgtatcaggg caaagtgctg ctggttgggc agtcaccaaa
     5161 cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt
     5221 gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg
     5281 gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt
     5341 gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc
     5401 gaaagcggcg gcagatattg ccagccgggt gagcggcgta aagcgggtaa ccacagcgtt
     5461 tacgtttatt aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca
     5521 ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt
     5581 tccctcaaat cagaataaca gcgccagccc gccgacaatc acgcctgaca ctgccaccat
     5641 cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg
     5701 caaactcact gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc
     5761 cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc
     5821 tgctaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg
     5881 gaataaccag acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt
     5941 actccagaaa agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc
     6001 cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac
     6061 cagcgtcgca atcagcaaca ccatcaccag cccggccacc agacgaatcg ccgcaaaacc
     6121 ccagccgagg acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat
     6181 ccagaatgcc agcgcaccgc ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg
     6241 agccgcacag caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc
     6301 gcgaaagcgc gattgtccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat
     6361 caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga aatagatcat
     6421 cgcgtaatcc aacgccgcct gccacggatt agcatccgcc tgcgcaagga tagatttacc
     6481 gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc atttcacata
     6541 ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt
     6601 tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt
     6661 gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg
     6721 taattctgaa gagcttaaaa tagtcacgcc ttcatgctca ggccgcatcg cctctgccag
     6781 catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggca gtaaacgaaa
     6841 cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gcaacatcga
     6901 cgggcgagca atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc
     6961 tttgacgcgg ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag
     7021 catatgctgc gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc
     7081 ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa
     7141 cacaatgtcg atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg
     7201 attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac
     7261 tttcggttcg ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt
     7321 aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct
     7381 catgcaccag gcttaataga ccatgaggta attatccccg attgtgtgaa attcgcctca
     7441 tccaatgcaa caacgcggag gaagcatgag taagaaaatt gccgttttaa tcagtgatga
     7501 atttgaggat tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt
     7561 gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt
     7621 gaccatcgat aaatccatcg atgaagtgac gccagcggag tttgatgccc tgctgctacc
     7681 gggtggtcat tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga
     7741 ttttgtgaat agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag
     7801 cgccgatgtg attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa
     7861 aaatgcgggc gcggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac
     7921 cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc
     7981 ctgagtcgcg tagccaaatc atttttttac caaagcccag cgtgttgtca gtgaatttga
     8041 tttcatcgag ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac
     8101 gattgtacgc ctggcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt
     8161 taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa
     8221 ccgcagcctg cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga
     8281 aggcgacttt ctgcgcatca aacagataaa aagcattagc gcaccacagt tccccttcct
     8341 gctgcacaca ccaggtgaca acatgttgtt ttgccaacca acggctgatg gtgatgagtg
     8401 tttccattgc tgctctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg
     8461 acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc
     8521 acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc
     8581 gggaaaggag aactgacgct ggcgttttct gcgccagtgg gcgatcgttc gctggcgtta
     8641 cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa
     8701 ggcgcagggt ttgcagagct gttaagcagt ctgcaaaccc cggagattaa aagcgattga
     8761 aatgctcgtg atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat
     8821 gtacctggaa ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca
     8881 gttcaaagcc gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc
     8941 cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac
     9001 ggtatttttc atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca
     9061 catcaaccgg actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca
     9121 gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac
     9181 tttcgaatga gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc
     9241 gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc
     9301 ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag
     9361 ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc cttcaatcac
     9421 cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag
     9481 atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt
     9541 gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca
     9601 gcggccttca agaaactcca gctcaccatc atccagcgcc tgacggaatt gccagcttaa
     9661 gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa
     9721 cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc
     9781 ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa
     9841 caattccata tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat
     9901 tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgaaagcgca
     9961 accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc
    10021 ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata ctaatgcccg
    10081 tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca
    10141 tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc
    10201 ccgttggcag cgcgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc
    10261 cgacctcgcc atgctggagt acgccgccga gcgttacccg catattgagc gtcatgtgtc
    10321 agtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgatgt
    10381 tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt
    10441 cacacctgtc ccgctggaag tctttgcttt tggcagcctg tgcattatgt cggaaggtcg
    10501 ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc
    10561 ggcccgtttc gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt
    10621 gctgatcgac cgttatcagg atggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg
    10681 ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct
    10741 ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca
    10801 gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg
    10861 taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat
    10921 gtccgaaggc acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca
    10981 atgaaatatt ccttagggcc agtgctgtgg tactggccaa aagaggcgct ggaagaattt
    11041 tatcagcagg ccgccaccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc
    11101 aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagc
    11161 gggaagcaga tagtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa
    11221 ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg
    11281 aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac
    11341 gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag
    11401 ctttcccgcg actggctggt gaatctgctt aatcagtgcg ataagttggg gattcgcaac
    11461 cagtttgaag tggaagtcct gagctacggt catctaccgc tggcctactc cgcccgctgc
    11521 tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat
    11581 ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt
    11641 cagaccatga gcggctacgt ttacaacctc ggtaacgagc tgacatccat gcagggattg
    11701 gttgatgtgg tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc
    11761 cgcgctaatg aaaatggcgc tgcgccactg ccgttgacgg caaacagcga ctgtaacggc
    11821 tactggcggc ggctggctgg gctggagctg caagcctaag taaatatgct cgtcatactt
    11881 caagttgcat gtgctgcatc tgcattcgtt caccccagtc acttacttat gtaagctcct
    11941 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagcac agctcacttt
    12001 gttaacaact ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc
    12061 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca ttcccgaagg
    12121 ttcttcagcg cgagaagcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
    12181 cggctatcat cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc
    12241 cacgtcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
    12301 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa
    12361 tacactctat ccggggcgaa tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg
    12421 gacaatgatg gcgctgcgtc gccatatgag cggcgatatt gataatttcc cccgcgatgt
    12481 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
    12541 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact
    12601 ggcggcgcag cttggtctgc cgttcgcgtt tgcctcacac ttcgcgccgg atatgttgtt
    12661 ccaggcgctg catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata
    12721 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctatt
    12781 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccggac aactgccgcc
    12841 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatggcg tgcagcaggc
    12901 gctgagtatg tcgttggtgg gtgataaagc gaaagtgcgt cacggcttgc agtcgatcct
    12961 gcgcgaaacc gacgccgatg aaattatggt taacgggcag attttcgacc accaggcgcg
    13021 cctgcattcg tttgaactgg cgatggatgt gaaggaagag ttgttgggat agtgtgtctt
    13081 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtagacctga taagcgaagc
    13141 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
    13201 ccggcagcaa attaaagctc gataagatat ggaccagtgc gttgccaacg ccaaacacca
    13261 gaatcagcgt aatcatcggc ttgccgcccc agacacggaa tttcgggctg ccaaagcgtt
    13321 tacgcgatgc gcgggctaac agcgccggaa caattgccgc ccagatggta gctgctaaac
    13381 cagcataacc aatggcgtac aggaatccgt tcgggaacag tagaccaccc acaacaggcg
    13441 gggcaaaggt cagcaatgcc gttttcaagc gccccagggc tgagtcatcg aaaccaaaca
    13501 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
    13561 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
    13621 cctgtaccag cacatcaata ttaccgccct tctctgcaat accgataaac tccggacgcg
    13681 ggatgttacc catcgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
    13741 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggctcg
    13801 gcacgttacc gtgataacca aacgatgcca gacagaacgg cagggtcatc agcaaatacg
    13861 gcgcataaga tgcattgctt tcggcgacgt tgaacaatgt cgcaggctgc acatgcccca
    13921 gtaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagtaca atcgccgtca
    13981 tgcggctgac ggccttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
    14041 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaattg
    14101 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatac
    14161 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
    14221 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    14281 aggtaaagat cagcgccgcc attgaccaga aaaaccacgc cccggacatg accactggca
    14341 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    14401 acggtgacgt ttgggtggtg gttagtgttg ccatgagagc ttctctccag tgaaaaatag
    14461 tgcgactgcg ttgttatgca ttgcactgta ccagtacacg agtacaaaag acagaaaaaa
    14521 agccccgatg gtaaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    14581 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggag
    14641 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacggcca ccttcacggc
    14701 gttcaccgct gaagttacga ccgccttcac gacgttcgcc accgaaacca cgaccaccgc
    14761 cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    14821 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    14881 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc aatgtaacgg ctactgatgt
    14941 cgccttcgtt agcaatcgca ccaacgatat gacgaacttc aacaccatca tcgcggccca
    15001 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    15061 cacggtcacc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    15121 ggaattcacg tttcggacgc atcggcgcat ctggcggtac gatcagagta cgttcaccct
    15181 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    15241 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    15301 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    15361 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    15421 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    15481 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaactacc aggctgatac
    15541 gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat caggatgtcc agacgaccat
    15601 ctttcaggcg ttccagtgtc tgttcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    15661 cgctgttgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    15721 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    15781 ttttgcgcat accccagaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    15841 tggactgaat gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    15901 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    15961 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    16021 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    16081 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    16141 ggccgccgta cagagcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    16201 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    16261 tcagctcagg atcaagattc tgcaacagag gtaaagagaa tgctgcagtt tttccgctcc
    16321 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    16381 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    16441 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    16501 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    16561 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    16621 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    16681 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    16741 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    16801 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    16861 ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc
    16921 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    16981 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    17041 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    17101 cttgtcacga ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt agttgtaagt
    17161 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    17221 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    17281 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    17341 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    17401 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    17461 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    17521 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    17581 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    17641 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    17701 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttctagaac
    17761 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    17821 agcgatttga gagatgtgga ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    17881 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    17941 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcgatctt
    18001 cacagtaccg tcatcttcga tttcgatggt agtgccagtt tcttcggtca gggcacggat
    18061 aacagagccg cctttaccga taacgtcttt gatcttatcc gggttgatct tgatggtatg
    18121 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    18181 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    18241 ggtgatacct tcaattttga tatccatctg cagtgcagag ataccgtcgc gggaacctgc
    18301 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    18361 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgattgg
    18421 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaagaacc
    18481 gttggattcg gtgatttcag acactacacg tacggtgtac gggaatttgt ccatatccgg
    18541 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    18601 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    18661 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cggtacccag
    18721 cgttgcagta accagtgcct gcgtttcacc gcgggtgaac agcgcagaac cgtgagtacg
    18781 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    18841 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatag cgtgcagaat
    18901 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    18961 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    19021 actcagacga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    19081 cggctgccag tcccaacgcg gtttaccggc ttctttcact agttcattga tgttctgaat
    19141 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctgat cttcgctcag
    19201 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    19261 cagtttgctc tctttcagct cgtcctgagt cgggttcagt acgtactggt cattgatgta
    19321 acctacgcgg gcagcaccaa tcgggccatt gaacggaata ccagacagag acagtgctgc
    19381 ggaagcacca atcatcgcga cgatatccgg gttaacctgc gggttaacag aaaccacggt
    19441 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    19501 aatcagacgc gcgatcaggg tttcgccttc gcttggacga ccttcacgac ggaagaagct
    19561 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    19621 gtcctgacct ggtttggctt ttttctgtcc aacaacggta acgaataccg cggtgtcatc
    19681 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    19741 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    19801 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    19861 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    19921 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    19981 cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg
    20041 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    20101 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    20161 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    20221 aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    20281 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    20341 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    20401 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    20461 attcaaccac caggcgacga ggcgcgacgc ggccttcatc gtcaatttcg cccataccga
    20521 taaacttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    20581 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    20641 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    20701 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcaccatcc
    20761 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    20821 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    20881 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    20941 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    21001 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    21061 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    21121 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    21181 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgcctg
    21241 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    21301 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    21361 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    21421 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    21481 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    21541 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    21601 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    21661 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    21721 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    21781 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    21841 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    21901 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    21961 aagcgatggt acgttggatc tcaatgattt cgaatacttc gatcacatcg ccagtgcgga
    22021 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    22081 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    22141 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    22201 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    22261 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    22321 tgacggagta gtaacgcaga tccaagcttt ccgcttcaat cactttacgt gcagaggcat
    22381 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    22441 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    22501 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    22561 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    22621 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    22681 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    22741 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    22801 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    22861 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    22921 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    22981 gcagcaggat agcgtccagc agttcatcga tgccggtacc cgctttcgca gatacgtgta
    23041 cgaactggct ttcaccgccc cactcttccg gcaggatgcc atactgggag agttcgttct
    23101 taacacgatc cggatcggct tctggtttat caatcttgtt cactgcaacc accaccggta
    23161 cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg cggcatcacg ccgtcgtcgg
    23221 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc attgaagtaa
    23281 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    23341 atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggcaactttc gttgaacgaa
    23401 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    23461 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    23521 tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt gcggtttcct
    23581 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    23641 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatggtt tcgccgatca
    23701 caacgtcacg gttaacggcc tgagctggct tctggaagcc ttgctgcagg gaagaacctt
    23761 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    23821 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    23881 gaccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    23941 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    24001 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgatgcg
    24061 cttcttcttc ggctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    24121 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    24181 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcagcctca cgttcagctt
    24241 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctt
    24301 cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa
    24361 aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg gtaccaggaa
    24421 tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct gaatttttct
    24481 gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg tcagcagact
    24541 tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc tgtcgctctg
    24601 cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca gtttattacg
    24661 cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg gccttttcgt
    24721 cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga tcttccagcg
    24781 tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctacccct tcaaggttca
    24841 gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg
    24901 ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca aggccttcga
    24961 tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag aagccttctt
    25021 ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg
    25081 ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt
    25141 cccaaccgct cagctgcgaa gccagacgca cgttctgacc gttacggcca atcgcctgcg
    25201 ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc accacgatag
    25261 aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta tcatcccaca
    25321 ggacgatatc gatacgctcg ccacccagtt cagtagacac cgcctgaaca cgcgcgccac
    25381 gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc accgcgattt
    25441 tcgcacgaga acccggatcg cgagccgctg ctttaatttc aatcacttct tcgccgattt
    25501 ctggcacttc aatacggaac agttcgatca gcatttccgg cttggaacga gtgacgaaca
    25561 gttgcgcgcc acgcgcttcc gggcgaacgg aataaagcac gccacgaacg cggtcgccag
    25621 ggcggaagtt ttcacgcggc agcatatctt cacgcaggat cacggcttca gcgttgttgc
    25681 ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg atgatttcac
    25741 cttcgtgttc acggaactga tcaaccacca tcgcacgttc ggcttcacgc actttctgca
    25801 cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac tcaatctgat
    25861 cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct tcaagggtga
    25921 tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaag gtgtcaaaat
    25981 caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat tttttctttg
    26041 ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt
    26101 cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc ttttcacctc
    26161 atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc agcgcgaaca
    26221 cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct ttgataacgc
    26281 cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc tcttctccga
    26341 caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt gaggagactt
    26401 ccaggttata agcaacggtg atgggatctt caacatccag cacagcactt acctggtggc
    26461 tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag atgcgcagtg
    26521 tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg gcctcaactg
    26581 gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc cccaagacat
    26641 aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc ccgataaatc
    26701 ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact ttccagaagg
    26761 attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa aaaggactct
    26821 aagggaaagt ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac
    26881 gagctaccag gctgctccac cccgcgcctg aaacgtggta aattctactc gttttgggta
    26941 aaaaatgcaa atactgctgg gatttggtgt accgagacgg gacgtaaaat ctgcaggcat
    27001 tatagtgatc cacgccacat tttgtcaacg tttattgcta atcatgtgaa tgaatatcca
    27061 gttcactttc atttgttgaa tacttttgcc ttctcctgct ccccctta
//
LOCUS       DAZOYS010000035        26646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOYS010000035 DAZOYS010000000
VERSION     DAZOYS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.035"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            127..576
                     /gene="alaE"
                     /locus_tag="ACHUN9_001312"
     CDS             127..576
                     /gene="alaE"
                     /locus_tag="ACHUN9_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0694759.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(613..957)
                     /locus_tag="ACHUN9_001313"
     CDS             complement(613..957)
                     /locus_tag="ACHUN9_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0694760.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1109..1438
                     /locus_tag="ACHUN9_001314"
     CDS             1109..1438
                     /locus_tag="ACHUN9_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0694761.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1687..1932
                     /gene="nrdH"
                     /locus_tag="ACHUN9_001315"
     CDS             1687..1932
                     /gene="nrdH"
                     /locus_tag="ACHUN9_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0694762.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1929..2339
                     /gene="nrdI"
                     /locus_tag="ACHUN9_001316"
                     /pseudo
     CDS             1929..2339
                     /gene="nrdI"
                     /locus_tag="ACHUN9_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2312..4456
                     /gene="nrdE"
                     /locus_tag="ACHUN9_001317"
     CDS             2312..4456
                     /gene="nrdE"
                     /locus_tag="ACHUN9_001317"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0694763.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVQSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4466..5425
                     /gene="nrdF"
                     /locus_tag="ACHUN9_001318"
     CDS             4466..5425
                     /gene="nrdF"
                     /locus_tag="ACHUN9_001318"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0694764.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5781..6983
                     /gene="proV"
                     /locus_tag="ACHUN9_001319"
     CDS             5781..6983
                     /gene="proV"
                     /locus_tag="ACHUN9_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0694765.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6976..8040
                     /gene="proW"
                     /locus_tag="ACHUN9_001320"
     CDS             6976..8040
                     /gene="proW"
                     /locus_tag="ACHUN9_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0694766.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8098..9090
                     /gene="proX"
                     /locus_tag="ACHUN9_001321"
     CDS             8098..9090
                     /gene="proX"
                     /locus_tag="ACHUN9_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0694767.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9381..10565
                     /locus_tag="ACHUN9_001322"
     CDS             9381..10565
                     /locus_tag="ACHUN9_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694768.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10689..11426
                     /gene="ygaZ"
                     /locus_tag="ACHUN9_001323"
     CDS             10689..11426
                     /gene="ygaZ"
                     /locus_tag="ACHUN9_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0694769.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11416..11751
                     /gene="ygaH"
                     /locus_tag="ACHUN9_001324"
     CDS             11416..11751
                     /gene="ygaH"
                     /locus_tag="ACHUN9_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0694770.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11842..12372
                     /gene="emrR"
                     /locus_tag="ACHUN9_001325"
     CDS             11842..12372
                     /gene="emrR"
                     /locus_tag="ACHUN9_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0694771.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12499..13671
                     /gene="emrA"
                     /locus_tag="ACHUN9_001326"
     CDS             12499..13671
                     /gene="emrA"
                     /locus_tag="ACHUN9_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0694772.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13688..15226
                     /gene="emrB"
                     /locus_tag="ACHUN9_001327"
     CDS             13688..15226
                     /gene="emrB"
                     /locus_tag="ACHUN9_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0694773.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15290..15805)
                     /gene="luxS"
                     /locus_tag="ACHUN9_001328"
     CDS             complement(15290..15805)
                     /gene="luxS"
                     /locus_tag="ACHUN9_001328"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0694774.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15955..17511)
                     /gene="gshA"
                     /locus_tag="ACHUN9_001329"
     CDS             complement(15955..17511)
                     /gene="gshA"
                     /locus_tag="ACHUN9_001329"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0694775.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17584..18012)
                     /locus_tag="ACHUN9_001330"
     CDS             complement(17584..18012)
                     /locus_tag="ACHUN9_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0694776.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18009..18575)
                     /gene="yqaB"
                     /locus_tag="ACHUN9_001331"
     CDS             complement(18009..18575)
                     /gene="yqaB"
                     /locus_tag="ACHUN9_001331"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0694777.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18856..18932)
                     /locus_tag="ACHUN9_001332"
     tRNA            complement(18856..18932)
                     /locus_tag="ACHUN9_001332"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18896..18898),aa:Arg,seq:acg)
     gene            complement(19131..19207)
                     /locus_tag="ACHUN9_001333"
     tRNA            complement(19131..19207)
                     /locus_tag="ACHUN9_001333"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19171..19173),aa:Arg,seq:acg)
     gene            complement(19271..19347)
                     /locus_tag="ACHUN9_001334"
     tRNA            complement(19271..19347)
                     /locus_tag="ACHUN9_001334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19311..19313),aa:Arg,seq:acg)
     gene            complement(19351..19443)
                     /locus_tag="ACHUN9_001335"
     tRNA            complement(19351..19443)
                     /locus_tag="ACHUN9_001335"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19407..19409),aa:Ser,seq:gct)
     gene            complement(19759..19944)
                     /gene="csrA"
                     /locus_tag="ACHUN9_001336"
     CDS             complement(19759..19944)
                     /gene="csrA"
                     /locus_tag="ACHUN9_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0694778.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20179..22809)
                     /gene="alaS"
                     /locus_tag="ACHUN9_001337"
     CDS             complement(20179..22809)
                     /gene="alaS"
                     /locus_tag="ACHUN9_001337"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0694779.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(22937..23437)
                     /gene="recX"
                     /locus_tag="ACHUN9_001338"
     CDS             complement(22937..23437)
                     /gene="recX"
                     /locus_tag="ACHUN9_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0694780.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23665..24726)
                     /gene="recA"
                     /locus_tag="ACHUN9_001339"
     CDS             complement(23665..24726)
                     /gene="recA"
                     /locus_tag="ACHUN9_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0694781.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(24806..25303)
                     /gene="pncC"
                     /locus_tag="ACHUN9_001340"
     CDS             complement(24806..25303)
                     /gene="pncC"
                     /locus_tag="ACHUN9_001340"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0694782.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25448..26533)
                     /gene="mltB"
                     /locus_tag="ACHUN9_001341"
     CDS             complement(25448..26533)
                     /gene="mltB"
                     /locus_tag="ACHUN9_001341"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0694783.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         6284 a         7067 c         6667 g         6628 t
ORIGIN      
        1 aaaatttttc actaattgaa tagtcatagc cagcgatata cgctatgcga aaatgcagat
       61 ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt attaaggagc
      121 attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac gttcgcgatg
      181 gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg aatgagcttc
      241 gaacagtctt tttattccag attggtagcg attccggtga acatcttaat tgcatggcca
      301 tacggtatgt accgcgatct atttatgcgc gcggcacgca aagttagccc gtcgggctgg
      361 ataaaaaatc tggctgatat cctggcttat gtgacgttcc agtcaccggt gtatgtggcg
      421 atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag ttcaaacatc
      481 gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg ccgccgactg
      541 tttaaagtca gccattacca gcaggtaaaa gcctgactct tcttttcgcg actggcatcg
      601 ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt caagcgcaac
      661 acgcgaactg aagccatgcg ggataccgta atgctcgtgt cgctcgcctg ctaaatagag
      721 cggaaattgt tgataatgat cgcaagtttt aatatcggaa gcgggttcag ccagtgtcga
      781 actacgttct tttaatgttg gatgaattac cagtgcggta cgtcccattc gcgcttcgcg
      841 attaacatag acataatttt ccccacggcg gtaaccataa gctttctggg ttaccacatc
      901 gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta aatacatatt
      961 tgatcctcgt tattatcaac cgcgggccta ccttacctga ttgcgcattc acaacgcttt
     1021 cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc tacactgaaa
     1081 attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca acgacgttga
     1141 tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagcc tcgaatctgt
     1201 attgaagtcc tggggcagcg acgccaaagg ggaagctgaa gccgcacgca gtaaagctca
     1261 ggcattgctg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc agcaagccgc
     1321 acgcgatgcc gttggctgcg cggattcttt tgttcgtgaa agaccctggt gtagcgtggg
     1381 tacagcagct gcggtaggta tttttatcgg cgcactgtta agcatgcgca aatcgtagtg
     1441 caaaaatgat aataaatacg cgtctttaac cccgaagcct gtcttcgggg tttctttttg
     1501 cctggtgaat cacaaaaatc cccctccccc gtcacgctca tatctagggt aatttcgatc
     1561 actatttgct atatattgtg tggttgaatc tttttttcaa ctacatctag tatctctgta
     1621 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     1681 cgaatcatgc gcataactat ttacactcgt aacgattgcg ttcagtgcca cgccaccaaa
     1741 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     1801 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     1861 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     1921 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     1981 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     2041 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     2101 tgccacgata ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     2161 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     2221 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     2281 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     2341 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aacctctacg atagcgcagg
     2401 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttatggcga cgcatgtgcg
     2461 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     2521 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     2581 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     2641 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     2701 cacgatggtg gcgctgacgc tggcacaagg cgatgcgacg ctggcgttgc aactgaccga
     2761 tgaaatgctg tcaggacgct ttcagccagc cacgccgaca ttcctcaact gcggtaagca
     2821 gcagcgcggc gaactggttt cctgttttct gctgcgtatt gaagacaata tggagtcgat
     2881 tggccgggcg gtaaattccg cactgcaact gtcgaaacgc ggcggcggcg tggcattttt
     2941 gctgtcgaat ctgcgagaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     3001 cgtaattccg gtgatgaaaa tgctggaaga cgcattttcc tatgccaacc aactcggcgc
     3061 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     3121 cgatacaaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     3181 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     3241 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca tcagcgaaca
     3301 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     3361 cttccagcgg ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     3421 cacggtaaac cgtgctaacc ctgtcgccgg gcgcataaat atgagtaatc tctgctcaga
     3481 gattttgcag gtcaactgtg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     3541 ccatgatatc tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     3601 ttttgcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     3661 tatccgcagc gtgcagtcca tcgaagccgg aaatgccgcc tcacacgcca tcggactggg
     3721 gcagatgaat ttacacggct atctggcgcg agaaggcatc gcttatggtt cgccggaagc
     3781 actggatttc accaatctct atttctatgc catcacctgg cacgcactgc gtacctcgat
     3841 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gttatgccag
     3901 tggtgaatat tttagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     3961 cgaactgttt gcccgcagcg gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     4021 cgacgacgtg atgcgctacg gcatttacaa ccagaatctt caggcggtgc cgccaaccgg
     4081 ttctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     4141 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     4201 aaatctggcg ctgtatcagg atgcttacga aatcggcgca gaaaagatca tcgacaccta
     4261 cgcggaagcg actcgccatg tcgatcaggg tttgtcgctg acgctgtttt tccccgatac
     4321 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     4381 gctctattac attcgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     4441 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     4501 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     4561 accagaaaag gtgccgctgt cgaacgatat tcctgcctgg cagacattaa ctgtcgtaga
     4621 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     4681 tatcggcgcg ccttcgctga tgcccgatgc actcacgcct catgaagaag cggtattatc
     4741 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     4801 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     4861 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     4921 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     4981 ttccagccgc ggaaagctga ccaataccgc agacctgatc cgtctgatta tccgtgatga
     5041 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     5101 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     5161 cgagctgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     5221 gtttctctgt tacaacgcca ataaggcttt gatgaatctg ggctacgaac ctttatttcc
     5281 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     5341 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     5401 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcgaca
     5461 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attttgtggt gatctacact
     5521 gatactctgt tgcattattc gcctgaaacc acaatattca ggcgtttttt cgctatcttt
     5581 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg gaaattccgg
     5641 caatttgctc gcatcaatat tcatgccaca tttgccatca ggggttgcct cagattctca
     5701 gtatgttagg gtagaaaaaa gtaactattt ccattgggta atatatcgac ataaacaaat
     5761 aacaggaatc tttctattgc atggcaatta aattagaaat taaaaacctt tataaaatat
     5821 ttggtgagca tccacagcga gcattcaaat atatcgaaca aggactttca aaagaacaaa
     5881 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     5941 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     6001 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     6061 aaatatccga tgccaaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     6121 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     6181 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     6241 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     6301 gattagcccg cgcgttagcg attaatccgg atattttatt aatggacgaa gccttctcgg
     6361 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     6421 atcagcgcac cattgtcttt atttcccacg atctcgatga agccatgcgt attggcgacc
     6481 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     6541 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtat
     6601 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa acccctggct
     6661 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     6721 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     6781 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     6841 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     6901 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     6961 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     7021 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccgac tgacggcggt
     7081 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     7141 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     7201 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     7261 agtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     7321 atcgcctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtttc gctgattgcc
     7381 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaaccgcc
     7441 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     7501 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     7561 ctggtaccaa tcgtcatgct gtttggtatc ggtaacgtgc cgggcgtagt ggtgacgatc
     7621 atctttgctc tgccgccgat tatccgtctg acgattctgg ggattaacca ggttccggcg
     7681 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     7741 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggct
     7801 ctttctatgg tggtcatcgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     7861 cgcggtatcg gtcgtctgga tatgggcctt gccaccgttg gcggcgtcgg gattgtgatc
     7921 ctcgccatta tcctcgaccg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     7981 aaccgtcgct ggtacaccac tggccctgtc ggtctgctga cccgcccatt cattaagtaa
     8041 ctctgcactt gcccggtaac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
     8101 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgca
     8161 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
     8221 accttccaga cgctgctggt cagtcgtgca ctggagaaat taggttatac cgtcaataaa
     8281 cccagcgaag tggattacaa cgttggctac acctcacttg cttccggcga tgcaaccttc
     8341 accgccgtga actggacgcc gctgcatgac aacatgtacg aagctgccgg tggcgataag
     8401 aaattttatt gtgaaggggt attcgttaac ggcgcggcac agggttacct gatcgataag
     8461 aaaaccgccg accagtacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
     8521 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggctgtaa cccaggctgg
     8581 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
     8641 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
     8701 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
     8761 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaacgccgat
     8821 accaaactgc cgaatggtgc gaattatggc ttcccggtca gcaccatgca tatcgttgcc
     8881 aacaaagcct gggccgagaa aaacccggca gcagcgaaac tttttgccat tatgcagttg
     8941 ccagtggcag atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
     9001 gatattcagg gccatgttga tggttggatc aaagcccacc agcagcagtt cgatggctgg
     9061 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag tcgtttttgc
     9121 ctcatccgcc attctgtgca caatgcctga tgcgacgctg gcgcgtctta tcatgcctac
     9181 aaaatacact cattccgtat ggcggataag gcgttttcgc cgcatccgcc gttctgtgca
     9241 caatgcctga tgcgacgctg gcgcgtctta tcatgcctac aaaccacatc gcacatttca
     9301 acaagctatc tttcgccccc attcatcaac atccgctatt attgatttcc agcttaatca
     9361 tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg gcgctgatcg
     9421 tgctgatgtc tatcgccacc ggtcaggcgg tagccagtaa ctattacgcc cagccattgc
     9481 tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt attgttaccg
     9541 ccgcgcagtt gggctatgca gcaggtctgc tgtttcttgt tcccctcggt gatatgtttg
     9601 aacgccgccg cctgattgtc tcgatgacct tactggcggc aggcggcatg ttaatcactg
     9661 ccagcagtca gtcgctggcg atgatgatcc tcggtacggc attaaccggt ttattctcag
     9721 tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat aaacgcggca
     9781 aagtggttgg caccattatg agcggtctgc tgttggggat cttgctggca cgaaccgttg
     9841 ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgcc tcggtgttaa
     9901 tggcactgat ggcgctagca ttatggcgtg gtctgccaca aatgaaatca gaaacccacc
     9961 tcaactaccc acagttgtta ggttccgttt tcagcatgtt tatcagcgat aagatcctcc
    10021 gcacccgcgc attgctgggc tgcctgacct ttgccaactt cagcattctc tggacctcaa
    10081 tggccttttt gcttgccgct ccacctttta actacagcga tggtgtcatt ggtctgtttg
    10141 gacttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt gccgataagg
    10201 gtaaatcgca ccacaccaca actttcggtc tgctgctgct attactttca tggctggcga
    10261 tctggtttgg tcacacttcc gtactggcgc tgattatcgg catcctggtg ctggatctca
    10321 ccgtacaggg cgtgcatatc actaaccaga cggtaattta tcgaatacac cctgatgcgc
    10381 gtaatcgcct gaccgcaggt tacatgacca gctactttat tggcggtgcc gccggttcgc
    10441 taatttcagc ctcagcctgg caacatggcg gttgggctgg cgtttgtctg gctggcgcga
    10501 cgattgccct agttaactta ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa
    10561 attaagcaat cagcaaccgt tttcgtggct ttacacttat aagggtgtta agaagcccat
    10621 cagtctgata aggttaagat attcattcag tctatttata atattaacaa tcgttaagcg
    10681 tacactctat ggaaagccct actccacagc ctgctcctgg ttcggcgacc ttcatggaag
    10741 gatgcaaaga cagtttaccg attgttatta gttatattcc ggtggccttt gcgttcggtc
    10801 tgaatgcgac ccgtctggga ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt
    10861 atgcaggcgc gagccagttc gtcattaccg cgatgctggc agccgggagt agtttgtggg
    10921 ttgctgcact gaccgtcatg gcaatggatg ttcgccatgt gttgtatggc ccgtcactgc
    10981 gtagccgtat tattcagcgt ctgcaaaaat cgaaaaccgc cctgtgggcg tttggcctga
    11041 cggatgaggt ttttgccgcc gcaacagcaa aactggtacg caataatcgc cgctggagcg
    11101 agaactggat gatcggcatt gccttcagtt catggtcatc gtgggtattt ggtacggtaa
    11161 taggggcatt ctccggcagc ggcttgctgc aaggttatcc cgccgttgaa gcagcattag
    11221 gttttatgct tccggcactc tttatgagtt tcctgctcgc ctctttccag cgcaaacaat
    11281 ctctttgcgt taccgcagcg ttagttggtg cccttgcagg cgtaacgcta ttttctattc
    11341 ccgtcgccat tctggcaggc attgtctgtg gctgcctcac tgcgttaatc caggcattct
    11401 ggcaaggagc gcccgatgag ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg
    11461 aattattgct tccgctattt gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa
    11521 cgtggcgcgg taggtatttt gctcgacacc attggcatcg cctcgatatg cgctctgctg
    11581 gttgtctcta ccgcaccaga agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc
    11641 ggcttcgcgg tactgggtgc cagtttctat aaaacacgca gcattatcat cccaacactg
    11701 cttagtgcgc tggcctatgg gctcgcctgg aaagtgatgg cgattatata acccacaaga
    11761 atcatttttc taaaacaata catttacttt atttgtcact gtcgttacta tatcggctga
    11821 aattaatgag gtcataccca aatggatagt tcgtttacgc ccattgaaca aatgctaaaa
    11881 tttcgcgcca gccgccacga agattttcct tatcaggaga tccttctgac tcgtctttgc
    11941 atgcacatgc aaagcaagct gctggagaac cgcaataaaa tgctgaaggc tcaggggatt
    12001 aacgagacgt tgtttatggc gttgattacg ctggagtctc aggaaaacca cagtattcag
    12061 ccttctgaat taagttgtgc tcttggatca tcccgtacca acgcgacgcg tattgccgat
    12121 gaactggaaa aacgcggttg gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg
    12181 catctgcaat taacggaaaa aggtcacgag tttttgcgcg aggttttacc accgcagcat
    12241 aactgcctgc atcaactctg gtccgcgctc agcacaacag aaaaagatca gctcgagcaa
    12301 atcacccgca aattgctctc ccgtctcgac cagatggaac aagacggtgt ggttctcgaa
    12361 gcgatgagct aacgcgtcat ctcgctcaaa aatccagatt tataaaagaa aaaatgaccg
    12421 gccagcatcg caacatgctg gcctttttgg caagcaggtc ggctcagccg atgagttaag
    12481 aagatcgtgg agaacaatat gagcgcaaat gcggagactc aaaccccgca gcaaccggta
    12541 aagaagagcg gcaaacgtaa gcgtctgctc ctccttctca ccttgctctt tataattatt
    12601 gccgtagcga tagggattta ttgttttttg gtactgcgtc atttcgaaga aaccgatgac
    12661 gcatacgtgg cagggaatca gattcaaatt atgtctcagg tgtctggcag cgtgacgaaa
    12721 gtctgggccg ataacaccga ttttgtaaaa gaaggcgacg tgctggtcac tctcgacccg
    12781 acagatgctc gccaggcgtt tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa
    12841 acccaccagc tgatgattaa cagcaaacag ttgcaggcga atattgaggt gcagaaaatc
    12901 gccctcgcaa aagcacaaag cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg
    12961 attggtcgcg aagagctgca acacgcccgc gacgccgtca ccagtgccca ggcgcaactg
    13021 gacgtcgcta ttcaacaata caatgccaat caggcgatga ttctggggac taaactggaa
    13081 gatcagcctg ccgtgcaaca ggctgccacc gaagtacgta acgcctggct ggcgctggag
    13141 cgtacccgta ttgtcagtcc gatgaccggt tatgtctccc gccgcgcggt acagcctggg
    13201 gcgcaaatta gcccaacgac gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg
    13261 gatgccaact ttaaagagac gcagattgcc aatatgcgta tcggtcagcc ggtcactatc
    13321 accacggata tttacggcga tgatgtgaaa tacaccggta aagtggttgg tctggatatg
    13381 ggcacaggta gcgcattctc actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa
    13441 gtcgttcagc gtctacctgt gcgtatcgaa ctggaccaga aacagctgga gcaatacccg
    13501 ctgcgcattg gtttgtccac actggtgagc gtcaatacca ctaaccgtga cggtcaggta
    13561 ctggcaaata aagtacgttc aacaccggtc gcggtaagta ccgcgcgtga aatcagcctg
    13621 gcacctgtca ataaactgat cgacgatatc gtaaaagcta acgctggcta atccagaggt
    13681 gcgtgtgatg caacagcaaa aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc
    13741 gctgtcactg gcgacattca tgcaggtgct ggactccacc attgctaacg tggcgatccc
    13801 cactatcgcc gggaatctgg gctcatcgct cagccaggga acgtgggtaa tcacttcttt
    13861 cggggtggcg aatgccatct cgatcccgct taccggctgg ctggcaaagc gcgtcgggga
    13921 agtgaaactg ttcctttggt ccaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt
    13981 ctccagcagc ctgaatatgc tgatcttctt ccgcgtgatt caggggattg tcgccgggcc
    14041 gttgatcccg ctttcgcaaa gtctattgct gaataactac ccgccagcca aacgctcgat
    14101 cgcgctggcg ttgtggtcga tgacggtgat tgtcgcgcca atttgcggcc cgatcctcgg
    14161 cggttatatc agcgataatt accactgggg ctggatattc ttcatcaacg tgccgattgg
    14221 cgtggcggtg gtgttgatga cactgcaaac tctgcgcgga cgtgaaaccc gcaccgaacg
    14281 gcggcggatt gatgccgtgg ggctggcact gctggttatt ggtatcggca gcctgcagat
    14341 tatgctcgac cgcggtaaag agctggactg gttttcatca caggaaatta tcatccttac
    14401 cgtggtggcg gtggtggcta tctgcttcct gattgtctgg gagctgaccg acgataaccc
    14461 gatagtcgat ctgtcgttgt ttaagtcgcg caacttcacc atcggctgct tgtgtatcag
    14521 cctcgcgtat atgctctact tcggcgctat tgttctgctg ccgcagttgt tgcaggaggt
    14581 ctacggttac acggcgacct gggcaggttt ggcctctgcg ccggtaggga ttattccggt
    14641 gatcctgtcg ccgattatcg gccgcttcgc gcataaactg gatatgcggc ggctggtaac
    14701 cttcagcttt attatgtatg ccgtctgctt ctactggcgt gcctatacct ttgaaccagg
    14761 tatggatttt ggcgcgtcgg cctggccgca gtttatccag gggtttgcgg tggcctgctt
    14821 ctttatgccg ctgaccacca ttacgctgtc tggtttgcca ccggaacgac tggcggcggc
    14881 atcgagcctc tctaacttta cgcgaacgct ggcggggtct atcggcacgt cgataaccac
    14941 gaccatgtgg accaaccgcg agtcgatgca ccatgcgcag ttgactgagt cggtaaaccc
    15001 gttcaacccg aatgcccagg cgatgtacag tcaactggaa gggcttggga tgacgcaaca
    15061 gcaggcatca ggctggattg cccagcagat caccaatcag gggctgatta tttccgccaa
    15121 tgagatcttc tggatgtcag ccgggatatt cctcgtactg ctggggctgg tgtggtttgc
    15181 taaaccgcca tttggcgcag gtggcggtgg cggcggtgcg cactaagtac aactaagcca
    15241 gttcatttga actggctttt ttcaattaat tgtgaagata gtttactgac tagatgtgca
    15301 gttcctgcaa cttctctttc ggcagtgcca gttcttcgtt gctgttgatg cgtacgtcac
    15361 gttccagaat gctacgcgca atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc
    15421 cacactggta gacgttcagt tccgggatct gattctgatc ctgcactttc agcacgtctt
    15481 ccattgccgc tttccaggca tcagcaacac gctgctcatc tggcgtacca atcagactca
    15541 tataaaaacc ggtgcggcag cccattggcg agatatcgat aatctctaca ccattaccgt
    15601 taagatggtt acgcataaaa ccagcaaaca ggtgctccag ggtatggatc cctctttctg
    15661 gcatcacttc tttgttcggc acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat
    15721 gcggggtgtt cattgttttc gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga
    15781 ctgtgaagct atctaacaac ggcatttagc cacctccggt aattttttta aaaattttct
    15841 gaactctttc ttcccaggcg agtctgagta tatgaaagac gcgcatttgt tatcatcatc
    15901 cctgaattca gagatgaaat tttggccact cacgagtggc ctttttcttt tctgtcaggt
    15961 gtgtttttcc agccacaccg caaacggttc ggtatcagcg gtttccattt cctgctgacg
    16021 gcgttcagat gcctcgcgct cggctacaaa atcctcttcg cgcagaattt ccagcggctc
    16081 ttcacgcagc agattacggt aggcttctgc aaacgctttg cctgttccgc caataccagt
    16141 atcaatcata gaccttaaga tacgggcaga gaaagtcaga tcgggattat cgaagcaggc
    16201 aaccagttca tcacacactt tctgatacgc gtcgccgccg ttaatgctat ccagcgtttg
    16261 cgcgacgcgt ttcagatcgc ggaacagatc tttacccacc tgcagtaacg ggaactgcgc
    16321 ggtttcgcag ccgataccca gcgtcaggcc cggtttgcga ccttcaagga tcacccggtt
    16381 ccagttaacg cgcgtacagg caagttcgct actgctcatt tccggtgcat cagccagcgc
    16441 acaccagacc ataaacaggt cgaggaatcg cacctgctgt tcatctacac caatcggcga
    16501 gaacgggttg atgtccagcg aacgcacttc aatatattca atgccgccac gtaacagcgc
    16561 atcagaaggt gactcgccgc tgcgggtaac gcgttttgga cgaatcggcg catacagttc
    16621 gttttcaatc tgcagcacgt tgctattgat ttgcagcctc ttaccgtctt tctcaatacc
    16681 aatcttcgcg tactcttccg atggcgtttt gattgcctgt ttaaggcccg ctacgtactc
    16741 gtaaagatcg ttgaaggtaa taccaagatt gctttgcgat ttattggtat agccgagatc
    16801 gctcaaacga agagaggtcg catacggcag gtaatacata ccgcactcgg ttttctcaaa
    16861 cggcagcgac gttggttttc cttgcaggaa agaagaacaa atcgccggag atgcaccaaa
    16921 cagataagga atgacccaac cgaaacgata gtaattgcgg ataacgcgga aatagcccgc
    16981 agaaattttc tctttggcat cagcgcccga gatatcaccg cacttcgctt gccagaatgc
    17041 cattggcaaa gagaaattgt agtgcacgcc ggaaatggtt tgcatcagcg cgccgtagcg
    17101 atttttcagc ccttcacgat acagcgtttt aaagcgtccg gtgttagaag tgccgtactg
    17161 tgccagttcg atgtcctgac cttctgcgat gtagcatggc atacttaacg gccacatccg
    17221 ctcatcgccc atattgcgcg ccgtataacg atgcagatcg cgcataaagg tcagcatatg
    17281 ttcaatatca ccatccactg gtgtaatgaa ttccagcaat gcttccgcaa aatcggtagt
    17341 aatccatttg tgcgtcagtg cggaacctaa tgcttcagga tgacctgttg ttgccagtgt
    17401 gccatcagca ttaacacgca aagtttcgcg ctccagccca cgctgtatcc cctttaacgc
    17461 ctgaggatgt ttttccagcc aggccagcgc ctgtgatacg tccgggatca aattgacctc
    17521 ccgcctgtca aaatcgtttt aattagcata actgtaatgg tgaccatatg tgcaggccta
    17581 caattagtgc caccacatca tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa
    17641 cgctttacca aggcacaaaa aaaagattac cggtccccac gagatgcgca tccatcccgc
    17701 taacagacac agtaaatcgc caaccacggg catccagctt aataatagcg tgactgcacc
    17761 atagcgtttc agccagccga tagctttctc ttgccagcgc gatgttttac gcaatggaaa
    17821 gaaacgccca aggataacgt tagttaaccc tccaaggcta ttacccattg ttgctgttaa
    17881 aactaaaacc cagggatgac tgatcccgga aagcaacatt gccaccagca cgacttcgga
    17941 gttgccgggt aatagtgtag cgctgagaaa actactggca aacaacgaga aaagcgataa
    18001 cgcttcactc acagcaagcg aacgtccaca gcgtccatac ctgcggcacg ggccgcctga
    18061 ataccgaaat cggcatcttc aaagaccaca cactgcgtcg gttgcacgcc catacgctgc
    18121 gcgcacaaca aaaatgtgtc tggcgcgggt ttatggtgtt tgacgtgatc ggcagcgacg
    18181 acggcgtcaa aataatggcg taatcccagg tgcgccagca atgcctcagc gatggcgctt
    18241 tcactccccg ttcctacagc cattgggcga cgaccatgcc aacttttcac cacatcaaca
    18301 agaggaagcg gttcgacgct atccagcagc atacttctta ctgcttctgt tttttcacgc
    18361 gctaacgcat gcgggtcgag atcggcctga ttcagctcaa taattgcctg agcaatacgc
    18421 caggtgggcg atccattaag cgcaatcatc gcctgaatat cgtactgaag accgtagtgc
    18481 cctaatactt cgcgccacgc tttacggtgc gtaggctccg tatccaggat tgtgccatcc
    18541 atatcaaaaa ttaaacctgc ataacgctcg tacatggtct tctcgcaagt cgaaaaatat
    18601 gacgctactt tagcgtaatt gcttgatttt gtcgctgatg gcgaaggaga agaaaattca
    18661 ggaaatggat aaaggagtta tttagaaatg agatattttt gaaggaaatt ttttggagaa
    18721 gatggtgcat ccgggaggat gactcactga cgttcgccct tcgggccgtt gctggcgcaa
    18781 cgttatcctt cctggtgcct gcggttaata ccgctatctc ttcgatacct tcattgctga
    18841 aggttacgga gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
    18901 ccgagtactc tatccagctg agctacggat gcatcgggaa aacttattct actgctgata
    18961 ttgataccgc tactaaagcc atatcaatta agagatggtg catccgggag gattactcgc
    19021 cttgcggctc gcccttcggg ccgttgctaa agcaacgtta tcatccctgg tgcctgcggt
    19081 taattccgct acctcttcga taccttcatt gctgaagatt tcggagaata tggtgcatcc
    19141 gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta
    19201 cggatgcatc gggaaaattt attttactgc tgatattgat accgctacta aagccatatc
    19261 aagtaagaga tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag
    19321 tactctatcc agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga
    19381 tgcagctttt gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc
    19441 accacacgcc tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg
    19501 cgcacatatt actttctgag acttataagt caaacaattt ttcccacact tttatcgttt
    19561 gcacacttca cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa
    19621 aacggaggtc tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg
    19681 gagtaaagcg aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa
    19741 agatgagacg cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg
    19801 gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg
    19861 gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc
    19921 aactcgacga gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc
    19981 tctgtatccc ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta
    20041 catgtaagca gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact
    20101 tcaacctgac tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg
    20161 cattgcctga cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa
    20221 cgctgcaggt aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc
    20281 acccttgccg cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc
    20341 tgtgacgtcc ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag
    20401 cacgataatt gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt
    20461 cggctcaaca ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt
    20521 actggaaaga tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt
    20581 ttctaactga cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc
    20641 gcctttcagc agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat
    20701 agcgccttct ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga
    20761 gatgatgcgg aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc
    20821 ggtggagaaa tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag
    20881 cgccatcgca cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa
    20941 attgcgacga atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat
    21001 cgcttcgttg tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg
    21061 cgatacatga gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg
    21121 attcagacga atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac
    21181 tttcagagaa cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt
    21241 atcttccacc gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc
    21301 gccggattcc gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt
    21361 gatggcatca accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag
    21421 atggtcatag cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc
    21481 accaaagccg ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc
    21541 agcttcgtca actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa
    21601 gccataggtg tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt
    21661 tgccagctct tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc
    21721 ttcagtcttc agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga
    21781 gcccataacg tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag
    21841 catattgccg tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc
    21901 attggacggc attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat
    21961 tacgcgcagc gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat
    22021 caacgtgcgg aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat
    22081 acgctccaga cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc
    22141 tgcctggcgg ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc
    22201 ttcttccgga cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc
    22261 ggtgcacggg ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg
    22321 cgcacctttg ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc
    22381 ccagatttcg taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg
    22441 cagggcaaac catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt
    22501 gaaatagtcg ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt
    22561 gtaaccgacg ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt
    22621 ggtagcgcgg gaataattac gcttgtcgag cccaaggaac acatccttga actggttcat
    22681 cccggcgttg gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac
    22741 tacctgatgt cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct
    22801 cttgctcata attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc
    22861 ttaaccgccg ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt
    22921 aaaataccgt atgcgttcag tcggcaaaat ttcgccaaat atcctggata tcttccatca
    22981 gatagccacg atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca
    23041 aaggttcacc atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac
    23101 attcacgcat cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac
    23161 gaatacgtgc aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc
    23221 ggctgtcatc gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg
    23281 ccgtagcatc aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc
    23341 gcagttcttg ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc
    23401 gagcatatgc cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa
    23461 agcaaaaggg ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga
    23521 gtgtgcagcc aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc
    23581 tgttcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta
    23641 tctattatca tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct
    23701 gtcatctaca gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt
    23761 cttctcgatc tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt
    23821 accctgaccg atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt
    23881 ctcttttacg cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat
    23941 ctgaaattca gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt
    24001 ttcgctaccc accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg
    24061 aacagaggcg tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat
    24121 cacaccaatt ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag
    24181 gttacccgcc agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga
    24241 gtcgccgatt tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac
    24301 gataacgtct actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc
    24361 ggtgtccggc tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat
    24421 tgggtccagc gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc
    24481 ggcgatcacc tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac
    24541 gatacggccc atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt
    24601 agagatggtt tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc
    24661 aaattgtttc tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat
    24721 agccattttt actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc
    24781 aattatactg tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct
    24841 gcaatgcata cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc
    24901 gccgggtaat gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct
    24961 tctcttcact gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac
    25021 gagccgcttt cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat
    25081 gctgcgccag cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg
    25141 tgacaaatcc gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta
    25201 cccaaccacc ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct
    25261 gcccaacctg ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc
    25321 aaaaatttat gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt
    25381 ttacgaggag gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg
    25441 caaatagtta ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg
    25501 cgtaatgggt gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt
    25561 actggtaacc ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg
    25621 gctgctgtgg cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc
    25681 catttggcaa gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc
    25741 agccgtgcgc tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt
    25801 tgatatgccc gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact
    25861 gtccgtagcc catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt
    25921 cgcgcgccat caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt
    25981 agttaaatga cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc
    26041 agcgggtttc aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc
    26101 acgcgcgatt caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt
    26161 ccggcgtaat aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt
    26221 tcaccgatgt ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg
    26281 cctgggagag aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt
    26341 cgatgaactg ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt
    26401 gctgaggctc aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag
    26461 gttttggctt gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac
    26521 gacgcttgaa catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca
    26581 tcacgccccg cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact
    26641 cattcc
//
LOCUS       DAZOYS010000036        26208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOYS010000036 DAZOYS010000000
VERSION     DAZOYS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.036"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(293..520)
                     /locus_tag="ACHUN9_001342"
     CDS             complement(293..520)
                     /locus_tag="ACHUN9_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0694784.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            712..1917
                     /gene="csdA"
                     /locus_tag="ACHUN9_001343"
     CDS             712..1917
                     /gene="csdA"
                     /locus_tag="ACHUN9_001343"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0694785.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1917..2360
                     /gene="csdE"
                     /locus_tag="ACHUN9_001344"
     CDS             1917..2360
                     /gene="csdE"
                     /locus_tag="ACHUN9_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0694786.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2411..3217)
                     /gene="tcdA"
                     /locus_tag="ACHUN9_001345"
     CDS             complement(2411..3217)
                     /gene="tcdA"
                     /locus_tag="ACHUN9_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0694787.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3294..4391)
                     /gene="mltA"
                     /locus_tag="ACHUN9_001346"
     CDS             complement(3294..4391)
                     /gene="mltA"
                     /locus_tag="ACHUN9_001346"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0694788.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4600..4676
                     /locus_tag="ACHUN9_001347"
     tRNA            4600..4676
                     /locus_tag="ACHUN9_001347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4634..4636,aa:Met,seq:cat)
     gene            complement(4751..6004)
                     /gene="amiC"
                     /locus_tag="ACHUN9_001348"
     CDS             complement(4751..6004)
                     /gene="amiC"
                     /locus_tag="ACHUN9_001348"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0694789.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6236..7567
                     /gene="argA"
                     /locus_tag="ACHUN9_001349"
     CDS             6236..7567
                     /gene="argA"
                     /locus_tag="ACHUN9_001349"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0694790.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7629..9455)
                     /gene="recD"
                     /locus_tag="ACHUN9_001350"
     CDS             complement(7629..9455)
                     /gene="recD"
                     /locus_tag="ACHUN9_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0694791.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(9455..12997)
                     /gene="recB"
                     /locus_tag="ACHUN9_001351"
     CDS             complement(9455..12997)
                     /gene="recB"
                     /locus_tag="ACHUN9_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0694792.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12990..15878)
                     /gene="ptrA"
                     /locus_tag="ACHUN9_001352"
     CDS             complement(12990..15878)
                     /gene="ptrA"
                     /locus_tag="ACHUN9_001352"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0694793.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16054..19422)
                     /gene="recC"
                     /locus_tag="ACHUN9_001353"
     CDS             complement(16054..19422)
                     /gene="recC"
                     /locus_tag="ACHUN9_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0694794.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFCRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19435..19758)
                     /locus_tag="ACHUN9_001354"
     CDS             complement(19435..19758)
                     /locus_tag="ACHUN9_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0694795.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19743..20150)
                     /locus_tag="ACHUN9_001355"
     CDS             complement(19743..20150)
                     /locus_tag="ACHUN9_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0694796.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20147..20710)
                     /locus_tag="ACHUN9_001356"
     CDS             complement(20147..20710)
                     /locus_tag="ACHUN9_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0694797.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20701..21171)
                     /locus_tag="ACHUN9_001357"
                     /pseudo
     CDS             complement(20701..21171)
                     /locus_tag="ACHUN9_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(21356..22150)
                     /gene="thyA"
                     /locus_tag="ACHUN9_001358"
     CDS             complement(21356..22150)
                     /gene="thyA"
                     /locus_tag="ACHUN9_001358"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0694798.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22157..23032)
                     /gene="lgt"
                     /locus_tag="ACHUN9_001359"
     CDS             complement(22157..23032)
                     /gene="lgt"
                     /locus_tag="ACHUN9_001359"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0694799.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23183..25429)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_001360"
     CDS             complement(23183..25429)
                     /gene="ptsP"
                     /locus_tag="ACHUN9_001360"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0694800.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25442..25972)
                     /gene="rppH"
                     /locus_tag="ACHUN9_001361"
     CDS             complement(25442..25972)
                     /gene="rppH"
                     /locus_tag="ACHUN9_001361"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0694801.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
BASE COUNT         6237 a         7258 c         6560 g         6153 t
ORIGIN      
        1 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
       61 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa
      121 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
      181 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
      241 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgcg aattactgat
      301 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
      361 ctttatacga aatcataccg gtatcgttat cagtctgtgg tttgccgtca gagacgatgg
      421 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
      481 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaagcgat
      541 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
      601 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
      661 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
      721 tttaatcccg cgcagtttcg cgcccagttt cccgcactac aggatgcggg cgtctatctc
      781 gatagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccactcg acagttttac
      841 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
      901 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
      961 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
     1021 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
     1081 gtcccctggc tgatggtcgc ccaacaaact ggagccaaag tggtgaaatt gccgcttaat
     1141 gcgcagcgac tgccggatgt cgatttgttg ccagaactga ttactccccg tagtcggatt
     1201 ctggcgttgg gtcagatgtc gaacgttact ggcggttgcc cggatctggc gcgagcgatt
     1261 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg ggcggtgcat
     1321 ttccccgcag atgttcagca actagatatc gatttctatg ctttctctgg tcacaaactg
     1381 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
     1441 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggctt cacgactcaa
     1501 tctgcgccgt ggaaactgga agcaggaacg ccaaatgtgg ctggtgtcat aggattaagc
     1561 gcggcgctgg aatggctggc agattacgat atcaaccagg cagaaagttg gagtcgcagc
     1621 ctggcgacgc tggcggaaga tgcgctggca aaacgtcctg gcttccgctc attccgctgc
     1681 caggattcca gcctgctggc ctttgatttt gctggcgttc atcacagcga tatggtgacg
     1741 ctgctggcgg agtacggtat tgccctgcgg gccgggcaac attgcgctca gccgctactg
     1801 gcagaattag gcgtaaccgg cacactgcgc gcctcttttg cgccatataa tacaaagagt
     1861 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
     1921 cgaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
     1981 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
     2041 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
     2101 aaaaccgcgt ctggctggga tataccgtga ctgaaaacgg taaaatgcac ttctttggcg
     2161 atagcgaagg gcgcattgtg cgcggcctgc tggcggtgct gttgactgcc gttgagggga
     2221 aaaccgccgc cgaattgcag gcacagtcgc cgctggcatt gtttgatgag ctgggattgc
     2281 gtgcgcaact tagcgcctca cgcagccagg ggttaaatgc gttaagcgag gcgattatcg
     2341 ctgcgacgaa gcaggtttaa gcatagaacc gggtcggata agacgttcgc gtctcatccg
     2401 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
     2461 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
     2521 ccatccgctt cggcccttct gccgtggctt tcatcgcaca cacagtaccg tctgactgcg
     2581 ggtacaccag cgcttcagta gaaaacacgc aatcgacgcc gagcttacct ttactgtttt
     2641 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
     2701 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
     2761 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
     2821 gtacactatc aatggcatca atcacgtacg aataacccac gctcatatac tgcgctacgt
     2881 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
     2941 tacgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
     3001 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
     3061 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
     3121 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
     3181 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggt
     3241 gaggttatcc ctcaccctgt cgtatccgta aaaacggtat acagaataac acatcagccg
     3301 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggttg
     3361 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
     3421 cctttgattg caccaccgac atccagcgcc accatcagac gcagttcgta ctgaccatta
     3481 aatttgccgt tattatccag caacggcact tctgccagca aggtagtacc tggcggaata
     3541 atggaacgat cagaggcaac tgacgcgcga ccaaccagcg gcaccgcact tgcccctttc
     3601 accggtgcaa aagattgcgg tttaaagaag acgaaagacg ggttctgttc cagcagctcg
     3661 cgaacctcgg cttcactgtg tgtttcgccc cagtgacgaa tcgcctgcat cgacatatct
     3721 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
     3781 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
     3841 ctaccctgca catccataat gaagttatcc atcagggagt tactgtaagc gagaatatat
     3901 ttatcactca atgcccccgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
     3961 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
     4021 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
     4081 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
     4141 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
     4201 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
     4261 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
     4321 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
     4381 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
     4441 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct
     4501 ttagtgattt gtttttgttc aaaatcatgc cagatccgtg atcggggtaa aaaaaaggtt
     4561 gcatgaaaac gcgagcggag tatagtgcgc atccacggag gcggggtgga gcagcctggt
     4621 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta
     4681 ttgaacaccc taacgggtgt ttttttgttt ctactctccc ataaaaaaag cgccattcag
     4741 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
     4801 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
     4861 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
     4921 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt ttaccgagct
     4981 tattcagcac tgctttacca aacttaaggc tgtcagcaat ggtcagcgac tgcaccatat
     5041 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
     5101 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga
     5161 gcgcaaacac cgaggaaccg ctcggctggc gactggtaaa ggcgtcggca tggatagaga
     5221 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
     5281 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
     5341 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
     5401 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
     5461 ctttacctgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
     5521 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
     5581 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
     5641 ttacgttttg ctttaattca aaaaccatcc gtacggtttg cgggtcaaat tgcccgacgc
     5701 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     5761 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
     5821 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
     5881 agacgcgcac cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata
     5941 gccacatagc gcccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
     6001 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     6061 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     6121 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
     6181 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaaggggt gtgccgtggt
     6241 aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac
     6301 ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
     6361 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
     6421 ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg aaccgctgta
     6481 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
     6541 aacattgcaa ctggatatta ctgctcgcct gtcgatgagt ctcaataaca cgccgctgca
     6601 gggcgcgcat atcaacgtcg tcagtggcaa ttttattatt gcccagccgc tgggcgtcga
     6661 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatccg
     6721 tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
     6781 cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc
     6841 tgagaagatg attggttttt gctcttcaca gggcgtcact aatgacgacg gtgatattgt
     6901 ctcagaactt ttccctaacg aagcgcaagc gcgggtagaa gcccaggaag agaaaggcga
     6961 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
     7021 gcgtcgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc
     7081 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
     7141 aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
     7201 ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagct
     7261 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
     7321 aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct
     7381 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac
     7441 gcgcagtatt cactggttcc aggaacgtgg atttaccccg gtggatattg atttactgcc
     7501 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
     7561 agggtaagcg atgaaaatcg ccggatgcgg caggcgtaac actcgtgcgt cgcatccggc
     7621 aattacgttt atccccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga
     7681 gtggcgattg ccgcacttaa tatacgctca tcggcataca gcgacagacg gcgacgcgcg
     7741 cgggtcaccg cggtgtatac cagctctcgc gttactatcg gcgtgcgttg gctcggtaaa
     7801 atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta
     7861 gtttcgtgct ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca
     7921 aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac
     7981 ccaagcgcac tgtcattacg ggcaatcatc accggtcgac cttcgtacca acgagagtgc
     8041 gaatgacgat gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca
     8101 gccacgccaa atggcccttc ccgcagggcg cacaaaagtt ggtactcatt gaacgcctga
     8161 atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc ataacccgca
     8221 agagcttcct caagcatcgc aatataatct tcgccgctct gtaaaagccg tttttcaata
     8281 tcagtaaaat cctgctgaaa aacggttttc actgccgttt tatcaccacg gttgatcgcc
     8341 gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag
     8401 agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agttcccgtc
     8461 aggcggctta gctgcccggc acgctcggcg gtaaagccgg cattggcata agcgcagata
     8521 tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc
     8581 actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt
     8641 gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa
     8701 cgctggctac ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg
     8761 cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt
     8821 aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg
     8881 gccatttgaa ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg
     8941 ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg ctgccgccac tttttgccag
     9001 ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca
     9061 acctcaatgg catgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac
     9121 atgcgattca aataaagacg atcgccacag aggatcatcg gcgtgggttc atctcccctg
     9181 ctgaccgctt gagaagccag caagcattct tcccaatttt gtagctcacc gatttcactg
     9241 acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga aagcggcaaa
     9301 cagacgtgtc cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga
     9361 tgttcatctc ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc
     9421 tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccagg gtcataccgg
     9481 caaacatctc atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt
     9541 gttgcggatg ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgac
     9601 gatcatagtc gtaatcagca atgcgatggc gcagataacg atgcagcgcc agggtataaa
     9661 gctgatattg cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt
     9721 aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt
     9781 cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca
     9841 gcggcgggca gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg
     9901 cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg
     9961 aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc
    10021 actcggtcaa caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta
    10081 cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg
    10141 tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc
    10201 tgaccacgcc tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac
    10261 cacgctgctg caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta
    10321 gtgtcctcgc attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac
    10381 cagtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa
    10441 gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga
    10501 cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc
    10561 aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt
    10621 cgacctccgc gaggtcgacg ctttctggcg cagcattaag atccaaaact gcctcaaacg
    10681 agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc
    10741 agaccagcgg atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt
    10801 tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt
    10861 gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgaagca
    10921 gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt
    10981 tttctgcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc
    11041 gccagatttg ccgataacca tcgaactctt cgactaccgc atcccacgca tgttcgtcat
    11101 tattcagcgt ttcaatatcc agcgcattca gccccatcat tgacgttgcc agcgcactgc
    11161 gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa cagccaaagc atttcctgcg
    11221 cttccagagt ttcaaaaaca ctatcgcggt tcgaaaggta aacggaaggg atttccagca
    11281 acgttaaggc atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acgctgatgt
    11341 ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc
    11401 cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgatagt
    11461 cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac
    11521 ctttaaagac aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta
    11581 tttcgcgaaa catgaacgcg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca
    11641 ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg ggcgtgaact tcactacgcg
    11701 ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc
    11761 caatcagcaa caatgcggtt tctggctgat ggtgccagat acggcgaaaa attcggtact
    11821 gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg
    11881 ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt
    11941 catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag
    12001 ccaatgcgcg ggtgatcacc agatcacgga tcgacaatgg ttctgcaagc agttgatcga
    12061 tcgcctcaaa cagtggatgt cgcggggttt cccccccggc tttcgtgcga tcttctaaga
    12121 aacgctggga gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg
    12181 cccaggcgct gatcttgtcg atccatttag cctgattgct acggttaaac ttgcgtcgat
    12241 caataccaga agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt
    12301 ttaccgtatc aatacgcgcc acaatttgcg cgtgacggga agctagcgtt tcatcatcgg
    12361 gcggcggtgc tttgataacc ggcgcttcgc cttgcagata acgattaata tcgcgcagca
    12421 acgcctgcgg ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt
    12481 agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa
    12541 tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc
    12601 aaaagccgtg aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc
    12661 actgcgcggc ttgcgcttta tcgtcgatct cttccagcag gtgttcgtac aatggattgt
    12721 cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccgc
    12781 gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acggtcagcg
    12841 ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag gcgcaaataa agcgccgcaa
    12901 tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca
    12961 agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt
    13021 gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg
    13081 gctttcccgt tttggctgcc ggaaatctgc gacaggatag ccatgccttg cggctcgact
    13141 accgcctgat ggaagaaatc ggccagtttt tgcggcgtca gcagtttaat ctgggccacg
    13201 attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat
    13261 gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt
    13321 tgcgcaaact catctggctt catcgctcgc aattttgcct ctgcggttgg gaaaaacgcc
    13381 ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg
    13441 ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa ttgttcttcg
    13501 gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct
    13561 gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg
    13621 ctgttaccgg ctttttcaaa gatgacggat tgttttttat cgaccactac atctttgttt
    13681 cgacaccact ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt
    13741 gcctgggcct cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag
    13801 gcgtcgcgat aagccagcac ctctttcaac gtaatggagg gcaaaatttt acgccgttca
    13861 tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac
    13921 gctttaccct tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc
    13981 tgatcttccg tagcggtata gctaaagtac ccctcgagca atgcctggaa cagctgcggc
    14041 agacgctggg ggtaaccatt agcattaacc ataaggccgt tgttagcgtt ggtggaaaaa
    14101 cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa
    14161 tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa
    14221 atcaggctga catcagcttt gggttcgctg gaaaaataac ggcttggcgc atacaccacg
    14281 cgcagattcg actcatcaac aatcagctct ggatggtcat atttcttctc tgacttaatc
    14341 agcgagaaat cgtcaggaat ataggggtta agctccggta aggagagcgc aatattggca
    14401 gctttttgct gccagtcagc gaaagtttgt tcgctgattt tatcgacctg atacggcgca
    14461 tcgacaaaat aagccgtttt attgtgcggc tctttcgggc tgatatacca gatacgcgca
    14521 ttctgcggcg tcatcatcgc cagacgttct tttactgctt tagcatcgta ccgatcggca
    14581 atattgactg catccagcgt atgctcaacc ggaacgcgaa tcatggtatc tgccagccat
    14641 tcgacgtaat ccatatcacg ggtgatcgac ggataacgga agtcgatatc cagcacattc
    14701 gccagttcat cgaagtattg tttatcaatg cctttttcac gtaacagatt gagatagcta
    14761 aaaattgccg ccacaacctg atcgcgatta gccaggcctt tatcggttaa agacgcagag
    14821 atcgctaata cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca
    14881 actaatccct gcttttgcag ccagtcggaa agtgtacctg ggctgcgatt accaatcaga
    14941 taggtaatca attcatccgt tttactacgg aacttcgctg agttgttatc gatgcgaaac
    15001 tcaacgcgca gaactttacg cggcattgcc gggacgtaat gaatgataat gcccttttgc
    15061 gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga
    15121 ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg gtttattgct gtaaataacc
    15181 gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc
    15241 ggattaccag gtttatcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg
    15301 tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc
    15361 gccatggtta attcagcgtt caccgcatta cgctcacgtt cggcatattt cttgtcgagc
    15421 aaaggttcag caatagcatc ggccaggcgg tctaccgcac caggcaaggc gtcgttctca
    15481 acttccagat agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc
    15541 attttgagat attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc
    15601 atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg
    15661 ggcaccacca gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc
    15721 ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg
    15781 gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaact
    15841 aacaacaata atgctttgaa ccaggtgctg cggggcattc tcggacctca taatcaactt
    15901 aattttctgt ccagattcaa cacgttaacg caaatcatct gccagaattt aatcttgtgc
    15961 tgcacgagtc agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt
    16021 aagcggatag attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg
    16081 taacaggaaa cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg
    16141 ccagagcctt tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta
    16201 agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc
    16261 gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa
    16321 tggcgcggac atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg
    16381 ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc
    16441 accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc
    16501 cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca
    16561 gccagttatc tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg
    16621 acaggcaatg actctgtcgg caagctgctg catctcctgg cactgagttt cccagaaaat
    16681 ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcgacaga acaagcgttc
    16741 ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag
    16801 cccttccaga ataaatggct cggtgtcggg gatttcgctg tcttcagtac ggaagttcac
    16861 ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc
    16921 cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt
    16981 accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg
    17041 atagttttgt ggatcaaacg gcattcgggt atggaggcaa gtaagatgcg cttttaccct
    17101 tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat
    17161 gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga
    17221 acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag
    17281 atagcggtcg tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc
    17341 aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac
    17401 cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag
    17461 aaaacgctgg ctgatacgtt cttgatccag acgctgtgcc agttcatcac gcaatagtga
    17521 cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg
    17581 ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgctgag
    17641 catatcgcga caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg
    17701 ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc
    17761 gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta
    17821 gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc
    17881 cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg
    17941 gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc
    18001 cagtaatgcc agcacgtctt ccgacacaaa gcggctgtct ggcaacgata acaggctgat
    18061 aaacgcttcc agtaccggat gcgactgccg cgcccgacgg tcggaaatgg cgtaaggcag
    18121 gtaacgatcc gcaggtgcgc taccaaacac agcccgaata aacggactgt agctgtcgat
    18181 atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc gggtcttctt ccagcatcgc
    18241 cagcaggcga tcgtgcaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt
    18301 gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat
    18361 gttcacacca gcaacggcac ggttttccag ttccagaatg tcagactgaa tgttatgcag
    18421 caggttatct ggcgtgacat cgacaaaggc gtccagctcc tggctgctct ccaggtcaga
    18481 aaggagataa atgtagtcgc gcccaagctt accccatgaa gccagcagcg ggttgccgac
    18541 atcctgttca ccatcgctgt taaagagctg cccggcattt tcgctatcac gaaatagcgg
    18601 caattcgcga tcttcaaaac tgtgtcgacg ctgacgggtc agcagtttcg ccagataagc
    18661 aggatcttta atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc
    18721 aatatgttta cccagcgcct gtagcgcctg gagataaaca ggcggtaacg cggaaatacc
    18781 gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc
    18841 gataaagcgc tgatagagat tggcgcggtg ccagcgcggt tgcccgagtt gatgggtata
    18901 ttccaccagc gccttccaca acggggcttg ccaggcctgt gcttctccca gcccttcaac
    18961 caaatgtcct gtttcccact gtgccagcca gtccggacga tagaccagat actggtcaaa
    19021 caggtccgcc gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag
    19081 ataatgccgc aacagggtaa agtcttcgcg ctccagcaat tgcggcagca gagtcatcag
    19141 tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg
    19201 gacgaacata tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatact
    19261 aaacttttgc gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat
    19321 catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc
    19381 ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg
    19441 acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt
    19501 aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc
    19561 aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta
    19621 ctggtttctg ctggcgagac tgttcattaa tgttcgctgg atacccgata acgcagtgac
    19681 aatcatcacc atcagcacca tcgcaaccat tacctccggc aggctaaagc cttgttgatt
    19741 cttcagggaa gctgacataa cgccctctct ttcagcggac aaaaatcgct ccagccgcgt
    19801 ggcgaaaaaa caatgtttcc atcaatgact tcgcctgttc gccacaacga aacgccttca
    19861 taaccggcaa ccaataaggc ttcattatct gccagtaaac gcaaacaaac ccgggcatcg
    19921 gttccagcgt actgcgagca ctgaactgcg ggctgcgtct gccaggagtg cattttcccc
    19981 cacgccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc tatgctcacg
    20041 cgagaggcaa aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc
    20101 agcagcatca ggaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc
    20161 ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca
    20221 tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt
    20281 cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg
    20341 caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcagactctt
    20401 ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt
    20461 caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc
    20521 tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct
    20581 tacgggtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgca gcccccagca
    20641 acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt
    20701 ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac
    20761 caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt
    20821 gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttagactt cgggccagcg
    20881 tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac
    20941 aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt
    21001 ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccatagccg
    21061 ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca tgaccaaaat
    21121 cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt
    21181 atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga
    21241 ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaacg
    21301 gagggtaaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    21361 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    21421 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    21481 gttcgcggct taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtatcgc
    21541 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    21601 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    21661 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    21721 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgggaat
    21781 ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatctg
    21841 gcgttggcca ggcgcgccac tgtttaccat acactggtcc gaggtcgccg ttttcatcgg
    21901 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct
    21961 gcagaaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgttta gttgtcacca
    22021 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    22081 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    22141 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    22201 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    22261 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    22321 acgcgccgta gccaatcagg aacaagccag agacagctcc cattgggcgc ggtttacgaa
    22381 tatacaggtt gaggataata aacagcacca caccttccag cagcagctcg taaagctgtg
    22441 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    22501 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    22561 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    22621 caaacggaat gagtggtgcg ataaaatcag aaacctggaa gaaggaacgt ttagtacggc
    22681 gggcaaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    22741 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    22801 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    22861 caacttcatt tttggtccag ccgctgcccg gacgattcgc ccgtcgtgtt gccagccaca
    22921 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    22981 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    23041 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    23101 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    23161 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    23221 gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac
    23281 gctgcgcaag attttctgct tcggcataat caatgcgccg cagcaggtat tttacccgcg
    23341 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    23401 acatgggatc gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt
    23461 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg
    23521 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    23581 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    23641 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    23701 gtccggcacg ttcaatcagg cggcgtgctt catcaacttc atcgaggctt gtgaccatcg
    23761 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    23821 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    23881 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    23941 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    24001 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    24061 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    24121 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    24181 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    24241 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    24301 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    24361 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    24421 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    24481 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    24541 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    24601 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    24661 cgcgacgcag ccgggtgtcc gaaagtaggt gcgagtaaag atcgaaaata gccgccgttt
    24721 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    24781 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    24841 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    24901 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacaaggca gtcaactgcg
    24961 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    25021 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    25081 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    25141 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    25201 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    25261 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    25321 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    25381 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    25441 cttaacctct tttacgtcga taagcagatg tgttttgtgg ttttggcgta ttttcctgca
    25501 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    25561 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    25621 tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt
    25681 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    25741 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    25801 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    25861 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    25921 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    25981 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaagaagac ggtaaaccac
    26041 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    26101 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    26161 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcc
//
LOCUS       DAZOYS010000037        26169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOYS010000037 DAZOYS010000000
VERSION     DAZOYS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.037"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(97..1347)
                     /gene="icd"
                     /locus_tag="ACHUN9_001362"
     CDS             complement(97..1347)
                     /gene="icd"
                     /locus_tag="ACHUN9_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0694802.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1519..2172
                     /gene="rluE"
                     /locus_tag="ACHUN9_001363"
     CDS             1519..2172
                     /gene="rluE"
                     /locus_tag="ACHUN9_001363"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0694803.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2182..2643
                     /gene="nudJ"
                     /locus_tag="ACHUN9_001364"
     CDS             2182..2643
                     /gene="nudJ"
                     /locus_tag="ACHUN9_001364"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0694804.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2697..3803
                     /gene="mnmA"
                     /locus_tag="ACHUN9_001365"
                     /gene_synonym="trmU"
     CDS             2697..3803
                     /gene="mnmA"
                     /locus_tag="ACHUN9_001365"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0694805.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3839..4480
                     /gene="hflD"
                     /locus_tag="ACHUN9_001366"
     CDS             3839..4480
                     /gene="hflD"
                     /locus_tag="ACHUN9_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0694806.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4484..5854
                     /gene="purB"
                     /locus_tag="ACHUN9_001367"
     CDS             4484..5854
                     /gene="purB"
                     /locus_tag="ACHUN9_001367"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0694807.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6022..6693
                     /gene="phoP"
                     /locus_tag="ACHUN9_001368"
     CDS             6022..6693
                     /gene="phoP"
                     /locus_tag="ACHUN9_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0694808.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6693..8153
                     /gene="phoQ"
                     /locus_tag="ACHUN9_001369"
     CDS             6693..8153
                     /gene="phoQ"
                     /locus_tag="ACHUN9_001369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0694809.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8229..9350
                     /gene="roxA"
                     /locus_tag="ACHUN9_001370"
                     /gene_synonym="ycfD"
     CDS             8229..9350
                     /gene="roxA"
                     /locus_tag="ACHUN9_001370"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0694810.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9399..10625)
                     /gene="pepT"
                     /locus_tag="ACHUN9_001371"
     CDS             complement(9399..10625)
                     /gene="pepT"
                     /locus_tag="ACHUN9_001371"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0694811.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRVAELTAQRK"
     gene            10875..12011
                     /gene="potA"
                     /locus_tag="ACHUN9_001372"
     CDS             10875..12011
                     /gene="potA"
                     /locus_tag="ACHUN9_001372"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0694812.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11995..12852
                     /gene="potB"
                     /locus_tag="ACHUN9_001373"
     CDS             11995..12852
                     /gene="potB"
                     /locus_tag="ACHUN9_001373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0694813.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12849..13643
                     /gene="potC"
                     /locus_tag="ACHUN9_001374"
     CDS             12849..13643
                     /gene="potC"
                     /locus_tag="ACHUN9_001374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0694814.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13640..14686
                     /gene="potD"
                     /locus_tag="ACHUN9_001375"
     CDS             13640..14686
                     /gene="potD"
                     /locus_tag="ACHUN9_001375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0694815.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14842..15663)
                     /gene="cobB"
                     /locus_tag="ACHUN9_001376"
     CDS             complement(14842..15663)
                     /gene="cobB"
                     /locus_tag="ACHUN9_001376"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0694816.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15679..16590)
                     /gene="nagK"
                     /locus_tag="ACHUN9_001377"
     CDS             complement(15679..16590)
                     /gene="nagK"
                     /locus_tag="ACHUN9_001377"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0694817.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16619..17863)
                     /gene="lolE"
                     /locus_tag="ACHUN9_001378"
     CDS             complement(16619..17863)
                     /gene="lolE"
                     /locus_tag="ACHUN9_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0694818.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17863..18564)
                     /gene="lolD"
                     /locus_tag="ACHUN9_001379"
     CDS             complement(17863..18564)
                     /gene="lolD"
                     /locus_tag="ACHUN9_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0694819.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18557..19756)
                     /gene="lolC"
                     /locus_tag="ACHUN9_001380"
     CDS             complement(18557..19756)
                     /gene="lolC"
                     /locus_tag="ACHUN9_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0694820.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20018..21091
                     /locus_tag="ACHUN9_001381"
     CDS             20018..21091
                     /locus_tag="ACHUN9_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0694821.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21219..24665
                     /gene="mfd"
                     /locus_tag="ACHUN9_001382"
     CDS             21219..24665
                     /gene="mfd"
                     /locus_tag="ACHUN9_001382"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0694822.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24809..25765
                     /gene="ldtC"
                     /locus_tag="ACHUN9_001383"
                     /pseudo
     CDS             24809..25765
                     /gene="ldtC"
                     /locus_tag="ACHUN9_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25848..26105)
                     /gene="bhsA"
                     /locus_tag="ACHUN9_001384"
                     /gene_synonym="ycfR"
     CDS             complement(25848..26105)
                     /gene="bhsA"
                     /locus_tag="ACHUN9_001384"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0694823.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6349 a         6820 c         6659 g         6341 t
ORIGIN      
        1 tcatataaat atgctcgata tgctattaat aatatccaca taaataaaac aacgggcgtg
       61 ttatacgccc gtttcaatat ttaactcatg gagagattac atgttttcga tgatcgcttc
      121 accaaactct gaacatttca gcagcttagc gccttccatc agacgttcaa agtcataagt
      181 tacggttttc gcgttgatag caccttccat acctttaaca attaaatcag ccgcttcagt
      241 ccaccccata tggcgcagca tcatctcagc ggagaggatg atagagcctg gatttacttt
      301 gtcctgaccg gcatatttcg gcgcagtacc gtgggtggct tcaaacaggg cgcattcgtc
      361 accgatgttt gcgccagggg cgataccgat accgccaacc tgcgctgcca gggcgtcaga
      421 aatgtagtca ccgttcaggt tcatacaggc gataacatca tattcagccg gacgcagcag
      481 gatctgttgc aggaatgcat cagcaatcac gtctttaatg acgatctctt tgccggtgtt
      541 cgggttttta actttcagcc acgggccgcc gtcgatcagt tcaccgccaa actcttcacg
      601 cgccagctgg tagccccagt ctttaaacgc gccttcggtg aacttcatga tgttgccttt
      661 gtgcaccaga gtcacagagt cacgatcgtt agcaattgcg tattcgatcg ctgcacgaac
      721 cagacgtttg gtgccttctt ccgaacacgg cttaataccg ataccacaat gttccgggaa
      781 gcgaattttc ttcaccccca tctcttcacg caggaattta atcactttct cggcgtcggc
      841 agagtctgct ttccattcga tacccgcata aatgtcttcc gagttttcac ggaagataac
      901 catatcggtc agttcagggt gtttaaccgg gcttggagtg ccctgatagt aacgtaccgg
      961 acgcaggcag atgtagagat ccagttcctg gcgcagggca acgttcagag agcgaatacc
     1021 gccaccaaca ggagtggtca gcggaccttt aatggcaacg cgatattcac gaatcagatc
     1081 aagggtttca gcaggcagcc agacatcctg accataaacc tgtgtggatt tttcaccggt
     1141 gtaaatttcc atccaggaga ttttacgctc gcctttatag gctttctcga ctgcagcgtc
     1201 gaccactttc agcatggctg gggttacatc tacaccgatt ccatcacctt caatgtaagg
     1261 gataatcgga ttttcaggaa cgttgagttt gccgttttgc agggtgatct tcttgccttg
     1321 tgccggaaca actactttac tttccattca cctctccttc gagcgctact ggtttgctcg
     1381 tctgccacca cgttgcatat gcgtttgcgt cctgcgatac ggatgcttta gagcaatttt
     1441 ttgttaatga tttgtaattg gcttgtcaat gctaccctat tgtttgccgt catgaaagat
     1501 gcgcgttatt aggctataat gcggcaattc ataatctctg aaaataccat gcaaaaaact
     1561 tcttttagaa atcaccaggt taagcgattc agctcgcaac gttctactag gcgtaaacct
     1621 gaaaaccagc ccacgcgtgt gatcctgttc aataaaccct acgatgttct tccgcagttc
     1681 accgatgaag ccggacgcaa aacattaaaa gaattcatcc cggttcaggg cgtttatgca
     1741 gcaggtcgtc ttgaccgcga tagcgaaggg ttactggtgc tgaccaataa cggcgcgttg
     1801 caggcgcgtt taacccagcc gggcaaacgc accgggaaaa tctattatgt gcaggtggaa
     1861 ggtattccca cacaagacgc acttgaagcc ttgcgcaatg gcgtaacctt aaatgatggc
     1921 cctaccctgc ccgccggcgc ggagctggtt gacgaaccag cgtggttatg gccgcggaat
     1981 ccaccaattc gtgaacgcaa aagtattccc accagctggc tgaagatcac cttatatgaa
     2041 ggacgtaatc gccaggtgcg ccgcatgacc gcccatgttg gtttccccac gctgcgactg
     2101 attcgttatg cgatgggtga ttacactttg gataaccttg ccaatggcga atggcgagaa
     2161 gcaacagaat aaggatgcac aatgtttaaa ccgcacgtta ccgttgcttg cgtagtgcac
     2221 gcagagggta aatttttagt cgttgaagag acgattaatg gtaaagcgtt atggaaccaa
     2281 cctgccgggc atctggaagc cgatgaaacc ttagtggaag ctgccgcccg tgagctatgg
     2341 gaagaaaccg gcatcagcgc gcaaccgcaa cactttattc gtatgcatca gtggattgcg
     2401 ccagataaaa cgccgttttt gcgtttcctc tttgccattg agcttgagca aatatgcccg
     2461 acccagcctc atgacagcga tatcgactgc tgtcgttggg tcagcgccga agaaatttta
     2521 aaggcgtcaa atcttcgttc gccgctggtg gcggaaagta tccgttgtta tcaaagtggg
     2581 caacgctatc cgctggagat gattggcgat tttaactggc cttttacaaa gggtgtcatc
     2641 taaaaggctg cgtgatagaa tacgccgcct tgaagttcaa tgtcgtgagt gatccaatgt
     2701 ctgaaaccgc aaaaaaagta atcgtcggca tgtccggcgg tgtcgattcc tccgtttctg
     2761 cctggctgtt gcaacaacag ggatatcagg ttgaaggcct gtttatgaag aactgggaag
     2821 aagacgacgg tgaggaatat tgcacagcgg cagcggatct ggctgatgcc caggctgtct
     2881 gcgacaagct cggcattgaa ctgcacaccg ttaactttgc tgccgagtac tgggacaatg
     2941 tcttcgaact gttccttgcc gaatataaag ccggtcgcac gccgaatccg gatattctgt
     3001 gcaacaaaga gatcaaattt aaagctttcc tcgaatttgc cgccgaagat ttaggtgccg
     3061 attatatcgc taccggtcat tacgtacgtc gggccgatgt cgatggcaag agccgcctgc
     3121 tgcgtggtct ggacagcaat aaagaccaga gctacttcct ttatacgctc agccatgagc
     3181 agattgcgca aagcctgttc ccggtcggcg aactggaaaa accgcaggtg cgtaagattg
     3241 ctgaagatct tggtctggtc accgcgaaga aaaaagactc taccggcatt tgcttcattg
     3301 gcgaacgtaa attccgcgag ttcctgggcc gttatctccc ggggcaaccg ggcaaaatca
     3361 ttaccgtcga tggcgatgaa attggtgagc accaggggct gatgtatcac actctcggtc
     3421 agcgtaaagg tctgggtatc ggtggcacca aagaaggcac cgaagaacct tggtatgtgg
     3481 tggacaaaga cgtcgaaaac aacattctgg ttgtcgctca gggccatgaa cacccgcggc
     3541 tgatgtctgt cgggttgatt gcccagcagt tgcactgggt cgatcgcgaa ccattcaccg
     3601 gcactatgcg ttgcacggta aaaacccgct atcgccagac cgatatccct tgcaccgtca
     3661 aggcgctgga cgatgatcgc attgaagtga ttttcgatga accggttgcc gccgtgacgc
     3721 cgggccagtc tgccgtcttc tataacggtg aagtgtgcct cggtggcggt attattgagc
     3781 agcgtctgcc gctgccggtc tgattattat ctttacttaa aacaaggaag cagtgaacgt
     3841 ggcaaagaat tactatgaca tcaccctcgc cctggccggt atttgtcagt cggcacgcct
     3901 ggtgcaacaa ctcgctcacc aggggcattg tgatgccgat gcgctacacg tctcactcaa
     3961 cagtattatt gatatgaacc ccagctcgac gctggcggtt tttggcggta gcgaagccaa
     4021 cctgcgcgtc gggctggaaa ccctgctcgg cgtgctcaat gccagcagtc gccagggctt
     4081 aaacgccgaa ttaacccgct acacactcag cttgatggtg cttgagcgca aactctcctc
     4141 agcgaaaggc gcgctcgaca ctctgggcaa ccggatcaac ggcctgcaac gccagctcga
     4201 acacttcgat ttacagtccg aaacgctgat gagcgcgatg gctgctatct atgttgatgt
     4261 gatcagcccg cttggcccac gcattcaggt caccggttcc cctgctgtac tgcaaagccc
     4321 acaagtgcag gcgaaagttc gcgcaaccct gctggcaggc attcgcgccg ccgtgctctg
     4381 gcaccaggtc ggcggcggac gtctgcaact gatgttttct cgtaatcgcc tgaccactca
     4441 ggcaaaacaa attcttgctc atttaacccc ggagttgtga tctatggaat tatcctcact
     4501 gaccgccgtt tcccctgtcg atggacgcta cggcgataaa gtcagcgcgc tgcgcgggat
     4561 tttcagcgaa tatggtttgc tgaaattccg tgtacaagtt gaagtacgtt ggctgcaaaa
     4621 actggccgcg cacgcagcga tcaaggaagt tcctgctttt gctgccgacg caatcggtta
     4681 ccttgatgca attgtcgcca atttcagcga agaagatgcc gcacgcatca aaaccatcga
     4741 gcgtaccact aaccacgacg ttaaagcggt tgagtatttc ctgaaagaaa aagtggcgga
     4801 gatcccggaa ctgcacgcgg tttctgaatt catccacttt gcctgtactt cggaagatat
     4861 caataacctc tcccacgcat taatgctgaa aaccgcgcgt gatgaagtga tcctgccgta
     4921 ctggcgtcaa ctgattgatg gcattaaaga tctcgccgtt cagtaccgcg atatcccgct
     4981 gctgtctcgt acccacggtc agccagccac gccgtcaacc atcggtaaag agatggcaaa
     5041 cgtcgcctac cgtatggagc gccagtaccg ccagcttaac caggtggaga tcctcggcaa
     5101 aatcaacggc gcggtcggta actataacgc ccacatcgcc gcttacccgg aagttgactg
     5161 gcatcagttc agcgaagagt tcgtcacctc gctgggtatt caatggaacc cgtacactac
     5221 ccagattgaa ccgcacgact acatcgccga gctgtttgat tgcgttgcac gcttcaacac
     5281 cattctgatc gactttgatc gtgacgtctg gggttatatc gcccttaacc acttcaaaca
     5341 gaaaaccatt gctggtgaga ttggttcttc caccatgccg cataaagtta acccgatcga
     5401 cttcgagaac tccgaaggga atctgggcct ttccaacgcg gtattacagc atctggcaag
     5461 caaactgccg gtttcccgct ggcagcgtga cctgaccgac tccaccgtgc tgcgtaacct
     5521 cggcgtgggt atcggttatg cgctgattgc gtatcaatcc accctgaaag gcgtgagcaa
     5581 actggaagtg aaccgtgacc atctgctgga tgagctggat cacaactggg aagtgctggc
     5641 tgagccaatc cagacagtta tgcgtcgcta tggcatcgaa aaaccgtacg agaagctgaa
     5701 agagctgact cgtggtaagc gcgttgacgc cgaaggcatg aagcagttta tcgacggtct
     5761 ggcgttgcca gaagaagaga aagcccgcct gaaagcgatg acgccggcaa actacattgg
     5821 tcgcgccatc accatggttg atgagctgaa ataaactcgt atcagtgccg gatggcgatg
     5881 ctgtccggcc tgcttattaa gattatccgc tttttatttt ttcactttac ctcccctccc
     5941 cgctggttta tttaatgttt acccccataa ccacataatc gcgttacact attttaataa
     6001 ttaagacagg gagaaataaa aatgcgcgta ctggttgttg aagacaatgc gttgttacgt
     6061 caccacctta aagttcagat tcaggatgct ggtcatcagg tcgatgatgc agaagatgcc
     6121 aaagaagccg attattatct caatgaacat ttaccggata ttgcgattgt cgatctcgga
     6181 ttgccagacg aggacggtct gtcactgatt cgccgctggc gtagcaatga tgtttcactg
     6241 ccgattctgg tattaaccgc ccgtgaaagc tggcaggaca aagtcgaagt attaagtgct
     6301 ggtgctgatg attacgtgac taaaccattt catattgaag aggtgatggc gcgaatgcag
     6361 gcattaatgc ggcgtaatag cggtctggct tcacaggtca tttcgctacc accgtttcag
     6421 gttgatctct ctcgccgtga attatctatt aatgacgaag tgatcaaact gaccgcgttc
     6481 gaatacacca ttatggaaac gttgatacgc aataatggca aagtggtgag caaagattcg
     6541 ttaatgctcc aactctatcc ggatgcggag ctgcgggaaa gccataccat tgatgtactg
     6601 atgggacgtc tgcgcaaaaa aattcaggca caatatccgc aagaagtgat tactaccgtt
     6661 cgcggccagg gctatttgtt cgaattgcgc tgatgaaaaa attactgcgt ctttttttcc
     6721 cgctctcgct gcgggtacgt tttctgttgg caacggctgc ggtagtactg gtgctttcgc
     6781 ttgcctacgg aatggtcgcg ctgatcggtt atagcgtcag tttcgataaa actacgtttc
     6841 ggctgttacg tggcgagagc aatctgttct atacccttgc gaagtgggaa aacaataagt
     6901 tgcatgtcga gttacccgaa aatatcgaca agcaaagccc caccatgacg ctaatttatg
     6961 atgagaacgg gcagctttta tgggcgcaac gtgacgtgcc ctggctgatg aagatgatcc
     7021 agcctgactg gctgaaatcg aatggttttc atgaaattga agcggatgtt aacgatacca
     7081 gcctcttgct gagtggagat cattcgatac agcaacagtt gcaggaagtg cgggaagatg
     7141 atgacgacgc ggagatgacc cactcggtgg cagtaaacgt ctacccggca acatcgcgga
     7201 tgccaaaatt aaccattgtg gtggtggata ccattccggt ggagctaaaa agttcctata
     7261 tggtctggag ctggtttatc tatgtgctct cagccaatct gctgttagtg atcccgctgc
     7321 tgtgggtcgc cgcctggtgg agtttacgcc ccatcgaagc cctggcaaaa gaagtccgcg
     7381 aactggaaga acataaccgc gaattgctca atccagccac aacgcgagaa ctgaccagtc
     7441 tggtacgaaa cctgaaccga ttgttaaaaa gtgaacgcga acgttacgac aaataccgta
     7501 cgacgctcac cgacctgacc catagtctga aaacgccact ggcggtgctg caaagtacgc
     7561 tgcgttctct gcgtagtgaa aagatgagcg tcagtgatgc tgagccggta atgctggagc
     7621 aaatcagccg catttcacag caaattggct actacctgca tcgtgccagt atgcgcggcg
     7681 ggacattgct cagccgcgag ctgcatccgg tcgccccact gctggacaat ctcacctcag
     7741 cgctgaacaa agtgtatcaa cgcaaagggg tcaatatctc tctcgatatt tcgccagaga
     7801 tcagctttgt cggtgagcag aacgattttg tcgaggtgat gggcaacgtg ctggataatg
     7861 cctgtaaata ttgcctcgag tttgtcgaaa tttctgcaag gcaaaccgac gagcatctct
     7921 atattgtggt cgaggatgat ggccccggta ttccattaag caagcgagag gtcattttcg
     7981 accgtggtca acgggttgat actttacgcc ctgggcaagg tgtagggctg gcggtagccc
     8041 gcgaaatcac cgagcaatat gagggtaaaa tcgtcgccgg agagagcatg ctgggcggtg
     8101 cgcggatgga ggtgattttt ggtcgccagc attctgcgcc gaaagatgaa taaatatgtc
     8161 catacttcac gcattacgtt aagcatccgt tataatcggt tgcagatacc agcctgtgga
     8221 tgcttaacat ggaataccaa ctcactctta actggcccga ttttcttgaa cgtcactggc
     8281 agaaacgccc ggtggtgtta aaacgcggct ttaataattt tattgacccg atctctccag
     8341 acgagttggc gggtctggcg atggaaagcg aagttgacag tcgactggtc agtcaccagg
     8401 atggcaaatg gcaggtcagc cacggcccgt tcgaaagcta cgatcatctc ggtgaaacca
     8461 actggtcatt actggtacag gcagtgaacc actggcatga gccgaccgcc gcgctgatgc
     8521 gaccgttccg tgaactaccg gactggcgta ttgatgatct gatgatttct ttttctgtac
     8581 ctggcggcgg cgtcggcccg catctcgatc agtacgacgt gtttatcatt cagggtaccg
     8641 gacgtcgtcg ctggcgagtg ggcgaaaagc tgcaaatgaa acagcactgc ccacatccgg
     8701 atctgttaca ggtcgatccg ttcgaagcca tcatcgatga agagctggag cctggtgata
     8761 ttctttatat tccgccagga ttcccgcatg aaggctacgc gctggaaaat gcgatgaact
     8821 attccgtggg ctttcgcgcg ccaaatacgc gggaactgat tagtggattt gccgattatg
     8881 tgctgcaacg tgaactgggc ggcaactact acagcgatcc tgatgttcca cctcgcgctc
     8941 atcctgcgga tgttctgcct caagagatgg ataaactgcg tgagatgatg ctcgaattga
     9001 tcaaccagcc ggaacacttt aagcaatggt ttggcgagtt tatatcccag tcacgtcatg
     9061 aactggatat cgcgccgcca gaaccgcctt atcagccaga tgaaatctac gatgcgctga
     9121 aacaaggtga tgtgctggtg cgcctgggtg gtctgcgcgt attgcgcatt ggcgacgacg
     9181 tgtatgccaa tggtgagaag atcgattccc cgcaccgtcc ggcactggat gcactcgcca
     9241 gcaacattgc gctgactgcg gagaattttg gcgatgcgct ggaagatccg tcattcctcg
     9301 cgatgctcgc ggcgctggtc aatagcgggt attggttctt cgaagggtaa gtttggattt
     9361 aagccggatg cggcatcaca tgccgcatcc cttttcgctt acttccgttg cgccgttaac
     9421 tcggcaacac ggacgatcac ctgcaccgct ttttccatac cttccagagt cacaaactca
     9481 tgcttaccat gatagttgta accgccagtg aacaagttcg ggcacggtaa tcccataaac
     9541 gacaactgcg cgccgtcggt accaccgcgg atcggtttca gttccggttc aatatcgcag
     9601 tcacgcatcg cctgctgggc gatatcgaga atatgcggat gctcaaccac tttctctcgc
     9661 atattgtagt aactgtcttc aatcaccagt tcaatgtagc aatcaggatg taaccctttg
     9721 cccacttttt tggcgatctc catcatttta cgtttacgcg cttcaaactg tttacggtcg
     9781 aaatcacgga tgatgtagtg catatcggcc cgttcaacgg tgcctttcat gctcgccagg
     9841 tgatagaaac cttcatagcc ttctgtcatt tccgggcttt catccgccgg aacttccgca
     9901 tgaatacgtg ccgccagcga cagcgcattt accatcactc ctttcgccgt gcccggatga
     9961 acattgttac cgacaatttt gatattgacc gatgcggcgt tgaagttttc aaactccagt
    10021 tcgcctacgc caccaccgtc aacagtgtaa gcccagcggg catcgaaggc atcaacatca
    10081 aaatgtttcg cccctttgcc cacttcttca tccggggtaa aggcgacgcg aatatcacca
    10141 tgcggaatgt ttttctgttg caataccgcc agcgcggtca tgatttctgc aatacctgct
    10201 ttgtcatcgg cacctaacaa ggttttacca tcggtggtaa tcaacgtctg acccagtagc
    10261 tgatgcagca ccgggaacat aaccggtgat aaaacttcat cgccgatacc cagcgcaata
    10321 tcgccaccgc gatagttttc aacaatttgc ggattaacat ttttgccgct gcaatccggt
    10381 gaggtatcca catgagaaat aaagccaatc gccgggatat cgccagggac gttagccggt
    10441 aacgtcgcca tcaaagtgcc cttctcactt aaggtcacat tgataagccc catttcttcg
    10501 agctgctctt tcagcagatg caataacttc cattggcctt ccgtgctggg aacctgtctc
    10561 acccctgctt ttgattgggt atccagagat acgtagttca aaaatcgctc aagtagttta
    10621 tccatgtagt caccctcact ttttgtgaca acattattaa gaagcaagaa aagacaaata
    10681 ttgcgtcagg tcacttttac ccctgcaagc gggaatattt atcagcatta ccttaatgaa
    10741 tataaagcta agccagtaat tcacaacaaa gattggcgat tcaagcggtt tgccgtagaa
    10801 ttaccgccct cattaagagt caccaaggtg gttaaccaca aaccccgcat cggtaagcca
    10861 tccgttgcgt ttacatggga cagagtaaaa aattgaataa acaaccgagt tcgctttcac
    10921 cgctggtgca attggcggga attcgcaaat gctttgatgg taaagaggtc attccccagc
    10981 tggatctgac tatcaacaat ggcgagttcc tcacgctgct tggcccttct ggctgcggta
    11041 aaacaaccgt tcttcgcctg attgcaggtc tggaaactgt tgattccgga cgcatcatgc
    11101 tggataacga ggacatcacc cacgttccgg cggaaaaccg ctatgtgaac actgttttcc
    11161 aaagctacgc acttttcccc cacatgaccg tgttcgaaaa tgtggccttt gggttgcgca
    11221 tgcaaaaaac ccccgctgct gaaattacgc cccgcgtgat ggaagccctg cggatggtgc
    11281 aactggaaac cttcgctcaa cgtaaaccgc atcagctctc tggtggtcaa cagcaacgcg
    11341 tcgccattgc tcgcgcggtg gttaacaagc ctcgtctgtt gttgctggat gagtcgctct
    11401 cagcgctgga ttacaaactg cgtaagcaaa tgcagaacga gctgaaagcg ttacagcgta
    11461 agcttggtat taccttcgtc tttgtcactc acgatcagga agaagcgctc accatgtcag
    11521 acaggattgt ggtgatgcgc gagggtcgta ttgagcaaga tggtacaccg cgcgaaattt
    11581 acgaagagcc gaaaaacctg tttgttgccg gcttcattgg cgaaatcaat atgtttaacg
    11641 ccactgtcat cgaacgtctc gacgagcagc gcgtacgcgc caacgttgaa ggccgcgaat
    11701 gtaatatcta cgttaacttc gccgttgaac cggggcaaaa actgcatgtt ctgctgcgcc
    11761 cggaagactt acgtgttgaa gagattaacg acgacaacca cgctgaaggg ctgattggtt
    11821 acgttcgtga gcgtaactac aaaggcatga cgctggagtc ggttgttgaa ctggaaaatg
    11881 gcaagatggt gatggtcagc gaattcttca atgaagacga ccctgacttt gaccactctc
    11941 tcgaccaaaa aatggccatt aattgggtag aaagctggga ggtcgtactg gctgatgaag
    12001 aacacaagta agttccagaa tgtagtgatt gtcactattg tcggttggct tgtgttgttt
    12061 gtctttctgc ccaacctgat gatcattggc accagctttt tgacccgcga cgacgccagt
    12121 ttcgtcaaaa tggtctttac gctggataac tacacgcgtc tgctcgatcc gctctatttt
    12181 gaagtgctat tgcactcgct gaatatggcg ctgatcgcca ccctcgcctg cctggtgctg
    12241 ggctacccgt ttgcctggtt tctggcgaag ctgccacaca aggtgcgtcc gctgctgctg
    12301 tttctgctga ttgttccctt ctggaccaac tcactgattc gcatctacgg gctgaaaatt
    12361 ttcctcagca ccaaaggcta tctcaacgag tttttgctct ggctgggcgt tatcgacaca
    12421 ccaattcgta tcatgttcac gcccagtgcg gtgattatcg gtctggttta cattctgcta
    12481 ccgtttatgg tgatgccgct gtactccagt atcgaaaaac tggataagcc gttgcttgag
    12541 gcggcgcgcg atctcggtgc cagcaagtta cagactttta tccgtatcat tattccactg
    12601 acgatgccgg gaattattgc cggatgtctg ctggtgatgc tgccagcgat gggcctgttc
    12661 tatgtatccg acctgatggg cggtgcgaaa aacctgctga tcggtaacgt catcaaggtc
    12721 cagttcctca atattcgtga ctggccgttt ggtgcagcca ccagcattac gctgactatc
    12781 gtaatgggcc tgatgttgct ggtttactgg cgcgcttctc gtctgctgaa taaaaaggtg
    12841 gaactcgaat gatcggtcga ctgcttcgcg gcggttttat gaccgctatc tacgcgtacc
    12901 tgtatatccc aatcattatt ttgattgtga actcctttaa cagctcgcgc tttggcatca
    12961 actggcaggg ttttaccacc aaatggtata gcctgctgat gaacaacgac agcctgttac
    13021 aggcagcgca gcattcacta acaatggcgg tgttttcggc gacatttgct acgcttatcg
    13081 gttcactgac ggcagttgcg ctgtaccgtt atcgctttcg tggtaagccg ttcgttagcg
    13141 gaatgctgtt tgtggtgatg atgtcaccag atatcgtgat ggcgatttct ctgctggtgc
    13201 tgtttatgct gctgggtatt cagcttggct tctggtcgct gctgttctcg catatcacct
    13261 tctgcctgcc gtttgtggtg gtgacggtgt attcgcgcct gaaaggtttt gacgtgcgga
    13321 tgctggaagc ggcgaaagat ctcggtgcca gtgaatttac cattctgcga aaaatcattc
    13381 tgccactggc aatgccagcg gtggcggcgg gctgggtgtt aagctttacc ctgtcgatgg
    13441 acgatgtggt agtttcttcg ttcgtcaccg gaccaagtta tgaaattctg ccgttaaaaa
    13501 tttattcgat ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac
    13561 tggtgctgtc gctggtgatg gttattgcca gccagcttat tgctcgtgat aaaacgaaag
    13621 gtaacacagg ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct
    13681 ggcactgggc atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg
    13741 gaccgagtac gtgccgccag gactgcttga acagttcacc aaagaaaccg gtattaaggc
    13801 tatctattcg acttacgagt cgaacgaaac catgtacgcc aagctgaaaa cgtacaaaga
    13861 cggtgcctat gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg
    13921 gatgatccag aagatcgaca agtcgaagtt aacgaacttc agcaatctcg atccagacat
    13981 gctcaacaag ccgtttgacc cgaataacga ctactccatt ccgtatatct ggggtgcgac
    14041 ggcgattggc gttaacggtg atgcggtgga tcctaaatct gtcaccagtt gggcagatct
    14101 gtggaagcca gagtacaaag gtagcctgct gctgaccgac gatgcccgtg aagtgttcca
    14161 gatggcgctg cgcaaactgg gctactccgg taacaccacc gatccgaaag agattgaagc
    14221 tgcatataac gagctgaaaa aactgatgcc aaacgtcgca gcgtttaact ccgataaccc
    14281 ggctaacccg tacatggaag gcgaagttaa cctcggcatg atctggaacg gctctgcttt
    14341 cgttgcacgc caggcgggta cgccaattga cgtggtgtgg ccgaaagaag gcggcatttt
    14401 ctggatggac agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cgctgaaatt
    14461 gatcaacttc ctgctgcgcc cggatgtggc aaaacaggtt gccgaaacta tcggttatcc
    14521 aacgccaaac cttgcggcgc gtaagctgtt aagcccggaa gtggcgaacg ataaaacgct
    14581 ctacccggat gctgaaacca ttaaaaatgg tgaatggcag aatgacgttg gcgcagccag
    14641 cagcatttat gaagagtatt atcagaagct gaaagcagga cgttaattca gcaaacgggc
    14701 ggtggtatta ccgcccgttt tttagttagc aaggatgtgg ctaatgagtc aggacagtaa
    14761 agttttatcc cgctctttcg tcggtattgt ggtccccttg taggcctgat aagcgtagcg
    14821 catcaggcaa tgcttcctgc tttataatcc cttcagcaac ttttcaacaa actcaggcac
    14881 cacctggctt gccgggccgt aatatttctc ggcaaattca ttaccgacct gacttggttc
    14941 gagattcagt tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa acccagccgc
    15001 cggataaaca tgcccggaag taccaatggc aatgaaaata tcagccatcg acaacgccat
    15061 ataaatttca tccatgccga gtggcatttc gccaaaccaa acgacgtgcg ggcgcaaggg
    15121 tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg tccagtcgag
    15181 aacctgacca ctttgcgaac aacgaacttt cagcaattcc ccatgcatat gaatgacatt
    15241 ggtattacct gcgcgttcat gcaggttgtc tatattctgc gttaccagca aaaagcgatc
    15301 gccgagggcg tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct gaatttctgg
    15361 ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc accagttcag gatcgcgatc
    15421 gaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc catctgcggc
    15481 gcgaaaggta cgaatacctg attccgcaga aattcctgcc cctgtcagta cgagtactct
    15541 tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct gacgcaaacg
    15601 ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac cgatgacccc gacgcgacag
    15661 catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac gcattcctcc
    15721 cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta agagatgacg
    15781 aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc caccaccaat
    15841 gacgaccagg tcagagtcaa cgatggtcag gatatttccc agacaaaccg ctaataaatc
    15901 cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa gcgcaataat
    15961 ttcgggagcc tgcaacggtt gatgataata gtgttgccag agccacgcaa agccccgacc
    16021 tgatagataa ttttcgatac aaccaatttg accacagccg cagcggcgta acgggaaatc
    16081 cagccccatc atggttaacg catcaaccgg cagacgcata tggccaaact cgccggtaat
    16141 gtagctttta ccggtaatcg gtttaccgtt gaaaatcagc ccgccgccaa cgccggtgcc
    16201 gagaattaac cccatcacca gtggatattg cgtaaattca tcatcccagg cttctgaaag
    16261 ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg cgctcaggtc
    16321 ggcacgcagc ggtttaccgc tggcagcggg gacattggcg gcatacagcg taccatcttc
    16381 tgtttccggc attcccggaa taccgatgcc aacagaacct ttacagccaa aacgttggtc
    16441 agcttcggct accagctcgc atactgcatc taaaaatgcg tcatagctgt cacgcggtgt
    16501 cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccaa gcgcaatttt
    16561 tgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg tactgccttt
    16621 actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc ggataccaac
    16681 ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc agccagtgca
    16741 attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga tgaccgatca
    16801 gcttttcaat ccgctcaata atcggggtaa gttgcagtga aacaactacg ccgataatca
    16861 caccacacag gctaccgaac agccctgcca gcaatccata ccagacaaag atggcgcgaa
    16921 ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc ttgtctttca
    16981 ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc agtaccatcg
    17041 ccagatacat aatggcgcgg atcatctgga tatcgcgata catatagccg taagtaccaa
    17101 tccaactttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt accagcttat
    17161 tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa cccatatcaa
    17221 gatattgttg ggcatccgcc agcgggatca tggcaaaact gtgatcgagt tgaccactca
    17281 actgcaaaat accggcaatg tgcaaacgca cacgttttgg ctgcatcagt ttatgctcgg
    17341 gattcgagtt ggggatcatg atcgacaccc aatcgccctg cttcactttc agcgcatccg
    17401 ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc caggcatccc
    17461 cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgcgggtta acgcccttca
    17521 cctgaattgc gcgcagattg gtaccacttt ctaccagtcc ggtgaaattg atatacggcg
    17581 cagcggcggc aatacccggc actttttgca cgttatccag tgcttcctgc cagttagtcc
    17641 atggctgatt taccgcctca atttcaccgt gcggtaccac agcaagaatg cggttattca
    17701 gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc acgccgaggg
    17761 caatgccaat ggtagaaatg acagagatta gcgacaccat gccgccgcgt cgccgtccgc
    17821 gactaaaacg caggccaatc aataacgata aaggcatcgc cattactccg cccccatcag
    17881 gctcagttcc gccgtcagac gtccatcacg catttccagt tggcggctca tacgtttcgc
    17941 cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat tcaattcccc
    18001 aagcaactgg aagatgctgt cggcgttacg cgcatcgagg ttaccggtag gctcatccgc
    18061 aagtaccaga cgcggattat tgaccagcgc acgggcaatc gccacacgct ggcgttcgcc
    18121 gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg cttttaacat
    18181 ctccagcgca cggctgttga tttcagcggg ctttttcttg ccaatcagca gtggcatagc
    18241 cacgttttcc agggcagtaa aatccggcag caggtggtga aactgataaa taaagcccag
    18301 cttctggttg cgcagttctg ctttcgccgc cgaagacagt ttgctcatcg gttgaccgtt
    18361 aaagatcaca tcgccggagg ttggtgtatc cagcccgccc agcaggtgca gcaaggtact
    18421 tttaccggaa ccagagctac cgacgatcgc catcatttca ccttcgccga cgctgaaact
    18481 gacattgtgc agtacatcgg tttgcacact gccttcctga tagcgtttgc acaggttgtc
    18541 gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgagtg gcggcagcgc
    18601 gccatgaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc gcaataacaa
    18661 tgacctgtaa aggttcgata gcgaccggca gcgccgcgcc atcaagcagg acgccgatta
    18721 tcggcatcag attatttaac tggctggcaa gcagtgcgcc aagcgccgct ccgaggatcg
    18781 caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt tgtcgcggag
    18841 ttaagccttg cgtttgcagg atcgcgactt cgccctgctt ctccattacc atcagcccta
    18901 gtgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagc aagcccatca
    18961 tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc cagtcctgcc
    19021 atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc aactttcagc ggctcatcca
    19081 gccacaaacg ccagccggta atattgcctg ccggataacg catcagacgc gaggcatcct
    19141 caatattcac cagcatttca tagccatcga cttcactgtt ggcggcgaaa gtgccaatca
    19201 cattgaacag gcgctggctt ggaatacgcc ccatcggcgt gaactggctg gcagatggta
    19261 ccatcacgcg gatttgatca ccgcgattaa cgcctagctg tgaggcaagt tgttcgccga
    19321 ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc agatacggcg
    19381 taagtggatc tttttgcgcc gggtcgatac cgagcatcac cccgaccgcc acgctgcgcg
    19441 cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg ccgtccagtt
    19501 tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa gagagaattg
    19561 cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg ttcatcactg
    19621 acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa agccaggaga
    19681 cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca ataaatagag
    19741 cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag tatataagcg
    19801 aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc tgaaaagccc
    19861 ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc tgatcttaat
    19921 ctgaaaaatc atccaataca gacagatacc cattacacct cgggtcataa cccgcaggat
    19981 caaccgcatg taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag agctatggat
    20041 taaccagatc aaagggttat gtatttgtct ggtggtgatt tatcactcgg tcattacctt
    20101 ttatccgcat atgaccactt ttcagcatcc gttatcggaa gtcctgagca aatgctggat
    20161 ctatttcaat ctttaccttg caccctttcg tatgccggtt tttttcttta tatccggcta
    20221 tttgattcgc cgctatatcg acagcgtacc gtggggaaat tgtctcgata aacggatctg
    20281 gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt taagtgcgct
    20341 gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc tccaatgccg cttatgccga
    20401 ttccaccggt gagttcctgc acgggatgat caccgccagc accagcttgt ggtatctgta
    20461 tgcgttaatt gtctatttcg tggtatgtaa aatttttagc cgtctggcgc tgccactatt
    20521 cgccttgttt gtactgctga gtgtggcggt taatttcgtt cccacgccgt ggtggggaat
    20581 gaacagtgtg atccgcaatt tgccttatta cagccttggc gcatggtttg gcgcaacaat
    20641 aatgacctgt gttaaagagg tgccgttgcg ccgccatctg ctgatggctt ctttgcttac
    20701 cgttctggcg gtcggtgcct ggttgtttac tatctcgctg ctgttgtcgc tggtatcgat
    20761 tgtggtgatc atgaagctgt tttatcagta cgagcaacga ttcggtatgc gctccaccag
    20821 cctgctgaat gtgattggct ccaacaccat tgctatctac accacccatc gcattctggt
    20881 tgaaatattc agcttaactc tgcttgcgca aatgaacgca gcacgctggt cgccgcaagt
    20941 cgaactgaca ctcctgctgg tttatccctt tgtcagtttg ttcatctgta ctgttgcggg
    21001 cttgctggta agaaaacttt cacagcgtgc attcagcgat ctgttgttct ccccgccttc
    21061 actgcccgct gccgtcagtt actctcgtta aaccatcgcc ccagccgggg cgattcgatt
    21121 tgctaaacca tgtcgattac ggataatgaa cagcattgct tatcagagat atgcccccat
    21181 atgttgaggc atatcctaac gagaatctga caaccgttat gcctgaacaa tatcgttata
    21241 cactgcccgt caaagcgggt gagcagcgtc tgctgggcga gttaaccggc gcagcctgtg
    21301 caacgctggt agcggaaatt gccgaacgtc acgccggtcc ggtggtactc attgcaccag
    21361 atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca gttcaccgat cagatggtga
    21421 tgaatctggc ggactgggaa actcttccct acgacagttt ttcgcctcat caggacatta
    21481 tctcctcgcg cctttccacc ctttaccagc taccgacgat gcagcgtggc gtactgattg
    21541 ttccggtgaa tacgcttatg cagcgcgttt gcccacacag ttttctccac ggtcatgcgc
    21601 tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa ctggacagcg
    21661 ccggttatcg ccatgttgac caggtgatgg agcacggcga atacgccacg cgcggcgcgt
    21721 tgctggatct cttcccgatg gggagtgagc tgccttatcg tcttgatttc tttgatgatg
    21781 aaatcgacag cctgcgggtg tttgacgtcg acagccagcg cacgctggag gaagtagaag
    21841 cgatcaatct gctgcccgcg cacgaatttc cgaccgataa agcggcaatt gaactgttcc
    21901 gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt taccagcaag
    21961 tgagtaaagg cacattacct gccgggatcg agtactggca gccattgttc ttcagcgaac
    22021 cactgccgcc gctgttcagt tatttccctg ccaatacctt gctcgtgaat actggcgatc
    22081 tggaaaccag tgccgaacgt ttccaggctg acacgctggc gcgttttgag aatcgcggcg
    22141 tcgatccgat gcgcccgctg ttgccaccac aatcgctctg gctgcgggtg gacgagctct
    22201 tctcagagct gaaaaactgg ccccgggtgc agctaaaaac tgaacattta ccgacaaaag
    22261 ccgcgaatgc caatttaggt ttccagaaac tgccagacct ggccgttcag gcacaacaaa
    22321 aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt cgacggtccg gtggtgttct
    22381 cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact gctcgcacga attaaaattg
    22441 ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg tgggcgttat ctgatgattg
    22501 gcgctgccga acatggtttt gtcgataagg tgcgtaatct ggcgctgatc tgcgaaagcg
    22561 atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc tcgccgcacc atcaaccccg
    22621 atacactgat ccgtaacctt gcggagctgc atattggtca gccggtggtc catctggagc
    22681 acggtgtcgg gcgctatgcc ggaatgacca cgctcgaagc gggcggcatt actggcgagt
    22741 atttgatgct cacctatgcc aacgacgcca aactgtatgt tccggtgtcg tcactgcatc
    22801 tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc gctgcataaa cttggcggcg
    22861 atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg gcggcggaat
    22921 tgctggatat ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa cacgatcgtg
    22981 agcagtatca gttgttctgc gacagcttcc cgtttgaaac cacgccggat caggcgcagg
    23041 ccattaatgc ggtacttagc gacatgtgtc agccgctggc aatggatcgt ctggtgtgcg
    23101 gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc cgctttcctg gcagtagata
    23161 accacaagca ggtggcggtg ttggtgccta ccacccttct cgcgcagcag cattacgaca
    23221 acttccgcga ccgtttcgcc aactggccgg tacgtatcga aatgatctcc cgtttccgca
    23281 gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga agggaaaatc gatattctga
    23341 tcggtacgca caaactgctg caaagtgacg tcaagtttaa agatttaggc ctgctgattg
    23401 tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg cattaaagcg atgcgcgcga
    23461 acgtggatat tctgacgctt actgcaacgc cgatcccacg cacgctgaat atggcaatga
    23521 gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc ccgtcgtctg gcagttaaaa
    23581 cctttgtccg tgagtatgac agcctggtgg tccgggaggc gatcctgcgt gaaattttgc
    23641 gcggggggca ggtttattat ctctacaatg atgtggaaaa catccagaaa gctgccgaac
    23701 ggctggcaga actggtgcca gaagcgcgga tcgccatcgg tcacgggcag atgcgcgagc
    23761 gcgaactgga acgggtgatg aatgatttcc atcatcaacg tttcaacgtg ctggtttgta
    23821 caaccattat cgaaaccggg atcgacatcc cgacagccaa caccattatc attgaacgcg
    23881 cggatcactt cggtctggcg cagctgcacc agttacgcgg tcgcgtcgga cgttcgcatc
    23941 atcaggcata tgcatggttg ctgacaccgc atccaaaagc gatgactacc gatgcacaaa
    24001 aacgtcttga agcgattgcc tcgctggaag atctcggggc aggttttgcg ctggcaacgc
    24061 acgatctgga gattcgcggc gcgggtgaac tgcttggcga agaacaaagc ggctcaatgg
    24121 aaaccatcgg tttctcgctg tatatggagt tgctggaaaa cgccgtcgat gcactgaaag
    24181 ccggacgcga gccgtcgctg gaagatctca ccagccagca aacagaagtc gagctgcgga
    24241 tgccgtcgct attgccagat gatttcattc ctgacgtgaa tacgcgtttg tcgttctata
    24301 aacgtattgc cagcgccaaa acggaaaacg aactggaaga gatcaaagtc gagcttatcg
    24361 atcgcttcgg cctgctgcca gatccggcgc gtaccctgct ggatattgcc cgtctgcgcc
    24421 agcaagcgca gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc ggggtgatcg
    24481 aatttgccga gaagaatcac gttaatccgg cctggttgat tggtttgctg caaaaacagc
    24541 cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt tattcaggat ttgagtgagc
    24601 ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga actggaagag aacgcgatcg
    24661 cttaatggta aatcgggggc gtttctgcgc ccccatctgc aacatttaca aattctttgc
    24721 acttccctgc actatccgac actgtcacca tccataattc aggcttatct gtttattaca
    24781 ataaccttat atttattatt gatttttggt gatgatcaaa acgcgttttt ctcgctggct
    24841 aacgtttttt acgttcgccg ctgccgtggc gctaccggca aaagccaaca cctagccgct
    24901 gccgccagcg agcagtcgtc tggttggcga aaacaaattt catgtggtgg aaaatgacgg
    24961 tggttctctg gaagccatcg ccaaaaaata taacgtcggc tttctcgctc tgttacaggc
    25021 taaccccggc gttgatcctt acgtaccgcg cgcgggcagc gtgttaacga tcccgttgca
    25081 aaccctactt ccagacgcgc cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct
    25141 ctattactac ccgccgggta aaaattcggt aaccgtgtat ccaataggta ttggtcagtt
    25201 aggtggtgac acgctgacac cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc
    25261 aacctggacg ccaacggcaa acatccgcgc ccgttataaa gcacagggaa ttgagttgcc
    25321 tacggtagtg ccggctggac cggataaccc aatgggccat catgcgattc gtctggcggc
    25381 ctatggcggc gtttatttgc ttcacggtac gaacgccgat ttcggcattg gcatgcgggt
    25441 aagttctggc tgtattcgtc tgcgggatga cgatatcaaa acactcttta gccaggtcac
    25501 cccaggcacc aaagtgaata tcatcaacac tccgataaaa gtctctgccg aaccaaacgg
    25561 tgcgcgtctg gttgaagtac atcagccgct gtcagagaag attgatgacg atccgcagct
    25621 gctgccaatt acgctgaata gcgcaatgca atcatttaaa gatgcagcac aaactgacgc
    25681 tgaagtgatg caacatgtga tggatgtccg ttccgggatg ccggtggatg tccgccgtca
    25741 tcaagtgagc ccacaaactc tgtaaatcct ggataaaaaa aatccccgcg acacggcatg
    25801 tcgcggggaa aaaatcagca gtagcaggca ttaatgaggg ttaatgctta tttataaatt
    25861 actgctgttc catggagggt attcggaccg gttacagaag taatacggaa agattttgcg
    25921 cccatctcat ccgctttttg cgccagctgc tcttccagcg atcccagatt tgtccccgcg
    25981 ttagcactga tagtaccgac tttttgttgg ccttctggcg ttgactgaac ttcgacagcc
    26041 gcaaagttgg caaatgacat ggagcttaaa atcgccgcag cgatgagggt ttttacgttt
    26101 ttcataatag tggccttatg cagatgaatg acgtcgcaag aaattactta acgatcgata
    26161 actaaatga
//
LOCUS       DAZOYS010000038        25808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOYS010000038 DAZOYS010000000
VERSION     DAZOYS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.038"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUN9_001385"
     CDS             complement(<1..215)
                     /locus_tag="ACHUN9_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0694824.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUN9_001386"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUN9_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0694825.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUN9_001387"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUN9_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0694826.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUN9_001388"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUN9_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0694827.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUN9_001389"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUN9_001389"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0694828.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUN9_001390"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUN9_001390"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0694829.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUN9_001391"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUN9_001391"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0694830.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUN9_001392"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUN9_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0694831.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUN9_001393"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUN9_001393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0694832.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUN9_001394"
     CDS             6526..6744
                     /locus_tag="ACHUN9_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0694833.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUN9_001395"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUN9_001395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0694834.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUN9_001396"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUN9_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0694835.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUN9_001397"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUN9_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0694836.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUN9_001398"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUN9_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0694837.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUN9_001399"
     CDS             10175..12088
                     /locus_tag="ACHUN9_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0694838.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUN9_001400"
     CDS             12088..13110
                     /locus_tag="ACHUN9_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0694839.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUN9_001401"
     CDS             13104..13322
                     /locus_tag="ACHUN9_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0694840.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUN9_001402"
     CDS             13376..14245
                     /locus_tag="ACHUN9_001402"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0694841.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUN9_001403"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUN9_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0694842.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUN9_001404"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUN9_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0694843.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHUN9_001405"
     CDS             15689..17779
                     /locus_tag="ACHUN9_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0694844.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUN9_001406"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUN9_001406"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0694845.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUN9_001407"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUN9_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0694846.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUN9_001408"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUN9_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0694847.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUN9_001409"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUN9_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0694848.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUN9_001410"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUN9_001410"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0694849.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUN9_001411"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUN9_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0694850.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUN9_001412"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUN9_001412"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0694851.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUN9_001413"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUN9_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0694852.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6291 a         6986 c         6463 g         6068 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caagcgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctg
//
LOCUS       DAZOYS010000039        25734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOYS010000039 DAZOYS010000000
VERSION     DAZOYS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.039"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            27..446
                     /locus_tag="ACHUN9_001414"
     CDS             27..446
                     /locus_tag="ACHUN9_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694853.1"
                     /translation="MNVKIRNEIRALIRIKERNNNGGDLREFICAREVEGYGEKTYLI
                     AFDHYSICARYCGEAVFRAITFGNAFNVDLWEYVMDREYICASDPKAREMWQRIWRDY
                     RLMAKGWARCCYSSLVLKAVQLSLRHIPASLREPLLY"
     gene            459..746
                     /locus_tag="ACHUN9_001415"
     CDS             459..746
                     /locus_tag="ACHUN9_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694854.1"
                     /translation="MTCNRKRWSREDREFIEASVGKMTVEEMAKKLKVAPTALQAHAR
                     RHGISLCAYRVSEHDKYLCRELYKEGLAIHVIAKKMELSNRAVSGIVYSEY"
     gene            765..1289
                     /locus_tag="ACHUN9_001416"
     CDS             765..1289
                     /locus_tag="ACHUN9_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0694855.1"
                     /translation="MAKPAKRIRNAAAAYVPQSRDAVVCDIRRIGDLQREAARLETEM
                     NDAIAEITEKYASQIAPLKTSIETLSKGVQGWCEANRDELTNGGKVKTANLVTGDVSW
                     RQRPPSVSIRGVDAVMETLERLGLQRFIRTKQEINKEAILLEPKAVAGVAGITVKSGI
                     EDFSIIPFEQEAGI"
     gene            1387..1935
                     /locus_tag="ACHUN9_001417"
     CDS             1387..1935
                     /locus_tag="ACHUN9_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFR0694856.1"
                     /translation="MKAYAYFKLSDAAANGYTIRWLKDVSVKRDKIICSLQRAFGADG
                     VSLVSNRGYERVECIWMNQLAPELWRGKSDGLFIGGFQFYGVVPDITTPEGRNNATLI
                     QEHEEQLRKYPTFPVWLCNELGVDVVPNMFDLKSVWTMHHSRDGFGILFEVCLLDGEV
                     KGPVPAECQRIKHSEFVALTEE"
     gene            1939..2478
                     /locus_tag="ACHUN9_001418"
     CDS             1939..2478
                     /locus_tag="ACHUN9_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694857.1"
                     /translation="MIDAKVLNGVSTLLRAYGRLTCGVLAEKMNMLPSSMVYFLRDAV
                     DAGVLTECNGFYDVPRPRLTSPVRRNTTELPGVDDAAWCTFRRSLPWLEGNAIPALAK
                     EFATGVLTCESVYIVAEVDDEMCKQGMPRFVMAYIDIRLGRFICGSSGWNITDHVLRY
                     LILDYSPVPAETQEISGHD"
     gene            2471..2653
                     /locus_tag="ACHUN9_001419"
     CDS             2471..2653
                     /locus_tag="ACHUN9_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694858.1"
                     /translation="MIKSIGFVLLVGTCGNDACDAIPVTEKIWPTEQACMQVMERIQK
                     RYPGEIFYCEDALRNE"
     gene            2646..3008
                     /locus_tag="ACHUN9_001420"
     CDS             2646..3008
                     /locus_tag="ACHUN9_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694859.1"
                     /translation="MSKRIYDDPKYRAEMRAQLVVSGYTPEQADATLTELMGERPFDE
                     AEYEYWLKQFRLEILENTTLPSLIRIIKSVIDNACYNGDCLAAAIATHALVNLLEVTG
                     AVFLNPEKVLRDENTDDY"
     gene            2986..3321
                     /locus_tag="ACHUN9_001421"
     CDS             2986..3321
                     /locus_tag="ACHUN9_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694860.1"
                     /translation="MKIQMITDADLMAATLISSGMSEQKVNDFLDNVVYQRYEQAREN
                     LISEKRTMIAESLSPAWIIEELRKIIASQDKVAAVAALDVLHRMSKELFNTELILNPG
                     GHNEPHIPD"
     gene            3299..3850
                     /locus_tag="ACHUN9_001422"
     CDS             3299..3850
                     /locus_tag="ACHUN9_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFR0694861.1"
                     /translation="MSRTSLIKLIHVARRDLQLDDDTYRTFLVQCTGKTSCRELSVAQ
                     LERVLDAMKERGFKKQKKHPRRRFKGHVTPREKIYKIWQQMFLDGFVSDISDAALDKY
                     VERLTTRRNGGQGVSTLAWCHGESLQVVLETLKQWHMRCIREAFARHGVPLPVSKTGQ
                     ELRGYDALTSAYARARNSGRIAV"
     gene            3847..4296
                     /locus_tag="ACHUN9_001423"
     CDS             3847..4296
                     /locus_tag="ACHUN9_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFR0694862.1"
                     /translation="MKQMKEQDLFEDFRDDSVLEYLNDYQENTAYPALLSELNALLRK
                     ELARIGADPAHSLELVVAICRHIGGMQVYVPRGNILENLVRDMRIWRDFNGHNIPELV
                     QRYGVTYKTVYKAIKRMRRLECNKYQPDLFYHSPNLPHPVRRPPRRP"
     gene            4382..4969
                     /locus_tag="ACHUN9_001424"
     CDS             4382..4969
                     /locus_tag="ACHUN9_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFR0694863.1"
                     /translation="MENLKFSPAFEHALNFILRPDIEGVYVNDPTDRGGETRYGISDR
                     RDGVIDGKTDVSGDGKPDTRIRDLTREQVAQIYWRDYWLPAGCDQWPDGVAMFVFDAA
                     VQHGVKKAIRILQEAADVDADGIIGPRTRKAVSLSTPDWLLTRCIVRRSRFYADIIKS
                     KPAQGKYLNGWFNRMEKVTDACLEIIDNVSSVTRG"
     gene            4971..5198
                     /locus_tag="ACHUN9_001425"
     CDS             4971..5198
                     /locus_tag="ACHUN9_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694864.1"
                     /translation="MGKGWDASLRAGRRDRLRQEVLHRMAGGPPPKPLDYTGHDGTHA
                     SHYMRGWNTVDTRDIFWQCQKYKEKLNVQNE"
     gene            5182..5592
                     /locus_tag="ACHUN9_001426"
     CDS             5182..5592
                     /locus_tag="ACHUN9_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694865.1"
                     /translation="MYRMNSLFVRVIYSRFMLCQWSFLGLSAVLFLASCFAGWQAFAV
                     MVLAYGATLCLIHSFQLGNVPYRLQPKRRFRAASLRIVVWSWGVWAVGFFMLTFGLLY
                     LKEPYQLAFWLGGIFCAALYKHQRRFHKGDSWTR"
     gene            5580..6188
                     /locus_tag="ACHUN9_001427"
     CDS             5580..6188
                     /locus_tag="ACHUN9_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694866.1"
                     /translation="MDPLTLSGIASVLLKAGPGLIRSVGRWFGGSTSAAADSVAGMVE
                     SVRESLPVAEQQRILEQKLVMLSPEQLVQLETLKVQLQQLEVERQKLVLADQQAAHHE
                     QQETIRNGDNATDSYVRQTRPLLARLSCYSSLAYVLLLSCGQIAGAVAGANGITLHMP
                     SPDWDITLMLFTPALSYLGVRTLDGFARYSKSSCHKISAGPK"
     gene            6185..6412
                     /locus_tag="ACHUN9_001428"
     CDS             6185..6412
                     /locus_tag="ACHUN9_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFR0694867.1"
                     /translation="MTDETDRISEVVLNERQDVVNAWLMRIKEAPHSRGFCNDCGNVI
                     PAQRIAVLPDVVTCIDCQQARERRRKTCPGK"
     gene            6397..6702
                     /locus_tag="ACHUN9_001429"
     CDS             6397..6702
                     /locus_tag="ACHUN9_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFR0694868.1"
                     /translation="MSWEVIRANWPVLWALLMTAINLIQLILAKTYVKREEFDSLRSR
                     VSVMEGLVNQLPDRNEFHRLQLDISNLRGEIKELGPSIRQVSRISDLLLENELKEKN"
     gene            6708..6995
                     /locus_tag="ACHUN9_001430"
     CDS             6708..6995
                     /locus_tag="ACHUN9_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFR0694869.1"
                     /translation="MKEILTEDRRLVLLRSLLDCGDSANESVLQTCLQAYGHKVSRDV
                     VRTQLAWLREQGLVRLSDVGGCYVAEITGSGEDVANGLSGVPGVKKPRARD"
     gene            6998..7285
                     /locus_tag="ACHUN9_001431"
     CDS             6998..7285
                     /locus_tag="ACHUN9_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694870.1"
                     /translation="MARKLKPLSRGERAVVRQLAYCLVLADIEQNAIVRAYEKHTGKP
                     WNPDAPDTPVKRALRSSPAYARLWKLPGKDIQSVREEIYAGLKTQRTEDGK"
     gene            7275..7856
                     /locus_tag="ACHUN9_001432"
     CDS             7275..7856
                     /locus_tag="ACHUN9_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HFR0694871.1"
                     /translation="MENEQRPTRGRLSKVDLLPDSIREQLHQMLREKRHTQEEIREAI
                     NALIDEHNLPGEMQLSRTGLNRYASRMEKVGAKIRASREMAEVWAAKLGSAPTSDVGR
                     LLMEFVKTLAFETSMSMTEDDKPVAPKALGQLALVAQRLEAAAMTSHKREKAIRDAFA
                     QEMAEKTEELVRAGGLSGGAADTIKREILGISV"
     gene            7853..9388
                     /locus_tag="ACHUN9_001433"
     CDS             7853..9388
                     /locus_tag="ACHUN9_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0694872.1"
                     /translation="MTQMNAFSESDVLLPYQKRWVADDSKLKIAEKSRRTGLTWAEAA
                     DAALTASLKKENGGRDHFYIGSNKEMAREFIDAVAMWAKAFNAAAEEIREEVITDEDK
                     DILTFVVYFASGFKVKALSSNPGNIRGMQGNVTIDEAAFHEKLDEVLKAVTPLTTWGG
                     KVRLISTHDGVDNPFNQLIQESRAGKKDYRIHTITLDDACNDGLYRRICQVRGMVWSP
                     EAEVEWKEGLLRNTATREDALEEYYCVPKNGGGTYIPRSLRERAARGTGKVLRFTGTP
                     EFNALTEGQRRAEIREWLETVVRPELEKLPKNLRHCLGEDFARSGDLTVLAPVAVNDD
                     TTREVPFLVELANVPFKQQEQVLFWLCDRLPRRDGIKMDARGNGQYLAEQAAERYGDE
                     VEQVMLSVAFYRENMPRFRAAFEDDELILPKHEDVINDLGAIQLLRGVPGIDDARTKG
                     SDGRKRHGDAAVAIFLGFLASKDDCHRYELHRLNRPAKPEEGNARRQMKLTRGLKNEG
                     GLL"
     gene            9385..10956
                     /locus_tag="ACHUN9_001434"
     CDS             9385..10956
                     /locus_tag="ACHUN9_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFR0694873.1"
                     /translation="MNLKQLAGAVRRLLNPATGEEDTLKKEALNEAQARPRHAGVRPT
                     SPGISVASGLNPGRLAGILRNAADGITRDFFILAEEMEERDLHYASVLRTRKLTVAGI
                     EPVVVAASDDDADVQLADAIRALMEAPQIPELMFDLLDGLGKGVAVCEILWDTRNNHW
                     MPRDYEWVDPRFLKAEKPTLRQFRLLTDDEPVDGVPLTPGKFIVHQPRLKSGLPLRNG
                     LARLVAVMYMLKSFTVRDWWAFAEKFGIPVTVGKYGPNATEEQIRVLIDAIASIASDA
                     GCAIPQSMQLEMQETASRNNGGALFREMAEWCDAQISKAVLGQTMTTDNGSSRSQADV
                     HNQVRMDIVRWDARQLANTLNEYLVRPYIEANYGPQAHYPRVVLRISEAEDLKALTDA
                     LVPLIDRGMRVQESELRDRFGLAEPDEGADVLHPVSAGMPGDMAMNRERVALNREHPD
                     ELAQMVDDALRDWQKTGEAFTNPVLTLAQECDSFDDFLKRLPELQETLNADDFALQLA
                     EVCFKARALGDTAHA"
     gene            10949..11713
                     /locus_tag="ACHUN9_001435"
     CDS             10949..11713
                     /locus_tag="ACHUN9_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0694874.1"
                     /translation="MRETLIPKEALAWLKAKKLRPGFDYRDVWREEHRNSFTVAKMLQ
                     LDLLSDVKALVEDALQSGQTFSEFREMLQPLLIKRGWWGVQEMDDPLTGETRTVQLGS
                     DRRLRTIFDTNMRTARAAGQWERIQRTKQAMPYLMYELGPSREHRVEHVKWARLCLPV
                     DHPFWQTHFAPNGWGCKCTIRQVSRGEYAQLAAQGTIHTEAPEIRTVRWVNKRTGEEE
                     DVPEGIDPGWNYNPGINREQELARQLAARQARFNSE"
     gene            11928..12884
                     /locus_tag="ACHUN9_001436"
     CDS             11928..12884
                     /locus_tag="ACHUN9_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFR0694875.1"
                     /translation="MQPELLALCFSLPEPIPELTPAQLPEWLELVPAGEFTGRDGRTW
                     MNRNPHEVVARSSDINIPVDIEHATEIKGPRGEEAPAYGWVEALRVTDSGTIEGRVVW
                     SESARWMLSERRYRYYSPAFFHDADGAVTRLSSVGLTNKPNLDFPALNTEKNPMTVPV
                     QITGLLGLAESATVDDTVAAIKQLQENEQVALNRAQTPDLTKFVPVETHNLALNRAET
                     AEKRLQQLEEKEAEALVDAAIGAGKVAPANRDMFLATCRTEEGRKQFAEYTKGAQPLV
                     NNDKPSQGKDKPAQTLTDAELAMCRSMGITGEEFLAAKPKQE"
     gene            12892..13797
                     /locus_tag="ACHUN9_001437"
     CDS             12892..13797
                     /locus_tag="ACHUN9_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFR0694876.1"
                     /translation="MSQTISSEILHALTTCLSAAFTRGLSGVEPQWNRIASEVPSSSA
                     SNTYGWMKDLPDIKEWVGERQLATLDGYGYTITNKLWESSIRVKREHIEDDQIGQYSI
                     TAERYGRMTAVFPDKLCYALLCAGFNTLCFDGQNFFDEDHPLGDGTYSNVVGTPASDK
                     GEPWFLIDESQVLKPIIWQTRRAFKFEALDDLNSEHTFKNHEFLYGVDGRCNAGFGFW
                     QTAVGSHAALTVENYKKANELLRGMKGTNGEPLGIRPTTLVVGPKNRADAKLIIDAML
                     VNGGDSNIWYKDVKIVDSPYITTPA"
     gene            13869..14180
                     /locus_tag="ACHUN9_001438"
     CDS             13869..14180
                     /locus_tag="ACHUN9_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694877.1"
                     /translation="MSEKAETKGAKAAKNSAAQENPAPLADVIVADGQANEPRPAEDP
                     VAVQGDAPVRLNVRAVSENGFWRCGRFWSHIGEDVAVSAAVATRLMAEPNLIVREAEK
                     G"
     gene            14180..14617
                     /locus_tag="ACHUN9_001439"
     CDS             14180..14617
                     /locus_tag="ACHUN9_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HFR0694878.1"
                     /translation="MGYITQEDLLRADGNLVWNMAINRETNGLDEDKIRQAISDAEAE
                     IDSFLSRRYQLPLGVTEIPRPLQRVAVSLAFYWLSERDNQITELIQKRYDDAIKTLRE
                     MANGTRDLGLPTYATPAETDNGKIIVVGANARLFTRNNLKGVL"
     gene            14617..15159
                     /locus_tag="ACHUN9_001440"
     CDS             14617..15159
                     /locus_tag="ACHUN9_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR0694879.1"
                     /translation="MGISVQVSGDQRLEDIRRAVEKLAEGSLQSELLESIGAVVESQT
                     RRRIIDEKTSPGGERWPDWSDGYKKTRHGNQSLLRGEGHLLESIQYIVENRVVRIGSP
                     LDYARIMNDGFSGSVPVSAHKRLISQCFGRALKYPVWQTVGAHHRMMNIPQREFLGLS
                     SASQQELQQVISHFWKEVLP"
     gene            15156..15767
                     /locus_tag="ACHUN9_001441"
     CDS             15156..15767
                     /locus_tag="ACHUN9_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694880.1"
                     /translation="MTEQRPELRTPGSTVAAAERIVAWLQTALQGDTPDRADVVERHI
                     GQFNSTDEVKRYLSGRTGCIRVAALRVRDINPRGGLSGLVTWVAYIMATDSWGYSRDV
                     RCEVLAGKVIKRLLSSDATGGMGAERMAADVRADNIYSVSLDGLGVTMWAVTWEQEFR
                     LDEEIDLAALPDFLRLGATLRSGEHTEINDVIHVRGDDGTETD"
     gene            15748..15933
                     /locus_tag="ACHUN9_001442"
     CDS             15748..15933
                     /locus_tag="ACHUN9_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR0694881.1"
                     /translation="MEQKLIKPARENVRVRKPDGAHLSPEGERLNVCAYWLRREAEGD
                     VEITTLPKNNNKTRGKK"
     gene            15933..17408
                     /locus_tag="ACHUN9_001443"
     CDS             15933..17408
                     /locus_tag="ACHUN9_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0694882.1"
                     /translation="MSLGSIPDDIRVPLVWIDIDNSQALEGASAQSRKILVMGHAVSS
                     GSADALSLTRITSDSQADQLYGKGSMLAEMLKMLRRANTYTETWAMPVAAPEGAAAKA
                     TLTVLGTATEAGTVALLINGVSVQVSVSAGATKENIAKAIADAVTKKPATQVAAAVKD
                     DATDTVELTVNWHGVTGNGADVRLNYHTGEAFPAGVKVTATAFTGGTGTPEMADAVAA
                     IGPEWFTDIIAPFTDTKSLNTLRDELLNRWGPLKMMEAQLWTAFRGTHGETGTFGETR
                     NDWLISCIGTNLSPHPAWMWAASYGATAAYHLAIDPARPLQTLVLTGILPPARNVRWD
                     MPERNLLLHDGIATHMVDAGDNVCIEREITMYRVNQYGDADVSYLDVQSPATLGRIRY
                     IIKNRFTNRYPRHKLADDDVLDSLDAGQPVMTPKLCTAELLDICQTELLPAGLVENFS
                     DYKDTLQVTRDSSDKNRLNFICHPNLVNQLRVLAGLIQFKL"
     gene            17418..17774
                     /locus_tag="ACHUN9_001444"
     CDS             17418..17774
                     /locus_tag="ACHUN9_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0694883.1"
                     /translation="MAKILGMATIRVNGREIKTEGKSTLNPGGFSRTQHMGGGKVWGI
                     SSKMASPSIKVTIAAAADMDVIEISSWEDVTVMFYGNNGLNYMMTGSATDNPAELDED
                     SGTISANFIGEKCVKV"
     gene            17776..18171
                     /locus_tag="ACHUN9_001445"
     CDS             17776..18171
                     /locus_tag="ACHUN9_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0694884.1"
                     /translation="MAAMEFELKHGLLTGKGTADETLHKTVKLRELTASDVIDAQLAA
                     ERVVMGGNGKAVAYCSEVLMGLEMMRRQVASIGNIPGPLDMKQLRMLHPADLELISTK
                     AAALDEMLEEVATRGRTDAAGGGSDEPAG"
     gene            18258..20051
                     /locus_tag="ACHUN9_001446"
     CDS             18258..20051
                     /locus_tag="ACHUN9_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0694885.1"
                     /translation="MATGNRLSTEIMINLAGNLTAKARQYGANMSQFARNNQKAMRLV
                     KATTEAAGRGLDTLGNRYTAMIAGLGSSLTIKQVADFDAQMRRMGTDAQLTTEQVKTL
                     HDKIRDVSNQKDIRIDASALGQGASELLGKTGDYQYVVDNLRNMGLMMQAFGVDGQVA
                     AGLMAQFWEKNVRGADAVNNMMDRLYSQFAVGSVSVADVARAAPKLFSIIQDQGPEAI
                     AQMGAFAQVFAKNKGSIDETVTSIQAMYASLSDKKNIEFLKKNGIDVFVKGTKDIKKP
                     YELMMEILKRAKYDPLKLQDVFDLTGMQGIKALLNPENRQLMEQMIYGTIELGSTQKA
                     AQTNAEGFNAAIQSLNNEWQRFAEGQLAKPVQDLADALNSVDQNTAQNWLQVGKYMAI
                     ALGGIIAIRKTYQFGKTIHDIMNPKGKGKGIPGSITDVFGSGVMPVYVVNMGSGGMKG
                     NTGGLPDAPDSSRNPRNSRNPRGPKTPALVTPQTASGFNLLDMVLSAFPKNKEEADAL
                     IARVQENNNRPTVWTDIRNWFNSLEKKNIADPRPWAGMQPTQNYPFLPQQLQGEIRVV
                     VEGDARVKSVRVDQPGVRLSAQAGVTSVEQG"
     gene            20051..21325
                     /locus_tag="ACHUN9_001447"
     CDS             20051..21325
                     /locus_tag="ACHUN9_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0694886.1"
                     /translation="MTTSNGKWDGLRDASFRGVPFFLVDTEGTGGRRAIPRAYPRRET
                     AWTDDNGAVPGQQQINAKLPGKNFRDDLNALLDALNTPGPGELVHPWFGIQTVQVGKV
                     THRLSTEEDGIAYVTFEVFEAGERLFPSAADNTQQEVLTGIDAVKAAIDDGDWFGALD
                     GLGDMADSFLADMENLVANLPTLPAALNQWMDRLKHFKAMAGTLIATPGRLVSELSAR
                     VEDIAGLVTEPPEALAVYTTLRNQWAGERARQIATGALPEDITVKPGSVADGNPGFAI
                     GLSPDYQPVSDSLQKNIDDFRQVVVLGALLGQASAVASMTFDTRDAALSAGDTLAAEL
                     HEQAVAAVENNQRTLWRTLRDLRQAVITDARERAARLPETRQVTLTTTTSAALLAWRE
                     HGDTRRRDEIVQRNRLRHPSFILPTQPVEITD"
     gene            21325..22395
                     /locus_tag="ACHUN9_001448"
     CDS             21325..22395
                     /locus_tag="ACHUN9_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0694887.1"
                     /translation="MESVILTVDGKLWEGWTEMSVSRSLKAIAGEFELSVTTRWSAAA
                     PRVIREGQPCTVRLGADTVLTGYIDDFIPGYDAENVEIRVMGRDKTGDLVDCSVVHSS
                     GKWKGVRLEQVAADVCRPFGITVITETPTGEAFASVVLEQGETGFELLDRLAKQRGVL
                     LTSDGAGNLIITRASSVRAGVSLVLGKNILAARGRFSWRERNSQYIIKGTTSAGGKLW
                     DSQPAPMVGGRQYITEDPEINRYRPRILVNEDSLTVGGASIRGEWYKARMLGESRTTE
                     ITVAGWREQGDSGPLWQTNRLVDIDDSIQNLKTTWLISGVTWTDGAQGRMTVLALVPP
                     ESLDMPEMKAKTKKTKAVATWD"
     gene            22386..22922
                     /locus_tag="ACHUN9_001449"
     CDS             22386..22922
                     /locus_tag="ACHUN9_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0694888.1"
                     /translation="MGLNAIARRLRLMVDRAVVRLVSDGLARQNLQIQTLADATDDDV
                     ERFQNYGFTSVPPEGSEAIVLAVGGRRAGLVAIAVEDKRCRPKGLSPGDVRVYHRDGK
                     SHITLKENGVIEITGEQVDVSGKTVNLTADELLNIIGKQMKFVGPCEFTEDVRIKGKS
                     FSEHIHKDGDNEKTSPPV"
     gene            22919..23371
                     /locus_tag="ACHUN9_001450"
     CDS             22919..23371
                     /locus_tag="ACHUN9_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0694889.1"
                     /translation="MTTEIRWNNMLSRGDITVTHNGLSRDEGLVTQVLICLFTDARAD
                     DDDVIPDGSGDPRGWPGDTYSDFSWGSRLWLLEREKLTEDVRLRVEDYARLSMQPLLR
                     AGYARNATVTASIIIPDRIAFLVVLTRPDKTTLRIEITRRWEATINAV"
     gene            23361..24437
                     /locus_tag="ACHUN9_001451"
     CDS             23361..24437
                     /locus_tag="ACHUN9_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0694890.1"
                     /translation="MPYEIPTLGKLIADGEKDIAYELGLQKLPPVSVEQALNVSFSSQ
                     VRDLYDHQSWIKDQIIPSTTSDDETIIKTAKYEGVIRKQATFASGRVTFTSQSPLPAE
                     TRMQSDTNQVYQVLTSGEVQDGEVTVIVQAEEAGVAGNLAAGAVLTLLSPLPGTASTG
                     AVAEGGITGGADTESIAALLDRLLYVRRNPPTGGALHDYVIWAREVPGVSRAWAWDAW
                     HGPGTVGLAWLYDDRENIVPTREDLKTMEQYLFCHKHPATGVMVGKPGGIEVWPVQVR
                     LKKVSISIRLTPDSLANRSAVRANLTALQKTLAPGQTLPVSSLRTAIGMTSGITDYFL
                     NIGEDTTSDVDELITIGEVTWLTA"
     gene            24422..25006
                     /locus_tag="ACHUN9_001452"
     CDS             24422..25006
                     /locus_tag="ACHUN9_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0694891.1"
                     /translation="MAHSVTEWLAALQQVMPRGKAWPRDNDADLNRFLRALAERLNRT
                     EYDASRLHVEMRPETTLRLLPEWEQYLALPECGIAPTTREARRRAVVEKYRRRGGLAT
                     WQTEAAAAALGFTIKVTAVLPHHCLRDCMYPLHPARYRWMLKVNVPGKDAGRFTCIDG
                     VMTPLISERTRELECLLKHYRLAGTEYEYYYTGE"
     gene            25010..>25734
                     /locus_tag="ACHUN9_001453"
     CDS             25010..>25734
                     /locus_tag="ACHUN9_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0694892.1"
                     /translation="MFYVDNPTGVPVMPPVAAELSKTTLYFTEGGNGIPPTYPGPDWF
                     NIIQSELLEILRQANIKPDKNTTDQIMTALKKLFITNSGSAGAIAGLTGQNNTFPYFT
                     GKDTMALTPLSAFVRGILGKESATDFADALKVIKQSGGTMTGELKIRGVNALRIFNEA
                     FGLIFRRSEECLHLIPTSEGQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNG
                     ALGIGVQNALGGNSIVLGDNDTG"
BASE COUNT         6043 a         6728 c         7705 g         5258 t
ORIGIN      
        1 acatgccact gaaaggaacg ctgacaatga acgtcaaaat ccgaaatgaa atccgggcat
       61 taattcggat taaagagcgt aataacaatg gtggagactt gcgcgaattt atttgtgcgc
      121 gcgaagttga ggggtatggc gaaaagactt acctgattgc atttgaccat tacagcattt
      181 gtgcgcgtta ttgcggagag gcggtattcc gcgccattac gtttggcaat gccttcaatg
      241 tggatttatg ggaatacgtc atggaccgcg aatacatctg cgcgtcagac ccgaaagccc
      301 gtgaaatgtg gcagcgtatc tggcgcgatt accggttaat ggcaaaaggc tgggcgcgct
      361 gctgttattc ctcgcttgtc ctgaaagcgg ttcagttatc gctgcggcat attccggcat
      421 cactgcgcga gcctctgttg tactgagtgg tgaccggaat gacgtgtaat cgtaaacgct
      481 ggtcacgcga agaccgggaa tttatcgaag ccagtgtcgg aaaaatgacc gtcgaggaaa
      541 tggcgaaaaa actgaaagtt gccccaaccg ccctacaggc acatgccaga aggcacggaa
      601 tatcgttgtg tgcataccgg gtcagtgagc acgacaaata tttatgccgt gaactttata
      661 aagaagggct ggctattcat gtgatagcca aaaagatgga attaagcaat cgtgctgtat
      721 ccggcattgt atacagtgaa tattaattaa caggagcttt atttatggct aaacccgcaa
      781 aacgaatcag gaacgccgca gcggcttatg ttccgcaatc ccgcgatgcc gtggtatgtg
      841 atatccgccg gattggggac ctgcaacgcg aagcagcacg acttgaaacg gaaatgaatg
      901 acgctatcgc tgagattacg gagaaatacg cctcacagat tgcaccgctt aaaaccagta
      961 tcgaaaccct ttcaaaaggc gttcagggat ggtgtgaagc gaaccgtgac gaactgacga
     1021 acggcggcaa ggtgaagacg gcaaacctgg taaccggtga tgtgtcatgg cgtcagcgcc
     1081 caccatcagt aagtattcgc ggtgtggatg cagtgatgga aacactggag cgtcttggcc
     1141 ttcaacgctt tattcgcacg aaacaggaaa tcaacaagga agcgatttta ctggagccga
     1201 aagcagtcgc aggtgtcgcc ggaattacag ttaaatcagg cattgaagat ttttctatta
     1261 ttccgtttga acaggaagcc ggtatttaat accacgacga atatttaatt aattcacttt
     1321 cttttaatta tggcgcgatg cgtcagggga ttgctcgcgc ctgaatcagg accagaggaa
     1381 atgaatatga aagcatatgc ttattttaaa ctgagtgacg cagccgctaa cggttatacg
     1441 attcgctggc tgaaagatgt ttctgttaag cgtgacaaaa tcatctgctc attgcagcgt
     1501 gcgtttggcg cagacggtgt ttcccttgtc agtaacagag ggtatgaacg ggttgaatgt
     1561 atctggatga atcaattagc gccggaatta tggcgtggta aatcagacgg actgtttatt
     1621 ggtggatttc agttttatgg cgttgtaccg gatataacaa caccggaagg ccgaaacaat
     1681 gcgacgctta ttcaggaaca tgaagagcaa ctccggaaat atcccacatt cccggtctgg
     1741 ttgtgtaatg aactcggggt tgatgttgta cccaacatgt ttgatctcaa atcagtctgg
     1801 acgatgcacc acagccgtga cggattcggc attttgtttg aggtgtgcct gctggatggt
     1861 gaggttaagg gaccggttcc tgccgaatgc cagcgcatta aacattctga atttgtggca
     1921 ctgacggagg aataaccggt gattgatgca aaagttctga acggtgtcag cacattatta
     1981 cgggcttacg ggcgtctgac ctgcggggtt ctggctgaaa aaatgaatat gctgccctcg
     2041 tcaatggtgt atttcctgcg tgatgcggtt gatgccggag tgctcaccga atgcaacgga
     2101 ttttatgacg ttccgcgccc tcgtctgacg tcacctgtaa ggcgaaacac aacggagctg
     2161 ccaggggttg atgatgctgc atggtgcacg ttccgtcgtt ctttgccttg gctggaaggt
     2221 aacgccattc cggcactggc aaaagaattt gcgacaggcg tactgacctg cgagtcggtt
     2281 tacatcgttg ctgaagtgga tgacgagatg tgcaaacagg gaatgccccg ttttgtgatg
     2341 gcgtatatcg atatccggct gggtcgtttt atttgtggct ccagtggctg gaatatcacc
     2401 gaccacgtcc tgcgctatct cattcttgat tattcaccgg ttccggcgga aacacaggaa
     2461 atcagcgggc atgattaagt caattggctt tgtcctgctg gttggcacct gtgggaatga
     2521 tgcctgcgat gccatacctg taacagaaaa aatctggccc acagagcagg catgtatgca
     2581 ggtgatggag cgcatacaaa aacgttatcc cggtgaaatc ttttattgtg aggatgcatt
     2641 aagaaatgag taaacgtatt tatgacgacc cgaaatatcg ggctgaaatg cgggcgcaac
     2701 tggttgtcag tggatatacg ccagagcagg ccgatgcaac actgacggaa ctgatgggtg
     2761 agcgtccatt tgatgaagct gaatatgaat actggctgaa gcagttcagg ctggaaattc
     2821 tggaaaacac cacactgcca tcgctgataa gaataataaa aagcgttatc gacaatgcct
     2881 gctacaacgg tgattgcctg gctgcagcaa ttgcgaccca tgctctggtg aatctgctgg
     2941 aggttaccgg cgcagttttc ctgaatccag aaaaggtatt aagagatgaa aatacagatg
     3001 attactgatg ctgatttaat ggctgcaacc ttaatttcca gtggcatgtc agaacaaaag
     3061 gtaaatgatt ttctggataa tgtcgtatac caacgctatg agcaggccag agaaaattta
     3121 atttcagaaa aacgcacgat gattgccgaa tcattaagtc cagcctggat tattgaggaa
     3181 ttacgaaaaa tcattgccag ccaggataaa gtagcggcag ttgccgccct cgatgtgcta
     3241 catcgaatga gtaaagagct attcaacacc gaattaattt taaatccggg gggacataat
     3301 gagccgcaca tccctgatta aattaattca tgttgcccgt cgtgacctgc aactcgacga
     3361 tgacacttac cgcacctttc tggtgcagtg cacgggcaaa accagttgcc gcgagctttc
     3421 cgttgcacaa ctggaacgtg tgctcgatgc catgaaagag cgcggtttta agaagcagaa
     3481 aaaacatccc cgccgtcgct ttaaggggca tgtcacaccg cgcgagaaga tttataaaat
     3541 ctggcagcag atgttccttg atggtttcgt ctccgatatc agcgacgctg cgctggacaa
     3601 atacgttgaa cgcctgacaa ccagacgtaa tggcggtcag ggtgtttcca cgctggcctg
     3661 gtgtcacggg gaatcgttgc aggttgtgct cgaaacgctc aaacagtggc atatgcgctg
     3721 catccgtgaa gcctttgccc gacatggtgt gcctttacct gtgagcaaaa ccgggcagga
     3781 gctgcgcggc tatgatgcac tgaccagtgc gtatgcccgc gcacgaaaca gcgggagaat
     3841 tgctgtatga aacagatgaa agagcaggat ttattcgaag acttccggga cgacagcgtt
     3901 ctggaatacc ttaacgatta ccaggaaaac acggcttatc cggcgttact ttctgaactg
     3961 aacgcactgc tgcgtaaaga gctggcccgg attggcgcag accctgctca ttcccttgag
     4021 ctggttgtgg cgatttgtcg ccatatcggc gggatgcagg tttatgttcc gagggggaat
     4081 attctggaga atcttgtccg ggatatgcgt atctggcgtg attttaacgg ccataatatt
     4141 cctgaactgg ttcagcgtta cggggtgacg tataaaaccg tctacaaggc catcaaacgg
     4201 atgcggcgtc tggagtgtaa taaataccag ccggatttgt tttaccactc cccgaacctg
     4261 cctcaccccg ttcgtcgtcc gccacgacgt ccttaatcaa tgaagccggt aaatccggct
     4321 ttttttatgc ctccggcacc atgaagcaga ccacgtttaa ctctgcttca caggtgcttt
     4381 tatggaaaat ctgaaattct ccccggcctt tgaacatgcg ctgaatttta ttctgcgtcc
     4441 cgatatcgaa ggcgtctatg tcaatgaccc cactgaccgt ggcggcgaaa ccagatacgg
     4501 catttctgac cgccgcgacg gtgtgattga cggcaaaacc gatgtcagcg gcgacggtaa
     4561 accggatacc cgcatcaggg atttaacccg cgaacaggtc gcgcagattt actggcgcga
     4621 ttactggctg cctgccggat gtgaccagtg gcctgatggc gtggcgatgt ttgtgttcga
     4681 tgcggcggtt cagcatggcg ttaaaaaagc tatcaggatt ttgcaggaag ccgctgatgt
     4741 ggatgctgac ggcatcatcg gtccgcgtac ccgtaaggca gtgagcctgt ccaccccgga
     4801 ctggttgctg acccgctgca ttgtccgtcg ttcccgcttt tatgccgaca tcatcaaatc
     4861 caaacctgcc cagggcaaat acctgaacgg ctggtttaac cgcatggaga aagtgaccga
     4921 cgcctgtctg gaaattatcg acaacgtgtc gtccgtcacg cggggatgat atgggcaaag
     4981 gctgggatgc ctcgctgaga gccgggcggc gtgaccgcct gcgtcaggag gttttacatc
     5041 gtatggccgg aggtccgccc ccgaaaccgc tggactatac cggtcatgac ggcacacacg
     5101 ccagccacta catgcgtggc tggaacaccg tggatacacg ggacattttc tggcagtgcc
     5161 agaagtacaa ggaaaaactc aatgtacaga atgaatagtc tttttgttcg cgttatttac
     5221 tcccgcttta tgttgtgcca gtggtcgttt ctggggctgt cggcagtcct ttttctggcg
     5281 agctgttttg ccggatggca ggcgtttgca gtcatggtgc tggcatacgg cgcgacactc
     5341 tgcctgattc acagtttcca gctgggcaac gtaccttatc gcctgcaacc caaaaggcgt
     5401 ttccgggccg cctcccttcg aattgtcgtg tggtcatggg gggtctgggc cgttggtttt
     5461 ttcatgctga cattcggcct tctttatctg aaggagcctt atcagctggc gttctggctg
     5521 ggcggtatct tttgtgcggc actttataaa catcagcgcc gttttcataa gggggattca
     5581 tggacccgtt aaccctttca ggcatcgcct ccgttctgct gaaagccggg ccggggctga
     5641 ttcgttccgt cgggcgctgg tttggtggca gcacatctgc cgccgctgac tcggtggccg
     5701 gtatggtgga aagcgtccgg gaaagcctgc cggtggctga acagcaacgc attctggaac
     5761 aaaaactggt gatgctgtca ccggagcaac tggtgcagct ggaaaccctg aaagtccagc
     5821 tgcaacagct ggaggtggag cggcagaagc tggtgctggc tgaccagcag gccgctcacc
     5881 acgaacaaca ggaaaccatc cgcaacggtg acaacgccac ggacagctat gtgcgccaga
     5941 cgcgcccgtt gctggcccgt ctgtcctgtt acagcagtct ggcctatgtg ctgttgctct
     6001 cctgtggcca gattgccggt gcggttgccg gtgcgaacgg tatcacgctg catatgccgt
     6061 caccggactg ggatatcacg ctgatgttgt ttaccccggc gctgagctat ctcggggtaa
     6121 gaacccttga cggctttgcc cgttacagca aatcgagctg tcacaaaata tcagcggggc
     6181 cgaaatgacc gatgaaaccg accgcatcag tgaagtggtt ttaaacgaac gtcaggacgt
     6241 cgttaacgcc tggctgatgc ggataaaaga agcaccccac agtcggggct tctgcaatga
     6301 ctgcggtaac gttattccgg cacagcgcat tgcggtgctg ccggatgtgg tgacctgtat
     6361 tgactgccag caggcgcggg aacgcaggag gaagacgtgt cctgggaagt gatccgggcc
     6421 aactggcctg ttttatgggc gctgttgatg acagcaataa acctgattca gctgattctg
     6481 gcgaagactt acgtcaaacg cgaagagttt gattcgctgc gttcccgcgt gtcggtgatg
     6541 gaaggcctgg tgaatcagct acccgaccgc aatgaatttc accgcctgca actggatatc
     6601 agcaacctgc ggggtgaaat caaagagctg gggccatcca tccgccaggt atcccgcatc
     6661 agcgatttgt tattagagaa cgaattaaag gaaaaaaatt aatggcgatg aaagagatcc
     6721 tcactgaaga ccgtcgtctg gtgttgctgc gttccctgct ggactgtggc gacagcgcga
     6781 atgaatccgt gttgcagacc tgccttcagg cctacggtca taaggtatcc cgtgatgtgg
     6841 tgcgcaccca gcttgcatgg ctgcgcgagc aggggctggt tcgtctgtct gatgtgggcg
     6901 gctgttacgt ggctgaaatc accggcagcg gcgaggatgt ggcaaacggc ctgtccggtg
     6961 tgccgggcgt taaaaaaccc cgtgcgaggg actgaacatg gcccgaaaac tgaagccgtt
     7021 aagtcgtgga gaacgggccg tcgtacggca gctggcgtac tgccttgtgc tggcagacat
     7081 tgagcaaaac gccattgtcc gtgcatacga aaaacataca ggaaagccct ggaacccgga
     7141 tgcaccggat acccccgtga aacgtgccct gcgttcatca ccggcatatg cgcggctgtg
     7201 gaagctgcca ggtaaggata ttcagtccgt tcgtgaagaa atatacgctg gtctgaaaac
     7261 acagaggaca gaagatggaa aatgaacaac gccccacccg tggccgtctt tccaaagtgg
     7321 atttactccc ggacagcatc cgtgagcagt tacaccagat gctgcgggaa aagcggcaca
     7381 cgcaggaaga aatccgcgaa gccatcaatg ccctgattga cgaacacaac ctgccggggg
     7441 agatgcagtt aagccggacg ggcttaaacc gctatgccag ccgcatggaa aaagtcgggg
     7501 caaaaatccg tgcctcccgc gaaatggccg aagtctgggc ggcaaagctg ggttccgcgc
     7561 cgacgtcaga cgtcggcagg ctgctgatgg agtttgtaaa aacgctggcc tttgaaacgt
     7621 ccatgtcgat gacagaagac gacaaacctg ttgcaccgaa ggcgctgggg caactggcgc
     7681 tggttgccca gcgtctggaa gcggcagcca tgaccagcca taaacgcgaa aaagcaatcc
     7741 gcgatgcgtt tgcgcaggaa atggcagaga aaaccgaaga gctggtcaga gcgggcggtc
     7801 tgtcaggcgg tgcggctgac accatcaaac gtgaaattct ggggattagt gtatgacaca
     7861 gatgaacgca ttcagtgaat ccgatgttct gctgccgtat cagaaacgct gggtggcgga
     7921 tgattcaaaa ctgaaaatcg ccgaaaaatc ccgccgtacc ggtttaacct gggcggaagc
     7981 ggcagatgcg gcactgaccg catcactgaa gaaggaaaac ggcgggcgcg atcactttta
     8041 catcggttcg aacaaggaga tggcccgcga atttatcgac gccgtggcga tgtgggcaaa
     8101 agcgtttaat gcagccgcgg aagaaatccg cgaggaagtg atcaccgacg aagacaagga
     8161 cattctgacg ttcgtcgtgt acttcgccag cggatttaag gtcaaggcgc tgtccagtaa
     8221 tccgggcaac attcgcggga tgcaggggaa tgtcaccatc gacgaagcgg cgtttcatga
     8281 aaagctggat gaggtactca aggcggtcac accactgacc acgtggggcg gtaaggttcg
     8341 ccttatctcc acccatgacg gcgtggacaa cccgtttaac cagctgattc aggaaagccg
     8401 cgcgggcaaa aaagattacc gtattcacac catcacgctg gatgacgcct gtaatgacgg
     8461 gctgtaccgg cgtatctgtc aggtgcgcgg catggtgtgg tcaccggaag ccgaggtgga
     8521 atggaaagaa ggcctgctgc gaaataccgc cacccgcgaa gatgcgctgg aggagtatta
     8581 ctgcgtcccg aaaaacggcg gcggcacgta tatcccccgc tcactgcgtg aacgtgcggc
     8641 ccgtggcacc ggaaaagtgt tgcgctttac cggcacaccg gaatttaacg cactgacgga
     8701 aggccagcgc cgggcagaga tcagggaatg gctggaaacg gtggtgcgcc ctgagctgga
     8761 aaaactcccg aagaatctgc gccactgtct gggggaagac tttgcgcgtt cgggtgacct
     8821 gaccgtgctg gccccggtgg cggtgaacga cgacaccacc cgcgaggtgc cgtttctggt
     8881 tgagcttgcc aatgtgccgt ttaaacagca ggagcaggtg ctgttctggc tttgcgatcg
     8941 tctgccccgt cgtgacggta tcaaaatgga tgcgcggggt aatggtcagt atctggcaga
     9001 acaggcggca gaacgatacg gcgacgaggt ggaacaggtg atgctgtccg tggcgttcta
     9061 ccgcgaaaac atgccccgtt tccgtgcggc gtttgaagat gatgagctga tccttccgaa
     9121 gcatgaagat gtgatcaatg acctcggggc cattcagtta ctgcgtggcg ttcccggtat
     9181 tgacgatgcc cgtactaaag gcagtgatgg ccgcaagcgt cacggcgacg ccgctgtggc
     9241 tattttcctg ggcttccttg ccagtaaaga tgactgccac cgttacgagc tgcaccgcct
     9301 gaaccgcccg gcgaaaccgg aagaaggcaa cgcgcgccgc cagatgaagc tgacacgcgg
     9361 cctgaaaaat gagggaggtt tactgtgaac ctgaaacaac tggccggggc cgttcgccgt
     9421 ctgctgaatc cggcaaccgg tgaggaagac acgctgaaaa aagaggcgct caacgaagca
     9481 caggcccgtc cgcgtcatgc cggtgtgcgt ccgacctcgc cgggtatcag cgtcgcctcc
     9541 gggctgaacc ccggcaggct ggcaggcatt ctgcgcaatg ccgccgacgg catcacgcgt
     9601 gattttttta tccttgccga agagatggaa gaacgtgatt tgcattacgc ctcggtgctg
     9661 cgcacccgca agctgacagt ggcaggtatc gaaccggtgg tggtggccgc cagtgatgac
     9721 gatgccgacg tgcagctggc ggacgccatc cgtgcgctga tggaagcccc gcaaatcccc
     9781 gaactgatgt ttgatttgct cgacgggctg ggaaaaggcg tggcggtctg tgaaatcctg
     9841 tgggacaccc gcaacaacca ctggatgccc cgtgattatg agtgggttga tccccgtttt
     9901 ctgaaagccg aaaagcccac gctgcgccag ttccgcctgc tgacggacga tgaaccggtg
     9961 gatggcgtac cgctgacacc gggaaaattc attgttcacc agccgcgtct gaaatccggt
    10021 ctgccgctgc gtaacggtct ggcccgtctg gtggctgtga tgtacatgct gaaatccttt
    10081 accgtgcgcg actggtgggc atttgcggaa aaattcggca ttccggttac ggtggggaaa
    10141 tacggtccca acgccacgga ggagcaaatc cgcgtgctga ttgatgcgat tgcctccatt
    10201 gcctcggatg ccgggtgtgc cattccgcag tccatgcaac tggagatgca ggaaaccgcc
    10261 agccggaata acggcggcgc actgttccgt gaaatggcgg aatggtgcga tgcgcaaatc
    10321 agtaaggctg tgctggggca gaccatgacc acggataacg gcagttcgcg ttcacaggcg
    10381 gacgtgcaca atcaggtgcg catggacatt gtgcgctggg acgcgcgaca gctggctaac
    10441 acgctgaatg aatatctggt gcggccctac atcgaggcca actacgggcc acaggcgcac
    10501 tacccccgtg ttgttctgcg tatcagtgaa gccgaagatc tgaaggcgct gacggatgct
    10561 ctggtgccgc tgattgaccg ggggatgcgg gttcaggaat cggagctgcg ggaccggttc
    10621 ggcctggctg agccagacga aggggctgat gttctgcatc cggtttccgc cgggatgccg
    10681 ggtgatatgg cgatgaaccg cgagcgcgtc gccctgaaca gggaacaccc cgacgaactg
    10741 gcacagatgg tggatgatgc cctgcgtgac tggcagaaaa ccggcgaggc gttcacgaac
    10801 ccggtgctga cactggcgca ggagtgcgac agttttgatg actttctgaa acgcctgcct
    10861 gagcttcagg aaacgctgaa cgcggacgac tttgccctgc aactggcgga ggtgtgcttt
    10921 aaggcgcgtg cgctgggaga cactgctcat gcgtgaaacc ctcatcccga aagaggcgct
    10981 ggcgtggctg aaggcgaaga agctgcgccc cggttttgat taccgggatg tatggcggga
    11041 agagcaccgg aacagcttca cggtggcaaa aatgctgcaa ctggatttgt tgtcggatgt
    11101 gaaagccctt gtggaagacg ccctgcaaag cgggcagacg ttcagcgagt tccgggagat
    11161 gctgcaaccg ttgctgataa aacgcggatg gtggggcgta caggagatgg atgatccgct
    11221 gacgggtgaa acccgcaccg tgcaactggg cagcgaccgc cgcctccgca cgatttttga
    11281 taccaacatg cgcaccgccc gcgcggcggg ccagtgggaa cgcattcagc ggacaaagca
    11341 ggccatgccg tatctgatgt acgagctggg gccatcccgt gagcaccggg tggagcatgt
    11401 gaaatgggcg cgtctgtgtc tgccggtaga tcatccgttc tggcagacgc attttgcccc
    11461 taacggctgg ggctgcaaat gcaccatccg tcaggtcagc cgtggtgagt atgcgcaact
    11521 ggcggcacag ggtaccattc acaccgaagc gccggaaatc agaactgtcc gctgggtgaa
    11581 caaacgcacg ggcgaagagg aagatgtgcc ggaagggatt gatccgggct ggaactacaa
    11641 tcccggtata aaccgtgagc aggagctggc gcgccagctg gcggccagac aggcccgttt
    11701 taacagtgaa taacccaccc cgccgtaaat acccctgaaa cgcatcagaa acgcgttttt
    11761 tattctgatg gcatgaatgt acaccctggc ttttttgaag cgtctgtggc gtttttgaag
    11821 gggttttgaa gggggtattt ccccgttttc agtgaagccg gtaaatccgg ctttttttct
    11881 gccttccgca tactgaccgt cggtaacccc agacgacgga gactgacatg caaccggaac
    11941 tgctggcgct gtgtttttcc ctgccagaac ccatccctga gctgacaccc gctcaactgc
    12001 cggaatggct tgaactcgtt cctgcgggtg agttcaccgg gcgcgatggc cggacgtgga
    12061 tgaaccgcaa tccacatgag gtggttgccc gttcgtccga catcaacatt ccggtggaca
    12121 ttgaacatgc caccgaaatt aaaggcccgc ggggtgaaga ggctccggcg tatggctggg
    12181 tggaagcact gcgggtgacg gacagcggca ccattgaagg gcgtgttgtc tggagtgagt
    12241 ccgcccggtg gatgctgagc gagcgccgct accgctatta cagcccggcg tttttccatg
    12301 acgcagacgg tgcggtgacg cgcctgtcca gcgtcgggct gaccaacaaa cctaacctgg
    12361 attttcctgc actgaatacg gagaaaaacc cgatgacagt acctgtgcaa atcaccggcc
    12421 tgcttgggct ggctgaatcc gccacggtgg acgataccgt ggccgccatt aaacaacttc
    12481 aggagaacga acaggtggcg ctgaaccgcg cacagacacc tgacctgacg aagtttgtgc
    12541 cggtggaaac ccacaatctg gcactgaacc gtgccgaaac cgcagaaaaa cgccttcagc
    12601 aactggaaga gaaggaagcc gaggcgcttg tggatgcggc catcggggcc ggaaaagtcg
    12661 ccccggcaaa ccgtgacatg ttccttgcca cctgccgcac ggaagaaggc cgcaaacagt
    12721 ttgcggagta caccaaaggt gcacagccgc tggttaacaa cgacaagccc agccagggca
    12781 aggataaacc cgcgcagaca ctgaccgatg ccgaactggc gatgtgtcgc agcatgggta
    12841 ttaccgggga agagttcctc gccgctaaac cgaaacagga gtaagtaaca catgtcacag
    12901 actatcagtt ctgaaatcct tcatgcactg accacctgcc tgagcgccgc ctttacccgt
    12961 ggactgagtg gtgtcgaacc gcagtggaat cgtattgctt ctgaggtgcc gagttcgtcc
    13021 gcctccaaca cttacggctg gatgaaagat ttaccggaca tcaaagaatg ggtcggtgag
    13081 cgtcagctgg caacgctgga cggttatggt tacaccatca ccaacaaact ctgggaaagt
    13141 tccattcgcg ttaagcgtga acacattgaa gatgaccaga ttggtcagta cagcattacc
    13201 gccgaacgct atggccgtat gactgctgtg ttcccggaca agctgtgtta cgccctgttg
    13261 tgtgccggtt ttaacaccct gtgctttgac ggccagaact tctttgatga agaccacccg
    13321 ctgggtgacg gtacgtacag caacgttgtc ggcaccccgg catcagacaa gggcgaaccg
    13381 tggttcctga ttgatgagtc gcaggtgctg aaacccatca tctggcagac gcgacgcgcc
    13441 tttaagtttg aagccctgga cgatctgaac agcgagcaca ccttcaagaa ccacgagttc
    13501 ctgtacggcg tggacggtcg ctgtaatgcg ggcttcggct tctggcagac cgccgtcggt
    13561 tcccatgcag cactgacggt ggagaactac aaaaaagcca atgagttgct gcggggcatg
    13621 aagggcacca acggtgagcc gctgggcatc cgcccgacca cccttgtggt ggggccgaaa
    13681 aaccgtgcgg acgcgaagct cattattgac gccatgctgg ttaacggcgg cgattccaac
    13741 atctggtaca aggatgtgaa gatcgtggac agcccgtaca tcaccacccc ggcataaccc
    13801 gtcatccgta aaccgcagtt aaaaggtgct gtgaatgccc ttttaactgc cttttaaaag
    13861 gcagagtcat gagtgaaaaa gcagaaacca aaggcgcgaa agccgcaaag aacagcgctg
    13921 cacaggaaaa cccggcaccg ctggcagacg ttattgtggc tgacggtcag gcgaatgaac
    13981 cacgtccggc tgaagacccg gttgctgtac agggtgacgc ccctgtccgg ctgaacgtca
    14041 gggccgtgtc tgaaaacggg ttctggcgct gtggccgttt ctggtcacat atcggtgagg
    14101 atgtggcggt gagcgctgcg gttgccaccc gcctgatggc agagccgaat ctgattgtcc
    14161 gggaagcgga gaaaggctga tgggatacat cacgcaggaa gacctgttac gcgcggacgg
    14221 caatcttgtc tggaacatgg cgattaaccg ggaaaccaac gggctggatg aagacaaaat
    14281 ccgtcaggcc atcagtgatg cggaagcgga aattgattcg tttctgtccc gccgctacca
    14341 gctgccgctg ggggtgacgg aaatcccgcg cccgctgcaa cgcgtggcgg tatcgctggc
    14401 gttttactgg ttatcagagc gtgacaatca aatcacggag ctgatccaga aacgctacga
    14461 cgacgccatt aaaaccctgc gtgaaatggc gaacggcacc cgtgacctgg gcctgccgac
    14521 gtatgccacc ccggcagaaa ccgacaacgg gaaaattatt gtggtgggtg ccaatgcccg
    14581 gctgttcacc cgtaacaacc tgaaaggggt gctgtgatgg ggatttctgt acaggtcagc
    14641 ggtgaccagc gtctggagga tatccgccgc gccgttgaaa agctggcaga gggttcattg
    14701 cagtcagagc tgctggaaag catcggtgcg gtggtggaat cacagacccg ccgccgcatc
    14761 atcgatgaga aaaccagtcc gggtggcgaa cgctggccgg actggtctga cgggtacaaa
    14821 aagacccgcc acggcaacca gagtctgctg cgcggtgaag gccatctgct ggagagtatc
    14881 cagtacatcg tggaaaaccg cgtggtgcgt atcggttcac cgctggatta tgcccgcatc
    14941 atgaatgacg gcttttccgg cagcgtgccg gtcagcgccc acaaacggct tatctcgcag
    15001 tgtttcgggc gggcgctgaa atatccggtc tggcagaccg tcggtgcaca tcaccgcatg
    15061 atgaacattc cgcaacgtga atttctgggg ctgtcttctg ccagccagca ggagctgcaa
    15121 caggttatca gtcatttctg gaaggaggtt ctgccatgac agaacaacgc cctgaactgc
    15181 gcacaccggg gagcaccgtt gcggccgcag agcgcattgt agcctggctg cagacggccc
    15241 ttcaggggga cacccccgac cgggctgatg tggtggagcg tcacatcggc cagttcaaca
    15301 gcacggatga agtgaaacgc tatctgtccg gtcgcaccgg ctgtatccgg gtagctgccc
    15361 tgcgtgtccg ggatatcaac ccgcgcggcg ggctgtccgg tctggttacc tgggtggctt
    15421 acatcatggc gacggattcg tgggggtatt cccgcgacgt gcgctgtgaa gtgctggccg
    15481 ggaaggtgat caaacgcctg ctgtcgtcgg atgccaccgg gggcatgggg gctgaacgta
    15541 tggccgctga tgtgcgggca gacaacattt actccgtcag cctcgacggg ctgggtgtca
    15601 ccatgtgggc ggtgacttgg gagcaggaat tccggctgga tgaagagatt gatctcgccg
    15661 cgctcccgga tttcctgcgc ctcggggcga cactgcgcag cggcgaacac actgaaatta
    15721 acgacgtgat ccatgtacgg ggtgacgatg gaacagaaac tgattaagcc agcgcgggaa
    15781 aacgtccgtg tccgtaaacc ggatggcgca catttatccc cggaagggga acgtctcaac
    15841 gtctgcgctt actggctgcg ccgtgaagcc gagggagatg tggaaataac cacacttccg
    15901 aaaaataaca acaaaaccag agggaaaaaa taatgtcgct gggttccatt cctgatgata
    15961 tccgcgtccc gcttgtctgg atcgatatcg acaattcaca ggcgctggag ggtgcatccg
    16021 cgcaaagccg aaaaattctg gtcatgggcc atgcggtatc gtccggcagc gcagacgccc
    16081 tgtcactgac ccgtatcacc agtgacagcc aggcagacca gctttatggc aaagggtcga
    16141 tgctggctga aatgctcaaa atgctgcgtc gtgccaacac gtacacggaa acctgggcaa
    16201 tgccggttgc cgcgcctgaa ggcgctgccg caaaagccac gctgaccgtg ctgggtacag
    16261 cgactgaagc cggaacggtg gcactgctga ttaatggtgt gtccgttcag gtgagtgtga
    16321 gcgccggggc tacgaaggaa aacattgcaa aagccattgc tgatgcggtg acgaaaaagc
    16381 ctgccacgca ggtggccgct gcggtgaagg atgatgccac ggataccgtg gagctgaccg
    16441 tgaactggca cggcgtcacc ggcaacggtg ccgacgttcg cctgaactac cacaccggtg
    16501 aagccttccc ggcaggtgtg aaggtgaccg caaccgcgtt taccggcggc accgggacac
    16561 cggaaatggc agacgccgtt gcggccatcg gcccggagtg gtttaccgat atcatcgccc
    16621 cgttcaccga cacgaaaagc ctgaacaccc tgcgtgatga actgctgaac cgctgggggc
    16681 cgctcaaaat gatggaagcg cagctgtgga cggcgtttcg tggcacacac ggggaaaccg
    16741 gcacgtttgg tgaaacccgc aatgactggc tgattagctg tatcggcacc aacctgtcac
    16801 cgcacccggc gtggatgtgg gccgcgtcat acggcgcaac ggcagcgtat caccttgcca
    16861 ttgacccggc gcgaccgctt cagacgctgg tcctgaccgg cattctgccg cctgcgcgta
    16921 atgttcgctg ggatatgccg gagcgtaatc tgctgctgca tgacggcatt gccacgcaca
    16981 tggtggacgc cggggataac gtctgcatcg agcgcgaaat caccatgtac cgggttaacc
    17041 agtacggtga tgcggatgtg tcgtaccttg atgtgcagtc acccgccacg ctgggccgta
    17101 tccgttacat catcaagaac cgtttcacga accgttatcc gcgccacaag ctggcggatg
    17161 atgacgtgct ggactcgctg gatgcggggc agccggtgat gacgccgaag ctgtgcaccg
    17221 cagaactgct ggatatctgc cagaccgaac ttctcccggc gggccttgtg gagaacttca
    17281 gcgattacaa agacacgctt caggtgacac gcgacagcag cgataaaaac cgtctgaact
    17341 ttatctgcca tccgaatctg gtgaaccagt tgcgtgtact ggcaggcctg attcagttca
    17401 agctttaagg gggccacatg gcaaaaattc ttggcatggc gaccattcgc gtgaatggcc
    17461 gcgaaattaa aaccgagggg aaatccacgc tgaatccggg cggcttcagc cgcacccagc
    17521 atatgggcgg cggcaaggtc tggggtatct ccagcaagat ggcctcgccg tccatcaagg
    17581 tgaccattgc ggcggcagcg gatatggacg tgattgaaat cagcagctgg gaagatgtca
    17641 cggtgatgtt ctacggcaac aacggcctga actacatgat gacgggttca gccacggata
    17701 acccggcgga gctggacgag gattccggca ccatcagtgc gaactttatc ggtgaaaaat
    17761 gcgtaaaggt gtgacatggc cgcgatggaa tttgaactga aacacgggct tctgaccggc
    17821 aaaggcacgg cagatgaaac cctgcataaa accgtgaaac tgcgcgaact gaccgccagt
    17881 gacgtgattg atgcgcaact ggccgcagaa cgcgtcgtca tgggcgggaa cggaaaggcg
    17941 gtggcctact gctcggaagt gctgatgggg ctggagatga tgcgccgtca ggttgcgtca
    18001 atcggtaata tccccggccc gctggacatg aaacagctgc gaatgctcca cccggcagac
    18061 cttgaactta tcagcacgaa agccgctgcg ctggatgaaa tgcttgagga ggtggcaacg
    18121 cgggggcgaa ctgatgccgc tggcggcggc tctgatgaac ctgctggtta acctgtcttc
    18181 gcgattcagc attcagtacc tggaacagct gcccctgcgg cagctgttcc gcctgataaa
    18241 gcaactggag aaacagcatg gcaacaggta accgtctcag cacggaaatc atgatcaacc
    18301 ttgccgggaa cctgaccgcc aaagcccggc agtacggcgc aaacatgtcg cagttcgccc
    18361 ggaacaacca gaaggccatg cgccttgtca aagccacaac ggaggccgca ggacgcggcc
    18421 ttgatacgct tggtaaccgc tacacggcaa tgatagccgg gcttggcagt agtcttacca
    18481 tcaaacaggt tgccgatttt gatgcgcaga tgcgccggat gggtacggat gcccagttaa
    18541 ccactgaaca ggtaaagacg cttcacgaca aaatcaggga tgtatcgaac cagaaagata
    18601 ttcgtattga tgcttctgcg cttggtcagg gggccagcga acttctgggg aaaaccggtg
    18661 attatcagta tgtggtggac aacctccgca acatggggtt gatgatgcag gcgttcggtg
    18721 ttgacggaca ggttgcagcc gggctgatgg cccagttctg ggaaaaaaac gtccggggtg
    18781 ctgatgcggt caataacatg atggacaggc tgtattcgca gttcgcagtg gggtctgtaa
    18841 gcgttgccga tgttgcccgc gctgccccga aactgttttc catcattcag gatcagggac
    18901 cggaagccat tgcccagatg ggggcatttg ctcaggtgtt cgccaaaaac aaaggcagca
    18961 ttgatgagac ggtaaccagt attcaggcca tgtatgcttc cctgtcggac aaaaagaaca
    19021 tcgagttcct gaaaaaaaac ggcattgacg tttttgtcaa aggcacaaaa gacatcaaaa
    19081 agccctatga gctgatgatg gaaatcctta aacgggccaa atacgacccg ctgaaacttc
    19141 aggatgtgtt tgatctgacc ggaatgcagg ggattaaggc gctgcttaac cctgaaaacc
    19201 ggcaactgat ggaacaaatg atttacggca cgattgagct gggttccacg caaaaggccg
    19261 cacagaccaa tgccgaaggc tttaatgccg caatacagtc actgaataac gaatggcaac
    19321 gttttgctga agggcaactg gcaaagcccg ttcaggacct ggcggatgcg ctcaacagcg
    19381 tggaccagaa cactgcccag aactggttgc aggtcggtaa atatatggcg atcgcgctgg
    19441 gcggcattat cgccatcaga aagacgtatc agttcggtaa aaccatccac gacatcatga
    19501 atcccaaagg gaaaggcaaa gggatacccg gcagcattac ggatgttttc ggctccggtg
    19561 tgatgccggt ttatgtggtc aatatgggca gtggcgggat gaagggtaat accggcggtc
    19621 tgccggatgc gccggattca tcgcgcaatc cccgtaactc ccgcaaccca cgtgggccaa
    19681 agacgccagc gctggttaca ccacaaactg catcaggatt taatctcctt gacatggtgc
    19741 tctctgcttt ccctaagaac aaagaggaag cagatgcgtt aatcgcacgg gttcaggaaa
    19801 acaacaaccg cccgacggta tggacggata tcaggaactg gtttaactct ctggaaaaaa
    19861 agaatattgc cgacccgcga ccgtgggctg gtatgcaacc cactcagaat tatcctttcc
    19921 ttccgcaaca attgcagggg gaaatccgtg tggtggtgga aggtgatgcc cgcgtgaaaa
    19981 gcgtcagagt ggatcaaccc ggcgtcagac tcagtgcgca ggctggcgtc accagcgtgg
    20041 agcagggtta atgacaacga gcaacggtaa atgggacggg ctgcgcgacg cctcgtttcg
    20101 tggcgtcccc ttctttctgg tggatacgga gggcaccggt ggtcgtcgtg ccattccccg
    20161 tgcttatccc cggcgtgaaa ccgcctggac ggatgataac ggggccgttc cggggcaaca
    20221 gcagattaac gcaaagctgc caggtaaaaa cttccgggat gatttaaacg cccttttaga
    20281 tgcgctcaat acccccggcc ccggcgagct tgtccacccg tggttcggga tacagaccgt
    20341 acaggttggc aaggtcaccc atcgcctcag cacggaggaa gacggcattg cgtatgtcac
    20401 ctttgaagtg tttgaggcag gcgagcgcct gttcccgtct gcggcggata acacgcagca
    20461 ggaagtgctg acaggcattg atgcggtgaa agccgccatt gacgatggcg actggttcgg
    20521 cgcactcgac gggctgggtg acatggccga cagttttctg gccgatatgg aaaaccttgt
    20581 ggctaacctg cccacgttac cggcagcact gaatcagtgg atggacaggc tgaagcattt
    20641 taaggcgatg gccggaaccc tcattgccac accggggcgt ctggtcagtg agctgtcagc
    20701 acgggtggaa gatattgcgg gccttgtgac ggaaccaccc gaagcactgg cggtttacac
    20761 gacattacgc aaccagtggg ccggagaacg tgccaggcag attgccaccg gcgcactgcc
    20821 ggaagatatc accgtgaagc ccggcagcgt ggcagacggc aaccccggct ttgccatcgg
    20881 gctgtcaccg gattatcagc cggtatctga cagcctgcaa aagaacattg acgacttccg
    20941 ccaggtggtt gtgctgggag ccctgctggg acaggccagt gccgtggcct cgatgacgtt
    21001 cgatacccgt gatgcggcgt tatctgccgg tgacacgctg gcggctgaac tgcatgagca
    21061 ggcggtggcg gcggtggaaa acaaccagcg gacattgtgg cgaacgctgc gcgatttacg
    21121 gcaggccgtg attacggatg cccgcgagcg tgccgcccgt ctgccggaaa cccggcaggt
    21181 gacgctgacc acaaccacct ctgccgcatt gctggcatgg cgcgagcatg gtgacacacg
    21241 ccgacgggat gaaatcgtgc agcgtaaccg cctgcgtcat ccgtcattca tcctgccgac
    21301 gcaacctgtg gagatcaccg actgatggaa tccgtgattc ttaccgttga cggcaaactg
    21361 tgggaaggct ggacggaaat gtccgtcagc cgttccctga aggcgattgc cggtgagttt
    21421 gaactcagtg tgacaacccg ctggtcagcg gcggcaccgc gcgtgattcg tgaggggcaa
    21481 ccctgcacgg tcaggctggg cgcggatacc gtgctgacgg ggtatatcga tgattttatt
    21541 cccggctatg acgctgaaaa cgtggagatt cgcgtcatgg ggcgcgataa aaccggcgac
    21601 ctggtggact gctctgtggt gcattcgtcc gggaaatgga aaggcgtgcg gcttgaacag
    21661 gtggcggctg atgtctgccg cccgttcggg ataaccgtca tcacggaaac cccgaccggg
    21721 gaggcatttg cgtctgtcgt tctggaacag ggtgaaacgg gctttgaact gctcgaccgg
    21781 ctggcaaaac agcgcggcgt tctgctgacg tctgacggcg cgggtaacct gattatcacc
    21841 cgcgcctctt ccgttcgcgc gggcgtgtca ctggtgctgg gaaaaaatat cctcgccgcc
    21901 cgtgggcgct tcagctggcg ggagcgtaac agccagtaca tcatcaaggg caccaccagt
    21961 gccggtggca aactgtggga cagccagcct gccccgatgg tgggcggtcg ccagtacatc
    22021 accgaagacc cggaaattaa ccgttaccgc ccgcgcattc tggtcaatga agacagcctg
    22081 accgtgggcg gggccagcat tcgcggggaa tggtacaaag cccggatgct gggagagtcc
    22141 cgcacgacgg aaatcacggt ggcaggctgg cgcgaacagg gcgacagcgg cccgctgtgg
    22201 cagaccaacc gccttgtcga tatcgacgac agcattcaga acctgaaaac cacctggctg
    22261 atatccgggg tgacatggac ggatggcgca caggggagga tgacggttct ggcgctggtt
    22321 ccgcctgaat cactggacat gccggaaatg aaagcgaaga cgaaaaaaac aaaggcggtg
    22381 gccacatggg attaaacgca attgcccgcc gcctgcgact gatggttgac cgcgctgttg
    22441 tccgcctggt gtctgacggt ctggcacggc agaacctgca aattcagacg ctggcagatg
    22501 ccaccgatga cgacgtggaa cgcttccaga actacgggtt tacgtccgtt ccgccggaag
    22561 gttctgaagc cattgtgctg gccgttggcg gacggcgtgc cgggctggtt gccatcgcgg
    22621 tggaagacaa gcgatgtcgc ccgaaaggac tgtcccccgg cgatgtcagg gtgtatcacc
    22681 gggacggcaa atcacacatc accctgaagg aaaacggcgt cattgaaatc acaggagaac
    22741 aggtagacgt ttcagggaaa acggtaaatc tcaccgccga cgaattgctg aatattatcg
    22801 gtaaacaaat gaaattcgtc ggtccctgtg aatttactga agatgtcaga atcaagggta
    22861 aatcattcag cgaacacatt cataaggacg gcgacaatga gaagacatca ccgcccgtat
    22921 gacgacagaa atccgctgga ataacatgct ctcacggggt gacatcaccg tcacccataa
    22981 cggcctctca cgggatgagg ggctggtcac tcaggttctt atctgccttt tcaccgatgc
    23041 ccgcgctgat gacgatgatg tcattcctga cggctccggc gacccgcgcg gctggccggg
    23101 cgacacgtac agcgattttt cgtggggttc ccgcctgtgg ctgcttgagc gtgaaaagct
    23161 gacggaagat gtccgcctgc gcgtggaaga ttacgcgcgg ctgtccatgc agccgcttct
    23221 gcgtgccggt tacgcccgta acgccaccgt cacggccagc atcatcattc ccgaccgtat
    23281 tgcctttctg gtggtgttaa cccgcccgga caaaaccaca ctcagaatcg aaatcacccg
    23341 tcgatgggag gccaccatta atgccgtatg aaatccccac gctgggcaaa ctgattgccg
    23401 acggcgaaaa agatattgcg tatgaactcg gcctgcaaaa gctgccgcct gtcagtgttg
    23461 agcaggcgct gaacgtgtca ttcagcagtc aggtccggga tttatacgac catcagagct
    23521 ggattaagga ccagataatc ccgtccacca cgtctgatga tgaaaccatt atcaaaacag
    23581 caaagtatga aggggttatc cgcaagcagg ccacctttgc cagcgggcgg gtgaccttca
    23641 ccagtcagtc cccccttccg gcagaaaccc ggatgcagtc cgacacgaac caggtgtatc
    23701 aggtactcac gtccggcgag gtgcaggacg gcgaggtcac cgtcatcgtg caggctgaag
    23761 aagccggtgt ggcgggcaat cttgctgccg gtgccgtact caccctgctg tccccgctgc
    23821 ccggaacggc cagcacaggc gcggtggctg aaggtggcat caccggtggc gcagacactg
    23881 aatccatcgc agcgctgctg gaccgtctgc tgtatgtgcg ccgcaatcca ccgacaggcg
    23941 gcgcgttgca tgactacgtg atatgggcgc gtgaagtgcc gggcgtcagc cgcgcgtggg
    24001 cgtgggatgc atggcacggc cccggcacgg tcgggctggc atggctttat gatgaccggg
    24061 aaaacatcgt gccaacccgc gaagacctga agacaatgga gcagtatctg ttctgccaca
    24121 aacacccggc cacgggcgtg atggtgggca aaccgggcgg cattgaggtc tggccggtac
    24181 aggtcaggct gaagaaagtc agcatatcca tccgtctgac accggacagc ctggcgaaca
    24241 ggagcgccgt ccgggcaaac ctgaccgcgt tacagaagac actggcaccc ggtcagacgc
    24301 tgccggtgtc ctcgctgcgc acggccatcg gtatgacgtc cggcatcacg gattacttcc
    24361 ttaacatcgg ggaggacacc accagtgatg tggatgagct tatcaccatc ggggaggtga
    24421 catggctcac agcgtgacgg aatggctggc cgcactgcaa caggtcatgc cacggggtaa
    24481 ggcatggccg cgtgataacg acgcggattt aaaccgcttt ttaagggcgc ttgcagaacg
    24541 tttaaaccgc actgaatacg acgcctcgcg tctgcatgtg gagatgcgcc cggaaaccac
    24601 gctccggctg ctgccggagt gggagcagta tctggcgctg ccggaatgcg gaattgcccc
    24661 caccaccagg gaagcccgcc gccgtgccgt ggtggagaaa taccgtcgca ggggcgggct
    24721 ggcaacctgg cagactgaag ccgcagcagc ggcgctgggc ttcaccatta aggtgacagc
    24781 cgttctgccg caccactgtc tgcgcgactg catgtatccg ctgcatccgg cgcggtatcg
    24841 ctggatgctg aaagtgaacg tcccgggtaa agatgccggg cggtttacct gtattgatgg
    24901 cgtcatgaca ccattaatca gcgaacggac ccgcgagctg gaatgcctgc tgaagcatta
    24961 ccggctggcg ggcacggaat atgaatatta ttacaccgga gaataattta tgttttatgt
    25021 ggataacccg acgggtgtgc cggtaatgcc acctgtcgcg gcagaattaa gcaaaacaac
    25081 gctttatttc accgaaggcg ggaacggtat tccgcccacc tatccggggc cggactggtt
    25141 taatattatt cagtctgaat tactggaaat actccggcag gcaaatatca aaccggataa
    25201 aaacacaacc gaccagatta tgacggcgct gaaaaagctg tttattacga acagtggttc
    25261 agccggtgct attgccggat taaccggtca gaataataca ttcccgtatt ttaccggcaa
    25321 agacacaatg gcattaacac cgctcagtgc ttttgtgcgc ggtattctcg gcaaagagag
    25381 tgcaacagat tttgcagatg cactgaaagt cataaaacaa tcaggcggca cgatgacagg
    25441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
    25501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggcgagaa
    25561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
    25621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
    25681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gatt
//
LOCUS       DAZOYS010000040        25637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOYS010000040 DAZOYS010000000
VERSION     DAZOYS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.040"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHUN9_001454"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHUN9_001454"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0694893.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHUN9_001455"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHUN9_001455"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0694894.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHUN9_001456"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHUN9_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0694895.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHUN9_001457"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHUN9_001457"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0694896.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHUN9_001458"
     CDS             complement(5488..5685)
                     /locus_tag="ACHUN9_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0694897.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHUN9_001459"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHUN9_001459"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0694898.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHUN9_001460"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHUN9_001460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0694899.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHUN9_001461"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHUN9_001461"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0694900.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHUN9_001462"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHUN9_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0694901.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHUN9_001463"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHUN9_001463"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0694902.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHUN9_001464"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHUN9_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0694903.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHUN9_001465"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHUN9_001465"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0694904.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHUN9_001466"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHUN9_001466"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0694905.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHUN9_001467"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHUN9_001467"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0694906.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHUN9_001468"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHUN9_001468"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0694907.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHUN9_001469"
     CDS             complement(17127..17180)
                     /locus_tag="ACHUN9_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0694908.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="ACHUN9_001470"
     tRNA            complement(17414..17489)
                     /locus_tag="ACHUN9_001470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="ACHUN9_001471"
     tRNA            complement(17525..17600)
                     /locus_tag="ACHUN9_001471"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHUN9_001472"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHUN9_001472"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHUN9_001473"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHUN9_001473"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0694909.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHUN9_001474"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHUN9_001474"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0694910.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHUN9_001475"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHUN9_001475"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0694911.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHUN9_001476"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHUN9_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0694912.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEVLQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHUN9_001477"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHUN9_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0694913.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7192 c         6501 g         6016 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggtgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAZOYS010000041        25441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOYS010000041 DAZOYS010000000
VERSION     DAZOYS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.041"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="ACHUN9_001478"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="ACHUN9_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0694914.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="ACHUN9_001479"
                     /pseudo
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="ACHUN9_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="ACHUN9_001480"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="ACHUN9_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0694915.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="ACHUN9_001481"
     CDS             2861..3529
                     /locus_tag="ACHUN9_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0694916.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="ACHUN9_001482"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="ACHUN9_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0694917.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="ACHUN9_001483"
     CDS             4072..5904
                     /locus_tag="ACHUN9_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0694918.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5894..6244
                     /locus_tag="ACHUN9_001484"
     CDS             5894..6244
                     /locus_tag="ACHUN9_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0694919.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHUN9_001485"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHUN9_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0694920.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="ACHUN9_001486"
     CDS             6563..6985
                     /locus_tag="ACHUN9_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0694921.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="ACHUN9_001487"
     CDS             6988..7620
                     /locus_tag="ACHUN9_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0694922.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="ACHUN9_001488"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="ACHUN9_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0694923.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /gene="pduN"
                     /locus_tag="ACHUN9_001489"
     CDS             8112..8387
                     /gene="pduN"
                     /locus_tag="ACHUN9_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0694924.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="ACHUN9_001490"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="ACHUN9_001490"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0694925.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="ACHUN9_001491"
                     /pseudo
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="ACHUN9_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10799..11911
                     /locus_tag="ACHUN9_001492"
     CDS             10799..11911
                     /locus_tag="ACHUN9_001492"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0694926.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11908..13251
                     /locus_tag="ACHUN9_001493"
     CDS             11908..13251
                     /locus_tag="ACHUN9_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0694927.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13254..13808
                     /gene="pduT"
                     /locus_tag="ACHUN9_001494"
     CDS             13254..13808
                     /gene="pduT"
                     /locus_tag="ACHUN9_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0694928.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13808..14158
                     /gene="pduU"
                     /locus_tag="ACHUN9_001495"
     CDS             13808..14158
                     /gene="pduU"
                     /locus_tag="ACHUN9_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0694929.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14163..14606
                     /locus_tag="ACHUN9_001496"
     CDS             14163..14606
                     /locus_tag="ACHUN9_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0694930.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14707..15036)
                     /locus_tag="ACHUN9_001497"
     CDS             complement(14707..15036)
                     /locus_tag="ACHUN9_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0694931.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15208..16266)
                     /locus_tag="ACHUN9_001498"
     CDS             complement(15208..16266)
                     /locus_tag="ACHUN9_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0694932.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHUN9_001499"
     CDS             complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHUN9_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0694933.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHUN9_001500"
     CDS             complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHUN9_001500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0694934.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHUN9_001501"
     CDS             18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHUN9_001501"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0694935.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHUN9_001502"
     CDS             complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHUN9_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0694936.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHUN9_001503"
     CDS             complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHUN9_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0694937.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHUN9_001504"
     CDS             complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHUN9_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0694938.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHUN9_001505"
     CDS             complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHUN9_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0694939.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23004..23933)
                     /locus_tag="ACHUN9_001506"
     CDS             complement(23004..23933)
                     /locus_tag="ACHUN9_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0694940.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23979..24803)
                     /locus_tag="ACHUN9_001507"
     CDS             complement(23979..24803)
                     /locus_tag="ACHUN9_001507"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0694941.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHUN9_001508"
     CDS             complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHUN9_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0694942.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHUN9_001509"
     CDS             complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHUN9_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0694943.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
BASE COUNT         6596 a         6642 c         6254 g         5949 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc ataacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgatag acggctatgg cagaaaaaag
      481 ctgcacgcta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aaacgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgc gatactaaag gcggtgccgg
      721 acacggctca ctgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaagg
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cacccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccgatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccttggcg caaggcggtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtccc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtaattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggaggaaa ccaccggaat caaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggttcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaag
     5461 tacccgttag caaaagtaga aagcctgttc cacttacgcc atgaagacgg tagcgttcag
     5521 ttcttcccta ctcccctatc cccccatgta tttgcccgcg tctgtgtagt gaaacccgac
     5581 gaactggtgc ctattcccgg tgatttgacg ctggaaaaag tacgcgctgt tcgtcgcagt
     5641 gcgaaagagc gtgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaacgcg gtagctactg ggctactcat cgcctggcac
     5881 aaggagtcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaggtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg ctgctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga ccgggaagaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gacgtatcaa caagataacc atacccggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaaggaatt cccttccggg aattccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgcggtgat gttggcgcag
     6421 taaaggccgc cgttgatgcg gggagcgctg cggcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttgct aaggagcaca gcgtgaagca atcactggga ttacttgaag ccagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcat cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaat tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcatctatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat cgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcagccgat ctaccttcat cataatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccgggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc cgcgttggaa aacgcgatcg atagtgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cggctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc gacgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggaa aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgtcgatgaa gcggtctgcg cagcacacta ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcactcgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacttgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccgatg atagttgggt tacttgtgag caactaatgc caatgctgcc cattgtgaag
    10501 gtcaaggatt ttgacagcgc tctaaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactctca gaacgtctca cgactcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatat ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgact ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggacca ggaagtctca aggtacttag
    10861 ccgtttctct catagacaca tctggattat ttgcgatgcc tttctggcac gctcaccact
    10921 tattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgcc ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgta gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcaattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtatcggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcacagccaa
    11641 acgctatgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaat acaacctgta cgctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggcg ctggcggatg caacgttgcg taccaacccg cgtgcagctg atgcctccgc
    11881 gattgcagaa ctgctcgagg agttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagtaca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgctgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 cccatgttga aagtagatca acagttaatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagaaaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa gccaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcggt taactaccaa
    12781 cagctcgcca cgccgcaact gttactgaca gcgctcacct gttcagagtg caacgtctgc
    12841 gccagtgtcg cctgtccggt aggaatttca ccaatgcgca taaaccgcct gcttaagcag
    12901 gaattacgcg cacaaaatct ccgctacgaa ggtgccttaa atcctgcaga tcccatggca
    12961 aattaccgac tgattcccgt aaaacggctg gtaaccagac ttggcttaac cccgtggtat
    13021 caggatgcgc cattgtctga acaggtgcct cagcccgaaa aagtgacgtt attgttacgc
    13081 cagcacatcg gtgccagcgc aattgcgtgt gtacaaaaag gcgatcgcgt cgttcatggg
    13141 caatgtgtag gtcaaattcc acacggcaca ttaggtgccc ccattcatgc cagtattgat
    13201 ggcatggtca gcgatgtgac agaaaacgcg attacgcttg tgagaggtta accatgtcga
    13261 aagcggtagg tattttagaa cttagcagta tcgcgaaagg gatggaaacc ggcgacgcca
    13321 tgctgaaaag cgccaacgtt gagttactgg tcagcaaaac gctatgcccc ggaaaatttc
    13381 tgctgatgct cggtggcgat gtgggcgcgg tgcaacaggc catcaacagc ggagcgtccc
    13441 ttgctggtga aatgctggtc gacagtcttg tcctgccgaa tattcatccc agcctccttt
    13501 ctgcaatcag cggacttaac catgttgaac agcaccgggc tgtcggcgta gtcgaaacct
    13561 ggagtgtcgc cgcctgcatt gacgctgccg attgtgctgt caaagctgca aatgtcattc
    13621 ttgttcgcgt acatatggct tttggcattg gcggtaaatg ctacatggtg gttgctggcg
    13681 atatttcaga tgttaataac gcggtctctg ccgccagcgt gcgcgcgggg gaaaaaggtt
    13741 tgttggtcta tcggtcggtg attgcccgtc cacatgaagc catgtggcgt cagatggtgg
    13801 agggataatg acaactcagc acacaactga acggatgatt caggaatatg tcccaggtaa
    13861 gcaggtcacg ctggcacatg tcatcgccaa tccagggaag gatctgttta agaaattggg
    13921 tttaccagac accatttccg ctattggtat tctgacaatt acccccagcg aagcatctat
    13981 catcgcatgc gatatcgcca caaaatcagg cgctgtagag atcggttttc ttgatcgttt
    14041 taccggtgcc gtagtgctga ctggtgatgt ttctgcgatc gaatatgcac ttagccaggt
    14101 tacccgtacg ctgggtgaaa tgatgcgctt taccgcctgc cccattacgc ggacctgacg
    14161 ttatgcaacg tatgatgctc atcggcccca gccagtgcgg aaaaacgtca ctcacgcaat
    14221 gtctgcgtgg cgaaaatatt cattatcaga aaacgcaggc aatcgtctgg tccccgacca
    14281 ccattgatac accaggcgaa tacctcgaaa accgcagcct ctacagtgca ctgttggtca
    14341 gtgcctgcga agcggacatt atcgcgctgg tgcttaatgc ggacgcaccg tggtcacctt
    14401 tttcgccagg ctttacctcg tcaatgaatc gcccggtcat tggagtagta accaaagcag
    14461 atttagccaa catggaacaa atatctttgg tgaaatgttg gttacgggaa gctggagcgc
    14521 acaacgtgtt agtaaccagt gcggttaata accatggagt tgcagaactt ttagccttgc
    14581 tgcatacaga agaaggctgt cgctaatttc gacaggttat cagatgggaa tgatgacggt
    14641 gatacacaga aaagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
    14701 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
    14761 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
    14821 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tcccaggctt
    14881 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
    14941 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
    15001 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
    15061 gtcagattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
    15121 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
    15181 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
    15241 tagcaactca agctgatgcg ccgtttccat gttcagccac acataaccat agattggtgt
    15301 ttccactacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
    15361 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
    15421 aaagctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
    15481 taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact ccagcataca
    15541 aaccagattg cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
    15601 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcagga gtttttgtag
    15661 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg
    15721 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
    15781 ccagataccg gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca
    15841 taacgccgta tcaatttcac ctgtcgggga accaacaaca attgccagcg tcaccccaat
    15901 caataatcct tgatacggtt tcttgcccag cgccagccaa ccgcaaagaa acatcgccgc
    15961 cgcgcaccag actaacatca gcggtaacga aattaactcc agttgcagag cgataagacc
    16021 taaaatcgaa cccaacaccg taccgccaat acgttcgaag gcacgaggga caacgttacc
    16081 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
    16141 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
    16201 atgcactatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
    16261 acgcacgcag aaactccgta atgaagatta aaagcgctca taactcatct gtcggccaga
    16321 aggcgcgcct ctgaattatc cgacggacaa accgggaaca gtgtaatttc ccggcaggag
    16381 cgaaaaaatt gaaaggcgca tcttactctt ttcgctttca aaaaaagagt ggtcatcgcg
    16441 ttaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
    16501 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
    16561 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
    16621 aaatcatcac cgactacacg agccaccgcc accgcatact gaccacctga aatttctgtc
    16681 agaatgacgc cctcactgtt ttcgggaagc gtaaagttat tcggtaccgt cacaacggtg
    16741 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac
    16801 tccttcggca caatattttt gttatctacc cacatcatca actgctcaaa gcctttcttt
    16861 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt ctcttcctgc
    16921 gtaatctcgt agttcatgac gcctccatta acactgtttt tatatacagc atagttgcaa
    16981 tattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
    17041 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcggg aaaacatgcc
    17101 gccttccccg acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc
    17161 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gctctttacc
    17221 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagca ccatccagaa
    17281 ctcttgttcc gttcctaggt cgatattttc tttatcgcca taccagatgc gttccgttcc
    17341 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
    17401 taattttctt gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat
    17461 gagacgacgc tgcccatcta cagccgaagc aatgagatta aacccggcac cagaagtatg
    17521 acccgttttc aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg
    17581 ctgggtgata ccgttccagg tgagactttt ctcactgtac atatgataaa actcgggctc
    17641 gccgtggatg atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg
    17701 tgcatccaga ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
    17761 gttgttcatc atttcaacaa actgccgttg cccaccggca atatagtcag ccagagcaac
    17821 acaagcgtca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
    17881 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca
    17941 cgcatcgcgc cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg
    18001 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
    18061 attacccgcg gtgaggatct gaccggtggt gtaatccatc aatacccagg acccggcatg
    18121 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
    18181 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
    18241 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
    18301 aatgtgacta ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
    18361 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg
    18421 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
    18481 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
    18541 tagcgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg ctgatgacta
    18601 tttaccccag ccaggagccg tattaattac cggtattacc ccgcaggaag cacgggcgaa
    18661 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac
    18721 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
    18781 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
    18841 gctggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
    18901 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gaattgaaca
    18961 tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg cgaaactggt
    19021 aaaaacgcgt cagccacgtc tgtttgatta tctctttacc catcgtaata aacacaaact
    19081 gatggcgttg attgatgttc cgcagatgaa acccctggtt cacgtttccg gaatgtttgg
    19141 ggcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatccag aaaatcgcaa
    19201 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga
    19261 cacattgcgc gagcgtttat atactgcaaa agccgatctt ggcgataacg ccgccgttcc
    19321 ggttaagctg gtgcatatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc
    19381 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc tgaaaattct
    19441 gcgtgaaaat ccgcaagtgc gcgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
    19501 tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg acgcagatcg
    19561 tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
    19621 ttttgtcgat aaacggattg aaaagctgtt gttcaattat cgggcacgca acttcccggg
    19681 gacgctggat tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc
    19741 cgagttttta caaggttatg cagatgaatt gcagatgctg gcacaacaat atgccgataa
    19801 caaagagaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctaatt
    19861 tgaagcctcc gccgctggta cggcggaggt caatgttgag tcaggctttt tgaacggtga
    19921 tactccacgc tgcatcgcca atctgctgat aatcggtgat ggcatgccct tcttctgccg
    19981 cccactgtgg gattgcttca gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc
    20041 cgctggccat ttctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg
    20101 tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt
    20161 gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg
    20221 agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt
    20281 ccattaccga taaagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct
    20341 aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct
    20401 ttggctgcaa taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg
    20461 tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct
    20521 aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg
    20581 atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatccca
    20641 gttcggcgcg gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc
    20701 acccagagcg ttatcaccag caaaacggca accaacggcc aaacagacaa attaaaagtc
    20761 tcagcaatag agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca
    20821 tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg
    20881 atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt
    20941 cccagcccga agatataccc acctataaca gtaccaagcc acgggaacgc ctcggcttcg
    21001 taagtcagta ggcccgcctg aattaacgca aaaaccccca cgctttgtac cgaaattgca
    21061 atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacct
    21121 gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg
    21181 cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc
    21241 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
    21301 tgaggatttc gataaaaaaa aataaaaaaa ccggagccaa atattcattc gactccggtt
    21361 gattattaga aaagatgatt atgcaacgtc ttcgtactgc ggaactgggt tacggaagct
    21421 cttggtgacg caagccaggt aaatcagacc gatagcagcc caaatcagac ccagaaccat
    21481 tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag
    21541 gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg
    21601 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc
    21661 tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
    21721 gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac
    21781 gccgtcacga cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga
    21841 cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac
    21901 gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
    21961 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag
    22021 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat
    22081 gccgtcaaag ccggtaaagg aaaagcacag gattgtcgcc ccggtaatca tcggaataac
    22141 atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc
    22201 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
    22261 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
    22321 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata
    22381 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
    22441 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat atgcagagcc
    22501 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
    22561 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
    22621 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
    22681 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
    22741 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
    22801 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
    22861 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
    22921 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
    22981 aagcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
    23041 acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagccct
    23101 gcggtctgtt cgttccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
    23161 ttctcgatta acgcgccact atcgagaaaa cgttgcacca gataacgggg taaatagccg
    23221 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct
    23281 tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc
    23341 acaatcgccc gatatcgctt aatgatacga cggtttaacg gctcttcttc ctgggctaac
    23401 ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac
    23461 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
    23521 agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga
    23581 tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
    23641 ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
    23701 cgcacggtat gcagaacttc ccgccccttc tctaataaca tttttccggt acgggtgaat
    23761 ttagcgcggt gaccgctacg atcaagtaat tgaatattaa ggtcgctttc cagcttgtga
    23821 acggtgtaac tcagagcgga cggcgtcttg tagagtttag ccgacgccgc cgcaaaactg
    23881 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagttgttt catacacgcc
    23941 cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc
    24001 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
    24061 ttacctttgc cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg
    24121 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
    24181 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
    24241 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
    24301 tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt accgggtaaa
    24361 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggtcac cggattacgc
    24421 ggggtggttt ctttcaccgt gccttgcgcg tcgccataga cagacgttga gctggtaaaa
    24481 ataatgcgag gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
    24541 tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag cgcatcggca
    24601 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
    24661 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
    24721 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
    24781 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
    24841 cgctagcgta tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg
    24901 acatgctgca atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt
    24961 aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc
    25021 ttcaaatggc gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt
    25081 atctgtttcc tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac
    25141 tcaataatgt ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
    25201 ctccgggcgt tagcggggga gcgcagtaga taagccgtct cttccagcga gttgtattct
    25261 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga
    25321 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
    25381 gtacgcatgt caatctcctc ttttgtacag tttattgtac aatgatgggc gttaattaac
    25441 t
//
LOCUS       DAZOYS010000042        25381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOYS010000042 DAZOYS010000000
VERSION     DAZOYS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.042"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHUN9_001510"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHUN9_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUN9_001511"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUN9_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0694944.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHUN9_001512"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHUN9_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0694945.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUN9_001513"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUN9_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0694946.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUN9_001514"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUN9_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0694947.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUN9_001515"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUN9_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0694948.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUN9_001516"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUN9_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0694949.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUN9_001517"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUN9_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0694950.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUN9_001518"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUN9_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0694951.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUN9_001519"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUN9_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0694952.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUN9_001520"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUN9_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0694953.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUN9_001521"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUN9_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0694954.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUN9_001522"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUN9_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0694955.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUN9_001523"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUN9_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0694956.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUN9_001524"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUN9_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0694957.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUN9_001525"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUN9_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0694958.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUN9_001526"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUN9_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0694959.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUN9_001527"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUN9_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0694960.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUN9_001528"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUN9_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0694961.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUN9_001529"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUN9_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0694962.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUN9_001530"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUN9_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0694963.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUN9_001531"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUN9_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0694964.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHUN9_001532"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHUN9_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0694965.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUN9_001533"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUN9_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0694966.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUN9_001534"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUN9_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0694967.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUN9_001535"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUN9_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0694968.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUN9_001536"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUN9_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0694969.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUN9_001537"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUN9_001537"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0694970.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUN9_001538"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUN9_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0694971.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHUN9_001539"
     CDS             14267..14635
                     /locus_tag="ACHUN9_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0694972.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUN9_001540"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUN9_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0694973.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUN9_001541"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUN9_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0694974.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUN9_001542"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUN9_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0694975.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUN9_001543"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUN9_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0694976.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUN9_001544"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUN9_001544"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0694977.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUN9_001545"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUN9_001545"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0694978.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUN9_001546"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUN9_001546"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0694979.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUN9_001547"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUN9_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0694980.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHUN9_001548"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHUN9_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0694981.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHUN9_001549"
     CDS             21814..22356
                     /locus_tag="ACHUN9_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0694982.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUN9_001550"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUN9_001550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0694983.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUN9_001551"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUN9_001551"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0694984.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHUN9_001552"
     CDS             23753..24010
                     /locus_tag="ACHUN9_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0694985.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHUN9_001553"
     CDS             complement(23986..24540)
                     /locus_tag="ACHUN9_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0694986.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25381
                     /locus_tag="ACHUN9_001554"
     rRNA            25013..>25381
                     /locus_tag="ACHUN9_001554"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6468 a         6017 c         6649 g         6247 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct caccaaggcg acgatcccta gctggtctga gaggatgacc
    25321 agccacactg gaactgagac acggtccaga ctcctacggg aggcagcagt ggggaatatt
    25381 g
//
LOCUS       DAZOYS010000043        25106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOYS010000043 DAZOYS010000000
VERSION     DAZOYS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.043"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHUN9_001555"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHUN9_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0694987.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUN9_001556"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUN9_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0694988.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHUN9_001557"
     CDS             1145..1309
                     /locus_tag="ACHUN9_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0694989.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUN9_001558"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUN9_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUN9_001559"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUN9_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0694990.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUN9_001560"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUN9_001560"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0694991.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUN9_001561"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUN9_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUN9_001562"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUN9_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0694992.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUN9_001563"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUN9_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0694993.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHUN9_001564"
     CDS             14459..15955
                     /locus_tag="ACHUN9_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0694994.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUN9_001565"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUN9_001565"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0694995.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUN9_001566"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUN9_001566"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0694996.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHUN9_001567"
     CDS             18049..20109
                     /locus_tag="ACHUN9_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0694997.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHUN9_001568"
     CDS             complement(20291..21613)
                     /locus_tag="ACHUN9_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0694998.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASIAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHUN9_001569"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHUN9_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0694999.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHUN9_001570"
     CDS             complement(23086..23985)
                     /locus_tag="ACHUN9_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0695000.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHUN9_001571"
     CDS             24505..>25106
                     /locus_tag="ACHUN9_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0695001.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6364 c         6992 g         5856 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaatggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tagtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacgg ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt actgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZOYS010000044        24465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOYS010000044 DAZOYS010000000
VERSION     DAZOYS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.044"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHUN9_001572"
     CDS             <1..228
                     /locus_tag="ACHUN9_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0695002.1"
                     /translation="LYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAE
                     LLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            384..1919
                     /gene="gadC"
                     /locus_tag="ACHUN9_001573"
     CDS             384..1919
                     /gene="gadC"
                     /locus_tag="ACHUN9_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0695003.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2050..3369
                     /locus_tag="ACHUN9_001574"
     CDS             2050..3369
                     /locus_tag="ACHUN9_001574"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0695004.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3741..5123
                     /gene="dosC"
                     /locus_tag="ACHUN9_001575"
     CDS             3741..5123
                     /gene="dosC"
                     /locus_tag="ACHUN9_001575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0695005.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5148..7547
                     /gene="dosP"
                     /locus_tag="ACHUN9_001576"
                     /gene_synonym="pdeO"
     CDS             5148..7547
                     /gene="dosP"
                     /locus_tag="ACHUN9_001576"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0695006.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHUN9_001577"
     CDS             7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHUN9_001577"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0695007.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8401..9951
                     /locus_tag="ACHUN9_001578"
     CDS             8401..9951
                     /locus_tag="ACHUN9_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0695008.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9953..10975
                     /locus_tag="ACHUN9_001579"
     CDS             9953..10975
                     /locus_tag="ACHUN9_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0695009.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHUN9_001580"
     CDS             10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHUN9_001580"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0695010.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11865..12851
                     /locus_tag="ACHUN9_001581"
     CDS             11865..12851
                     /locus_tag="ACHUN9_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0695011.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12880..13776
                     /locus_tag="ACHUN9_001582"
     CDS             12880..13776
                     /locus_tag="ACHUN9_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0695012.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(13832..14263)
                     /gene="osmC"
                     /locus_tag="ACHUN9_001583"
     CDS             complement(13832..14263)
                     /gene="osmC"
                     /locus_tag="ACHUN9_001583"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0695013.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14608..14823
                     /gene="bdm"
                     /locus_tag="ACHUN9_001584"
     CDS             14608..14823
                     /gene="bdm"
                     /locus_tag="ACHUN9_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0695014.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14925..15062
                     /gene="sra"
                     /locus_tag="ACHUN9_001585"
     CDS             14925..15062
                     /gene="sra"
                     /locus_tag="ACHUN9_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0695015.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15219..16916
                     /gene="maeA"
                     /locus_tag="ACHUN9_001586"
     CDS             15219..16916
                     /gene="maeA"
                     /locus_tag="ACHUN9_001586"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0695016.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17050..18052
                     /gene="adhP"
                     /locus_tag="ACHUN9_001587"
                     /pseudo
     CDS             17050..18052
                     /gene="adhP"
                     /locus_tag="ACHUN9_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18198..18482
                     /locus_tag="ACHUN9_001588"
     CDS             18198..18482
                     /locus_tag="ACHUN9_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0695017.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18710..19363)
                     /gene="fdnI"
                     /locus_tag="ACHUN9_001589"
     CDS             complement(18710..19363)
                     /gene="fdnI"
                     /locus_tag="ACHUN9_001589"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0695018.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19356..20240)
                     /gene="fdnH"
                     /locus_tag="ACHUN9_001590"
     CDS             complement(19356..20240)
                     /gene="fdnH"
                     /locus_tag="ACHUN9_001590"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0695019.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20253..23300)
                     /gene="fdnG"
                     /locus_tag="ACHUN9_001591"
     CDS             complement(20253..23300)
                     /gene="fdnG"
                     /locus_tag="ACHUN9_001591"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22713..22715),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0695020.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23532..24413
                     /gene="yddG"
                     /locus_tag="ACHUN9_001592"
     CDS             23532..24413
                     /gene="yddG"
                     /locus_tag="ACHUN9_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0695021.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5996 a         6209 c         6190 g         6070 t
ORIGIN      
        1 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
       61 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg
      121 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac
      181 ccgaaactgc agggtattgc ccagcagaac agctttaaac atacctgata acgtttaacg
      241 gtaacggtgt cccgaaacga acccgtttcg ggataatttc caaagtctgt tcactggcat
      301 tagcaacgga aaatattgtt ctgaatacgc ttcagaacaa aacaggtgcg gttccgacag
      361 gaataccgtt ttagggggat aatatggcta catcagtaca gacaggtaaa gctaagcagc
      421 tcacattact cggctttttt gccataacgg catcgatggt aatggctgtt tatgaatacc
      481 ctaccttcgc aacatcgggc ttttcattag tcttcttcct gctattaggc gggattttat
      541 ggtttattcc cgtgggactt tgtgctgcgg aaatggccac cgtcgacggc tgggaagaag
      601 gtggtgtctt cgcctgggta tcaaatactc tggggccgag atggggattt gcagcgatct
      661 catttggcta tctgcaaatc gccattggtt ttattccgat gctctatttc gtgttagggg
      721 cactctccta catcctgaaa tggccagcgc tgaatgaaga ccccattacc aaaactattg
      781 cagcactcat cattctttgg gcgctggcat taacgcagtt tggtggcacg aaatacacgg
      841 cacgaattgc taaagttggc ttcttcgccg gtatcctgtt acctgcattt attttgatcg
      901 cattagcggc tatttatctg cactccggtg cccccgttgc tatcgaaatg gattcaaaga
      961 ccttcttccc tgacttctct aaagtgggca ccctggttgt atttgttgcc ttcattttga
     1021 gttatatggg cgtagaagca tccgcaaccc acgtcaatga aatgagcaac ccagggcgcg
     1081 actatccgtt ggctatgtta ctgctgatgg tagcggcaat ctgcttaagc tctgttggtg
     1141 gtttgtctat tgcgatggtc attccgggta atgaaatcaa cctctccgca ggggtaatgc
     1201 aaacctttac cgttctgatg tcccatgtgg caccggaaat tgagtggacg gttcgcgtga
     1261 tctccgcact gctgttgctg ggtgttctgg cggaaatcgc ctcctggatt gttggtcctt
     1321 ctcgcgggat gtatgtcaca gcgcagaaaa acctgctgcc agcggcattc gctaaaatga
     1381 acaaaaatgg cgtaccggta acgctggtca tttcacagct ggtgattact tctatcgcgt
     1441 tgatcatcct caccaatacc ggtggcggta acaacatgtc cttcctgatc gcactggcgc
     1501 tgacggtggt gatttatctg tgtgcttatt tcatgctgtt tattggctac attgtgttgg
     1561 ttcttaaaca tcctgactta aaacgcacat ttaatatccc tggtggtaaa ggggtgaaac
     1621 tggtcgtggc tattgtcggt ctgctgactt caattatggc gtttattgtt tccttcctgc
     1681 cgccggataa catccagggt gactctaccg atatgtatgt tgaattactg gtcgttagtt
     1741 tcctggtggt acttgccctg ccctttattc tctatgctgt tcatgatcgt aaaggcaaag
     1801 cgaataccgg cgtcactctg gagccaatca acagtcagaa cgcaccaaaa ggtcacttct
     1861 tcctgcaccc gcgtgcacgt tcaccacact atattgtgat gaatgacaag aaacactaac
     1921 catgtctgaa tggttataag acaagggagc gataattcat cgctcccttt ttcgtgctcg
     1981 ctgtcgtatt gacttaaccg ggataagtac gagaatgagc gcacatctgt ttaccggaaa
     2041 ccagcatata tggatatctg ctcccgaaac gagaaattag cgattagaag accagcgata
     2101 ctggttgcac tggcactttt actgtgtagt tgtaaaagca cgcctccaga gtccatggtg
     2161 acaccaccag caggttcaaa gccaccagcc acgacgcaac aatcgtcaca accgatgcgt
     2221 ggcatctggc tggccacggt gtctcgcctc gactggccac cggtttcctc ggttaacatt
     2281 agtaacccca ccagccgggc ccgtgtacaa caacaggcga tgatcgacaa actggatcat
     2341 ctgcaacgtc tcggtataaa cacggtcttt ttccaggtta agccggacgg taccgccctg
     2401 tggccatcga aaattttgcc atggtccgat cttatgaccg gtaagattgg tgaaaatccg
     2461 ggttacgatc cgctgcaatt tatgctcgac gaagcccaca agcgtgggat gaaagtacac
     2521 gcctggttta acccctatcg cgtatcggtt aatacgaagc ccggtactat cagggaactg
     2581 aatagcactc tgtctcaaca accggcgagc gtctatgtgc aacaccgtga ctggatcaga
     2641 acgtctggcg atcgctttgt cctcgacccg ggcatccctg aggttcagga ctggatcaca
     2701 tcaatagtcg cagaagtggt ttcccgctat ccggtagatg gcgtgcagtt tgacgactat
     2761 ttctatactg agtcaccggg ttcacggcta aatgataacg aaacgtaccg caaatacgga
     2821 ggcgcatttg cgtcaaaagc agactggcgg cgcaacaata ctcagcagtt aattgcaaaa
     2881 gtatcgcaca ccattaaaag cattaagccg ggagtcgaat ttggcgttag cccggcaggc
     2941 gtgtggcgta accgatcaca cgatccgctc ggttccgata cccgaggcgc ggcagcctat
     3001 gacgaatcct acgctgacac ccgtcgatgg gtggaacaag gattgctgga ttacattgct
     3061 ccccaaattt actggccgtt ctcacggagt gccgcgcgtt atgacgtgtt ggcaaaatgg
     3121 tgggcggatg tcgttaaacc gaccaggacc cgcctgtata tcggtatcgc cttctataaa
     3181 gtgggtgaac cttcaaagat agagccagac tggatgatta acggcggcgt accggaactg
     3241 aaaaagcagc tcgatcttaa cgatgcggta ccagaaatta gcggcaccat cttgttccgt
     3301 gaggactatc tgaataaacc gcagactcaa caagcggtca gctatctgca aagtcgttgg
     3361 ggcagttaaa ctcccatgct tactttgtaa caagccggtg ctttacccac cggcttgtta
     3421 caccttgtga aatatcccac tagtaatcat gcttacataa gtcaaattaa ccacgcaatt
     3481 aaccacactt agttaaacag ctataaatcc ctaataaaaa tgtatgaata tgaaaaacca
     3541 tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct gaagaacagt
     3601 agcgtagatt gaaactgtcc taagtcgctt atttcttttc agtatatctt catatttcag
     3661 gagaatatgg tgcagttagt caatctcgca cgttgcgttt tgattccgtc tgatttccca
     3721 gccttataag ggtgtgaatt atggagatgt attttaaaag aatgaaagat gagtggaccg
     3781 gacttgtcga acaggcagat ccactcattc gtgctaaagc cgcggaaatt gccgttgcgc
     3841 atgctcatta tctgagtatc gagttttatc gaattgtccg catcgacccg catgccgaag
     3901 aattcttgag taatgaacaa gttgagcggc agttgaaaag tgcgatggaa cgctggatta
     3961 ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct aatacaaatc cagcataccg
     4021 tcgcggaagt gcatgcccgc ataggaattc cggtagaaat tgtcgagatg gggtttcggg
     4081 tgctgaaaaa aatcctctat ccggtaatct tctcttcgga ttattccgcc gcagaaaaac
     4141 ttcaggtcta tcatttctcg attaacagta ttgatatcgc gatggaagtg atgacccgcg
     4201 cgtttacctt tagtgacagt agtgcctcaa aggaagatga aaattatcgt atcttctcgt
     4261 tactggaaaa cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt tcatgggaaa
     4321 tagatattat ctataaaatc ctgctggatt ctgatttagg cagtagtttg ccattaagcc
     4381 aggctgattt tggcctgtgg tttaaccata aaggtcgaca ttattttagt ggcattgccg
     4441 aagtaggcca tatctcccgt ctcattcagg atttcgacgg tattttcaat caaaccatgc
     4501 gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa atttttatta cagataagaa
     4561 ataccgtatc gcagattatt accttgctgc gtgaattgtt tgaagaagta tcgcgccacg
     4621 aagtcggtat ggatgtacta acgaaattac ttaaccgccg tttcctgccg actatcttca
     4681 aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg attattgacg
     4741 ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat gaaattctgc
     4801 gtaaagtctc tcaggccttt tatgacaacg tccacagtag tgattatgtt tttcgctacg
     4861 ggggcgatga atttatcatt gttttgactg aagcttctga aaacgaaacg ttacgtaccg
     4921 cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa agccgcaaac ggcgaggata
     4981 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     5041 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     5101 aactctggaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     5161 atgcggaaaa tgccgccgat ggcatttttt tccccgccct tgaacaaaat atgatgggcg
     5221 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     5281 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccgcgggatt
     5341 tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga aggtggtaaa gcgcgcgtcg
     5401 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     5461 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     5521 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     5581 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     5641 ttaccgaaat gtttggttac tgcattagcg aagccagcgg tatgcagccc gatacactcc
     5701 tgaacattcc tgaattccct gccgataacc gcattcgttt acaacagttg ctatggaaaa
     5761 ccgcccgcga tcaggacgaa tttctgctgt tgacacgcac cggtgaaaaa atctggatta
     5821 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacct
     5881 tctcggatat caccgaagaa cggcagatcc gccagcttga aggcaatatt ctcgccgcca
     5941 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     6001 ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa cgggatgccg atacactggg
     6061 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     6121 agcgtgatgg tgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     6181 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     6241 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     6301 caaatcgcaa taacctgcac aattacctcg atgaccttgt cgacaaagcc gtctctccgg
     6361 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     6421 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     6481 agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt gagcctggaa aacgacgtca
     6541 gtaacattac ccaaatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     6601 acgataaacc attcccgcta accttgagta ttggcatcag ctacgacgtg ggtaaaaacc
     6661 gcgattacct gctctccact gctcacaatg caatggatta tattcgcaag aatggcggta
     6721 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     6781 gcgcagcgct gaaagaagcg attagcaata accaactgaa actggtttac cagccgcaaa
     6841 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg cacgatcccc
     6901 agcatggtca tgtaccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6961 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     7021 agaatattca tatcccggcg ttatccgtga acttgtcggc gctgcacttt cgcagtaatc
     7081 agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg tattgacggc caccagctga
     7141 cggtagaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     7201 agatcctgcg cgatatgggc gtgggcttat cggtagatga ttttggcacg ggcttttccg
     7261 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgacaaa agttttgtcg
     7321 atcgttgtct gacggaaaaa cgcatccttg ccttacttga agccattacc agcattgggc
     7381 aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     7441 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctacccgccg
     7501 aggaaattcc aggctggatg agcagcgtgt taccgctgaa aatctgacaa attcctctcg
     7561 cccgcactcg cgggttttca tttaacgtga cactgtcact taaatactgt gatttcagct
     7621 acgattccgg gagattcctt ccttaacttc ccctcttgca ccaaaactgc actacgctgc
     7681 acaaatgttg atcaaaagtt aaaaaatata actttaagcc agcattctgg cgcttatccc
     7741 ggcatggcat gagatctgca taagcggaaa gcgcagcaat ttttgtctta atacaggaac
     7801 cgtttatgtc ggataccacc gaactggttg atttagccgt gatcttccct gatctggaga
     7861 tcgaattgaa atacgcctgc gctgataaca tcacaggtaa agctatttac cagcaagcgc
     7921 gttgtctgtt acacaaggat gcgattaccg cgctggcgaa aagtatcagt atcgcccagc
     7981 tgtcaggctt acaactggtg atttacgatg cgtatcgccc acaacaagca caggcgatgt
     8041 tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca
     8101 gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca cgggaacatc ctggatatgg
     8161 gcgcagggtt cgatgaaatg cacgagcgct cccatgccta tcacccttcc gtcccgcccg
     8221 ccgctcagcg caatcgcctg ttgctgaatg cgataatgac tggtggcggt tttgtcggta
     8281 tctccagcga atggtggcac ttcgaattac cccaggcagc gagttaccct ctgctcgctg
     8341 atcaattctc ttgttttata tcccctggca cacagcacgt cagttaatca ggaaactgtc
     8401 atgaagagat cgatatcgtt tcgtcccaca ttgctcgcgc tcgtccttgc cacaactttc
     8461 ccggttgcgc acgccgccgt accgaaagat atgctggtga ttggtaaggc cgccgatcca
     8521 caaaccctcg acccggcggt aacaatagat aataacgact ggacagtgac ctacccgtct
     8581 tatcagcggc tggttcagta caaaacggac ggtgataaag gctcaaccga cgttgaaggc
     8641 gatctggcaa gtagctggaa agcgtctgac gatcaaaaag agtggacgtt caccctgaag
     8701 gacaatgcta aatttgccga tggcacacct gtcactgccg aagcagtaaa actctctttt
     8761 gagcggttac taaaaatcgg ccaggggcca gcagaagcat ttcccaaaga tttaaagatt
     8821 gatactcccg acgaacatac ggtgaagttt acccttagcc aaccattcgc accgttcctc
     8881 tacacgctag cgaatgacgg tgcatccatt atcaatccgg cggtcttaaa ggaacatgca
     8941 gcggatgatg ctcgcggctt cctcgcgcaa aataccgccg gttccggacc atttatgctg
     9001 aaaagctggc aaaaaggtca gcaattagtt ctggtgccaa atccgcatta ccccggcaat
     9061 aaaccgaact ttaagcgagt atcggtaaaa attatcggtg aaagtgcctc ccgtcgcctg
     9121 cagctctccc gtggcgacat tgacattgcc gatgcgctgc cggtggatca actcaacgcc
     9181 ctgaagcagg aaaataaagt caatgtggca gagtatccgt cactgcgcgt tacctatctg
     9241 tatctgaata acagcaaagc gcctcttaat caggcggatc tgcgtcgggc catttcctgg
     9301 tctaccgatt atcagggcat ggttaacggc attctgagtg gtaacggaaa acagatgcgc
     9361 ggcccgattc cggaaggcat gtggggctac gatgcgacgg caatgcaata caaccatgac
     9421 gaaacgaaag ccaaagccga atgggataaa gtgacgagca aacccaccag cctgacgttt
     9481 ctctactccg ataacgatcc gaactgggag cctattgctc tggcgacaca atccagtctc
     9541 aacaagctgg gcatcaatgt gaagctggaa aagctggcga acgccaccat gcgcgacaga
     9601 gtgggtaaag gtgattacga cattgcgatt ggcaactgga gtccggattt tgccgacccg
     9661 tatatgttta tgaattactg gtttgagtca gacaaaaaag gtctgccggg taaccgctcg
     9721 ttctatgaaa acagtgaggt cgataagtta ctgcgcaatg cgcttgcgac caccgaccag
     9781 acgcagcgta cccgggacta ccagcaggca cagaaaatcg tcattgatga cgctgcttat
     9841 gtgtacctgt tccagaaaaa ctaccaactg gcgatgaaca aagaggtgaa aggctttgtg
     9901 ttcaatccca tgctggaaca ggtcttcaat atcaatacca tgagtaaata acgtgacctt
     9961 ctggagtatt ttacgccaac gctgctgggg gctggtgctc gtggtggcgg gcgtctgcgt
    10021 gattacgttt attatctcgc atctgatccc tggcgatccg gcgcggttac tggcgggtga
    10081 ccgcgccagc gatgctatcg tggaaaatat tcgccagcaa ctgggactgg accagccact
    10141 gtatgtacag ttttaccgct acttcagcga cctgtttcat ggtgacctgg gaacatccat
    10201 tcgtaccggg cgtccggtgc tggaagagtt gcgtatattt ttcccggcga cgctggaact
    10261 ggctttttgt gccctgctgc tggcactcct gattggcatc ccgctgggca tactctctgc
    10321 agtctggcga aatcgctggc tggatcatct ggtgcgaata atggccatta ccggaatctc
    10381 cacacctgcg ttctggcttg gactgggcgt cattgtgctg ttttatggtc atctgcaaat
    10441 tcttcccggc ggcggaaggc ttgatgactg gctggatcca ccaacgcacg ttaccggctt
    10501 ttatctgctc gatgcgctgc ttgaaggcaa cggtgaagtc ttcttcaatg cgttgcaaca
    10561 tctcatctta ccggcattaa cgctggcgtt cgttcacctg ggaattgtcg ctcgccagat
    10621 ccgctcagcg atgctggaac aattgagtga agactacatt cgtaccgccc gggccagcgg
    10681 cttgcccggc tggtatatcg ttttatgtta tgcgctaccc aatgcgttga tcccatcgat
    10741 taccgtattg ggtctggcgc tgggcgattt gttgtatggc gcagtgctca ccgaaaccgt
    10801 ttttgcctgg cccggaatgg gtgcatgggt agtaacatca atacaggcgc tcgacttccc
    10861 ggcagtgatg ggctttgccg tcgtggtttc atttgcttat gtgctggtca acctggtggt
    10921 ggatttgctc tatttgtgga ttgatccgcg tatcggacgt ggaggtggtg aatgatgcta
    10981 agcgaggaaa cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg tgcaaaactg
    11041 gtatggatgc tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat tgtattaatg
    11101 ctattgatga tgattttttc accgtggctt gcgacgcatg atcccaacgc cattgattta
    11161 accgcccgcc ttttgccgcc ttctgcggcg cactggtttg gcaccgatga agtgggacgc
    11221 gatctgttca gccgcgtact ggtcggcagt cagcaatcaa ttctcgccgg attagtcgtg
    11281 gttgccattg cgggtatgat tggttcgcta ctcggatgtc tatccggtgt gcttggcgga
    11341 cgcgcagacg ccatcatcat gcgcatcatg gacattatgc tgtcgattcc ttcgttggta
    11401 ctgacaatgg cactggctgc cgctctcggg ccgagtttat ttaacgccat gctggcgatt
    11461 gctattgtgc gaattccctt ttatgtgcgc ctggcgcggg ggcaagcatt agttgtacgc
    11521 cagtatacct atgttcaggc ggcgaaaacc tttggtgcgt cgcgttggca tctgatcagc
    11581 tggcatattt tacgtaactc cctgccgccg ctgatcgtgc aggcatcgct ggatatcggt
    11641 agcgcgattt taatggccgc cacgttggga tttattggcc tgggtgctca acaaccgagt
    11701 gctgaatggg gcgcgatggt agctaatggt cgcaactatg tactcgatca gtggtggtat
    11761 tgcgcatttc cgggggcagc gattttgctt accgccgtcg ggtttaatct ctttggtgat
    11821 ggtattcgcg atctgcttga cccgaaagca ggaggaaagc agtcatgacc caacccgttc
    11881 tggacattca acagctgcat ttgagtttcc ccggttttaa cggttatgtt cacgtgctca
    11941 acaatgtgtc cttgcagatt aaccgcggtg aaattgtcgg tctggtggga gaatccggct
    12001 caggtaaatc agtcaccgca atgctgatta tgcgtctgct accgacgggc agttattgcg
    12061 tacatcgggg gcagatttca ctgctgggag acgatgttct taacgctcgg gagaaacagc
    12121 ttcgtcagtg gcgcggcgca cgagtggcga tgatctttca ggaaccgatg accgccctca
    12181 atccgacacg tcgaataggt cttcagatga tggacgtgat ccgccatcat caaccaataa
    12241 gtcgtcggga agccagagct aaagcgatta ccctgctgga agagatgcaa atcccggatg
    12301 ccgtggaagt tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc cagcgggtaa
    12361 tgattgcgct ggcattctcc tgcgagccgc aattgattat tgccgacgaa ccgactacgg
    12421 cgctggacgt cacggtacag ttgcaggtac tgcgtttgct taaacataaa gctcgcgcca
    12481 gtggaactgc ggtactgttc atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata
    12541 gcgtttacgt gatgtatgcc ggaagcgtaa ttgaaagcgg cgtgacggca gacgttatcc
    12601 atcatccccg gcatccgtat accattggtt tgctgcaatg cgcaccagaa catggagtac
    12661 cacgccagcc attacccgcc attccaggga cggtaccaaa cctcacccat ttgcctgacg
    12721 gctgcgcttt tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc
    12781 tgacagcgtg tggtgacaac aaccagcgct gcgcctgttg gtatccgcag caggaggtca
    12841 taagtatctg acacgttatt aacgttacgc gacgtccata tcaatttccc ggcccgtaaa
    12901 agctggcttg gtaaaactac ggaacatgtt catgcgatta atggtattga tttacagatc
    12961 cgccgtggtg aaaccttagg gatcgtcggt gagtcaggct gcggcaaaag caccctcgca
    13021 cagcttttaa tgggtatgct gcaaccgagc cacgggcaat acatccgttc aggatcacaa
    13081 cgcattatgc agatggtgtt tcaggacccg ctctcttcgc ttaatccgcg cttatcggtg
    13141 tggcgcatca tcacagaacc gctctggata gctaagcgta gtagtgaaca acagcggcga
    13201 gcgttggcaa aggagttggc tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg
    13261 cctcatgcgt tctccggcgg gcagcggcaa cgcatcgcca ttgccattgc cagagcactc
    13321 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
    13381 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
    13441 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
    13501 ctcgggcaga ttgtagaact gggcgacgcg cagcaggtgc tgacggcacc tgcacatcca
    13561 tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
    13621 gcattatgta aaacggatct gccgggaaac cgcacgttgc cacaaggctg ttttttctac
    13681 gaacgttgcc ctctggcaac ccacggatgt gaagtccggc aatcattagc aataagggag
    13741 gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
    13801 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tccagcgtaa
    13861 tttccgcttt cagtacctga gaaaccgggc atcctgcttt tgctttctgg atgatgccgt
    13921 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
    13981 tcgcaaaatc ggcatccact ttatccagcg acacatcggc ggtggtatca atcgatgttg
    14041 gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
    14101 ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc
    14161 catacggctg ttggttcagc acgccactct gggtggatac tgttcccttc ccgcgtttga
    14221 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
    14281 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
    14341 ataaaatacg gcgaaaatat tacttctgca cttaaattaa attcccacaa tagataaccg
    14401 ggaatatata tctcaagaga tattgcatca atatcaatca actcaccaca ataataatac
    14461 atatcaatat catggagtta aaatataaaa atcatcaacc aggactaatc ttaacaacga
    14521 agcggcaaat atttgccatg ctgaatgtgc tttataaatc tgcgatccgt agcagacacc
    14581 ataaatacac agacacggag aatcactatg tttacttatt atcaggcaga aaattcaaca
    14641 gcagaacccg ctctggttaa tgccattgag caaggccttc gggcagagca aggtgttgtc
    14701 actgaagatg acattctcat ggagctgacc aaatgggtgg aagcttctga taacgacatc
    14761 ctcagtgata tctaccagca aacgatcaat tatgtggtca gtggccagca ccctacgctt
    14821 taaggtgcta tgcttgatcg gcaacctaat ttaggggttt agcacgtgtt tcttcgctac
    14881 ggcgatgttg tccttaaaac tagcaacagg attgaggagt taaaatgaaa tcgaaccgtc
    14941 aggcacgtca tattcttgga ctggaccata aaatttctaa ccagcgcaaa atagttaccg
    15001 aaggtgacaa atccagcgtg gtaaataacc caaccggcag aaaacgcccc gctgaaaagt
    15061 aattcataac catcagtcct caatgacgat taaacaccat tgcctgcgca atggtgtttt
    15121 tgtttttatc tgctttatac ttggggccga cgccctggcg gtaaagcaaa gacgataaaa
    15181 gcgtgccagg gatggatatt caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac
    15241 agcgttcgct ttatatccct tacgctggcc ctgtactgct ggaatttccg ttgttgaata
    15301 aaggcagtgc cttcagcatg gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg
    15361 aagtggtcga aaccatcgaa gaacaagcgg aacgagcatg gatccagtat cagggattca
    15421 aaaccgaaat cgacaaacac atctacctgc gtaacatcca ggacaccaac gaaaccctct
    15481 tctaccgtct ggtaaacaat catcttgatg agatgatgcc tgttatttat accccaaccg
    15541 tcggcgcagc ctgtgagcgt ttttctgaga tctaccgccg ttcacgcggc gtgtttatct
    15601 cttaccagaa ccgccacaat atggacgata ttctgcaaaa cgtaccgaac cataatatca
    15661 aagtgattgt ggtgactgac ggtgaacgta ttctggggct tggtgaccag ggcatcggcg
    15721 ggatgggcat tccgatcggt aaactgtcgc tctataccgc ctgtggcggc atcagcccgg
    15781 cgtataccct tccggtggtg ctggatgtcg gaacgaacaa ccaacaactg cttaacgatc
    15841 cgctgtatat gggctggcgt aatccgcgta tcactgacga cgaatactat gaattcgttg
    15901 atgaatttat ccaggctgtg aaacaacgct ggccagacgt gctgttgcag tttgaagact
    15961 ttgctcaaaa aaatgcgatg ccgttactta accgctatcg caatgaaatt tgttctttta
    16021 acgatgacat tcagggcact gcggcggtaa cagtcggcac actgatcgca gcaagccgag
    16081 cggcaggtgg tcagttaagc gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat
    16141 gcggcattgc cgaaatgatc atcgcccaga cccagcgcga aggattaagc gaggaagcgg
    16201 cgcggcagaa agtctttatg gtcgatcgct ttggcctgct gacggacaag atgccgaacc
    16261 tgctgccttt ccagaccaaa ctggtgcaga aacgcgaaaa cctcagtgac tgggataccg
    16321 acagcgatgt gctgtcactg ctggatgtgg tgcgcaatgt aaaaccagat attctgattg
    16381 gcgtctcagg acagaccggg ctgtttacgg aagagatcat ccgtgagatg cataaacact
    16441 gtccgcgtcc gatcgtgatg ccgctgtcta acccgacgtc acgcgtggaa gccacaccgc
    16501 aggacattat cgcctggacc gaaggtaacg cgctggtcgc cacgggcagc ccgtttaatc
    16561 cagtggtatg gaaagataaa atctacccta tcgcccagtg taacaacgcc tttattttcc
    16621 cgggcatcgg cctgggggtt attgcttccg gcgcgtcacg tatcaccgat gagatgctga
    16681 tgtcggcaag tgaaacgctt gcacagtatt cgccgctggt gctgaacggc gaaggtctgg
    16741 tactaccgga actaaaagat attcagaaag tctcccgtgc aattgcgttt gcggttggca
    16801 aaatggcgca gcagcaaggc gtggcggtga aaacctctgc cgaagctttg caacaagcca
    16861 ttgacgataa tttctggcaa gccgaatacc gcgactaccg ccgtacctcc atctaagcct
    16921 gcgcccggta gtgaaggcta ccgggcaatt ttcctctccc tttttcagat ctcatccata
    16981 ctgggtagtg gcgaataaat ctcatttgcc tcacctgcta tgcagaacat catccgaaaa
    17041 ggaggaacta tgaaggctgc agttgttacg aaggatcatc atgttgacgt tacggataaa
    17101 acactgcgct cactgaaaca tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt
    17161 cataccgatc ttcatgttaa gaatggcgat tttggtgaca aaaccgacgt aattctgggc
    17221 catgaaggca tcggtgtggt ggcagaagtg ggtccaggtg tcacctcatt aaaaccaggc
    17281 gatcgtgcca gcgtggcgtg gttctacgaa ggatgcggtc attgcgaata ctgtaacagt
    17341 ggtaacgaaa cgctctgccg ttcagttaaa aatgccggat acagcgttga tggcggaaga
    17401 gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact cggcggcggc
    17461 cagcagcatt acctgtgcgg gggtcaccac ctacaaagcc gttaagctgt caaaaattcg
    17521 tcccgggcag tggattgcta tctacggtct tggcggtctg ggtaacctcg ccctgcaata
    17581 cgcaaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg agcagttaaa
    17641 actggcaacc gaaatgggtg cagatttagc gattaactca cgcaccgaag acgccgccaa
    17701 aattgtgcag gagaaaaccg gtggcgctca ctctgcggtg gtaacagcag tagctaaagc
    17761 tgcgtttaac tcggcagttg atgctgtccg tgcaggcggt cgtgttgtgg ctgtcggtct
    17821 gccgccggag tctatgagcc tggatatccc acgtcttgtg ctggatggca ttgaagtggt
    17881 cggttcgctg gtcggcacgc gccaggatct aaccgaagcc ttccagtttg ccgccgaagg
    17941 caaagtggtg ccgaaagtcg ccctgcgtcc gttagcggac atcaacacca tctttaccga
    18001 gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcact aagaggtctt
    18061 tgctgcgact cccatgttcg ggtcgcagca tcgcacactc tccaacatga aatggctaaa
    18121 tggattattg ttgcgtagga aggaacaata atgatagtgt tcccagagaa ggaacgctac
    18181 tgagagtaaa gacactaatg aaaatggcca atcatccccg cccgggggac attattcagg
    18241 aatcactgga cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc
    18301 cctcaacggc aagccgattg ctgaccggaa aagcagcttt gacgccagaa atggcaatta
    18361 aactttccgt ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga
    18421 gtctggcaga ggcagaaaaa acggtggatg tgtcgcgatt acgccgtctg gttacgcaat
    18481 aaaaaatggc accttataaa gcgccatttg aaactgatga caaacgcaac attgcctgat
    18541 gcgctacgct tatcaggcct acacgttcac ttcaatctat tgtatttgaa gggttttcat
    18601 gccggataag gcattcacga cgcatccggg atgaacaaag cccacgttgt ctacaatatg
    18661 aaaatggcgc cttttacagc gccattttca gcttaaagtt caaaagagat tatatccctt
    18721 cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc ggatggtgtt
    18781 tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac cctttcaccc
    18841 agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc gcagccgcgt
    18901 ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag tacggacgcc
    18961 agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac cagaacatca
    19021 tcttttgccc ggcgttgtac ttaccaacat ccgccacttt gtgctcattg ccttttaata
    19081 cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg tgatgcacaa
    19141 aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaagaac gggtgcaaaa
    19201 tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc gggaagaaga
    19261 acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag tgacaggcgc
    19321 gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc ctcctcgtca
    19381 tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc ggcaaaagtg
    19441 gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct taccgaggtg
    19501 tcgatcttcg gatctttcgg cagaccgtaa tacagctccg gctgatcggc gtgatgcagc
    19561 acgtacataa cgtgcgtacc accgacccct ttcgggttgt agacgccagc atgttcgtaa
    19621 ccacgcgctt tcagtttcgc cacgcgctct tccgccagct ccagcatctc cttcttggtg
    19681 ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg gccgacgctg
    19741 acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt gaggcgcgga
    19801 atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc cgactggaaa
    19861 tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc cttcaggcag
    19921 ccgggatctt cacagtgcat acagccgtct ttacggatca gccactccag cttgccgttc
    19981 tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc ggcggggtta
    20041 tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc cgaacacgcc
    20101 acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagttttgc gacttctgct
    20161 ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct tttgataata
    20221 tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt gactaagaac
    20281 gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag agtgttagcg
    20341 atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc caccgtttca
    20401 acctgctgac cgtttacatt cagcggtttc agacggcgcg ttaccaccgc caccgcgcgg
    20461 ataaagccac gcttgcttga gacagtgaca cgatcgccat tattaatgcc tttcgccgcc
    20521 gccagcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt gagcaatgcg
    20581 tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac atacgggaac
    20641 tgctcttttt tacccatccg cagcgcgtct tgttcataca gacgaacaac cgggttagac
    20701 accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc gtagtgttcc
    20761 gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc ttccggctgc
    20821 atgataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtccgg aatatcgtta
    20881 cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca cggtttacca
    20941 ttgatatccg ccgaagcacg gttgtagagc acgcgacggt tgagcggcca cgcccaggcc
    21001 catcccagcg tattccccag accggatggg tcggagttat cgcggttagc catctggttg
    21061 ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt accgtcatca
    21121 cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac gccattagcg
    21181 tcatagagat cttccagcgc atagccgtta ttctctttcg ccacttcgtc agattgcggt
    21241 tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc tttaccacct
    21301 tcggcctggt acagctcgcg cagatgatgg tagatacccg ccagaatttc gccgtcgtta
    21361 cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact gcaaccagcg accggagtta
    21421 gcgatagaac catcttcttc ggcaaagcag gtcgaaggca gacggaatac ttcagtctga
    21481 atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt agaggtttca
    21541 gtcaccagcg gatcgataac caccatgtac ttcagcttgc tcaggcagct caccactttg
    21601 tttttgtccg ggaaggacgc aaccgggtta aagccctggc agaagtaacc ggtgactttg
    21661 ccttcgtcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca cttcggcagc
    21721 cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga tttcatcagg
    21781 ctaacgaaga acttcggata gttgctccag tagttcacct gatcagccag cgtcgctttc
    21841 ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg aagcgtcaga
    21901 taacctggca ggctggtaga gagcagacct aagtcagtca gcccctgaat gttggagtga
    21961 ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag caactggatc
    22021 atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc cagcgcgtac
    22081 aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca cactttcagg
    22141 aagtcggctt ttggcgtacc gcagatgttt tcaacgacgt ccggcgtgta gcgggaaacg
    22201 tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc gcgttttgca
    22261 tagccgtttt catcgaactg atagttccag gacgacttat catactggcg tttttcagcg
    22321 tcgtagccgc tgaacaggcc gtcttcgaaa gcaaaatcat cacgcaccag caggctggcg
    22381 ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag gtagcgcaaa
    22441 acgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat atccgccaca
    22501 gaagcggtac gcgtaaaacg aggatcgaca acgatcaagg ttgcatcgtt gttgtttttc
    22561 gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc gcccattacc
    22621 attacgacgt tagcgttttt gatatccacc cagtggttgg tcatcgcacc gcgaccaaat
    22681 gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt gtctaccgcc
    22741 agcatcccga gggagcgggc aaatttctgg gtcagcatcc cggtttcgtt gctggcaccg
    22801 gaggcacaca gcataccggt agaaagccaa cggtttaccg ttacgccctg ctcgttcttt
    22861 tcaataaagt tagcgtcacg gtcagctttc atcagcttcg caatacggga gaatgcttct
    22921 tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatattccgg gtagcgcaga
    22981 cggttttcgc tgttgacgta atccagcaaa ccggcccctt ttgggcacag cgcaccacgg
    23041 cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc gtttttcgcg
    23101 ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt gcaggtgtta
    23161 cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc ttgcttcggg
    23221 gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat tttaaaaaat
    23281 tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg cgcgaggaaa
    23341 ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg tatgccacaa
    23401 aataatccgc ctgcgcaaat taacctacct caatagcggt agaaaaacgc accactgcct
    23461 gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc aagagagaca
    23521 aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg atcgtcctgt
    23581 ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggtccg gtcggcggcg
    23641 cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga tttccgcgta
    23701 ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc agctatgaaa
    23761 tctgtctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt gaagtgggta
    23821 tggtgaacta tctgtggccc agcctgacaa ttctctttgc cattctgttt aatggtcaga
    23881 aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc tgctgggtgt
    23941 taggcggtga gaatgggtta cattatgatg aaatcatcaa taatatcacc accagcccat
    24001 tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc acagtaacga
    24061 ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg ggagcatgtc
    24121 tgtggattta ctattttctc acgccacaac cagaaatggt atttagcacg cccgtcatga
    24181 ttaaactcat ctctgcggca tttaccttag gatttgctta tgctgcatgg aatgtcggta
    24241 tattgcatgg caatgtcacc ataatggcgg taggctcgta ttttacgcct gtactttcct
    24301 cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg caaggcgcgc
    24361 taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt taaaataatt
    24421 gccgggtcta cggcccggca gttaatcaga taaattccga caata
//
LOCUS       DAZOYS010000045        24414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOYS010000045 DAZOYS010000000
VERSION     DAZOYS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24414
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.045"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            32..748
                     /locus_tag="ACHUN9_001593"
     CDS             32..748
                     /locus_tag="ACHUN9_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0695022.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            778..1278
                     /locus_tag="ACHUN9_001594"
     CDS             778..1278
                     /locus_tag="ACHUN9_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0695023.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1370..4054
                     /locus_tag="ACHUN9_001595"
     CDS             1370..4054
                     /locus_tag="ACHUN9_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0695024.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4051..5139
                     /locus_tag="ACHUN9_001596"
     CDS             4051..5139
                     /locus_tag="ACHUN9_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0695025.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5222..6519)
                     /gene="exuT"
                     /locus_tag="ACHUN9_001597"
                     /pseudo
     CDS             complement(5222..6519)
                     /gene="exuT"
                     /locus_tag="ACHUN9_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            7002..8414
                     /gene="uxaC"
                     /locus_tag="ACHUN9_001598"
     CDS             7002..8414
                     /gene="uxaC"
                     /locus_tag="ACHUN9_001598"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR0695026.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8429..9916
                     /gene="uxaA"
                     /locus_tag="ACHUN9_001599"
     CDS             8429..9916
                     /gene="uxaA"
                     /locus_tag="ACHUN9_001599"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0695027.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9999..10550
                     /locus_tag="ACHUN9_001600"
     CDS             9999..10550
                     /locus_tag="ACHUN9_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0695028.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10555..11799)
                     /gene="sstT"
                     /locus_tag="ACHUN9_001601"
     CDS             complement(10555..11799)
                     /gene="sstT"
                     /locus_tag="ACHUN9_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0695029.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12415..13380)
                     /gene="alx"
                     /locus_tag="ACHUN9_001602"
     CDS             complement(12415..13380)
                     /gene="alx"
                     /locus_tag="ACHUN9_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0695030.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13663..14649)
                     /locus_tag="ACHUN9_001603"
     CDS             complement(13663..14649)
                     /locus_tag="ACHUN9_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0695031.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14728..15411)
                     /locus_tag="ACHUN9_001604"
     CDS             complement(14728..15411)
                     /locus_tag="ACHUN9_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0695032.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15488..15991)
                     /locus_tag="ACHUN9_001605"
     CDS             complement(15488..15991)
                     /locus_tag="ACHUN9_001605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0695033.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16076..17212
                     /gene="rlmG"
                     /locus_tag="ACHUN9_001606"
     CDS             16076..17212
                     /gene="rlmG"
                     /locus_tag="ACHUN9_001606"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0695034.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17497..17811
                     /gene="higB"
                     /locus_tag="ACHUN9_001607"
     CDS             17497..17811
                     /gene="higB"
                     /locus_tag="ACHUN9_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0695035.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17808..18224
                     /gene="higA"
                     /locus_tag="ACHUN9_001608"
     CDS             17808..18224
                     /gene="higA"
                     /locus_tag="ACHUN9_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0695036.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18269..20287)
                     /gene="fadH"
                     /locus_tag="ACHUN9_001609"
     CDS             complement(18269..20287)
                     /gene="fadH"
                     /locus_tag="ACHUN9_001609"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0695037.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20513..22864)
                     /gene="ygjK"
                     /locus_tag="ACHUN9_001610"
     CDS             complement(20513..22864)
                     /gene="ygjK"
                     /locus_tag="ACHUN9_001610"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0695038.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22881..23958)
                     /gene="ygjJ"
                     /locus_tag="ACHUN9_001611"
                     /pseudo
     CDS             complement(22881..23958)
                     /gene="ygjJ"
                     /locus_tag="ACHUN9_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24092..>24414)
                     /locus_tag="ACHUN9_001612"
     CDS             complement(24092..>24414)
                     /locus_tag="ACHUN9_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695039.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6168 a         6197 c         6256 g         5793 t
ORIGIN      
        1 tttggaaaca atattgtaag atcaaattta tatggtggcg aaaattaatc tcttatcacg
       61 gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata tgactgttta
      121 tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag
      181 taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat cgacgcctca
      241 ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta tgccacctaa
      301 attcgcttta ccagccggta gtagcaaaac tgtgcgcttt gttgcaatgg aaccagagca
      361 aaaagaaaaa aattatcgcg ttaaatttga agcggttccc agtattgatg acgttgccac
      421 agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga ttgttgttag
      481 tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa aattagttaa
      541 tgcagggaat caacgcttaa agattttaac gatcgcttat tgtaaaaata atagcaaaga
      601 aaattgtaag atacagaccg taaataaaaa catcttcctt ggtcaggaaa gaaatcttga
      661 aagcatatct ggctacgaca aaatcgttgt caaatataat aactggatca ccaaagataa
      721 tggcgagttt gaactggcgg tccattaatt atcattgtaa taaacgaagg aatagtaatg
      781 aaaaaggtat ttgcaaaatc tcttctggtc gcagcgatgt tttctgttgc aggctccgca
      841 ttggcagtac aaaaggatat tactgtaacg gccaacgttg atgccgctct ggatatgaca
      901 cagaccgata acaccgcgtt gccgaaagca gtagaaatgc aatatctgcc ggggcagggt
      961 ctgcaatctt accagttgat gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa
     1021 atgcagttag tttccccggc gcaactggtt caaagcctgg atgctagcaa gattgttccg
     1081 ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac ttttaccgca
     1141 accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa cctgatgttt
     1201 gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt aagcatttat
     1261 ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg aggggtagcc
     1321 ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca tggataaaaa
     1381 attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat taaccgaaat
     1441 tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg tttatttttc
     1501 agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa catttcttga
     1561 tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa tcgcgcatat
     1621 agtgaaagaa aaattatcgc agccgctagc tcgccacggt gaattggctt gcggttatgt
     1681 acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa tctataatga
     1741 tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa
     1801 gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc agcaagacat
     1861 caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg gtgcgctggg
     1921 aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg atgcagatga
     1981 tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt ttttacgtcg
     2041 ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac aaggcgggaa
     2101 ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg ggtcgacact
     2161 gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc tgctttcgcg
     2221 caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt atctcaatag
     2281 tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg tggcattaaa
     2341 agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg
     2401 tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat cacaggtttc
     2461 tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc cgcaatatga
     2521 gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag gtaataactg
     2581 gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt tccgtaacga
     2641 tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat ggccgacgtt
     2701 gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca gagagagcta
     2761 taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg
     2821 gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt tgcgttggga
     2881 taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa gtatctctga
     2941 aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc
     3001 agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg gcttgtactt
     3061 atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc attcaacaaa
     3121 tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat ggcagttatc
     3181 gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg tcggaggcct
     3241 gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt
     3301 ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt cggcctttac
     3361 gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg gagccagtgg
     3421 tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg aacaggatga
     3481 agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg
     3541 tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg ttgaggttaa
     3601 cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg cagggaatcg
     3661 tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta
     3721 caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga ttgtggggct
     3781 gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa ttaacggcag
     3841 cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac tgagtgttgt
     3901 aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg tgacttattc
     3961 acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat tacgtggaaa
     4021 cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc ggcgatattg
     4081 ggcttgtttg gcacgctcat tggcgttcag gcagctcctg acgtgaccag tgaaattacg
     4141 tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc gtcggcagga
     4201 aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac ctgtacagct
     4261 aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg gatgcgttgg
     4321 ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc gccgccagga
     4381 tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc ttcatgtaca
     4441 gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta ccttgagcag
     4501 acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa attacattcg
     4561 aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga ccccaaccat
     4621 attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt agacttgcat
     4681 ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc
     4741 ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa agatgaaggg
     4801 cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg agggaccacc
     4861 accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga aaccggtggg
     4921 caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg
     4981 cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac acccttgact
     5041 ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat gggcaaattg
     5101 tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcagacc agaaaaaact
     5161 aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta aacgttaaac
     5221 gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc gccggtttgt
     5281 tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc agaactgcga
     5341 acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca aacagcgtac
     5401 ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga
     5461 acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca aaaccaccga
     5521 tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata cccgggccaa
     5581 tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa
     5641 accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg tcagcaaaca
     5701 gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca
     5761 tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg aaacgcggca
     5821 gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgccg
     5881 tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct tcatcggtca
     5941 gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc
     6001 tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt gccataccac
     6061 cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc ctactgcgat
     6121 ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc
     6181 tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc tacctgccag
     6241 cgcggttaca ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt
     6301 acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag cagcagaata
     6361 ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg
     6421 cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag
     6481 tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt
     6541 atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg
     6601 gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg
     6661 cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg
     6721 tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag
     6781 ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg
     6841 gcgcggcctg cagcgagatt tatcactttg agagtaattt ttttaactac gtttattgat
     6901 ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc
     6961 accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg
     7021 aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc
     7081 agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt
     7141 ttaaaaacct gtatgacatc tggctgaaag gcgatcacta caaatggcgc gctatgcgta
     7201 ccaacggtgt ggccgagcgt ctgtgtaccg gtgatgcatc tgaccgtgaa aaatttgacg
     7261 cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg acgcaccttg
     7321 aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa
     7381 tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc
     7441 agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc
     7501 acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg
     7561 acaaagcctt caacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag
     7621 tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg
     7681 atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg
     7741 ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg ccgtctggct ggcgaaaccc
     7801 tgagcgagca cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat
     7861 atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc
     7921 gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg
     7981 cggaagagtt gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca
     8041 ttctctactg cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc
     8101 agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga
     8161 aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtctgctg agccgctttg
     8221 tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc
     8281 gcattctgtg ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca
     8341 acctgctggg cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca
     8401 ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa
     8461 cgtcgcggtc gctttagcag atttggctga aggcacagaa gtcagtgtcg ataaccagac
     8521 tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa
     8581 aggggccaac gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc
     8641 gggggaacac gtccacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg
     8701 ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta
     8761 tcgtcgcgct aacggcgatg tcggggtgcg taacgagctg tggatcctgc caaccgtggg
     8821 ctgtgtcaac ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc
     8881 cgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac aactgggcga
     8941 cgatcacatc aacacccgca ccatgctgca aaacatggtg cgccacccga acgcgggcgc
     9001 agtgctggtg attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt
     9061 gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat
     9121 cgaagccggg atcgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga
     9181 gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc
     9241 tggtattact gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg
     9301 tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca
     9361 ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact ttaaacagta
     9421 ctttattgcc catgatcagc cgatctacga aaacccatca ccggggaaca aagcgggcgg
     9481 tatcaccacg ctggaagaca aatcacttgg ctgtacccag aaagcgggtt ccagtgtcgt
     9541 ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgcct gggctgaact tgttaagtgc
     9601 gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct
     9661 gttcagtact ggtcgcggca cgccatacgg tggatttgtg ccgacggtga aaatcgccac
     9721 caacagtgaa ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat
     9781 ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc
     9841 caacggtaag caaacttgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag
     9901 cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga
     9961 agcgccgttt gcttttccat acctgataag gatttatcat gaccgcgtat tggctggccc
    10021 agggcgtggg tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac
    10081 gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc
    10141 tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct
    10201 tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc
    10261 tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct
    10321 gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt
    10381 gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacaatgatt gagggtagtt
    10441 ttctgcttat gaatggcttg aatatcattc gtttctggcg gatgcagaaa aggggaattg
    10501 atccgtttaa agtagagaaa acccctcccg ccgtagacga aaggggttaa acaattaatt
    10561 acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag
    10621 cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa
    10681 gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag
    10741 cgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac acagagaagc
    10801 caccacgctc aacagcagcg ccgtgggcag atcgaccggg atacccagcg tattaaccgc
    10861 agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggcacccag
    10921 cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca gtgccatatt
    10981 caccggaatg ttcgccgcag agctgcgggt gaagaaggca tacacgccgc tttcgcgcag
    11041 gcacagcagc accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt
    11101 aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc
    11161 ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccaat
    11221 cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac
    11281 caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac
    11341 caggatcccg atgtagttac ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat
    11401 taccagcccg cgcatcactt ctacaatgcc tgacggtggc gaaatatcac ccgcgctact
    11461 ggataagtgc agggtagaag ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga
    11521 gaaggtaccc agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg
    11581 gtggttagca atagatgcca tcaccagcat caacaccagg atgggggcaa cggctttcag
    11641 tgcgccgacg aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgatatcca
    11701 tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc
    11761 cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt
    11821 tatcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc
    11881 aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag
    11941 aagtcatatt ttgaaactta atggttacat ttgtttgaca taaacagagg gaaggactgg
    12001 ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag aacggttgcg
    12061 ctcctttaat gtgctatgcc atataaagtc actggcgcag ggagcgcgca gggggcggcc
    12121 aatcgccgcc gccccctgca cccccaggct ctggcgaaca aaatcgccgc tgcgcgattc
    12181 cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct gtaagatgcg
    12241 ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg gcgattttga
    12301 cgccaccaac acccgtgcgg tctttttgta aagagctaca agccgattac ataattaata
    12361 gctcaccttt gtctggaatg agttatctct aggaatggct taatatacag cgaatcattc
    12421 tccccgctgc ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag
    12481 aatgccaaac accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag
    12541 catcttgata ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg
    12601 ctctgctacg cccgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt
    12661 tgaggtcagc acaatgaacg ggtcagtggt cacggcgaag atagccggaa tgctatccac
    12721 ggcgaaaatc acgtcgctca attccaccag aatcagtacc agcatcagcg gtgtggcgta
    12781 cagcaatcca ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa
    12841 atgaccgcgt agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc
    12901 gagcgccatc ttcacgccgg taaacagcag gaaggcaccg aagatataca ggatccagtc
    12961 gaactgcgaa attagccagc tgccagtgaa gatcatgatg gtacgcagaa caatcgcccc
    13021 gagcacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa
    13081 caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacccgt
    13141 gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcagtttgca ccagatacca
    13201 ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc
    13261 ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag
    13321 cataatggcg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat
    13381 agaagttcct tacacataaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta
    13441 tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaaggaat acgtctgcgt
    13501 attcctttcg ggttgcccgg tgaccggatg tggtttttca cacatcgtaa tgacgatcga
    13561 ccggcaatga agttactccc ctttgcaggt aacaaagtat gacagaccat tcagccagtc
    13621 aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt ttacgctatc
    13681 tgccggaaaa atcaccccag tctggcggcg gatctcagtc agcagtttgg cggtgatgcg
    13741 actgaccgcc agccctggat gatccaccag atgctcatcc accagggtag caaacagctc
    13801 tgcttcgtag agcatggtat taatatgctg cggctgggtg agatcctgca tctggctgcc
    13861 acgcggcacg aagcacactt tctggcattc agacagtttt tcaatcacca gcgatcccgc
    13921 ttcgccctga atctcgcttt ccaggacaga atcactgacc tttgagtgct gcaacgtaac
    13981 gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tctacgccgc ttgccaacaa
    14041 actggcggtt gcctgcacgc ttttcggttc accgaataat gccaccgccg acgccaggca
    14101 gtaaaagccg atatccataa ttgaaccgtt agagaatgcc ggattaaagg tgttgggatt
    14161 ctcaccatcc aggtaacgtt gataccgcga ggaatactgg caatagttga aaaagacttt
    14221 acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg gcaggctggc
    14281 ggttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa tggcggcatc
    14341 cacttccgcc agattcgacg ccagtggttt ctcgcaaatc acatgaattt tatggctaag
    14401 gaaaagttgt gtctgggaaa agtgcagggc attcgggctg gcaatataca ccgcgtcaat
    14461 ggcatcgctt tccgccatcg cttccagcga ggtaaacaga tgctcgacag aaaaatcatt
    14521 ggcgaagtgc tgggcctgtt caaggctgcg ggaatatacg gcggttaact tgtatttacc
    14581 gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa tcacagcgaa
    14641 acgtatcata acgcgtggat actccataaa gggattctgt cgtcaatgta gcacgccttt
    14701 ttcatcttcg acgtgcgcag atacgcatta ttctttcgcc ggacagccat gctcactaaa
    14761 cggcccaatc atgaccgatg gcccgagata tttcggctgg cgatggagga ttttggcgtc
    14821 aatcaccgcg ctgacacgag cgagtttctc ccgcagttga gtataaaaac cgtgcttcat
    14881 attaaggtct ggcgcgttaa acccgatagc gtcgatgccg tactgttttg ccagccagat
    14941 ggcgcgttgg ttatgaaatt cttgcgagat aatggtgata tggttttccc caaacacctt
    15001 tttggcacgc accactgaat caagcgttga gaatccggca tagtcgcaga agatcacttt
    15061 cgccggtacg cctttagcga tcaaagcctg ctgcatacct gaggcttcat cgtaattttt
    15121 acgtccgtta tcgccactga ctaacagcca tttcactttt ccgacgtggt acaacgctgc
    15181 tgcggtatca atacgtcggg taaagtagcg attacccggc ctcgccccca gcaataaacc
    15241 cacgttacgc gccggaactg cgttgacatc gctccaggtc agctgcttgc tggcgttgac
    15301 cattacgcga tcggcgataa aaatggtcgc tcctgccaca agtaacagta agcaggtaat
    15361 ctttagagtc cggcgagaaa aacaaatacg gagaagaagg cggggctgca tggtcgttat
    15421 ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac
    15481 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    15541 actcaagctg gtggtactgc ggctccatat ggcagcacaa ctggtaaaac gctttgttat
    15601 gctctttctc cttcaggtgc gccagttcgt gcaccacgat catgcgtaaa aacggttccg
    15661 gcgcgttgcg aaacacagta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    15721 gcacacgaga aacagcggta tgtaatccga gcgcgttttt cagtacgtgg atcttattgt
    15781 catacatcac tttagtgatc ggcggggcat tgcgcagaaa ctgatttttt agatcctggg
    15841 tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    15901 gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt agctgctcgg
    15961 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    16021 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    16081 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    16141 taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt tggacgacac
    16201 agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    16261 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    16321 gaatttacgc ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    16381 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    16441 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    16501 caaagcccgt gacattcaca cttccacgct ggagctgttc gagaaagtgc tcggcccgac
    16561 caccactacg ctggcatgga aaaaagcgcg cctgattaat tgcactttca atgaaccgcc
    16621 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    16681 caaccatgcg aatgtcttct cccgcaccgg gctggatatt ggcgcacgct tctttatgca
    16741 acatctgcca gagaatctcg aaggtgagat tgtcgatctc ggttgcggta atggcgttat
    16801 tggtctgacg ctgcttgata aaaaccctca ggcgaaagtg gtgtttgtcg atgaatcgcc
    16861 gatggcggtt gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttagatcg
    16921 cagcgagttt atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt
    16981 gctctgcaac ccgccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat
    17041 gttccaccat gcccgccgct gcctgaaaat caacggcgag ctgtatattg ttgccaaccg
    17101 tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca ccatcgccac
    17161 gaataataaa tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt aagattggga
    17221 gttacctgcc ggatgcggcg ttaacgcctt atccggccta cgttcagcac gttactccca
    17281 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc tggaatcgcg
    17341 cttccataaa acaaaaaatc cctctcacca tcctctacat tctcttgttt agcgtttttc
    17401 tacgtttatt cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt
    17461 atcatattgc caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca
    17521 ttgaaagatg ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac
    17581 acgattgcta agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg
    17641 gataacttca aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt
    17701 gttgtagcaa tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat
    17761 aaagaatacg atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg
    17821 ccgacatctt gcaagcagga gaaaagctaa ctgcggtggc accttttctg gcgggtattc
    17881 agaacgagga acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc
    17941 ctgaaaaccc cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc
    18001 ccgaatttgc ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc
    18061 gtacccttat ggatcaatat ggtttaaccc ttgccgatct gccggaaatt ggcagtaaat
    18121 ctatggtgtc acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat
    18181 tggcaacgcg attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg
    18241 cgttgcatcc ggcgcttaaa ataacgattt aaatctccag cgccagccgt gttccctggg
    18301 caattgcccg tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag
    18361 ttttcccgct atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga
    18421 tcaccacatt gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt
    18481 catcgtcaat cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg
    18541 tggtgcgatg gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc
    18601 gctggagcat cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc
    18661 caccagcctg ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct
    18721 ggctggtgga ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac
    18781 aaccgatgat ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca ttgagataac
    18841 tcaatacctt cggatgatcg atcccgtcga taggcggaac gcgcggcacg atcccactgg
    18901 cgaggatcgt ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca
    18961 gcgtcacgcc cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg taaaactcct
    19021 ctttgccggg gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgggcatcaa
    19081 acaatgttac ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc
    19141 ccgcaccgac caccgccaga ttttttttct gcacggcagg aaggattggc attttggttt
    19201 cgtggcaggc gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga
    19261 ggcaggcctg attgcagcca atgcaggtgt tgatctcatc ggctcgcccc gattgcgctt
    19321 ttgacagcag ttccgcatca gcaagaaacg gtcgtgccat cgataccata tcggcatcgc
    19381 cgcgcgagag aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg
    19441 gcagcgagac gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg
    19501 gcgtggcaat ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg
    19561 cgcccgccgc ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt
    19621 cgaccaggtc gagcatcgac agtcggtaga taataatgaa gtcgttgccg acgcgttcgc
    19681 gcaccgcacg cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact
    19741 ggtcactacg ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggacc
    19801 ccattacctc tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat
    19861 cgtcgatcag ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg
    19921 gggcctgcaa tgcggaggga gcgaccagat gcggttggta gctgtagcgc ccggtatgca
    19981 aaatttgcag ggctattttg ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt
    20041 gtgggatctg gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat
    20101 ctggtgcaat gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa
    20161 acgctgccag ccgctcggca ccgtccgggt attcctccag cccggtgtgc attgagccca
    20221 tcaacacgcg gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt
    20281 agctcataaa attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt
    20341 agccgtcgat ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga
    20401 atacgcccgg tggtaacgcc ggatgcaacg cttgccgcgt tttatccggc ctgggggatt
    20461 gcacatgtag ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct
    20521 tacggaaaaa atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg
    20581 cgccttgctg tgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta
    20641 atcctttggc gtgccggaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt
    20701 aacgctccat ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccacgccagt
    20761 agatatcagc gccaaaagcc ggattggtta acgccgccgt tcccagcggg acaaaggtgt
    20821 tgaactcttt aggatcgagc atcaccttca ccaccgcgtc ggcgttggcc tgcgttgccg
    20881 caccgttaaa cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc
    20941 ccgcgcagcc gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag
    21001 tcgggtcgaa catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc
    21061 gtttggcctc ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc
    21121 tgtacatata gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc
    21181 cgtcctgact gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag
    21241 cgacatattt atccagctgt tctttgtcga taaacccaaa gacggcggca tcatcacgcc
    21301 ccgattccca cgatgcagca acctgtgccg gaatttccag actgtcgtac aggcctttct
    21361 ccaccacgcg ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca
    21421 ccgtaaacag catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt
    21481 ccggcacgcc gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca
    21541 gtttcgggta catctctgcc aaccaggctt tatcctgggt gacgttgtac acttccatca
    21601 ccgaccaggc ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac
    21661 cacgctcggg gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac
    21721 gcacgctatc gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga
    21781 tgtccggatt gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct
    21841 gattgccgga gaaccagcgc ccggtcaccg acggtgtaac ggtgttaaat ttcaccgcac
    21901 cgcccggtga gagccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct
    21961 gttccggcgt cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct
    22021 gcgaggcggt gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt
    22081 tgctaacttc ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt
    22141 tgatatgcgc cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt
    22201 tatgcacctg atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc
    22261 caaaggtgac tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt
    22321 cgccagcaat ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca
    22381 gttcgccgtc ccacaccaga tccagcggtt tattgctggt gattttggtt tccagtagtg
    22441 acgtgcgcgg cgtggcgaag cgcagagtca tttcgacttg cacatctttt gctgtcagtt
    22501 tttgcaccag cgcaccggga atactgtatg cctccagcgt aaagtcgact ttcttgccgt
    22561 cctgccagac ggtcaggcgg tcgaaattgc tggccataaa gttgatgtac tcttccgtca
    22621 gcagcgcaac gcccggaaag ccgcccatgg tgttagggcc gtctggcaac agatgaccat
    22681 gccaggctcc gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct
    22741 tcatgtactg cggcgcgcca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg
    22801 catgggcgga aaaacttatc agcagagcgc aggttactgg cgttaaaata gtttttattt
    22861 tcatgaaatt acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg
    22921 ggatcgtgag aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg
    22981 ccgccgtatt cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg
    23041 tcagggcgat gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca
    23101 tacatggcgc tcaccatcat cccactttca cttcccggta cgtccagccc gtcggcgcgt
    23161 ccggtatggt gccaggcgcg agcggcaaag ttaggagtta gcaggcccgt gagatagagc
    23221 tgtcctgtgc cgtcggtgat ttccagaccg ttcatccagg tgatgccttc ggcaaggtca
    23281 tactggatgt aaccgttcat cattgccgga taggtgtatt tgtcgtcata gcgatcgtat
    23341 ttgccaaaat gtagccaggc tttgctttca tcatgatgtc cggcaggagt cgcggtgacg
    23401 ctataacgaa agttgccgct taagttctgt accttcagcg cagtcataat gtcgcgggta
    23461 ttgggaatga cgtagcccag atcgggggtg aaatcgcccc accattgcag gtcatccagc
    23521 gaggaatcct gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca
    23581 tagaaacggt taattccgcc ctcgaaacca ccccagctat ggtctggcac ccaggcattg
    23641 ccgtcgtggt cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg
    23701 tacttcatct ccaggccacc ttcaatatag gtaccgtcgc tgtatttatg tttattctca
    23761 ccctctaaat ccatatatcc accaccccct aatccctaat tcgccgtaaa aacgaaatga
    23821 aggagaagat gtcgaggtaa ttgcgggtgt ttttgtgtcg tcatgttctg ccggacgtgc
    23881 ttcttctcct gccgcagaaa aggcgtagca aaacggcaga gcaagtaatg ctctgcatgg
    23941 tgcagtgatt aatttcattg tgttaatcca tgagctaaag aaaaagtaca gccttttatc
    24001 gttgtaatta taaaaatata aaggcagaaa ctattttccc tctcctcatc caggaagagg
    24061 gaaaatgcct gctcattttt tattattctg cttaatcaac attgctggct ggcgtcgctt
    24121 caatatggcg ctcttcagcc gttaatccct ttatatattt actgtatttc caccacgcaa
    24181 agccaaggaa gataacaata ccgccgacgt tataaaaaat gatggtcaga atattcgcgc
    24241 cagtcgggaa tgtcgaagcg acaaacccta cggcaaatat cgcaatcagc attgaaacaa
    24301 cgataatacc ggtgcggcgg gagcccatac ggaaatcgcg tggcaggtga tctaatttgg
    24361 cgcgtaaatt caggtaagcc agcatgatga ataacggcgg aagcatggac gctg
//
LOCUS       DAZOYS010000046        24193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOYS010000046 DAZOYS010000000
VERSION     DAZOYS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.046"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            77..214
                     /locus_tag="ACHUN9_001613"
                     /pseudo
     CDS             77..214
                     /locus_tag="ACHUN9_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(322..1557)
                     /locus_tag="ACHUN9_001614"
     CDS             complement(322..1557)
                     /locus_tag="ACHUN9_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0695040.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1704..1780)
                     /locus_tag="ACHUN9_001615"
     tRNA            complement(1704..1780)
                     /locus_tag="ACHUN9_001615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1744..1746),aa:Pro,seq:tgg)
     gene            complement(1823..1909)
                     /locus_tag="ACHUN9_001616"
     tRNA            complement(1823..1909)
                     /locus_tag="ACHUN9_001616"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1873..1875),aa:Leu,seq:cag)
     gene            complement(1930..2005)
                     /locus_tag="ACHUN9_001617"
     tRNA            complement(1930..2005)
                     /locus_tag="ACHUN9_001617"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1970..1972),aa:His,seq:gtg)
     gene            complement(2064..2140)
                     /locus_tag="ACHUN9_001618"
     tRNA            complement(2064..2140)
                     /locus_tag="ACHUN9_001618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2104..2106),aa:Arg,seq:ccg)
     gene            complement(2243..3628)
                     /locus_tag="ACHUN9_001619"
     CDS             complement(2243..3628)
                     /locus_tag="ACHUN9_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0695041.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3819..4559)
                     /gene="wecG"
                     /locus_tag="ACHUN9_001620"
     CDS             complement(3819..4559)
                     /gene="wecG"
                     /locus_tag="ACHUN9_001620"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0695042.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4562..5914)
                     /gene="wzyE"
                     /locus_tag="ACHUN9_001621"
     CDS             complement(4562..5914)
                     /gene="wzyE"
                     /locus_tag="ACHUN9_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0695043.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5911..6990)
                     /gene="rffT"
                     /locus_tag="ACHUN9_001622"
                     /gene_synonym="wecF"
     CDS             complement(5911..6990)
                     /gene="rffT"
                     /locus_tag="ACHUN9_001622"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0695044.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6987..8237)
                     /gene="wzxE"
                     /locus_tag="ACHUN9_001623"
     CDS             complement(6987..8237)
                     /gene="wzxE"
                     /locus_tag="ACHUN9_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0695045.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8239..9369)
                     /gene="rffA"
                     /locus_tag="ACHUN9_001624"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8239..9369)
                     /gene="rffA"
                     /locus_tag="ACHUN9_001624"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0695046.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9374..10048)
                     /gene="rffC"
                     /locus_tag="ACHUN9_001625"
                     /gene_synonym="wecD"
     CDS             complement(9374..10048)
                     /gene="rffC"
                     /locus_tag="ACHUN9_001625"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0695047.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10026..10907)
                     /gene="rfbA"
                     /locus_tag="ACHUN9_001626"
     CDS             complement(10026..10907)
                     /gene="rfbA"
                     /locus_tag="ACHUN9_001626"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0695048.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10926..11993)
                     /gene="rffG"
                     /locus_tag="ACHUN9_001627"
     CDS             complement(10926..11993)
                     /gene="rffG"
                     /locus_tag="ACHUN9_001627"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0695049.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11993..13255)
                     /gene="wecC"
                     /locus_tag="ACHUN9_001628"
     CDS             complement(11993..13255)
                     /gene="wecC"
                     /locus_tag="ACHUN9_001628"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0695050.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13252..14382)
                     /gene="wecB"
                     /locus_tag="ACHUN9_001629"
     CDS             complement(13252..14382)
                     /gene="wecB"
                     /locus_tag="ACHUN9_001629"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0695051.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14438..15484)
                     /gene="wzzE"
                     /locus_tag="ACHUN9_001630"
     CDS             complement(14438..15484)
                     /gene="wzzE"
                     /locus_tag="ACHUN9_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0695052.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15496..16599)
                     /gene="wecA"
                     /locus_tag="ACHUN9_001631"
     CDS             complement(15496..16599)
                     /gene="wecA"
                     /locus_tag="ACHUN9_001631"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0695053.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16839..18098)
                     /gene="rho"
                     /locus_tag="ACHUN9_001632"
     CDS             complement(16839..18098)
                     /gene="rho"
                     /locus_tag="ACHUN9_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0695054.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18183..18284)
                     /gene="rhoL"
                     /locus_tag="ACHUN9_001633"
     CDS             complement(18183..18284)
                     /gene="rhoL"
                     /locus_tag="ACHUN9_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0695055.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18425..18754)
                     /gene="trxA"
                     /locus_tag="ACHUN9_001634"
     CDS             complement(18425..18754)
                     /gene="trxA"
                     /locus_tag="ACHUN9_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0695056.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18885..20150
                     /gene="rhlB"
                     /locus_tag="ACHUN9_001635"
     CDS             18885..20150
                     /gene="rhlB"
                     /locus_tag="ACHUN9_001635"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0695057.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20284..21768
                     /gene="gppA"
                     /locus_tag="ACHUN9_001636"
     CDS             20284..21768
                     /gene="gppA"
                     /locus_tag="ACHUN9_001636"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0695058.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21815..23836)
                     /gene="rep"
                     /locus_tag="ACHUN9_001637"
     CDS             complement(21815..23836)
                     /gene="rep"
                     /locus_tag="ACHUN9_001637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0695059.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24053..>24193
                     /locus_tag="ACHUN9_001638"
     CDS             24053..>24193
                     /locus_tag="ACHUN9_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695060.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6072 a         6636 c         5991 g         5494 t
ORIGIN      
        1 tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt gatgacatga
       61 aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc
      121 cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcacgcaa
      181 aggctttgcc ggatacgatc acccaaacca gtatccacag aacgctgcaa tttattgaat
      241 ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc aaccgcaggc
      301 ggccacaaaa tggccgcttt tttacttatt caccaccagc aaatgtgcct gcataatgtc
      361 actggccggg cgaccgtgcg ccagcaaatc agccattgct ttaagatatg gcggtagatg
      421 gcggaaataa cgctgatacc cggcacacaa ataattcagc cccggtttgc cgctggcatc
      481 gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt tacaactgcg
      541 acactgcgcc ggtaattgct taaatttatc ttcaccaaac acctgctgtt gcggggaatc
      601 gatcatttct gcaattgttt gctgatgcat attccccagc cgatattgcg gataaacata
      661 gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg
      721 ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc attcgatatt
      781 catcacgaaa atcttgccga cgtcgcgttt gatccagtgg tcgaatatcg ccaccagaaa
      841 ttcaccaaat tcatcggggc gcaccgacca ttccgtcagt tccccctgaa tatcaccagg
      901 cgcatgcaat ttcagtccat cgcgggcagc tgtttcatca gccagacgct cgaccaccgg
      961 aataaactgg atgaattcga ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg
     1021 ctgcgcgctg gtgcggttga cgcagaccag cacgttatag tcgacatgat gtttttgcag
     1081 cagcgtcagg gcacgcatca ccagcttatg cgttggtctg ccacctttgg tcacgcgata
     1141 ttgattgtgg atctcagccg gaccgtccag cgataaccca acaagaaaat gattttctgc
     1201 cagaaatgca caccatttat catcgagcag cacgccgtta gtctggaagc tgttacttat
     1261 cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca cggcggtaaa aatccagccc
     1321 gagtaacgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgcg gttcgctggc
     1381 agcgatatag tgacggacat acgcttccag cgtgtcatcg tccatatgcg tgactggctt
     1441 ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca gattacaatc
     1501 ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt
     1561 cactccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc gtggggcaat
     1621 gagttattac gggcgtttat tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac
     1681 cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc cgacccactg
     1741 gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct ttttgaattt
     1801 ttagttcaat tctttaaagt cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa
     1861 ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca ttgtcacaac
     1921 ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc acaatccagg
     1981 gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat tactaccacc
     2041 gcagctcaag cgccgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc
     2101 ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg
     2161 gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg cgcgtttggt
     2221 tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg tggcgattaa
     2281 ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca acaaacagcg
     2341 acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg
     2401 ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca atcgccgctt
     2461 tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc accatccccg
     2521 gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt
     2581 tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca actggtacgc
     2641 cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga
     2701 gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca
     2761 caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg
     2821 ggctgccgtt gctgccaatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc
     2881 ctacgtagaa aatcaggatc cgccacagca ctttgcctac agcactgcgc agcgtcacct
     2941 gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca
     3001 ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct
     3061 ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag aaaatcacgc
     3121 ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact
     3181 cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc gccaccgcga
     3241 tcaatgcagg tatccactgc gccatctccg ggaaccagaa ctggacataa acgccaatgg
     3301 cggtgatttc tgagatcccc accgccatcc acataaacca gtaagaccag gcggtgagat
     3361 aaccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaacaggtt
     3421 caaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaacagc ccggcgatga
     3481 tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc cccataaaca
     3541 ggccgacgcc aatagtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac
     3601 gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg
     3661 tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga
     3721 aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga
     3781 cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc
     3841 cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg
     3901 cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg
     3961 gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg caccagacgg
     4021 cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct
     4081 ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc
     4141 tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt ttgcgccagc
     4201 acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc
     4261 agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta
     4321 cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc gttaattaac
     4381 tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat ggcaaccagc
     4441 gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg catatcacgc
     4501 caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt
     4561 gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc
     4621 tttcaaaaag ccagtacagc agttttgcga ttatcagaca tgcgccgaag accacgataa
     4681 aaaagactac gcgtgagaca aacgaatcca gcccttcacg cgccagcacg atcatattga
     4741 agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta ggctcgcggt
     4801 tgcccagctc atacagccag tcgaaccatt tgatgatcag accaaccacg atcgccccga
     4861 gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg
     4921 ccagtccgga gtggttattc agcacttccc aggtaaagta gttggctgag ttcagcacca
     4981 tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg cggacaattg
     5041 gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc
     5101 acggggagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca tcgccgctca
     5161 cattcattcc atagcgtttt agcgccatcc agaacatgcc aacaatcccc agcacgcccg
     5221 ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag
     5281 cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc agcaagccaa
     5341 aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc agaaagtaga
     5401 ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg ccggagactt
     5461 cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg ttgtgcatga
     5521 agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta agattggtct
     5581 ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc
     5641 gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca
     5701 acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg
     5761 ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc
     5821 gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag agcagccaga
     5881 ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatcgccaa
     5941 cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccac
     6001 cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa
     6061 cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc ggttaagcac
     6121 acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct ggcgggcaaa
     6181 aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa attccagttt
     6241 ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct gacgaacttc
     6301 ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc
     6361 gccaaattgc tgatgaacgg cgcgcaaggc agcaatatgg tcattgctgc ggtcaccgga
     6421 gttccccacc agaatggtca ttttcccttc acgttgccga tcattcgcca tcgtattgag
     6481 agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg gatgcgtttt
     6541 ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag ccgactcgct tttgcgccag
     6601 gcgacgtagc gggtaaaaaa gcttatatct caagccactg gaaagctcat acaggtctgc
     6661 cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca gagccagcca
     6721 cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt
     6781 cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccgg
     6841 acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct
     6901 cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt gagggatatc
     6961 cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca
     7021 caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc gcccagtgca
     7081 ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg gctgacttcc
     7141 gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa gacataagcg
     7201 cccactttta acacatcacc cactaactgc caggcaaaga gatcgcgcat agcggtaaat
     7261 ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt aaaactcgcc
     7321 gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt gatatcgcgc
     7381 ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat
     7441 tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc atcccagcta
     7501 tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta
     7561 atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccagctgggt
     7621 ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc aggaatcacc
     7681 accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc caaacggtaa
     7741 ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa cgcattacct
     7801 gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc gatccccatt
     7861 tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa cagtccctgg
     7921 ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa accaagtacc
     7981 atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca
     8041 acgtatttgg ttacaccgtt aaagatgcca gctccggcaa gcacgccgag cacggtaatc
     8101 aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac agccagcaac
     8161 ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac
     8221 gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg
     8281 attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt
     8341 ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag
     8401 cggaatgtaa tggaacaccg ccatgatttc cgcttctttc agaaagttaa tcaacgcgct
     8461 ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc
     8521 gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg catcgtagta
     8581 gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca gttgcgccca
     8641 caggtacgca gcttgcagat cggacatcaa atagctggag ccaatatcgc gccaggtata
     8701 tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc
     8761 ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt
     8821 atagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg
     8881 ccctttgtaa gtggacatca cgccctgagc ggcatcttct accacaaaca gattatgctt
     8941 tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat ggaccggcac
     9001 gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc gtttcgtcga tgttcatggt
     9061 gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg cattggcggt
     9121 ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag
     9181 cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa
     9241 acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc
     9301 gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa
     9361 tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt
     9421 gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt
     9481 taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc
     9541 cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acataaccgc
     9601 gaataccgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg
     9661 cattttcaat ccactgtgca taaaagcgtc cgctggcgtc aggcgcatac cacggcgcac
     9721 gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct
     9781 cttgtgccac tacagcacca ctatcactga cattgttcac gggtagtgcc aaatcaactt
     9841 caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc gtatttgatg
     9901 ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac gtctggcgtc aggagcggtg
     9961 cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt tcccaggtta
    10021 gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca
    10081 gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc
    10141 caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca
    10201 aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc
    10261 ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc
    10321 tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg
    10381 tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc
    10441 gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg gtccatcacc
    10501 tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg
    10561 aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc accgttgagg
    10621 aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcgtat
    10681 tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct
    10741 tccggcgtag tgatgatgag aatttcgcgg atatcggcca acatcagcac cgacagcgga
    10801 tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc gcgcgtaatc
    10861 ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct
    10921 gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc
    10981 caccagcttt cattagccag ataccactgc accgttttac gcattccact ttcaaaggtt
    11041 tcctgtggca gccagccaag ttcacgggca attttcgaag catcaatggc ataacgcaga
    11101 tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcac cacgccgtgc
    11161 ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc
    11221 ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg
    11281 caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg ctgcccgttg
    11341 ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg gatcagtttt
    11401 tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgataagcgt cggcagaccg
    11461 taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg
    11521 ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca
    11581 tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc
    11641 gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca
    11701 ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg
    11761 acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc gcagatatca
    11821 actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg
    11881 gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg tttcgttgat gatataacgc
    11941 accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc
    12001 agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttaa
    12061 actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct
    12121 gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa
    12181 ccagagtttc gccgctatgc cactgggcga tcagttcagc gatttccatc gccgggcttt
    12241 cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac
    12301 tggcgcgttt atcggtagcc gccaggcaat cagccaccgc cgctttcacc tgatcgataa
    12361 cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctggg
    12421 ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc acgccagggc
    12481 caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt
    12541 taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc
    12601 ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg acgacacact
    12661 caccttcgag gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc ggcgtcatac
    12721 caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt
    12781 ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtgagat
    12841 ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg
    12901 attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt
    12961 caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc
    13021 aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt
    13081 ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa
    13141 ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg
    13201 ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga
    13261 tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg
    13321 gttatgggcg cggctcatag tttgatattc gttttcgtct tttaaaagac gcgtcacttc
    13381 ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc
    13441 cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc
    13501 ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg
    13561 taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac
    13621 cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt
    13681 ggtggcaatg tctgccaacg cgtggcagat ttcttcaaag ccacgaccga aactttcacg
    13741 cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt aatttgccgc
    13801 cagttctgaa cgcagcgtat cgctgctcat cacctggtca cgcacccata acagtgcatc
    13861 aataactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt
    13921 ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg
    13981 gttagcctct tccggccacg gcgaataaag atcgcccgtg cgcagaccag cctcaacgtg
    14041 accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc
    14101 gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag
    14161 aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc
    14221 aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac
    14281 gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gcgccatctt
    14341 gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata
    14401 agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta
    14461 atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac
    14521 gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa
    14581 aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat
    14641 agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg
    14701 gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac
    14761 tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ttcaatactg ttcatccggc
    14821 ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgtgctttc atctggatgg
    14881 tacgcgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg
    14941 caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttca gcaataagct
    15001 tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg ttgttaatca
    15061 tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt ttgtaatagt
    15121 cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt
    15181 tataggcttc gtccatgacc gatggttggt cggcagaagc catgtttgaa cggacatcca
    15241 ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat
    15301 cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta
    15361 acgcaaatgc cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca
    15421 acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg
    15481 tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct
    15541 gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata
    15601 gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata
    15661 ttctgccagc acgccaatgg aagcgagcag tgccgcagca agggtaatca gcacaaacgc
    15721 ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga
    15781 gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg
    15841 aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc cctgggtcgt
    15901 ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat cacccataaa
    15961 gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc
    16021 gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc
    16081 gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc catcaaccat
    16141 gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc
    16201 gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa
    16261 caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc
    16321 aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga
    16381 tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga
    16441 aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac
    16501 taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa
    16561 aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa
    16621 ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc
    16681 caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacac gccagcaaat
    16741 tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata aaagcaaaac
    16801 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
    16861 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
    16921 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
    16981 gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
    17041 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
    17101 tcgtagataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatagtc
    17161 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg
    17221 gcgttggcat ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa
    17281 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
    17341 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
    17401 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
    17461 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
    17521 tgggcaatgt tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca
    17581 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta
    17641 gaaccgttac cacgttccat acgcagacga gaatttgcgt gcagcggggt taagttctca
    17701 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
    17761 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
    17821 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
    17881 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
    17941 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
    18001 tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc
    18061 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
    18121 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
    18181 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
    18241 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
    18301 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
    18361 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
    18421 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
    18481 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
    18541 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
    18601 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
    18661 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
    18721 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
    18781 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
    18841 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
    18901 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
    18961 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
    19021 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
    19081 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc
    19141 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac
    19201 cgctggcgga agctactggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca
    19261 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
    19321 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gttctggatg
    19381 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
    19441 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
    19501 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
    19561 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
    19621 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
    19681 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
    19741 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
    19801 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
    19861 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
    19921 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
    19981 tgaatttgcc tgctattgaa acctatattg gtcactcaat tccggtaagc aaatacaatc
    20041 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca
    20101 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
    20161 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac
    20221 tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag
    20281 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
    20341 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa
    20401 gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc
    20461 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
    20521 cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt tattgccaat
    20581 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg
    20641 atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata
    20701 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
    20761 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag
    20821 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
    20881 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
    20941 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
    21001 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
    21061 gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
    21121 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
    21181 ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc
    21241 tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat
    21301 caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
    21361 cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat
    21421 ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg
    21481 ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta
    21541 ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc
    21601 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
    21661 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
    21721 gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
    21781 ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca
    21841 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca
    21901 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
    21961 gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
    22021 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
    22081 catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
    22141 gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt
    22201 cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg
    22261 tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt
    22321 tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg
    22381 attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca
    22441 gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc
    22501 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
    22561 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa
    22621 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
    22681 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
    22741 ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga taaagaatag
    22801 cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc
    22861 gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca
    22921 gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg
    22981 ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg
    23041 gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag gagtagatcg
    23101 actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
    23161 gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag cgaattttgt
    23221 tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca
    23281 gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc ccataacaat
    23341 gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga
    23401 gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt
    23461 caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg
    23521 aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg
    23581 tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac
    23641 gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct
    23701 gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga gttttaccgg
    23761 aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
    23821 cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga
    23881 atcgcatttg aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc
    23941 agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga aaatatcggg
    24001 tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtgtac tcgtggcgat
    24061 taatttcagt cctaaggttg gtgaaatact ggattgcaac tttggtaact atcctgttag
    24121 ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg aaatgataaa
    24181 aaacaggtta gtt
//
LOCUS       DAZOYS010000047        23992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOYS010000047 DAZOYS010000000
VERSION     DAZOYS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.047"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..108)
                     /locus_tag="ACHUN9_001639"
     CDS             complement(<1..108)
                     /locus_tag="ACHUN9_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0695061.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYI"
     gene            complement(166..399)
                     /locus_tag="ACHUN9_001640"
     CDS             complement(166..399)
                     /locus_tag="ACHUN9_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0695062.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            788..1333
                     /locus_tag="ACHUN9_001641"
     CDS             788..1333
                     /locus_tag="ACHUN9_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0695063.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1308..3233
                     /locus_tag="ACHUN9_001642"
     CDS             1308..3233
                     /locus_tag="ACHUN9_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0695064.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3230..3436
                     /locus_tag="ACHUN9_001643"
     CDS             3230..3436
                     /locus_tag="ACHUN9_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0695065.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3433..5034
                     /locus_tag="ACHUN9_001644"
     CDS             3433..5034
                     /locus_tag="ACHUN9_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0695066.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5015..6334
                     /locus_tag="ACHUN9_001645"
     CDS             5015..6334
                     /locus_tag="ACHUN9_001645"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0695067.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIACLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6344..6676
                     /locus_tag="ACHUN9_001646"
     CDS             6344..6676
                     /locus_tag="ACHUN9_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0695068.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6732..7757
                     /locus_tag="ACHUN9_001647"
     CDS             6732..7757
                     /locus_tag="ACHUN9_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0695069.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7799..8197
                     /locus_tag="ACHUN9_001648"
     CDS             7799..8197
                     /locus_tag="ACHUN9_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0695070.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8209..8562
                     /locus_tag="ACHUN9_001649"
     CDS             8209..8562
                     /locus_tag="ACHUN9_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0695071.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8574..9152
                     /locus_tag="ACHUN9_001650"
     CDS             8574..9152
                     /locus_tag="ACHUN9_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695072.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9149..9544
                     /locus_tag="ACHUN9_001651"
     CDS             9149..9544
                     /locus_tag="ACHUN9_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0695073.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9552..10292
                     /locus_tag="ACHUN9_001652"
     CDS             9552..10292
                     /locus_tag="ACHUN9_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695074.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10308..10730
                     /locus_tag="ACHUN9_001653"
     CDS             10308..10730
                     /locus_tag="ACHUN9_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0695075.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10712..11146
                     /locus_tag="ACHUN9_001654"
     CDS             10712..11146
                     /locus_tag="ACHUN9_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0695076.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11139..13700
                     /locus_tag="ACHUN9_001655"
     CDS             11139..13700
                     /locus_tag="ACHUN9_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0695077.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13697..14026
                     /locus_tag="ACHUN9_001656"
     CDS             13697..14026
                     /locus_tag="ACHUN9_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695078.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14026..14724
                     /locus_tag="ACHUN9_001657"
     CDS             14026..14724
                     /locus_tag="ACHUN9_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0695079.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14730..15473
                     /locus_tag="ACHUN9_001658"
     CDS             14730..15473
                     /locus_tag="ACHUN9_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0695080.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15470..16042
                     /locus_tag="ACHUN9_001659"
     CDS             15470..16042
                     /locus_tag="ACHUN9_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0695081.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16103..19600
                     /locus_tag="ACHUN9_001660"
     CDS             16103..19600
                     /locus_tag="ACHUN9_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0695082.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REVTLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19671..20187
                     /locus_tag="ACHUN9_001661"
                     /pseudo
     CDS             19671..20187
                     /locus_tag="ACHUN9_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20252..23335
                     /locus_tag="ACHUN9_001662"
     CDS             20252..23335
                     /locus_tag="ACHUN9_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0695083.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23335..23851
                     /locus_tag="ACHUN9_001663"
                     /pseudo
     CDS             23335..23851
                     /locus_tag="ACHUN9_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         5669 a         6013 c         7337 g         4973 t
ORIGIN      
        1 aatgtaatgt gagacattgt gacgttttag ttcagaataa aaccagtgat agtttaaatt
       61 atttcgcact ttatcgaata tttgtttaaa aatggcaacc tgagccattg tagtaccttc
      121 catgtgatat gagggggcgt agtctgcacg attatctaaa ttgcttcaat ctggtctaac
      181 ctgttttctg agcaattcag taatgtcact cttttctttg tttgcttcag gcgaaactct
      241 tttttctgag cacagtctcc ggcggcaggc ttcaatgacc caggctgaga aattcccgga
      301 ccctttttga acaagagcga tgttaatttg ttcaatcatt tggttaggaa agcggatgtt
      361 gcgggttgtt gttctgcggg ttctgttctt cgttgacatg aggttgccct gtattcagtg
      421 tcgctgattt gtattgtctg aagttgtttt tacgttaagt tgatgcagat caattaatac
      481 gatacctgcg tcataattga ttatttgacg tggtttgatg gcgtagatgc acgttgtgac
      541 atgtagatga taattattat cattttgcgg gtcctttccg gcgatccgac aggttacggg
      601 gcggaaggcg cgcgggtttt cgctatttat gacaattttc cggtttaagg cgtttccgtt
      661 cttcttcgcc gtaacctaat gtttttattt aaaacacccc ctgaaaagaa aggaaacgac
      721 aggtgctgaa aacgggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga
      781 atgaacaatg gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac
      841 cattcagaac tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga
      901 ggtgctttat gactctgccg ccgttataag atggtatgcc gaaagggatg ctgaaattga
      961 gaacgaaaag ctgcgccggg aagttgaaga actgcggcag gccagcgaga cagatctcca
     1021 gccagggact attgagtacg aacgccatcg acttacgcgt gcgcaggccg acgcacagga
     1081 gctgaaaaat gccagagact ccgctgaagt ggtggaaacc gcattctgta ctttcgtgct
     1141 gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg atccccctgt cggtgcagcg
     1201 gcgttttccg gaactggaaa accgacatgt tgatttcctg aaacgggata tcatcaaagc
     1261 catgaacaaa gcagccgcgc tggatgaact gataccgggg ttgctgagtg aatatatcga
     1321 acagtcaggt taacaggctg cggcattttg tccgcgccgg gcttcgctca ctgttcaggc
     1381 cggagccaca gaccgccgtt gaatgggcgg atgctaatta ctatctcccg aaagaatccg
     1441 cataccagga agggcgctgg gaaacactgc cctttcagcg ggccatcatg aatgcgatgg
     1501 gcagcgacta catccgtgag gtgaatgtgg tgaagtctgc ccgtgtcggt tattccaaaa
     1561 tgctgctggg tgtttatgcc tactttatag agcataagca gcgcaacaca cttatctggt
     1621 tgccgacgga tggtgatgcc gagaacttta tgaaaaccca cgttgagccg accatccgcg
     1681 atattccgtc gctgctggcg ctggctccgt ggtatggcaa aaagcaccgg gataacacgc
     1741 tcactatgaa gcgtttttcc aatggtcgtg gcttctggtg cctgggcggt aaagcggcaa
     1801 aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga tgaacttgct gcctttgatg
     1861 aggatattga acaggaaggc tctccgacgt tccttggcga caaacgtatt gaaggctcgg
     1921 tctggccaaa gtccatccgt ggctccacgc ccaaagtgag aggcacctgc cagattgagc
     1981 gtgcagccag tgaatccccg cattttatgc gttttcatgt tgcctgcccg cactgcgggg
     2041 aggagcagta tcttaaattt ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg
     2101 atgacccctc cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg
     2161 agctggactt tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg
     2221 gcattctctg gttttcgtca tccggtgaag agattgagcc acctgacagt gtgacctttc
     2281 acatctggac agcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga
     2341 aaacgaaagg ggatacggga aaacgtaaaa ccttcgtaaa caccacgctc ggtgagacgt
     2401 gggaggcgaa aattggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt
     2461 attcagcgcc cgttcctgac cgtgtggctt acctgaccgc cggtatcgac tcccagctgg
     2521 atcgctacga aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgatc
     2581 ggcagattat tatgggccgc cacgacgatg aacagacgct gctgcgtgtg gatgaggcca
     2641 tcaataaaac ctatacccgc cggaatggtg cagaaatgtc gatatcccgt atctgctggg
     2701 atattggcgg gattgacccg accattgtgt atgaacgctc gaaaaagcat gggctgttcc
     2761 gggtgatccc cattaaaggg gcatccgtct acggtaagcc ggtggccagc atgcctcgta
     2821 agcgaaacaa aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga
     2881 tttataaccg cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact
     2941 tcccgaataa cccggatatt tttgatctga ccgaagcgca gcagctgact gctgaagagc
     3001 aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca
     3061 atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt atttcccgct
     3121 ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt gcagcaacca
     3181 acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca
     3241 ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggta aacgggtggc
     3301 aacggtacag aaagacggac ggcgagtgga gtttacggcc acttccgtgt ctgacctgaa
     3361 aaaatacatt gcggagctgg aggtgcagac cggcatgaca cagcgacgca ggggacctgc
     3421 aggattttat gtatgaaaac gcccaccatt cccacccttc tgggaccgga cggcatgaca
     3481 tcgctgcgtg aatatgccgg ttatcacggc ggtggcagcg gatttggtgg gcagttgcgg
     3541 gcgtggaacc caccgggtga aagtgtggat gcagccctgc tgcccaactt tacccgtggc
     3601 aatgcccgcg cagacgatct ggtacgcaat aacggctatg ctgccaacgc catccagctg
     3661 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     3721 ctgggcatcg gggaggaaga cgcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     3781 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     3841 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     3901 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     3961 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     4021 gacagcggtg cggcgctggg atattacgtc agcgaggacg gctatcctgg ctggatgccg
     4081 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcctcgtt cattcacgtt
     4141 tttgaacccg tggaggacgg gcagacccgc ggtgcaaatg tgttttacag cgtaatggag
     4201 cagatgaaga tgctcgacac gctgcagaac acgcagctgc agagcgccat tgtgaaggcg
     4261 atgtatgccg ccaccattga aagtgagctg gatacgcagt cagcgatgga ttttattctg
     4321 ggcgcgaaca gtaaggagca gcgggaaagg ctgacgggct ggattggtga aattgccgcg
     4381 tattacgccg cagcaccggt ccgtctggga ggcgcaaaag tgccgcacct gatgccgggg
     4441 gactcactga acctgcagac ggctcaggac acggataacg gctactccgt gtttgagcag
     4501 tcactgttgc ggtatatcgc tgccgggctg ggtgtctcgt atgagcaact ttcccggaat
     4561 tacgcccaga tgagctactc cacggcacgg gccagtgcga acgagtcgtg ggcgtacttt
     4621 atggggcggc gaaaattcgt cgcatcccgt caggcgagcc agatgtttct gtgctggctg
     4681 gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt cagctttcag
     4741 gaagcccgca gtgcctgggg gaactgcgac tggataggct ccggtcgtat ggccatcgat
     4801 ggtctgaaag aagttcagga agcggtgatg ctgatagaag ccggactgag cacctacgag
     4861 aaagagtgcg cgaaacgcgg tgacgactat caggaaattt ttgcccagca ggtccgtgaa
     4921 acgatggagc gccgcgcagc tggtcttaaa ccgcccgcct gggcggctgc ggcatttgaa
     4981 tccgggctgc gacaatcaac agaggaggag aagagtgaca gcagagctgc gtaatctccc
     5041 gcatattgcc agcatggctt ttaatgagcc gctgatgctt gaacccgcct atgcgcgggt
     5101 tttcttttgt gcgcttgcag gccagcttgg gatcagctgc ctgacggatg cagtatccgg
     5161 cgacagcctg actgccggag aggcacccgc ggcgctggcg ttatccggtg atgatgacgg
     5221 accacgacag gcccgcagtt atcaggtaat gaacggcatc gccgtgctgc cggtgtccgg
     5281 cacgctggtc agccggacgc gggcgctgca gccgtactcg gggatgaccg gttacaacgg
     5341 cattatcgcc tgtctgcaac aggctgccag cgacccgatg gtggacggca ttctgctcga
     5401 tatggacaca ccgggcggaa tggtggcggg agcatttgac tgcgctgaca tcatcgcccg
     5461 tgtgcgtgac ataaagccgg tatgggcgct ggccaatgac atgaactgca gcgcaggtca
     5521 gctgcttgcc agtgccgcct cccggcgtct ggtcacgcag accgcccgga caggctccat
     5581 cggcgtcatg atggctcaca gtaattacgg cgctgcactg gagaaacagg gcgtggaaat
     5641 cacgctgatt tacagcggca gccataaggt ggatggcaac ccctacagcc atctaccgga
     5701 tgatgtccgg gaaacattgc agtcccggat ggatgcaacc cgccggatgt ttgcacagaa
     5761 ggtgtcggca tataccggcc tgtccgtgca ggctgtgctg gataccgagg ctgcagtgta
     5821 cagcggtcag gaggccattg atgccggact ggctgatgaa cttgttaaca gtaccgatgc
     5881 gatcaccgtc atgcgtgatg cactggatgc acgtaaatcc cgtctctcag gagggcgaat
     5941 gaccaaagag actcaatcaa caactgtttc agccactgct tcgcaggctg acgttactgg
     6001 cgtggtgcaa gcgacggagg gcgagaacgc cagcgcggcg cagccggacg tgaacgcgca
     6061 gatcaccgca gcggttgcgg cagaaaacag tcgcattatg gggatcctca actgtgagga
     6121 ggctcacgga cgcgaagaac aggcacgcgt gctggccgaa acccccggta tgaccgtgga
     6181 aacggcccgc cgtattctgg ccgcagcacc acagagtgca cagacgcgca gtgacactgc
     6241 gctggatcgt ctgatgcagg gggcaccggc accgctggct gcaggtaacc cggcatctga
     6301 tgccgttaac gatttgctga acacaccagt gtaagggatg tttatgacga gcaaagaaac
     6361 ctttacccat taccagccgc tgggcaatag tgacccggct cataccgcaa ccgcgcccgg
     6421 cggattgagt gcgaaagcgc ctgcaatgac cccgctgatg ctggacacct ccacccgtaa
     6481 gctggttgcg tgggatggca ccaccgacgg tgctgccgtt ggcattcttg cggttgctgc
     6541 tgaccagacc agcaccacac tgacgttcta caagtccggc acgttccgtt atgaggatgt
     6601 gctctggccg gaggctgcca gcgacgagac gaaaaaacgg accgtgtttg ccggaacggc
     6661 aatcagcatc gtttaacttt acccttcatc actaaaggcc gcctgtgcgg ctttttttac
     6721 gggatttttt tatgtcgatg tacacaaccg cccagctgct ggcggcaaat gagcagaaat
     6781 ttaagtttga tccgctgttt ctgcgtctct ttttccgtga gagctatccc ttcactacgg
     6841 agaaagtcta tctctcacaa attccgggac tggtaaacat ggcgctgtac gtttcgccga
     6901 ttgtttccgg tgaggttatc cgttcccgtg gcggctccac ctctgaattt acgccgggat
     6961 atgtcaaacc caagcatgag gtgaatccgc agatgaccct gcgtcgcctg ccggatgaag
     7021 atccgcagaa tctggcggac ccggcttacc gccgccgtcg catcatcatg cagaacatgc
     7081 gtgacgaaga gctggccatt gctcaggtcg aagagatgca ggcagtttct gccgtgctca
     7141 agggcaaata caccatgacc ggtgaagcct tcgatccggt tgaggtggat atgggccgca
     7201 gtgcggcgaa caacatcaca cagtccggcg tcacggagtg gagcaagcgt gacaagtcca
     7261 cgtatgaccc gaccgacgat atcgaagcct acgcgctgaa cgccagcggt gtggtgaata
     7321 tcatcgtgtt tgacccgaaa ggctgggcgc tgttccgttc cttcaaagcc gtcaaggaga
     7381 agctggatac ccgtcgcggc tctcattccg agctggagac agcggtaaaa gacctgggca
     7441 aagcggtgtc ctataaggga atgtatggcg atgtggccat cgtcgtgtat tccggacagt
     7501 acgtggaaaa cggcgtcaaa aagaacttcc tgccggacaa cacgatggtg ctggggaaca
     7561 ctcaggctcg cggtctgcgc acctatggct gcattcagga tgcggacgca cagcgcgaag
     7621 gcattaacgc ctctgcccgt tacccgaaaa actgggtgac caccggcgat ccggcgcgtg
     7681 agttcaccat gattcagtca gcaccgctga tgctgctggc tgatcctgat gagttcgtgt
     7741 ccgttcaact ggcgtaatca tggcccttcg gggccatttt ctctctgtgg aggagttcat
     7801 gacgaaagat gaactgattg cccgtcttca ggagctgggt gagcaattga accgcgatgt
     7861 cagtttgacg ggaacgaaag aagaactggc gcttcgtgtg gcagagctgg aagaagagct
     7921 tgatgacacg gatgaaactg ccggtcagga cacccctctc agccgggaaa atgtgctgac
     7981 cgggcatgaa aatgaggtgg tatcagcgca gccggatacc gtgattcagg atacggctga
     8041 actggtcacg gtcgtggcac tggtgacgct gcatactgat gcacttcacg ccacgcggga
     8101 taaacctgtg gcatttgtgc tgccgggaac ggcgtttcgt gtctctgccg gtgtggcagc
     8161 cgaaatgaca gagcgcggcc tggccagaat gcaataacgg gaggcgctgt ggctgatttc
     8221 gataacctgt tcgatgctgc cattgcccgc gccgatgaaa cgatacgcgg gtacatggga
     8281 acgtcagcca ccatgacatc cggtgagcag tcaggtgcgg tgatacgtgg tgtttttgat
     8341 gaccctgaaa atatcagcta tgccggacag ggcgtgcgcg ttgaaggctc cagcccgtcc
     8401 ctgtttgtcc ggactgatga ggtgcggcag ctgcggcgtg gagacacgct gaccatcggt
     8461 gaggaaaatt tctgggtaga tcgggtttcg ccggatgatg gcggaagttg tcatctctgg
     8521 cttggacggg gcgtaccgcc tgccgttaac cgtcgccgct gaaaggggga tgtatggcca
     8581 taaaaggtct tgagcaggcc gttgaaaacc tcagccgtat cagcaaaacg gcggtgcccg
     8641 gtgccgccgc aatggccatt aaccgcgttg cttcatccgc gatatcgcag tctgcgtcac
     8701 aggttgcccg tgagacaaag gtacgccgga aactggtaaa ggaaagggcc aggctgaaaa
     8761 gggccacggt caaaaatccg caggccagaa tcaaagttaa ccggggggat ttgcccgtaa
     8821 tcaagctggg taatgcgcgg gttgtcctgt cccgccgcag gcgtcataaa aaggggcagc
     8881 gttcatccct gaaaggtggc ggcagcgtgc ttgtggtggg taaccgtcgt attcccggcg
     8941 cgtttattca gcaactgaaa aacggccggt ggcatgttat gcagcgtgtg gccgggaaaa
     9001 accgttaccc cattgatgtg gtgaaaatcc cgatggcggt gccgctgacc acggcgttta
     9061 aacagaatat tgaacggata cggcgtgaac gtcttccgaa agagctgggc tatgcgctgc
     9121 agcatcaact gagaatggta ataaagcgat gaaacatact gaactccgtg cagccgtact
     9181 ggatgcactg gagaagcatg acaccggggc gacgcttttt gatggtcgcc ccgctgtttt
     9241 tgatgaggaa gattttccgg caattgccgt ttatctcacc ggcgctgaat acacgggcga
     9301 agagctggac agcgatacct ggcaggcgga gctgcatatt gaagttttcc tgcctgctca
     9361 ggtgccggat tcagagctgg attcgtggat ggagtcccgg atttatccgg tgatgagcga
     9421 tatcccggca ctgtcagatt tgatcaccag tatggtggcc agcggctatg actaccggcg
     9481 cgacgatgat gcgggcctgt ggagttcagc cgatctgact tatgtcatta tctatgaaat
     9541 gtgaggacgc tatgcctgta ccaaatccta caatgccggt gaaaggtgcc gggaccaccc
     9601 tgtgggttta taaggggagc ggtgaccctt atgcgaaccc gctttcagac gttgactggt
     9661 cgcgtctggc aaaagttaaa gacctgacgc ccggcgaact gaccgctgag tcctatgacg
     9721 acagctatct cgatgatgaa gatgcagact ggactgcgac cgggcagggg cagaaatctg
     9781 ccggagatac cagcttcacg ctggcgtgga tgcccggaga gcaggggcag caggcgctgc
     9841 tggcgtggtt taatgaaggt gatacccgtg cctataaaat ccgcttcccg aacggcacgg
     9901 tcgatgtgtt ccgtggctgg gtcagcagta tcggtaaggc ggtgacggcg aaggaggtga
     9961 ttacccgcac ggtgaaggtc accaatgtgg gacgtccgtc gatggcagaa gatcgcagca
    10021 cggtgacggc gacgaccggc atgacggtga cacccgccag tgcttccgta gtgaaagggc
    10081 agagcaccac gctgaccgtg gcattccagc cggatggcgc aaccgacaag agcttccgtg
    10141 cggtgtctgc ggataaaaca aaagccaccg tgtcggtcag tggtatgacc atcaccgtga
    10201 aaggtgttgc tgcaggcaag gtcaacattc cggtcgtatc tggtaatggt gagtttgctg
    10261 cggttgcaga aatcaacgtc accgccagtt aatccggaga gtcagcgatg ttcctgaaaa
    10321 ccgaatcatt tgaacataac ggtgtgaccg tcacgctttc tgaactgtca gccctgcagc
    10381 gtattgagca tctcgacctg atgaaacggc aggcagaaca ggcggagtca gacagcaacc
    10441 ggaagtttac tgtggaagac gccatcagaa ccggcgcttt tgtggttgcg atgtccctgt
    10501 ggcataacca tccgaagaag acgcagatgc cgtccatgaa tgaagccgtt aaacagattg
    10561 agcaggaagt gcttaccacc tggcccacag aggcaatttc tcatgctgaa aacgtggtgt
    10621 accggctgtc cggtatgtat gagtttgtgg tgaatgatgc tcctgaacag gcagaggacg
    10681 ccgggcctgc agagcctgtt tctgcgggaa agtgttcgac ggtgagctga gttttgccct
    10741 gaaactggcg cgtgagatgg ggcgacccga ctggcgcgcc atgcttgccg ggatgtcatc
    10801 cacggagtat gccgactggc accgctttta cagtacccat tattttcatg atgttctgct
    10861 ggatatgcac ttttccgggc tgacgtacac cgtgctcagc ctgtttttca gcgatccgga
    10921 gatgcatccg ctggatttca gtctgctgaa ccggcgcgag gctgacgaag agcctgaaga
    10981 tgatgtgctg atgcagaaag cggcagggct tgccggaggt gtccgctttg gcccggacgg
    11041 gaatgaagtt atccccgctt ccccggatgt ggcggacatg acggaggatg acgtaatgct
    11101 gatgacagta tcagaaggga tcgcaggagg agtccggtat ggctgaaccg gtaggcgatc
    11161 tggtcgttga tttgagtctg gatgcggcca gatttgacga gcagatggcc agagtcaggc
    11221 gtcatttttc cggtacggaa agtgatgcga aaaaaacagc ggcagtcgtt gaacagtcgc
    11281 tgagccgaca ggcgctggct gcacagaaag cggggatttc cgtcgggcag tataaagccg
    11341 ccatgcgtat gctgcctgca cagttcaccg acgtggccac gcagcttgca ggggggcaga
    11401 atccctggct gatcctgctg caacagggtg gtcaggttaa ggactccttc ggcgggatga
    11461 tccccatgtt cagggggctt gccggcgcga tcaccctgcc gatggtgggg gccacctcgc
    11521 tggcggtggc gaccggtgcg ctggcgtatg cctggtatca gggcaactca accctgtccg
    11581 atttcaacaa aacgctggtc ctttccggta atcaggcggg actgacggca gatcgtatgc
    11641 tggtcctgtc cagagccggg caggcggcag ggctgacgtt taaccagacc agcgagtcac
    11701 tcagcgcact ggttaaggcg ggggtaagcg gtgaggctca gattgcgtcc atcagccaga
    11761 gtgtggcgcg tttctcctct gcatccggcg tggaggtgga caaggtcgct gaagccttcg
    11821 ggaagctgac cacagacccg acgtcggggc tgacggcgat ggcacgccag ttccataacg
    11881 tgacggcgga gcagattgcg tatgttgctc agttgcagcg ttccggcgat gaagccgggg
    11941 cattgcaggc ggcgaacgag gccgcaacga aagggtttga tgaccagacc cgccgcctga
    12001 aagagaacat gggcacgctg gagacctggg cagacaggac tgcacgtgca ttcaaatcca
    12061 tgtgggatgc ggtgctggat attggtcgtc ctgataccgc gcaggagatg ctgattaagg
    12121 cagaggctgc gtttaagaaa gcagacgaca tctggaatct gcgcaaggat gattatttcg
    12181 ttaacgatga agcacgggcg cgttactggg atgatcgtga aaaaaaacgc ctggagcggg
    12241 atgcagccca aaagagggta gatcaacagc gtcaacagga caaaaatgcg cagcagcaga
    12301 gcgataccga agcgtcacgg ctgaaatata ccgaagaggc gcagaaggct tacgaacggc
    12361 tgcagacgcc gctggagaaa tataccgccc gtcaggaaga actgaacaag gcactgaaag
    12421 acggaaaaat cctgcaggca gattacaaca cgctgatggc ggcggcgaaa aaggattatg
    12481 aagcgacgct gaaaaagccg aaacagtccg gcgtgaaggt gtatgcgggc gatcgtcagg
    12541 aagacagtgc tcatgcggcc ctgctgacgc ttcaggcaga actccggacg ctggagaagc
    12601 atgccggagc gaatgagaaa atcagccagc agcgccggga tttgtggaag gcagaaagtc
    12661 agttcgcggt actggaggag gcggcacaac gtcgccagct gtccgcacag gagaaatccc
    12721 tgctggcgca taaagacgag acgctggagt acaaacgcca gctggctgca cttggtgaca
    12781 aggttacgta tcaggagcac ctgaatgcgc tggcgcagca ggcggataaa ttcgcacagc
    12841 agcaacgggc aaaacgggca gccattgatg cgaaaaaccg ggggctgact gaccggcagg
    12901 cagcgcggga agccacggaa cagcgcctga aggaacagta tggcgataat cctctggcgc
    12961 tgaataacgt catgtcagag cagaaaaaga cctgggcggc tgaagaccag cttcgcggga
    13021 gctggatggc aggcctgaag tccggctgga gtgagtggga agagagcgcc acggacagta
    13081 tgtcacaggt taaaagtgca gccacgcaga cctttgatgg tattgcacag aatatggcgg
    13141 cgatgctgac cggcagtgaa cagaactggc gcagcttcac ccgctccgtg ctgtccatga
    13201 tgacagaaat tctgcttaag caggcaatgg tggggattgt cgggagtatc ggcagcgcca
    13261 ttggcggggc tgttggtggc ggcgcatccg cgtcaggtgg tacagccatt caggccgctg
    13321 cggcgaaatt ccattttgca accgggggat ttacgggaac cggcggcaaa tatgagccag
    13381 cggggattgt tcaccgtggt gaatttgtct tcacgaagga ggcaaccagc cggattggcg
    13441 tggggaatct ctaccggctg atgcgcggct atgcggaagg tggttatgtg ggcggtgccg
    13501 gaagtccggc gcagatgcgg cgggctgaag gcattaattt taatcagaac aatcacgtgg
    13561 tgattcagaa cgacggtacg aatggtctgc caggtccaca gatgatgaag gccgtgtatg
    13621 acatggcccg caagggtgcc cgtgatgaaa ttcagacaca gatgcgtgat ggtggcctgt
    13681 tctccggagg tggacgatga aaaccttccg ctggaaagtg aaacccggta tggatgtggc
    13741 ttcggcccct tctgtaagaa aggtgcgctt tggtgatggc tattcccagc gagcgcctgc
    13801 cgggctgaat gccaacctga aaacgtacag cgtgacgctt tctgtccccc gtgaggaggc
    13861 catggtactg gagtcgtttc tggaagagca cgggggctgg aaagcctttc tgtggacgcc
    13921 gccttatgag tggcggcaga taaaggtgac ctgcgcaaaa tggtcgtcgc gggtcagtat
    13981 gctgcgtgtt gagttcagcg cagagtttga acaggtggtg aactgatgca ggatatccgg
    14041 caggaaacac tgaatgaatg cacccgtgcg gagcagtcgg ccagcgtggt gctctgggaa
    14101 atcgatctga cagaggtcgg tggagaacgt tattttttct gtaatgagca gaacgaaaaa
    14161 ggtgagccgg tcacctggca ggggcgacag tatcagccgt atcccattca ggggagtggt
    14221 tttgaactga atggcaaagg caccagtacg cgccccacgc tgatggtttc taacctgtac
    14281 ggtatggtca ccgggatggc ggaagatcta cagagtctgg tcggcggaac ggtggtccgg
    14341 cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg tcaacggaaa cagtgacgcc
    14401 gatccggagc aggaggtgat cagccgctgg cgcattgagc agtgcagcga actgagcgcg
    14461 gtgagtgcct cttttgtact gtccacgccg acggaaacag atggcgctgt ttttccggga
    14521 cgtatcatgc tggccaacac ctgcacctgg acctatcgcg gcgatgagtg cggttatcac
    14581 ggtccggcgg tcgcggatga atatgaccag ccaacgtccg acatcacgaa ggataaatgc
    14641 agcaaatgcc tgagtggctg taagtttcgc aataacgtcg gcaactttgg cggcttcctt
    14701 tccattaaca aactttcgca gtaaatccca tgacagagac agaatcagcg attctggcgc
    14761 acgcccggcg atgtgctcca gcggagtcgt gcggcttcgt ggtaagaacg ccggaggggg
    14821 aaagatattt cccctgcgtg aatatctccg gtgagccgga ggcgtatttc cgtatgtcgc
    14881 cggaagactg gctgcaggca gagatgcagg gtgagattgt ggcactggtc cacagccacc
    14941 ccggtggtct gccctggctg agtgaggccg accggcggct gcaggtgcag agtgatttgc
    15001 cgtggtggct ggtctgccgg ggggcgattc acaagttccg ctgtgtgcca catcttaccg
    15061 ggcggcgctt tgagcacggg gtgacggact gttacacgct gttccgggat gcttaccatc
    15121 tggcggggat tgagatgccg gattttcatc gtgaggatga ctggtggcgt cacggtcaga
    15181 atctctatct ggataatctg gaggccacag ggctgtatca ggtgccgttg tcagcggcgc
    15241 agccgggcga tgtgctgctg tgctgttttg gttcatcggt gccgaatcat gccgccattt
    15301 actgtggtga cggcgagctg ctgcaccata ttcctgaaca actgagcaaa cgagagaggt
    15361 ataccgacaa atggcagcga cgcacacact ccctctggcg tcaccgggca tggcacgcat
    15421 ctgcctttac ggggatttgc aacgatttgg ccgccgcatc gaccttcgtg tgaaaacggg
    15481 tgccgaagcc atccgggcga tggccacaca gctccctgcg tttcgtcaga agctgagcga
    15541 cggctggtat caggtgcgta ttgccgggca ggatgttagc acgtccggat taacggcgca
    15601 gttacatgag gctctgcctg acggcgctgt gattcatatt gttcccagag tcgccggggc
    15661 caagtcaggt ggtgtattcc agattgtcct gggagcagcc gccattgccg gatcattctt
    15721 taccgccgga gccacccttg cagcatgggg ggctgccatt ggtgccggtg gtatgaccgg
    15781 cattctgttt tctctcggtg ccagtatggt gctcggcggt gtggctcaga tgctggcacc
    15841 gaaagccaga actccccgca cacagacaac ggataacggt aagcagaaca tctatttctc
    15901 ctcactggat aacatggttg cccagggcaa tgttatgcct gttctgtatg gtgaaatgcg
    15961 cgtggggtca cgtgtggtat ctcaggagat cagcacggca gacgaagggg acggtggtca
    16021 ggttgtggtg attggtcgct gatgcaaaat gttttatgtg aaatcgcctc cgggcggttt
    16081 tatcgtttat ggagcatgac gaatgggtaa aggcagcagt aaggggcata ccccgcgcga
    16141 agcgaaggac aacctgaagt ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg
    16201 gccggttgaa ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct
    16261 ggacagtgag gggaatacca acatctccgg cgtcacggtg gtgttccggg caggtgagca
    16321 ggagcagtca ccgccggagg gatttgaatc ctccggctcc gagacggtgc tgggtacgga
    16381 agtgaaatat gacacgccga tcacccggac catcacgtcg gcaaacattg accgactgcg
    16441 ttttaccttc ggtgtacagg cactggtgga aaccacctca aagggagaca ggaatccgtc
    16501 tgaagttcgc ctgctggttc agatacagcg taacggtggc tgggtgacgg aaaaagacat
    16561 caccattaag ggcaaaacca cctcgcagta tctggcctcg gtggtggtgg ataacctgcc
    16621 gccgcgcccg tttaatatcc ggatgcgcag aatgacgccg gacagcacca cagaccagct
    16681 gcagaacaaa acgctctggt cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc
    16741 gaacacggca ctggtcggcg tacaggtgga ttcggagcag ttcggcagcc agcaggtgag
    16801 ccgtaattat catcttcgcg ggcgcattct gcaggtgccg tcgaactata atccgcagac
    16861 gcggcaatac agcggtatct gggacggaac gttaaaaccg gcatacagca acaacatggc
    16921 ctggtgtctg tgggatatgc tgacccatcc gcgctatggc atggggaaac gtcttggtgc
    16981 ggcagatgtg gataaatggg cgctgtatgt catcggccag tactgtgacc agtcggtgcc
    17041 ggacggcttt ggcagcacgg agccgcgcat cacctgtaat gcctggctga ccacacagcg
    17101 caaggcgtgg gatgtgctca gtgatttctg ctcggcgatg cgctgtatgc cggtatggaa
    17161 cgggcagacg ctgacgttcg tgcaggaccg acagtcggat aaggtgtggg cctataaccg
    17221 cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagtttca gcgccctgaa
    17281 agaccgccat aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc
    17341 gacagagctt gtggaggata cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat
    17401 ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa
    17461 aacggaactg ctggagacgc agaccgtgga tttcagcgtg ggtgctgaag ggcttcgtca
    17521 tgtaccgggc gatgtcattg aaatctgcga tgatgactat gccggtatcc gcaccggcgg
    17581 gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aagtcacgct
    17641 gccatcctcc ggcaccacgc tgataagcct ggttgacggg caggggaatc cggtcagcgt
    17701 ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg agccgtgttc ctgacggtgt
    17761 tgctgaatac agcgtgtggg ggctgaagct gccgacgctg caccagcgat tgttccgctg
    17821 cgtgagtatc cgtgagaacg acgacggcac gtatgccatc accgccgtgc agcatgtgcc
    17881 ggaaaaagaa gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt
    17941 gaatggtgtc acgccgccag cggtgcagca cctgactgcc gaagtcaccg cagacagcgg
    18001 ggaatatcag gtgctggcgc gctgggacac gccgaaggtg gtgaagggcg tgagcttcct
    18061 gctccgtctg accgtaacag cggatgacgg cagtgagcgg ctggtcagca cggcccggac
    18121 gacggaaacc acttaccgct tcacacaact ggcgctgggg aactacaggc tgacagtccg
    18181 ggcagtaaat gcgtgggggc agcagggcga tccggcgtcg gtatcgttcc ggattgccgc
    18241 accggcagcg ctgtcgcgga ttgagctgac gccgggctat tttcagataa ccgccacgcc
    18301 gcatcttgcg gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaaagcggat
    18361 tgcggatatc aggcaggttg aaaccacagc acgctatctt ggcacggcgc tgtactggat
    18421 agccgccagt atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa
    18481 caccgttggc aaatcggcat tcgtggaggc tgttggtcag ccgagtgatg atgcatccgg
    18541 ttatctggat tttttcaaag gcgagatagg gaaaacccat ctggctcagg agctgtggac
    18601 gcagattgat aacggtcagc ttgcgcctga cctggctgaa atcaggacgt ccattacgga
    18661 tgtcagcaat gaaatcacgc agaccgtcaa taagaaactg gaagaccaga gtgcggcaat
    18721 tcagcagata cagaaggttc aggttgatac aaataataac ctgaacagca tgtgggctgt
    18781 gaagctgcag cagatgcagg acggacgcct ttatatcgcg ggtattggtg ccggtattga
    18841 gaacacccct gacggcatgc agagtcaggt gctgctggcg gcggacagga ttgcgatggt
    18901 taatcctgcg aatggcaaca caaaaccgat gtttgttggt cagggcgatc agatattcat
    18961 gaacgacgtg ttcctgaaac gcctgacggc ccccaccatt accagcggtg gaaatccatc
    19021 ggcattttcc ctgactccgg acggaaagct gaccgctaaa aatgcggata tcagtggcag
    19081 tgtgaatgcg aacgccggga cgctcaacaa tgtcacggta aatgaaaact gtacgattaa
    19141 gggcatgctg gaggcgactc aggtcagagg tgacttcgtt aaagctgtat ccaaatcatt
    19201 tccgaaacag gctggtacgt ggggtaacac ggaaacacca aacgggacgg ttacagtcac
    19261 cataagcgat gatcataact ttgaccgcca gattattatt ccgcccatta tttttaacgg
    19321 tatagcgtat gacgatccgg ggagcggaaa taacccagga ggcacgcgat acacgggtta
    19381 tggttttgaa gttcgcaaaa acggcgtatt aatcgcatcc agagaaacta aaggtgccat
    19441 tcccggtagt tacagtgcag ttattgatat gcctagtggt ggtggtagcg tcactctgga
    19501 gtttaagatt ttccagaaag gcaatcaggg ggcaggcaat atcaccgact gtacggtgat
    19561 tgtgaccaaa aaagcggctt ccggcatcag tattcgttga aatatttata accccaataa
    19621 atggcgtcag gaatgacgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaag
    19681 tttgtgcagc cattttgtcc gcagccattt gcctggccgt atccggtgcg cctgcatggg
    19741 cgtctgaaca tcagtccacg ctgagcgcgg ggtatcttca tgcccggacc aacgttcccg
    19801 gcagtgatga tctgaacggg attaacgtga aataccgtta tgagtttacg gatacgctgg
    19861 ggctggtgac gtcattcagc tatgcaggag acaagaatcg ccagctgacc cgttacagcg
    19921 atacccgctg gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt
    19981 ctgtgcgcgt gaatgaatgg ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg
    20041 tgtcgacttt ttccggggat tatatccgcg taactgacaa taaggggaaa acgcacgatg
    20101 tgctgaccgg aagtgatgac ggtcgccaca gcaacacgtc tctggcgcac tgacggtttc
    20161 atcgtgggtg tcggttataa attctgatta gccaggtaac acagtgttat gacagcccgc
    20221 cggttcaggc gggctttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
    20281 agacggcaca ggaaaaccgg tacagaactg cacaatccag ctgaaagcaa aacgtaacag
    20341 caccacggtg gtggtgaaca cgctggcctc agaaaatccg gatgaagccg ggcgttacag
    20401 catggacgtt gagtacggtc agtacagcgt tattctgttg gtggaaggat tcccgccgtc
    20461 acatgccggg accatcaccg tgtatgaaga ttcccgaccc ggtacgctga atgattttct
    20521 cggtgccatg acggaggatg atgcccgtcc ggaggcactg cgccgttttg agctgatggt
    20581 ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac acggcagccg cgaagaaatc
    20641 agccagcgat gccagcacat cagcccgtga ggcggcaacc catgcgactg atgctgcaga
    20701 ttcagcacgt gcagccagca cgtcagccgg acaggccgct tcgtcggctc agtcagcgtc
    20761 ttccagcgca ggaacggcat caacaaaggc cactgaagcc tcaaaaagtg ctgccgctgc
    20821 agagtcctca aaaagcgcgg cggctaccag tgccggtgcg gcgaaaacgt tagaaacgaa
    20881 tgcggcagcg tcacaacaat cagcagccac ttctgcatcc accgcgacca cgaaagcgtc
    20941 agaagctgcc acctcagccc gggatgcgtc ggcttcaaaa gaggcggcaa aatcatcaga
    21001 aacgaacgca tcatcaagcg ccagtagcgc agcttcctcg gcgacggcgg ctgcaaattc
    21061 tgcgaaggcg gccaaaacgt ccgagacgaa cgccaggtct tctgaaacgg cagcgggaca
    21121 gagtgcctca gctgcggcag gctcaaaaac agcggctgcg tcgtctgcca gcgccgcgtc
    21181 aacaagtgcc gggcaggcct cagccagtgc caccgccgcc ggaaaatcgg cagaaagcgc
    21241 cgcatcatcc gcttcaacag ccacaacgaa ggctggcgaa gccactgagc aggccacggc
    21301 agcagcgagg tcagcttccg cagcgaagac atccgaaacg aacgcgaaag cgtcggaaac
    21361 ccgtgcagaa tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg cgtcatcggc
    21421 atcatcggcg tctgcttcaa aagatgaggc gaccagacag gcgtcagcag cgaagggcag
    21481 cgccacgacg gcatccacga aggcgacaga ggctgctggt agtgcgacgg cagcagctca
    21541 gagcaaaagt acggcggaat ctgcagcaac gcgcgctgag acagcggcaa aacgggcaga
    21601 ggatattgca tccgccgtgg cgcttgagga tgcgagcacg acgaaaaagg ggatagtaca
    21661 gctcagtagt gcgactaaca gcacttccga gtcactggcg gcaacaccaa aagccgttaa
    21721 ggccgcgtat gacctggcta acgggaaata caccgcacag gatgcaacga cagcacagaa
    21781 agggataatc cagctaagca gcgcgaccaa cagcacgtct gaaacgctgg cggcaacgcc
    21841 aaaggcagta aaagcagcca atgacaatgc tgagaaacgt ctgcagaaag atcagaacgg
    21901 tgcggatatc cctggcaaag acacctttac gaaaaatatt ggtgcctgcc gtgccttcgg
    21961 tgggtcagta agcacaacaa caggaaactg gacgactgca cagtttatcg agtggctgga
    22021 ttctcaggga gcatttaacc atccatactg gatgtgcaag ggttcctggt cttatggcaa
    22081 taataaaatc attactgata ctggctgcgg taatattcat ctcgccggag ctgtcattga
    22141 agtaatgggg ataaagtcag cgatgacgat ccgcattacc actccgacca catccagcgg
    22201 tggcggaaca accaacgccc agtttaccta tattaatcac ggaacagatt attcacctgg
    22261 ctggcgaagg gactataact ccagaaataa gccaacggca tcagagatcg gggcgctacc
    22321 gtcaggtgga acagcagtat catcagttaa tctggcttca aaaggtcggg tagccgcgct
    22381 gacagacaat acgcaggggg cagcaggtct tgagttatac gaggtgtata acaacggata
    22441 tccaacagcg tatggaaata tcattcacct gaaagggatg acagccgttg gcgaaggtga
    22501 gttactcatc ggctggagtg gtacaagcgg tgctcatgct ccggcattta ttcgttcacg
    22561 acgggatacg accgacgcaa actggtcgcc gtgggcgcag ctttacacct cggctcatcc
    22621 tcctgcagag ttttatccag tcggtgcacc aatcccgtgg ccatcagata ccgttccgtc
    22681 tggttatgcc ctgatgcagg ggcagacttt tgacaaatct gcttacccga aacttgcagc
    22741 cgcttatccg tcaggcgtga tccctgatat gcgtggctgg acgattaagg gcaagcccgc
    22801 cagtggtcgt gccgtattgt ctcaggaaca ggatggcatt aaatcgcaca cccacagcgc
    22861 cagcgcatcc agtacggatt tggggacgaa aaccacatcg tcgtttgatt acggcactaa
    22921 atccacgaat aacaccgggg cgcatacgca cagtctgagc ggctctacaa gttcagctgg
    22981 ggcccaccaa catagtcaga ctgggcccag aacgaatagc gggagtcaac ctactggaat
    23041 gtttccggca gggagtactc aagttagcgg gactaatcag gttggtatca gtggctcatt
    23101 aaccagtggc acctcccaat gggtaggtaa atcatcttca gaaggaaacc atacacactc
    23161 actgtccggc acagcagcat ctgcgggtgc acacgcgcat actgtcggta ttggtgctca
    23221 tacgcactct gttgcgattg gttcacatgg acacaccatc accgttaacg ctgctggtaa
    23281 cgcggaaaac accgtcaaaa acatcgcatt taactatatt gtgaggctcg cataatggca
    23341 ttcagaatga gtgaacaagc acggaccata aaaatttata acctgctggc cggaactaat
    23401 gaatttattg gtgaaggtga cgcatatatt ccgcctcata caggtctgcc tgcaaacatc
    23461 gtggcatctc gttgaagatc atcggggtaa aacggtttat gacgtggctt ccggcgacgc
    23521 gttatttatt tctgaactcg gctcattacc ggaaaatgtc acctggttat ccccggaagg
    23581 ggagtttcag aagtggaacg gcacagcctg ggtgaaggat acggaagcag aaaaactgtt
    23641 ccggatccgg gaggcggaag aaacaaaaaa cagcctgatg caggtagcca gtgagcatat
    23701 agcgccgctt caggatgctg tggatctgga gatcgcaacg gagaaagaaa ccttgttgct
    23761 ggaagcctgg aaaaagtatc gggtgttgct gaaccgtgtt gatacatcaa ctgcaccgga
    23821 tattgaatgg ccagtagcac ctatagggta aattcgtaat gattacctaa atacgttatt
    23881 cttttgttaa aaaagtgatt ttgctatagg tagggagtat gttcttggat taagaataaa
    23941 aaatcgccat gaaagagctt catggcgagt acataaacgt acaaaatgtg at
//
LOCUS       DAZOYS010000048        23768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOYS010000048 DAZOYS010000000
VERSION     DAZOYS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.048"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(23..3904)
                     /gene="entF"
                     /locus_tag="ACHUN9_001664"
     CDS             complement(23..3904)
                     /gene="entF"
                     /locus_tag="ACHUN9_001664"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0695084.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3901..4119)
                     /gene="ybdZ"
                     /locus_tag="ACHUN9_001665"
     CDS             complement(3901..4119)
                     /gene="ybdZ"
                     /locus_tag="ACHUN9_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0695085.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4122..5324)
                     /gene="fes"
                     /locus_tag="ACHUN9_001666"
     CDS             complement(4122..5324)
                     /gene="fes"
                     /locus_tag="ACHUN9_001666"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0695086.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5567..7807
                     /gene="fepA"
                     /locus_tag="ACHUN9_001667"
     CDS             5567..7807
                     /gene="fepA"
                     /locus_tag="ACHUN9_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0695087.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7982..8602
                     /gene="entD"
                     /locus_tag="ACHUN9_001668"
     CDS             7982..8602
                     /gene="entD"
                     /locus_tag="ACHUN9_001668"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0695088.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8724..8876)
                     /gene="hokE"
                     /locus_tag="ACHUN9_001669"
     CDS             complement(8724..8876)
                     /gene="hokE"
                     /locus_tag="ACHUN9_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0695089.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9227..9379)
                     /gene="hokE"
                     /locus_tag="ACHUN9_001670"
     CDS             complement(9227..9379)
                     /gene="hokE"
                     /locus_tag="ACHUN9_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0695090.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9348..9616
                     /locus_tag="ACHUN9_001671"
                     /pseudo
     CDS             9348..9616
                     /locus_tag="ACHUN9_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9819..10937
                     /locus_tag="ACHUN9_001672"
     CDS             9819..10937
                     /locus_tag="ACHUN9_001672"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0695091.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            11003..11251
                     /locus_tag="ACHUN9_001673"
                     /pseudo
     CDS             11003..11251
                     /locus_tag="ACHUN9_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11316..11684
                     /locus_tag="ACHUN9_001674"
     CDS             11316..11684
                     /locus_tag="ACHUN9_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0695092.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11778..12431
                     /gene="nfsB"
                     /locus_tag="ACHUN9_001675"
     CDS             11778..12431
                     /gene="nfsB"
                     /locus_tag="ACHUN9_001675"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0695093.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12539..13786
                     /gene="ybdG"
                     /locus_tag="ACHUN9_001676"
     CDS             12539..13786
                     /gene="ybdG"
                     /locus_tag="ACHUN9_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0695094.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13854..15230)
                     /gene="pheP"
                     /locus_tag="ACHUN9_001677"
     CDS             complement(13854..15230)
                     /gene="pheP"
                     /locus_tag="ACHUN9_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0695095.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15332..18475)
                     /gene="cusA"
                     /locus_tag="ACHUN9_001678"
     CDS             complement(15332..18475)
                     /gene="cusA"
                     /locus_tag="ACHUN9_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0695096.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18487..19710)
                     /gene="cusB"
                     /locus_tag="ACHUN9_001679"
     CDS             complement(18487..19710)
                     /gene="cusB"
                     /locus_tag="ACHUN9_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0695097.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19726..20058)
                     /gene="cusF"
                     /locus_tag="ACHUN9_001680"
     CDS             complement(19726..20058)
                     /gene="cusF"
                     /locus_tag="ACHUN9_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0695098.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20082..21464)
                     /gene="cusC"
                     /locus_tag="ACHUN9_001681"
     CDS             complement(20082..21464)
                     /gene="cusC"
                     /locus_tag="ACHUN9_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0695099.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21621..22304
                     /gene="cusR"
                     /locus_tag="ACHUN9_001682"
     CDS             21621..22304
                     /gene="cusR"
                     /locus_tag="ACHUN9_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0695100.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22294..23742
                     /gene="cusS"
                     /locus_tag="ACHUN9_001683"
     CDS             22294..23742
                     /gene="cusS"
                     /locus_tag="ACHUN9_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0695101.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5735 a         6484 c         6036 g         5513 t
ORIGIN      
        1 tgggtttata gataatatta atttacctgt ttagcgttgc gcgaataatc ggcccaattt
       61 ttacaaacgc ccctggagag ataatatcca catgcgcaca atcctgacga tagatatcca
      121 gctccgcaat ccacggcgac caggcgcgtt caggactcat accttcctga agcgtgcgtt
      181 cagcaacaaa caacgtcgct ttaccgtcaa acggtacgct atgagcagtc gtcagcaggc
      241 gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa ctccgttgaa gtacttccct
      301 gctgtgccgc caggaaggcc tcgcgctcgc ggttaatctc cgccagcact tccgggtcca
      361 gaccattagc ttctttttcc tgccagtttt gcgtttctgg cggccaggta tccagcaagc
      421 caagaaatgc cacctgttcg ccacgggcac gcagtcgcgc cgcaataccc tgcgccagcg
      481 taccgccaag ggaatacccc agcaggtaat aagggccgtg cggttgttgc tcaagtaacg
      541 ttgccagatg cgcttcgcag acttcatcca ggtttgtcgc tgtctgcatg ggtccatgag
      601 ggcgcggcga ctgaatgcca ataatcgacc attgtggatc gagataacgc gagagtacgc
      661 taaactgcca ggcaaaaccg gacgccggat ggaaacaaaa cagcgtcggg ccattacctt
      721 cacgcaacgg cagaatggtt tcgaatccca tgcgccggga gctgtcctct tcaccatcaa
      781 taatcgttgc cagtttggcg acggttgacg cgaccatcac ctgccccggc gtcacctgac
      841 gagcaaactg tcgacttaac tgcgctgcca gtttcattgc cagtagcgaa tgaccgccca
      901 gcgcaaagaa atcagcatcg gcatcctgca cgtcacaacc cagcaacgac gcgaatgccg
      961 cggcgataat cgtttcactg cccgctttcg gtgcacgtcc cgacgcttgt gtcttcagtt
     1021 caggcaacgg taaggctttg cgatccagct tgccgttggc gctaagaggt aactgtggaa
     1081 gttgcagcag aaccaccggt accatatgtg gtggcaatgt ttcacgaagc tgcgcctgca
     1141 atgcgctggt atccaacggc aggcccgatt gcgacaccag atagcccacc aactgacgcg
     1201 catcaccacc agtggctgcc gcctggttaa tcacacaggc gtgggtaacg gcttgttcga
     1261 catccggcag cgcctgcatc acgcgatcga tttcgcccag ttcgatacgc tgcccgcgaa
     1321 tttttagctg atcgtcactg cgcccgaggt actccaccgc gccgttatcc agccagcggg
     1381 caacgtctcc ggtacggtac atccgttcgc caggagcaaa aggatcggca ataaagcggc
     1441 tggcggtcag atcggggcgt ccgagatagc cctgcgccag ttgaatgccg gtaagataga
     1501 gatcacccgc cacacccggc ggcaccggat gcatcatcgc atcaagaata cgcagcccca
     1561 tgttccacac cggataacca atcggcacac tgctgccgcg cacctgtgcc agttcctcgc
     1621 caaaagccgg ataccagctg acatctaccg ccgcttccgt cgggccatac agattatgta
     1681 acggcgcacc ggttaactgt tgccattcac ggcataaatc agccggtaag gcttcaccac
     1741 tacagaaaac ctgtttcaac gtcgcgcaac tctggcgagc ggtttgcgga gtcagcgagg
     1801 caacaaatgc cgccagcatc gacggcacaa agtgcgtggt cgttacgcca tattcggcaa
     1861 agaattgctg catagcgagc gggtcacggt gcgcttccgg ttcagccatc accagttttg
     1921 cccctgcgat aaacggccag aaaaactccc acaccgagac atcaaaactg cacggcgttt
     1981 tttgggcaac gacatcttcg cctgtaagcg ggtagtgatt ttgcatccat aacaggcggt
     2041 taacgatagc cgtctgcccg accattaccc ctttcggcct gccggtggag ccagaggtaa
     2101 agatgatata agccgtgtgg tgcggttgtg aaagttgcag cggcgcactg ccctgggggg
     2161 taagcggggc gttatagcaa aggcttgtta aattgggaac atcgctaaag cgcggcagtt
     2221 gatcgtcggt ggtaattaac agcgacggac gcgcatcttc cagcatcatt ttcaggcgat
     2281 cgtccggata gccggtatcc agcggtagcc aggccgcgcc agcttcaact attgcatgga
     2341 gtgccagggt caaaaagacc gagcgcggta gtgccaccgc cacgctgtcc cctggtttaa
     2401 cgccgcgctc acgcagcaga ttcgccagcg ccaccacctg ctcgcgcatt tcccgatagc
     2461 tgaacaggta acgcgcatct gccagcgccg gagcatccgg tgtttttgcc gcttgttctg
     2521 ccaccagcgc gctcagcgtg gtttctggaa tctcaacctg agtggcgttg atctgcgcca
     2581 gctgcgcata ctcacctggc agcataatat cgacatcgcc gcacaacagc gccggatccg
     2641 cggcgaactg ggcaatcagc attttcaggc gttcagcatg ctggattaac gttggctcat
     2701 cgtaacgctg tttattggcg aggatctcaa tactcaaatc accgtgttca tccgggaaca
     2761 gggccagttc aaggtcatta accggaccgg ttgccagggt atgggtttgc gcctgaacac
     2821 caggaatatc cagttggtaa tcaaatacct taatattgag taccggacca aacagcggtt
     2881 catcacctgc cgctcgcccg ctgtcacgga caatttgttc ggcatcgtaa cgttgatggc
     2941 gacgcatttt tttcagttga gccgccagtc gggttgccag ttccggcagc gtttcttgcg
     3001 ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg tccgctagcc gtcagcgccg
     3061 ccgagcccag tcgacgcata aagataaatc cggcggcata gtccatgcga ttgcacaatc
     3121 gccccagcca caaggctgcc agcgcaaggg ctaaatcggt acgctgcaca cctgaaagtt
     3181 gcgtagccag ctggcggaat tccccgtcgg taaattccag tttcaggcgc agaatatctg
     3241 ccgaagcgct gcgccccggt aaaggtgccg gagaaagtga cgcgggcggc ggcagttgac
     3301 gacgctgttc tgcccagaat gccgcatcac gctgccaggc ttcgctttcg cggtattgct
     3361 ggtactcttc cactacatca gcgaaaggcg taaacggcga agcaggcgtt ggttcgccac
     3421 gcagccatgt gcagtaaata ttggcgatct ggcgagtaat ggccgggaaa ctgaagccat
     3481 cgaccagcaa atggtgataa cgctgatacc agtaccagcg gttatccgca acatgtatca
     3541 gctgatgaaa gaccagtggt ttaccgctat cgacgcgcaa atcttgttgc aaatccgtca
     3601 gcattaatgc ctgcgcagta ccgtgcggat caatattggt tcgcaggtcg ataatttctg
     3661 gctgttcgaa tatcagcgca tcatcgaccc actgccagac ttcgccgtta tcttccgtaa
     3721 aacgcatccg caacgtatct gcttgcgcta gtccggcaac caccgcgcgg gccagtaatg
     3781 gcgcatcaac atctccagtt aactcaacgt aatgcgccac gctccaggcg gagggtaaat
     3841 ccgacagttt ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt aaatgctggc
     3901 tcattgtgcc tcctgcaact gggtgaaatt ggccggtgtc agagtacgcc agtggacttc
     3961 cagccactgc tggcaggacg cctgtgactg cggctgacac acaatgtccc agcctgccga
     4021 taagaggcat tgttgcggcc acagactgaa ttgcccctgc gcattgcgca aaatgtaaaa
     4081 cgctccctgc ggatcatcga agggattact gaatgccata ctcaactcct gtcatggaaa
     4141 agtggttgcc agaggtcgat taacccctgc atcaagccac cgcgccaaca aagcgcatca
     4201 tgtccgccgt caacctgacg ccagaaaatg gattctttta tagggtgcaa ttgcgcatac
     4261 agcgcctgat tggcccgcat gatcatcggc tcgcgaatac ccgcttccag cacaatgcgc
     4321 agaccttcgg cgctaacttc accagctttt agcttttcaa gtaacacgcc ctcttgctgc
     4381 ccgtcccgat gcggccacca gtacgatcct gactggctta atacacagcc aaagcgttca
     4441 ggccagtgca gtccggcata cagcgcggac agcccaccaa aactctgccc ggcgaccacg
     4501 gtgcgatcgg cgcgatcgct aaaaggggca atagctttca ccaggggtaa taactcttgc
     4561 tgtactgcca gccagaaatc cgcattacac ggcagttcgt gggcgcggtg cgtggtgtcg
     4621 atagcgtcga tcaacacata cacggcggga ggaagttgct gacgatgggt cagcgaagtc
     4681 agcacaggcc agacgggcat actttgcgcc caaaattcgc catcgagcaa aactgccagc
     4741 ggacgttctt ctgctgttac atcgccggtg gtaaaaatcc atacacgacg tgaatttttc
     4801 aaccgttcac ttttccagat aatttctttg gctgctattt ctggcacttg cggacaatcc
     4861 catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg ccctcgcccg
     4921 cctttccagc tttgtggatt cagcggatcg gctattgcct ggggtaatag ttttcgccag
     4981 ccttcacgta attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc gcgttcggtg
     5041 ggaataaagc agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca ctgccagaca
     5101 tcagtacctg caattcgctg catcgactgg ggctggctgt tctgatgatg atcggtcaca
     5161 ccagtgatgt agatccatac gcgctttatc gtcgagtatt cttcagaacc ttggggatca
     5221 cgccaccaga aagtgacctc aaacatttcg tcattcagac gctgccattc cgggccatgt
     5281 ttcgactgcc accagctctc acttcctact tttaacgccg tcacaccata accccatgtt
     5341 tactgtgcaa tttttcattg attgcagaaa tatattgata atattattga taaccatttg
     5401 catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga catttattaa ccaactgcac
     5461 tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga tgcgtcgtgt tgaggacgac
     5521 catgcccgac agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga acaagaagat
     5581 tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg cacaagagcc
     5641 gaccgatact cctgtttcac atgacgatac cattgtcgtt accgccgccg agcagaactt
     5701 acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc ccgttgcccg
     5761 cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac ctgaccggta actccaccag
     5821 tggtcagcgt gggaataacc gacagattga tattcgcggt atgggtccgg aaaacacgct
     5881 gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg gctggcgtgg
     5941 cgagcgcgat acccgtggtg atacctcctg ggtgccacct gaaatgattg aacgtattga
     6001 agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg gcgtggttaa
     6061 catcattacc aaaaaaggca gcggtgagtg gcacggctcc tgggacgctt atttcaatgc
     6121 accagaacat aaagaggaag gtgccactaa acgcaccaac ttcagcctga ccggtccgct
     6181 gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac aaaacccagg ctgacgcgtg
     6241 ggatatcaac cagggccatc agtccgcgcg tgccggaacg tatgccacga cgttaccagc
     6301 cgggcgcgaa ggggtgatca acaaagatat taatggcgtg gtgcgctggg atttcgcgcc
     6361 attgcaatcg ctggaactgg aagcaggtta cagccgccag ggcaacctgt atgcgggcga
     6421 cacccagaac accaactccg attcctatac ccgctcgaaa tatggcgatg aaaccaaccg
     6481 tctgtatcgc cagaactact cgctgacctg gaacggcggc tgggataacg gagtgaccac
     6541 cagcaactgg gtgcagtatg aacacacccg taactcgcgt attccggaag gtctggcagg
     6601 cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata tcgatcttga
     6661 tgacgtgatg ctgcacagcg aagttaacct gccgattgat ttcctcgtta accaaacgct
     6721 gacgctgggt acggagtgga atcagcaacg gatgaaggac ttgagttcca atacccaggc
     6781 gctgaccggg gcgaacaccg gcggcgctat tgatggtgtg agtgccaccg accgtagccc
     6841 gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc tgactgacag
     6901 caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca ataactggag
     6961 cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctcaaaa tgggcattgc
     7021 ccgtgcttat aaagcaccga gcctgtacca gactaacccg aactacattc tctacagtaa
     7081 aggtcagggc tgctatgcca gcgcgggcgg ctgctatcag caaggtaacg atgacctgaa
     7141 agcagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg gctggctggc
     7201 gggcgtcacc tggttccgta acgattatcg caataagatt gaagcgggct atgtggctgt
     7261 agggcaaaac gcagtcggca ccgatctcta tcagtgggat aacgtgccga aagcagtggt
     7321 tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa atggtgatgt ggaccaataa
     7381 catcacttat atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt cgatcatccc
     7441 ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg gaagatttgt cgatgcaaac
     7501 gaccttcacc tggtacggta agcagcagcc gaaaaaatac aactacaaag gtcagccagc
     7561 ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt gtgggtctga gcgcgacctg
     7621 ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac aacctgttcg acaaacgttt
     7681 gtggcgtgcg ggtaatgccc agaccacggg cgatctggca ggggccaact atatcgcggg
     7741 tgccggggcg tatacctata acgagccggg acgtacgtgg tacatgagcg taaacactca
     7801 cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg gatgcggcgt
     7861 gaacgcctta tccggcctac aaaaatcttg ccaattcaat atattgcagg accatgtagg
     7921 cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga atatggtcga
     7981 tatgaaaact acgcatacct ccctcccctt tgccggacat acgctgcatt ttgttgagtt
     8041 cgatccggcg aatttttgtg agcaggattt actctggctg ccgcactacg cacaattgca
     8101 acacgctgga cgtaaacgta aaacagagca tttagccgga cggatcgctg ctgtttatgc
     8161 cttacgggaa tatggctata aatgtgtgcc cgcaatcggc gagctacgcc aacctgtctg
     8221 gcctgcggag gtatacggca gtattagcca ctgtgggact acggcattag ccgtggtatc
     8281 tcgtcaaccg attggcattg atatagaaga aattttttct gtacaaaccg caagagaatt
     8341 gacagacaat attattacac cagcagaaca cgagcgactc gcggactgtg gtttagcctt
     8401 ttctctggcg ctgacactgg cattttccgc caaagagagc gcatttaagg catcggagag
     8461 acaaactgaa gcaggttttc tggattatca gataattagc tggaataaac agcaggtcat
     8521 cattcatcgt gagaatgaga tgtttgctgt gcactggcag ataaaagaaa aaatagtcat
     8581 aactctgtgc caacacgatt aattgacaac atctggtacg attcgcccgc agccatcact
     8641 gactgcgggc gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa ccggatatgc
     8701 agggattgct ccccgcacaa cggctacttc ttcggttcgt aagcgagaac agccttaaac
     8761 tcaatattac gttcctttac ggtgaactca cacagcgagt ctccgaccag aagcgtaaat
     8821 cccagcaccg tcaaacacag cactatgact gccacaaggg catatttcgt cagcatgttt
     8881 atattgcctc catgcgaaga agggactacc atccgagttg ttgaggttcg gagtggcagc
     8941 cccaggattg atagaaatat ctcctggggc ttcatctttc cagacctcag agtaaacctg
     9001 aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcagcaaa atatctgttt
     9061 atttatacag taagtcctgt ttcggacttc tgttggcaac actcgttatt gctcgtaaaa
     9121 cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccctaccat
     9181 cctgacctga caaccggata tgcggggatt actccccgca caacggctac ttcttcggtt
     9241 cgtaagcgag aacagcctta aactcaatat tacgttcctt caccgtaaac tcacacagcg
     9301 agtctccgac cagaagcgta aatcccagca ccgtcaaaca cagcactatg actgccgcaa
     9361 gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact accatccaaa
     9421 ttgttcgggt ttggagtggc agccccaggg ttgatagaaa tatctcctgg ggcttcatct
     9481 ttccagacct cagagtaaac ctgaaaccgg aaagcttcag gcacccgccg ttatcttacc
     9541 cttcctcaac aaaatggctg tttatttata cagtaaatcc tgtttcggac ttctcactcc
     9601 tcatttaatc aactgaagct gagcccgttt tttctgcatg cctcaatcgt caaaattctt
     9661 cgccatcagc cagctctgcc ataacttgcc acattgaaaa agtagccaaa ttgcttcagt
     9721 caccatcatc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
     9781 tctatactga atagaaaacg ccaacataag agaaacctat gccattaccc gattttcatg
     9841 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
     9901 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaataag atcacggccg
     9961 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
    10021 atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg caggcagccg
    10081 cagaccatca tctggaaatt tgcggcggtg gcacacaccc gtttcagaaa tggcagcgtc
    10141 aggaggtatg cgataacgag cgctatcaac gaacgctgga aaactttggc tatctcatcc
    10201 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
    10261 tttatttgct gcacggcttg tcacggtttg tgccgcactt tatcgccctt tccgccgcgt
    10321 cgccatatat gcaaggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
    10381 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
    10441 ttcgctgttt gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
    10501 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
    10561 gccacgcggt aaatatggcg ggattaattc aggctaccgc ccactggtta ctgacagaac
    10621 gcccgttcaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
    10681 gtcgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
    10741 cggaagacac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa attggtgcat
    10801 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
    10861 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
    10921 tctgggccga tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
    10981 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
    11041 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
    11101 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagctagc tttacacttt
    11161 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
    11221 ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
    11281 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
    11341 cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
    11401 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
    11461 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
    11521 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
    11581 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
    11641 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
    11701 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
    11761 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
    11821 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
    11881 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
    11941 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
    12001 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
    12061 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
    12121 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
    12181 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
    12241 cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
    12301 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
    12361 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
    12421 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
    12481 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
    12541 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
    12601 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
    12661 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
    12721 aatcataacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
    12781 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
    12841 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
    12901 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
    12961 tcaggggatt aaactgatcg gcgcgatcct ggtcggcatt ttgatgatct cgctgctgat
    13021 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
    13081 ggtatttaaa gatccgattc ttggtctggt ggccggtatt cagctttccg cgaacgatat
    13141 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcagatggcg cggtgatcga
    13201 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
    13261 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
    13321 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
    13381 aatgcaacgt ctgaataaag cgcatttgtt aaagccttat ttaaccagcc gccatcagga
    13441 aattaatgag tggaatcgcc agcaaggttc tacggagtcg gtattaaacc tgcgccgaat
    13501 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
    13561 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
    13621 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
    13681 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
    13741 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
    13801 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
    13861 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
    13921 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
    13981 cagagatagt ttccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
    14041 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
    14101 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
    14161 aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
    14221 cggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc aaacagcatg
    14281 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
    14341 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
    14401 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
    14461 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttatccgga
    14521 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
    14581 accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc gtagcgccag
    14641 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
    14701 ccgatcatac cgatgattgc cagcacttta atcaacgcaa accagaactc ggtttcgcca
    14761 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
    14821 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
    14881 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
    14941 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
    15001 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
    15061 aatacagccg gacccgccat ctgaatcgcc ggaccaatgc caagaaacag accagtacca
    15121 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
    15181 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
    15241 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
    15301 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
    15361 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
    15421 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
    15481 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
    15541 gtaccgcgcc gtgatacaac gcctcatcca gcttctgctc actgaatgtt tgcggattat
    15601 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
    15661 cggcggcgac cccggcaagg gcaataaaac cggtgcccgt tgccacagaa agatgaaagc
    15721 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
    15781 gcaacgcttc gcccacccga cggaatgcca gatacaacag cacgaagatg atcatcagtg
    15841 tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg aactgcccgg
    15901 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
    15961 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
    16021 gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg gccacgtctg
    16081 ccagggtgat ttgctgcttc atcggcgtca ggatcggtag ctggcgcagt gcctgcgggc
    16141 tatcgcgcca gctttgcgga taacgcagat taatgggata acgggcaatc ccttccaccg
    16201 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
    16261 tcataccgta acgcgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
    16321 ccagacgctc ggctagagca gaagccacgc ttggcaccgt tcgcgctact tcttcaattt
    16381 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
    16441 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
    16501 ccagccccgg cagccgcacg gtgttatcca gttcctcaat gattttgtcc atcgtcatgc
    16561 caggccgcca ctgatcctgc ggcttaagct ggatggtcgt ttcaaccatc tcgagcggag
    16621 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
    16681 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
    16741 ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt aaaaattccc
    16801 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
    16861 tggttttcgg ccagtgcagt actttcagca atagaggatg ataaacacga atcaaaaagc
    16921 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtac cccatcagga
    16981 tagggatcac tacgatcgcc agcagcgccg cgcccgccat cgcataggtt ttagtgaacg
    17041 ccaacgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
    17101 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
    17161 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
    17221 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
    17281 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
    17341 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
    17401 gccagagaaa cagcgcacag actaccgcca ccacgataaa ctcttccagc aacttgccgc
    17461 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
    17521 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggag gcgatcactt
    17581 ctcgggcgtt tttgccggag cgcaggatca ccaccccgcc cgccacttcg ccttcgccat
    17641 tcagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcacgca
    17701 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
    17761 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
    17821 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggctaga ctgatgccat
    17881 attgcgccag gcgctgggga tcgataacca cctgatactc tttcaccacg ccgcccaccg
    17941 acgccacttc cgcaacgtca gggatggttt ttagctcata tttgagaaac cagtcctgca
    18001 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
    18061 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
    18121 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtaccat
    18181 cttcgaaaat gacatacaca taagagtctc caaactgcga gaaaccgcgc acagtcttcg
    18241 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
    18301 gcggtgcctg accgggatag ctggttttaa taatcacctg cacatcggag agatccggca
    18361 gcgcatccac tggcgtgtta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
    18421 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
    18481 attccctcaa tgcgcattgg tagcactttc agagcgcatc cgctccagtg cgccagaaat
    18541 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
    18601 accagagtgt aacgcggtga cgccttgcga tgcctggaaa acggcaacgc gtttcggtac
    18661 aaagcgtccg tcggcatcaa cggtaatcac ccgctgttcg ttgccggtat caatcagcgc
    18721 ctgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
    18781 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
    18841 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
    18901 cgccggaacg gtgagcgtaa actgcgaggc atctttcacc agccaggcga tagactccgg
    18961 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgctacca cgttatcttt
    19021 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggtgcttt
    19081 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
    19141 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
    19201 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
    19261 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    19321 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    19381 gaaactctgg gcaaaagtca gcggtccgcg cgtgacggta gccgttttca cccccagatt
    19441 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    19501 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    19561 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    19621 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    19681 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    19741 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    19801 ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatag tcatctccgg
    19861 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    19921 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    19981 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    20041 ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg ccaaccgccc
    20101 cctaaggcgg tatacaaaga aatttcgtta acctgacggg cataattcag gtcgagtaaa
    20161 gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata acttaccgcg
    20221 ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt gcagcgacgc cagataacgc
    20281 tgctgggcgc tgatttgatc gttcaggctt tgacgtaatg ccagcgcatc tgccacttct
    20341 ttaaaggcgt tctggatttt ctgttcataa ttcactaccg actgctgctg gcgaatttcg
    20401 gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc aattttagga
    20461 ataaaattcc acatcccgct gctagcgtta aataatgaag acagatcgct gctggcggtt
    20521 gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc accaatatta
    20581 gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg caataagatt
    20641 tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgccgctgtt tactgtctgc
    20701 gcttgcggca gtttgccgta gcttcccaat aacagttgca atgcattatt cgcctgcgcc
    20761 agttccccct gacgtttagc gatgtcactg cgggtacttt ctatcacccc gcgagcctgt
    20821 tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa cgcatatgac
    20881 tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc cagttgctga
    20941 ttgaaatagc tttgcgcgac attagaaacc agcagaatat gcaccgcgcg ctgagcttcc
    21001 tcagtggcta aataattttg tcgctcggct tcgctcatgt tctttaagcg accgaaaaaa
    21061 tcgaggtcaa agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc ggtattccct
    21121 ttaagattac cgctccagct gccgctgccc tcgccattga gctgtgggta gcggtcggca
    21181 tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat gcgcaaatcc
    21241 cggttattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac aaaaaaggtg
    21301 cgccagccca cgttctggta gttatctgct gcgttaacca agccgttctg gctgagtgag
    21361 aactgctgcg gcacgggcat tgccgtacgc tgataatccg gtgccaatga acaaccggtt
    21421 agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc ataatttctg
    21481 gtgattttat accgccaact ttactcgcca ggctctgatt ttccggtgac aggaaaatga
    21541 caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct agaattgccc
    21601 gggcaacatg cggaggaaaa atgaaactgt tgattgtcga agatgaaaag aaaaccggag
    21661 aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc gacaacgggc
    21721 tgaatggcta ccatctggcg atgaccggtg attatgacct gataatcctc gatattatgc
    21781 tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ctccgccaat aaagggatgc
    21841 cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg ctggagttgg
    21901 gggccgatga ctatctggtg aagccgttcg cttttgctga actgctggcg cgggtgcgca
    21961 ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt gccgatctga
    22021 tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact ctgaccagta
    22081 aagagtttac tctgctggag ttttttctcc gccatcaggg cgaagtgctg ccccgctcgc
    22141 ttatcgcctc gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc attgatgtgg
    22201 cggtgaagcg gctacgcggc aaaatcgaca acgactttga gccgaagctg attcagaccg
    22261 tgcgcggcgt gggctatatg cttgaggtgc cggatggtca gtaagccatt tcagcgcccg
    22321 ttttcgctgg caacccgtct gacctttttt atcagcctgg ccaccatcgc ggcgtttttc
    22381 gcctttgcat ggatcatgat ccactcagtg aaagtgcatt ttgccgagca ggatattaat
    22441 gatttaaaag aaattagcgc cacccttgaa cgggtactta atcatcctga cgagacgcaa
    22501 gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt gttgatttcc
    22561 ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgccgga tatccgcgag
    22621 tttacgcgtg acgccatacc cgataaagac gctcagggtg gcgaggtgta tctcctttcc
    22681 ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag caactggcgg
    22741 atgattaact tgccggttgg cccgttggtg gacggcaaac cgatttatac gctctacatc
    22801 gcgctttcga tcgattttca tcttcattac ataaatgatt tgatgaataa acttattatg
    22861 accgcatctg tcatcagcat cctgatcgtc tttatcgtgc tattggcggt acataaaggt
    22921 cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa agatctcgac
    22981 gttcgccttg acccgcagac cgagcccatt gagctggaac agctggtact gtcgttcaac
    23041 catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc agcggatatc
    23101 gctcacgaaa ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat cgccctcagc
    23161 cagtctcgca gccagaagga gctggaagat gtgctctact ctaatctcga agagctgacg
    23221 cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa caaccagcta
    23281 atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt cgattttttc
    23341 gaggcgttag cggaagatcg cggcgtggag ttgcgatttg ttggcgacaa atgtcaggtt
    23401 gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa tgccctgcgt
    23461 tatacgccaa ccagagagac aattgtagtg cgctgtcaga cggtcgatca ccaggtgcaa
    23521 gttaccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg attgtttgac
    23581 cgtttctacc gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg cattgggctg
    23641 gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac gtcagatgcg
    23701 cgggggacaa ggtttgtgat tatgctgccg gagagagagt gattattaag ttcaaattca
    23761 gggcgtgg
//
LOCUS       DAZOYS010000049        23390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOYS010000049 DAZOYS010000000
VERSION     DAZOYS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.049"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUN9_001684"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUN9_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0695102.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUN9_001685"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUN9_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0695103.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWLSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUN9_001686"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUN9_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0695104.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUN9_001687"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUN9_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0695105.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUN9_001688"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUN9_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0695106.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUN9_001689"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUN9_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0695107.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUN9_001690"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUN9_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0695108.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUN9_001691"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUN9_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0695109.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUN9_001692"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUN9_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUN9_001693"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUN9_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0695110.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUN9_001694"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUN9_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0695111.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUN9_001695"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUN9_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0695112.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUN9_001696"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUN9_001696"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0695113.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHUN9_001697"
     CDS             complement(14171..14743)
                     /locus_tag="ACHUN9_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0695114.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUN9_001698"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUN9_001698"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0695115.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUN9_001699"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUN9_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0695116.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHUN9_001700"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHUN9_001700"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0695117.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHUN9_001701"
     CDS             17912..18760
                     /locus_tag="ACHUN9_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0695118.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUN9_001702"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUN9_001702"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0695119.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUN9_001703"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUN9_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0695120.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHUN9_001704"
     CDS             complement(21672..21872)
                     /locus_tag="ACHUN9_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0695121.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUN9_001705"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUN9_001705"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0695122.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHUN9_001706"
     CDS             complement(23031..23387)
                     /locus_tag="ACHUN9_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0695123.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5851 a         6221 c         6266 g         5052 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctaagcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat
//
LOCUS       DAZOYS010000050        23004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOYS010000050 DAZOYS010000000
VERSION     DAZOYS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.050"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUN9_001707"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUN9_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0695124.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUN9_001708"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUN9_001708"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0695125.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUN9_001709"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUN9_001709"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0695126.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUN9_001710"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUN9_001710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0695127.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUN9_001711"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUN9_001711"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0695128.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUN9_001712"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUN9_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0695129.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUN9_001713"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUN9_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0695130.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUN9_001714"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUN9_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0695131.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUN9_001715"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUN9_001715"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0695132.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="ACHUN9_001716"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="ACHUN9_001716"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0695133.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUN9_001717"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUN9_001717"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0695134.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUN9_001718"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUN9_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0695135.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUN9_001719"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUN9_001719"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0695136.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUN9_001720"
     CDS             complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUN9_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0695137.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19968..21116
                     /locus_tag="ACHUN9_001721"
     CDS             19968..21116
                     /locus_tag="ACHUN9_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0695138.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21326..22756
                     /locus_tag="ACHUN9_001722"
     CDS             21326..22756
                     /locus_tag="ACHUN9_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0695139.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22757..>23004)
                     /locus_tag="ACHUN9_001723"
     CDS             complement(22757..>23004)
                     /locus_tag="ACHUN9_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0695140.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         5651 a         5725 c         6086 g         5542 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcttggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagaggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttccg ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat gagtaatata
    19861 aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
    19921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    19981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    20041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    20101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    20161 ttaaacgcat tggttttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    20221 gtgccggcct tggcctgatg attggattct ggagttggtt ggcgtggcgt tttgtcgcgg
    20281 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    20341 cacgtaaccg tgggcgtttg cttgctgcat acatgatggt ttattacgtg ggaacgtttt
    20401 taggccagtt actggtcagc aaggtctcaa ccgagctgat gagcgtattg ccgtgggtta
    20461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    20521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
    20581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
    20641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
    20701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    20761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
    20821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    20881 tggcgatggc atgggcttgc gagaaagttg aacatcatca actggtggcg atgaaccaag
    20941 ccttactgtt gagctatact gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    21001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    21061 tgctgatgct gctgcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    21121 attcaagcag agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    21181 ttgctttatt ccaaatttta ttcgtttaaa aataaaatgt gtagcaggtt ataattttgc
    21241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    21301 tataattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    21361 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    21421 ctacgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    21481 cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    21541 ggtcagtacc tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac
    21601 ctactgggtg gtgcatattc cctatctggc acaaaaaccc caggcaattc tgattgcgct
    21661 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    21721 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    21781 gctgaaaatc gtcggttctg tggcagggat tgctatgttt gttatgtcgc tcctgtatgt
    21841 ggcgatggcg gtaaccgcgc ctgcaattac tgaagtgcat attgcgacca caaacattac
    21901 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    21961 tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    22021 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    22081 gggctcgctg gcgatgggga tgatgtttga ttcgcgtaat atcccggatg acttaatgac
    22141 caacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    22201 aatggtgatt tacgccattg cgaataccct gggacaagta gcggcgctgg tattctcgat
    22261 tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt
    22321 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtactggt
    22381 ggcgattctg attatgctgc cgactctcgg cattggtgat atgaacaatc tctataaatg
    22441 gctgttgaac cttaattcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    22501 tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa
    22561 gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac
    22621 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    22681 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    22741 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    22801 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataga
    22861 tctggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa cgaccggtat
    22921 caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg
    22981 catccagttc agcatcaaca tcat
//
LOCUS       DAZOYS010000051        22776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOYS010000051 DAZOYS010000000
VERSION     DAZOYS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.051"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            48..677
                     /locus_tag="ACHUN9_001724"
     CDS             48..677
                     /locus_tag="ACHUN9_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0695141.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            704..2365
                     /locus_tag="ACHUN9_001725"
     CDS             704..2365
                     /locus_tag="ACHUN9_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0695142.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2604..2663
                     /locus_tag="ACHUN9_001726"
     CDS             2604..2663
                     /locus_tag="ACHUN9_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0695143.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2980..3039
                     /gene="ibsE"
                     /locus_tag="ACHUN9_001727"
     CDS             2980..3039
                     /gene="ibsE"
                     /locus_tag="ACHUN9_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0695144.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3159..4592)
                     /gene="hldE"
                     /locus_tag="ACHUN9_001728"
     CDS             complement(3159..4592)
                     /gene="hldE"
                     /locus_tag="ACHUN9_001728"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0695145.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4640..7480)
                     /gene="glnE"
                     /locus_tag="ACHUN9_001729"
     CDS             complement(4640..7480)
                     /gene="glnE"
                     /locus_tag="ACHUN9_001729"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0695146.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7503..8804)
                     /gene="ygiF"
                     /locus_tag="ACHUN9_001730"
     CDS             complement(7503..8804)
                     /gene="ygiF"
                     /locus_tag="ACHUN9_001730"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0695147.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9046..9666
                     /locus_tag="ACHUN9_001731"
     CDS             9046..9666
                     /locus_tag="ACHUN9_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0695148.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9730..10968
                     /gene="cca"
                     /locus_tag="ACHUN9_001732"
     CDS             9730..10968
                     /gene="cca"
                     /locus_tag="ACHUN9_001732"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0695149.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11131..11952)
                     /gene="bacA"
                     /locus_tag="ACHUN9_001733"
     CDS             complement(11131..11952)
                     /gene="bacA"
                     /locus_tag="ACHUN9_001733"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0695150.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12043..12411)
                     /gene="folB"
                     /locus_tag="ACHUN9_001734"
     CDS             complement(12043..12411)
                     /gene="folB"
                     /locus_tag="ACHUN9_001734"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0695151.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12516..13133
                     /gene="plsY"
                     /locus_tag="ACHUN9_001735"
     CDS             12516..13133
                     /gene="plsY"
                     /locus_tag="ACHUN9_001735"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0695152.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13146..14078)
                     /gene="ttdR"
                     /locus_tag="ACHUN9_001736"
                     /gene_synonym="dan"
     CDS             complement(13146..14078)
                     /gene="ttdR"
                     /locus_tag="ACHUN9_001736"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR0695153.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14285..15196
                     /gene="ttdA"
                     /locus_tag="ACHUN9_001737"
     CDS             14285..15196
                     /gene="ttdA"
                     /locus_tag="ACHUN9_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0695154.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15193..15798
                     /gene="ttdB"
                     /locus_tag="ACHUN9_001738"
     CDS             15193..15798
                     /gene="ttdB"
                     /locus_tag="ACHUN9_001738"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0695155.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15847..17310
                     /gene="ttdT"
                     /locus_tag="ACHUN9_001739"
     CDS             15847..17310
                     /gene="ttdT"
                     /locus_tag="ACHUN9_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0695156.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17353..18366)
                     /gene="tsaD"
                     /locus_tag="ACHUN9_001740"
     CDS             complement(17353..18366)
                     /gene="tsaD"
                     /locus_tag="ACHUN9_001740"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0695157.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18603..18818
                     /gene="rpsU"
                     /locus_tag="ACHUN9_001741"
     CDS             18603..18818
                     /gene="rpsU"
                     /locus_tag="ACHUN9_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0695158.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18929..20674
                     /gene="dnaG"
                     /locus_tag="ACHUN9_001742"
     CDS             18929..20674
                     /gene="dnaG"
                     /locus_tag="ACHUN9_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0695159.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20869..22710
                     /gene="rpoD"
                     /locus_tag="ACHUN9_001743"
     CDS             20869..22710
                     /gene="rpoD"
                     /locus_tag="ACHUN9_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0695160.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5506 a         6223 c         5834 g         5213 t
ORIGIN      
        1 tatcccaaag taaatattgc gtcactaaat ggacattgga gtgacatatg attttattcg
       61 ccgactataa taccccttat cttttcgcta tttcctttgt actgttgatc ggccttttag
      121 aaattttagc gcttatctgc ggtcacatgc tctccggtgc tcttgatgca catcttgacc
      181 attataattc cataaccaca ggtcatatta gccaggcact tcattatctc aatatcggaa
      241 gattgccagc tctcgtcgtt ctctgcttgc tggctggttt ttttggtctt attggcatcc
      301 tgttgcagca tgcctgcatc atggtctggc aatcaccact gtcgaacctg ttcgtggttc
      361 ctgtcagttt actgtttacg attattgcgg tgcattatac cggtaaaata gttgcgccct
      421 ggattccacg cgaccatagt tcagctatta cagaagaaga atatattggt agcatggcat
      481 taattaccgg tcatcaagcc acttcaggta atccctgcga aggaaaactc accgatcaat
      541 ttggccaaat acattattta ctactggagc ctgaagaagg aaaaattttc actaaaggag
      601 tcaaagtgtt aattatttgc cgactttctg caacgcgata tttggcggaa aacaatccct
      661 ggccacaaat attgtaattt attaatcaaa aggaaattta ataatggatg atattgttaa
      721 ttctgtgccc tcctggatgt ttaccgcgat tattgccgta tgcattctgt ttattattgg
      781 aattattttc gccaggctct atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg
      841 tttaggtggg caaaaagtgg tgatgagcgg tggcgcaatc gtgatgccga tctttcatga
      901 aataatcccc atcaatatga atactctgaa gctggaagtc agccgctcaa ccattgatag
      961 cctgattacg aaagatcgta tgcgtgtcga tgtagtagtc gctttctttg tacgggtaaa
     1021 accgtctgta gaaggcatcg ccactgcggc gcagacgttg gggcaacgca cgctatcacc
     1081 agaagactta cgtatgttgg ttgaagataa atttgtcgat gccctccgtg caacagctgc
     1141 acaaatgacc atgcatgagt tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa
     1201 tactgtcgca gaagacctat cgaaaaacgg tcttgagctg gaaagcgttt cacttaccaa
     1261 ctttaaccag acctcgaaag aacatttcaa tccgaacaat gcctttgacg ccgaaggttt
     1321 aaccaaactg actcaggaga cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga
     1381 tgtagaagtt gcggtgcgtg agaaaaaccg tgatgcactt tcgcgcaagt tggagattga
     1441 acaacaagaa gcgtttatga cgcttgagca ggagcagcag gttaaaaccc gtactgccga
     1501 acagaatgca cgtattgcgg cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg
     1561 aattctggct gaacgacaaa ttcaggaaac ggagatcgag cgcgagcagg ccgtccgctc
     1621 aagaaaggtt gaggctgaac gtgaagttcg tattaaagag atcgaacagc agcaggtcac
     1681 cgaaatcgcc aaccagacga aatcgatcgc tattgccgcc aaatcggaac aacagtccca
     1741 ggcagaagcg cgtgctaacc ttgcactcgc agaagcagta agtgcgcaac aaaacgtaga
     1801 aaccactcgc cagaccgcag aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc
     1861 gcaggatgca gaaaccaaag cggttgaact gaccgtgcgg gcgaaagcag aaaaagaagc
     1921 cgcagagatg caggcggcgg ctatcgttga gttagccgaa gctacacgta aaaagggtct
     1981 ggcggaagca gaagcacaac gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca
     2041 aaccagcctt aaattcaaac tggccttgtt gcaggcgcta cctgcggtaa tagagaaatc
     2101 cgttgagccg atgaagtcca ttgacggtat caagattatt caggtcgatg gtctgaatcg
     2161 tggcagcgct gcgggtgatg caaacacggg taatgtgggg ggcggaaacc tagcggaaca
     2221 agcattatca gccgctctct cttaccgcac acaggcaccg ctgattgact ccttgctcaa
     2281 tgaaattggc gtttcaggcg gctcactggc ggcattgact tcacccttaa cctcaacaac
     2341 tcccgtcgcc gaaaacgtag aataaatttt cagctatgaa cgtccctggc acaaaaagcc
     2401 ggggattttt tatatctgcg ttccgctaaa aactgcaaat gatcaggccg ttgcaacgtt
     2461 ttgcgtgacc gctcggggaa ggcaaaactg cctctgggaa agcgttgcgc gaggtccggc
     2521 gctcatcaac aatcggggag tcagcaaagg gctgaaacgg gaaagcccct cccgaggaag
     2581 gggccttaca gaaggaaagg gttatgatga agctcgtcat catactgatt gtgttgttac
     2641 tcgtaagttt cgcagcttat taacagccaa tcagaggggg gagaaatcct ccctcaccct
     2701 tgttccttta ctttaggttg aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc
     2761 cctggcccaa aaagccgggg attttttata tctgcgttcc gctaaaaggt gcaaatgctc
     2821 aggacgttgc agcgttttgc gtgatcgctc ggggaaggca aaactgcttc agggaaagcg
     2881 ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa
     2941 gcccctcccg aggaaggggc cttatagaag gaaagggtta tgatgaagtt cgtcatcata
     3001 ctggttgtgt tgttactgtt aagtttcccg acttactaac aactgatcag agggggaaga
     3061 aatcctccct tacccttgtt cctttacttt aggttgaaaa aacaacagcg tcaataggcc
     3121 tgcctgctac aaagcgagat ctgtgaaccg ctttccggtt agcctttttt atcctgttgg
     3181 atcttcttga tgatgttagt ggtcgagcaa ccgtcttcga agttgagcac cagcacttcg
     3241 ccgccgttgg cccagacttc tttactcccg gcgatctctt ctggtttata gtcgccgccc
     3301 ttcaccagta aatctggcag gatcccggcg atcaagcgct gcggcgtgtc ttcttcaaac
     3361 gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg ttcgagcggg
     3421 ttaacggggc gggaatcccc tttcagccgt ttggtggagg catcgctgtt cacagcgaca
     3481 atcagacggt cacccagttt gcgagcattt gccagatagg agacgtgccc ggcgtgcagg
     3541 atgtcaaaga caccgttggt cattaccacc ttttcgccgc gtttacgcgc tgccgctacg
     3601 gccagcttca gttcctcttc ggtcatcacg ccaaagccgg tatctgcgcg cccacgcact
     3661 gcattttcca gctcgatcgg cgaaaccgtg gaggttccca gtttgccgac caccacgcca
     3721 gccgccgcat tggcaaagaa gcaggcttct tccagcgaat tacccgctgc cagcgttgcc
     3781 gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg
     3841 gttggcatat gcagcggcgc tttacccggt tgcagcagcg acataccctg ttcggaacgg
     3901 gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc
     3961 tcttcttcgg tcttacattt accgacaaca gcttcaaatt ccgagagatt cggcgttaac
     4021 agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag caccggaacg
     4081 cccgctttac gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc tttggcgtag
     4141 tcagaaagca ccagcgcgcc aatcgaactc agcgcctgat taatccgctc gtgcagcggc
     4201 tgcggatcaa cgccttcgaa accttcttcg aaatccagac ggatcagctg ttggttgcgg
     4261 gaaagtaccc gtaatttggt gatggtcgga tgcgtcggta cagaaacgaa gtcgcatttg
     4321 acgttgacgt cggccagaga tttactcagc gcgcgcgctg catcgtcaat gcccgtcaac
     4381 ccgaccaggc gtgcattagc accgagagaa gcgatattca tcgccacgtt agccgcgccg
     4441 cccggacgtt cttcgatggt attcacttta accacgggca ccggcgcttc cggcgagata
     4501 cggctggagg gaccgtacca gtaacgatcc agcattacat caccaaccac catcactcct
     4561 gcacgttcaa actctggcag cgttactttc attcctgtct cctgagagat tcaaaatttg
     4621 cgcgcgataa taccatactt cattcttcca ccagccactt ctgccagctt gcccgcacca
     4681 gatcacgctc tgcggtgaag caatcccccg acacatggcc cggcaattcc tgtaatgcca
     4741 gatgatgaag ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc gcttcctgct
     4801 cttccataat gtcgttttgc gccagcagct ccagaatacg cacgttgtct gaccagcgcg
     4861 ttaacttcgg tttttcatga gcgtagcgca acaccagata ttgggtaata aattcgatat
     4921 cggtaattcc gccttcatca gctttgatat caaagcgatc gcgatgttta ttgccgagat
     4981 gagcgcgcat tttctcgcgc atttcccgca cttccgtttg cagagttcta ccttcacgcg
     5041 gcagcgtcat aatctcgcga cgcactgcgt caaagtgcgc ggtgagctgc ggatcgccgt
     5101 acactacccg cgcacgcacc agcgcctgat gttcccacgt ccaggcctcg tttttctgat
     5161 aatcggcaaa tgcttctgtg gaggtaacca gcattcccgc cgccccggac gggcgcagcc
     5221 gcgcatccac ttcgtacaaa atgccggaag aggttcgcgt gctgaacagg tgcataatgc
     5281 gttgcgccag acgaagataa aactgtcgtc cgtcgatttc ccgctcgccg tcagtcatcg
     5341 catccattgg gcaatcatgg aggaagatca ggtcaagatc ggaactgtag cctaactccc
     5401 agccgcccag cttgccgtag ccgaccactg caaaaccacg cccttcgcga tcgttgaggt
     5461 gattcggctt accgtagcgg gcaaccattt gaacccacgc ctgctgaacg acggcatcta
     5521 tcatggcttc cgccagccag gttaagtgat cgctcacttt catcaccggt agcgtaccag
     5581 cgatatccgc tgcggcgatg cgtaacagct gcgcctgttt gaactgacgc agcgcctcaa
     5641 gctgttgctc ttcgtcatct tccggcacgc gcagcaaata ctggcgcaac tcatcgcggt
     5701 aggcatcggt cgccgtcggc tggtaaagag tgtttggatc gagcaattca tccagcaata
     5761 atggataacg cgccagctgg ctggcaatca tcggcgatgc ggcacacaga gaaatcaaat
     5821 ggttaagcgc cgcagggaat tcactgagca attctaaata ggtggtgcgg gtaacaatcc
     5881 ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc gtcttcacgc gcacagacat
     5941 cacttagcag atgcggcatc agatggtcga gcacctgacg tcctcgcggc ccgatggtgc
     6001 gcttatccag ctctttgcgg aaatcggaaa tcagcgttag cacctgtttg cgatcatcct
     6061 cgctaagatg cgccagcact ggcgtagtgt catcttcctg caacgcatcc tgccacagct
     6121 cacgccactg ttccgacagc gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt
     6181 cattaaacac ccggcgcaca ttgttcatat gtgcggtcag cgccccggtc agttgcggcc
     6241 agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt aagctcatca gaaggaagcg
     6301 tctgggtttg ttcgtcgtta atgctttgca gcaggttttc cagacgccgc aggaacagat
     6361 acgccactcg caattgttca gcatcgtttt cagaaagcag atgcagcgca gcaatggcgc
     6421 tgagcgttgg cagtaaagag cacgattgca gcgacggttc gcgcccgccg cgaatgagct
     6481 ggaatacctg gacgataaat tcaatttcgc gaataccacc cgcgccaagt ttgatgttgt
     6541 cggtcaaacc gcgccgacgc acttcacggg caatcattcc tttcatgttg cgcagcgact
     6601 gaatcacgct gaaatcgatg taacgacgga aaacaaacgg gcgtagcatc gcacgcaact
     6661 cgttagcata gacgccttcg ctatcgccca taatccgcgc tttgaccatc gcgtaacgct
     6721 cccagtcgcg cccctgctcc tggtaataat cctccaacgc ggcaaagctc agcaccagcg
     6781 ggccactttc gccaaacgga cgcagtcgca tatccacgcg atagacgaag ccatcctgcg
     6841 ttggttgatc cagcacttta atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat
     6901 ccagttcccg gcgtccgccc tgcgtacaac catgttccgg ccaggcaaaa atcagatcga
     6961 tatcagagga gaaattcagt tccccgccgc ccagcttacc catgcctaag atcagcagcg
     7021 gttgcgcttc gccctgcgca ttgcacggcg ttccccactc gcggcagcag gcgtcataca
     7081 gccagtcacg cgccgcaaca atcagcgtct ccgccagata actgagctgc tgcaatatgc
     7141 tctcttcagt aaccagtgcc agcgtttgcg cccatgcgat gcgcaccata atgcgccgcc
     7201 ggaatagccg cagctcgcgc attaacccgg cttcgtcact cacattactg agcgcctcct
     7261 gcaaccatgc agcgtaatgc tgccattcat cggcctgcgg cggttggctt tccagttccg
     7321 tcaaccactc tcgatgcgca attatgctgt cctgcacaaa atcactgaaa gtaagtactg
     7381 acttcgcctg tgcacttagt ggttcctcgg ctaaaggctc tggcagccgc tcaacaacgg
     7441 tctgccagta ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt atccttgcct
     7501 gattaacgtt ttccgctgtg caaccagaac ggttcctgat tgtttgcctc attacggaaa
     7561 tgctcaattt caatgcgttg cccggtcgca atagcgtgac gcagcccctg ccagttctcc
     7621 agccaggcat gcgcgacgac aggatcatag taacccgcca gcaacagtat tgagtcaatg
     7681 tcacgcgtca ggcgtggcaa ctggtcacgg tagagatcgc ctaacggctg gcagaaaacg
     7741 cttttcagtt cagcggcatg gtgggaaaga tggatatcgg caaagcgttt gaaggagtcg
     7801 ctgattttgc cctgcgcttt ggcatctaaa aatggctgcc atgctttgct taccaaccat
     7861 tcggtcaacg ccagcttcgc cattgccgtt tcggtagagt agaccgccgt cacggcagaa
     7921 accgcagaag caatggtcgc ttcgcattgg gtcagcaaat cacgtaagtg agtgctcgct
     7981 ttacgcggca caataccacc gaacagcatc agcgtatgac ggaccaggct aatggctgcc
     8041 agcacctgtt ctttcgccgc atcgttaccg cgtacccaca gttcttcatg atactgccat
     8101 tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt ctacatcggc ttttgccgca
     8161 acatgcaaaa tggtggtcgg tttgatttca cgcgccggat tgccctgcgc cagatgatag
     8221 ccacgcgccg ctttgctcag gctgccctgg cgtaatccgg tttgcgatac cagttggttc
     8281 gccagtttca gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag ctcacagata
     8341 ggttcagcaa attcccccgc tttcacctcc ccctgatcga gagcgatttc aattcggctg
     8401 ccatcgaccg ccaccagcca tttttcgcga taaaaatcgg tgctgaacag cggttgtacg
     8461 cgggaagcga gatcggcagg caattcaccg ttcggccaga cttccgaggg aaactgcgcc
     8521 aggtcgagcg tcggttcgct caacgccacg ttatattccg ggcgctggtg taagccgcct
     8581 gtcactcttc ctgcaacttt catggtcatc tcatagcgac cgttttcgcc acgaatacgt
     8641 aagcccatat cgtgcctacg cagccagtta tccggcgttt cgtagtaaat attcagcaac
     8701 tgcacggggt catggtgctc gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg
     8761 gcactgtgat taacaataaa ctttaattcg atttcctgag ccatggcctt tacttatcgg
     8821 ttatgtcaca taagtgacga tgaacggcga acttaatgcg atcttttttg tcagtagata
     8881 gtattttgcg ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag tttgaagcag
     8941 tggcaagacc tgacacagag gattccgatt gatgtcgaat gctttgcgta gcgacactga
     9001 tgccactact atcgttccac tttccatgac aataacgaca gcctgatgcc aaaattacgc
     9061 ctgatcggat taactttact cgcacttagc gcgactgccg tctcacacgc tgaagaaacg
     9121 cgctatgttt ctgacgaact gaatacctgg gtccgtagcg gtccgggaga tcattatcgc
     9181 ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat tacaaactga cgccaacacc
     9241 aattatgccc aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt gaaacaactt
     9301 agcactgagc caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt caaaaccctg
     9361 accgataaac tcaccaatat cgataacacc tggaatcagc gcaccgcaga aatgcagcaa
     9421 aaagtggcgc agagcgacag cgtgatcaac gggttaaaag aagaaaatca gaaactgaaa
     9481 aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct ggatgacaaa
     9541 cagcgcacca tcatcatgca atggtttatg tatggcggtg gcgtgctggg gcttggcttg
     9601 ctgctcggtc tggtactgcc gcacctgatc ccaagccgca aacgcaaaga tcgctggatg
     9661 aactaaatcg ccttcttcgt tgcactgtct tattattagg aattgtgtat ttgacgaagg
     9721 gatgatggcg tgaagattta tctggtcggt ggtgctattc gggatgcatt gttagggcta
     9781 ccggtcaaag acagagattg ggtggtagtc ggcagtacgc cacaggagat gctcgacgcg
     9841 ggctaccagc aggtaggccg cgattttcct gtgtttctac atccgcaaac gcatgaagag
     9901 tatgcgctgg cacgtaccga acggaaatcc ggttccggtt acaccgattt tacttgctat
     9961 gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc gcgatctgac cattaatgcg
    10021 ctggcccagg acgataacgg cgagattatc gacccgtaca acggtctggg cgatctgcaa
    10081 aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag atccgttacg cgtactgcgc
    10141 gtggcgcgtt ttgctgcgcg ttatgcccac ctcggttttc gtattgccga tgaaactctg
    10201 acgctgatgc gcgagatgac ccatgcgggt gaactggaac acctgacgcc tgaacgggta
    10261 tggaaagaga cggaaagcgc cctcaccacc cgcaatccac aggtgttctt ccaggtactg
    10321 cgcgattgcg gcgcactgcg cgttttattc ccggaaattg acgcactgtt tggcgttccg
    10381 gcccctgcca ggtggcatcc ggaaatcgac acgggtattc ataccttaat gacgctctca
    10441 atggcggcga tgctgagtcc gcaggtcgat gtccgtttcg caactttatg ccacgatctc
    10501 ggtaaaggac tgacgccgcc agaactttgg ccgcgtcatc atggtcacgg tccggcgggt
    10561 gtgaaattag tggaacaact ctgccagcgt ctgcgcgtgc caaatgaaat tcgcgattta
    10621 gccagactgg tggctgagtt tcacgatctc atccacacct tcccaatgct gaacccaaaa
    10681 actatcgtca aattgtttga ttccatcgac gcctggcgta aaccgcagcg tgtcgagcaa
    10741 ctggcgctga ccagcgaggc tgacgtacgc ggcagaaccg gttttgaatc agcggactac
    10801 ccgcaaggcc gttggttgcg cgaagcctgg gaagtggcgc agtcagtgcc gacaaaagcg
    10861 gtcgttgaag cgggatttaa aggtgtggag attcgcgagg agctgacccg acggcggatt
    10921 gcggcggtag ccagttggaa ggaacaacgt tgcccaaagc ctgactgagt ataaaactaa
    10981 acgttaacac gaaccatccc ctcgcccctt cggggagagg gttagggtga ggggaacaga
    11041 ccagcaccgg tgcgaacaat cacccccacc ccggccctct ccctacagag cgagggagaa
    11101 aaccaggcct gctcccgaac cttttctcaa tcaaaagaac acgacgtaca ccgcagccgc
    11161 cacaataaag cgataaatgg cgaacgggat aaacgaaatg cgcttaatca attgcaggaa
    11221 ggttttaatc gctatcagcg ccaccacaaa agcggtgata aacccaacgg caaacatcga
    11281 gatatcgccg gttgtcagga agccccagct tttgtagaga tcgagcgccg ttgcgcccat
    11341 catcatcggt accgccagca ggaacgagaa ctcggaagca gcgtaacggc tcacgcccat
    11401 cagcatccca cctgaaatgg tggccccgga acgggagaaa cccggccaca gcgccagaca
    11461 ctggaaacag ccaatcataa acgcctgacg ataggtcata tcatcaagac ccggcgcacg
    11521 cggctctttc ggcttcaggc attcggcggc aatcagcaac aaaccgccaa cgaccagtgc
    11581 atacatcaca tttatcgggt taaacaatga cttaatcgtg tcgtggaaca acagccccag
    11641 taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac ctttgctttc
    11701 tccttcgtgc tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca gacgccgcca
    11761 gaacatcact actaccgcca gaattgatcc taactggatc acaacttcaa aggttttcgc
    11821 cgtgtcgccc tcgaacccca acaagtgacc gacaataatc atatggcccg tgctggatac
    11881 cggcagaaat tctgtcaatc cttcgaccac acccaatatt gccgctatca gcagcgagtg
    11941 catatcgctc atcaataaac ccctaaatta ttaaaatgta ccgcttgtcc ggactactgc
    12001 agtaagacca ggttataacc gtttggttta acagctgtaa aattaattat tttctttcag
    12061 attattgcca cgctcaatga ttacaccaac attcgccgcc cgcgccattg cgcctggctt
    12121 gctgagtttg atgcgcaccc acggcgagtt gaagcgtgct aacagcagct ccgccacctc
    12181 ttcagccacg cgttccacca gcgcaaaacg cgccccctcg acgtggctga ccaccgtttc
    12241 tgcaatgtca gcgtaactga ggcaatccgc cacatcatca cttttcgccg ctttacggtt
    12301 atcccatgcc atttcgatat cgaacactaa cttctgttcg atggtctgtt cccagtcgta
    12361 aacaccaata gtggtgatta ccgaaagttg ctctataaat acaatatcca tcacgtcctg
    12421 cctgcttttt ggctaaccgg ataccacttc cggcgaaatg tgcgtattat ccacagattc
    12481 atcgttgaac acgaattttc aaaacggaac agcttatgag tgcaatcgcg cctggaatga
    12541 tcctcatcgc gtacctctgc ggctccattt ccagtgccat tctggtttgc cgcttgtgtg
    12601 ggctgcccga tccgcgaacc agcggctccg gcaatccagg cgcaaccaat gtgttacgta
    12661 tcggtggcaa gggagcagcc gtagcagtac tgattttcga cgttctgaaa ggaatgttgc
    12721 ccgtctgggg cgcgtatgaa ttaggtgtca gccccttctg gttaggctta attgccatcg
    12781 ccgcctgtct tggacacatc tggcccgttt tcttcggatt taaaggagga aaaggcgttg
    12841 ctaccgcttt tggtgccatc gcacccattg gctgggatct caccggagta atggcgggaa
    12901 cctggttact gaccgtgcta ttgagcggat actcgtcgct gggagcgatt gtcagtgcac
    12961 tgattgctcc gttttatgtc tggtggttta agccacaatt caccttcccg gtttcgatgc
    13021 tctcttgcct gatcctgctg cgtcatcatg acaacatcca gcgtctgtgg cgtcgtcagg
    13081 agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa ggatcccgag tgatttctgg
    13141 tggatctaca tgacctgata gccttcatcg ggcttgccca gccgttgctg gcaccatgcc
    13201 gccagaaatt ccacgcagac acgtaatttc atgctgcgat agagcggctc ccggtaaaca
    13261 gcccagatat tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc
    13321 agaaacggca acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat
    13381 tgcaacacta tctcgccgct attggaggaa agatgcccgc taaccttcac cgattttttc
    13441 tcctgaccgt tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga
    13501 cagtcatgac gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca
    13561 ggtgctgcgc ataatattct tttatttttc gttaacagat gcgcaatata ataatcagga
    13621 atttcgtcat taatacgaat atccagatca atattatcct gcactaaatc aatttgccga
    13681 tcgaacaatt caaaatgcac ctgtaactca ggataattgc gcataagttc ggtaatggct
    13741 ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg
    13801 cgcgttttga tttgcgtgac gtcatcgacc agtcgctgat attgggtgag gatttccagc
    13861 gcatgttcgt aacaacgttg gccactttca gtcagcgcca cgccgcgagc ggagcggttc
    13921 agcagcgttg ttgccagcgt attttcaagg atttggatcc gcttggtgac aaatgcaggc
    13981 gtctgcccaa gcgtcgccgc tgcggcgcta aaactcccgc tgtggacgat ttctaccagt
    14041 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
    14101 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
    14161 attttcgcgt tgattcacga attgttaatt acatattcac ggtggcaaaa aatataaaac
    14221 cacattttta gtggtagttt gtggcggtga atttttccag acaaatacaa aaactggagt
    14281 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacgg agattgtcgc
    14341 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
    14401 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
    14461 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
    14521 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
    14581 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
    14641 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
    14701 ccccgacaat gacgatgcgg aaatcgaagt ttacatggca ggcggcggct gcacgctacc
    14761 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
    14821 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
    14881 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg
    14941 cagccgccat cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa
    15001 ccgtctgggg attggtccac aagggctgac tggcaacagt tcagtgatgg gcgtacatat
    15061 cgaatctgcc gcccgccatc cgtcaaccat cggcgttgct gtctctaccg gctgctgggc
    15121 gcatcgtcgc ggcacactgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac
    15181 ccggagcgcg ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat
    15241 attcgcgtcg acgatgtgat ctacctgacc ggtacgctgg tgacctgccg tgacgtttgt
    15301 caccgccgct tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt
    15361 ttccacgctg gccccatcgt gcgcaaaaac ggcgataaat gggagatggt ttccgttggc
    15421 ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg
    15481 aaactggtgg ttggcaaagg cggaatgggg ccgctgaccg aagaaggctg ccagaaattc
    15541 aaggcgctac atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa
    15601 gagattgaag aagtgcactg gacagagctc ggaatgccgg agtcgctgtg ggtctgtcgg
    15661 gtcaaagagt tcggcccgct gattgtctct attgataccc acggcaacaa cctgatagcc
    15721 gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac
    15781 gtccattaca tcaaataacc ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat
    15841 aacacgatga aaccttccac tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc
    15901 attattgctc taattccggt tcccgcaggg ctggagagtc atacctggct ctactttgcc
    15961 gtttttactg gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg
    16021 atggtgggta tatccatcat cgccatactc tctccctggc tgctgttcag cccggagcag
    16081 ctcgctcagc caggctttaa attcactgca aaatccctct cgtgggccgt ttccggtttt
    16141 tctaattcgg ttatctggct gattttcgcc gcctttatgt ttggcacagg ctatgaaaaa
    16201 accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa agatgggaca tcgcacgctg
    16261 tttctcggct atgcggtgat gttctccgag ctgatcctcg cacctgtaac accgtccaac
    16321 tcggcgcgtg gtgcggggat tatctatccc atcatccgta acctgccacc gctctatcaa
    16381 tcacaaccaa acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt
    16441 gttgccgact gcgtgaccag cgccattttc ttgacggcga tggcaccaaa cttgctgtta
    16501 attggactga tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg
    16561 atgttgccgc tcagtatttt gctggtgctg ctggttccct ggctggctta tgtgctgtac
    16621 ccgccggtac tgaagtctgg cgatcaggtg ccgcgctggg cagagacgga actgcaggca
    16681 atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc tgatagtagg cgcgctggtg
    16741 ctgtggattt tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggca
    16801 ctgatgctgc ttctgcgcat tatcagttgg gacgacattg tcagtaataa agcggcgtgg
    16861 aacgttttct tctggctggc ctcgcttatc accctcgcta ccggactcaa caacaccggt
    16921 tttattagct ggtttggcaa actgttagca ggcagcttaa gcggttattc gccaacgatg
    16981 gtgatggtgg cgttgattgt ggtgttttat ctgctgcgct actttttcgc cagcgccacg
    17041 gcgtatacct ccgccctcgc accgatgatg attgccgctg cgctggcgat gccggaaatc
    17101 ccgctgccgg tgttctgcct gatggttggc gcggcaattg gtctggggag cattcttaca
    17161 ccatacgcca ccggacccag tccgatttac tacggtagtg gttatctgcc aacggcggat
    17221 tactggcgac tgggggcgat ttttgggctg atattcctcg tgttgctggt gattaccggc
    17281 ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg
    17341 cacccaatag ttttacgcag ccggtaactc cgccagcggc cagcgcggac gcacgctaac
    17401 gccgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc
    17461 gccgttatca gtacaaaact ccggacgcgc gtagaacact tcgccgcggc gttttttcat
    17521 catttcagcc agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag
    17581 tcgcttaaag cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac
    17641 cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg
    17701 aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccggacggtc
    17761 ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag
    17821 taacggtccg ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc
    17881 ggcggcatca tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat
    17941 taactgcgta tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc
    18001 ttccagcatc ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt
    18061 ccaggcaaac gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc
    18121 agggcctgcg gtataggcca cagcatcaat gtcttttgcc gttaagccag actcctttag
    18181 cgccgcctgg atcaacggta cggttttgcg cacgtgatcg cgggaggcca gttcaggcac
    18241 gacgccgcca tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc
    18301 tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac
    18361 acgcatgact tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat
    18421 gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc
    18481 cctttcaaaa aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc
    18541 attttgaaat aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc
    18601 acatgccggt aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca
    18661 agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa
    18721 aaccgactac cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac
    18781 tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc
    18841 gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc
    18901 gtttatgaat tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa
    18961 tgatctgctg gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa
    19021 gcagggcaag aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac
    19081 cgttaacggt gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat
    19141 cgacttcctg atgaactacg acaagctcga gttcgttgaa acggtcgaag agctggcagc
    19201 aatgcacaat cttgaagtgc catttgaagc aggcagcggc cccagccaga tcgagcgcca
    19261 tcagaggcaa acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt
    19321 acaacaacct gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga
    19381 ggttatcgct cgctttgcga ttggttttgc gccccccggc tgggacaacg tcctgaagcg
    19441 gtttggcggc aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa
    19501 cgatcaggga cgcagttacg atcgtttccg cgagcgggtg atgttcccca ttcgcgataa
    19561 acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc aacgataccc ccaaatacct
    19621 gaactcgccg gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc
    19681 gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt
    19741 ggcgctggcg caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc
    19801 cgatcacata caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga
    19861 ccgtgcaggc cgcgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga
    19921 cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg
    19981 aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag gcgatgccgc tttccgcatt
    20041 tctgtttaac agcctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt
    20101 gagtacgctg gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct
    20161 tcgtcaggaa ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat
    20221 gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat
    20281 gcgtatactt atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct
    20341 tgagaatctg gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac
    20401 ttgtctctcc cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa
    20461 taatgctgcc acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc
    20521 tgagcaaacc ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca
    20581 ggaagagtta atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct
    20641 ctggacatta aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg
    20701 atcgggaagc ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg
    20761 taattatcgt tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg
    20821 gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac
    20881 ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat
    20941 gccgaggtca atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc
    21001 atccaaatga tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat
    21061 ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt
    21121 tccagcgtgg aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa
    21181 atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa
    21241 gacgggatca accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg
    21301 ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc
    21361 tttgttgacc cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag
    21421 ctttcccagg aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac
    21481 agcgccgatg atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaacta
    21541 cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc
    21601 gctcaggaag agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag
    21661 cagtttgact acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa
    21721 cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc
    21781 ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag
    21841 ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg
    21901 cagcagattg aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt
    21961 atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta
    22021 cgtctggtta tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg
    22081 attcaggaag gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt
    22141 tacaagttct ccacctacgc aacctggtgg atccgtcagg cgatcacccg ttctatcgcg
    22201 gatcaggcgc gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac
    22261 cgtatttctc gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct
    22321 gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca
    22381 atctccatgg aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag
    22441 gataccaccc ttgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg
    22501 cacgacgtgc tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt
    22561 atcgatatga acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc
    22621 gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct
    22681 gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca
    22741 cccggcacta gtccctctgc acaaacgcca cctttt
//
LOCUS       DAZOYS010000052        22756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOYS010000052 DAZOYS010000000
VERSION     DAZOYS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.052"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..475)
                     /gene="folK"
                     /locus_tag="ACHUN9_001744"
     CDS             complement(<1..475)
                     /gene="folK"
                     /locus_tag="ACHUN9_001744"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0695161.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSK"
     gene            complement(472..1869)
                     /gene="pcnB"
                     /locus_tag="ACHUN9_001745"
     CDS             complement(472..1869)
                     /gene="pcnB"
                     /locus_tag="ACHUN9_001745"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0695162.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1929..2825)
                     /gene="gluQRS"
                     /locus_tag="ACHUN9_001746"
     CDS             complement(1929..2825)
                     /gene="gluQRS"
                     /locus_tag="ACHUN9_001746"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0695163.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2892..3347)
                     /gene="dksA"
                     /locus_tag="ACHUN9_001747"
     CDS             complement(2892..3347)
                     /gene="dksA"
                     /locus_tag="ACHUN9_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0695164.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3525..4229)
                     /gene="sfsA"
                     /locus_tag="ACHUN9_001748"
     CDS             complement(3525..4229)
                     /gene="sfsA"
                     /locus_tag="ACHUN9_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0695165.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4244..4774)
                     /gene="thpR"
                     /locus_tag="ACHUN9_001749"
     CDS             complement(4244..4774)
                     /gene="thpR"
                     /locus_tag="ACHUN9_001749"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0695166.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4848..7277
                     /gene="hrpB"
                     /locus_tag="ACHUN9_001750"
     CDS             4848..7277
                     /gene="hrpB"
                     /locus_tag="ACHUN9_001750"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0695167.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7473..10007
                     /gene="mrcB"
                     /locus_tag="ACHUN9_001751"
     CDS             7473..10007
                     /gene="mrcB"
                     /locus_tag="ACHUN9_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0695168.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10227..12416
                     /gene="fhuA"
                     /locus_tag="ACHUN9_001752"
     CDS             10227..12416
                     /gene="fhuA"
                     /locus_tag="ACHUN9_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0695169.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12467..13264
                     /gene="fhuC"
                     /locus_tag="ACHUN9_001753"
     CDS             12467..13264
                     /gene="fhuC"
                     /locus_tag="ACHUN9_001753"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0695170.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13264..14154
                     /gene="fhuD"
                     /locus_tag="ACHUN9_001754"
     CDS             13264..14154
                     /gene="fhuD"
                     /locus_tag="ACHUN9_001754"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0695171.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14151..16133
                     /gene="fhuB"
                     /locus_tag="ACHUN9_001755"
     CDS             14151..16133
                     /gene="fhuB"
                     /locus_tag="ACHUN9_001755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0695172.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16291..17571)
                     /gene="hemL"
                     /locus_tag="ACHUN9_001756"
     CDS             complement(16291..17571)
                     /gene="hemL"
                     /locus_tag="ACHUN9_001756"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0695173.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17737..17784
                     /gene="yadX"
                     /locus_tag="ACHUN9_001757"
     CDS             17737..17784
                     /gene="yadX"
                     /locus_tag="ACHUN9_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0695174.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17796..19217
                     /gene="clcA"
                     /locus_tag="ACHUN9_001758"
     CDS             17796..19217
                     /gene="clcA"
                     /locus_tag="ACHUN9_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0695175.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19241..19306
                     /gene="yadW"
                     /locus_tag="ACHUN9_001759"
     CDS             19241..19306
                     /gene="yadW"
                     /locus_tag="ACHUN9_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0695176.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19299..19643
                     /gene="erpA"
                     /locus_tag="ACHUN9_001760"
     CDS             19299..19643
                     /gene="erpA"
                     /locus_tag="ACHUN9_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0695177.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19690..20313)
                     /locus_tag="ACHUN9_001761"
     CDS             complement(19690..20313)
                     /locus_tag="ACHUN9_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0695178.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20351..21151)
                     /gene="btuF"
                     /locus_tag="ACHUN9_001762"
     CDS             complement(20351..21151)
                     /gene="btuF"
                     /locus_tag="ACHUN9_001762"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0695179.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21144..21842)
                     /gene="mtnN"
                     /locus_tag="ACHUN9_001763"
     CDS             complement(21144..21842)
                     /gene="mtnN"
                     /locus_tag="ACHUN9_001763"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0695180.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21926..>22756
                     /gene="dgt"
                     /locus_tag="ACHUN9_001764"
     CDS             21926..>22756
                     /gene="dgt"
                     /locus_tag="ACHUN9_001764"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0695181.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5146 a         5798 c         6363 g         5449 t
ORIGIN      
        1 atttgcttaa ttcgtcaaat gctcttgtat gtaagacttc acgcaacgtc tccccatcag
       61 gaaacgccag ctccggtgca atctcaaaca gcggccacag cataaatcca cgattcttca
      121 tatcgtagtg cggaacggtc aggcgttcgg tatttatcac ttcattacca aacagcatga
      181 tatcgagatc cagcgtgcgt ggtccccagc gttcagcttt acgaacacga ccctgttgca
      241 gttctatgcg ctgtgtatgg ttgagtaact cttccggggc gagcgaagtt tcaagcgcca
      301 cggcggcgtt gaggtaatcg ggttgatctt gcggccccag cggtggagtg cggtaaaacg
      361 aagaaacggc aagaatgtgg ctttcaggga tatcgcctaa tgctttcagg gcagcattga
      421 cctgctccag cggagaggcc agattgctgc ctatggcaat atacgccact gtcatgcggt
      481 accctcacga cgcggtgcgc gtttgcgtgg acgacgagta cggcgacgcg gtgacggctc
      541 ttcatccaac tcgttaagca tccctttttg atctggtggc gcggaaacct ggaactcacc
      601 ccaccatttc accagacgct gcagttcagc gttacgttca acttcagctc gcaaggccaa
      661 caggtcataa gccgcacgga acttaggatg ctccagcagt ttccatgcgc gtttaccctg
      721 acgtcgggac atacgcaact gcaactgcca gatatcgcgg gttaacgtcg tcaggcgttt
      781 cgggattgcc agtgaacggc aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc
      841 gtcgtgatag gtcaggccgc tttcctgggc gatcttctgt gccgtctcca gcagtgggta
      901 ccagaacatg gcggcaaaca agaacgccgg gttcacgcgc atatcgttat ggatacgcgt
      961 atcggtattc ttcagcacct gttcaatgat ccgctccatc gggctgtcgc cattttccgt
     1021 gaagtagcgg gtaatggtcg ggaacagcgg ctggaacaga tgatattcac acaacagctt
     1081 ataggtttcg taaccgtagc ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc
     1141 cggtgggata tcgttcagca gggtagcgag gcgagggatc ggttctgcgg tttccgggct
     1201 gatgcgcata cccaatttgg cggcaaaacg taccgcgcgc agcatccgta ccggatcttc
     1261 acggtagcgc gtttccgggt taccaatcag acggataacg ccgtccttca gatccttcat
     1321 accgccaacg taatcacgga cggtaaaatc cgctacgctg taatagaggc tgttgatagt
     1381 gaaatcgcgg cgctgggcgt cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat
     1441 gccgttttgc ccgcgttggg aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg
     1501 gaaggtcgca acttcgataa tctccgggcc aaacattaca tgagccagac ggaaacggcg
     1561 acccaccagg cggcagttac ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt
     1621 tacgtcaaaa tctttcggct ttttgccaag taacaggtcg cgcacgccgc cgccaaccag
     1681 ccaggcttcg tatcccgctt tgttgagcct gtacattacc ttcagggcat tttcactgat
     1741 atctttgcgg gaaatagcat gctgctcacg cgggatcacc gtcacctgtg gacgggcgac
     1801 tgcctgttca gcctcgcttt cctcgcggct tagcaccttg cggcaaaaat tagcgactcg
     1861 ggtaaaaata gtacacctcg gtagtgtcaa acatcattca ggacaaaaaa atagcggcta
     1921 atcatagctc agcatgacgc atttgagaat gttgaattta caattgccga ctcgggcacg
     1981 gcggtcagcg tccagttttt gactgctgac tgaaggattt gttcgacgct gaaatcctgc
     2041 cagtgtgctt ctgcctgctg acccagaaat tgaagtgccg cgattagtac cggacgtgga
     2101 tcgcctttcg gcaacgcagg cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta
     2161 agcgccagcg gcagatgaat gtaatctggc actttccagc caaaaagctg gtacagcgag
     2221 atttgcctta ctgttggttc aatcagatca gccccacgca ctatttctgt aacgccctgg
     2281 aaatgatcat caaccacaac agccaggttg taggcgaaca acccatcacg gcgatgaatg
     2341 ataaaatctt cccgtgccag tttttcgtcg gcgtgaataa tgccgcgcag ctggtcagta
     2401 aattgcgtga ccggatgctg ctggcggata cgcactgcgg cattgtctgg tccatgatgc
     2461 aacacccggc aatgaccgtc gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag
     2521 gtgcagtaat aacttagtcc ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg
     2581 tgacgttgcg attgccagag aacatcgccg tcccagtgca gaccgtaatg ttccagctgg
     2641 cgcaggatag tttctgcggc accgggaact tcacgaggcg ggtcgatatc ttctatgcgt
     2701 accagccagc gaccttgccg ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga
     2761 gagccaaaat gaagctcgcc ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct
     2821 gtcatctctt tgaacaaaaa ataaggcggg agcatttccc gcctgtggta aacgtgatgg
     2881 aacggctgta attagccagc catctgtttt tcgcgaattt cagccagcgt tttgcagtcg
     2941 atgcacagat cggctgtcgg gcgcgcttcc agacggcgaa taccaatttc aacaccgcag
     3001 gattcgcagt agccgaaatc ttcgtcttcc acttttttca gcgtcttctc gatctttttg
     3061 atcagcttac gctcgcgatc gcggttacgc agttcgaggc tgaactcttc ttcctgggct
     3121 gcacggtcta ccgggtccgg gaagttggct gcttcatcct gcatatgtgt aacggtgcga
     3181 tcgacttcat ccctgagttg attacgccat gcttccagaa tacgacggaa gtgcgccagc
     3241 tgggcttcat tcatatactc ttcgcccggc ttctcctgat atggttccac cccagcgatg
     3301 gcgagaatac tcagggacga tgttttacgg ttttgccctt cttgcatgtt gcttctcctt
     3361 aacacgcact atcgatcccc atgttcgggg gaaaaatgag gccgctataa atagcagatg
     3421 cttttccgga tagcaattat ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat
     3481 ttgcgcacgc gaccagaatg taaattaacc agttacttac tttactacaa tgtaaccgac
     3541 agtgattttt taagagccat gccttcagca gaaatttccg ctttgtaagc cagaatttct
     3601 acccccctct gttgagcttc tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg
     3661 ggtgaaaacc gtgtaatggc tgaatgcagc acggcgaaaa agataaccgc acgctggcct
     3721 tcagccgcta cgttcatcaa ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg
     3781 ggaaaatatc cctgttcgtt ctccgctaac gtaaccgatt tcacttcaat atagcagtct
     3841 ggacgcgaat ccgcctgcaa cataaagtca atacggctgc gttctgcgcc gtattttact
     3901 tcgcttttca gcgagctata gcctgacagt tctgaaattg attcattaag gatagcctct
     3961 ttcgtcaacc tgttagccca aagcgtattg acgcaaataa atgcgccgct ctggctttga
     4021 gttagttccc aggtgtgtgg gtatttccgt ttggtgttgt ctgaagtcga ataccagacg
     4081 gtatcgccac gcgttgcaca accggtcatc gcacccgtat tcgggcagtg tagcgttaat
     4141 tcgcgaccat cgggtgtgat cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc
     4201 gcgcgctgta gagggggaga gaattccatt gcgacaatcc ttgttattgc gttagcgccc
     4261 agcgttttag cggcgtgtag cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga
     4321 actccgtcac cgcataagac cagttaaaac ctggcggcgg gattgtcacc gcctcgctgg
     4381 cgtcgcgcaa taaggtaata tgtggatgaa acggacgatt gctttgaaaa caaccgctgc
     4441 gggcagcctg tgaacggagc atattcgcca gctggattaa gccgcgtggc ggctgacgca
     4501 tccctaacca caccacacgc gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga
     4561 aaccaggttg acgaatccgt ccggctaaaa gagaaagcgc cttctctttc tcggcgctca
     4621 cttcgcctaa aaatgccaga gtcagatgca aattatcggc ggcgaccgga cgtcccgcct
     4681 caggtgggaa gtgtgtggcg cgccagtgga taatctgttc gcggatttct gcaggtaagt
     4741 cgatagcaaa gaacagacgt tgcggttcag acatgtgagg cactcggtta tgaattaccg
     4801 cgatgctaca atgtggcgcg aagaatgtta accctctgga gcgttttgtg tcgtcgttgc
     4861 ccgttgctgc cgtcttacct gaattactta ccgctctcga ttgtgcgccg caggtattat
     4921 taagtgcgcc gaccggggcc gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc
     4981 ccggcattaa cgggaaaatt atcctgctgg agccgcgtcg tctggcggcg cgtaacgtcg
     5041 cgcaacggct ggcggagctg cttaacgaaa agccaggcga taccgttggc taccggatgc
     5101 gtgcgcaaaa ctgcgtcggg ccaaataccc gtctggaagt agttaccgaa ggcgtgctga
     5161 cgcggatgat ccagcgtgac ccggagttga gcggtgttgg attggtgatc cttgatgaat
     5221 ttcatgagcg cagcttgcag gcggatttgg cgttggcgct gttactcgat gtgcaacaag
     5281 gtctgcgtga tgaccttaaa ctgctgatta tgtcggctac gctggataac gaccgcttgc
     5341 agcaaatgct gccagaagcg cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac
     5401 gccattattt accgctgccc gcgcatcagc gttttgacga agccgttgcg gtagccaccg
     5461 ccgaaatgct gcgtcaggaa agcggatcat tactgttatt tttacctggc gtcggagaaa
     5521 ttcagcgtgt gcaggaacaa ctggcttcgc gcatcggcag tgatgtgttg ctctgcccgc
     5581 tgtatggcgc gttgtcgctg aacgatcagc gaaaagcgat cctcccggca ccgcaaggga
     5641 tgcgcaaagt ggtgctggcg accaatattg ctgaaaccag tttaaccatt gaaggtattc
     5701 gtctggtggt ggattgtgcc caggagcgtg tggcgcgttt tgagccgcgc acggggctta
     5761 cgcgactgat tactcaacgc gttagccagg catccatgac gcagcgtgcc gggcgcgccg
     5821 ggcgtctgga gccgggtatc tgcctgcatt taatcgccaa agaacaagca gaacgcgctg
     5881 ccgcgcaaag tgaaccggag atcttacaaa gcgatctttc cggtttgctg atggaattac
     5941 tgcaatgggg atgcagcgat ccggcgcaga tgagctggct ggatcaaccg ccagtagtga
     6001 atctaatggc cgcgaaacgt ctgttacaaa tgctgggggc gctggacggt gaacggctta
     6061 gtgcgcaagg gcaaaaaatg gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg
     6121 ttagcgcgaa gaacgacgac gaagctgcta ccgcggcaaa aattgccgcc attctcgaag
     6181 agccgccact gatgggcaat agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct
     6241 ggcagcaacg tagtcagcaa ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca
     6301 gttcgcttat cgcgccgcta cttgccgggg cgtttgccga tcgcattgct catcgccgtg
     6361 ggcaagatgg acgctatcaa ctggcaaacg gcatgggagc gatgctcgat gccgacgacg
     6421 cgctaagccg ccacgaatgg ttgatcgcac cgttattatt gcagggcagc gcctcgccgg
     6481 atgcgcggat tttactggcg ctaccggtcg atattgatga attagtacaa cgctgtccgc
     6541 agctggtgca gcaatctgac accgtggaat gggatgacgc gcaaggtacg ctgaaagcct
     6601 ggcgtcggct acaaatcggt cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag
     6661 aagacgagtt gcatcaggcg atgcttaatg gcatccgtga taaaggttta agcgtgctca
     6721 actggacggc ggaagcggaa cagctacgct tgcgtttgtt atgcgccgca aagtggttgc
     6781 cggaatatga ctggccagcg gttgatgatg aaagtttatt ggcagcgctg gaaacgtggc
     6841 tgctgccaca tatgacaggc gtacattcac tacgcggcct gaaatcactg gacatttatc
     6901 aggcactacg cggattactt gattggggaa tgcagcaacg tctggatagt gaattgcctg
     6961 cgcattacac tgtgccgacg ggaagccgga tcgccattcg ttatcatgaa gataacccgc
     7021 ccgcactggc ggtgagaatg caggagatgt ttggtgaggc cactaatccg actatcgccc
     7081 aggggggcgt gccgctggtg ctggagttgc tttcacccgc ccaaaggcca ctacaaatca
     7141 cacgagattt gagcgccttc tggaaaggag cgtaccgtga ggtgcaaaaa gagatgaaag
     7201 ggcgttatcc caaacacgtc tggccggacg acccggcaaa cacggcaccg acgcgacgga
     7261 cgaaaaagta ttcgtaaggt tggttttctc cctctccctg tgggagaggg ccggggtgag
     7321 ggcatcagcg cgcacgttca ccctcaccct aaccctctcc ctcaagggag aggggaccga
     7381 tcgagcacaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7441 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7501 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7561 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7621 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctgctgt
     7681 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7741 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa
     7801 tggtcaacct tgaaccagat atgaccatca gcaagaacga aatggtgaag ctgctggagg
     7861 caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     7921 acagcattga gatgatccgc cgtccgtttg atttcccgga cagcaaagaa gggcaggtgc
     7981 gtgcgcgtct gacctttgat ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc
     8041 gtcagttcgg tttcttccgt cttgatccgc gcctgatcac catgatctct tcgccaaacg
     8101 gtgagcagcg tctgtttgtg ccgcgcagtg gtttcccgga cttgctggtg gatactttgc
     8161 tggcgacaga agaccgtcat ttctacgagc atgatgggat cagtctctac tccatcggac
     8221 gtgcggtgct ggcaaacctg accgccggac gcacggtgca gggagcgagt acgctgacgc
     8281 aacagctggt gaaaaacctg ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg
     8341 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8401 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgcttg
     8461 cgagcttgta ttactttggt cgcccggtag aagagctcag cctcgaccag caggcgctgt
     8521 tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg gcgtaacccg aaactggcgc
     8581 tggagcgacg taatctggtg ctgcgtttgc tacaacaaca gcagattatc gatggtgaac
     8641 tgtatgaaat gctcagcgcg cgtcctctgg gcgtacagcc gcgcggtggg gtaatctctc
     8701 cacagccagc ctttatgcaa ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg
     8761 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     8821 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     8881 ttgaaactgc aattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtgggag
     8941 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     9001 cccttgcaaa accagcgact tatctgacgg ctttaagcca gccgaaaatc tatcgcctga
     9061 acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9121 cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9181 cccgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcagtta
     9241 cggagacctg gatcaaactg ggcgtgccga aagatcagct gcatccggtt ccggcgatgc
     9301 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcattccag accatcgcca
     9361 gcggcggtaa ccgtgcaccg ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg
     9421 tgctgtatca gagcttcccg caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga
     9481 cactatggac catgcagcag gtggtacaac gtggtacggg tcgtcaactt ggggcgaaat
     9541 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9601 ttgcgggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9661 cgaccaaact gtatggtgcc agcggggcaa tgtctattta tcagcgttat ctggctaacc
     9721 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
     9781 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
     9841 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
     9901 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
     9961 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
    10021 aataccggat ggcgaattgc catccggtaa aataacatcc cacctaaagt attaaccctt
    10081 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10141 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc
    10201 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10261 cactgcgtaa aatcgcagtt gtagtagcca cagcggttag cggcatgtct gtttatgcac
    10321 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10381 aaagcgcatg ggggcctgct gcaactattg cggcgaagca ctctgctact gcgactaaaa
    10441 cggatacacc aattgaaaaa acgccgcagt ctatttcggt tgtgacaaat gaagagatgc
    10501 agatgcgtca atttcagtct gtaaaagaag cattaggtta tacacctggc gttacggtta
    10561 gcagtcgtgg tgcttctaat acatatgatt ttgttatcat tcgtggtttc tcatctgttg
    10621 gtctgaatca aaataattac cttgatggac taaaacttca gggcaacttt tataacgatg
    10681 ctgtgattga tccttacatg cttgagaggg ttgaactgat gcgtgggccg acgtctgttc
    10741 tctatggaaa aagtaatcct ggtgggatta tttctatggt gagtaagcgt ccgaccactg
    10801 aacccctgaa agaaattcag tttaaaatgg ggacggataa tctgtttcag accggattcg
    10861 atttcagtga tgcactggat gataacggtg agttctctta ccgtttgacg ggcctcgctc
    10921 gctcaacaaa cgaacagcag aaaaactctg aatctcagcg ttataccatt gctccatcat
    10981 tctcatggcg tccagacgac aaaaccaatt ttactttcct gtcctatttc cagaatgaac
    11041 cagaaacggg ttattacggt tggttgccga aagaggggac cgttgagcca ttgcctaatg
    11101 gtaagcgtct accgactgac ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc
    11161 agaaaatggt gggatatagt tttgaacatg gttttaatga caccttcacc gtgcgtcaga
    11221 atctgcgttt cagtgaaatg aaaacctcac agaaaagtgt ttatggcaca gggattgctg
    11281 gtgatggtca taccctaaac cgcgggacag tggtggataa tgagcgtcta caaaacttta
    11341 gcgttgatac ccaacttgaa agtaagtttg ctacaggtga cgtcgagcat actttgctga
    11401 caggggtaga cttcatgcgg atgcgtaatg atatcaatgc cagctttgga tccgcaccat
    11461 ccatcgatct ttataacaaa tatcaccctg aatactttgc atttggtagc gcagagccat
    11521 accagatgaa tgaaagcaaa caaacaggta tttatgttca ggatcaggcg gaatggaata
    11581 aatgggtatt cactctgggg ggacgctacg attggtctaa gcaagcgacc actgttcgtg
    11641 aaaactctta tacgccgact gaaggttata ttgagcgcaa tgatcatcag ttcacctggc
    11701 gcggtggtgt aaattactta ttcgataatg gtatttcacc ttactttagc tatagccagt
    11761 cctttgaacc gagtgctttc gatctgtgga gcaacccgcg catttcctat aagccatcga
    11821 aaggtgaaca gtatgaagct ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta
    11881 cgggcgcagt ctatcaattg acgaaaacaa ataacctgac agcagaccca acaaacccat
    11941 tagcgcaagt cccagcaggt gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg
    12001 cgttaaatgc caatattaac ttgacggctt cttataccta caccgatgcg gaatacacca
    12061 aagacaccaa tctcaaaggt aaaactccag aacaagtacc ggagcatatg gcatctctct
    12121 ggggggatta taccttcaat gaagggccgc tttctggttt aacattggga acaggtggtc
    12181 gttttattgg ttccagctat ggtgatccgg caaacacttt taaagtgggt agcgcagctg
    12241 taatggatgc tgttgtaaaa tatgatctgg cacgctttgg tatggcggga tccagccttg
    12301 ctgtgaacgt caacaatttg ctcgatcgtg agtatgttgc cagttgcttc cagacttatg
    12361 gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc
    12421 tcttttgggg cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac
    12481 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac
    12541 gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca
    12601 caacggttct ggtaaatcca ctctgctcaa aatgcttggt cgtcatcagc ctccgtcaga
    12661 aggggagatt cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgtttgcccg
    12721 caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12781 ggtggcgatt ggtcgttacc cgtggcatgg cgcgctgggg cgctttggtg cggcagatcg
    12841 cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt
    12901 cgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga
    12961 tagccgttgt ctgttgctcg acgaaccgac ttcggcgctg gatatcgccc accaggttga
    13021 tgtgctggcg ctggtgcacc gtttaagtca ggaacgtggc ctgacggtca ttgccgtgtt
    13081 gcacgatatc aatatggcgg cacgctactg tgattatctg gtcgccctgc gcagtggtga
    13141 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta
    13201 tggcatcccg atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13261 ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct
    13321 ccgttgttat ggcagatgaa taccgcccac gcggcggcta ttgaccccaa tcgtattgtg
    13381 gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg
    13441 gcggatacca tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatt
    13501 gacgtcggtt tgcgtacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt
    13561 atggtctggt cggcaggata tggcccttca tcagaaatgc tggcacgtat tgcgccgggt
    13621 cgcggattta acttcagtga cggcaaacag ccgttggcga tggcgcgtaa atcgctgacg
    13681 gaaatggcag atttacttaa cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa
    13741 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gcgcacgccc gttactgctg
    13801 acgacgctta tcgatccgcg ccatatgctg gtcttcggtc caaacagctt gttccaggaa
    13861 attctcgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    13921 accgccgtca gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat
    13981 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    14041 tttgtccgcg ccggacgctt tcagcgcgta cctgcagtct ggttttatgg tgcgacactc
    14101 tcggcaatgc actttgtgcg cgttctggat aacgccatcg gaggtaaagc gtgagtaaac
    14161 gaattgcgct ttttccggcg ttattgctgg cgctgttagt gattgtcgct acggcgctca
    14221 cctggatgaa cttctcgcag gcgctgccgc gtagccagtg ggcgcaggct gcctggtcgc
    14281 cagacatcga tgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga
    14341 tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtgctgc
    14401 gtaacccgct ggcggagccg acgacgcttg gcgttgctac aggcgcgcaa ctgggaatta
    14461 ccgtcaccac actttgggcg atcccaggtg ctatggcgag ccagtttgcc gcactggtgg
    14521 gcgcttgtgt cgttggctta atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg
    14581 ttacgcttat tctcgcgggg ctggtggtga gcctttattg cggggcaatt aatcagctac
    14641 tggttatctt ccatcacgac cagctgcaaa gcatgttcct gtggagcacc ggaacgctga
    14701 cgcaaaccga ctggggcggc gttgagcgtt tatggccgca gttgctgggc ggcgtgatgt
    14761 tgacgttatt gctacttcgc ccgttaaccc tgatggggct tgatgatggc gtggcgcgca
    14821 atctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg atagtcatca
    14881 gtgcgctgct ggtgaatgcg gtggggatta tcggctttat cggtttgttc gcgccgctgc
    14941 tggcgaaaat gctgggggcg cggcgtttgt tgccacggtt gatgctggcg tcgctgattg
    15001 gggcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    15061 aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgttgc
    15121 cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac
    15181 gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg
    15241 tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg
    15301 aggatttgat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc
    15361 tggcggtggc gggctgtatt attcagcggc tgaccggaaa cccgatggca agcccggaag
    15421 tgctggggat tagctctggc gcggcgtttg gcgtggtgtt aatgctgttt ctggtgccgg
    15481 gtaatgcctt tggctggctg ttacccgcgg gcagtatcgg ggcggcggtg acgctgttga
    15541 tcattatgat cgccgccggc cgcggtggat tttccccaca ccgtatgtta ctggcgggga
    15601 tggcgttaag taccgcattc accatgcttt tgatgatgtt gcaggcgagc ggtgacccgc
    15661 gaatggcgca agtgctgacc tggatttccg gttcgaccta caacgcgacc gatgcgcagg
    15721 tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg taccgccgct
    15781 ggctgaccat tttaccgctg ggtggtgata ccgcacgagc cgtaggaatg gcgctgacgc
    15841 cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta
    15901 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc
    15961 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtattcg
    16021 ctgactggtg tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcga
    16081 cctttatcgg cgcgccatat tttatctatt tgctgagaaa gcagagccgt taatttttcc
    16141 atataacggc cctgtacgcc tggacggata aagcgtttac gcgacaacgg atgcccgatg
    16201 cgacgctggc gcgtcttatc gggcctacaa acggccccga atcgtaggtc ggataaggcg
    16261 ttcacgccgc atccgacaaa ccatgctgga tcacaacttc gcaaacaccc gacgtgcagc
    16321 atcgatggtg ttattgatat cttccatgct gtgcgccacg gacataaagc ccgcttcaaa
    16381 cgctgacggt gccaggtaaa caccttcgtc cagcatcata tggaagaaac gcttaaagcg
    16441 ttccacgtca caggccatca catcctgata gcacgtcacg gactcggcgt cggtaaagaa
    16501 aataccgaac atgccgccaa cgtggttaac gaccagcgga attccggctt cttctgccgc
    16561 ttccagcaga ccttctgcca gacgtgttgt cagctcatcc aacgtttcgt gaacgcccgg
    16621 ttgcgcgact tcattcagac aggcgaaacc ggctgccatc gcaattgggt taccggaaag
    16681 cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca tccattacat cacgacgacc
    16741 accgaatgcg cctaccggca ttccaccgcc gatgattttg cccaggcagg tgagatccgg
    16801 ttccacaccg taataatcct gtgcgccagc cagtgccacg cggaagccgg tcattacttc
    16861 atcgatgatc agcaatgcgc caaattcgtc gcacagcgca cgcagacctg gcaggaagtc
    16921 tggcagcggt ggaacacagt tcatattgcc tgccaccggc tcgatgataa tacaggcaat
    16981 ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc agatcgttat aagtacaggt
    17041 taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc ctaacgtgag
    17101 tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac acccttcaaa
    17161 tttaatgatt ttgtcgcgac cggtaaaacc acgggccaga cggatggcgc tcatcgtcgc
    17221 ctcggtgccg gagttcacca tgcgcaccat atccatggtc gggaccaatt cggtcaccag
    17281 ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag cttaaaccac gctcggcggc
    17341 ttcaatcacg gcattgcgga ttgccggatg gttatggccc agcaccatcg gcccccagga
    17401 accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc cgtccgcttt
    17461 ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg agttcacacc
    17521 gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca tggagggttc
    17581 ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa gttaaatact
    17641 cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta taaaagtttt
    17701 gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag taaaatgccg
    17761 tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact ccctctttag
    17821 aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt cttgagcgcg
    17881 ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt gttgggctgg
    17941 cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg
    18001 tacatactgc tgataattat ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc
    18061 tggcgatgtt tggctacttt ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga
    18121 tcccggaaat tgaaggggcg ctggaagatc aacgtcccgt tcgctggtgg cgtgtattgc
    18181 cggtgaagtt ctttggcggg ctggggacac tcggcggagg catggtgttg gggcgcgaag
    18241 ggccaaccgt gcagatcggc ggtaacattg gccgtatggt gcttgatatt ttccgcctga
    18301 aaggtgacga agctcgccat acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg
    18361 cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt ccgcagtttc
    18421 gctatacgtt aatttcgata aaagcggtat ttattggcgt cattatgtcg accattatgt
    18481 accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct gatgcgccgc
    18541 ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc ggccctattt
    18601 ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta
    18661 ccaaatgggt actgatgggc ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg
    18721 caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg gggaatttca
    18781 gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta ctctgcttct
    18841 cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact gtgctgggaa
    18901 ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcaccttgag gcggggacgt
    18961 ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg ttaacgggga
    19021 tcattctggt tctggagatg accgataact accagctcat tttgccaatg attattaccg
    19081 gtcttggcgc aacactatta gcgcaattta ccggcgggaa accgctatac tcggcgattc
    19141 ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag gccgcatcag
    19201 ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta ttattgggtt
    19261 agaatttgcc caattgccga tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc
    19321 cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct gacgaagata
    19381 acccgaatct gaaattacgc gtgtatatca caggtggcgg ttgcagcggc ttccagtatg
    19441 gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg
    19501 gcttggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt gactataccg
    19561 aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt
    19621 gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta tccggcgcat
    19681 cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg
    19741 aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg ctgtttccag
    19801 tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa tacaggcagt
    19861 tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg gccagtacat cacgaatgat
    19921 cccgccgcca acgccagtaa tgacgaccat acaaaccgcg attaacggac cgacttccgc
    19981 attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg cgtccagcac
    20041 cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct
    20101 ggttaccatt gcaacgacca gatcggtggg atctttcacc caaaataccg ggccgtgatc
    20161 cagcgccatg tcgcgaattg tcccgccgcc tacagcggta accacgccca gtaccagaac
    20221 accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg caaataccgc
    20281 tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga gcacctaaaa
    20341 aggtcaggcg ctaatctacc tgtgaaagcg cattacagag ctgttgtgca gcgaggataa
    20401 tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga attttgagct
    20461 gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta atgacaatcg
    20521 cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga acccggctgt
    20581 ctttaaagat gttttctccg ccacaaactt cgagtacctg gttttgaatc gactcttttc
    20641 cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta ggtttatcag
    20701 catattgcgc tttcaattgc gcgtactgat ccagcaggga ttgcgcggct tgttcggcct
    20761 tatccggttg cggactccag ggggccaatt gacgtaacgc attggcaatt tgttcaatgc
    20821 ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca acctgccgct
    20881 cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca atgcgttcca
    20941 gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga ggatagtcgg
    21001 aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg
    21061 gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg
    21121 cgaccagcgc cctgaacagt gacttagcca tgtgccagtt tctgcaccag tgactcaacc
    21181 atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag atgagactgt
    21241 tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag
    21301 acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa gttgtggcgg
    21361 attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat caggccacgt
    21421 acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca
    21481 gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca
    21541 tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt cagcgttggt
    21601 gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg ttccaacagc
    21661 agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag cgcaacctcg
    21721 gttccattca gttggccggt atagatttcg caaccgccga gactgatagt ttgacggttt
    21781 tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat gccgattttc
    21841 atagatttac tcgcgataag cccgatttga aagcatagtt taccatgcgc ttacggggaa
    21901 gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa aaataaactg
    21961 gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc tgcggatctt
    22021 cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac
    22081 ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga
    22141 agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga aagagcttaa
    22201 attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca ttgttgagat
    22261 gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg aagcagcgat
    22321 aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc ctctgactga
    22381 cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaacagc ttaacgagct
    22441 gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag gcattcgtct
    22501 ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta ttttaaaata
    22561 tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt taatgaaaaa
    22621 gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag aacttaattt
    22681 ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg acatctccta
    22741 ttgtgtggca gacctt
//
LOCUS       DAZOYS010000053        22681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOYS010000053 DAZOYS010000000
VERSION     DAZOYS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.053"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHUN9_001765"
     CDS             complement(<1..223)
                     /locus_tag="ACHUN9_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0695182.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUN9_001766"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUN9_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0695183.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUN9_001767"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUN9_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0695184.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHUN9_001768"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHUN9_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUN9_001769"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUN9_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0695185.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUN9_001770"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUN9_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0695186.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUN9_001771"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUN9_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0695187.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUN9_001772"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUN9_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0695188.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUN9_001773"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUN9_001773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0695189.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUN9_001774"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUN9_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0695190.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHUN9_001775"
     CDS             7306..8433
                     /locus_tag="ACHUN9_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0695191.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUN9_001776"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUN9_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0695192.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUN9_001777"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUN9_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0695193.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUN9_001778"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUN9_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0695194.1"
                     /translation="MSDINNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUN9_001779"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUN9_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0695195.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUN9_001780"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUN9_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0695196.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUN9_001781"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUN9_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0695197.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUN9_001782"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUN9_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0695198.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUN9_001783"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUN9_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0695199.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUN9_001784"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUN9_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0695200.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUN9_001785"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUN9_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0695201.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHUN9_001786"
     CDS             14427..15242
                     /locus_tag="ACHUN9_001786"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0695202.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUN9_001787"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUN9_001787"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0695203.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHUN9_001788"
     CDS             complement(17104..17286)
                     /locus_tag="ACHUN9_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0695204.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHUN9_001789"
     CDS             complement(17365..18282)
                     /locus_tag="ACHUN9_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0695205.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHUN9_001790"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHUN9_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHUN9_001791"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHUN9_001791"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0695206.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHUN9_001792"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHUN9_001792"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0695207.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHUN9_001793"
     CDS             complement(21301..21957)
                     /locus_tag="ACHUN9_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0695208.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHUN9_001794"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHUN9_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0695209.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5567 a         5949 c         5722 g         5443 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgcttgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacataa
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    22441 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    22501 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    22561 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    22621 cttcattaat gtgcgcgtga tcacgcctgc cgtttggcgc aacaatcatt gatactccct
    22681 a
//
LOCUS       DAZOYS010000054        22335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOYS010000054 DAZOYS010000000
VERSION     DAZOYS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.054"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHUN9_001795"
     CDS             complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHUN9_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0695210.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHUN9_001796"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHUN9_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0695211.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="ACHUN9_001797"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="ACHUN9_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0695212.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="ACHUN9_001798"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="ACHUN9_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0695213.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8839..8937
                     /locus_tag="ACHUN9_001799"
                     /pseudo
     CDS             8839..8937
                     /locus_tag="ACHUN9_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHUN9_001800"
     CDS             complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHUN9_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0695214.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHUN9_001801"
     CDS             complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHUN9_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0695215.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9762..10139
                     /locus_tag="ACHUN9_001802"
     CDS             9762..10139
                     /locus_tag="ACHUN9_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0695216.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10292..10843
                     /gene="apt"
                     /locus_tag="ACHUN9_001803"
     CDS             10292..10843
                     /gene="apt"
                     /locus_tag="ACHUN9_001803"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0695217.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHUN9_001804"
     CDS             10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHUN9_001804"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0695218.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12230..12294
                     /gene="dnaX"
                     /locus_tag="ACHUN9_001804"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12950..13279
                     /locus_tag="ACHUN9_001805"
     CDS             12950..13279
                     /locus_tag="ACHUN9_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0695219.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13279..13884
                     /gene="recR"
                     /locus_tag="ACHUN9_001806"
     CDS             13279..13884
                     /gene="recR"
                     /locus_tag="ACHUN9_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0695220.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13994..15868
                     /gene="htpG"
                     /locus_tag="ACHUN9_001807"
     CDS             13994..15868
                     /gene="htpG"
                     /locus_tag="ACHUN9_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0695221.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16049..16693
                     /gene="adk"
                     /locus_tag="ACHUN9_001808"
     CDS             16049..16693
                     /gene="adk"
                     /locus_tag="ACHUN9_001808"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0695222.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16825..17787
                     /gene="hemH"
                     /locus_tag="ACHUN9_001809"
     CDS             16825..17787
                     /gene="hemH"
                     /locus_tag="ACHUN9_001809"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0695223.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHUN9_001810"
     CDS             complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHUN9_001810"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0695224.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18895..20199
                     /gene="gsk"
                     /locus_tag="ACHUN9_001811"
     CDS             18895..20199
                     /gene="gsk"
                     /locus_tag="ACHUN9_001811"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0695225.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHUN9_001812"
     CDS             complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHUN9_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0695226.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22246..>22335)
                     /locus_tag="ACHUN9_001813"
     CDS             complement(22246..>22335)
                     /locus_tag="ACHUN9_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695227.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5439 a         5920 c         5856 g         5120 t
ORIGIN      
        1 ttttatgaat tttatccgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg
      121 attacacgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc
      601 agctctcgta cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagag gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt
     2041 tttctaccac aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagctc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 catcacccac gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca
     3541 tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg
     3841 ctggactggg tcacatcaag gtagatcgga tcaagttgct gcacggttgc cagcgcagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct
     7021 tccgctgtgg gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggaacag gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag catcacgctc tggcattaca acggcactga agctggcgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg
     8041 aatatgcgtc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcgttt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg actacaaagg cgatgacccg
     8761 acgccagcgg tagggtaata accaaagggg gcgacattta gttgccccga gattactgac
     8821 aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc
     8881 aagaattcaa tgtattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt
     8941 ttgcgtttgc ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg
     9001 cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca
     9061 gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg acagccagtt
     9121 tgacatcgtc aggggcattt tcaagtgaca tactctctcc attgctagcg ggttaagcgc
     9181 gctagcctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag
     9241 gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg
     9301 gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt
     9361 ttacgctgcc agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg
     9421 gaggcttcac gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact
     9481 tgcggcagct gttgctgctc aaccgcatga caaagcgcag ccagattatc gcccgcctcg
     9541 tcgagacaag cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca
     9601 cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct
     9661 tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg
     9721 ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa
     9781 tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac
     9841 cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc
     9901 acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc
     9961 atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca
    10021 tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct
    10081 gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa
    10141 gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca
    10201 caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgtttaa aacgcgccgt
    10261 gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta
    10321 tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga
    10381 tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga
    10441 gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt
    10501 tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa
    10561 actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga
    10621 gatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc
    10681 aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc
    10741 tgacgctgcg ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca
    10801 gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg
    10861 ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt
    10921 gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat
    10981 caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat
    11041 gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt
    11101 tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac
    11161 tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag
    11221 caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat
    11281 acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat
    11341 ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt
    11401 gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg
    11461 gtgacgattt tgtcgcgctg tttgcaattt catctcaagg cgctggatgt cgagcaaatt
    11521 cgccatcaac ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgttg
    11581 caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag
    11641 gcgattgcca gcggtgacgg ccaggtttca acccaggcag tcagtgcgat gctgggtacg
    11701 cttgatgacg atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc
    11761 gtaatggcgc tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg
    11821 gaaatgctcg gcctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc
    11881 aacgacatgg ccgccattga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg
    11941 gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg
    12001 gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg
    12061 ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgtgcc aacggcagta
    12121 atgacgccaa cccaggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc
    12181 ccggaaacca ccagccaggt gctggcggcg cgccagcagt tgcagtgcgt gcagggagca
    12241 accaaagcaa aaaagagtga atcggcagcc gctacccgcg cgcggccggt gaataacgct
    12301 gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg
    12361 ctggaaaaag cgtcagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg
    12421 cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa
    12481 acgccggaac tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca
    12541 caggtgagcc aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa
    12601 gaggagagcg acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac
    12661 cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa
    12721 ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg
    12781 atatacgaag aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag
    12841 accctgcgtc ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg
    12901 taagcacagc ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa
    12961 aggcggtctg ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat
    13021 gcaggaagag atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt
    13081 gaccatcaac ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga
    13141 cgacaaagag atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat
    13201 tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg
    13261 ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg
    13321 cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag
    13381 cgcgatcgta gcggcgggat gcgtttggcg caggcgctca cccgggcgat gtcggaaatc
    13441 ggccactgcg ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat
    13501 ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc
    13561 attgagcaga cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg
    13621 ctggacggca tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag
    13681 gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct
    13741 aactacattg ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc
    13801 gtaccggttg gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc
    13861 gggcgtcata agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa
    13921 ttattctccc ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt
    13981 gaggtagacc tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct
    14041 tctgcacctg atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat
    14101 ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct
    14161 gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct
    14221 gaccatctct gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac
    14281 tatcgctaaa tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga
    14341 cagccagctg atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa
    14401 agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc
    14461 ggctggcgaa ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat
    14521 caccctgcat ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat
    14581 catcagcaaa tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa
    14641 agacggcgaa accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg
    14701 taacaagtcg gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga
    14761 ctttaatgat ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag
    14821 cctgctgtac attccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg
    14881 cctgaaactg tacgtccagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa
    14941 ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg
    15001 tgaaatcctc caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt
    15061 gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca
    15121 acagtttggc ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat
    15181 cgccaaactg ctgcgttttg cttctaccca taccgattct tctgcgcaga ccgtatctct
    15241 ggaagactac gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcaccgcaga
    15301 cagctatgcg gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga
    15361 agttctgctg ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga
    15421 cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga
    15481 agttgatgag agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa
    15541 agccctgctc ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc
    15601 agcgatcgtt tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc
    15661 ggcgggccag aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact
    15721 ggtgaaacgt gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct
    15781 gctggatcag gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg
    15841 tcgtatgaac cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc
    15901 atttttcttt tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagcct
    15961 caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa
    16021 atcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc
    16081 ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac
    16141 tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga
    16201 cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg
    16261 cattgctcag gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc
    16321 gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt
    16381 accggacgaa ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg
    16441 tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga
    16501 agaactgact acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata
    16561 ccatcagatg actgcaccgc tgatcggcta ctacttcaaa gaagcggaag cgggtaacac
    16621 caaatacgcg aaagttgacg gcaccaagtc ggttgctgaa gttcgcgctg atctggaaaa
    16681 aatcctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc
    16741 aggcaatacc tctcacagca attagttctt cttcctcact tttccgctac aattatcaac
    16801 aagttgaatc gataagaggc ggtaatgcgt cagactaaaa tcggtatcct gctggcaaac
    16861 ctgggtacgc ccgatacccc cacacctgaa gcggtaaaac gctatctgaa acaattttta
    16921 agcgacagac gcgtggttga tacctcacgg ttgttatggt ggccattgct gcgcggcgtt
    16981 attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg cctcggtctg gatggaaggt
    17041 ggctcgccgc tgatggttta cagccgccag caacagcagg cgctggcaca acgtttaccg
    17101 gagacgcccg tagcgctggg aatgagctac ggctcgccat cactggaaag cgccgtcgat
    17161 gaactcctgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc
    17221 tgttctacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc
    17281 attccgggga tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg
    17341 gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct
    17401 tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc
    17461 acaacgactc gcgaactggc ttccgcactg gggatggcac cggaaaaagt gatgatgacc
    17521 tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa
    17581 atgctcggag aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat
    17641 tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc
    17701 gggaaaaaat atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg
    17761 gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga
    17821 agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct
    17881 gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg
    17941 ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga
    18001 gtgagatcat tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa
    18061 tacgcctctt catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac
    18121 agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca
    18181 attttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg
    18241 gcacctgcgg aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc
    18301 tgctggtgga aataacaaca agcagccaca atttcctcta tcgcttgcgg aaaacgcgct
    18361 tcaggtgaaa gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg
    18421 cgcatgatgc gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa
    18481 aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat
    18541 tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg
    18601 cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt
    18661 aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca
    18721 ggtagtttgt tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac
    18781 cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct
    18841 cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa
    18901 tttcccggta aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt
    18961 cagcaattcc agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg
    19021 ctggtcgata ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc
    19081 gggcattcac tggtgattga ggatgacgta gccgaagcgc tttatcagga gctaaaacag
    19141 aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac
    19201 tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt
    19261 ggcagttatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta
    19321 caaggcgtgg atggcccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt
    19381 acctttgcta tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat
    19441 gtgattgccg gagcctcggc actggttctc acctcttatc tggtgcgttg caaaccgggt
    19501 gaacccatgc cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg
    19561 gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa
    19621 ttcctcaaag atcacgtctc tatccttgcg atgaacgaag atgaagccga agcgttgacc
    19681 ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg
    19741 tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt
    19801 aaaacccagc atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc
    19861 cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg
    19921 tacatgggcg ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca
    19981 gcgttgctgc atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc
    20041 aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac
    20101 cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag
    20161 cgtgaagaca gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc
    20221 agataaggcg ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg
    20281 cgtcttatca tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt
    20341 caccacttca cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg
    20401 ttcacccatc actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata
    20461 atgggcgcgg gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc
    20521 acccgcttca taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgcgc
    20581 cagttgcata atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg
    20641 cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag
    20701 caatttctcc cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca
    20761 aatatccact gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag
    20821 cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat
    20881 cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc
    20941 cattcccagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat
    21001 ggttaatgcc gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga
    21061 tttaccaaac agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
    21121 taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa
    21181 tggcagcgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta ccatcccggc
    21241 aaagaacgcg ccaagtgcga aggagacgtc aaacaactcc accgcaccaa aggcaatccc
    21301 taacgccagc gccagcaccg acagggtaaa tagctcgcga gaaccggttg ccgcgctgcg
    21361 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
    21421 cactttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg
    21481 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatta ccaggtcttc
    21541 cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt gccgttcttc
    21601 aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataaaccga acacgatacc
    21661 ggtcattaac gaccagccca gcacggcaga gagcgccata cccagcagcg tcgccacggc
    21721 tatctgggcg atcgcaccgg gaatggcgat ggcctttacc gccatcaaat ccttcagcga
    21781 aaagtgcaaa ccgacgccaa acatcaacag aatgacgccc agttcagcca gttccggcgc
    21841 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
    21901 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
    21961 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
    22021 tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg
    22081 acaatttcat tgatgatgtt catgaataat tattgaattt tgcagaaaaa tggaattagc
    22141 tgcaaaaaaa gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
    22201 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
    22261 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
    22321 gactaactcg atgct
//
LOCUS       DAZOYS010000055        21946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOYS010000055 DAZOYS010000000
VERSION     DAZOYS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.055"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUN9_001814"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUN9_001814"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0695228.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUN9_001815"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUN9_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0695229.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUN9_001816"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUN9_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0695230.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHUN9_001817"
     CDS             2284..2730
                     /locus_tag="ACHUN9_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0695231.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHUN9_001818"
                     /pseudo
     CDS             complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHUN9_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3604..4785
                     /gene="nimT"
                     /locus_tag="ACHUN9_001819"
     CDS             3604..4785
                     /gene="nimT"
                     /locus_tag="ACHUN9_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0695232.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4840..5187
                     /locus_tag="ACHUN9_001820"
     CDS             4840..5187
                     /locus_tag="ACHUN9_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0695233.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHUN9_001821"
     CDS             complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHUN9_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0695234.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHUN9_001822"
     CDS             5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHUN9_001822"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0695235.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6704..6802
                     /locus_tag="ACHUN9_001823"
     CDS             6704..6802
                     /locus_tag="ACHUN9_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0695236.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHUN9_001824"
     CDS             6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHUN9_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0695237.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6938..7186)
                     /locus_tag="ACHUN9_001825"
     CDS             complement(6938..7186)
                     /locus_tag="ACHUN9_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0695238.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7334..7516)
                     /locus_tag="ACHUN9_001826"
     CDS             complement(7334..7516)
                     /locus_tag="ACHUN9_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0695239.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHUN9_001827"
     CDS             complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHUN9_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0695240.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHUN9_001828"
     CDS             complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHUN9_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0695241.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHUN9_001829"
     CDS             complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHUN9_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0695242.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHUN9_001830"
     CDS             9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHUN9_001830"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0695243.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHUN9_001831"
     CDS             11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHUN9_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0695244.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHUN9_001832"
     CDS             12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHUN9_001832"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0695245.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHUN9_001833"
     CDS             14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHUN9_001833"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0695246.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHUN9_001834"
     CDS             complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHUN9_001834"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0695247.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHUN9_001835"
     CDS             complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHUN9_001835"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0695248.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHUN9_001836"
     CDS             complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHUN9_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0695249.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHUN9_001837"
     CDS             complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHUN9_001837"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0695250.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19481..21391)
                     /locus_tag="ACHUN9_001838"
     CDS             complement(19481..21391)
                     /locus_tag="ACHUN9_001838"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0695251.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21523..21867
                     /locus_tag="ACHUN9_001839"
     CDS             21523..21867
                     /locus_tag="ACHUN9_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0695252.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5365 a         5640 c         5361 g         5580 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt actatcagtc gcgcaagatt
     2941 gcgttcactc atcgcaaaaa agccagccca ttgccctaat gacccccact cgacgggccc
     3001 tttcgccatc atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag
     3061 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
     3121 cggctcagca cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa
     3181 gggcgatatt ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg gttcgataaa
     3241 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat
     3301 ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
     3361 accatgtaat gctagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
     3421 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
     3481 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
     3541 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
     3601 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
     3661 ctgatgattg ccacaacatt acgcgtcacc tttatcggcg cagcaccgtt actggatacg
     3721 attcgttccg cttactcgct gacgacagcg caaaccggct tattgaccac cctgccatta
     3781 ttggcctttg cgctaatctc acctttggcc accccggtag cgcgacgttt tggtatggaa
     3841 cgtagcctgt ttgccgcgtt acttttgatc tgtgctggta tcgcaattcg ctctctccct
     3901 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat
     3961 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
     4021 gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatgat agtgccgctg
     4081 gctttgaacg gttttggctg gcaaggcgcg ttgctcatgc tgatgtgttt tcctctgctg
     4141 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg
     4201 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt aacattgttt
     4261 cttgggatca actcactgat ctattacgtg attattggct ggcttccggc gatcctcatc
     4321 agtcacggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
     4381 gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt
     4441 gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg
     4501 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
     4561 ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg
     4621 gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctaatggg taaaatacac
     4681 gatgctaacg gtgactggtc cgtaccactg atgggtgttg caatactttc actactgatg
     4741 gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag
     4801 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
     4861 gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg
     4921 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
     4981 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
     5041 tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
     5101 aaacagccgc tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg
     5161 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
     5221 agccggatgg tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
     5281 gcaattgttg ctgacccctt gcgctgtcga cactggaatg agcgtcccgc ccttctgctg
     5341 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggta cttctggctg
     5401 agaagaggtt gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt
     5461 catttattga tctcactcat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc
     5521 agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttggtg
     5581 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
     5641 gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac
     5701 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
     5761 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
     5821 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
     5881 gcgccattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
     5941 ttgcggcacg caatcttggc atctccacat ttctgagcac accaattcac ttaccggatg
     6001 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
     6061 cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa aaagaggcac
     6121 tggttgaaca gttgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt
     6181 taaccgggtt gccgaatccg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg
     6241 cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa
     6301 tcaatgatcg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
     6361 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
     6421 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgc attcagcagc
     6481 aaatacgtgg agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg
     6541 gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata
     6601 ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg cattcagcgc
     6661 cacattgctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
     6721 ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg
     6781 ctatgctgcc ccgggggcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg
     6841 attattgtcg tactcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca
     6901 aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga
     6961 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
     7021 cgactttacc aaagccaaac agcgtgctga tccatccgcc gactacggca ccgactatcc
     7081 ccagcaggat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
     7141 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
     7201 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
     7261 agatttgtga cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa
     7321 cgatcagtga taattaccaa ccacaaacat catgttcgtt ttccgtgtca taagaacgca
     7381 cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc aaagtcatcg
     7441 gcgtctctgt ttcataatcg acagaaacgc cattactgtt attggtgacg gtcacggtat
     7501 acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt tgcgcacctt
     7561 ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg tcagtcatgg
     7621 cttcaatgtt gtgccacttt tctggaggga acacctcaaa ctgccccgct tcgataagga
     7681 tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg accgccccat
     7741 gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga tgacgttcga
     7801 atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta tgaatataat
     7861 tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa acggtttatt
     7921 ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat tggttacttt
     7981 ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg cgacaacgcg
     8041 ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac gaacatcaaa
     8101 ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta ctgcgtgaca
     8161 aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc
     8221 gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa
     8281 aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta
     8341 aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc cttcagggtc
     8401 gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata acgtacaatg
     8461 ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc cagaaacatc
     8521 aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt cataccgcta
     8581 ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac aataaaaatg
     8641 gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac attaacctct
     8701 ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct
     8761 tagaaaggag aaaatcaggt tttaacccga tatcaacccg ataattgaat aattaatcag
     8821 gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa aaattctatc
     8881 gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca gacgttcgct
     8941 ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt ttcgctaaca
     9001 tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg gacaatttct
     9061 ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc tttattgcgt
     9121 aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg caatgcggaa
     9181 agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga cgcgcagaga
     9241 atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc atcgccaatg
     9301 cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc cacatcaaaa
     9361 cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc caccacctgt
     9421 cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg acgcacgtca
     9481 gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca ttcataaatc
     9541 cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt
     9601 agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct
     9661 tccgccacgg cggcaaaacc agcccggcga gccaccagca taaagacgcg caaatcgttc
     9721 agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg ggattaattc
     9781 cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga agtgagaacc
     9841 caatgatgaa aacgatgcgt attgctgcga tcccgggaga tgggattggc aaagaagtcc
     9901 ttcctgaagg gattcgcgta ttacaggctg ccgctgagcg ctggggcttc gacttgagtt
     9961 ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa atgatgccgg
    10021 acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc gtcggctggc
    10081 cggataccgt tccggaccat atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat
    10141 tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc tgcccgctgg
    10201 cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc gaaggcgaat
    10261 attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc attcaggaat
    10321 cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa
    10381 gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc atcagcatgc
    10441 cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc cgctgggaca
    10501 agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg
    10561 tggtggcgtc caatttgttt ggcgatatcc tttccgatct tggcccggcc tgcaccggca
    10621 ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg tcgctcttcg
    10681 agcctgtcca cggttccgcg ccggatatct acgggaaaaa tattgctaac cctatcgcca
    10741 caatttgggc cggtgcgatg atgctcgatt ttctcggcaa tggcgatgag cgtttccagc
    10801 aagcgcataa cggtattctg gcagcgattg aagaagtgat tgctcacggg ccgaaaacac
    10861 cggatatgaa aggcagtgcc accacgccac aggttgccga cgcgatttgc aaaattattt
    10921 tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt tttattcgtt
    10981 ctttgcagtt aataacctgc gtcatttcac cttttattgt ttccgtttcg tgttttatgg
    11041 ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt tgttaaacac
    11101 aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag acgggctttt
    11161 atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc gctgataagc
    11221 atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc acaacaatcg
    11281 gccgacgcgg ccaacacaat atacactttt gttacgcgta cgttaggttc cgccgtacag
    11341 gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa atacggcaat
    11401 attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt tatgtttatt
    11461 tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc ctattattat
    11521 caaacgcctg gattaaatat cgcaccacgt tcacaacagg cactcgaatt tagcgtccct
    11581 tactccttct tccactgggg catcagcacc tgggcaactt atacgctggc ctcattaatc
    11641 atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg cattattgcc
    11701 gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt gatgtttctg
    11761 atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac ctttactcgt
    11821 ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt tgtgatcctg
    11881 ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg tttgcaacgt
    11941 ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct gattgtcggt
    12001 ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gcctgaccac gcaaaacttc
    12061 ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg caactggacc
    12121 gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt tgtcacccgc
    12181 gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg cagcaccgtc
    12241 ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt tatcaatggc
    12301 gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt acagcaagtt
    12361 ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt gatgattatt
    12421 ttccttgcct cgcatatgga tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat
    12481 ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact
    12541 ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc
    12601 gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg atttattcgc
    12661 tggctgaaac aggattatgc cgacattccg gctcatcaag ttgaacatta tctcccgcag
    12721 acaccggttg aagccctgga aaaaacacca gtgctccctg cgggaaccgt attcaaaggc
    12781 gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc tgagccctga
    12841 ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg
    12901 tttttatacc gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg
    12961 gatttgcgtc gctcacagca gcgaactggc gaatgccaat gattatgtga cgcgtgagat
    13021 cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg cgttctataa
    13081 cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat
    13141 tacctgccca tatcacgcat gggcattcaa actcgatggc aatctggccc atgcacgtaa
    13201 ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga
    13261 agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg tagaagatca
    13321 gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg atctgaaact
    13381 ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg ataactatct
    13441 cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac aggttgatcg
    13501 ctactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca aaccgtccga
    13561 acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct ggctgtggcc
    13621 gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt atgaattccc
    13681 ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg aagagttaac
    13741 cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg
    13801 tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat
    13861 catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt tccataatct
    13921 gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc cgctgggctg
    13981 atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag gtgagtcagg
    14041 tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccgc
    14101 tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt gataaccagt
    14161 acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat cagattgccg
    14221 ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg
    14281 gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc tcagccagaa
    14341 agcatctgtt tatcgcgggc ggtattagta tcaccccttt cctgtcgcac atggcagagt
    14401 tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca gaaagttgcg
    14461 catttcgtga tgagctagtc cagcatccgc aggctgagaa agtccatttg catcattcat
    14521 caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct ggcacacacg
    14581 tttatacctg tggccccgag gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg
    14641 acatcgccgc cgatacgctg cactttgagc aatttgctat cgaagacaaa accggcgatg
    14701 cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa gagatgacta
    14761 ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt cgtgaagggg
    14821 tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt
    14881 ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg
    14941 gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc
    15001 agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt aatgcttccg
    15061 ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt
    15121 tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga
    15181 tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat
    15241 aacccggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct
    15301 gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata
    15361 aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc gaccacaaat
    15421 gctcttcacg cacgacaaag ttcaccgcca gatcacgctc acgagccttg cgcagtcgcc
    15481 agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taattgccag gcattggtga
    15541 tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc
    15601 attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata agcttggcgg
    15661 tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg
    15721 gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt
    15781 ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg
    15841 tgtcaatcag gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac
    15901 tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc
    15961 agtcggtgat cccgagagga tcaattagcg ccagatgctc accatcgaaa agttgaatca
    16021 accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatcctgg gctatcgccg
    16081 gaaagacacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt
    16141 aattcaaatt gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg
    16201 acgactgact taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
    16261 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
    16321 tcggtacttt gtagcccgtg agctgacggc agcaaaaggt caccagtgac tcttcggtaa
    16381 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
    16441 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
    16501 tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca
    16561 ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
    16621 tctcatcggt agcatcggga cgctgccagt aacccagcat cacctgcggt cctttgacac
    16681 aaagctcacc cggttgaccc ggtggtactt cattatcgtc gtcatccacc agtttggctt
    16741 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca tatgggttaa
    16801 cgctgaccag cggcgcacac tcggtaagac catagccttc cagcagatac tgtcctgtca
    16861 gtttcaccca acgctctgcc accacttgct gcactggcat cccaccgcct gcggaaagat
    16921 gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg
    16981 tgttaacgcc agtgatagcg gtaaacggat atttcgctaa ctcttttacc aaccctggaa
    17041 tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac agcaggcagt
    17101 taatggtcag ggcaaaaatg tgatacagcg gcagtgccgt tactaccagt tctttacctg
    17161 gatgcagcaa cggcccatag gtggcgttga cctgttccag gttcgccagc atattgcggt
    17221 gagtcagcat cgcgcctttc gccacacctg tggtgccgcc ggtgtattgc aggaaggcta
    17281 aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc
    17341 tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg atgtatttaa
    17401 caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa
    17461 ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca aagttagaca
    17521 cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat
    17581 acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg
    17641 gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc
    17701 cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatta
    17761 cctcccccat attcacaaac gcaggctgat cggcgtagcg gacgaccgac tgctcaaaca
    17821 tatctatcag agattgataa cggtcagggt tgatctccgt cggaacatcc gcgggataac
    17881 ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa
    17941 ccccaagtga taaacatgtt gttaacataa cattaactca tcttaccagc ttgataatta
    18001 gacaacgaaa aggttgcgaa gcgcgtcact atttattttt atagttgccg tatgaacgca
    18061 gaaacagcgg accagccgct gtttcttttt ctttaaaaac aacttgttac tcggttacaa
    18121 ttgtttcaac tcttgcaggg ccaggattat accaacccca tccgccgtaa ccatagggcc
    18181 agccgcgtcc accataaaac catggatcaa ttggctgcgg cggcataatc acctgttgag
    18241 tgatatgcca gcgtttgtaa cctgttacct gcataaccat aaatttatat ggtgcattat
    18301 ttactttgcc atccactgtg ccagtgattg gcccgacaac cgtcaccagt tgtccacgaa
    18361 aatcgaccgg atcaagaaaa ccgttcacat cggcataaat gcgcccgcgt gaaggttctc
    18421 ccaaggttgg tcttgctccg ctgtccagcg gtacggtagc aatttccagg cgggttttcc
    18481 cttgctggtt ttgtaccaca accactttgc cgccaaagcg cgcctcctga ccaacatata
    18541 aatctggcgc attcatcacg cgtaccagat cttgctgtgg tgttggactg ctgcctttaa
    18601 tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc
    18661 ctttgataac attcttttga accgccatga tgcgactcct ttttttctca gggcatactc
    18721 ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaaccg
    18781 gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac gcaatcggca
    18841 gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc ccgctctctt
    18901 tacggagatc cggccaggct tgccagcccg tccctaccgt tacccattcg ccggaaagct
    18961 gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgcgcc
    19021 agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa
    19081 tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccctgcgcc atcgtcatta
    19141 gcgtagagac accaatcatc ggtaactccg cgccaagcgc cagcccttgc gcaataccaa
    19201 taccaatgcg cacgccagta aagctaccgg ggccgcgccc gtaagccaga gcgtttatgt
    19261 cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag
    19321 tatgttcacg agggcaaagc tcaaaatgag cgttgacatt accgtcgttc cacagagcga
    19381 cagagcagac ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga
    19441 tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg
    19501 caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga
    19561 gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaattacc agcacgccgc
    19621 gatcgtcggc gtcgcgaatc agtcgcccta ctccctgctt gagagtaatg accgcatccg
    19681 gcagctgcac ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt
    19741 ttaacagtag atcatccggc gaggtaaacg gcaatttgtc gataattacc aatgacaatg
    19801 tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga agcgcgttac
    19861 cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg
    19921 gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg catcatggca tgcgaggtac
    19981 aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgccagtt
    20041 gccgtgcaca acccggttga ttggtttgcg gcaggttgcg cggcacacag agtaacgcct
    20101 ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct atcccaagcc
    20161 gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc
    20221 taccgggctt ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag
    20281 ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct
    20341 cttttagccg cttcagccgt gtgcgataca acgtggcgcg ctcaaatgcc gcatccagca
    20401 aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg gtgtcatcga
    20461 gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat
    20521 aacctggctc accgagttgc agacgaaaat cctgtgcact ctgggcaaga cgatcagcgc
    20581 acttttgtaa ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg
    20641 ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtccg
    20701 gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc agggatcaac tcgccaaatc
    20761 cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg
    20821 cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc gggcagtcgc
    20881 tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca
    20941 cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga
    21001 tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc tgttcgagac
    21061 gttcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc gtatatttca
    21121 atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgagc
    21181 cggtcgagat aatgactttc tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt
    21241 tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc gcctgggtga
    21301 cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca
    21361 gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt
    21421 atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcaacctca cattgtggca
    21481 gtctttgcag cacgaaaatg gaagtataat gaggacaaaa tgatgactat cgttcgtatc
    21541 gatgctgaag cccgctggtc tgatgtagta atccacaaca acacgctcta ctacactggt
    21601 gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct ggcacagatt
    21661 gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc
    21721 ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg
    21781 ggtcatgcgc cagtgcgctg cacggtacaa gcgggtttga tgaacccgaa gtataaagtt
    21841 gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat
    21901 ttatcgataa tactggtcgg ttttacataa atcgaccaga gaataa
//
LOCUS       DAZOYS010000056        21643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOYS010000056 DAZOYS010000000
VERSION     DAZOYS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.056"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHUN9_001840"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHUN9_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0695253.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHUN9_001841"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHUN9_001841"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0695254.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHUN9_001842"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHUN9_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0695255.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHUN9_001843"
     CDS             complement(3717..4511)
                     /locus_tag="ACHUN9_001843"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0695256.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHUN9_001844"
     CDS             4649..4990
                     /locus_tag="ACHUN9_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0695257.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHUN9_001845"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHUN9_001845"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0695258.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHUN9_001846"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHUN9_001846"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0695259.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHUN9_001847"
     CDS             8906..10198
                     /locus_tag="ACHUN9_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0695260.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHUN9_001848"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHUN9_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHUN9_001849"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHUN9_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0695261.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHUN9_001850"
     CDS             complement(10731..11189)
                     /locus_tag="ACHUN9_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0695262.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHUN9_001851"
     CDS             complement(11186..12103)
                     /locus_tag="ACHUN9_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0695263.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHUN9_001852"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHUN9_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0695264.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHUN9_001853"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHUN9_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0695265.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHUN9_001854"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHUN9_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0695266.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHUN9_001855"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHUN9_001855"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0695267.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHUN9_001856"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHUN9_001856"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0695268.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHUN9_001857"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHUN9_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0695269.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHUN9_001858"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHUN9_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0695270.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHUN9_001859"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHUN9_001859"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0695271.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHUN9_001860"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHUN9_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5686 c         5799 g         5144 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtagcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZOYS010000057        21005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOYS010000057 DAZOYS010000000
VERSION     DAZOYS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.057"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHUN9_001861"
     CDS             complement(<1..172)
                     /locus_tag="ACHUN9_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0695272.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUN9_001862"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="ACHUN9_001862"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0695273.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="ACHUN9_001863"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="ACHUN9_001863"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0695274.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUN9_001864"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="ACHUN9_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0695275.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUN9_001865"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHUN9_001865"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0695276.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUN9_001866"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHUN9_001866"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0695277.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="ACHUN9_001867"
     CDS             4290..5153
                     /locus_tag="ACHUN9_001867"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0695278.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUN9_001868"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="ACHUN9_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0695279.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="ACHUN9_001869"
     CDS             6491..7108
                     /locus_tag="ACHUN9_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0695280.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUN9_001870"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHUN9_001870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0695281.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUN9_001871"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="ACHUN9_001871"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0695282.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUN9_001872"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHUN9_001872"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0695283.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUN9_001873"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="ACHUN9_001873"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0695284.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUN9_001874"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="ACHUN9_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0695285.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="ACHUN9_001875"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="ACHUN9_001875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0695286.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUN9_001876"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHUN9_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0695287.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUN9_001877"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="ACHUN9_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0695288.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="ACHUN9_001878"
     CDS             18074..19783
                     /locus_tag="ACHUN9_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0695289.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUN9_001879"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHUN9_001879"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0695290.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5084 a         5718 c         5386 g         4817 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 acactacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaac ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg catgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca ccaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAZOYS010000058        20820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOYS010000058 DAZOYS010000000
VERSION     DAZOYS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.058"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHUN9_001880"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHUN9_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0695291.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="ACHUN9_001881"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="ACHUN9_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHUN9_001882"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHUN9_001882"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0695292.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHUN9_001883"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHUN9_001883"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0695293.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHUN9_001884"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHUN9_001884"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0695294.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHUN9_001885"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHUN9_001885"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0695295.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHUN9_001886"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHUN9_001886"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0695296.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="ACHUN9_001887"
     CDS             8329..10047
                     /locus_tag="ACHUN9_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0695297.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHUN9_001888"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHUN9_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0695298.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHUN9_001889"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHUN9_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0695299.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="ACHUN9_001890"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="ACHUN9_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0695300.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHUN9_001891"
     CDS             complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHUN9_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0695301.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHUN9_001892"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHUN9_001892"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0695302.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHUN9_001893"
                     /pseudo
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHUN9_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHUN9_001894"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHUN9_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0695303.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..20423)
                     /gene="mdtN"
                     /locus_tag="ACHUN9_001895"
     CDS             complement(19392..20423)
                     /gene="mdtN"
                     /locus_tag="ACHUN9_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0695304.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20442..20717)
                     /locus_tag="ACHUN9_001896"
     CDS             complement(20442..20717)
                     /locus_tag="ACHUN9_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0695305.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4690 a         5516 c         5697 g         4917 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggtcagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggcccggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gctatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct gggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaactgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggtc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctacgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca ttgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagga
    20401 gcttttttcg gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag
    20461 aacagcatgg cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg
    20521 atatttgccc gctgaataac tcgcctcgtg atgagcatca aaataaggct ggcaatggcg
    20581 cagaaaaacc agctgggata ataagcaccg atcaccggga ttgccggaga taatgagcat
    20641 cccgaaacca tgaagacggg aattatccag gctgtttttt tgagttgcat cgccatgcga
    20701 gagagaactg ttggcatatt aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat
    20761 atcaatatcc attgaaggcg tcagtatggc tattttctcg atgaataaaa atagctttat
//
LOCUS       DAZOYS010000059        20706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOYS010000059 DAZOYS010000000
VERSION     DAZOYS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.059"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHUN9_001897"
     CDS             <1..2064
                     /locus_tag="ACHUN9_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0695306.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHUN9_001898"
     CDS             2080..3099
                     /locus_tag="ACHUN9_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0695307.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUN9_001899"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUN9_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0695308.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUN9_001900"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUN9_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0695309.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUN9_001901"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUN9_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR0695310.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUN9_001902"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUN9_001902"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0695311.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUN9_001903"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUN9_001903"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0695312.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7552
                     /locus_tag="ACHUN9_001904"
                     /pseudo
     CDS             6828..7552
                     /locus_tag="ACHUN9_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            7604..8350
                     /locus_tag="ACHUN9_001905"
     CDS             7604..8350
                     /locus_tag="ACHUN9_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0695313.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8324..9289)
                     /locus_tag="ACHUN9_001906"
     CDS             complement(8324..9289)
                     /locus_tag="ACHUN9_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0695314.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9286..10290)
                     /locus_tag="ACHUN9_001907"
     CDS             complement(9286..10290)
                     /locus_tag="ACHUN9_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0695315.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10287..11564)
                     /locus_tag="ACHUN9_001908"
     CDS             complement(10287..11564)
                     /locus_tag="ACHUN9_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0695316.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11821..12873
                     /gene="fbaB"
                     /locus_tag="ACHUN9_001909"
     CDS             11821..12873
                     /gene="fbaB"
                     /locus_tag="ACHUN9_001909"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0695317.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13192..14046
                     /gene="gatY"
                     /locus_tag="ACHUN9_001910"
     CDS             13192..14046
                     /gene="gatY"
                     /locus_tag="ACHUN9_001910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0695318.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14075..15332
                     /gene="gatZ"
                     /locus_tag="ACHUN9_001911"
                     /pseudo
     CDS             14075..15332
                     /gene="gatZ"
                     /locus_tag="ACHUN9_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15342..15794
                     /gene="gatA"
                     /locus_tag="ACHUN9_001912"
     CDS             15342..15794
                     /gene="gatA"
                     /locus_tag="ACHUN9_001912"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0695319.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15825..16109
                     /gene="gatB"
                     /locus_tag="ACHUN9_001913"
     CDS             15825..16109
                     /gene="gatB"
                     /locus_tag="ACHUN9_001913"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0695320.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16113..17468
                     /locus_tag="ACHUN9_001914"
     CDS             16113..17468
                     /locus_tag="ACHUN9_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0695321.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17516..18556
                     /gene="gatD"
                     /locus_tag="ACHUN9_001915"
     CDS             17516..18556
                     /gene="gatD"
                     /locus_tag="ACHUN9_001915"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0695322.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18662..19435
                     /locus_tag="ACHUN9_001916"
     CDS             18662..19435
                     /locus_tag="ACHUN9_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0695323.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19517..20416)
                     /gene="yegS"
                     /locus_tag="ACHUN9_001917"
     CDS             complement(19517..20416)
                     /gene="yegS"
                     /locus_tag="ACHUN9_001917"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0695324.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         5371 a         4858 c         5302 g         5175 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttctaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 tgcctttatt aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
     7081 gcaactaagc cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt
     7141 agcagcaccg gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt
     7201 ccctgcggta ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag
     7261 ggtaagtcag tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc
     7321 aggagccgtt ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt
     7381 ggctcactac tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
     7441 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
     7501 cacggtggag gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag
     7561 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
     7621 ccagctgatt gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat
     7681 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
     7741 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
     7801 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
     7861 gatcaacaac atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt
     7921 gttgcgcggg aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga
     7981 agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat
     8041 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
     8101 tcatgatgtt gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta
     8161 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
     8221 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
     8281 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
     8341 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
     8401 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
     8461 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
     8521 atgccggaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
     8581 gaacgatcaa cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg
     8641 cggataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag
     8701 gtcgacaggc catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa
     8761 aatcgataaa cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc
     8821 agggcgaggc cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc
     8881 gcgccagcca ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt
     8941 cgccatccgg ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca
     9001 ggctgattaa gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac
     9061 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
     9121 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
     9181 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
     9241 gacgcgtggt taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg
     9301 ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
     9361 atccagttcc gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat
     9421 tgccgtcgcc atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc
     9481 gcagcgattc gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc
     9541 aatagtgctg gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
     9601 gtcggcattg cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa
     9661 cgtagtgatg cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc
     9721 tgaccagctt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
     9781 aaccgccaga tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga
     9841 atcaacatca cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
     9901 ggtcacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
     9961 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc
    10021 gagaatatta cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc
    10081 cgccagacac agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc
    10141 cgcgttattc tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac
    10201 gcggctgcgt ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata
    10261 aagagcaccg agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg
    10321 cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac
    10381 agcacggcga taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa
    10441 gtcagtccgt ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga
    10501 cgatagccga gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg
    10561 gtacgcatat gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa
    10621 tcgtaactta cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca
    10681 tccgtactac cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat
    10741 aatacctttt tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt
    10801 tcagagaact ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga
    10861 taaccgttgg caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag
    10921 aaaaagacga ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact
    10981 ttaatatcca ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca
    11041 ccgagcagag cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg
    11101 gcatacccca gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata
    11161 gtgcccatca cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg
    11221 atgctgttag tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga
    11281 aagaacccgg caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac
    11341 atcaataccg ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga
    11401 attggcgaca ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca
    11461 ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac
    11521 cattcaacaa acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat
    11581 gacaggtaat gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt
    11641 gcggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct
    11701 gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc
    11761 aaaaggcggg ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta
    11821 atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt
    11881 atgacaattc cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg
    11941 attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg
    12001 cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct
    12061 gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg
    12121 gcgatcgaag cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg
    12181 cggcgctatg cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt
    12241 tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc
    12301 gcggtggcgg ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa
    12361 gaaatttctg cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc
    12421 tatttgcgaa actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg
    12481 accggtcagg caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg
    12541 gcggaaaata acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat
    12601 agcaaattga ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat
    12661 atgggccggg ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt
    12721 gatgcagtgc gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga
    12781 cgtaaagcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac
    12841 gtttatctcg atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc
    12901 gtaggacgga taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag
    12961 cgtcttatca tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc
    13021 cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct
    13081 ttcactcctc gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa
    13141 caaatcattt cattatattt tgaaatcgaa aacaagcgac aggatatgaa aatgtacgtg
    13201 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
    13261 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
    13321 ccggtcatca tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg
    13381 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
    13441 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
    13501 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    13561 tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat
    13621 gatgtgcaag tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt
    13681 gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat
    13741 gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta
    13801 ccgctggtgc tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa
    13861 ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta
    13921 aaaaattacc tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct
    13981 aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg
    14041 gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa
    14101 agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc
    14161 ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca
    14221 ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt
    14281 tacgattgcc gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct
    14341 ggggccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct
    14401 ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg
    14461 cgcgggggat cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg
    14521 ctgcggaaag tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg
    14581 aagttccggt tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg
    14641 aagatgccgc caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag
    14701 aggcgttaac acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca
    14761 atattatcca ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc
    14821 gaatggttta tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag
    14881 tccgcgatca ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg
    14941 cgatatttgc actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt
    15001 gcctggcggt aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc
    15061 gtacgggttt taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt
    15121 attactggcc gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag
    15181 gcgtggacat cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc
    15241 agtccgggga attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt
    15301 tgcgcgccta tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc
    15361 gtagcggaat ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc
    15421 tcgccaaagg tgtggttcat gatacctggc cacaggcatt aattgccaga gaagcagaat
    15481 tccctaccgg gataatgctt gagcagcacg ccattgcaat accgcattgt gaggcgattc
    15541 atgctaagtc gtcagccatt tatctgttaa ggccaacaaa taaagttcat tttcagcaag
    15601 cggatgatga taacgacgtg gaggtatcgt tggttattgc gttgattgtg gagaatccgc
    15661 agcagcaatt gaaactttta cgctgtttat ttggcaagtt acaacagccc gatatcgtcg
    15721 agacactaat cactcttcct gaaacccagt taaaggaata cttcacaaag tatgttttag
    15781 attcagacga ataaatccct ctgtaacaat aaaaaggact atttatgaaa cgcaagatta
    15841 ttgtcgcttg cggaggcgcg gttgcgacct ctacgatggc ggcggaagaa attaaagagt
    15901 tgtgtcagaa tcatagtatt cctgttgaat taatccagtg tcgggttaat gaaatagaaa
    15961 cctatatgga tggtgtgcat ttgatatgca ccactgccaa agtggatcgt agttttggcg
    16021 atattccgtt agttcacggc atgcctttta tttctggtgt cggtatcgaa gcattacaaa
    16081 ataaaattct gactatctta caggggtgac ctatgttttc agaagtcatg cgttatattc
    16141 tcgacctcgg ccctacggtg atgcttccga ttgtcatcat tattttttct aaaatattag
    16201 gcatgaaggc aggcgattgc tttaaagcgg gtctgcatat cgggattggc tttgttggca
    16261 ttggccttgt gattggctta atgctggatt ccattggtcc ggcggcgaaa gcgatggcgg
    16321 aaaatttcga cctgaatctg catgtggtcg atgtcggctg gccgggctct tcaccaatga
    16381 cctgggcgtc gcaaattgcg ctggtggcga ttccgattgc gattctggtt aacgtggcga
    16441 tgttactgac ccgtatgacg cgggtggtaa atgttgatat ctggaatatc tggcatatga
    16501 ccttcaccgg cgcgttgctg catctggcaa ccggttcatg gatgataggg atggcaggtg
    16561 tggtaattca cgcggcgttt gtttataagc tcggcgactg gtttgcccgc gatacccgaa
    16621 atttctttga gctggaaggt attgctattc cgcacggtac gtcggcgtat atggggccga
    16681 ttgcggtgct ggtcgatgct atcatcgaga aaatcccagg cgttaaccga attaaattta
    16741 gcgccgacga tattcagcgc aaatttggtc catttggcga gcctgtcacc gtgggttttg
    16801 tgatggggct gattatcggc atcctcgcgg gttacgatgt caaaggtgta ttgcagctgg
    16861 cggtaaaaac ggcggcagtt atgctgctga tgccacgggt gattaaaccc atcatggatg
    16921 gtttaacgcc catcgctaag caggcccgta gtcgtttaca ggcgaagttc ggcggtcagg
    16981 agttcctgat tggcctggat cccgcattac tgctggggca tacggcggtg gtatcggcaa
    17041 gcctgatttt tatcccgctc accattttaa ttgctgtttg tgttccgggt aatcaggtac
    17101 tgccgtttgg cgatcttgcc accatcggct tcttcgtggc gatggcggtc gcggtgcatc
    17161 gtggaaatct gttccgcacc ttaatctcgg gtgtcatcat tatgagcatc accctgtgga
    17221 tcgcgacgca aactattggt ttgcacaccc aactggcggc taatgctggg gcgttaaaag
    17281 ccgggggtat ggtggcttca atggatcagg gcggttctcc cattacctgg ttactgattc
    17341 aggttttctc cacgcaaaat attcccggtt tcattattat cggcgcaatt tatcttaccg
    17401 gtattttcat gacctggcgt agagcgcgtg gttttattaa acaagagaaa accgttctcg
    17461 cagaataatt tttacctgag ggggaggtta tcccccttaa caatcaggag tttttatgaa
    17521 atcaatggtg aatgatactg atggtatcgt gcgcgttgca gaaagcgtca ttcctgaaat
    17581 taaacatcag gatgaggtgc gggtaaaaat tgccagctcg ggcttatgtg gttccgattt
    17641 acccaggata tttaaaaatg gtgcacatta ttatccaata acgttaggcc atgaatttag
    17701 cggctatatt gatgcggtgg gatccggtgt tgatgattta caccctggcg atgcggttgc
    17761 ctgtgtgccg ttattaccct gttttactta tccagagtgt ttgaaagggt tttattccca
    17821 gtgcgcaaaa tatgatttta ttggctcgcg gcgtgatggt ggatttgctg aatatattgt
    17881 cgttaagcga aaaaatgtct ttgctctacc cacggatatg cctattgagg atggggcttt
    17941 tattgagccg attaccgttg gcctgcatgc ttttcattta gcgcaaggtt gtgagaataa
    18001 aaacgttatt attattggtg ccggaaccat tggcctgctg gctattcagt gcgctgtcgc
    18061 gctgggagca aagagtgtga cggcgatcga cattagctca gaaaaactgg cattggcaaa
    18121 atctttcggt gcgatgcaaa catttaacag tagcgaaatg agcgcgccgc aaatgcagag
    18181 cgttttacgc gaactgcgct ttaatcagct tatcctcgag acggctggcg taccgcaaac
    18241 tgtcgaactg gcggtagaga ttgccggtcc tcatgcccaa ctggcgctgg tgggcacgtt
    18301 gcatcaggat ctgcatttaa catcggcaac gtttggcaaa atattgcgta aagagctgac
    18361 ggttatcggc agttggatga actattccag cccttggccg gggcaggagt gggaaacggc
    18421 gagccggttg ctgacagaac gtaagttaag cctggagcca ttaatcgctc accgtggaag
    18481 ctttgaaagc ttcacccagg cggtgcgtga catcgctcgt aatgccatgc cgggcaaagt
    18541 gttgctcatt ccatgaaacc gcgggccagc gtgatgctgg cccggtattg tgcaaaacag
    18601 atcattcacc aatggtcccc cttcgtttac actagccaca attgaatatg ggtaaatgac
    18661 gatgaattca ttcgagcgaa ggaataagat catccaatta gtgaatgaac agggaaccgt
    18721 gcttgttcag gatctggcgg gagtatttgc tgcctcggaa gcgacaatcc gtgccgattt
    18781 gcgctttctc gaacaaaaag gcgtggttac gcgctttcat ggcggtgcgg cgaaaataat
    18841 gtccggtaat agtgaaaccg agacccagga agtcgggttt aaagagcgat ttcagctcgc
    18901 cagcgcgcca aaaaacagaa tagcgcaggc ggcagtcaaa atgatccatg aagggatgac
    18961 cgttattctc gacagcggaa gtacgacaat gcttatcgcc gaaggattaa tgaccgctaa
    19021 aaatatcacg gtgattacca acagtctccc ggcagcgttt gccctttccg aaaataaaga
    19081 cattactctg gtcgtctgtg gtggcacggt ccgccataaa acgcgctcga tgcatggttc
    19141 cattgccgag cgttcattgc aggatattaa tgccgattta atgtttgtcg gtgctgatgg
    19201 tattgatgcg gttaatggta ttacgacctt taatgaaggt tattcgatta gcggtgcgat
    19261 ggtcaccgcc gctaataaag tgattgccgt tctcgattca tcgaaattta accgtcgcgg
    19321 ttttaatcag gtactgccaa tcgaaaaaat tgacattatt attaccgatg atgctgtatc
    19381 agaggtggat aaactggcat tgcagaagac acgggtaaaa ttaattacgg tgtagtattg
    19441 ttctgtatgc cggataaggt atttacaccg catccggcat ttcggattct ccctggttca
    19501 agagagaata gtttgattaa cgcaataata gacaatctgg tggtaatcga caacgcaacg
    19561 ccgccggaag tatttcaata tgaaagtttt gtccgctcaa cggttcgcca tcaagattaa
    19621 aagtgatttc gtgtggggct tgtatatcaa accacgacga agcgccttcg ataatattcg
    19681 ggttatcttc gtcagatttt aaggttgata cgagagccgg aagaatttca tcgccggtaa
    19741 aaatgcgcag ttgcagcaag ccatcgttaa ttaacgcgtt cggacacaat tgctgaccgc
    19801 caccggcctg acgcccgtta ccaataccaa tgaccagggc gtcaccttgc cagtgaaagt
    19861 tttcaccgcg gatttcacaa cggtccggtt gcagagtatc catgcgcatt aagccatgaa
    19921 tgatgtaaga gacgccaccc agcgcggctt ttaatttttc cggcgtttct gtggtaatac
    19981 gcgtcccaaa tccgcctgtc gccatattaa taaaacaggt ttgtttgttg acctgcgcca
    20041 tatctatcgc aatggcgtta ccggcaattg ccagtttcag cgccttatcc agtgcctcag
    20101 gaatccctac actggtggca aaatcattgg cggttcctaa tggcaaaatt cccagcgcgg
    20161 gtatgccatc cccctcacac tgaatcaacg ccgtagaaac ttcattaatg gtgccatcgc
    20221 caccaccggc aatcaccgtt gcgacgccca acttccgggc ctcctctaca tatcgtgcgg
    20281 catcgccttt ctcccaggtg acccgcacat ggatcgtcat tccttcctca cgcaacagca
    20341 taattgcttc gcgcaagggt agattgtcag tacttttgcc attaagaatc agtaagctgg
    20401 cgggaaattc tgccatgatc gtatgtgcct ttatgattgg tctgtagata gtgtagagca
    20461 gaaaacaaaa ggtggagtca gaacaggatg aaagtcggag gataaaaagt cagttcaagt
    20521 atggaggatt cagtggttac caccaggagc ttgcaatggg gacagcgaat actaacggct
    20581 gccaccgata aatttcaaaa aagagcatat acctaatatt caactaaaca gtggcatctt
    20641 caatataata tattaaagcc cccatggagt taccctgaag ggcctcaatg tccgtaattc
    20701 ctactt
//
LOCUS       DAZOYS010000060        20369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOYS010000060 DAZOYS010000000
VERSION     DAZOYS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.060"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            287..787
                     /gene="tssB"
                     /locus_tag="ACHUN9_001918"
     CDS             287..787
                     /gene="tssB"
                     /locus_tag="ACHUN9_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0695325.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            822..1046
                     /locus_tag="ACHUN9_001919"
     CDS             822..1046
                     /locus_tag="ACHUN9_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695326.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1097..2572
                     /gene="tssC"
                     /locus_tag="ACHUN9_001920"
                     /pseudo
     CDS             1097..2572
                     /gene="tssC"
                     /locus_tag="ACHUN9_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2579..2992
                     /gene="tssE"
                     /locus_tag="ACHUN9_001921"
     CDS             2579..2992
                     /gene="tssE"
                     /locus_tag="ACHUN9_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0695327.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2996..4846
                     /gene="tssF"
                     /locus_tag="ACHUN9_001922"
     CDS             2996..4846
                     /gene="tssF"
                     /locus_tag="ACHUN9_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0695328.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4810..5892
                     /gene="tssG"
                     /locus_tag="ACHUN9_001923"
     CDS             4810..5892
                     /gene="tssG"
                     /locus_tag="ACHUN9_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0695329.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5917..7197
                     /gene="tagH"
                     /locus_tag="ACHUN9_001924"
     CDS             5917..7197
                     /gene="tagH"
                     /locus_tag="ACHUN9_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0695330.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7194..7718
                     /gene="tssJ"
                     /locus_tag="ACHUN9_001925"
     CDS             7194..7718
                     /gene="tssJ"
                     /locus_tag="ACHUN9_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0695331.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7721..9052
                     /gene="tssK"
                     /locus_tag="ACHUN9_001926"
     CDS             7721..9052
                     /gene="tssK"
                     /locus_tag="ACHUN9_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR0695332.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9057..9818
                     /gene="icmH"
                     /locus_tag="ACHUN9_001927"
     CDS             9057..9818
                     /gene="icmH"
                     /locus_tag="ACHUN9_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0695333.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9827..12622
                     /gene="tssH"
                     /locus_tag="ACHUN9_001928"
                     /gene_synonym="clpV"
     CDS             9827..12622
                     /gene="tssH"
                     /locus_tag="ACHUN9_001928"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR0695334.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESEQDNTGAEPADEADREQPEETAETVSPVQ
                     RLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWL
                     GDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYG
                     GRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKA
                     HPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLY
                     PVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVT
                     DEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTAD
                     VEDAQNDESVTKDETVL"
     gene            12619..13362
                     /gene="vasI"
                     /locus_tag="ACHUN9_001929"
     CDS             12619..13362
                     /gene="vasI"
                     /locus_tag="ACHUN9_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0695335.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13367..14779
                     /gene="tssA"
                     /locus_tag="ACHUN9_001930"
     CDS             13367..14779
                     /gene="tssA"
                     /locus_tag="ACHUN9_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0695336.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14888..18322
                     /gene="tssM"
                     /locus_tag="ACHUN9_001931"
                     /pseudo
     CDS             14888..18322
                     /gene="tssM"
                     /locus_tag="ACHUN9_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18333..19782
                     /locus_tag="ACHUN9_001932"
                     /pseudo
     CDS             18333..19782
                     /locus_tag="ACHUN9_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19709..20191
                     /locus_tag="ACHUN9_001933"
     CDS             19709..20191
                     /locus_tag="ACHUN9_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0695337.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         4824 a         5566 c         5615 g         4364 t
ORIGIN      
        1 tatatacttc aataagttac acaaaaactc tatatttaaa ttattgccat aatacacctg
       61 acgcgaattg cacaggttca cacaatctat ttaacgcttg cagaataccc ctctttacga
      121 tcgataaaaa gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt
      181 ggtgatttta ttttcaccgc cattagggcg gcattgccga tatttttttg cggtacaaat
      241 atgccgtgtt catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt
      301 tgaaggcagc gtcgcgcccc gcgagcgtat taatatatcg tatgtcccaa aaacagatgg
      361 ccagacggct gaagtggaac ttccgcttaa catgctcgtt gtcggcgata ccggaaatac
      421 gcaggaaaca tccccactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg
      481 tgcagtgatg gctgaagcag ccattggcct caattttact gtccccgcca cgctgaaggg
      541 aagcacaaca gacgatgaac tgaatgtggc gctgaatatc aaatcactgg atgatttttc
      601 accagatagc gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc
      661 actgactgca ctgaaggggc cgatgggtaa ccttccggca ttccgtaccc agctccaggc
      721 attgctggaa aatgaagagt cccgcgagca gttgctcaaa gaaatcggtc tggtgagcaa
      781 taaataacca ccactatttc attaaacagg gttatccagg catgacggca cacaaaaata
      841 tctccgggac ataccacctc tctgttgcag acattctgca ggtggtttac caggtatgtt
      901 tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc gctgattaca acactggatc
      961 gccgtatcag tgatctattg gatgagatta tccatttttg tgagtttcag cagagcgcgt
     1021 cgcactggca gcgcgtactt cactgatggc gaaaagccgg tttcagaaac acagctaaaa
     1081 cagaaggatt ttcagtatgt ctttacagga agaggaactc gtttccagtc atgccgggca
     1141 gcctgagcag gcatcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcccgg
     1201 ttcggaaggt tatgacgttg ccctccaggg tgtgacagcc tttattgcca gcatcctgca
     1261 atccactgca tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat
     1321 tgatgaacgc atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt
     1381 cgagtcgttc tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat
     1441 caaggtcaac gtgcttcatg tcaccaagca ggaacttctg gaagactttg agtttgcgcc
     1501 agaaattatt cagtccggat tttataagca cgtttattcg tccggtttcg gacagtttgg
     1561 cggtaaaccc atcgctgccg tgctgggcgc ttatgagttc aaaaataccg cgcctgacat
     1621 gaaactgctg caatacgtca gtgccgtggg cgcaatggcg cacgcaccgt tcctgtcctc
     1681 cgtttccccg gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct
     1741 ttatgccatc tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga
     1801 ttcgcgctat ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac
     1861 agataaccct gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta
     1921 tctgtggggt aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata
     1981 ccgctgatgt ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccgat
     2041 gcatctgttc gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac
     2101 cgatcgccgc gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga
     2161 ctctgataat gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg
     2221 aaaagatgca gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa
     2281 tcggttagcg cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag
     2341 gagcgactta gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa
     2401 cccgcctgca gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga
     2461 tgtagaaggc gaacccggct ggtaccaggt tgcgctaagc gtgagacctc attttaaatt
     2521 tatgggggca aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat
     2581 gataccggat cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcggc
     2641 cagaaaccgg cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctacg
     2701 aaacattctt aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga
     2761 accggaaggg gctgaaaact tccgcgaatc catgagtcgg gccatcgagc aatgtataga
     2821 acgctatgaa ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag
     2881 cccgctggat atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt
     2941 gcttgaattt gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga
     3001 atttgaagaa cgctatttcc gggaagaact cgattacctg cgccagctta gcaagctgct
     3061 ggcaacggaa aaaccccatc tggcccgctt cctggccgaa aaagatgcag atccggatat
     3121 tgaacgcctg ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga
     3181 tgaattccca gaactgacgc acgggcttat taagatgcta tggcctaatt acctgcgtcc
     3241 ggttccggca atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt
     3301 gttaatcccc cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt
     3361 gctgccctct gatgctaaaa aggaggagcc gcctccctgt accttcaccc tctgccggga
     3421 tatctggctg ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg
     3481 tgttatcaac atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa
     3541 caaacttcgc ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg
     3601 gttctgcgaa tacttgcagg gtgccgacct gactgtgggt gaacagcata ttcgcctgcc
     3661 tgagtttatg ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa
     3721 aaacgtccac agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct
     3781 cttttttgat ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac
     3841 cctgcaactg cgtttttcgc gccctttgcc cgtggacatc cggctgcgcc gcgattccct
     3901 gcgcctgtat tgcgcacctg ccattaattt atttatccac catgcagaag ccatcacgct
     3961 ggacaaccgg cgggcagact atccgctggt tcccagccgc cattacccac aacattacga
     4021 tgtattttcc gttaacagtg tggtgagcca ggtccaggat atgttcagga aaaaagatct
     4081 ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca gcctttgaaa gtttcagcca
     4141 tcagatagaa tacagccgga agcgggaagt ggtgtactgg catcaccgga ccaaaacatc
     4201 cctgttccat cgcggctttg atcataccct tgcctttata catgctgatg gcagttatcc
     4261 gtcagacgaa tctctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg
     4321 tgagcttccg tcacaaattc gttccggcga tatcaccggc acaaccggaa aaaatgcggc
     4381 tgttgcttca tttcgcaaca ttacccgccc gacgcaacca ctctggccgg tcattgatgg
     4441 cagcctgcac tggcccctac tctccgccat gaacctgaat tatctgtcat tactggatac
     4501 ggacgcgctg aagcaggtca tcgccaactt tgatcgccac gcaatccatc atccgcagac
     4561 ggcacggctg tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccccgttga
     4621 tcgcctgttt acgggtattc ccgtccgggg gctggcttcc acgctgtatc tgcacccgga
     4681 gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc
     4741 gctgtacgcc agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga
     4801 gacatggaaa tggacggaaa gaatcgggca gcatcctctt atctgagtcc gggaaatccc
     4861 ccggcagata aagagcagaa tgatccgctg gcacaggttt tccataatgc ctgctcttac
     4921 aatttttttg cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca
     4981 gaattatccc tgcgcgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc
     5041 cttgccttcc cgtgcagtga tatcagtgcg ctgaaacggg atacgtcagg ggcattcagg
     5101 atgaccacca cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc
     5161 gctcacctgg cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg
     5221 ttcagccacc gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc
     5281 agttttcgta acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg
     5341 ggccaccgcc agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc
     5401 gggatcctgg caaatccggg acgatccccg gaaattatct gtggcctggt gtcccactgc
     5461 tttgatttgt cagaagtgac actgcaaaac tggcaacgtc gcaaggtgga tattgaaccg
     5521 gatcagcaaa atagtctggg gagctattct cttaaaaatg gcgaaaaact ggcgggaaga
     5581 tcggtactgg gcaactttgt tctgggtaca cgagtgcccg atctcagcgg aaaattccag
     5641 ctcagtatta ccagcctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac
     5701 ttcctgccac tgacgatgtt cgtgtccttc atcctgcgcg atcagcttgc ctgggactta
     5761 catctggggc tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg
     5821 ctgctgggct ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg
     5881 gtacggtcgt aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg
     5941 ctttcattgc aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtctg
     6001 ttcacacagc agggaaatgt cggccatggc cccgaatgcc actggtcggt acaggatcgt
     6061 cagcagagca ttccggccca ggcttttacc gttatcctgc acgatggcac attttgtctg
     6121 cgcccgcaga cggcacaact gtggctgaat caggcaaaag tcacagcaac atcagaccta
     6181 atacagttgc gccagggcga tgagatccag atcggacggc tgatggtgag ggttcatctg
     6241 aaccggggag atattcccca ttacgatgag gaaatggcca ctcccgaaac catcgttacc
     6301 aatcgcgata tgctcacgga taccctgcta tcaacggagg gtgcgccaca ctatccggga
     6361 atgactcacc ggcaccagct tgcagacacc gtggtaaacg gtttttctgc cgatccactc
     6421 caggctcttc agtccgaaag cctgattacc acgggcgatc cgctttcagg cattgcggct
     6481 gtccggccat cggcaccgct gtccgatccg gcaagtaatg gggggatcaa tactccgttt
     6541 atggatctgc cgcccattta tgccagcccc ggcgatcgta atgacgacgt ctctgcggca
     6601 gaaatggcgc aacgccacgt tgcggtcacc cccttactgc gcggtcttgg cggctcgctt
     6661 accatgagca attccgacga tgcggatgat tttctggagg aggccggacg aacgttacag
     6721 gccgcaataa aaggtctgct cgatttgcag cagcagcgta acagcctctc agacaaacat
     6781 ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac
     6841 gtaatgtttg ccgaaggtaa aagcccggta catctggcgg ctcccgccgc cgtcagtgaa
     6901 agcctgcgca atgtccgcca ccacgaagaa gctaaccggg cagcgattgt ggagtcgctt
     6961 cgtgtcctgc tggatgcttt ctcaccacaa aatctgctgc gccgctttgt gcagtaccgc
     7021 cgcagccatg aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat
     7081 tattacgaag aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc
     7141 tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca
     7201 ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga
     7261 aaaaaatcag tgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggagcaac
     7321 cttcggaaat aaccgtcacc ctgctgaccg aaccggacac caataccaat gcggaaggtg
     7381 aatcagccgc cgtggatgtg caactggtgt acctgactga cgactcaaaa ttgctggcag
     7441 ccgactatga ccagatagcc agcacgccgc taccggatgt gctgggtaaa aactatatcg
     7501 accaccagga tttcaacctg ttgccggaca ccatcaaaac cctgccgccg gtaaagctgg
     7561 atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc cgatgaccag gccactgaat
     7621 ggaaacaaat cgagacggtg gaaggaatcg gccaccatta ccgcctgctg gtgcatgtac
     7681 gtcaaagcag catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa
     7741 ggtgatgtgg caggaaggga tgctgatgcg tccacaccat ttccagcagc agcagcgtta
     7801 caacgactac ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac
     7861 cgaactcacc ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc
     7921 aggcacactg cccgacggca ccgtcttttc tatccccgac caggacgcac tgcccgatcc
     7981 gctgcacccg caaaattttc cggacgaaag aagccgcaat atctacctcg ctctgccggt
     8041 cgccagtgat gtgagaaatg aaatcagcga cggacggcga atcgggcgtt accggctgaa
     8101 ttatgccgat gtccgggatt tgcattcaga agaaggtgac gcgcgaacat tgacgctggg
     8161 tcaactgacg ccgcgcatta tgagcggtgc agaagatatg agcgcctata ttacgctccc
     8221 gctttgccgt atcagcgatc gccatgccga tggctccctg acgttggatg acgattttat
     8281 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     8341 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     8401 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     8461 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     8521 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
     8581 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
     8641 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
     8701 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
     8761 cgtggcggca aatatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
     8821 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atcccctgta ttccattgcg
     8881 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
     8941 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
     9001 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
     9061 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
     9121 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
     9181 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
     9241 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
     9301 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
     9361 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
     9421 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
     9481 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
     9541 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
     9601 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
     9661 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
     9721 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
     9781 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
     9841 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
     9901 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
     9961 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    10021 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    10081 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctggctgc tggcgtcggc
    10141 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    10201 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    10261 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    10321 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    10381 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    10441 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    10501 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    10561 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    10621 ggccggggcg tcggtgaaag gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    10681 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    10741 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    10801 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    10861 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    10921 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    10981 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    11041 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    11101 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    11161 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    11221 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    11281 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    11341 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    11401 agagtcagag tcagagtcag agtcagagtc agagtcagag caggataata ccggtgccga
    11461 accggctgat gaggccgaca gagaacaacc ggaagagacc gctgaaacag tttccccggt
    11521 acagcggctg gcccagctca ctgccgaact ggacgccctg cataacgacc ggttgctggt
    11581 ctccccgcac gtcgataaaa aacagattgc ggcggtgatt gccgaatgga ccggcgtgcc
    11641 gctcaaccgc ctgtcgcaga atgaaatgtc ggtcatcacc gacctgacga aatggctggg
    11701 tgacaccatc aaaggccagg acctggcgat tgccagcctg cataagcatc tactgaccgc
    11761 acgcgccgac ctgcgtcgtc cgggacgccc gctcggtgcg ttcctgctgg ctggccccag
    11821 cggtgtgggt aaaaccgaaa ccgtcctgca actggcagag ctgctctacg gcggtcgcca
    11881 gtacctgacc accatcaata tgtccgaatt ccaggagaaa cacaccgtct cgcggctgat
    11941 tggttcgcct ccgggctacg ttggctacgg tgaaggcggc gtactgaccg aagcgattcg
    12001 ccagaaaccc tactcggtag tactgctcga tgaagtggaa aaagcgcacc cggatgtgct
    12061 caacctgttc taccaggcgt tcgataaggg cgaaatggca gacggtgaag gccgcctgat
    12121 tgactgcaaa aatatcgtct tcttcctgac gtccaacctc ggctaccagg taatagtcga
    12181 gcatgccgat gacccggaaa ccatgcagga agtactgtat ccggtgctgg ccgacttctt
    12241 caaacctgcc ctgctggcgc gtatggaagt ggtgccgtat ctgccgctgt cgaaagagac
    12301 gctcgccacc attatcgccg gaaaactggc ccgtctggat aacgtgctgc gcagtcgctt
    12361 tggtgtagaa gtggtcattg aaccggaagt gacggacgaa atcatgagcc gcgtcacccg
    12421 cgcggaaaac ggcgcgagga tgctggaatc ggtcatcgat ggcgacatgc taccgccgct
    12481 ctcgctgctg ctgttgcaga aaatggcggc taacacggcg attgcccgga ttcggttgtc
    12541 ggcagtggac ggcgcattta cggcagacgt ggaagatgct cagaacgacg agtccgtcac
    12601 aaaggatgaa acggttttat gagcaggttg cgcgttttcc ggcagacagg atggtttatc
    12661 gcgggcctga tgacgggctt acccgccact gccgcgcccg cagaagcatc gtcaatggcc
    12721 ggtgtggcgg tcgccgtcgc caccacgacc ccgccggatg cgacagccac gttacaggcc
    12781 atgcagtcct gtcggcggga gtccgcggcg ctggagcggc ttgactgcta tgaccgcctg
    12841 ctggcaccgc tgtccccgtc agggtttgac ggggcgctgg tcaaagccgg ttttgtgggc
    12901 gaggcctgga cccgcgccac agagcaggaa aaacgccgcg agggcaacac cacagaactg
    12961 ctggtgacgc aggtgccggg ggagcgcccg acggtggtga taaccacccc ggctatcggt
    13021 catgttccgc cacgcccggt gctgatgttc agttgcgtgg acaatatcac ccggatgcag
    13081 gtggcgctga tgcaccctct ggatgtccat gatatcgccg tcactctgaa tgccgacaac
    13141 cgggcattgc gcagccactg gtttgtgcgc gagaacggca ccctgctgga gtccagccgg
    13201 gggttatccg ggattgatga aattaagcaa ctgttcggcg caaaaacgct gacggtggat
    13261 accggcgcag acaatgccgc cggaaagctg acctttaaca ttgacgggct ggcaagggcg
    13321 attgctcccc tgcgcgatgc ctgccactgg gcgggagaat aaaaatatgg caatggactt
    13381 acgcgatccg aatgtctgga tatcgcacct gctggaaaac ctgccggaag agaaactggc
    13441 atcggcgctg aaagatgaca acccgaactg ggagtatatc gacggcgaaa tcgtcaagct
    13501 gggatcgctg gcccacagtc aactggatat cccggaactc cagcgccggg ggctggtcat
    13561 tctggcttcg gagagtaagg attttcgtct gctggcgcat ctgctaagaa ccctgcaaca
    13621 cgccggtgat cctcatctcg cattgcgcct gctggcgctg tatgtggagc attactgggc
    13681 ggtggctgcg ccgcagaata tggcgcataa aaaacgcttt gccagccagg tcattaaacg
    13741 ctttgaaaca ggtattgaag ttttttcaca gaacgccgcc acagcgcagc gcgatgcgct
    13801 gtcagggcaa ctggcaaaac tggcgcaatg ctggcagtca cataacgccc cggaactggc
    13861 acaggctact gatgatcttt ttgccctgta ccagcgtgcg tttaatcgtg cggcaccggc
    13921 tcctgtcccc actccggcgg cctccggtag ttcaccacag accaccgcca cgtctgaaag
    13981 cggcgtgacg caacccagtg ctccggctcc ccaaatcgtc atcgacagtc acgacgacaa
    14041 agcctggcgc gacacgctgt taaaagtggc ggccatttta tgtgaacgcc agccggattc
    14101 accgcagggc taccgtctgc gccgccatgc gctttggcag aacatcacca gtacgcccca
    14161 ggcggaaagc gatggccgta ctccactggc tgcggtctcc gccgatatgg tggcagatta
    14221 ccatgctcag ctcggtagtg ccgatatggc gctgtggcag caggtggaga aaagcgtact
    14281 gctggctcct tactggctgg atggtcactg cctttctgca cagacggcgc tgcgtctggg
    14341 ttacaaacag gtggcagacg ccatccgcga tgaggtcatc cgcttccttg agcgtctgcc
    14401 ccagcttacc gggctgctgt ttaatgacca cacaccgttt atcagtgagc agacgaaaca
    14461 atggctggca gcttcgcccg acgccaaagt tgcaccggtt gcgcaaatcg gtgaggaatc
    14521 gaaggcagcc agagcctgtt ttgctgagca gggtctggag gcggcgctgc gatatctgga
    14581 catgctaccc gaaggcgatc cccgcgatca gtttcaccgc cagtaccttg ccgcacagtt
    14641 gacggaagag gcggggctgg tacagcttgc gcagcagcag taccggatgt tgttcaggat
    14701 gggattgcag atgatggtgg cagactggga gccgtccctg cttgagcagc tagaacaaaa
    14761 attcacggca gaacaataaa agagaacagg agtttttgtg ttcagattac caacaccccg
    14821 actactcagc ggactcaaat cagccctgcg accggcgatg ccccggttta aagtctctgc
    14881 tttctggctg ctgatactgg cgtggatttt tctgctggtg tggatctggt ggaaaggccc
    14941 gatgtggacg ctgtatgaag aacagtggct caaaccactg gcgaaccgct ggctggcaac
    15001 ggcggcgtgg gggattattg ccctggtgtg gcttaccgtc cgggtgatga agcgcctgca
    15061 acagcttgaa aaaatgcaaa agcaacagcg cgaggaagcc gttgatccgc tcagcgtgga
    15121 actgaacgcc cagcagcgtt atcttgaccg ctggctgctg cgcctgcaac gccatctcga
    15181 caaccgccgt ttcctgtggc agttgccgtg gtatatggtc atcggcccgg cgggcagtgg
    15241 caaaacgaca ctgctgcgca aggggtttcc gtccgacatt atttatgccc cggagggcgc
    15301 acgcggcgca gaacaacgcc tgtacctcac gccccatgtc ggtaaacagg cggtgatctt
    15361 tgatatcgac ggcacactct gcgctcccgc tgatgcggat atcctgcatc gccgtctgtg
    15421 ggaacatgcc ctcggctggc tgaaagaaaa gcgcgcgcgc cagccgctga acgggattat
    15481 tctgacactc gatttacccg atctgctgac cgcagacaaa cgccgccgcg agcatctgtt
    15541 acagacgctg cgcagccgct tacaggatat acgccagcat cttcactgcc agttaccggt
    15601 ttacgtggta ctgacccggc ttgatttatt acagggtttt gccgccctgt tccagtcact
    15661 caacagacag gatcgcgatg cgattctggg cgtcacgttc acccgccgtg cccatgaaaa
    15721 tgatgactgg cgaacagagt tgaatgcttt ctggcagaca tgggtggatc gaatgaatct
    15781 ggcgttgccg gatctgatgg tcgctcagac tcacacccgc gcgtctttat tcagtttttc
    15841 ccgccagatg cagggaagcc gtgaaccgct ggtgtcactg cttgagggtc tgcttgatgg
    15901 cgaaaatatg aacgtgatgc tgcgtggtgt ctatctcacc tcttcgcttc agcgtggaca
    15961 gatggatgat atattcaccc agtctgccgc ccgccagtac cggctgggca ataacccact
    16021 ggcgtcctgg cccctggtgg acaccgcgcc ttatttcacc cgcagcctgt tcccgcaggc
    16081 attactcgca gagcctaatc tggcaacaga gagccgcgcc tggctgatac gttcccgtcg
    16141 ccgcctgacg gttttctctg ccacaggcgg cgtggcagca ctgctgctca tcaccggctg
    16201 gcatcactat tacaacggta actatcagtc cggcatcacc gtgcttaagc aggccaaagc
    16261 ctttatggac gtgccgcctc cgcaggggga agatgacttt ggcaacctgc aactgccgct
    16321 cctgaacccg gtacgcgatg ccacactggc ctatggcgac tggggcgacc gcagccgtct
    16381 ggccgatatg ggactgtacc agggacgacg tatcgggcct tatgtggaac agacctatct
    16441 gcaactgctg gagcaacgtt acctgccctc gctgtttaac gggctggtca aagcgatgaa
    16501 cgccgcgccg ccggagagtg aagaaaaact cgcggtgctg cgcgtgatgc gaatgctgga
    16561 ggacaaaagc ggacgtaaca atcaggtggt gaagcagtat atggcaaaac gctggagcga
    16621 aaagttccac ggtcagcgcg atatccaggc acaactgatg tcccatcttg actacgcgct
    16681 ggctcatact gactggcacg cagagcgtca ggcgggcgac ggtgacgcca tcagccgctg
    16741 gacgccatat gacaagcccg tggtatcagc acagaaagaa ctgagcaaac tgcctgtcta
    16801 ccagcgggtt taccagagcc tgaaaacgcg ggcgctgggc gttcttcctg ccgacctcaa
    16861 tctgcgtgac caggtagggc caacctttga ccaggtgttt acatctgccg atgacaacaa
    16921 actggttgtt ccacagttta tcacccgcta cggcctgcaa agctattttg taaaacagcg
    16981 cgatgaactg gttgaactga cagcgatgga ttcctgggta cttaacctca cccgcaatgt
    17041 gaaatacagt gacgccgacc gtgcggaaat ccagcaccag ttgaccgagc agtatatcag
    17101 cgactacacc gccacctggc gggctgggat ggacaatctg aatatccgca attttgagtc
    17161 catcggacaa ctgaccgggg cgctggagca ggttatcagc ggcgaccagc ctttgcagcg
    17221 ggcgctgacc gtgctgcgtg acagcacaca gccaggcgtc ttttctgaaa aactctctgc
    17281 caaagaacgg gaggaagccc tggcagagcc ggattaccag ttactcaccc gcctcgggca
    17341 tgaattcgcc ccggaaaaca gtaccctggc agtacagaaa gacaaagaaa gcacgatgca
    17401 ggccgtgtat cagcaactca ccgagttgca ccgctacctg ctggcaatcc agaacgcgcc
    17461 tgtaccaggg aaatcggcgc tgaaagccgt gcagttacgg cttgatcaga acagcagcga
    17521 tccgatattc gccacccgcc agatggcaaa aacgctgcct gctccgctca accgctgggt
    17581 tggcagactg actgactagg cctggcatgt ggtgatggtg gaggctgttc attatatgga
    17641 agtggactgg cgcgacagcg tggtgaaacc gtttaacgag caactggcaa ataactatcc
    17701 gtttaatccg cgttctgcac aggatgcctc actggatgcc ttcgaacgct tctttaaacc
    17761 ggatggcata ctggatacct tctaccagca gaacctgaag ctgtttatcg ataatgacct
    17821 gagtctggag gatggcgata acaacgtcat tattcgcgaa gatattattg cgcaactgga
    17881 aactgcgcag aaaatccgtg acatcttctt cagcaaacag aacggtctgg gaacatcctt
    17941 tgccgtggaa acggtatcgc tttcaggcaa taaacgccgc agtgtactga accttgacag
    18001 tcagttagtc gattacagcc agggccgtaa ctataccgcc catctggtct ggcctaacaa
    18061 catgcgcgaa ggcaacgaaa gtaagctgac gctcatcggc accagcggca acgcgccgcg
    18121 cagtatcagc ttcagcgggc cgtgggcgca gttccgcctg ttctgggccg gacaactgac
    18181 cggagtacag gatggcaact ttaccgtgcg ctttagcgtg gacggtggcg cgatgaccta
    18241 ccgtgtgcat accgacacgg aagataaccc gttcagcggt gggttgttca gccagtttgg
    18301 tctgtcagac acactgtact gaggaggcag ggatgaatag taacgtactg acacaaacta
    18361 tcgttaccgg cagtgacccg cgcgggctgc cggaattcag cgccatccgc gaggaaataa
    18421 acaaagccag ccacccgtca cagcctgagc tgaactggaa actggtggag tcgctggcgc
    18481 tggcgatttt taaagccaac ggtgtggatt tacacaccgc cacctactat acgcttgccc
    18541 ggacacggac acagggactg gcgggattct gcgaaggtgc ggaactgctg gcggcaatgg
    18601 taagccacga ctgggataag ttctggccgc agggcggccc ggcgcgtact gaaatgctgg
    18661 actggtttaa ctcccgaacc ggcaatattc tgcgtcagca aatctccttt gcggaatccg
    18721 acctaccact gatctaccgc acagagcggg cattgcagct tatctgcgat aagctccagc
    18781 aggtggaact gaagcgcgtt ccgcgcgtgg agaatctgct ctattttatg cagcacacgc
    18841 gtaaacggct tgaaccccag ccgaagagta acgctgagaa cgccgcacag accacggtca
    18901 gaacgctgat ttatgccccg gaaacactgg catcttccac accagaagcg gtagtgcctc
    18961 ccctgcccgg cctgcctgag atgaaagtgg aagtgcgcag tctgacagag aatcccccac
    19021 aggccagtgt gataaagcaa ggcagtacgg taagagggtt tatcgcaggg atcgcctgtt
    19081 cagtggctgt cgcctcagca ttgtggtggt ggcaggtcta tccggtgcag cagcaactgt
    19141 tacaggttaa cgacaccgct cagggcgcag caacggtgtg gatggcctca cctgaactcg
    19201 aaaactatga gcgcaggctg caacaacttc ttgatacctc cccggtacag ccgctggaaa
    19261 ccgggatgca gatgatgcgt gttgccgaca gtcgctggcc ggaaagcctg caacagcaac
    19321 aggcctcgac acaatggaat gaggcactca aaacccgcgc acagagtagc ccgcagttgc
    19381 gtggctggtt gcagacccgc caggacttac atgcttttgc agatctagtg atgcagcgcg
    19441 agaaagaggg actaaccctt tcctatatca aaaatgtcat ctggcaggcg gagcggggac
    19501 tggggcagga aacccccgtt gagtctctgt tgacgcagta ccatgatgcc cgtgcgcaga
    19561 agcagaatac agatgcgctg gaaaaacaaa ttaatgagcg actcgaaggc gtgttaagcc
    19621 gctggctgct gctgaagaat aacgtcatgc cagaggcggc aaccggtacc acggccgaaa
    19681 aataattatt taattaaagg agattatcat ggcaaatata agttatttat ctttgagtgg
    19741 agagacacaa gggctaattt cagcaggttg ctcaacgctt gattctgtcg gaaacaaagc
    19801 gcagccagaa cataaagatc agatcatggt ttatgcgtta atgcattcca tctcaagaag
    19861 tcaaaatgtc aatcatcatg aattaattat caccaaaccg gttgataaat cctcaccatt
    19921 gctggcaaaa gcacttagtg acaatgaaaa aatggcaata tgtgaattta tactctaccg
    19981 aaccagtaaa gcgggtattt accagcctta ttataaaata aatctgtcaa aagcccgaat
    20041 atcaagtatt gattttgtca caccgcatgc tgtcctggaa aaagaactgg aaccacagga
    20101 gagaattgcc tttatttatg aagatatttc ctgggagcat acgttagccg gtacgaatgc
    20161 aatgagtaaa tggcaggatc gtgtacagta acttactgta ataacataat aaattactat
    20221 tgcggagaga taaaatggct ggtttaagga ataacaacaa cacacagaat gcacagtggg
    20281 ctgattatgt gggtgacatt cttcgggggg ctcagccgat aaaccagcta gtacctcagc
    20341 acccttatct taacgacgtc ccactcatt
//
LOCUS       DAZOYS010000061        20212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOYS010000061 DAZOYS010000000
VERSION     DAZOYS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.061"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..692
                     /locus_tag="ACHUN9_001934"
                     /pseudo
     CDS             1..692
                     /locus_tag="ACHUN9_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(757..1468)
                     /locus_tag="ACHUN9_001935"
                     /pseudo
     CDS             complement(757..1468)
                     /locus_tag="ACHUN9_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(1468..2709)
                     /gene="clsB"
                     /locus_tag="ACHUN9_001936"
     CDS             complement(1468..2709)
                     /gene="clsB"
                     /locus_tag="ACHUN9_001936"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0695338.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2706..3467)
                     /locus_tag="ACHUN9_001937"
     CDS             complement(2706..3467)
                     /locus_tag="ACHUN9_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0695339.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3600..4010
                     /locus_tag="ACHUN9_001938"
     CDS             3600..4010
                     /locus_tag="ACHUN9_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0695340.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3972..5078)
                     /locus_tag="ACHUN9_001939"
     CDS             complement(3972..5078)
                     /locus_tag="ACHUN9_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0695341.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5089..6231)
                     /locus_tag="ACHUN9_001940"
     CDS             complement(5089..6231)
                     /locus_tag="ACHUN9_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0695342.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6224..7960)
                     /locus_tag="ACHUN9_001941"
     CDS             complement(6224..7960)
                     /locus_tag="ACHUN9_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0695343.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7953..8948)
                     /gene="hlyD"
                     /locus_tag="ACHUN9_001942"
     CDS             complement(7953..8948)
                     /gene="hlyD"
                     /locus_tag="ACHUN9_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0695344.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8951..9622)
                     /gene="cecR"
                     /locus_tag="ACHUN9_001943"
     CDS             complement(8951..9622)
                     /gene="cecR"
                     /locus_tag="ACHUN9_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0695345.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9851..11215
                     /gene="rhlE"
                     /locus_tag="ACHUN9_001944"
     CDS             9851..11215
                     /gene="rhlE"
                     /locus_tag="ACHUN9_001944"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0695346.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11447..11929)
                     /gene="ybiA"
                     /locus_tag="ACHUN9_001945"
     CDS             complement(11447..11929)
                     /gene="ybiA"
                     /locus_tag="ACHUN9_001945"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0695347.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12049..14199
                     /gene="dinG"
                     /locus_tag="ACHUN9_001946"
     CDS             12049..14199
                     /gene="dinG"
                     /locus_tag="ACHUN9_001946"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0695348.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14227..15189
                     /gene="ybiB"
                     /locus_tag="ACHUN9_001947"
     CDS             14227..15189
                     /gene="ybiB"
                     /locus_tag="ACHUN9_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0695349.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            15330..16415
                     /gene="hcxB"
                     /locus_tag="ACHUN9_001948"
     CDS             15330..16415
                     /gene="hcxB"
                     /locus_tag="ACHUN9_001948"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0695350.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16482..16541
                     /gene="ybiE"
                     /locus_tag="ACHUN9_001949"
     CDS             16482..16541
                     /gene="ybiE"
                     /locus_tag="ACHUN9_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0695351.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16644..16904)
                     /gene="ybiJ"
                     /locus_tag="ACHUN9_001950"
     CDS             complement(16644..16904)
                     /gene="ybiJ"
                     /locus_tag="ACHUN9_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0695352.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17169..17435)
                     /gene="ybiI"
                     /locus_tag="ACHUN9_001951"
     CDS             complement(17169..17435)
                     /gene="ybiI"
                     /locus_tag="ACHUN9_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0695353.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17509..18186)
                     /gene="ybiX"
                     /locus_tag="ACHUN9_001952"
     CDS             complement(17509..18186)
                     /gene="ybiX"
                     /locus_tag="ACHUN9_001952"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0695354.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSASLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18228..20212)
                     /locus_tag="ACHUN9_001953"
                     /pseudo
     CDS             complement(18228..20212)
                     /locus_tag="ACHUN9_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4666 a         5305 c         5484 g         4757 t
ORIGIN      
        1 gcaataaatt agattttcag cacgaggcca ggatataaaa cggtatatgg ctcattaccg
       61 ctttaggttt ggtggcaacc gcaggactag cctggggagc taagtatctc gaaattacgg
      121 caaccaaata tgattcacca ccaatgtatg tcgctatagg gttattatta ctttgtatgt
      181 atggcttaag taaggatatc aacaagataa atgccgccat cgcgggcgta atatatctgt
      241 ttttactctc tttggtggcg attgtcgttg caagtttagt tcctgtatat gccattatca
      301 tcgtgttcag cactgcgggc gcgatgtttt taatcagtat gctggccggt ttattattta
      361 atgttgagcc tggttctcac cgttttatca ttatgatgac gttgacaggg ttggccctgg
      421 taatcatcgt gaatgcggca ttaatgagtg aacggcccat ttgggtaata agttgcttaa
      481 tgattgtgtt atggccaggc attatctcgc atggacgaaa taagctcctt gaactggcag
      541 gaaaatgcca tagtgaagag ttgtggagtc cggttcgttg cgcttttaca ggtgcattaa
      601 cactctatta ctattttatc ggcttctttg ggatacttgc cgcgatagct ataacgcttg
      661 tctggcaaag gcatacgtgt ttttttcatt agccgtaaat gcacatacta aagaacttaa
      721 ctaaacttca catcgccgct tcatttttcg cccgtagccc agtacaaaga aatagttcac
      781 gctttgacca agtaacagcc agataatcgc ccccattacc atccagttaa cgctggaaat
      841 cagcatctga gcgagggcga atttccatga aggcagcacc agcttttgcc ctttgatggt
      901 catatggcgg tgcttcgcga aagcgcaaaa ccacaaataa accgcgataa tcatcagtaa
      961 gccaatgccg agaatacgca gcgtagtttg atcgacatac cagtgatccg gcaactccac
     1021 cacgcctgcg gtaaagataa tccctgccag taaaatatag cccagccagt tggtggtaat
     1081 actgagcgag aaaatccgcg taatagtgct accgggcaac cccagacgag agtacaaacg
     1141 atagcgcata ccaatgccgc cgacccaggt actgagcgtc aggttgaagg cgtagcagat
     1201 aaacgacacc agcatcacct ggcgcttcgc cagtttgtga ccgcagtaaa agcgggcgag
     1261 caggtcatag cagccgtaaa tcagatagct gacgaccacc agcgaccgca ctaagcagcg
     1321 caacgcgatt gtagtcgcgg atgaccttcc agacctcttc ccagtccact tttttggcgt
     1381 agaccaccag caacacaatc accgcgataa aaaacagcca ggtgaggatc ttctttgcta
     1441 agcgccagcg cgggtgtgat ttactcatca gggttttacc cccgtgtttt cagtttctac
     1501 ccgatcctgc gtttccattg tcggttgtgc gggcggatca acctgcgcca gacgtggcgt
     1561 gtgtgccgga agccagccaa ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac
     1621 gcttttggtc aggttccacc aggtgcgttt gggcagcatg gtttcatcca cctgctgaca
     1681 atctgcggcg ataatgccgt tcagattatc gcgcagcgtc tggttaaaat tacgatcgtg
     1741 gatgatgaca tttgcttcga gattcagtga caaactgagc ggatcgagat tactggaccc
     1801 tactgtcgcc cagtgatcgt ccatcaatgc tactttgccg tggagcgggc ggcggcggta
     1861 ctcaaacacc tgaacgccgc ctttaaccag atagttatac agcaagcgcg cgccgactct
     1921 gacaatcggc atatccggtt cgccctgaat gatcagtttg atccgcaccc cgcggcgtgc
     1981 cgctttacgc aaggcgtgta aaaatcgata gccggggaag aagtaggcgt tggcgataat
     2041 gacttcccgc cgcgcctgag tgagcatttt cagatagtgg cgttctatat catcgcggtg
     2101 ttcttcgtta tcgcgccaga ccagcaatac ctgcgcgtct cccggctggc ggttctcttc
     2161 cgctttgtga tgacgtcgcc accagcgtcg tgccgcgctc tgtccaggca ggttttccag
     2221 ctcaaactgg agaatatctt cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt
     2281 agcctctgga ccgtagctgg acatatgctc ggcggagtaa ttcagcccgc caataaaggc
     2341 gatacgcgcg tcgatcacca caattttgcg atgcatccgg cgaaacacat tggtgcgcat
     2401 accaaaaagg cgagggcggg gatcgtagta gcggaacact acgccagctg ccgtcagttc
     2461 attgacaaac tcatcgctga gatccggcga accgtagcca tccagcaaga cttccgcttt
     2521 aaccccgcgt tgcgctgctg ccagtagtgc cgcatgcagc tgtttgccga cgttatcctc
     2581 aaaccagata aacgtttcaa gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac
     2641 cgcgggataa tattgctcgc cgttttccag caactggatc ttattgcctt cgcgccagct
     2701 acatttcata aatgaatctc cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc
     2761 cgcagcggca acgcggttgg cgcgctggcg ctggcatttt tgacgtagat cctgtccagt
     2821 cgtagtagag gaaattgcac cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa
     2881 atctcatcca gtccggcctg cacttttaac ggatgattag ctttttgccg ccagtcattg
     2941 aaatcacccg ccaccaatac cggttcgccg tccggtagct cattcaccca ttcggcgagc
     3001 atcgcaagct gcgcctgacg gtgcgcctca cgcaggccca gatgtacgca catcacatga
     3061 atcgcttttc cggtcatcgg cggcacaatg cggcagtaga gcacgccgcg cttttccgca
     3121 ccatcgaccg aaacatcgcg attctcataa tgttcaatgg gataacgcga cagtacggcg
     3181 ttgccgtgat gcccttccgg gtatacggca ttgcgaccgt aggcaaaatc gctccacata
     3241 gtgtcggcga gaaactcgta gtgcgaggta tcgggccagt tttccacatg cagcggatga
     3301 acttcgtgcg cgcccatcac ttcctgcagg caaacaatat cggcgctgac ggtacgcacg
     3361 gcatcgcgaa gttccggcaa aatgaagcgt cggttaaacg cggtaaagcc tttgtgaatg
     3421 ttgatggtga gcaccttgaa cgaaaattgt tgtgtttgat cgggcataat tttcctgtct
     3481 ttgcctctta tctcattgaa atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga
     3541 attttccgta aagttcggta ctctgagtaa gtagagataa attcttcagg agagaagcca
     3601 tgaagtggca acaacgtgtt cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc
     3661 atttactggt agtggcgctg ctggtggtgg gctggatgag taagactctg gttcacgtcg
     3721 gcgtgggatt atgcgcactg tattgtgtca cggtagtgat gatgctggtg tttcagcgcc
     3781 accccgagca acgctggcgt gaggtggcag acgtgctgga agagctgacc acgacctggt
     3841 attttggcgc agcgctgatt gtgctgtggc tgttgtcccg cgtactggaa aacaactttt
     3901 tgctggcaat tgcagggctg gcgatccttg ctggcccggc ggtagtgtct ttgctggcga
     3961 aagataagaa gttacatcac cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg
     4021 gccgttatca ccagtagcgg ccacaaacta ttccacacaa tatccagact cgcatccttc
     4081 aaataaatct gcttggtaat gtccgtaaag tggcgaatag ggttaatcca cgtcaggttt
     4141 tgcagccata ctggcatgtt ttccaccgga gaaacgtaac cggaaaggag aatggcgggc
     4201 atcataaaaa caaacacgcc gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc
     4261 aacagaccga atcccaccag cgataaaccg taaatcacca tcgtaaagta gaacagcgcc
     4321 agcgatccgg cgaaggggat ttgatacgcc cagataccaa tcgccagcac aatggtggcc
     4381 tggaaggtgg cgacaattaa cgccggtacg gctttgccga tgaagatctg ccaggtggtg
     4441 agcggcgaaa ccagtagctg atcgagcgta ccttgttcac gttcgcgggc gacggaaagt
     4501 gaagtgacga tcattacgcc gatagtggtg atcatggcga tcagcgacgg caccacaaac
     4561 catttgtagt caagattcgg gttataccag ttgcgtacca ccagctcgct gttgttaggt
     4621 ttcggttttc cttccagcag ctcctgctga taatttttga cgatctgttg caggtagttg
     4681 gcggcaattt gcgcactgtt ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc
     4741 tggaaggtat ccagtttgcg cgagaagtca gccgggaaac gcaccagtag taacgccttt
     4801 tgtgtgtcga tggttgggcg gatctcctgt gggcttttca gcagcagcac atgggtaaag
     4861 gcgctggcgc gggcaaaacg ttgggtcagc tccaccgaat gctcgccgtt atcttcatcg
     4921 tagatggcga tggtggcgtt agtcacttcc agcgtggcgg cgaacgggaa caggatcacc
     4981 tgaataagca caggtaaaat cagaatcgcg cgggtttgtg gttcgcgcag caacgactgc
     5041 aactctttgc ggattaacgt ccataagcga tgaaacatgc tcttctccct aatccagccg
     5101 acgtttggtt ttcagccacg tcaggccgat aaacatcacc gccgaagcga tcaaaaacag
     5161 cacgtttacc accagcacca ctggaatatt cccggcgagg aacaggcttt gcagggtgct
     5221 gacgaaataa cgagcgggaa taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc
     5281 gatctgaaaa ataaagccgg aaagcataat cgacggcagg aacgcggcgt tcagggcgac
     5341 ctgagcggca ttgaactggt tgcgggtaat cgtggaaatc agcagcccca tccccagggt
     5401 actgagtaaa aacaggctgg agataaaaaa cagaataagc agcgaaccgc gatacggcac
     5461 gccgagaata aacactgaca ccagcataca cagcagcatc gccagcatcc cgagaaagta
     5521 ataagggatc agcttacaca gcagcagttc cgtgcgggta atctccgtag agagcagagc
     5581 ctccatggtg ccgcgttccc attctcgcgc caccaccagc gaggtgagaa tcgcgccgat
     5641 gaccgtcatg atgatggtca ctgcaccggg gataatgaag tgctggctaa tcgccgccgg
     5701 gttaaaccag tagcgggttt gtacatcaat aagcggttca aaagtctgcc cgttgtcctc
     5761 cgctcgctgc atttgccaga tctgccagat ctgccagatc ccttcgacat acccctgtac
     5821 aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt
     5881 ggcgcgctcc atctgttccg caaaatccac cggaataacc accagaccgc gaattttccc
     5941 cgcctgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg
     6001 cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagcaagat
     6061 cccgacccgc agcttgctgg agtcgaggtt aatgccataa ccaaaaataa acagcagtag
     6121 cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc
     6181 tttgacgcac agcgcccgca cgcgacgcca ggaaaggatc gggttactca ttgctatgct
     6241 ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct
     6301 catcgttagc cgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt aatttcccac
     6361 ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca
     6421 tcaccgtgac gcctttctct accatgctgt tgatatgtag ccaaaattca cggcgggtga
     6481 ggggatcaac gccggacgtc ggttcgtcga gaaacagaat gtccggttca tgcatcagcg
     6541 aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg gcgtgggagg
     6601 cgatactttt caggccgaac gcctcgctca tgcgggagat tttttcgttc tgcgcccgac
     6661 cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttaccgt
     6721 agagcgaaaa cttttgcgcc atatagccga gatgctggcg cgctttacca gaactctctt
     6781 tcagatccat ccccagtacc agcgcctgac cagaggtcgg caccagcaaa ccgcacatca
     6841 tcttaaaggt ggtcgatttg cccgcgccgt ttggccccag caaaccaaaa atctccccgc
     6901 gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt
     6961 ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgtaat attgcgccca
     7021 gcggcgattc cgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg
     7081 gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata tcgtctggtg
     7141 tggtctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct
     7201 gcggcagttt caatgcccgt tgcaacagtt tgcggttgcc ctcgtgtgga cttgtcatca
     7261 gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttccccctga tacagcaact
     7321 cgccttcatt catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac
     7381 tccagaggat taacatccct tcgcctgcca gctcatgcac catctgccac agttcgcgcc
     7441 gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca
     7501 ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc
     7561 gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg
     7621 cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat
     7681 cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt
     7741 ttttgattgg atcaaagcca atcaccgtgg cattgccgct gtcgggtttc agtaatcccg
     7801 ccagcatccg cattagcgtg gttttacctg caccatccgg ccccaccagc cccgtcacat
     7861 aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc
     7921 gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc gttaccgaat
     7981 tgcaccgtca ccggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc
     8041 aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca
     8101 gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta
     8161 taaagcagca ctttgcgccc cggctgggcc tggtcaagat accgttcatc aacataagcg
     8221 cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgaggacc
     8281 gtgcctggct ccaccgcgcg cgttaacagc gtgccatcag acggggctat caacgttgag
     8341 tcctgtaaat tcaactccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc
     8401 gcctgggcga tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct
     8461 gatttcagcg ttgcctgcgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc
     8521 gaaatagtgc ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca
     8581 taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac
     8641 ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc
     8701 agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca
     8761 tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact aagattcacc
     8821 gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac
     8881 cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagttcgat cacgacaggt
     8941 tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg
     9001 tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta
     9061 acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggtat
     9121 gaaggatcat gcgggtgtca ttggcgtcgc tgccggtcca ggcggcaatc aggcgtgtca
     9181 ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag
     9241 agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct
     9301 taatcatgtt cctgcaggcg cgaaggagca gttcacggat ggcagcccga tcaggctgtg
     9361 gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat
     9421 cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga
     9481 tggcagcgat attctgcccg gcttgggcgg ctatctcgcg ggtggtggcg ttcattccat
     9541 attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga
     9601 ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt
     9661 atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg
     9721 ataatttatg cgctgcgtca caaaaactgc tacactccgc tcgctcatga cattgtggtt
     9781 tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt
     9841 cggagtagtc atgtctttcg attatttggg tttaagccct gatatcctgc gcgccgttgc
     9901 cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga
     9961 aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct
    10021 gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg
    10081 tgcgctcatt cttaccccga cccgtgaact ggcggcgcag attggcgaaa acgtccgtga
    10141 ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggtggtgtga gtattaaccc
    10201 gcagatgatg aaactgcgtg gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct
    10261 ggacctggaa catcagaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga
    10321 agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact
    10381 acctgcgaag cgccagaacc tgttattctc cgcgaccttc tctgacgata ttaaagccct
    10441 ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc
    10501 tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc
    10561 gcacatgatt gggaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg
    10621 cgctaaccat ctggctgaac agctcaataa agatggcatc cgtagtgcgg cgatccacgg
    10681 caataaatcg caaggtgcgc gtactcgtgc gctggctgat tttaaatcgg gcgatattcg
    10741 tgtactggtg gcaactgaca tcgctgcgcg cggcctggat attgaagagc tgccgcacgt
    10801 cgtcaactat gaactgccaa acgtaccgga agattatgtc catcgtatcg ggcgtaccgg
    10861 tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct
    10921 gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga
    10981 gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcgg
    11041 cggtcgtggg caaggtggtg gtcgcggtca acagcaacca cgccgtgggg aaggtggcgc
    11101 aaaatctgca agcgcgaaac ctgcagaaaa accgtctcgc cgcctcggcg atgccaaacc
    11161 ggcaggcgaa caacaacgtc gccgccgtcc gcgtaaacct gccgctgcgc agtaatcttt
    11221 tatgccgggc tatgcccggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta
    11281 cgcttatcag gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg
    11341 ataaggcgtt tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac
    11401 acgggaaaaa atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag
    11461 ccaattgttc tcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac
    11521 caccgtctcc ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg
    11581 ccagcaagag cacacgcagt tctgcatgct gttcgaattt agcacgaagt gctttacgca
    11641 tcacctgttc tttaaccgac tcccagtttt tacgaagtgg cttggaacga ttacgcccca
    11701 tgcgagcggc gaccatgggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa
    11761 ggaatttttg tgcctgaaaa tagtgttcgg aggtgggcca ggttttcccg tcaaccttga
    11821 tgggccaggc ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta
    11881 tgatggtgtc ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt
    11941 atgttggtgg ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
    12001 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
    12061 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
    12121 tttattcccc gtgtgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
    12181 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat
    12241 ttgatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
    12301 aacgtggcgt tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt
    12361 cccgatctta aattcactgc cgctttcggg cgtgggcgct acgtttgtcc gcgtaatctg
    12421 acggcgctcg ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa
    12481 ctgacggcaa ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
    12541 acttatagat gggatggtct gcgcgatcat acggatatcg ctatagatga tgatctctgg
    12601 cgtcgtttga gtaccgacaa agccagctgc ctcaaccgta actgttacta ctatcgtgaa
    12661 tgcccgtttt ttgttgctcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat
    12721 gcgctggtga tggcggcgat ggaaagcgaa gccgtactgc ctgacccgaa aaatctgctg
    12781 ctggtgctgg acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagc
    12841 gctgaaatca ccgcgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct
    12901 acctgcatgg agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg
    12961 aatgcgcatt gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc
    13021 tacatgcctg ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa
    13081 gtgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
    13141 ttatttctta acgatttaag tgagaaaacc ggcagccatg acattgtacg cctgcatcgg
    13201 ttgattttgc agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg
    13261 ctggcgtctc tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag
    13321 cgcgaagggc aactgcatct ctggtttcac tgcgtgggga tacgtgtcag cgatcagctg
    13381 gaaaggctgc tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg
    13441 ctgaatagtt tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt
    13501 tttgtggcgc tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
    13561 atgcgcgttg agccttccat cgacaacgaa gagcagcata ttgccgaaat ggcggccttt
    13621 ttccgtaagc aggtggagag caaaaaacat ctcggtatgt tggtactgtt tgccagcgga
    13681 cgggcgatgc agcgctttct cgactatgtg acggatttac gtctgatgtt gctggttcag
    13741 ggcgatcagc cgcgttaccg tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag
    13801 cgcagcgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
    13861 ctcagccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc cgtggtgatc
    13921 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
    13981 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
    14041 ggcgaagtgg ttatctacga caaacgcttg ctgaccaaaa attatggcaa gcgactactg
    14101 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
    14161 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
    14221 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
    14281 gatttagacc gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct
    14341 gaccttgagt tgggcggcgt attgattgcg ctgcgtatca aaggagaagg ggaagcagag
    14401 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gccagcaggc
    14461 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg
    14521 ccgttgttgg cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc
    14581 gaagatccaa cccgcgtgct gactgaaacc atttttgaat tgatgggtat tacgccaacg
    14641 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgccagtc
    14701 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
    14761 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtgc ggcgctgcgt
    14821 ctttccagcg tttcgcatcc ggaatatatt ggacgcgtcg cgaagttctt tagcgatatc
    14881 ggcgggcggg cgctactaat gcatggtact gaaggtgaag tgtatgctaa tccgcagcgc
    14941 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
    15001 actgctggta gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggatt
    15061 gagcgttgcc ttgctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggtttgc
    15121 tgcctggtgg ctatgggtga agcggcaact atcagcgacg gcctggcgcg cgttaatcag
    15181 gcattttaat tcttttctca gccggatgag ccatgtcatc cggcttttac cccaccgcaa
    15241 tatgaaattc ctgcatcttt attgaccttc ccacgcccgg cgtgcagcat aaaaatacaa
    15301 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
    15361 cgctgcacag ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
    15421 aattagttgc cgatcattta atcgcggcaa acctggcagg gcatgattca catggtattg
    15481 gcatgatccc aagctatgta cgctcctgga gtcaggggca cctgcaaatt aaccatcatg
    15541 ccaaaaccgt taaagaggcg ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc
    15601 aggtcgcggc acatgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
    15661 ccgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
    15721 gtgcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
    15781 cgccgttcca cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc
    15841 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa
    15901 cccgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
    15961 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct
    16021 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
    16081 ccggcggtaa aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact
    16141 gcatgaccac tatcatcatc aacccggaac tcttcggcgc gccggattgt aacgcgcaga
    16201 ccgaagcctt tgccgagtgg gtgaaagcct cgccacatga tgatgataag ccgattttgc
    16261 taccgggcga gtgggaagtg aacacgcgtc gcgaacggca gaagcagggg attccactgg
    16321 atgcgggaag ctggcaggcc atttgtgatg cagcgcggca gattggtatg ccggaagaga
    16381 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
    16441 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
    16501 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
    16561 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt
    16621 aaacgcctta tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg
    16681 ttgttggtgg cggaagtgat gctgtatcca ctggcaccgg ctgccgcggc tttctcagcc
    16741 agtttcgctt ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta
    16801 ttcatgttct gtgcctggga tgccgttatc ggttccgccg cgaacacgcc aaatgacaga
    16861 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
    16921 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
    16981 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
    17041 ctgataaacc atttatttgt tgtacttatg aacttgtttt ataccgcgtg gcagtcacag
    17101 cagcgttaaa ggcacggaat gccacgcaaa ataaaaatta tactcgctat gcatctcgcc
    17161 agttgtagtt aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtataagct
    17221 ggtttttgta aatctttctc ctgctgacaa tgaatacata agcgcacgcc aggaatggct
    17281 tcccgacggg cctgcgggat gggggcaccg cactcttcac attcatccag gctttcgccg
    17341 cgcggaattt caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg
    17401 ttgacggcgt catcgttagc ccaaccggat gccatatcga cctccccata tcaatacttg
    17461 tacagttaag tgtagctaat ccagggacga actcgggcag ttcaagcatc agatctccga
    17521 ccattcccgc agcagattat gataaagatt aagcagcgac aggatctctt cactttcgcc
    17581 gtagcggctt ttcagcgact gaatattgtt gtccagttca aacagcatgg cgcgcttttt
    17641 atcatcgcgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
    17701 gggtggcacg caatgctggc tgctggaggg atacaacacg agatcgcctg ccgggagttt
    17761 tacccgatgt tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
    17821 atcgcttaaa aacagcgagg cagaaaggtc agtacgcatc cagccgtttt gtggatggct
    17881 gcgtactgcg ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag
    17941 cggcgtggaa agggtacgcg gcaaggccgc ggcaaagaac aaagcatgtt ggttaaccgc
    18001 gttcagcacc tcattttgca gggcggcgta taacgtgctg cgggtgtcga cctgttgatt
    18061 gttcttaact tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg
    18121 ttccagttgt tcgcgaaacc gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
    18181 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
    18241 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg
    18301 attgaggcga cgtaatcagt atcaaacagg ttgtagacgt ttagctggaa gtcgagattg
    18361 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttcggt aaatgctggc
    18421 gttcccaccg cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca
    18481 acagagatat cgtcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta
    18541 tacggcagcg atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt
    18601 tgctgggtat agccgccaat cacctgccac gcgggagtga tattcccggc cacggatatc
    18661 tcatagcctt cgacgcgttt cttaccgtat tgcgagtaag ttccgtcatc attttgctca
    18721 acttcatttt cgatatcagt acggaacagc gcggcggtga gcaacagacg tttatccaga
    18781 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
    18841 ttggcactgt taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
    18901 tgggaaacgg catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg
    18961 gctttccagt tcatcagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct
    19021 tttgccacac cagttgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
    19081 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
    19141 gtgatttgca gcgtatcaaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
    19201 gcgccgttgc gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc
    19261 gcgggtaacg tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg
    19321 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
    19381 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacca ggttcagcta
    19441 ttcacatcgc tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg
    19501 taatcctgct ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg
    19561 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
    19621 ccgtaaaagt tatgagtatc aacttttccg gaatgattca gggccgccgt tcccgcagat
    19681 ggggcagaat agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctgg
    19741 gtgacatgca gataattaag atacaaacga ttcgctgtac caaggccaaa agcgacagaa
    19801 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg
    19861 cccattacat tcaggcgcac cgcggtggta tcaccaatga cctgattgac gtccagcgtg
    19921 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
    19981 ggctgtttgc tgatcatatt gatcgagccc gtcggtgcgc tgcgcccgta gtcggtgccg
    20041 gacggccctt taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg
    20101 atatcgcgaa tgccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg
    20161 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gc
//
LOCUS       DAZOYS010000062        20132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOYS010000062 DAZOYS010000000
VERSION     DAZOYS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.062"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(196..1008)
                     /locus_tag="ACHUN9_001954"
     CDS             complement(196..1008)
                     /locus_tag="ACHUN9_001954"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0695355.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1270..2031
                     /gene="udp"
                     /locus_tag="ACHUN9_001955"
     CDS             1270..2031
                     /gene="udp"
                     /locus_tag="ACHUN9_001955"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0695356.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2172..3599
                     /gene="rmuC"
                     /locus_tag="ACHUN9_001956"
     CDS             2172..3599
                     /gene="rmuC"
                     /locus_tag="ACHUN9_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0695357.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3694..4449
                     /gene="ubiE"
                     /locus_tag="ACHUN9_001957"
     CDS             3694..4449
                     /gene="ubiE"
                     /locus_tag="ACHUN9_001957"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0695358.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4463..5068
                     /gene="ubiJ"
                     /locus_tag="ACHUN9_001958"
     CDS             4463..5068
                     /gene="ubiJ"
                     /locus_tag="ACHUN9_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0695359.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5065..6705
                     /gene="ubiB"
                     /locus_tag="ACHUN9_001959"
     CDS             5065..6705
                     /gene="ubiB"
                     /locus_tag="ACHUN9_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0695360.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6784..7053
                     /gene="tatA"
                     /locus_tag="ACHUN9_001960"
     CDS             6784..7053
                     /gene="tatA"
                     /locus_tag="ACHUN9_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0695361.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7057..7572
                     /gene="tatB"
                     /locus_tag="ACHUN9_001961"
     CDS             7057..7572
                     /gene="tatB"
                     /locus_tag="ACHUN9_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0695362.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7575..8351
                     /gene="tatC"
                     /locus_tag="ACHUN9_001962"
     CDS             7575..8351
                     /gene="tatC"
                     /locus_tag="ACHUN9_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0695363.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8393..9175
                     /gene="tatD"
                     /locus_tag="ACHUN9_001963"
     CDS             8393..9175
                     /gene="tatD"
                     /locus_tag="ACHUN9_001963"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0695364.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9172..9660)
                     /gene="rfaH"
                     /locus_tag="ACHUN9_001964"
     CDS             complement(9172..9660)
                     /gene="rfaH"
                     /locus_tag="ACHUN9_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0695365.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9827..11320
                     /gene="ubiD"
                     /locus_tag="ACHUN9_001965"
     CDS             9827..11320
                     /gene="ubiD"
                     /locus_tag="ACHUN9_001965"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0695366.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11367..12068
                     /gene="fre"
                     /locus_tag="ACHUN9_001966"
     CDS             11367..12068
                     /gene="fre"
                     /locus_tag="ACHUN9_001966"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0695367.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12258..13421)
                     /gene="fadA"
                     /locus_tag="ACHUN9_001967"
     CDS             complement(12258..13421)
                     /gene="fadA"
                     /locus_tag="ACHUN9_001967"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0695368.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13431..15620)
                     /gene="fadB"
                     /locus_tag="ACHUN9_001968"
     CDS             complement(13431..15620)
                     /gene="fadB"
                     /locus_tag="ACHUN9_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0695369.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15810..17141
                     /gene="pepQ"
                     /locus_tag="ACHUN9_001969"
     CDS             15810..17141
                     /gene="pepQ"
                     /locus_tag="ACHUN9_001969"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0695370.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17141..17755
                     /locus_tag="ACHUN9_001970"
     CDS             17141..17755
                     /locus_tag="ACHUN9_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0695371.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17794..19245
                     /gene="trkH"
                     /locus_tag="ACHUN9_001971"
     CDS             17794..19245
                     /gene="trkH"
                     /locus_tag="ACHUN9_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0695372.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19257..19802
                     /gene="hemG"
                     /locus_tag="ACHUN9_001972"
     CDS             19257..19802
                     /gene="hemG"
                     /locus_tag="ACHUN9_001972"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0695373.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4703 a         5106 c         5482 g         4841 t
ORIGIN      
        1 tgggagttga tggtaaatct ctaaggtgtc ttccattgaa gagactcatt tatgactatg
       61 acttgccgac tttcttaagg tcaaatagcg gagtcgtaat ctccaatcaa ttcatactga
      121 ttattttttg ggggacagag atggcagcaa aacgcactcg tttactcacc atctcaaata
      181 tagactaaga ggaaactaac tccgcttctc tccatactgc ttaaaccatt ccagcatacg
      241 ctgccagcca tctttcgcag attcggcatg atagctcggg cgataatcag cgttgaatgc
      301 atgcccggca tccgggtaca cgataatctc tgctttcgca ttagcagccc gcagcgcctg
      361 gcgcatggtt tcaacgctct cctgcggaat gctgttatcc tgaccgccat ataagccgag
      421 aatcggcgcg ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat tcagcgactt
      481 gtcgcccgtc agtttgccgt accacgccac tgcagctttt agctgtggat tatgcgcggc
      541 atacagccag gtgatacgtc caccccagca gaatccggtg atcattaaac gatgaacatc
      601 gccgccgttg cgggaagccc aactggcggc atgatcgaga tcggccagca cctgcgagtc
      661 aggcactttt gctaccagac cgctaagcaa cgtggggata tcggcaaaat cattcggatc
      721 gccttcgcgg aagtaaagtt caggtgcgat agccagatac ccctccagcg ccagacggcg
      781 acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc tgcactacaa tgaccactgg
      841 cagtgggcca tcgctttgct ttggtctggc atggtaagca ggcatgttat ccccttgtga
      901 agggatagag gtgaagcccg ccacaatcgc gtcgtccagg gtctgaacga tggtcgaagc
      961 gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg tattctccgt
     1021 acccttataa aaatgttgcg caatgttaac tatagtcagc atgcaacaaa tcacattgcc
     1081 tgaatcggct catcttttat gcagtcctgc agaatgaaga gtgatttatg tgatttgaat
     1141 cacttttggt gggtaaattt atgcaacgca tttgcgtcat ggtgatgagt atcacgaaaa
     1201 aatgttaaac ccttcggtaa agtgtctttt tgcttcttct gactaaaccg attcacagag
     1261 gagttgtata tgtccaagtc tgatgttttt catctcggcc tcactaaaaa cgatttacaa
     1321 ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat cgccgcgctg
     1381 atggataagc cggttaagct ggcatctcac cgcgaattca ctacctggcg tgcagagctg
     1441 gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac ctctattgct
     1501 gttgaagagc tggcacagct gggcattcgc accttcctgc gtatcggtac aacgggcgct
     1561 attcagccgc atattaatgt gggtgatgtc ctggttacca cggcgtctgt ccgtctggat
     1621 ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg tcgctgattt cgaatgtacg
     1681 actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa ctcacgttgg cgtgacagct
     1741 tcttctgata ccttctaccc aggtcaggaa cgttacgata cttactctgg tcgcgtagtt
     1801 cgtcacttta aaggttctat ggaagagtgg caggcgatgg gcataatgaa ctatgaaatg
     1861 gaatctgcaa ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg tatggtagcg
     1921 ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg ctgagacgat gaaacaaact
     1981 gaaagccaag cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta attctcttct
     2041 cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct cgcaggaaat
     2101 gcctttccaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt
     2161 caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcgttg gtgggtgtgg
     2221 caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg
     2281 aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc caaagcgagc
     2341 actggcgtgc agagtgcgag ttactcaata acgaagtgcg cagcctgcaa agtattaaca
     2401 cctctctgga ggccgatctg cgtgaagtaa ccacgcggat ggaagccgca cagcaacatg
     2461 ctgacgataa aattcgccag atgattaaca gcgagcagcg cctcagtgag cagtttgaaa
     2521 acctcgccaa ccgtattttt gagcacagca atcgccgggt tgatgagcaa aaccgtcaga
     2581 gtctgaacag cctgttgtcg ccgctacgtg aacaactgga cggtttccgc cgtcaggttc
     2641 aggacagctt cggtaaagaa gcacaagaac gccataccct gacccacgaa attcgcaatc
     2701 tccagcaact caacgcgcaa atggcccagg aagcgatcaa cctgacgcgc gcgctgaaag
     2761 gcgacaataa aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt
     2821 ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct
     2881 cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg aaaagatgtg gtgatcgacg
     2941 ccaaaatgac gctggtcgcc tatgaacgct attttaacgc cgaagacgac tacacccgcg
     3001 aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca
     3061 aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg tttattcccg
     3121 ttgaacccgc ttttttactg gcgcttgacc gccagccgga gctgatcacc gaagcgttga
     3181 aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgttgcgt actattgcca
     3241 acctgtggcg ttatgagcat caaagccgca acgcccagca aatcgccgat cgtgccagca
     3301 agctgtacga caagatgcgt ttgttcatcg atgacatgtc cgcgattggt caaagtctcg
     3361 acaaagcgca ggataactac cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg
     3421 tgctggcgca ggcagaagcg tttcgcggtt taggagtaga aattaaacgc gagattaatc
     3481 cggatttggc tgaacaggcg gtgagccaga atgaagagta tcgacttcgg tcggttccgg
     3541 agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc
     3601 ccattagggg tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact
     3661 tctggaacaa ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg
     3721 cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc
     3781 cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcatttgg tattcatcgt
     3841 ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat
     3901 ctggctggtg gcaccggcga cctgacagcg aaattctccc gcctggtcgg agaaactggc
     3961 aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggacgcga gaagctgcgt
     4021 aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc gctgccgttc
     4081 ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa
     4141 gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt
     4201 gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat
     4261 gtgctgccgc gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca
     4321 gaatccatcc gtatgcatcc cgatcaggat accctgaaag ccatgatgca ggatgccgga
     4381 ttcgaaagtg tcgactacta caatctgacg gcaggggttg tggcgctgca tcgtggttat
     4441 aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag
     4501 tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acagcccgct cgcgtctgct
     4561 gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag
     4621 cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc
     4681 ctacgccagt gtgttgccga aacttcgcga tcgccagcag cttaccgcac tgattcgcag
     4741 tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg cgctggcaga
     4801 tctggcagag ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga
     4861 aggaatcagc aaagccatgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca
     4921 gcaacgttat gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga
     4981 agtggcctgg tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa
     5041 acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc
     5101 attcgcactt ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg
     5161 ccgctacggc tatggcgata ctcattattc tggatgccaa atcggcataa agacaaactt
     5221 ttaggtgagc gactacgact ggccctgcaa gaactggggc cggtttggat caagttcggg
     5281 caaatgttat caacccgccg cgatcttttt ccaccgcata ttgccgatca gctggcgtta
     5341 ttgcaggaca aagttgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca
     5401 atgggcggct tgccggtaga agcgtggttt gacgattttg aaatcaagcc actggcttct
     5461 gcttctatcg cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt
     5521 aaagtcatcc gcccggatat tttgccggtt attaaagcgg atctgaaact tatctaccgt
     5581 ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg
     5641 gtgcgcgagt acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaac
     5701 gccattcagc ttcggcgaaa ttttgaagac agcccgatgc tctacatccc ggaagtttac
     5761 cctgactatt gtagtgaagg gatgatggtg atggagcgca tttacggcat tccggtgtct
     5821 gatgttgcga cgctggagaa aaatggcacc aacatgaaat tgctggcgga acgtggcgtg
     5881 caggtgttct tcactcaggt ctttcgcgac agctttttcc atgccgatat gcaccctggc
     5941 aacattttcg taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg
     6001 attgttggct cgctaaacaa agaagataaa cgctatctgg cagaaaactt tatcgccttc
     6061 tttaatcgcg actatcgcaa agtggcagag ctacacgtcg attctggctg ggtgccacca
     6121 gataccaacg ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc tatctttgag
     6181 aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt
     6241 cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctatgtc
     6301 gaaggggtag gacgccagct ttatccgcag ctcgatttat ggaaaacagc gaagcctttc
     6361 ctggagtcgt ggattaaaga tcaggtcggt attcctgccc tggtgagagc atttaaagaa
     6421 aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg aattggttta cgacagtttg
     6481 cgccagggca agtatttaca gcacagtgtt gataagattg cccgcgagct tcagtcaaat
     6541 catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt
     6601 ggcacattct tgttggtcag ccgacctgaa tgggggctga tgcccggctg gttaatggca
     6661 ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca
     6721 aggcgggtcg tgtaacgtat aatgcggcct tgtttaatca tcatctacca cagaggaaca
     6781 tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt
     6841 tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt
     6901 aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagtcagga tgctgatttt
     6961 actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa
     7021 gacgcgaagc gccacgataa agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa
     7081 ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg
     7141 gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac
     7201 gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag
     7261 gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaact acgccaggcc
     7321 gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg
     7381 cacaccatcc ataacccggt ggtgaaagat aatgaagctg cgcatgaggg cgtaacgcct
     7441 gccgctgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg
     7501 gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt
     7561 gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctaattgagc
     7621 tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt gatattcctg tgtctggtct
     7681 atttcgccaa tgacatctat cacctggtat ccgcgccatt gatcaagcag ttgccgcaag
     7741 gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct
     7801 ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcatttatcg
     7861 ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc
     7921 tgctgtttta tatcggcatg gcattcgcct actttgtggt ctttccgctg gcatttggct
     7981 tccttgccaa taccgcgccg gaaggggtgc aggtatccac cgacatcgcc agctatttaa
     8041 gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg
     8101 tgctgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg
     8161 tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa
     8221 cgctattggc gatcccgatg tactgtctgt ttgaaatcgg tgtcttcttc tcacgctttt
     8281 acgttggtaa agggcgaaat cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa
     8341 ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga
     8401 tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg
     8461 cgcttttgac gcgggagtta atgggctact catcaccggc actaacctgc gtgaaagcca
     8521 gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc
     8581 tcacgacagc agccagtggc aagctgcgac tgaagaagcg attattgagc tggccgcgca
     8641 gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc
     8701 ggaagagcag gaacgcgctt ttgttgccca gctacgcatt gccgcagatt taaacatgcc
     8761 ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct
     8821 ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc
     8881 gtgcgtggcg catggaattt atatcggcat taccggttgg gtttacgatg aacgacgcgg
     8941 actggagctg cgggaacttt tgccgttgat tccggcggaa aaattactga tcgaaactga
     9001 tgcgccgtat ctgctccctc gcgatctcac gccaaagcca tcatcccggc gcaacgagcc
     9061 agcccatctg ccccatattt tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg
     9121 gctggctgcc accacggatg ctaatgtcaa aacacggttt gggattgcgt tttagagttt
     9181 gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat taagcaatag
     9241 catggagcga gcctcaccat cgggttcggt gaaaatggcc tgaaagcctt cgaacgcgcc
     9301 ttcggtaata atcaccttat ctcccggata aggggttgcc ggatcgacaa tgtctttcgg
     9361 tttatatacc gatagctgat gaatcaccgc cgatgggact atcgctagcg acgcgccaaa
     9421 gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttctgg
     9481 atcaaattcg acaaacaggt agttgggaaa caatggctca ctgactgcag tacgttttcc
     9541 acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct gtctctcaag
     9601 gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc aagattgcat
     9661 aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg ctaagttaat
     9721 tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa atttacagca
     9781 tcgcaaagat gaacgccgta taatgggcgt agattaagag gctacaatgg acgccatgaa
     9841 atataacgat ttacgcgact tcttgacgct gcttgaacag cagggtgagc taaaacgtat
     9901 cacgctcccg gtggatccgc atctggaaat cactgaaatt gctgaccgca ctttgcgtgc
     9961 cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa
    10021 cctgttcggt acgccaaagc gcgtggcgat gggcatgggg caggaagatg tttcggcgct
    10081 gcgtgaagtt ggtaaattat tggcgtttct gaaagagccc gagccgccaa aaggtttccg
    10141 cgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga caaagcggct
    10201 gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat
    10261 tcccattatg acctgctggc cggaagatgc cgcgccgctg attacctggg ggctgaccgt
    10321 aacgcgcggc ccgcataaag agcggcagaa tctgggcatt tatcgccagc agctgattgg
    10381 taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgctgg attatcagga
    10441 gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc
    10501 cgccacgatt ctcggtgcag tcacccccgt tccggataca ctttcagagt atgcgtttgc
    10561 cggattgctg cgcggtacca agaccgaagt ggtgaagtgt atctccaatg accttgaagt
    10621 gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga
    10681 agggccgtat ggcgaccaca ccggttacta taacgaagtc gatagtttcc cggtatttac
    10741 cgtgacgcat attacccagc gtgaagatgc gatttaccac tccacctata ctggtcgtcc
    10801 gccggatgaa cccgcggtgc tgggtgtcgc actgaacgaa gtgtttgtgc cgattctgca
    10861 aaaacagttc ccggaaattg tcgattttta cctgccaccg gaaggctgtt cttatcgtct
    10921 ggcggtagtg acaatcaaaa aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt
    10981 ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg atgatgacgt
    11041 caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg acccggcgcg
    11101 ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct cgcctgtctc
    11161 cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccggggg aaacccagcg
    11221 tgaatgggga cgtcccatca aaaaagatcc tgatgtcgtc gcgcatattg acgccatctg
    11281 ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgttag ttttgcccta
    11341 tttatttgat ccgacagaga aagcgcatga caaccttaag ctgtaaagtg acctcggtag
    11401 aagctatcac ggatactgta taccgtgtcc gaatcgtacc agacgcggcc ttttcttttc
    11461 gtgctggtca gtatttgatg gtagtgatgg atgagcgcga caaacgtccg ttttcaatgg
    11521 cttcgacgcc ggatgaaaaa gggtttatcg agctgcatat tggcgcttct gaaatcaacc
    11581 tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg gtcgatattc
    11641 cccacggaga agcgtggctg cgcgatgatg aagagcgtcc gatgattttg attgcgggcg
    11701 gcaccgggtt ctcctatgcg cgctcgattt tattgacggc gttggcgcgt aatcccaatc
    11761 gcgatatcac catttactgg ggtgggcgtg aagagcaaca tctgtatgat ctctgcgagc
    11821 ttgaggcgct ttcgctgaag catcctggtc tgcaagtggt gccggtggtt gaacaaccgg
    11881 aagcgggctg gcgtgggcgt actggcaccg tgttaacggc ggtcttgcag gatcatggta
    11941 cgctggcaga gcatgatatc tatattgccg gacgttttga gatggcgaaa attgctcgtg
    12001 acctgttttg cagtgagcgt aatgcgcggg aagatcgtct gtttggcgat gcgtttgcat
    12061 ttatctgaga tataaaaacc cgcccctgac aggcgggaag aacggcaact aaaactttat
    12121 tcagtggcat ttagatctat gtcgcatccg gcaatcggtg cacgatgcct gatgcgacgc
    12181 tggcgcgtct tatcaggcct acattggtgc aggatcggta gaccggataa ggcgttcacg
    12241 ccgcatccgg caagtggtta aacccgctca aacaccgtcg caataccctg acccagaccg
    12301 atacacatcg tcgccagacc aaactgaacg tctttgtgtt ccatcagatt cagcaacgtg
    12361 gtgctgatac gcgcaccgga acaacccagc ggatgaccca gcgcgatcgc accaccattg
    12421 aggttgatct tctcgtcaat ctgctccatt agcccgagat ctttaatgca cggcaggatc
    12481 tgcgcggcaa aggcttcgtt catctcaaac acgccgatat cgctggcaga aagccccgct
    12541 tttttcagcg ccagcttcga ggcaggaacc gggccgtaac ccataatcga cggatcacaa
    12601 ccaacgaccg ccatcgaacg cacacgggcg cgcggcttaa gacctaattc acgggcgcgg
    12661 ctttcactca tcaccagcat ggccgcggca ccatcagaaa gtgcagaaga tgtacccgcc
    12721 gttaccgtac cgcttactgg atcaaacgcc ggacgcagcg tggcgagggc ttccacggtg
    12781 gtttccgggc gaatcacttc gtcgtaatta aactgcttca ggacgccgtc ggcatcgtga
    12841 ccaccggtcg ggatgatttc atttttaaat gcgcccgact gcgtggcggc ccaggcgcgg
    12901 gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc tgataccgtg catacgcgcc
    12961 agcatttctg ccgttaagcc catcatgccc gccgctttgg cgacattgcg gctcaggccg
    13021 gggtgaaaat cgacgccgtg actcatcggc acatggccca tatgctccac gccgccaacc
    13081 agacatgcct gcgcatcgcc agtcatgatc attcgtgctg cgtcatgcag tgcctgcatg
    13141 gatgaaccac acaagcgatt gacggtaacc gccgggacag agtgcggcac ttctgccagc
    13201 agcgccgcgt tacgggcgat attaaaaccc tgctccagcg tctgctgcac acaaccccag
    13261 taaatatcgt cgagggccgc cgcttccagc gccgggttac gcgccagcag gctacgcatt
    13321 aaatgagcgg agagatcttc tgcacgcacg ttacgaaaag caccgccctt cgaacggccc
    13381 atcggggtgc gaattgcatc gacaatgaca acctgttcca ttgtgactcc ttaagccgtt
    13441 ttcaggtcgc caaccggacg ggctggctca accggaggat agtacggttc gttatgacgc
    13501 gctttattac gcagaccttc cggcacttca tacagcgggc cgaggtgctg atattgctgt
    13561 gccatatcga ggtattttgc gctaccgagg gtgtccagcc agcggaacgc gccgccgtgg
    13621 aacggaggga agcccaggcc gtagaccagc gccatatccg cttccgccgg agtggcgata
    13681 atgccctctt ccagacagcg caccacttcg ttgaccatcg ggatcatcat gcgggcgata
    13741 atctcttctt cgctgaaatc gcgcttcggc tggctcactt ctgccagcag gtcttcaacg
    13801 gcggcgtctt cttctttctt cggcttacct ttgctgtctt ctttataacg ccagaaaccg
    13861 aggccgttct tctgaccaaa gcggttggca tcaaacagcg cgtcgatggc atcgcggtaa
    13921 tctttttgca tccgctgcgg gaagcctgct gccatgacag cctgagcgtg atgcgcggta
    13981 tcaatgccca caacgtccag cagatatgcc gggcccatcg gccagccaaa ctgtttttcc
    14041 atcactttgt cgatcttgcg gaaatccgcg ccgtcgcgca gcagctggct gaaaccggcg
    14101 aaatacggga acagcacgcg gttaacaaag aagccggggc agtcgttaac cacaatcggc
    14161 gtcttgccca tcttgctcgc ccaggcgaca actttcgcga tggtttcgtc ggagcttttc
    14221 tcgccgcgaa taatttctac caacggcatt cggtggaccg ggttaaagaa gtgcatcccg
    14281 cagaagtttt ccgggcgttc cagcgcgttg gccagttcgc tgataggaat ggttgaagtg
    14341 ttagacgcca gcacggtatc ctggcgtact ttttgttcgg tttctgccag tacggctttt
    14401 ttcactttcg ggttttcaac aaccgcttct accacaatat ccacgcggtc aaatccggcg
    14461 tagtcgagcg ttgggtggat cgtggagatc acgccagcca gtttcagacc atcgatcttg
    14521 ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg tcatgctgag ggttaacgac
    14581 ttgtcgttga tatctttcat gacaaccggc acgcctttcc acgcggactg gtaagcgata
    14641 ccgccaccca tgatgcctgc gcccagcaca gcagcctgtt tcggggtttc aacgtctttg
    14701 gtgagtttct tcgctttgcc ttttacatat tgatcgttaa ggaaaatgcc gaccagtgcg
    14761 cgagcttcgt tggtatgcgc cagcgggaca aaacttttgt tttccagatt taaggcttct
    14821 tcacgaccaa aacgggccgc agcttcaatg gtttttactg cggtgatggg ggccggataa
    14881 tgtttccccg ccgtttgcgc gaccattcct ttagcgatgg tgaagctcat ggtggcttca
    14941 atcttgctca gttttagcgg ttccagcttc ggctggcgtt tcgctttcca gtcgaggtcg
    15001 ccgttaatgg cctggcgtaa aaccgccttt gcgccttcaa ccagtttttc tgctttgact
    15061 accccatcca ccagaccgat tttcagcgcc tgatccgcgc cgacatcttt accggcggca
    15121 atgatttcca gcgcactgtc agcgcccagc atacgtggca tacgtacaga accgccaaag
    15181 cccggcatga tgcccagttt ggtttccggc agaccgatgc gcagatccgg cgtcgccaga
    15241 cgataatcgg tcgccagcac gcattcgcag ccaccaccca gcgcatagcc gttgacggca
    15301 gcaatggtcg gcaccggcag atcttccagg cgattaaaca cgctattggc aaagtgcagc
    15361 cactgactta actgttcttc aggaacgagg aacagggaca aaaattcggt gatatcagca
    15421 ccgacgataa aggctgcttt gttcgaacgc agcagcagcc cttttagatc tgattgctgt
    15481 tccagcacgc cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag tttattaact
    15541 gaacctgggg catcaaatac cagttcggca atgccatctt ccagccagtc aaggtacagg
    15601 gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg gtcgtaccag
    15661 atgcatcgaa gtgtgtattt tgtgttaaaa atatgcaaat aaaagattaa agaaatgccg
    15721 atctgatcac gctcggcaga aatcacgctc tggatgaacg atgtgctaag atgcggagac
    15781 ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact ggcctcgctc tataaaaatc
    15841 atatagctac cttacaagaa cggactcgcg atgcgctggc gcgcttcaag ctggatgcat
    15901 tacttattca ctccggcgag ctgttcaacg tttttctcga cgatcatccc tatccgttta
    15961 aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac tgctggttgc
    16021 tggtggatgg cgtgaacaag ccgaaactgt ggttctatct gccggttgat tactggcaca
    16081 acgtcgaacc gctgccgacc tccttctgga ctgaagatgt ggaagtgatc gcactgccga
    16141 aagccgatgg cattggtagc ctgctgcccg ctgcgcgcgg caatatcggt tatatcggtc
    16201 cggtgccgga acgtgcgctg caactgggta ttgaggccag caacatcaac ccgaaagggg
    16261 tgatcgacta cctgcattac taccgctcct tcaaaaccga gtacgaactg gcctgtatgc
    16321 gtgaagcgca gaaaatggcg gtcaacggtc atcgtgcggc agaagaagcg ttccgttctg
    16381 gcatgagcga gttcgatatc aacatcgcct atctgaccgc gaccggtcat cgtgataccg
    16441 acgtacctta cagcaacatt gtggcgctta acgaacacgc ttcggtgctg cattacacca
    16501 aactggatca tcaggcaccg gaagagatgc gcagcttcct gctggatgcc ggggccgaat
    16561 ataacggcta tgcggctgac ctgactcgta cctggtcggc aaaaagcgac aacgactacg
    16621 cacagctggt gaaagacgta aatgatgaac aactggcgct gatcgccacc atgaaagcag
    16681 gcgttagcta tgtggattac cacatccagt tccatcagcg catcgccaaa ctgctgcgta
    16741 aacatcaaat catcaccgat atgagtgaag aagcgatggt cgaaaacgat ctcaccggac
    16801 cgtttatgcc gcatggtatc ggtcatccgc tgggcctgca ggtgcatgac gtagccggtt
    16861 ttatgcagga tgatagcggt acacacctcg cggcaccggc aaaatatccg tacctgcgct
    16921 gcacccgtat tctccagccg ggtatggtgt taaccatcga accgggtatc tacttcattg
    16981 aatcgctact ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac tggcagaaaa
    17041 ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga caacgtggtg atccacgaaa
    17101 acaacgtgga aaacatgacc cgggatctga aactggcgtg atggaaagct ggttaattcc
    17161 tgcggcaccg gtcacggtcg ttgaagagat caaaaaaagc cgcttcataa cgatgttggc
    17221 gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg cagaacaccc
    17281 cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt ctcaacagct
    17341 gggtttctct gacgacgggg agccggcagg aacggcaggt aaaccgatgc tcgcccagct
    17401 aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg cgctactacg gcggcatatt
    17461 gcttggcacc ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg cgctgcgcca
    17521 gctaacgacc caacgcaaga cgccattaac cgaatatact ttgcaatgtg aatatcatca
    17581 gttaaccggc attgaagcgt tgctggggca gtgtgacggc aaaattatca acagtgatta
    17641 tcaggcattc gttctgctgc gggtggcgct tccggcggcg aaagtggctg aattttccgc
    17701 aaagctggcg gattttagcc gtggttcatt gcaattgtta gcgattgaag aataatcccc
    17761 acttcgtttt gcagaactaa ggaagcggca gagatgcatt ttcgcgccat tacccgaatc
    17821 gttggactac tggtcatctt attttcaggg accatgatta tccctgggct ggtagcactc
    17881 atctaccggg atggagcggg ccgcgctttt acccagacct tttttgtcgc cctcgccatt
    17941 ggttctatgc tgtggtggcc gaaccgtaaa gagaagggcg aactgaaatc ccgtgagggg
    18001 tttctgatag tggtgctgtt ctggaccgtg ctgggtagtg tcggtgcgct cccttttatc
    18061 ttctcggaaa gcccgaacct cacgattacc gatgcgtttt ttgaatcttt ctctggcctg
    18121 accaccaccg gggccactac gctggtgggg ctggattcgc tccctcatgc catcctcttt
    18181 tatcgccaga tgctgcaatg gtttggcggg atggggatca tcgtgttagc ggttgcgata
    18241 ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc cggcccgctg
    18301 aaagataaca aaatgcgccc gcgaattgcg gaaacggcga aaaccctgtg gttgatttat
    18361 gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga tgcctttgat
    18421 gccatcggcc atagctttgc gactatcgct attggcggct tctcgacaca tgatgccagt
    18481 atcggttatt tcgacagccc gactattaac actatcattg ctatcttcct gctgatctcc
    18541 ggctgtaact acggtctgca cttttcactg ttaagtgggc gtagtctgaa ggtttattgg
    18601 cgcgatccgg aatttcgcat gtttatcggc gtacagttta cgctggtggt tatttgtaca
    18661 ctcgtactgt ggtttcataa tgtctacagt tcggcgctga tgacaattaa ccaggcgttt
    18721 ttccaggtgg tatcgatggc gacaaccgcc gggtttacga ctgacagcat tgcccgctgg
    18781 ccgctctttt tgccggtact gcttttatgt tcagctttta tcggcggttg cgccgggtca
    18841 acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg gaaccgtgag
    18901 ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta agctggggaa tcgcgcactg
    18961 ccggaacgta tcctcgaagc cgtttgggga tttttctccg cctatgcatt ggtgtttatt
    19021 gtcagtatgc tggcgattat cgccacgggc gtggatgact tttctgcctt tgcttcagtt
    19081 gttgcgacat tgaataacct ggggccaggg cttggcgtgg ttgctgataa ctttaccagt
    19141 atgaacccgg tggctaaatg gatcctgatt gccaacatgc tgtttggtcg tctcgaggtc
    19201 tttacattgc tggtgctctt taccccgact ttctggcgtg aatgatggag taatacgtga
    19261 aaacattaat tcttttctca acaagggacg gacaaacgcg cgagattgcc tcctacctgg
    19321 cttcggaact gaaagaactg gggatccaga cggatgtcgc caatgtgcac cgcattgaag
    19381 aaccacaatg ggaaaattat gaccgtgtgg tcattggtgc ttctattcgc tatggtcact
    19441 accactctgc gttccaggaa tttgtcaaaa aacatgcgac gcgactgaat tcgatgccga
    19501 gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga gaagcgtact ccacagacca
    19561 acagctacgc gcggaagttt ctgatgaact cgcaatggcg tcccgatcgc tgcgcggtca
    19621 ttgccggggc gctgcgttac ccacgttatc gctggtacga tcgttttatg atcaagctaa
    19681 ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga agttgtctat accgattggg
    19741 agcaggtggc gaatttcgcc cgagaaatcg cccagttaac caacaaaccg acgctgaaat
    19801 aagcataaag aataaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc
    19861 cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
    19921 gtcagcgggg ttctcctgag aattccgaca gagaaagcaa aaataaatgc ttgactctgt
    19981 agcgggaaag cgtattatgc acaccccgcg ccgctgagaa aaagtgaagc ggcactgctc
    20041 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    20101 agacgaaaaa tgaataccaa gtctcaagag tg
//
LOCUS       DAZOYS010000063        20128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOYS010000063 DAZOYS010000000
VERSION     DAZOYS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.063"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            201..872
                     /locus_tag="ACHUN9_001973"
     CDS             201..872
                     /locus_tag="ACHUN9_001973"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0695374.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            882..2078
                     /locus_tag="ACHUN9_001974"
     CDS             882..2078
                     /locus_tag="ACHUN9_001974"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0695375.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2078..2638
                     /locus_tag="ACHUN9_001975"
     CDS             2078..2638
                     /locus_tag="ACHUN9_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0695376.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2640..3413
                     /locus_tag="ACHUN9_001976"
     CDS             2640..3413
                     /locus_tag="ACHUN9_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0695377.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3601..3876
                     /gene="frmR"
                     /locus_tag="ACHUN9_001977"
     CDS             3601..3876
                     /gene="frmR"
                     /locus_tag="ACHUN9_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0695378.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3911..5020
                     /gene="frmA"
                     /locus_tag="ACHUN9_001978"
     CDS             3911..5020
                     /gene="frmA"
                     /locus_tag="ACHUN9_001978"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0695379.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5113..5946
                     /gene="fghA"
                     /locus_tag="ACHUN9_001979"
     CDS             5113..5946
                     /gene="fghA"
                     /locus_tag="ACHUN9_001979"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0695380.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6172..6711)
                     /locus_tag="ACHUN9_001980"
     CDS             complement(6172..6711)
                     /locus_tag="ACHUN9_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0695381.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHUN9_001981"
     CDS             complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHUN9_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0695382.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHUN9_001982"
     CDS             complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHUN9_001982"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0695383.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYLSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHUN9_001983"
     CDS             complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHUN9_001983"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0695384.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHUN9_001984"
     CDS             complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHUN9_001984"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0695385.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHUN9_001985"
     CDS             complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHUN9_001985"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0695386.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHUN9_001986"
     CDS             complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHUN9_001986"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0695387.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12917..14581)
                     /locus_tag="ACHUN9_001987"
     CDS             complement(12917..14581)
                     /locus_tag="ACHUN9_001987"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0695388.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14623..15456
                     /locus_tag="ACHUN9_001988"
                     /pseudo
     CDS             14623..15456
                     /locus_tag="ACHUN9_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15533..16615
                     /gene="lacI"
                     /locus_tag="ACHUN9_001989"
     CDS             15533..16615
                     /gene="lacI"
                     /locus_tag="ACHUN9_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0695389.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHUN9_001990"
     CDS             16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHUN9_001990"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0695390.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19864..>20128
                     /locus_tag="ACHUN9_001991"
     CDS             19864..>20128
                     /locus_tag="ACHUN9_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0695391.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4737 a         5519 c         5223 g         4649 t
ORIGIN      
        1 tacataccaa acatacaaac acttaacata tgaattgcat aaaaacatta cccagtcgaa
       61 cctccgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat ttataaatta
      121 acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt agtataataa
      181 ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt tcttcggcaa
      241 ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa ttaggctgtg
      301 gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagcagtg gtgatgacta
      361 ccggcaactc tggtacagat ggaataatcg atcgccatga agaatcgcgc aacgccctaa
      421 agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc gctcatttac
      481 agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt ccttctgatg
      541 ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat catctcgctg
      601 tttatcaagc ttcaatggtt gcctgccgca ctattccaca aattctcggc tacgaaaccc
      661 caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa gaatatttca
      721 cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgtgat tatatgcgtc
      781 atgatcgcct gcgtgcagtt gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg
      841 aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc tggatattta
      901 tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt aaacccagtc
      961 aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg tgtctggcgc
     1021 agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc gttaacgacg
     1081 gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct
     1141 ttgttgccgt cacgcaaaaa aataccggta aaggtggtgc gctgatgaat ggcctcaatt
     1201 atgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg cccgatcaag
     1261 acggaatggg ctatatgctg gcagaaatta agcgcggtgc tgatgccgta ggcggcattc
     1321 cctctactgc gttgaaaggc gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc
     1381 cgatgattgt tatgaagcgc acgctacagc agctcctggg cggcgcaccg tttattatca
     1441 gcggtgcctg cggaatgttc cgtactgatg tattgcgtaa gttcggtttc tcggatcgta
     1501 ctaaagtcga agaccttgat ctcacctgga cattagtggc aaacggctac cgtattcggc
     1561 aggcgaatcg ctgcatcgta tacccacagg aatgcaacag tccgcgtgag gagtggcgtc
     1621 gctggcggcg ttggattgtg ggctacgcgg tctgtatgcg cctgcataaa aggcttttat
     1681 ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgatt tatggcgttg
     1741 ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt
     1801 tggcaatgtt tccgcttatc tggatcggcg tagtttgtgt tattggtgct tttagcgcct
     1861 ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg tatgtattat
     1921 tagcttatgc catctggatt atctatggac ttattgcctt ttttactgga cgcgaacctc
     1981 agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct tattcccaac
     2041 cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa aaattattca
     2101 gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac
     2161 ccgtatttgt catggtgccg ttattcaggg tccggtagtg attggcgcaa actgcctgat
     2221 aggtaattat gcgtttattc gtcctggcac aataatcagc aatggtgtaa aaattggttt
     2281 tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc cgcaatgtta
     2341 tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac gtaccagtaa
     2401 tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg
     2461 atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac aagttattat
     2521 tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg
     2581 taatttacct accggaactt attcactccg acaagaactt atccgtacag gagattaaaa
     2641 tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct tatgccgggt
     2701 taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat ggcaaccgta
     2761 atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt
     2821 cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt cagggtgcgg
     2881 tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt gcggataata
     2941 cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg ttgccaaaaa
     3001 cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa gtcgatgcct
     3061 ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc tatacccatt
     3121 atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc
     3181 cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggcgct tacacctatg
     3241 actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtttgcaa cgtattcaac
     3301 cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac gacaccccaa
     3361 ccgatgatta taacggtctg caacttgcag cccatctgac ctggaaattc tgattgcttc
     3421 tgccgcccgc tatccggggc ggccttccct gccgattagc cccactccct ttcctcgttg
     3481 ttttccgacc acattcaccg gataaatttt attctccagc gttatatact atagggaggt
     3541 atgcattgac atatagcata cctccctata gtatattgcg tgcagatgat gaggtgcgaa
     3601 atgcccagta ctccggaaga gaagaaaaag gtccttactc gagttcgtcg tattcggggg
     3661 cagattgatg ctctggaacg gtcgctggag ggtgatgccg aatgccgtgc catactccaa
     3721 cagatcgctg ccgttcgggg cgcggctaac gggctgatgg cagaagtgct tgaaagccat
     3781 atccgggaaa cgtttgaccg aaatgactgc tacagccgcg aagtcagcca atccgttgac
     3841 gacactattg aactggttcg agcctatctt aaatagctga atctattacc atattgagga
     3901 agagcgagag atgaaatcac gtgctgccgt tgcatttgct cccggtaaac cgctggaaat
     3961 cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg ctgattaaag tcacccatac
     4021 cggcgtttgc cataccgacg catttaccct ctccggggat gacccggaag gtgtattccc
     4081 ggtggttctc ggtcacgaag gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag
     4141 cgtcaaacct ggcgaccatg tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt
     4201 ctgtcgttct ggcaaaacta acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt
     4261 gatgccagac ggcaccaccc gtttttctta caacgggcag ccgctttatc actacatggg
     4321 gtgctctaca ttcagtgaat acaccgtggt cgcggaagtg tctctggcca aaattaatcc
     4381 agaagcaaac catgaacacg tctgcctgct gggctgtggc gtgaccaccg gtattggcgc
     4441 ggtgcacaac acagctaaag tccagccagg tgattctgtt gccgtgtttg gtcttggcgc
     4501 gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat
     4561 cgataccaac ccgaagaaat tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa
     4621 cccgaatgac tacgacaaac cgattaaaga tgtcctgctg gatatcaaca aatggggtat
     4681 cgaccatacc tttgaatgca tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc
     4741 gcaccgcggc tggggtcagt cggtgatcat cggggtggca ggttctggtc aggaaatctc
     4801 cacccgtcca ttccagttgg tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt
     4861 gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg atgaaaggtg atatcgatct
     4921 ggaaccgttt gtcacgcata ccatgagcct tgatgaaatt aatgacgcct tcgacctgat
     4981 gcatgaaggc aaatccattc gaaccgtaat tcgttactga tttcccgcag gtataccccg
     5041 tccacttcag acggggttct taatactctc cccgggcagc cgtccggggg attaaccgtg
     5101 agataatgac tgatggaact cattgaaaaa catgccagct ttggcggctg gcaaaatgtg
     5161 tatcggcatt attcccaatc actgaaatgt gaaatgaatg tcggcgtcta tctcccacca
     5221 aaagccgcga atgaaaaatt gccggtgctg tactggcttt caggcctgac ctgcaacgag
     5281 cagaatttca ttactaaatc ggggatgcag cgttacgcgg ctgagcacaa cattattgtt
     5341 gttgcgccgg acaccagtcc gcgaggcagt catgtcgcag atgccgaccg ttacgatctc
     5401 gggcaaggtg ccgggtttta cctgaacgca acgcaagcgc cgtggaatga acattacaaa
     5461 atgtatgact atatccgcaa cgagctgccg gatttagtga tgcaacattt tccggcaacg
     5521 accagaaagt ctatctctgg tcattctatg ggcgggctgg gcgcgctggt gctggcgtta
     5581 cgtaacccag atgaatatgt cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa
     5641 gtaccgtggg gacagcaagc ctttgccgca tatcttggtg aaaataaaga tgcctggttg
     5701 gattacgatc cggtgagtct tatttcacaa ggtcaacgcg ttgcggaaat catggttgat
     5761 caggggttga gtgatgattt ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc
     5821 tgccaggaga tgaatatcaa gacgttaatc cgttatcacg agggttacga ccacagctat
     5881 tattttgtct ccagttttat tggcgagcat attgcctacc acgccaataa actgaatatg
     5941 cgttgataat agtgcacgac tgccggatgc ggcgtgaacg ccttatccgg cctacacttc
     6001 gcccgtaaac cgtaggcctg ataagacgcg caaagcgtcg catcaggcat ggcgcacgac
     6061 tgccggatgc ggcgtgaacg ccttatccgg tctacacttc gcccataaac cgtaggcctg
     6121 ataagacgcg catagcgtcg catcaggcat ctgcgcacga cttatctatg tttaccacat
     6181 caaatcatca ggcactttga agtcggcata cggatcgtct tcatcctgct cttcggcact
     6241 gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat cggcaacact
     6301 ggcgggaata atggtgtatt caccttcgct attgttatcg accaacaagc gggcaatcgc
     6361 cagacgacca ttaatcaact gcgcctgtgt gagcttatcg acaaaaatct tcttaatcag
     6421 attgccgtca gtgaagttaa aaccaatatc gccattggca atggtgattc ggttcatttc
     6481 aatgagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt tttgctgttc
     6541 gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg cagcccgagc
     6601 ttcacgcgcc tgaacgcgcg attttttcgc cgttctctcg accttcgccg cttttttgct
     6661 ggtgactaat cctgctttga gcaactgctc ttgtaaggta agttttgcca tttttgtttc
     6721 caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtgggtg tattgggggc
     6781 aagtcccttt cgcccctgct taataaggcg cgtcaggcat cggcacacgg ctgcattctt
     6841 gatctccgac tcatcaaaat aaacaccgcc aacccagcaa caagaatacc cggtgcagaa
     6901 gcggccatta cgccgaccgt gccagtgcct aatgccagca ttttcccggc cagtaacgga
     6961 ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt tgcgcggatc
     7021 tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc tgtcgcaaac
     7081 aaccccgcga caaatcccgc cagcaacata ccgttaaatg acgacaccgt tccaagcgcc
     7141 agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg acgcagctta
     7201 tccatcaatg cgcccaacat taacgtcccg cttgccgccc ccatttgcag ggcaaacatc
     7261 acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagtagcgg tagccagttg
     7321 atcaacatat aaaccaccag cagagtgaag aaataacaca accacagcag cagcgtcgcg
     7381 gttgccgttt ctggcgcaaa taaggcacgc agtggtggcg cagcctgttt ttcgccagcg
     7441 aaaaccgctg actccggcag ccagcgcatt aatagcggca ccagaatcaa cggcaccaca
     7501 ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc cagtgtcgcc
     7561 gccagcgccg cgccaatggg aacaccgcaa tacatcaggc tcactgccgt cccacgaaaa
     7621 cgtggacccg cggcttcaga tgtcagggcg ataagattcg gcaacgccgc ccccagcccg
     7681 acaccggtca tcagccgcgc aaagaccagt gaggggaaat cccaggcaat cgccgttgcc
     7741 agtgagaaca aaccaaacag cgcaactgag ccaatcaaaa tacgcttacg accataacgg
     7801 tctgccagca tcccgccaac caacgcgccg ggtagcaaac cgagtattcc ggcgctaaat
     7861 atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc cgccgcaatg
     7921 ccagccgcct gaagatccag cccttccatc agagcgacca aaaaacaaag cccgatggtc
     7981 agcatcaggc gggatgaaga tgatgaaggg gtacgagtcg acataagatg aacctcattg
     8041 cgagtgcagg tgaatcgcga ctgatacatt gccagccgcg taatcagaac tatttaatga
     8101 ctgcctacgg gcgaaatgta gcgaaatgta ggccggataa ggcgttcacg ccgcatccgg
     8161 cagtcgtgcg cagatgcctg atgcgacgct aacgcgtctt atcagaccta caaattctca
     8221 cgccatccgt aggccggaca aggcgttcac gccgcatccg gctgtcgtgc tgggatgggc
     8281 atacaaaatg cacgttatcg gcagtcatca ttatttgttg ttgcgcagat ccagcgccac
     8341 gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat
     8401 atccaccgca cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctcaa
     8461 gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gacgatcctg
     8521 caacggacgc accaggtcgt cggcggcatc cattaacgca tagagatcgg tcccatgctg
     8581 ccagcccagt ttatccgcgg cggcaataaa cacttccagc ggtgcgttac ctgcgcccgc
     8641 gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga
     8701 gttcgccacg ccaagactca ggttatggtg agcgtgcatg ccggtttgcg tttctggttt
     8761 caacactgct ttcagggcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc
     8821 agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg cctgcttggc
     8881 gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag
     8941 ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt gcgttgccac
     9001 acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc
     9061 tggcagcaac aacgtcgcga ttttggcgtg cctcaccaca tccgccgccg cttcaatcca
     9121 ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca aaccgtcgcc
     9181 gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa tctggcgaac
     9241 gttttccagc gaatactgat gacgaatggc gtgcataccg tcacgcaatg tgacgtccga
     9301 gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc
     9361 attttttccg ctgtcgccag cgcactggaa gtcataatat cgaggttgcc cgcataggca
     9421 ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ctgttttcag tccggagaat
     9481 tgccccacgc ccggtaaatt gaccggttta tcctgcggga taacttcaaa ctgcacgcgc
     9541 tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag
     9601 gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc
     9661 aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccgcccacc
     9721 acttcaatgg ctcgggaagt ggtttccgta aattcatcga tattggcacg cgtgccaggt
     9781 ccggcagatt tactggcgat agaagcgata atttcggcgt aatgaacacg cgccacgcgt
     9841 gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc
     9901 agttgatcga cgttcgcctc gaggttaacc accggcacgc agtaagggcc gatggcagca
     9961 ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gtaaagcggc atcgtttttc
    10021 acatgcgcgc ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg
    10081 ttcatcagcc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc
    10141 agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg ctgaccgtga
    10201 cgcaaaattt taatcatcag atcggtgcca atgttgccag aaccgataat ggcgacttta
    10261 cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac
    10321 ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc atcgggccta
    10381 atgccccggt aagaatgata tctccggcgc gcagcggttc acccagactg gccattttgc
    10441 gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg
    10501 aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg
    10561 gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat
    10621 cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacttccagc gccggaagta
    10681 cccattcaat ggcgttatac agttcgtcga aggtgatatc agttgcgggt aaatcgcggt
    10741 tcaacaccag tgcgatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatga
    10801 tttcgttatc gccataacac atgtcggcaa ataacgtccc aaaatccggt tgatcaacgc
    10861 ccagttgttg ttgcactttc ggatgcgtca ggcccacttt acgccctacc acgcgacgcc
    10921 cctgcacaac gtcatattgc acatttatgt gctgaatggc gtaggccgct tcagcgtgat
    10981 cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta
    11041 aatccgccgc cagttgctca agagtatgct tcgtcatgac cattccttaa gggcgtgcga
    11101 ggaaattcag caccaattga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac
    11161 agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat
    11221 ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg
    11281 ccagacgtgg gccaaaatcc gggaactgtt tcggattagc ttccaggctc ttaacgaagt
    11341 tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg
    11401 tcaaatcgct ggtatcgaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg
    11461 gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac
    11521 tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa
    11581 gggtgaacgc cacagaacta tggccgccca tcgagttgcc cagcaggtgg attttggcga
    11641 tatccagttg atccaccacg cttttcagga ttcgtgcatt aagatccgat cgtgaaccac
    11701 tattaacgat cgaatcgctt ttgccccaac ccggacaatc cagcaggatc acccgatagc
    11761 ccgcctctac cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc
    11821 cggaaccatg cagcaggaca acggtttcgt cgccttgtcc gcagtcatta aaatggatgc
    11881 gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg
    11941 gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc
    12001 aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc
    12061 aaatgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc
    12121 ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc
    12181 cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagag ttctctgatc
    12241 ctcaacaaac ttctcagcgg cgctaatcac cctttgctga cgcaattcgc gctcactggc
    12301 gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag
    12361 ttcgggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt
    12421 gagagtgctg gtgaaacgtc caatggcttc acccaacatg cgggtacgct ggaaaccggg
    12481 cagcggcgtg gcgacaccgt tgatgaacac aggcagaact ggcaccttat ccagcccacc
    12541 gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc
    12601 aagatcgatc ccgctcttca tgacagcatg cgcacaggcc tccgccagct ccacaggcac
    12661 gggcagctct cctgccgcac tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca
    12721 gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac
    12781 cagttcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc
    12841 atcgagtacc tcttgcgccg ggtcgacata ccccaccaac ggcgagtggg aaagacagtg
    12901 aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca
    12961 gcgtcatcac cgacgccagt ttattcagcg tattgcccag ggtttgcgga atggcggtgg
    13021 cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc
    13081 tacgcagacg accttgcgta tcgcccacgt gtagcacgcc gtcgtggtta tcctgtgcgg
    13141 tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct
    13201 ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt
    13261 tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg actttcggct
    13321 gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc
    13381 cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca
    13441 gataattcaa cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag
    13501 ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt
    13561 cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt
    13621 tccaggcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg
    13681 gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacggaaa cgttgcgcca
    13741 gtcgcgcgtt atgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca
    13801 gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgctct tcggtttctc
    13861 ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg
    13921 ggcgcaccgg atcgcaacac aaatagatat gcggcgtact taacggatcg ttggcgatat
    13981 ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac
    14041 gaacaaaact tgccccacca tcacaggcca ccagccactg ggctttgatc gtttcccgct
    14101 gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca
    14161 gctcgcggga aaacaagcag cgcacattcg gaaaacgcga caacccttcc agcatcaccg
    14221 catcgacctg tggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg
    14281 gctgaatatc agcaaaacag cggccttttg gggtgagaaa acgcatcgcg tgccacggtg
    14341 tagtgtgcgg cagaacattc tcgaccaggc cgaccgactg catggtgcgc agcgcctcgt
    14401 catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca
    14461 cgtcaatgcc catctgaccg agatagttcg ccatcatcag cccaaccgga ccggcaccag
    14521 cgatcgccac ctgaacgcta tggttaacag caggctggat gtcagggtgt tgtattgcca
    14581 tttctgcaca acgttgtttt aactatagaa atgtcaatta atatgcagaa caatgagcag
    14641 acggaataca aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat
    14701 aaacttgatg gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc
    14761 actgtgcggc gactgctgga gacgctgcag gaagagggat atgtccgccg tagcccctcc
    14821 gatgacagtt ttcgtctgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa
    14881 cagtggattt ctgcactggc ggccccgctg ctgggcgatc tgttgcgcga agtggtatga
    14941 ccaacagatg tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac cacccaccgt
    15001 ttcagccgct tatcctttca ccgggcaatg gtcgggcgac gtttaccgct cctgaaaacc
    15061 gcctcgggcc tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgaa
    15121 atgttagccg cccgccccgg tgatgactat caactggcac gagaaccgtt aaagctgcaa
    15181 gccattctgg cgcgcgcgcg caaagagggt tacggacaga actaccgcgg ctgggatcag
    15241 gaggagaaga tcgcctctat cgccgtaccg ctgcgcagtg aacaacgggt gattggctgt
    15301 ctgaatctgg tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt
    15361 ccggcgctac aacgggtagc aaaacagatc gaagaagggg ttgaatcgca ggctattctg
    15421 gtggccggac ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt
    15481 tcgtggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
    15541 agtaacgcta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttctcgcgt
    15601 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
    15661 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
    15721 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    15781 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    15841 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    15901 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
    15961 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    16021 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    16081 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
    16141 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    16201 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    16261 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    16321 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
    16381 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    16441 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    16501 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    16561 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    16621 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    16681 cggctcgtat gttgtgtgaa attgtgagcg gataacaatt tcacacagga aacagctatg
    16741 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16801 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    16861 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    16921 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    16981 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    17041 accaacgtga cctatcccat tacggtcaat ccgccttttg ttcccgcgga gaatccgacg
    17101 ggttgttact cgctcacatt taatattgat gaaagctggc tacaggaagg ccagacgcga
    17161 attatttttg atggtgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    17221 tacggccagg acagccgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    17281 aaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gtagttatct ggaagatcag
    17341 gatatgtggc gaatgagcgg cattttccgt gacgtctcgt tgctgcacaa accgagcaca
    17401 caaatcagcg atttccatgt tgccactcac tttaatgatg atttcagccg cgcggtactg
    17461 gaggcagaag ttcagatgta cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg
    17521 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    17581 gagcgtggtg gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg
    17641 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgacgacggc
    17701 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    17761 ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca cgagcatcat
    17821 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    17881 cagaacaact ttaacgccgt gcgttgttcg cattatccga accatccgct gtggtacacg
    17941 ctgtgcgacc gcttcggctt gtacgtggtg gatgaagcca atattgaaac ccacggcatg
    18001 gtgccaatga atcgtctgac cgatgatccg cgctggttac ccgcgatgag cgaacgcgta
    18061 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    18121 gagtcaggcc acggcgctaa tcacgacgca ctctatcgct ggatcaaatc tgtcgatcca
    18181 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    18241 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    18301 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    18361 gcgatgggta acagtcttgg cggcttcgct aaatactggc aggcgtttcg tcagtacccc
    18421 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    18481 aatggcaatc cgtggtcggc ttacggcggt gattttggcg atacaccgaa cgatcgccag
    18541 ttctgcatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacagaagca
    18601 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    18661 gaatacctgt tccgtcatag cgataacgag cttctgcact ggtcggtggc gttggatggc
    18721 aagccgctgg caagcggtga aatgcctctg gatgttgctc cacaagataa acagttgatt
    18781 gaactgcctg aactaccgca gccggagagc gccggacaac tctggcttac ggtacacgta
    18841 gtgcaaccga acgcgaccgc atggtcagaa gccggacaca tcagcgcctg gcagcagtgg
    18901 cgtctggcgg aaaacctcag cgtgacactc cccgccgcgc cccacgccat cccgcaactg
    18961 accaccagcg aaacggattt ttgcatcgag ctgggtaata aacgttggca atttaaccgc
    19021 cagtcaggct ttctttcaca gatgtggatt ggcgctgaaa aacaactgct gacgccgctg
    19081 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    19141 attgacccga acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    19201 gcgttgttgc agtgcacggc agatacactt gccgacgcgg tgctgattac gaccgctcac
    19261 gcgtggcagc atcagggaaa aaccttattt atcagccgga aaacctaccg gattgatggt
    19321 agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggct
    19381 cggattggcc tgacctgcca gctggcgcag gtagcagaac gggtaaactg gctcggatta
    19441 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    19501 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    19561 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caatatcagt
    19621 cgctacagcc aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    19681 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    19741 ccgtcagttt cagcggaatt ccagcttagc gccggtagct accattacca gttggtctgg
    19801 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
    19861 attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac
    19921 ttttttatca tgggggccta cttcccgttt ttcccgattt ggctacatga catcaaccat
    19981 atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc
    20041 caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt
    20101 attaccggca tgttagtgat gtttgcgc
//
LOCUS       DAZOYS010000064        19735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOYS010000064 DAZOYS010000000
VERSION     DAZOYS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.064"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..597)
                     /locus_tag="ACHUN9_001992"
     CDS             complement(<1..597)
                     /locus_tag="ACHUN9_001992"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR0695392.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQ"
     gene            complement(684..2981)
                     /locus_tag="ACHUN9_001993"
     CDS             complement(684..2981)
                     /locus_tag="ACHUN9_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR0695393.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(2971..3246)
                     /locus_tag="ACHUN9_001994"
     CDS             complement(2971..3246)
                     /locus_tag="ACHUN9_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR0695394.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3243..3467)
                     /locus_tag="ACHUN9_001995"
     CDS             complement(3243..3467)
                     /locus_tag="ACHUN9_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR0695395.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3470..3769)
                     /locus_tag="ACHUN9_001996"
     CDS             complement(3470..3769)
                     /locus_tag="ACHUN9_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR0695396.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3769..3993)
                     /locus_tag="ACHUN9_001997"
     CDS             complement(3769..3993)
                     /locus_tag="ACHUN9_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR0695397.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4057..4557)
                     /locus_tag="ACHUN9_001998"
     CDS             complement(4057..4557)
                     /locus_tag="ACHUN9_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR0695398.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4727..4999)
                     /locus_tag="ACHUN9_001999"
     CDS             complement(4727..4999)
                     /locus_tag="ACHUN9_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR0695399.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5136..5429
                     /locus_tag="ACHUN9_002000"
     CDS             5136..5429
                     /locus_tag="ACHUN9_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0695400.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5499..6479
                     /locus_tag="ACHUN9_002001"
     CDS             5499..6479
                     /locus_tag="ACHUN9_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0695401.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6665..7165)
                     /gene="cpxP"
                     /locus_tag="ACHUN9_002002"
     CDS             complement(6665..7165)
                     /gene="cpxP"
                     /locus_tag="ACHUN9_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0695402.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7315..8013
                     /gene="cpxR"
                     /locus_tag="ACHUN9_002003"
     CDS             7315..8013
                     /gene="cpxR"
                     /locus_tag="ACHUN9_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0695403.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8010..9383
                     /gene="cpxA"
                     /locus_tag="ACHUN9_002004"
     CDS             8010..9383
                     /gene="cpxA"
                     /locus_tag="ACHUN9_002004"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0695404.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9489..10163)
                     /gene="yiiM"
                     /locus_tag="ACHUN9_002005"
     CDS             complement(9489..10163)
                     /gene="yiiM"
                     /locus_tag="ACHUN9_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0695405.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10312..11295)
                     /gene="kdgT"
                     /locus_tag="ACHUN9_002006"
     CDS             complement(10312..11295)
                     /gene="kdgT"
                     /locus_tag="ACHUN9_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0695406.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11555..12175)
                     /gene="sodA"
                     /locus_tag="ACHUN9_002007"
     CDS             complement(11555..12175)
                     /gene="sodA"
                     /locus_tag="ACHUN9_002007"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0695407.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12460..13494
                     /gene="rhaT"
                     /locus_tag="ACHUN9_002008"
     CDS             12460..13494
                     /gene="rhaT"
                     /locus_tag="ACHUN9_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0695408.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13491..14339)
                     /gene="rhaR"
                     /locus_tag="ACHUN9_002009"
     CDS             complement(13491..14339)
                     /gene="rhaR"
                     /locus_tag="ACHUN9_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR0695409.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14413..15249)
                     /gene="rhaS"
                     /locus_tag="ACHUN9_002010"
     CDS             complement(14413..15249)
                     /gene="rhaS"
                     /locus_tag="ACHUN9_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0695410.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15537..17006
                     /gene="rhaB"
                     /locus_tag="ACHUN9_002011"
     CDS             15537..17006
                     /gene="rhaB"
                     /locus_tag="ACHUN9_002011"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0695411.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17003..18262
                     /gene="rhaA"
                     /locus_tag="ACHUN9_002012"
     CDS             17003..18262
                     /gene="rhaA"
                     /locus_tag="ACHUN9_002012"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0695412.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18504..19328
                     /gene="rhaD"
                     /locus_tag="ACHUN9_002013"
     CDS             18504..19328
                     /gene="rhaD"
                     /locus_tag="ACHUN9_002013"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0695413.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19338..19652
                     /gene="rhaM"
                     /locus_tag="ACHUN9_002014"
     CDS             19338..19652
                     /gene="rhaM"
                     /locus_tag="ACHUN9_002014"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0695414.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4918 a         5102 c         4910 g         4805 t
ORIGIN      
        1 ttggccatct tctaatggag gaagatttcc tattgcatct ctaactgttt tcaaaataac
       61 atcgtcacgt tttactaact caatcgcccc caattttgag gccaacaata catgacggga
      121 acggttttgt gggattccat attcgacaca atcaatctta gacgcccaaa cataatagcc
      181 caactttaac aatgaattat aaaaatcatt ataaacttta tgtttagtta catcaggaac
      241 gttctccatt gttacgattt ctggagatac ttcgcgaatt aaacgttcga actcataaag
      301 aagaggccat tttttatcat cagccttatc ttttccttga gtatattttg aaaaaggttg
      361 gcaaggtgcg catcctgcca atacttttac agacgcatca ccataaagcc tcagcagttc
      421 ctcttttgtt actttagcga tatcttgctc aataaactta gatttattgt ttctttcata
      481 gggaaaccta cattccccct caaggtcaat gcctgcaacg acatcaagcc ccgcaagctg
      541 caaaccatga gttaatccgc cagcaccaca aaaaaggtct atcaccttta cagtcatcac
      601 tcactccaaa tctatacgat actcagtata catccctttc tacaaaatct cgaggcctaa
      661 cttataaact catatttatt gagttaacgc ttcactgagt ttgactctaa tactgttgag
      721 aatcccgacc actcatcagc gagcgggtaa gtgaattttt tcccgtcata atttacggtt
      781 gccccacgcg ccagcacctc aagctcccat cgctgcggcc tgataccgtt ctgagcaagg
      841 tcaacgcgga tacgggtaat ttgcattcgt tccgaccggg tcagtctggc cgatggcgct
      901 atttcatgcg gttttaacgg gcttccgttt ctttgctgac ggtttggtct tctcagtccg
      961 tgttttaatg cacctctgag cgccctcaca acctccgggt cattccattc gataacaccg
     1021 tcatctacca gattaagcac tgctgcggcg tgctcagaag gtgtgggagc cggtaacgaa
     1081 gtatcaccac cagtgagctt tccacagtta ttgacaggac tccgaggcgc ggcgatgccg
     1141 ctttttaaag tcaaaggctc aacgaccggc actttcggaa caatgcgcca gtccgtcgtt
     1201 ctggtgatat gaatatgacg cgcgccgaga tgcggcgcgt aaatgccgac cactctctcg
     1261 acttcttcct cgtactcgtt aacttcatcc gacggactac gggcgaccct gacagtctga
     1321 caatcgcgcg ggacatttgc cccaccctgc gcgctgatat acagcgcaaa atcaccactg
     1381 tctgcggcgg cgcgtgcagc ctcgacgcgc tcgtcaaact catcagcaat gctgacgccg
     1441 cgaggcaatt tgcgtagttc acggtaagcc cccattgtcg gcaggccaac cgttttaaat
     1501 tgcggaatgc gccacgttga cgcccatgcg gtaacagccg cggcagtatc tttcagcggt
     1561 ctgccgctat cgttatcgag ctgcccatcc agtgcatagc cgtcgatatt ttttgagatg
     1621 tatttcgcga tataccccgc agcaccgccc cgattaagat gttttgcctg aaaacggttt
     1681 cgcgcagctc ctctttcgtc gccatcctct ttgagcgcgt agcgacgcat gatttcgata
     1741 atctggttac gctggcgtgg attacaaaaa agcatcatat gccagtgcgg cgttccgtcg
     1801 tggtgtggct cgacgactcg caaaccgtag acctgtaaat cattatcctt gaatgccgtg
     1861 cgcatcaggc tccagatacg gcagagataa cgctgcgcat cctttggatt aaatgcctca
     1921 tcgttccagc cgtgatttag ctggacggtt ttactttcgc cttttccaac ctgacgtgtc
     1981 gggtgatact ttgacggcgc ggtcagcgtg ataaacatcc ccacatcacc ttctgcggcg
     2041 gcgtaacgct caataccggc aatggtgttc atcagctcca tccggcgaat ttcaggatta
     2101 gaaatactgc ccatcacctt actgataagg tcgatgcgct cgcctgtttc cctgttttcg
     2161 agatcacacg atttaagaaa ttccagattt gcctggcggc gtgcacgcac atcacgaatg
     2221 gcatgtttac tggcataagg agaacggtct ttattgacct ccccgacagc aatcagtaac
     2281 gcctcatgcc agcgcatacg ctggccttta agctgactaa tccaccactc atcgttaaac
     2341 agacgggcaa tggcagaata tgcctgcctc gtggtcatct gtcctttacg gtattttttc
     2401 cagtagagag gggaaatatt gaaagcacgt gcagcgccag caacatgacc atagaggtga
     2461 gcctgcgcct catccgtaaa cagcgattct ttttcgccat gcgcatccac ccaggcatcg
     2521 ctgagttcct catacatcat gaaaagctgc gatgagatac gggcggcaaa ctttttcagc
     2581 tccttgtcat tcattcccgg caggcgcgca tagtggtcac gctcagccag aaacagcatc
     2641 gacgcgtcgg tgttcatttc atggcgctga ttcacacgct caatgcgcgg ccataaacga
     2701 cgctgaaaag tggatgtgag gaaataaaac ccgtgcaccg ggcttttatt gcgccggatg
     2761 tagtcatagc gtgacgtaaa cagcgagcgc aaaaagtaag gcaggcggtt aatcgtggat
     2821 aaaacacctt gcacctgacg catctcgtca cgtgtaaggg gtctttcgcg cccgacagcc
     2881 tcgcgtggcg cgttccatgc ataagcaccg gtaaacgcct taccggtgcc tgcagcaaat
     2941 gctgacggag ggacaaaacg cccggaggct ttaacggcca tatgagccaa aagcctctga
     3001 acaacgcttg ctgagttgct caacctgcgc gtttaaatca gcaaaagact ttgcgcttcc
     3061 ggtcagaata tcgtgatgca tcaggccgga aacgagctgg cttaatttcg ggtaataacc
     3121 aaccaccgcc agccattcct gaccggcgtt tttaccgctt tccgctctct ttttctcgtg
     3181 gagaataaac tggaagctgt cactggtaac gacataacgt tcgccaattt caatacgaat
     3241 actcatgccg ttctccggta atgtttgttt tttgcttcaa agactgactg acaggaaaca
     3301 caacgcgtgg ctgacggata agccgcacga cgggcagcag gaattggcgc gtcacactct
     3361 tcgcaaacca gcgcagaagc accgcaatgt tttacccttg ccgcgttaat ctggcgctcc
     3421 agtaattcag cctgttgttc ctgaataaaa tctatgttgt ccggcattat cagctccttt
     3481 tatcgttaag tttcctggat acatcagcgc aataactggc aagttctgtc gttaattttg
     3541 tcagttcatc cactgaggaa atttgcttgt ggaatacagc gcgtttaaca agtaaattga
     3601 ccacatcaga caggaggttt aattcattct gataaatcgc gataacagat tcagtgatgt
     3661 cgcgtttttc tttatcaaga caaagttgaa taagagacaa gtcgccattt ttcataacgg
     3721 cgatttttaa ggcgttattc agtaatacaa ctgaatgaga acaggacatc aaagcacctc
     3781 cccgcgagac aatccgatat tgtgaaattt ttccgactcc tgactgagca gctcgactat
     3841 ctccacgcgg gataactccg cctttgtgat atggcgaatc atggcgtcaa gatgagaaga
     3901 aaagcgcgtc gcagcgtcgg cctgtgcttc ggttctggcc tgttgcagca gtaatgcgta
     3961 tttaccgcac tgattttcag aaactgtatg catgactttc tccaggcaaa aagaagcccc
     4021 gcacaattaa gtgcgttaaa aactctggtt aattatttaa tgcaaatatt gctctggttt
     4081 taccgacgtc agaattgtcg gtgcatactc aaacagactg aatagttcac gtaatgcacg
     4141 gaataaagca tcacgccagt aacatgactc ttcattaatt cgccagtatg gctgattgaa
     4201 ttctttttca gtcaatccgg catgcataaa taaagtacga cgctgactga ctgttaaaaa
     4261 actaatatat gcatactcac ttgcgccaac ctgacggcgt tttgagaatg ccccacgcaa
     4321 ttcatcaatt gcacaaacca gccgttcacg ttcgacgtcg ttcatttctt caaaacgcat
     4381 cgttgcgtga cgttgtttta actgcgcatg aaagcaaact gttagccgtt cgcgctccat
     4441 catctgatta taataatcac atgtatcctg ccaacgaggg acggcaagat gcttaccaat
     4501 tatccggcgc atagctgctg gctgtttttc gacgagattg agcgtcatca ctgtcatttc
     4561 cagaccctcc ggcttttcag aaaggtcaga gcctttttta acggactctg ttttttggtg
     4621 cggataatga ttcccttgcg acccttcccg tgggtgatgg tgaagtcaat cgccctaggg
     4681 ctttcgttac gcaataactg agcaatacaa cgcggctcat tcataatcac aaccccatcc
     4741 acaaaagcca tgcatcacgc tgttcaaccg gtcggttata aaacgcctca cgtacagcgc
     4801 gattaaactc aggaatgaaa acccattttt caccggcacg agccttcggt ttgcaaggat
     4861 cacgcaattc aataattggt aatttatttg ccttcaccat ttcactgacg gctgtctttg
     4921 gcttccctaa taaatcagca aatttatcca catgaaccgc atcaagcgga tactgaatca
     4981 cataattttc agcgtccata tatggtaccc tcataggatc cagccctttc taaaccactc
     5041 aaaaccgttt agacgctggt ttattctcaa atcaatggaa cctatatagg ttccagtttt
     5101 gagggaattt agtccctata taggcaccat gtcaaatgaa attaagcgaa aagattaagg
     5161 ccttgcgtga ggctgaaggg ctaagccaat caaaattctg tgaaatcata gagttaccgc
     5221 taagcacact taaaaaatat gaaggaggaa actttgaacc cggtggcaca gctttgctaa
     5281 aaatcactat gcatcccaca ttccaaaaat atgctctatg gcttatgaca gataaaaccg
     5341 cgccggacgc aggacaaatc gcaccggctc tcgcgcacat tgggccagag tcaacagagt
     5401 ccaaccactc cgcgaaaagg attggctaac tctatataaa gattacattt tcaccatttg
     5461 ctaccaagat ggtgaataca gcgccggagg gctttcttat ggcaattaag aagctcgatg
     5521 atggtcgcta tgaagtggac attagacctc gcggtcgcga cggaaaacgc atccgcagga
     5581 aattcgaaag aaaagctgaa gcactagcat ttgagcgata cacaatcgcc aatgccagtc
     5641 agaaagaatg gggaggccag cgagcagacc gccggacttt gagtgagttg ctggacatct
     5701 ggtggaaata tcacgggcaa aaccacgagc atggaacaaa agagtttaat catctactca
     5761 aaaccatcag cggcataggt gatataccag tgagcaggat gagcaaaagg gctttgatgg
     5821 attatcgttc catgcgacta cgtgatggta tcagtgccgc aacgataaac cgtgacatgt
     5881 acagattatc cggcatgttc acaaaattaa ttcaattgga tgaattttcc gggcaacacc
     5941 caattcacgg actgccgcca ctggcggagg ccaaccctga aatgacgttc ctggaaaaag
     6001 cagaaatcga aaaactgtta aatgttttgg ctggtgatga cttacttgtc gcgcttttat
     6061 gtctgagcac tggaggaaga tggacgaaag ttgccacgct aaaaccagca cagattacaa
     6121 attgcagggt taccttcctg aaaaccaaaa acggtaaaaa gcgaaccgtg ccgatttctg
     6181 aggaactgga gaaaaaagtt aaagaggagg ccagcgccaa attattcaaa gttgattatg
     6241 agaaattttg cgggatttta cgcagagtga aacctgatat accacccaat caggcaaccc
     6301 acatcctgcg gcatacattc gcaagccatt tcatgatgaa tgggggcaat ataatcgcac
     6361 tgcaacagat tctgggacat gcgagcattc agcagacgat ggcctatgcg caccttgcgc
     6421 ctgactatct gcaaaatgcc gtcgctctga atccactaaa aggcggagtg acgttataaa
     6481 tttccccctc tgagtgtcca catagtgtcc acactcaaga aattttgaaa ccgctccaaa
     6541 cccttacagc tttcgataag taactgtttt tacttttgat tgcatgtaag tatttgataa
     6601 aaaaaacccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg
     6661 tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc
     6721 attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt
     6781 gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac
     6841 gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat
     6901 tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt
     6961 cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta
     7021 tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt
     7081 tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca
     7141 tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac
     7201 gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag
     7261 tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat
     7321 aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag
     7381 atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac
     7441 gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca
     7501 ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt
     7561 gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt
     7621 aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag
     7681 caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat
     7741 ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt
     7801 accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc
     7861 caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat gcacatttcc
     7921 aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt
     7981 ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg
     8041 ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg
     8101 attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc
     8161 agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc
     8221 tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg
     8281 aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc
     8341 aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc
     8401 cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca
     8461 gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg ctgattgtca
     8521 ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc
     8581 gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac
     8641 tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg
     8701 cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc
     8761 gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa
     8821 gcaaggaact cgagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc
     8881 tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca
     8941 accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt
     9001 cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgccctgg
     9061 aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag
     9121 tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg
     9181 ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg
     9241 atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc
     9301 atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt
     9361 tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg
     9421 catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac
     9481 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
     9541 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
     9601 tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
     9661 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
     9721 acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata
     9781 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
     9841 tctccccagc ggaaaatatc gcccatatga acattacttt ccgtgagacc gtcggttgag
     9901 agattttcgc caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcacc
     9961 cagtagagat agtgctcacg aggataatga cacagcgctc tgtctggccc gccgtgaacc
    10021 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
    10081 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
    10141 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
    10201 tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag
    10261 caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg tttatttcac
    10321 cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc agatagaggt
    10381 aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg aagttgctgc
    10441 cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc
    10501 ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt
    10561 atcggcgata atcaacggaa taccggtcac gataattact gccacaccca acaggatccc
    10621 cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa
    10681 gaatggaatc agcgtttgca ctgctttgct gaaaaattct cgtaattcag ggtcaagatt
    10741 ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa
    10801 cgaggcaatc ccggcagtgc ccagaataat catcgtcatg agcggcccgg actccaacga
    10861 catcaacaca aatgccccag cttcttcttt cgtaccgtac tgctgcatga tggaagcgta
    10921 aagtccgccg ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa
    10981 gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg caaccaccca
    11041 cgcgacggca attttagtta ccaccagcgt accggattta cgcagtaccg ttcccgtcgc
    11101 gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg gcaccgtacc
    11161 ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg
    11221 gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgggattt tctcaatcga
    11281 gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat gcgtaattcc
    11341 tcaatggagc atcagtgttg atggaaacga agatagacat tcatttaatc aataaaagtg
    11401 atcaaaacca catcaattga aacgctgttt tatttttatc ggatcattgt ttttattttg
    11461 aaattgatca caaaaaaacc accgccgttg gcgatggttc attttttaag ctgatatgcg
    11521 gcctatacgc ctcattgcag caggcggcaa atgattattt tttcgccgca aaacgtgccg
    11581 ctgcttcgtc ccagttcacc acgttccaga actctttaat gtagtccgga cggcggttct
    11641 ggaatttcag gtagtaagca tgttcccaca catccaggcc cagaatcggg aagccggaag
    11701 cgccagaaat agcttcaccc atcagcggag aatcctggtt agcagtagaa accaccgcca
    11761 gtttatcgcc tttcagcacc agccatgccc agccggaacc aaagcgggaa gctgccgctt
    11821 tttcaaattc tgctttgaag ttatcaacgg agccgaagtc acgttcgata gccgctttca
    11881 ggtcaccctg cagggtggtg ccttttttca gacctttcca gaacaggctg tggttagcgt
    11941 gaccgccagc gttgttgcgc agtacggttt tcttgtctgc tggcagctgg tccagtttgg
    12001 tgatcagctc ttcaaccggc aggttggcaa attctggcag gctttccagc gccgcgttgg
    12061 cgttgtttac gtaggtctga tggtgtttgg tgtggtggat ttccatggtc tgcttatcga
    12121 agtgcggttc cagggcatcg taagcatacg gcagggatgg cagggtatag ctcatattca
    12181 tctccagtat tgtcgggcgg ccgattgtta atgccgcgta agcagttggt tcattatagt
    12241 taattaaatg atattgaaaa tgattatcaa tgccgtactt ttcgtaaggg tatggttttg
    12301 caggaaaatg cccgagatgt gaagcaaatc acccacttaa tgccgtgatt gccagtaaat
    12361 cgacaacggc ggcaacaggc gaaaggttaa tcgacagcac gatttttaca ctcatctcgt
    12421 cggagatgtg aagcgacgaa aaatgatgag gataagaaga tgagtaacgc gattacgatg
    12481 gggatatttt ggcatttgat cggcgcggcc agtgcagcct gtttttacgc tccgttcaaa
    12541 aaagtaaaaa aatggtcatg ggaaaccatg tggtcagtcg gtgggattgt ttcgtggatt
    12601 attctgccgt ggaccatcag cgccctgtta ctaccggatt tctgggcgta ttacagctcg
    12661 tttagtctct ctacgctgct gcctgttttt ctgttcggcg ctatgtgggg gatcggtaat
    12721 atcaactatg gcctgaccat gcgttatctc ggcatgtcga tgggaattgg catcgccatt
    12781 ggcattacgt tgattgtcgg tacgctgatg acgccaatta tcaacggcaa tttcgatgtg
    12841 ctgattaaca ccgaaggcgg acgcatgacg ttgctcggcg ttctggtggc gctgattggc
    12901 gtagggattg taactcgagc cgggcagttg aaagagcgca agatgggcat taaagccgaa
    12961 gagttcaatc tgaaaaaagg gctggtgctg gcggtgatgt gcggcatttt ctctgccggg
    13021 atgtcctttg cgatgaacgc cgcaaaaccg atgcatgaag ccgctgccgc acttggcgtc
    13081 gatccactgt atgtcgctct gccaagctat gttgtcatca tgggcggcgg cgcgatcatt
    13141 aacctcggtt tctgttttat tcgtctggca aaagtgaagg atttgtcgct aaaagccgac
    13201 ttctcgctgg caaaaccgct gatcattcac aatgtgttac tctcggcact gggcgggttg
    13261 atgtggtatc tgcaattctt tttctatgcc tggggccacg cccgcattcc ggcgcagtat
    13321 gactacatca gttggatgct gcatatgagt ttctatgtat tgtgcggcgg tatcgtcggg
    13381 ctggtgctga aagagtggaa caatacagga cgccgtccgg taacggtgtt gagcctcggt
    13441 tgtgtggtga ttattgtcgc cgctaacatc gtcggcatcg gcatggcgaa ttaatctttc
    13501 tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt aaacaccacc
    13561 gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat taacaggcgg
    13621 ctatgctgga gaagatattg cgcatgacac actctgacct gtcgcagata ttgattgatg
    13681 gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca cgatgcctca
    13741 tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccaaccg ggtaatcagc
    13801 ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta acgatggcga
    13861 ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc aaacgacaca
    13921 tgctgactac tttcatgctc aagctgaccg ataacctgcc gcgcctgcgc catccccacg
    13981 ctacctaagc gccagtgtgg ttgccctgcg ctggcgctaa atcccggaat cgctccctgc
    14041 cagtcaagat tcagcttcag ccgctccggg caataaataa tattctgcaa aaccagatcg
    14101 ttaacggaag cgtaggagtg tttatcgtca gcatgaatat aaaagagatc gccacgggta
    14161 atgcgataag gacgatcgtt gagtacatgc agaccattac cgcgccagac aatcaccagc
    14221 tcacaaaaat catgtgtatg ttcggcaaag acatcttgcg gataacggtc agccacagcg
    14281 actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg atgcgccacc
    14341 gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac aaatacgttg
    14401 agaagattcg cgttattgca gaaagccatc ccgtccctgg cgaatatcac gcggtgacca
    14461 gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc cacagcgata
    14521 ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt tcatcagtcg
    14581 caggcggttc aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct gccgatgtag
    14641 cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct catcggcaaa
    14701 atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt ccaggttctc
    14761 ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct cgcgactggc
    14821 ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca gctgtcgcac
    14881 ctgctgcaat acgctgtggt taacgcgcca gtgagacgga tactgcccat ccagctcttg
    14941 tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc gatacagcac
    15001 attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgag cgcgtacgaa
    15061 acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa tacccgtgcc
    15121 atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat ccgcctgcgg
    15181 gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca cactatgtaa
    15241 tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca cgaggtcagg
    15301 ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt caagatacag
    15361 cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aatgaagcaa attgtgaaca
    15421 tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt aacgagaagg
    15481 tcacgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat cacattatga
    15541 cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg atgctggcgc
    15601 gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt aacaatggcc
    15661 tgcatagcca gaacggttat gtcacctgga atgtggatag cctggaaagt gccattcgcc
    15721 ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg attgatacct
    15781 ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg cccgttgctt
    15841 atcgcgatag ccgctccaat ggcctaatgg cgcaggcaca gcaacaactc ggcaaacgcg
    15901 atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat caactgcgtg
    15961 cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg ctgatgccgg
    16021 attacttcag ctatcgcctg accggcaaga tgaactggga gtacaccaac gccacgacca
    16081 cgcaactggt caatatcaat agcgacgact gggacgagtc gctactggcg tggagcgggg
    16141 ctaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg cactggattt
    16201 gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc gccagcgcgg
    16261 ttatcgcctc gccgttaaac ggttcacgcg ctgcttatct ctcttctggc acctggtcat
    16321 tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca gccaacataa
    16381 ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg ggcttatggc
    16441 tgcttcagcg agtgcttcag gagcggcaaa tcaacgatct cccggcgctt atcgccgcga
    16501 cacaggcact tccggcctgc cgcttcatca tcaatcccaa tgacgatcgc tttattaatc
    16561 ctgaggcgat gtgcagcgaa attcaggctg cgtgtcggga aatggcgcaa ccgctcccgg
    16621 aaagtgatgc tgaactggcg cgctgcattt tcgacagtct ggcgctgctg tatgccgatg
    16681 tgttgcatga gctggcgcag ctacgcggtg aagatttctc gcaactgcat attgtcggcg
    16741 gcggctgcca gaacacgctg ctcaaccagc tatgtgccga tgcctgcggt attcgggtga
    16801 tcgccgggcc tgttgaagcc tcgacgctcg gcaatatcgg catccagtta atgacgctgg
    16861 atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg aatctgacca
    16921 cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagctt cactcgacac
    16981 gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg agctggcgaa
    17041 acagcgtttc gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac ttgatcgttt
    17101 acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa acccggaagg
    17161 ttcgctgacc ggtgggattc aggctactgg taattatccg ggcaaagcgc gtaatgccag
    17221 tgagctacga gccgatctgg aacaggctat gcggctgatt ccggggccga aacggcttaa
    17281 tttacatgcc atctatctgg aatcagacac gccagtctcg cgcgaccaga tcaaacctga
    17341 gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg attttaaccc
    17401 ctcctgtttc tcgcatccgc taagcgccga tggctttacg ctctcgcatc ccgacgacag
    17461 cattcgccag ttctggattg atcactgcaa agccagccgt cgcgtttcgg cctattttgg
    17521 cgagcaactc ggcacaccat cggtgatgaa catctggatc ccggatggta tgaaagatat
    17581 caccgttgac cgtctcgccc cgcgtcagcg tctgctggca gcactggatg aggtgatcag
    17641 cgagaagctg gatccggcgc accatatcga cgccgttgag agcaaattgt ttggcattgg
    17701 cgcagagagc tacacggttg gctccaatga gttttacatg gggtatgcca ccagccgcca
    17761 gactgcgctg tgcctggacg ccgggcattt ccacccgact gaagtgattt ccgacaagat
    17821 ttccgccgcc atgctgtatg taccgcagtt gctgctgcac gtcagccgtc cggttcgctg
    17881 ggacagcgat cacgtggtgc tgctggatga tgaaacccag gcgattgcca gtgagattgt
    17941 tcgtcacgat ctgttcgacc gggtgcatat cggcctcgac ttctttgatg cctctatcaa
    18001 ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc tgcgtgcgtt
    18061 gctggaacct accgctgagc tgcgcaagct ggaagcggcg ggcgattaca ctgcgcgtct
    18121 ggcgctactg gaagaacaga aatcgttgcc gtggcaggcg gtctgggaaa tgtattgcca
    18181 acgtcacgat acgccagcag gtagcgaatg gctggagagc gtgcgggctt atgagaaagc
    18241 gattttgagc cagcgtgggt aaacactgcc ggatgcggcg cgagcgcctt atccggccta
    18301 caggtcgaat gaccatgcgt gctgccaccc tcaccccggc cctctccctg aaagggcgag
    18361 ggagaaaaac atagcctcgt gcggcaacgg agctgaacac cgctccggac agtcccctcg
    18421 cccctacggg gagagggtta gggtgagggg aacaagccgc accatgtcta aacaaggcaa
    18481 cctaatttac aggaacacag aacatgcaaa acattaccca gtcctggttt gtccagggaa
    18541 tgatcaaagc caccaccgat gcctggctga aaggctggga cgagcgcaat ggtggtaacc
    18601 tgacgctgcg cctggatgac gccgatatcg cgccttatca cggcaacttt catgcacaac
    18661 cgcgctatat tcccctcagc caacccatgc ctttactggc aaatacgccg tttattgtca
    18721 ccggctccgg caaattcttc cgtaacgtcc agcttgatcc tgcagctaac ttaggcgtcg
    18781 taaaagtcga cagcgacggc gcgggctacc atattctctg ggggttaacc aacgaagccg
    18841 ttccaacttc cgaacttccg gctcacttcc tttcccactg cgagcgcatt aaagccacca
    18901 acggtaaaga tcgggtgatc atgcactgcc acgccaccaa cctgatcgcc ctcacctatg
    18961 tacttgaaaa cgacaccgcg gtctttactc gccaactgtg ggaaggcagc accgagtgtc
    19021 tggtggtatt cccggatggc gttggcattc tgccgtggat ggtgcccggc acggacgaaa
    19081 tcggccaggc gaccgcacaa gagatgcaaa aacattcgct ggtgttgtgg cccttccacg
    19141 gcgtcttcgg cagcgggtcg acgttggatg aaacattcgg tttaatcgac accgcagaaa
    19201 aatcagcaca agtattagtg aaggtttatt cgatgggcgg catgaaacag accatcagcc
    19261 gtgaagagtt gatagcgctc ggcaagcgct tcggcgttac accactagct agtgcgctgg
    19321 cgctgtaagg agcaaacatg atccgcaaag cctttgtcat gcaggttaac cccgacgccc
    19381 acgaagagta tcagcgtcgg cataacccca tctggccgga actggaagca gtgttgaaat
    19441 ctcacggtgc gcataactac gccatttacc tcgacaaagc gcgtaatctg ctgtttgcca
    19501 cggtagagat tgaatctgaa gaacggtgga atgcggtagc cagcactgat gtttgccaac
    19561 gttggtggaa atatatgacc gatgttatgc ccgctaaccc ggataacagc ccggtgagta
    19621 gcgagctgca agaagtgttt tacctgccgt aattgcccaa tgtgccagag atgcctccgg
    19681 cacaatattt taaatttcga tttgaaaaat taaacatatt acatctcccg gtaag
//
LOCUS       DAZOYS010000065        19530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOYS010000065 DAZOYS010000000
VERSION     DAZOYS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.065"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUN9_002015"
     CDS             <1..245
                     /locus_tag="ACHUN9_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0695415.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUN9_002016"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUN9_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0695416.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUN9_002017"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUN9_002017"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0695417.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNNNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUN9_002018"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUN9_002018"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0695418.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUN9_002019"
     CDS             3423..4061
                     /locus_tag="ACHUN9_002019"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0695419.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUN9_002020"
     CDS             4066..4842
                     /locus_tag="ACHUN9_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0695420.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUN9_002021"
     CDS             4931..6295
                     /locus_tag="ACHUN9_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0695421.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUN9_002022"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUN9_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0695422.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUN9_002023"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUN9_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0695423.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUN9_002024"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUN9_002024"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0695424.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUN9_002025"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUN9_002025"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0695425.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUN9_002026"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUN9_002026"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0695426.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUN9_002027"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUN9_002027"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0695427.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUN9_002028"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUN9_002028"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0695428.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUN9_002029"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUN9_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0695429.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUN9_002030"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUN9_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0695430.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUN9_002031"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUN9_002031"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0695431.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUN9_002032"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUN9_002032"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0695432.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUN9_002033"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUN9_002033"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0695433.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUN9_002034"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUN9_002034"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0695434.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUN9_002035"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUN9_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0695435.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUN9_002036"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUN9_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUN9_002037"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUN9_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0695436.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4606 a         5269 c         4895 g         4760 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acaacaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZOYS010000066        19372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOYS010000066 DAZOYS010000000
VERSION     DAZOYS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.066"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUN9_002038"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUN9_002038"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0695437.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUN9_002039"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUN9_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0695438.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHUN9_002040"
     CDS             complement(3743..4162)
                     /locus_tag="ACHUN9_002040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0695439.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUN9_002041"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUN9_002041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0695440.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUN9_002042"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUN9_002042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0695441.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUN9_002043"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUN9_002043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0695442.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUN9_002044"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUN9_002044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0695443.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUN9_002045"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUN9_002045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0695444.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUN9_002046"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUN9_002046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0695445.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUN9_002047"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUN9_002047"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0695446.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUN9_002048"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUN9_002048"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0695447.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHUN9_002049"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHUN9_002049"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0695448.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHUN9_002050"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHUN9_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0695449.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHUN9_002051"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHUN9_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0695450.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHUN9_002052"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHUN9_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0695451.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="ACHUN9_002053"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="ACHUN9_002053"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0695452.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHUN9_002054"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHUN9_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0695453.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHUN9_002055"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHUN9_002055"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0695454.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4632 a         5124 c         4848 g         4768 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctagacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gg
//
LOCUS       DAZOYS010000067        19175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOYS010000067 DAZOYS010000000
VERSION     DAZOYS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.067"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(236..1885)
                     /gene="pgi"
                     /locus_tag="ACHUN9_002056"
     CDS             complement(236..1885)
                     /gene="pgi"
                     /locus_tag="ACHUN9_002056"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0695455.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2122..2318
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2410..3759
                     /gene="lysC"
                     /locus_tag="ACHUN9_002057"
     CDS             2410..3759
                     /gene="lysC"
                     /locus_tag="ACHUN9_002057"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0695456.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4011..4283
                     /locus_tag="ACHUN9_002058"
     CDS             4011..4283
                     /locus_tag="ACHUN9_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0695457.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4416..5288)
                     /gene="rluF"
                     /locus_tag="ACHUN9_002059"
     CDS             complement(4416..5288)
                     /gene="rluF"
                     /locus_tag="ACHUN9_002059"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0695458.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5636..6473
                     /locus_tag="ACHUN9_002060"
                     /pseudo
     CDS             5636..6473
                     /locus_tag="ACHUN9_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6543..7349
                     /locus_tag="ACHUN9_002061"
     CDS             6543..7349
                     /locus_tag="ACHUN9_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0695459.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7359..7766
                     /locus_tag="ACHUN9_002062"
     CDS             7359..7766
                     /locus_tag="ACHUN9_002062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0695460.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7766..8260
                     /locus_tag="ACHUN9_002063"
                     /pseudo
     CDS             7766..8260
                     /locus_tag="ACHUN9_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8326..9123
                     /locus_tag="ACHUN9_002064"
     CDS             8326..9123
                     /locus_tag="ACHUN9_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0695461.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9134..9958
                     /locus_tag="ACHUN9_002065"
     CDS             9134..9958
                     /locus_tag="ACHUN9_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0695462.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10010..11238
                     /gene="sorE"
                     /locus_tag="ACHUN9_002066"
                     /pseudo
     CDS             10010..11238
                     /gene="sorE"
                     /locus_tag="ACHUN9_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11621..12310
                     /gene="pepE"
                     /locus_tag="ACHUN9_002067"
     CDS             11621..12310
                     /gene="pepE"
                     /locus_tag="ACHUN9_002067"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR0695463.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12401..14032)
                     /locus_tag="ACHUN9_002068"
     CDS             complement(12401..14032)
                     /locus_tag="ACHUN9_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0695464.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14252..17935)
                     /gene="metH"
                     /locus_tag="ACHUN9_002069"
     CDS             complement(14252..17935)
                     /gene="metH"
                     /locus_tag="ACHUN9_002069"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0695465.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18135..18959
                     /gene="iclR"
                     /locus_tag="ACHUN9_002070"
     CDS             18135..18959
                     /gene="iclR"
                     /locus_tag="ACHUN9_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0695466.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4524 a         5015 c         5018 g         4618 t
ORIGIN      
        1 taaacaacga ttaaaaatct attttaggat taacctcacg gtatgatttc cgttaaatca
       61 cagacaaggg gatttatctg ataaaacaat gccgacttat tgtcggcatt gtttttaagg
      121 ttatttaact cctcatacta tgcattaatg tcgatattgg catcaggctc cggttgccgg
      181 atgcggcgta aacgccttat ccggcctaca tatcggcatc aggcacgcat gatgattaac
      241 cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg ctgctgattt
      301 ctttgtcatc tttcagctct ggcagaatac ggttcgccag ttgtttaccc aactccacgc
      361 cccactggtc gaaggtaaag atgttcagga tcacgccctg agtaaagatt ttgtgctcat
      421 acagcgcaat caacgcaccc aggctgaacg gagtgatttc gcgcagcagg atggagttgg
      481 tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt gccggatctt
      541 taccctgatc gcgatattcc tgctcaacca cttcgcggga tttaccaaac gccagcgctt
      601 cggtctgggc gaagaagtta gacagcagtt tctggtggtg atcagagagc gggttatggg
      661 tgatagccgg ggcgatgaaa tcgcacggta ccattttggt tccctggtgg atcagctggt
      721 agaacgcgtg ctgaccgtta gtgcctggtt caccccagat aatcgggcca gtctggtaat
      781 ccacaacgtt accgttacgg tcaacatact taccgttgga ctccatattg ccctgctgga
      841 agtacgccgc gaaacggtgc atatactggt catacggcag aatcgcttca gtttccgcac
      901 caaagaaatt gttgtaccag atgccaatca gcgccagcag gacaggcagg tttttctcgg
      961 caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc agaaagcagt tcaacgaagt
     1021 tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa gagtaacggc
     1081 cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac tcgccaacgg
     1141 ctttggcatt ggtggaaagc gccgcaaagt gttttgcaac gtgtttttca tcacctgccg
     1201 ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat agtttcctga gtggtgaagg
     1261 ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc acttcagcga
     1321 tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtgatttttg tacggacgca
     1381 gtgcttcggt caccatgtac gggccgaggt cagaaccacc gatcccgatg ttcactacgt
     1441 cagtgattgc tttgccggta taacccttcc actcaccgga gataatcgct tccgagaagg
     1501 ttttcatctt ctccagcacc gcgttgactt ccggcattac gtctttgcca tcaaccagaa
     1561 tcggggtatt gctacggtta cgcagcgcta cgtgcagcac ggcgcggttt tcagtgcggt
     1621 tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac tctttcgcca
     1681 gatcctgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa tccaccagca
     1741 tctgatcgtc gaatgttgcg gagaacttag aaaaacgatc gccgtcttta gcaaaaagat
     1801 cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc caggcagcgg
     1861 tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga attgtgactt
     1921 tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt tagcgtaatg
     1981 ttgagtgtaa acccttagcg cagtgaagca tttattagct gaactactga ccgccaggag
     2041 tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc ccgctcaccc tttatttata
     2101 aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta acggtgttgg
     2161 aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt gaacggctgg
     2221 ccacgttcat catcggctac aggggctgaa tcccctgggt tgtcaccaga agcgttcgca
     2281 gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga agtatcgctc
     2341 tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat cccctgacac
     2401 gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt agctgatttt
     2461 gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtgcg tttagtagtc
     2521 ctctcggctt ctgctggtat cactaatctg ctggtcgctt tagcggaagg gctggaacct
     2581 ggcgagcgat tcgaaaaact cgacgctatt cgcaacatcc agtttgccat tctggaacgt
     2641 ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tgctggagaa cattactgtt
     2701 ctggcagaag cggcggcgct ggcaacgtct ccggcgctga cagatgagct ggtcagccac
     2761 ggcgagctga tgtcgaccct gctgtttgtc gaaatcctgc gcgaacgcga tgttcaggca
     2821 cagtggtttg atgtacgtaa agtgatgcgt accaacgacc gatttggtcg tgcagagcca
     2881 gatgtagccg cgctggcgga actggccgcg ctgcagctgc tcccacgcct caatgaaggc
     2941 ttagtgatca cccagggatt tatcggtagc gaaaataaag gtcgtacaac gacgcttggc
     3001 cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc atctcgtgtt
     3061 gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtagt ttccgcagca
     3121 aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt tggtgcaaaa
     3181 gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccggt ctttgtcggc
     3241 tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga aaatccgccg
     3301 ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc tcactttgca cagcctgaat
     3361 atgctgcatt ctcgcggttt cctcgcggaa gttttcggca tcctcgcgcg gcataatatt
     3421 tcggtagact taatcaccac gtcagaagtg agcgtggcat taacccttga taccaccggt
     3481 tcaacctcca ctggcgatac gttgctgacg caatctctgc tgatggagct ttccgcactg
     3541 tgccgggtgg aagtagaaga aggtctggcg ctggtcgcgt tgattggcaa tgacctgtca
     3601 aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa cattcgcatg
     3661 atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga agatgccgag
     3721 caggtggtgc aaaaactgca tagtaatttg tttgagtaaa tactgtatgg cctggaagct
     3781 atatttcggg ccgtattgat tttcttgtca ctatgcttat caataaacga gcctgtactc
     3841 tgttaaccaa cgtctttatc gagaataatt gcctttaatt tttttatctg catctctaat
     3901 taattatcga aagagataaa tagttaagag aaggcaaaat gaatattatc agttctgctc
     3961 gcaaacgaat tcccgtacac tgaactctct ggctcactga gggcaccatc atggcactcc
     4021 cccgtattac ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa acactgctgg
     4081 atcgggcgcg gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga
     4141 aagagtacat cgataaatta atggtcgagc gtgaggctga agcgaaaaaa gcccgccaat
     4201 tgaagaaaaa gcaggcttat aaaccggatc ctgaagcgtc atttagctgg tcagcaaata
     4261 cgtcgacgcg cggcaggcgc taatgaacaa tcaacgcctg agattttcac tcgtcagtgt
     4321 gaacacaatt ttcccctcac cctaaccctt ttcccgaagg ggcgagggga ctgtccgagc
     4381 acttttgggc cgttttcatc agtctgaggc gttaatcagc gccccttctt tttacgcccc
     4441 ggcgaggtaa aacgcttacc gttggacgcc gggcgaccgc ctttttccgc cgttttttcc
     4501 atcttaacga ctggacgttt gatgcccgct gttttcggtt tggccttcgc tttgggcttc
     4561 gcctcggaag aggaattttc aatgagctta aagagatcga taagctcgtc gtcggttaaa
     4621 tcgcgccatt ctcccagcgg aatgccgctt aagctgacgt tcataatgcg cgtacgttcc
     4681 agctttttca cttcatagcc gaaatgctcg cacatgcgcc ggatctgacg gttcaacccc
     4741 tgcaccaggg taatgcggaa gacaaacggt gcttcttttt tgactttgca ctttttcgtc
     4801 accgtcccga ggatcggcac tcccgccccc ataccacgaa taaattcatc ggtaatcggt
     4861 ttatcgaccg tcaccagata ctctttctca tgatcgttac ccgcacggag gatcttattc
     4921 accagatcgc cgtgattggt gaggaaaatc agcccctggg agtctttatc caggcggcca
     4981 atcgggaaca cgcgtttgct gtggttaacg aaatcgacaa tgttatcgcg ctcgccatct
     5041 tcggtggtgc tgacaatacc gacgggcttg ttcagggcga taagtaccaa atcttcggct
     5101 tcccgaggct caatcaactg accgtttact ttcacaatgt cgccgggttt cacctgatca
     5161 ccaatggtgg ctcgcttgcc attaaggaac acattgcctt gctcgatata gcgatccgct
     5221 tcgcggcgtg agcaaattcc gctttcgctg atgtatttat ttaaacggac tgatgagtcg
     5281 ggcagcataa attctcctgt aaaacgcgga atataccgta cctcttagcg gggaaaaaca
     5341 gggttatgcg gcaacgtcga aattaaatgt gatctgccac gccttttatc acaaaagtgt
     5401 ctccggaagt acgatgcaaa tttgcctggc aaaataagga taatcaacat attacgaatc
     5461 gctgcacacc tgtgctgatg actactacgc agagaaacaa atgtgcaaga gaaagcgcta
     5521 tttctcccca tgcaaagagt gaaaaatcgc tttgaaaggc cctcataatg gggttatatc
     5581 agatcaagcg gtgagaaagc gtatttgcac aaatgtgcag aaccaggagg cggcaatgga
     5641 aaacagtgac gatatccgtt tgattgtgaa gattgcccaa ctctattacg aacaggatat
     5701 gacgcaggcg caaatcgcgc gcgaactggg tatttaccgc accaccatca gccgcttgct
     5761 taaacgaggc cgcgatcagg gaattgtcac catcgccatc aactatgact acaacgaaaa
     5821 tctctggctg gagcagcaac tgaagcaaaa gtttggcttg aaagacgttg tggtggtgtc
     5881 gggaaatgat gaggatgaag agactcaact ggcgatgatg gggttacacg gcgcgcaact
     5941 gctggatcgc ttgctggagc ctggcgatat tgtcggtttt tcctagggtc gcgcggtgag
     6001 cgcactggtt gaaaacttgc cgcaagcggg gcaatcgcgg cagttaatct gcgtgccaat
     6061 tattggcgga ccgtccggta aactcgaaag ccgctatcac gtaaacacat taacctacag
     6121 cgtggcagcg aagctgaaag gggaatcgca tctcgcggat tttccggctc tgctggataa
     6181 ccccttaatt cgtaatggga tcatgcagtc tcagcacttt aaaaccatct ctgcctactg
     6241 ggataatctg gatgtcgccc tggtgggaat tggctcaccg gccattcgcg acggcgctaa
     6301 ctggcatgcg ttttatggtg gtgaagagag tgacgacctg aaagcaggcg cggtattcca
     6361 ttggcattgc catgagtgaa gaaaaataca gcggaattgt tggtgcactg cgtggaaaat
     6421 atattaattg tctggtaacg aatagcagca cagctgaact attactgaaa taaaaatctt
     6481 cccgatgttt accgtaataa aaattacggc gggtccctat tatctaaaat caggagtctg
     6541 ttatgcaaac gtggttaaat ttgcaggata aaatcattat cgtcaccggc ggcgcatccg
     6601 gtattggtct ggcgattgta gaggaattat tagcacaagg cgcaaacgta cagatggtag
     6661 atattcacgg tggcgatggt caatatgaag ttcataaagg ctatcagttt tggccgaccg
     6721 atatttccag cgccaaagag gtaaatcata cggtagcgga aattatccag cgttttggtc
     6781 gcatcgacgg tctggtcaat aacgccgggg tcaatttccc gcgtctgctg gtcgatgaga
     6841 aagcgcctgc cgggcagtat gaactcaacg aagctgcatt cgaaaaaatg gtcaatatca
     6901 accaaaaagg cgtttttctg atgtcgcagg cggtggcgcg ccagatggtc aaacaacatg
     6961 atggcgtgat tgtgaatgtt tcctcagaaa gtggactgga aggctcagaa ggccaaagct
     7021 gttacgccgc taccaaagcc gcgctcaata gcttcacgcg ctcctggtcg aaagagctgg
     7081 gtaaacacgg tatccgtgtg gtcggtatcg cgccggggat tctggaaaaa accgggctac
     7141 gcacgccgga atatgaagag gcgctggcgt ggacgcgcaa tatcaccgtc gagcagctgc
     7201 gtgaaggcta taccaaaaac gccattccta ttgggcgcgc cggaagatta gcagaagtcg
     7261 ctgattttgt ttgttatctg ctgtctgaac gcgccagcta catcaccgga gtaaccacta
     7321 acattgcggg cggcaaaacg cgcggataag gaggctttat ggtcaacgcc attttttgtg
     7381 cccacggcaa attagcttgc gccatgctgg aatcggttca gatggtgtac ggcgacgcca
     7441 atgtcgaggc ggttgaattt gtaccaggcg agaacgccgg agacatcgta gcaaagctgg
     7501 aaaagttagt aagcattcac aatcaagatg agtggctgat tgccgtggat ttacagtgcg
     7561 gcagcccgtg gaatgcggca gcgatgcttg cgatgcgcaa tcctcgtctg cgggtaatca
     7621 gcggcctttc cctgccgctg gcgctcgaac tggttgataa ccaggacagc atgaatgttg
     7681 atgaactgtg tgaacacctg acgcaaatcg caaagcaaac ctgcgttgtc tggaaacaac
     7741 tggcaacggc agaggaagat ttctgatgaa cattacgctc gcccgcattg atgaccgtct
     7801 gatccacggc caggtcacta ccgtctggtc gaaagtggct aacgcccagc gcattattat
     7861 ctgcaatgac gaagtttata acgatgaagt gcgccggaca ttattgcgtc aggctgcgcc
     7921 accgggaatg aaggttaacg tggtcaatat tgaaaaagcc gtcgcggttt atcacaaccc
     7981 gcaatattag gacgaaaccg ttttttatct tttcactcgc ccacaggatg ccttagcgat
     8041 ggttcgccag ggggtaaaaa taggcaccct gaatattggc ggcatggcct ggcggcccgg
     8101 taaaaaacag ttaaccaaag cggtttcatt agatgatgat gatattaacg catttcatga
     8161 gttaaataat cttggcgtca taatggattt acgcgtcgta gcgtcagatc cctcaatcaa
     8221 tattatcgac aaaataaacg aacagttaat cgcaaattaa ataatagcgc ctgtgtacat
     8281 atgctttaac aggcagggat gacgacactt aaaaggtgac aaatcatgga aataagtacc
     8341 ctacaaatta ttgctatatt tcttttttcc tgtattgccg gaatgggcag cgtgctggat
     8401 gaatttcaga ctcatcgccc gttaattgcc tgtacggtga ttggtttaat tctcggtgat
     8461 ttaaaaaccg gaataatact cggtggtacg ctggaattga tagctctcgg ctggatgaac
     8521 gtcggcgcgg cgcaatctcc ggattctgca ctcgccagca taatctccgc cattctggtt
     8581 atcgttggtc agcaaagcat cgccaccgga atcgccatcg cgttgcctgt ggctgcggca
     8641 ggccaagtgc tgacggtgtt tgcccgtacc atcaccgtag tgttccagca cgcggcggat
     8701 aaagcagcgg aagaggcgcg gtttcgcact ctcgatattc tgcatgtctc cgcgcttggc
     8761 gtgcaggcgc tgcgcgttgc tattccggca ctgattgtct cactgttcgt cagcgccgat
     8821 atggtcagca atatgttaaa cgccattccc gaatttgtga cccgtggact gcaaattgct
     8881 ggcggtttta tcgtggtggt cggttacgcc atggtgctac gtatgatggg cgtgaaatat
     8941 ttgatgcctt tctttttcct cggtttcctc gcaggtggct acctcgatct cagcctgctg
     9001 gcgttcggtg gcgtcggcgt gatcatggcc ctggtctaca tccagctaaa tccacagtgg
     9061 cgtaaagctg aacctcaact ccaggccacc gcctccaccg cccttgacca acttgacgat
     9121 taacggagcc atcatggaac agagaaaaat tacacgcagc gatctggtga gcatgtttct
     9181 gcgctccaac ctgcaacagg cgtcctttaa ctttgaacgt attcacggac tgggcttttg
     9241 ctacgacatg atccccgcca tcaagcgact ttacccatta aaagaggatc aggttgcggc
     9301 gctcaggcga cacctggtgt tcttcaatac cacgccagcc gtatgtggcc cggtcatcgg
     9361 cgtcactgcc gccatggaag aggcgcgggc caacggcgcg gaaattgatg acggtaccat
     9421 taacggcatc aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg
     9481 gggaacgctg cgcccgatta ccgctgcgct cggcgcatct ctggcacttt ctggcaacat
     9541 cctcggcccg ctgctgtttt tctttatttt caatgcggtg cgtctggcga tgaagtggta
     9601 tggcctacag ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcgggaatgt
     9661 gctgcaaaaa ctcaccgaag gcgcgtcgat tctcggactg tttgtgatgg gcgtgctggt
     9721 caccaaatgg acgtcaatca acgtaccgtt ggtggtttca caaacgcatg ccgccgatgg
     9781 ctccaccgtc atcatgaccg tgcagaacat tctcgaccaa ctttgccctg gtttgctggc
     9841 gctcggtctg acgctactga tggttcgtct gctcaacaaa aaaattaacc cggtatggct
     9901 gattttcgcc ctgtttggct tagggattat cggcaatgcg ctgggcttcc tgtcctgatt
     9961 cttcgccccg gcacgactgc cggggccatc gctcaacatg aggtggttta tgaaaacaac
    10021 agctctgcgt ctttatggta aacgtgattt acgcctggaa acctttgacc ttcctgaaat
    10081 gcaggaggat gaaatcctcg cgacggtggt cactgacagc ctgtgcctct cttcctggaa
    10141 agaggcaacc tgggtgaaaa ccataaaaaa gtacccgacg atgtggcgac caacccaatc
    10201 atcatcggcc acgagttttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa
    10261 ttccagccgg gtcagcgtta tgtgattcag gccaacctga aactccctga ccgcccggac
    10321 tgccccggct actccttccc gtgggtgggc ggcgaagcca cgcatgtggt tattcccaac
    10381 gaggtcatgg aacaagattg cctgctggca tacgacggcg aaacctattt tgaaggctcg
    10441 ctggttgaac cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa
    10501 ggtagttata accacacgat ggggattcgc ccgcaagggc gcacgctgat cctcggcggc
    10561 accggaccaa tgggattgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg
    10621 ctgctcgtca ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca
    10681 tcagaaccgc aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta
    10741 atggcgctga gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga
    10801 ctggtgactc tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga
    10861 ccgcaggata aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc
    10921 cactacgtgg gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatt
    10981 gaagagaaaa aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg
    11041 ggcgaaacca cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg
    11101 aaatacctgc cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg
    11161 gcgcgtcatc aggggatctg gtccggtgag gcggagcaat atctgctcac tcatgcagag
    11221 gcaatttccc atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccgcgg
    11281 caacttcgcg acctgatgtg cggctgattg ctgaagcggc ccaatgtgca cgagaaggat
    11341 aaaaaaacat cagcgcctta aggggcgctc aaattggtgg caaagtacac attgtttatg
    11401 ccggatgcgg aataaagcct tattcggcct acataatctc gaaaattcaa tatattgcag
    11461 ttatctggta ggcctggtaa gcgtagcgca tcaggcaatg ctgtgtttgt catcagtctc
    11521 agcgcccggt gcaatacgtg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg
    11581 gtagcatgcc ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag
    11641 taactcgacg ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca
    11701 gttgcagggt cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga
    11761 tgattacaca gcgaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat
    11821 tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgct gagatcgtga ttgtcggggg
    11881 cgggaatact ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg caccaattac
    11941 tgacgtggtt aaacgtggtg cgctgtatat tggctggagc gcaggcgcta accttgcttg
    12001 cccaaccatt cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct
    12061 aaatctgttc ccgctgcaaa tcaacccgca cttcaccaac gcgctgccgg aaggccataa
    12121 aggtgaaacc cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat
    12181 tattggtcta ccggaaggta actggatcac ggtgagtaaa ggtcacgcta cgctgggtgg
    12241 cccgaacacc acttatgtgt ttaaggccgg tgaagaagcg gtgccgctgg aagcgggtca
    12301 ccgtttttaa gtgaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg
    12361 acgcttgacg cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt
    12421 catcttcatc cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcagac
    12481 gctgcatatc tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt
    12541 gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaagcgat
    12601 gcttgctgcg gcgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg
    12661 ccagctttaa attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa
    12721 gcgaaaatgc ccgccgtgct gccagcgatt tatcagcaat ttcgctgccc atgcgctcga
    12781 cgatatcgga agcctgttca aggttgagcg acatttcaat aatctccgcc cagcgacgcg
    12841 actcttcttc tgccagttcc tcttttggca ttcgcgccag atacagctta atggcggtgt
    12901 agagaacgtt gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt
    12961 gcatcacttt attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt
    13021 cgcgcgcggc gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt
    13081 taggccgtag ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg
    13141 ccatcgggtc aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga
    13201 aatagatcac cagttccgct tttggcaatg acaacttccc cattgtctct gccagcaaat
    13261 ggacaaacgg caggataatc aggctaccca ccagcttaaa cagcaaacta cccagcgcga
    13321 cacggcgggc tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga
    13381 ggttagcacc aatcaccaga cagagcgcca cggggaagga gataatgcct gcggcggtca
    13441 gagtcgcggt cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc
    13501 caatcagcgc atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt
    13561 ttgcctgggt gatcggcgtt acggcctgca caatcaactc cagcgccagc aaaatcagcc
    13621 caagaccaat accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa
    13681 aaatcacgcc gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac
    13741 gcgccattag cgccgtcccg acatctgcac ccagcacaat caccagagcc ggtgcgaggg
    13801 ctaccagatc ctgagcgacg aacgaggtca ccagcatggt ggtggcatta ctgctctgta
    13861 ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc
    13921 taaggacagt acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag
    13981 tcccccagac cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag
    14041 gttctcctta tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc
    14101 tgactggcat ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc
    14161 tgtttccagt ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt
    14221 ttgttgtaag gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg
    14281 ccagccagcg ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct
    14341 gatcgcgctg aatttgcgcc accgcgtagt acttgctgtc cgggtggctg aaataccaac
    14401 ccgaaaccga tgcaccaggc cacatggcga aagattctgt gagtttcatg ccagtatgtt
    14461 tttccacttc cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccgggt
    14521 agcccggtgc cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt
    14581 tctcgttcgg cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa
    14641 aggcttcggc taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct
    14701 gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc
    14761 cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag
    14821 cgaatcccgt tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc
    14881 gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaagccca
    14941 ccacgccacg cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt
    15001 ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact
    15061 tcccggccag cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagagttt
    15121 cgatgctggc ttcaacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct
    15181 gccagtcaaa agcgaagtcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgcg
    15241 gtttcttgcg cccgtgctga atacgtacgg tttcatactc cttgcgggta cgagcgacaa
    15301 aatcatcgcg ctgagtatcg gaaagcagcg ccgccaccac accaacggtg cgcgaggcat
    15361 tctgcacata caccgtcggg ccgctgtagt tctgctcaat tttcaccgcc gtgtgcgctt
    15421 ttgaggtcgt cgcgccaccg atcagtaacg gaatggtgaa gccctgacgc tccatctctt
    15481 tcgccacgtt aaccatctcg tccagcgacg gcgtgataag ccccgaaagt ccaatcagat
    15541 cagcattcac ttctttagcg gtacggagaa ttttttccgc aggcaccata acgccgagat
    15601 cgacaatttc gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt
    15661 ggacgtcgcc cttcacggtg gcgatcacca tcttgccgtt ggttttgccc tgctctttgc
    15721 tggcttcaat aaacggttcg aggtaggcca ctgcctgttt catgacgcgc gccgatttga
    15781 ccacctgtgg caggaacatt ttcccctcgc caaacagatc gccgaccacg ttcatgccgt
    15841 ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcgg
    15901 tatcctgctc gataaactca gtaatgcctt tgaccagcga gtattccaga cgtttattca
    15961 cttcccacga gcgccactcc gcctgctggg cgttagcggt gtcgtcggtt ttgctgccgc
    16021 gatatttctc ggcaagctcc agtaaacgtt cagtgccatc atcgcgacga ttaagaatca
    16081 catcttccac cgcgtcgcgc agttcagcgg gcaggtcgtc gtaaatcgcc agttgcccgg
    16141 cgttgacgat ccccatatcc atgccattgc gaatagcgta gtagaggaac accgcgtgaa
    16201 tggcttcgcg caccggttcg ttgccacgga atgagaaaga aacgttagat acgccgccgg
    16261 aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcgca ggcgccgata aagtcctgcg
    16321 catagttgtt gtgctcttca atgccggtgg cgaccgcgaa gatgttcggg tcgaagatga
    16381 tatcttctgg cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct
    16441 cgattttccg tgcgcgggta tcggcctgtc cctgttcgtc aaaggccatt accaccaccg
    16501 ctgcaccgta acgacgcaac agtttcgcat gatggataaa ggcgtcgacg ccctctttca
    16561 ttgagataga gttaacaatg cctttgccct gaatacactt cagccccttt tcgatgacct
    16621 cccattttga ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca
    16681 gattgagaaa acgcaccatc gccgcttcgg cgtcgagcat cccttcatcc atattgatat
    16741 cgataatctg cgcgccgttt tccacctgct ggcgggccac atccagtgcc tcgctgtatt
    16801 tctcttcttt gatcaggcgc ttgaacttag cggaaccggt gacgttggtg cgttcaccca
    16861 cgttcacaaa caggctatct tcgccaatgt tcagcggctc caggccggac aaacggcagg
    16921 ctacggggat ttccggcagt ttgcgcggcg ctaatccttc tactgcacga ctcatcgctg
    16981 caatatgttg tggcgtggtg ccacagcagc cgccgacgat attgagaaag cccgcttccg
    17041 cccattcacg tatttgtttt gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt
    17101 tgggtagccc ggcgttcggg tgcgcggtga cgtagcattc cgcaatccgt gacagctcct
    17161 gcacgtactg gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagcgctt
    17221 cggcgtggcg caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg
    17281 aggcgtcggt gatggtgcca gagatcataa tcggcagctc aacgcccagc gtttcaaact
    17341 ccgtttttac cgcaaatacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca
    17401 ggatcagatc cgcaccacct tccaccagcg ctttggtgga ctctcgataa gccgccacca
    17461 gcccgtcaaa agtgatatta cgaaatgccg gatcgttgac gtccggagaa atagacgccg
    17521 tgcggttggt tgggccgaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg
    17581 tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca
    17641 gggattccat ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga
    17701 tgatatccgc gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttac
    17761 tgagtaccag caggtcgttg ttgcctttga ggtcgcatgg ccagtcggca aagcgttcac
    17821 cacgaaaatc ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca
    17881 gcaccagaat acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg
    17941 ctcccgacac gctcaaccca gatttaataa aaattcaaca aaccatactg gcataaacgc
    18001 atctgtggta aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat
    18061 cagttcagca actattgcat taactaacaa taaaaatgaa aatgatttcc acgatacaga
    18121 aaaaggagac tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg
    18181 ccaccgcacc agcgactgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt
    18241 ggattgccga atccaatggc agtgtggcac tcacggaact ggcgcaacaa gccgggttac
    18301 ccaattccac gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg
    18361 ttggcgaact gggacactgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc
    18421 tccagagccg taatttgtta gcgattgttc accctatcct gcgcaatcta atggaagagt
    18481 ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg
    18541 atcaggtaca gtgtactcac ctgatgcgta tgtccgcacc tatcggcggt aaattgccga
    18601 tgcacgcttc cggtgcaggt aaagcctttt tagcccaact gagcgaagaa caggtgacga
    18661 agctgctgca ccgcaaaggg ttacatgcct atacccacgc aacgctggtg tctcctgtgc
    18721 atttaaaaga agatctcgcc caaacgcgca aacggggcta ttcatttgac gatgaggaac
    18781 atgcactggg gctacgttgc cttgcagcgt gtattttcga tgagcaccgt gaaccgtttg
    18841 ccgcaatttc tatttccgga ccgatttcac gtattaccga tgaccgcgtg accgagtttg
    18901 gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac
    18961 tttttctggc gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg
    19021 gtgtctgtta atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta
    19081 ttaactcagc attattttta aacatcaaac cacttaatta ttaacaaaat cgcaagatct
    19141 cattccaccg aaacttttac ggctgcatta tgaga
//
LOCUS       DAZOYS010000068        18881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOYS010000068 DAZOYS010000000
VERSION     DAZOYS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.068"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(83..2074)
                     /gene="tkt"
                     /locus_tag="ACHUN9_002071"
     CDS             complement(83..2074)
                     /gene="tkt"
                     /locus_tag="ACHUN9_002071"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0695467.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2352..3110
                     /gene="loiP"
                     /locus_tag="ACHUN9_002072"
                     /gene_synonym="yggG"
     CDS             2352..3110
                     /gene="loiP"
                     /locus_tag="ACHUN9_002072"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0695468.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3253..4173)
                     /gene="speB"
                     /locus_tag="ACHUN9_002073"
     CDS             complement(3253..4173)
                     /gene="speB"
                     /locus_tag="ACHUN9_002073"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0695469.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4311..6287)
                     /gene="speA"
                     /locus_tag="ACHUN9_002074"
     CDS             complement(4311..6287)
                     /gene="speA"
                     /locus_tag="ACHUN9_002074"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0695470.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6296..6427)
                     /gene="yqgB"
                     /locus_tag="ACHUN9_002075"
     CDS             complement(6296..6427)
                     /gene="yqgB"
                     /locus_tag="ACHUN9_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0695471.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6521..6664)
                     /gene="yqgG"
                     /locus_tag="ACHUN9_002076"
     CDS             complement(6521..6664)
                     /gene="yqgG"
                     /locus_tag="ACHUN9_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0695472.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6775..7026)
                     /gene="yqgD"
                     /locus_tag="ACHUN9_002077"
     CDS             complement(6775..7026)
                     /gene="yqgD"
                     /locus_tag="ACHUN9_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0695473.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7082..8236
                     /gene="metK"
                     /locus_tag="ACHUN9_002078"
     CDS             7082..8236
                     /gene="metK"
                     /locus_tag="ACHUN9_002078"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0695474.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8672..10066
                     /gene="galP"
                     /locus_tag="ACHUN9_002079"
     CDS             8672..10066
                     /gene="galP"
                     /locus_tag="ACHUN9_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0695475.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10143..10640
                     /locus_tag="ACHUN9_002080"
     CDS             10143..10640
                     /locus_tag="ACHUN9_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0695476.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10735..11442
                     /gene="endA"
                     /locus_tag="ACHUN9_002081"
     CDS             10735..11442
                     /gene="endA"
                     /locus_tag="ACHUN9_002081"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0695477.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11522..12253
                     /gene="rsmE"
                     /locus_tag="ACHUN9_002082"
     CDS             11522..12253
                     /gene="rsmE"
                     /locus_tag="ACHUN9_002082"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0695478.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12266..13216
                     /gene="gshB"
                     /locus_tag="ACHUN9_002083"
     CDS             12266..13216
                     /gene="gshB"
                     /locus_tag="ACHUN9_002083"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0695479.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13325..13888
                     /locus_tag="ACHUN9_002084"
     CDS             13325..13888
                     /locus_tag="ACHUN9_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0695480.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            13888..14304
                     /gene="ruvX"
                     /locus_tag="ACHUN9_002085"
     CDS             13888..14304
                     /gene="ruvX"
                     /locus_tag="ACHUN9_002085"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0695481.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14479..15459)
                     /locus_tag="ACHUN9_002086"
     CDS             complement(14479..15459)
                     /locus_tag="ACHUN9_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0695482.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            15477..16181
                     /gene="yggS"
                     /locus_tag="ACHUN9_002087"
     CDS             15477..16181
                     /gene="yggS"
                     /locus_tag="ACHUN9_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0695483.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16199..16765
                     /gene="yggT"
                     /locus_tag="ACHUN9_002088"
     CDS             16199..16765
                     /gene="yggT"
                     /locus_tag="ACHUN9_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0695484.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16762..17052
                     /gene="yggU"
                     /locus_tag="ACHUN9_002089"
     CDS             16762..17052
                     /gene="yggU"
                     /locus_tag="ACHUN9_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0695485.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17060..17653
                     /locus_tag="ACHUN9_002090"
     CDS             17060..17653
                     /locus_tag="ACHUN9_002090"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0695486.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17646..18782
                     /gene="hemW"
                     /locus_tag="ACHUN9_002091"
     CDS             17646..18782
                     /gene="hemW"
                     /locus_tag="ACHUN9_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0695487.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4500 a         5025 c         4927 g         4429 t
ORIGIN      
        1 tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt cgccgaagcg
       61 acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg acgttgtcga
      121 cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg aaggtggtca
      181 taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca atacccgctt
      241 ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa gccgcatcct
      301 gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg ccttcggcag
      361 tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg atgaagatca
      421 gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg tttgccagtt
      481 gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc gcggtcggac
      541 cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg tcacacggac
      601 gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc ggctggtgag
      661 tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc tgacgctgtt
      721 tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc aggaaggtgg
      781 aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg gtcataccga
      841 actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt gctttagaac
      901 cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg aattccggca
      961 acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg attttcgccg
     1021 gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca gacggcattt
     1081 cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc gcgtaagccg
     1141 caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca tcccactgcg
     1201 catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt tcgcgggtca
     1261 gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg gctttgttcg
     1321 gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca gtcactgcgc
     1381 gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg tcgcgaataa
     1441 cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag ccttcaacgt
     1501 gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg
     1561 tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg tcgcccatga
     1621 aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc gccgccagcg
     1681 ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg
     1741 tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta gagtgcagct
     1801 gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt
     1861 agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca gcccaggacg
     1921 gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg tcagccatac
     1981 ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac
     2041 gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg atgatgaagg
     2101 gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca
     2161 atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc agaaatctct
     2221 ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt ccttatactg
     2281 agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg accttgtgcg
     2341 gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca acggtactga
     2401 ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa gcttttcagg
     2461 cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt caggagatgg
     2521 acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg acaactattg
     2581 ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg tatatggcga
     2641 aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc gggctgatgg
     2701 atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg cacgtggcgt
     2761 taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg cgagtagctg
     2821 cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt gatctgggcg
     2881 agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat gattattctt
     2941 acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc tttgaaaaac
     3001 tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg gcatccgccg
     3061 aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa agcctggtgg
     3121 tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg cacatttgtt
     3181 accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta cgggtggcat
     3241 cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct ccagcgccag
     3301 cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca cttccactac
     3361 gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag cgcgatcgga
     3421 ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat ccaggcagtc
     3481 gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt gggcgataac
     3541 gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc cgttgtcttt
     3601 atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca gaccttcttt
     3661 cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt
     3721 gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag
     3781 cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg cagccagcag
     3841 cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa aggcatatac
     3901 cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg
     3961 gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac cgtggcgacc
     4021 acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc agtctgcatc
     4081 gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat tggaaaccag
     4141 tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac aaagtcgata
     4201 taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg tcacaataaa
     4261 ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac ttactcatct
     4321 tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg ttgttgcagt
     4381 tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag cgttttcgga
     4441 tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga cagttctact
     4501 tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc gaacaggttg
     4561 tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag catcggcgga
     4621 ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc atcaatatag
     4681 tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg acgttccggc
     4741 acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc ccatgcgtcc
     4801 ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg ttcctgcagt
     4861 tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg cacttcatgg
     4921 cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa
     4981 gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca ttcacgcaaa
     5041 gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct ttgcagcgcg
     5101 cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc cacgccgatg
     5161 atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc ggtgattacc
     5221 gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat gatgttgttg
     5281 gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt gccttcatag
     5341 tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag cttgtgcagt
     5401 tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat attcgccatc
     5461 tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc ttcacgcagg
     5521 gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt ttccccgccg
     5581 gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc cagacgagga
     5641 acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat cttctcaatg
     5701 accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat atattcgcgg
     5761 tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc cagtactgcc
     5821 atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg aatcagggac
     5881 tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta atcgccgtta
     5941 tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc
     6001 aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc acgagttttc
     6061 actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca cacgctgatg
     6121 tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt gtaagtacgc
     6181 agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg tagtacaccg
     6241 tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc gaacctcaaa
     6301 ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc gataacaacc
     6361 cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca accggtttct
     6421 ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg cgaaaccacg
     6481 agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg taaccagctc
     6541 ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa gcagagatgc
     6601 agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc agacaacggt
     6661 tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag ccaaaactct
     6721 gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc
     6781 cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa tacgcagaat
     6841 gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac acgattcaaa
     6901 aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt taatccatcc
     6961 ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg taggatccgc
     7021 taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg tgatattaaa
     7081 tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc
     7141 tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga aagcacgcgt
     7201 tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag
     7261 cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg gctatgtgca
     7321 ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg gcaaacagtc
     7381 tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca
     7441 gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag cacctatcac
     7501 ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg
     7561 gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca aaatcgttgg
     7621 tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga aatcgctgca
     7681 agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc tgacttctgc
     7741 caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga tgggtgactg
     7801 cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac gtcacggtgg
     7861 cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct acgcagcacg
     7921 ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc
     7981 ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg gtactgagaa
     8041 agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc gcccatacgg
     8101 tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag catacggtca
     8161 ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc
     8221 tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc gctggccttt
     8281 ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa agctgcccgc
     8341 acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact actgaaaccg
     8401 attacaccaa taacaccagc caaatatttg taatattttc atattattat tcagttttca
     8461 cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt taatctatga
     8521 tgatataact caattatttt catgcactta aaccataact aagataaatg ttagtgtaag
     8581 cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct atcttaattc
     8641 acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac aggggcggtc
     8701 aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat tactctttgg
     8761 cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat tccagattac
     8821 ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag tcggtgcggt
     8881 gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga tcggcgcaat
     8941 tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag tactgattct
     9001 ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac cgctgtacct
     9061 ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc agttgatgat
     9121 cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca ccggtgcatg
     9181 gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg gtgtcttctt
     9241 cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg ccgaacgcgt
     9301 gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg aaatccgtga
     9361 aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca acttccgccg
     9421 cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga tgaacgtcat
     9481 catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca cagagcaaat
     9541 gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg caatcggcct
     9601 tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga tggctgctgg
     9661 catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg cgcagtattt
     9721 cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg gtccgctgat
     9781 ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca tcacctgctc
     9841 cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga ccatgctcaa
     9901 cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac tgtttatcct
     9961 gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata ttgaacgtaa
    10021 cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc ctcaggcttc
    10081 ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta tcctctgccg
    10141 ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc ggcctgcggg
    10201 aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca aaactctctt
    10261 acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa atccgcctca
    10321 atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta ccgcacgaac
    10381 tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc aaagagtgga
    10441 agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag ttcgaactgc
    10501 aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat cagcttaccg
    10561 tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt cactgcggtg
    10621 aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc tggctgattg
    10681 cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta agtgatgtac
    10741 cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc ggcgttggcc
    10801 gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca cgctgacgcg
    10861 cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg cgttgttgat
    10921 ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg cgtagagtgg
    10981 gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca ggacggtgga
    11041 cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat gcataacctg
    11101 cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag ccagtggaat
    11161 ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga aaaagctgcc
    11221 gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat gcgcgaccaa
    11281 tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa caagatgtat
    11341 ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca aggcaatcat
    11401 aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact agcgggattc
    11461 tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg gatttttaac
    11521 tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc acatcgcgct
    11581 ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg ggcaggcgtt
    11641 gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg ccagcaaaaa
    11701 aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc cgctgcatat
    11761 tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc agaaatcgat
    11821 cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg ttaaactgga
    11881 tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg ctgcctgtga
    11941 gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg aagcctggtg
    12001 tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta acagcatcaa
    12061 tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag gcggtttatc
    12121 ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt tgggacctcg
    12181 cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag tacgatttgg
    12241 cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg accccatcgc
    12301 aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac agcgtcgtgg
    12361 ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg aagcccgcgc
    12421 ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt tcgtcggtga
    12481 acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc cgccgtttga
    12541 taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag ggacgctgat
    12601 cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg cctggttctc
    12661 tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag cgttctggga
    12721 gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt cgattttccg
    12781 cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg agcatggcac
    12841 tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca aacgcgtgct
    12901 ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg ggggcgaaac
    12961 ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg aaagtgactg
    13021 gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt ttgttggtct
    13081 ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct gtattcgtga
    13141 gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca tcgaagcacg
    13201 tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct gagtgacagc
    13261 gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa cagaacctct
    13321 gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg atccgatttt
    13381 ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg ggatcatcgt
    13441 caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga agatcacgcc
    13501 ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg gtccgctggc
    13561 tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca gcattcgcat
    13621 ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg gcaccgataa
    13681 acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag gtcaactgga
    13741 acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata ttctgttcaa
    13801 aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg atattctcac
    13861 catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt cgacttcggc
    13921 accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg ccctttgcct
    13981 gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt actgaaagag
    14041 tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac cgagcaacca
    14101 ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg tgttgaagta
    14161 aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt tgaacagggc
    14221 ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat tattctcgaa
    14281 agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt cgttaccgcc
    14341 atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga ttgtaggtct
    14401 gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg ataagcttgc
    14461 acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa aactctgctg
    14521 aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact ggtgggtttt
    14581 cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa acagcgccac
    14641 gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta aactacctgc
    14701 cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca gtcgctcaac
    14761 tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg tttctgccgc
    14821 cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa tcacatcagg
    14881 atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca aaccaatttc
    14941 ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat cttccagcgt
    15001 cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca gcgtggtaga
    15061 tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct tgagtaattc
    15121 cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag gtaacagccg
    15181 taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc gctggttttc
    15241 cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc gctgatcgtc
    15301 atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg cagcttccat
    15361 tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt
    15421 atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct cggaaaatga
    15481 acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt
    15541 gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg
    15601 ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac gttcaggaag
    15661 gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa tggcatttta
    15721 ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac tggtgtcata
    15781 ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca gaacttcccc
    15841 ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc gggattcaac
    15901 tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg
    15961 ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc
    16021 aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac acgctctctc
    16081 tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg
    16141 gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa ggaacgccat
    16201 gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg tgctgttatt
    16261 acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac agtttgtagt
    16321 gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa tgggccccat
    16381 tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca tcgtgctgtt
    16441 taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc tgctgaaaac
    16501 catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct gggtaagcca
    16561 gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc tgcgcccgat
    16621 tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc tcgttctgct
    16681 gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa acatgctgct
    16741 gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg tctggtttta
    16801 cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca tggcgacgaa
    16861 gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca tctggtgaag
    16921 tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt
    16981 ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg
    17041 ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt
    17101 gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc aaacagacct
    17161 cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga ttctgaaagc
    17221 gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg gtctggcggt
    17281 agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg aagacgcgac
    17341 cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg acgaccaacg
    17401 tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc cgactccgct
    17461 ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt
    17521 tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg aactgacccg
    17581 cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt
    17641 acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg tgcgtgcaga
    17701 aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg cacgacgatt
    17761 atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag ggccgtgaag
    17821 taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa
    17881 cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa attactatgg
    17941 aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt gctggtgtga
    18001 accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga cttgggcgta
    18061 ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg gggctgcgta
    18121 gctttaacct cgatttgatg catggactac cggatcaatc actggaagag gcgcttggcg
    18181 atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg
    18241 aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac gcgctgtggg
    18301 atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa tatgaaactt
    18361 ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg cgatttggtg
    18421 actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat gggcgcattc
    18481 tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat ctggaaagcc
    18541 agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc ttccgtctgc
    18601 tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa gatgtgattc
    18661 gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg gattactggc
    18721 agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc ctcgctgagt
    18781 aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta attaacttcg
    18841 gcaaaatata tttcttactg aataaacatc gatgatattt a
//
LOCUS       DAZOYS010000069        18842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOYS010000069 DAZOYS010000000
VERSION     DAZOYS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.069"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUN9_002092"
     CDS             complement(<1..101)
                     /locus_tag="ACHUN9_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695488.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUN9_002093"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUN9_002093"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0695489.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUN9_002094"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUN9_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0695490.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUN9_002095"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUN9_002095"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0695491.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUN9_002096"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUN9_002096"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0695492.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUN9_002097"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUN9_002097"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0695493.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUN9_002098"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUN9_002098"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0695494.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUN9_002099"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUN9_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0695495.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHUN9_002100"
     CDS             9871..10449
                     /locus_tag="ACHUN9_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0695496.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUN9_002101"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUN9_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0695497.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUN9_002102"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUN9_002102"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0695498.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUN9_002103"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUN9_002103"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0695499.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="ACHUN9_002104"
     CDS             15379..15993
                     /locus_tag="ACHUN9_002104"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0695500.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="ACHUN9_002105"
     CDS             15993..16322
                     /locus_tag="ACHUN9_002105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0695501.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUN9_002106"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUN9_002106"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0695502.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="ACHUN9_002107"
     CDS             17409..17720
                     /locus_tag="ACHUN9_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0695503.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="ACHUN9_002108"
     CDS             17704..18246
                     /locus_tag="ACHUN9_002108"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0695504.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18842)
                     /locus_tag="ACHUN9_002109"
     CDS             complement(18302..>18842)
                     /locus_tag="ACHUN9_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0695505.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4956 c         4581 g         5091 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcca gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg cttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaactctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cc
//
LOCUS       DAZOYS010000070        18664 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOYS010000070 DAZOYS010000000
VERSION     DAZOYS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.070"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..364
                     /gene="ydjO"
                     /locus_tag="ACHUN9_002110"
                     /pseudo
     CDS             1..364
                     /gene="ydjO"
                     /locus_tag="ACHUN9_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHUN9_002111"
     CDS             complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHUN9_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0695506.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1892..2482)
                     /locus_tag="ACHUN9_002112"
     CDS             complement(1892..2482)
                     /locus_tag="ACHUN9_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0695507.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHUN9_002113"
     CDS             complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHUN9_002113"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0695508.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3460..3996
                     /locus_tag="ACHUN9_002114"
     CDS             3460..3996
                     /locus_tag="ACHUN9_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0695509.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4037..4897)
                     /locus_tag="ACHUN9_002115"
     CDS             complement(4037..4897)
                     /locus_tag="ACHUN9_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0695510.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHUN9_002116"
     CDS             complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHUN9_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0695511.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHUN9_002117"
     CDS             complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHUN9_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0695512.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6610..7368
                     /locus_tag="ACHUN9_002118"
     CDS             6610..7368
                     /locus_tag="ACHUN9_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0695513.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7421..7528)
                     /gene="yniD"
                     /locus_tag="ACHUN9_002119"
     CDS             complement(7421..7528)
                     /gene="yniD"
                     /locus_tag="ACHUN9_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0695514.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7700..9598)
                     /gene="espL1"
                     /locus_tag="ACHUN9_002120"
                     /pseudo
     CDS             complement(7700..9598)
                     /gene="espL1"
                     /locus_tag="ACHUN9_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10123..12051
                     /gene="thrS"
                     /locus_tag="ACHUN9_002121"
     CDS             10123..12051
                     /gene="thrS"
                     /locus_tag="ACHUN9_002121"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0695515.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12055..12597
                     /gene="infC"
                     /locus_tag="ACHUN9_002122"
     CDS             12055..12597
                     /gene="infC"
                     /locus_tag="ACHUN9_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0695516.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12694..12891
                     /gene="rpmI"
                     /locus_tag="ACHUN9_002123"
     CDS             12694..12891
                     /gene="rpmI"
                     /locus_tag="ACHUN9_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0695517.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12944..13300
                     /gene="rplT"
                     /locus_tag="ACHUN9_002124"
     CDS             12944..13300
                     /gene="rplT"
                     /locus_tag="ACHUN9_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0695518.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13417..13541
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13423..13467
                     /gene="pheM"
                     /locus_tag="ACHUN9_002125"
     CDS             13423..13467
                     /gene="pheM"
                     /locus_tag="ACHUN9_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0695519.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13750..14733
                     /gene="pheS"
                     /locus_tag="ACHUN9_002126"
     CDS             13750..14733
                     /gene="pheS"
                     /locus_tag="ACHUN9_002126"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0695520.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14748..17135
                     /gene="pheT"
                     /locus_tag="ACHUN9_002127"
     CDS             14748..17135
                     /gene="pheT"
                     /locus_tag="ACHUN9_002127"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0695521.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17140..17439
                     /gene="ihfA"
                     /locus_tag="ACHUN9_002128"
     CDS             17140..17439
                     /gene="ihfA"
                     /locus_tag="ACHUN9_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0695522.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(17746..17886)
                     /locus_tag="ACHUN9_002129"
     CDS             complement(17746..17886)
                     /locus_tag="ACHUN9_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0695523.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(18077..18337)
                     /locus_tag="ACHUN9_002130"
     CDS             complement(18077..18337)
                     /locus_tag="ACHUN9_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR0695524.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
BASE COUNT         4866 a         4349 c         4658 g         4791 t
ORIGIN      
        1 atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt
       61 tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg
      121 aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg
      181 tctcgggtat gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat
      241 tgataagttg gacatttttt tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc
      301 cgcgatggaa catttaaaaa gtaaagcaac ggcccctata caaaacggac cgttgccagc
      361 ataagaatta acggtgtgcc agttcggcgt cgtcttcgct atccagaatg gctttatcgg
      421 tttgcttcag ccactggctg gtcagcgtgc cagctgtcat cgagccacta acgtttagcg
      481 ccgtacggcc catgtcgata agcggttcaa cggagattaa cagcgccacc agggttactg
      541 gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg
      601 caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac atcgggtcca
      661 gcgggttaat gccaaccgta ggcgcaacca tcaccgccag cattgccgga tacaaaccgg
      721 cgcagccgtt ctgaccaatg gttgcaccga aagaggcggc gaaactggcg atggattcag
      781 gaacgcccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca gcactggaac
      841 ggctggtaaa ggcaaacgtc agcacaggcc ataccttacg gaagtacttc agcggactca
      901 cgccattaat gcccagcaga atgccatgca ctgcaaacat aatcagcaga ccgaggtagg
      961 acgcgacaac gaaacttccc agtttgatga tgtcttgcaa gttagaacct gcaaccactt
     1021 tggtcattag agccagaacg ccgtaagggg tcaactgcat gacaaggcga accagtttca
     1081 tcacccagct ttgtagggta tcgatagcgg ctaagacgcg ttcacctttc ggcgcatcat
     1141 ccttcagcag tttcaatgca gctacgccga ggaatgcggc aaaaattacc acgctgataa
     1201 ttgacgtcgg attggctccg gtaagatcgg caaacgggtt tttcgggata aaggacaaga
     1261 ccagctgcgg aacgctcaga tcagagactt taccaacata gttactttca atggcgttca
     1321 gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca aacaggttgg
     1381 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     1441 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     1501 aggcgaagac taacggcata acgatcattt gcagcagttg aacatagccg ttaccaacga
     1561 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     1621 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     1681 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     1741 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     1801 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     1861 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     1921 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     1981 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     2041 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     2101 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     2161 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     2221 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     2281 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     2341 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     2401 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     2461 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     2521 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     2581 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     2641 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     2701 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     2761 gggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     2821 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     2881 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     2941 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     3001 tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     3061 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     3121 gccgggcgta ccaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct
     3181 cgttacgacg ggagatatcc acccccaggc ttgccatcac atccagttcg gctcgatccc
     3241 ataaaggttc tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac
     3301 gcggggttga cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct
     3361 tatgattaag cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt
     3421 aaactaagtg aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga
     3481 cgcattgctg ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctcc
     3541 acattgattt ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat
     3601 gcggtcatgc tcgactttac tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt
     3661 ttgaatctgt tctggtataa ctccccgacc cctaatttta atggtggcgt gaacgttatg
     3721 ttctgggtga ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg
     3781 atgagtcgcc aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa
     3841 gccaaagggg aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac
     3901 catactattt tcctgcagtt tttctcacta tatatcctgc cggttatttg tattgctgca
     3961 ggttatgtgt tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca
     4021 ttatcctcaa cccatatcat gctgctaata atctatccaa tgactgctga gcaataacca
     4081 aatgctgacc gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt
     4141 gacgttcaag gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg
     4201 gcggctgttc agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc
     4261 aggcagggtc gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat
     4321 cgccgtgcaa cagagaaggc tgcagttgat gcgaggccag acgctgctga atatgctcga
     4381 cgatggcgtc gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc
     4441 caatccgttg ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag
     4501 ttgtggagag cgagttatca aaatcgaggc caaattgtgg ttggtcactc cattgatgta
     4561 aacgcgcaat ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga
     4621 gataatccat caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa accttaggca
     4681 cggtgacggt tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaaccgg
     4741 gaagcagttc cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc
     4801 atgcggcatg gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta
     4861 actgctcgct caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc
     4921 cagatcataa ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga
     4981 ctgattattg cccaaaaacc atttattttc tgttgctatt ccattcctcc caagtcgtga
     5041 tggtgatttc aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa
     5101 tttcttcttc actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat
     5161 ttccctcatc atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact
     5221 cgttaatttc agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat
     5281 cgccactggc catagttcac ccctttgtta tcacatgact tttgagcaaa gtcggagaaa
     5341 cgcgttgccg acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg
     5401 gaaaggtaag cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg
     5461 agtgtggctg cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt
     5521 tctgccagtt tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg
     5581 cttttcaccg gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct
     5641 tgtggaccaa gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg
     5701 gctttgcgga catcgtccgg ctgggtgagt tcgcgattca ccagcgcact gagttctttt
     5761 tggttaggct taaccaactc gatgttacca attgccagtg ctgcacttaa cgcttcgcca
     5821 gaactgtcga cgatgcagcg gatcccttgt ttttgcgaag cggaaatcag ttgggttaat
     5881 ttttccagct tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca
     5941 atttccagaa cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc
     6001 ataacaaaac gatactgctc accgcttgct tccacatgta catgtaaatt ctgccgggtc
     6061 cagtctttgg cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg
     6121 tgttcgccgg tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca
     6181 atggcgcggg cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc
     6241 agttttcctt cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt
     6301 gtcaacgtat agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga
     6361 atcagcggaa agtgaagaaa ttaacaatat gatttattgg agctttattt aaaattttgc
     6421 agataaatat atataaataa aaatctgttt tcgccacatg cttattcaaa aaatgaaata
     6481 agaatacatt gctaagttat tcgcgccttt ttataattcg ttacctttcc agggacgcta
     6541 atctttgatc cttaagaaag tttccgggac cgcgatggtc tctttttttg ttgtacggac
     6601 tcttgataaa tgaagctttt gaagacagtt cctgccatcg ttatgttggc ggggggcatg
     6661 tttgcgtcac tgaatgcagc tgccgatgat tccgttttta ctgtcatgga cgatcctgcc
     6721 agcgctaaaa aacctttcga aggtaatctg aacgcaggat atcttgcaca atcaggcaat
     6781 acgaaaagct cctcacttac ggctgacacc accatgacct ggtatggaca aaccactgcc
     6841 tggtcgctgt gggggaacgc cagtaatact tcttctaatg atgagcgttc ttcagaaaaa
     6901 tatgcggcgg gcgggcgtag ccgttttaat ttaactgatt atgactattt atttggtcag
     6961 gcaagctggc tgacagaccg ttataacggc tatcgagagc gcgatgtgtt gaccgcgggt
     7021 tatggtcgcc agtttttgaa tggcccggta cacagtttcc gctttgaatt cggcccaggt
     7081 gtgcgttacg acaaatatac cgacaatgcc agtgaaaccc agccgctggg ctatgcctcc
     7141 ggggcttatg catggcagtt gactgacaac gctaaattta cgcagggtgt ttcggtcttt
     7201 ggcgcggaag atacaacgct gaactccgaa agcgcattaa atgttgctat taatgaacat
     7261 tttggactga aggtggctta caacgtcaca tggaactctg aacctccaga gtctgcaccg
     7321 gaacataccg atcgtcggac aaccttatcg ctgggctaca gcatgtaaaa ttatctgccg
     7381 gaccgtgtta tgcggtctgg ctgtcttcgt ttgtcactgc tcagccccaa atcattgctg
     7441 cccaacgtag caacaacata gcgccacaga ttgccagtag caccaccacc atcttccagt
     7501 gttttttatc aaagcgttta gtcggcatac cagtgtcctg catatctctt gtgcgtgata
     7561 acggagtgta acaaaaggga taggcatcaa gggatttttt gttattacag ccgtcgggtg
     7621 gcgttgtgca catggtcact gtttctgcgt tttatgaaaa aaataaaatt atgtatcgat
     7681 atatatcgta aatagctggt tataaatacg tctttatttc atcagcacta aaactcaggc
     7741 tttggctgat gacatttata gcagcataat atgtttctac agttttataa tgcttatgat
     7801 gaatggctat atgaacagct gacatgttgt catgattttt agcggccaat agtgtaaata
     7861 actgatgttg actaaaagaa tgtttttttg caaaagcacc cagcgtcgat atataagata
     7921 acacgacgtc ttcattaccc ttgctcatgg cgatgtataa tgcaggggtt ccctgtgctg
     7981 tagctccttt taataaatcc atgatgttag ctttgctcaa cttgtattta aaggcgatgc
     8041 cattaatttt agagaggaac cgtgtgacac acaaagggtg attattttcc attgcggcgt
     8101 ataatcctgg ctcaccatca tgtattttca tggctaaaat actcatcacc atttttttgt
     8161 ttaaaagttc ttgtgctatt ggagtggtta atgcattcag aaagatatcg attatttcat
     8221 cgaatccata caacatggag atatagagtc caggtgtttc gttagcactt ttagtttgca
     8281 gtaatttaac cagattatct tcatgaatga tatgattgta taccagtgac tgaatttctt
     8341 gcataaatat ttttacgttg tcagtttgtc cgttcatcat agccagaaat aaagcaggtg
     8401 taccaacgct attctgcgca gttattattt caatgatttc tttttcaggg agtatgggca
     8461 actgtttgaa tagtgtaata agctattgag tctggcctgt ggccattatt aacaacattg
     8521 ccgatggatg tataggggca aatggcattc gtgtcgtgat acccgctaaa atagcaactg
     8581 gaactccctc aggtaataga ggaagaatag caattgcaca gttgcgaaca tacctatcag
     8641 cccaggtctg agcatcggga gctgaactga tgaacttatc aagcgagaga aagccccgtg
     8701 ctgccctgtg ggtgcctctg acggcaacgt tggtatcgtt cgggtcgtaa acagacacaa
     8761 cataatttaa ttgtccatac actgactgct ttattcgtag ccttactgac aaagtatgat
     8821 tgtctatata taaaatcgcg gacatatgac tgacattgtt tgccatcatt cgacaaaaga
     8881 aaatatctaa agcgaagcca aaatccttac tcgcgataac gcgactccct gcaggaggat
     8941 ggcgaagtaa tgctctgaac tccgcgtccc ggactaaggg gacatgctgc tgtatggatt
     9001 caacggagga gtagtgttta atcaggttga ctttgccttt tccatttctc aatgaatcaa
     9061 gggcgtattg caatgacaga tggcgacaaa aaatagcgtc agaaggagat tgcgaaaaaa
     9121 atgcactacc attgagattt actgttggtt gtatagtttt ttctctatgg atttttttag
     9181 acagtttcat acaatggtta cgatcttcgg cagttaactg ttcctgttca atataatcca
     9241 gtatgcttcg caaatggtta gccttaatag gcgtggtttc cagaaataag ggaatgattt
     9301 cttttttata gtgtaatgta atgagtatat tttctgcatg gatgggtgaa gtggcaaagt
     9361 taatgataaa aagggcagtg gaataacaat tttgtgaaat ttttcgacag tttgcggtaa
     9421 attcctgagt aacacagtat gacagcatcg aacttaagtc agaggtatca agtagggtga
     9481 tgagttggcg cgcaaattga ttaataggct ctgatgaagg cgttggtatg tcgctatcga
     9541 tgtgcaatct atgaggagac ttataatggg ttctgataat gatatcgttt tgactcataa
     9601 tctattcgcc ttatatatgt ctggaggttt tgaggatgat gattaattat tatcgccaga
     9661 acgaaatgtg cacgaaaaat tgctataaga attgtgtcac ttttatcatt gtgatatgac
     9721 cacttcgtca ttaatctcat gagcgtttaa aatactaccg ccaaatcacg caacgaactt
     9781 tgctggattc agtttatgct gctgtaaatc cgctcgcagt aaacctttca gacgcaccgt
     9841 gatgttatca gtggttcttt aagcgttttg ctggtgtact cactacaaac gaattgcgaa
     9901 tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa catcgttcaa
     9961 ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg aaccaattta
    10021 gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt tctttgtatg
    10081 tgatctttcg tgtgggtcac cactgcaaat taaggatata aaatgcctgt tataactctt
    10141 cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt tgcgctggac
    10201 attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga actggttgat
    10261 gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa
    10321 ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcatgcgat taaacaactt
    10381 tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta ttacgacgtt
    10441 gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag
    10501 cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcacga agcgcgtgaa
    10561 actttcgcca accgtgggga gagctacaaa gtctccattc ttgacgaaaa catcgcccat
    10621 gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg cggtccgcac
    10681 gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg ggcttactgg
    10741 cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa
    10801 aaagcactta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa
    10861 atcggtaaac agctcgacct gtaccatatg caggaagaag cgccgggtat ggtattctgg
    10921 cacaacgacg gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa
    10981 gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa
    11041 accggtcact gggacaacta caaagatgca atgttcacca catcttctga gaaccgtgaa
    11101 tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag
    11161 tcttatcgcg atctgccgct gcgtatggcc gagtttggta gctgccaccg taacgagccg
    11221 tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta cccaggatga cgcgcatatc
    11281 ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat
    11341 atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa
    11401 cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa
    11461 gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt
    11521 gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac
    11581 ttctccttgc cgtctcgttt gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta
    11641 ccggtaatga ttcaccgcgc aattctgggg tcgatggaac gtttcatcgg tatcctgacc
    11701 gaagaattcg ctggtttctt cccgacctgg cttgcgccag ttcaggttgt tatcatgaat
    11761 attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc aaatgcgggc
    11821 attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact
    11881 ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag aggtggaatc aggcaaagtt
    11941 gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag
    12001 aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa
    12061 ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag
    12121 gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct
    12181 ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg
    12241 ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag
    12301 gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctggtaca
    12361 gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat
    12421 aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg
    12481 gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtggtcga atccttccca
    12541 acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc
    12601 cttcaagtag cactgtctgt ggagccttcg ggcttcacag gctttgttcg cctttgtttc
    12661 gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt
    12721 gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg
    12781 cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg
    12841 gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata agccgttaac
    12901 gtttttaact ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg
    12961 tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg
    13021 tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc
    13081 ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa
    13141 cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc
    13201 ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt
    13261 caccgctctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agagggagct
    13321 agtctccctc ttttcgtttc aacgccatca aaacattgac ttttatcgcc gtagcctttt
    13381 caataaaggt cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc tgctattttc
    13441 cgcttctttt tttactttag cacctgaatc caggaggcta gcgcgtgaga agagaaacgg
    13501 aaaacagcgc ctgaaagcct cccagtggag gctttttttg tatgcgcgtt ttgaaaaata
    13561 aaaagccagt ctatcaggcg taattgtcgc agccagtctg gacacggcca gcgcggagaa
    13621 accggagcgt atttaagtac gtgagaattt cgagcactgc ccgggaccaa aatggcaagt
    13681 aaaatagcct gatgggatag gctctaagtc caacgaacca gtgtcaccac tgacacaatg
    13741 aggaaaacca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc cattagccag
    13801 gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa aaaagggcac
    13861 ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt
    13921 gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa
    13981 ctggaaagcg cagcgctgaa tgcgcgtctg gcggcggaaa cgattgatgt ctccctgcta
    14041 ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa
    14101 agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga agacgattat
    14161 cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga ccacgacact
    14221 ttctggtttg acactacccg cctgctgcgt acccagacct ctggcgtaca gatccgcacc
    14281 atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgcgttta tcgtaacgac
    14341 tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt tgataccaac
    14401 atcagcttta ccaacctgaa aggcactctg cacgatttcc tgcgtaactt ctttgaggaa
    14461 gatttgcaga ttcgcttccg tccttcctac ttcccgttca ccgagccttc tgcagaagtt
    14521 gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg gttgcgggat ggtgcatccg
    14581 aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc cttcgggatg
    14641 gggatggagc gtctgactat gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa
    14701 aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg aaattcagtg
    14761 aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgatgcgctg gcgaatcaaa
    14821 tcactatggc gggcctggaa gttgacggtg tagaaccggt tgccggtagc ttccacggcg
    14881 tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga
    14941 caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgtggtgcg ccaaactgcc
    15001 gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt gatttcaaaa
    15061 ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc ttctctgaac
    15121 tgggaatttc tgacgatcac agcggcatta tcgaactgcc tgcagatgcg ccgattggca
    15181 ctgacatccg cgaatacctg aaactcgatg acaacaccat cgaaatcagc gtaacgccaa
    15241 accgtgccga ctgcttaggt atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc
    15301 tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac acgttgccga
    15361 ttacagtcga agcgcctgaa gcctgcccgc gctatcttgg ccgtgtggta aaaggcatta
    15421 acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt
    15481 ctatcgatgc agttgttgac gtcaccaact atgtactgct cgaactgggc cagccgatgc
    15541 acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca aaagagggcg
    15601 aaacgctggt gctgcttgat ggcactgaag cgaagctgaa tgctgacact ctggtcatcg
    15661 ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac tctggcgtga
    15721 atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg tctatcaccg
    15781 gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca ccgttatgag cgtggcgttg
    15841 atccggcact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc gacatctgcg
    15901 gtggtgaggc tggtccggta attgatatca ccaacgaagc aacgctgccg aagcgtgcaa
    15961 ccatcacttt acgtcgtagc aaactggatc gcctgatcgg ccatcatatt gctgatgaac
    16021 aggtaactga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc
    16081 aggcagttgc gccgagctgg cgtttcgata tggagattga agaagatctg gttgaagaag
    16141 tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca agcctgatta
    16201 tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca
    16261 aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag cagatgatcc
    16321 atccaggcgt tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc
    16381 gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga
    16441 accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc agatactcag gcaccgttgg
    16501 gcattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac gaagagcact
    16561 ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgatctt gaatccgttc
    16621 tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat ccggcactgc
    16681 atccggggca atccgcagcg atttatctga aaggggaacg tattggtttt gttggagttg
    16741 ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg ttcgaactgg
    16801 agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg
    16861 cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg gatattttat
    16921 ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt gacgtgtacc
    16981 gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc ctgcaagata
    17041 ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt gtagaggcat
    17101 taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac aaaagctgaa
    17161 atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa agaactggtt
    17221 gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct
    17281 ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg
    17341 ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg gcagaagtta
    17401 aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc
    17461 gctctgcggc cttttttctt tcaataactg attgatttat aatatcttat gccgcttcgt
    17521 tccactgtgt gtccacattc agtggagggt gaggggagta agcccgctac attgcgggct
    17581 ttgtcttata gtcttatcac tgtttcaagt cggtgagggc accaaaaaga aaacccgcag
    17641 tttttacgct gcgggtttgt tgttcatatc tgtgagatag ggtgccttat ccggccttac
    17701 cctggcaacc gattgacggg ggattgctcc cccgtcgcgg tttccttact gcttacactg
    17761 taagaacgcc gcaaactccg ctccccagaa gctcatccgt atttcacaca gcgaaccgtg
    17821 caacatccag atgatgagga ttgccgtcac gcagaacgtg atggtcgtaa gcgatttttg
    17881 cgacatagca cttgctcctt ttccggagag gcgctaacct ttcacttgtc aaggtaatgc
    17941 ggttagggcc tcggttaaac agagatgttt tccggggcct ttccacatct ggccttcggg
    18001 tattccctcc gaccatcagc cgaaaggcac ccgcgcgtaa tctatcgctt ttttgttact
    18061 ccggcaattc tgcctgttaa ttctgaggta aaggcaagct catctgattg tttcccctgt
    18121 gtgaagctgg cagatcatgc cacgggatat cttctgaaga gtgaacgccg gaggcgtgtt
    18181 ttgatgtgaa tttatggaaa gcttccagtg ttgagaagca tacgccgcat tccagattgt
    18241 tacactggta atacttttgc cgcacggtgt ttgaatcatt ttccggacga ctggtgcgga
    18301 tacgggcaga tgcgccacaa agcggacaac ggaacatagc gacctccctt aacgtagtgc
    18361 aggggctatt ctaagttgtt ctggctcata ctccaacagc cagtccgttt cgagcgaaaa
    18421 gcggtgattt aactcttgaa gaaataggct ttgatacgat ttatccaaag cctgtaaaag
    18481 attggaatgt tttttatcct gtcatctatt ttgggaaaag atcaggaaat agttatgcac
    18541 ttatagattt actatagcat tgtattattt gagtacaccc tcatgttttg atttgtgcgc
    18601 taatagaact tttctgtctt ttcttctgct cttgaatgta tgtctgcacc atttctgtta
    18661 tggt
//
LOCUS       DAZOYS010000071        18515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOYS010000071 DAZOYS010000000
VERSION     DAZOYS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.071"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHUN9_002131"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHUN9_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0695525.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHUN9_002132"
     CDS             735..1040
                     /locus_tag="ACHUN9_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695526.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUN9_002133"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUN9_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0695527.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHUN9_002134"
     CDS             1788..2534
                     /locus_tag="ACHUN9_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0695528.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHUN9_002135"
     CDS             2534..4630
                     /locus_tag="ACHUN9_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0695529.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHUN9_002136"
     CDS             4676..5815
                     /locus_tag="ACHUN9_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0695530.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHUN9_002137"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHUN9_002137"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0695531.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHUN9_002138"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHUN9_002138"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0695532.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUN9_002139"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUN9_002139"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0695533.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUN9_002140"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUN9_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0695534.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUN9_002141"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUN9_002141"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0695535.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUN9_002142"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUN9_002142"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0695536.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHUN9_002143"
     CDS             complement(12873..13730)
                     /locus_tag="ACHUN9_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0695537.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUN9_002144"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUN9_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0695538.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUN9_002145"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUN9_002145"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0695539.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUN9_002146"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUN9_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUN9_002147"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUN9_002147"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0695540.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUN9_002148"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUN9_002148"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0695541.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4546 a         4915 c         4782 g         4272 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accgcttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccaa ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaact
//
LOCUS       DAZOYS010000072        18351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOYS010000072 DAZOYS010000000
VERSION     DAZOYS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.072"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(147..1256)
                     /locus_tag="ACHUN9_002149"
     CDS             complement(147..1256)
                     /locus_tag="ACHUN9_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR0695542.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            1414..2598
                     /locus_tag="ACHUN9_002150"
     CDS             1414..2598
                     /locus_tag="ACHUN9_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0695543.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2598..3110
                     /locus_tag="ACHUN9_002151"
     CDS             2598..3110
                     /locus_tag="ACHUN9_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR0695544.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            3165..3530
                     /locus_tag="ACHUN9_002152"
     CDS             3165..3530
                     /locus_tag="ACHUN9_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695545.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            3539..3694
                     /locus_tag="ACHUN9_002153"
     CDS             3539..3694
                     /locus_tag="ACHUN9_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR0695546.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3681..6488
                     /locus_tag="ACHUN9_002154"
     CDS             3681..6488
                     /locus_tag="ACHUN9_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0695547.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6501..6989
                     /locus_tag="ACHUN9_002155"
     CDS             6501..6989
                     /locus_tag="ACHUN9_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695548.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(7025..7612)
                     /locus_tag="ACHUN9_002156"
     CDS             complement(7025..7612)
                     /locus_tag="ACHUN9_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0695549.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            7717..8703
                     /locus_tag="ACHUN9_002157"
     CDS             7717..8703
                     /locus_tag="ACHUN9_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695550.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTDSY
                     MWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGDIF
                     ANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNN
                     WQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            8705..9232
                     /locus_tag="ACHUN9_002158"
     CDS             8705..9232
                     /locus_tag="ACHUN9_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0695551.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(9261..9794)
                     /locus_tag="ACHUN9_002159"
     CDS             complement(9261..9794)
                     /locus_tag="ACHUN9_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0695552.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(9797..11767)
                     /locus_tag="ACHUN9_002160"
     CDS             complement(9797..11767)
                     /locus_tag="ACHUN9_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695553.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNTTNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNQASGALQKNQNGADIPGKDTFTKNIGACRAYSSWLNIGGDSQVWTTA
                     QFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIR
                     VTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAV
                     GWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEAD
                     WSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTG
                     SVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            complement(11770..12300)
                     /locus_tag="ACHUN9_002161"
     CDS             complement(11770..12300)
                     /locus_tag="ACHUN9_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR0695554.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(12293..13189)
                     /locus_tag="ACHUN9_002162"
     CDS             complement(12293..13189)
                     /locus_tag="ACHUN9_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0695555.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(13193..13543)
                     /locus_tag="ACHUN9_002163"
     CDS             complement(13193..13543)
                     /locus_tag="ACHUN9_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR0695556.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(13540..14121)
                     /locus_tag="ACHUN9_002164"
     CDS             complement(13540..14121)
                     /locus_tag="ACHUN9_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0695557.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(14118..14753)
                     /locus_tag="ACHUN9_002165"
     CDS             complement(14118..14753)
                     /locus_tag="ACHUN9_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR0695558.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(14746..15213)
                     /locus_tag="ACHUN9_002166"
     CDS             complement(14746..15213)
                     /locus_tag="ACHUN9_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695559.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(15285..15758)
                     /locus_tag="ACHUN9_002167"
     CDS             complement(15285..15758)
                     /locus_tag="ACHUN9_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695560.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            complement(15755..16303)
                     /locus_tag="ACHUN9_002168"
     CDS             complement(15755..16303)
                     /locus_tag="ACHUN9_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0695561.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            complement(16284..16586)
                     /locus_tag="ACHUN9_002169"
     CDS             complement(16284..16586)
                     /locus_tag="ACHUN9_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695562.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            complement(16580..16777)
                     /locus_tag="ACHUN9_002170"
     CDS             complement(16580..16777)
                     /locus_tag="ACHUN9_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR0695563.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            complement(16777..17271)
                     /locus_tag="ACHUN9_002171"
     CDS             complement(16777..17271)
                     /locus_tag="ACHUN9_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR0695564.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(17373..18173)
                     /gene="gpM"
                     /locus_tag="ACHUN9_002172"
     CDS             complement(17373..18173)
                     /gene="gpM"
                     /locus_tag="ACHUN9_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR0695565.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            complement(18219..>18351)
                     /locus_tag="ACHUN9_002173"
     CDS             complement(18219..>18351)
                     /locus_tag="ACHUN9_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR0695566.1"
                     /translation="MRRLMKDEPEYNRIATYQSINDAYVVEDYGKCAMVTGLKFADS"
BASE COUNT         3964 a         4811 c         5056 g         4520 t
ORIGIN      
        1 cccctgatgt caggtaggaa aacaccatat gtttctgctc tgtttttcct tagaaatagg
       61 catccttgct ggaacaaagc gagcagctat attgactcta tgttgtgagt gtgttgatcc
      121 gttctgagtt aatacattta agttgctcac tctgtttctg ctatccattc cgggattttt
      181 gcctcaagct caagctgcgt ggtaaagccg ctgttatcaa tggtgtgctc ggcttttgca
      241 ataatccagt cctgattatc aatctcgctt ttaaatcctg ttaccgtgcc atgcatttcg
      301 gggtagagtt ctgcacgtcc atgcgccagt gtgatggaga atgatgcggc tccgcgttgt
      361 agctgctgcc actttgccgc cgctgcacgt cttgctgcct gctcgttctg ataagttttg
      421 cgtaacacaa acacgttgcc ttccgcacct tccatatagt cgccttcccg gctgctgctt
      481 ttctcctttt ttggttttga cggtttgcgg cgtttcacgc tgactttttt ctttttcccg
      541 taattaagat caagccagta agcgcgtacc cccgtatacg cttcgcggtc agcaatgcgg
      601 aactgatggc gatcgccgct gctgcgtgtg atggcgaacg agggcaacgg ctggccctgt
      661 gcgttcacgc caccaccggg catgatgaat aacagattac cgctttttac cgtggtgatt
      721 gcgcccagca tttccgccat gcgcgtaagg aaggacatgt cgctttcttc ggtctggtcg
      781 gcgtggtcga tttcgatatc catcagcatt tcgctgattt gcggtttcag accataccga
      841 tgagcgatgg cggataccac acgctcaacg gtcacatcat gccaggacac ctcacgttta
      901 acgttaaatt cagcccgaaa atctgcgctt ctggctgaaa cagtcagcct gtccggcggt
      961 ccttcgtgag cgatttcatc aacaatgtaa gtgccttttt ctgtcagcgg ttctcccttc
     1021 cagccaatga gaaccgtcag gcgcgcgccc cgtggcggta gctgcaactg accatccgca
     1081 tcatccagcg tgatggtgag ctggtccgcc tcaaatcccc ggttgtcggt cagtgacagg
     1141 ctcatcaggc gctctgccac gcctgacagc gttttaccct ccgcgagaat atcaaaatcc
     1201 ggcattttca cggggtctgt gccctgactg agcaattgca tggtggtgtc ggtcatctgc
     1261 tccctccctg tgtggcatgg tcgcatgtgc gtgcggaggg ggttactgct ttttgttgtc
     1321 gccgtggcgg gagaatggcg caggggtgag attacgcgcg tggtgggtga tgattgttgc
     1381 cgaatcattt aacggataca aggggttgaa gctatgagtg aaactcgttt tcatggtgcc
     1441 cgtgttacgg aaaataccga cctggtaaca gcgattaacg atgttgattc cagcgttatc
     1501 ggtatcgtgg caacggcgga tgatgcggac gcggagctgt tcccgctgaa caagcccaca
     1561 ctgctgcccc gcgtcaatga cgtgctggga aaatgcggaa caacggggac gctttatcgt
     1621 gcgcttaagg ccatcgcaga ccaggtgagc acaaaggtga tcgtcgttcg cgtggctgaa
     1681 cacaaagaag aagacggaaa aacgcaggat caactggtta tcggtggttc tgaatctgac
     1741 ggcagctata cggggatgta tgcgctgctt gttgcagagc aggatgaaag catcggatac
     1801 cgtccgcgta ttctggccgc gccggagctg gacacggagg cggtgacaaa atccctgtgc
     1861 gtgattgcgg gtaaactgcg cgcgtttgtg tatgcctcat gtcatgactg taacacgatg
     1921 gctgaggcaa ttacctaccg ccagaaattc aacgaacgtg aggtgatgct cttatggccg
     1981 gacttcatcg cctacaaccc gaaaagtggc aaaaacgaaa cgttccccgc gcctgcctat
     2041 gcgtgcggcc ttcgtgcgta cattgaccat gagcagggct ggcacaaatc gctgtccaac
     2101 gttccggtta aaaatgtgct ggggatgtcc aggcatgtgt tctggtcgtt gcaggccgaa
     2161 gacagtgatg ccaacagcct caacaacaaa gaaatcacga ccattattcg tcgcaacggg
     2221 ttccgcttct ggggcaaccg cacaccggaa acgaacgcct acatctttga ggtgtatacc
     2281 cgaaccgcac aggtgctggc tgattcaatt gcggaagcgc agtttgaaac catcgacagt
     2341 ccactgacgc ctgcgaacgt gaaggatgtt atcggtgcca tcagggcaaa actggattca
     2401 ctggttactg ccgggaaact gattggggcg tcgtgctggt atgacgtggt ggataacggc
     2461 accacgaatt tacgtcaggg gcgcgtgcgt attcgctaca aatatacgcc tgttcccccg
     2521 ctggaagaca tggagcttta ccagtcgttt actgatgaat tctttggtcc cgcatttgcg
     2581 gtgctgggag gtgcctgatg gctgtaccaa aacatcttcg cttttttacg ctgtttgtgg
     2641 atggtgaaaa cgaagtgggt aaggtgacgt ccgtcactct gcctaagctg acgcgcaaaa
     2701 ccgacagcta ccggggtggt ggcatgatgg gagcggtaag tattgatctc ggcctggatg
     2761 actccgcgct tgatgcgagc tttgtcatgg ggggcgcagt tcgtgagctg ttccttaagt
     2821 atggcggcac gattgacggt acgctgctgc gttttgcggg tgaatactac accgacgctg
     2881 aaagcgacct gtatgaagtc gaaatgcgcg gacgtgtgac ggaaattgat atgggggaag
     2941 ccaaacaggg cgaagccaca tcacacactt acgccattaa aaacacctac tacaagctga
     3001 gtgttaacga tcgcccgttg tgggagattg acctgctgaa cttcatttac cggaaggacg
     3061 gcagggacat tgtgcccgat cgcatccgtt ccgcgctcgg gcttggctga taagtaatat
     3121 gcaggcggcg cagtgcgtcg cctctgactg aaaggagttt cctgatgaaa gagacgaaaa
     3181 acatcgatac cgaaaacacg gtcgttgctg acactgtgaa agaaaccagt gagcgtggcg
     3241 taaaacttac ccaaccaatt gagcgaggcg gcgaaaaaat cacgtatgtg gagatcaccg
     3301 gggctattga gcaggctgga tctctgcgag atttgtcgct gtctgatgtg ctgaatttga
     3361 aagcggaatc catgtttacg ctgctgtcac gcgtgacatc accgcgactg gatgaagtga
     3421 cgatcaaaaa aatggcatcc cgtgacttta ttcagttatg tgtggttgcc gtaaattttt
     3481 tgagcggtgc ggactctggc gagaagaacg aacaggcgac ggaagcctga tcacggttgt
     3541 gtgctttgag cacatagaag actttgtggc ggatattgcc gccattttta actggtcgcc
     3601 cgccgaaatc ttcatgatga cgcccggcga agtggttagc tggcgtgagc gggcggcact
     3661 tcgcagcggg aatgcagaca atgaagactc ttgatatccg ggtcgctttc agcgccgttg
     3721 acaggctgac ccggcctgcc gaaaacgccc gccgcctgat ggggcagttt ggtgactcca
     3781 tccagcgaac gcagggggcg atcaaaaatc tcgagcgtca ggcgcgttca tttgagcgcg
     3841 cccgtgacgc tgtcagtaaa gcggatgcgg gcatcgtgaa agcacgacgc cagcttaacg
     3901 cccttaatca gttacaacgc acgggtacag tgctcagcga aaaacaacaa aagctgatgc
     3961 agcagttaag cacccggctt gaacgcctga atgaatcgcg cacacgggaa attcagaaaa
     4021 tgcgggaact tggcggagag ctgaaacgcc acggcatttc cctgacaggc agcgataaca
     4081 ccatccagca ggccatcaga cgcaccggac aatacaacaa ccagcttgaa cgcgaacggc
     4141 gggcgcttgc gcgtgtaacg caggcgcgtg agcggtattc acgcgcgcag gaaacagcgg
     4201 gaaaactgaa aacaggtggt gcgctggcaa caggtgcggc ggcagcgggc ggctatgctg
     4261 ccgggcgttt tttgcagccc gctatcgggt tcgggaaaga gatgtcccgt gttcaggcac
     4321 tgacgcgaat cgataaaaac agcccgcagt ttaaggcgct gcgtgagcag gcgttaaaac
     4381 ttggctctga aacgcagttc accgcaggcg atgccgccag tggacaggca tttcttgcaa
     4441 tggctggttt cacaccgcag gccattcagg ctgcgcttcc cggcgtgctg agcatggcaa
     4501 cggctggcgg tatggacctc ggcgagacgg cggatattgg ttccaatatc ctgacgcagt
     4561 tcggcctctc tgctgaccag atggaccggg tcggtgacac acttaccgca gcgtttaccc
     4621 gtaccaacac tgaccttcgc gcactgggcg aaaccatgaa atatgcaggt ccggtggcgg
     4681 gtaagctggg aatatcgctg gagcaggccg cagcgatggc gggcgtgctg gcgaagatgg
     4741 gtatcagggg gagtgatgca ggtacagcgt tgcgtaaaag cctggccaat ctgtcatcac
     4801 cgccaaaggc agcggcagaa gcactgaaag agctgggggt gtcagttgct gacgccaaag
     4861 gcaaaatgcg cccgatggag aatgtgctgg ccgatctcta taaagccacc cgcaaatacg
     4921 gggaggttga ccgggtatcg ttctttaagg acattgccgg agaagaggct ttcacatcat
     4981 ttatgggcct cgttgatgcg gcaggtgacg gcttcttagc caaactgaga aaagaacttg
     5041 aaggcgcgcg cggtgaggca gaacgcacgg caaaggttat ggccaataac cttgatggca
     5101 acctgaaatc actcggcagt gcatgggaag gtctgcgtat tcgcatcagt gatctggttg
     5161 acggtccgct gcgttctgtc acgcagtggc tcacgcgggt tgtctcaaag gtgacggcgc
     5221 tggcgcaggc ccacccggca ctgacgcgcc agttactgat agcgggaggc gcactgctgg
     5281 cgacaacagc aacggttggt tcgttgtctc tggttattgg ggtgcttcac gggaagctgg
     5341 ctacgttgcg tctcggtttt tctctcctga ccgaatcaat gaatgttgtc agggttctgc
     5401 ctgcattatg gggaatggtg acgggttccg tttctttact tggtggcgct atcggggcgc
     5461 tgttcagtcc ggttagtctt atcgtggctg cgcttgccgg agctgccgtt cttatctgga
     5521 aatactggga tcccatcagg gcattttttg ccggggtgtt cagcgggatt atggaaaggc
     5581 tgaccccgtt gcgcgaaacc tttgaacggt ttggccctgt ttttgatgtc gtgcgcgatg
     5641 ggattattca ggtctttaac tggtttaaat cgctgctgtc accgatggag tccagcaagg
     5701 aaacgctgga taaatgtgcc agtgctggcg agatattcgg taacgttctt ggcggtgcgt
     5761 tacaacttgt tctgacacct gcaaaaatgc tactggatac gctggcgtgg atacttgaaa
     5821 aacttggcgt ccttccggat gaagcggaaa gggcgcgcaa gaaaatcgaa gacgcacagc
     5881 gtgcggccat tcttcaggac aaggttgcct tgcttcaggg ggacctggcg aaaatcaatc
     5941 cgccgaagcc tgtggaaaat ggcaatggca ccggaggtga taaacccaaa gacaacaaac
     6001 cgctcacaga cagcaatacc gggacgctac gcagactcag caaaattgct gataacacag
     6061 gtaagctggt tgatgagacg aaaaaacgca ttggccccgg cgatattgtc tttaagaacc
     6121 tgccccgcgc acttgctgtt cgtggggagt ggcaggagcg gaagattgcg caggtcagta
     6181 agcctgcccc cgcaattaat atcacacccg tggtcccggc tccgctgcct ccggcgctgg
     6241 tccctgttgt tgcggccagc tcccgcccgg tggcggaggc catacgctct ccagtggcat
     6301 cagttcctgt aacttcccgt aaccgggagc ctgttgcctc cggatttggt ggtgaaattc
     6361 atgttcatct gcataacgtt gttacgcaga atccccgcga actggcgaaa ctggtcggtg
     6421 aaatggtcag ggcagaaatg gaacggcgcg cccgtgccgg gcgtggcagt ttttacgata
     6481 aagattgagg agtcatggcc atgatgatga tctacggcat gtttgttttt gagctgcgta
     6541 cattgccgca tcagcagtta cagcaaaaca aaagctggcg gcatgtgaaa aatgaacgcg
     6601 ttaaccgttc ggcaagctgg cagtatatcg gtgcaggtga tgagcgcatc gttctttctg
     6661 gtgtgcttta tcctgaaatt acaggtggcg aagtgtcgct gtcgctgctg accacgcagg
     6721 cgtatacagg acgaccctgg cctctgattg atggcgtcgg gcagatttac ggcatgtatg
     6781 tcctgaccga aacgaatacg acccgctccg agtttgatcg ctacggtaag gcgaaaaaga
     6841 tagaattttc actgactctt gaacgctgtg atgaggattt gcgggagcgc ctgcaatcct
     6901 catcgttcag cgatatgctg tccggcttca aagataaggt aacatcatcc cttaacagcg
     6961 cggccagttc agttaaaggg ttgttctgat ttaacacaaa aaccgctaat ggtcagatta
     7021 gcggttattt tgtttcctga ctcttctcta ttgttccgat tgattctcct gcggggtggt
     7081 aacgataaat cgtcgatata ccaatgccgt aaattattgc cagttgtttt ctgtcgtgcc
     7141 cgtttttgat cagccttgct atttgctcat gctgttcttt tgtcagcttc ggtcgacgtc
     7201 cgcctgtgcg cccccgtgcg cgtgctgccg ccagtccggc cagtgtacgt tcaacaatta
     7261 attcacgttc catttcagcc agggcaccca tcacgtggaa gaaaaaacgc cccattggag
     7321 aagatgtatc tatgctgtcg gtcagactgc gaaaattaat tcctcgctcc cgtagttccc
     7381 cgacgagaga aatcagatgt ttcatgcttc gcccgaggcg atccagtttc cagacaacca
     7441 gcgtgtcacc tttttgaagg cgctttaaag cgcgttttaa tcccggtcgg tctgtctttg
     7501 tcccgcttaa tttatcttca aatatttgtt cacatcctgc acaaacaaga gcgtttcgtt
     7561 gcaggtctgt attctggtca tttgttgata cccttacata gccaatcagc acgctgaatc
     7621 tcccgtccaa aagcgcaaat catgccatgc aggccggaaa cggccattat ctaaaacctc
     7681 ggtttacagg aaacggtaaa caaggctggt aacgccgtgc aaaaaaatgg cgatacgttg
     7741 tccggtggac ttacttttga aaacgactca atccttgcct ggattcgaaa tactgactgg
     7801 gcaaagattg ggtttaaaaa taattcggat gcagacaccg attcctacat gtggtttgaa
     7861 acaggcaaca acggcaatga atatttcaaa tggagacacc gcatcattgg cacccggcct
     7921 aaagacctga tgaatcttaa atggaatgct ttgtctgttc ttgttgaagc ccttttcagc
     7981 agtgaagtga aaatatcgac agtcaatgca ctgaggatat ttaattcatc ttttggtgcc
     8041 atttttcgtc gttctgaaga atgcctgcat atcatcccta cacgagagaa tgaaggggaa
     8101 aatggtaata tagggccact acgccccttt acgcttaatc tcagaactgg ccggataagc
     8161 atggggcatg gtcttgatgt tacaggggat atatttgcaa accgttttgc aattaacagt
     8221 agtaccggca tgtggattca tatgcgtgac cagaatgtta ttttgggacg taatgcggta
     8281 tccactgatg gtgctcaggc tttgctccgt caggaccatg ccgaccgcaa atttatgatt
     8341 ggcggtctgg gaaataagca atttggcatc tacatgatta ataactcaag gacagccaat
     8401 ggcaccgatg gtcaggcgta catggataat aacgggaact ggctttgcgg ctcgcaagtt
     8461 attcccggca actatggcaa ttttgattcc agatatgtga aagatgttcg acttggttca
     8521 cagcaatatt atggagtgaa caactggcaa acatggaatt ttcagtgccc gtcaggctat
     8581 gtactgacgg ggattaacgt acaggatacc ggaaaaaact ctgccgataa tattgcaggc
     8641 gttcattaca ggccagtgca aaagtatata aatggcactt ggtataatgt ggcgagtatt
     8701 taatatgatg cacttaaaga acataaaagc aggtaatgct aaaacagtgg agcagtatga
     8761 gttaacaaag aagcacggag tcatctggct ttactctgag gacggaaaaa actggtatga
     8821 ggaagtgaag aactttcagc cagacacaat aaagattgtt tacgatgcaa ataatattat
     8881 tgtcgccatc accaaagatg cctccacgct taaccctgaa ggttatagcg tcgttgaggt
     8941 tcctgatatt acagctaatc gtcgtgctga tgattccggt aagtggatgt ttaaggacgg
     9001 agctgtggtt aaacggattt atacggctga cgaacagcaa caacaggccg aatcacaaaa
     9061 ggccgcattg ctttccgaag ctgaatcagt catccagccg ctggaacgcg ctgtcaggct
     9121 gaatatggcg acggacgagg aacgcacacg actggaagca tgggaacgct acagtgttct
     9181 ggtcagccgt gtggatacgg caaagccaga atggccacaa aagcctgagt aaaaattaag
     9241 gcccgatatc gggccttgtt tcattctggt tgttcgggaa acgttactgg caggctggag
     9301 gtgtctgtgg attcgacctt ctgcgcatag agcatccact cggttaattt ttgtttattc
     9361 tcgtcggaaa taatgcccag ccgtagctgt gagtcccata gctgggtttt atccctgacg
     9421 agctgtagca ggctttgctt ttcattttcc gcttgttgcc tctgctcttc cccggtataa
     9481 gttcgcttta tcactacgcc atctttgaac atccatttac ctgaaatatc agcccggcga
     9541 tttgctgtaa tatcaggaac ctcaacgacg cttgcgcctt ccggattaat tgctgaaaca
     9601 tccttttcaa tacaaataat aacgccgttg tggtcataga ccattttcaa cgtatcaggc
     9661 tgaaagttct tttgttcctc ataccagttt ttcccatcct ctgtataaag ccatttgatg
     9721 ttaaattgtt tcgttagctg gtattgctct tttgtttcag gattaccagc agaaatgttt
     9781 tttaagtgca tcatcgttaa atactccccg cgttatacca cgtcccatta atgcaatact
     9841 gaattggcct tgcctgagtt gtatcaatta gttcatctct gtttgagtta acggaaccgg
     9901 taacaacata acctgacctg tcagaccatc cgggaccatt ccatgtctga acagataaca
     9961 gaccaccaag gcgaatacct gtaataaacc ttgagttaca ttctgcctgc gtatatgcac
    10021 caacatctcc cgcagagggt ttgcgggttg tggtgtaaaa ctctgaccag tcagcttcaa
    10081 agccataacc atcacgcgct gaacgataaa aaataccgcc gttcctgtaa ttcacgcgga
    10141 actgtacagc agggcagctc cccgtattca tattgaagtg gaggattaat gtcgatgcgc
    10201 caccaatgtt tgcgttatag accccgctat tccagttcca gccaacagct ttatcatttc
    10261 cgacagtgct tcctgtttgc cctaaagcaa atgcaggttg ttgatttttc gtgttgtagt
    10321 ctcgtcgcca gccaggagcg taagcatcac catgattaat ataagtgaat tgagcgttag
    10381 taattccgcc accgctggac gtgctcggcg tggtaacgcg tatggtcatt gcgccgcgag
    10441 tgccaataac ttccaccaca gcacctgcaa gacaaatatt tccgcaacct gtatctgtaa
    10501 tgaccttatt atttgcataa gcccatgagc ctttgcacat ccagtaagga tggttaaatg
    10561 ctccctgact ctccagccac gaaataaatt gcgcggttgt ccagacctga ctatcgccac
    10621 caatattcag ccatgagcta tatgcgcggc aggcaccaat atttttggtg aaggtatctt
    10681 tccccggaat atctgcgccg ttctggtttt tctgtaatgc gccagaagcc tgatttaccg
    10741 tttcctgtaa accgaggtat tcgataacgg cggcaacggt cgatttcgca agaatatccc
    10801 gcccgacttt tgtcagggtt gccaggctgg cgacatcatt ccccgtaaaa tacggaaacc
    10861 tgtctgccgc agtagcaagc cccgccagcg ccgtcagagt ggcatctttc ggttgcttac
    10921 ccgcaagcgc gttagtcatg gtggtcgcaa aattcgggtc gttgcccagc gccgccgcca
    10981 gctcgttcag tgtgttcagt gcatcaggcg aagagtctac aagtgcggca atcgcagcca
    11041 taacgaaagc cgtgcttgcg atttgggtat tattcgttcc ctgtcgcgca gttggtgttg
    11101 ttggcgttcc ggtcagtgcc gggctgttta atggggcttt cttgttcgtt tcatccatta
    11161 ctgccttaac ggcttttggt gtcgctgcca gcgtttcaga cgtgctgttg gtggtgttgc
    11221 tgagctggac aaggcctttt gcggtcagcg aggcgtccgg gtgacgtcgt gactgttcat
    11281 gctctttcag tttgtcatcc acgtaattca ctgtggccat caccatggtg ttatccacgg
    11341 taagcgccac agtggcagtg ctggatacgg tcagaatggt gcgaaatgtt tgtgcacgtc
    11401 cggatccttc ggcaacggtt ggcttgtaac tttcggcagt attgcctacc gcgatcaaat
    11461 cgccgtgctc atcaaacaca ccaatttccc ggatccagaa tccgcccgtt tcaggaggaa
    11521 taaccagctc cgcaataatg tggttctgat gtgttgcgtc cagggtgacg cgattaacgg
    11581 tatgtcgcca cacctcatgc acaagacggg tctgcttact gtctggcgtg ggtaaagtac
    11641 cgccgccgtc acccacggcc atatgtgtca ggcggacagg tttcccatct ggcgcggttg
    11701 cctgcgctaa tttttttgca cccgtatcgg tgataatggt tttaaatttt cgtgttgtgg
    11761 tactcatgct taatcgtccg gataaatggt aatgacttca ccgtcgtaag ttgctgctgc
    11821 cgcgaaaata tctccctgaa tctcctgaat gatattcagc cctgtcatgt ggcggctgac
    11881 cgggcgggca tcagcaatca accgctccat ttccagatac atttcctccg tcacgccact
    11941 gtccagcgtg ccaacatcaa tcttgaatgt tccgggttcg ccgttgaact cccaccactc
    12001 agacacgcga atgaggtatc ccagcggctc aatggcccgt cgcagggcgc tgatggttcc
    12061 tttgtgtcgg tgtatcagcc atgcatcacg aatcacctgt cgctttgtct cttccggcca
    12121 gttgcgatcc cagcggtcaa cggaaaacgc ccaggcgaga taaggcagca gatgcaccgg
    12181 gcaggtgtcc ggcgaccaca gcgtgttgag gtctgccgga atgtctgtaa tgcgtgttcc
    12241 gacggcttcg gcacaacgca tgaaattgct ggctgatggt ggtaacagtg aattactcat
    12301 tacgcccacc ttcgctgatg gtgaatgact cacagcgcgc cgcctgtatg tcgctgatgg
    12361 ccatattctg tgtgggttcg attatctcca cgcgttgcac accgtgcaca tgcagtgcgg
    12421 cagcaatggc ggacaacgcc acgtcctgac cgataagccc ctgctcagcc agccacttcc
    12481 tgaacgacga ttcagccgca gccagaatag gttcggattc cggaccgggg taaaagtaca
    12541 gttttgcatt cagccgccat gtcacgattc tggcactctg tacggtcagg cggtcggcca
    12601 ccgggcgggt atcctctgca ttcagaacgg cgcgaacggt attaagcaac gcctccgttg
    12661 ctgtgccgtc gccctcagtg gacaggatgg aaaccgtcac atttgccgga gacggactga
    12721 tggcccgcgc atcgcgcacc agaccgctgg cgctgcgggc aaaatactcg tatgcgcctg
    12781 acgggcctgc aacactcagg ccgtcatacg cccgctgcgc ccgcagtctc agcgaggtgt
    12841 cgttctccat caccacgtcg gtggtatccg ttgccggagt gataaccagg cgctttgtgt
    12901 tcatattgcc cgcgaggttg tccaggtctg ttccggcgct gtggctcaac atgcaggcgc
    12961 gtgcggcctc attgacccgc tggcgtaaca gcatttcacg aaacgacatg gtttgagcga
    13021 taacgttcag gggttccgat tccagttcca gtgcggcgga gacgacttca cgctgttcgg
    13081 cgggataagc tgcaatcatc atggcctttg tgtcagccag aattgcctca aagtcaggct
    13141 ccgcgatgat ggcgggttcc ggtaactggg aaaggtcaac ggcaggcatg atttactccc
    13201 tcagcgtgat ggttaattca acattctgca tggtctgcat gacagtgccc gacagcgtca
    13261 ccccggcgtg gcctcctgcc ttccagacaa cgtcgatggc gttcagggca atgcgtggtt
    13321 cccatcgtgt cagtgcaatc acggtagcac tcatgcattg cagacgcgtg gtgttattca
    13381 tgggttcgtc aatcaaatca ggcacaaggc tgccatattc ccgtcgcata acccggcttg
    13441 ccagcggggt ggtcaggatg tccctgactg actgtttcag gtgcgccata tcgttcaggt
    13501 ttcccgtccc gtccgggttc attcctgtgt agcgggttgt cactgcgggc ctcctgtcga
    13561 atcgctgcca cctttaacgc caccgtgctt atgcgtatgc actgtgatgc cgtttgaggt
    13621 gaaatcgccg ccgctgtgcg tgatattgcc gctcatcgtt cccccttttg tgacgtcaag
    13681 cgtcgctgtt ctcagaaggt ctgtgcattc cacgacaggc gtgttcagtg tcacgctgat
    13741 ggatgcctgc aaagtggccg ttttcatgcc gcttgcgctc agtgcgcctg cgtccgcgtc
    13801 gtagcggaac accgcgccgt ccggcgcgct gaccacgatt tctttcaggc ttttgccggg
    13861 tgccggaatg gcatcactcc acaggctgcc gattatcatg gcggtttccg ggttgccgcc
    13921 gatgcaggca attgccacct gttcgcctgg tgatggcgga aaccacacgt tgaagtctcc
    13981 cgcgcgcgtg gtgttccagc gcagccagcc tgtttccagt tcgccgctgc gaacgcgcac
    14041 gcaccaggat tcctcatcaa cttcagagat gatcccggtg cggatgatat tgctcagcag
    14101 tcgcatgagt tctgcgctca tcgtacagcc tccgcaatcc ggcccagcac cgtgttataa
    14161 atcaggcgct catctgcctg gctgataccc agcagctcac gtaccgggta atcggtgaaa
    14221 atgcccggcg caacctgatc gcgctcaccg aactgatgaa cgcgtgcaat acgtgcggcc
    14281 acgccgctgt aacccaccgt cacaccggaa gcatctgcac gggctttcag gtagcgggcg
    14341 gtgcgcagtt ttacgaacat ggagacgcgc ctggtgctgt cctggttgat gcgccgggtg
    14401 cgtatttcca gaaaacggtc gatgtcatcc cggtaaaacg tgcggatatt gtttttatcc
    14461 tcatcccacc cggtgatggt tcgcccgtat ttccccgtgt cgtgatgcca gtttttcagc
    14521 gtgcgtgctt cgttattcca gataaagcga atgcgctcct gtatccgggt tacgcggcgt
    14581 ctgcgtggtg tccatgcggt cccgtccggc gctttctgtg accggatacg cgcctgctgg
    14641 gcgcggcgta aatcctgtgc cagctttctg gcgatgttat taatggcctg ctgattcagg
    14701 ctgtcgcgga tagcctcaaa ggtttcatcc acgcgggtga atgccttatc catcgctttc
    14761 cccccacgtc acatcctgga atacatgcga ccagtcgcct tcggaagagg gcaggcgggg
    14821 ttttggctcc ggcaggtgtt ctgcctgcgg tgtgccctga ctgctgcgcg tgatgcgaac
    14881 gcgttcccgc agggggagcg taaacaggag atcggcgctg tcatcgtcat tgataacggc
    14941 ggaaaatttg atgtcctgat tacgctccgg attgagcaac aactgtggct gattttcgga
    15001 taaccacgcc agcagcggca gcgtgaggtc gtccagctct ccggcgtaat ccatgacaaa
    15061 catcactatc tgatagcggt aaacaaacga tggggtttct ccggtcgttt caatgttgcc
    15121 gctctccacg aaaatggtga atttttccgg gttggcatga caccaccggc atgaacgggt
    15181 catggcttca cgcagggaat cagttttcag catggttgtt gtcctcgttg ttcagtcgtt
    15241 gcagcctgcg ctgttccagt aattcaatgg cccgcttatc ggcgttacag gtttccagtg
    15301 cgtccatgag gctgtcgctc catatcccga tgtttcccca tgatggcgtc accggaaacg
    15361 gcgggggaag tacgggggcg gtcagccctt ccgggatgaa acggaacgac ggcggcggcg
    15421 cggacgcgtt ctgcgtgctg gtgcaaccgc tcagtaaagc gagcgtcagg aacagcgcgg
    15481 gcacatgcat ctgtcgtgat atcgttgcgt agctgttcac gtcggatttc cccttcctgt
    15541 tgtcggttct ggcgcgctgt catgacgtca cgcagtacac cggatgcggt gctgatgatg
    15601 gtgccggcct ctttcagggt ccggctgtaa tcgtccagtt gcgcctgtgt ataacggttg
    15661 tgtccgtctt cctgccccag tctgaatgtc tgccagagtg ttatggtcag cagcgtgagg
    15721 acggtcagtg ccatcatcag gatgtaacgc actttcatga ctggcctccg gtgtcacgta
    15781 agcaccatgc acgaaaatca gtacgacggt taaccagtcc ctgtgagcgt tcgccgccgc
    15841 tgttcacgaa gtcagtcagt ctgttgcaca tgtcaggcca gttgtgcgcc tgtgcatgtt
    15901 tccagatagt ggtgcggtgg tagttacggt tcttatccct gaaccacata agatttctgc
    15961 atccaagatt aaaggcggca tctgtcattg cctcaaacgc ggactgtggc atggcgttgc
    16021 cgctgaagtt ctgatttatg cagttttctg catgttgcat atcattaacc cagcgcccgg
    16081 cgacttccgt ttcggtgtac agacgatttt ccacatgccc ggtcgagccg caccccacgg
    16141 tcattacccc ggcaatatcc cggtaaggcg ttgcgcgaca gtcctcccat gtggcaatgc
    16201 ggatttgcgc ttccgggctg gtccggagtt tgtgaggggc aatggagaac cccagcgcca
    16261 caatggcggc tacggcgtaa cgtttaacgg gcagctttat catgtgtatc gttatcccgc
    16321 agaatgttca gagcctgacg ctcactgtcg tgaagtggcc gtgcgtctga ctgcgccagg
    16381 atactggcga tgagttcatt gcgacgctgc atggcggttt ccatcaggcg gcgatgtcgc
    16441 caggcatgta atgcagacag agaaccgagt aacaacccgg caagggcgat tttttcactg
    16501 atggtcatca cgcctattgt ggtggctgtg actgatgccc agaaggtgat ccagtcactc
    16561 acccgctgaa aaaaacctgt taccatagct gtatcatctc tcgttgtttt ttcttctccg
    16621 gctccggcat ttccacctcc tgtccggctt ccagaaatac ctgtcggcag agtccggggt
    16681 tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg aaacagagcg
    16741 aatccacggt gtcgccttcc agtgtcttca ctttcatcag cacaactccg caaagattcg
    16801 cgggcggcac agaatgtcag agatggccca gctcacatcg cgccacaaat ccgatgtctg
    16861 tatatccagt gcgtccgccc ggcggtcgcc cttgtccgtt gtgtccgcat cgcggtaacg
    16921 ctccagaatc agggcgcgtg tggcggtata aacagcattg cgccagtgcc agagattgac
    16981 gctttctccg ttaattacag gtgccggaac atcggccagc gtctgatggc cagccgcctg
    17041 ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacggccc cggtggcggt
    17101 atgcagcagg cgggaggtgg tcacgcggcc cggcagtcgt accgccagac gcagctcgcg
    17161 cagcacaata tccggccaga atgcacccgc tgaaatacgg gtatcgccat cttcggtgtc
    17221 ggtgatgtcg tcctctgcgg gtccgggttc agttctggca accatactca tggggttcac
    17281 tcctgaaaaa atcgggcggt gggtgcgcgg tgtaaacggt cacggagcca aaccggaaca
    17341 ccgcgcacgc cgcccgctga cggggtcagt cgttaaccgc gcttcgcctt ctgcgtcgcg
    17401 gtggtttttc gtgttgcagg cttccgcgtt gtctttttac tttcgctgct ttcgtcctgc
    17461 gcctgctgtg cgctggcgtc ttctggtgcg gctgcggaat cggctttttt cagggcgcgg
    17521 gaaagggttg caatctcgcg tttcacacct gcgttctggt tcagatgcat cgcttcgcgc
    17581 agcagcttca gtgatgaggc catgctgtcc gcatcgctca ggccacggcg ggcaaaggcg
    17641 cacgccttgc ataatttggc gcgcacttcg tccggcatat cctggttggc gacaatttcc
    17701 caaagtgtgt ccagtggttc gataaagacg gacaaatccg cgtcggcatc cgtcccggcc
    17761 tgcgtcagta ccggattgca gatttcttcg gtcagcactg tggcagcagt acggccaaag
    17821 ttatccggca tgatgaggtt gtgacggacc gcatacgcac caatacgcag cgcaagcgga
    17881 agatcgccgc agtcaatcgc ccacaccatc agcgtggcaa tcacttcgtc ctgttgcccg
    17941 ccgtcagcct ccagcgttcc ctcaatccag ccggaaaagt ccggcaacaa cgcttttttg
    18001 atgtcggctt tcgcgcttct ggcctgtacg cccttaagcc gggcctgtgc cagacgcaga
    18061 cgatacagca cctcttcatg cgcggtacgc gcggcgtggt ccacaccttc attcgcccgg
    18121 cctgcgcgct gtgccatcac gttctgccag tgttgctgtg caggagtaat cattttttct
    18181 ctccgttaca ggcgggcatg atgcccgccg tgagttgatt agctgtcggc gaacttcagg
    18241 ccagtgacca tcgcgcactt gccatagtct tcaacgacat aagcgtcatt gatggactgg
    18301 taggtggcga tgcggttgta ttccggttcg tctttcatca gacgacgcat t
//
LOCUS       DAZOYS010000073        18273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOYS010000073 DAZOYS010000000
VERSION     DAZOYS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.073"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..591
                     /locus_tag="ACHUN9_002174"
                     /pseudo
     CDS             1..591
                     /locus_tag="ACHUN9_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            694..1196
                     /locus_tag="ACHUN9_002175"
                     /pseudo
     CDS             694..1196
                     /locus_tag="ACHUN9_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1263..2720)
                     /gene="nuoN"
                     /locus_tag="ACHUN9_002176"
     CDS             complement(1263..2720)
                     /gene="nuoN"
                     /locus_tag="ACHUN9_002176"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0695567.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2727..4256)
                     /gene="nuoM"
                     /locus_tag="ACHUN9_002177"
     CDS             complement(2727..4256)
                     /gene="nuoM"
                     /locus_tag="ACHUN9_002177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0695568.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4420..6261)
                     /gene="nuoL"
                     /locus_tag="ACHUN9_002178"
     CDS             complement(4420..6261)
                     /gene="nuoL"
                     /locus_tag="ACHUN9_002178"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0695569.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6258..6560)
                     /gene="nuoK"
                     /locus_tag="ACHUN9_002179"
     CDS             complement(6258..6560)
                     /gene="nuoK"
                     /locus_tag="ACHUN9_002179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0695570.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6557..7111)
                     /gene="nuoJ"
                     /locus_tag="ACHUN9_002180"
     CDS             complement(6557..7111)
                     /gene="nuoJ"
                     /locus_tag="ACHUN9_002180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0695571.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7123..7665)
                     /gene="nuoI"
                     /locus_tag="ACHUN9_002181"
     CDS             complement(7123..7665)
                     /gene="nuoI"
                     /locus_tag="ACHUN9_002181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0695572.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7680..8657)
                     /gene="nuoH"
                     /locus_tag="ACHUN9_002182"
     CDS             complement(7680..8657)
                     /gene="nuoH"
                     /locus_tag="ACHUN9_002182"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0695573.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8654..11380)
                     /gene="nuoG"
                     /locus_tag="ACHUN9_002183"
     CDS             complement(8654..11380)
                     /gene="nuoG"
                     /locus_tag="ACHUN9_002183"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0695574.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11433..12770)
                     /gene="nuoF"
                     /locus_tag="ACHUN9_002184"
     CDS             complement(11433..12770)
                     /gene="nuoF"
                     /locus_tag="ACHUN9_002184"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0695575.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12767..13267)
                     /gene="nuoE"
                     /locus_tag="ACHUN9_002185"
     CDS             complement(12767..13267)
                     /gene="nuoE"
                     /locus_tag="ACHUN9_002185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0695576.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13270..15072)
                     /gene="nuoC"
                     /locus_tag="ACHUN9_002186"
     CDS             complement(13270..15072)
                     /gene="nuoC"
                     /locus_tag="ACHUN9_002186"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0695577.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15166..15828)
                     /gene="nuoB"
                     /locus_tag="ACHUN9_002187"
     CDS             complement(15166..15828)
                     /gene="nuoB"
                     /locus_tag="ACHUN9_002187"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0695578.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15844..16287)
                     /gene="nuoA"
                     /locus_tag="ACHUN9_002188"
     CDS             complement(15844..16287)
                     /gene="nuoA"
                     /locus_tag="ACHUN9_002188"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0695579.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16916..17854)
                     /gene="lrhA"
                     /locus_tag="ACHUN9_002189"
     CDS             complement(16916..17854)
                     /gene="lrhA"
                     /locus_tag="ACHUN9_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0695580.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4553 a         5089 c         4713 g         3918 t
ORIGIN      
        1 ctccctctga ttatttttac caaccctggg tcactgcatt atcgccaacg ccccgacacg
       61 tcagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg catccacgta
      121 atgccgacaa tattgataat ctgaataggt ccgtagagta tataaaagag ttctttgtca
      181 gtagcggtgc cagagttacc gcgcagagcg tccccgattg gctattacga ctctgcgcct
      241 ggtagccagg attaccctta cccgcaatgt cctggcttta tcccgatcgg ggggacttta
      301 ttgcctggtc ggcagaatac aggatatcaa cgccgttcgt caggtaaaaa cggcattgtt
      361 atcatcccgg gatttatctg tttattctat gaatgcccca tggtttattc ccggtattga
      421 tttctctgac cacctgaatt attggcaaca cgatattccc gccgtaatga ttactgacac
      481 cgctttttat cgtaataaac aataccactt gcccggtgat acagcagaca gattgaatta
      541 tcagaaaatg gctcagatgg tgttataacc ttgttacaca acagtaaata ataaaaattt
      601 ctcgaggcgg gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga
      661 tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa tgattggtta ttttgcagaa
      721 attgactcag aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat
      781 atacatgaca cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta
      841 cactttgggc tgacaggcac ccctgctttt gatcccgcga agaatgatcc gctgagtcgt
      901 gccgtgcttg gtgaacacag cctcgaagat ggcatagatg gcttcctggg attgacctgg
      961 aatcaggagt tagccgcgac aatcgaccga ctggaatcac tggatcgaag tgagctgcgt
     1021 aaacagtttt ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca
     1081 gaagagttag aggggcaact ctttgcttcg atcatgctga tatggaaaag ctgattagcg
     1141 cgtatcgacg tatgctgcga caagggaatc atgcgctgac ggttattgtt gggtgatggt
     1201 gtcagggcac caaaaaaaac cgccgaacat gtcggcggct ttctgactta caaactgata
     1261 gcttacatca gcggcattgc taaccgcaca atgctaatca gcggttgtgg ccatacaccc
     1321 agcaccagta ccaacagtgc agagatcagc accacgatac cgcctgcgct gtactgccag
     1381 tttgacggtg catcgcgacc cggttgttcc ggggcgtgca ggtacaggct caccgccacg
     1441 cgcaggtagt agtaaaggcc gcttgccgaa ccgacaacca cggcgcccac cagccaccac
     1501 aagtgtgcct ggacaccgac cgccagcacg tagaacttac caataaagcc cagcgtcatc
     1561 ggaataccgg ccagagacag catcatcacc gtcatcaccg ccgcgaggat cgggcgatgc
     1621 cagaacagtc cgcggtaaga gtacagggaa tcagcatccg ggccacgata cgggctggac
     1681 atcaggctga ccacgccgaa cgcgccgagg ctgctaaaca gataaccggc caggtaaacc
     1741 cctaccgctt ccatcgacat ctcgccagtt tgcagcgcaa tcagcgccac cagcagatag
     1801 ccgaggtgag agatagatga gtaaccgagc agacgtttga tattggtctg gctcagcgcc
     1861 atcaggttac cgaagatgat ggaggcaaag gcgataatcg ccagcaccac gcgaatcgct
     1921 tcgctgtcac ccatcggtgc gtagaggaac agacgcatca ccacaccgaa gatagcgatt
     1981 ttgctcgccg tcgccaggaa agtggaaacc ggcgcaggcg cgccctggta tacgtctggc
     2041 gtccacaggt ggaacggcac cagagagagt ttgaagccga ggccaacaat catcaggccg
     2101 aaacctgcca gcaacagcgg ctcgttgagc ataccgtcgc caaggttttt acccaacgcg
     2161 acaaacgaca ggtcgccaga ctgcgcatac accagcgcca taccaaacag caggaaagaa
     2221 gacgctgcgg cagaaaggat ggtgtatttg atactggctt ccagtgaacg tttctggcgg
     2281 aaagcgtaac cgaccaggcc aaacagcggc aaagagatca gttcgatacc gaggaacaga
     2341 gacgccagat ggttggcatt cgccagcagg atcccgccca gcgcggcaat taacaccaac
     2401 aggtagaact cgtccttgtt gtcgttatag ccttcaagcc acgggtaggc gaaagtacag
     2461 gtggcgaggc tcgccaacaa taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc
     2521 atcagcggcg taacgtccat agcgcctgcc tggccgacaa accagagcga aaccagcgcc
     2581 gcgttaagcc caataaccga gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg
     2641 gagagcatca caaccaccac cgtcaagccg acgatcagca acggtagcag tgcgatcagg
     2701 ttttgtggag ttattgtcat ggcgatttac ggccttgtag tagtaacgga attaacaaac
     2761 cactgctgga tattgccaat cgcggagtgc gaggtatcca gaatcggctg cggatagaag
     2821 ccctgcagta ccagcagcac caccagcaac aggatcataa acagctcacg cagcgacatc
     2881 cctggcagtt cctggctggc aatctcgctt ttcgctttac cgaagtaagc gcgatgcaac
     2941 atcgccagcg aataaacaga tgcaaagacc agcccaaagg tagagataac ggtaatcacc
     3001 gggacaacct ggaagctgcc gaacagaatc ataaattcgc cgacgaagtt accggtgcca
     3061 ggcatcccaa gcgttgccac cgcaaagaac agcgacagtg ctggcagcca tttcatcttg
     3121 ctccacagac cgcccatcat gcgcatgtcg cgggtatgga tacgttcata aagctgacca
     3181 caaagaataa acagacccgc cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc
     3241 tggtaggcca actggctgcc ggtgtagata gcaatcagca cgaagcccat gtgggaaacc
     3301 gaggtgtagg cgatcagacg tttgatatcg gtctgggcga aggccatcca cgcaccgtag
     3361 aagatgccga taacacccag ccacatagca attggcgcga actctgccga cgcgttcggg
     3421 aacagcggca gggagaaacg cagcaaaccg taagcggcag ttttcagcaa gatccccgcg
     3481 aggtcaacgg aaccggcggt cggagcctgg gagtgcgcat ccggcagcca gccatgcagc
     3541 ggaaccaccg gcattttgac tgcgaaggcg atgaagaagc ccagcattaa caggtattcc
     3601 gcaccactgg acattggcgt attcagcagc tcttcatagt tgaaggtcca gacgccggtc
     3661 gcattgtagt gaacaaaaac cagcgccagg atggcaatca acatcaccag accactcgcc
     3721 tgggtgtaaa tgaagaactt ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta
     3781 tgcccccaca gtgcgatcag gaagtacatc ggcaccagca tcatttccca gaagaagaag
     3841 aacaggaaca tgtcgatggc aaggaacacg ccgataacgc cgcccaggat ccacatcagg
     3901 ttgaggtgga agaagccctg atatttttcg atctctttcc acgaacatag taccgccagc
     3961 acaccgagca gaccggtcag cacgaccatc agcagcgaca gcccgtcaat ggcgagatga
     4021 atagagatac ccaaacgcgg gatccacggc atgtcgaatt cagactgcca ctgcggaatt
     4081 ccggcggatt gcgtcagtga ataaccgccc tgcaaccaca gttgcagcga cagcgccagc
     4141 gtcaatccca tggtgatcag cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc
     4201 tgccagcaca ggaagccgcc aataaaggga attaatatta gccagggtag taacatggcg
     4261 atctttattc cttgtaaaag tcccgtcagg accggatttt caacgaattc tcacgacaga
     4321 aaattctctt cggactgggg gtagtgtcgg atgcaccgcg aaggcggctt atccgaccta
     4381 cgttcttata tgcccagggg caatcccaca atccttaact caacgcagta ccatcaacag
     4441 tgccagcacc acgaccgcac cgatgctcat ggatgccaca taccagcgca gatagccgtt
     4501 ctcacttaac agcagacctt tacctgcaaa gcgggaaagg acagccggaa tgttcatcat
     4561 tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc aggaacggcc tgacgaacac
     4621 tttgtcgtac agccagtcaa agccccaggc gttgtaccac caggtactca gcaggcggcc
     4681 cggcgcactg ttggcgatgg aggtcaccag agtacgttta cccagccaca gccaagctgc
     4741 cagcagaatg ccgacaatcg cgaccacgcc agaggtaatt tccagggtca acatgctgcc
     4801 gtgcgccagt tccgtcgttt gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc
     4861 aacgaaggtg gaaaggatca gcagcacaat cagcggcagg ctgtgagtta cccctttcac
     4921 ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg aaaatcatac ggaaggtgta
     4981 gagcgaggtc ataaacgcac cgaccagacc tgccaccatc agattgatat gaccattcgc
     5041 catcgcaccc gcgaggatct catccttact gaagaagccc gcagtgacca gcggcagtgc
     5101 cgacagtgct gcgccgccca ccaggaagca gagataaacc agcggaatag atttacgcag
     5161 accgcccatc ttgaagatgt tctgttcgtg atggcaggcc agaatgacgg aaccggatgc
     5221 caggaacagc agcgctttaa agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca
     5281 tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg ctcatggtag agtaagcgag
     5341 aacacgtttg atgtcggtct gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc
     5401 cccgacaatg cccaccagat gcagaatttc cggcgtcatc aggaacaggc cgtgggtacg
     5461 ggcgatcagg tagacacccg cggttaccat ggttgcggcg tggatcagcg cggagacagg
     5521 cgtcgggccc gccatcgcgt cggcaagcca tgtctgcaac ggcaactgcg cagatttacc
     5581 gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa
     5641 gtgcgctggt gccagttcca ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag
     5701 aatgaacagt gcgaaagcga ggaaaacgtc acccacacgg gtcacgacga acgctttcat
     5761 tgccgctgcg ccattcttcg gatcggtgta atagaaaccg atcagcagat aggagcacag
     5821 gcccacgcct tcccagccga ggtacatcag cagcaggttg tcggcaagca ccagaactac
     5881 catgctggcg atgaacaggt tggtgtaggc gaagaagcga gagtagccct cttcaccgcg
     5941 catgtaccag gaggcgtaca tgtgaataag gaaacccaca ccagtgacca ccgagagcat
     6001 ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat
     6061 ccacgtccac agcggctggc tgtatgcctg ctcgccgtta gcgaagaaat caacgccgat
     6121 aaaggcggtt accagcgccg ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt
     6181 ttcagaccag cgcccacggg agaacgccag caggacgaag ccaatcaatg gcaaaataat
     6241 ggttaaggca agcatgttca tccgcgcatc tcacttactg aatcgatgtt caggttctgg
     6301 cgacgacggt gaagttgcag cagcagcgca aggccgatac tcgcttctgc cgccgcgagg
     6361 ctgatggcga gaatgtacat cacctgaccg tcggtctgac cccagtagct tccggcgaca
     6421 acaaaggcca gcgcggaggc gttaatcatg atttccagac caatcagcat aaacagcaga
     6481 ttgcgacgga taaccagacc ggttaagcca agaatgaata agattgccgc gaggatcagt
     6541 ccatgttgta aggggatcat gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac
     6601 gattgctcag cacttcaccc gcacgctctt cacgaccgac gtggaaggcc acaaccagac
     6661 ctgcgagcag cagcatagaa gccagttcca ccgccagtac gtaaggcccg aacagcgtaa
     6721 tacccactgc tttagcactg attggcgtac cgtcgatacc ctgatcgtta acaccgagga
     6781 tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat tgccggacca atccacacct
     6841 gcggtttcag ccactggcgt tcctgttcga tttctgaacc gcccaggttg agcatcatca
     6901 ccacgaacac gaacagcacc ataatggcac ccgcgtagac gataatttcc agcgcaccgg
     6961 cgaagtaagc gcccagtgag aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt
     7021 acagcagtgc gtgtaccgga ttggtatggg tgatcactcg caaggtcgca agtatggcta
     7081 tcaggccaca gatataaaaa gcgaactcca ttgcccctct ccttacggta acaggctctt
     7141 gacgtcgata ggcttggctt cgttctctgc ttcgccctta tctttgccgt cgattgccat
     7201 acctgccatc cggtagaagt tatattccgg gtatttgccc ggaccggaga tcagcagatc
     7261 ctctttttcg taaaccagat cctggcgctt gtattccccc atttcgaaat ccggggttaa
     7321 ctgaatcgcc gtggtcggac aggcttcttc gcacagacca cagaaaatgc agcgtgagaa
     7381 gttgatgcgg aaaaattccg gataccagcg accgtctttg gtttctgctt tttgcagcga
     7441 gatacagccg accgggcagg ctaccgcgca gaggttacag gctacgcaac gctcttcgcc
     7501 gtccgggtcg cgggtcagaa cgatacgacc acgataacgg ggcggcagat agatcggctc
     7561 ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc aggccgatca tccagatact
     7621 acgaacctgg gtgccgaaac ctactaacaa ttcttttaag gtcatggtct tattgcccct
     7681 tattgcgcct gccagagaat gacagccgcc gttaccagca agttgatcag cgtcagcggc
     7741 aggcagattt tccagccgaa ggacattacc tggtcataac gcggacgcgg taacgacgca
     7801 cgaatcaaaa tgaacatcat cataaagaac gcggttttca gcgcgaacca gatgaatggc
     7861 ggtaacaacg ggccttgcca gccaccgaag aacagcgtca ccatcaatgc agagatggtc
     7921 acaatcccga tgtactcacc cacgaagaac agaccgaact tcataccgga atattcaatg
     7981 tggtaaccat ccgccagttc ctgctcggct tccggctggt caaacgggtg acggtgacat
     8041 accgccacgc ccgcgatggc aaaggtaata aaaccaaaga attgcgggat aacgttccac
     8101 acatgcgcct ggctgttgac gatgtcggtc atgttgaatg aaccggcctg cgccaccacg
     8161 cccatcaggg aaagcccgag gaacacttcg tagctcaggg tctgcgcaga agcacgcatc
     8221 gcacccagca acgagtattt gttgttactt gaccagcccg caaacagcac cgcgtaaacc
     8281 gccagacctg ccatcatcag gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa
     8341 cccggactga ctggcacaat cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt
     8401 gccagggtaa agatgacgcg atccgagaat ttcgggatcc agtcttcttt aaagaacatt
     8461 ttgatcatgt ccgcaaccag ctggagcgaa ccgccccagc caacacggtt aggtccgtaa
     8521 cggttctgga acagacccag caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg
     8581 acaaccacca gcaggatcac caccgctttg aggatggtca gcaggatctc aatcagttcc
     8641 ggtgatatcc aactcattgt tgtgcctcct tgagatcctc aagatgcgcg ccagccagca
     8701 ccggagcaat accggacata cccatcggca agcccacctg ccctgccgtc agcccttcag
     8761 cgatttcaac cggcagcgtg accgtattgc catcgtaact aaaggagacg tgtgtacctg
     8821 cgttcacacc taacttcgcg gcatccgctg ggttgagttt gatgtacggc tgcggcatac
     8881 ggctctggaa gaccggagca cgctgtgaca attcatcgct gccaaacagg tggtaatacg
     8941 gcgcgatacg ccatttcccg tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat
     9001 ccagaccatt ttcgctggtt tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc
     9061 cgcccacttc gtcctggaat ttgttccacg cctgcgggga gttccagccc ggcgcccagg
     9121 caaacggcac ttgcgaacgg tgcgcagtcg gctggttgtt accttccatc gagaaggtga
     9181 acatggtgtc aatatcctgc ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg
     9241 cggtacgacc gctgtaacgg tgcggttcac gggccagttt ctgcccacga atacggaatg
     9301 tcgcatccgg cgcagcatct ttgatacctg ccagttccgg gattttcgcc acaacagcgt
     9361 caatcacatg gtcgagctgc gtccagtcca cttcacggct cagcagggtg ctgtgcagcg
     9421 agtgtaacca gcgccagctt tccagcatga cagttttgct gtcgtaataa gcaggatcgt
     9481 aaacctggaa gaaacgttgg gcgcggcctt cgttgttgat caccgtaccg tcgctttcag
     9541 caaaactggc agcagaaagt accagatggg cgttttccat aatcgctgtg cgttgatgat
     9601 caaccaccat caccagcggc gctttagcca gcgcagcatt cacgcgggta gcggaagcgt
     9661 gacgatgcag atcgttttcc agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg
     9721 tcaacgcttc ttcaagcgaa ccgccaccca taatgcccag ccccatgctg ttgacggaac
     9781 gggcaatcat ggtgatacca acgtcagcgc cgcgaccttt cagggctttc gcgacgttag
     9841 ccgcagcctg aatcacctct atgctaccgg cgttcgtccc ggagataatc aacggtttct
     9901 tcgcacctgc cagtgcctgc acgatgacgt cgattttgct ttgcagctca ggttcgatac
     9961 cgtcaaccgc tggcgcagag ttatccagcg catgggcgat ggcaaaacct aaacgcgcct
    10021 gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc atccagacgg gtgtcatcaa
    10081 cgttggtaac aaacagcgga tgcttcgcac gttgaccgat gttgaggatt gccgcaatct
    10141 gccagtcagc cactttctgt gctgccgcca tttcgcgcgc tttacctttc acagcctgac
    10201 gcactgccag cgcgacgcgc gcgccggtct gggtaacgtc ttcgcccagc accagtaccg
    10261 catcgtaaga ttcgatttcg cgcagagccg gagtataaat gccgccttca cgcagcactt
    10321 tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat accggtgtag aagttttctt
    10381 cgcccaccag ttcacgcagc gcaaagttgc tttccacgct ggcacgcgga gaaccaatac
    10441 cgatcacttt cttcgactga cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt
    10501 tgagggtaat gaaatcatcg ccacgacgct gtactggctg acgcggacga tccttcaggt
    10561 tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa gtagtggttt accgtaccgt
    10621 tgtaacggtt ttcgatacga cgcagttcgc cgtaacgttc accggggctg atgttacagc
    10681 cgatggaaca ttgctggcag atgctcggcg caaactgcat atcccattta cggttgtaac
    10741 gctcggagtg cgttttgtcg gtaaatacgc cggtcgggca aatttcgacc aggttaccgg
    10801 aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac
    10861 cgtaaacgcc cagatctgta ccgtcagcgt aatctttgta gtaacgcaca cagcggtagc
    10921 aggcgatgca gcggttcatt tcgtgagaga tgaatggccc caaatcctga ttacggtggg
    10981 tacgtttggt gaaacggtag cgacggaagc tgtgtccggt catcacagtc atatcctgaa
    11041 gatggcagtt accgccttct tcacataccg gacagtcgtg cgggtggttg gtcattaacc
    11101 actcgaccac actttcacgg aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc
    11161 catcggaagc cggtgtcata caggacatca ccaggcgacc acgcgtgtct tccgcgtttt
    11221 ggtattgctt caccgcacac tggcggcaag caccgacgct tcccagcgcc ggatgccagc
    11281 aaaagtaagg aatatcaagg cccagagaca gacaagcttc cagcaggttg tccgctccgt
    11341 tgacctcgta ttctttgccg tctacatgaa ttgtagccat tagcatgctt ccagttttct
    11401 cggtcaggga ccgagcgtta atcgaaattc ggttaccagc gctctttcag caggttcggc
    11461 tgaatcccat taatcaaatg ggtattgctg aacggctgtt tgattcccgc ctcaaattct
    11521 tcgcggaaat atttgatggc gctctgtaac ggctccactg caccaggtgc gtgggcacag
    11581 aaagttttac ccgggcctaa gaatcgacac agttgctcaa gtgtttcgat atcgcccggc
    11641 tggccttcgc cacgctccag cgcacgcaga attttcacgc tccacggcag accgtcgcgg
    11701 cacggcgtac accagccgca ggactcacgg gcgaaaaact cttccaggtt acgcaccagc
    11761 gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg ccgtacccag acggctgccc
    11821 gctttaccga tactttcgaa ttccatcggc agatcaaggt gcgcttcggt caggaagtca
    11881 gtccccgcgc cgcctggctg ccaggcttta aatttcagac catcacgcat accaccggcg
    11941 taatcttcga ggatctcgcg tgcggtggtg ccgaacggca gttcccacag tcccggattt
    12001 ttcacccgac cggagaagcc catcagcttg gtgccagcat ctttactttt cgagatgttc
    12061 tgataccact ccacgccgtt agcgaggatc gccggaacgt tacacagggt ttcgacgttg
    12121 ttgacacagg tcggtttgcc ccatacgccg gaggttgccg ggaagggggg tttcgagcgt
    12181 gggttagcac gacgcccttc cagggagttg attaacgctg tttcttcccc gcagatgtag
    12241 cgccctgccc cggtatggac gaacagttcg aagtcgaaac ctgttcccat aatgtttttg
    12301 ccaagcaagc ccgcttcagt ggcttcggca atggcgcggc gcagatttac tgccgcttcg
    12361 atatattcgc cacgcaggaa gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg
    12421 agcatacctt ccaccagcag gtgcggcagt tgctccatca acaggcggtc tttataggtg
    12481 cccggctcca tttcatcggc gttacacagc aggtaacgga tgttcatgga ttcgtctttc
    12541 ggcatcaggc tccacttcag gccagtcgaa aagcccgctc cgccgcgccc tttcagacca
    12601 gcgtctttta cctgattaac gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc
    12661 ccttcgtaac cgtttttgct gcggtattcg tccagccaca ctggctgttt gtcatcgcgc
    12721 agacgccagg tcagcggatg cgtttcggga gtacggataa tgtttttcat ttataccgct
    12781 ccagcagttc agggatcgct tccggggtca gatgcgcgtg agtgtcctca tcgatcatca
    12841 tgtttggccc tttatcacag ttccccaggc agcaagttgg cagcagcgta aagcggccat
    12901 caaatgtcgt ttgccctggt ttgatgttca gctttttctc gagcgccgcc tgaataccct
    12961 gatacccgtt gatatgacag accacgctgt cgcaataacg gatcacatgg cgaccaaccg
    13021 gctggcggaa gatctgactg tagaacgttg ccacaccttc gacgtcgctt gccggaatac
    13081 ccagcacatc ggcgatcgcg tggatcgcac catccggcac ccagccacgc tgcttctgaa
    13141 cgattttcag cgcttcaatg gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct
    13201 caatcgcttc acgctctgcc gcactcagct caaaagcctc ggtttgtggt tgttgattct
    13261 cgtgcataat tagcggtcca catctgacat aacaaaatcg atactgccca gataaacaat
    13321 caggtcagac accaggctgc cgcggatcgc cgccggaatt tgctgcaaat gcgcataact
    13381 cggcgtgcgg atacgagtgc gataactcat ggtgctaccg tcgctggtca ggtagtaact
    13441 gttgatcccc ttggtcgcct caatcatctg gaaagattca ttggcaggca tcaccggccc
    13501 ccacgacact tgcaggaagt gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt
    13561 cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg ccttccggca tgttgttgag
    13621 gcactgctca agaatgcgca gactctggcg cagctcttcc actttcagca ttacgcgggt
    13681 gtagcagtca gaaacaccac caccaaccgg gatttcaaag tcgaagtttt catagccaga
    13741 ataaggacgc gccttacgca cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt
    13801 ggtgccccac tccagcgcct ctttcgcgcc ataggcggca acgccctggg aacgaccttt
    13861 cagaatggta ttttgcagcg ccgctttctc gtaagacgcc agacgtttcg gcatccagtc
    13921 gaggaactca cgcagcaggc gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat
    13981 acggaaccac gccgggtgca tacggaaacc cgtgatcgct tccaccagat cgtaaatttt
    14041 ctgacgatcg gtaaaggcga agaacacggg cgtcattgcg ccgacgtcct gaataaaggt
    14101 cgagatgtac agcaggtggc tgttgatacg gaacagttcg gagagcataa cgcgaatgac
    14161 gttaacgcga tccggcacgg tgatcccggc cagtttctct accgccagca cgtaaggcat
    14221 ttcgttaacg cagccgccga ggtattcaat acggtcggta tacggaatgt agctgtgcca
    14281 ggactggcgc tcgcccattt tctccgcacc acggtggtgg tagccgatgt ctggtacgca
    14341 gtcgacaatc tcttcgccgt cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg
    14401 gtggttcgga ccgaggttga ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc
    14461 ccactcttcc ggtttgaagg tcagggcttc catctccaga tcctgtttgg ctttggtcag
    14521 ctcaaacggc gagaattcgg tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt
    14581 ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa
    14641 cagatcccag gtttcacgct cataccagtt agcgttcggg aacagtttgg tgaaggtcgg
    14701 tacgtgcagg tcgttttctg ccagcgccac cttcagcatg atgtcgcggt tacgatcgat
    14761 agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg
    14821 cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt
    14881 taagaaatcg ccaacttcca gtaattgttc acgcttgatc cacacaacgg gaaccccggt
    14941 gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac
    15001 cggatcatca agatgatcac gggtctgcca ggcgggttct tgcgcggtta agtcggtcat
    15061 attgttcacc attgcaaatg gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag
    15121 atttaggaat aagcgaagaa aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg
    15181 tgtacgcagg ttggttacgg caatgcgttc accgcgcttg cgttcgcgct ctgattgcat
    15241 attggcgcga taaacgccct gatcgccaac cacccaggag agcggacgac gttctttgcc
    15301 gatagattcc tgcaacagca tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc
    15361 cgggatatac acatcaaccg ggatgaattt atcgacgccc tgcacaacgg aataaatatc
    15421 gtacatacca ccagagttgg cacaggcacc cattgagata acccattttg gttccagcat
    15481 ctggtcatac agacgctgaa taaccggtgc cattttggta aagcaggttc ctgcaaccac
    15541 catcaggtca gcctgacgcg gcgaagcacg caatacttct gcgccaaaac gcgccacgtc
    15601 atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag caggaaagac cgaagttata
    15661 cggccaaatt gagtttttac gaccccagtt aaccatgtca ttgagcttgc ccataaacac
    15721 gtttttgtta acttcttgct ccagagggtc ggttacgatc tcctgctttt gcagggggta
    15781 acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta taatccatct taatgcctcg
    15841 cggttagcgt tgacgattag cgatactgtt cgtttccggg ttcatacgct cgcggcgtga
    15901 acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga taaaccagac ctgccagtaa
    15961 cacaaaaata aaaattgcag cttccacaaa gcctacccag ccgctttcgc ggatagaggt
    16021 tgaccatgcg aacagataca gcgcttcaac gtcgaagata acgaagaaca tggccaccag
    16081 ataaaacttg gcggacaggc gtaagcgggc ggagccgacc gagtcgatac cggattcaaa
    16141 cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat
    16201 caggcaacac aggccaatgg caacgataag aaagatagcg aatgcccagt gatgagcgat
    16261 gacttcagtg gatgttgaca tactcattgc ttactcatca aaagtagcgc cagattcact
    16321 gctcttcacg gcagatggac gccacatcga ttcatgggga ggaataaaaa aaaccttaca
    16381 atcactgtag aaattctttt atacagctaa ttgatgtggt cttttactcc tttctataac
    16441 cttttgtcaa ctttaacaaa agtttcttca cattagttta cataatatca acaccattag
    16501 catttaatgc cctttcacgc cagatccttg acgactccag gataattaga tgttgttgaa
    16561 tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat tttgacagga atttgtgtcg
    16621 attcctcccc ccaaaagaga gtattttctt gatctgtgac acgcttttgt cattccataa
    16681 caaaaacgca gcaacaaatt tacgtatttt taacatcatt gtagcaggtg atttttttca
    16741 ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga acagttttgt
    16801 acgttctgca ctatgcgagg aaggattttt actaaaaaaa agccgctggg gtttaaaaca
    16861 cccccagcgg ctcgttttta cactattgtc tcaggaatta tctatcgtcc gtcgattact
    16921 cgatatccct ttcaatcaac aatgaatcat ccccttccgg agcagacatt gggctgtatt
    16981 gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc tcattattac
    17041 tggacgggtc atagcacagc aaatattcgg tatcaggtag cggcggtaag ccatctactc
    17101 cactcagcac acgcaggtcc gggctcatca tttcaatcgg ccttgccgtc acgccaagac
    17161 cggctttcac tgccgcacga acggccggaa gcgtcgaggc gacataagcc agtcgccatg
    17221 gaatatctgc tttattaagc gtcgccagca ccatatcgcg aaacggactt ggatcatcca
    17281 gcaatacaag agggatcggc tcgccttttt gcaatacgta ttccgctgcg cagtaccagt
    17341 gtgttggcga ggtacgcagg ttcaatgcct taaacgctga aggacgatgg gtggttacca
    17401 tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt ttaacgcgga
    17461 catccagcgc taatttcgga taaaccgaac tcacgcgatt taacaggaaa ggtaagatcg
    17521 tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta ctgaacatta
    17581 atgatgagca ggcctcatca ttaaaacgca gtattttcct ggcgtaacca agaagttgaa
    17641 tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct ttcccaacga
    17701 gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac acagcggcag
    17761 ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc agatcgaggt
    17821 cgaggttaat tatcggacga tttgcactta tcatatatta tcacttactg gcggctcata
    17881 ctgagctggt taatgctgtg cacacacaaa caagcaattc catttgtaat gtgcctccct
    17941 ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat atgagttgca ctaaaaaagc
    18001 gactcacatt gttccgttat aatgcctgaa gtagatcaca gaatatatct tcagggatcg
    18061 catatctatt aagttactca ctcttttcta tttatgacat gcgcgtgttt gtataaatgt
    18121 aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga acacgtacaa
    18181 acagcaatgg tgaggcggca tcaagagcag ggatccgtca tttatgcgag cattttaccc
    18241 caaaaacctt ttatttataa gggtcattgc gaa
//
LOCUS       DAZOYS010000074        18125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOYS010000074 DAZOYS010000000
VERSION     DAZOYS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.074"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            151..576
                     /gene="nudI"
                     /locus_tag="ACHUN9_002190"
     CDS             151..576
                     /gene="nudI"
                     /locus_tag="ACHUN9_002190"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0695581.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(615..1217)
                     /gene="ais"
                     /locus_tag="ACHUN9_002191"
     CDS             complement(615..1217)
                     /gene="ais"
                     /locus_tag="ACHUN9_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0695582.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1525..2664
                     /gene="arnB"
                     /locus_tag="ACHUN9_002192"
     CDS             1525..2664
                     /gene="arnB"
                     /locus_tag="ACHUN9_002192"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0695583.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2668..3636
                     /gene="arnC"
                     /locus_tag="ACHUN9_002193"
     CDS             2668..3636
                     /gene="arnC"
                     /locus_tag="ACHUN9_002193"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0695584.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3636..5618
                     /gene="arnA"
                     /locus_tag="ACHUN9_002194"
     CDS             3636..5618
                     /gene="arnA"
                     /locus_tag="ACHUN9_002194"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0695585.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5615..6505
                     /gene="arnD"
                     /locus_tag="ACHUN9_002195"
     CDS             5615..6505
                     /gene="arnD"
                     /locus_tag="ACHUN9_002195"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0695586.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6505..8157
                     /gene="arnT"
                     /locus_tag="ACHUN9_002196"
     CDS             6505..8157
                     /gene="arnT"
                     /locus_tag="ACHUN9_002196"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0695587.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8154..8489
                     /gene="arnE"
                     /locus_tag="ACHUN9_002197"
     CDS             8154..8489
                     /gene="arnE"
                     /locus_tag="ACHUN9_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0695588.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQVTCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8489..8875
                     /gene="arnF"
                     /locus_tag="ACHUN9_002198"
     CDS             8489..8875
                     /gene="arnF"
                     /locus_tag="ACHUN9_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0695589.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8869..9135)
                     /gene="pmrD"
                     /locus_tag="ACHUN9_002199"
     CDS             complement(8869..9135)
                     /gene="pmrD"
                     /locus_tag="ACHUN9_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0695590.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(9245..10600)
                     /gene="menE"
                     /locus_tag="ACHUN9_002200"
     CDS             complement(9245..10600)
                     /gene="menE"
                     /locus_tag="ACHUN9_002200"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0695591.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(10597..11559)
                     /gene="menC"
                     /locus_tag="ACHUN9_002201"
     CDS             complement(10597..11559)
                     /gene="menC"
                     /locus_tag="ACHUN9_002201"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0695592.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(11559..12416)
                     /gene="menB"
                     /locus_tag="ACHUN9_002202"
     CDS             complement(11559..12416)
                     /gene="menB"
                     /locus_tag="ACHUN9_002202"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0695593.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12431..13189)
                     /gene="menH"
                     /locus_tag="ACHUN9_002203"
     CDS             complement(12431..13189)
                     /gene="menH"
                     /locus_tag="ACHUN9_002203"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0695594.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13186..14856)
                     /gene="menD"
                     /locus_tag="ACHUN9_002204"
     CDS             complement(13186..14856)
                     /gene="menD"
                     /locus_tag="ACHUN9_002204"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0695595.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14945..16240)
                     /gene="menF"
                     /locus_tag="ACHUN9_002205"
     CDS             complement(14945..16240)
                     /gene="menF"
                     /locus_tag="ACHUN9_002205"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0695596.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16319..16624)
                     /gene="elaB"
                     /locus_tag="ACHUN9_002206"
     CDS             complement(16319..16624)
                     /gene="elaB"
                     /locus_tag="ACHUN9_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0695597.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16679..17140)
                     /locus_tag="ACHUN9_002207"
     CDS             complement(16679..17140)
                     /locus_tag="ACHUN9_002207"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0695598.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17205..18122
                     /gene="rbn"
                     /locus_tag="ACHUN9_002208"
     CDS             17205..18122
                     /gene="rbn"
                     /locus_tag="ACHUN9_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0695599.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         4291 a         4839 c         4587 g         4408 t
ORIGIN      
        1 ttaaacgccg aaaaaataaa cacactttac cgatacacac aaaaataata ataagtgcag
       61 ttatcatcgc aaaagagatt actgtcactt tcacatattt ctgagttacc gtattctcat
      121 ggtttctttt tatttataag gaatacccct gtgcgacaac ggactattgt atgccctttg
      181 attcaaaatg atggtgctta tttgctgtgc aaaatggccg acgatcgcgg cgttttcccc
      241 ggtcaatggg cgctttcggg tggcggcgtg gagtctggcg aacgaattga agaggcacta
      301 cgccgcgaaa ttcgcgaaga actgggagaa cagctgcttt tgacagaaat cacgccgtgg
      361 accttcagcg atgatattcg caccaagacg tatgcagatg gtcgcaagga agagatttat
      421 atgatttacc tgatttttga ctgcgtttct gccaaccgag aagtgaaaat aaacgaagag
      481 tttcaggact acgcgtgggt aaaacctgaa gatctggtgc attatgattt aaatgttgcc
      541 acccgaaaaa cgttacgttt gaaaggtctt ctttaacaat gatgacagcc atcactggct
      601 gtcagattga aaatttagtg gttaacgaat tccccatcca gataaacttt gcctttttcc
      661 acatgcatga ctaaaccatc cagataatca ggtttaaatg tcgcatcacg cttattttta
      721 gcaatatatg tcaggcaatg attatgggtg aaaataacga tatttttatc aggcgctttg
      781 ctttgtaagt ccttaattgc actataaatc tcattaccgc actgaagaag tcgtttatct
      841 accgtcagtt ttttacccgc ggaaaaccag gtcgccgact gaatggtccg gacggtatta
      901 ctggaataaa gatcgaaatc agggatgtca gcgctaaaag cgttgcccag ttcacgggca
      961 tcctgggtac ctttaaccgt aatacctgtt ttatctgaca agcattgatt ggttgaacga
     1021 tcgcaacgtt cagcatggcg aaacaaaacg acaaccgggt gctgctgtgc cagtctggcc
     1081 aatgttttat tgtcgatacg tggcaaacca ttgctactcc aggcggcatg agtacccagt
     1141 ccggcaattg cagcgagcgc cagtaaaatg atgaaatatt tttttgactt caatgatgag
     1201 cggcaaaaag ctaacacgta aactccacct atagacaagc gcaaccagac aattaccgtg
     1261 aaattgagcg acatttctgg cgataattag cagttggtgt aatattaaaa atcctacgat
     1321 gtcggcaaaa tgcctcaaaa ttttgccaaa tgcaaagcct aaataagaaa aaatataaaa
     1381 atttcaatat ttacgtctaa tattagtttc ttaaggttaa gttaatattc tatccttaaa
     1441 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
     1501 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
     1561 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
     1621 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
     1681 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
     1741 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
     1801 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
     1861 gcgatcgagt cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
     1921 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
     1981 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
     2041 accgctattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
     2101 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
     2161 gtcgatgcct atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg
     2221 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa
     2281 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
     2341 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
     2401 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
     2461 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
     2521 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     2581 tgttctttgc cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc
     2641 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     2701 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     2761 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     2821 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     2881 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     2941 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     3001 cgcctggtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     3061 caggacagct ggtttcgtaa aacagcttcg aagatgatta acaggcttat tcagcgcacc
     3121 accggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     3181 gatgcgatgt tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc
     3241 gcccgccgtg ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     3301 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     3361 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     3421 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     3481 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattgctc
     3541 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     3601 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     3661 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     3721 tttttaccca taccgataat cccgctgaaa aagcctttta tggttcggtg gctcgtctgg
     3781 cggcggaaag aggcattcca gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     3841 aacgcattgc ccaactctca ccagaggtga ttttctcttt ttattatcgc catcttattt
     3901 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     3961 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     4021 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     4081 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
     4141 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaattg
     4201 cccagcgcga aaacgaagcc acctgttttg gtcacagaac gccagatgac agtttccttg
     4261 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     4321 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     4381 ctcatgccag caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct
     4441 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     4501 gctcgcaatt agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     4561 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     4621 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     4681 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata
     4741 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc
     4801 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     4861 attttgaaga gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct
     4921 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     4981 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat
     5041 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     5101 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct
     5161 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg
     5221 atggcggaaa acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc
     5281 gcattatcga aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg
     5341 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     5401 cgctgcgcca tcacttccca ccgtttgcgg gctttcgtgt tgtcgaaagt agcagctact
     5461 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct
     5521 gcctggactg ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     5581 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga
     5641 tgtcgatacc tttcgtggca cccgtgaagg cgtgccacgt ctgctggaaa tcttgagtaa
     5701 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     5761 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     5821 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     5881 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     5941 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     6001 tgcacgcggt cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     6061 tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     6121 caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg
     6181 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     6241 agcagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     6301 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     6361 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     6421 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     6481 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     6541 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa
     6601 acgcgttatg cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat
     6661 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     6721 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     6781 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     6841 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     6901 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     6961 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     7021 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     7081 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     7141 atcagttgcg tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac
     7201 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     7261 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     7321 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     7381 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     7441 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     7501 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     7561 ggcttcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     7621 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     7681 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt
     7741 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     7801 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     7861 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     7921 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     7981 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     8041 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     8101 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     8161 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     8221 taacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     8281 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     8341 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     8401 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggcg
     8461 gcattgtgat cctcgggagt acggtgtaat gggcctgatg tggggattat tcagcgtcat
     8521 tattgcctca gttgcgcaat taagcctggg ttttgcggcg agtcatctgc cgccgatgac
     8581 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     8641 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact
     8701 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     8761 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     8821 tattatgagc gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt
     8881 ccctgccact ttacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     8941 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     9001 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     9061 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     9121 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     9181 aacagataaa cctattatca acggcaatgg aatggcaaaa ttgcgaagtg aaactaatga
     9241 tttcttaatc ctggcgttgc acccactctt ttaacgcctg acgtgaaatt ttaataccgc
     9301 cgtttttcag ctccggcggt aaagttagcc agcgcaccgg ttgttgaaaa cgagccagct
     9361 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     9421 gtcgatgacc aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcaggat
     9481 gtgcagcaat tacgcgctcg acttcttccg gctgaatacc ctctccgcca ctgaagaaca
     9541 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     9601 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     9661 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     9721 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa
     9781 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     9841 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     9901 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     9961 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
    10021 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
    10081 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
    10141 ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg
    10201 tcggctgcca cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaatg
    10261 ccggaaacgt gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca
    10321 attcttcaag caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
    10381 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
    10441 cgctgccctc aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
    10501 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
    10561 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaactgct ccagtacatc
    10621 agcgtccact aacggcagtg ggctccccgg ccagcgacga acctgctgcg cctgcatcag
    10681 atccagcgtg tccagtcccg gaatggtgtc tggcgttaac caggcagcaa tccgcgccag
    10741 ttgcgttaga cctaagctcg actcaatgga agaactgatc accgccgtca gccccagcgc
    10801 atgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat
    10861 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc
    10921 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
    10981 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
    11041 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
    11101 aattgcctcc agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac
    11161 tttgaccttc gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg
    11221 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
    11281 ctccgccaat gcacagctta cgccaaaggc aacggaaggc atctgcggta gctcgccatc
    11341 gcctgccagc cagttattta cccaagcaag cagcacaatt tgcgcctctt cccaggtttc
    11401 ctgactgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cttcacgcag
    11461 gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca tccccgcgtc
    11521 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    11581 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    11641 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gacccgcctg accgtcacag
    11701 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg
    11761 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    11821 cccatatcca gcgcctgttt tgcatcgtac tgacggcaca ggaaccagat ttcacgcgct
    11881 tttttctgtc cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    11941 actttcgggc cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc
    12001 atgtgcagaa cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    12061 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    12121 tctttatagc cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta
    12181 tcgcctgcgc cagtcagaat gatcacgccg acgttgtcgt cataacgcgc atctgccagt
    12241 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    12301 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc aaaaccttcg
    12361 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    12421 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gataacgcca gcgggatttt
    12481 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    12541 ccaaggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    12601 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    12661 caagggttgc gccattattg ttactgcgca gcgccaccag ctcccggcgt tgatcgtcat
    12721 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    12781 tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcgcgt tgttcagcat
    12841 tttgcaaccc aggatgcccg ccttcgacaa caaccccaca aagccccggc atcccctggc
    12901 aagccgccat catcgccacc cgtcccccaa gcgagtaccc caccaaccag aagttaagga
    12961 tgttgtaact aaccagggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    13021 taatcgccgc cgaaccaccg tgacctggga gataaacata caaccgtgag tagtcggcaa
    13081 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    13141 gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    13201 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    13261 taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc gtttcaagtt cctgccagtt
    13321 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    13381 cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg agaaaatttg
    13441 cccgccgttg ttgttcacca caattaatat cagcggcgcg gaaacctgac gcaataacgc
    13501 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    13561 gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggccccacg
    13621 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    13681 caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
    13741 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
    13801 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    13861 gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc
    13921 gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg
    13981 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgttgca gttcgctggt
    14041 cgctttggca ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag
    14101 cacgtcgcca atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc
    14161 ttccgcactc atgcgcccgg caaccaccac gccgcgtttt tgtcgccaga agaaccagtc
    14221 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    14281 ccagtcgccc agccgctgtt gccagctaag cccggtgtcg tccatttcgc catacagcgg
    14341 ttcggcaaac gggcagttga tatggactcc accagaatga agcgtaccga gagcatggtc
    14401 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    14461 gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc
    14521 aatcagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc
    14581 ttcaatcagt gccggataga gatttgctac cgccgtaccg gaggtcacaa tcaccgccac
    14641 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    14701 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    14761 cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    14821 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    14881 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    14941 attactattc catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    15001 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    15061 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgctgt aatgagagat
    15121 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    15181 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    15241 tcacattatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    15301 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaaccgtt
    15361 ggcagatatc ttccaccacc agcatattct cgcgctggtt tttatcatcc gccatcagcc
    15421 actcacctaa ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg
    15481 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    15541 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    15601 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    15661 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    15721 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    15781 gcaagggctt gatactcacc agtgtggcga taaattctct tgccttaatc gcatcgtgct
    15841 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    15901 attccaggcg tggtaaaagt aaattgccct gcgacgggtc aaacgcattc agcccccaaa
    15961 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    16021 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    16081 agaattgcgg gtatgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    16141 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    16201 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    16261 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    16321 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    16381 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
    16441 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    16501 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
    16561 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    16621 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    16681 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    16741 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    16801 ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt
    16861 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg
    16921 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    16981 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    17041 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    17101 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    17161 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    17221 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    17281 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
    17341 agctactgca taccgccttt aaccctggga aactggacaa gatttttatc agtcaccttc
    17401 atggcgatca tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    17461 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    17521 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    17581 ttctcgatga tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt
    17641 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    17701 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    17761 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    17821 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    17881 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    17941 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggcaagc
    18001 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac ctgttacgtg
    18061 aatgcaggtc aattttcccg gcgactgaac tggcgaatga tttcaccgtg tttaacgttt
    18121 aactc
//
LOCUS       DAZOYS010000075        17995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOYS010000075 DAZOYS010000000
VERSION     DAZOYS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.075"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUN9_002209"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUN9_002209"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0695600.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUN9_002210"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUN9_002210"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0695601.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUN9_002211"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUN9_002211"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0695602.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUN9_002212"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUN9_002212"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0695603.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUN9_002213"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUN9_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0695604.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUN9_002214"
     CDS             complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUN9_002214"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0695605.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUN9_002215"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUN9_002215"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0695606.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11211
                     /gene="barA"
                     /locus_tag="ACHUN9_002216"
     CDS             8449..11211
                     /gene="barA"
                     /locus_tag="ACHUN9_002216"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0695607.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLDLEPELLELLDEMDNVARE
                     ASKILG"
     gene            complement(11443..12783)
                     /gene="gudD"
                     /locus_tag="ACHUN9_002217"
     CDS             complement(11443..12783)
                     /gene="gudD"
                     /locus_tag="ACHUN9_002217"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0695608.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12804..14144)
                     /locus_tag="ACHUN9_002218"
     CDS             complement(12804..14144)
                     /locus_tag="ACHUN9_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0695609.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14146..15498)
                     /gene="gudP"
                     /locus_tag="ACHUN9_002219"
     CDS             complement(14146..15498)
                     /gene="gudP"
                     /locus_tag="ACHUN9_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0695610.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15933..16382)
                     /locus_tag="ACHUN9_002220"
     CDS             complement(15933..16382)
                     /locus_tag="ACHUN9_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0695611.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16400..17182)
                     /gene="truC"
                     /locus_tag="ACHUN9_002221"
     CDS             complement(16400..17182)
                     /gene="truC"
                     /locus_tag="ACHUN9_002221"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0695612.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17182..17511)
                     /locus_tag="ACHUN9_002222"
     CDS             complement(17182..17511)
                     /locus_tag="ACHUN9_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0695613.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4379 a         4914 c         4292 g         4410 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 aaagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggatttgg agccggagct gctggaactg
    11161 ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct
    11221 tgtcgagtag caatcggctt tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg
    11281 tttatgccgg atgcggcgtt aacgccttat ccggcctaca aaaccaatta aattcaatga
    11341 attaaaaaat gatgtagacc tgatacatag cacaacaggc tattttgcgt ttagcatcag
    11401 tctcaagccg gctccagata gagccggttt tggttttctg tcttaacgca ccatgcacgg
    11461 gcgcttgtta tcgaacgtcc agccaggaat cagatactgc attcccatcg catcgtcacg
    11521 cgcgccaagc ccgtgtttct gatacagctc atgggctttc atcacttgat ccatatcgat
    11581 ttctacaccc agccccggtt tttccggcac ctgaaccaga ccgcctttga tctcaaacgg
    11641 ttctttggtc aagcgctgat tgccttcctg ccaaatccag tgcgtatcaa tcgcggtgat
    11701 tttacccggt gcagcggcgg caacatgggt aaacatcgcc agggaaatat cgaagtggtt
    11761 gtttgagtgt gaaccccagg tcaggccaaa ttcatggcac atttgcgcca cacgtaccga
    11821 accttgcata gtccagaaat gcggatccgc cagcgggata tcaacggatt gtaacgagag
    11881 cgtatggccc atctgccgcc agtcggtggc gatcatattg gttgcagtcg gcaggccagt
    11941 cgcgcgacgg aactctgcca tcacttcacg cccggagaaa ccttgctccg caccatacgg
    12001 atcttctgca taagccagcg aacctttcag gtatttacca attttaattg cttcgttcag
    12061 cgaccaggca ccgttaggat cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc
    12121 cacaatagac tcggcttctt cttccccggc cagtacaccg cctttcagtt tgaaatcgtt
    12181 gaagccatat ttttcatacg ccgcttccgc taggcgcacc accgcatccg gcgtcatcgc
    12241 ttcttcatga cgcaggcgat accagtcgca tgagtcatcc ggctggcttt gatacggcag
    12301 aggtgtggct ttgcgattac cgacgaagaa cagataaccg agcatttcga cttcgctacg
    12361 ctgttgacca tcgcccagca gcgatgccac gtttaccccc agatgctgcc ccagcagatc
    12421 cagcattgcc gcttctatcc cggtaactac atgaatagtg gtacgcaaat caaatgtctg
    12481 caaaccgcgc ccaccagcat cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt
    12541 tttgtattca cccagcgttt tacctaccac cagcggaatc gcatcttcca gcgttttacg
    12601 gattttctcg ccgccgggaa tttcccctac gccagtgtga ccggaattat ctttgataat
    12661 cacaatatta cgcgtaaaga acggtgcgtg tgccccactc agattcatca gcatactgtc
    12721 atgacccgcc accgggatga cctgcatttc agtaacaaca gacgtcgtaa attgagaact
    12781 catatttatg tcctttttca gaatcaatga cggccgaaaa cgggacgttt acggtcaaag
    12841 gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt tacgcgcacc gccaggcaga
    12901 cgtttatagg cctcatgtgc cttttgtacc tgttcccagt ccagttccac gcccagaccg
    12961 ggcgcatcag gaacggcaat ttttccgttt ttaatctcca gcggattttt ggtcaggcga
    13021 caatcgccct cctgccaaat ccagtgggta tcgatagcgg taggattacc cggtgccgcc
    13081 gcgcccacat gggtaaacat cgccagagag atatcgaaat ggttattaga atggcagccc
    13141 caggtcagcc cccagtcgtc gcaaagctgc gccacacgga ctgcaccgga aagcgtccag
    13201 aagtgcggat cggcaagtgg aatatctacc gcattgagca tcaccgcatg acccatttcg
    13261 cgccagttgg tggcgatcat gttagtcgcg acgggcaagc cggtcgcccg ccgaaactcc
    13321 gccataactt cacgaccgga gaaaccctgt tctgcgccgc atggatcttc ggcataggta
    13381 agaacatcat tcagcccttt gcatagagaa atggcttcat caagtagcca ggcaccgttg
    13441 ggatcaacgg taatccgcgc atcagggaag cgtttcttca atgcacgaac agtgtcgatt
    13501 tcttgctcgc caggtaacac cccgccctta agtttgaaat ctttaaagcc atagcgatcc
    13561 tgtgaggctt ccgccagacg cacaacggct tcgctgttca tcgctttctg atggcgcaac
    13621 tgataccact catggttgcc cggcgtattt tccagataag gaagatcggt tttggtccga
    13681 tcaccgatat aaaacagata accgaggacg gtaatagtct cgcgttgctt gcctggcccc
    13741 aacagttcgc agaccggaac attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc
    13801 agcgccgcca cggcattaac gcgcaattca aaagtccagg cacctttgcc gaaggtatca
    13861 aaatcggctg cctgattacc tttatgcacc tgctgaacca ctttattcag gcgcgcaact
    13921 tcctggccca gaaccatcgg aatagcatcg accagtgttt gataaatcac ctctccgccc
    13981 ggcgcttcac caatgccggt atgcccggcg ttatcggtga gtaccacaat attgcgggtg
    14041 aaatatgcgt tatgtgcccc accaatatta agcaacatgc tatcgtgccc ggcgaccgga
    14101 atgactttca tatcagtaat aacaggactg gattgtgtcg tcatcttatt gccccgcgac
    14161 aggtttcagc tcgatacgct tgatatcgcc caccagcacc aggtagctca gtaccgcgat
    14221 taaggcatga acaccaacat aaatcagcgc cccattaaag gagccagtcg tgccaacgat
    14281 ataaccaatt gcgattggcg tgacgatgcc agaaatgtta ccgaacatgt tgaacaggcc
    14341 accggaaaga ccgctgatct cttttggcgc ggtatctgcc attactgccc aacccagcgc
    14401 cccgatgccc ttaccgaaga aggccagcgc cataaagccg atgatcatcc actcaacgtt
    14461 gacgtagttg cagaacacca tcaccatcga cagcaacatg cccattacga tcggtgtttt
    14521 acgcgcaatg ttcagcgatc ccgtgcgacg catcagccag tcggaaataa tcccacccag
    14581 cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc acaaagcccg ctttcagaat
    14641 cgacatccca cgcgcctgca ccagataaac cggaaaccag gtaatgaaaa agtaagtcag
    14701 ggcgttgata cagtactgac cgatataaac gccgatcatc atccgtgacc cgagcagttg
    14761 tttgatctgc ccccacttca cgctgaacgg cactttaact ttggtgtttt gctgatccat
    14821 attgatcagc gcgccacccg cggcgatgta ctccagctct ttctgattta cccccggatg
    14881 ttgatttggc tcgtggatga ctttcaacca gataaagctg atgacaatcc ccagaccgcc
    14941 cataaagaag aagacgtgtg accagcccac ttcatgcgtc agccagccca taatcggcgc
    15001 gaagatcacc gttgcgaagt attgagcgga gttaaaaatc gacaccgccg ttcccctttc
    15061 ctgcgccgga aaccaggccg caacaatgcg actgttgccg gggaaagatg gcgcttcagc
    15121 aagcccgacc aggaagcgca gagtaaacag ggcaacgata atgccgaatc cactaaagat
    15181 atcgacgaag ccttgcagca aggtaaacat cgaccagata aagatcgacc agaagtagac
    15241 gcgttttgaa ccaaaacgat ccagcaacca accaccaggg atctgcccga taacataagc
    15301 ccatgagaag gcagagaaca catagcccat tcccacggga tcaaggccga tatctttggc
    15361 catttccgaa ccggcaatag aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat
    15421 aaacaacatc accactatcc agtaacgagc attcgtgcgt ttttccacac tgctcgcagc
    15481 ctgacttaaa gaactcattg ttgcactcct gaaaattcgc gttagccacg ctcactctgg
    15541 actgcgacat agccaggaaa tcagaggtga cgtagggtgt tttttgccgt ttttataggt
    15601 cgttcgccga atacggcgcg tgtttatatc tggcaatagc agtataaaaa gcgcgccata
    15661 gcggctcacc gtgcaacaac acaacattaa tgcgttcaat gaggcccgat tttggcatta
    15721 gccctggacg gtggaatcca cttcacggaa atgaaaacaa gaacaagaaa ggaagggtta
    15781 aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa
    15841 aaatccgcgc tatgaagcag tttttactgg catttgcctg aaaagattcg attcagcacc
    15901 gctaaaacga catttaccgc tcgctgaaca tatcaggaca acagcgtgcc ccactgttcg
    15961 acccacggat ttgattccgt ttccggttcc gggttctcac tggcgtcaat caacagcatt
    16021 tcgccaaccc gctgagcgct ctgttcctgc aacaaggcat cgaactgttt gccgccattg
    16081 cagaaattta cataactgct gtcgccgagc gcaatcacgc cataacgcag attcggctgg
    16141 aagcccagac tatctttgat tccctgaaag agcggcacaa tgctatcagg aaggtcgccc
    16201 tgcccggtcg tggacgtaac caccagaaca tacttatcct gataaggcag ccagtcgctt
    16261 aattcaggat cttcaaatac cgttgctttg tggccctgtg cggtcagaat tgcttctgct
    16321 tcttccgcca ctaacagcga atttccgtac atggtgccga caaaaatacc aatttccgcc
    16381 ataccgtttt ctccctggat taggaactta tctctccatc ctgacccgat ggcgcactga
    16441 actcaaccct ttcattttca ggaagcagac cgcgccagcc aaattgtgat aacgcctgca
    16501 tccaggtgtc gtccaggccc gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca
    16561 atgacaactg actggcatgt agcattaacc gctggaggcc aaaatgctca gcaccgctgc
    16621 gattctggcg taaatcgcca tgtttgctat cgccaataat cggatgacgc aaatgggcaa
    16681 gatgtcggcg aagctgatgt ttgcgtccgg ttttcggctc cagttccacc aggccgtagc
    16741 gcgtggtcgg gtaacgtccg gtcgctaccg gcatttctac ggtcgccaga ccgcgataat
    16801 gcgtcactgc tggctgcggg cctttatctt cgcgggcaaa tttatcagcg attttgtcca
    16861 gttcttccac cagtggataa tccagcaccg cttcttccat caaccagccg cgcacaatcg
    16921 catggtaacg tttctggatt tggtgctgtt caaactgttg tgccagcagc cgtccggcct
    16981 cgctggataa tcccatcaac aacacaccag aagtgggtcg gtccagacga tgagcagtaa
    17041 aaacatgctg tcctatctgg tcacgcacgg tttgcatgac cactactttc tcgtcgcgat
    17101 ccagccagct acggtgaacc agccagccgg agggtttatt taccgcaacc agccattcat
    17161 cctgatagag tatttccagc attagctcgc atcatccgca aaaagagcat ccagtttttc
    17221 caactcagcc agaataagcg cgcgttgcgg atggtccgtc gccagcgcca tttcataata
    17281 gggtgcaaca gcaaaagcgc ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg
    17341 cgggatcagc acccactgca accactccag tggttccatg gtgtccataa agaacggttg
    17401 ggtactatta aattgatgcg gctggggttc atcgtttcgc cagtgctgat gttcacgcag
    17461 taacgcttca agcgtctgca actggaaacg aacgcggtca tgagtggtca taaagcaact
    17521 tcaataagaa aaactgccgc gaaggatagc aggaataaaa aaagggagca ctgtattcac
    17581 agtgctcccg gttcgtttcg cagcattcca gctacttttg ttgctccctg ctcatccttg
    17641 acaacttttc ctctggcctt gcggccaatc gttcatcctg aactattgct tcctgctcac
    17701 accaccccga tgtgatactt catcctgaag tgtccctggc cttcctgacc caccgaatca
    17761 tcctgaccgg ttctcattct ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct
    17821 cttcgcttca tccagaagcc tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt
    17881 catcatcctg atgttcactt cgttgtctga ctccctgtcg acgaagatag aatcgtcttt
    17941 ttcgcgaagt cttacaaggc gcttacagac aatgccttaa ggaaaatttc atatg
//
LOCUS       DAZOYS010000076        17968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOYS010000076 DAZOYS010000000
VERSION     DAZOYS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.076"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(60..605)
                     /gene="syd"
                     /locus_tag="ACHUN9_002223"
     CDS             complement(60..605)
                     /gene="syd"
                     /locus_tag="ACHUN9_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0695614.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            673..1521
                     /gene="queF"
                     /locus_tag="ACHUN9_002224"
     CDS             673..1521
                     /gene="queF"
                     /locus_tag="ACHUN9_002224"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0695615.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1633..2997
                     /gene="ppnN"
                     /locus_tag="ACHUN9_002225"
     CDS             1633..2997
                     /gene="ppnN"
                     /locus_tag="ACHUN9_002225"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0695616.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            3553..4842
                     /gene="sdaC"
                     /locus_tag="ACHUN9_002226"
     CDS             3553..4842
                     /gene="sdaC"
                     /locus_tag="ACHUN9_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0695617.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            4900..6267
                     /gene="sdaB"
                     /locus_tag="ACHUN9_002227"
     CDS             4900..6267
                     /gene="sdaB"
                     /locus_tag="ACHUN9_002227"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0695618.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            6379..7134
                     /gene="xni"
                     /locus_tag="ACHUN9_002228"
     CDS             6379..7134
                     /gene="xni"
                     /locus_tag="ACHUN9_002228"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0695619.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(7239..8387)
                     /gene="fucO"
                     /locus_tag="ACHUN9_002229"
     CDS             complement(7239..8387)
                     /gene="fucO"
                     /locus_tag="ACHUN9_002229"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0695620.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(8415..9062)
                     /gene="fucA"
                     /locus_tag="ACHUN9_002230"
     CDS             complement(8415..9062)
                     /gene="fucA"
                     /locus_tag="ACHUN9_002230"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0695621.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            9609..10925
                     /gene="fucP"
                     /locus_tag="ACHUN9_002231"
     CDS             9609..10925
                     /gene="fucP"
                     /locus_tag="ACHUN9_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0695622.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            10958..12733
                     /gene="fucI"
                     /locus_tag="ACHUN9_002232"
     CDS             10958..12733
                     /gene="fucI"
                     /locus_tag="ACHUN9_002232"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR0695623.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            12842..14260
                     /gene="fucK"
                     /locus_tag="ACHUN9_002233"
     CDS             12842..14260
                     /gene="fucK"
                     /locus_tag="ACHUN9_002233"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0695624.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            14262..14684
                     /gene="fucU"
                     /locus_tag="ACHUN9_002234"
     CDS             14262..14684
                     /gene="fucU"
                     /locus_tag="ACHUN9_002234"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0695625.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            14742..15473
                     /gene="fucR"
                     /locus_tag="ACHUN9_002235"
     CDS             14742..15473
                     /gene="fucR"
                     /locus_tag="ACHUN9_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0695626.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(15517..16617)
                     /gene="rlmM"
                     /locus_tag="ACHUN9_002236"
     CDS             complement(15517..16617)
                     /gene="rlmM"
                     /locus_tag="ACHUN9_002236"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0695627.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(16610..17005)
                     /locus_tag="ACHUN9_002237"
     CDS             complement(16610..17005)
                     /locus_tag="ACHUN9_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0695628.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(17024..17941)
                     /gene="gcvA"
                     /locus_tag="ACHUN9_002238"
     CDS             complement(17024..17941)
                     /gene="gcvA"
                     /locus_tag="ACHUN9_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0695629.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         4420 a         4653 c         4382 g         4513 t
ORIGIN      
        1 cctggcgatc tcctacaggg tctgtaagag atctctcaca accagatggg gtatttggat
       61 tacagaagag gcttaagttg attaaggaat tccgcaagat ttgaggctaa atgcgtccgt
      121 ttacgtgttc ccagcgtctc tttgcacact tcaccggaga gattacacac cgagatgacc
      181 tctaactctt cttccagcgt cgcgataaat agcgtgggcg gcagttttag ccttttctga
      241 gtcaccagat gaccaatcag attttcctgc acccgacgga agtcgtcttc actccaggtt
      301 tgcagcaatg tcagtttgat atcgccaaac tgcgcgtgca tatccccggc aaactgagtc
      361 gtataaaagg tatgaattgt gggttgtatc acaatatcaa aagcgcgttc aaccgcgttt
      421 acattttgtt ccccggtaaa cggctggggt tgccagtata cggcatcttc ggtagttgaa
      481 ataatgcacg gtgaaggaac accgtacagt tcctcactta acggccagct tttatgctct
      541 tcatgccacg catcgcagta gcgtgcagta aaatctttca gggcttgtgc ggtcaaatcg
      601 tccactaatt tctctcttca cttaaaccag atacacttgt cgtttagttt atctggttta
      661 tgacggtgaa acatgtcttc ttatgcaaac catcaggcac ttgcgggcct gactcttgga
      721 aaatcaaccg attaccggga tacctatgac gccagcctac tgcaaggcgt tccacgcagc
      781 ctgaatcgcg acccgctggg tctgaaagcg gataacctgc cttttcacgg tacggatatc
      841 tggacgctgt atgaactttc ctggctgaat gcgaaaggtt tgccgcaggt cgctgtcggt
      901 catgttgaac ttgattacac cagcgtaaat ctgattgagt cgaagagttt taagctctat
      961 ctcaacagtt ttaaccagac gcgttttaat aactgggatg aggtgcgcca gacgctggag
     1021 cgcgacttaa gcacttgcgc tcagggtaag attagcgtgg cgttatatcg tcttgatgaa
     1081 ctggaaggcc agccgatagg tcattttaat ggcacttgca ttgatgacca ggatatcact
     1141 atcgataact atgaattcac tactgactat ctggagaatg ccacctgtgg tgaaaaagta
     1201 gtggaagaga cgcttgtcag ccacctgctg aaatcaaact gcctgatcac ccatcaacca
     1261 gattggggtt cgatccaaat tcagtatcgt ggacgccaaa ttgacagaga aaaactgctg
     1321 cgttacctgg tctcattccg tcatcacaac gagttccacg aacagtgcgt ggaacgcatc
     1381 tttaatgacc tgttacgctt ctgccagcca gaaaaattga gcgtttacgc acgttatacc
     1441 cgtcgtggcg gtctggacat taacccgtgg cgcagtaata gcgattttgt cccatcgacc
     1501 acaagactgg ttcggcaata aattttttct caattttgcg tgctggattc acgcagaagg
     1561 ttgtgaaagg acatcaggca gggctattgt aatcaaaggg aatgacgata ttcgtcccat
     1621 aaggagtttt tcttgattac acatattagc ccgcttggct ccatggatat gttgtcgcag
     1681 ctggaagtgg atatgcttaa acgcaccgcc agcagcgacc tctatcaact gtttcgcaac
     1741 tgttcacttg ccgtactgaa ctccggtagt ttgaccgata acagcaaaga attgctgtct
     1801 cgttttgaaa atttcgatat taacgtcctg cgccgtgaac gcggcgtaaa gctggaactg
     1861 attaatcccc cggaagaggc ttttgtcgat gggcgaatta ttcgcgcgtt gcaggccaac
     1921 ttgttcgcgg ttctgcgtga cattctcttc gtttacgggc aaatccataa caccgttcgt
     1981 tttcccaacc tgaatctcga caactccgtc cacatcacta acctggtctt ttccatcttg
     2041 cgtaacgctc gcgcactgca tgtcggtgaa gcgccaaata tggtggtctg ctggggcgga
     2101 cactcaatta acgaaaacga gtatttgtat gcccgtcgcg tcgggaatca gctgggcctg
     2161 cgtgagctga atatctgcac cggctgtggt ccgggagcga tggaagcgcc gatgaaaggt
     2221 gctgcggtcg gacacgcaca gcagcgttac aaagacagtc gttttattgg tatgacagag
     2281 ccgtcgatta tcgccgctga accgcctaac ccgctggtca acgaattgat catcatgcca
     2341 gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc acggcatcat tatcttccct
     2401 ggcggtgtgg gtacggcaga agagttgctg tatttgctgg gaattttaat gaacccggcc
     2461 aacaaagatc aggttttacc attgttcctc accggcccga aagagagcgc cgactacttc
     2521 cgcgtactgg acgagtttgt cgtgcatacg ttgggtgaaa acgcgcgccg ccattaccgc
     2581 atcatcattg atgacgccgc tgaagtcgct cgtcagatga aaaaatcgat gccgctggtg
     2641 aaagaaaatc gccgcgacac aggcgatgcc tacagcttta actggtcaat gcgcattgcg
     2701 ccagatttgc aaatgccgtt tgagccgtct cacgagaata tggctaatct gaagctttac
     2761 ccggatcaac ctgttgaagt gctggctgcc gatctgcgcc gtgcgttctc cggtattgtg
     2821 gcgggtaacg taaaagaagt cggtattcgc gccattgaag agtttggtcc ttacaaaatc
     2881 aacggcgata aagagattat gcgtcgtatg gacgacctgc tacagggttt tgttgcccag
     2941 catcgtatga agttgccagg ctcagcctac atcccttgct acgaaatctg cacgtaatct
     3001 ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt tggtcacata cccatcaatt
     3061 tatttcattt gttatatgaa tgtttcttac caccctcacg cgataaatat cattgcagtt
     3121 aatatgtcat acaatttatg ttgtaacgca aacgtttccc tattttcata aaccacattt
     3181 tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt ttttcagttc
     3241 aacctacata tattgcgcgc cccggaagaa gtcaaatgtc gtttaatggg caaatattgc
     3301 ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat ctatcttcca
     3361 agtagattat tgtatttggg atcaagatca ctgatagata cataacttgt gtgtatcttt
     3421 ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac agatttcatt
     3481 tttacggtca ggcaccttcc cgagctgaac tggctaaaag ctgaattatt tgcattcctc
     3541 caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt
     3601 gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc aatcggcgcg
     3661 ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct gatcatcatg
     3721 gctatccttg cgttcccgat gacatttttt gctcaccgcg gcctgactcg cttcgtactg
     3781 tctggtaaaa acccaggcga agacatcacc gaggttgtag aagaacactt tggtattggc
     3841 gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct gctggtttat
     3901 agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg tatgacacca
     3961 ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat cgttcgcttc
     4021 ggtgagcaga tgatcgttaa agcaatgagt attctggtat tcccgtttgt tggcgtactg
     4081 atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga aacgctgtct
     4141 ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct ggcaattccg
     4201 gttatggtgt tctctttcaa ccactctccg atcatctctt ctttcgccgt tgcgaagcgt
     4261 gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt cgcacacatc
     4321 atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct gactccggca
     4381 gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc taaccacttt
     4441 aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat caccaaatcc
     4501 ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg
     4561 cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc gctgttcatg
     4621 ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat gattgaaacc
     4681 ctgggcggcc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa
     4741 gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt cgtgatgggt
     4801 ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc gcgccgcttt
     4861 cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt attcgatatt
     4921 ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa agcgggtaaa
     4981 caatttaccg acgatctgat tgcccgtaat ctgcttaaag acgtgacccg cgtggtggtt
     5041 gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat cgccattatt
     5101 atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc tggttttatt
     5161 caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga agtggagttc
     5221 ccggttgatc agtgcatgaa cttccatgca gacaaccttt ctctgcatga aaacggtatg
     5281 cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta ctctattggc
     5341 ggtggcttta tcgttgatga agagcatttt ggccagcaga atagcgcacc ggttgaagtt
     5401 ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac cgggctgtca
     5461 ctctctggcc tgatgatgaa aaatgagctg gcgctgcaca gcaaagaaga gctggaacag
     5521 cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa
     5581 ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg ccggatgctg
     5641 gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat caacatgttt
     5701 gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc gccgactaac
     5761 ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagttcat ccgcgaagtg
     5821 aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc tctttataag
     5881 atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg tgtggcgtgc
     5941 tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca ggtgtgcatc
     6001 gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt cgccggacag
     6061 gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt gaacgccgca
     6121 cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt tatcgaaacc
     6181 atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg cggcggcctg
     6241 gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt gcgaggcctc
     6301 ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc ttatcgcctg
     6361 atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact gaatcttatt
     6421 cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca tgcgctcgat
     6481 cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga tgaaaaccgc
     6541 agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc gccaatgccg
     6601 gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg cggcgtcccg
     6661 tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc ggtcaaagtg
     6721 acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg tcagttactt
     6781 tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc gccatttatc
     6841 gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact tgcggggatc
     6901 agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac gcagctgctg
     6961 gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc cgaaaagtgg
     7021 cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat tgcccgctta
     7081 caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg gtaacggcga
     7141 gccggatgca aacgattatc aacacattga atttaatgca cttatatacc ggataagacg
     7201 cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
     7261 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
     7321 gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg caaatgtggc
     7381 ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt acgcgcctct
     7441 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
     7501 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcaacaccg
     7561 tgtggagtgt tgtaaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
     7621 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
     7681 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
     7741 agtgcatcgg ttagcgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
     7801 gcatcgacac ccgtcgcagc tttcagcgct ggaggcatac catccatcat gtcagcgtca
     7861 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttctct
     7921 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt ggtaggaatt
     7981 gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct acgcacatcg
     8041 gcaaactccg ggttgttgct gataatgcca atcgctttac aagtatcctg tggagaacca
     8101 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
     8161 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
     8221 gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt tttatcggtg
     8281 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
     8341 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
     8401 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
     8461 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcacg
     8521 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
     8581 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
     8641 gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
     8701 ttaccacctg ccgccgcaat catgtagtga atagcgggga tcggtcggtt aagaatggaa
     8761 actgctgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
     8821 taggccgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
     8881 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat gcctgtaggc
     8941 gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt
     9001 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
     9061 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
     9121 attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa ttcacaagct
     9181 tatattttgt gacctagctc aactaatcac agtaaataac tgcaagttct ctttttataa
     9241 ccccattaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tctattcacc
     9301 caagccagga atgcccgttt tcggcataaa caattaatac agtcatctgg tttgtgtttt
     9361 ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca
     9421 taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
     9481 gaaaatgaat ccatgagttc atttcagaca ggcaaatatt cactgatatg aagcccgaac
     9541 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
     9601 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
     9661 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
     9721 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
     9781 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
     9841 caattcctgc tgggatattg atgaaaaaac tcagttataa agcaggaatt attactgggt
     9901 tatttttata tgccctgggc gctgcattat tctggcccgc tgcagaaata atgaactaca
     9961 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
    10021 caaacccttt tgttacggta ttagggccgg aaagcagtgg tcacttccgc ttaaatcttg
    10081 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt
    10141 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
    10201 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
    10261 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
    10321 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgccta gcgcgtattc
    10381 gccactggcg ctgggcagta ttagcgcaat tctgctacgt tggcgcacaa acggcctgct
    10441 ggagctattt gattcgctac gctgtagaag aaattctagg tatgactgca ggctttgccg
    10501 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
    10561 tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc gctatggcac
    10621 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
    10681 gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag aatctcggcc
    10741 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
    10801 ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct gaactgatcc
    10861 ccacactctg cttcgcggtc atctttatct ttgcccgttt ccgctctcaa acggcaacta
    10921 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
    10981 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
    11041 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
    11101 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
    11161 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
    11221 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
    11281 gggctttaac ggcactgaac gtcccggcgc tgtttacctg gcagcggctc tggcagctca
    11341 cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg atgccgatga
    11401 cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
    11461 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
    11521 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
    11581 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
    11641 aatggcactg acctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
    11701 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcgat
    11761 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
    11821 atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact ggaccgatca
    11881 atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact ggaatggcgt
    11941 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
    12001 gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct actggtcacc
    12061 agaagcaatt gagcgtgtaa cggggcataa actcgatgga ctggcagaac acggcatcat
    12121 ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
    12181 aggtaacccg acgatgaagc cacactggga aatctctcag caagaggctg acgcttgcct
    12241 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
    12301 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
    12361 cctgggatcg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
    12421 tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg caccgcgcct
    12481 caccggtaaa gggccgttta cggatgtgta ctcggtaatg gcgaactggg gcgctaacca
    12541 tggggttctg accatcggcc acgttggcgc agactttatc actctcgcct ccatgctgcg
    12601 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
    12661 tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact acggtccgtt
    12721 gtacaagcgt taatacatta ctccctgatg tgatgcccgg tcgctccggc taccgggcct
    12781 gaacaagcaa gagtggttag ccggataagc aatgttatcc ggctatattg caggagcgat
    12841 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca gggccatcgc
    12901 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
    12961 gatggaaaac aacacctggc accagtggtc tttagacgcc attttgcaac gctttgctga
    13021 ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac
    13081 cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat
    13141 tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacagt tgatctccgc
    13201 acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt
    13261 ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctctttat
    13321 ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca cgatggccgg
    13381 aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac
    13441 cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct
    13501 acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg
    13561 tcacgatacc cagttcgccc tttttggcgc tggtgccgac caaaatgaac ccgtgctctc
    13621 ttccggtaca tgggaaattt taatggtccg tagcgcccag gttgatactt cgctgttaag
    13681 tcagtacgcc gattctacct gcgaactgga tagccaggca gggttgtata acccaggtat
    13741 gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac
    13801 accctggcaa atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa
    13861 aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac
    13921 cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa
    13981 tctacagatg ctggaaaaaa tcgggcactt taaagcctct gaattattgt tagtcggtgg
    14041 aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa
    14101 agttctcgac gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt
    14161 aggggaattt aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt
    14221 ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc
    14281 cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta
    14341 ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc cgcgctgatg
    14401 gcctgttggt gagcgacttg ctccaagcga ttatcccatt atttgagctg gacagttatg
    14461 ccccgccgct ggtgatgatg gcagcggtag aaggtgacac tctcgatcct gaagtagaac
    14521 gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc
    14581 gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag
    14641 cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat atcggtacgc
    14701 ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc
    14761 aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac
    14821 agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta
    14881 aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg
    14941 atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg
    15001 cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt
    15061 atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt
    15121 gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc
    15181 gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg
    15241 atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg
    15301 cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga
    15361 ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa
    15421 cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag
    15481 ggtagcaggc cgaagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc
    15541 caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg
    15601 ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag
    15661 attgtgtgat acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc
    15721 acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc
    15781 aaccatatcg cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc
    15841 ttcccgcagc cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc
    15901 gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc
    15961 gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat
    16021 aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa
    16081 tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt
    16141 atagcagcag cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata
    16201 gttcgccagc accccggcat cgcgcaaggc agcgcgtagc ggcacggtga atttacggca
    16261 gaatttcagc aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc
    16321 cttctctact acgccctgca acatgtcgac aatgggggta atacgatctt ctggcggcaa
    16381 atgctgcaag agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg
    16441 cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc
    16501 cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat
    16561 ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac
    16621 ttacgccctt gcactttaaa cggatagcac caactaacat taacgcccag cccgcgagga
    16681 agctcacgcc gccaaccggt gtgacaaatg cccacaaacg cagatgggac agcgccaggc
    16741 aataaaggct gccgctgaac aacaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa
    16801 accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa
    16861 acgcctggta ttcgaggccg gtctggatcc agcccatctc aacggccccc atggttttac
    16921 ttaacacatg cgcgccaaaa gcgcccagag ccacaaaaat gaagccgcta atggcggcga
    16981 aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa
    17041 agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta
    17101 ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcgttt ttactgacca
    17161 gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt
    17221 tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac
    17281 taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg gtatatgtct
    17341 gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct
    17401 tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt
    17461 acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa
    17521 catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg
    17581 gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga
    17641 gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg
    17701 ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca
    17761 ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga
    17821 tttgatgact tactgcggcc tgggtcacga aaagctcttc tgctgcgcga gtgaaactta
    17881 aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca
    17941 tggcttttaa actttgatgt taaattaa
//
LOCUS       DAZOYS010000077        17863 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOYS010000077 DAZOYS010000000
VERSION     DAZOYS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.077"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            148..826
                     /locus_tag="ACHUN9_002239"
                     /pseudo
     CDS             148..826
                     /locus_tag="ACHUN9_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            870..5720
                     /locus_tag="ACHUN9_002240"
                     /pseudo
     CDS             870..5720
                     /locus_tag="ACHUN9_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6088..7743
                     /gene="lldP"
                     /locus_tag="ACHUN9_002241"
     CDS             6088..7743
                     /gene="lldP"
                     /locus_tag="ACHUN9_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0695630.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7743..8519
                     /gene="lldR"
                     /locus_tag="ACHUN9_002242"
     CDS             7743..8519
                     /gene="lldR"
                     /locus_tag="ACHUN9_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0695631.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8516..9706
                     /gene="lldD"
                     /locus_tag="ACHUN9_002243"
     CDS             8516..9706
                     /gene="lldD"
                     /locus_tag="ACHUN9_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0695632.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9890..10363
                     /gene="trmL"
                     /locus_tag="ACHUN9_002244"
     CDS             9890..10363
                     /gene="trmL"
                     /locus_tag="ACHUN9_002244"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0695633.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10416..11237)
                     /gene="cysE"
                     /locus_tag="ACHUN9_002245"
     CDS             complement(10416..11237)
                     /gene="cysE"
                     /locus_tag="ACHUN9_002245"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0695634.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11317..12336)
                     /gene="gpsA"
                     /locus_tag="ACHUN9_002246"
     CDS             complement(11317..12336)
                     /gene="gpsA"
                     /locus_tag="ACHUN9_002246"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0695635.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12336..12803)
                     /gene="secB"
                     /locus_tag="ACHUN9_002247"
     CDS             complement(12336..12803)
                     /gene="secB"
                     /locus_tag="ACHUN9_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0695636.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12866..13117)
                     /gene="grxC"
                     /locus_tag="ACHUN9_002248"
     CDS             complement(12866..13117)
                     /gene="grxC"
                     /locus_tag="ACHUN9_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0695637.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13259..13690)
                     /locus_tag="ACHUN9_002249"
     CDS             complement(13259..13690)
                     /locus_tag="ACHUN9_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0695638.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13935..15479
                     /gene="gpmM"
                     /locus_tag="ACHUN9_002250"
                     /gene_synonym="pgmI"
     CDS             13935..15479
                     /gene="gpmM"
                     /locus_tag="ACHUN9_002250"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0695639.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15513..16772
                     /gene="envC"
                     /locus_tag="ACHUN9_002251"
     CDS             15513..16772
                     /gene="envC"
                     /locus_tag="ACHUN9_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0695640.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16776..17735
                     /locus_tag="ACHUN9_002252"
                     /pseudo
     CDS             16776..17735
                     /locus_tag="ACHUN9_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            complement(17722..>17863)
                     /locus_tag="ACHUN9_002253"
     CDS             complement(17722..>17863)
                     /locus_tag="ACHUN9_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0695641.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4481 a         4620 c         4673 g         4089 t
ORIGIN      
        1 aatgatacat ttttatgatg aatgcttaaa acaatattta atcgacacac atcactgtca
       61 ttaatgtaaa gcaacataga tcaccaataa tacaatggac aactctaccg gcattgatcg
      121 ctggtcggag gaaataagga tttttacatg aacttgaaga agattttttt cagtgctgtc
      181 actgtttcag ttctctgcgc attaacggga tgtgactaca ttgaagaagg caagccagaa
      241 agtagtttat tgaaacaaca agaagaacat taaaattgat ctacttgaaa aacaacaagc
      301 acagctaaag agtcaactgg aaactattca aaaacaacaa actggcataa taagcagtac
      361 aaagacttta actcacgtaa ttaaatccgt taaagatcaa caaaacactt ttatttttac
      421 cgagtttaac ccagcaaaaa cgaagtattt catcctgaac aatggttcgg tggctttagc
      481 gggtcgagtg ttatccattg acgccacaga gaatggcagt gttattcata tttcactggt
      541 caacttatta agtacaccta tctcaaatat tggttttaat gcgacatggg gtggtgaaaa
      601 acctgtcgat gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa
      661 atccacattg aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt
      721 atcgcccaat aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga
      781 caatcttccc tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg
      841 cttataacaa ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc
      901 ctgcgacagg gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg
      961 ggcgcagtaa gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg
     1021 cattggcgaa tgccgggaat gacaacggcc agggtgttga ttacggtagt ggatcagctg
     1081 gcgacagctg ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg
     1141 gttccagtac tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg
     1201 ggtcaaaaac ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag
     1261 aaggcgatag aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg
     1321 ccctcggccg ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca
     1381 aagcggaagg agcaaacgcc attgccctgg gaaatgccac taaagctact gagattatga
     1441 gtattgctct tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa
     1501 gtagcgtcgc atctgaagaa aacgcaattg ccctggggcg tagcagtgta gctagcggta
     1561 ctgacagcct cgcatttggc agacaatcac ttgccagcgc agcgaacgct attgcgatag
     1621 gtgctgagac cgaagccgct gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag
     1681 ggactaatag catggcaatg gggttcggaa gccttgccga taaagtcaat actatcgcat
     1741 taggaaatgg cagccaggct ctggcagata atgcaatcgc cataggctag ggcaacaaag
     1801 ctgatggcgt ggatgccatc gctctgggta atggtagcca gtcgagaggc ttaaacacca
     1861 ttgccttagg cacagccagt aatgcaactg gtgataagag tcttgcgctt ggtagtaata
     1921 gcagtgccaa cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca
     1981 ataccgtctc tgtcggcaat agttcattaa aacgcaagat cgttaatgtg aaaaatggcg
     2041 cgatcaagtc tgacagttac gatgccatta atggttcaca gctttatgcc attagcgact
     2101 cggtagcaaa aacgcttgga ggaggggctg cagtagatgt tgatgacggt actgttacag
     2161 caccaaccta caatttaaaa aatggtagca aaaataacgt aggggctgcg ctcgctgtac
     2221 ttgatgaaaa caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct gctcatggta
     2281 ctagtagccc aactgccagc gtaatcaccg atgttgcgga tggcacgatt tcagcctcca
     2341 gtaaggatgc ggttaacggt tcccaactga aagctaccaa tgacgatgtc gaagccaaca
     2401 ccgccaatat cgctactaat accagcaaca ttgccacgaa tacagcaagt attgccacca
     2461 ataccaccaa tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc
     2521 tctggaacga aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa
     2581 tcaccaacgt taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc
     2641 agctgaaaac caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt
     2701 ccaatattgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2761 gtgaggatgc gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg
     2821 agaccaccag caaaatcacc aacgttaaag atggcgacct gacgactggc agcaccgatg
     2881 ccgttaacgg ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata
     2941 tcgccactaa caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg
     3001 atgcgctgaa atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg
     3061 ccagcaaaat caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta
     3121 acggtagcca gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt
     3181 ctgtgaatac tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt
     3241 ataacgtcgg cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg
     3301 ctctgctttg ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg
     3361 caagcgtgat cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa
     3421 acggctcaca actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg
     3481 aagtcaatgc agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg
     3541 ataatgtcgg tgatgccctg aatgctatcg ataccactct tgacgacgct ctgctctggg
     3601 atgaggacgc cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca
     3661 gtgtaatcac taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg
     3721 gctcacaact ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag
     3781 tcaacgctga cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca
     3841 acgtcggtga cgccctggat gcgcttgatg ataacgccct gctgtgggat gagactgcca
     3901 atggcggtgc tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg
     3961 tcgctaatgg cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg
     4021 cgacgaatat gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg
     4081 acgcaaccta tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg
     4141 atttagcctt taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg
     4201 ctgtcgcgtc tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata
     4261 ccggtattgc gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg
     4321 acactagcgt aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc
     4381 taggtgcatt gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg
     4441 gttcagaagc ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg
     4501 ccactacgcc aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg
     4561 gtgaagactc gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta
     4621 tcggtttgaa cactctggtt ctagctgatg cgatcaacgg cattgctatc ggcagtaacg
     4681 cacgcgcaaa ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg
     4741 cacagactgg ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt
     4801 ctgtcggtag tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata
     4861 ccgatgcggt taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc
     4921 agagcattac taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg
     4981 aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg
     5041 atggcgtaga tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg
     5101 ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa
     5161 tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg
     5221 ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg
     5281 acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca
     5341 gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg
     5401 cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga
     5461 tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg
     5521 caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg
     5581 gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc
     5641 gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg
     5701 gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat
     5761 tgcctgatgc gttacgcgca tcaggcaaga taaggcgccc cgggtcaaca atctaagggc
     5821 aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc
     5881 ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca
     5941 atattgccaa tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa
     6001 ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg
     6061 atgtgcgtgg actccaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc
     6121 gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg
     6181 ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt
     6241 gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat
     6301 ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat
     6361 aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc
     6421 cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga
     6481 gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt
     6541 aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc
     6601 gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt
     6661 ggccagatgg tggggcggca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg
     6721 gcgattatgg acggctggcg cggtatcaag gagacgtggc ctgcggtcgt ggttgcgggc
     6781 ggctcgtttg ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac
     6841 attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcagcca
     6901 gtgcgcgtat tccgttttgg tgatttgggg gcgtcaaaga ttgatatgac gctggcccac
     6961 accggttaca ctgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgacagct
     7021 accgtaacac tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg
     7081 tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca
     7141 ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc
     7201 accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg
     7261 tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc
     7321 atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg
     7381 gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg
     7441 ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa
     7501 gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc
     7561 ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta
     7621 ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc
     7681 acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct
     7741 taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga
     7801 ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc
     7861 aactcggcgt atcacgtaat tcactgcgcg aggcgctggc aaaactggtg agcgaaggcg
     7921 tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg
     7981 agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg
     8041 atattctgga agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg
     8101 ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc
     8161 cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata
     8221 acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc
     8281 atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc
     8341 aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg
     8401 cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg
     8461 cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat
     8521 tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt
     8581 ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt
     8641 gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac
     8701 gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg
     8761 catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc
     8821 gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg
     8881 cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga
     8941 gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg
     9001 cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta
     9061 cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca
     9121 tgatttaggt aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg
     9181 ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga
     9241 tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc
     9301 agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg
     9361 tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc
     9421 cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg
     9481 tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc
     9541 gggtgtagct aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac
     9601 tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga
     9661 gttgcctacg gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc
     9721 tcaccctcag ggagagggga ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca
     9781 ccgcactctt tttttctccc tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg
     9841 cacggttcac caattttctg ctatcctgcc cccgcactaa gggggcagta tgctaaacat
     9901 cgtactttac gaaccagaaa ttccgccaaa tactggcaac atcatccgtc tttgcgctaa
     9961 taccggcttt cgtctgcata tcatcgaacc gatgggattt gcctgggacg ataagcgcct
    10021 gcgccgcgcg gggctggact atcacgagtt taccgccgtt acgcgtcatc atgactatcg
    10081 cgcgttcctc gaagcagaaa atccccagcg cctgtttgcc ctcaccacga aaggtacgcc
    10141 tgctcacagc gccgtaagct atcaggatgg cgattatctg atgttcggcc cggaaacacg
    10201 cggcctgcca gcgagcattc ttgatgccct gcccgctgaa caaaaaattc gcattccgat
    10261 ggtgccggac agccgcagca tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc
    10321 ctggcggcag ctgggatatc cgggagcgtt gttgagagat tagttactgt atgccggata
    10381 gatgattaca tcgcatccgg cacgatcaca ggacattaga tcccatcccc atactcaaat
    10441 gtatggttaa taccgttgaa atgctggtcc atatccattg atggcttatc gctgtctggt
    10501 ttaccgacaa tacgagccgg aacgccagcg gcggttgtat gcggcggcac cggttgcagc
    10561 accacggaac ctgcgccaat cttcgcgccg cgaccaactt caatattgcc gaggattttc
    10621 gcgcccgcgc caatcatcac accttcacga attttcgggt gacggtcacc accagattta
    10681 cccgtaccgc caagcgtcac agattgcaga atcgatacgt cgttttcaat taccgccgtt
    10741 tcacccacaa cgatgcccgt ggcgtggtcg agcataatgc cacggccaat ttttgctgcc
    10801 gggtgaatat cgacctggaa cgtcacagaa acctggtttt gcagaaagat tgccaatgcg
    10861 cgacgcccct gattccacaa ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc
    10921 ttcaggtata acaacggggt tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc
    10981 tgaatatcac aggccgcaga ggcgatcatt tccgggtcag cggcgtaggc ttcttccacc
    11041 acttcacgga tagcaatagc aggcataatt ggcgatgaca gcttgttcgc cagcatgtag
    11101 ctcagtgcac taccaaggtt ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc
    11161 attggctcac agtccgccag cgttctggct tcggctttaa tattgttcca gacaatttcc
    11221 agttcttcac acgacattgc ttactccaca cgatgagata atgaccggcc cgttctgcgc
    11281 gggccgggtc atagcggtaa caaaggttcc ctggggttag tggctgctgc gctcgtcctt
    11341 gcgtgcacga cctaataacg tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac
    11401 ttgataaatt tcctcggtta ttggcatttc tacgccgaag cgatgcgcca gttcgcggac
    11461 ttctttcgta ttgcggtagc cttccaccac ctgaccaatc ttctcctgcg cgctttgtac
    11521 atccatgccc tgaccgagca tcatgccaaa acggcggtta cgcgactggt tgtcggtaca
    11581 ggtaagcacc agatcgccca gccccgccat gcccataaag gtggcaggat cggcacccag
    11641 cgccgcgcca agacgcgaca tttcagccag cccacgggtg atcagtgccg tacgcgcatt
    11701 cgcaccaaaa ccgataccgt cggacatccc cgcgccaatg gcaatgacgt ttttcaccgc
    11761 accgccaagc tgcacaccga tgaaatccgg gttgctgtaa acgcggaaac ttttgccaca
    11821 gtgcagcaat tgttggagat catcggcaaa ggtctggtcg gtagaggcca acgaaatagc
    11881 cgtcggtaaa cctgccgcca gttctttcgc aaacgttggg ccagagataa ccgccagcgg
    11941 aatttgatcg cctaaggcct cacgcgccac gtcctgtaac agacgtccgg tttccgcttc
    12001 cagccctttg gtcgcccaca ccagacgcgc atcaggacgc atcagcggtt taatctggcg
    12061 cagcacttca ccaaagacat ggctgggtac gacgacgaga atattacggc tggctgccag
    12121 cgcagtggcg agatcgcttt caagatggag cgtatcggga aaaggcacat cggggagaaa
    12181 cgcggcgtta cagcggtcgc gttcaagcgt tgcgatatgt tcagggtcat ggccccagag
    12241 gacaacctcg tggccatttc ttgccagggt aatggcaaga gcggtgccgt acgagccggc
    12301 accgatcaca gtcattgaag cattacgttg gttcatcagg catcctgatg ttcttcagta
    12361 ccttcgccag cctgctgctg caaatagttc atgaacagcg catcgaagtt aatcggcgca
    12421 aggttcagtt gcgggaatgt accgcgggat accacgctgg tgatgcactc acgagcatac
    12481 gggaacagaa tgttcgggca gtatgcgccc aggcaatgtg ccatctgggt gccttcgata
    12541 cccgcgatgg agaaaatacc gccctgctga acttcacaca ggaacgcggt ttcttcgccc
    12601 agggaggccg ttacggtaac acgcagtacc acttcgtata cgtcatctgc cagttgggaa
    12661 gatgccgtat ccagatcaag tttaacttct ggttgccaat ctttctggaa aacgtgcggc
    12721 gcgttcggcg cttcgaaaga gatatccttg gtataaatac gttggatctg gaaagtcatt
    12781 tcagtgttgt tttgttctga catgtgtaga aaacccttaa gtgttgtcct taaatactgc
    12841 gcaatgtaat gccaacgttc acacgttatt tcagcagggg atccagtcca ccacgtgcat
    12901 ccaatgcata caagtcatca cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa
    12961 ccgtggtgcg accgctgcgt ttgatcatct cttcacgctt ggcggcattg ccatcgatcg
    13021 gcaactcctg aaaactcacg cccttgctgc tcagcagtgc ttttgcacga tggcaatacg
    13081 ggcaggtttc tttggtatag atttcaacat tggccataat ttagctcctg tttttttacc
    13141 ctgaaatttc atgttgcagg gcggctgcaa aatggagtgt ccccagaagc ttataacaat
    13201 aagtcgctgg gggcctaatt gcagctaacg gcctgcatca tgaaagacga caggtaaatt
    13261 atttgccgcg caccagaggc aagttttcgc cagcccagcc agcaacgccc tctttcagca
    13321 cgaatacttg cgcaaaacca gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc
    13381 cagaaccgtc taccacgata accggtttgt ctttgtgctt ctcaagctca ccaacattgt
    13441 tggctttgat ttcgctcggc aacagattga tagaacctgc gatatggcct ttacggaagt
    13501 catcacgctg acgtaaatcc acaaccacag cgtcttcttt gttgatcaga cgcgtagctt
    13561 caccacgagt aatcaccttc actttcgagg tcaggctctt aaacgtagtc acaagaaccg
    13621 ccaccagtaa cgcgatccag gcgatactca gtatgggatg acggccaaca aattgcataa
    13681 tttcttgcat ggggggtaac aactcccgac gtagtgatta aaaaaaccag gaaaggagta
    13741 tacctgcgcg gtgcggcaaa tacagccagc gcgttaactg gaatgcaatt ttgcggggca
    13801 cgacgaaaaa aaagcgcaaa tctggcatcg gcttgccgcg agcggtcgta tattttgatc
    13861 ttcagaggct attttatcga ttcagctgta gtaaaattac gcaaattttg actcttgagt
    13921 atgaggttgt cgcaatgtcg gtttctaaaa aacctatggt actggtgatt ctggatggct
    13981 atggctatcg tgaagaacag caggataacg ccatttttag tgctaaaacc ccggtaatgg
    14041 atgcactgtg ggccaatcgt ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc
    14101 tgcctgaccg tcagatgggt aactccgaag taggccacgt taacctgggt gccggccgca
    14161 tcgtgtatca ggacctgact cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta
    14221 atccagtgct gactggcgcg gtagataaag cgaaaaacgc aggtaaagca gtacacatta
    14281 tgggtctgct ctccgcaggc ggcgtacaca gccacgaaga tcacatcatg gcgatggtag
    14341 aactggcagc tgaacgcggc gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg
    14401 acaccccgcc gcgcagtgct gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc
    14461 tgggtaaagg ccgcgtagcg tccatcattg gtcgttacta cgcgatggac cgcgataacc
    14521 gttgggatcg cgttgaaaaa gcctatgacc tgctgactct ggcgcagggt gagtttcagg
    14581 ccgatactgc cgttgctggt ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg
    14641 tgaaagcgac cgttattcgt gcagaaggtc agccagatgc ggcaatggaa gacggcgatg
    14701 cgctgatttt catgaacttc cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga
    14761 acgctgattt cgatggcttc gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga
    14821 ccgaatacgc cgctgacatc aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca
    14881 ccttcggcga gtggatggcg aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa
    14941 aatatgccca cgttactttc ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag
    15001 atcgcattct gatcaactca ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct
    15061 ccgcagagct gaccgaaaaa ctggttgcgg ccatcaagag cggcaaatac gacaccatta
    15121 tctgtaacta tccgaacggc gacatggtag gtcacaccgg ggtgatggaa gcggcggtta
    15181 aagcggttga agcactggat cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg
    15241 gtggacaact gctgatcacc gctgaccacg gtaacgctga acagatgcgc gatccggcaa
    15301 cgggtcaggc acacacggca cacaccaacc tgccagttcc gctgatttac gttggtgata
    15361 agaacgtgaa agcggttgca ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc
    15421 tgatgggtat ggaaatcccg caagagatga ctggtaagcc gctgttcatc gtggaataat
    15481 ccctccccat gaggggaaag gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt
    15541 ttgcaatcag gcccatcatc tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct
    15601 tttccgccca cgcggatgag cgtgaccaac tcaaatctat tcaggccgat atcgccacaa
    15661 aagagcgcgc ggtacgccaa aagcaacaac aacgcgcaag cctgctcgca caattgaaaa
    15721 agcaggaaga agcgatctct gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca
    15781 atcaactcaa taaacagatt gatgagatga acgcgtcgat tgccaaactg gagcagcaaa
    15841 aagccgccca ggagcgcagc ctcgccgcac aactggatgc cgcattccgt cagggcgagc
    15901 ataccggtat tcagctaatt ctcagcggtg aagaaagcca gcgtggacag cgtttacagg
    15961 cttatttcgg ctatctcaac caggcgcgac aagaaaccat tgcccagttg aagcaaacgc
    16021 gtgaagaagt cgccatgcag cgtgctgaac tggaagagaa acaaagcgag caacaaacgc
    16081 tgttatatga acagcgcgcc caacaggcga aactgactca ggctctgaac gagcgtaaaa
    16141 agacgctggc ggggctggaa tcttccatcc agcaaggtca gcagcagcta agtgaactgc
    16201 gtgccaacga atcccgtctg cgtaacagta ttgcccgtgc ggaagccgcg gcgaaagcgc
    16261 gtgctgaacg agaagcgcgt gaggctcagg cagttcgcga caggcagaaa gaagcgacgc
    16321 gcaaaggcac cacctacaaa ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg
    16381 gcctgggtgc gccgcgtggt caggcattct ggccagttcg tggccctacg ctgcatcgct
    16441 atggcgagca gctacagggt gaattacgct ggaaaggaat ggttatcggt gcctctgaag
    16501 gtactgaagt taaagcaatt gccgacggtc gggtgattct ggctgactgg ctgcaaggct
    16561 acggtctggt ggtggtggtt gagcacggta aaggcgatat gagtctttac ggctataatc
    16621 aaagcgcgct ggtgagcgtt ggttcgcagg ttcgtgcggg ccagccaatt gcactggtgg
    16681 gcagcagtgg cggtcagggc agaccttcac tctatttcga aattcgccgc cagggtcagg
    16741 ccgtcaatcc acagccgtgg ttgggaagat aagttttgtt tccatttcgt cgtaacgttc
    16801 ttgcatttgc cgctctgttg gcgctctcct cccctgtaat tgctggcaaa cttgccatcg
    16861 tcattgatga ttttgggtat cgcccgaaca acgaaaacca ggtgctggcg atgccttccg
    16921 ctatctccgt cgctgtatta cccgattcac cgcacgcctg agagatggcg accaaagcgc
    16981 ataacagcgg tcacgaagtg ttgattcatc tcccgatggc accgttgagt aaacagccgc
    17041 tggagaaaaa tacgctgcgc ccggagatga gcagcgacga aattgagcgc attattcgta
    17101 gtgcggtcaa taacgtgccc tatgccgtgg ggatcaataa ccacatgggc agcaagatga
    17161 cctctaacct gtttggtatg cagaaagtga tgcaggcgct ggagcgttat aatctttact
    17221 tcctcgacag cgtaaccatt ggtaataccc aggcgatgcg cgccgcgcaa ggcactggcg
    17281 tgaaggtgat caaacggaag gtgttcctcg acgattcaca gaacgaagcg gacatccgtg
    17341 tgcaattcaa tcgcgcaatt gacctagcgc gtcgcaacgg atcaaccatt gccattgggc
    17401 atcctcaccc ttcaacggta cgcgtgttgc aacagatggt ttataacctg ccgccagaca
    17461 ttacgctggt gaaagccagc agcttgctga atgaaccgca ggttgatacg tctacaccgc
    17521 cgaaaaacac tgtgcccgac acaccgcgta atccgttccg tggcgtgaag ctgtgcaaac
    17581 cgaagaaacc gatagaacct gtttatgcta atcgcttctt tgaagtatta agcgaaagca
    17641 tcagccagag cacgctgatc gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc
    17701 ctgaagcggc gaagcttaac gctagcgtaa attaaaggcg ctacgcgtaa tgcgatggtg
    17761 cgaccgcgtt ttgtcgcgcc attgccataa gcggaaagac cacagtaacg cctgataacc
    17821 cacctttacg ctgcgcacat tggtaatcag gcgcttatac ata
//
LOCUS       DAZOYS010000078        17775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOYS010000078 DAZOYS010000000
VERSION     DAZOYS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.078"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(47..1021)
                     /gene="cysB"
                     /locus_tag="ACHUN9_002254"
     CDS             complement(47..1021)
                     /gene="cysB"
                     /locus_tag="ACHUN9_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0695642.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1231..3828)
                     /gene="topA"
                     /locus_tag="ACHUN9_002255"
     CDS             complement(1231..3828)
                     /gene="topA"
                     /locus_tag="ACHUN9_002255"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0695643.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4208..4459
                     /locus_tag="ACHUN9_002256"
     CDS             4208..4459
                     /locus_tag="ACHUN9_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0695644.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="ACHUN9_002257"
     CDS             complement(4495..5544)
                     /gene="sohB"
                     /locus_tag="ACHUN9_002257"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0695645.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5764..6522
                     /locus_tag="ACHUN9_002258"
     CDS             5764..6522
                     /locus_tag="ACHUN9_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0695646.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6519..7108
                     /gene="cobO"
                     /locus_tag="ACHUN9_002259"
                     /pseudo
     CDS             6519..7108
                     /gene="cobO"
                     /locus_tag="ACHUN9_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(7148..8020)
                     /gene="rluB"
                     /locus_tag="ACHUN9_002260"
     CDS             complement(7148..8020)
                     /gene="rluB"
                     /locus_tag="ACHUN9_002260"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0695647.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8233..9347)
                     /locus_tag="ACHUN9_002261"
                     /pseudo
     CDS             complement(8233..9347)
                     /locus_tag="ACHUN9_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9375..9995)
                     /locus_tag="ACHUN9_002262"
     CDS             complement(9375..9995)
                     /locus_tag="ACHUN9_002262"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0695648.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9992..10873)
                     /gene="rnm"
                     /locus_tag="ACHUN9_002263"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(9992..10873)
                     /gene="rnm"
                     /locus_tag="ACHUN9_002263"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0695649.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11011..11055
                     /gene="trpL"
                     /locus_tag="ACHUN9_002264"
     CDS             11011..11055
                     /gene="trpL"
                     /locus_tag="ACHUN9_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0695650.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11032..11125
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11147..12709
                     /gene="trpE"
                     /locus_tag="ACHUN9_002265"
     CDS             11147..12709
                     /gene="trpE"
                     /locus_tag="ACHUN9_002265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0695651.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12709..14304
                     /gene="trpD"
                     /locus_tag="ACHUN9_002266"
     CDS             12709..14304
                     /gene="trpD"
                     /locus_tag="ACHUN9_002266"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0695652.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14305..15666
                     /gene="trpCF"
                     /locus_tag="ACHUN9_002267"
     CDS             14305..15666
                     /gene="trpCF"
                     /locus_tag="ACHUN9_002267"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0695653.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15678..16871
                     /gene="trpB"
                     /locus_tag="ACHUN9_002268"
     CDS             15678..16871
                     /gene="trpB"
                     /locus_tag="ACHUN9_002268"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0695654.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16871..17677
                     /gene="trpA"
                     /locus_tag="ACHUN9_002269"
     CDS             16871..17677
                     /gene="trpA"
                     /locus_tag="ACHUN9_002269"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0695655.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4247 a         4725 c         4453 g         4350 t
ORIGIN      
        1 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
       61 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      121 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      181 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      241 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      301 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      361 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      421 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      481 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      541 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      601 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      661 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      721 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      781 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
      841 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
      901 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
      961 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1021 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1081 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1141 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1201 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1261 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1321 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1381 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1441 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1501 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1561 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1621 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1681 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1741 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1801 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     1861 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     1921 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     1981 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2041 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2101 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2161 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2221 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2281 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2341 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2401 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2461 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2521 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2581 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2641 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2701 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2761 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     2821 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     2881 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     2941 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3001 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3061 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3121 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3181 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3241 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3301 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3361 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3421 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3481 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3541 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3601 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3661 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3721 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3781 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     3841 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     3901 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     3961 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4021 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4081 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4141 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4201 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4261 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4321 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4381 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4441 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4501 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4561 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4621 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4681 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4741 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4801 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     4861 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     4921 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     4981 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5041 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5101 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5161 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5221 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5281 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5341 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5401 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5461 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5521 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5581 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5641 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5701 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5761 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     5821 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     5881 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     5941 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6001 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6061 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6121 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6181 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6241 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6301 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6361 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6421 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6481 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6541 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6601 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6661 cggcaacacg cgcagttggt cacggaaaaa agtgggcgtc gtgcagttta ttaaaggcac
     6721 ctggcctaat ggcgaacgca atctgctgga gccacatggc gttgagtttc aggtgatggc
     6781 aacgggcttt acctgggata cacaaaaccg cgagtctgat accgccgcct gccgcgaagt
     6841 ctggcaacat gcaaagcgga tgcttgctga ttcctcactg gatatggttt tgcttgatga
     6901 actgacgtat atggtggcgt atgactattt gccactggaa gaagtggtgc aggcgttaaa
     6961 tgaacgtcca catcaacaga cggtgattat cacgggtcga ggctgtcatc gggatattct
     7021 tgaactggcg gatacggtaa gtgagctgcg ccccatcaaa catgcgtttg atgccggtgt
     7081 taaggcgcaa atagggattg attattaatc tgtaaaaatg ccggatatga cgtccggcat
     7141 actttgatta accgttatta cgcccgccag aacgacgacc gccgctcacc tgactgtgac
     7201 gtttcaccgc acgacgaatc tgattagcct tcatgcgacg acggtctttt tctacagcga
     7261 ctttagagct ggtttctggc ggaagctcca ccagttcgcg cagatagtta gtctgggcga
     7321 ggtccagctc cgtccagcca ccgcgtggca gaccttttgg cagtgggata tcaccgtaac
     7381 gaacacggat caggcggctc acctgcacac caaccgcttc ccacagacga cgaacttcac
     7441 ggttacggcc ttcggtcaga gtcacgttgt accactggtt gatcccttcg ccgccgctga
     7501 acttgatggt tttaaaagct gccggaccat cttccagttg cacgccacga ctcaaatcac
     7561 gcagtttcgc gtcgtcaacc tgaccaaata cacgcacggc gtattcacgt tcaacttcac
     7621 ggcttggatg cattaaacgg tttgccagtt caccatcggt ggtgaacagc agcagaccac
     7681 aggtgttaac gtccagacga cctacggcaa tccagcgtgc gccacgcagc tttggcagac
     7741 ggtcaaacac cgtcggacgc ccttccggat cgttacgggt acacaactca ccttccggtt
     7801 tgtaataggc cagaacgcga caaatttgtt cagcggactc acgtaccgaa atcagatgac
     7861 catcgatacg aattttcagc ccaggggtaa cttcaacgcg atcgccgagt ttagcaattt
     7921 tgccatcaac actcacacga ccggcttcaa taatagattc gatttcacgg cgggagccgt
     7981 ggccagcgcg ggccagcact ttctgtaatt tttcgctcat agagcttcct tttggtgtcg
     8041 ccttcccagg cgtctgatat gcttttgtgc aacagtgagt cagaacttac atctgactca
     8101 ctgatagtaa agtctgtttg aactcttcag gccttaagta aacgactcga cgttttaact
     8161 ggcctggctt tgaggcgcaa catactacac tatctttatg gttaaagctt gtttcgatta
     8221 acatcagctt aattaccagc caccaccgcc tccgccaccc gcgccgccac cagcaccacc
     8281 accggaataa cttcgcccca acaaactggt tttggccccc agactcaggt gcatctgtaa
     8341 tgaaaacaaa acgccactac gggcaatttc cggggcgcta acagccgctg tgagtgatgg
     8401 ggtgatacgt gctacccatt ctcttccaag gtttgctgag actgcccaac ctagcaacgc
     8461 gtaatccagt ttccgtgttt cccctttccg ccgacgccca ctgtgtgttg ctgcctcttt
     8521 acgcgcaaga tagttaacga tagcttcacc gtgggcataa cgttgctgcc cagtttgtgt
     8581 aaactttggc agaaaaatat accccatgcc agtgagataa ccggtcagga aaacagcgct
     8641 aaaataaccc gctggcatat accagtaaaa gaatacatct ccgacattga tgtagagaga
     8701 atagaaggcc acgcctgaac aaatcagtgg taaacaattc ttccgtcctc aaggatgcgg
     8761 ccatttcgat atgtgtcgcc ctcttctcca gtaaagagat cgatttcgct aaatggatta
     8821 cccgcgatat cagggaactt gagtgaaaat cgggtcttat agatttcaaa cggccagtgg
     8881 ttaccagtca cgttccagat taacagatct gaatctccct cacgcaggaa agcattttta
     8941 acctgataat gaatttgata atgatagtcg ccttgaggca agaaacgttg tttatcgccc
     9001 acgacaatac tcattaaccc gatatgccag tcatgatgcc agggttcagg aataccatca
     9061 cgggtgacgc caacaatatg atagttcagc agtgcagcgt ccccatcagg ctgcatccag
     9121 agtcgtggta aatcacgata aaatccgtgt ttaatctgct gatttcgcgc ctgaaaatga
     9181 atattttctt tgatgtcgag caccccatcc ggttgcagag tggcttcaac atcaaagagc
     9241 gaaatccctt cttcataaga agggatctcc gtgttttctg cataacttag agatgataca
     9301 aatagccctg ctatcaacag caaaatgcag cgaaacttcc ctgccatagt cttccatccg
     9361 tggaacgcaa tttgttataa gaaaggcttc acatcaccta cgccttcacg caccacgacc
     9421 ggcgtatcat cggtaagatc aataaccgtt gtcggtttct gcccgagata gccgccgtga
     9481 ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc ggattcggta
     9541 aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag tgcttcaagc
     9601 aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg tttttcctgc
     9661 aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt gcccggcgtg
     9721 ttgtttttca ttaaacgaaa cgcaacgtta tcaacaaatg aataggtcga gagttcagaa
     9781 agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat acgacaaata
     9841 cgctccatcg cgtttttatc ttcaatttta cagccgagcg catagccgga atcagttgga
     9901 taaacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag acgttgctgt
     9961 gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg gtgttctgcg
    10021 gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt ttacgaccta
    10081 gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat aaatgatgct
    10141 gacgcgcaag ggccgccagc tgggtacgtt cattgggcga ttgctggcac tgcgcgactt
    10201 ccatcgcgtc accgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc catttagcac
    10261 taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga tgaatgacat
    10321 caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc ccgcgcgcca
    10381 gatatttttt aaagacatcc gccattgaac tggctttgcc gcaccccaca aggaaacgcg
    10441 caaaatgacc gcgcgtcacc gcaccgccct gcgccagtcg tcgcgcgcct tccagtgccc
    10501 ctggtatttg cgctttttca agtctttcgg caatcagttg cgcccgctga ttgcgccgtt
    10561 ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata ttcagcccga
    10621 caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga ataagattca
    10681 gcgccagtcc cgaacgtgaa atttcctctc ttgctggcac gattgcagct gtgttgtcat
    10741 ggtcggtgat cgccagggta ccgacgcgca tctcgactgc acggtgcacc aatacttctg
    10801 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcgtaat
    10861 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataaca
    10921 gttctggcaa atattctgaa atcagctgtt gacaattaat catcgaacta gttaactagt
    10981 acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact gaaagcttgg
    11041 tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca gatacccagc
    11101 ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc aaacacaaaa
    11161 accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga ctgcgctttt
    11221 tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag atatcgacag
    11281 caaagatgat ttaaaaagcc tgctactggt agacagtgcg ctgcgcatta cagcattagg
    11341 tgacactgtc acaattcagg cactttccgg caacggcgaa gccctgctgg cactactgga
    11401 taacgccctg cctgcgggtg tggaaaatga acaatcacca aactgccgtg tgctgcgctt
    11461 cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt cggtttttga
    11521 cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag aagcaatgtt
    11581 cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac cgcaactgtc
    11641 agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga tggtgattga
    11701 ccatcagaaa aaaaacaccc gcattcaggc cagcctgttt gctccgaatg aagaagaaaa
    11761 acaacgtctc actgctcgcc tgaacgatct tcgccagcag ctgaccgaag ccgcgccgcc
    11821 gctgccggtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg atgaagagtt
    11881 cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt tccaggtggt
    11941 gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt acgtgctgaa
    12001 aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca ccctgtttgg
    12061 cgcgtcgccg gaaagttcgc tcaagtatga cgccaccagc cgccagattg agatctaccc
    12121 gattgccgga acacgtccac gcggtcgtcg tgccgatggc tcgctggaca gagacctcga
    12181 cagccgcatc gaactggaaa tgcgtaccga tcataaagag ctttctgaac atctgatgct
    12241 ggtggatctc gcccgtaatg atctggcacg catttgcacc cccggcagca gctacgtcgc
    12301 cgatcttacc aaagttgacc gttactctta cgtgatgcac ctggtctccc gcgtggtcgg
    12361 tgagctgcgc cacgatctcg acgccctgca cgcttaccgc gcctgtatga atatggggac
    12421 gttaagcggt gcgccgaaag tacgcgccat gcagttaatt gccgaggcgg aaggtcgtcg
    12481 ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc tcgacacctg
    12541 cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag ccggtgctgg
    12601 cgtagtcctt gattctattc cgcagtcgga agccgacgaa acccgtaata aagcccgcgc
    12661 tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat ggctgacatt
    12721 ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt gcgcagcaat
    12781 gggcataatg tggtgattta ccgcaaccat attccggcgc agaccttaat tgaacgcctg
    12841 gcgacgatga gcaatccgat actgatgctt tctcctggcc ccggtgtgcc gagcgaagcc
    12901 ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat tggcatttgc
    12961 ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc gggcgaaatt
    13021 cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc cggattaaca
    13081 aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc ggccggttta
    13141 accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc ggatcgcgtt
    13201 tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg cctgctggaa
    13261 caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca accgattctg
    13321 gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct gttttcagcg
    13381 gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag catgaaaatt
    13441 cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga aaacgcagcg
    13501 ccgttcccgc gcccagatta tctgtttgct gatatcgtcg gtactggcgg tgacggcagc
    13561 aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg gctgaaagtg
    13621 gcgaaacacg gcaaccgtag cgtctccagt aaatctggtt cgtccgatct gctggcggcg
    13681 ttcggtatta atcttgatat gaacgccgat aaatcgcgcc aggcgctgga tgagttaggt
    13741 gtttgtttcc tctttgcgcc gaagtatcac accggattcc gccacgcgat gccggttcgc
    13801 cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa cccggcgcat
    13861 ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat tgccgaaacc
    13921 ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat ggatgaagtt
    13981 tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcgaaattaa gagctatcaa
    14041 ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc aggcggaaca
    14101 ccggaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga cgccgcccat
    14161 gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca tgaagatctg
    14221 caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta cgacagagtt
    14281 accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat cgtcgcagac
    14341 aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt tcagaatgag
    14401 gttcagccga gcacgcgaca tttttatgat gcgctacagg gtgcacgcac ggcgtttatt
    14461 ctggagtgca agaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt cgatccggca
    14521 cgcattgcct ccatttataa acattacgct tcggcaattt cggtgctgac tgatgagaaa
    14581 tattttcagg ggagctttga tttcctcccc atcgtcagcc aaatcgcccc gcagccgatt
    14641 ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta ttaccaggcc
    14701 gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca gcttgcagcc
    14761 gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga ggaactggag
    14821 cgcgccattg cattgggggc aaaggtcgtt ggcatcaaca accgcgatct gcgtgatttg
    14881 tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa cgtgacggta
    14941 atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca cttcgctaac
    15001 ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc cgtgcgccgg
    15061 gtgttgctgg gtgagaataa agtatgtggt ctgacgcgtg ggcaagatgc taaagcagct
    15121 tatgacgcgg gcgcgattta cggtgggttg attttggttg cgacatcacc gcgttgcgtc
    15181 aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt tggcgtgttc
    15241 cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct ggcggcagtg
    15301 caactgcatg gtaatgaaga tcagctgtat atcgatacgc tgcgtgaagc tctgccagca
    15361 cacgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg cgagcttcag
    15421 cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg tttcgactgg
    15481 tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt aggcgcagat
    15541 aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc tgctgtagag
    15601 tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac gctgcgcgca
    15661 tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag tttggcggca
    15721 tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct tttgtcagcg
    15781 cgcaaaaaga tcctgaattt caggcccagt tcaacgacct actgaaaaac tatgccgggc
    15841 gtccaaccgc actgaccaaa tgccagaaca ttacggctgg cacgaacacc acgctgtatc
    15901 tgaagcgcga agatttgctg cacggcggcg cgcataaaac taaccaggtg ctcggtcagg
    15961 ctttactggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggt gccggtcagc
    16021 atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt atttatatgg
    16081 gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta atgggtgcgg
    16141 aagtgatccc ggtgcatagc ggttccgcga cgctgaaaga tgcctgtaac gaggcgctgc
    16201 gcgactggtc cggtagttac gaaaccgcgc actatatgct gggcaccgca gctggcccgc
    16261 atccttatcc gaccattgtg cgtgagtttc agcggatgat tggcgaagaa accaaagcgc
    16321 agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt ggcggcggtt
    16381 cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc ctgattggtg
    16441 tggagccagg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta aaacatggtc
    16501 gcgtgggtat ctatttcggt atgaaagcgc cgatgatgca aaccgaagac gggcagattg
    16561 aagaatctta ctccatctcc gccggattgg atttcccgtc tgtcggccca caacacgcgt
    16621 atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa gcccttgaag
    16681 ccttcaagac gctgtgccta cacgaaggga tcatcccggc gctggaatcc tcccacgccc
    16741 tggcccatgc gctgaaaatg atgcgcgaaa acccggaaaa agagcagcta ctggtggtta
    16801 acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg aaagcacgag
    16861 gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc gcaaagaagg
    16921 cgcattcgtt cctttcgtca cgctcgggga tccgggcatt gagcagtcat tgaaaattat
    16981 cgatacgcta attgaagccg gtgctgacgc gctggagtta ggcatcccct tctccgaccc
    17041 actggcagat ggcccgacga ttcaaaacgc cactctgcgc gcctttgcgg caggtgtgac
    17101 tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga ccattcccat
    17161 tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt tttatgccca
    17221 gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccaattg aagagtccgc
    17281 gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct gcccgccaaa
    17341 tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca cctatttgct
    17401 gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca atcatctggt
    17461 tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta tttccacccc
    17521 ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg gttcagccat
    17581 tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg cactgaaagt
    17641 ttttgtacaa ccgatgaaag tggcgacgcg cagttaatcc cccagccgcc agttccgctg
    17701 gcggcatttt aactttcttt aacgaagccg gaaaaatcct aaattcattt aatatttatc
    17761 tttttaccgt ttcgc
//
LOCUS       DAZOYS010000079        17641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOYS010000079 DAZOYS010000000
VERSION     DAZOYS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.079"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUN9_002270"
     CDS             <1..216
                     /locus_tag="ACHUN9_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0695656.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHUN9_002271"
     CDS             complement(161..298)
                     /locus_tag="ACHUN9_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0695657.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHUN9_002272"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHUN9_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHUN9_002273"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHUN9_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..1586
                     /locus_tag="ACHUN9_002274"
                     /pseudo
     CDS             696..1586
                     /locus_tag="ACHUN9_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1589..2122
                     /locus_tag="ACHUN9_002275"
     CDS             1589..2122
                     /locus_tag="ACHUN9_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0695658.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="ACHUN9_002276"
     CDS             complement(2151..2678)
                     /locus_tag="ACHUN9_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0695659.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..5436)
                     /locus_tag="ACHUN9_002277"
     CDS             complement(2680..5436)
                     /locus_tag="ACHUN9_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0695660.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQET
                     VNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            complement(5588..6187)
                     /locus_tag="ACHUN9_002278"
     CDS             complement(5588..6187)
                     /locus_tag="ACHUN9_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0695661.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(6255..9689)
                     /locus_tag="ACHUN9_002279"
                     /pseudo
     CDS             complement(6255..9689)
                     /locus_tag="ACHUN9_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(10033..10713)
                     /locus_tag="ACHUN9_002280"
     CDS             complement(10033..10713)
                     /locus_tag="ACHUN9_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0695662.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(10611..11354)
                     /locus_tag="ACHUN9_002281"
     CDS             complement(10611..11354)
                     /locus_tag="ACHUN9_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0695663.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAALACM"
     gene            complement(11365..12063)
                     /locus_tag="ACHUN9_002282"
     CDS             complement(11365..12063)
                     /locus_tag="ACHUN9_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0695664.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(12063..12392)
                     /locus_tag="ACHUN9_002283"
     CDS             complement(12063..12392)
                     /locus_tag="ACHUN9_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695665.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(12389..14962)
                     /locus_tag="ACHUN9_002284"
     CDS             complement(12389..14962)
                     /locus_tag="ACHUN9_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0695666.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(14943..15356)
                     /locus_tag="ACHUN9_002285"
     CDS             complement(14943..15356)
                     /locus_tag="ACHUN9_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0695667.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(15383..15814)
                     /locus_tag="ACHUN9_002286"
     CDS             complement(15383..15814)
                     /locus_tag="ACHUN9_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0695668.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(15828..16580)
                     /locus_tag="ACHUN9_002287"
     CDS             complement(15828..16580)
                     /locus_tag="ACHUN9_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695669.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(16588..16983)
                     /locus_tag="ACHUN9_002288"
     CDS             complement(16588..16983)
                     /locus_tag="ACHUN9_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0695670.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(16980..17555)
                     /locus_tag="ACHUN9_002289"
     CDS             complement(16980..17555)
                     /locus_tag="ACHUN9_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0695671.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(17570..>17641)
                     /locus_tag="ACHUN9_002290"
     CDS             complement(17570..>17641)
                     /locus_tag="ACHUN9_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0695672.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         3831 a         5204 c         4260 g         4346 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agcgcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaaatcc cccatctgtt ttaaaaatga aatcaccacc
     3001 accatctgaa ccgttattaa tatgaacacc atcaccaccg ttatctttcc acaaaaacat
     3061 acgaattctt ccgtcttcgt tggcgaatgt aatgtggtgg cggtaattac tttttaatct
     3121 aaaattgctg ccattagatt caatatcttt ataaagatgt aatcctaact catcaatatg
     3181 tccgatgcgc gaaccatttg aatagaagct aataatacca tctccatctt gcctaaatcc
     3241 ggtgtcgtta tctcccagaa caatggagtt ttcacccagt gcgttatcag tagctccaat
     3301 agctagacct cctttaattt tggccccgtg gctgacagat atagcacctg ttctcagatt
     3361 tatagcgaat ggccttaatg ggccgatatt accgttttcg ccttgtcctt cagccgtcgg
     3421 aatgaaatga agaaaatctt ctgaacgacg gaaaattaga ccgaaggtat cattgaaaat
     3481 tcgcagcgca ttcaccgtgc caattttcag ttctccggtc attttatcgc cggaacgctg
     3541 aacggcgtta ccagccttgt ttaccgtttc ctgtaaaccg aggtattcga taacggcagc
     3601 aacggtcgat ttcgcaagaa tatcccgccc gacttttgtc agggttgcca ggctggcgac
     3661 atcattcccc gtaaaatacg gaaacttgcc tgccgcagta gtaagtccgg ccagcgccgt
     3721 cagggtggca tctttcggtt gcttacccgc aagcgcgtta gtcatggtgg tcgcaaaatt
     3781 cgggtcgttg cccagcgccg cagccagctc gttcagcgtg ttcagtgcat caggtgacga
     3841 atctacaagg gcggcaatcg cagccataac gaaagccgtg cttgcgattt gggtattatt
     3901 cgttccctgt cgcgcagttg gtgttgttgg cgttccggtc agtgcaggac tatttaaggg
     3961 cgctttcttg ttcgtttcac ccattaccgc cttaaccgct tttggcgttg ccgccagtga
     4021 ctcggaagtg ctgttggtcg cactgctgag ctgtactatc ccctttttcg tcgtgctcgc
     4081 atcctccagc gccacggcgg atgcaatatc ctctgcccgt tttgctgctg tctcggcgcg
     4141 cgttgccgcg gattcagcag caactttgct ctgagatgca gccgtcgcac tgcctgccgc
     4201 ctctgatgct ttcgttgttg ctgtcgtggc actacctttc gctgctgacg cttgtctggt
     4261 cgcctcatct tttgaagcag acgcagatga tgccgatgac gccgctgaac tggctgacga
     4321 tgcggctgct gccttagagg aagcagcatt gtctgctgaa gtctttgcat ttgtttcaga
     4381 ggtttttgct gcagaagcag acctcgctgc tgcagtggct tgctcagtgg ctttgccagc
     4441 cttcgttgtg gctgttgaag cggatgatgc ggcgctttct gccgattttc cggcggcggt
     4501 ggcactggct gaggcctgcc cggcacttgt tgacgcggca ctggcagata atgcagccgc
     4561 tgtttttgaa cctgccgcag ctgaggcgct ctgtcccgct gctgtttcag aagacttagc
     4621 gttcgtctcg gacgtttttg ccgccttcgc ggaatttcct gccgccgttg ccgaggaagc
     4681 tgcactactg gcacttgatg atgcattcgt ttctgaagat ttcgctgcct cttttgaggc
     4741 cgccgcaccc cgtgccgagg tggcagcttc tgacgccttc gtggtcgctg tggatgcaga
     4801 agtggctgcc gatttttgtg atgctgcggc attcgtttct gacgttttcg cggcactggc
     4861 gctggtagct gccgcgcttt ttgatgactc tgcagcagca ctttttgctg cttcacgggc
     4921 ctttgtcgat gccgttcctg cgctggaaga cgctgactga gccgacgacg cggcctgtcc
     4981 ggctgacgtg ctggcggcac gtgctgaggc tgcagcatcg gttgcatgag ttgccgcctc
     5041 gctggctgat tttttcgcgg ctgccgtatt ctgtgcaacc gcggaggcgt tacgtgacac
     5101 ctcttccacc atctgctcaa agcggcgcag tgcctccggt cggacatcat cctccgtcat
     5161 ggcaccgaga aaatcattca gcgtacctgg tctggaacct tcatagacgg taatggtccc
     5221 ggcatgtgaa ggcggaaaac cttcaaccag cagggtgacg ctgtactgac catgctcaac
     5281 atccatgctg taacgtccgg cttcatccgg attttcagag gccaccgtgt tcaccaccat
     5341 cgtgctgctg gttcgtctgg ccttcagcac aatggtgcag ttctgtactg gttttcctgt
     5401 gccatcttta agcacgccag aaatttttac tgtcatactt ttccaccaat aaaaaaagcc
     5461 cgcagcagtg acgccacggg cttcaggaca gtgtaacttt acgtttcctc aaacgcagtt
     5521 caccccataa ggtggatgaa cctgcgtatc ataacaatat ttacagaaga taaatcggcg
     5581 tctgttgtca gatacggtat ccgataccaa caataaatgc atccgttcgc cagtcaccac
     5641 taccggaacc ttcataagca aggtcaatgg tcacggattc ggtcgggtta aactgcacgc
     5701 cagcccccca cgccagagac gtgttgctgt ggcgaccgtc atcacttccg gtcagcacat
     5761 cgtgcgtttt ccccttgttg tcagttacgc ggagataatc cccggagaaa gtcgacacac
     5821 ggctgtaagc cacacccacc atcgcatacg cgctgaacca ttcattcacg cgtacagacg
     5881 gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag cgggtatcgc
     5941 tgtagtgcgt tttttgctca tcctcagcat tggcataact gaaggacgta atcagcccca
     6001 gcgcgtccgt aaactcataa cggtatttca cgttaatccc gttcagatta tcgctgccgg
     6061 gagcgttcgt acgggcatga agataccccg cgctcagtgt ggactgatgt tcagacgccc
     6121 atgcaggcgc accggatacg gacagacaga tggctgcgga caaaatggct gcacaaactt
     6181 tacgcataat tacctctcgc ttttctgcaa taaaaaaggc gccatttctg gcgcccgtat
     6241 atgggttata aaattcagct gatactgatg cctgcggtgg ctttcttcat caccacaacc
     6301 agcaaatcgc tgatacttgc tgtgggatac cagttattca ccagccatgc tgacaccgaa
     6361 aactccagcg tcatgtggcc gcgaccagct ggcatatcaa taacaccact gtaaatcagc
     6421 gtattatcca gcgcggtacg gttataaatt tcagcaccgt ttttccgtac tatcaggcgg
     6481 catgacgaat aaatatcgtt attctcccgc tcatgtttag cgccgctgaa tgccaccgcc
     6541 ggaataacaa tttgcctgtc aaaaggctga tcgtcataaa tcctgacggt aatggtccct
     6601 gatggccacc gctccggtgc ccgggagtcc cgcggaaaag ccttacccac tgttttgact
     6661 atatcgcctt caatctggtt ggctgacagt ttccccttaa tctgacagtt ctcattaatt
     6721 gtgacattgt tgagcgtccc ggcgttcgca ttcacactgc cactgatatc tgcattttta
     6781 gcggtcagtt ttccgtccgg tgtcagggaa aatgccggag gattaccgcc gctggtaatg
     6841 gtgggagccg tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca
     6901 acaaacatcg gctttgtgtt gccattcgca ggattaatca tcgcaatcct gtctgccgcc
     6961 agcagcacct gactctgcat tcctgctggc gtattctcaa taccggcacc gatacccgca
     7021 atataaaggc gtccgtcctt catctgttgc agcttcacag cccacatgct gttcagatta
     7081 ttatttgtat caacctgaac cttctgtatc tgctggattg ccgcactctg gtctttcagt
     7141 ttcttattga cagtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca
     7201 gccaggtcag gcgcaagctg accgttatca atctgcgtcc acagctcctg agccagatgt
     7261 gttttcccta tctctccttt gaaaaaatcc aagtaacctt ccgcatcatc gctcgcccga
     7321 ccgacagcct ccacgaatgc cgatttgcca acggtgttca cactgcgaac gtaaaaataa
     7381 taatcatggc ccggtttgat attgatactg gcggctatcc agtacagcgc cgtgccaaga
     7441 tagcgggcgc tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaac
     7501 tcaaactgta ccgtcgggtc ataaacggca agatgcggcg ttgcggttat ctgaaaatag
     7561 cccggcgtca gctcaatccg cgacggtgct gccggtgcgg caatccggaa ggtggtggtg
     7621 gcaggttcac cctgctggcc atagctgttt atcgcccgca ccgtcagggt gtattccccg
     7681 agcggcaggc cgctgaaacg gtgctccgtg tctgcggtga tggcggtggt caccagtctg
     7741 gcatccgttc ccttaccact ggtcaggcgc agactgaagc gcacaccctt caccacccgc
     7801 ggcgtgtccc atttcgcctg cgccagatac tggccgtcag gtgctgcact gccggtggga
     7861 tgacgctgtt cagggaacct gactgcggct caaagcgggc accgttatcc acgatggctt
     7921 ctttttccag tacgtgctgc accgccgtga tggcaaaggt gccgtccgtg ttttcccgga
     7981 cggagacaca gcggaacagg agacggcgca gtgacggcag ggagagtccc cacaccatca
     8041 ggcagggtac tgacctgtat ccggtccggc gcggggtgtt cggtgatgtc cacactcacc
     8101 ggcttaccgc tgccgttaat caggttcacc gtggcggcac cggtctccgg aagtgtcact
     8161 tcacggtcca gcgtcagagt gcgggtggca gcatcaatgg acaggacacg tccgccggtc
     8221 agggtcccgg catagtcgtt atcacagatt tcaatgatgt caccgggtgt gtgccgcagc
     8281 ccctgagacc cgagcgtgaa atccaccgtc tgcgtttcca gcagttcggt ctttatcacc
     8341 cacagtccgg cacggtgggc ctgaccgcgg ctggtacagc cgaacgcgtc catcttcagc
     8401 aggttgcgtc cgtagcgcag tatggcttcc gggtcttcca ccagttccgt ggaggtctgc
     8461 cagccgttct gcgggtcggt ataattcacc tccaccgccg tgtgccggtc cttcagggca
     8521 ctgaagctgt agcggaaacc cacgccgtta tcatccacca ccacatcgct gttggtgtac
     8581 ggccacacca catccgacgg gcggtcctga acaaacgtca gcgtctggcc gttccatacc
     8641 ggcatacagc gcatcgccga gcagaaatca ctgagcacgt cccatgcctt acgctgttgt
     8701 gccaggtacg cattgaaggt catccgcggc tcggtctccc cgaaactatc cgggaccgtc
     8761 tggtcgcagt actgcccgat ggcatacagc gcccacttgt ccacatccgc cgcccccaga
     8821 cgttttccca tgccgtagcg cgggtgagtc agcatgtccc acagacacca ggccggattg
     8881 ttgctgtatg ccggtttcag gctaccgtcc cagataccac tgtacgtgcg tttttccggg
     8941 tcatagtttg acggcacctg gatgatgcga ccgcggatat ggtagttcac cgtcatctgc
     9001 tggccgccga actgctccgc atccacctgc agccccacaa tggccgtgtt cgggtagcac
     9061 tgtttcacat cgatgatttc ggtgtatgac gaccacagcg tcttattctg cagctggtcc
     9121 gtggtgctgt ccgctgtctc ccggaccatc cggatgttaa aaggacgggg aggcagatta
     9181 tccagaatca ccgacgccag aaactgcgag gtggtcttgc cgttaatggt gacatccttt
     9241 tctgtcaccc agttaccgtt acgctgtaac tgaatcagca ggcggacgga tgtcgggttt
     9301 cggtcaccct ttgacgtggt ctgcaccagt gactgcaccc cgaaggtaac ccgcaggcgg
     9361 tcaatgttcg cggacgtaat ggtgcgcgtc accggttttg ccttcgtcac ttccacgccc
     9421 agtccggttt cagcaccgga ggactcaaag ccttccggtg gtgtctgctc ctgctccccg
     9481 gcgcgtcaga ccgccgtcac accgtgtatc acgggattac cgtccgtgtc cgtcagcggg
     9541 gttttgttca ccaggatact ctgcagtccc ttcaccggac cttctatcag tccctcacca
     9601 atcgcatcaa tcacgctcat catctgcgtg gatttgagat tatccttcgc ctcacgaggc
     9661 gtgtgtgcct taccgccacc ttttcccata cagccttccc ctgaataaat taaccgccac
     9721 ttgccattcc gtacagaagt cggatatcct tcgcccgaaa agcatgaaac acatttctgc
     9781 catgctaaag agaaaccccg gtatcagcag ataccggggt tttctttcat gcccaccgat
     9841 aatcctgttg gttaaaaccg gtaatggcat aaaaattctg aatatcttca cattttcaca
     9901 aactgactgt ggcgcgtata atttctctgc gttaattttt ttgtcgtgat ataagaataa
     9961 ttccttacac ttaatcttcg taactctccc gcagttcctg tccgcgatca ctgcgggatt
    10021 tttttattct ttttacccct gccgcccgat aaccacgacc tttccgcccc cgccttcatc
    10081 acgggtgctg atgtcctggg atatacggcg ggagccaacc agcatttccc cgtaaggcac
    10141 cggcatcggg ttcccctggg caatcatgtt atccagtgag gaaaagtacg tgttctgtct
    10201 gccgttatcc gttgcgcggt aatccggtgt ttttgccttc ggggccagca tctgggccac
    10261 accgcccaga atcatgctgg ctccaagtga aaacagcatc gtggtggcag aaaaaccacc
    10321 ggctgccagg gctgaacccc ataacgccat tgatgccccg gcagtgaaga aagagcccac
    10381 gatggctgcc gcccccagca caatctgcag tccacccttt ccggccccgg ccagtcgcgg
    10441 cacaatgtgg atgaccgttc cctcacccag ctgttcgtga agacgggcgt acaccgcctc
    10501 cggtgccgtg tcataaccgg caatacgtat ctggtaccag ccttcgttca tctgacggcg
    10561 aaagcccggc atctgcatcg acagggcgcg aatggcttcc gctgccgtgt tcacatacag
    10621 gctaaggcgg cggccaaatc gttgtaaatc cccgtgaagg cagatgcgtg ccagtggcgg
    10681 tgacgccaga ctgaatgcgt tcgtcgttgc catttttcgg aatacctctc ccgtttactc
    10741 agttgttcag gcagatggtg aagcagctca ccgttgccgc agtaaatggc ggcatggttc
    10801 ggtaccgaag caccaaagca gcacagcaga atatcgcccg cctgtgcaga ggacaggggc
    10861 acccggtaaa agccggtgac cgccatattg tccaggtaaa ggttctgacc gttgcgccac
    10921 cagtcatcct cgcgatgaaa atccggcatt tcagtccccg ccagatgata agcatcccgg
    10981 aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc gtcctgtcag atgtggcaca
    11041 cagcggaatt tgtgaatgtc accccggcag accaaccacc agggcagtgc gctttttatc
    11101 tgcaaccgcc ggtcagcctc gctcagccag ggcagcccac cgggatgact gtggaccagt
    11161 gccacaatct ccccctgcat ctctgcccgc agccagtctt ccggtgcgat acgaaaatac
    11221 gcctccggct ctgcggaaat attcacacaa gggatatacc gctccccctc cggcgtgctt
    11281 atcacgaagc cgcacgactc cgcaggcgca caccgccggg catgtgccag aatcgctgat
    11341 tcagtctgtg tcataaaccg ggatttactg cgaaagttta ttaatggaaa ggaaaccgcc
    11401 aaaattgccg acattcctgc gcagttcaca cccgcgcatg cacttgctgc atctgtcctt
    11461 acggatatcc gtggtggggt tgtcgaactc atccgccacc gcaggaccgt tatacccgca
    11521 ttcatctccc cggtaatccc acatacaggt gttcgccagc atgatgcgac cgggaaacag
    11581 cgccccgtcc gtctcggtcg gtgtagccag cacaaacgag gccgtcatgg ctgtcagctg
    11641 cgacatctgc tccaccaccc agcggtcact cagctcctgc tccgggtccg cctccggatt
    11701 gcccgcaacg aaattcaccg catccagaaa acgggcatac acccggcggc ggaccaccgt
    11761 ggcccccacc agactctgca ggtcttccgc catcccggtg accagaccga acagattgga
    11821 caccgtcagc gacggtctgg cactgctgcc ccggccgttc atctcaaagc cgctgccgtc
    11881 aatcgggtat gcctcatact tacgcccctg ccaggtgacc ggctcccctt tttcattcag
    11941 ctcattacag aaaaaatacc gctcaccacc ctgtaccgtc aggtcgattt cccagagtac
    12001 cacccgcggt gactgctctg acttaaccga ctcgttcaga ctttcttcgc gaatatcctg
    12061 catcagttca ccacctgctt aaactccgcg ctgaactcaa cgcgcaacat cccgacccgc
    12121 gcagaccacc cggcacaggt cacctttatc tgccggtatg catagggtgg cttccacaaa
    12181 aatgccttcc agccaccgtg ctctgccagg aacgcttcca gatgccgggc ctcctcccgg
    12241 gtcacggaaa gcgtcaccct gtatgttttc aggtcagcat tcagccctgc cgccatacgc
    12301 tgtgagtacc cgtcaccaaa acgcacttca cgcaccgatg gctgcgagtt cacctccata
    12361 tccggcttca ctttccagcg aaaggttttc atccaccgct ccctgataac ataccgccat
    12421 cacgcaactg cagccggagt tcatcctgtg cccccttgcg ggccatctca tacaccgctt
    12481 tcatcagctg cggccctgcc cgcccgttgg ggccgtcgtt ctgaatcacc acgtgattgt
    12541 tctgattaaa attaatgcct tccgcccgcc gcatctgcgc cggacttccg gcaccgccga
    12601 cataaccacc ttccgcatag ccccgcatca ggcggtacag attgccgaca ccaatccggc
    12661 tggtcgcctc cttcgtgaag acaaactccc cgcgatgaac aatccccgca ggttcatatt
    12721 taccccccgt ccccgtaaat cccccggtcg cgaaatggaa gttcgccgcc gcagcctgaa
    12781 tggccgtccc cgtggaggca gacgcaccac caccgaaagc accgccaatg gcgctgccga
    12841 tactcccgac tatccccacc atcgcctgct tcagaaaaat ctctgtcagc atggacagca
    12901 cagaacgggt gaaaccacgc cagttctgtt cgctgccgat cagcatcgct gccatattct
    12961 gtgcaatacc gtcaaaggtc tgcgtggccg cgtttttaac ctgcgaaaaa ctgtccgtcg
    13021 cactttccgc ccactcgccc cagccggact tcatcccggc catccagctt ccacgaagct
    13081 gctcctccgc agaccaggtg ttcttcagtg cagatgtggc cttcgccagc gcagccggat
    13141 tatcaccgta cacctcacga aggcgctgct cttccgactc ccgctgcgcc tgacggtcgg
    13201 tgagtccgcg ggcttttgcg ctgattgccg cctgcttcgc gctctgctgc tgttcaaacc
    13261 gcgccgcctg ctgtgccagc tcattcagcc gtttctggtg ttcaatcttg tcgcccagct
    13321 cagccagctg gcgtttgtac tccagcgttt ctttctcatg ggccagcagg gatttttcct
    13381 gctcagataa ctgccgtttc gtggcggcct ctttcaggac cacatactga ttttccgctt
    13441 tccataaatc acggcgctgc tggctgattt tttcattcgc accgctgtgc ttctccagcg
    13501 tcctgagctc agtttcaagc gccagcaggg ctgcatgcgc ctggtcttcc tggcgctcac
    13561 cggcagacac cttcacaccg gacggctttt tctgcgtcga ctcataatcc ttttttgccg
    13621 acgccatcag cgtgttgtaa tccgcctgca ggattttccc gtctttcagg gccttattca
    13681 gctcttcctg ccgggcggta tatttctcca gtggcgtcag caggcgctca tacgccttct
    13741 gcgcctctcc ggtatacttc agctgtgacg actcacgctc agccctgtcc cttgccgcca
    13801 gttcaccggc tttttccata tccgactgca gcgttgccgc tgccagaccc agacgggcat
    13861 tttcccggtc atcccatgcg ccctgaaggt tggcccggaa agaggaggtt ttaccgcggc
    13921 gctggctccg actctggtac cactgccatt ttttatccgc ctcatcaaat gccttctgcg
    13981 cactggcgag catatccgct gaggattccg gacgaccgat atccagaatg gcatcccaca
    14041 tcgatttgaa tgccttccct gttttatccg cccaggtctc cagtgttccc atgttttctt
    14101 tcaggcgacg ggtctgctca tcaaagcctt tcgtggcgat atcgttcgcc gcctgcaatg
    14161 ccccggcctc gtctccggaa cgctgcagct gtgcaacata cgcaatctgc tctgccgtca
    14221 cgttacggaa ctggcgcgcc atcgccatca gtcccgacgt cgggtcagtg gtcagcttcc
    14281 cgaaggcttc agcgacttta tccacctcca caccggatgc agacgcaaaa cgcgcgacac
    14341 tctggttgat ggcatcaaac tgttcaccac cacgcacacc ggcattcacc agggctgcca
    14401 gtgactctct cgcctggtta aacgtcagcc ctgctgcctg cccggctctt gagagagtca
    14461 gcatacgatc ggcagtcagt ccggactgat taccggaaag aaccagggtt ttattaaacg
    14521 ctgaaagcgt ggaatctccc tggtaccagg cgtacaccag tgcacctgtc gccaccgcca
    14581 gcgaggtgac cccgaccatc ggcagggtga tcgcatcggc aagccccctg aacatgggga
    14641 tcatcccgcc gaaggagtcc ttcacctgac cgccctgttg cagcaggatc agccagggat
    14701 tctgaccacc ggcaagctgc gtggcgatat ccgtaaactg tgcgggcagg gttcgcatgg
    14761 ccgctttata ctgcccgacg gaaatcccgg ctttttgtgc agccagcgcc tggcggctca
    14821 ggccctgttc aacagcactg gcggtttttc tggcgtcggt atccagacct gaaaaatgac
    14881 gccttacccg gctcatctgc tcatcgaaac ggacagcatc cagactcagg tcaataacaa
    14941 gatcaccaac cggctgggac atatctcaca cctcccggaa tccccgctga agccatcatt
    15001 aatgcggcat catccaccat gacatccgcc acatccgcag acgataaaat atcgcgccct
    15061 ccgtccccac cgaaccggac gcctccggca agtcctgccg ctttctgcat cagcattttg
    15121 tcctcatccg gcctctccac ctgctcttcc tcatgccggg ggacaagcag actgaaatca
    15181 gagggatgca tatccggatc gcaaaaaaac aggctgagta cagcgtacgt cagcccggaa
    15241 aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc ggtaaaaacg gcgccagtcg
    15301 gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct ccccatctca
    15361 cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga ctttcccgca gaaaaatcat
    15421 catcagtcag cgtgttattt ttcgccactt cagtaatatc agtatccgga cgaacagctt
    15481 cgatcatccc ggacaggcac aacaccacgt cttccgcccg ggcaatggca tcggcaggcc
    15541 aggtggtgag cacttcctgc tctatcttca tcacggcctc attcattgac ggtgactgcg
    15601 ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgcgccg gttctgacga
    15661 gatcttccac gcttacctgc aggttgccgc tggattctgc ctgttctgca cgccgtttca
    15721 ggagggcaag atgctcgata cgctgcagcg cagacaattc ggaaagcgtg acagacacac
    15781 cgttatattc aaattgttct gttttcagaa acatgtatta cctccgttta ccctgcagcg
    15841 cccgcttcag taacggtgac ttcagccact gcggcgaact gaccatttcc gctcaccaca
    15901 gggatctgca ccttacctgt cgccacgccg tttaccgtaa ttgtcatatc tttcacacta
    15961 atggtggctt tcgacggatc ggcggaaacc gctctgaacg tcttgtcggt tgcactttcc
    16021 ggctcaaaag aaaccgtcag ggtggttgtt ttcccttttg ccaccgtacc ggatgtcggc
    16081 gtcaccttaa tcgcactgac cggcgtaatt ttgctgcgtt cttccgctac agaaggttta
    16141 cccacgttag tgactttcac cgtgcgggtg atcacttctt tcgccgtcac ggccttaccg
    16201 atactgctga cccagccacg aaacacatcc accgtgccat tcggaaaacg gattttatag
    16261 gcccggacat cgccgctttc aaaccagcct ataagccctt tctggccttc ctctcccggt
    16321 ttccaggcca gcgtaaaact ggtatcacct gcagatttct gcccctgccc ggtcgcgctc
    16381 cagtccgcgt cttcatcatc caggtagtta tcatcatagg attcagccgt catctcgccc
    16441 ggcgtcagat ccttcacctt agccagtcgc tgccagtcat cgtctgacaa cgggtttgca
    16501 taagcatcag ccttgccgtt gtaaacccac agagtggtac cggcaccttt taccggctcc
    16561 aggggatttg gtgttgccat atcgtcctca catctcgtat gtaatggaat aagtcagatc
    16621 cgcagagctc cataacgcca tatcgtcatc acgacgatac tcatagccct gcgtaaccat
    16681 cgtggtaatc agtcctgcca gtgcagggat cgcagtcatc gccggataaa tccggctttc
    16741 catccactga tcgagctccg aatcaggtac ctgtgccggt aaaaacacct caatatgcag
    16801 tgtggcccgc caggtatctg catccagctc ttcaccggta tactctgcat ccgtcagata
    16861 aaccgcgatc gcaggaaaat cctcttcgtc aaaaacaacg gggcgaccat caaacagcgt
    16921 cgccccgtgt tcatgctgct cgagtgcatc cagcactgcg gcacgaatgt cagtgtgttt
    16981 catcgtttta tcgcaatcct cagttgttgt ttcagcgcgt atgccagttc tttaggcagg
    17041 cgttcacgcc ggatacggtc aacattctca tcaaatgcct gtttcagtgg ggccgccatc
    17101 gggattttca ccacctgaat gggaaggcga ttacgctttt tccttccctt gtcgtcattg
    17161 ccctcctcat atctggcctg gggaagacgt tgcataacat gccagcgccc attatttaat
    17221 cgctggataa atgcccgctg ataacgatgc tgaccggctt tgagtatgct gttcggacga
    17281 cgccccagca ttctgatccc cagcttaatc acagggagat caccgcggtt aacgataatt
    17341 ctggcattcg gatttctgac cgtcgcccgt ttcagtctgg accgttcctt taccagtttc
    17401 cgtctcaccc tggtttcccg ggcaacctgt gacgaagact gattaatcgc cgttgtggcc
    17461 acgcggttaa tggtcattgc agaagcagcc ggaatggcgt ttttacgaac ccggttcaga
    17521 ttgtcaatcg cctgatcaag cccttttatc gccataattc accctgcgtt tatcgtcgcc
    17581 ggttaactgc gggtggttgc ccacggttga gccagagata acagctgccc ccgtcatccg
    17641 g
//
LOCUS       DAZOYS010000080        16982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOYS010000080 DAZOYS010000000
VERSION     DAZOYS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16982
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.080"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUN9_002291"
     CDS             <1..266
                     /locus_tag="ACHUN9_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0695673.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUN9_002292"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUN9_002292"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0695674.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUN9_002293"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUN9_002293"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0695675.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUN9_002294"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUN9_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0695676.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUN9_002295"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUN9_002295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0695677.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHUN9_002296"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHUN9_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHUN9_002297"
     CDS             4828..6600
                     /locus_tag="ACHUN9_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0695678.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHUN9_002298"
     CDS             6593..8374
                     /locus_tag="ACHUN9_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0695679.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUN9_002299"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUN9_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0695680.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUN9_002300"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUN9_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0695681.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUN9_002301"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUN9_002301"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0695682.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHUN9_002302"
     CDS             11336..11908
                     /locus_tag="ACHUN9_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0695683.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHUN9_002303"
     CDS             complement(11939..12250)
                     /locus_tag="ACHUN9_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0695684.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHUN9_002304"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUN9_002304"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHUN9_002305"
     CDS             12629..12982
                     /locus_tag="ACHUN9_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0695685.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUN9_002306"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUN9_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHUN9_002307"
     CDS             complement(14736..15206)
                     /locus_tag="ACHUN9_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0695686.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUN9_002308"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUN9_002308"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0695687.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHUN9_002309"
     CDS             complement(16044..16262)
                     /locus_tag="ACHUN9_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0695688.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUN9_002310"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUN9_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0695689.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4050 a         4117 c         4554 g         4261 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aa
//
LOCUS       DAZOYS010000081        16884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOYS010000081 DAZOYS010000000
VERSION     DAZOYS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.081"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..151
                     /locus_tag="ACHUN9_002311"
     CDS             <1..151
                     /locus_tag="ACHUN9_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0695690.1"
                     /translation="PTFHEIRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYLDEREK
                     TYLLL"
     gene            complement(544..885)
                     /locus_tag="ACHUN9_002312"
     CDS             complement(544..885)
                     /locus_tag="ACHUN9_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0695691.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(898..1770)
                     /gene="copD"
                     /locus_tag="ACHUN9_002313"
     CDS             complement(898..1770)
                     /gene="copD"
                     /locus_tag="ACHUN9_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0695692.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1774..2148)
                     /gene="yobA"
                     /locus_tag="ACHUN9_002314"
     CDS             complement(1774..2148)
                     /gene="yobA"
                     /locus_tag="ACHUN9_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0695693.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2287..2517
                     /gene="holE"
                     /locus_tag="ACHUN9_002315"
     CDS             2287..2517
                     /gene="holE"
                     /locus_tag="ACHUN9_002315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0695694.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2619..3275
                     /gene="yobB"
                     /locus_tag="ACHUN9_002316"
     CDS             2619..3275
                     /gene="yobB"
                     /locus_tag="ACHUN9_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0695695.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            3299..3961
                     /gene="exoX"
                     /locus_tag="ACHUN9_002317"
     CDS             3299..3961
                     /gene="exoX"
                     /locus_tag="ACHUN9_002317"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0695696.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3958..6018)
                     /gene="ptrB"
                     /locus_tag="ACHUN9_002318"
     CDS             complement(3958..6018)
                     /gene="ptrB"
                     /locus_tag="ACHUN9_002318"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0695697.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(6227..6886)
                     /locus_tag="ACHUN9_002319"
     CDS             complement(6227..6886)
                     /locus_tag="ACHUN9_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0695698.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(7213..7569)
                     /gene="yebF"
                     /locus_tag="ACHUN9_002320"
     CDS             complement(7213..7569)
                     /gene="yebF"
                     /locus_tag="ACHUN9_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0695699.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(7636..7926)
                     /gene="yebG"
                     /locus_tag="ACHUN9_002321"
     CDS             complement(7636..7926)
                     /gene="yebG"
                     /locus_tag="ACHUN9_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0695700.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            8060..9238
                     /gene="purT"
                     /locus_tag="ACHUN9_002322"
     CDS             8060..9238
                     /gene="purT"
                     /locus_tag="ACHUN9_002322"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0695701.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(9294..9935)
                     /gene="kdgA"
                     /locus_tag="ACHUN9_002323"
                     /gene_synonym="eda"
     CDS             complement(9294..9935)
                     /gene="kdgA"
                     /locus_tag="ACHUN9_002323"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0695702.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9972..11783)
                     /gene="edd"
                     /locus_tag="ACHUN9_002324"
     CDS             complement(9972..11783)
                     /gene="edd"
                     /locus_tag="ACHUN9_002324"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0695703.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12018..13493)
                     /gene="zwf"
                     /locus_tag="ACHUN9_002325"
     CDS             complement(12018..13493)
                     /gene="zwf"
                     /locus_tag="ACHUN9_002325"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0695704.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13831..14700
                     /gene="hexR"
                     /locus_tag="ACHUN9_002326"
                     /gene_synonym="yebK"
     CDS             13831..14700
                     /gene="hexR"
                     /locus_tag="ACHUN9_002326"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0695705.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14828..16270
                     /gene="pyk"
                     /locus_tag="ACHUN9_002327"
     CDS             14828..16270
                     /gene="pyk"
                     /locus_tag="ACHUN9_002327"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0695706.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(16330..>16884)
                     /locus_tag="ACHUN9_002328"
     CDS             complement(16330..>16884)
                     /locus_tag="ACHUN9_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0695707.1"
                     /translation="SYLKIIEQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLE
                     LDVTNIVASDEMYCVYAIDPDSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIG
                     AGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLA
                     SYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         4213 a         4297 c         4126 g         4248 t
ORIGIN      
        1 accaactttt cacgagatca gaagcctggc tggacgattg tacaaagaaa catgtggaga
       61 agaatttgct cagcgtctac ttggccacac atcggagaag acaacaaaaa tgtatcttga
      121 tgagagagaa aaaacgtact tactgctctg attttaacgt aaatggattg ttaaatgtat
      181 tttggttgtg atataaccaa aaagaccgga atacagaaat tcgagtaaat ttcggggaat
      241 ttcgggggag acatttgcaa ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg
      301 attcctgtat tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc
      361 attaatgcag gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag
      421 tttgcgtgca aaatggtcaa taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg
      481 cccgttctgg tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg
      541 taattaacag agtttttcag ctcgttctat aaacggtgcc agactcattt tttcgccggg
      601 attgttagga tcatcaatct gaatcaccga aatgggttgg gcattggtct tcccactggc
      661 aacttccttt tgtgcgatat cgtttaaagg atactgcacg agggtactcg gattaataac
      721 atacaaagca ttacccggtc ggcaagtcag catcacctct tcgcgattaa acgcccattt
      781 gtctttaccc acttcaaaac gactgacggt aatcacctgc ggtgcagcca gcgccgctgc
      841 agaactggtg agtaacagaa acgccagaat actttttttc atcattttcg taatccatca
      901 aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca
      961 ttcgagttta gtcatccaca caaaccaggg tgccgcacga tcttcctcct gcctcatgcg
     1021 cggtacaaga acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc
     1081 tttgagcaat aagagttggc cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa
     1141 caatgcatta agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga
     1201 aaaacacatc agcgcctgaa tagcctggtg tcgccaacgg cctttgatga gttgcataca
     1261 ccagagcacc ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg
     1321 attagtctga tggattttcg ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc
     1381 cagcagaata aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg
     1441 cataagggcg acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc
     1501 aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa aatacatctg tccatcccgt
     1561 tcccatcaac ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac tccagacggc
     1621 agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc
     1681 cagccaggca ccgtacattg cgaagccaaa aaccagcatc agcgaggtga aatggataaa
     1741 tcgcagcgcg atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc
     1801 tttcgtttta tgcccgtcca ccgaaacaac atgccagtca acggtgtagg ttccgggttt
     1861 cagcgaatcg gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg
     1921 taatgtttta atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc
     1981 cacaccttcc gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc
     2041 cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt
     2101 ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt gaagccatat tatctattcc
     2161 tttttgtaat aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc
     2221 aattccggct tgccatcctg gctcactctt agtaactttt gcccgcgaat gatgaggaga
     2281 ttaagaatgc tgaagaatct ggctaaactg gatcaaacag aaatggataa agtgaatgtc
     2341 gatttggcgg cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa
     2401 gcggttgaac gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc
     2461 caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac
     2521 ttatacaggg ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg
     2581 cacccataat ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag
     2641 cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca agtagctcat catctggagt
     2701 ttgtgagggc cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac
     2761 tggggtgtga ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc
     2821 cgctttgtta tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat
     2881 ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga
     2941 tctaccatca gagtcatggt gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg
     3001 atgaacaacc ggaaggtatg gatatggacc cgacctgttc actgtttacg acagggcaat
     3061 gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt
     3121 actcgattgc tgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg
     3181 gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac
     3241 agggttggca aggcgatatc attccattac gctaggcttt ttcggcctgg agtatgccat
     3301 gttgcgcatt atcgatacag aaacctgcgg tttgcaggga gggatcgttg agattgcctc
     3361 tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg
     3421 tcctattagt ccacaagcga tggcgattca tcgcatcacc gaagccatgg tcgccgataa
     3481 accgtggatt gaagatgtga tcccacacta ttacggcagt gaatggtatg tcgcgcacaa
     3541 cgccagcttt gaccgccgcg tactgcctga gatgcccggt gagtggattt gcaccatgaa
     3601 actggcccgt cgcttgtggc caggaatcaa gtacagcaat atggcgttat ataaaacacg
     3661 gaagctcaat gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt atgactgtta
     3721 tatcaccgcc gcgttgctta tcgatattat gaacacctcc ggctggacgg cagaacagat
     3781 ggccgatatc accggacgtc cgtcgttgat gacgaccttc acctttggca aataccgtgg
     3841 caaagcggtt tccgacgttg ccgaacgcga tccgggctat ctgcgctggt tatttaataa
     3901 cctggacagc atgagcccgg agctgcgttt aacactgaaa cattatctgg aaaatactta
     3961 atccgcaggc gtagcgggta atgttccctg cgccagcgcg accagaaaag catattccat
     4021 cgctacgcct tcgtacgatt taaagcggcc agatttaccg ccatggcctg agtccatgtc
     4081 ggtacagagc aataaaagat ggttatcggt tttcagctcg cgcaatttag cgacccattt
     4141 tgccggttcc caatattgca cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg
     4201 ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt ttcatgtact cgtaatattg
     4261 cggatcctgc gggttacccc actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc
     4321 aagcatcgtt gttacaacat caacaaacgg tacctgggcg ataacgccgt ggaataattc
     4381 cgggcgttga ttaattgcaa cgcccatcaa catgcccccc gcactcccgc ccatcgcata
     4441 acaaagcgaa ggagagccat agcccagttt taacaatgca tcgcaggcat caagataatc
     4501 attaaacgta tttttcttct tcagaaattt tccgtcttcg taccattgtt gccccagctc
     4561 accaccgccg cgaacatgga caatggcgta gacaaagcca cgatctaaca aactcaagcg
     4621 gctaaaactg aaatcggcat caatacttgc gccgtaagaa ccatagccat acaccagcaa
     4681 cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc aacgaaaccg gaacttcgac
     4741 gccatcacgg gcgactatcc acaggtgttc actgcggtaa ttcgccgcat caaaaccagg
     4801 aacttccgtt tgttttaata cacgacgctc atcggtatcc atatccagtt caaacaaagt
     4861 gtctggtgtg gtcatggaag aataaccata acgcaatcgc gcggtttcag gttctggatt
     4921 gtaggcaatc caggtcacat aggctggatc atcaaaggca ataccaatga cttcccgggt
     4981 cttgcggtta atttggcgca aactggttaa cccgcgctga cgctcttcaa ccaccagcca
     5041 gtcggtaaac agcgtaaacc cttccagcat gatgttttcg catggcggaa ttaactcttc
     5101 ccactgttgc tcatcacgca tacgggtacg gtataagcca aagtttttgc cgtggcggtt
     5161 ggaacgcaga taaaaacgat gctggtagtg atcaaggctg tattcgtgat ctttgcggcg
     5221 cggcagaaaa acaaacggct cggcatcggc catttccgcg tccagcaggc gaacttcact
     5281 ggtggtggcg ctggccaaat gaatgactac atagtgcttc gaggtcgttt tatgcaggct
     5341 gacgtaatag gtatcgtctt tttcttcgta gatcagttta tcttgcgatg ctggcgtacc
     5401 gatggcgtga cgccagacct gataaggcag cagcgtcacc ggatgcttgc gaacatagta
     5461 gaaagtccag gagtcatttg cccagacaaa gctgggttca acgttatcca gcagttccgg
     5521 gtaccagtta ccagtttcca gattacgaaa acgaatgccg tactggcgtc gggaaagaaa
     5581 atcttctgcc agcgccataa tggtgttatc gggcgtaatc gccattccgc ccatcgaata
     5641 aaactcacta tgagctgcgc gcttattggc atcgagcaat atttcccact catcccactc
     5701 ttcactgaat gccgattgac gctggtagat agcatattca cagcctggtt cataaatatg
     5761 ccgatagcgg tagccatttt tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc
     5821 gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt gaggccatca cccgatgacc
     5881 gtaactattt tcttgttgca ggtagtccag gacttctggc tgagaacgcg tatcatcccg
     5941 cagccagtag taattatcga tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg
     6001 ggcggctttt ggtagcatgt tattgttctt tctggttgaa acatcttata agggtggcaa
     6061 aactcaccgg ggatgcaagc gaaacagggg agtcattgct tagatgatga caggtaatgg
     6121 cacggatatc gaatgttatg cagacagaga aactcagcct gttcaaatgg ctgtgcggtt
     6181 ctggatagcc cgaaatagtc aacttcaggc tatccagaga gcggaattat tccgccaaag
     6241 tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg aacatcctgg gggattttca
     6301 gcgcgtcacc cagtgcattc aggtaactgc gttccataaa atggtcaata tcaatagccg
     6361 cgcaactcag gaaatagatt tccagcgcct cttcttcatt gcggaccccg gtagccaggc
     6421 gttgtggatc cagcggttgt tcgattgcct gctcaatgag tacacgcccc tgctcttcca
     6481 cgccggcttc acgcaattgc tggtcgattg ccgcacgttc tttggcatca atatgaccat
     6541 cacttttagc ggcaaagacc agcgcaagga tcaaacgttc tgtacgctca tccagcggcg
     6601 tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt
     6661 tattccacag caccgtaccc gctaccgctc cgccgccaac cagtaacgcg ttggtgccat
     6721 atttcgtaag aagtttacgt gctgatttat ttgcgaccag cagcccagcc agtccgccta
     6781 atgcgcctgg cactaacagt ttgcccaatc cctgatccgc agacgaggag gtagaagaac
     6841 tgctttgccc aagcagggat tgcagttgat ttaaccagtt agccatattt gctcctcaat
     6901 aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta tatcgagaca
     6961 aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc gccaggtaag
     7021 gtatttactg tatatcaggt gataagggta ttttaggtga aagtagtttg tcgttttgag
     7081 tggcattgtt tgatgctgat tgcccgatgc gacgctgttg cgtcttctca ggcctacgat
     7141 tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat
     7201 tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt ccacgctgac
     7261 ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc atttatcatc
     7321 tttaccctga atgtcctgca aactgaccca agccacggga tcggcctgac cgacaatttt
     7381 ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc
     7441 aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc tggtttcatt
     7501 attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa acgcccctct
     7561 ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta caaattgtca
     7621 ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac
     7681 gacctgtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc
     7741 tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaatttgt
     7801 cagccaggtg tcaagcagat ccgccgtatc aagcattttg tcataagcat cggcttcctt
     7861 tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac
     7921 cgccatttgc agcctctcat aataactgtg attttataca gtatatttct tttcggttga
     7981 gaaatcaaca tcagcaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat
     8041 taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg
     8101 cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct
     8161 cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca
     8221 tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga
     8281 aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact
     8341 tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg
     8401 cgagggtatc cgtcgcctgg cggcagaaga gctgcagctg cccacttcca cttatcgttt
     8461 tgccgatagc gaaagccttt tccgcgaggc ggttgctgac attggctatc cctgcattgt
     8521 aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgttctg cagagcaact
     8581 tgctcaggca tggaagtacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt
     8641 tgaaggcgtc gttaagtttg acttcgaaat taccttgcta accgtcagcg cggtggatgg
     8701 cgtccatttc tgtgcaccgg taggtcatcg ccaggaagat ggcgactacc gtgaatcctg
     8761 gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt
     8821 ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga
     8881 ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc
     8941 tcaagatctt tcagagtttg ccctgcatgt acgtgccttc ctcggacttc cggttggcgg
     9001 gatccgtcag tatggtcctg cagcttctgc cgttattctg ccacaactga ccagtcagaa
     9061 tgtcacgttt gataatgtgc agaatgccgt aggcgcagat ttgcagattc gtttatttgg
     9121 taagccggaa attgatggca gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt
     9181 tgttgacgcc attgaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac
     9241 ccggcaaaaa aacgctacaa aaatgcccga tcgtagatcg ggcgttttga cttttacagc
     9301 ttagcgcctt ctacagcttc acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc
     9361 agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg
     9421 tagtcacggt agttagccgg agaaatacca cccgtcgggc agaaacggac ctgggagaac
     9481 ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg
     9541 aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga
     9601 attagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta
     9661 attgcgaact gtgcacccgc ttcagtgact tctgccagct gctgtggatt cagcaccgta
     9721 ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca
     9781 cactcggtac gcagagtcac ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc
     9841 atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc
     9901 aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca
     9961 aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca
    10021 cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg
    10081 tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt
    10141 ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct
    10201 tctggtgtta cgtggatagc tgacggcact ttacctgaag cgccggagag tcgtccatcg
    10261 gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta
    10321 tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg
    10381 cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc
    10441 gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg
    10501 cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa
    10561 gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtcctg ttcaccatta
    10621 ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct
    10681 tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc
    10741 tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca
    10801 tcagaaaggt cagagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc
    10861 accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac gataccgttc
    10921 accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc attaccggtc
    10981 atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga
    11041 tgaacaaaag aagagcctgg caactgcatc cccataaatt ccaccaccat ctggttggtg
    11101 ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc
    11161 agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct
    11221 ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca
    11281 agcgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga
    11341 gcaccatcaa acatgttatg ggacagcccc accgccgcag acatcgctat cacttcgcgg
    11401 cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga
    11461 acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct
    11521 ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg
    11581 atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa
    11641 ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt
    11701 tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt
    11761 acgcataaca attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat
    11821 tcacaacgcg tttcattcag aggatttatg agtgaaacgc ctgtaaccgg agctcatagg
    11881 gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct
    11941 gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata
    12001 aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc
    12061 gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc
    12121 atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac ttcatcacga
    12181 cgtacaaaca gtgcctgaat accacgcatg gtttccagca gcagacgttc ataggcatcc
    12241 gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg
    12301 ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca
    12361 ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgactc tttaaacaga
    12421 ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta
    12481 ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc
    12541 gccacgaagg tttctgtatt gctgctcttg ttcgcgccct cttcttccag atatcccggc
    12601 acttttttgc cctgggcgaa gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg
    12661 ttggagcggt cgatgcggcg cagggacttc agtactttca ctttttcatc gcggatgctg
    12721 tctgcgctca ggtcagatgg cggagacatc gcaatcatgc agagaatttg tagcaggtgg
    12781 ttctgaatca tgtcacgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc
    12841 cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc
    12901 acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga
    12961 tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt
    13021 tcctgcgagg tcgccagcga cgtccccagc ggtttctcca tgactacgcg tgccggttta
    13081 gcattcagtt ttgcctcgcc aagccctttg caaattgcgc caaaagtgct gggcggcatg
    13141 gcaaagtagt taatggtgat acgatttttt tgatccagca tcgcgccgag acggttgaat
    13201 gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat
    13261 aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac aactttggta
    13321 tatgccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc
    13381 tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct
    13441 ttcgcgccga aaatgaccag gtcacaggcc tgggttgttt gcgttaccgc catgtcattc
    13501 tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc
    13561 actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg
    13621 ggaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc attcggtaaa
    13681 aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt
    13741 aacacggcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt cagtgcggaa
    13801 atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat ccagtctcag
    13861 ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat
    13921 aacgcgatcc attcgagtat tgctgctatg gcactggaag ccaatgttag cgaaccgacg
    13981 gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg
    14041 gcacagagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga agatgacagc
    14101 gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga tcatgtccgt
    14161 cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa
    14221 atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc
    14281 tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg catgagttgt
    14341 atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat
    14401 ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct
    14461 gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat
    14521 actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg
    14581 gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag
    14641 gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa
    14701 aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc
    14761 gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc agtcaacgga
    14821 gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac
    14881 agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa
    14941 cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc
    15001 cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt
    15061 atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc
    15121 caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc
    15181 tgctgacgtc gtgcctggtg acatcctgct gctggacgat ggtcgcgtcc agttaaaagt
    15241 actggaagtt cagggcatga aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa
    15301 caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga
    15361 caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc
    15421 acgctgtggc gaagatctga actatgcccg tcgcctggca cgcgatgcag gatgtgatgc
    15481 gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga
    15541 catcatcctc gcctctgacg tggtaatggt tgcacgtggc gacctcggtg tggaaattgg
    15601 cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg
    15661 agcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg
    15721 tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc
    15781 tgcagaaact gccgcagggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg
    15841 cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt
    15901 cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg
    15961 cgttacggcg attattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat
    16021 cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc
    16081 tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc
    16141 cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg acctggtgat
    16201 tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac
    16261 ggtagagtaa tctcacctgc cccggccgct gatggccggg gtatttagca acacataaat
    16321 taggactact tattcaatcc aatcataatc acagccccct gtaaaatgaa tacagggata
    16381 gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct ctgcaacact
    16441 atgtccaata actgccatcg gtaagccact atctttggag tattgtgtct gcacccatgt
    16501 tttaattgaa tcgttaatcg taatataata atcagcctga tattccatat attgtattgg
    16561 atcacaaaaa cagcctgccc caatgccaac agttctgact actctggctt tatctccata
    16621 aaattgaatg ccaggaagat tatatatttt caaatttttg gcaaaacgtt tggtaatgtt
    16681 aatggcactg tcagggtcta tagcatatac acagtacatc tcatcagaag caacaatatt
    16741 tgtaacatcc agttctaact gctgagccaa agccttagac attccgaacg taggctctcg
    16801 atccatgaca tcatggcaac gaatgattac caaattattt ttatgcaaaa actccttttt
    16861 ttgctcaatt atttttaaat aact
//
LOCUS       DAZOYS010000082        16621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOYS010000082 DAZOYS010000000
VERSION     DAZOYS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.082"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..425
                     /locus_tag="ACHUN9_002329"
     CDS             <1..425
                     /locus_tag="ACHUN9_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR0695708.1"
                     /translation="SGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLR
                     NHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKN
                     TYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
     gene            complement(481..3336)
                     /gene="valS"
                     /locus_tag="ACHUN9_002330"
     CDS             complement(481..3336)
                     /gene="valS"
                     /locus_tag="ACHUN9_002330"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0695709.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3336..3779)
                     /gene="holC"
                     /locus_tag="ACHUN9_002331"
     CDS             complement(3336..3779)
                     /gene="holC"
                     /locus_tag="ACHUN9_002331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0695710.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3913..5424)
                     /gene="pepA"
                     /locus_tag="ACHUN9_002332"
     CDS             complement(3913..5424)
                     /gene="pepA"
                     /locus_tag="ACHUN9_002332"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0695711.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5578..5628
                     /gene="ytgA"
                     /locus_tag="ACHUN9_002333"
     CDS             5578..5628
                     /gene="ytgA"
                     /locus_tag="ACHUN9_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0695712.1"
                     /translation="MSIIILATISTTQENK"
     gene            5691..6791
                     /gene="lptF"
                     /locus_tag="ACHUN9_002334"
     CDS             5691..6791
                     /gene="lptF"
                     /locus_tag="ACHUN9_002334"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0695713.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6791..7873
                     /gene="lptG"
                     /locus_tag="ACHUN9_002335"
     CDS             6791..7873
                     /gene="lptG"
                     /locus_tag="ACHUN9_002335"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0695714.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7992..9494)
                     /locus_tag="ACHUN9_002336"
     CDS             complement(7992..9494)
                     /locus_tag="ACHUN9_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0695715.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(9572..10570)
                     /gene="idnR"
                     /locus_tag="ACHUN9_002337"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(9572..10570)
                     /gene="idnR"
                     /locus_tag="ACHUN9_002337"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0695716.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(10637..11956)
                     /gene="idnT"
                     /locus_tag="ACHUN9_002338"
     CDS             complement(10637..11956)
                     /gene="idnT"
                     /locus_tag="ACHUN9_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0695717.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12033..12797)
                     /gene="idnO"
                     /locus_tag="ACHUN9_002339"
     CDS             complement(12033..12797)
                     /gene="idnO"
                     /locus_tag="ACHUN9_002339"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0695718.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(12821..13852)
                     /gene="idnD"
                     /locus_tag="ACHUN9_002340"
     CDS             complement(12821..13852)
                     /gene="idnD"
                     /locus_tag="ACHUN9_002340"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0695719.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14069..14632
                     /gene="idnK"
                     /locus_tag="ACHUN9_002341"
     CDS             14069..14632
                     /gene="idnK"
                     /locus_tag="ACHUN9_002341"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0695720.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            complement(14636..15655)
                     /gene="ahr"
                     /locus_tag="ACHUN9_002342"
     CDS             complement(14636..15655)
                     /gene="ahr"
                     /locus_tag="ACHUN9_002342"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0695721.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            15851..15935
                     /locus_tag="ACHUN9_002343"
     tRNA            15851..15935
                     /locus_tag="ACHUN9_002343"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15885..15887,aa:Leu,seq:caa)
     gene            16121..>16621
                     /locus_tag="ACHUN9_002344"
     CDS             16121..>16621
                     /locus_tag="ACHUN9_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0695722.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
BASE COUNT         3972 a         4336 c         4169 g         4144 t
ORIGIN      
        1 gttctgggta tgcaaatgat gatattgaga atttacagca atttatggaa atgcaacgta
       61 aaatgataat cgcgcagtta atctattatt ttgggattgc tgttagcacc agttatttaa
      121 cggtgtcgtt gcgaaatcat tttatgagca acctgtcact gaatgatggg cgtattcgtt
      181 ttcgctcaac tttaacatac cacggtatgc tttatcgcat gtgtgcgttg gtggtgatat
      241 ccgggattac gggcggtctg gcttatccac tgctgaaaat atggatgatt gactggcagg
      301 caaaaaatac gtatttgctg ggcgatttgg atgaccttcc tttaatcaat aaagaagaac
      361 aaccagataa aggattctta gccagtattt cacggggaat tatgccttct ttaccatttc
      421 tgtaagagat aaaaaaggcc ggagcatgct ccggccttcg ttttcatcac tgtgttttga
      481 ttacagcgcg gcgataacag cctgctgttc aatcagtttc gctttcgctt ccgcatagcc
      541 ttccagcttc tcacgctctt tcgcgatgac cgcttccggt gcgcgggcga caaagccttc
      601 attcgccagc ttgttctcga tacggctgat ttcgccttcg atcttcgcca cttctttcgc
      661 cagacgcgcc agctcatctt ctttgttgat gaggccagcc atcgggatca gcagctctgc
      721 gccgtcgatg atcttcgtaa cggaaaccgg acctttgtca tcggcaggca gcacggtgat
      781 gctttccaga cgcgccaggg tttgcaggaa gccacggttt tcatttacgc gacgttctgc
      841 atccgcgctg caaccacgca gcagcagctc cagcggtttg cccggcgcga tattcatttc
      901 tgcacggatg ttacgtaccg caacgatcgc ctgtttcagc cattcggtgt cggccagtgc
      961 ggcttcatca acctgagatg catcgtactg cgggaacggc tgcagcatga tggtgtcggc
     1021 agtgataccg caaagtactt tcacacgctg ccagatggtt tcggtgatga acggaatgat
     1081 cggatgcgcg aggcgcagca gaccttccag tacggtcacc agcgtatggc gagtaccgcg
     1141 cagttcggct tcggtgccac cgttcattac cggcttagtc agctccagat accagtcaca
     1201 gaactggttc caggtgaact catacagaat gcctgcagcg atatcgaagc ggaagctgtc
     1261 cagcgcttcg cggtacgctt tgatggtctg gttgaactct gccagaatcc agcggtccgc
     1321 cagcgacagc gtcatttcac caccgttgaa gccgcaatcc tgaccttccg tgttcatcag
     1381 cacaaagcgg ctggcgttcc acagcttgtt acagaagtta cggtaacctt ccaggcgctt
     1441 catatcccag ttgatgtcac gaccggtaga cgccagcgcc gccagggtga agcgcagggc
     1501 atcggtgccg tgcggctcaa taccgttcgg gaactgcttc tcggtgcgct tacggatttt
     1561 gtccgccagc tgcggctgca tcatattgcc ggtacgtttc tccagcagtt ccggcagcga
     1621 aataccgtca accatatcca gcgggtcaat aacgttaccc ttggatttgg acatcttctg
     1681 gccttcgtcg tcacgaatca ggccggtcat gtaaacggtg tggaacggca cctgcggttt
     1741 gccattttca tctttgatga agtgcatggt catcatgatc atgcgggcaa tccagaagaa
     1801 aatgatgtcg aagccggata ccatcacgct ggttgggtgg aactggcgca gtgcgtcagt
     1861 gttttccggc cagcctaggg tagagaaggt ccacagcgca gaagagaacc aggtgtcgag
     1921 aacgtcttcg tcctgacgca gggcaacgtc ggcaccgagg ttattttcct tgcgcacttc
     1981 ttcttcgttg cggccaacat aaacgttacc tgcttcgtcg taccatgcag ggatacggtg
     2041 accccaccac agctgacgag agatacacca gtcctgaata tcgcgcatcc aggagaagta
     2101 catgttttcg tactgcttcg gtacgaactg aatgtcgccg ttctcaaccg cttcaaccgc
     2161 cggtttcgcc agtacatcgg cacgcacgta ccactggtcg gtcagcattg gttcgataac
     2221 tacgccgcca cggtcgccgt aaggaacggt caggtcgtgc ggtttaattt cttccagcag
     2281 gccgagtgcg tcaaccgcag caacgactgc tttacgtgca gcaaaacgct cgagtttctg
     2341 gaactctgcc gggatttcgc tggaataaac gtcagattcg ttacctttgg tatcgaacac
     2401 ctgggcgctt tcacggatat cgccgtcaaa ggtcaggatg ttgatcatcg gcagcgcgtg
     2461 acgtttaccc acttcatagt cgttaaagtc gtgcgccgga gtgattttca cgcagccggt
     2521 gcctttttcc atgtcggcgt gttcgtcgcc aacgatcgga atacgacggt taaccagcgg
     2581 tagaatgaca tatttgccaa tcaggtcttt gtaacgcgga tcttccgggt taacggccac
     2641 gccggtatcg cccaacaggg tttccggacg ggtagtcgca accaccagat aatctttacc
     2701 gtctgcggtt ttcgcgccgt cagccagcgg atagcggatg tgccacatcg aacctttcga
     2761 ttcgcggttt tccacttcca ggtcagagat agcggtgcgc agtttcggat cccagtttac
     2821 caggcgtttg ccacggtaaa tcaggtcttc tttatacaga cgaacgaaaa cttctttcac
     2881 cgcattggac aggccttcgt ccatcgtgaa gcgttcacgc tcccagtcga cagagttgcc
     2941 gagacggcgc atctgacggg taatggtgcc gccggactcc gctttccatt cccagatttt
     3001 gtcgatgaaa gcttcgcggc cgtagtcgtg acgggtttta ccttcttctg cggcaatctt
     3061 gcgctcaacg accatctggg tagcgatccc ggcgtggtca gtaccgacct gccatagggt
     3121 gtttttgccc tgcatgcgct gatagcggat catggtatcc atgatggttt gctggaaggc
     3181 gtgacccata tgcaaactgc cggtgacgtt cggcggcggg atcatgatgc agaaactttc
     3241 ctggctttca tcgccattag gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg
     3301 ctgttcgata tcttgtgggt tatatgtctt ttccattatt tccaggttgc cgtattcagg
     3361 ttgaaaccag ccacgcggta ggctttatag cgttcgcgcg ccagttgttt cagagaatct
     3421 tcataaggaa cgaagtctac cacttctgtg aaagcagtgg caaaatctgc aaagcttgtt
     3481 cgcagactaa tcaatatatc gcgcgggctg ctgctgcgtt tttgcggcca ggcgatctcc
     3541 accggcgcac cgccgcgcgg tccttctccc gctaaattat gcgggacaaa gctttctgcc
     3601 ggacgcgccc acagggcttc atccagccgg taagcctgct tttcatcttc acaggcgatg
     3661 agcacgcgct taccgctgcg ccaacgttct gcggcaattt cacacaccag ttgctcaacg
     3721 gcgcttaagc catcgacggt ggtgtcattg tccagaaggt agaacgttgc gtttttcata
     3781 tatggggctt cttgtggtgg atttaaatat tgctgcattc ctcaccctga aagatgagca
     3841 atgaatttct attgcgctcg atcggttccc tctcccttag ggagagggtt agggtgaggg
     3901 taaaacgcaa acttactctt cgccgttaaa cccagcgcgg tttaacagga actgtgccag
     3961 caacgctacc ggacgaccgg ttgcgccttt tgctttacca gaacgccagg cggttcctgc
     4021 aatatccagg tgcgcccagt tgtacttacg ggtaaagcgt gacaggaagc aacctgcggt
     4081 aatcgcccca ccaggacgac cgccaatgtt cgccatatcg gcaaaattgg actccagttg
     4141 ttcctgatac tcgtcaccca gcggtaagcg ccatgcgcgg tcaccggatt gttcagacgc
     4201 ggcaatcagt tcatgggcca gcggattatg gttcgccatc agaccagtaa tatgatgacc
     4261 cagcgcgatc acgcaggcac cggtcagcgt cgccacatca atcaccgctt ccggctcaaa
     4321 acgttcaacg taagttaaca cgtcgcacag taccaggcgg ccttcggcat cggtgttcag
     4381 cacttcaacg gtttgaccgg acatggtggt taacacatcg cccggacgat aggcacgccc
     4441 gccaggcatg ttttcacagc ctgccagcac gccaataacg ttaataggca gttgtagctc
     4501 tgcgaccatc cgcatcacgc cgtaaaccgc tgccgcaccg cacatatcgt acttcatctc
     4561 atccatgcct tctgaaggct tgatggagat accgccggag tcgaaggtta aacctttacc
     4621 caccagcacg attgggcgtg catcttccga cgcgttgcct ttgtactcaa tcaccgacat
     4681 cagcgattcg ttttgcgaac cctgaccgac cgccagataa gagtgcatcc ccagctcttt
     4741 catctgctgt tcgccgataa cgcgggtgat aacattcttg ctgtagctgt cagccagctg
     4801 gcgcgcttgt gaagcgaggt aagcggcgtt acagatattc ggcggcatat tgccgagatc
     4861 ttttgctgct ttaatcccgg cggcaatcgc cagaccgtgc tggatcgcgc gctcaccgct
     4921 ggtcagttca cggcgggtcg gcacgttgaa caccatttta cgcagcggac gacgcggttc
     4981 gctcttgttc gttttcagct gatcgaaact gtagagcgtc tcttttgcag tctcgacagc
     5041 ctgacgcact ttccagtagt tgttacggcc tttaacgtgc agttcagtca gaaagcagac
     5101 cgcttccatt gagccagtat cattcagcgt attaatggtt ttctgaataa cctgcttgta
     5161 ctgacgttca tccagctcac gttctttgcc gcaaccaata aggagaattc gctcggaaag
     5221 tacattcgga acatggtgca gcaacagtgt ctgccccggt tttccttcca gttcgccccg
     5281 acgtagcagg gcgctgatgt acccatcgct gattttatcg agctgttctg caatcggaga
     5341 aaggcgacgt ggttcgaaga cgcccacgac gatgcaggca ctccgctgtt tctccgggct
     5401 accgcttttt acactaaact ccatgcacta cgctcctgaa tcttaaagac aacggcggtg
     5461 gctacagata gaattgcaag ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt
     5521 aatcttaacg ttattacggc attggcacgt cagaacaaaa tctgagaggt gaatccgttg
     5581 agtataatta tcttagcgac gatttcgacg actcaagaga ataaatgacg tttaagccat
     5641 gaaacaagct aaaatcctgc aaaagacgag tttttacggg cgtatttaaa gtgataatca
     5701 taagatatct ggtgcgggag acgctcaaaa gccagctggc gatactcttc atcttgcttt
     5761 tgatcttctt ctgtcaaaag ttagtgagga tcctcggcgc agcggttgac ggcgatattc
     5821 cggcgaatct ggtgctctcc cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc
     5881 tgccattaag cctgttcctc gggctgctga tgacgctggg caaactgtat accgaaagtg
     5941 aaattacggt aatgcatgcc tgcggtctga gcaaagcggt gctggtgaaa gcggcaatga
     6001 tccttgcggt gttcacggca atcgtcgcgg cggttaacgt gatgtgggcg ggaccatggt
     6061 catcgcgtca tcaggatgaa gtgttagcag aagcgaaagc gaaccctggc atggcggcgc
     6121 tggcgcaagg gcaattccag caagcgacta atggcagctc ggtgctgttc atcgaaagcg
     6181 ttgacggcag cgatttcaaa gatgtgttcc tcgcacaaat tcgaccaaaa ggtaatgcac
     6241 gtccttctgt ggtggtggcc gattccggac atttaaccca gctgcgcgac ggctcccagg
     6301 tcgtcactct caaccaggga acgcgcttcg aaggcactgc actgttacgc gatttccgca
     6361 ttacggactt tcaggattat caggcgatca ttggtcacca ggcggtggtg ctcgacccga
     6421 acgataccga ccagatggac atgcgcacat tgtggaacac tgacaccgat cgtgcccgcg
     6481 cagaactgaa ctggcgtatc acgttggtat tcaccgtgtt tatgatggca cttatggttg
     6541 taccgttgag cgtggttaac ccgcgtcagg gacgcgtact gtcgatgctg ccagccatgc
     6601 tgctgtatct acttttcttc ctgatccaga cctccctgaa atcgaacggc ggtaaaggta
     6661 agctggaccc gacgctgtgg atgtggaccg ttaacctgat ttatctggct ttggcgattg
     6721 ttctcaacct ttgggacacc gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag
     6781 gagcggtgtg atgcaacctt ttggcgtact tgaccgctat atcggtaaaa ctattttcac
     6841 caccatcatg atgacgctgt tcatgctggt gtcgctgtcg ggcattatca agtttgtcga
     6901 tcagctgaaa aaagccgggc aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt
     6961 gctgagcgtg ccgaaagatg tgcaaatctt cttcccgatg gcggctctgc ttggggcgtt
     7021 gcttggtctt gggatgctgg cgcagcgcag cgaactggtg gtgatgcagg cttctggttt
     7081 tacccgtatg caggtggcgc tgtcggtgat gaaaaccgcc attccgctgg tcttgctgac
     7141 gatggcgatt ggtgaatggg tcgcgtcgca gggcgagcag atggcgcgta actaccgtgc
     7201 gcaggcgatg tacggcggct cgttgctctc cactcagcaa ggcttatggg cgaaagatgg
     7261 caacaacttc gtctacattg agcgggttaa aggtgacgaa gagttaggtg gcatcagtat
     7321 ttatgccttt aacgagaatc gtcgtctgca atccgtacgt tatgccgcta ccgcgaagtt
     7381 tgacccggaa cataaagtct ggcgtctgtc gcaggttgat gaatctgatc tgaccaatcc
     7441 gaaacagatc accggttcgc agacggtgag cggcacctgg aaaaccaacc tcacgccaga
     7501 caaactgggc gtggtggcgc tggacccgga tgcactctcc atcagtggtt tgcacaacta
     7561 tgtgaagtat ctgaagtcga gcggtcagga tgccggacgt tatcagctca acatgtggag
     7621 caaaatcttc cagccgctat ccgtggcggt gatgatgttg atggcgctgt cgttcatctt
     7681 tggcccactg cgtagcgtac cgatgggcgt gcgtgtggtc accggtatca gcttcggttt
     7741 tgtcttctac gtactggacc agatcttcgg cccgctgacg ttggtttatg gcatcccgcc
     7801 gatcatcggc gcactgttgc caagcgccag cttcttctta atcagcctgt ggctgttaat
     7861 gagaaaatcg taatctaaca agggagcgaa agctcccttt attattgttg acaaagtcaa
     7921 atacgagttc gaacggtccc ctcgcccctt tggggagagg gttagggtga ggggcacaga
     7981 tcacgtctga cttacctttt tctccccccc agcaaactcc ccaacatccc gcgtacaatc
     8041 tgattcgtca cctggcgagc ggcgcttttc gccattgttt gcaccacacc atctttcttc
     8101 ccgccgcgtg gtccggtagt gccaaacaaa atatccttca atccaccaag aataccgtca
     8161 tccaccgcca cctctttccc tttcacgggg ggattatttt gctgctcggt actggcctga
     8221 aagccttttt gcagcatctc ataggcggac tcgcggtcca cctcatcttc atatttgcca
     8281 tacaccgggg agtgattaat caggccatta cgctcatctt ccgtcaccgg ccccattcgc
     8341 gaacaaggtg cgatcaccat cgcccgttcc accactgaag gacttccttt cgcatcgaga
     8401 aacgagatta acgcctcgcc ggtccccagt tcctggattg ccttttcggt atcaaacgcc
     8461 ggattggccc gcatggtttg cgccgctgcc ttcactgctt tctgatcttt aggcgtaaaa
     8521 gcacgcaaag cgtgttgaac gcgattaccg agctgcccta gcacgttatc cgggatatct
     8581 gacgggtttt gcgaaacgaa ccagacgccc acgccttttg agcgaataag ccttatcacc
     8641 tgctcaatct tatccagcag tacctgtggc gcatcgttaa acagcagatg tgcttcgtcg
     8701 aagaaaaaca ccagttttgg tttctccaga tcgcccgctt caggcaattg ttcatataac
     8761 tctgaaagca tccacagcag gctggcggcg tacagtttcg gcatctgata aagtttctcg
     8821 gcgctgagga tattgataac gcctttaccg ttggcatcgg tgcgcatcca gtctttgata
     8881 tccagcatcg gctcaccaaa gaagtgttct gcgccttgct gttccagcga cagtaatccg
     8941 cgctggatgg caccaaccga tgcgctactg atatttccgt actgattctg gaaggatttg
     9001 gcgttatcgc cgatgtactg ggtaatcgcc cgcaaatctt taaagtcgag cagcaacaat
     9061 ccctgatcgt ccgcaatgcg gaagataata ttcagcacgc cagattgcac atcgttgaga
     9121 ttcaacagtc gtgccagcaa cagcggcccc agatccgaaa ccgtcgcccg caccggatgg
     9181 cctttctcgc caaagatatc ccacaccacc accggattgg catgcggttg ccagtcgttg
     9241 acgccgatat ttttcagtct tgcgagcagt ttttccgacg ccgttcctgc ctgcgcgatg
     9301 ccggtcagat cgcctttcac atcggccata aacaccggaa caccgatttc cgacaatgac
     9361 tctgccagtt tttgcaacgt gacagtttta cccgtccccg ttgcgccagt aatcagcccg
     9421 tgacggttag ccattcccgg cagtaaaaac agttctgtgt ccggcgtgcg ggcaattaac
     9481 aggggttcgc tcatgagatt tcctccagtt tcctgcctgg agtataggca acgtccggca
     9541 aagagaatat gtgtaatgaa gagaaaataa cctaaagcgt gttaccgtga taaatctgat
     9601 acccgaggtc aacagtgttg tggttgtgat cgttattctt aatcttgctc agcaacattt
     9661 gggcggccat ccgaccaata tcaaaacggg gagtaattac gctggcgagg cttgggatca
     9721 tttgcctgcc aatctccagc ccatgaaaac ccgcaatgga aatttgttct ggcacggcga
     9781 ggtttcgctc gcggcacagc agaagcgcac ccatcgcaat atcgtcattg gtgcaaaata
     9841 caccgtctaa atccgggttt gcgctaaggg catcacgcat taactgcatt cctaaatgaa
     9901 tggatgagat ggcgcgtgga ttcatgcgta atggggaaag attatgcagc atcatcgcat
     9961 cgcaataccc ctgataacgc tgttcatcgc gggtgtcatc tttggaaccg agatacagaa
    10021 ttttgcgcct gactcgctta tccagcatgg tacacaccat gtcaaaagca gcctgccgat
    10081 tatcaaaacc gacctccata tccagccgtt ctccctgtac atccatcaat tccacgaccg
    10141 gaatggtggc tgaacgcaga aatttcaccg tcctgatggt gtgatatttt tccgaaagaa
    10201 taatcccgtc aatgttataa gagagcaggt taatcaccga ctcttcttcg gaatcgcgat
    10261 cgtaattgta attagcaata agggtctgat aattatgcac agaggtgacg gattctattc
    10321 ccgcgagaat gtcagcgaag agttgatttt gaaatgacgg aatcaagata ccgagggtat
    10381 aactttgtgc attcaacaac atgccgggag cgcgattagg gatataattg atctcttcca
    10441 taattttggc gatgcgctcg cctgtttcct ttgccacctt tttcggcgag cggatataac
    10501 gactcacggt catttttgtt acgccagcca gcgtagcgat atcctgtaaa gaaattctgt
    10561 gattgcgcat aacttctccc aacgtcgggg gaatcttcaa tcttcccccg aaactacatc
    10621 acaatatttt attctttcag tgcaatacgg cgttaatggc taacacgccc agcaaaccca
    10681 taatagaaat aagggtttcc atcactgtcc aggtacgcaa cgtttcacca accgtcagat
    10741 taaaataccc tttaaatagc cagaagccag ggtcgtttac gtgtgacgcg atcacgctgc
    10801 ccgcaccagt tgccagtacc attaaagcgg gatcggcatg ggtaacgttg ataatcggca
    10861 acaccacacc cgcggtggta atggccgcga cggtggcaga gcccagagcg atacgcaaca
    10921 gcgccgcgac agtccagcac atcaataacg gcgaaagcgt agttccggtc attaagtgtg
    10981 aaatatactg cccgacacca ctatctacta atacctgctt aaacgcgccg ccgccagcga
    11041 taataaacac aatcatcgcg atagcgccta tagaatcccc aatgatatcc atgatttgct
    11101 cgatggtgcg tccattacgt cggcccagtg tgaaaatcgc aataacaatg gcaataaaca
    11161 gcgcaacggc agggttaccg acaaattcaa agaagaggcg cacggtgtta gttttcggta
    11221 acgtaatttc acaaacggcg gcgatagcca tcaggatgac cgggatcact gcagcaaaaa
    11281 tactgttcca gaaggagggc atctcctctt cgctaaacag atgaggatta aataagcctt
    11341 ccggtggtgc tttctcaaag cgagttagca gtttagaaaa cagcggtcct gcgacaataa
    11401 ctgtcggaat ggtaatgata aatccataca gtaaagtcgt tccgagatta gcctcaaaga
    11461 tagtcgcgat ggcagtaggc cctggatgtg gcggcagaaa acagtgggtt acagagagcg
    11521 ctgctaccat cggtacgcca acatacaaca ggggtaatcc agatgatgcc acgatggtaa
    11581 ataccaacgg caacagcagg acaaaaccca cttcaaaaaa catggcgagg cccacaacca
    11641 gaccggtgat cactagcgcc cattgcacgc gttttttacc aaaagtagca atcagcgtag
    11701 tggcgatacg ttgtgccgcg cccgtatcag aaatcagctt gcctaacatg gccccgaaac
    11761 cgaggatcat tgccagcccg ccgagcgtgc tgccgatacc attttgtata gagtgcagga
    11821 cggcctgtgc atccatccct tcggcaaatc cgacgacggc agctaccaga acgagggcaa
    11881 taaagccgtt aactttaaag ccgatcatca ggataagaag cagcgcgacg cctgccgcaa
    11941 taatgattaa tggcatagtc tttatctctt attagtgcca gaaaagcagc gctttcgcgc
    12001 tgcacgtagg gtggtgggta gggtggtggc tgttaaacag ccactaacat gccgccatca
    12061 acaaacaaca ggtggccgtt tacgaaatca gaggcttttg aagaaaggaa caccgcagca
    12121 ccaatcagtt cctgcggatc tccccagcgt gcggcggggg tccgtttgca caaccaggcg
    12181 gtgaaggctt cgtcctcaac cagtgcttta gtcatttctg ttttgaaata gcccggcgca
    12241 ataccgttga cctgaatatt gtggcgcgcc agctcgacgc acatgccgcg ggtgagcatt
    12301 tttaccgccc ctttcgatgc ggcataaggg gtgatggtgt cacgtcccag ttcgctttgc
    12361 atcgagcaaa tattaataac tttacctgcc ttgcgttcaa ccatgtgacg agttaccgct
    12421 tgcgatacca ggaacacggc ggtctggttt actgcgatca catcattcca ctcttgttca
    12481 gggaactcag taaaaggatg acggcgctgg ataccggcgt tattcaccag cacatcaatg
    12541 gggccgatgt ccttttcgat atgttcaacg gcggcatcaa tttcatgttt atgagtaaca
    12601 ttaaaaggtg cggcaacggc ctgaataccc tcctggtgga gtttttctac agcaagttct
    12661 gcgcgttcgg cagtaatatc attaataatt atttgtgcgc catatttacc caggccggtt
    12721 gccagtaaat agccaatgcc ctgtgctgaa ccggtaatca agatattttt tcctgccagt
    12781 gaaaatagat cgttcattgt attattcctt tatttatctc ttagaaaaca agctggactt
    12841 ttgctgcctg ggttttatca ccggcgaaac gtagcgcctc ttccaggtca gtgaagagat
    12901 attcagcact cagtaaaggc agtggattga taacgccatt cgccagccat gacactgcgg
    12961 tattaaattc gctggtaaaa cggaaagagc ctttgagtga aatctcctta ccaatcaacg
    13021 tcatcattgg gaattctgcc atcgcgcctc ccatccctac ctgcaccatt acgccgcgtg
    13081 cacgagtgac ctccagacag gtattcactg atgaaggatg accggacact tcaaagctga
    13141 catcgaaatg gcctttttcc gctttccagt gatccatgtc gtcgttttgt gggtttacga
    13201 gcacatccgc acccatctct ttggccagcg aaagggaacg gggactcaca tcggcacaga
    13261 caatttccgc ggaccccagt gttttcacag cactgacaat caggcagcca atgggcccaa
    13321 caccggaaac aaatactcgc ttgccctgta actcgtcggc ctgatgtgcg gcatgaatcg
    13381 cgacggctaa aggttcggca aaagccataa ccttttcgtc agctttggcc ggataaggga
    13441 cacattgcga cgtttcgacc attttataac gggtaaaacc accatcaaca tgagggaaat
    13501 acatggcact gccaaaaaaa cgcatatctg tacactgatt ctcgttatgt tcaatgcagt
    13561 atttgcagtg accgcacggt ttagacggat taatggctac cgtttgccct tcatgtaatt
    13621 ctgatgagtc gctatgaata actttaccga taacttcatg acctaacacc atcggtgcct
    13681 ttatcatgaa attacctact tttccttcct gataataatg taaatcggaa ccgcaaattc
    13741 cacctcgggt tatttgtact aatgttccat tattattcca atctatcgtc tgctcggtaa
    13801 cggcaacagt tttcttgtcc gcaacaacgc aggactgtgt tttcacttgc ataaattcct
    13861 cactggtcag gtagacacct cggaagcatt taagcggttt taattgtcat tatttgtgat
    13921 gaagatcacg tcagaaaatt gttacattac tatgttacgc ataacgtgat gtgccttgta
    13981 attcttatca gcagaaataa aaaaacgtga aattattatg ccaccaggcg tagtatcgca
    14041 gcaggtaaga tgattcagga gattttaaat ggcgggtgaa agctttattt tgatgggcgt
    14101 ttcagggagt ggtaaaacat taattggtag caaggttgcc gcgttattat ctgctaaatt
    14161 tattgatggt gacgatcttc atccagccaa aaatatagat aaaatgtcgc agggtattcc
    14221 attatctgat gaagatcgac ttccctggct ggaacgctta aatgatgctt catacagtct
    14281 ttataaaaag aatgaaacag gatttattgt ctgttcatca ttaaaaaaac agtatcgtga
    14341 tattttacgc aagggtagcc cccatgttca tttcctctgg ttagatggcg actatgaaac
    14401 tattctcgcg cgaatgcagc ggcgggctgg gcattttatg ccggtagcgt tactaaaaag
    14461 tcagtttgag gcgctggagc gtccacaagc agatgaacag gatattgtac gcattgatat
    14521 caaccatgat attgccaatg tcaccgaaca atgtcggcag gccgtgctgg cgatacgaca
    14581 agacagaata tgtgcgaaag ggggtagcgc ctcagatcag cgctgcgaat gattttcaaa
    14641 aatcggcttt cagaaccacg cggtaacgcg ccttaccgtt gcgcacatgc tggatggcgt
    14701 cgtttatttt cgacatcggg aacagttcgg tggtcggcgc aaccttgctg cgggccgcaa
    14761 agcgcatcag cttacgaagc tcataaggtg tgccggttgc tgaaccagag acgctgcggt
    14821 cgcccgcaat taacgtaaag gccggaacag acagcggcgt gaggaccgca ccgacggtgt
    14881 ggaaattacc gccatacgtc agcgcctcaa aataaggctg ccagtcgagg ctgacgttga
    14941 cggtgttgat aatgagatcg aactgccccg caagtgtttt cagtgcctgc ggatcgcggc
    15001 tattcaccac tttatcggca cccatcgcca gtacttcctg ctctttcgca ggattcgaac
    15061 taaaggctgt cacctcgcat cccattgcgt gcagaagttt tatagcgata tgccccagcc
    15121 cgccaatacc aattacccca acgcggctgg tagcagtgat atggtgcatc aacagtggtt
    15181 taaagaccgt gataccacca cacagcagcg gcccggcgga ctcgatatcg atattttccg
    15241 gcagtgggat cacccattgc cagtctgcac gcaacttctc ggcaaaaccg ccgcgattca
    15301 taattgttgg caccgcacct tgctcgcagt tgatctgatt accgctaata caggcgtcgc
    15361 aatgcccgca gctacgcgcc gtccagccaa tcccgacacg ctgaccgacc tgcaaacctt
    15421 tatcctgcgc ggcgctcccg agcgccacca cgcgcccaat cacctcatgc ccggcaacca
    15481 gcggatactg tgaaaatccc cattcgttgt cgatcatcga cagatcggaa tggcagatcc
    15541 cgcagtaatc cacctgcact tcaacatctt gtggcttcaa ttcaccgggc tcgtactcat
    15601 aaacttccag ttcgccgccc gcttcttttg cggcatagct ttttatcatc gacatttttt
    15661 ggtccttctc tggtgttgtc tgggaagtgt agagcatggc agggcggggt gcctggagtg
    15721 tgacaaaggt tacacatcgc tgtatgcaat gctgaaaatt tcagcactta gcgaggtgcg
    15781 agcaagctgg cgcttgcatg gtggcgtgcg acaggtataa tccacaacgt tttccgcata
    15841 cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt gattcaaaat caaccgtaga
    15901 aatacgtgcc ggttcgagtc cggccttcgg caccaaaagt atgtcaatag acctcaactg
    15961 aggtcttttt tatgcctgaa attcagtatt tatctacctt tcccgctata ttaactctct
    16021 caaggtcaac cgatatcaac gtacttctac caacatatgt tggtacagat gatggtattt
    16081 ccggttcgat aatgcttgta ccaacaggga gggaatacgc atggcattaa cagacatcaa
    16141 agtcagaaca gctaagccaa cagataagca atataagttg actgatggtg gcggtatgca
    16201 tctgcttgtc catccaaatg gttctaagta ctggcgtttg cagtaccgtt atgagggaaa
    16261 gcaaaagatg ctggcacttg gggtttatcc tgaaatcaca ctcgcggatg ccagaatacg
    16321 tcgtgacgag ccacgtaagc tgcttgcgaa tggcgtcgat ccgggagaca aaaagaaaag
    16381 tgataaggtt gaacagagta aagcacgaac ctttaaagaa gtcgcgattg agtggcatgg
    16441 caccaataaa aagtggtctg aagatcatgc ccatcgtgtg ctaaaaagtc tggaagataa
    16501 tctttttgca gcgcttggtg aacgtaatat cgctgagtta aaaactcgag atttattagc
    16561 acctattaag gccgtagaaa tgtctggacg tcttgaagtg gctgctcgtc ttcaacagcg
    16621 c
//
LOCUS       DAZOYS010000083        16400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOYS010000083 DAZOYS010000000
VERSION     DAZOYS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.083"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHUN9_002345"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHUN9_002345"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0695723.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHUN9_002346"
     CDS             complement(2380..2820)
                     /locus_tag="ACHUN9_002346"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0695724.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUN9_002347"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUN9_002347"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0695725.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHUN9_002348"
     CDS             3538..4236
                     /locus_tag="ACHUN9_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0695726.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHUN9_002349"
     CDS             4465..5673
                     /locus_tag="ACHUN9_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0695727.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUN9_002350"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUN9_002350"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0695728.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHUN9_002351"
     tRNA            7780..7856
                     /locus_tag="ACHUN9_002351"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHUN9_002352"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHUN9_002352"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUN9_002353"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUN9_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0695729.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUN9_002354"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUN9_002354"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0695730.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUN9_002355"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUN9_002355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0695731.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUN9_002356"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUN9_002356"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0695732.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUN9_002357"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUN9_002357"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0695733.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHUN9_002358"
     CDS             complement(14640..15911)
                     /locus_tag="ACHUN9_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0695734.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4093 c         4409 g         3998 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta atagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAZOYS010000084        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOYS010000084 DAZOYS010000000
VERSION     DAZOYS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.084"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUN9_002359"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUN9_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0695735.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUN9_002360"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUN9_002360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0695736.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUN9_002361"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUN9_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0695737.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUN9_002362"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUN9_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0695738.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHUN9_002363"
     CDS             6397..6507
                     /locus_tag="ACHUN9_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0695739.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUN9_002364"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUN9_002364"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0695740.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUN9_002365"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUN9_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0695741.1"
                     /translation="MKPSSSNSRSKGHAKVRRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHUN9_002366"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHUN9_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUN9_002367"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUN9_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0695742.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUN9_002368"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUN9_002368"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0695743.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAGELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHUN9_002369"
     CDS             13514..14446
                     /locus_tag="ACHUN9_002369"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0695744.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHUN9_002370"
     CDS             complement(14487..15917)
                     /locus_tag="ACHUN9_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0695745.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3804 a         4098 c         3941 g         4205 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cacttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact ctcccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZOYS010000085        15995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOYS010000085 DAZOYS010000000
VERSION     DAZOYS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.085"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(78..230)
                     /locus_tag="ACHUN9_002371"
     CDS             complement(78..230)
                     /locus_tag="ACHUN9_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0695746.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(495..1229)
                     /gene="cysH"
                     /locus_tag="ACHUN9_002372"
     CDS             complement(495..1229)
                     /gene="cysH"
                     /locus_tag="ACHUN9_002372"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0695747.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHUN9_002373"
     CDS             complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHUN9_002373"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0695748.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHUN9_002374"
     CDS             complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHUN9_002374"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0695749.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5134..5496
                     /gene="queD"
                     /locus_tag="ACHUN9_002375"
     CDS             5134..5496
                     /gene="queD"
                     /locus_tag="ACHUN9_002375"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0695750.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5574..6845
                     /locus_tag="ACHUN9_002376"
     CDS             5574..6845
                     /locus_tag="ACHUN9_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0695751.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6836..7096
                     /locus_tag="ACHUN9_002377"
     CDS             6836..7096
                     /locus_tag="ACHUN9_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0695752.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7113..7688
                     /locus_tag="ACHUN9_002378"
     CDS             7113..7688
                     /locus_tag="ACHUN9_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0695753.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7836..8696)
                     /locus_tag="ACHUN9_002379"
     CDS             complement(7836..8696)
                     /locus_tag="ACHUN9_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0695754.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8693..9472)
                     /locus_tag="ACHUN9_002380"
     CDS             complement(8693..9472)
                     /locus_tag="ACHUN9_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0695755.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9450..10400)
                     /locus_tag="ACHUN9_002381"
                     /pseudo
     CDS             complement(9450..10400)
                     /locus_tag="ACHUN9_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10387..11820)
                     /locus_tag="ACHUN9_002382"
     CDS             complement(10387..11820)
                     /locus_tag="ACHUN9_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0695756.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11890..12706)
                     /locus_tag="ACHUN9_002383"
                     /pseudo
     CDS             complement(11890..12706)
                     /locus_tag="ACHUN9_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13024..14301
                     /locus_tag="ACHUN9_002384"
     CDS             13024..14301
                     /locus_tag="ACHUN9_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0695757.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVFYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14328..15814
                     /locus_tag="ACHUN9_002385"
                     /pseudo
     CDS             14328..15814
                     /locus_tag="ACHUN9_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3761 a         4220 c         4034 g         3980 t
ORIGIN      
        1 ataatataaa ttaaaaaagg gtgcaggaga ccaaaccatc acaattcagt acgcggggtt
       61 ttacccccgc gtggtggcta cttcttcgat tcgtaagcga gaactgcctt aaactccata
      121 ccacgttctc tgatactcaa ctcacacaac gagtcgccta ccatcagcgt gaatcccagt
      181 actgtaaaac acagcactat gattgccaca agggcatatt ttgtcagcat gtcttgcctc
      241 cctgcaaaga agagactaac attcgaattg tttgggttcg aacgctggcc tcaggttgat
      301 agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa cgccagacag
      361 cctcaagcac ccgacgccat tctatacctg ataattcttc cagcggcgtt cacgtttaaa
      421 atcaggaata ctgctcgcaa aataaaaacg cccccatcat ttctgacaga ggcgtttaat
      481 ttatccggca atatttaccc ttcgtgcaat ccacattccc tttttaagcc aaagaaacgt
      541 gtttcttctt ccgacatgcc aggttcccat ttacgggttg tatgggtatc accgaccgat
      601 aaatatcctt catcccataa tgggtgatat ttcaggccat gtttttgcag gtactgataa
      661 atagttcggt tatcccagtc gataatcggc agcactttaa atacgccacg ctgaattgcc
      721 agcaccggta aattggcgcg actgccggat tgttcacggc gcaggccagc aaaccaggtt
      781 tgcgcgttaa gttctttcag agcccggttc atcggttcga ctttgttgat gtcattgtac
      841 ttttcaatgc cttcaacgcc ctgctcccac agttttccgt agcgtgcttc ctgccaggct
      901 gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt taactcgtca
      961 ataaagcggt aggtttccgg gaacaagtaa cccgtatcgg tgaggatcac cggaatatcc
     1021 gggcgaattt gattcaccag atgcaggctc accgccgcct gaatgccaaa gctggaagaa
     1081 agtacatatt caccgggcag attatccagc gcccaggcta cgcggccttc agcgtccagt
     1141 ttttccagtt cggcgttagt ttccgccagc gccagaatac gatctacctt cggcagttcg
     1201 ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt gcctgatgcg
     1261 acgctcacgc gttcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1321 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1381 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1441 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1501 caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc gccagcatcg
     1561 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1621 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctctgctt
     1681 ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt tcacgctgcg
     1741 gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1801 tttccggtac accggtgatg atcagattct ggttcgccgt aatgcggaaa tcgcctttgt
     1861 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1921 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1981 tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg atccccgcgc
     2041 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2101 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttcgg
     2161 ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg cgggcgtagg
     2221 ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg aagcctacca
     2281 gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga tcgatatcgt
     2341 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2401 tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc gcatacgcgc
     2461 gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct tccgcgtgca
     2521 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2581 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2641 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2701 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2761 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcac
     2821 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc
     2881 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2941 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     3001 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3061 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3121 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3181 gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt gtacataaac
     3241 tttttctttt tggtcgcgcg accaggcaag atcgatacgc gtcagcacgc cctctttgac
     3301 gtagcgctgc cactccacct ggtagaggaa atcttccgta aagtgcgggt tgccaaagaa
     3361 cagccagttt ttacccggcg cttcgtcggc ggcgcgttgc tgcataaagg cacggaacgg
     3421 cgcgatgccg gtaccggggc caatcataat cactggcgtt tgcgggttag cgggcaggcg
     3481 gaaattgtcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcgatccgc
     3541 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc
     3601 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg
     3661 cggggtcagc gggcgcagca ggttaatcag cgcttcggca tccagttgcg ccggagagaa
     3721 acgcaccatg tcgacaatcg gcgtggtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3781 caccagcggc agcagcgttt cgctgcgggt aagcgtggca tagttctcaa caatgttggc
     3841 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3901 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3961 tgccgggtcg ttctgatacc agacacccag cgcgtcaccc ggctggtaac gcaggcctga
     4021 atcgcctaag tcaatttcga tatgacgaac gtctttttca gagttacgcc cggtaatttt
     4081 ctggttaacc gaaaggctcg ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat
     4141 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4201 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4261 atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt cgaaatcttt
     4321 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt
     4381 gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4441 cggcggttcc ccttcccctt gcgttgacgt cactacgatg agcagttttt cgctggcgat
     4501 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ctgctaataa
     4561 atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg cggagataat
     4621 agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag caggctgctg
     4681 attgagtacg ccccagaaat agccagaaac ccaggcaagc tgggtaggcg ttaaatcggt
     4741 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4801 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4861 gcgcccataa aacagaagag aagataaggt taacggggtg aacggtgtgg attaaagacg
     4921 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4981 aaatagatta accaacccaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
     5041 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5101 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5161 accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg tggtcgcctg
     5221 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5281 tggattatcg atttcgctga actgaaagcg gcgtttaagc caacttatga gcgcctcgat
     5341 caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt tttagcaaaa
     5401 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5461 acctgcaccg cagggtgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5521 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5581 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
     5641 gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa
     5701 atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg caatttcatc
     5761 tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta acgcccgatg
     5821 gcgtaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5881 ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc atccccggag
     5941 cgacggtgga tgcactgtat gaagaaaacg gcagagtctg tggcgttatt tgtggtgacg
     6001 atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac
     6061 gtcacgggtt agtgactcgt cctgctggcg aagcgatggc gttggggatc aaagaagtgc
     6121 tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6181 cgttgctgtt cagcggcgga atctgtgatg acttacccgg cggcgcattt ctttatacta
     6241 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagtcgtg
     6301 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca
     6361 aaaacacgga atcactggag tatggtgcgc atctggagcc agaaggtggc ttgcacagta
     6421 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6481 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgca caggcggcgg
     6541 cacaaacgct aataagcgcc tgccagcacc gcgagccgca aaatctgttt gcgctttatc
     6601 atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag catgttccgg
     6661 cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag gatatttccc
     6721 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcgtc
     6781 atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
     6841 tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
     6901 tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt tttcgctcac
     6961 accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca cctgccgttt
     7021 gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7081 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacaccta ctccgccaga
     7141 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggctatc gattctgaat
     7201 gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc gttaaaaaga
     7261 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7321 aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc ggacggcatt atcagtacca
     7381 aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat cgcctgttta
     7441 ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7501 gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca gagaaaatcc
     7561 gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7621 acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7681 tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc cgcacgccac
     7741 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7801 ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc gtgaatgttt
     7861 gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa
     7921 aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7981 gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg tgccagcaga
     8041 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
     8101 ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc
     8161 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc gcgagtaaca
     8221 tttttcagat cctctgtgct aaccagccag tccggaagcg cacccgggac aatgttcagt
     8281 tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg cgccagggaa
     8341 aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat
     8401 ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt
     8461 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
     8521 acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg ttccgccact
     8581 acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc ctgcgccgcc
     8641 agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
     8701 gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct accgtttgcc
     8761 catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga cactgcatcg
     8821 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
     8881 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
     8941 gcaagcgaac ccggcaacaa cgcaacccat gttcagtacg ctgttcgagg gtgataaaca
     9001 gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc atttccgcca
     9061 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
     9121 ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9181 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
     9241 gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9301 cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
     9361 ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc cactcttttt
     9421 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9481 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
     9541 acaacaaaat caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
     9601 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
     9661 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9721 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9781 aatgaactgc cggaaggcaa acaggccatc actaccagcg ttgccgccag cagcaaaaaa
     9841 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg
     9901 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9961 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
    10021 aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    10081 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    10141 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggtgattct
    10201 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagtagc
    10261 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10321 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10381 gaaaattcag ccagcaaggt taatgccatt aggatcgaac tgctttttca gcccttccag
    10441 caacgcccac gcgctgccgt gttccagttt gctccagtga acgcgatgtt taccgatacc
    10501 gtggtggtgc accatcgaac cgccgaggcg aatggtttct tcacagatga tcttgttgag
    10561 cggattgtgg tacttgtcga tttcctcttc cggcttacag tcaacgacgt tgtaatcgta
    10621 gacgaagtac atgttggtgc cgttctgata gctatgagag gaatgaccgc ccagcatggt
    10681 gatgtcgtcg gcgtgcggga actcagtacg aatacggtta ataacgcttt cgtagatttc
    10741 gtggatgcag ctccagcagc cggacacttc agtggtaaag cccatgttgc cggttttgag
    10801 gatctgcaca cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt
    10861 ttcgatcagc ttgctgtcca cgcgctggca ttgcgggtag cgggcaacga tttccgcaat
    10921 cccttcgccc gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacgca
    10981 ttttccgtct gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc
    11041 gatcgacgga cgataccctt ccaccatgac ttcacgcagg atgttgaagt cggttttcat
    11101 gtcttccagg atatagccgt agaagaggtt gttttccggg gtgaatttaa agattttcac
    11161 tgttacttca gtgatatagc aaagtgcacc ttcgttaccg atgatgatgt ggcgaatgtc
    11221 cgggccagcc gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat cagccaatac
    11281 tgcttccaga ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat
    11341 actgcgggtt gctaccaagc cgcccatctg cgccagcggc tttgactgcg gagaatgccc
    11401 cgtggtgtaa cctttttcac gcaacgcatt ttccagcacc tccagcggaa cgccacattg
    11461 cgccgtcgcc tgcatattct caatatcaat attaatgatt tgattcatgg cggagccgtc
    11521 aagcaccacc gagttttcca caacggtttc cagcccacct tcagtggcgg aagcgccggt
    11581 acgcggcaca ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttgctc
    11641 tgtggaaccg agttttacga ccgctgccgg aatcggcaaa gtataaatgc catgaatatc
    11701 cggaaattta cgaaaacggt caatactgtt cttctttaat actgtttcat cggtaattac
    11761 gcgatctgca ccaacaattt cctttagttg gtcgacaatc gctgcgcgag ataaagacat
    11821 agtaattcct tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac
    11881 cgttatggat tagcgcacta aataaccacc attgaccact aataaatgcc cattgacata
    11941 atttgaagcc ggacttgcga ggaatacagc tgcgcccatt aaatcctgag tatcgcccca
    12001 acggtttgcc ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc
    12061 cagcgtaata tctgttgcat aatagccagg agcaatacca tttacctgaa tattatattg
    12121 acctagttca tcacaataag ctttggtgaa tccggcaaga gcatgtttag tggcagaata
    12181 tgcaggtgac cattgtccac ctaagtaaga gaacaatgaa cagatattaa taattttgcc
    12241 gcttttctgc gggatcataa tttttgcagc ttcatagctt aactcgaatg cggcggtcag
    12301 gttcacatca atcatcggat cccagtcggc acgaccgaag tccagcacct tattcagctt
    12361 acaaataccg gcattgttta ccagaatatc aactgtaccg aaacgctcac agcaagcagc
    12421 gataatcttc tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt ccacctcaac
    12481 accctgtttt tcaaacattt cctttgtttc gccgttatct ttcaatcatt tcctttgttt
    12541 cgccgttatc tttgacgaaa ctaggaataa agatatttgc gccagctttg gccaacgcca
    12601 tggcaaatgc ctggcctaaa ccgctattcc caccggtaac aattgcggtt ttacctttca
    12661 gtgagaaaaa atccattgag aacgcattga gagattcgat tgacatagtt agctccaatt
    12721 tccgtaaggc aaaaaaaaga aaggtaactt cccccgcgag agggaagtta cctttctcat
    12781 tatctctggt aactggagat aatttttagc ataacggcat aactgctaat gtgatcgtaa
    12841 tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag cagaagcgtg attactctca
    12901 cacaacacct ctgctttcca tgttatttct tattgcaatt actagagacc tgagaaaagt
    12961 aatccacctg aaagggcggc ttacttttct cttttttttg tctattacaa atgggaatac
    13021 gatatgcaac acaactcata tcgccgttgg ataaccctcg cgataattag ttttagcggc
    13081 ggcgttagtt ttgacctggc ttatttacgt tatatttatc aaattcccat ggcgaaattt
    13141 atgggattca gcaataccga gataggttta ataatgagta cctttggtat tgcggctatt
    13201 attctttatg cccccagcgg cgttattgcc gataaatttt cacaccgcaa aatgattact
    13261 tccgcgatga tcattaccgg attactgggt ctgttaatgg caacgtatcc accgctgtgg
    13321 gtaatgctct gtattcaggt cgcctttgcg ataacgacga ttttaatgct gtggtcggtg
    13381 tcgattaaag ccgcctcgtt gctgggcgat catagtgagc aagggaaaat tatgggctgg
    13441 atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg tgtttaccat gtgggtcttt
    13501 tctcgctttg caccggatga cagcgccagc ctgaaaacag tcattatcat ctacagtgtg
    13561 ttttacatct tgttggggat tctgtgctgg ttttttgtta gcgataacaa caacctgcgc
    13621 agtgccaata acgaagaaaa acagtcattc cagcttagcg acatccttgc cgttttgcgt
    13681 atcagcacca cctggtattg cagcatggtg atttttggtg tcttcacaat ctacgccatt
    13741 ctgagttact ccaccaacta tctgaccgaa atgtacggca tgtcgctggt ggcagcgagc
    13801 tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt gcggcccact tggaggcatt
    13861 atcaccacct acagtaaagt gaaatcccct acccgcgtga ttcaaatcct ttccatcatc
    13921 ggtttgctgg cgttgacagc cctgctcgtc acgaactcta acccccaatc ggtcgcgatg
    13981 gggattggcc tgattttact gctgggattc acctgttacg cctcacgcgg gctgtactgg
    14041 gcctgccccg gcgaagcgag aacaccgtct tacattatgg gcaccacggt aggtatttgt
    14101 tcggtgattg gattcctgcc ggatgtcttc gtttacccga ttatcggcca ctggcaagac
    14161 accctgcccg ctgcagaagc ctaccgcaat atgtggctga tgggcatggc ggcgctcggc
    14221 atggtgattg tctttacctt tttgctgttc caaaaaattc gtactgctga tagcgccccc
    14281 gcaatggcta gcagcaagta agccaggaaa attacaggag aaaaatcatg tcgaaaaaat
    14341 acatcatagg gattgatggc ggaagtcaga gcacaaaagt ggtgatgtac gatctggaag
    14401 gtaatgtggt ttgcgaaggt aaaggcttat tacagccgat gcacacgcct gatgccgata
    14461 ctgcagaaca tcctgacgac gatttatggg catcattatg ttttgcaggt cacgatttga
    14521 tgagccagtt tgccgggaat aaagaagata ttgttggcat cggcctgggc tccatccgtt
    14581 gctgccgtgc gttattgaaa gccgacggca cgccagctgc gccgttgatt agctggcagg
    14641 atgcacgcgt tacacgccct tacgaacata cgaatcctga cgtggcgtat gtaacctctt
    14701 tttcgggtta tctgacgcat cgcttaaccg gcgaatttaa agataatatc gccaactatt
    14761 ttggtcagtg gccggtggat tataagacct gggcatggag cgaagatgcc gcagtcatgg
    14821 agaagtttaa tatcccccgt catatgctgt ttgatgtgca aatgccaggc actgttcttg
    14881 ggcatatcac acctcaagcc gcgctggcga cgcatttccc ggcaggactg ccggttgttt
    14941 gtaccaccag tgataaaccg gtagaagctc tgggagccgg attactggat gatgaaactg
    15001 cggtaatttc tcttggcact tacatcgcat tgatgatgaa cggcaaagcc ttgccgaaag
    15061 atccggtggc gtactggccg attatgtctt ctattccgca aacattgctg tatgaaggtt
    15121 acggtattcg caaaggcatg tggacggtga gctggctgcg cgacatgtta ggcgagtcgt
    15181 taattcagga tgccagggcg caggatcttt caccggaaga tttactcaac aagaaagcct
    15241 ctagtgtgcc acctggctgt aatgggctga tgacggtgct ggactggctg accaatccgt
    15301 gggaaccgta caaacgcggg attatgatcg gctttgattc cagcatggat tacgcatgga
    15361 tatatcgttc gatattggaa agcgtggcgc tgacgctgaa gaacaattac gacaatatgt
    15421 gtaatgaaat gaatcacttt gcgaaacatg taatcattac tggcggcggt tcgaacagcg
    15481 atctgtttat gcagattttt gccgacgtgt tcaaccttcc ggcacgccgt aacgccatta
    15541 acggttgtgc aagcctgggg gcagcgatta atacaggggt aggtctgggg ctatacccgg
    15601 attacgcaac ggctgtcgat aaaatggttc gcgtgaaaga tatctttata ccgattgaga
    15661 gcaatgccaa acgctacgac gcgatgaata aaggcatttt caaagaccta accaaacata
    15721 ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg aattggggaa
    15781 tgcggattcg atccagagct ggtcgaatgc gtgaataagg taggccgggc ggaaaactgc
    15841 ccggcctgaa catacctgaa tggttatccc cgctggcgcg gggaacataa gttcgacaac
    15901 ttaaaaaatg ataacaagtc cggtttatcc ccgctgatgc agggaacata ataaaaactt
    15961 taactagttc ggtttatccc tgtttttaca aggaa
//
LOCUS       DAZOYS010000086        15964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOYS010000086 DAZOYS010000000
VERSION     DAZOYS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.086"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..456)
                     /gene="spy"
                     /locus_tag="ACHUN9_002386"
     CDS             complement(<1..456)
                     /gene="spy"
                     /locus_tag="ACHUN9_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0695758.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAA"
     gene            complement(786..1754)
                     /gene="astE"
                     /locus_tag="ACHUN9_002387"
     CDS             complement(786..1754)
                     /gene="astE"
                     /locus_tag="ACHUN9_002387"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0695759.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1747..3090)
                     /gene="astB"
                     /locus_tag="ACHUN9_002388"
     CDS             complement(1747..3090)
                     /gene="astB"
                     /locus_tag="ACHUN9_002388"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0695760.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3087..4565)
                     /gene="astD"
                     /locus_tag="ACHUN9_002389"
                     /pseudo
     CDS             complement(3087..4565)
                     /gene="astD"
                     /locus_tag="ACHUN9_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4562..5596)
                     /gene="astA"
                     /locus_tag="ACHUN9_002390"
     CDS             complement(4562..5596)
                     /gene="astA"
                     /locus_tag="ACHUN9_002390"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0695761.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     IYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5593..6813)
                     /gene="astC"
                     /locus_tag="ACHUN9_002391"
     CDS             complement(5593..6813)
                     /gene="astC"
                     /locus_tag="ACHUN9_002391"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0695762.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7259..8065
                     /gene="xthA"
                     /locus_tag="ACHUN9_002392"
     CDS             7259..8065
                     /gene="xthA"
                     /locus_tag="ACHUN9_002392"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0695763.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8232..8942
                     /locus_tag="ACHUN9_002393"
     CDS             8232..8942
                     /locus_tag="ACHUN9_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0695764.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8947..9624
                     /gene="ydjY"
                     /locus_tag="ACHUN9_002394"
     CDS             8947..9624
                     /gene="ydjY"
                     /locus_tag="ACHUN9_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0695765.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9639..10346
                     /locus_tag="ACHUN9_002395"
     CDS             9639..10346
                     /locus_tag="ACHUN9_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0695766.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10346..10894
                     /locus_tag="ACHUN9_002396"
     CDS             10346..10894
                     /locus_tag="ACHUN9_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0695767.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10904..12070
                     /locus_tag="ACHUN9_002397"
     CDS             10904..12070
                     /locus_tag="ACHUN9_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0695768.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12043..13579
                     /locus_tag="ACHUN9_002398"
                     /pseudo
     CDS             12043..13579
                     /locus_tag="ACHUN9_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13579..14232
                     /locus_tag="ACHUN9_002399"
     CDS             13579..14232
                     /locus_tag="ACHUN9_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0695769.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14299..15606
                     /gene="ynjE"
                     /locus_tag="ACHUN9_002400"
     CDS             14299..15606
                     /gene="ynjE"
                     /locus_tag="ACHUN9_002400"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0695770.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15615..>15964)
                     /locus_tag="ACHUN9_002401"
     CDS             complement(15615..>15964)
                     /locus_tag="ACHUN9_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0695771.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3699 a         4344 c         4165 g         3756 t
ORIGIN      
        1 tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc
       61 cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg tgcgccagca tgttagcttt
      121 gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct
      181 ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg
      241 ctggcctttc atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt
      301 gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg tgcatcatcg gcttcgcgtc
      361 agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag
      421 ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta
      481 tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag
      541 cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat
      601 tatggagaaa aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacaatgatt
      661 ttgcagagat tatgaagaac tataccggat gactggtgat aaataaagca aataaccagg
      721 attaatctgt attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt
      781 aagtattagc ttattttttc gagcattaat cccgcgcgta atcccaacgc taccaacgga
      841 ttagggaata acacatactc tacatcatgg gttacggtaa aacgttcctc tccgtcctgc
      901 gccagcaatg ttcctttctc aaacggcata aaattcagcg tgtcacttgc catatgcatt
      961 tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggtgtt
     1021 ctcacgatac cgacactctc accagatagc agcgcagcaa ttgcgctggc agttactgca
     1081 aactggcgaa gatcgttttg cccaaagggc aacgctttgc caagttccag cgtacaggcc
     1141 agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac caccaggctc ctgatggaac
     1201 accagcgcct ccagccccgc cgcacccagc cacgtcagaa atttctcgtc ccagggaatg
     1261 tcgcgttgcg gtaatacacc gaactgagga tgcaaggagc cacgaattgc ggtatgtaga
     1321 tcaaggtgcc aacgcacaga ttctttgccc tggtcataaa aatcttccag gcactgttcc
     1381 agttcgcgcg cccgacaggt ttctccgctt tcagcaaata gctgccaacg accgccaaac
     1441 attcggttca tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggatccccg
     1501 aggatcacca gcaaccgcca acgtaacggg atctcgccgt gagatatcgc gccaagcaac
     1561 gcgtccagca tttccacagg tgccgtctca ttaccgtgga tccccgcaga aatcaccagc
     1621 gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagcgccag
     1681 cgaacgccgt tgatttctcg ctcggtgata accggttttt tacccgttaa ggtcagagca
     1741 agaaaattat ccattgcccc ctccctcgcg ctggaacgga taaaccgaac cgagattcag
     1801 taattggctc aatacatcca gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag
     1861 atcggcagca gtaaggcgat cgcggtagta acgatccacc cagtcattga gcgcattaaa
     1921 cagcgtatcg ttcatcatca ccgccggatt caccgcccgg cgttcttctt ctgtcaatac
     1981 tacccgcaac cgcaggcacg ccgggccgcc gccattcgcc atgctttcac gtaaatcaaa
     2041 gacttttagt tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat aaccccatac
     2101 tccggcgtgt tcccgacact cctgaggcag caccaacatc atggaaccat catcgcggct
     2161 caacagttgg ctgttaaaca gataggtaga caccgcatcc gacacggaaa cctgagttgc
     2221 cggaacttct atcgccataa aaccgttcac ccgcgcccgc aggtttgcca gtaactgtga
     2281 ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg cggttactta cggcaatcac
     2341 gtcattatga aaaacaccct ggtcgataac gtccgggttt tgctgggcga aaatcacctg
     2401 ttggggattc acctgattaa gccttgccac cgcctcgctg gcttcgcgag tctgtcgcgc
     2461 cggataacgg gaaggccggg tatcattgcc ttcttctcgc ccgtagacaa aaagttgcat
     2521 acccggctca ccgtaatgac cgccgagacg attgtggttt gccgccccct catcaccgag
     2581 caacgctacc tgcggcaacg ccgaatggac gctaaatttc tcttcgtcgt taaaaatagc
     2641 ttttaacagc gattcagtga cgggcgcttc cagcgaacgg tgaaatttat tgttcaggtt
     2701 ggcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc
     2761 attggctacc cacattggcg aagcggagct gacgctggaa agccagtgcg gtgcctggcg
     2821 tgcaactttt tccagtacct gctcatcgct accactgaat cccaactgac gcagcaccgg
     2881 aataaacgga cgctcgtgcg gcgggatcac ggcctggggg aatcccgcat cggcaagggt
     2941 tttcattttc agtaagccct gtttcgccgc cagtcgcggg ttagacacct gaaaacggtg
     3001 acgggtagag gcttcattac caaacgacag gccagcgtaa tgatgcgtca gccctaccag
     3061 cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct catcggaaaa atccagcccg
     3121 gggttaagcg ttgcgggcaa agttaacgag tccgactcca ggctcgccat aggccatgcg
     3181 cagtaatctg cggcatacca ggcgctgggg cgatggttac cggatgcacc aatgccgccg
     3241 aatggcgcgg tactggcagc accagtaagc ggtttgttcc agttaacaat ccccacccgc
     3301 gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc gcaagagagt
     3361 ccgaagcgag tgttattcgc cattagaatc gcttcctcga aagaatcata acgccagacg
     3421 cgcaataacg gtccgaacac ctcttcatct ggtacgccag caacgcctgt catttcaatg
     3481 atccccggcg tcagcaacga tgtctctgat tgtaataagc gcggcgcaag cagggttcgt
     3541 ccgcccatcg cttccagttg ctgctaggca gtaaccacct gctgtgcggc ctgttcagaa
     3601 atcagcccgc caataaacgg ctgcggttcg tcatcccagt tgcccggcgt taatcgctgg
     3661 ctgacggcaa ccagacgagc aagaaacgca tcgccctgtg ctccgctttt gagcaataaa
     3721 cggcgggcgc aggtgcagcg ttgcccggct gtgacaaacg ccgactgaat ggtcagatgg
     3781 acagccgagt cgatatccgc cacctcatcg ataattagcg gattattacc gcccatctca
     3841 agggcgagaa ttttctccgg ctgaccggag agctggcgat gcagctggta gccggtattg
     3901 gcgctaccgg taaacagcaa accgtcgaga tcctccagcg cactcagcgc ctgacccgtt
     3961 tcacgcccgc cctgcaccag gttcagcacg cccggcggca agccagcctg ctgccataaa
     4021 cgcattaccg cttcgccact ccacggtgtc agttcgctgg gtttaaagat aatggtgtta
     4081 cctgccagca atgccggaac gatatgtccg ttcggcaaat gaccagggaa attatacggc
     4141 ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcacc gtccggcatt
     4201 tcactacgct gctcgccggt acgaacgtga tacgccttaa ttgatatcgc gattttattg
     4261 atcatcgccg tcacttcggt tgccgcttcc cagcgcggct tacccgtttc tctggcaata
     4321 atcgcggtta attcggcttt attgctttcc agcaatccgg caaagcgttc gacaacgacc
     4381 tgacgttcag ccaatgagag ccgcgcccag cgcggaaacg ccgcacgggc tgcccgacaa
     4441 gcctgttcga cctgagcggc atcggcatca ttgccttgcc ataacacctc gcccgatacc
     4501 ggattacgct tcacacgcga tgcgccctgg cccgttatcc agtcaccgtt aatccataaa
     4561 gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc
     4621 atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac
     4681 gcgccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag gccggaaaat
     4741 cgccctgcgc aggctgccct tctgccactt ccaccagccg acttttacgg atggcgcgca
     4801 cgcggtcgat gtcacactca agcgttggcc cgccgtcgaa gatgtcgata tagttgcggt
     4861 aacgaaaacc ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga tgtacctgac
     4921 cgatgacgtc ctgggcttcc tgggataaaa agtgggtata gatcggatgt ttcggcatca
     4981 gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcgacgcgg ctaaaatcca
     5041 tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt tcgtcaatca
     5101 ccccgcgcat ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc gccataaaca
     5161 taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccagtcc gggtcgagaa
     5221 acagcgtgca cagctcgctg ctgccggtgt gatcgttact aagaaacagc gtcggcaatg
     5281 cgttatagat attcagctct tttgaggcgt gaaccaacgt gccgacccga tagttgtacc
     5341 agggatcgtt cagcccaacc gccacctcaa tggcgcaaat ccccgccacg gtgcctgtct
     5401 cgctatcttc cagcacgaac acatagccct gctcgctttt gggcagttcg ccttgccagg
     5461 ttttgattgc cctttcgata cgcgcggaaa gcgtggcttc attggcggga agcgacgtca
     5521 ggccgccgcc cgttttgctg gcaagctgca tcagcgccga gacatctgag cgctcaacgg
     5581 gacggatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcag ctgcaaagcg
     5641 atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa aacgcaccac
     5701 gttgccaccc gcaatcagta ccataacgcc tgctttcgcc gcctcctgag agatctgttt
     5761 cgcttgtccg gcgtaatcgg cattcagtac acagccaatc aacaaaccta agccgcgaac
     5821 ttcactgaac aaaccgtagc ggtgattaat ggtattaaga cgctcaacaa accagtcgtg
     5881 acgctgttta acgccattaa gcatctctgg tgtgttgatg agctccagca ctttgcccgc
     5941 caccgccgag gccagcggat tgccgccata ggtggtgcca tgagtgccaa cggtcatcac
     6001 gctggcgcac tcttcggttg ttaacaacgc accgaccggg aaaccgccgc ccagcgcttt
     6061 ggcggtagtt aacagatcag gtgtcacgcc gtagtgcata taggcataca gttccccggt
     6121 gcgcccgacg ccggtttgta cttcatcaaa aatcaacagc gcattgtggc ggtcacacaa
     6181 ttcacgcaga ccttgtaaaa acgcgttgct ggctggcacc acaccgcctt ccccctggat
     6241 gggttcgaca atcaccgcac aggtggcgtc gtcaatcagc gcgctggcag agttaatatc
     6301 gttatatgca gcatgacgga tatctggtgg cagtggcgca aaatcctgtg aataggctgg
     6361 ctgcccaccc gcactgacag taaacagcgt gcgaccatga aacgcatttt taaacgccac
     6421 gataccgctc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc
     6481 cgcttcgttg gcttccgcac cggagttaca aaagaagacg cgatcggcaa acgtggcgtc
     6541 gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc cggtatgcca
     6601 gaacttactc gcctgttcgt tcagcgcttc acgcagttcc ggatgcgcat ggcccagcgc
     6661 gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat cccacaagcg
     6721 cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg taaacaggta tcatccattc
     6781 atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa ataattcgct
     6841 atctatatgt taacaataag taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc
     6901 agccagtgat aaaagtgcat aaacggcgga ggctaactgg aaatcaagga gttataacca
     6961 aaccatatgc atttaaagtg catataaagt gaatacgttt gcgatgtggg tgaataaaaa
     7021 gaataaaaaa cgcaatgtta tgcagaagta aaatataatt ctggaattgt gatcattgac
     7081 gaaatttact ggaaattact gcgccattct gacgcagcgc gcaccaaaag cgggcatttt
     7141 ttgcgccatc gttgacatca ttaacaacca tcgatcaaat cacttaacaa caggcggtaa
     7201 gcaacgcgaa attctgctac catccacgca ctctttatct gaataaatgg cagcgactat
     7261 gaaatttgtc tcttttaata tcaacggcct gcgcgccaga cctcaccagc ttgaagccat
     7321 cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag acaaaagttc atgacgatat
     7381 gtttccgctc gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg
     7441 ccattatggc gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc
     7501 cggtgacgac gaagaggcgc agcggcggat tattatggcg gaaatcccct cactgctggg
     7561 taatgtcacc gtgatcaacg gttacttccc gcagggtgaa agccgcgacc atccgataaa
     7621 attcccggca aaagcgcagt tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa
     7681 acgtgataat ccggtactga ttatgggcga tatgaatatc agccctacag atctggatat
     7741 cggcattggc gaagaaaacc gtaagcgctg gctgcgtacc ggtaaatgct ctttcctgcc
     7801 ggaagagcgc gaatggatgg acaggctgat gagctggggg ttggtcgata ccttccgcca
     7861 tgctaatccg caaacagcag atcgtttctc atggtttgat taccgctcaa aaggttttga
     7921 cgataaccgt ggtctgcgca tcgacctgct gctcgccagc caaccgctgg cagaatgttg
     7981 cgtagaaacc ggcatcgact atgaaattcg cagcatggaa aaaccgtccg atcacgcccc
     8041 cgtctgggcg accttccgcc gctaatttag cagctctcct ggctcaaact gggtcaggag
     8101 aattaacctt gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg
     8161 ttataccgtc actgcgtttt tagttgtctg accacttctc tattatcaag tttgatatag
     8221 gaaactccac gatgaatgct gagcgtaaat ttcttttcgc ctgtcttatt tttgcgctgg
     8281 tcatttacgc tatccacgct ttcggtttat tcgatctgct caccgattta ccccacttac
     8341 agacactcat tcgccagagc ggattgttcg gttatagcct ctatattctg ttattcatca
     8401 ttgccaccct ctttgtgtta ccaggaagca tactggtgat cgctggagga atagtttttg
     8461 gcccgctctt agggacacta ctctcattaa ttgccgccac gctggcctcc tcgtgctcat
     8521 tcctgctggc gcggtggctg ggacgtgatt tactgctgaa atacgtgggc catagccata
     8581 ccttccaggc cattgaaaaa ggcattgcgc gtaacggtat tgattttctt attctgaccc
     8641 gcttaatccc gttgtttcct tacaatattc aaaattacgc ttacggatta accaccatcg
     8701 ccttctggcc ttataccctt atttcggcac tcacgaccct gcccggtatt gttatttata
     8761 ccgtgatggc aagcgatctc gccaatgaag gcattacgct gcgctttatt ttacaactct
     8821 gtctggcggg cctggcgctg tttattctcg tccagctcgc aaaactctac gcccgacaca
     8881 aacatgtgga tctgtctgct tcgcgccgca gcccgcttac tcacccaaaa aatgaaggat
     8941 agaacgatgt cgcaacatta ttcagtgtca tggaaaaaag gactggctgc actctgctta
     9001 ctggctgttg ccgggcttag cggctgcgat caaaaagaga atgcggcggc aaaagtggaa
     9061 tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg ccaataacca tacggtaacc
     9121 atgttggtgc aaattaatgg tcgtttcctc accgacgaca ctcgtcacgg tattgtgttt
     9181 aaagatggct ccaacggaca taaatcgctg tttatggctt atgcgacccc gaaagcattt
     9241 tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa acatgacgat ggataataaa
     9301 gaaacgactc atgtcacagg cagcaaactg gatatttcgg tcaactggca aggggcggca
     9361 aaagcgtatt ccttcgatga agtgattgtt gacagtaatg gcaagaaact ggacatgcgc
     9421 tttggcggta atttaacggc agcagaagag aagaaaacgg gttgcctggt gtgtctggat
     9481 agctgcccgg tcggcatcgt cagcaatgca acatacactt atggtgcggt ggaaaaacgc
     9541 ggcgaagtga aattcaaagg caatgcctca gttctcccgg cggataacac gctggcaacg
     9601 gttaccttta aaatcaccga ataaagccag gataaaggat gatgaagatg caatcgcgta
     9661 aaatctggta ctaccgtata accctcatca tcctgttgtt cgctatgctg ctcgcatggg
     9721 cgctgcttcc cggcgtccat gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg
     9781 accaacaggg tatagaacgc tttattcagt cttacggcgc actggcggca gttgtctcgt
     9841 tcttgttgat gattttacag gccattgctg caccgctacc tgcgtttttg atcacctttg
     9901 ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct gtcgtggacc agttcgatgg
     9961 ccggcgcggc gctgtgcttt tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat
    10021 taaccggcaa aaccgtgctt gacagtatgg acggcttttt cactcgctac ggcaaacaca
    10081 ccatcctggt ctgtcggtta ttgccttttg tccctttcga tccaatcagc tatgctgccg
    10141 atctgacttc aatacgtttt cgctcgtttt ttatcgccac cgggcttggt caattaccga
    10201 cgactattgt ttattcctgg gcgggcagca tgttaacagg cggtactttc tggtttgtca
    10261 ccggactgtt tattctgttt gccctgaccg tggtgatttt tatggcgaag aaaatatggc
    10321 ttgagcgcca gaagaggaat gactgatggg tttaccgccg cttagcaaaa ttcctttcat
    10381 tttacgtccg caggcgtggc tgcatcgtcg ccattacggc gaggtgctaa gcccgattcg
    10441 ctggtggggg cggatcccgt ttatctttta tttggtgtca atgtttgttg ggtggctgga
    10501 gcgcaaacgc tcaccgctcg atccggtagt gcgatcgctt gtcagcgcgc gcattgcgca
    10561 aatgtgcctg tgtgagtttt gtgtggatat caccagtatg aaagtcgccg agcgtaccgg
    10621 cagcagcgat aaactgctgg cagtggctga ctggcggcaa agcccgctct ttagcgatga
    10681 agaacggctg gcgctggagt acgccgaagc cgcaagcgtc acaccgccaa cagtcgatga
    10741 tgccctgcgt acccgactgg ctgcgcattt tgacgctcag gcgctcaccg aactgacggc
    10801 attgatcggc ctgcaaaacc tgtcggctcg ttttaattct gccatggaca ttcccgctca
    10861 ggggctgtgc cgtattccgg aaaaacgttc ttaaggagag aagatgcgcc attgtgggtg
    10921 gttgctggga ttgttatcgc tgttttcttt ggcaacacat gccagtgact ggcaggaaat
    10981 taaaaatgag gccaaagggc aaaccgtctg gtttaacgcc tggggcggcg ataccgcaat
    11041 taaccgctat ctcgactggg ttagcggcga gatgaaaacc cattacgcta taaacctgaa
    11101 gattgttcgt ctcgcggatg ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc
    11161 cggtcgtaaa acgggcggct cggtggatct gctctgggtg aacggcgaaa acttccgcac
    11221 cttaaaagag gccaatttat tacaaacggg ctgggcggag actctgccca actggcgcta
    11281 tgtcgacaca cagctgccgg tgcgggaaga tttttcagtg ccgacacaag gtgcggaatc
    11341 gccctggggc ggcgcacaac tgacgtttat cgcccgccgc gatgttacgc cacagccacc
    11401 acaaacgccg caagccttac tggagtttgc taaagccaat cccggcacgg ttacttatcc
    11461 gcgcccaccg gactttaccg gcacggcgtt tcttgaacag ttgctgatta tgctgacgcc
    11521 agatcccgcc gcattaaaag aagcgccgga cgatgcgact ttcgcccgtg tcactgcgcc
    11581 cttgtggcaa tatcttgatg cactgcatcc gtatttgtgg cgcgaaggaa aggatttccc
    11641 gccttcgccc gcgcggatgg atgctctgct gaaagccggc acattgcgcc tgtcgctgac
    11701 ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc gatttgccgg caagcagtta
    11761 cagttttggc tttcgcgagg ggatgattgg caacgtgcat ttcgtcacca ttccagccaa
    11821 cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc ctgctctcac ccgatgcgca
    11881 actgcgtaaa gcagatcccg ttgtctgggg cgatccttct gttctcgatc cgcaaaaact
    11941 gcctgacggg cagcgcgaaa tattgcaatc aagaatgccg caggatctgc cgccggtact
    12001 ggctgaaccg cacgcaggat gggtgaatgc actggaacaa gaatggttac gccgttacgg
    12061 tacgcattaa tctttttgct gtgggcgatg gtggcggtga tttatgcacc gctgatcccg
    12121 gcagctctca cgctgatatc gcctgcgttg tcgttgacac actggcaggc gttatttgcc
    12181 gatccacagt taccgcatgc attactggca acgctggtat cgacaaccat cgcggcggtc
    12241 ggggcattgt tgattgccct gctggtgatt gtggcgctat ggcctgggcc gaaatggcag
    12301 cgtatgtgcg cccgtctgcc gtggctgctc gccattcccc atgtggcttt tgccaccagc
    12361 gccctgctgc tctttgctga cggagggctg ctatatgact atttcccgta tttcactccg
    12421 ccaatggacc gatttggcat cggtctgggc ctcacccttg cagtgaaaga aagcgcattt
    12481 ctgctgtgga tcttagcggc agtattgagc gaaaaacggc tgttgcagca ggtcattgtg
    12541 ctggattcgc tgggctacag ccgctggcaa tgcctgaact ggctgctgtt gccctccgtc
    12601 gcgccttcgc tggcaatggc gatgctggcg attgttgcct ggtcgctttc ggtcgtggat
    12661 gtggcgatta ttcttgggcc aggtaatcca ccgacgctgg cagtaattag ctggcagtgg
    12721 ttaactcagg gcgacgcgga tcaacaaacg aaaggcgcac tcgccagtct gctgctgatg
    12781 ctgttactcg ccgcctacgt tttgctgagc tatctgctat ggcgcagctg gcggcgcact
    12841 attccccgcg tagatggcat tcgcaatgcc tgccacacct ttattaccag gcactacgct
    12901 ggcgagtttt ttacccttaa ccggtgtgct gtgtgtggtt ctgctggcga tcctcgcgga
    12961 tcagtcgacg atcaatagtg aagcactcat caacagtctg acaatggggc tggtagcaac
    13021 attcatcgct ttgctcctgt tactgctgtg gctggaatgg gggccacagc gtcgccagtt
    13081 gtggctatgg ttgcccattc tattgcccga tctgccactg gtggcgggcc agtacacgct
    13141 ggcgctatgg ctgaacctag atggaagctg gacggcggtg gtctgggggc atctgctgtg
    13201 ggtaatgccg tggatgctgt ttatcctgca acccgcctgg cagcgcattg attcacggtt
    13261 aattttgatt gcgcaaacac tgggctggtc gcgggccaaa atcttctttt acgtgaaatg
    13321 cccactcatg ttgcgccctg tgctgattgc cttcgcggtg ggattttcag tcagtattgc
    13381 gcagtatatg ccgacgctgt ggctgggcgc ggggcgtttt ccgacgctca ccactgaggc
    13441 ggtggcatta agcagcggcg gcagcaacgg tattctcgcc gcccaggctt tatggcaact
    13501 gttgttaccg cttattattt ttgccctgac cgcgttagtc gcaaaatggg taggttatgt
    13561 cagacaagga ctccgctaat gctctgcgtg aaaaatgttt cgctacgttt accagaaagc
    13621 cgcttgctga caaacgttaa ctttacggtt gataaaggtg acatcgtcac gttaatgggg
    13681 ccgtctggct gtggaaaatc cactctgttt tcatggatga ttggtgcact ggctggacag
    13741 ttttcttgta caggtgagct atggctcaat gagcaacgga ttgacatgct acccaccgca
    13801 cagcgtcaga ttggcattct tttccaggat gcactgttat ttgaccagtt cagcgtcggg
    13861 caaaatttac tgctggcgct actgtcgacg ctcaagggga ctgccaggcg caatgccgtg
    13921 aaagacgcgc ttgatcgagc gggactggct gaaacttatc accaggaccc tgcgacatta
    13981 tcaggcggtc agcgagcgcg ggtggccctg ctgcgcgccc ttctcgctca gccaaaagcg
    14041 ttactcctgg atgagccatt cagccgtctt gatgtggctc tgcgcgataa ttttcgccag
    14101 tgggtgttca gcgaagttcg cgaactggcg atccccgtcg ttcaggtaac gcacgatctc
    14161 caggatgttc ctgctgatag ttctgttctg gatatggcgc agtggtcaga aaattacaac
    14221 aaactgcgat aacgcaaagt ttttctcaat gcgtcagttc agaatggcgc actcaaaact
    14281 acaatgtcgg gattttcgat gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta
    14341 gggctggctt gcgcttcttc gtgggccgct gaactggcga agcctcttac acttgaccag
    14401 cttcaacaac aaaatggcaa agcgatagat actcgcccca gcgcgtttta taacggctgg
    14461 ccacaaacct taaatggccc ttctggtcat gaacctgccg ccttaaacct ctctgccagc
    14521 tggctcgaca aaatgagcac cgaacagctc aacgcgtgga tcaagcaaca taacctgaaa
    14581 gccgatgccc cggtggcgct gtacggtaat gacaaagatg tcgacgccgt caaaacgcga
    14641 ctgcaaaaag caggttttac gcatatctcc attctgagtg acgcgctaag cgaaccttcc
    14701 cgtctacaaa aactgccgca ctttgagcag ctggtttatc cgcaatggct gcacgacctg
    14761 caacaaggta aagaggttac ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc
    14821 tggggcgctc ctaagcttta ccttatcagc catattcccg gtgctgacta catcgatacc
    14881 aacgaagtgg aaagcgaacc gctgtggaac aaagtttctg atgaacaact aaaagcgatg
    14941 ctggcaaaac acggcattcg ccatgacacc acggtcattc tgtacgggcg tgacgtatac
    15001 gctggagcgc gtgtggcgca aattatgctc tacgcaggcg tgaaagatgt gcgtctgctg
    15061 gatggcggct ggcaaacctg gtccgacgcg ggactgcctg ttgagcgagg aacgccaccg
    15121 aaagtgaaag cggaaccgga tttcggcgtg aagatcccgg cacaaccgca gttgatgctc
    15181 gatatggaac aggcccgtgg actgctgcat cgccaggatg catcgctggt gagcattcgt
    15241 tcgtggccag aatttatcgg tacgaccagc ggttacagct atattaaacc aaaaggtgaa
    15301 atagccggag cacgttgggg acacgctggt agcgactcga cgcatatgga agattttcat
    15361 aacccggatg gcaccatgcg cagcgccgat gatattaccg ctatgtggaa agcgtggaat
    15421 atcaaaccag agcagcaagt ttcattctac tgcggcaccg gctggcgcgc gtccgaaacc
    15481 tttatgtacg cacgcgccat gggttggaag aatgtttccg tgtatgacgg cggctggtac
    15541 gaatggagca gcgatccaaa aaatccggta gcaaccggtg aacgcggccc ggacagtagc
    15601 aaataacatt gcaattactg acgctggagc gacttcagcg tcagataccc actccacacc
    15661 cgcgtaaagg tcgtcatcca gcacaacgca ccgaagatcc acgcaaacca cgcaaaccat
    15721 gcagggaata aacatcccag cacaaacagt aagatcgttt ctgtgccttc ggttaatccc
    15781 cccagatagt aaaacgactt atgtgcataa ccggggttat caatctgatg ttttgctgcc
    15841 agtgcagcaa aggcgagaaa actgctgccc gtgccgataa acgcaaacaa caaccagccg
    15901 cctgccagcg cattttgctc cggtgcagca agaataaagc caaacggcac cagcgcgtaa
    15961 aaga
//
LOCUS       DAZOYS010000087        15789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOYS010000087 DAZOYS010000000
VERSION     DAZOYS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.087"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHUN9_002402"
     CDS             <1..578
                     /locus_tag="ACHUN9_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0695772.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHUN9_002403"
     CDS             581..1744
                     /locus_tag="ACHUN9_002403"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0695773.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHUN9_002404"
     CDS             1828..3453
                     /locus_tag="ACHUN9_002404"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0695774.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUN9_002405"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUN9_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0695775.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUN9_002406"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUN9_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0695776.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUN9_002407"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUN9_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0695777.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUN9_002408"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUN9_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0695778.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUN9_002409"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUN9_002409"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0695779.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUN9_002410"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUN9_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUN9_002411"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUN9_002411"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0695780.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHUN9_002412"
     CDS             9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHUN9_002412"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0695781.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9737..10382
                     /gene="ulaD"
                     /locus_tag="ACHUN9_002413"
                     /pseudo
     CDS             9737..10382
                     /gene="ulaD"
                     /locus_tag="ACHUN9_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            10392..11246
                     /gene="ulaE"
                     /locus_tag="ACHUN9_002414"
     CDS             10392..11246
                     /gene="ulaE"
                     /locus_tag="ACHUN9_002414"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0695782.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11246..11932
                     /gene="ulaF"
                     /locus_tag="ACHUN9_002415"
     CDS             11246..11932
                     /gene="ulaF"
                     /locus_tag="ACHUN9_002415"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0695783.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12029..12580
                     /locus_tag="ACHUN9_002416"
     CDS             12029..12580
                     /locus_tag="ACHUN9_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0695784.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12655..12930)
                     /gene="yjfY"
                     /locus_tag="ACHUN9_002417"
     CDS             complement(12655..12930)
                     /gene="yjfY"
                     /locus_tag="ACHUN9_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR0695785.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13257..13652
                     /gene="rpsF"
                     /locus_tag="ACHUN9_002418"
     CDS             13257..13652
                     /gene="rpsF"
                     /locus_tag="ACHUN9_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0695786.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13659..13973
                     /gene="priB"
                     /locus_tag="ACHUN9_002419"
     CDS             13659..13973
                     /gene="priB"
                     /locus_tag="ACHUN9_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0695787.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13978..14205
                     /gene="rpsR"
                     /locus_tag="ACHUN9_002420"
     CDS             13978..14205
                     /gene="rpsR"
                     /locus_tag="ACHUN9_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0695788.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14247..14696
                     /gene="rplI"
                     /locus_tag="ACHUN9_002421"
     CDS             14247..14696
                     /gene="rplI"
                     /locus_tag="ACHUN9_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0695789.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14808..15779)
                     /locus_tag="ACHUN9_002422"
     CDS             complement(14808..15779)
                     /locus_tag="ACHUN9_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0695790.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
BASE COUNT         3664 a         3873 c         4220 g         4032 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     9421 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     9481 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     9541 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     9601 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     9661 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     9721 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     9781 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     9841 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     9901 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     9961 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
    10021 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
    10081 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
    10141 tcaccgcagc cgtgacgcgc aggccgctgg cgtggggtga agccgatatc actgcgatca
    10201 aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg tggcctggcg ctggaagatc
    10261 tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc cggtcgcagc atccgtgatg
    10321 ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc catcgccgaa ctgtggggct
    10381 aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat ctatgaaaaa gcgctccccg
    10441 ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac gttgggcttc gattttgtcg
    10501 agatgtcggt agatgaaact gacgagcgcc tgtcgcgcct cgactggagc cgcgagcagc
    10561 gtctggcgct ggtgaatgcg attgttgaaa ccggcgtgcg tgtgccgtcc atgtgccttt
    10621 ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc ggtgcgggcg caggggctgg
    10681 agattatgcg taaagccatc cagttcgccc aggacgttgg cattcgcgtg atccagctgg
    10741 cgggctatga cgtttactat caggaagcca ataacgaaac gcgtcgtcgt ttccgtgacg
    10801 gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt gacgctggcg atggagatca
    10861 tggattatcc gttgatgaac tccatcagca aggcgctggg ttacgcgcac tatctcaaca
    10921 acccgtggtt ccagctctac ccggatatcg gcaacctgtc ggcgtgggac aacgatgtgc
    10981 agatggagtt gcaggccgga atcgggcata tcgtcgcggt gcatgtgaaa gacaccaaac
    11041 cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt ggatttcgaa cgttgcttcg
    11101 aaacgctcaa acagagtggc tattgcgggc cgtacctgat tgagatgtgg agcgaaacgg
    11161 cggaagaccc ggcggcagaa gtggtgaaag cgcgtgattg ggtgaaagcg cgcatggcca
    11221 aagcaggcac ggtggaggcg gcataatgca aaagctaaaa cagcaggtgt ttgaagccaa
    11281 catggatctg ccgcgctatg ggctggtgac ctttacctgg ggcaacgtca gcgctatcga
    11341 ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt gcctacgaaa ccatgaaagc
    11401 agacgatatg gtggtggtcg atatgagcgg caaggtggtg gaaggggagt atcgcccgtc
    11461 ttccgacacc gcgacgcatc tcgaactcta ccgtcgttac ccgtcgcttg gcggcattgt
    11521 ccatacccac tccactcatg ctactgcatg ggcgcaggcg gggctggcga tcccggcgtt
    11581 aggcactacg cacgccgact acttctttgg cgacatcccg tgtacgcgcg gattaagcga
    11641 agaagaggtg cagggcgagt atgaactgaa caccggcaaa gtgattatcg aaacgctggg
    11701 tgacgccgag ccgctgcata cgccggggat tgtggtgtat cagcacggtc cgttcgcctg
    11761 ggggaaagat gcccacgacg cggtgcataa cgcggtggtg atggaagaag tggcgaaaat
    11821 ggcgtggatt gcgcgcagta ttaacccaca actgaatcac atcgacagct tcctcatgaa
    11881 taaacacttc atgcgtaagc acggtcctaa cgcctattac gggcagaagt aagcagtgcg
    11941 ccccggaatt attacctcct gcgggatgta attggtctga ttccggggcc gcttcagtta
    12001 aaaaatcgta taaacaacag gtgtttttat ggctatcaat ggattgtttg tcagaacgga
    12061 tccgcaacgt cgccgctatg gcgttgcgtt acttgtgggt gtactttcag gaattatttc
    12121 agcatttgtt aaatggggag cggaagtccc cttgccaccg cgcacatttt ccggtggtcg
    12181 cgatgaattt aacccgccgt atatctttct acgggattat ttaggtatcg acccaaccca
    12241 aactgtgtat acattctcgg aacatattat caacccggtg atggtgacgc atatcatctt
    12301 ctcactggtt tttgcgatag ggtattgtat tgtcgcggaa attttcccga aagtgaaatt
    12361 atggcagggg atattagcag ggcttattgt gacagtggca gtccacggta tattttgccc
    12421 ggcgctgaat ttaacgccgc cgctcactca attgcctttt gacgaatatg cctctgaaat
    12481 cctggggcat attttctggt tctggataat agaaattatg cgccgcgact tacgtaatcg
    12541 cattactcat gagccagatc cggaagtcgc aatccggtga ataaccgcat aaaaaagccc
    12601 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
    12661 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac
    12721 gtggtagtac gttgcgccct gcgcatctgt ctcttcattt aatgcctgac gcgcttcgct
    12781 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat catccatgtt
    12841 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
    12901 agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg
    12961 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
    13021 ccatttctga tgcagttgtt caaaaaaaca ccatgatgaa gtgtgatgaa ctccaaatca
    13081 gcggtttaga ggttaattgc gaaaggggag atttatttcg gctctgccct tgagtttagc
    13141 ggggcataca agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
    13201 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
    13261 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
    13321 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
    13381 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
    13441 tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
    13501 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
    13561 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
    13621 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
    13681 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
    13741 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ttttcaccgg caggcgtggt
    13801 gccaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
    13861 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
    13921 gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac tagccatatg
    13981 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
    14041 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
    14101 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
    14161 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
    14221 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
    14281 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
    14341 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
    14401 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
    14461 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
    14521 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
    14581 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgagcac gaagtgagct
    14641 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
    14701 ttattcaacg agacgtaaaa acgccgacca gtggtcggcg ttttgctttt gtagcgagca
    14761 cttgcagata tcgataaggg ccacaataag cagccctacg aagataatta cttagctgcc
    14821 tgaatatcag ccagcattgg ggcaagggct gggttgtcct tagcgaagtt tgcgtacatt
    14881 ggctgtactt tctcgataaa cggagcttta tctacctggt agactttcac gcccatttta
    14941 tccagttttg caaattcttg tttctcttcc tcgctccaca agtctttcat gtagctcatt
    15001 gattctttag ctgcttttaa tataactgcc tgattttccg ggctaagttt atcaaacgca
    15061 gcattgctca tcagaaccac atcgggaacc atagtgtgtt cgtcaaggct gaaggatttt
    15121 gccacttcac cgtgacgcat ggtggtcagc gccatcacgc tgttttctgc catatcgacc
    15181 acaccctgct gtaaggctgt atacaactcg ccgtaatcca gtggcgtcgg gttaccgccc
    15241 atgactttga tcatttcaac tgcgccaggg cttggctgga cacggacttt catgcctttc
    15301 agatcgtctg gtttcagtac tggtttattg ccatagaaac tgcgggcgcc tccatcataa
    15361 aaagtcagcc cgaaataacc tttgcttttc gttgagtcga ggatttttct cccgatatcg
    15421 ccctgcatga cctgataata atgatcacga tcgcggaaca aataaggcag gctaaataat
    15481 ttataggaat tttcaaatgc ttccatttct gccgcattgg tttttaccag tggaatagcg
    15541 ccagaacgaa tcagctccat tgtttctcgt tgagtaccca atgttccatt tggataaata
    15601 cgaatagtaa tatcaccgtt agagtactct ttgttctttt tcgcaaagaa ctccatcgct
    15661 ttatgaacag gatgagactt atcctgatta tgactcagtt ttaatgtcac tgcccaggac
    15721 tggcttgcgg ataacataag aacagcaccg ataatgccag cggatacttt tattttcata
    15781 gatgttcct
//
LOCUS       DAZOYS010000088        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOYS010000088 DAZOYS010000000
VERSION     DAZOYS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.088"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUN9_002423"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUN9_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0695791.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUN9_002424"
     CDS             567..650
                     /locus_tag="ACHUN9_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695792.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUN9_002425"
     CDS             750..1571
                     /locus_tag="ACHUN9_002425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0695793.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUN9_002426"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUN9_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0695794.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUN9_002427"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUN9_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0695795.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUN9_002428"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUN9_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0695796.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUN9_002429"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUN9_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0695797.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUN9_002430"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUN9_002430"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0695798.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUN9_002431"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUN9_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0695799.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUN9_002432"
     CDS             8347..9729
                     /locus_tag="ACHUN9_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0695800.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUN9_002433"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUN9_002433"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0695801.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUN9_002434"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUN9_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0695802.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUN9_002435"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUN9_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0695803.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUN9_002436"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUN9_002436"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0695804.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUN9_002437"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUN9_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0695805.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUN9_002438"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUN9_002438"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0695806.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3812 a         4021 c         3793 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZOYS010000089        15405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOYS010000089 DAZOYS010000000
VERSION     DAZOYS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.089"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..452
                     /locus_tag="ACHUN9_002439"
     CDS             <1..452
                     /locus_tag="ACHUN9_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0695807.1"
                     /translation="LCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISL
                     PTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPP
                     FHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            668..757
                     /locus_tag="ACHUN9_002440"
                     /pseudo
     CDS             668..757
                     /locus_tag="ACHUN9_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHUN9_002441"
     CDS             complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHUN9_002441"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0695808.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHUN9_002442"
     CDS             complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHUN9_002442"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0695809.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHUN9_002443"
     CDS             complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHUN9_002443"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0695810.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHUN9_002444"
     CDS             complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHUN9_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0695811.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3725..4513
                     /gene="modE"
                     /locus_tag="ACHUN9_002445"
     CDS             3725..4513
                     /gene="modE"
                     /locus_tag="ACHUN9_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0695812.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4581..6053
                     /gene="modF"
                     /locus_tag="ACHUN9_002446"
     CDS             4581..6053
                     /gene="modF"
                     /locus_tag="ACHUN9_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0695813.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6314..7330
                     /gene="galE"
                     /locus_tag="ACHUN9_002447"
     CDS             6314..7330
                     /gene="galE"
                     /locus_tag="ACHUN9_002447"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0695814.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7340..8386
                     /gene="galT"
                     /locus_tag="ACHUN9_002448"
     CDS             7340..8386
                     /gene="galT"
                     /locus_tag="ACHUN9_002448"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0695815.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8390..9538
                     /gene="galK"
                     /locus_tag="ACHUN9_002449"
     CDS             8390..9538
                     /gene="galK"
                     /locus_tag="ACHUN9_002449"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0695816.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9532..10572
                     /gene="galM"
                     /locus_tag="ACHUN9_002450"
     CDS             9532..10572
                     /gene="galM"
                     /locus_tag="ACHUN9_002450"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0695817.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHUN9_002451"
     CDS             10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHUN9_002451"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0695818.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHUN9_002452"
     CDS             complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHUN9_002452"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0695819.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13054..13434
                     /locus_tag="ACHUN9_002453"
     CDS             13054..13434
                     /locus_tag="ACHUN9_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0695820.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13548..14489
                     /gene="zitB"
                     /locus_tag="ACHUN9_002454"
     CDS             13548..14489
                     /gene="zitB"
                     /locus_tag="ACHUN9_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0695821.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHUN9_002455"
     CDS             complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHUN9_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0695822.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15243..>15405)
                     /locus_tag="ACHUN9_002456"
     CDS             complement(15243..>15405)
                     /locus_tag="ACHUN9_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0695823.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4043 c         3850 g         3596 t
ORIGIN      
        1 gcctttgcat ggatcttgca ctggtaacag gacagcgcga aggagatctg gcggctatga
       61 gatgggagga tataagagat ggcaggctat acgtggaaca acaaaaaaca ggggcaaaaa
      121 ttcgcatttc tctccccacc acgatttcca gattgaattt aacacttgcc gatgtactgg
      181 ataacttgaa aaaaataaac ggtaaaaacg aaaagttact cggagggaag acagccagga
      241 caatcgcagc acagttcagg attgcccgag acacatccgg attgaaatgg gaaggtgacc
      301 caccgccatt tcatgaaatc cgaagtttat ccggtcgcct gcactcagca gaaaaaggcg
      361 gtgatttcac tcaggcgctc cttggacatc gttcatccag catgacggat aaataccgtg
      421 acggacgagg gagggaatgg aaagacattt agaattaatt tcgaatgatt tcgaaacacc
      481 acttgtaatt aattgattat aaatgattaa ttcctaatgg gcatttgctt catgaatgct
      541 cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc ggtgaatgct
      601 gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc gcagacgaaa
      661 attcttaaat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
      721 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
      781 taatttgcgc gtacagccac aggccaggtt tgatcgccag ttcatccctg gcccacgggc
      841 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttccacc tggccgttgt
      901 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
      961 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1021 gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1081 aatgcggatg atgctcaagt accgtcactt tcagaatgct actttgttgc tctttcggca
     1141 gccacggatt catcacgcta ctgccccaca cttcctccag cgcgccaaag gctttcacct
     1201 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     1261 tgacatacaa catcggaatg ttgatttccc gtgtcagccg ttgcagataa ggcaacagtt
     1321 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattccggtg
     1381 ctgtcagcaa agcccgacca atcgccacgc gctgtttttc gcctccggac aggctgcctg
     1441 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     1501 ccatactttt cgacatgccg tagcgcagat tgccacgcac tttgtaatgc gggaacagcc
     1561 gcgcatcctg aaaaacatag ccaacgcgac gcttttccgg cgtcaggcag ataccttttt
     1621 cggcatcatt tagtacccgc ccattgagga caatccgccc tttttgcggg cgcgtcagtc
     1681 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     1741 tgccattggc gggcagtgtt tcattaatgg tcaggcaatg gttgcccaac gtctgggaaa
     1801 aattcagctc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     1861 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     1921 actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg ttcgcgtttc
     1981 gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2041 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     2101 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga catcaacccc
     2161 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     2221 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     2281 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     2341 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     2401 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     2461 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     2521 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     2581 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     2641 gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc gtcagaaccg
     2701 taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc acgaacatct
     2761 tccgccggag ccagtttcgg ggagagcgta tcccatgcgc ccagtttttg cagtgcttct
     2821 tttgcataaa tgccagcagg gacatgttcc ggatcgccaa ccgcgaggcg accgccattc
     2881 agcagcgaag tccagttagt tgtgctgtcg atgatgaaat ctttctgctc gctggctttc
     2941 ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt atcgatcgct
     3001 tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag atccgcaggc
     3061 gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga aaccacatcc
     3121 acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     3181 gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac agcgaaagag
     3241 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     3301 cgttatatag gtgacaatat aacggctagc tcagaatttt cccagcagtt attgctaacc
     3361 tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag aaaaagaaat
     3421 gcccgactaa gcgggcattc agggaatcaa tgattttgtc cgggctggtc ttttttacca
     3481 acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag gatgatggcc
     3541 atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc taacatggtc
     3601 aactccagat atagtcatga gactattcta accgctaagc acagaaaagc actccccttt
     3661 tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt gccaggacat
     3721 tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat tcgccgaccc
     3781 gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc agggagcgaa
     3841 agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga atcagttaag
     3901 cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac
     3961 ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc
     4021 ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg ccgcgatctc
     4081 acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga
     4141 tcatgatgac attcaacagc atgttgatgt cttactggct gacggaaaaa cacgcctgaa
     4201 agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca aagaagtgtt
     4261 gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg cacaaaacgc
     4321 cgacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc agtgcgaagt
     4381 attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac
     4441 ttctcttcag caagggcaga atgtcacggc ctactttaat gccgacagcg tgattatcgc
     4501 cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt cagtaatcgc
     4561 tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta
     4621 gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt
     4681 ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc
     4741 cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc tccttcgagc
     4801 aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg ctcggccctg
     4861 gcgaagatga caccggacgc actacggctg aaatcattca ggatgaagta aaggatgcac
     4921 cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac cgacgcttta
     4981 aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc
     5041 ctgacttgtt gattcttgat gagtcgttcg atggcctgga tgttgcctca cgtcagcagc
     5101 tggctgagcg actcgcctcg ttacatcagt ccggtattac tctggtactg gtgctcaatc
     5161 gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat tgcacgttag
     5221 cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa ctggcgcata
     5281 gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca cgtcacgcct
     5341 tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat aacgatcgcc
     5401 ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa attgtcgggc
     5461 caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat ccgcaaggtt
     5521 acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc atctgggata
     5581 tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg gtcagcacta
     5641 ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat caggccgttt
     5701 cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt gataaacgta
     5761 cggctgacgc tccgttccat agtctttcct ggggacagca gcgtctggcg ctgatcgtcc
     5821 gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag gggcttgatc
     5881 cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc
     5941 aattgttatt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc catcgccttg
     6001 agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat tgagtcggta
     6061 gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc gacgcatgca
     6121 ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     6181 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     6241 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     6301 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     6361 ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     6421 taagcgcagc gtactgcctg ttatcgagcg cttaggcggc aaacatccaa cgtttgttga
     6481 aggcgatatt cgtaacgaag cgttgatgac cgagatactg cacgatcacg ctatcgacac
     6541 cgtgatccac ttcgccgggc tgaaagccgt cggcgaatcg gtacaaaaac cgctggaata
     6601 ttacgacaac aatgtcaacg gcactctgcg cctgattagc gccatgcgcg ccgctaacgt
     6661 caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca aaattccata
     6721 cgttgaaagc ttcccgaccg gcacaccgca aagcccttat ggcaaaagca aattgatggt
     6781 ggaacagatc ctcaccgacc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     6841 ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     6901 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     6961 gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt acatccacgt
     7021 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     7081 acacatctac aacctcggtg ctggcgtagg cagcagcgtg ctggacgtgg ttaatgcctt
     7141 cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg aaggcgacct
     7201 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     7261 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     7321 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     7381 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     7441 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag attgcttcct
     7501 ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg ggacttacgt
     7561 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     7621 tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     7681 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
     7741 gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct ttgaaaacaa
     7801 aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
     7861 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
     7921 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
     7981 cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt tcgaaacgct
     8041 actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc agcgcagcga
     8101 tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
     8161 cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc aacactggca
     8221 gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
     8281 tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
     8341 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
     8401 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
     8461 ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
     8521 gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg accgtaaagt
     8581 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca atgcgcccat
     8641 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
     8701 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
     8761 tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
     8821 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
     8881 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
     8941 tcatgccttg ctgattgact gccgctcact ggggactaaa gcagtttcca tgccgaaagg
     9001 tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
     9061 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ctctgcgcga
     9121 tgtcaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg
     9181 cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
     9241 aggcgatctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
     9301 tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag cagtgattgg
     9361 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat
     9421 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
     9481 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
     9541 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
     9601 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc tcgtattccg
     9661 ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag
     9721 gatcaggccg cgtttctggg ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc
     9781 cgttatacct ttgacggtga aacggtgacg ctttcgccaa gtcagggcgt taaccagctg
     9841 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
     9901 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg taatctcggc
     9961 gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    10021 gttgataaat cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    10081 cagtctgacg tgcgcaatca caagttgcag attctggcgg gcgaatatct gccggttgat
    10141 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt tgatttccgc
    10201 agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    10261 tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    10321 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctcccgc tcttcaattc
    10381 tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta cgccgactgg
    10441 caaggactgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg
    10501 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    10561 attgctgagt aatgttaagc cctcgtcatg agggctttat cttcatattg ttcaaatcac
    10621 cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact gcgccactat
    10681 tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat aatgagaatt
    10741 attatcataa aaagataatt tgaggagtaa gtatatggct gtaactaagc tggttctggt
    10801 tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt acgacgtgga
    10861 tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga aagaggaagg
    10921 ttacagcttt gactttgctt acacttctgt gctgaaacgc gctatccata ccctgtggaa
    10981 tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga aactgaacga
    11041 acgtcactac ggtgcgttgc agggtctgaa caaagcggaa accgccgaaa agtatggcga
    11101 cgagcaggtg aaacaatggc gtcgtggttt tgccgtgact ccgccggaac tgactaaaga
    11161 tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa ctgagcgaga aagagctgcc
    11221 gctgactgaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga atgaaactat
    11281 tctaccgcgt atgaagagcg gtgagcgcgt gatcatcgct gcacacggta actctttacg
    11341 tgcgctggtg aaatatcttg ataacatgag tgaagaagag attcttgagc tgaatatccc
    11401 gaccggcgtg ccgctggtgt atgagttcga cgagaatttc aaaccgctga aacgctatta
    11461 tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg gttgctaacc agggtaaagc
    11521 gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc ggcttttttg
    11581 ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg
    11641 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    11701 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc caatgcaggc
    11761 atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct gattgccttc
    11821 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    11881 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    11941 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12001 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcacc
    12061 gttaccgctg gtattcacaa tcgccgaatg cccccattta gttacggaca ggaagcagtg
    12121 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt ctgtgccatt
    12181 tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct gcgattccgt
    12241 ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatactgag gagtgatcat
    12301 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    12361 ggctatacgc agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcaa
    12421 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    12481 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    12541 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    12601 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagttacggg
    12661 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    12721 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    12781 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta
    12841 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    12901 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    12961 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13021 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13081 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    13141 caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc
    13201 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    13261 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    13321 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaatc
    13381 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    13441 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    13501 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    13561 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    13621 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    13681 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    13741 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    13801 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    13861 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    13921 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    13981 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14041 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    14101 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    14161 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    14221 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    14281 ggatggtagg cgagaagccg gtgataacgc tgcatgtgca ggtgatcccg ccacacgatc
    14341 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    14401 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    14461 tgtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct ctttcacgtg
    14521 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    14581 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    14641 cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc acgatagata
    14701 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    14761 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    14821 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    14881 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    14941 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15001 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15061 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    15121 ctaccgcttc gatccatgag agatcataac cgcctgcccc tattggtata tgtaccagga
    15181 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    15241 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    15301 tctcgcagcc tttcatcaag aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg
    15361 atggactgaa ggccattcat ggccatccac ggacaatgcg cgcag
//
LOCUS       DAZOYS010000090        15338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOYS010000090 DAZOYS010000000
VERSION     DAZOYS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.090"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUN9_002457"
     CDS             <1..1015
                     /locus_tag="ACHUN9_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0695824.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUN9_002458"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUN9_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0695825.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUN9_002459"
     CDS             1797..1904
                     /locus_tag="ACHUN9_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0695826.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUN9_002460"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUN9_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0695827.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUN9_002461"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUN9_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0695828.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUN9_002462"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUN9_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0695829.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUN9_002463"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUN9_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0695830.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUN9_002464"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUN9_002464"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0695831.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHUN9_002465"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHUN9_002465"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0695832.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUN9_002466"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUN9_002466"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0695833.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHUN9_002467"
     CDS             complement(11226..11813)
                     /locus_tag="ACHUN9_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0695834.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHUN9_002468"
     CDS             11983..12561
                     /locus_tag="ACHUN9_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0695835.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHUN9_002469"
     CDS             complement(12691..13452)
                     /locus_tag="ACHUN9_002469"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0695836.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUN9_002470"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUN9_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0695837.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUN9_002471"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUN9_002471"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR0695838.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3744 a         4176 c         3837 g         3581 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataa
//
LOCUS       DAZOYS010000091        15231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOYS010000091 DAZOYS010000000
VERSION     DAZOYS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.091"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUN9_002472"
     CDS             267..1925
                     /locus_tag="ACHUN9_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0695839.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUN9_002473"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUN9_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0695840.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUN9_002474"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUN9_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0695841.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUN9_002475"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUN9_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0695842.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUN9_002476"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUN9_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0695843.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUN9_002477"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUN9_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0695844.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUN9_002478"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUN9_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0695845.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUN9_002479"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUN9_002479"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0695846.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUN9_002480"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUN9_002480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHUN9_002481"
     CDS             10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHUN9_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0695847.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHUN9_002482"
     CDS             11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHUN9_002482"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0695848.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHUN9_002483"
     CDS             11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHUN9_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0695849.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            12471..12962
                     /locus_tag="ACHUN9_002484"
     CDS             12471..12962
                     /locus_tag="ACHUN9_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0695850.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHUN9_002485"
     CDS             complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHUN9_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0695851.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPQAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHUN9_002486"
     CDS             complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHUN9_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0695852.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHUN9_002487"
     CDS             complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHUN9_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0695853.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKKDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHUN9_002488"
     CDS             complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHUN9_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0695854.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         3747 a         3549 c         4073 g         3862 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc
     9841 tgtcttgctg gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa caaacttttt
     9901 gaaaaaatcg cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg
     9961 ctttttttgc caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata
    10021 agtgcgaata aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca
    10081 cccaacgttc gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag
    10141 agtcagagca tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc
    10201 tcctgttgaa atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt
    10261 tgattctatt ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact
    10321 ttttcgcctg gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt
    10381 gctgcattgg tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca
    10441 accgctgcct gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact
    10501 gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt gctggagtcg
    10561 aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt
    10621 tctgagccaa tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat
    10681 tattaatcaa caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg
    10741 tggtgttcat gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg
    10801 cgcaatgttg gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca
    10861 tccacgcgtg acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga
    10921 gtacatttct cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga
    10981 ccgcgcacgc ggctactaat aaagcatcag gattcctgct atccttggcg ggaattgaat
    11041 acaggagaga gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc
    11101 atatagcaca ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc
    11161 gatcaccgat catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat
    11221 gcgtatctgg ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat
    11281 taaaaatgtt gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat
    11341 tattgccggt tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc
    11401 gatgatcgcc actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa
    11461 atatgaaata gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga
    11521 aatcaataat tcttcatttt tacactcacg taagggcagt gaagacaact gtcgtgcggt
    11581 agccgcagcg gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc
    11641 gtttaccatg ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc
    11701 agagcgcatt ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat
    11761 ggcaccgatt gcggaatttg cagaccttta attactagta atggaaatat attaatgaac
    11821 gagttttcta tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct
    11881 ttactggtgc cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg
    11941 gagcaggatg agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc
    12001 gattacaatg cgttgtttat cggcgatgaa tgtgcagtgc cgccatatcg tagcgcatgg
    12061 gttgaggacg taacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg
    12121 gcggatacgc cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat
    12181 cagtcaacgg aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc
    12241 tggtgtggtg cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc
    12301 atggcaccgc taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct
    12361 gaagagtaaa tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa
    12421 atgtgtgctc gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac
    12481 ttctctctat tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt
    12541 ccctgggctt actaagctgg gcgggcttct tgggctgtac ggcctacttt gcctgcccgc
    12601 aaggtgggct gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg
    12661 cgatggtcat tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca
    12721 taaccggtat tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg
    12781 ttccggggac gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg
    12841 tgttaccttc gctggcgctt gggctggtat ttggttacgc aatgaaaaac agcggcctgt
    12901 ggctggcggc gcgtagtgca aagacggctc accgtgagca ggaaatcaaa aataaagcgt
    12961 gaggggcact cacgctttcg cttaaacagt aaaatgccgg attattaatt ccggcatttt
    13021 tatctgtcag gattctggtg gaaccgacat atggcggtat ttaaccagaa tgtcattctg
    13081 ccgttctgct ttgttctgca aatcccacag accacggtcg ataccatcat taatcaggaa
    13141 aatgacccct gtttcgatag ccgacatcag acacagcata acgggttcgt ttgaggtgta
    13201 acccacttcc ccttccagca agcgctggta gtcaataaag cggaaaaccc cggcctgaac
    13261 ttcataggaa agtatcgtct tactggtgtt caccgaggag aggatctcac cggtactcac
    13321 attgacgaca cgcaggttca cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat
    13381 cccaaaatat cttgccccaa caccgcccga tttaacgttg ctttcataac cgataatcga
    13441 accttcaacc atgatatttg ccgccgttaa agattgcagc gggattcggt tattaatggc
    13501 aactgtgccg ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca ggttttgtaa
    13561 actctgacgc tccagcggta taaaccagcg agaatctttc aatgccgtga ccagcattgc
    13621 cgtggcgctt tgcggaacag cagtggagaa gttacttgcc gggtagggtt taaattgccc
    13681 ggtttcgtcc tgaatgttgt ataccgaaac aaagatctta cccgtcggcg ctggcagatg
    13741 ggtcaaatct ttgtagctct gagcttgagg cattaatgtc ggtctggcgg cttctttagg
    13801 cggggcggtt aagcatccgc tcagtaacat gacggcaacc aaaagaaata agcgctgcat
    13861 gattattttc cttatgaagc tggggcttaa aaatcggttg agttattttg taaacccgaa
    13921 acctggatgg tcgaggtttg tccggtttta cgatctgtca cgttcaactg caattgacca
    13981 tcgcggttag caatatcaac aatataatcg ttggtcacca tgcggcccgg tttaccggta
    14041 ttaatattcg acagtagccc acctaaaatt tgtgactgga tggcctgagt aaagttatct
    14101 aacgctgagg gtgtttcaat accaaagtca tcgttatagc tcggatcttt ataagagttt
    14161 tgggcctgag cgctatttaa taaaaaagcg ccattatttg ggttaccacc aaagtttgga
    14221 ttacggaact ggaaagtcat ggttccagcc caacttaatg gcgaaataag catgagtaga
    14281 actactgcat gtttgacacg cattgcagcc tccgaacaat tttttattta gaattcatca
    14341 tgcgccaaat cgcccgtact taataacgcc tgatttatct ggcgacgatt tagtgcttct
    14401 tcagtttgaa tcagtgcaaa gacgacagtt ttctcgaagt cttttttcaa cggaaataaa
    14461 aaagtctgga aaataacgtc ctgattgacc gttatagtga tccagcttcc ccatcgtgca
    14521 ctgggccttt cattaatcgt taagttaccc gtatagtcac tttcccattt atcactaaag
    14581 gctcggtaaa aatcatggcc aatagatgaa acagtatggt cagttagcaa tcccgggact
    14641 tctacctcaa cggcgtgaag attccctgcg gcgaacagaa attctgccgc cacaatccag
    14701 cgtaaataac gtttcatggc tttatcgcct gaggttatcg tttgcccagg aaaccgcttg
    14761 tgtccggttt tttacggcta tcttcttgaa aagattataa agatgcgttt taaccgtatt
    14821 ttcgctgatg aacaacgaac gagcgatctc gttattagac gcgccgatac gcagtttatt
    14881 caggatctct ttttcccgat gagtaaggag ggctgattcc gtgctgttat aacgatagtt
    14941 acctgaatgc gtaatcaagt agctggcaag cttttgcgta aagtagcatt cgccgcgcag
    15001 gacgccttgc aacccattga caacacgttc ttgatcctcc atggcataaa aaacgccgtt
    15061 gatatgaggc cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat ttagcaacaa
    15121 tattttgata ttgttgtttt tcctgctcaa agtatcctgc caataatgga taagcttttt
    15181 atccgcttcc atcatatcca gaagaataat agagcctgaa gatatatcgt c
//
LOCUS       DAZOYS010000092        15211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOYS010000092 DAZOYS010000000
VERSION     DAZOYS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.092"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(160..1209)
                     /gene="yahK"
                     /locus_tag="ACHUN9_002489"
     CDS             complement(160..1209)
                     /gene="yahK"
                     /locus_tag="ACHUN9_002489"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0695855.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1296..1370)
                     /locus_tag="ACHUN9_002490"
                     /pseudo
     CDS             complement(1296..1370)
                     /locus_tag="ACHUN9_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1586..2968)
                     /locus_tag="ACHUN9_002491"
     CDS             complement(1586..2968)
                     /locus_tag="ACHUN9_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0695856.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2978..3928)
                     /locus_tag="ACHUN9_002492"
     CDS             complement(2978..3928)
                     /locus_tag="ACHUN9_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0695857.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(4071..5489)
                     /locus_tag="ACHUN9_002493"
     CDS             complement(4071..5489)
                     /locus_tag="ACHUN9_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0695858.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5489..7036)
                     /gene="fdrA"
                     /locus_tag="ACHUN9_002494"
     CDS             complement(5489..7036)
                     /gene="fdrA"
                     /locus_tag="ACHUN9_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0695859.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7026..7889)
                     /locus_tag="ACHUN9_002495"
     CDS             complement(7026..7889)
                     /locus_tag="ACHUN9_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0695860.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7929..8534)
                     /locus_tag="ACHUN9_002496"
     CDS             complement(7929..8534)
                     /locus_tag="ACHUN9_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0695861.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDYTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8792..9289
                     /locus_tag="ACHUN9_002497"
     CDS             8792..9289
                     /locus_tag="ACHUN9_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0695862.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9381..10313
                     /locus_tag="ACHUN9_002498"
     CDS             9381..10313
                     /locus_tag="ACHUN9_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0695863.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10355..11443)
                     /locus_tag="ACHUN9_002499"
     CDS             complement(10355..11443)
                     /locus_tag="ACHUN9_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0695864.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(11585..>15211)
                     /gene="ehaA"
                     /locus_tag="ACHUN9_002500"
     CDS             complement(11585..>15211)
                     /gene="ehaA"
                     /locus_tag="ACHUN9_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR0695865.1"
                     /translation="TGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKG
                     TSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQ
                     NSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEK
                     KITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGIT
                     QNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVAS
                     GAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFA
                     YTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVD
                     FGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNS
                     AIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVN
                     NDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVS
                     LSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSAD
                     SLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLV
                     SHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVL
                     VVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIE
                     LIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPN
                     PEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYY
                     TDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWH
                     VGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSW
                     FDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGV
                     KADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSK
                     DFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGI
                     KWQF"
BASE COUNT         3448 a         4173 c         3652 g         3938 t
ORIGIN      
        1 aacgtgagaa gtattgtgta ctggagggag ttaatttaac aatttgaatt tacgttatag
       61 gttttactgc tactttgata ttatttcgct ctccttaact aaaaagttaa gtggagagcg
      121 aagttaatat gttaaaccac agcgtattta ttaatttttt caatctgtta gtgtgcgatt
      181 atcgataaca aaacgatatt tcacatcacc acgcagcatt cgctcatagg cttcattaat
      241 ttgatcggcc cgaatcatct ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag
      301 catctcctga gtttctggaa tgccgccaat catagaatcg gctatcgcac ggcgtttcat
      361 gatcaggttg aaaacttccg gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat
      421 ggtgccatca cgcttcagca aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt
      481 attcaaaatg aaatcgaaac tcttcagatg agccgccatc tcatcggcat tgcgtgagtt
      541 aacaacttca tcggccccca gggctttttc cgcttcgcgt tttgcctcag aagtggtaaa
      601 tgccaccaca tgtgccccca tcgcgtgggc cagcttaatc cccatatgtc ccagaccgcc
      661 gatgccgacc acgcccactt ttttacccgg cccggcctgc cagtgacgta gcggcgagta
      721 cgtggtgatc cctgcacaca acaaaggagc caccgccgcc agctgctctt gcgggtgacg
      781 aatacgcaga acatatcgct catgaacgac gatctgttgt gagtagccgc ccagagtatg
      841 gcccggttcg tccggcgtcg gcgagttata ggtgccgatc atgtgatcac agtagttttc
      901 cagcccgtcc tcacactctt cgcaatgttt acaactgtcg acaatgcagc cgacaccgac
      961 cagatcgccc ggcgcatgtt tttctacctg atcaccaacg gctaccacac gccccacaat
     1021 ttcatgaccc ggtacgcagg ggtaaaccgt ccccgcccac tcggaacgga cctggtggag
     1081 atcggaatgg cagacgccac agtaagcgat ttcgattttg acatcattcg gtcccggttc
     1141 acgccgtgtg atatccatcg gttcaagtgg ttgtttagcg gaatatgcac caacagcttt
     1201 gatcttcatt gtgtttactc ctgattagct atgtgtattt ggcgacgcct gatatgtcat
     1261 cgcggcaaaa cgcgtccatt gaatatagcc aatatttaaa ccacccgatc cagccccaac
     1321 atgccgcgaa cctgaatgaa acagagcagc aacgttcccc ataaggcgag cgcctataag
     1381 tgtgatattt gctatccgta aaagactaaa atcggcaaaa ctgatgatct tttcacgtag
     1441 acaaatagtt atctacacaa cgtcaataag ggcatattcc tcaaagataa tgtccatttc
     1501 tgccgacact atcatattga ggtcgcccca ctccctgggg cttaaatctc ttacaattca
     1561 ccgttgcagg cgtttcaaat gcgcatcaac ctgccacact cccccacacc agttgccctt
     1621 tatggaacgt tgcggttctc ggcgagatac gcgccaccgc ctcggcggaa caggaggcgt
     1681 ccaccagcac aaagctggca tcatcctgcg cttttggcca tacacgctcg cctttttcat
     1741 tcagcggcaa tacatcgcca gtggctagaa acagcgaacg ggagaggttc tgttcgttag
     1801 gacgaatata gagctgcgcg tagagattgg ctttttccag catgtcgccc agaccataag
     1861 gcgaccagtg gtcgataacg ctgtcagtgc cagtcatcac ttttacgcct ttgtcgtgca
     1921 actgtttgag cggcatatgc agcgtgccaa tcggcaccgt cgaggcgata gaaatttgct
     1981 gcgccgccat ccgattcgcc agcgcatcga cctgttgttc gttgagcgta gccagcgcaa
     2041 aggcgtggct gatggtcagc ttacctttca gttgcggcgt tttctctacc gtttcaacca
     2101 tataattgat ggctgccacg cccgacggag tggtttcgtg caggtgaata tcgacacctt
     2161 tgtcgtagtc cagcgcaatc tgaaacatgg tgtcgaggga tttttccatc gcgccatcga
     2221 cactggtcgg gtccagcccg ccgacgtaat gcgcacccgc ctgcatcgct tcgcgcatta
     2281 agggttccga tttcgacagc agcaaaccgt gctgcgggaa ggcgacgatt tcacactcga
     2341 agcccgcctg acgccgcgcc agcaccgcct gcaaattttg cagatttttt aggccggaaa
     2401 ccggttcgat attgcagtgg ctgcgggcaa tggtggtgcc tttcgactgc aatagatcga
     2461 tcagtttttc cgcccgctcc tgcgtatacg gttgtaactc cggcagcatt ttctgcttga
     2521 gtttgatcat gtcctggata gtggtgcctg ccggacgatt gagcgagcgc cacggcccgc
     2581 cgtagaaggt tttgtcgaga tgaatatgca tgtcgcgagt ggtgggtagc atcaacttac
     2641 cgccagcgtc atagtgcggc agcgtggcgt ccggatgctg cttgttctcg cgcagggcga
     2701 caattttgcc gtcctgaatc tccacggtct ggcgcgccgt gcgggtctgc accgccacac
     2761 cgttttcgta gtcaaaaccg gtttccagca gcacgttatc gagatagtaa tgcgggtcag
     2821 tgattttcat ggtgttgttc gcttcgcagt ttagggtgcc agctaccgcc gcatgggcga
     2881 gcggcacata ggtaccaaac agcgcggcgg cggtgaccat cttaccgctc tggctcagaa
     2941 attcacggcg gctattgctt tctttcatcc tacgtcctta cgttttaata atatgggtgc
     3001 ccgtttcacc gcgcagcgcc gcaggcaggc attccggcgt ggtgataatc acttctttgc
     3061 cgccctgctc caggaacgtc aggctggcga tgatttttgg caacatgctg ccgggagaaa
     3121 aatgcccttc ctgcatatag cgagtcatgg tggcaatatc cacccgatcc agcgcctgct
     3181 gctgcggttt accaaagtgg atacagactt tttcgacgtc ggtggtgatc accagaatgt
     3241 cggcgtgaat ttcacgggcc agcagcgcgg tagagagatc tttgtcaata accgcgtcca
     3301 cgctttggta atcccccgct tcagtacgca ctaccggaat tccaccgccg cccgcgccaa
     3361 ttacgacaaa accttgttgg atcagcgcct taatggcggg tgcttcgaca atacgtttcg
     3421 gttccggcga ggcgaccacg cgacgatagc cccgtccggc atcttcaaca aaacaccagt
     3481 cagggtttgc cttttgtagt ttgtcacgct ggctttcact gaagaatgca ccaatgggct
     3541 tggtgggatg ggcaaaacct ggatcgtttt tatccacttc cacctgagtt accacggtga
     3601 cggctttttt ctcgccgtga cgcgccagtc ggttattcag cgcctgttgg atcagatagc
     3661 cgatgccgcc ctgcgtatct gccacacagt tcgccagcgg cgttaagggc agcccttcgc
     3721 gctcgtgggc aatctccgca cggcgtaaat ccagcccgac ctgcggcccg ttaccgtggg
     3781 tcagcacaat gtcgtaatcg gaagccagca tttccagcac cgtatcggcc acggctttca
     3841 ccgcctccgc ctgatgctca atcgactggc tggcgttatc tttgataatg ctgttgccac
     3901 caatggcaac gaccacaagc tctttcatgt tgttatgtcc gctacagagg gtgatgaggt
     3961 tatgaccaac gccagactgc ctgatgcgct acgcttatca ggcctacaat tctcgggcca
     4021 tccgtaggcc ggataaggcg ttcacgccgc atccggcata aacatgtaag tcagacgccg
     4081 taacgttcgc accagccgag aatggctttc tcgaagcaag cgaatggtgg atgcacaatg
     4141 cccgcgccaa tcatgccgac gcccgcatct ttatgggcga tggcggtgtt gatcaccggc
     4201 agaatgccgc tgctacccac gcgggtgatg tcgattgccg acggcacgcc cataaaaccg
     4261 agcagcggaa tggtgacgtt ggggttttca ccgagagtga tttcacgcat ctgacgggag
     4321 aaatcgatag cctcttccac cgtgccgccc accagcgcga cgattgcagg cgcggtcgcc
     4381 atagcaaatc caccaatacc gtaggtttcg gtgatggcgc tgtcgccgat atccagcccc
     4441 gaatcttccg gcttataacc ggcaaacatc gggccgatca cctgctgcgc cgggccggta
     4501 aaccattgcc ccggcaggcc gctgacccgc aggccgaact cgacgccgtt acgcgccatg
     4561 gtggtgacca cggtgctgta ttcgatgccg tgcgccgcat ccatcgccgc tttacacatc
     4621 gccatccacg tcgggccgga gaagtagtcg ctgctggcga caaactcgaa cacttcgcgc
     4681 tgttgctcga cggaatagcc tgcctgaatg atccccggcg tcagtgcctg aatcagcagc
     4741 gtcgtcccgg cgttattgcg gttatggcac tcatcgccca tatgcagcgc ctgcgccagc
     4801 attaagcgca gatcgatctc accgataatt ttcatcgcgt cgcgcagcat tggcccctgc
     4861 acatcgcgca tccagttgag gcggtcgatc acgctctgat cgttggcacc catacgcaaa
     4921 atcttcgcca tctgctcgct catattggtg taagcgatgt tgccgtaggt tttgtttttc
     4981 acgatgtgca taaacatcga ggccgaggta acgcccgcca tcgatcccac acagtcgtgt
     5041 tcgtggcacg gcgagaaggt gatctccccg gaggccgcca gttcagccgc ctcgtcgaga
     5101 tctttcgcca gtccttcgaa caccagcgct ccggtgaccg cgcctttcat cgcgccgcac
     5161 attttttccc aggtgatcgg cggcccggcg tgaagaatgg ttttcggcgt catgcccggc
     5221 accacgttaa tcgcctgatc aaaaccaatc agcaccggat gcgactggat aatgcgctcc
     5281 agcgcctgct ggttagcggc ggcgattttg tctgccagcg gcgaatcggc aatgttatcc
     5341 agcgcctgca ccacctgcat attgccctgc cccggcggcg tccagtcgag ttgagtcact
     5401 tctacatgct gctttttcag gtcatcgcta aacatggcga tgccgacgtt aataacgttc
     5461 aatggttggc taaacagtga ctggctcatc aggcttcctc ccctttgcag ataaattcac
     5521 gcgccagcaa tccggtattg gtgctgctgc tcgccagaat cactccggca tcaagcagca
     5581 tctggctttg ttgttctaac gatggcgtat caagatcggt acccagcaca taggcgagaa
     5641 tgatcaactc gcgtccttcg gcagcggcga tcgctttcgc ttctttgatc gcctcgatgg
     5701 tggagccgac cggatcttca tgcgatccaa atccgagcac aaaatccatc acgatcaccg
     5761 ccacttctgg atcgcgcgcc tcttcgatca agcgactgat gcggttggtg gggtcaatca
     5821 ttggatgcgg cttgccgttc gtgaagtcgt catcgccaaa gtcgaggaag gtgtgtttga
     5881 tgctgcggtt gatatcttgc aggcggaatt ccggatcggg ctgaatgttg ctgtagacat
     5941 cgccatgttt ttccatcacc gcgaacatgg tttcgtcgca cagcgtgccg ccgcagaaca
     6001 ggccacgaat gtatttctgc tgcggttgca gacgcgcacg cacatcagca atcaacggct
     6061 ggttaagcgt atgcaggtcg agattttcct gtttcacgcc ggagagcatc accgctttta
     6121 gcgccgcctc tttgctgccg cgggcaaact gtagcccctg ctcatccacc ggcgtttcaa
     6181 cgcgaccgag gaagcagacc accaccggct tgcggcaggc acgtgcgcgt tccagcactt
     6241 tgcgggccac cgcaggcgcg ggaggttttg aaatcagtgc gataatttca gtttgcggat
     6301 cgttttccag catcccgatg gcgtcgagca tcatcaggcc gccgattttc tcgctcaggt
     6361 cgcgcccgcc ggtgccaatc agttgtgaaa caccgccacc aaattcatga atgcggacgc
     6421 tcagctcctg gctgccggtg ccggatgcgc caacaatacc gatattgccg cgacgcacgg
     6481 cgttaccaaa acagagcgcc gcgccgttaa taatcgccgt gccgcagtcc ggccccatca
     6541 tcagcagccc tttttcgtgg gccaactgct tgagcgccag ttcatcttca actgagacgt
     6601 tatcggaaaa cagcatcacg ttgagatcgt tttgcagcgc ctgacgcgct tcgcgagcgg
     6661 caaacagacc gttgaccgaa atcaccgcca ggttactttc cgggatatgc tttttggcgc
     6721 tggcaatagt ggcgtaacgc gcctcgtgcg agccgctttg cgctttggtg ttgaacagtt
     6781 cttcaatctc caccagcaac tgctcgttgt ccgcacccga tttaccattg atgacaatca
     6841 tcaggtcgcc gtttttcgcc tgttccagcg tcggcgtcag cagtcctaaa tttttcagca
     6901 cacctttgtt catttcggtc gccatcgcca caaatgcctg ctcgacgccg tcgagtttat
     6961 ttgcacgcgt ggagatagac atcagcgaga cagaatcaaa ataggtattc ggtttaatga
     7021 ctattttaac tgacataatt ccctccgtag gtttggctca gcgcacaacc atcggccatg
     7081 ccatacagca tgtcgcagcc ggaactggag ccaatatgtt taattttttc tattgcctga
     7141 gttgaattcc cagggacgcc ataaataatg ttgtgaataa agcgatgaat attttcccgg
     7201 cagcgttgtt gtaatgcggc ttccagcgtt atggcactta ataacgtggt gttatttctg
     7261 ccgcgtagca gaccgcgata aaattcctct ttgtattttt ctgccggatg cccgggaata
     7321 aataaaataa gcgccagacc tgttaaataa tcatcggctg atggcgtaag gccaatccct
     7381 aacccaatca tactggcgac cgctgcggcg atattttgct tatcgtttac tgtctgaata
     7441 actgcccgtc gtttaatatg taattcttta tttaacgcct gataaaacgg attatcgccg
     7501 cgataattaa acagggtttc attttccccc aggtgctgat gaataatgtc gtgccactgt
     7561 agccaggcga taaggtgaaa attcacagcg gtcatttgca aggttgggga cagccaacgc
     7621 cgacaacgac tcatctctat atgaagatgt tgaccaaccg taataccttg atcgtcaaac
     7681 tgaaccttat caccatgccg gaacagatca tcaaagtgag tgagtgccaa ccgacaactg
     7741 tttggcgcat tgtcgtactc ttcacaaagc agcgtcagca actgctggcg agccgggagt
     7801 gcgatattta ccgctctggc aaagacctgt tcaacctgtc cttgcccccg ctgcgccaga
     7861 aaatccgcat ccgcagttag cgcccacaca caccgttttt ctggtgtgtt gatagccagc
     7921 ctcccggttt atgcacctgc ggcaatcagt aactgcgcaa tctcttcaaa gccccgttcc
     7981 cgcgccagtt ccagtggcgt tttgccatat ttatcggtca gatgcgggct ggcaccgtgt
     8041 tccagcaata actgcacaat cgcctgctgt ttaataccac catcattaag cacaatcgct
     8101 tccagtagcg gcgtccagcc tacatggttg gtctggttaa cgttaatctc cgtatgcgcc
     8161 aaaagctctt ttacaatact taaatggcct ttttcacagg caggcgtcag gccgacaccg
     8221 ccaaaacggg ttacgcaatt aagatcgggt ttagccggta aaataattcg tagtagcgtt
     8281 aaatcatcgt tcagacagct aattaaaaaa ggatttaaac aggtataatc ttgcttatta
     8341 atatccgctc cggcatcaat taatgcctga acgcaagcat attgctgata taaacttgcc
     8401 agcgtaattg ccgttttccc ctgacgatcg caggtattaa tatcgacacc cagcgcaagg
     8461 caggttttta ctttatcgat atcaccctgt tgtgcagcca ataaatagtc ggcaggtaga
     8521 ttttttatac tcattcacat tcccctggtt tttgtggctg ttttcgatga attgagaata
     8581 gcaacgataa tctgataaaa gaacccgctt aaatttaatt cgccgtatct cgcctttttg
     8641 ttgaataata ttcaacaatc ttttcgcagt gtgcggtagt gcaatatttg tttctttgac
     8701 ccgataccct ttcactcaat caggttttat ttctctatag tctgcacatg taagagtagc
     8761 tttcgtccat gtctaaccag aggggaacaa gatgaacgga ctcacggcaa cgggtgtcac
     8821 cgtcggtatt tgtgcagggc tttggcagtt agtgtcttca catgtcgggc tctctcaagg
     8881 atgggaatta ttaggaacca ttggcttcgt cgccttttgc agtttttatg ccgcaggcgg
     8941 cggtaaatcg gggtttatca gaagtctggc ggttaattat tccgggatgg tgtgggcgtt
     9001 tttcgccgcg ctgactgctg gttggctggc ctctgtaagt ggactgtccg cgttctgggc
     9061 gagcgtaatt actaccgtac ccttctcggc ggtagtggtc tggcaagggc gtttctggtt
     9121 actgtccttt attcccggtg gctttctcgg catgacgctg ttttttgcca gcgggatgaa
     9181 ctggacggtg acgttactgg gttttctggc gggtaactgc gtggggataa tttcagaata
     9241 tggtggacaa aaattaagcg aagcgacgac taaacgcgac gggtattaaa agttattcaa
     9301 aaatgattat ctatctttaa ccgatacctc tacagtcata gctggagaaa ttcatagtag
     9361 ataatggcga gaatggttta atgaactcaa tttttaccga agagaatctg ttggcattta
     9421 ctaccgctgc gcgttttggc agcttcagta aagccgcaga agagttgggt ttaaccactt
     9481 ccgccattag ctacaccatc aagcgtatgg agacggggct ggatgtggtg ctgttcactc
     9541 gcagtacccg cagcattgag ttaacggagt ccgggcgtta ttttttccgc aaagctaccg
     9601 acctgctgaa tgatttttat gccatcaaac gcagtatcga tactatttcg cagggcatag
     9661 aagcgcgtgt gcgcatttgt attaatcagc tgctttatac gcccaaacac accgcacgct
     9721 tgttacaggt gctgaaaaag cagtttccta cctgccagat taccgtcacc accgaagtgt
     9781 ataacggcgt ctgggatgcg attattaata atcaggccaa tatcgccatc ggcgcacccg
     9841 atacactact ggacggcggc ggtattgatt acactgaaat tggcgcgatt cgctgggctt
     9901 ttgctatcgc cccggatcac ccgttagcct ttgtgccgga acccatcgcc gaaagccaac
     9961 tgcgtctcta ccctaatatt atggtggagg acaccgcgca tacgattaat aaaaaagtgg
    10021 gctggttgct gcacgggcag gagtcaattc tggtgccaga tttcaacacc aaatgtcagt
    10081 gccagatttt aggtgaagga attggttttt tgccggatta catggtccgt gaggcgatga
    10141 cgcaatccct gctggttacc cgacaaatcc ataatccgcg ccaggattcg cgcatgttac
    10201 tggcgactca gcattctgct acgggtcagg tgacgcagtg gattaaaaaa caatttgcgc
    10261 caaatggcat attaacgggg atctatcagg atttactgca tcgggagaat taatcgctgc
    10321 caacctcccg atgcgctgtg aatagcggtt tacatcaacc acctgctttc attacccatt
    10381 cagagataaa tttattaccc ggtactggcg gagagtacaa atagccctgc aaaaagtgaa
    10441 cgcctttacc gatcattaaa tccgcctgct cctgggtttc taccccttcc gccacgatac
    10501 tcagaccagg cttacgcgct agttcgacaa tattgtccac aatatgaccg gagatttcgt
    10561 cgacactcgc catttgcaca aatgacttat cgatcttaat aaaatcgacc gggaacgcct
    10621 gcaagtaacg ataggtcgca taacccgtac caaagtcatc cagcgcaaag gtaatgttgt
    10681 gctgatgaag gctgtcaaat atcgctctgg cttctggcgt taccggaata gggttacgtt
    10741 ccgttagctc gagaaccagc ttgattttat cgttacctaa tttcttaacc aggttcagac
    10801 actctttttc aaatcccgct gccaaaaaac aacccgccga gacgttgatg ccaatatgga
    10861 aattgtccgg cagcaaatgt tttaccggca tcagaatatc cgcagtctgt ttcatcagtt
    10921 ggcgggtcat tatgacaata agaccggatg actccgccag aggaataaac tgatccggtg
    10981 ggataattcc cgtttgtgga tgttcccagc ggacaagtac ctcacagccc gtcagtaccc
    11041 cagtctgcgc gcagaaaacc ggttggatcc acggtttgaa ttcgtggttt tccaacgcca
    11101 gactgatggc ttcaggcgtg acgatatggt gacaacggtt atcagggata taaaaattat
    11161 ttttatttcc cgtgccactg atgacttgcg gatgatattg aaaaaagata tcccgccaga
    11221 aggtaatatc actctggatg cctagttttt catacagctg cttcttttga tgtgaaattg
    11281 tctttgcact gcgatttctg tactgtgaga tttctgtaac cgacattccc tccattagca
    11341 gcttgagtaa aagccgttct ttttcggaca cgataccagg caacgtcaaa ttcaccgttg
    11401 ccccgaactc ttccaaaaaa acactcaaat catgcgaagc cattgaacac accttgatta
    11461 aatcttgtgc gccagctaaa tctgcgagtc ttcactctga ctgataaagc tggctgcaag
    11521 aaaatgacac agagagccga attatctccc gttcggctct ctctcctttt actcattatt
    11581 cagatcagaa ttgccactta atgccaacca ttgcagaggt gtcattatat cccctatcgg
    11641 caacctgaac gcccacattc ccccagacat tcaggttggc atttagctgt ccttccaccc
    11701 cggtttttat ctcagcaata tttcgggctc catcctgagt gacggacacg ccatccatac
    11761 tggtgctgaa atccttactg ttatgcagcc agttcacttc tacaaacggc tggaactcgc
    11821 gggatttacc gtcatccatt ttatggtggc tcttcagcca ggtttttacg ccaagtcggg
    11881 tttgaacatt gccatcaccg ttgctatgaa cgagggttcc gttgctttcg cggtgcttat
    11941 cggctttgac tcccatccag gtaacctgtg cttgcggctg aacataccat tcattacgcg
    12001 ttccctggct gccattaaat tcagctaatt tgtgtttgta tccagcttcc agtgaagcgg
    12061 taaatccttt tgatttatag gattcacttt gtaagtcatc ccctttcact gtgttatcaa
    12121 accagctgta ctgcgcccaa ctgtcgagat acgcgccatt acgcgactcg tcatcggcat
    12181 accaggtggc atagaggccg gtgctatatc cgttcacact cgcttttgca cgataaccgg
    12241 ttcgcgagga aatggttttg ctgtcgctgt tgccatatcc cgccatgacc ccaacatgcc
    12301 agctgtcgct gccgttttgg ctccactgcg cgacatcgcc tcccagttgc agaacatagc
    12361 gattgctttg ggttttcagc tggccgctgc catcacgcca tttattatga ccaccttcat
    12421 ggcgcatcca catagtggtt tgtttctgct cacccgtcac catgtcggta tagtacgtat
    12481 tacccagacg ctcatgcaga cgcgtggtga acattgtatt cgctgctgca aggttcgcaa
    12541 tgtagctacc cgcctccggt cgcaggtcat tatccacatt cagatccgga cccggcgtag
    12601 gtgtcgggtt agggttcggc tctggattcg ggtttggctc cggattcggc atcgggtctg
    12661 gctccggctg cagttcagga gaatcactgc cgctggtcag ataccagtta ccactatttg
    12721 ccccttgtcc acgcgcgaga gtgtagtcat acgcccccgc aacgatacga ccagcctgaa
    12781 caaattcgcc ctcagactta ccgtctacat ggataagttc gataccgtta agggttttcg
    12841 cacctgtacc gccagcgtta ttcaccgtaa cggcagtcgt gccgctagta tcgccctcga
    12901 caaccaattt atcggttact gagtcatcgc cattcagtac cgtattcatt actagttgac
    12961 cgttattacc gtggtagttc cccttcacca ctaacacatt gccagcttgc tgtcctgaca
    13021 ttccggtcat cagcgtaccg ttgttggtca gattgccgcc cagggtgtaa ttcacagacg
    13081 cagccgctct tttttcagcg ctattattca acgccagggt accgttgttc tcaacgtttg
    13141 cgctacccaa tccctgcgca tgacttacca ggacattagc cgaattagca atgttcacat
    13201 tcgctgtcag gttgctgtta tcgcccgcga tgtccagcgt gccgccctga atattcagct
    13261 ctccgctccc cgttaacgaa ccggttgaag tgcccccgtt ggtaaccgtc agactgccac
    13321 catttaagct cagcagtgaa tcggcgctgc cattgagcgt gcccacggtc tggctgtgac
    13381 cattcatatc cagttcagtc tctgccgcca gacgcagttc atgggtatta cccaacacgt
    13441 tatcgttatt caacaaaagc gtcccactgc gcagatcggt aacaccggta tagtcgttgt
    13501 ctttgttaga aagcgatacg gtctgaccct tctggctgct gaatgccagg tcaccactgc
    13561 cggtaatctt tgcgctgaga tcggcggcat tctcgctttt accgttagag ctcagcacca
    13621 gagcatcgct gtcggtagcg tgtaaatcaa gctgggtcag gccgtaaccg atatacagac
    13681 cgtcattgtt taccccactc gtcagacgat agtcgtaagt gccgacggcc gctttttgcc
    13741 cattctgctg aatatcacgt tgggcactgc tggagaggat ttgcccattt ttatcctgca
    13801 attgcagatt gcccgcatcg cccagaactt ccgcaccttg cgcatcaacc agcttaatgg
    13861 cgctgttacc atcatcgact tcagtgagcg ataacgcaga gtcaatatca cgagatactg
    13921 agcggacaac gtcactgtca gaaacctgaa tcacaccctc gccgcgcaaa tccagcgttt
    13981 tactaacgtt gaccgtccct tctgtcatct gtgcacctgc tgtcagggca ccaaaatcaa
    14041 cggttccgcc ggagaatccc aaagtgctaa tggagtgagc gcccttaccc actgttacca
    14101 ggcttccctg acccgcagtg atcccgcctc tctgcagaaa ctcagcattc ttttccaggt
    14161 taaatgtcgt gttctggaac gccagtgaac cctggaacca gtctgtataa gcaaactcat
    14221 cgaaggagaa ttcgtgattc cccatgttta tttcaacaat acctctacct tgaagatcgt
    14281 tatcaaatac ccaatcttta ttggcattga tttgcagttt gccatcaaca tccaggtccg
    14341 cacggccgat gttcttgtct tcagaaataa ccgcgacagc accagaagcg acatcaatat
    14401 atccgctgaa tgcatagtta ttgccggtca gcgccactgt cccactgtcg atacttattg
    14461 taccgttgcc ggaaatctta ttggcaaagg tcatgctatc gtcagccttc aggtgtaatt
    14521 tcccgcggtt gttgatttct gcggtaccta tattttgggt gatccctaaa gcgccatttt
    14581 gctcaatttt gactttaccg ctaaaggcgg agttatcccc ggttaagtaa gtcgtaccat
    14641 tgagcgcatt gatatagccc aggccctcgc tgtcactgac aatatccgcg gaaatagtcg
    14701 cgtttttatc cgtgtggttt aaggtgattt tcttctcgga tgtctttgcc cacacaaact
    14761 caattttttc ggcatcaata atccctgcag ccttcgctgc gcttccttcc tgtgcaccta
    14821 acgctaactc agagttggtg cttaaactaa tagtcggcgc agagatttta ccaccttccg
    14881 aaacagcaag tgttgtatcg gagttttgat cataataacc caggctaatt ttctcactgg
    14941 cattaacttc accgccgtta ttaactgaca ggtactgatt accaccgtaa ctgccgtaaa
    15001 cgcccataaa gaaggtttta gaatttacct ggctgccttc accactgaca tttccctgca
    15061 aataggtcag acggctggtg cccttaacat ggattttgcc gccgttggag acattaaaat
    15121 agccgcgatc gtgtctcatg cctgagtttg actcatacaa atcatcagca tataactcgc
    15181 tgccagcatt ggagacattc aggaaccccg t
//
LOCUS       DAZOYS010000093        15171 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOYS010000093 DAZOYS010000000
VERSION     DAZOYS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.093"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUN9_002501"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUN9_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0695866.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUN9_002502"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUN9_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0695867.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUN9_002503"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUN9_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0695868.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUN9_002504"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUN9_002504"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0695869.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUN9_002505"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUN9_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0695870.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUN9_002506"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUN9_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0695871.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUN9_002507"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUN9_002507"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0695872.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUN9_002508"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUN9_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0695873.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUN9_002509"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUN9_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0695874.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUN9_002510"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUN9_002510"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0695875.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUN9_002511"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUN9_002511"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0695876.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15171)
                     /locus_tag="ACHUN9_002512"
     CDS             complement(14275..>15171)
                     /locus_tag="ACHUN9_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR0695877.1"
                     /translation="SFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQ
                     VYMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHE
                     HLDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKG
                     NNHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPL
                     IQRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRIS
                     SLREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3741 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg a
//
LOCUS       DAZOYS010000094        15170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOYS010000094 DAZOYS010000000
VERSION     DAZOYS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.094"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..867
                     /locus_tag="ACHUN9_002513"
     CDS             <1..867
                     /locus_tag="ACHUN9_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0695878.1"
                     /translation="AISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRD
                     LLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLL
                     DFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKTR
                     EATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
     gene            complement(1070..1423)
                     /locus_tag="ACHUN9_002514"
     CDS             complement(1070..1423)
                     /locus_tag="ACHUN9_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0695879.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1561..3207)
                     /gene="groL"
                     /locus_tag="ACHUN9_002515"
     CDS             complement(1561..3207)
                     /gene="groL"
                     /locus_tag="ACHUN9_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0695880.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3251..3544)
                     /locus_tag="ACHUN9_002516"
     CDS             complement(3251..3544)
                     /locus_tag="ACHUN9_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0695881.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3820..5076
                     /gene="yjeH"
                     /locus_tag="ACHUN9_002517"
     CDS             3820..5076
                     /gene="yjeH"
                     /locus_tag="ACHUN9_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0695882.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5098..5568)
                     /locus_tag="ACHUN9_002518"
     CDS             complement(5098..5568)
                     /locus_tag="ACHUN9_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0695883.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5905..7341
                     /gene="aspA"
                     /locus_tag="ACHUN9_002519"
     CDS             5905..7341
                     /gene="aspA"
                     /locus_tag="ACHUN9_002519"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0695884.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7459..8760
                     /gene="dcuA"
                     /locus_tag="ACHUN9_002520"
     CDS             7459..8760
                     /gene="dcuA"
                     /locus_tag="ACHUN9_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0695885.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8876..9214
                     /gene="cutA"
                     /locus_tag="ACHUN9_002521"
     CDS             8876..9214
                     /gene="cutA"
                     /locus_tag="ACHUN9_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0695886.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9190..10887
                     /gene="dsbD"
                     /locus_tag="ACHUN9_002522"
     CDS             9190..10887
                     /gene="dsbD"
                     /locus_tag="ACHUN9_002522"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0695887.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10924..11499
                     /locus_tag="ACHUN9_002523"
     CDS             10924..11499
                     /locus_tag="ACHUN9_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0695888.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11606..11681
                     /locus_tag="ACHUN9_002524"
     tRNA            11606..11681
                     /locus_tag="ACHUN9_002524"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11639..11641,aa:Phe,seq:gaa)
     gene            11924..12038
                     /locus_tag="ACHUN9_002525"
                     /pseudo
     CDS             11924..12038
                     /locus_tag="ACHUN9_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12298..12597
                     /locus_tag="ACHUN9_002526"
     CDS             12298..12597
                     /locus_tag="ACHUN9_002526"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0695889.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12588..12713)
                     /gene="laoB"
                     /locus_tag="ACHUN9_002527"
     CDS             complement(12588..12713)
                     /gene="laoB"
                     /locus_tag="ACHUN9_002527"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0695890.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12780..13835
                     /gene="cadC"
                     /locus_tag="ACHUN9_002528"
     CDS             12780..13835
                     /gene="cadC"
                     /locus_tag="ACHUN9_002528"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0695891.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14200..>15170
                     /gene="cadB"
                     /locus_tag="ACHUN9_002529"
     CDS             14200..>15170
                     /gene="cadB"
                     /locus_tag="ACHUN9_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0695892.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3734 a         3779 c         3637 g         4020 t
ORIGIN      
        1 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
       61 ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat gtctgtcatg
      121 gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca taaactgaat
      181 gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa
      241 aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac
      301 acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac
      361 ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc
      421 attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta
      481 gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga atatgacacc
      541 tattcccaac cggaatggaa acataacctg ttcgaccatt atctggcggt gctttaccgc
      601 tttaaagatg aaagtggcaa agaacaattt agcggcacag tggtgaaaac gcgtgaagca
      661 acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag cccacgactg
      721 aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc gaataacgcc
      781 caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt tcaatccacc
      841 agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc tggagccatc
      901 cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcgacgt aaacgcctta
      961 tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag gcctgataag
     1021 cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat cagttcgggc
     1081 acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac aacattccct
     1141 gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc tggttgcgca
     1201 gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg gaaagccagt
     1261 tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc tgctcgtcca
     1321 caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa cccgccatca
     1381 gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc cccataatca
     1441 atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa gatgaccaaa
     1501 agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgagg tgcagggcaa
     1561 ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc ctaagtcagc
     1621 tgcatcattt ttcggcaggt cggtaaccat gcactcggtg gtgatcatca ggccagccac
     1681 agaagccgcg tactgcagag cagaacgggt tactttggtt gggtccagga tacccatgtc
     1741 gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt cgccgccttt
     1801 aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct gacgcagcgg
     1861 agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt tctgaccacg
     1921 caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag caaccacgcc
     1981 ttcttctacc gcagcacggg tcgcgtgcag ggcgtcttca acgcgtgctt ttttctcttt
     2041 catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg ccagtttcgc
     2101 tacgcgttcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg
     2161 gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga tgatagtggt
     2221 ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg ttgctttttc
     2281 cagttccata ccgatctctt cagagatcac ggtaccgcca gtcagggttg cgatatcctg
     2341 cagcatagct ttacgacgat cgccgaagcc cggagcttta actgcagcaa ctttcacgat
     2401 gccacgcatg gtgttaacaa ccagagttgc cagcgcttcg ccttctacat cttcagcgat
     2461 gatcagcagc ggtttgcctg ctttggcaac ggcttccaga accggcagca tttcgcggat
     2521 gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta ctgcgccagt
     2581 ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca taccttcaac
     2641 cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc cttctttacc
     2701 gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt tagcggagat
     2761 ggtaccaacc tgagcaatcg ctttagagtc agagcacggt acggacagcg ctttcagttc
     2821 ttcaactgca gcggtaacgg ctttgtcgat accacgtttc aggtccatcg ggttcatgcc
     2881 cgcagcaaca gctttcagac cctcagtgat gatagcctga gccagtacag ttgcagtggt
     2941 ggtaccgtcg cctgcagcgt cgttcgcttt agaggcaact tctttcacca tctgcgcacc
     3001 catgttttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac catctttggt
     3061 gatggtcggt gcaccgaaag atttatccag aactacgtta cggcctttcg gaccgagggt
     3121 aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac gagcgtcgtt
     3181 accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta tgttcagtgt
     3241 cgtgcgagga ttacgcttca acaattgcca gaatgtcgct ttcggacatg atcaacactt
     3301 cttcattgtc gatcttctca gatttcacac cgtagccatc gttgaaaata acgatgtcgc
     3361 caactttcac atccagcggc ttcacttcgc cattttcaag gatacggcca ttgccgacag
     3421 ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg atgccgccag
     3481 cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat ggacgaatat
     3541 tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc ggagcttacg
     3601 tggtttcccg gctggtgacc agagaaatgg ggatgaggat tcgccccttc aagggggaaa
     3661 aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac ctcacatttc
     3721 atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc gaaaatctac
     3781 gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact caaacaagaa
     3841 ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac cggcgtgttt
     3901 gccgttcctg cgttagctgc acaggtagca ggcaataaca gcctgtgggc gtggcccgtt
     3961 ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc
     4021 agcgcaggcg gtgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc
     4081 accggctggt tgtttttatc ggtcattccc gtgggtttgc ctgccgcgct acaaattgcc
     4141 gccgggttcg gccaggcaat gtttggctgg catagctggc aactgttgtt ggcagaactc
     4201 ggtacgctgg cgctggtgtg gtatatcggt actcgcggtg ccagttccag tgctaatcta
     4261 caaaccgtta ttgccggact tatcgtcgcg ctgattgtcg ctatctggtg ggcgggcgat
     4321 atcaaacctg cgaatatccc cttcccggca cctggtaata tcgaacttac cgggttattt
     4381 gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc
     4441 tcggaattta aaaatccaga gcgtgatttt cctcgcgcat tgatgattgg tctgctgctg
     4501 gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa
     4561 caaatggccg cggcagcatc gcttccaaaa attgtagtgc aactgttcgg tgtaggagcg
     4621 ttatggattg cctgcgtaat tggctatctg acctgctttg ccagtctcaa catttatata
     4681 cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca
     4741 cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgctcgg ctgctgcgtg
     4801 gtgagcactt tggtgattca tgctttagag atcaatctgg acgctcttat tatttatgcc
     4861 aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa
     4921 ggacgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg
     4981 gtcggctgga aaagtctcta tgcgctgatt atgctggcgg ggttatggct gtttctgcca
     5041 aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatccta
     5101 gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctggt actcaccatc
     5161 aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg
     5221 caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata aaagcagaag
     5281 accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa taatcagcga
     5341 aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt gctgcatcag
     5401 cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg acgtgaatat
     5461 aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa agattgaaat
     5521 ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg
     5581 taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt cagcttatat
     5641 tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt aatcccaaag
     5701 cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta aagtgatcca
     5761 gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga tgcaggggat
     5821 aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt
     5881 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg
     5941 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa
     6001 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta
     6061 atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta
     6121 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag
     6181 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa
     6241 gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata tcagtacctg
     6301 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc
     6361 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa
     6421 ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg tacccagctg
     6481 caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat cctgctgaaa
     6541 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggcgca
     6601 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa
     6661 ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct
     6721 gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc
     6781 aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct gaacgagatc
     6841 aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt
     6901 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg
     6961 gcagcagaag caggtcagct gcagttgaac gttatggagc cggtcattgg ccaggctatg
     7021 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac
     7081 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt
     7141 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc
     7201 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa
     7261 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat
     7321 actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaagaaggc acgtcagatg
     7381 acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc aacttgttaa
     7441 aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct ggcgatcttc
     7501 ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg ggtgctggtt
     7561 cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc cattatcatg
     7621 gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta tctggttcat
     7681 cagacagaaa agctgctgcg ccgtaacccg aaatacatca caatcctcgc accgatcgtg
     7741 acctatttcc tgacaatctt tgctggtact ggcaacatct ctctggcgac actgccagtt
     7801 atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctgtc tactgcagtg
     7861 gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt ttacatgtct
     7921 tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt catcccgtcc
     7981 accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa ctccaaactc
     8041 tctgacgatc cgatttatcg caagcgtctg gaagagggcc tggttgaact gcgcggtgaa
     8101 aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct gctgggcgta
     8161 gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt tgaaaaaccg
     8221 ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac tctgaccacc
     8281 gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa agcaggtatg
     8341 agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt ttccaacaac
     8401 atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg gctgctggcc
     8461 gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc aaaagcactg
     8521 atgccgatgg ctctggcact gaacgtttct ccgctgaccg ctgttgcttc tttcgctgcg
     8581 gtgtctggtc tgttcattct gccgacctat ccgacgctgg ttgctgcggt acagatggat
     8641 gacacgggta caacccgtat cggtaaattc gtcttcaacc atccgttctt catcccgggt
     8701 actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt catgctgtaa
     8761 tgaccaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca tccttccccc
     8821 gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg tgtttatgct
     8881 tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac cggatgaagc
     8941 gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct gcgcgacctt
     9001 gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag aatacgaagt
     9061 gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat gcctgaagtc
     9121 tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg gagacacaga
     9181 ttacctctca tggctcaacg catctttacg ctgatcctgc tactttgcag cacttccgtt
     9241 tttgccggat tattcgacgc gccgggacgt tcacattttg tccccgcgga tcaagccttt
     9301 gcttttgatt ttcagcaaaa ccaacatgac ctgaatctga cctggcagat caaagacggt
     9361 tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat tgccgacgtg
     9421 cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga gatttaccgc
     9481 gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac gttaactgtc
     9541 acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa aaccgttccg
     9601 ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgttcc gcagcaagag
     9661 cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg tattggtatc
     9721 gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt gctgggtggt
     9781 aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt gcaggggatg
     9841 gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca gttccaggcg
     9901 gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt gctggcgatg
     9961 tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacaca tctcacattg
    10021 atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg ggcgattgcc
    10081 ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct gtatatcgcc
    10141 caaagcggga acatgtggct gggcggcggc atgctttatc tctatgcgtt gggcatgggc
    10201 ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag cggcccgtgg
    10261 atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccgat cttcctgctg
    10321 gagcgagtga ttggtgatat atggggatta cgcttgtggt cggcgctggg tgtcgcattc
    10381 tttggctggg cctttatcac cagcctacag gccaaacgcg gctggatgcg cgtggtgcaa
    10441 ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg ggcatttggt
    10501 gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa aacggtagat
    10561 gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga tctttatgcc
    10621 gactggtgcg tcgcctgtaa aaagtttgag aaatacacct tcagcgaccc gcaggtgcaa
    10681 aaagcgttag cagacacggt cttacttcag gccaacgtca ctgctaacga cgcacaagat
    10741 gtggcgctgt taaagcatct taatgtcctt ggactaccga caattctctt ttttgacgga
    10801 caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga aaccttcagc
    10861 gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac ggaggaaaat
    10921 agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt acaagggatt
    10981 gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga cgagctacgc
    11041 cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct tagccaacag
    11101 atagatgtct ggcgtcgtca gctgatgctt gacgaaacgc aaaccgccga acaaaagctg
    11161 ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc gggctgtcta
    11221 tttatcgctg cctgtacgtt ttatcccgac cctggccacc ctattcatca actggccgat
    11281 cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct ggaagttgac
    11341 gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt aagccgaatg
    11401 ctggtgaatc gtagccaggc ggatgtcgac accgcacatc ggctggcgga agatatcctg
    11461 cgcttcgccc gctgccgcca gggtggtgca ctgacctgac agaaacacag aaaagaagcg
    11521 atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga cgagatgtgc
    11581 agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc aggggattga
    11641 aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat aaacggacct
    11701 ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc ctccaaatca
    11761 attctaccga actcaaccag attctcccca catcaccagc attttgcggg tatatcccaa
    11821 ttcgggaaaa tttgttcctg agcgatagca ctgactgacg tgaaatgtcg tgcggccccg
    11881 tgatgttgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgcatccata aagcacaaca
    11941 ccggtctgaa cggcgactga tgagattttc tgaatgagaa caaagagaaa tgtatcagtc
    12001 cgcttgctca tgcaaagact aacaatccat taaaatagta agcgctccgg acaattttcc
    12061 atggattatt ttctacacat ttttctttgg caaagatgat gaattttgat ggtaaggaat
    12121 attacttctg gttctcagta aaatcctttc gtaatactat gtaatcaaga agtttatggc
    12181 tggtaaaaat aacgtcttgc attcaccaat aacatgtaaa taaacccatc tattgatggt
    12241 aaaaataggt catggcaatt atcattgcac cattcccttt tcgaatgagt ttctattatg
    12301 caacaacctg tagttcgcgt tggcgaatgg cttgttactc cgtccataaa ccaaattagc
    12361 cgcaatgggc gtcaacttac ccttgagccg agattaattg atcttctggt tttctttgct
    12421 caacacagtg gcgaagtact tagcagggat gaacttatcg ataatgtctg gaagagaagt
    12481 attgtcacca atcacgttgt gacgcagagt atctcagaac tacgtaagtc attaaaagat
    12541 aatgatgaag atagtcctgt ctatatcgct actgtaccaa agcgcggcta taaatgaatg
    12601 gtgccggtta tctggtacag cgaagaagag ggagaggaaa taatgctatc ttcgcctccc
    12661 cctataccag aggcggttcc tgacacaaat tctccctccc acagtcttac cattcaaaac
    12721 accgcaacgc cacctgaaca atccccagtt aaaagcaaac gattcactac cttttgggta
    12781 tggttttttt cctgttgtcg ttaggtatct gtgtagcact ggtagcgttt tcaactcttg
    12841 atacacgtct tcctatgagc aaatcgcgta ttttgctcaa tccacgcgat attgacatta
    12901 atatggtaaa taaaagttgt aacagctgga gttccccgta tcagctctct tacgcgatag
    12961 gcgtgggtga tttggtggcg acatcactta acaccttctc cacctttatg gtgcatgaca
    13021 aaatcaacta caacattgat gaaccgagca gttccggtaa aacattatct attgcctttg
    13081 ttaatcagcg ccaataccgt gctcaacaat gctttatgtc ggtaaaatta gtagacaatg
    13141 cagatggttc aaccatgctg gataaacgtt atgtcatcac taacggtaat cagctggcga
    13201 ttcaaaatga tttactggag agtttatcaa aagcgttaaa ccaaccgtgg ccacaacgaa
    13261 tgcaggagac gctccagcaa attttgcctc atcgtggtgc gttattaact aatttttatc
    13321 aggctcatga ttatttactg catggcgatg acaaatcatt gaatcgtgcc agtgaattat
    13381 taggtgagat tgttcaatca tccccagaat ttacctacgc gagagcagaa aaagcattag
    13441 ttgatatcgt gcgccattct caacaccctt tagatgaaaa acaattagca gcactgaaca
    13501 cagaaataga caacattgtt acactgccgg aattgaacaa cctgtccatt atatatcaaa
    13561 taaaagcggt cagtgctctg gtaaaaggta aaatagatga gtcttaccag gcgataaata
    13621 ctggcattga tcttgaaatg tcctggctaa attatgtgtt gcttggcaag gtttatgaaa
    13681 tgaaggggat gaaccgggaa gcagctgatg catatctcac tgcctttaat ttacgcccag
    13741 gggcaaacac cctttactgg attgaaaatg gtattttcca gacttctgtt ccttatgttg
    13801 taccttatct cgacaaattt ctcgcttcag aataagtaac tccgggttga tttatgctct
    13861 ggaatatttg ttgttgagtt tttgtatgtt cccattggta taatatgttg cggcaattta
    13921 tttgccgcat aatttttatt acataaattt aaccagagaa tgtcatgcaa tccattgtaa
    13981 acattgaatg tttatctttt catgatatca acttgcgatc ctgatgtgtt aataaaaaac
    14041 ctcaagttct cacttacaga aacttttgtg ttatttcacc taatctttag gattaatcct
    14101 tttttcgtga gtaatcttat cgccagtttg gtctggtcag gaaatagtta tacatcatga
    14161 cccggactcc aaattcaaaa atgaaattag gagaagagca tgagttctgc caagaagatc
    14221 gggctatttg cctgtaccgg tgttgttgcc ggtaatatga tggggagcgg tattgcatta
    14281 ttacctgcga acctagcaag tatcggtggt attgctatct ggggttggat tatctctatt
    14341 attggtgcaa tgtcgctggc gtatgtatat gcccgactgg caacaaaaaa cccgcaacaa
    14401 ggtggcccaa ttgcttatgc cggagaaatt tctcctgcat ttggttttca gacaggtgtt
    14461 ctttattacc atgctaactg gattggtaac ctagcgattg gtattaccgc tgtatcttat
    14521 ctttccacct tcttcccagt attaaatgat cctgttccgg cgggtatcgc ctgtattgct
    14581 atcgtctggg tatttacctt tgtaaatatg ctcggcggta cctgggtaag ccgtttaacc
    14641 actattggtc tggtgctggt tcttattcct gtggtgatga ctgctattgt tggctggcat
    14701 tggtttgatg cggcaactta tgcagctaac tggaatactg cggataccac tgatggtcat
    14761 gcgatcatta aaagtattct gctctacctg tgggccttcg tgggtgttga gtccgcagca
    14821 gtaagtactg gtatggttaa aaacccgaaa cgtaccgttc cgctggcaac catgctgggt
    14881 actggtttag caggtattgt ttacatcgct gcgactcagg tgctttccgg tatgtatccg
    14941 tcttctgtaa tggcggcttc cggtgctccg tttgcaatca gtgcttcaac tatcctcggt
    15001 aactgggctg cgccgctggt ttctgcattc accgcctttg cgtgcctgac ttctctgggc
    15061 tcctggatga tgttggtagg ccaggcaggt gtacgtgccg ctaacgacgg taacttcccg
    15121 aaagtttatg gtgaagtcga cagcaacggt attccgaaaa aaggtctgct
//
LOCUS       DAZOYS010000095        14997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOYS010000095 DAZOYS010000000
VERSION     DAZOYS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.095"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            180..422
                     /gene="yjbE"
                     /locus_tag="ACHUN9_002530"
     CDS             180..422
                     /gene="yjbE"
                     /locus_tag="ACHUN9_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0695893.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            536..1174
                     /locus_tag="ACHUN9_002531"
     CDS             536..1174
                     /locus_tag="ACHUN9_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0695894.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1171..1908
                     /locus_tag="ACHUN9_002532"
     CDS             1171..1908
                     /locus_tag="ACHUN9_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0695895.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1908..4004
                     /locus_tag="ACHUN9_002533"
     CDS             1908..4004
                     /locus_tag="ACHUN9_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0695896.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4051..4329)
                     /locus_tag="ACHUN9_002534"
     CDS             complement(4051..4329)
                     /locus_tag="ACHUN9_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695897.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4330..4473
                     /locus_tag="ACHUN9_002535"
     CDS             4330..4473
                     /locus_tag="ACHUN9_002535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695898.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4599..5009
                     /gene="psiE"
                     /locus_tag="ACHUN9_002536"
     CDS             4599..5009
                     /gene="psiE"
                     /locus_tag="ACHUN9_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0695899.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHUN9_002537"
     CDS             complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHUN9_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0695900.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6900..7790)
                     /gene="malG"
                     /locus_tag="ACHUN9_002538"
     CDS             complement(6900..7790)
                     /gene="malG"
                     /locus_tag="ACHUN9_002538"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0695901.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7805..9348)
                     /gene="malF"
                     /locus_tag="ACHUN9_002539"
                     /pseudo
     CDS             complement(7805..9348)
                     /gene="malF"
                     /locus_tag="ACHUN9_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            complement(9502..10692)
                     /gene="malE"
                     /locus_tag="ACHUN9_002540"
     CDS             complement(9502..10692)
                     /gene="malE"
                     /locus_tag="ACHUN9_002540"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0695902.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11057..12172
                     /gene="malK"
                     /locus_tag="ACHUN9_002541"
     CDS             11057..12172
                     /gene="malK"
                     /locus_tag="ACHUN9_002541"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0695903.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12244..13584
                     /gene="lamB"
                     /locus_tag="ACHUN9_002542"
     CDS             12244..13584
                     /gene="lamB"
                     /locus_tag="ACHUN9_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0695904.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13736..14656
                     /gene="malM"
                     /locus_tag="ACHUN9_002543"
     CDS             13736..14656
                     /gene="malM"
                     /locus_tag="ACHUN9_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0695905.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14885..>14997
                     /locus_tag="ACHUN9_002544"
     CDS             14885..>14997
                     /locus_tag="ACHUN9_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0695906.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVT"
BASE COUNT         3733 a         3839 c         3927 g         3498 t
ORIGIN      
        1 atgattttta ttgttattta aatataagtt gaaacttata tttgatattc attccaatta
       61 tcctaaaaca ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt tgtgtatact
      121 cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg gaaattgtta
      181 tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact tctgtgtggg
      241 ctgcacctgt acaggtgggc gaagcggcag gggcggcagc aacgtcggtt tcggcgggga
      301 gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa
      361 ccggtggcgg tgatggttct aataccggga ccacaacaac cacaaccacc agtacccagt
      421 aataaagtat gtatccccaa aataattcga gtcattgcat ctgtggcttg aaatatgaag
      481 ggattaacca taaccacact ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa
      541 gcgacctgca ctcattctta tctgcctgtt attacaggcc tgttcagcca cgactaaaga
      601 gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc tgacggacga
      661 tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg ggccgcaatt
      721 gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc
      781 cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg acaacctgat
      841 tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg ttgatggtgc
      901 aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg ccaccgcacg
      961 ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt
     1021 tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc gctactggat
     1081 cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt atttcccggt
     1141 gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt cgcgttgctt
     1201 ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc
     1261 agcagtgagg ccaaaacgct aacgggcgca gagcatttaa tcgatctggt agggcaacct
     1321 cgactggcaa acagctggtg gcctggtgcg gtgattagcg aagagctggc aacggcggca
     1381 gcattgcgtc agcagcaggc gttgctgaca cggctggcag aacagggcgc agattccagc
     1441 accgacgatg ccgccgccat taacgcctta cgccagcaaa tccaggcatt ggaagtgaca
     1501 ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg
     1561 ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc
     1621 gggcttatca gccatcctgg caatcagcca ttcactccag gtcgcgacgt ggcgagctat
     1681 ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg
     1741 gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt agagccgatg
     1801 cccggcagca ttatttatgt tggcctcgca gactccgtct ggagtgagac gcctgatgcc
     1861 cttaacgccg acattcttca gactctgacg cagcggatac cccaataatg aaaaaaagac
     1921 atctgcttag cttactggcg ctgggcatta gcacagcttg ctacggcgaa acatatcctg
     1981 cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg attattacaa actcccaccg
     2041 cgcgtatggc acgggaaggg gagttgagtc tgaactatcg cgataacgat cagtaccgtt
     2101 attactcagc ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgt tacaccgacg
     2161 tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg tataaagata
     2221 aagccttcga tctcaaactg cgtttgtggg aagagagtta ctggctgccg caagtggcgg
     2281 ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc ggaatatctt gttgccagca
     2341 aagcctgggg gccgttcgat tttacgctcg gcctgggctg gggatatctg ggcaccagcg
     2401 gtaatgtgaa aaatccgctc tgttcagcca gtgataaata ttgctatcgc gataacagct
     2461 acaaacaagc gggatctatc gacggcagtc agatgttcca cggcccggcc tcactgtttg
     2521 gcggcgtgga ataccagacg ccctggcaac cgctgcgcct gaaactggag tatgaaggta
     2581 ataattatca gcaggatttt gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg
     2641 cgatttatcg cgttaccgat tgggccgacg ttaaccttag ctatgaacgt ggcaacacct
     2701 ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct gcgcccgtcc tacatcgata
     2761 acgcccgccc gcaatatcaa ccgcagccgc aggatgccat tttgcagcac tcggtggtgg
     2821 cgaatcagtt aacgttgctg aaatacaatg ccggacttgc cgatccgcag atccaggcga
     2881 aaggcgatac gctgtatgtt accggcgagc aggtgaaata tcgtgattcg cgcgaaggga
     2941 tcatccgtgc taatcggatc gtgatgaacg atctgccgga tgggatcaaa acgatccgca
     3001 ttacggaaaa tcgccttaac atgccgcagg tgacgacgga aaccgatgtc gccagcctga
     3061 aaaatcatct cgccggagag ccgttgggcc acgaaacgac gctggcgcaa aaacgcgtcg
     3121 agccagtggt tccgcagtcc accgagcagg gctggtatat cgacaaatca cgctttgatt
     3181 tccatatcga tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc
     3241 agctgggcgt gatgggaacg gcagatttgt ggctgacgga ccatctgctg accatcggca
     3301 gcctgtttgc aaatcttgcc aacaactacg acaagtttaa ctacaccaat ccgccacagg
     3361 actcgcactt accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac gatgcctatg
     3421 tgaataacct gcaagccaac tacttccagc atctgggcaa cggcttctac ggtcaggtct
     3481 atggaggtta tctcgaaacc atgtttggcg gtgcgggggc tgaagtgttg tatcgtccgc
     3541 tggacagcaa ctgggcgttt ggtctggatg ccaactacgt taaacagcgc gactggcgta
     3601 gtgcaaaaga tatgatgaaa ttcaccgact acagcgtgaa aaccggacat ctgaccgcct
     3661 actggacgcc atctttcgcc caggacgtgt tagtgaaagc cagcgtcggg cagtatctgg
     3721 caggggataa aggcggcacg ctggagatcg ccaaacgctt tgatagcggc gtggtggtgg
     3781 gtggctatgc cacgatcacg aatgtttcaa aagaggagta cggcgaaggg gacttcacca
     3841 aaggcgtgta tgtctcggta ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg
     3901 cggcgattgg ctggacgccg ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc
     3961 agttgtatga tatgaccagc gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc
     4021 cagttttcgc aatcactggc accctcattc ttaatggcct gttgagccat caggaacgca
     4081 gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt
     4141 cgatatcggt tgcaggtctg gttgcctgag cgactgggcc atcattgggc tggagacatt
     4201 gctttgcggt aggtcggctt tatcagcagt accgagcgga ccagcataag caggaagaac
     4261 agagactgat aacatcaaag cagcaaaata aggcttcatt tttaccacct ttatcaggtt
     4321 acgtttcatt tgttccagag gaacattgtc gatttttcgc gcattgctgg tggctgggaa
     4381 tcacctgaat gggtgatttt tgaattaccg gcttgggtgc ggtttgtctt gccggatgcg
     4441 ccgccaggcg cggcttatcc ggcctacggg taggtatatc cggctttggt ggaggcgcgc
     4501 tccaaatcca ggttgaacaa aacatacaca aaaaatatag atctccgtca catttttgcg
     4561 ttatacagga agctcgccac tgtgaaggag gtactgctat gacgtcactc tctcgtccgc
     4621 gcgtggagtt tatctccacc attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg
     4681 gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca tctggcagat gtgctgttcg
     4741 cgccagaaca aaccagcaaa tatgagctgg tagaaggact ggtggtttac tttctctatt
     4801 tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt
     4861 actttgtcta tattgggatc accgccattg tgcgcttgat catcgtcgat cataaatcgc
     4921 cactggatgt gttgatctac tcggctgcga tcctgttact ggtgatcact ctgtggctgt
     4981 gtaattcgaa acggctgaag cgggagtaaa aagaaagcac gccgaactgg cgcggcgtgc
     5041 tggacaggaa gattacagcg tagcagtttg ttgtgttttc ttcgtttccg gttcccagag
     5101 ggcttccagc tcctcaaggg ttttaccttt ggtttccggg acaaatttcc acataaatag
     5161 tgctgccaga acgcccatac acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc
     5221 caccagccag gagtttttgt ccatcatcgg gaaggtccag gagacgaagt agttcgccag
     5281 ccactgggcc gccaccgcga ttgccagcgc tttaccacga atagcattcg ggaagatttc
     5341 cgacagcagt acccagcata ccggacccca ggacatggca aaggcagcaa catagaacag
     5401 catcgacagt agcgccacaa tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa
     5461 cataccgatt gccattccga gtgcgccgat aatttgcagt ggcttacgac caaatttatc
     5521 caccgtcata attgccagaa cggtgaaggt gaggttgata actccgacaa taatggtctg
     5581 caacagcgcg atatccgtgc tggcccccag cgttttgaac acttccggcg cgtagtacag
     5641 caccacattg atgccgacaa attgctggaa gatggagagc ataacgccga tgacaatcac
     5701 gcccacgcca aacatcagca gacgaccacc ggttttgcgg ccatgatcca gggagtgttt
     5761 aatttcctgt actgcctgag ttgcaagcgt gttgcccata attttgcgca ggatactttc
     5821 cgcctgttct tgcttgccgc gtgacatcag ccagcgagga ctttctggca cggtatacag
     5881 cagcattaag aacagcagtg cagggataca ttccgaggca aacatataac gccagccgtc
     5941 agtattcagc cagctggcat caccggaacg ggcaataaaa tagtttacgc agtaaactaa
     6001 aagttgcccg aaaataatcg caaactggtt aaatgaaacc agttttccgc gaatatgagc
     6061 tggagccagt tccgcaatat acattggtga aagcattgag gctaaaccaa cgccaatacc
     6121 gccaataata cgataaataa caaattccgg gacataacct gccagataaa taggtactgt
     6181 gttgtccggg tttatagagg taaaaccaag ttctggccag gcagaaccta caccagaaat
     6241 aaaaaacagg acagcagcaa tcttaagtga atcacgacgg ccgaagcggt tactgcaata
     6301 accaccgagg gcaccgccga tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa
     6361 cagggagttg gcagcggatt cacttaagtt ttgtggagca acaaagacgg tattgagtga
     6421 ctcaacagta ccggaaataa cggcggtgtc gtagccaaat aataaaccac ctaatgtagc
     6481 gactaaggta atcgaaaata tataactgga attatactgg gtattcattc agacctgcct
     6541 tagaccattc tgatctttta tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa
     6601 ttatcatttt ttacaatgcg tttacgtaat gtcttttctg tgatcttaat tgtgataatt
     6661 atcaaaaatt gatgaaaaca atactattgc cgtgactcag agcacgaaag agaattatcg
     6721 taagtgggaa aacaaataac gtaaaaataa taagctctat gatggaaata ttaaccggcg
     6781 aacgattcag attgcagacg aaagaaaaaa aggcgctccg tggagcgccg aataacagtc
     6841 acaagttggg ataacgtaag ttgagggtgc agcggcataa cattggcaga acaacatctt
     6901 taacctttca caccacctgc cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag
     6961 acgatggtga tcggtaatgc agacatcacg gcagcggcgg caaagtcacc ccacaggtag
     7021 ttttgcgggt tgaggtattg ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc
     7081 agtaacagcg acgcgaccgg aacttcagta atggcagcga taaacgacag gatgaatacc
     7141 accgccagaa tcggtactga cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc
     7201 gcaccatcca gcgcagccgc ttcttccagc gaactatcga tggtttcgaa atagcctttg
     7261 atggtccaga catgcagcgc aatcccaccc agatacgcga aaattacgcc gccgtgagta
     7321 ttcaggccaa tgaatggaat gtactcaccc agacgatcaa acaacgcata caacgcgacc
     7381 agtgaaagta ctgccgggaa catctggaaa atcagcattc ctttcagcag cgtcgctttg
     7441 cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg tagagagcgc gacaatgcca
     7501 atcgcggaaa tcccggcgac ctttaccgag ttccatagcc acagcagtac cgggaatggc
     7561 ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac ctaaagccag tttccagtga
     7621 tcccaggaga tttgctccgg gatcaggctg ccgatcgcaa agttcccctg gcgcagtgag
     7681 atagcgacga ccatcagcag cgggaacata atcgccgcga taaaaagtag cagtagcagg
     7741 tgagtaataa acaaacgtgc tttttgcgat ttcggttgga ccattgccat ttttgttatc
     7801 tcccttaatc aaacttcatt cgcgtggctt tcaggttcac tatcgccagc gcacccacca
     7861 gcaggaagag cagcgggcaa ttgctgcagc cagaccgaag tcctgacccc cgccgccttc
     7921 aaaagcgata cggtaggtgt agttaacaag caggtcggta taaccggctg gcgtggtcgt
     7981 gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt tgttaaagtt
     8041 aaaggcgaag ctggcgatca tcagcggcgt cagcggttta atcagcagcg gcagcgtaat
     8101 cttaaagaag ttctggaacg gacctgcgcc atccattgct gaggcttcat acaaatcgtc
     8161 cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac ccagccaggt
     8221 attgacgata attagcatcg tgcgggcggt ggtcggatcg ctgaaccagg cgggcttcac
     8281 gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag ctctggttaa acaacccttt
     8341 gaaaatcaag attgaaatga atgatggcac cgcgtagggc agaatcagca gaacgcgata
     8401 gaccgctttg ccgcgcaatg cttcccactg caccagacat gccagaacca tgccgaccgc
     8461 caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa
     8521 cggtttctga atgccttcgt cggtgaagac gcgggtaaag tttttccagc cagtagtcac
     8581 ggtgtaaccg gggcttagct tttcatcacc ccagttgccg tcggcggtaa tggactggta
     8641 aaagccaatt tggttattcg gacgatattt cacgccgctc tgattattcg tcaacgtgcc
     8701 gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg agctcatcat
     8761 cactttgttg ccatccggca gaatggcggt aatgtcactc agcgcctgac gattctgggt
     8821 aatcacgcgt agattcgcgc gttcgccttc gggctgggcg gtcgtttctt tcagttgcag
     8881 tttttgttcg ccgccaaatt taaacgcgtc ggagaggtaa tttttgccga tttcgccgtc
     8941 actgagcgcc agttgccact catcgcccgc cgggtaaaga ccaaagttat aggttttgcc
     9001 tgcttgccag gagcgatcta acaacacttc ctgcgcacat tcaaaagtca gctggttagt
     9061 gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga ggacgaataa
     9121 tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac gattggcgaa
     9181 aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt attccccttg
     9241 tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta gcactgacca
     9301 tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg ggttcttcct
     9361 cattccagga cggataaggc tttcacgcct tatccgacaa caactgcctg atgcgacact
     9421 gatgcgtctt atcaggccta catgcgtttc agttttgtag gccggacaag gcgttcacgc
     9481 cgcatccggc atttcacagc attacttggt gatacgagtc tgcgcgtctt tcagggcttc
     9541 atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
     9601 agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt ccatggtggc
     9661 ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcca ctgcacccag
     9721 cggtttgtct ttattgaccg cttccagacc ttcgtcagtc agcagatagt tttcgaggaa
     9781 ctctttcgcc agctctttgt tcggactggc ggcgttaata ccggcgctca gcacgccaac
     9841 gaacggttta gatggttgac ccttgaaggt cggcagtacc gttacaccat aattcacttt
     9901 gctggtgtcg atgttggacc atgcccacgg gccgttgatg gtcatcgctg tttcgccttt
     9961 attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat
    10021 aaggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgg ccacgtcttt
    10081 aatatcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagcag caatcagcgg
    10141 ccaggtgaag tacggttctt gcaggttgaa catcagcgcg ctcttacctt tcgctttcag
    10201 ttctttatcc agcgccggga tctcttccca ggtttttggt gggttcggca gcagatcttt
    10261 gttataaatc agcgataacg cttcaacagc gatcgggtaa gcaatcagct tgccgttgta
    10321 acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt ccggggtgat
    10381 ttcagccaac aggccagatt gagcgtagcc accaaaacgg tcgtgcgccc agaagataat
    10441 gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt tatccggatg
    10501 ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt cagcgagacc
    10561 gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga ttttggcgag
    10621 agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt
    10681 ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga
    10741 ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc cccaccgttg
    10801 ctttgtgtga tctctgttac agaattggcg gtaatgtgga gatgcgcaca taaaatcgcc
    10861 acgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag ttcacagaag
    10921 ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg aggatttaag
    10981 ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg acaggttgtt
    11041 acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga
    11101 ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt
    11161 cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc ttgagacgat
    11221 caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc cagcagaacg
    11281 cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag tagcagaaaa
    11341 catgtcattt ggcctgaaac tggctggcgc aaaaaaagag gtgattaacc aacgcgtcaa
    11401 ccaggtggcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga aagcgctctc
    11461 cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtggccgagc caagcgtatt
    11521 tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga
    11581 aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc acgatcaggt
    11641 cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gccggtcgcg tggcgcaggt
    11701 tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg gatttatcgg
    11761 ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc accgcaatcg atcaagtgca
    11821 ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccagttgaaa gccgtgatgt
    11881 ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc cgagtgatat
    11941 cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca acgaaaccca
    12001 aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga acgacgtggt
    12061 gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt gccatctgtt
    12121 ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt aagcacccca
    12181 caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat
    12241 agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg
    12301 tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat tggctggaca
    12361 ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa ataccgtctt
    12421 ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg gaaagagggc
    12481 gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca gaatgactgg
    12541 gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg
    12601 ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc aacgtcatga cgttcatatg
    12661 atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa catcgatgtt
    12721 ggcttcggta aactctctct ggcagcaacc cgctcctctg aagctggtgg ttcttcctct
    12781 tttgccagca acaatattta tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg
    12841 cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt cgactacggt
    12901 cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga cggctggtta
    12961 ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt tgttcagtac
    13021 gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt
    13081 gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg tatcctcgac
    13141 cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat gtaccaggat
    13201 atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg cccgatgtac
    13261 aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt cgaatcccag
    13321 cgcaccggcg acaagaacaa tcagtacaaa attaccctcg cacaacaatg gcaggctggc
    13381 gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag
    13441 aaatggggtt acgactacaa cggcgatagc aaggttaacc cgaactacgg caaagccgtt
    13501 cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt
    13561 gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggcg tttacgccgc
    13621 atccggcata aaaacaggtt gtcattatct gaaaggggcg aaagcccctc tgattatcgg
    13681 gtttagcgcg ctattgcctg gctaccgctg agctccagat tttgaggtga aaacaatgaa
    13741 aatgaataaa agtctcatcg ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg
    13801 aattagcctt gccgatgtta actacgtacc gcaaaacacc agcgacgcgc cagccattcc
    13861 atctgccgcg ctgcaacaac tcacctggac accggtcgat caatctaaaa cccagaccac
    13921 ccaactggcg accggcggcc aacaactgaa cgttcccggc atcagtggtc cggttgctgc
    13981 gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa
    14041 acaaaccagc gtttttgcgc cgaacgtgct gattcttgat cagaacatga ccccatcagc
    14101 cttcttcccc agcagttatt tcacctacca ggaaccaggc gtgatgagtg cagatcggct
    14161 ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa aaactttatg ttctggtctt
    14221 taccacggaa aaagatctcc agcagacgac ccaactgctc gacccggcta aagcctatgc
    14281 caaaggcgtc ggtaactcga tcccggatat tcccgatccg gttgctcgtc ataccagcga
    14341 tggcttactg aaactgaaag tcaaaacgaa ctccagctcc agcgtgttgg taggaccact
    14401 atttggttct tccgctccag ctccggttac ggtaggcaac acggcggcac cagctgtggc
    14461 tgcacctgct ccggtgccgg tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta
    14521 ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg cgttaaaact
    14581 gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt tccaccttta tcagcagtgt
    14641 aaaaggcaag gggtaattac gccccacagt gctgattttg caacaactgg tgcgtctcct
    14701 ggcgcacctt tttttatgct tccttcctgg gatatgagcg attttttata gtacttcact
    14761 tcttcttcac taagaatgtc cattatctca atgccttatc agagattctt ttcctttcgc
    14821 cggtagtgtc tggacgctca ggctactttt ccaggttatt ttatttctgt attacagagg
    14881 ttttatgata ggtcatatca caaattctgg cggcaataac tctttgatga aacatgatgt
    14941 ggttcaagga aataatatag tagatcttga tttactacgt aatttcaatg gggtgac
//
LOCUS       DAZOYS010000096        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOYS010000096 DAZOYS010000000
VERSION     DAZOYS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.096"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUN9_002545"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUN9_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0695907.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHUN9_002546"
     CDS             complement(1556..1879)
                     /locus_tag="ACHUN9_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0695908.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUN9_002547"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUN9_002547"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0695909.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUN9_002548"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUN9_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0695910.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUN9_002549"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUN9_002549"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0695911.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUN9_002550"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUN9_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0695912.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHUN9_002551"
     CDS             7479..8516
                     /locus_tag="ACHUN9_002551"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0695913.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUN9_002552"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUN9_002552"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0695914.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUN9_002553"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUN9_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0695915.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUN9_002554"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUN9_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0695916.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHUN9_002555"
     CDS             10687..11568
                     /locus_tag="ACHUN9_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0695917.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUN9_002556"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUN9_002556"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0695918.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUN9_002557"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUN9_002557"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0695919.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHUN9_002558"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHUN9_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0695920.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZOYS010000097        14577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOYS010000097 DAZOYS010000000
VERSION     DAZOYS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.097"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            78..620
                     /locus_tag="ACHUN9_002559"
     CDS             78..620
                     /locus_tag="ACHUN9_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0695921.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            720..1922
                     /locus_tag="ACHUN9_002560"
     CDS             720..1922
                     /locus_tag="ACHUN9_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0695922.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2142..2924
                     /locus_tag="ACHUN9_002561"
     CDS             2142..2924
                     /locus_tag="ACHUN9_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0695923.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2939..4144
                     /gene="rhmD"
                     /locus_tag="ACHUN9_002562"
     CDS             2939..4144
                     /gene="rhmD"
                     /locus_tag="ACHUN9_002562"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR0695924.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4201..5490
                     /locus_tag="ACHUN9_002563"
     CDS             4201..5490
                     /locus_tag="ACHUN9_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0695925.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5508..6311
                     /gene="yfaU"
                     /locus_tag="ACHUN9_002564"
     CDS             5508..6311
                     /gene="yfaU"
                     /locus_tag="ACHUN9_002564"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0695926.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6352..7254)
                     /locus_tag="ACHUN9_002565"
     CDS             complement(6352..7254)
                     /locus_tag="ACHUN9_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0695927.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7447..8637)
                     /gene="glpC"
                     /locus_tag="ACHUN9_002566"
     CDS             complement(7447..8637)
                     /gene="glpC"
                     /locus_tag="ACHUN9_002566"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0695928.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8634..9893)
                     /gene="glpB"
                     /locus_tag="ACHUN9_002567"
     CDS             complement(8634..9893)
                     /gene="glpB"
                     /locus_tag="ACHUN9_002567"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0695929.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9883..11511)
                     /gene="glpA"
                     /locus_tag="ACHUN9_002568"
     CDS             complement(9883..11511)
                     /gene="glpA"
                     /locus_tag="ACHUN9_002568"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0695930.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11784..13142
                     /gene="glpT"
                     /locus_tag="ACHUN9_002569"
     CDS             11784..13142
                     /gene="glpT"
                     /locus_tag="ACHUN9_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0695931.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13147..14223
                     /gene="glpQ"
                     /locus_tag="ACHUN9_002570"
     CDS             13147..14223
                     /gene="glpQ"
                     /locus_tag="ACHUN9_002570"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0695932.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14265..14507)
                     /locus_tag="ACHUN9_002571"
                     /pseudo
     CDS             complement(14265..14507)
                     /locus_tag="ACHUN9_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         3330 a         3589 c         4014 g         3644 t
ORIGIN      
        1 cattgaatcc tctgtgccag gcagtacaat cccgcagctt tgggaatttt gattaattgc
       61 ttgtgggaaa ggggataatg aaaaaaattg cgctagcagg tctggccgga atgctgttgg
      121 tttctgcatc ggtcaatgca atgagcatca gcggtcaggc gggtaaagaa tacaccaata
      181 ttggtgtcgg ttttgggact gaatcgacgg gcctggcgtt aagcggtaac tggacacata
      241 acgacgacga cggtgacgtc gctggcgtgg ggctggggtt gaatctgcct ctcgggccgt
      301 taatggcgac cgttggcgga aaaggcgttt acaccaaccc gaattacggc gatgaaggtt
      361 atgccgcagc ggtaggaggt ggtttgcagt ggaaaattgg caacagcttc cgtttgtttg
      421 gcgagtatta ctactctccg gattcactct ccagcggtat taaaagttat gaggaagcga
      481 atgctggcgc gcgttacacc attatgcatc cagtcagtat tgaggcgggt tatcgctacc
      541 tgaatctgtc gggtaaagac ggtaaccgcg acaacgccgt ggctgacggc ccgtatgttg
      601 gggttaacgc cagtttctga tcccttccgg cgcggcattc tgtcgcgcca ttcagtttct
      661 tactacgcct tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca
      721 tgttaaaagt ggaaatgtta tccaccgggg atgaagtgtt acacgggcaa atcgttgaca
      781 ctaacgctgc ctggctggcc gattttttct ttcatcaggg gttgccatta tctcgccgca
      841 atacggtggg ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg
      901 ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag tgatgattta agtgcactcg
      961 ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg
     1021 aacgctattt ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc
     1081 tgcctgccag tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc
     1141 ggcttaatcg ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg
     1201 tcgagcacga aatcctgccg cgcctgcgcg agcgtttttc tttaccacag tcgccggttt
     1261 gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc
     1321 tacaactgcc gccgggtgta acaatgggct atcgctcctc aatgccgatc atcgaactga
     1381 aactcaccgg accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac
     1441 gtgttgccgg acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc
     1501 gcgaattgca aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat
     1561 tggctttgca actttctcgc gcaggtgctc cattgctggc gtgtgaagtg gttccttcac
     1621 aggaagaaac cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg
     1681 ccgggctggc actggctgtt tcgggtttcg agaacgagca tctcaacttt gcgctagcca
     1741 cgccagacgg cactttcgct ctgcgtgtgc gtttcagcac tacacgctac agcctggcta
     1801 tccgtcagga agtgtgcgca atgatggcac tgaatatgct gcgccgttgg ttaaacggcc
     1861 aggatatcgc cagtgagcat ggctggattg aggttgttga gtccatgacc ttatctgtct
     1921 gattccgcca ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaacttgt
     1981 ccaacctgtt gtactcaata agaaaaaatc cgtgcactga ttcacaaatt tgtgcgtaat
     2041 ttctccagtc cgctggacga ggagcgggat ttgatgcgct tcacaatttt actccccctg
     2101 agcaacgaga ctgattccag tatctaagac tggagtccag tatgttggaa tcaagcaaag
     2161 ttcccgccct gacacgggct attgatattc tcaatttgat tgcccgcatt ggcccctgta
     2221 gtgctgcgac gatcattgac acattgggaa tccctaaaag cacggcctat ttgctgctta
     2281 atgaactcag gcgtcagcgt tttcttagcc ttgatcacca ggaaaatttt tgtttgtgga
     2341 ccaggctggt ggagctttct ggtcatgcgt taagcaagat ggatctccgg gaactggctc
     2401 gaccgcgttt gacgcaactg atggatacca ccgggctgtt atgtcacctg gggattattg
     2461 ataacggcag cgcttattac attttaaaag tggagtcatc agccactatc agcgtgcgtt
     2521 cccatgaagg aaaaagcctt tcgctttatc gctccggaat aggtaaatgc ctgcttgcct
     2581 ggcaacctgc agcggtacag caaagtatta tcgaagggct ggtctgggag caggcgacac
     2641 ccacaacgat tactcatcca cagcaacttc acgaagaact ggcgcgtatt cgccgtcagg
     2701 gctggagcta tgacaacggc gaagattacc ccgatgtccg ttgtgtcgct gcgccggtgt
     2761 ttaacgccaa taacgagctt accgctgcga tctcggtggt cggtacccgc ttacaaatca
     2821 atgaagaata ccgtgattat ctggcgggca aggccattgc ctgcgccagg gatatttcac
     2881 gtctgttggg atggaaaagt ccctttgact tacaggcctc ataacaatgg agaacatcat
     2941 gaccctacca aaaatcaaac aggttcgcgc ctggtttact ggcggtgcga cagcagaaaa
     3001 aggtgctggc ggcggtgatt atcacgacca gggggcgaat cactggattg acgatcatat
     3061 tgccaccccg atgagtaaat accgcgatta cgagcagtca cgccagtcat ttggcattaa
     3121 cgttcttggc acgttgattg ttgaagtcga agcagaaaac ggccagaccg gattcgctgt
     3181 ttcgacagcc ggtgaaatgg gctgttttat tgtcgaaaaa catcttaacc gtttcattga
     3241 gggtaaatgt gtcagtgata tcaaactgat ccatgatcaa atgctcaatg ccaccctgta
     3301 ttactccggc tctggtggcc tggtgatgaa cacgatttcc tgtgtcgatc tggctctgtg
     3361 ggatctgttc ggcaaagtgg tcgggcttcc ggtttataaa cttttaggcg gcgcagttcg
     3421 tgatgagatt cagttctacg ccacaggcgc gcgtccggat ctggcaaaag agatgggctt
     3481 tatcggtggc aaaatgccga cgcactgggg gccacatgat ggcgatgcgg ggatccgcaa
     3541 agatgccgtt atggtcgcgg atatgcgtga aaaatgcggt gaggatttct ggttaatgct
     3601 cgactgctgg atgagtcagg acgtgaacta tgccaccaaa ctggcccacg cttgcgcgcc
     3661 ctataacctg aaatggatcg aagagtgcct gccgccacag cagtatgaag gttatcgcga
     3721 actgaaacgc aacgcgccag tcggaatgat ggtcaccagc ggtgagcacc acggcacact
     3781 gcaatctttt cgtacgcttt cagaaaccgg tatcgacatt atgcagccgg atgttggctg
     3841 gtgcggtggt ttaaccacgc tggtggaaat tgccgcaatc gccaaatcca gggggcaact
     3901 ggtggtgccg cacggttcgt ctgtttactc tcaccatgcg gtgatcacct tcaccaatac
     3961 gccattcagc gaattcctga tgaccagccc ggattgttca acgatgcgtc cgcagtttga
     4021 cccgattctg ctcaatgagc cggttccggt gaatggtcgt attcataaat cagtgcttga
     4081 taaacccggt tttggcgtcg aactcaatcg tgactgcaat ttgaaacgcc cctacagcca
     4141 ctaatcactc cgggcgttgc tgtgacaacg gtaacgccca catattctaa ggagcttttt
     4201 atgagcaccg ctttgcttga cgccgtggtg aagaaaaacc gtgtgcgttt aattccgttt
     4261 atgttggcgc tgtatgtgct ggcgtttctc gaccgttcga atatcggttt tgccaaacag
     4321 acctaccaga ttgataccgg gttgagtaat gaagcttatg cgctgggagc aggcattttc
     4381 tttgtggtat atgcgtttct gggtgttccg gcgaatcttt tgatgcgcaa actgggggcc
     4441 agaacctgga ttggtacgac aacactgctg tggggatttc tttcggcagc catggcatgg
     4501 gccgatactg aagcgaaatt tctgatagtt cgcactctgc ttggtgctgc ggaggccgga
     4561 tttttccctg gtatgattta tctcacctcg caatggtttc cgcagcgtaa tcgcgccagc
     4621 attatggggc tgttctatat gggcgcaccg ctggcgttaa cactgggatc accgctttcc
     4681 ggcgcgctgt tggagatgca tggatttatg gggcatcccg gctggttctg gatgtttgtg
     4741 attgaaggat tgttggcagt tggtgctggg gtattcacat tcttttggct tgatgacaca
     4801 ccggagcagg cacgttttct gagtaaacaa gaaaaaacgt tgcttatcaa tcaactggca
     4861 agtgaagaac aacagaaagt gacttctcgg ctgagcgatg cgctgcgtaa tggccgagtc
     4921 tggcaactag cgattatcta cctgaccatt caggtggcgg tttacggatt aattttcttc
     4981 ctgccgaccc aggttgcggc attgctggga acaaaagtgg gctttacagc gtcggtggtc
     5041 accgccattc cgtgggttgc ggccttgttt gggacctggc ttattccgcg ctattccgat
     5101 aaaaccggcg aacggcgtaa tgtcgcagcg ctgacattac tggcggcggg cattggtatt
     5161 ggtctgtccg ggctgctttc tccagtaatg gcgatcgtag cgctgtgtgt tgcagctatc
     5221 gggtttattg ccgtgcagcc ggtgttctgg acgatgccga cacaactgct ttccggtacg
     5281 gcgctggctg cggggattgg ttttgtaaac ctgtttggtg cagtgggcgg gtttattgcc
     5341 ccgatcctgc gcgtgaaagc agaaacgtta tttgccagcg atgcggcagg attactgaca
     5401 ctggcagcgg tggcggtcat cggttcgctg attattttca ctctgcgtgt aaatcgtact
     5461 gttgcgcaga ccgacgtggc acatcattaa ataggttaag gaacacgatg aacgcattat
     5521 taaccaatcc ctttaaagaa cgtttacgca agggcgaagt gcaaattggt ctgtggttaa
     5581 gctcgactac ttcttatatg gcagaaattg ccgctacttc cggttatgac tggttgctga
     5641 ttgacgggga gcacgcgcca aacaccattc aggatcttta tcaccagcta caggcggtag
     5701 cgccctatgc cagccaaccc gtgatccgtc cggtggaagg cagtaaatcg ctgattaaac
     5761 aagttctgga tattggcgcg caaactctat tgatcccgat ggtcgatact gccgatcagg
     5821 ctcgtcaggt ggcgtctgcc acgcgctatc ctccctacgg tgagcgtggt gttggggcca
     5881 gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta catggcgcaa gtcaacgatt
     5941 cgctttgtct gttagtgcag gtggaaagta aaacggcact ggataacctg gacgagatcc
     6001 tcgacgtcga agggattgat ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg
     6061 gctacccgga taacgccggg cacccggaag tgcagcgaat tattgaaacc agcattcggc
     6121 ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt ggctcctgat atggcgcagc
     6181 aatgcctggc gtggggagcg aactttgtcg ctgttggcgt tgacacgatg ctctacagcg
     6241 atgccctgga tcaacgactg gcgatgttta aatcaggcaa aaatgggcca cgcataaaag
     6301 gtagttattg atatcaaagg cccatgggga tcggctgtgg gcctgtgtta attagtggtt
     6361 attcgctgcc agatcggctt cgcttagctg ggtggccgcg agcacctggt cacgatcaat
     6421 gccctgttcc agcatgcgca aagcaatttt aatggcttgt tcatgcatac cttcctgcca
     6481 gccaatttgg tgtccttcct gccgtagccg ctcagcaata gtcatcaatc tctccttttg
     6541 tagtggtgaa cgttcggaaa tctcctcaat aaaacgggtg aaacgggagg tatcgccgca
     6601 ttgcagcaga taattaaaca gtgtttgtag ctggctgtca ttagtgaagc ctttaaccaa
     6661 aagcgtggtg atcctgtcga ccataccgat taaatcgcgg tcgcgaatat gcttttgaat
     6721 cagttcaagc agagctatac gccgatgttg catgatctca tcgtcaggta cgatggtgat
     6781 atccaccaac ggaaaagctt cggtgtacaa ctgccgggca agttgcggat cgtcaaactc
     6841 atccagccag ttaagtgagt acgggtaggg cgatgtttcg ccatgataaa acagcaatgg
     6901 caccaccagc ggaactctgt catagccttt atccaggtga cgctgcatgg cggcagtggc
     6961 atagcgcatt agccgaaaag ccatattctt ttctgcagag ctttgatgtt caatcacgca
     7021 gtagatatag ccgtcaccgt cgctggtttc cacggaccac aaaacatccg agtagtaagc
     7081 gcgtaaactt ttttcaataa aactagtggg ttccaggcgt aaggtttgca ggttgcaaag
     7141 cttgcgcagt ggttctggca aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa
     7201 cataaaggtt ttaaataccg catcatgcgg ggaggaggtt gttgattcgg tcatggttgt
     7261 ccgtcacctg atatttatga tgacgcgaca ttacggttat cccaggcctt tgacatctgc
     7321 cagtttcagc ctttgcgaga cgttttcaga ggaattttca ctgcgttgca acgcatacat
     7381 tgggcacgga atcgtgtacg caaaaatgcc cggtaaccac atgcttaccg ggcatcagaa
     7441 aggagtttaa gccagcgcct gggccagtag cgtaatcgga tgttcgcagc gaagacttgt
     7501 ggacatctca atctgccatt tacaggtttc gcagtcggtg accaccagat ctgcgccgct
     7561 ttcttctatc tggcggaaca gtggtgcgcc gatggcttgt gaggtggggt agttctcttt
     7621 tttgaaaccg taagtacccg caataccgca gcactgggaa tccagcaccg ttaactcaag
     7681 ccccgggatt ttacgcaaca gctccagggt gtagagcgtc cagcccattt tttccatatg
     7741 gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg ttttgccttc
     7801 gtccagcttg cgccacagcc agcgggttgc cagttcgata tgatcgcgca agcctttgtt
     7861 gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt
     7921 ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta cattcgtaat
     7981 tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt agcggtacgc cgcagcattt
     8041 ttctttgctg agcagttgta caccgatacc cattgcgttg agcactttaa ttaaatcttt
     8101 acctaactgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga cctggtcttt
     8161 atattgtgct tgctgagccg ctatgctgcg ataccagcga cggaacgtgc cgaaggagta
     8221 tttcggtagc gtgcgacgat gatcgatttt taacgccgca tcaagcagct gccgcaccgg
     8281 tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc tacccatcag
     8341 gtcggtatga ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat attttgcccg
     8401 cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt cacaacgttt
     8461 gcagttgatg caatatttca gcgcctcgtc atacagtgcg ccatctttca aacgcagacg
     8521 ctcgccatcc ggcccggctt gttttggccc tggataaccg ggattcaccc ggctcaccgg
     8581 gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt cgttcattgt
     8641 tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcactgac ggcacaaaca
     8701 ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc gatggcaaac
     8761 aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg
     8821 aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg
     8881 gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt
     8941 ccgccactaa agaaactgcc gctggcgaga accgcgaaac gtggacgtag cggaatatcg
     9001 gcgtgattgc gggtccagat ttcattcact acgccatttt tacaggtcac ttttttcact
     9061 tcatcgcccg gcatccacac gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc
     9121 agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcagtga acaaggtagt
     9181 ttttcattca accaacgcca cagtttgtca tcggccaggc cgaagcaggc gggcatcagg
     9241 atcatttcgc aggtattggc gacaggaata agcgcatcaa gtaacagcgg ccagttttct
     9301 tcattatcaa ggaaacgggc gatattcacc gcgcgaaatt cggtggcgtt attgcgcagg
     9361 acatccagtt ccggcagctc tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac
     9421 gaagccgccg caagatgcgc ctggaaatcc atcagcccgc ttatccccac tacacatatc
     9481 ttcttcgcgg gtagcggcca gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa
     9541 gtgccgagcg gggtaacgcg ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg
     9601 ctttcagcga tcaatgcctg cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga
     9661 agactgtaag gatgggctgg tgcctgctga cgcaaagatt ccagtccact gtgaatgtct
     9721 gtcaccggtt gaccatcagg cagatggctc agcaaatcca gcgaaccgga tgagaaatgc
     9781 agcgcgcttt gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg cagttgcagg
     9841 ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg catctttctg
     9901 ctccttctcc agaccacaca gtccctgata aacccagcgg gtaaattcgc tttcgcgcag
     9961 tgcatctccc caggcgatgg gttgcacgcc tttccagcgt tcgttaagga aggtggaaag
    10021 ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt tgcagcagtc cggcagcgcg
    10081 gcaggcgcag agttcgccct ggcaggtacc catccccaca cgggtacgac ggcgtaaatc
    10141 cagcaggcta ttaacgttta aattttctac cgcgtactgc acttcacccg ctgttaccgc
    10201 ttcgcactca cataccaggc tacggtgcag acgaccttcg ctcagccagg caggcgtgcg
    10261 atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga tgactttacg
    10321 caaggtaact tcagcgggtt cctgtgaacc aggaagtgcc agatcggcgg tcgtacaggg
    10381 gcgcgtgttg cccagtttgc ggcacacggc gtcggtagcc cattcagcca tcagccgata
    10441 ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc gttcagcatg
    10501 gtcgagcagc acgatgccac ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac
    10561 cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag
    10621 tttttcccct tcacgcagca gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat
    10681 ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc gaaatggtat cgccaggcac
    10741 cagaatatcg gcgtcggaag gtttacggca gcggttgatc acatgctggt taatgcggtg
    10801 atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat cggcatattc
    10861 ggcaatgtgt tgcccccaga tcccggcggc attaaccacg acaggcgcat gaagggcctg
    10921 agtttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc cttcacgaat
    10981 cagtcccgtg acttcatgag cggtaaggat aacggcaccg tgttctttgg catccagcat
    11041 gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc ggaactttca ccgcgccaat
    11101 caatgccggg ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc
    11161 gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc tggaaggaga ggtcatcttc
    11221 cggcagggta ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag
    11281 gatctggttt tcactaatgc attcgcgggc cgattccgca tcggttaccg catagcgcgc
    11341 accgctgtgt agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc
    11401 aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc
    11461 gccgccgcca atgataatca cgtcacttga ttgcgagtcg cgagttttca ttgttatccc
    11521 tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt gatttcgcgc
    11581 atattcgctc atgattcgaa agtgaaacgt gatttcatgc gtcattttga acattttgta
    11641 aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt ttcttaacat
    11701 cgcggcaact caagaaacgg caggttctct cactgaatca ggctgttaat cataaataag
    11761 accacgggcc acggaggcta tcaatgttga gtatttttaa accagcgcca cacaaagcgc
    11821 gcttacctgc cgcggagatc gatccgactt atcgtcgatt gcgctggcaa attttcctgg
    11881 ggatattctt tggctatgcg gcttactatt tggttcgtaa gaactttgcg cttgctatgc
    11941 cttatctggt tgagcaggga ttctcacgcg gtgatttagg ttttgccctt tcggggatct
    12001 cgattgctta tggattttcg aaattcatca tgggttcggt atcggatcgc tcgaatccgc
    12061 gcgttttcct gcccgcaggt ttgattctgg cggcggcagt gatgttgttt atgggctttg
    12121 tgccatgggc gacgtcgagc attgcggtga tgtttgtact gttgttcctc tgcggttggt
    12181 tccaggggat ggggtggccg ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag
    12241 aacgtggcgg cattgtgtca gtgtggaact gtgcgcacaa cgtcggtggt ggtattccgc
    12301 cgctgctgtt cctgctgggg atggcctggt tcaatgactg gcatgcggcg ctctatatgc
    12361 ctgctttctg cgccattctg gtggcattat tcgcctttgc gatgatgcgc gataccccgc
    12421 aatcctgtgg cttgccgccg atcgaagagt acaaaaatga ttatccggac gactataacg
    12481 aaaaagcgga acaggagctg acggcgaagc aaatcttcat gcagtacgta ctgccgaaca
    12541 aactgctgtg gtatatcgcc atcgccaacg tgttcgttta tctgctgcgt tacggcatcc
    12601 tcgactggtc accgacttat ctgaaagagg ttaagcattt cgcgctggat aaatcctcct
    12661 gggcctactt cctttatgaa tatgcaggta ttccgggcac tctgctgtgc ggctggatgt
    12721 cggataaagt cttccgtggc aaccgtgggg caaccggcgt tttctttatg acactggtga
    12781 ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa cccaaccgtc gatatgattt
    12841 gtatgattgt tatcggcttc ctgatctacg gtcctgtgat gctgatcggt ctgcatgcgc
    12901 tggaactggc accgaaaaaa gcggcaggta cggcagcggg ctttaccggg ctgtttggtt
    12961 acctgggcgg ttcggtggcg gcgagcgcga ttgttggcta caccgtggac ttcttcggct
    13021 gggatggcgg ctttatggta atgattggcg gcagcattct ggcggttatc ttgttgattg
    13081 ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact gcaaaaacgc aacggaggct
    13141 aatggcatga aattgaagct gaaaaacctt agcatggcga tcatgatgag cactatagtc
    13201 atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt
    13261 gccagtggat atttgccgga gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga
    13321 gcggattatc tggaacagga tttggtgatg accaaagacg accatctggt tgttctgcat
    13381 gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa
    13441 gacggtcgtt actacgcgat agatttcacg ctggatgaaa ttaagtcgct gaaatttacc
    13501 gaaggtttcg atattgaaaa cggtaaaaaa gtacagactt atccggggcg tttcccaatg
    13561 ggtaagtccg acttccgggg gcacaccttt gaagaagaga ttgaatttgt tcaggggtta
    13621 aatcactcta ccgggaaaaa tatcggtatc tatccagaaa tcaaagcgcc gtggttccat
    13681 catcaggaag ggaaggatat tgcggcaaaa acgctggaag tgctgaagaa atatggttac
    13741 accggtaagg acgataaagt ttatttgcaa tgttttgatg ctgatgagct gaagcgtatt
    13801 aagaatgagc tggaacctaa aatgggcatg gatctcaatc tggtacagct gattgcctat
    13861 accgactgga atgaaacgca gcagaaacag ccggacggaa gctgggttaa ttacagctat
    13921 gactggatgt ttaagccagg tgccatgaag caagtcgcgg aatatgccga cggtatcggg
    13981 ccggattacc atatgttgat tgaggaaaca tcgcagccgg gtaatatcaa actcactggc
    14041 atggtgcaag atgctcagca gaataaactg gtagtgcatc cttataccgt gcggtcggat
    14101 aaactgcctg aatacactac tgatgtgaat cagttatatg atgctctgta taacaaagcg
    14161 ggtgtaaatg ggctgtttac tgatttccct gataaggcag taaaattcct taataaagag
    14221 taatgctact gcaggatggc gttcatgcgc catcctgtct catatcaaat ttttccaatc
    14281 gccactaatt gttccatgca catttgtagt aaaaacagtg gactgccttc taccggcttt
    14341 tctttagcca acaacgaaat ctgtcgatag aaggttggtt cgtgaggaac ggaacacaat
    14401 tcacctgcaa tggttttcag cgttaactct ggtaataagg cgatgcctaa tccctgtcga
    14461 ataaaattca tcgctgtatc cacctgatta aattcacatt ttatctgctg ggcgcactat
    14521 tagtggtcgt ggctggtgga atctatgcgc tgcaaatgaa gggcaaaagc ggtgtca
//
LOCUS       DAZOYS010000098        14442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOYS010000098 DAZOYS010000000
VERSION     DAZOYS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.098"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            71..427
                     /locus_tag="ACHUN9_002572"
     CDS             71..427
                     /locus_tag="ACHUN9_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0695933.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHUN9_002573"
                     /pseudo
     CDS             complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHUN9_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1475..2176
                     /locus_tag="ACHUN9_002574"
     CDS             1475..2176
                     /locus_tag="ACHUN9_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0695934.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHUN9_002575"
     CDS             2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHUN9_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0695935.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2497..3807)
                     /gene="cyuA"
                     /locus_tag="ACHUN9_002576"
     CDS             complement(2497..3807)
                     /gene="cyuA"
                     /locus_tag="ACHUN9_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0695936.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3835..5166)
                     /locus_tag="ACHUN9_002577"
     CDS             complement(3835..5166)
                     /locus_tag="ACHUN9_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0695937.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5441..6805)
                     /gene="tdcG"
                     /locus_tag="ACHUN9_002578"
     CDS             complement(5441..6805)
                     /gene="tdcG"
                     /locus_tag="ACHUN9_002578"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0695938.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6877..7266)
                     /locus_tag="ACHUN9_002579"
     CDS             complement(6877..7266)
                     /locus_tag="ACHUN9_002579"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0695939.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7280..9574)
                     /gene="tdcE"
                     /locus_tag="ACHUN9_002580"
     CDS             complement(7280..9574)
                     /gene="tdcE"
                     /locus_tag="ACHUN9_002580"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0695940.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9608..10816)
                     /gene="tdcD"
                     /locus_tag="ACHUN9_002581"
     CDS             complement(9608..10816)
                     /gene="tdcD"
                     /locus_tag="ACHUN9_002581"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0695941.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10842..12173)
                     /gene="tdcC"
                     /locus_tag="ACHUN9_002582"
     CDS             complement(10842..12173)
                     /gene="tdcC"
                     /locus_tag="ACHUN9_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0695942.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12195..13184)
                     /gene="tdcB"
                     /locus_tag="ACHUN9_002583"
     CDS             complement(12195..13184)
                     /gene="tdcB"
                     /locus_tag="ACHUN9_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0695943.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13283..14221)
                     /gene="tdcA"
                     /locus_tag="ACHUN9_002584"
     CDS             complement(13283..14221)
                     /gene="tdcA"
                     /locus_tag="ACHUN9_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0695944.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3749 a         3863 c         3361 g         3469 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttatccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtctgga gcttccatat ctgtctatgc tttatttgct
      241 ggcaaccttc cttcccgtcc ttgcgcttgc tatccgtcgc ttacacgata ccgaccgttc
      301 aggtgcctgg gccttgctct tttttgttcc gtttattggc tggctcgtgc ttctggtttt
      361 tttctgcaca gaaggtactt ctggcagcaa tcgttacgga aacgatccga agtttggttc
      421 aaattaatct tagaattggg gcgatatttc gccccttttt attaacaata ataaatatta
      481 ttttccgcta aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact
      541 gtcacgacgc caggccataa taatatcgat ctcactggtc gattccgggc tgacgacacg
      601 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
      661 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
      721 gtccagcagt tgtacggtca acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc
      781 acgatatttc acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
      841 aggcgcggca acgtagacgt tcattaacgt atagagcttg cgcgagttga tctccgacga
      901 ggaacgaaaa tgcatatccg gcgcgataac aatatccgcc cgcccctgct ccagtcgttc
      961 ctacgccccc gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag
     1021 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcttcggtca caatggtgag
     1081 atgcgtttcc caaccgcgcg cgagggcttc cgcatcggta gtcagtttat ctgcggcttc
     1141 cagcaaaacg cgccctcgct ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg
     1201 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
     1261 aagtgcggaa ggcacgcgtc ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc
     1321 gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca
     1381 ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct
     1441 taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca
     1501 gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta
     1561 cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc
     1621 cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct
     1681 ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga
     1741 ggcgttgctg ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga
     1801 caaaccgtta acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct
     1861 gattcaaaaa ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac
     1921 gatgggaagc ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg
     1981 cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa
     2041 atttcatgcg cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt
     2101 tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat
     2161 agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa
     2221 agcgccagcc agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg
     2281 gctatgaaga gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag
     2341 ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg
     2401 gcctacaaaa tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc
     2461 agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat
     2521 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2581 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2641 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     2701 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2761 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2821 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     2881 tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg
     2941 ttctgctaca accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt
     3001 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     3061 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     3121 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     3181 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     3241 gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc
     3301 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     3361 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     3421 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3481 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3541 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3601 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3661 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     3721 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     3781 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     3841 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     3901 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     3961 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     4021 tacgatagcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     4081 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     4141 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     4201 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     4261 cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag
     4321 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     4381 tgcccgcaat gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat
     4441 caccatcggg cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat
     4501 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     4561 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     4621 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     4681 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     4741 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     4801 cattacgaaa tagaacgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     4861 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     4921 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     4981 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     5041 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     5101 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     5161 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     5221 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     5281 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     5341 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     5401 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     5461 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     5521 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     5581 tccacgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     5641 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     5701 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     5761 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
     5821 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
     5881 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     5941 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     6001 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag
     6061 aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca
     6121 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     6181 atcctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag
     6241 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     6301 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     6361 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
     6421 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     6481 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
     6541 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     6601 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca
     6661 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     6721 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
     6781 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     6841 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
     6901 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
     6961 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     7021 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
     7081 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
     7141 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     7201 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     7261 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
     7321 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     7381 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     7441 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     7501 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
     7561 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
     7621 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
     7681 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
     7741 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
     7801 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     7861 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
     7921 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
     7981 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt
     8041 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
     8101 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
     8161 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
     8221 taattgcgta cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
     8281 taccaatcac cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag
     8341 tacgcatcag atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
     8401 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
     8461 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
     8521 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
     8581 acagcgaatc aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt
     8641 gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa
     8701 tgtagatatc gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt
     8761 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg
     8821 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
     8881 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca
     8941 gacgggactg gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat
     9001 acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac
     9061 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     9121 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac
     9181 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc
     9241 gctttaatgg cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc
     9301 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
     9361 ttgcgttttc gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg
     9421 tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
     9481 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     9541 acagcttatc gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc
     9601 aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag
     9661 caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg
     9721 aaacaattcg ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc
     9781 ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc
     9841 cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac
     9901 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
     9961 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
    10021 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
    10081 acatcgcacc aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg
    10141 tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
    10201 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg
    10261 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
    10321 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
    10381 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
    10441 taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    10501 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    10561 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
    10621 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    10681 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
    10741 cgctggcatc gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    10801 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    10861 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    10921 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat
    10981 gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca
    11041 acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac
    11101 actttagttt tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc
    11161 gttcccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    11221 gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    11281 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    11341 agcgtaaata ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    11401 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    11461 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc
    11521 atgatggaaa tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc
    11581 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    11641 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    11701 tccttaccaa accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg
    11761 cgattcagcg gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata
    11821 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    11881 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    11941 gagccagaaa gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    12001 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    12061 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    12121 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    12181 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    12241 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    12301 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    12361 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    12421 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    12481 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    12541 ccgtgaacgt tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    12601 gccaccgcaa taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca
    12661 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    12721 tcatcgtaag gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg
    12781 atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    12841 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    12901 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg
    12961 cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    13021 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    13081 tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    13141 ataatatcat caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc
    13201 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt
    13261 atgtttaaat aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat
    13321 tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat
    13381 aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag
    13441 taataaattg attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat
    13501 cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa
    13561 tactgatgcc atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt
    13621 gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc
    13681 gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct
    13741 gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg
    13801 ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct
    13861 ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa
    13921 atgagacttc caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca
    13981 tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta
    14041 caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga
    14101 ttttactgac ggccggttga gttaacccta attcttttgc agccgagccg atagagccac
    14161 ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca
    14221 taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag
    14281 gatatgtgcg accactcaca aattaacttt caatactttc cagggtatcg tcattacaat
    14341 taaatacctt taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg
    14401 tcaaaaaaaa tgacggggat aaccatattt tatggcgata ac
//
LOCUS       DAZOYS010000099        14424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOYS010000099 DAZOYS010000000
VERSION     DAZOYS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.099"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUN9_002585"
     CDS             <1..161
                     /locus_tag="ACHUN9_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0695945.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHUN9_002586"
     CDS             complement(199..1260)
                     /locus_tag="ACHUN9_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0695946.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUN9_002587"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUN9_002587"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0695947.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLLERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUN9_002588"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUN9_002588"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0695948.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHUN9_002589"
     CDS             complement(3454..4155)
                     /locus_tag="ACHUN9_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0695949.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUN9_002590"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUN9_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0695950.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUN9_002591"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUN9_002591"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0695951.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHUN9_002592"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHUN9_002592"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0695952.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHUN9_002593"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHUN9_002593"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0695953.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHUN9_002594"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHUN9_002594"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0695954.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHUN9_002595"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHUN9_002595"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0695955.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHUN9_002596"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHUN9_002596"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0695956.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3802 a         3692 c         3602 g         3328 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgctggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taag
//
LOCUS       DAZOYS010000100        14001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOYS010000100 DAZOYS010000000
VERSION     DAZOYS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.100"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUN9_002597"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUN9_002597"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0695957.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUN9_002598"
     CDS             2273..2662
                     /locus_tag="ACHUN9_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0695958.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUN9_002599"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUN9_002599"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0695959.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUN9_002600"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUN9_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0695960.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUN9_002601"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUN9_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0695961.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUN9_002602"
     CDS             4687..5232
                     /locus_tag="ACHUN9_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0695962.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUN9_002603"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUN9_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUN9_002604"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUN9_002604"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0695963.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUN9_002605"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUN9_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0695964.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUN9_002606"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUN9_002606"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0695965.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUN9_002607"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUN9_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0695966.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUN9_002608"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUN9_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0695967.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUN9_002609"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUN9_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0695968.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUN9_002610"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUN9_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0695969.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUN9_002611"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUN9_002611"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0695970.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUN9_002612"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUN9_002612"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0695971.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3401 a         3545 c         3717 g         3338 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa t
//
LOCUS       DAZOYS010000101        13868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOYS010000101 DAZOYS010000000
VERSION     DAZOYS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.101"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            22..1788
                     /gene="adeD"
                     /locus_tag="ACHUN9_002613"
     CDS             22..1788
                     /gene="adeD"
                     /locus_tag="ACHUN9_002613"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0695972.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(1833..3224)
                     /gene="uhpT"
                     /locus_tag="ACHUN9_002614"
     CDS             complement(1833..3224)
                     /gene="uhpT"
                     /locus_tag="ACHUN9_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0695973.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3362..4681)
                     /gene="uhpC"
                     /locus_tag="ACHUN9_002615"
     CDS             complement(3362..4681)
                     /gene="uhpC"
                     /locus_tag="ACHUN9_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0695974.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4691..6193)
                     /gene="uhpB"
                     /locus_tag="ACHUN9_002616"
     CDS             complement(4691..6193)
                     /gene="uhpB"
                     /locus_tag="ACHUN9_002616"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0695975.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6193..6783)
                     /gene="uhpA"
                     /locus_tag="ACHUN9_002617"
     CDS             complement(6193..6783)
                     /gene="uhpA"
                     /locus_tag="ACHUN9_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0695976.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6858..7148)
                     /gene="ilvN"
                     /locus_tag="ACHUN9_002618"
     CDS             complement(6858..7148)
                     /gene="ilvN"
                     /locus_tag="ACHUN9_002618"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0695977.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7152..8840)
                     /gene="ilvB"
                     /locus_tag="ACHUN9_002619"
     CDS             complement(7152..8840)
                     /gene="ilvB"
                     /locus_tag="ACHUN9_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0695978.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8946..9044)
                     /gene="ivbL"
                     /locus_tag="ACHUN9_002620"
     CDS             complement(8946..9044)
                     /gene="ivbL"
                     /locus_tag="ACHUN9_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0695979.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9609..9698
                     /gene="tisB"
                     /locus_tag="ACHUN9_002621"
     CDS             9609..9698
                     /gene="tisB"
                     /locus_tag="ACHUN9_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0695980.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9822..9896)
                     /gene="ysdE"
                     /locus_tag="ACHUN9_002622"
     CDS             complement(9822..9896)
                     /gene="ysdE"
                     /locus_tag="ACHUN9_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0695981.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9978..11162
                     /gene="emrD"
                     /locus_tag="ACHUN9_002623"
     CDS             9978..11162
                     /gene="emrD"
                     /locus_tag="ACHUN9_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0695982.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11170..11667)
                     /locus_tag="ACHUN9_002624"
     CDS             complement(11170..11667)
                     /locus_tag="ACHUN9_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0695983.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQEMPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11664..12026)
                     /locus_tag="ACHUN9_002625"
     CDS             complement(11664..12026)
                     /locus_tag="ACHUN9_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0695984.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12016..12363)
                     /locus_tag="ACHUN9_002626"
     CDS             complement(12016..12363)
                     /locus_tag="ACHUN9_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0695985.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12472..12921
                     /gene="yidI"
                     /locus_tag="ACHUN9_002627"
     CDS             12472..12921
                     /gene="yidI"
                     /locus_tag="ACHUN9_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0695986.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12968..13126)
                     /locus_tag="ACHUN9_002628"
                     /pseudo
     CDS             complement(12968..13126)
                     /locus_tag="ACHUN9_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13170..>13868)
                     /locus_tag="ACHUN9_002629"
     CDS             complement(13170..>13868)
                     /locus_tag="ACHUN9_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0695987.1"
                     /translation="TPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARL
                     QRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVS
                     NMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYT
                     SQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         3259 a         3856 c         3624 g         3129 t
ORIGIN      
        1 taaaatcaaa atgagaaaaa tatgaataat tctattaacc ataaatttca tcacattagc
       61 cgggctgaat accaggaatt gttagccgtt tcccgtggcg acgctgttgc cgattatatt
      121 attgataatg tctctattct cgacctgatc aatggcggag aaatttccgg cccaattgtg
      181 attaaaggac gttacattgc cggtgttggc gcagaatacg ctgatgctcc ggctttgcag
      241 cggattgatg ctcgcggcgc aacggcggtg ccagggttta ttgatgctca cctgcatatt
      301 gaatccagca tgatgacgcc ggtcactttt gaaaccgcta ccctgccgcg cggcctgacg
      361 accgttattt gcgaccctca tgaaatcgtc aacgtgatgg gagaagccgg attcgcctgg
      421 tttgcccgct gtgccgaaca ggcaaggcaa aaccagtact tacaggtcag ctcttgcgta
      481 cccgccctgg aaggctgcga tgttaacggt gccagtttta cccttgaaca gatgctcgcc
      541 tggcgggacc atccgcaggt taccggcctt gcagaaatga tggactaccc tggcgtaatt
      601 agcgggcaga atgcgctgct cgataaactg gatgcatttc gccacctgac gctggacggt
      661 cactgcccgg gtttgggtgg taaagaactt aacgcctata ttactgcggg tattgaaaac
      721 tgccacgaaa gttatcagct ggaagaagga cgccggaaat tacaactcgg catgtcgttg
      781 atgatccgcg aagggtccgc tgcccgcaat ctcaacgcgc tggcaccgtt gatcaacgaa
      841 tttaacagcc cgcaatgcat gctctgtacc gatgaccgta acccgtggga gatcgcccat
      901 gaaggacaca tcgatgcctt aattcgccgc ctgatcgaac aacacaatgt gccgctgcat
      961 gtggcatatc gcgtcgccag ctggtcgacg gcgcgccact ttggtctgaa tcacctcggc
     1021 ttactggcac ccggcaagca ggccgatatc gtcctgttga gcgatgcgcg taaggtcacg
     1081 gtgcagcagg tactggtgaa aggcgagccg attgatgcgc aaaccttaca ggcggaagag
     1141 tcggcgagac tggcacaatc cgctccgcca tatggcaaca ccattgcccg ccagccagtt
     1201 tccgccagcg actttgccct gcaatttacg cccggaaaac gctatcgggt cattgacgtc
     1261 atccataacg aattgattac gcactcccac tccagcgtct acagcgaaaa tggttttgat
     1321 cgcgatgatg tgtgctttat tgccgtactt gagcgttacg ggcaacggct ggctccggct
     1381 tgtggtttgc ttggcggctt tggactgaat gaaggtgcgc tggctgcgac ggtcagccat
     1441 gacagccata atattgtggt gatcggtcgc agtgccgaag agatggcgct ggcggtcaat
     1501 caggtgattc aggatggcgg cgggctgtgc gtggtacgta acggccaggt ccaaagtcat
     1561 ctgccgttgc ccattgccgg cctgatgagc accgacacgg cgcagtcgct ggcggaacaa
     1621 attgacgcct tgaaagccgc cgcccgtgaa tgcggtccgt tacccgatga gccgtttatt
     1681 cagatggcgt ttctttctct gccagtgatc cccgcgctaa aactaaccag tcaggggcta
     1741 tttgatgtcg agaagtttgc cttcactacg ctggaagtca cggaataata aaaaagcccg
     1801 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     1861 ttctcgcggc ggattttgcg ttcttccatc accgccacta tcgccatcag gcagatacaa
     1921 ccaatcgcgg cgatatcaag tgcggcgaag gtgcctgccc agccggtaag gccgaatacc
     1981 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     2041 gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     2101 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     2161 gccagataga tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc
     2221 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ctaggtcaga gagccagccc
     2281 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     2341 gctttagaga gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata
     2401 cgtaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     2461 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     2521 tcgctgatct cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag
     2581 tcgctgccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata
     2641 aacatgccga tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacgcctgct
     2701 gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta
     2761 cgacgcggcg tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa
     2821 aagccgctta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     2881 ctggcactga agcccagcat acaaatggca gacaggatca gcatgaacgg caggaattgt
     2941 ttggtgtttt tgccgtcggc gtagtaggaa accagcgttt tacccacgcc ataagtgatg
     3001 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgagatcatg
     3061 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     3121 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     3181 aggtccaggg tcggcttgcg aacctggttt aagaaagcca gcatgggtta ctcctgaaat
     3241 gaatacctgc gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt
     3301 ccaggcaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc
     3361 atcacgcttc gcgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc
     3421 cggcggcgat agagataacc acaaaaaatc cgctccagtg ccaggtatcg agtactttcg
     3481 ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg
     3541 ccgcccctgc cgcctctttg tgggaacact ctgccgccgc cataccgatt aacatctgtg
     3601 ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa
     3661 atggcatcag ccacagggag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca
     3721 tcggccctcg gttgccgtta aacaatttgt ccgagcccca acccgctacc agcgcaccga
     3781 taaatccacc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca
     3841 gcgtctcgga catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca
     3901 gcacatagca aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga
     3961 tctctttacg cgtcagccct gccccttctt gttgttgagc tatctccagc gcgtcgtgtc
     4021 gccattcacc gaccgccggt aaacctaacg cctgcgggcg atcgcgcagc cgccagcaga
     4081 gaaaaattcc cacgacgatc gccatacaac cagcaatcat catcccggca cgccagccgt
     4141 aatgtagtgc agccgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg
     4201 tgttccataa tgcccaccaa ccaccgcgct cggtacgtga ataccaggcc gttaacagac
     4261 gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agtacggcaa
     4321 acgcccatag cgacgtcgag aagccaaaca gaatgttgat aatgcccgtg gcgataagcc
     4381 ctatccccat aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg
     4441 acacgccata ggtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca
     4501 cgccgttagc aaggatttct ggtacggcgg cgttaaaact tttccgcgtg aagtaaaaca
     4561 gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga tagcgggcat
     4621 caatttcata tttatcagcc attaatggcg catcggcagg cgctttcaga aacggcaaca
     4681 tcatcaaacc ttagacatag cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac
     4741 aggaaatgtg taatgtgcca cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa
     4801 aaccttgttg cccggaaccc ggcggcaaac cgctgccatc gtcttcaata accagcatca
     4861 accgttcatc ctgctgccag ccttgcaggg tgaccgcgct ggcatcagca tgtttcacaa
     4921 tgttgttcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaacg
     4981 ctgattcatc gattcgccat tcgagatggc tgacaatacc gcgcccttcc agctccattt
     5041 cccgcatcag tgagcggatg gcctgctcca gggtgagatc atccaactgg cgcggacgta
     5101 accgacccaa caaacggcgc accgcgtcgt aaacgcccag cgatagttgt tcgatgagtt
     5161 gcccgctctg cttcacgctg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag
     5221 tacgaatagc agtgatggtc tggccgatat catcatgcag ctcacgcgcc acatcacggc
     5281 gcacgctctc ttcggtttcc agcaaccgtt cagccagatg ctgattgcgc gccagttcct
     5341 tttgcagcga ctggttaagt tcacgcaacc gctggatgcc agcgccaagc aacaaccctg
     5401 tcagactttg caccagcagc gagagcaata aatccaccgg atgatcgcgc caggtttgac
     5461 tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag cgccccttgc caaccatagt
     5521 gccaggccag cgcgataatc ggcagcacca gacagaatgg cgtaaagcgc gacagttcgt
     5581 ccggcaatcc caactggagc cagagactga taacaaacag cagcaagtac cagaccagat
     5641 gtcgcccgcg ccagttgatt ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt
     5701 tattggcgag atagtgccag aacaccagac atatcggggc cagcgtcagg ccgccagtta
     5761 aagtcagcaa cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca
     5821 acaacgccgc cgccgttaac gccgccccct gtaacagcaa ggtgcgccag tcacgctgat
     5881 ggcgatagcg cgagatcagc gctacgggca gtaacgtcag taaactaccg atcatcaata
     5941 acggaaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac
     6001 ccagcaatac gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa
     6061 acggaaataa cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccaca
     6121 ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga
     6181 acaacgtctt catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac
     6241 gcccagtttt tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag
     6301 ttcggcggca atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc
     6361 acgtttggtt agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag
     6421 ataacagcca cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg
     6481 tttggaaaga aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc
     6541 gtgaacggag agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc
     6601 ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc
     6661 cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc
     6721 cagcagctgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat
     6781 catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt
     6841 gttcaagcct tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg
     6901 gactgattac gctgcacttt cacgacatct tccagcttat cgatttggct tatcatctgc
     6961 tccagacgct ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc
     7021 ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg
     7081 gtcattacgc ccggatggtt gcgaacggtg agctccagaa ttacgttgtc atgagttgtg
     7141 ttttgcatgg cttattcctc caccatttca gtattcgccg cacctggcgg caccatcggg
     7201 taaacttttt cttcggcatc aatgcgcaca tggatcagcg ccgggctagg gcgattgatg
     7261 atttcctgca atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg
     7321 aatccggcgg caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct
     7381 tgctcgtaga acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga
     7441 atgattttga catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc
     7501 aggctgccgt cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca
     7561 atcgccgcag gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt
     7621 gggcgattga gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg
     7681 ataatcgcat tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat
     7741 ggatcgcacg ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg
     7801 tgccactctg cacgcggttg cgcttccacc tgcgggatca attgcgccag cacgtcatca
     7861 acatccgcct gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata
     7921 tcgacatgaa taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca
     7981 tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg
     8041 cgcacgccgt gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc
     8101 agcgccatta aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc
     8161 gcaggcgcat tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc
     8221 attgtagcta cgtcacgaat gctttcttcg ctaaaggcgg gtgcagcggc tttttctgcc
     8281 accgcgggct gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc
     8341 cacaccgggc cagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgcggg
     8401 agttcttcga tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag
     8461 gtgtcgactt cctggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg
     8521 caaatcagcg ggatggagtc cagccgcgca tcggcaatgg cggtcaccag gttagtcgca
     8581 cccggtccgc tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga
     8641 gcgataaagc ccgcgccctg ttcatgacgg gccagaatat ggcggatttg cgtgctttgg
     8701 cttaaggcat cgtaaacagg caggatagaa ccgcccggaa tgcctgtcac aatcttaatg
     8761 ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcaat
     8821 gttgtgcccg aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg
     8881 cttaaacgaa aaaccccgcc cggtttgcgc cgacggggtt ttggaatcgt gtgttgttcc
     8941 agtccctacg gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat
     9001 ggcgcagttg gtagtagttt tgcgttgaac atggaagtag tcattgggga ccttgttggt
     9061 tttgtgttta acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt
     9121 cagcaaggga aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac
     9181 cccgccggag cgaggtttcg tcagtcgccc gcggctggta accgcaaagc acactgtatt
     9241 atgtcaacac agaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg
     9301 acgtatttag tgcatagttg agtatcgatc acagtttgcg ttttgtccaa atattactgt
     9361 ttatttatac agtaaacttc tataatatca ctgtacgcaa tgtgttatgc gggggccgca
     9421 tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc
     9481 gcgtgagcac actgtgttat gtcaacaagc acaaagtttc tccttgagat accgcgtgca
     9541 caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg
     9601 agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac
     9661 tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa
     9721 ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggtgtc ggatgcgatg
     9781 ctgacgtatc ttatccgccc taccatctct cccggcaaca tttattgccg cttttgttta
     9841 catattctgc cgctaaacaa ctccccattc ctggcgtata tctggctaac attcatcaat
     9901 gtgatagatt cctctcccgc atttatggga atgcgtagtg acttactcta attattttta
     9961 taaaagcatc cgtgataatg aaaaggcata gaaacgtcaa tttgttattg atgttggtat
    10021 tactcgtggc cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg
    10081 cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga
    10141 cttacggtgt ctcacagctg ttttatggcc cgatttccga ccgtgtgggt cgccgaccgg
    10201 tgatcctcgt cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca
    10261 gtttgacggt attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg
    10321 taatggcgcg tactttgccg cgagatttat atgaacggac acagttgcgc catgctaaca
    10381 gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc
    10441 tggatacgat gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg
    10501 tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc
    10561 gcctgcttac cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga
    10621 tgctgattgg cggtctggcc gggattgccg cctttgaagc ctgctccggc gtgctgatgg
    10681 gcgcggtgtt agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg
    10741 cagcgttttt tggcgcatgg tttgccggac gtcccaataa acgcttctcc acgttaatgt
    10801 ggcagtcggt tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg
    10861 gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga
    10921 tgctgtttcc tctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg
    10981 ctggcgcgct ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg
    11041 cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt
    11101 tgatcgtgct gtgctggcta ccgctggcga cgcggatgtc gcatcagggg cagcccgttt
    11161 aagcgcacgt caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag
    11221 atcacgcatc acccgcatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca
    11281 aagcacgctg tctggcatct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc
    11341 ctggcagtca cagcgcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc
    11401 aaaaagctca atcgagatac gactgatatc tgcccgcacc caacactcga acagggcaat
    11461 taaaaatttc ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc
    11521 aaattcaaga tagggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata
    11581 aaaatcggca ggttgcagag catccttgta ctctacaacc agtttatgcc tgtcctcttc
    11641 agcatcaatg acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt
    11701 atatgcgtta cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg
    11761 tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta
    11821 cgggtgtagt gccagaggat cagcgccacg atggcgagaa tgccaatcga aatccagaat
    11881 aacatgcccg cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc
    11941 agcatggtac gaaaccacgc cagtgatgtg cgctccggct gcagtcctgg gtcggcaatg
    12001 cgtctggctt tgcggctatc cggcatacaa caccagtccc ataacgatca ccgccacgac
    12061 catcaatatt aagctaatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg
    12121 catcgctttt tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa
    12181 cagacacaac aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag
    12241 ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa
    12301 agtacgctca ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt
    12361 cattgttgct cctttgcaat ttcactaact ctctaccttt agcggttttt tttaagaatt
    12421 tcgtccgccc gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc
    12481 atagcgcaaa ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg
    12541 gggattatcc acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc
    12601 gtggcggaca aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa
    12661 attaccaccg tggagaaaaa agaggacgta gatgtcgata aaatcctcga tcaaagcggt
    12721 atcgcactgg ccattgctgc gctactttgc gcatttgttg gcggtatgcg taaggaaaat
    12781 cgctggggca ttcgcggcgc gctggttttt ggcggcggaa cgctggcttt ccataccttg
    12841 ctttttggta tcggcatcgt gtgcagcatt ttgctgatat tccttatctt ttccttcctg
    12901 accggcggtt cgctggttta agcagcctga ataatgcccg ccgggtgatc atcagcgggc
    12961 attatcgtca gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaggccgg
    13021 tgtcatagtc acgtaccacc ggcgaatagc catcttgtgg gcgtggacga aatgccccca
    13081 tccagcgcgg ccgtgcatct ttacgccacg gacgcaggct ccattgttga catcctccac
    13141 gccctgaagg acgtggaccc ctgctacgtt caggctgtcg cctgaatcat ttcggtgggt
    13201 tcctgcttca acgagcggcc tgactgcacc atctctccac aggcaagaac ggcgtgcccc
    13261 gccgctaaaa tgttacgagc gccatttaca tcggcgttcg ctgtatatcc acatacctgg
    13321 catctgaatt tactttgtga caggcgattt tctttcgctg tatgaccaca gcacacgcaa
    13381 cgctggcttg tgtacgctgg cggaacagca agcacctgac caccacgcca gagctgcttg
    13441 tacgcaagct ggcggcgcat ttcataccag ccctgatcca gtatcgaacg gtttaaacct
    13501 gattttgccc ggacattgcg ccccggcagg ctgaccgtac ccgctgctga ctttgacatg
    13561 ttgctgacct tcaaatcctc aatgactatc attgcgtggt ttttgctgac ggtcgttgtg
    13621 actttgtgaa ggtagtccct gcggatattt gcgatacagg aatgcagtcg ctgtattttg
    13681 cgtttctgct tctgccagtt gttgctgaat ttgaccttgc ggcttaactg tcgctgaagt
    13741 ctcgccagcg tcttctggtt tttctgaaaa ctgtttacag gctcaaagac tgtgccatct
    13801 gacagcgtgg cgagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga
    13861 gccggagt
//
LOCUS       DAZOYS010000102        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOYS010000102 DAZOYS010000000
VERSION     DAZOYS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.102"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUN9_002630"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUN9_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUN9_002631"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUN9_002631"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0695988.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUN9_002632"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUN9_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0695989.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUN9_002633"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUN9_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0695990.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHUN9_002634"
     CDS             4177..5415
                     /locus_tag="ACHUN9_002634"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR0695991.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUN9_002635"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUN9_002635"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0695992.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUN9_002636"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUN9_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0695993.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUN9_002637"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUN9_002637"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0695994.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUN9_002638"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUN9_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0695995.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUN9_002639"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUN9_002639"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0695996.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNFASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUN9_002640"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUN9_002640"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0695997.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUN9_002641"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUN9_002641"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR0695998.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUN9_002642"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUN9_002642"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0695999.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUN9_002643"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUN9_002643"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0696000.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUN9_002644"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUN9_002644"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0696001.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3506 c         3688 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaaagtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZOYS010000103        13849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOYS010000103 DAZOYS010000000
VERSION     DAZOYS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.103"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..260)
                     /locus_tag="ACHUN9_002645"
     CDS             complement(<1..260)
                     /locus_tag="ACHUN9_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0696002.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            367..663
                     /locus_tag="ACHUN9_002646"
     CDS             367..663
                     /locus_tag="ACHUN9_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0696003.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(797..1135)
                     /locus_tag="ACHUN9_002647"
                     /pseudo
     CDS             complement(797..1135)
                     /locus_tag="ACHUN9_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(1160..2449)
                     /gene="arsB"
                     /locus_tag="ACHUN9_002648"
     CDS             complement(1160..2449)
                     /gene="arsB"
                     /locus_tag="ACHUN9_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0696004.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2503..2856)
                     /gene="arsR"
                     /locus_tag="ACHUN9_002649"
     CDS             complement(2503..2856)
                     /gene="arsR"
                     /locus_tag="ACHUN9_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0696005.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3395..3568)
                     /locus_tag="ACHUN9_002650"
     CDS             complement(3395..3568)
                     /locus_tag="ACHUN9_002650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696006.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3596..3679
                     /gene="dinQ"
                     /locus_tag="ACHUN9_002651"
     CDS             3596..3679
                     /gene="dinQ"
                     /locus_tag="ACHUN9_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0696007.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3733..5085)
                     /gene="gorA"
                     /locus_tag="ACHUN9_002652"
     CDS             complement(3733..5085)
                     /gene="gorA"
                     /locus_tag="ACHUN9_002652"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0696008.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5157..5999)
                     /gene="rlmJ"
                     /locus_tag="ACHUN9_002653"
     CDS             complement(5157..5999)
                     /gene="rlmJ"
                     /locus_tag="ACHUN9_002653"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0696009.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6202..8244
                     /gene="prlC"
                     /locus_tag="ACHUN9_002654"
     CDS             6202..8244
                     /gene="prlC"
                     /locus_tag="ACHUN9_002654"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0696010.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8252..9004
                     /gene="rsmJ"
                     /locus_tag="ACHUN9_002655"
     CDS             8252..9004
                     /gene="rsmJ"
                     /locus_tag="ACHUN9_002655"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0696011.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9053..10522)
                     /gene="dtpB"
                     /locus_tag="ACHUN9_002656"
     CDS             complement(9053..10522)
                     /gene="dtpB"
                     /locus_tag="ACHUN9_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0696012.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10688..10741
                     /locus_tag="ACHUN9_002657"
     CDS             10688..10741
                     /locus_tag="ACHUN9_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696013.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10840..11274)
                     /gene="uspA"
                     /locus_tag="ACHUN9_002658"
     CDS             complement(10840..11274)
                     /gene="uspA"
                     /locus_tag="ACHUN9_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0696014.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11665..12000
                     /gene="uspB"
                     /locus_tag="ACHUN9_002659"
     CDS             11665..12000
                     /gene="uspB"
                     /locus_tag="ACHUN9_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0696015.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12244..13743)
                     /gene="pitA"
                     /locus_tag="ACHUN9_002660"
     CDS             complement(12244..13743)
                     /gene="pitA"
                     /locus_tag="ACHUN9_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0696016.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
BASE COUNT         3493 a         3585 c         3631 g         3140 t
ORIGIN      
        1 ccggctttcg ctgacaacaa tccacgtaac atgggtattg tcgagcaact acagaacggg
       61 gtaacggctc ccaacagagc agccaggaga tagcctctcc cttttctggc ccccatcatc
      121 tgctggattt tatggtctgg caccttttgt cttatcaggc tgacaccggc actaatcact
      181 ataaacagca acgaaagctc gacggcgaga aacacaaaca tctctgcggc atcttgcagc
      241 atcgcaagcc aactactcat acgtaacctc attggataaa tctatatttc cagaataatc
      301 gaaatgaaat tcagatcaag atatttctgg tattatcgaa atatcaacca gacagagggg
      361 tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag agctaggtca cccgacccgt
      421 ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag ggttgccggt cggtgagttg
      481 caaaagcagc ttgggatacc tggctccaca ctcagccacc atatatcagc actggtatcg
      541 gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga tgtgtgtttc gcaatacgca
      601 atgctggaag cgataattga attcttacgc gaggagtgct gcgtaaatag caaaattgcc
      661 tgatgcgcta cgctctacat atttcatgcc atatattgaa tttgcacggt tttgtaggtc
      721 ggataagacg cgttagcgtc gcatccgaca taaacaaagc gcactttacc aactatcggc
      781 agtggatatc gccgctttat ttcaggcgct tacccgcttc atcaaccact ttctcgccat
      841 cttccttgga gaatgcgcct ttttgcgcat ctggcagaat ttccagcacc acttctgaag
      901 ggcggcacag gcgagttccc agcggcgtca ccacaatcgg gcgattaatc agaatcgggt
      961 gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt atcttccgca aggcccagct
     1021 cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac ggaaatcccc atatcggcaa
     1081 tgagtttgac cagttcatcg cgcgttggcg gagtttccag ataatggata atggtaatgt
     1141 tgctcatatc agtatctcat tacaaagtga aagagagacg tagcgccagc gcagccagcg
     1201 tcacaaacag cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga
     1261 tagtcatatt cttctgcgaa agtacgtgca gccagagtag cgtagccagg ctaccaattg
     1321 gggtaatttt cggtcccaaa tcgcagccaa tcacattggc ataaaccatc gcttctttga
     1381 taacgccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat
     1441 tgttcataat agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca
     1501 ggccgttatc cgccagcacg ttgagtacgc cagaaagata ttccgttaat ccggcattgc
     1561 gcaggccata aaccaccaga tacatgccga gcgagaagat gacaatctgc cagggggcac
     1621 cgcgcaggac tttacccgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca
     1681 gcgcgcccac agctgcaatg gcgctcaccg gaatgccgag cggttccagg acgaaaaatc
     1741 ccaccagcag aagcagtaaa acaacccagc cagttttgaa cgtagcagga tctttgatcg
     1801 cttctgcggg agatttcagc agcgccatat cgtagttctg cggaatatct ttgcgaaaat
     1861 agagatgtag catcaccagc gtggcaacaa tcgcggcgat atccaccggc accatcaccg
     1921 aggcgtattc gcgaaagccg aggccaaaga aatcagcgga aacgatattc accaggttgg
     1981 agacaataag cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca
     2041 gcgtcgtgcc tttactgaat cctaaagcca gcagcatggc gatgacaatc ggcgtcaaaa
     2101 taagcgccgc gccatcattg gcaaacaggg cggcaacggc agcaccgagc aggacaatcc
     2161 aggtaaacag caagcgacca cgaccattac cccagcgtga gacgtgcagc gccgcccatt
     2221 caaaaaagcc ggactcatcc agcagcaggc tgatgataat gacggcgata aacgcagccg
     2281 tcgcgttcca gacgatattc cacaccaccg gaatatcacc cggatgaacc acgcccgtaa
     2341 ctaacgccag tactgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc
     2401 agataaccaa tacgatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcccg
     2461 gataaaacac atctgaaaat tcatatgtgt ttagctaaat ttttaactgc aaatgttctt
     2521 actgtccccg gaacagtttt gtcgagccag gttgcggaca atcgcctgaa ccttttcctg
     2581 ttcacatcgc caggcctcat caataatttt cgccgcccat gctggaatat gcggtgataa
     2641 gcggtaatga acccacttac cttgcttgcg gtccagcaat agcccgcttt cacgaagcaa
     2701 tgccaggtgg cgggagatct tgggctgcga ctgatcgagg gcagtacaaa gatcgcagac
     2761 gcataactct cccagctcgc tgagcagtaa aacgatgccc agacgggttt catcagcaag
     2821 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgtggcaggt
     2881 agtgtctctc ttcgaagcgg ataagtcaaa aacatatatg acttaacgaa tgtgtaagtg
     2941 cagaggaagg taataggtgt gaattttgag ttggctattc atttgaaagg cggtttgaat
     3001 caggacgtaa aaaaaccgac tttgcgtcgg ttttttactc ttcagcctga gttggtggct
     3061 ctggctggag tgagaatgcc cacatctaac ggttcaatac tattagccag tggctaacat
     3121 tgcaagaatt agcgatttct tcagctggcg gagggggcac ttcggtatat atcgcaatga
     3181 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     3241 aaaaaccgac tttgcgtcgg tttttttact cttcagccct gagttggtgg ctctggctgg
     3301 agtgagatag tcatcatcta acgcatcaat gctaatacca tactgaaact attgcaagga
     3361 catgctggtt ttataacctg aatgtactgt atgattatcc agttagctct gaggcatttt
     3421 cactctggta atgcgcataa acgctttcaa agtcctggtc agaagtacgg gtggtgccgt
     3481 taactgatgc tctggccgga gtgagatagt cgtcatctaa caatgagaca tgcgccgtga
     3541 caggcagtgg atgagtaagc ggatgcattc tcactccatc gcatggagaa aacgggtgat
     3601 tgataaagca atcatcgttc ttggagcgtt aattgcgctg ctggaactga tccgctttct
     3661 gcttcagctt ctgaactgat agcggaaacg taattaaggg ctaagagcac actactctta
     3721 gccctttaac atttagcgca tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac
     3781 ggtattgtcg aagtcttttt tggttgcgcc catcttcagc gccaccgcga agccctgcaa
     3841 catttcgtcc ataccaaagc caatgccgtg aataccgaca atcttctctt ctggcccaac
     3901 gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat acatcgcggt
     3961 gaaagaggat ttatacactt tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc
     4021 cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta
     4081 atccagatgc tcatccggct tgttattaaa caggcgttca gagagacggc gacccgctgc
     4141 aactgctacc ggtgtcagtt ccactgcacc cgtgttatcg cccaccgcgt aaataccttc
     4201 aatattggtg ttttgatatt tatcgacgac gatatagcct ttttcgttag ttttaacgcc
     4261 agcggcttct aggttgatgt tgtcattggc aggctcgcga ccaatcgccc aaatcaggca
     4321 atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt
     4381 cactaccgct ttcgggatgg cgttggtgtg cagctgcggg ccttcggcgt tcatcacttc
     4441 gaccagcgtt tcggaaatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa
     4501 cagatgcgtt ttcgcgccga ggccgttaat cacgcccgcc agctcaacgg cgatgtaacc
     4561 cgcgccaaca accgccacgc gctctggcaa agcaggaagg gcgaagaagc catcagaatc
     4621 aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgcctg tggcgatcag
     4681 aatatgatcg gccgtgatgg tttcgccgtt tacctccagc gttttggcat caacgaagcg
     4741 ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat aggaagtatg
     4801 aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga acttgttgat
     4861 ggtggtatcg aaaccataat cagggccgta catatggatc gcttcacgga tttgcgccgc
     4921 gtgccacatc acttttttcg gcacgcagcc gacgttcacg caggtgccgc ccagctcttt
     4981 ggcttcaatc agtgcgcatt tctggccgta catagccgcg cggttgatgg aggcgatacc
     5041 gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacag agtgtcctta
     5101 tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac
     5161 tccggcacga tccagcttac ggtggcgtgc ccggtgcctg ccggaaccag tttgctgtgc
     5221 agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt aatcacaatc
     5281 atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag ttcaatttgc
     5341 agaattttgc gaataccggt cgcttccaga tcgtggatca tgcgcttaat ttgctgacgc
     5401 agcaccaccg gataccacag tgcgtaagta ccagtggcga aacgtttgta accttctgct
     5461 atcccgctga ccaccgcctg atagtcggtt ttcatttcat acggcgggtc gataaggatt
     5521 aaaccacggc gggaaaccgg cggaagtttg gccttaagct gctggaaacc gtcggctttt
     5581 tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg
     5641 tgcagttcgg tcagttgcag gctgtcttgt tcgcgcagta gctggcgagc aatcaacggc
     5701 gacccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac attgatgtac
     5761 gcctccagtt ctgcgggcaa atcgtcctgc tgccagatac gggcgatacc ttcgagatat
     5821 tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggta
     5881 tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag gctctgaacg
     5941 gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg
     6001 ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcgtatca
     6061 ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca
     6121 ctacacttaa ccccatgcta cacacattat gtaaagcgcc tgttgagcgc ttccttaacc
     6181 tctttaacca ggactgcgcg aatgacgaat ccgttactga ctccctttga attgcctccg
     6241 ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctcaggcgct gaacgattgc
     6301 cgcgaaaatg tggcgcgcgt agtagcgcaa ggggcaccgt acacctggga aaatctctgc
     6361 cagccgttgg cggaagtgga cgatgtgttg gggcgtatct tttccccggt cagccacctg
     6421 aactcggtga aaaatagccc ggaactgcgt gaagcctacg agcaaaccct gccgctgctg
     6481 tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta ccgcgacctg
     6541 cgcgatggcg atcattacgc cacgctgaac acggcgcaga agaaagcggt tgataacgcc
     6601 ctgcgtgatt ttgaactctc tggcataggt ctgccgaaag agaaacagca gcgttacggc
     6661 gaaattgcta cccgtctttc tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg
     6721 acgatgggct ggaccaaact cgttaccgac gaagcggggc tggcggggat gccagaaagc
     6781 gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg
     6841 ctggatatcc caagctacct gccggtaatg acctactgcg acaaccaggc tctgcgcgaa
     6901 gagatgtatc gcgcttacag cacccgcgcc tccgatcaag gcccgaacgc cggtaagtgg
     6961 gataacagca aggtgatgga agagatcctc gcgctgcgtc acgaactggc gcaactgctg
     7021 ggctttgaaa actacgcctt taaatccctt gccactaaaa tggcagaaaa cccgcagcag
     7081 gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg
     7141 gagcaactgc gtgccttcgc taaagccgaa tttggtgtcg atgagttgca gccgtgggat
     7201 atcgcttact acagcgaaaa acagaaacag cacctctaca gcatcagcga cgagcagctg
     7261 cgtccgtact tcccggaaaa caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt
     7321 tacggcatca ccgctaaaga gcgtaaagag gttgatgtct ggcatccgga tgtacgtttc
     7381 ttcgaactgt atgacgaaaa taacgaactg cgcggcagct tctacctcga tctgtatgcc
     7441 cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagct
     7501 gatggttctc tgcaaaaacc ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat
     7561 ggtaaaccgg cgctgttcac tcacgacgaa gtgatcaccc tgttccacga gttcggtcac
     7621 ggcctgcacc atatgctgac ccgcatcgaa accgctggtg tttccggtat cagcggtgtg
     7681 ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg ggagccggag
     7741 gcgctggcgt ttatctctgg tcactatgaa accggcgaac cgctgccgaa agagttgctg
     7801 gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg tcagctggag
     7861 ttcggcctgt ttgatttccg ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc
     7921 ctcgaaactc tggcagaaat caagaaactg gttgccgtgg tgccatctcc gtcctggggc
     7981 cgtttcccgc acgctttcag ccatattttc gccggtggtt atgcggcagg ttactacagc
     8041 tacctgtggg ctgacgtact ggcggcagat gctttctcgc gctttgagga agagggcatt
     8101 ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg cggttcagaa
     8161 gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg
     8221 gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg atgaaacagg
     8281 cgccggagac ggcgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     8341 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     8401 aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     8461 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     8521 ggatgtggtg gatgccactg caggactggg gcgcgatgcc tttgtgctgg cttcagtcgg
     8581 ttgccgcgtg cggatgctgg agcgtaatcc agttgttgcc gcgctgctcg acgacggcct
     8641 ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcgtt tgcagttaat
     8701 tcacgcctcc agcctgacgg cgctaaccga tattacccca cgcccgcagg tggtctatct
     8761 cgacccgatg ttcccgcata agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt
     8821 tcagtcgctg gtggggccgg atcttgatgc cgatggattg ctggagcctg cacgcttgct
     8881 ggcgaccaaa cgcgtggtag taaagcgccc ggactacgcg ccaccgctag cgaatgtcgc
     8941 cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag gtacgcccgt
     9001 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
     9061 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
     9121 cgcccagcgt aaccaggcca atcttaccaa acacgttggt atagacgggt aacgtctcaa
     9181 gcggatcggt aatgttgtcc ggcactgcgg taaatgttgc cacatagccg cccagcaaga
     9241 atgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
     9301 ccagggcagc aatcatcgcc aggccaaggg cgctaataaa caattcaccc aggctctgga
     9361 ataaatacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc gcaaaccaca
     9421 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
     9481 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
     9541 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gggtttatgg
     9601 aaaagccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gacgttggca
     9661 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
     9721 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
     9781 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccatgcac
     9841 atacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg aagctcatcg
     9901 gcttgaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
     9961 gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa ccgtatctat
    10021 cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga
    10081 acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc gaaagcaagc
    10141 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
    10201 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
    10261 ctccgagaac aatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
    10321 aaatgaggcc atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    10381 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    10441 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    10501 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    10561 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    10621 gtgaatagat taatacacta ttacaacaga aaataaccag ctgatcacac taatgatgga
    10681 aaatatgata gtgttaaatg ttgtattggc aggagatttt gattgcgtac gcagatcgta
    10741 ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc
    10801 gcatatgcgg gcgttcagga acacgtcgtg gggggatgat tattcttctt cgtcgcgcag
    10861 cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacattag
    10921 tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt atttcttgat
    10981 tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta
    11041 gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg
    11101 catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac
    11161 gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag
    11221 aactttgctt tccggggaga gatcgaccgc gatgagaatg tgtttataag ccatagtgtt
    11281 actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac
    11341 ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac
    11401 catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata
    11461 aacaaatttt cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg
    11521 atctcctaca ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca
    11581 ggacctggtg ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg
    11641 cgggcaggtc gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt
    11701 gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta
    11761 ctgcgtaact gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca
    11821 catggccaac ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc
    11881 gatcatcacg atgatgaatt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg
    11941 accagcgcat tgtgtggtct ggtggtggtc agcctgattg cattgatgat ttggcattaa
    12001 gcacttcagc aataataacg cataaaaaaa gcgggccagt aaactgaccc gctcaagact
    12061 gattacaaac gcaaaattgc ctgatgcgct acgcttatca ggcctacata atctctgcaa
    12121 tctattgaat ttgcatgttt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    12181 acaaagcgca ctatgtcaca atctgaagcg ggccagctga cccgctcgtt ttggtgcgta
    12241 cgattacagg aactgcaagg agagccagta cagcccgccg gaaagcagta ccgcagccgg
    12301 aagggtaaac acccaggcca tcagaatact ggttacggtt ttacgctgta agccgccgcc
    12361 atctaccacc atcgtccccg cgacagaaga ggagagtacg tgagtagtgg aaaccggcat
    12421 cccggtataa ctcgccaggc cgatagacac tgccgccgtc atctgggcag acatcccctg
    12481 agcgtaggtc atgcctttct taccgatttt ctcaccgata gtcgttgcca cacggcgcca
    12541 gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc
    12601 atactcgatg gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc
    12661 actcacgcca ggcatcttca ccactttgtc gatagtgtca gaaacgcaca gcataatgcg
    12721 gcgcatctgg ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtggtcaa
    12781 catacctttc aggcggttga gcgcgttaat ggtattcgac ggatggcagt ggaactccgc
    12841 aggttgcgtt gcgccagctt ccggagccgg tactaactga tcagcaccgg tagcctgttt
    12901 gagcagcgca ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg
    12961 ggtgatttcg tagccagtgg cattcatgtt caccacgaag cctgctggcg cgacgccaat
    13021 caataccaac ataaccagac caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc
    13081 cacgccgata gcggaaagga tcagcgcgat acgtgtccag aacggcggct ttttcttgcc
    13141 gtctttcttt tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct
    13201 ccagtagcga cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga
    13261 aacgatcaga gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga
    13321 cgtcccggtc atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg
    13381 agagctggat gcaggtaaac caaagtacca ggtacccagg ttccagataa tcgccgccag
    13441 caacatagag aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt
    13501 cggcagcata tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa
    13561 gttgaacacc gccgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac
    13621 ggttgccacg gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa
    13681 agccagtgca agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag
    13741 cataggacgt tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg
    13801 cgcgggcaaa gtgaaatata gacttttttt gatttgcctc ctgtatgga
//
LOCUS       DAZOYS010000104        13813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOYS010000104 DAZOYS010000000
VERSION     DAZOYS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.104"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(32..196)
                     /gene="yojO"
                     /locus_tag="ACHUN9_002661"
     CDS             complement(32..196)
                     /gene="yojO"
                     /locus_tag="ACHUN9_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0696017.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            305..799
                     /gene="napF"
                     /locus_tag="ACHUN9_002662"
     CDS             305..799
                     /gene="napF"
                     /locus_tag="ACHUN9_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0696018.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            789..1052
                     /gene="napD"
                     /locus_tag="ACHUN9_002663"
     CDS             789..1052
                     /gene="napD"
                     /locus_tag="ACHUN9_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0696019.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1049..3535
                     /gene="napA"
                     /locus_tag="ACHUN9_002664"
     CDS             1049..3535
                     /gene="napA"
                     /locus_tag="ACHUN9_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0696020.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3542..4237
                     /gene="napG"
                     /locus_tag="ACHUN9_002665"
     CDS             3542..4237
                     /gene="napG"
                     /locus_tag="ACHUN9_002665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0696021.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4224..5087
                     /gene="napH"
                     /locus_tag="ACHUN9_002666"
     CDS             4224..5087
                     /gene="napH"
                     /locus_tag="ACHUN9_002666"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0696022.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5084..5533
                     /gene="napB"
                     /locus_tag="ACHUN9_002667"
     CDS             5084..5533
                     /gene="napB"
                     /locus_tag="ACHUN9_002667"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0696023.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5543..6145
                     /gene="napC"
                     /locus_tag="ACHUN9_002668"
     CDS             5543..6145
                     /gene="napC"
                     /locus_tag="ACHUN9_002668"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0696024.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6158..6781
                     /gene="ccmA"
                     /locus_tag="ACHUN9_002669"
     CDS             6158..6781
                     /gene="ccmA"
                     /locus_tag="ACHUN9_002669"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0696025.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6778..7440
                     /gene="ccmB"
                     /locus_tag="ACHUN9_002670"
     CDS             6778..7440
                     /gene="ccmB"
                     /locus_tag="ACHUN9_002670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0696026.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7482..8219
                     /gene="ccmC"
                     /locus_tag="ACHUN9_002671"
     CDS             7482..8219
                     /gene="ccmC"
                     /locus_tag="ACHUN9_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0696027.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8216..8425
                     /gene="ccmD"
                     /locus_tag="ACHUN9_002672"
     CDS             8216..8425
                     /gene="ccmD"
                     /locus_tag="ACHUN9_002672"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0696028.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8422..8901
                     /gene="ccmE"
                     /locus_tag="ACHUN9_002673"
     CDS             8422..8901
                     /gene="ccmE"
                     /locus_tag="ACHUN9_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0696029.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8898..10841
                     /gene="ccmF"
                     /locus_tag="ACHUN9_002674"
     CDS             8898..10841
                     /gene="ccmF"
                     /locus_tag="ACHUN9_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0696030.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10838..11395
                     /gene="dsbE"
                     /locus_tag="ACHUN9_002675"
     CDS             10838..11395
                     /gene="dsbE"
                     /locus_tag="ACHUN9_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0696031.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11392..12444
                     /gene="ccmH"
                     /locus_tag="ACHUN9_002676"
     CDS             11392..12444
                     /gene="ccmH"
                     /locus_tag="ACHUN9_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0696032.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12479..13126)
                     /gene="narP"
                     /locus_tag="ACHUN9_002677"
     CDS             complement(12479..13126)
                     /gene="narP"
                     /locus_tag="ACHUN9_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0696033.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13446..>13813
                     /locus_tag="ACHUN9_002678"
     CDS             13446..>13813
                     /locus_tag="ACHUN9_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0696034.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2959 a         3474 c         4049 g         3331 t
ORIGIN      
        1 cattcagaca attttacctg ctcttttagt gttaaattct gatgagggag caaaccagca
       61 gaaatatctc tggtgaatta atcgctatat aaatatgttt ataaccattt gaaatgtgag
      121 caaaagcccg tttttgccac gcttcccgcc actcttttga tcctgctaca ggttttaccc
      181 cgatcggggt atgcatcttt gacacatcct ttaatatctt agcggctata aaaatggctt
      241 attaattatg cggtttattt ggtcgctctc aattttcaga gcgcgttaat gatggaaggt
      301 caatgtgaag attgatgcat cccgtcgggg catactcact ggtcgctggc gcaaagccag
      361 taacggtatc cgtccgccct ggtccggtga tgaatctcat tttctgaccc attgcacccg
      421 ttgcgacgcc tgtattaatg cttgtgaaaa caacattctg caacgcggcg cgggcggcta
      481 tccgagcgtt aatttcaaaa ataacgagtg cagcttctgt tacgcctgcg cgcaggcttg
      541 ccccgaatcg ctattttctc cgcgccacac cagggcctgg gatttgcagt tcaccattgg
      601 ggacgcgtgc ctggcgtatc agtcagttga atgccgccgc tgtcaggata gctgtgagcc
      661 aatggcaatt atctttcgcc cgacgctctc cgggatctac cagccgcaac ttaatagcca
      721 actttgcaac ggatgtggag catgtgcagc cagctgtccg gtatcagcca taactgcgga
      781 gtatctccat gcacactaac tggcaagttt gcagcctggt cgtgcaggcc aaaagcgaac
      841 gaatttcaga catcagcacc caactgaacg cctttcccgg ctgtgaagtt gctgtcagcg
      901 acgcgccgag cggtcagttg attgtggtgg tggaagcaga agacagcgaa acgctgatcc
      961 aaaccattga gtcagtacgc aacgtagagg gcgtgctggc ggtgtcgctg gtttatcacc
     1021 agcaggaaga gcaaggtgag gaaacaccat gaaactcagt cgtcgtagct ttatgaaagc
     1081 taacgccgtt gcggccgctg cggcggctgc cggtctcagc gtgccgggcg ttgcccgcgc
     1141 cgttgttggt cagcaggaag ccattaaatg ggataaagcg ccgtgccgtt tctgcggtac
     1201 tggttgcggc gttctggtcg gaacgcagca ggggcgtgtg gtggcctgtc agggcgaccc
     1261 ggacgcaccg gttaaccgtg gcctgaactg cattaagggc tatttcctgc ccaagatcat
     1321 gtacggtaaa gaccgtttga cgcagccgct gctgcgtatg aaaaacggta aatatgacaa
     1381 agaaggcgaa tttaccccaa tcacctggga tcaggccttc gatgtgatgg aagagaaatt
     1441 caaaaccgcc ctgaaagaaa aagggccgga atcgatcggt atgttcggtt ctggtcagtg
     1501 gactatctgg gaaggttatg ccgcgtccaa gctgtttaaa gcgggcttcc gttcgaacaa
     1561 catcgacccg aacgcgcgtc actgtatggc gtcggcagta gttggcttta tgcgtacctt
     1621 tggtatggat gagccgatgg gctgctatga cgacatcgag caggctgacg cgtttgtgct
     1681 gtggggcgca aacatggcgg agatgcaccc gatcctctgg tcgcgcatca ctaaccgtcg
     1741 tctctctaat cagaacgtca ccgtggcggt gctttctacc taccagcatc gtagcttcga
     1801 gctggcggat aacggcatca tctttacgcc acagtctgac ctggtgatcc tgaactacat
     1861 cgccaactat atcattcaaa acaatgcgat aaatcaggac ttcttcagca aacacgttaa
     1921 cctgcgcaaa ggggcgacgg acatcggcta cggtttacgt ccgacccatc cgctggaaaa
     1981 agcagcgaag aatccaggtt ctgacgcctc cgaaccgatg agctttgaag attacaaagc
     2041 cttcgttgcc gagtatacgc tggaaaaaac cgctgaaatg accggcgtgc cgaaagacca
     2101 gttagaacaa ctggcgcagc tgtatgccga tccgaacaag aaagtcatct cctactggac
     2161 gatgggcttc aaccagcata ctcgtggcgt gtgggctaac aacctggtct acaacctgca
     2221 cctgctgacc ggcaaaattt cccagccggg ttgcggtccg ttctccctga ccgggcaacc
     2281 ttctgcgtgt ggtactgcgc gtgaagtggg cacctttgct caccgtctgc ctgcggacat
     2341 ggtggtgacc aacgagaaac accgtgatat ctgcgagaag aagtggaata tcccgagcgg
     2401 caccattccg gcgaaaatcg gtctgcacgc ggtggcacaa gaccgtgcgc tgaaagacgg
     2461 caaactgaat gtttactgga ccatgtgtac caacaacatg caggccgggc cgaacatcaa
     2521 tgaagagcgt atgccgggct ggcgcgatcc gcgcaacttc atcatcgtct ccgatccgta
     2581 tccgacagtc agtgcgctgg ccgccgactt gatcctgccg accgcaatgt gggtagagaa
     2641 agagggtgct tacggtaacg ccgaacgccg tactcagttc tggcgtcagc aggtacaggc
     2701 accgggcgaa gcgaaatcgg atctctggca gttagtccag ttctcccgcc gcttcaaaac
     2761 tgaagaagtg tggccggaag agctgctagc gaagaaaccg gaactgcgtg gcaaaacgct
     2821 gtacgaagtg ctttatgcca caccggaagt gagcaaattc ccggtatccg aactggcgga
     2881 agatcagctg aacgatgaat cccgcgagct gggcttctat ctgcaaaaag ggctgttcga
     2941 agagtacgca tggtttggtc gcggtcacgg tcacgatctc gcgccgttcg atgactacca
     3001 caaagcgcgc ggtctgcgct ggccggtggt gaacggtaaa gaaacgcagt ggcgttacag
     3061 cgaaggtaac gacccgtacg tgaaagcggg cgaaggctat aagttctacg gtaaaccgga
     3121 tggtaaagcg gtgatcttcg cgctgccgtt cgaaccggcg gcggaagcac cggatgaaga
     3181 gtacgacctg tggctctcta ccggacgcgt tctggagcac tggcacactg gcagtatgac
     3241 tcgccgtgtg ccggaactgc accgcgcctt cccggaagcg gtcttgttta ttcacccgct
     3301 ggatgcgaaa gcgcgcgatc tgcgccgtgg cgacaaagtg aaagtggttt ctcgccgtgg
     3361 cgaagtgatc tcgattgttg aaacgcgcgg tcgtaaccgt ccgccacagg gcctggtgta
     3421 catgccgttc ttcgacgccg cacagctggt taacaaacta acgctggacg cgaccgatcc
     3481 gctctcgaaa gagacggact tcaagaagtg cgcagtcaaa ctggagaagg tgtaagccat
     3541 tatgtcccgg tcagcgaaac ctcaaaatgg tcgccgccgc tttctgcgcg atgttgttcg
     3601 cacagcaggc gggctggctg ccgtgggtgt ggcgctgggg ttacaacagc aaaccgcacg
     3661 cgcatctggc gtgcggttgc gcccgcccgg agccataaac gagaacgcct ttgccagtgc
     3721 ctgtgtgcgt tgcggtcagt gtgttcaggc ttgcccttac gacaccttaa aactggcgac
     3781 gctggcttct ggcctgtcgg cgggcacgcc atattttgtc gcacgggata ttccttgcga
     3841 aatgtgtgag gacattccgt gcgccaaagt gtgcccgagt ggtgcgctgg atcgtgagat
     3901 cgaatcgatc gacgacgcgc ggatggggct ggcggtgctg gtggaccagg aaaactgtct
     3961 caactttcag gggctgcgct gcgatgtttg ttatcgcgaa tgcccgaaaa ttgatgaggc
     4021 catcaccctg gagctggagc gcaacacgcg taccggtaag cacgcccgct ttctgccgac
     4081 ggttcacagc gacgcctgta ccggctgcgg taagtgcgaa aaagtgtgcg tgctggaaca
     4141 accggcaatc aaggtgttac cgctgtcact ggcgaaaggg gagttaggtc accattaccg
     4201 cttcggctgg ctggagggga acaatggcaa atcgtaaacg tgacgccggg cgcgaggcgc
     4261 tggtgaaaaa aggctggtgg cgcagtcacc gttggctggt gttacgtcgc ctttgtcagt
     4321 tcttcgtgct ggggatgttt ttgagcggtc cgtggtttgg tgtgtggatc ttacacggca
     4381 actacagctg tagcctgtta ttcgacaccg ttccgctgac cgatccgctg atgacgctgc
     4441 aaagtcttgc cagtggtcat ctgcctgcca cggtggcgtt gaccggtgcg gtgataatca
     4501 ctgtgctcta cgccctggca ggaaagcgat tattttgcag ctgggtctgc ccgctgaacc
     4561 cgattaccga cttagcgaac tggttacgca ggcgctttga cctcaatcag tctgcgacga
     4621 tccctcgcca cattcgctac gtgctgttgg tggtcattct gctgggatca gccttaaccg
     4681 gcacgctcat ctgggaatgg atcaaccccg tttccctgat ggggcgtagc ctggtaatgg
     4741 gcttcggcag cggcgcgctg cttattctcg cgctgttttt atttgatcta ctggtcgttg
     4801 aacacggctg gtgcgggcac atttgtccgg ttggcgcgct gtatggcgtg ctgggtagca
     4861 aaggtgtgat taccgttgcg gccagcgatc ggcagaaatg taaccgctgt atggattgtt
     4921 ttcatgtttg cccggaaccg catgtgctac gtgccccggt gctggatgaa caaagcccgg
     4981 tgcaggtcac cagccgcgat tgcatgacgt gcggtcgctg cgtggatgtc tgttctgagg
     5041 atgtatttac aataactaca cgatggagtt cgggagcgaa atcatgaaaa gccatgacct
     5101 gaagaaagcg ctgtgtcaat ggacggcgat gctggccctg gtggtaagcg gcgcggtttg
     5161 ggcggctaat ggcgtcgatt ttagccagtc gccggaagta tccgggacgc aggaaggggc
     5221 cattcggatg ccaaaagagc aggatcggat gccgctgaac tatgtgaatc agccgccaat
     5281 gatcccgcat agcgttgaag gttatcaggt aacgaccaat accaaccgct gcttgcagtg
     5341 ccacggtgtc gaaagctatc gcaccactgg cgcgccgcgt atcagtccta ctcactttat
     5401 ggacagcgac ggcaaagtgg gcgcggaagt ggctccgcgt cgttatttct gtctgcaatg
     5461 tcacgtaccg caggccgata ccgcgccaat cgtggggaat acctttaccc catcaaaagg
     5521 ttacgggaaa taagaggtta ttatgggaaa ttctgaccgt aagcctggtc tgattaagcg
     5581 cctgtggaaa tggtggcgta cccccagccg tctggcgctg ggaacgctgc tgttgatcgg
     5641 ttttgtcggc ggcatcgtct tctggggcgg ctttaacacc ggaatggaaa aagccaatac
     5701 cgaagagttc tgcattagct gccacgaaat gcgtaacacg gtgtatcagg aatacatgga
     5761 ttccgtgcac tacaacaacc gtagcggcgt ccgtgcgacc tgtccggatt gtcacgttcc
     5821 gcacgagttt gtgccgaaga tgatacgcaa gctcaaagca agtaaagagc tgtatggtaa
     5881 aatttttggc gttattgaca cgccgcagaa atttgaagct catcgtctga caatggcaca
     5941 gaatgagtgg cggcgcatga aggacaataa ctcgcaggag tgccgtaact gtcacaactt
     6001 cgagtatatg gatacaaccg cccagaaatc ggttgccgcg aagatgcatg accaggcggt
     6061 gaaagatggg caaacctgta ttgattgcca taaagggata gcgcacaagc tgcccgatat
     6121 gcgtgaagtc gagccaggtt tttaacaggg ttattgcgtg ggtatgcttg aagccagaga
     6181 gttactttgt gagcgggatg aacgaacctt atttagtggc ttgtcattta cgctgaacgc
     6241 aggagagtgg gtacaaatca ccggtagcaa cggcgcgggg aagacaacgc ttctccgttt
     6301 gctgacgggg ttgtctcgcc ctgacgcagg cgaggttctc tggcaagggc agcccttgca
     6361 tcaggtacgc gacagctacc atcaaaacct gttatggata ggccatcagc cggggatcaa
     6421 aacccggctg acggcgttag aaaatctgca cttttatcat cgcgatggcg ataccgcaca
     6481 atgtctggaa gccctggcgc aggccgggct tgccggattc gacgatattc cggtaaatca
     6541 actttctgcc gggcaacaac gccgcgtcgc tttagcgcgt ctgtggctga cccgtgccac
     6601 gttatggatc ctcgacgagc cttttaccgc gattgacgtt aacggcgtcg atcgtctgac
     6661 ccagcgtatg gcgcagcata cggagcaggg ggggattgtg attctgacta cccaccagcc
     6721 gctcaacgtt gctgaaagta aaattcgccg catttcactg acgcaaacga gggccgcatg
     6781 atgttctggc gcattttccg tcttgagctg cgtgtagcgt ttcgccatag cgccgaaatc
     6841 gccaacccgc tgtggttctt cctgattgta attacccttt ttccgctcag tatcggtccg
     6901 gagccgcaac tgctggcgcg tattgcaccg ggcattatct gggttgctgc gctgctttca
     6961 tccttgctgg cgctggaacg actgttccgt gacgatttgc aggacggcag tcttgaacaa
     7021 ttgatgttgt tgccgttacc cttgcccgcc gttgtgctgg cgaaggtgat ggcgcactgg
     7081 atggtaaccg gtctgccgtt actcatcctt tcgccactgg tagcaatgct actgggaatg
     7141 gatgtttatg gctggcaagt gatggcgctg acgctgctgc tgggaacgcc tacgcttggc
     7201 tttctcggtg caccgggtgt ggcgttgaca gtgggactta agcgcggtgg tgtgctgctc
     7261 agcatactgg tgttaccgct gactatccca ttactcatct ttgccaccgc cgcgatggac
     7321 gcggcttcca tgcatttgcc cgttgacggg tatctggcaa ttttaggcgc gttgctggca
     7381 ggcaccgcga cattaagtcc ttttgcgacg gcggcagcgt tacgaatcag cattcaataa
     7441 cgctggatta ctgatttttg ttcgagtctg gtatcgaaac tatgtggaaa acactgcatc
     7501 aactggcgat cccaccacgg ctgtatcaaa tctgtggctg gtttataccg tggctggcaa
     7561 ttgccagtgt ggtcgtgctt accgtcggct ggatctgggg attcggcttt gctccggctg
     7621 attatcagca gggaaatagc taccgcatta tctacctgca tgtgcctgcg gcgatctggt
     7681 cgatgggcat ttatgcatca atggcagtgg cagcgtttat tggccttgtc tggcagatga
     7741 aaatggccaa cctggcggtg gcggcgatgg cccccattgg tgccgtgttt acctttattg
     7801 ccctggttac cggctctgca tggggaaaac cgatgtgggg cacctggtgg gtatgggatg
     7861 cacgtctgac ttctgaactg gtgctgctgt ttttgtatgt gggtgtgatt gccctgtggc
     7921 acgccttcga cgaccgccgt ctggcgggcc gtgcggcagg tatcctggtg ctgattggcg
     7981 tggtgaatct gccgattatt cattactccg tggagtggtg gaacaccctg catcagggat
     8041 caacgcggat gcagcaaagt atcgatccgg cgatgcgttc gccgctgcgc tggtcgattt
     8101 ttggcttcct gctcctgtct gccacgctga cgctgatgcg gatgcgtaat ttgattttgc
     8161 tgatggaaaa acgccgtccg tgggtgagtg aactgatact gaaaagaggc cgtaaatgac
     8221 ccctgcattt gcttcctgga atgaattttt cgcaatgggc ggttacgcct tttttgtctg
     8281 gctggcggtg gtgatgaccg ttattccgct ggtggttttg gtcgtgcact cggtgatgca
     8341 acatcgcgca attctgcgtg gtgtggcgca acagcgggcg cgtgaggcgc gtttacgtgc
     8401 tgcgcaacag caggaggctg catgaatatt cgccgtaaaa accgcttgtg gattgcctgt
     8461 gccgtgttgg cagggctggc gctgactatc ggtctggtgc tatatgcgct gcgctcgaat
     8521 atcgatctct tttatacgcc gggggaaatt ctctacggca agcgtgaaac tcagcaaatg
     8581 ccggaagtcg gtcagcgtct gcgcgttggc gggatggtga tgctgggtag tgtgcagcgc
     8641 gatcccaatt cgctgaaagt gaccttcact atttacgatg ctgaaggctc agtggatgtc
     8701 tcttacgaag gcattttgcc ggatctgttc cgtgaagggc agggcgttgt ggtgcagggc
     8761 gagctggaaa aaggcaatca tatcctcgcg aaagaagtgc tggcgaaaca cgacgaaaac
     8821 tacacgccgc cagaagttga gaaagcgatg gaagctaacc accgtcgccc ggcgagtgtt
     8881 tataaggacc cagcatcatg atgccggaaa ttggaaacgg gctgctgtgt ctggcgttag
     8941 ggattgcgct gctgctgtcc gtgtatccgc tatggggcgt ggcgcgcgga gatgcgcgca
     9001 tgatcgcgtc ttcccgcttg tttgcctggc tgctgtttat gtctgtggct ggcgcatttc
     9061 tggtgctggt caatgctttc gtggtcaacg actttaccgt cacctatgtt gccagctact
     9121 ccaataccca gcttccggtg tggtatcgcg tggcggctac ctggggcgcg catgaaggct
     9181 cgctactgct gtgggtgctg ctgatgagcg gctggacctt tgcggtggcg atttttagtc
     9241 agcgtattcc gctggatatt gtggcccgcg tactggcgat aatggggatg gtcagcgtcg
     9301 gctttttgct gttcattctc tttacctcta acccattctc acgcacgctg ccgaacttcc
     9361 cgattgaagg gcgcgatctt aacccgctgt tacaggaccc ggggctgatc ttccatccgc
     9421 ctctgcttta tatggggtac gtgggtttct cggtggcgtt tgcttttgcc attgcttctt
     9481 tgctgagcgg gcgtctggac agcacttatg cgcgttttac tcgcccgtgg acgctggcgg
     9541 cgtggatttt cctgacgctc ggcattgtgc tcggttccgc atgggcctat tacgaactcg
     9601 gctggggggg ctggtggttc tgggatccgg tagaaaacgc ctcgtttatg ccgtggctgg
     9661 tggggactgc gctgatgcac tcactggcgg tcactgaaca acgcgccagc ttcaaagcgt
     9721 ggacattact gctggcaatc agtgccttct cgttgtgtct gttggggact ttcctcgtgc
     9781 gttccggcgt gctggtatcg gtacacgcgt ttgcgtctga tccggcgcgc ggtatgttta
     9841 tcctcgcctt tatggtgctg gtgattggag gttcgctgct gctgtttgcc gcgcgtggac
     9901 acaaagttcg ctcacgcgta aacaatgcgc tgtggtcgcg ggaatctctg ctgttagcga
     9961 acaacgtttt gctggtcgcc gcgatgctgg tggtgttgct ggggacgctg ctgccgctgg
    10021 tgcacaagca actgggactg ggcagtattt cgattggcga accgttcttc aacaccatgt
    10081 ttacctggct gatggtgccg tttgcgctac tgctgggtgt cggtcctctg gtgcgctggg
    10141 ggcgcgatcg cccacgtaaa atccgcaatt tattgattat cgccttcatc tcaacgctgg
    10201 tgctgtctct gctgttgccg tggctgtttg agagcaaagt tgtggcgatg acggtgctcg
    10261 gcctggcaat ggcctgctgg attgcggtgc tggcaattgc ggaagctgcg ctgcgtattt
    10321 cacgcggcac gaaaaccacc ttcagttatt gggggatggt ggctgctcac ctggggctgg
    10381 cagtgacaat tgttggcatt gcttttagcc agaactatag cgttgagcgt gatgtgcgca
    10441 tgaagtccgg cgatagcgtc gatattcatg aatatcgctt caccttccgt gatgtcaaag
    10501 aggtgactgg cccgaactgg cgtggcggtg tggcgactat cggcgtaacg cgagagggta
    10561 aaccggaaac agtgctgtat gcggaaaaac gttattacaa cactgccggg tcgatgatga
    10621 ccgaagcggc gattgacggt ggcatcacgc gtgacctgta cgccgcgctc ggtgaagagc
    10681 tggaaaacgg cgcgtgggct gtgcgtcttt actacaaacc atttgttcgc tggatttggg
    10741 cgggcgggct gatgatggcg ttgggcggac tgctgtgtct gtttgatcct cgctatcgta
    10801 agcgcgtgag tccgcaaaaa actgcgccgg aggctgtatg aagcgcaaag tattgttaat
    10861 tccgttgatt atcttcctgg cgattgccgc ggcgctgctg tggcagctgg cgcgtaatgc
    10921 cgaaggggat gatccgacca atctggaatc ggcgctcatt ggcaagccag tgccgaagtt
    10981 tcgcctcgaa tcactggaca atccggggca gttttatcag gcggatgtgc tgactcaggg
    11041 caaaccagtg ctgcttaacg tctgggcgac ctggtgtccg acctgccgtg cggaacatca
    11101 atatctgaat cagctttctg cgcagggcat ccgcgtagtc ggcatgaact ataaagacga
    11161 tcgccagaag gcaatcagct ggctgaaaga gctgggcaat ccttacgcgc taagcctgtt
    11221 tgatggcgat ggcatgttag ggctggatct cggtgtctat ggcgcgccag aaacgttcct
    11281 tattgacggc aacggcatca ttcgctatcg ccatgcgggc gatctcaatc ctcgcgtctg
    11341 ggaagaagag atcaagccgc tgtgggagaa atacagtaag gaggccgcac aatgaggttt
    11401 ttattgggca tgctgatgct gatgatctcc ggctcagcgc tggcgaccat cgacgtgttg
    11461 cagtttaaag atgaagcaca ggaacaacag ttccgtcagc tcactgaaga actgcgctgc
    11521 ccgaaatgcc agaacaacag cattgccgat tccaactcga tgattgccac cgacctgcgc
    11581 cagaaagtgt atgaactgat gcaggaaggt aaaagtaaaa aagagattgt cgattatatg
    11641 gtggcgcgtt acggcaactt cgtcacttac gatccgccgt taacgccgct gaccgtgctg
    11701 ctgtgggtgc tgccagtagt agctattggc attggcggtt gggtcatata cgcccgctcg
    11761 cggcgtcggg tacgcgtggt gccggaagcg tttcctgaac aaagcgtgcc ggaaggtaag
    11821 cgtgccggat atgtcgctta tttgccgggc attgtggtgg cgttaattgt ggctggcgtc
    11881 agctactacc agaccggaaa ttatcaacag gtgaaaatct ggcagcaggc cacggcacag
    11941 gctccggcgt tgctggacag ggcgctggat ccgaaagctg atccgctcaa cgaagaagag
    12001 atgtcgcgcc ttgcgctggg gatgcgtact caactgcaaa aaaatccggg agatatagac
    12061 ggctggatta tgttgggccg cgttggcatg gcgctgggta acgccagtat cgccaccgat
    12121 gcatacgcta ctgcgtatcg cctcgatccg aaaaacagtg atgccgcact gggttatgct
    12181 gaagcgttga cacgttcatc tgatcccaac gacaaccgcc tcggcggcga actgctacgt
    12241 cagctggtga gaacggacca tagcaatatc cgtgtgctaa gcatgtatgc gtttaatgcc
    12301 tttgagcagc agcgatttgg cgaagccgtt gccgcgtggg agatgatgtt gaaactctta
    12361 cctgccaacg atactcgccg tgcggtgatt gaacgtagta tcgcgcaggc gatgcaacat
    12421 ttgtcgccgc aggagagtaa ataaaaagag gcccgatggt gatgccatcg ggctattttt
    12481 attgtgcccc gcgttgttgc aggaacagaa tggtggccgc cacgcgtgag cggacattga
    12541 gtttacgcag cagatttcga atatgaactt ttactgtctg ctcggaaata ttcaacacag
    12601 aggcaatctg tttatttgac agcccctgtg ccagctcgtg cagaacatcc agctcgcgct
    12661 ccgtcagcac gctgaaggga tcttcttccg cgccaaacat ttcacgttca cgtaagtact
    12721 gattgacgcg ttcgctaaag actttgctgc ctttcgctcc ggcacgaatc gcttccagca
    12781 acacttccgg gtcgctgtct ttcagcagat aaccgtctgc gcctgcgtct atcagcgcaa
    12841 agacatcgct ggaggcatcg gatacggtca ggataataat ttgcgcggta acgccatccc
    12901 tgcgcaaggc attgagggta tccaggccac tcataccttt catattgaga tccagcaaga
    12961 tcacgtcgat atccagtcta ttcgccagat cgatcgcgct cgcgccgtcg cccgcttcgg
    13021 cgaccacttc aaagccagga tcaagctcca gtaactgacg aacaccgcgt cgcataagtg
    13081 gatgatcatc cacaatcatc acctgaaaag gcgttgcttc aggcatagta gtctcctgag
    13141 gttttattag aatcattatg tttgagcgta gcgttggtaa cgctaccagg cgcgtgaatg
    13201 ttaccccatt ttatgtatgg acatgtagtg aaaactacaa ccttgccgtt cgctttggca
    13261 tcattttcat tataaagacg gaattttgca tcagcttctc atcaagaaaa acaactaaaa
    13321 aatcaatcac aatacttgtg catttcttta aacagttttt aaaaactgac tccgggtatg
    13381 gagctatggg tattttctgt acccaatgct tttaacagca attaatttca taggatgaaa
    13441 gctcaatgca tcaatctggt tctgtttctc tttgtcgttc cgcaatatct gttctggtgg
    13501 ctacagcgtt atattcaccc atagcattgg catcaacagt tgagtatggt gagacagttg
    13561 atggtgttgg cctggaaaaa gatatccagc tggtttatgg gaccgccaat aatacgaaaa
    13621 tcaatcctgg cggagaacag catataaaag aatttggtgt aagtagtaat actgaaatta
    13681 acggcgggta tcagtacatt gaaatgaatg gcactgcaga atactcagta ttaaatgatg
    13741 gttatcaaat tgttcaaatg ggtggcgcgg taaaccagac tacgctcaat aatggagtgc
    13801 tacaggttta tgg
//
LOCUS       DAZOYS010000105        13770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOYS010000105 DAZOYS010000000
VERSION     DAZOYS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.105"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            62..787
                     /locus_tag="ACHUN9_002679"
     CDS             62..787
                     /locus_tag="ACHUN9_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0696035.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1280..2026
                     /gene="mipA"
                     /locus_tag="ACHUN9_002680"
     CDS             1280..2026
                     /gene="mipA"
                     /locus_tag="ACHUN9_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0696036.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTILAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2116..2970
                     /gene="yeaE"
                     /locus_tag="ACHUN9_002681"
     CDS             2116..2970
                     /gene="yeaE"
                     /locus_tag="ACHUN9_002681"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0696037.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3021..3905)
                     /locus_tag="ACHUN9_002682"
     CDS             complement(3021..3905)
                     /locus_tag="ACHUN9_002682"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0696038.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(3989..4984)
                     /gene="gapA"
                     /locus_tag="ACHUN9_002683"
     CDS             complement(3989..4984)
                     /gene="gapA"
                     /locus_tag="ACHUN9_002683"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0696039.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5326..5739
                     /gene="msrB"
                     /locus_tag="ACHUN9_002684"
     CDS             5326..5739
                     /gene="msrB"
                     /locus_tag="ACHUN9_002684"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0696040.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5781..6053
                     /locus_tag="ACHUN9_002685"
     CDS             5781..6053
                     /locus_tag="ACHUN9_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0696041.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6214..6855)
                     /gene="pncA"
                     /locus_tag="ACHUN9_002686"
     CDS             complement(6214..6855)
                     /gene="pncA"
                     /locus_tag="ACHUN9_002686"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0696042.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(6866..7882)
                     /gene="ansA"
                     /locus_tag="ACHUN9_002687"
     CDS             complement(6866..7882)
                     /gene="ansA"
                     /locus_tag="ACHUN9_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0696043.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8049..9905)
                     /gene="sppA"
                     /locus_tag="ACHUN9_002688"
     CDS             complement(8049..9905)
                     /gene="sppA"
                     /locus_tag="ACHUN9_002688"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0696044.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10066..10617
                     /locus_tag="ACHUN9_002689"
     CDS             10066..10617
                     /locus_tag="ACHUN9_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0696045.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10734..11777
                     /gene="selD"
                     /locus_tag="ACHUN9_002690"
     CDS             10734..11777
                     /gene="selD"
                     /locus_tag="ACHUN9_002690"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0696046.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            11782..13743
                     /gene="topB"
                     /locus_tag="ACHUN9_002691"
     CDS             11782..13743
                     /gene="topB"
                     /locus_tag="ACHUN9_002691"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0696047.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3507 a         3338 c         3598 g         3327 t
ORIGIN      
        1 gattaaaaat attcctttct tgtccataaa taccccgtcg tatttttaaa aaaggtatct
       61 tatggcaagg ataagtaaaa aggaatctgc taatcacaat aaagtgatgg aattaataca
      121 ttcagataag ctcctaacac aagaagatag ggaattcata ttcaccaatt atagggggga
      181 tgggataggg gctacaggtg ccttcttcac acctgaaatt ttagcatggg attttgtact
      241 tgatgctgac tgtactggtg actgtattga actatgtgct ggtatcggtc gcctcagcta
      301 ctaccagtac ttaaggaata aaccaacgca tattacatgc gtggaactca atcctgagta
      361 cgtaatgatc gggaagaggg tactaccaga ggcagaatgg atcactggta acgcactaca
      421 gtactcacca gaccgtttct atgatgttgc ctatggtaat ccaccctttg ggaagatcag
      481 tacctcaaaa gcgtatacag gccgttataa gggtagtgaa tttgaataca aggtgattga
      541 acatgcaagt acctttgcgt cctatggtgc atggatagta ccgcaaggtt ccgcaggctt
      601 tgtctacagt ggggtacatt gttatgagcg tagagaatca tgtaagtacc agaagtttgt
      661 gaaggatact ggctatgagc tacatccagg tgtaggcatc gatacaagta tctacaggga
      721 tgaatggaat ggtactaagg tgatatgcga atcggtgtta gtagatgatg acggtacaaa
      781 tctgtaacta aattagttaa gaaatcataa agatagtagt acttgcaaat gttcccattg
      841 atgaagttat gcgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta
      901 gaggctcccg gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg
      961 ccattttatc gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga
     1021 tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca
     1081 tccataaatt ttgcataatt aatgtaaaga caaggctcgc cagtaacgct aaattcattt
     1141 ggctgtaagc gcggtgtcat ccgcgtcagg aaaattaaac agttacttta aaaaatgaaa
     1201 acgtaaaaag gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt
     1261 gtttaaggaa tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc
     1321 aacgtctgca ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtcggtgt
     1381 cgttgaacac ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta
     1441 tgaaggcgat aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc
     1501 aacggacaaa ctttcaatta ccgcttattg gtcgccgctt tacttcaaag cgaaagacag
     1561 tggcgatcac caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc
     1621 ttatgctcac tttacccagt acggttacct gcgtaccatc ctggctggcg ataccctgga
     1681 taacagcaac ggcattgtct gggatatggc ctggttgtat cgttacacca acggcggcct
     1741 gacagtgacc ccgggtatcg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta
     1801 tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaacgacag
     1861 ctggagccct tacctggagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta
     1921 cggtaccgcg cgctataccc gtctgtctga tgaagttact gacagcccga tggtggataa
     1981 atcctggact ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc
     2041 agggcatttt gtgcataaaa acagggcgtc acgccctgtt ttgcattacg gtggtgcaat
     2101 acctgggagg aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca
     2161 gccatagggc agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt
     2221 gctgcactac gcgcgggcat tgagctcggt ttaaccctca ttgataccgc cgaaatgtat
     2281 gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg tgataacgtc
     2341 tttctcgtct ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc
     2401 gaagccagtt tacgccgtct caatactgat tatctcgatc tctacttatt acacaggtct
     2461 ggcagtttcg cttttgaaga gactgttgca gcgatggaaa aattgatcgc ccagggaaaa
     2521 atccgccgct ggggcgtttc taaccttgat tatgctgata tgcaggaact ctggcagctg
     2581 ccggggggaa atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt
     2641 gagtacgatc tactcccctg gtgccagcaa cagcagatgc cggtgatggc ttacagtcca
     2701 ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt caacgaaatt
     2761 gcacatgctc acaatatcag ctcggcacaa gtattgttgg cgtgggtaat cagccatcag
     2821 ggtgtgatgg cgattccaaa agcggccacg attgcccatg ttcaacagaa tgcggctgtg
     2881 cttgaggtcg aactttcgtc agcggaatta accatgctgg ataaagcgta tccggcacca
     2941 aaaggaaaaa ctgcgctgga tatggtgtga tgttgatacg tgccggatgc atcgcacccg
     3001 gcacacaaca ttaacgcaaa ttaacgtttc gcaacgcgaa tggattgcgc cagatgtgca
     3061 ggtttctctt tggtgacttt ttgcgtttct gaagcgtaag ccgtttctac acaaacaaat
     3121 gttttgtagc catcatccgg catatcgccc atgctaattg aaagcgccgg tcccgggttc
     3181 cagcctacaa cgttcagatg atgctggtgg cctacggcga taatacgatt cagcgcttca
     3241 tcattaatca cgctgcaatc ttgcggattc agatacacgc gatcggtacg gtcagggaag
     3301 gtctgaatac cgtcggtcag tacatcttct ttcgcgtcat tcactttatc aatgaagcga
     3361 tcgcccagcc cactgacgct taccttagcg atatcaccca cgttaaagta ggtatgcagg
     3421 gcagaggtgg tttcaaattc gccatgtgat tcaagatcga tttcacaggt tttacccacg
     3481 cggaaatgcg ctaacagcgt aaagtcgtgc ggacagaatt ttttcgtctc ttcgctttgc
     3541 gtcaattcaa aagtcagcgc tacgccatca gcatcttcat tatgtgattt cagcgtccac
     3601 ggcaggttgc gggcaaaacc gtgcgcaggc agaccttgtt gtgccgccgg accaaaccac
     3661 ggccagcaaa ccggtacgcc accgcgaata gcgacgccat ttttgaacgg tgtgttgttg
     3721 ctcaaccaca gaacttcttc ttcacccgca ggtttccacg agagaaggtg tgcgccctgt
     3781 aatgcaaaag aggcttttac ttggggatga tcgaccacaa tgaggtccag ttcatccagt
     3841 ttacgacggg agaggacagg ggagatttgt tcgatgaccg gaagggcaaa aattttctta
     3901 atcatgacgc agtcctttaa cttcatttta tcaggtaaaa aaaagagcga ccgaagtcgc
     3961 tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc aggtccagaa
     4021 ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg aagttgtcgt
     4081 tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg ttgaaatcgg
     4141 tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct tcagcagcgg
     4201 ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga acggtcaggt
     4261 caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt ttgccattca
     4321 gttctggcag tactttacct acagctttag cagcaccggt agaggacggg atgatgttct
     4381 gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg gttttctgag
     4441 tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag ttatcgttga
     4501 taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa acgatgtcct
     4561 ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg tctttagacg
     4621 gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca gtttcgtcag
     4681 tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc catttcaggt
     4741 tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc agatgaccgt
     4801 ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc agcatgtatg
     4861 ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca gaacgtttct
     4921 gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct actttgatag
     4981 tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa ttacaaaaac
     5041 cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc tgtgcctaag
     5101 cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg gggtcggcgc
     5161 cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt taggtgcagc
     5221 catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat gttttgttag
     5281 aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc taataaacct
     5341 tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac gcagaatcat
     5401 gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg cgtatatcac
     5461 tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc cggctgtggt
     5521 tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa agacttgtca
     5581 catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct ggggcatgtc
     5641 ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc ttctttacgc
     5701 ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc agcaacttat
     5761 tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg acgcctgagg
     5821 tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc gttgcgttga
     5881 ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct cgccataata
     5941 ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag agcaaacagc
     6001 agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag tgaatgagtt
     6061 atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga cgtcgtgttt
     6121 tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca atgtcattga
     6181 ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt cccagtctgc
     6241 cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg cactgtcctg
     6301 gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat aacctaactg
     6361 taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca taacgatcaa
     6421 ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc gacggccgtt
     6481 atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga acaccgctgc
     6541 gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct gcacacagtg
     6601 atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag gctctacacc
     6661 gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg cgataaccgc
     6721 ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg tactgtcacc
     6781 ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga ccagtaacag
     6841 ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg cgcaggtttt
     6901 ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg taatgcagtt
     6961 tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca tgggcgaggg
     7021 cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt gtcaggttga
     7081 ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct ttgttttgtg
     7141 gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg cgcagaaaat
     7201 tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt ggttgtggcg
     7261 tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta ttcaaacgac
     7321 gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac gcatcaaaac
     7381 catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg aaaaagagcg
     7441 ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga ttaatttgtc
     7501 cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc ggtttaccga
     7561 gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg gtgccgtgca
     7621 ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca atatgctgcc
     7681 agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga atggtgaaat
     7741 ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt agatgacctg
     7801 acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc gtgtaggcga
     7861 cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag caaaagtata
     7921 tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg gggcgagaca
     7981 gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc ggccactcag
     8041 tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt ttgtgggtcg
     8101 ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga ggcaagcggt
     8161 gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc agacatgttg
     8221 tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg ccactgtttc
     8281 acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag actatcgacc
     8341 agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat tttatcaatc
     8401 tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt atagccattc
     8461 tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt gatagaaaca
     8521 tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc cagactattt
     8581 tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct ggggttagcc
     8641 acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat gccgcccatc
     8701 gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg aatcacttca
     8761 gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt cactttcggg
     8821 tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt cccctgagtt
     8881 tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc accggtatct
     8941 gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt atcagtctta
     9001 ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc cagtgcatcg
     9061 accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt taaaccctca
     9121 agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt agcggcaaca
     9181 gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc gcgggctgcc
     9241 ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc cacgcggaac
     9301 acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc gttggtagca
     9361 aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc gaaactggcg
     9421 agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt cccgctgtca
     9481 cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc gcctgcgaag
     9541 tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg gcgaatggtg
     9601 ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc aagcagctgg
     9661 cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac accagaaatg
     9721 tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact gacctgcatc
     9781 cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac catttcacgg
     9841 acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg ccaaagggtt
     9901 cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat gtggttatcg
     9961 taatgaccca accggcactt gtcagcagga attgcggact gtgctgtaac aaaatccact
    10021 ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga tgcactcgaa
    10081 ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac gggtgaacaa
    10141 ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat gcaaccgtgg
    10201 catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact ggaacagggg
    10261 gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc gccgttccgc
    10321 gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt cccgcgatgg
    10381 gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc cgttgcccag
    10441 ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt gcgtgaagca
    10501 ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac gccgcagttg
    10561 aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta tttctgataa
    10621 ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac tctcacttat
    10681 cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg tccatgagcg
    10741 agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt aaaatttccc
    10801 caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat ccgaatttgc
    10861 ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat ggcaccagcg
    10921 ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat tttggccgca
    10981 ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg attatggcga
    11041 ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc gaagtaaccg
    11101 aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt cactccatcg
    11161 atgcgccgga gccgattttt ggtctggcgg taacggggat cgtaccgacc gagcgggtga
    11221 agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg ctggggatcg
    11281 gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag ggactagcga
    11341 cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc gaaggcgtaa
    11401 aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa atgtgtcagg
    11461 gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg ggggttgaag
    11521 agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc agctacggtc
    11581 atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg caaacttctg
    11641 gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct acagccgccg
    11701 agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc ggtcgtgcca
    11761 tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag tctggcgcgc
    11821 gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat cgagtgcggt
    11881 aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca gccagacgcc
    11941 tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattgtccc ggaaaagtgg
    12001 caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg cttcctgcat
    12061 gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact gctggtggat
    12121 gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca gcgttgcttg
    12181 ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg gtccaacagt
    12241 gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg gctgtacggc
    12301 atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca gggcgtactt
    12361 tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga tgaagagatt
    12421 gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac acctgccgat
    12481 gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca ggatgaagaa
    12541 gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag tggtcaacca
    12601 gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct gcctttttcg
    12661 ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca gaacgtgctt
    12721 gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg ttccgattgt
    12781 cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc catcagcgtt
    12841 catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa ccgctgttgg
    12901 gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag ttcagccatc
    12961 aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta cttgatgcag
    13021 ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc caaaggcaaa
    13081 tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt aggcagcaaa
    13141 gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg cgatgagttg
    13201 ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca ttttaccgat
    13261 gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa agatctgaaa
    13321 aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg gattattgaa
    13381 ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc taccgacgcc
    13441 ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat gaccgcgcac
    13501 tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga ctttatgcag
    13561 ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc ggtgcggcag
    13621 tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa ggctgcaccg
    13681 cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg ggcgatagcg
    13741 taagcgagtg aatctttcgt gctattcgag
//
LOCUS       DAZOYS010000106        13394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOYS010000106 DAZOYS010000000
VERSION     DAZOYS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.106"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(129..833)
                     /locus_tag="ACHUN9_002692"
     CDS             complement(129..833)
                     /locus_tag="ACHUN9_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0696048.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(970..1422)
                     /gene="moaE"
                     /locus_tag="ACHUN9_002693"
     CDS             complement(970..1422)
                     /gene="moaE"
                     /locus_tag="ACHUN9_002693"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0696049.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1424..1669)
                     /gene="moaD"
                     /locus_tag="ACHUN9_002694"
     CDS             complement(1424..1669)
                     /gene="moaD"
                     /locus_tag="ACHUN9_002694"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0696050.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1662..2147)
                     /gene="moaC"
                     /locus_tag="ACHUN9_002695"
     CDS             complement(1662..2147)
                     /gene="moaC"
                     /locus_tag="ACHUN9_002695"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0696051.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2150..2662)
                     /gene="moaB"
                     /locus_tag="ACHUN9_002696"
     CDS             complement(2150..2662)
                     /gene="moaB"
                     /locus_tag="ACHUN9_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0696052.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2684..3673)
                     /gene="moaA"
                     /locus_tag="ACHUN9_002697"
     CDS             complement(2684..3673)
                     /gene="moaA"
                     /locus_tag="ACHUN9_002697"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0696053.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3661..3805)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4070..4978
                     /gene="yvcK"
                     /locus_tag="ACHUN9_002698"
     CDS             4070..4978
                     /gene="yvcK"
                     /locus_tag="ACHUN9_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0696054.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5170..7191)
                     /gene="uvrB"
                     /locus_tag="ACHUN9_002699"
     CDS             complement(5170..7191)
                     /gene="uvrB"
                     /locus_tag="ACHUN9_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0696055.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7770..8438)
                     /gene="bioD"
                     /locus_tag="ACHUN9_002700"
     CDS             complement(7770..8438)
                     /gene="bioD"
                     /locus_tag="ACHUN9_002700"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0696056.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            complement(8431..9186)
                     /gene="bioC"
                     /locus_tag="ACHUN9_002701"
     CDS             complement(8431..9186)
                     /gene="bioC"
                     /locus_tag="ACHUN9_002701"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0696057.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9173..10327)
                     /gene="bioF"
                     /locus_tag="ACHUN9_002702"
     CDS             complement(9173..10327)
                     /gene="bioF"
                     /locus_tag="ACHUN9_002702"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0696058.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10324..11364)
                     /gene="bioB"
                     /locus_tag="ACHUN9_002703"
     CDS             complement(10324..11364)
                     /gene="bioB"
                     /locus_tag="ACHUN9_002703"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0696059.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11451..12740
                     /gene="bioA"
                     /locus_tag="ACHUN9_002704"
     CDS             11451..12740
                     /gene="bioA"
                     /locus_tag="ACHUN9_002704"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0696060.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12799..13275
                     /locus_tag="ACHUN9_002705"
     CDS             12799..13275
                     /locus_tag="ACHUN9_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0696061.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         3080 a         3655 c         3448 g         3211 t
ORIGIN      
        1 tatttatgga tacggaaaac gattccgctc gctcataagg catattttat acctggtact
       61 tgttaagtgt cgtttttagt aagaaaaaaa tcccgacaca tggccgggat agtgatggtg
      121 aaaaagaatt aacggcggtt gccgaagatc cgtaacaaca tcaggaacag gttgatgaag
      181 tccagataca aggttaacgc gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg
      241 gtatcaatct gctcacccat atttttcaat ttctgagtgt catacgccgt caatccgaca
      301 aagacaatca cgccgatgta ggtaactgcc cacatcaatg cttcgctttt cagccagaag
      361 ttgaccagcg atgccagcac aatgccgatt aacgccataa acagcatatt gccaaagcca
      421 cttaaatcgc gcttcgtggt gtaaccgtac aggctcattg cgccgaacat cccggcagta
      481 acgacgaaag tactggcgat agaagcagcg gtatagacaa tgaatatact ggaaagcgta
      541 agacccgtca gcgccgaata aagcataaag agcatcgtcg ttacacctgc gctcagcttt
      601 tgaatcatcg ctgataacac aataaccaat gctaattgcg cgatgatcag accgattaaa
      661 aagacacggt tagtgaacaa cagctccatc acggccgcgg aattagccgc ataccaggca
      721 acaaatgcgg tcagcaacaa gccaacggtc atccagccat agacttgcgc catataagtt
      781 tgcaagccag cccggggttg tacgattgaa tcagaacgtg ggaatctgtc catgacgatc
      841 tcctgaagat ataaggaata tcttaaggat actgcaaaat gatgaggctg tgcatcgacg
      901 cagcgtaaac gcatgtactg agcggtgaaa ttgccggacg cagcggtgcc ttatccggct
      961 aacaaaaaac taccagcgtt ttgccgcctg ctgatcgctc tcccgggctt caacccagcg
     1021 gtcgccttcc ggcgtggctt cgcgcttcca gaacggtgcg cgggttttga gataatccat
     1081 aataaactgc ccggcttcaa acgcactgct gcgatgcgca ctggtgacac cgacaaaaac
     1141 gatttcatcg cccggccata attccccgat gcggtgaatc accgtgacgc gccccagcgg
     1201 ccagcggtta cgcgcttcat caacgatttc tgccagtgct ttttcagtca tccccggata
     1261 gtgttcgagg gttaatgctt tgacgctgtc acccaggtta tggttgcgca ccttaccggt
     1321 aaacgtgacc actgcaccgt cttcgtcacg ctcagccagc cacgggtact cttctcctac
     1381 gctgaacggc tgcgggccga caacaatttt ggtttctgcc atcttaacct ccggttaccg
     1441 gcgggaagaa agctacttcg tcgccgtcag tcagcggatg gtcaaaactc accagcgtct
     1501 ggttgacggc agccagcaat ttgccatctt ccagcgccag cgcccagcga tcgctctgcg
     1561 cagccaggtg ctggcgtaac gcttcaacgg ttgggtaatc cgcagccact tcggttgcat
     1621 ctgttcccac caactcgcgc acctgggcga aaaaaagaac tttaatcatc cgcttccacc
     1681 ttaaagtcac ccgacttgcc gccgctcttc gccagcaaac gtaccggacc aatcaccata
     1741 tctttttgca ccgctttgca catgtcataa atggtcagcg ccgccacgga ggccgcggtt
     1801 aacgcttcca tttcgacacc ggttttcccg gtcaggcggc ataaggtttc tatacgcacc
     1861 cgattgtgct ccggctcggc ctgtaaattg acttcaactt tgctgagcat cagcgggtga
     1921 cacagcggga tcagatccca ggtgcgtttt gccgcctgaa taccgacaat acgcgcagtg
     1981 gcaaatacgt cgcctttgtg gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc
     2041 atggtgacaa aggcttccgc ccgcgcttca cgcacggttt ccgctttggc ggagacatcc
     2101 accatgtgcg cttcgccagc ggcgttgata tgggtcagtt gcgacatact tatttcttca
     2161 aatgtggatg gaaattacac ggacgcgtac gggcatccag ctgcggcgcg atgatatttt
     2221 cccatgcggt acggcacgct ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg
     2281 cgacgcccgc taccgcacga gattgcaacg tggaagtgcc aatctcttca aacgacaaca
     2341 tccggaacac ttcaccaaaa ccttcaactt cacggtcgaa caacggcagc aatgcttcgg
     2401 gagcctgatc accttccgtc aggccagtcc cccccgtaat caacaccact tgtacatcgt
     2461 cgctggcgat ccacgcagat acctgagcgc gaatagcgta gcggttttct ttcacaatgg
     2521 ctttatcgac aacgtgatgg cccgcttctt gcgccgaatc gcgcaaatag tgaccggagg
     2581 tatcgtcttc ttcaccgcga cgattagaaa ccgtaagaat agcaatacgg gtcgggataa
     2641 attcagtgct tacctgactc atctgatctc tccttttgac gttttagccg ccaatgtacg
     2701 ataagttttg cgtaataccg gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc
     2761 gcagcgccgc tgaaatacgc gcttccagcg cctgttgctg ggtatcgtct tccagcagat
     2821 cgcgcaggtt aacgccgcct tcaccaaaca ggcagagatg gagtttacca atggaggaaa
     2881 cgcgcaggcg gttgcaagtg gcgcagaagt ctttttcata cggcatgata aggccaatct
     2941 ctccggcgta atctggatgg caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt
     3001 ggtggatcca gccgcgacgc agtagctcgt cacgcagaac ctgaccagag atgtgatgct
     3061 tacggaagag ctcactgccc tcgcccgttt ccatcagttc gatgaaacgc agctggatag
     3121 ggcgatgctg gatccagttc agaaaggtgt cgagctggtg atgattaaca tcacgcatca
     3181 gcacggtatt gactttgacc ttctcaaaac cgacctcaaa tgcagcatcg atccctgcca
     3241 tgacctggtt gaatttatcc tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt
     3301 cgacactgac gttaatgcca gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca
     3361 gacggtaacc attggtggtg accgcaatct ggcggatagc gtcgttttcc cgcacagcgg
     3421 cgatgatatc ggtaaagtcg cggcgtaaag acggctctcc tcctgtcagg cgcacttttt
     3481 cggtgcccag actggcgaag gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc
     3541 ctttattggt gacgccgctc ggtttgtagc catccggcag gcagtaggtg caacgaaagt
     3601 tacacacatc ggtaatcgac aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca
     3661 gttgtgaagc catgtacacc tttccagata cgggaggcga agtcatttct tccttcgccc
     3721 tggtggcaat ctttacgagc attgtcacgg ccaaagcacc gtatcagttg acccaggtgc
     3781 agaggctaga gtgtttagtg gttaagccga tactagcgtg taaatgagat ttttaccatc
     3841 cacactttcg ctatgtaatc atgtatatag cgtcatgatc gtgctatttc gctacagaat
     3901 aagggaaaat ggcagtttta ctttgatata catcatcagg gaggcatagc gtgcatcgtc
     3961 ctttagaggt agaagaaaat agtttgtatc ctcaatattg gcaggttaat tgctgtttcc
     4021 ccgcaatttg cgctactgta gcgcggataa tctgactcca ggaaactata tgcgcaatcg
     4081 tacgctggct gatcttgatc gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt
     4141 tctctcatca ctttcgtctt tgggttctcg tttaacgggt atcgtcacca ccacggataa
     4201 tggtggctcg acggggcgta ttcgccgttc agaaggcggc attgcctggg gcgatatgcg
     4261 caactgcctc aaccaactga taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg
     4321 ttttggcggc aatggcgaac tttccggtca taatctcgga aacttgatgt taaaggcgct
     4381 ggatcacctt agcgtgcggc ctctggaagc catcaattta attcgtaatc tgctgaaagt
     4441 ggatacgcat ttgattccaa tgtcagagca tcccgtcgat ctgatggcga ttgacgatca
     4501 ggggcatgaa gtttacggcg aggtcaatat cgaccagtta actacgccga ttcaagagtt
     4561 attattaacg ccgaatgtac ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga
     4621 tctcatcatt attgggcctg gcagttttta taccagcctg atgccaattc tgctgctgaa
     4681 ggaaatcgcc caggcattac gccgcacgcc agcgccgatg gtctatatcg gcaatctggg
     4741 gcgtgagttg agtttacctg cggctaattt gaagctggaa agcaagctgg caattatgga
     4801 gcagtatgtt ggtaaaaagg tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc
     4861 ggcggtgaaa gagcggattg tgattcagga ggtactggag gccagcgata ttccgtatcg
     4921 tcatgaccgc cagttgttac ataacgcgct ggaaaaggcg ttacaggctt taggttaacg
     4981 gaaatccgaa gaaaaattcc ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg
     5041 atgcggcgtg aacgccttat ccggcctaca aaccgcgcta attcaatgta ttgcagagat
     5101 catgtaggcc tgataagcgt agcacatcag gcaattttgc ttttgtcatc tgtcttcttc
     5161 gttatcctgt cacgaagccg cgataaacag ctcacgcagc tgatgcaact ggtcacgaat
     5221 ttgtgccgct tcttcgaact ccagattctg cgcgtgttgc atcattaacc cttccagctc
     5281 atggattttc tgttgcaacg ctttaggcga catatccatc ggcacattat ccggctcaac
     5341 aatcgggcgc gattttcctc tgcccttcgc tttggttttg gcaatgttct gccccagcgc
     5401 caggatatcg accactttct tgttcaagcc ttgcggcgta attccgtgtt cctcgttgta
     5461 cttctgctgt ttctcacggc gacgttcggt ttcgccaatc gctttcgcca ttgatggggt
     5521 gatcttatcg ccgtagagaa tcgctttacc gttaacgtta cgtgccgcac gaccaatagt
     5581 ctggatcaac gaacgttcgg aacgcaggaa gccttctttg tcagcgtcga ggatcgccac
     5641 cagcgacact tccggcatat ccagaccttc gcgcagtaag ttgatcccta ccagcacgtc
     5701 gaactcaccc agacgcaagt cgcggataat ctccatacgt tcgacggtgt cgatatctga
     5761 atgaagataa cgcacgcgct cgccatgttc ttcgagatat tcggtaagat cttccgccat
     5821 ccgcttggtc agtgtggtga ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc
     5881 cgaaagaaga tcatcaacct gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa
     5941 tccggttgga cgcaccacct gatccaccac atcgccgccg gatttttcca gctcgtaatt
     6001 acccggcgtc gccgaaacat agatggtttg cggcgctaat gcttcgaact cttcaaactt
     6061 aagcggacgg ttatccagcg ctgatggcag gcggaagccg tactccacca gtgtctcttt
     6121 acgcgcccgg tcaccgcgat acatgccgcc aatttgtgga atggtgacgt gagattcatc
     6181 gaccaccagc agcccatcgg caggcaggta atcaaacagc gtcggcggtg gctcacccgg
     6241 tccacgaccg gagaggaagc gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt
     6301 catcatctcc agatcaaact gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt
     6361 gttttccaac agcactttgc gtctggcggc cagttcttct ttgatctcct ccatcgcctg
     6421 tacgatgcgc tcgcgcggtg tgacgtagtg cgttttcggg tagatggtaa aacgtggaat
     6481 agtggaaaca atctgcccgg tcagcgggtc aaataacgac aatcgttcca cttcctcgtc
     6541 aaacagttcc acgcgaagtg caatgtcatc cgattctgcc gggaagatat ctatcacctc
     6601 gccacgaacg cggaaagtac cacgctggaa tgcttgatca ttacgagcgt attgcagctc
     6661 cgccagtcgg cgcagaatcg cgcgctgatc gataatcata ccgaccgtga gatggagcat
     6721 catcttgaga tataaatcag gatcgcccag accataaatc gcggaaacag aggccactac
     6781 aaccacatca cgccgctcca gcatcgcttt ggtggcggat aaacgcatct gctcaatatg
     6841 ttcgttaacc gaggcatctt tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg
     6901 ctgatagtag tcgtagtagg agacgaaata ctccaccgcg ttttccggga agaactcttt
     6961 catttcgcca tacagctggg ccgccagcgt tttgttgggc gcaagtacca tggttgggcg
     7021 ctgaaggtca gcaatgacat tggcaatggt gaaggttttc cctgagccag tcacgccaag
     7081 taacgtctgg tgcgccaggc catcttccag cccctcttcg agacgtcgaa tcgcctctgg
     7141 ctgatcgcca gaaggtttaa aagcggaatt cagtttgaac ggtttactca tgagtcgcta
     7201 cctgaaggag ttgggcgggc aggtatgtaa ttttactcgt cgtacttaat tatgccaaca
     7261 aattatactg gataaaaaaa cagttcatca ccataatatt tctgatacag cgtaaactcc
     7321 gctgtcaatc atgagcaaaa tttactctgt ggcgagataa aactccggcc ttaccgggtt
     7381 atccccaaag caacggcttt tttaacattt gtcaagatga gtgatgacag ttttatggca
     7441 agagatgcct gttcagtgac tgccattgca tttatctaac cagttaaaaa ataaaagata
     7501 tttctttgag ccgtctttaa cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct
     7561 cgtgttttct aactctaata cacatggtta tccacaggaa tagtggataa ctgtctccag
     7621 cccctatccc ccgccgcctg ggcaattccc acattcgccg catgaggcgg aagtaaaatt
     7681 tttttcgtga ttattttaga atttaattgg ttaaattgca gtcaatcgaa gacgcgatct
     7741 cgctcgcaat ttaaccaaat acagaatggc tacaacaagg caaggtttat gtactttccg
     7801 gttgccgcat tttctgcaag ccaggggatc tctcccagca gcggcgcggg aatcatgcgg
     7861 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc gttcgccacc
     7921 caacccgcca gagtcagtcc ggcgtgttgt attgcctgcg cagtcaacat cgcgtgatta
     7981 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
     8041 tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
     8101 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
     8161 ggcctgccct cttgcgcgct gatgatgtgc ggcgaagtgg gttctgcgaa ggtgtaagga
     8221 tttactgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag cgcgtcacta
     8281 ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata acctgccgtc
     8341 cgataaccta ctgccttcgc ggcttgtaaa agtgcacaac tggcgacggt tttccccact
     8401 tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc ccaaaaaaag
     8461 atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca atcgctgcaa
     8521 ttgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag caccgatgcc
     8581 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccat agtgtgatgg gctgaatatg
     8641 atgttgataa tgcacgccgt tcagcgactg ttcgatttca tctggcggta aaaagcgatt
     8701 agcatgcgga cgctcgtcca ccgcctgcca cgcctgatgc agttcgggta acgatccctg
     8761 caccagcgtg gtaaacgcca ccacgcctcc ggggcgcacc acccgataca gctcgcggag
     8821 tgccgtggat aaattaccgc accactgcac cgcgagattg ctccatgcaa gatcgaacgt
     8881 cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatggtctg cagcatcttt
     8941 ctggcgggct tgcacaagca ttggcggtga gagatctaac gccgtcacct gtgcgtgacg
     9001 ctctcgccag tggcggctca tccagcctgg cccacacccc gcgtccagta cgtgggtgta
     9061 tttacgctgt gggagcattg ccagtaagac gtcagcactc cggcgctgta actctgcgtg
     9121 ttgctcatag tgcgcggctg cccgaccaaa tgccgctgca atggcttgtt tattaaccgt
     9181 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgct gcggttagcg
     9241 ttaagcgcag tcgcgcagta ccagcgggta cggttggcgg gcgaatcgcc gtgacccagc
     9301 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccgacgatca
     9361 atggctggat ggcgctgcat gaatcagcaa gcgtaaacgg caaatcctgt actccggcac
     9421 gaaaacgcgc aatgagtgac accagttttt cgcgccgtgc atccccctca tcacggcgaa
     9481 tgaccgccag cgacgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaga
     9541 taaggtggcg ggcgaattgc agcagataat ccgccaccgt actggagcaa agcaccgcag
     9601 ccccactgac gccaaatcct ttgccaaaag tcaccaccag caattctggt tttacctttt
     9661 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcatcatcga
     9721 ccatcaacca gccattgtgc tgttgcgtta cctgctggat ttccgccagt ggcgcgctat
     9781 cgccgtccat gctgaacacg ccttctgtca ccaccagttg ctgccccgga cagggggaag
     9841 caagcagtcg cgccagatga gcgacatcgt tatgagtaaa acggcgaagc tgcgacgggc
     9901 ttaaactggc agcctccagc aaggaggcat ggctaagccg gtcggcaaca atacggtcct
     9961 ctttcgccat catcgcggca atgactgcct gattcgcggc aaaaccagag ataaacagca
    10021 gcgcccgcga atagccaagc cactcggcca gttcttcttc cagcgcctga tgcaccacgc
    10081 tataaccgct gacatgcccg gaaccgccgc tacctacacc aaacttttct gcgccctgct
    10141 gccaggcgcg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    10201 tcagatactg gcgatcatcc gccaccagcc agcgtccggc tccttgcgcc accggataac
    10261 ggcgacgcag ggcatcggca gtacgccgtg catcgagcgc cgcgttgatt ttctcctgcc
    10321 agctcataat gctgccgcgt tgtaatattc gtcggtgtcc ggggtcatca gcgcctgttc
    10381 aagacgttgc tgttgttcgt tatcccctgc cagcacggca gtttgctgcg gatttagccc
    10441 cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg tcagcagttt
    10501 gcaaccgtag aaaatcgagt ttgcgccagc cataaagcac atcgcctgag tctgttcatt
    10561 catctgctcg cgtccggcag aaaggcgcac gtaagaggtc ggcatcatga tccgcgctac
    10621 cgcaatagtg cgaataaaat caaaggcatc gacatcatcg ttatccgcca gtggcgtgcc
    10681 tttcaccttc accagcatgt tgattggtac gctttccggc ggcgtcggca ggtttgccag
    10741 ttgcagcaat aatccggcgc gatcttttac cgtttcgcct aagccaacaa tgccgccaga
    10801 acagactttg atcccggcat cgcgcacttt ttccagcgta tcgaggcgtt cctgataagt
    10861 gcgggtggtg atgatattgc cgtaaaactc cggcgaggtg tccaggttgt ggttgtaata
    10921 atccagcccg gcgttcgcga ggcgctgcgc ctgagattca ctcaacgtgc ccagcgtcat
    10981 acacgcctcc agccccatcg cttttacccc ctgcaccatt tgttccaggt acggcatatc
    11041 gcgttcgtgg ggattcttcc acgccgcgcc catacagaag cgcgtcgatc ctgccgcttt
    11101 cgctttgcgc gccgactcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    11161 ggttttgtag cgtgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    11221 aatcgacagc aacgtgctga cctggacctg acgaggatcg aaatgctggc gatggacctg
    11281 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    11341 ggtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    11401 ggtgtagact tgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    11461 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctccccgc
    11521 tgccggttta tccggtggtg agcgccgaag gttgcgagct gattttgtct gacggcagac
    11581 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat cacccgcagc
    11641 ttaatgcggc gatgaagtcg caaattgatg ccatgtcgca tgtgatgttt ggcggtatca
    11701 cccatgcgcc agccattgag ctgtgccgca aactggtggc gatgacgccg caaccgctgg
    11761 agtgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg aaaatggcgt
    11821 tgcagtactg gcaagccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    11881 atcatggcga tacctttggc gcgatgtcgg tgtgcgatcc ggacaactca atgcacagcc
    11941 tgtggaaagg ctatctgcca gaaaacttgt ttgctcccgc cccacaaagc cgtatggatg
    12001 gcgaatggga tgagcgcgat atggtgggct ttgcccgcct gatggcggcg catcgtcatg
    12061 aaatcgcggc ggtgatcatt gagccgattg tccagggcgc aggcgggatg cgcatgtacc
    12121 atccggaatg gttaaaacga atccgcaaaa tatgcgatcg cgaaggtatc ttgctgattg
    12181 ccgacgagat cgccactgga tttggtcgta ccgggaaact gtttgcctgt gaacatgcag
    12241 aaatcgcgcc ggacattttg tgcctcggta aagccttaac cggcggcaca atgacccttt
    12301 ccgccacact caccacgcgc gaggttgcag aaaccatcag taacggtgaa gccggttgct
    12361 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcggcagca aacgccagcc
    12421 tggcgattct cgaatctggc gactggcagc aacaggtggc ggatattgaa gtacagctgc
    12481 gcgagcaact tgcccccgcc cgtgatgccg aaatggttgc cgatgtgcgc gtactggggg
    12541 ccattggcgt ggtcgaaacc actcatccgg tgaatatggc ggcgctgcaa aaattctttg
    12601 tcgaacaggg tgtctggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    12661 ttattctccc gcaacagttg cagcgtctga ccgcagcggt taaccgcgcg gtacaggatg
    12721 aaacattttt ttgccaataa cgagaagtcc gcgtgagggt ttctggctac actttctgca
    12781 aacaagaaag gagggttcat gaaactcatc agtaacgatc tgcgcgatgg cgataaattg
    12841 ccgcatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    12901 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    12961 gcgccaaccg gctccggctg gtggcactgg gtagttgtta acttacccgc tgatacccgc
    13021 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    13081 cgtaccgact ttggtaaaac cgggtacgat ggcgcagcac cgccgaaagg cgaaactcat
    13141 cgctacattt ttaccgttca cgcgctggat atagaacgta ttgatgtcga tgaaggtgcc
    13201 agcggcgcga tggtcgggtt taacgttcat ttccactctc tggcaagcgc ctcgattact
    13261 gcgatgttta gttaatcact ctgccagatg gcgcaatgcc atctggtatc acttaaaggt
    13321 attaaaaaca actttttgtc tttttacctt cccgtttcgc tcaagttagt ataaaaagct
    13381 gaacgagaaa cagt
//
LOCUS       DAZOYS010000107        13380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOYS010000107 DAZOYS010000000
VERSION     DAZOYS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.107"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHUN9_002706"
     CDS             complement(<1..81)
                     /locus_tag="ACHUN9_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0696062.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUN9_002707"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUN9_002707"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0696063.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUN9_002708"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUN9_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0696064.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUN9_002709"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUN9_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0696065.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUN9_002710"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUN9_002710"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0696066.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUN9_002711"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUN9_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0696067.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHUN9_002712"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHUN9_002712"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0696068.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUN9_002713"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUN9_002713"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0696069.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHUN9_002714"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHUN9_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUN9_002715"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUN9_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0696070.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHUN9_002716"
     CDS             complement(12387..12626)
                     /locus_tag="ACHUN9_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0696071.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUN9_002717"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUN9_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0696072.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHUN9_002718"
     CDS             13003..13290
                     /locus_tag="ACHUN9_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0696073.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3477 a         3305 c         3455 g         3143 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
//
LOCUS       DAZOYS010000108        13350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOYS010000108 DAZOYS010000000
VERSION     DAZOYS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.108"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            87..737
                     /gene="zinT"
                     /locus_tag="ACHUN9_002719"
     CDS             87..737
                     /gene="zinT"
                     /locus_tag="ACHUN9_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0696074.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            1080..1610
                     /locus_tag="ACHUN9_002720"
                     /pseudo
     CDS             1080..1610
                     /locus_tag="ACHUN9_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(2180..2269)
                     /locus_tag="ACHUN9_002721"
     tRNA            complement(2180..2269)
                     /locus_tag="ACHUN9_002721"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2233..2235),aa:Ser,seq:cga)
     gene            2363..3160
                     /gene="mtfA"
                     /locus_tag="ACHUN9_002722"
     CDS             2363..3160
                     /gene="mtfA"
                     /locus_tag="ACHUN9_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0696075.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3261..3336
                     /locus_tag="ACHUN9_002723"
     tRNA            3261..3336
                     /locus_tag="ACHUN9_002723"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3294..3296,aa:Asn,seq:gtt)
     gene            3650..10715
                     /gene="yeeJ"
                     /locus_tag="ACHUN9_002724"
                     /pseudo
     CDS             3650..10715
                     /gene="yeeJ"
                     /locus_tag="ACHUN9_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(10977..12029)
                     /locus_tag="ACHUN9_002725"
     CDS             complement(10977..12029)
                     /locus_tag="ACHUN9_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0696076.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            12344..>13350
                     /gene="shiA"
                     /locus_tag="ACHUN9_002726"
     CDS             12344..>13350
                     /gene="shiA"
                     /locus_tag="ACHUN9_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0696077.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         3727 a         3133 c         3071 g         3419 t
ORIGIN      
        1 tctcatctcc taagagttgt tgcattttgc catatgttac aatataacat tacacatcat
       61 atacaatgac tctggaggaa actgttttgg caattcgtct tcacaaactg gctgttgctt
      121 taggtgtctt tattgttagc gctcctgcct tttcgcatgg tcatcactca cacggcaaac
      181 ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt tgatgatgcc aatgtacaaa
      241 accgaacgct cagtgactgg gatggagtct ggcaatccgt ttatccttta ctgcaaagtg
      301 gcaaacttga ccccgtcttt cagaagaaag cggatgcaga taaaattaaa acatttgctg
      361 aaattaaaga ttattatcac aaaggttatg caacagatat cgagatgatt ggcattgagg
      421 acggcattgt tgaattccat agaaataatg aaacaacatc ctgtaaatat gattacgatg
      481 gatacaaaat actcacctat aaatcaggca agaaaggcgt tcgttactta ttcgaatgta
      541 aagatcctga aagcaaagcc cctaaatata tacaatttag cgatcatata attgcaccac
      601 gaaaatcatc tcattttcac atttttatgg gtaatgattc acagcaattt ttattaaatg
      661 aaatggaaaa ctggccaacg tattatccat atcagttgag tagcgaagaa gtggtcgagg
      721 aaatgatgtc tcattgagta ttctcatgat aacgcctcga tgccgcttta gtaagtcatc
      781 ataactgcca ctggtccatc cacaaacgcc actgaacgca agctagctac agacacgctc
      841 atcactatga cgtgtctgta tattaataag ctaacccgca ttgagttaac caataacgga
      901 ttccatacac aatacggcca acagagaaag atacctgtgc tcacgccatt gcttatattg
      961 gctggttaca atgtgcacta tcaatttttt aaataacaaa ttcttaaaac cacatctgta
     1021 taaggaattt ttaaggttcg tgggatagct tgactgtaaa aattacagga gctacaaaaa
     1081 tgaaccgatt ctcaaaaact caaatttatt tacattggat aacgctgctt ttcgttgcaa
     1141 taacctatgc cgcgatggaa ctccgtggct ggtttcctaa aggtagtagt acttatctgc
     1201 tgatgcgaga aacacattac aatgcgggta tattcgtttg ggtgttaatg ttttcacgcc
     1261 tgattataaa acaccgttat agtgatcctt ctattgtacc accgccacct gcctggcaaa
     1321 tgaaagcggc ttcgctaatg cacatcatgc tttatataac cttccttgca cttcctctgc
     1381 tggggattgc tttgatggct tacagtggaa aatcgtggag tttccttggt ttcaatgtgt
     1441 ctccctttgt taccccaaac agcgaaatta aagcactgat aaaaaatatt cacgaaacct
     1501 aggcaaatat aggttacttt ttaatcgcag ctcacgctgg cgcagcactc tttcatcact
     1561 acattcagaa agataatact ctgttacgaa tgatgcatcg ccgcaaataa gtcgatcccc
     1621 tgacaaggaa atgtgaacca tgtacatcaa cgcgtcataa atgtttacgc aagtgcacaa
     1681 tgcatccttt ctactatcaa caaggaggag cagaaactgt gtaaatcgta caatggtaag
     1741 acttatcaaa aatacaggtt aattttggta tttaaacctt ttttacttgt tagtatgata
     1801 caaaggcttt caaaaaagct gggaaattca acaactcttg gaaattatgg actttgtccc
     1861 gcggctttaa tctgacggcc gcgttccttt tttgactcaa ttattgaccc cttctctacg
     1921 caacttcagt tcccaccacc aactttgcgg cagtttcata ggatgaatgt ctaaaagaat
     1981 aatggtgagc gataagaaaa cgactgaata actgcacatt ttcgctcgaa agcttcctgt
     2041 cagatccata gcgaatcaag tgctgaatgt cacagtatcg aacagaaaac agtgacgatc
     2101 taacccttca agaatattct acaattgttc tgtttaggaa aagcaaggcg ggaagtcagg
     2161 agataagtca ttgataaagt ggcggagaga gggggatttg aacccccggt agagttgccc
     2221 ctactccggt tttcgagacc ggtccgttca gccgctccgg catctctccg ttcagatggt
     2281 tgccatgatg ccaggaaatt tggcatttta acagtccctg tccgtgcaat tttgttcaag
     2341 tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag aatcagcaca
     2401 tcaaactgcc cttccctggc aggaagcact atcgatcccc cttttaacgg gtctgacgga
     2461 acaggaacaa agcaaattag tcactcttgc cgaacgtttt ttacagcaaa agcggcttgt
     2521 tcctttacag ggctttgaac tggattcatt aagaagctgc cggatagcac ttctattttg
     2581 cctacccgtt ctggagttag gactggaatg gctggatggt tttcatgaag tcttaattta
     2641 tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat atcggtctgg tgcataacca
     2701 acgtattgtt cagtcaggtc agagctggca gcaagggcct atcgttttga actggttgga
     2761 tatacaagat tcttttgatg catctggttt taacctgatt attcatgaag tcgctcataa
     2821 gctggacacc cgtaacggcg atcgcgccag cggagttccc tttattccgt tgcgtgaggt
     2881 tgctggctgg gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt
     2941 ggttggcgag aatgcggcga gcattgatgc ttatgcagcc agcgatcctg ctgaatgttt
     3001 tgccgtactt tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc
     3061 attgtggcaa cgtttctgcc aattttatca acaagatcct ttgcagagac tgcatcacgc
     3121 taatgataca gactcgtttt cggcgacgaa tgttcattaa ttaacaactt tgcagattaa
     3181 ttaaccaatt gaaatgactt atgaaattta gtgttgacag acaaggtacc gctaagtaat
     3241 atgtgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     3301 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ctaaaaattc gcttttttag
     3361 cgcaatgtca ctgaccttag ttgaacattg ttttttaacg gatagcgggt ttttaacacc
     3421 ttaagcgccc tcgaccttta tggttgaggg cgttttgcta tgaacgccat caccattttc
     3481 ccctcgatta taaaacttga gttattcagt agtctcccct cttgcaactc acacccaaaa
     3541 ctgcctaacg aaaagttatt aattttcaat cgtattgcta tcagtattta cattttttcg
     3601 ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg aaggtaagca tggctacgaa
     3661 gaagagaagt ggagaagaaa taaatgaccg acaaatatta tgcgggatgg gaattaaact
     3721 acgccgctta actgcgggta tctgtctgat aactcaactt gcgttcccta tggctgcggc
     3781 agcacaaggt gtggtaaacg ccgcaaccca acaaccagtt cctgcacaaa ttgccattgc
     3841 aaatgccaat acggtgccct acacccttgg agcgctggaa tcggcccaaa gcgttgccga
     3901 acgtttcggt atttcggtgg ctgagttacg caaactcaac cagtttcgta cgtttgctcg
     3961 aggttttgat aatgtccgcc agggtgatga actggatgtc ccggcacaag ttagtgaaaa
     4021 aaaattaacc ccgccgccgg gtaatagcag tgacaacctc gagcaacaga tagccagtac
     4081 ttcacagcaa atcgggtctc tgctcgccga agatatgaac agcgagcaag cggcaaatat
     4141 ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg acagactggt taagccgctt
     4201 cggtaccgca agaatcacgc tgggcgtgga tgaagatttt agcctgaaga actcccagtt
     4261 cgattttctc catccgtggt atgaaacgcc tgataatctc tttttcagtc agcatactct
     4321 ccatcgtact gacgagcgta cgcagattaa caacggctta ggttggcgtc atttcactcc
     4381 cacatggatg tcgggcatca acttcttttt cgaccacgat cttagccgtt accactcccg
     4441 cgccggcatt ggcgcggagt actggcgcga ctatctaaaa ttaagcagta acggctattt
     4501 gcgactgacc aactggcgca gcgcacctga actggacaac gattataaag cacgcccggc
     4561 caatggctgg gatgtacgcg cagaaagctg gctacccgcc tggccgcacc ttggcggtaa
     4621 actggtctat gaacagtatt atggcgatga agtggccctg ttcgataaag acgatcggca
     4681 aagtaatcct catgccataa ccgctggact taactatacc cccttcccgc tgatgacctt
     4741 cagcgcggag caacgccagg gtaaacaggg cgaaaatgac acccgttttg ccgtcgattt
     4801 tacctggcaa cctggcagcg caatgcagaa acagcttgac ccgaatgaag tcgctgcacg
     4861 gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc aacaacaata tcgttctgga
     4921 atatcgcaaa aaagaactgg ttcgcctaac cctgacagac cccgtgacag ggaagtcagg
     4981 agaagtgaaa tcactggttt cgtcgctaca aaccaaatat gccctgaaag gctataacgt
     5041 cgaagccacc gcactggaag ctgccggtgg caaagtggtc acaacgggta aagatattct
     5101 ggttaccctg ccggcttacc ggttcaccag tacgccagaa accgataaca cctggccgat
     5161 tgaagtcacc gccgaagatg tcaaaggcaa tttgtcgaat cgtgaacaga gcatggtggt
     5221 cgttcaggca cctacgctaa gccagaaaga ttcctcggta tcgttaagta cccaaacatt
     5281 gaacgcggat tcccattcaa ccgccacact gacttttatt gcgcatgatg cagcaggtaa
     5341 tcctgttgtc gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca tcaccctttc
     5401 tgactggaaa gataatggtg acggaagcta tacccagatc ctgaccacag gagcgatgtc
     5461 tggcacgctg acgctgatgc cacagctgaa tggtgtggat gcggctaaag cccccgccgt
     5521 ggtgaatatc atttctgttt cgtcatcccg aactcactcg tcaattaaga ttgataagga
     5581 ccgttatctc tccggcaatc ctatcgaggt gacggtagaa ctgagagatg aaaatgacaa
     5641 acctgttaag gaacaaaaac agcaactgaa taacgcagtc agcatcgaca acgtgaaacc
     5701 aggagtcact acagactgga aagaaaccgc agatggcgtc tataaggcga cctataccgc
     5761 ctataccaaa ggcagtggac ttactgcgaa gctattggaa tgaagatttg cataccgctg
     5821 gatttatcat cgacgccaac ccgcagtcag cgaaaattgc gacattatct gccagcaata
     5881 atggtgtact cgccaatgag aatgcagcaa acaccgtctc ggtcaatgtc gctgatgaag
     5941 gaagcaaccc aatcaatgat cataccgtca cgtttgcggt attaagcgga tcggcaactt
     6001 ccttcaacaa tcaaaacacc gcaaaaacgg atgttaatgg tctggcgact tttgatctga
     6061 aaagtagtaa gcaggaagac aacacggttg aagtcaccct tgaaaatggc gtgaaacaaa
     6121 cgttaatcgt cagttttgtc ggcgactcga gtaccgcgca ggttgatctg cagaagtcga
     6181 aaaatgaagt ggttgctgac ggcaatgaca gcgccacaat gaccgcgacc gtccgggatg
     6241 caaaaggcaa cctgctcaat gacgtcatgg tcactttcaa tgttaattca gcagaggcga
     6301 aactgagcca aaccgaagtg aatagccacg acgggatcgc cacagctacg ctgaccagtt
     6361 tgaaaaatgg tgattatagg gttacggcct ctgtgagctc tggttcccag gctaatcaac
     6421 aggtgaattt tatcggtgat caaagtactg ctgccctgac cctcagtgtg ccttcaggtg
     6481 atatcaccgt caccaacaca gctccgcaac atatgactgc aaccttgcag gataaaaatg
     6541 gcaacccact aaaagataaa gaaatcacct tctctgtgcc aaacgacgtc gcaagtaagt
     6601 tctcgattag caacggagga aaaggcatga cggatagtaa cggggttgca atcgcctccc
     6661 tgaccggcac gttagcgggc acgcatatga tcatggctcg tctggctaac agcaatgtca
     6721 gcgatgcaca gccaatgacg tttgtggcgg ataaagacag agcggttgtc gttttgcaaa
     6781 catcgaaagc ggaaatcatt gggaatggcg tggatgagac aactctgaca gcaacagtga
     6841 aagatccgtc gaatcatccg gtggcgggga taacggtaaa cttcaccatg ccacaggacg
     6901 ttgcggcaaa ctttaccctt gaaaataacg gtattgccat cactcaggcc aatggggaag
     6961 cgcatgtcac gctgaaaggt aaaaaagcgg gcacgcatac ggttaccgca acgctgggta
     7021 ataacaatac cagtgattcg cagccggtaa catttgtggc ggacaaagcc tcggctcagg
     7081 ttgtcctgca gatatcaaaa gatgagatca caggtaatgg cgtcgatagc gcaacgctaa
     7141 ctgcaacggt taaagatcag ttcgacaatg aggtgaataa tcttccggta acattcagct
     7201 cagcctcttc aggactcacc ctgaccccgg gagtaagtaa taccaatgag tctggcatcg
     7261 cgcaggccac tctcgcaggc gttgcctttg gtgagcagac ggtcactgca tcactggcta
     7321 ataatggtgc cagcgacaac aaaactgtgc attttattgg cgacacagcg gcggcaaaaa
     7381 ttatcgagtt gacgcctgtc ccagacagca taatcgccgg taccccgcag aacagctccg
     7441 gcagcgccat caccgccaca gtcgttgata ataatggctt tccggtgaaa ggtgtgactg
     7501 tgaacttcac cagcagaaca aactctgccg aaatgacgaa tggcggccaa gccgtaacga
     7561 acgaacaggg taaggctacc gtcacttata ccaatacccg ctcctcgata gaatcaggag
     7621 cgagaccgga taccgttgag gccagtctgg aaaatggtag ctccacgctt agcacatcaa
     7681 ttaatgtcaa cgctgatgcg tctacggcac atctcacctg gctacaggca ctttttgata
     7741 cagtctccgc aggcgagaca accagtctgt atattgaggt gaaggataat tacggcaacg
     7801 gtgtccccca gcatcaggtg accctcagcg tttcaccaag tgaaggcgtg acccccagta
     7861 ataacgctat atatactacc aaccacgacg gcaattttta cgcaagcttt accgctacaa
     7921 aagccggggt ttatcaagtg acggcaaccc tcgaaaatgg cgattcgatg caacaaacag
     7981 tgacctatgt gccgaacgtc acgaatgccg aaatcacgct ggcaacctcg aaggatccgg
     8041 tgattgccga caataacgat ctcacgacac taacagcaac agtcgctgat acagagggca
     8101 atgcgatagc caacactgaa gtaacattta ctctgccgga agatgttaag gcgaacttca
     8161 cgctgagcga tggcggtaaa gcgattactg atgctgaagg caaagcgaaa gtcacgctga
     8221 aaggtacaaa agcaggcgct catactgtta cagcatcgat aactggcggt aagagtgagc
     8281 agttggtggt gaactttact gcggatacgc tcactgcgca ggttaatctt aacgttaccg
     8341 aggacaattt tatcgctaat aacgtcggga tgaccaggct gcaggcaaca gtgactgatg
     8401 gaaacggcaa cccgttagcc aatgaggcgg tgacattcac gctaccggca gatgtgagcg
     8461 caagctttac tctcggacaa ggcggttccg ccattactga catcaacggc aaggctgaag
     8521 ttacactgag cggtacaaaa tccggcacct accccgtgac agttagcgtg aacaattatg
     8581 gtgtcagtga tacgaaacag gtgactttga ttgccgatgc tggtaccgca acactagcct
     8641 ccttaacctc tgtatactca ttcgtcgtca gcacgaccga gggcgcgacc atgactgcaa
     8701 gcgtcactga cgctaacggc aacccggtag aaggcataaa agttaatttc cgcggaacct
     8761 ccgtcacgct cagcagcacc agcgttgaaa cggatgatcg gggtttcact gaaattcttg
     8821 tgacaagcac cgaagtcgga ctgaaaacag tttcagcctc tctggcagat aaacctactg
     8881 aagtcatatc gcgattactg aatgcaaaag cagatattaa ttctgcaacg attaccagtc
     8941 tggagatacc tgaaggtcag gtcatggtcg cacaagacgt agcagttaaa gctcacgtca
     9001 acgaccagtt tggcaacccg attcttaatg aatctgtaac attcagtgca gagccaccag
     9061 agcacatgac catcagccaa aatattgtct ctactgatac gcatggtata gccgaggtct
     9121 ccatgacgcc cgaaagaaac ggttcgtata tggtgaaagc atccctggcg aatggagcct
     9181 cacttgagaa acaactggag gctattgatg aaaaactgac actctcggcg tccagcccgc
     9241 ttatcggtgt caattcccca acaggtgcaa ctctgacggc aacgctaact tctgcaaatg
     9301 gcactccagt ggagggtcag gtcatcaact ttagcgtaac gccagaaggt gcgacgttaa
     9361 gtggcggaaa agtgagaact aactcttctg gtcaggctcc agtcgttctg accagcaata
     9421 aagtcggtac atatacggtg actgcatcgt tccataacgg cgtaacaata cagacacaga
     9481 caaccgtgaa agtcactggc aactcaagca ccgcccatgt tactagcttt atcgctgatc
     9541 catcgactat cgccgccacc aacagtgatt taagtacctt aaaggcaacg gttgaggatg
     9601 gcagtggtaa cctgatcgaa ggtctcactg tgtactttgc cttaaaaagc ggctctgcca
     9661 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca agcgtgaaag
     9721 gagcgatgac aggtagcgtc acggtaagcg cagtcacgac cgctggtgga atgcaaacag
     9781 tagatataac gctggtggca ggcccagcag acgcctcaaa gtcagtcctt aagaacaatc
     9841 ggtcatcatt gaaaggagac tttaccgata gtgctgagct acatcttgtt ctgcacgata
     9901 tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt tgtacagtca ggtaccaacg
     9961 tgccctatgt gcaagttagt gcaattgact acagtaaaaa tttctcaggc gagtacaaag
    10021 ccactgttac aggaggcgga gagggtatca caacgctgat ccctgtattg aatggtgttc
    10081 atcaagctgg tctgagtacc acaatacaat tcactcgcgc agaagacaaa ataatgagcg
    10141 gtacagtatc agtcaatggt actgacctac cgacaactac attcccttcg caggggttca
    10201 ccggggcgta ttatcagttg aataatgaca actttgcccc aggaaaaacg gcggctgatt
    10261 atgagttttc aagctctgcc tcctgggtcg atgttgatgc taccggtaaa gtgacattta
    10321 aaaatgtcgg cagcaattcg gaaaggatta cggcgacacc aaaatcagga ggccctagct
    10381 atgtatacga aatccgtgtg aagagttggt gggtgaacgc cggcgaggct ttcatgatat
    10441 acagccttgc tgaaaatttt tgcagcagca atggctacac gctccccaga gcaaactatt
    10501 taaaccacag tagttcccga ggcatcgggt cactgtacag tgaatgggga gatatggggc
    10561 attacacgac tgaagctggt tttcaatcaa atatgtattg gtcatctagt cccgcaaact
    10621 caagcgaaca atacgtagtt tccctggcaa caggtgatca aagcgtattt gaaaagcttg
    10681 ggtttgctta tgcgacatgt tataaaaacc tctgattttc cgttataatt tcttaaagag
    10741 tgctccattc ggagctctct tttttacatt ccctctatag agcttgccct tctctcacag
    10801 aaaatgaata aataaaaatg cggcaccgcc agaattgcgt tcgatgtgac tatgatcgta
    10861 tccggtgagt actgctttga ttcaaaggct ggttttctgt gatgattgac cagcctcaga
    10921 gtattgatga caccaccgtt cataacagat cggcattact atgcataagt actgtctcat
    10981 attctcgcca aaacactttt taatgccttt gcgaattcat ccagacttat attgtcaatt
    11041 ttttgcccaa cgacgactga ctccgagaca tcggccattg cgggccatcg cgttttgtaa
    11101 tcagcataaa ttgcaacaac aggggtatta taggcacttg ccatatgtaa tattgaggtg
    11161 tcaggagtta ttgcgatata agcatctttt atgattgctg ctgaacgttt aatcgagggt
    11221 ggtaaggata aacggtagac attattataa caatccacta atgccctggc tttatcttca
    11281 cctttgggtc catgaacgat cactattggt atatctgttt cactttgaat ttttgctatt
    11341 agattttctg caatcgataa tgaaaatgta cgttcctgcg agctaccttc taaattgaac
    11401 gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct catcgagtac atcctcactt
    11461 agtggcaatt catatattgg cctgactgct ggaaaacccg cctcacgcat cataaatgcc
    11521 catgtcatag gtacgggagc ccggagtttc tgatccatac gggacgcgtt cttgcacaaa
    11581 ggggagtagc aattcatggt tataccaaca acctgaaaat tcgtttttgc tttcaactga
    11641 ctgataaata acatcgtttt caggttcttt ttacgcatcc cctcaataca aagatccggc
    11701 gtaccgtatt gctgtgttat gttctttgct aaatctttta tttcttttaa tgttgcgtga
    11761 tcctgcatag tcattgtgac taatgttaat ttagtctgtt caagtttaag cgcattaaag
    11821 acttctaaat taattgtcga cgttacaatt aaaagatgct taattttatg caattcaagc
    11881 gcccgaataa caggaaagat ggccatagca tcgccaatct gatcgggaat atggatgaca
    11941 acaaagtctg ttttttcaat attgaaatta taagctttat aatcgtagta actaaatgca
    12001 atacgtctca acaatgatgc taaaaacata cctaacctcg cctccctact ggttataatg
    12061 caatgcagtc tatcagactc atcagggtgc cattttgtgc atatgcggac ttttatgttt
    12121 catatctcta acctgtgggt cctctgctta atccttaaac aacaccagca actcctgcgc
    12181 tttcatcttc catcgaattt ttcatgttgc cgctaatcag ccataaaatc atttgcagat
    12241 gcgctctgtc gaggtagtct cataaggttc gtttatagat cgacggcaat gtgagttacc
    12301 ttttccatac taattataaa aagacagtac aaacaggatc attatggact ccacgctcat
    12361 ctccactcgt cccgatgaag ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg
    12421 cagcttcgct ggtgccgtcg tcgactggta tgatttttta ctctatggca tcaccgccgc
    12481 actggtgttt aatcgcgagt ttttcccgca agtaagcccg gcgatgggaa cgctcgccgc
    12541 atttgctacc tttggcgtcg gattcctttt ccgtccgctc ggcggtgtca ttttcggtca
    12601 ctttggcgac cgactgggac gtaagcgcat gttaatgctg accgtctgga tgatgggcat
    12661 cgcgacagcc ttgattggta ttcttccttc attctcgacc attgggtggt gggcacctat
    12721 tttgctggtg acactgcgtg tcattcaggg atttgccgtc ggcggcgaat ggggaggcgc
    12781 ggcgttgctt tccgttgaaa gtgcaccgaa aaataaaaaa gccttttaca gtagcggtgt
    12841 acaagttggc tacggtgtag gtttactgct ttcaaccgga ctggtttcat tgatcagtat
    12901 gatgacgact gacgaacagt ttttaagctg gggctggcgc attcctttcc tgtttagcat
    12961 cgtactggta ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga
    13021 acaacagcaa cattatcaag ctgccgcgaa aaaacgcatc ccggttatcg aagcgctgtt
    13081 acgacatccc ggtgctttcc tgaagattat tgcgctacga ctgtgcgaat tgctgacgat
    13141 gtacatcgtt actgcctttg cacttaatta ttcaacccag aatatggggc taccgcgcga
    13201 acttttcctt aatattggtt tgctggtagg tggattaagc tgcctgacaa ttccctgttt
    13261 tgcctggctt gccgatcgtt ttggtcgccg tagggtttat atcacaggtg cgttaatcgg
    13321 aacgttgagc gcatttcctt tctttatggc
//
LOCUS       DAZOYS010000109        13302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOYS010000109 DAZOYS010000000
VERSION     DAZOYS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.109"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUN9_002727"
     CDS             complement(<1..696)
                     /locus_tag="ACHUN9_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0696078.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUN9_002728"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUN9_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0696079.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUN9_002729"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUN9_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0696080.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUN9_002730"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUN9_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0696081.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUN9_002731"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUN9_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0696082.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUN9_002732"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUN9_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0696083.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUN9_002733"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUN9_002733"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0696084.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUN9_002734"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUN9_002734"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0696085.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUN9_002735"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUN9_002735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0696086.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUN9_002736"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUN9_002736"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0696087.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUN9_002737"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUN9_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0696088.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUN9_002738"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUN9_002738"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0696089.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUN9_002739"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUN9_002739"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0696090.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUN9_002740"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUN9_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0696091.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUN9_002741"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUN9_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0696092.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="ACHUN9_002742"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="ACHUN9_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..>13302)
                     /locus_tag="ACHUN9_002743"
     CDS             complement(12845..>13302)
                     /locus_tag="ACHUN9_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0696093.1"
                     /translation="HVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYL
                     LMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWR
                     GMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         3376 a         3294 c         3436 g         3196 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gg
//
LOCUS       DAZOYS010000110        13261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOYS010000110 DAZOYS010000000
VERSION     DAZOYS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.110"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUN9_002744"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUN9_002744"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0696094.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUN9_002745"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUN9_002745"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0696095.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUN9_002746"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUN9_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0696096.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUN9_002747"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUN9_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0696097.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUN9_002748"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUN9_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0696098.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUN9_002749"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUN9_002749"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0696099.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUN9_002750"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUN9_002750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0696100.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUN9_002751"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUN9_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0696101.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUN9_002752"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUN9_002752"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0696102.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUN9_002753"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUN9_002753"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0696103.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUN9_002754"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUN9_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0696104.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13261
                     /locus_tag="ACHUN9_002755"
     CDS             13056..>13261
                     /locus_tag="ACHUN9_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0696105.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3367 a         3372 c         3487 g         3035 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 t
//
LOCUS       DAZOYS010000111        13127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOYS010000111 DAZOYS010000000
VERSION     DAZOYS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.111"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            78..1298
                     /locus_tag="ACHUN9_002756"
     CDS             78..1298
                     /locus_tag="ACHUN9_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0696106.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1271..1900
                     /locus_tag="ACHUN9_002757"
     CDS             1271..1900
                     /locus_tag="ACHUN9_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0696107.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1901..3061)
                     /gene="ybdL"
                     /locus_tag="ACHUN9_002758"
     CDS             complement(1901..3061)
                     /gene="ybdL"
                     /locus_tag="ACHUN9_002758"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0696108.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3170..4258
                     /gene="hcxA"
                     /locus_tag="ACHUN9_002759"
     CDS             3170..4258
                     /gene="hcxA"
                     /locus_tag="ACHUN9_002759"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0696109.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4268..4465)
                     /locus_tag="ACHUN9_002760"
     CDS             complement(4268..4465)
                     /locus_tag="ACHUN9_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0696110.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4647..6752)
                     /gene="cstA"
                     /locus_tag="ACHUN9_002761"
     CDS             complement(4647..6752)
                     /gene="cstA"
                     /locus_tag="ACHUN9_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0696111.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6933..7346)
                     /gene="entH"
                     /locus_tag="ACHUN9_002762"
     CDS             complement(6933..7346)
                     /gene="entH"
                     /locus_tag="ACHUN9_002762"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0696112.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7349..8095)
                     /gene="entA"
                     /locus_tag="ACHUN9_002763"
     CDS             complement(7349..8095)
                     /gene="entA"
                     /locus_tag="ACHUN9_002763"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0696113.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8095..8952)
                     /gene="entB"
                     /locus_tag="ACHUN9_002764"
     CDS             complement(8095..8952)
                     /gene="entB"
                     /locus_tag="ACHUN9_002764"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0696114.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8966..10576)
                     /gene="entE"
                     /locus_tag="ACHUN9_002765"
     CDS             complement(8966..10576)
                     /gene="entE"
                     /locus_tag="ACHUN9_002765"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0696115.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10586..11761)
                     /gene="entC"
                     /locus_tag="ACHUN9_002766"
     CDS             complement(10586..11761)
                     /gene="entC"
                     /locus_tag="ACHUN9_002766"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0696116.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12136..13101
                     /gene="fepB"
                     /locus_tag="ACHUN9_002767"
     CDS             12136..13101
                     /gene="fepB"
                     /locus_tag="ACHUN9_002767"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0696117.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
BASE COUNT         3180 a         3743 c         3336 g         2868 t
ORIGIN      
        1 ctgctttaca gtgtttactt tataattaac agtcttatct cactgttcac cctgtaacgt
       61 tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag
      121 ctgcacgaga gcgtattacc tggacactca acaccctacc ccgcgtttgt gtctcatttt
      181 ctggcggcaa aaactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg
      241 gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca attctcttgc accattaact
      301 atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga agagttttac tgggttgcgc
      361 tcccgcttac gacgcaaaat tccctttcac aataccaacc cgaatggcag tgctgggaac
      421 ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct gactttttct
      481 gcttttacca gccaggcatg accttcgaac aatttgtacg tgagtttgcc gaatggtttt
      541 cacaaaaacg tccggtggcg atgatgatcg gcatccgtgc ggatgagtcc tacaaccgtt
      601 ttgtcgccat cgccagttta aataaacaac gttttgccga cgataaacca tggactacgg
      661 ccgcaccagg cggtcatagc tggtacattt accccattta cgactggaaa gtggctgata
      721 tctggacctg gtatgcaaat catcaaaatc tctgcaaccc actgtataac ctaatgtatc
      781 aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg gagcaacgac
      841 aaggattatg gctctatcac gttatcgaac cggatcgctg ggctgctatg tgcgcacgag
      901 tcagcggggt aaaaagtggc ggcatttacg ccggacatga taatcatttc tatgggcatc
      961 ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg ctgctcaata
     1021 gcatgccgga aaaaacagct gagcattacc gcaataaaat cgccatttat ttgcactggt
     1081 atcagaaaaa aggcatcgag gttccacaaa cccagcaagg ggacattggc gcgaaagata
     1141 tcccctcctg gcggcggata tgcaaagttt tactcaataa cgattactgg tgtcgggcat
     1201 tatcatttag tcctacgaaa gcgaagaact atcagcgtta taacgaacgg ataaaaggaa
     1261 aacgtcagga atgggggata ctatgcaaca acgattaacg caggatttaa cgcaatttct
     1321 cgccagcttg ccggaagatg atcgcatcaa agcgattaat gaaatccgca tggcgatcca
     1381 tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta aaaacagcca
     1441 acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca
     1501 aaaatccatt gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa
     1561 tgccatggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt
     1621 aaaattacgc ttaaaaggtt atttgccggt gatctgtctg gaaggtacgc gtaatcaacg
     1681 catcgccgcg actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc
     1741 ggaaatcgtc cgtgaactta gccagttggg atgggacgat aataaaattg gcaaagagct
     1801 gggaatggac agcgacgaag tgctgcgcct gaagcaaatt aacggcctgc aagagctgtt
     1861 tgccgaccgt caatattccc gcgcttggac ggttaaataa ctaaagctgg cgcaggcgtt
     1921 cagctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg
     1981 ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca
     2041 gccactggca aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa
     2101 agtatgtacc ttcacacggt aaaatctcca gccggctttc atttaaggca tttaccagaa
     2161 tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta
     2221 gcatatcagc aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta
     2281 ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg
     2341 tcatatgata ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcgaat
     2401 gcgccagcac actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc
     2461 taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct
     2521 gccagacagt tgcactggag ttatgcggag tgttgaggat caccagtctg gtgcgctcgc
     2581 ttaataatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac
     2641 gcttcactat tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa
     2701 aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg
     2761 tcgcccctgc cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt
     2821 ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt
     2881 ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat
     2941 caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg
     3001 tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca
     3061 tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ctagtatttg
     3121 gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa
     3181 tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca
     3241 cctgaacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg
     3301 cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca
     3361 tattttgttt cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg
     3421 tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc
     3481 cctcgcccgc cgtctcggtc tgccatttgt tgccgttccg acgatcgccg ctacctgcgc
     3541 cgcctggaca ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat
     3601 tttcgacgac gccaatttta tggtgctggt ggaaccagag attatcctca acgcgccgca
     3661 acaatatctg ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct
     3721 ggctccgcaa ccagaaacgt tgccgctaac cgtgcgactg gggatcaata atgcgcaggc
     3781 cattcgcgac gtattgttaa acagtagcga acaggcgctg agcgatcagc aaaatcaaca
     3841 gttaacgcaa tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg
     3901 tggtctgggc gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac
     3961 tgtcctgccg caaaccgaga aatttctcca cggcaccaaa gtcgcttatg gcattctggt
     4021 acaaagcgcc ttgctaggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg
     4081 ttttcatctg ccgaccacgc tggcggagct ggaaatggat atcaataatc atgcggagat
     4141 cgacaaagtg attacccaca ccctgcgtcc ggtggaatac attcattacc tgccagtcac
     4201 gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg
     4261 tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac
     4321 ggaaaaactc ttcgtaggtc atcggcgttt gatcgggatg gttaacccgc atatgttcga
     4381 catagttgtc gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc
     4441 cggcttttgc cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc
     4501 cggatgcggc gtgaacgcct tatccggcct acaaatgcta tcaacgcgct cggtcagtcc
     4561 cctcgcccca tcggggagag ggttagggtg aggaggaacc ctcggcacta tgccaacatt
     4621 cgccaacatc ccccctcact ctgactttag tgtgcgcctt ttgcctgcgc cacgatctcc
     4681 tcgacatttt ccggcattgg ctcatacggc gtttctttcg ccgttggctt cggatctttc
     4741 aatgccgcca gtgccgactt aatcgagaac agcgccagaa ccaccacgac caccataaag
     4801 aagatggtta acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca
     4861 gtatactgcg acggaatatt gccgctgtcg atcattgcct ggaacttatt agcaatggcc
     4921 aggaagccga ctttcgcatc cgggctaaac gctttctgcc agcctgcggt cagggtacaa
     4981 atcagcagcc aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg
     5041 aacaacacca cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca
     5101 aacagcggcc acagagtgtt aatgccgccc agcggatcga ccacgccctg atggaggaag
     5161 tagccccacg ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc
     5221 cgcttcaggc caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc
     5281 gtacccgcat ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac
     5341 cagaacgcca catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc
     5401 gccagcgtcg gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca
     5461 atctggttga gcgtatccgg agtaatcgca aagccccagc tactcaccac ctgcgcggca
     5521 gaagcgacca catccgccgt ccctgccgga gccagcaccg ccatcgggct gttcatggcg
     5581 aagtacacgc ccggatcgat gatacaggcg gaaaccagtg ccataatcgc cacgaaggat
     5641 tccattaaca ttccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc
     5701 ggcgtggtgc cggaagagat cagcgcatgg aagcctgaca ccgcgccaca ggcgatggtg
     5761 ataaacagga acgggaacag attaccggtc cataccgggc cagtgccatc gacaaatttg
     5821 gtcagcgcag gcatggtcag cgtcggacgc ataatcaaaa tgcctaccgc cagaccaacg
     5881 atagtcccga ttttcaggaa ggtagagagg tagtcacgcg gggccagcag taaccacacc
     5941 ggcagcaccg ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta
     6001 aagtcaaagt acggtgccca ggtcgggctt tctgccaccc agccgccaga gataatggca
     6061 aaaataagga acaccaggcc aatcactgac acttcgccaa tacgccccgg acgcagatag
     6121 cgcaggtaga ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc
     6181 cacgggctat gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc
     6241 atgatcataa agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg
     6301 accagctcac ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc
     6361 tgcaccgcac cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc
     6421 atttgcgctg ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg
     6481 tgaccgaaca gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc
     6541 gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa
     6601 aaacggtaag cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc
     6661 tgttccccac gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact
     6721 gtccagacga ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga
     6781 gatgttacat tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa
     6841 atcggagttg aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag
     6901 ttttcctgca atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga
     6961 caagtgcagc aacgccgccc ctgttcatcg aaaacgatga tttcccagct ctgattttgc
     7021 cgcccaagat gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga
     7081 tgggtggcgt taagttccgt acccaccaca cactgcccgt cacgggtcat cataaatccg
     7141 gccatcgatc ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac
     7201 ggctgatgag tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagacgg
     7261 gtatacacaa ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg
     7321 agcgttaaat ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca
     7381 caatatcctg tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg
     7441 cgatctcttg tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa
     7501 agccgcgaat acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg
     7561 tgtcggtgga gccaggcgaa accacattac agcgcacgcc gctaaccgcc agttccagcc
     7621 cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa
     7681 tacgcggcgt gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc
     7741 gaaactggtt catggtttgc tgaaacaggt taaacgcacc gccgacgtta accgcaaaag
     7801 tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg
     7861 cattgaccag cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcga
     7921 cctgcgcagc atcggcaaca tccatcactt cggtcgcaaa gggatattgc tcctgagcga
     7981 acgcttgatc aaaaccggta actttcgctc ccgcttcaac aaacgccagc gccgtggcgt
     8041 agccgatacc tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt
     8101 cacctcgcgg gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac
     8161 aaagtcgata tcgccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga
     8221 atccagaccg tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa
     8281 cggcaggata acctcacgca gcgccgcttt cctggcgggg acaggtgctg gcagtaatcc
     8341 ttcagtcatc accacccggc cagaccgccc ggccacatat ttcagcgaca tcaaatgctc
     8401 gtcacggctg aaatcggcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa
     8461 tgcgtcggtt gcggtggtca tacagccaat gtgggcatat accccggtaa taatcagctg
     8521 gttacgtcca ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg
     8581 ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg
     8641 ttccggcgaa cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc
     8701 gctctgctct ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc
     8761 gcgcagcgca gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa
     8821 gctgacaaaa tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc
     8881 ccagtcaact ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt
     8941 tggaatagcc atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta
     9001 attgtttttt atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc
     9061 gatccggtaa tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg
     9121 cgcgcagcgg ctcttttacc accagataag cacagctttt ttcgcccatc agctcatctt
     9181 ccatgctcac cagtgcggca tagatcaccg ccggatggcg cagcagcagg ttttcgatct
     9241 cttcggcagc gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg
     9301 tgatgtaacc ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat
     9361 caaaggcact ggcattgtgc tgcggacttt tgtaataacc gcggaaggtg tacggcccgc
     9421 gcgtcatcag gcgtccaact tccccttgcg gcagtggatt tccttcagca tcagcaaccc
     9481 atacttcatc gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat
     9541 catcaagtcg ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc
     9601 agccaatctc agcggaaata cgcgccgcaa gggtggcaga aagacgtgcg ccgccgacct
     9661 gtaacagttt cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc
     9721 acaggctgac tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg
     9781 ggaagcaaag cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga
     9841 cgcccagcga tcctggcgaa ctcatagcgt agttatgagc cgccgggatc gcgcacaggt
     9901 agcgtgtctg ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg tagtagtagt
     9961 cgttatgagt gcgcgggatc agcttcggtg taccggtggt gccgccggaa agctggaaat
    10021 aggccacttc atcagcaggt gatggcgtag cagtaaaacc gtctgccgga tggttaatcg
    10081 catcctgcaa gttatgctca ccgctgtcgt tgagcagttg caccacgcga atggaggaat
    10141 gttctgtgac gaaagtattg aggaaatcat ccccgctaaa cagcgcatgt tggcgatcgg
    10201 caatcagtaa cgccggttca atctggctgg cataggcgtt cagttcacta cgctgatggc
    10261 tgaacaacgc cagcaccggc gcaacgccca gtttcagcag tgcgaaaaag gtaatgtaaa
    10321 gttcagcgac gttacccagt tgtaccagcg cggtttcacc gggtttaatg ccctgacggc
    10381 gtaaactaca cgcgaggtta tccgccgcct gattcagctc ccggtaactc aactgtcgct
    10441 cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagcg
    10501 gcaaatcctg ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg
    10561 tgaatggaat gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa
    10621 gtttgacgcc tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc
    10681 cggcaaacag acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt
    10741 cgccgttacc ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcaaacg
    10801 gctccagttc agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg
    10861 tcggatgcag cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa
    10921 agggagttgc gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt
    10981 gtaactcact actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc agttcatgtt
    11041 catggcgatc tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat
    11101 ccggctgacg acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac
    11161 gtagcagcag ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga
    11221 agttgtaact aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg
    11281 cggcgtcagt ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg
    11341 cggtaagtgc ggcggcgcgg gcaaccatct gttcaaacgt ggtttgttca ggaattgcct
    11401 ggcgttccac cacgttcagc gactggctgc gggtaaaaca gtgggctgag gtctgttttt
    11461 cctgacggga gaacgactgc caggattcgg gaatatacag cgacgaaggc tgacgtggat
    11521 cgaagggaat cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa
    11581 acagcgcggc gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt
    11641 catcgaagcg ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa
    11701 agcgattggg cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca
    11761 tatcatcctc cacaaaatga taaaggcttt aataatgatt atcatttata ttttcggtcg
    11821 ctaacctaaa agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg
    11881 ggttgcatct ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag
    11941 cgcaatgtga tgtcctgcgc cgttttgccc cctctccctt ccagggtgag ggctggggtg
    12001 agggttaatg ttcgcaccag tgctggctgt tcccctcacc ctaaccctct ccccaaaggg
    12061 gcgaggggac ggattgtgcg ctttgtcgaa tttgtcatta cgcccttaac cttattaata
    12121 acaggaagct gacttgtgag actcgccccg ctctaccgca acgcccttct attaacagga
    12181 cttttgcttt caggaatagc cgtagttcag gccgccgact ggccgcgtca gattactgac
    12241 agccggggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc
    12301 ctgaccggat cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgccg
    12361 aataaccgcg tcgcagatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
    12421 cgcaaactgc aacggctcta tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg
    12481 ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcgct gtatgatcag
    12541 ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg gcagtcgctg
    12601 ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcacag
    12661 tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact
    12721 gccattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
    12781 gggcagatgc tggaacaact cggctttacg ctggcgaagt tgcccgcagg gttaaacgcc
    12841 agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggggaaaa tctggctgct
    12901 gggttaaacg gtgaatcgct attcctgttc gccggtgatc agaaagacgc cgatgcgatt
    12961 tatgccaatc cactgctcgc gcacctgcct gcagtacaaa acaagcaggt ttatacgctg
    13021 ggaaccgaga cgttccgcct ggattactac agcgccatgc aagtgctgga cagacttaat
    13081 tcactgttaa aaaaagattg ataaaacata caattagagc actaata
//
LOCUS       DAZOYS010000112        13033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOYS010000112 DAZOYS010000000
VERSION     DAZOYS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.112"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUN9_002768"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUN9_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0696118.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUN9_002769"
     CDS             1598..4654
                     /locus_tag="ACHUN9_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0696119.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUN9_002770"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUN9_002770"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0696120.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUN9_002771"
     CDS             5161..5349
                     /locus_tag="ACHUN9_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0696121.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUN9_002772"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUN9_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0696122.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUN9_002773"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUN9_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0696123.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUN9_002774"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUN9_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0696124.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUN9_002775"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUN9_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0696125.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUN9_002776"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUN9_002776"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0696126.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUN9_002777"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUN9_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0696127.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUN9_002778"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUN9_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0696128.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUN9_002779"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUN9_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0696129.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3268 a         3217 c         3504 g         3044 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gct
//
LOCUS       DAZOYS010000113        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOYS010000113 DAZOYS010000000
VERSION     DAZOYS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.113"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUN9_002780"
     CDS             <1..191
                     /locus_tag="ACHUN9_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696130.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUN9_002781"
     CDS             191..1393
                     /locus_tag="ACHUN9_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0696131.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUN9_002782"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUN9_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0696132.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUN9_002783"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUN9_002783"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0696133.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUN9_002784"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUN9_002784"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0696134.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUN9_002785"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUN9_002785"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0696135.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUN9_002786"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUN9_002786"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0696136.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUN9_002787"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUN9_002787"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0696137.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUN9_002788"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUN9_002788"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0696138.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZOYS010000114        12947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOYS010000114 DAZOYS010000000
VERSION     DAZOYS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.114"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            22..339
                     /locus_tag="ACHUN9_002789"
     CDS             22..339
                     /locus_tag="ACHUN9_002789"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0696139.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            408..737
                     /gene="yjdP"
                     /locus_tag="ACHUN9_002790"
     CDS             408..737
                     /gene="yjdP"
                     /locus_tag="ACHUN9_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0696140.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(884..1642)
                     /gene="phnP"
                     /locus_tag="ACHUN9_002791"
     CDS             complement(884..1642)
                     /gene="phnP"
                     /locus_tag="ACHUN9_002791"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0696141.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1644..2078)
                     /gene="phnO"
                     /locus_tag="ACHUN9_002792"
     CDS             complement(1644..2078)
                     /gene="phnO"
                     /locus_tag="ACHUN9_002792"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0696142.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2065..2622)
                     /gene="phnN"
                     /locus_tag="ACHUN9_002793"
     CDS             complement(2065..2622)
                     /gene="phnN"
                     /locus_tag="ACHUN9_002793"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0696143.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2622..3758)
                     /gene="phnM"
                     /locus_tag="ACHUN9_002794"
     CDS             complement(2622..3758)
                     /gene="phnM"
                     /locus_tag="ACHUN9_002794"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0696144.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVQDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3755..4435)
                     /gene="phnL"
                     /locus_tag="ACHUN9_002795"
     CDS             complement(3755..4435)
                     /gene="phnL"
                     /locus_tag="ACHUN9_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0696145.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(4546..5304)
                     /gene="phnK"
                     /locus_tag="ACHUN9_002796"
     CDS             complement(4546..5304)
                     /gene="phnK"
                     /locus_tag="ACHUN9_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0696146.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5301..6146)
                     /gene="phnJ"
                     /locus_tag="ACHUN9_002797"
     CDS             complement(5301..6146)
                     /gene="phnJ"
                     /locus_tag="ACHUN9_002797"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0696147.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6139..7203)
                     /gene="phnI"
                     /locus_tag="ACHUN9_002798"
     CDS             complement(6139..7203)
                     /gene="phnI"
                     /locus_tag="ACHUN9_002798"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0696148.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7203..7787)
                     /gene="phnH"
                     /locus_tag="ACHUN9_002799"
     CDS             complement(7203..7787)
                     /gene="phnH"
                     /locus_tag="ACHUN9_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0696149.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7784..8236)
                     /gene="phnG"
                     /locus_tag="ACHUN9_002800"
     CDS             complement(7784..8236)
                     /gene="phnG"
                     /locus_tag="ACHUN9_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0696150.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8237..8962)
                     /gene="phnF"
                     /locus_tag="ACHUN9_002801"
     CDS             complement(8237..8962)
                     /gene="phnF"
                     /locus_tag="ACHUN9_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0696151.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8983..9762)
                     /gene="phnE"
                     /locus_tag="ACHUN9_002802"
     CDS             complement(8983..9762)
                     /gene="phnE"
                     /locus_tag="ACHUN9_002802"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0696152.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9888..10904)
                     /gene="phnD"
                     /locus_tag="ACHUN9_002803"
     CDS             complement(9888..10904)
                     /gene="phnD"
                     /locus_tag="ACHUN9_002803"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0696153.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10929..11717)
                     /gene="phnC"
                     /locus_tag="ACHUN9_002804"
     CDS             complement(10929..11717)
                     /gene="phnC"
                     /locus_tag="ACHUN9_002804"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0696154.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11850..12293)
                     /gene="yjdN"
                     /locus_tag="ACHUN9_002805"
                     /pseudo
     CDS             complement(11850..12293)
                     /gene="yjdN"
                     /locus_tag="ACHUN9_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(12451..12786)
                     /locus_tag="ACHUN9_002806"
     CDS             complement(12451..12786)
                     /locus_tag="ACHUN9_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0696155.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2575 a         3656 c         3901 g         2815 t
ORIGIN      
        1 aatactgagc acaatttatc aatgcaaccg gtgtttcttt ttaaaaaacg taccggagca
       61 atgggcggtc atgtcgctgg caccgcagga ttctgcgttg ctgtgccagc cttttattca
      121 tggtcaatga gtgaggtgat tgataaagac accaggtcgt cagagcgcac tgattatccg
      181 cattatgcca gtgaagttgc actggctttt ggttcacgag tggttggcct cgaactggca
      241 aaaatgattg tggatgcgtg gctgggcgca cagtacgaag gcggtcgtca tcaacaacgc
      301 gtggaggcga ttacggcaat agagcagcgg agaaattgag attcatccac tacttgcatg
      361 gatgagtaat gactaatgtg gatagagttt ctttttgagg ttggctaatg aaacgctttc
      421 cgctatttct tttattcacc ctgctcacgc tgtccaccgt tccggcccag gctgatatta
      481 tcgacgatac tattggtaat attcagcagg cgattaatga cgcttataac cctgaccgtg
      541 ggcgagacta tgaagattcg cgcgacgacg gctggcagcg tgaagtgagc gacgatcggc
      601 gcagacaata tgacgaccgc cgccgccagt ttgaagatcg ccgcaggcag ctggacgatc
      661 gccagcgcca gcttgatcag gagcgtcggc aactggagga tgaagagcgg agaatggaag
      721 atgagtatgg gcgatgaggt tgggtatgga gcaggcatcg cccctcaccc caatggggag
      781 agggagaaaa cgagcgcaat attcaatatc gagcacaatc ggtcccctcg cccctctggg
      841 gagagggtta gggtgagggg gccagcgcgc acattccctc tcatcacgcc accccaatct
      901 ccatcccatc aaaccccacc tcaaaccctg acggtagtgc gttttccatc agccacgcat
      961 caaactggtg gctgatatgg gtcagaatca cccgtggcga gcggataacc tgattcagcg
     1021 caagcacggt atttaaatca cagtgattac gcggtgcatc cgcgcgcggc gggtgactgc
     1081 aatccatcac cattacctgc ggctgattat tgcgtaaaaa tttcagcgtt ttttccggca
     1141 agccagcggt gtcagacagc cacgccaccc ggctgtgcgc cgtttccagt aaatagccga
     1201 aagtgagttt tgagtggtta agcggcagcg gcgtgacctg caacccctgc aaatcaaaca
     1261 ccacaaacgg ttccaccgtg tggctgaaat caagcagccc gggatgttta aacagatcgt
     1321 cgcagccctg ttcatccggc gggccgtaga tcgggatcgg atcgcccacg ccccagcgca
     1381 gcggaaacag cccctggacg tgatccatat gataatgcgt cagcaaaaac tgctggaacg
     1441 atccgggaga ccagcgatcg gtgagatcgt gcagcccggc gtcgatcagg gtgatcgcct
     1501 cgttaaactt cactacgccg ctgcacggtt ggcggcgata ctgcggcgag cgccgcgctc
     1561 ggtcgcaggc cgcgcactcg cagccccatg ccggaacgcc ctgtgcgccg ccggtgccgg
     1621 tgagcgtgag ggtcagactc atgttacagc gccttggtga agcggaagtg actctgctcg
     1681 tagccttctc gcagatagaa acggtgcgcg tcgtggcgct tcacgttggt cgaaagctcg
     1741 gtcatttcgg ccccggcctg gcgggcttct tcttctgccc acgccagcaa cttgctaccg
     1801 acgttcaggc cgcgcgcctg cggcatcact accagttcct gaatttcgcc gatccagttg
     1861 acatgatgca gatgaaactg caaatgcagg ccgatcatgc cgacaacctc gccatccagc
     1921 agcgccagat gatagcgcat gttcgggtcg cgcagattgg cgctaaagcc cacgcgaaat
     1981 gcgtggtggt caaactccgc ctgttttagc tcacaaatca gcgcgtaaac cgcgtcggta
     2041 tcatactgcg tggccgggcg aagctcacaa gcaggcatgg tgtttctcct tctgatgaat
     2101 cagcgtcagc agcgtgtcga ccgactggcg caggctgccg tcattgttga gcgtatggca
     2161 atcctgtgga gtatagcggg cggcgcgcgc caggcgggcg ttaatttcac tggcgttttc
     2221 acggccacgg ttttccaggc gctggcggag gatctccggc gaaacctgta aacagacggg
     2281 cagcagcgcc gattgatagc gcgcccgcgc ctgcggcaga tgggcgcgtg agccgttgac
     2341 cagcacgtcg aatccggcgt gcagccagag gtcgatctcg acgccgacgc cataatacag
     2401 accgttagcg tgccagctta aggccagtag gttctgcccc gcgcgggtaa aaaactcctg
     2461 ctcgctcaga gcgatatggt tttcgcttcc ggcgctggcg tcgcgcgtga tgtagcgatg
     2521 cgccaccagt agctgagttt gttcccgcag gcggagttcc gccagcaggc tatctttccc
     2581 ggagccggac ggccccatta accaaatcag ttttcccatc atcagaacac ccttttaccc
     2641 tgacgccaga cgtggtcgat atgaatgtga ttgcccttgc gatgcgccag caccaagtcc
     2701 gcgcgtttgc cctcgccaat caccccgcga tcctggagat taagcgcctg cgctggattt
     2761 ttagtcacca gcttcaccgc ctgcggcagc gtaaagcggt tgctctcgtc atcggcgacg
     2821 cgaaatgccg catcgagcag gctggcgggg tagtagtcgg aagagaggat atccagcagg
     2881 ccaagctgcg ccagttcact ggctgccacg ttgccggagt gcgagccgcc gcgcacaata
     2941 ttcggcgcgc ccatcagcac gttcatgcca tgcttgcgcg aggcttccgc cgcttcgaac
     3001 gtggtgggaa attcggcgat cacgctgcca agctggtgag attcagcaac gtgggcgtgg
     3061 gtggcgtcat cgtggctggc aagcgcaatt tttcgcgcgc ggcacagggc ggcgatggat
     3121 tcgcgattcg gctgcgacca gcgtgcggcg agcgccagtt gctcttcttc gtactgctgc
     3181 atctgcgcat cagtgaggga gtatttgccc tgataatatt cgcgatactt ctcgcgattg
     3241 gcgaactggc gctggcccgg cgagtggtcc atcagcgaca ccagcgtcac cggctcgcgc
     3301 tgcaccagtt tttcaaacag cggcagcgtg gtgtgatgcg gcagttcgca gcgcagatgc
     3361 agacggtgct cggcgcggtt gacgccgcgt ttctgcgtct cttcgatggc gttgatcatc
     3421 ttctccagat tcaccagccg atcgccgccg tcgcgcacgt cgccaattgc cacggcatcc
     3481 tgtacggtgg tgatgccgct cgccaccatc agcgcgtcgt ggctgctcat cgccgagtgg
     3541 gcaggccagt caactttcgg gcgcggggtg aagaatttat ccagattatc ggtatgcagc
     3601 tcaatcagcc ccggcagcag ccagccgcct tcgccgtcca tcgcctccgg caggcggctc
     3661 tggctttcgg caaaggcgcg gatttcgccg tcctgcacct ccagtgaacc gtgtaccact
     3721 tcgttttcca gcaccaattt gacgttattg ataatcatga agaggcttcc attgggtgca
     3781 ggcggtcggc aacgtcatta cgtacggctt cgtcgtggaa gatgccgacg atggccgcgc
     3841 cacgggcttt ggcttcgtga atcagctcga ctacggcggc gctgtttttg gcgtccagcg
     3901 aggcggtagg ttcgtcgagc agcagaatgg ggtagtcgac gataaagccg cgggcgatgt
     3961 tgacgcgctg ctgttcgccg ccggagaagg tcgatggcgc cagatgccac aggcgttccg
     4021 gcacgttcag gcgggtgagc agacgcgcgg ctttagcggc gcaggcttca cgcggtacgc
     4081 cggtatcgag tagcggctgc atcaccactt ccagcgccga aatacgcggg atgacgcgca
     4141 gaaactggct cacccagcca acggtggttt tgcggatctc caccactttg cgcgctggcg
     4201 cggtgaccag atcgacccat tcgtcaccgt gtttgatctg gatttgccct tcgtcgggca
     4261 gatagttggc gtacagcgag cgtagcaggg ttgattttcc gctgccggaa tggccgtgga
     4321 gcaccacgca ttcgcccgcg ttgacgatga gcgaggcgcg gttgaggacg ggcaggcgca
     4381 cgccgttttg ctggtgcagg atgaaggttt tactgacgtt ttgtacgtta atcatttttc
     4441 gcctcgtggt tcgggtgtcg gatgcgacgc tgacgcgtct tatccgacct acggggagcg
     4501 catttgtagg ccggataagg cgtttacgcc gcatccggca ccggctcaat tctgcaaaac
     4561 cgatgacacc agcagctgtg tatacggatg atgcgggtcg tcgagcacgc ggtcggttaa
     4621 cccactctcc accacttgcc cctgcttcat caccagcaaa cggtccgcca gcaggcgggc
     4681 gacgcctaaa tcgtgggtga caatcactac cgcgaggttc agctccacca ccagtccgcg
     4741 cagcaggtcg agcaggcggg cctgcaccga cacatccagc ccgccggtcg gttcatccat
     4801 aaacaccagc ttcggatgcg tcaccaggtt gcgggcaatc tgcaaacgct gctgcatacc
     4861 gccggaaaag gtggtcggca ggtcgtcgat acggttggcg ggaatctcca cctcttccag
     4921 ccacttctgc gcggtagcgc gaatatcgcc gtaatgtcgc gccccggtcg ccatcagccg
     4981 ctcgccgata ttgccgcctg ccgacacctg gcggcgcagg ccgtcgagcg gatgctgatg
     5041 caccacgccc cattcggtgc gcagcaggcg acggcggtcg gcctcgctca ttgcatagag
     5101 ggaacggttt tcatagtgaa tctccccctg ctgcggcgtc agacgcgcgg agatcgactt
     5161 cagcagcgtg gtcttcccgg agccggattc cccgacaatg cccagcactt cccccggcca
     5221 taaatcaaaa gagacatcgc taaagccttt gcccggcgcg taaaggtggg tcaggttatt
     5281 gaccgaaagt aacggttgat tcattggctt ttcgcctcgc tctgttggcg gcaataatcg
     5341 gtatcggagc agacaaacat gcggtttccg gcgtcatcca gcaccacttc atcaagatag
     5401 ctgtgggtcg atccgcagat ggcgcatggc tcatcccact gctgaacggt gaacgggtga
     5461 tcgtcgaaat cgagactttc cacgcgggta aacggcggca ccgcatagat gcgcttctcg
     5521 cgccctgcgc cgaacagctg cagggcgggc atcatgtcca ttttcgggtt atcgaatttc
     5581 gggatcggcg acgggtccat cacgtagcgc ccgttcacct tcaccggata agcataggtg
     5641 gtggcgatat gaccgaagcg ggcgatatct tcatacagtt tcacctgcat cacgccgtac
     5701 tcctccagcg cgtgcatagt gcgggtctcc gtttcgcgcg gctcgataaa gcgcagcggt
     5761 tccgggattg gcacctggaa gataatgatc tgatcttcgg tcagcggcgt ttcggggatg
     5821 cggtgacgcg tctggataag cgtcgcatcg tccgtacatt cagtggtgtt taccccggtc
     5881 acgcgcttaa agaagttgcg aatcgacacg gcgttggtgg tgtcatccgc accctggtca
     5941 atcaccttca acacgtcgct ttcgccaatc acgctggcgg tgagctggat accaccagtt
     6001 ccccagccgt acggcatcgg catctcgcgc ccgccaaacg gtacctgata accggggatc
     6061 gccaccgctt ttaagatggc gcggcggatc atgcgtttgg tctgctcgtc gaggtaggca
     6121 aagttgtagc cgctcagatt agccatggtt ctgctcctgt tgcagacgtt tgagtagctc
     6181 cagttcggcc tggaaatcga cgtagtgcgg gagtttgagg tgcgagacaa agcctgcggc
     6241 ttcgacgttg tcggcatgcg ccagcacgaa ctcttcatcc tgcgccgggc ctgtcgcgtg
     6301 ctcgccgtat tccggggctt gcaacgcacg gtcaaccagc gccattgcca tcgctttgcg
     6361 ctcgctcatg ccgaatacca gcccgtagcc gcgcgtgaag tgcggcggct catccggcgg
     6421 gtcgataaaa ccgttgacca tttcacactc ggtcatcagc agttcgccga cgtttaccgc
     6481 aaatcccagc tcttccggca caatcgacac gtcgatgtaa ccactgcgga tctcgcccgc
     6541 gaacgggtgg ttgcgcccgt aaccgcgctg ggtggagtaa gccagcgcca gcaaatagcc
     6601 ttcgtcgccg cgcattaact gctgcaaacg ggaagaacgt gaacaggggt aaaccggcgg
     6661 cgtgcgggtg atgtcatccg gctgtgctcc gctatcctct tcaaacttcg ccagcccctg
     6721 acgcgccagc aggctgaaaa cgtgcggcga cggctgttgt tcgctgtcgg cggtggtcag
     6781 cgtcggcgct tcgccgtttg ccagcagagt gaaatcgagc aggcgatggg tgtagtcgta
     6841 ggttgggcca agcagctggc cgccgggaat atctttataa accgctgaaa tacgccgttc
     6901 gagacgcatc tccgtggtgt cgagcggctc gcttaccgcc agtttcgtca gcgtggtgcg
     6961 gtaggcgcgt agcaggaaaa tcgcttcgac gttatcgccg ctggcctgtt tcagcgccag
     7021 cgccgccagt tcgcggtcgg caatgccgcc ttctgtcatc acgcgatcca ccgcgaggtt
     7081 aagctgctgt tcaatctggg cgacgctcag ttcgggcaaa tcggtatcgc cccggcgtcg
     7141 gctctcttgc agggcgtggg cggcgtcgat cgccttttcg ccccctttca cggcaacgta
     7201 catcagcaca cctccacatg agtggttcgc gggatagcca gcaggcgctc gccgcaggtc
     7261 aggattaggt cgatgccgag cgggaacggg tgcgggcgct cggtgagttc gtgcagaatg
     7321 cactctggca gctgcggagc gatcattcgt tcttcggcaa tgcccgcgcc ggtgagacgc
     7381 aacatgcgtc cgccgctcag gctggcgacc tgtaaaatca gcgtcgcgcc cgcttccggc
     7441 gcaacggcgg tgtcggtgga aagggcgttg agctgttcgc tggaaatcgc ctcatccgtc
     7501 accgcgaagg tcgcctgttc cggctggctg accagcggcg cgttggtatg aaaacgcagg
     7561 ctctgattga cgatatcgtt acttaatggc gtggcaagcc acaccggcgt gtcgttatcg
     7621 gccagcgtca gcagcacgct ggtggtggcg atattcagcg gctgccagcc gcgtttgagc
     7681 tgatacaggg cgacaatcac gcccggctcg ctcatggcct ttaacaggcg acgaaaactg
     7741 tgctgggcat cctgcacggg aagcataaaa gcggtttcca gggtcatgcg ttgtctccgc
     7801 gaaccatcgt aaagaagtcg acccggctgg cgttcacttc ggcctggcgt gcggcaatgc
     7861 gtgccatacg gtcagcgtcc agcggggcaa taagggtttc tgataagttt tgaaagtggc
     7921 gagattgctg catcagcgca tcaatcagcg cgcagcgttc ggcgtgctgt ttatcacgcc
     7981 ccagcaccca gctgtagccg agggtgccgt cagtcaggcg tacggcggca cgggtcagcg
     8041 tggcgtcgcc ggcaaaaaag cgttcgccgg taccgcccat tcgcgcctga atctgcacca
     8101 ggccagtttc ggcagtgcgg atcacttcat agtcggcggt gatgttcagc gcgttcaggc
     8161 gggctgccag ttcagccggt tggctgtgcg ccagtacgga catccagtgc tggcgggtcg
     8221 cggtatctgc gtgcattcag tgctccatag tgaattcaat catgtcggcg cgcgtcaggc
     8281 tgacggagta ctccgccggg ctgctttcac cgtcacggtg gttaagggtg cgcacgcaca
     8341 gcagcggcga catattcggg atttcaaggc gctggcactc tttggcctgg gcgcggcggg
     8401 cgctgatccg cgtctggctg cggcgcagcg caattccggt ttgctcgcgc agaaaatcgt
     8461 gcagcgaacc gctgtcgaaa cgttgcagtg tcggccagag ggtgaggtcc gcaaagtagt
     8521 ggtcgattaa acagagcgcg acgccgttga cgcgacgcag ggtgcgcaaa tggataacgt
     8581 tctcaccctc ggtaatcccc agcgcgtcag caacgtgacc ggacgcgggg cgcaataccg
     8641 acagcagttt ttcgctggtg ggatggctgc cctggtccag caggttctgg ctaaaacgcg
     8701 cctgggcgtt gagcgggtaa tcgaacgggc gcatcagcac cagcacgccg atgccctgac
     8761 ggcgctgtac ccagcctttt tccaccagtt ggtcgatggc gcggcgcagg gtgtggcggt
     8821 ttacctcaaa gcgcgccgcc agttgctgct cggcgggaag atagtcgccg cagcgatagt
     8881 gttgacgaag ctcctgctca agttttgcgg ctatctcctg atagcgtgtt gggtagctgg
     8941 tcggatgtgt agacaagtgc atagatatca atgcctcact tatcagataa agtgcttacg
     9001 caaccgttga gagaggaaat ccagcaggct gaccgtgacg atgataagca ccatcagggc
     9061 gcaggtttgt tggaactgga aaccgcgaat cgcttcccac agggtgacgc cgatcccgcc
     9121 tgcaccgacc atgccgacga cggtcgccga gcggacgttg gattcgaagc gatagaggga
     9181 gtaggagatc agcagcggca ttacctgtgg cagcacgccg taaaggatct cttccagctt
     9241 gttggcgccg gtggcgcgaa tgccttccac cgggccgggt tcaatcgctt ccaccgcttc
     9301 ggaaagcagt ttcgagagca cgccggtggt gtgaataaat agcgccagca cgccagcgaa
     9361 cggtccgaga ccgacggcga ccacgaacag catggcgaag accatttcgt taatggcgcg
     9421 gcaggcgtcc atcaggcggc gaacgggctg gtaaacccac cacggcacca ggttttcggc
     9481 gctcatcagg ccgaagggga tggagagaac cactgccagc gcggtgcccc agacggcgat
     9541 ttgcagcgtg acggccattt cggtgaggta atcctgccac tggctgaaat cgggcgggaa
     9601 gaagtcggcg gcgaaggtcg ccatgttgcc gccgtctttg atcagcgtaa gcggggccat
     9661 ttccgcgccc tgccacgaga cgaccaacac ggcgagaaca atggcccagc tcagaagcga
     9721 gaaccagctg cgcttgggtg gggcgatggt gatggtttgc atgtttggct ccgtttaatg
     9781 ttttctccct ctccctgtgg gagagggccg gggtgagggc atcagcgtgc gcatttcccc
     9841 ctcaccctaa ccctctccct caagggagag gggaccgatc gagcacatta ctgcaccgct
     9901 ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc atccagctgc
     9961 gcctgaatct cggtggtttt cgccagcttg tcctgctcat tcagtccgtt attgcttttt
    10021 acgccctgaa tctctttaaa cagtgcgagc tggcgaatcg gcaccagttg caggtcgctg
    10081 gaggcgcgaa acggtgccca gcccaggcgt tccagcaccg ctttctcttc cggcgttttt
    10141 ccgtagttca taaagaagtc gtagatcttg tctttggtgg tttcggagag attcttgcgc
    10201 cagacgatcg gatcgcctgg gatcagcggc gacttccaga tcaccttcag ttctttcagc
    10261 ttctctggcg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt ggcaacatcc
    10321 acctgcttgt tggcgacggc cagcgcgttg gtttcatgcc cggcgttgac ggtgcgctta
    10381 aagtcgctgg cggagatatt gtttttggca aagacgtagt aaccggggac gaggaagcca
    10441 gaggtggagt taggatcgcc attgccgaag gtgagatctt tccgcttcgc cagcagatcg
    10501 ttcaggttgt tgatcggact atctttgttg acgatcaaca cgctccagta acctggcgat
    10561 ccatccgccg cgaccgtctg ggcgaagacc tggccgttgg cgcgatccac cgcttccatt
    10621 gccgacaggt tgccgtacca ggcgatatcc actttattga agcgcattcc ctggataatg
    10681 cctgcgtagt ctggggcaaa gaaggcgttc accttcacgc ccagcttctt ctccatatcc
    10741 tgtaagaatg gcgtccattg cggtttcagg ttttgctgtg attccgttga aataatgccg
    10801 aaattcagcg ccttttcctg ctcttcggcg tgtgccgggc ttaacagggt gctgaggctg
    10861 aacatgctgg tgaaggccag cgaggcaatt atcttagcgt tcattcgttt tcctcaatga
    10921 tggggatgtc aggcagcttt cgcgttctct tcgatgcgat taatgctgcg gtagagatgg
    10981 tcaaaacgtt cgttatcaaa ctgttggctg ctgccgtcgt agaaaacgtg cccctggcgc
    11041 agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag cgtgacgacc
    11101 acggtgatgc cgtcgttctg attgatgtcg cgcagggtgt ccatcacgat gccggcggat
    11161 tccgggtcca gcgaggcgat gggttcatcg gccagaatca ccttcgcctg ctgcatcagc
    11221 gcgcgggcaa tcgccacacg ctgctgctgt ccgccggaga gggtggaaac gcgttgatgg
    11281 gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg cgcgttgttt ctgctcgcgg
    11341 gtaaaccagc taaaacaggt gcgccagaac ggcgtgctgc cgagtgcgcc aatcagcacg
    11401 ttctccagta cgctcaggcg gttcaccagg ttgaattgtt ggaagatgta gccggtgttg
    11461 gcgcggcttt tgcggatatc gcgcgccaga cggccttcgc gctggactgt gcggcccagc
    11521 agctcgatat ggctgccggc ggatttatcg ccggtaatca aaccgcttaa gtgacgtaaa
    11581 agggtggatt tgccggaacc cgacggccca agcagagcca ccatttcacc gtgatgaatg
    11641 ttcagatcaa ccgcatgcag cgcctgatgc tgattgaagg ttttggcgag cttctcgaca
    11701 cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt ggcggattat
    11761 tgtgacgatt tggttaatta aaagtgacta acagatgaag agttaacggg aattcgatga
    11821 cagtgcggga gggcgggcct cccggcgggt tattgcgttg gttgttgttt gacgacatta
    11881 atcatccacg gtacgccaaa tttatcggtg actttgccaa agccatgcgc ctagaaagtt
    11941 tcctgccagg ccatttcgat ttttccgtta gcggcaagat tgtcaaacca gcgttttcct
    12001 tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc ttttcctgac
    12061 ggcatggcat cgctcatcat gatgtcgctt ccggcaatgc gcacattggc gtgggcgatg
    12121 gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg cgctgatttt
    12181 ggcatttcgc cgaagctgat tttatagagc agttcagcgc ccaacgtacg ttgataataa
    12241 gcaattgcgt cggcacagtt accggcaaaa gagaggtagg gacttaacgg catgatggtg
    12301 acctcagtta agagaagcca attaagtgta gttcgaaatt tatacagatg agaggctgcc
    12361 tgataagacg cgcaagcgtc gcatcaggca gtcggcatcg ttgccggatg cggcgcgagc
    12421 gccttatccg gcttaccgat cacaatacaa tcagttcttt ttcacaaact cagatttcag
    12481 tttcatcgga ccaaaaccgt cgattttgca atcgatgtta tggtcgcctt caaccaggcg
    12541 gatgtttttc actttggtgc caattttcag catcgaagag ctacctttca ccttcagatc
    12601 tttaatgatg gtaacgctgt cgccgtcagc cagcagattg ccgttagcat ctttaacgat
    12661 cagctcgtcg ctttcctgtg caggttctgc gtcgttccat tcgtaggcac attccgggca
    12721 gatgtacatg ccgttatctt cgtaagtgta ttcggagttg cattttgggc agtgtggtaa
    12781 tgacatgtgg atttcctcaa aagtcagcag ggcaaaagcg ccaaaaaacg gcagattgcc
    12841 gaaaaaggcc gcaattatac acaaaatccc tgagtttgtc gggggtactg ctgattttag
    12901 acgacgatcg ttttcatttg aaaggaaacg caaaaagaaa ataatct
//
LOCUS       DAZOYS010000115        12856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOYS010000115 DAZOYS010000000
VERSION     DAZOYS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.115"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUN9_002807"
     CDS             complement(<1..330)
                     /locus_tag="ACHUN9_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0696156.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUN9_002808"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUN9_002808"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0696157.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUN9_002809"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUN9_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0696158.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUN9_002810"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUN9_002810"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0696159.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUN9_002811"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUN9_002811"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0696160.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUN9_002812"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUN9_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0696161.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUN9_002813"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUN9_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0696162.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPVSEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUN9_002814"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUN9_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0696163.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUN9_002815"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUN9_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0696164.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUN9_002816"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUN9_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0696165.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUN9_002817"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUN9_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0696166.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUN9_002818"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUN9_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0696167.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUN9_002819"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUN9_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0696168.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3002 a         3549 c         3106 g         3199 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactgac aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gagggggggc tgcgattttc aataatgtgt
    12601 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
    12661 ttgggtgtat ttcaatatgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
    12721 ggaataatcg aaatgggatg ttgcgcacag ttaaaataaa tcaccgtaaa taatcatctg
    12781 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
    12841 acaacaaact aattaa
//
LOCUS       DAZOYS010000116        12825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOYS010000116 DAZOYS010000000
VERSION     DAZOYS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.116"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUN9_002820"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUN9_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0696169.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUN9_002821"
     CDS             433..621
                     /locus_tag="ACHUN9_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0696170.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUN9_002822"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUN9_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0696171.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUN9_002823"
     CDS             816..989
                     /locus_tag="ACHUN9_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0696172.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUN9_002824"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUN9_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUN9_002825"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUN9_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0696173.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUN9_002826"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUN9_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0696174.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUN9_002827"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUN9_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0696175.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUN9_002828"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUN9_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0696176.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUN9_002829"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUN9_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUN9_002830"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUN9_002830"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0696177.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUN9_002831"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUN9_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0696178.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUN9_002832"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUN9_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0696179.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..>12825
                     /locus_tag="ACHUN9_002833"
     CDS             12531..>12825
                     /locus_tag="ACHUN9_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696180.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYS"
BASE COUNT         3143 a         3246 c         3330 g         3106 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcag
//
LOCUS       DAZOYS010000117        12804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOYS010000117 DAZOYS010000000
VERSION     DAZOYS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.117"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHUN9_002834"
     CDS             complement(178..1065)
                     /gene="rarD"
                     /locus_tag="ACHUN9_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0696181.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHUN9_002835"
     CDS             complement(1117..1584)
                     /gene="yigI"
                     /locus_tag="ACHUN9_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0696182.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1749..2618
                     /gene="pldA"
                     /locus_tag="ACHUN9_002836"
     CDS             1749..2618
                     /gene="pldA"
                     /locus_tag="ACHUN9_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0696183.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2751..4580
                     /gene="recQ"
                     /locus_tag="ACHUN9_002837"
     CDS             2751..4580
                     /gene="recQ"
                     /locus_tag="ACHUN9_002837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0696184.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4644..5261
                     /gene="rhtC"
                     /locus_tag="ACHUN9_002838"
     CDS             4644..5261
                     /gene="rhtC"
                     /locus_tag="ACHUN9_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0696185.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRTRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5323..5943)
                     /gene="rhtB"
                     /locus_tag="ACHUN9_002839"
     CDS             complement(5323..5943)
                     /gene="rhtB"
                     /locus_tag="ACHUN9_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0696186.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6054..7076
                     /gene="pldB"
                     /locus_tag="ACHUN9_002840"
     CDS             6054..7076
                     /gene="pldB"
                     /locus_tag="ACHUN9_002840"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0696187.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7084..7884
                     /gene="yigL"
                     /locus_tag="ACHUN9_002841"
     CDS             7084..7884
                     /gene="yigL"
                     /locus_tag="ACHUN9_002841"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0696188.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7960..8859
                     /gene="bioP"
                     /locus_tag="ACHUN9_002842"
     CDS             7960..8859
                     /gene="bioP"
                     /locus_tag="ACHUN9_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0696189.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8747..9700)
                     /gene="metR"
                     /locus_tag="ACHUN9_002843"
     CDS             complement(8747..9700)
                     /gene="metR"
                     /locus_tag="ACHUN9_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0696190.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9936..12197
                     /gene="metE"
                     /locus_tag="ACHUN9_002844"
     CDS             9936..12197
                     /gene="metE"
                     /locus_tag="ACHUN9_002844"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0696191.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12319..12441)
                     /locus_tag="ACHUN9_002845"
     CDS             complement(12319..12441)
                     /locus_tag="ACHUN9_002845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696192.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12482..12727)
                     /locus_tag="ACHUN9_002846"
     CDS             complement(12482..12727)
                     /locus_tag="ACHUN9_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0696193.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3011 a         3227 c         3430 g         3136 t
ORIGIN      
        1 ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt aagtactggg
       61 gagttttggg ggtatttcct gtaacaaata ttgttctttt tcttatctta atttttttca
      121 aaaaaagata aataagtcag tgggtaggct ctttaaacgg cctacccatg ttaattctta
      181 actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg ccacccaaat
      241 aaaggcgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg tcacagccag
      301 caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga gacgcaagcg
      361 cgtggcagcg gcggtaaaac acaacagcgg tacggtagtg acaataccgg cggcgatcag
      421 cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt cggcaatagc
      481 aaacaggtaa attgccgcca cgggcagcag ccacatggtt tcgattaaca tgccggtttg
      541 cgcttcaacg gcaatcttct tgcgtaccag accgtagaag gcaaaactaa atgccagtcc
      601 cagcgcgata ataggtagcg aaccaaaagt ccacagctgg actaacacgc cacatatcgc
      661 cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa tcatccccag
      721 cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca tatggtgatt
      781 gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag agactgccag
      841 cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact ggcggcaaat
      901 gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca ggatttcatc
      961 ggcgggcacg tagtaaatca acttgaagta cgctggcgct ataccccaaa taaaataagc
     1021 ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga atactcattt
     1081 ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat aggtggcggt
     1141 ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga cggcgacttt
     1201 attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc ctgggcgcag
     1261 ataatcaacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta gttcatcttc
     1321 actgatggtt tcgtggcggg ttaaggtact tcccacgcac accagaccgg cggcgacatc
     1381 cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc ccaccatcat
     1441 tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca gttccatccc
     1501 caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt tcagggcttg
     1561 ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat gaaatgttga
     1621 ttttatgctt ctttgttgtt ggtttctact ttaggaaggg ataactaacg gctatggagt
     1681 taagtatgta aaatagcccg cagaaaaata ttcaccttat caataattcg ttacggagaa
     1741 cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg cctatggcag
     1801 tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg cgtggcagta
     1861 ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct tatgacacca
     1921 actacctcat ttacacccaa accagcgatc tgaataaaga agcgattgcc agttacgact
     1981 gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca tttccgctgt
     2041 ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa aaatcctggt
     2101 ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac gaaccgcaat
     2161 tgttcctcgg ttttgccacc gattactgtt ttgcaggttg gacgctgcgc gatgtggaga
     2221 tggggtataa ccacgactct aacgggcgtt ccgacccgac ctcccgcagc tggaaccgcc
     2281 tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag ccgtggtatg
     2341 tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac taccagctta
     2401 aaatcggcta tcacctcggt gatgcggtgt tgagtgcgaa aggacagtac aactggaata
     2461 ccggttacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat gtgcgccttt
     2521 atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc aaccagaccc
     2581 gtgtcggtgt aggagttatg ctaaacgact tgttttgatg aacggttgag tggttggcaa
     2641 atctggaacc cagcatccag gattaccctc tcagagacta aaagcattgc agtttctcgc
     2701 gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat gtggcgcagg
     2761 cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc tttggctacc
     2821 aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt cgcgattgcc
     2881 tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct gccttattgc
     2941 taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat caggtggatc
     3001 aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc cgcgagcagc
     3061 aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat atcgccccgg
     3121 aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg gtgttattag
     3181 ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg gaatatgccg
     3241 cgcttggtca gttacgccag cgtttcccga ccctgccgtt tatggcgctg actgccacag
     3301 ccgacgacac cacgcgccag gatatcgtgc gcttgctggg gctgaacgat ccgctgattc
     3361 aaatcagcag ttttgaccgt ccgaatattc gctacatgct gatggagaaa ttcaaaccgc
     3421 tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt atctactgca
     3481 acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt attagcgcgg
     3541 cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag aaattccagc
     3601 gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc aataaaccaa
     3661 acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat tatcaggaaa
     3721 ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat gctgttttac gatccggctg
     3781 atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg caggatattg
     3841 agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc cgtcgtctgg
     3901 tattgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc gatatctgcc
     3961 tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt tccaccattg
     4021 gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga agtgattcgt ggtgctaata
     4081 accagcgtat ccgcgactat ggtcatgaca aactgaaagt ctatggcatg ggccgtgata
     4141 aaagccatga acattgggtg agcgtgatcc gccagctgat tcacctcggc ctggtgacgc
     4201 aaaatattgc ccagcattct gccctacaac tgacagaggc cgcgcgcccg gtgctgcgcg
     4261 gcgaatcctc tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg aaagcgatgc
     4321 agaaatcgtt cggcggcaac tatgatcgca aactgttcgc caaattacgc aaactgcgta
     4381 aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac gcaaccttga
     4441 ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt aacggcgttg
     4501 ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc gctgattcgt gcgcatgtcg
     4561 atggcgacga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag actccgtaaa
     4621 cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc atggtgcaca
     4681 ttgtggcgct tatgagcccc ggtcccgatt tcttttttgt ctctcagacc gctgtcagtc
     4741 gtacccgtaa agaagcgatg atgggcgtgc tgggcattac ctgcggtgta atggtttggg
     4801 ctgggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg ctgcatacgc
     4861 ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgctg cgcggcgcgc
     4921 tgaaaaaaga ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa agtgggcgca
     4981 gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc tactttggct
     5041 cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg ggcatttttg
     5101 cgctgatcat tgtcgaaacg ctggcgtggt ttatcgtcgt tgccagcctg tttgccctgc
     5161 cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt
     5221 tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga
     5281 gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga
     5341 tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat
     5401 ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt aaccgatcat
     5461 cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg
     5521 ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt
     5581 ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag
     5641 cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc aaatcaagta
     5701 agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa atagcgtccc
     5761 caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga
     5821 cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga
     5881 gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt
     5941 catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt
     6001 gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc
     6061 agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc
     6121 tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg
     6181 tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc
     6241 gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat ttggggtttg
     6301 atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc
     6361 atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc
     6421 aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg
     6481 cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg
     6541 cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact
     6601 gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg
     6661 cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac
     6721 gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg
     6781 aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgccaacgc
     6841 ttctcttgca ggctgaagag gaacgcgtgg tggataaccg catgcatgac cgtttttgtg
     6901 aactccgcac cgccgcgggc catcctgtcg aaggaggacg accgttggta attaaaggtg
     6961 cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccattg
     7021 ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt
     7081 cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg
     7141 ttatcccctt acgccaaaga aaccctgaag ctgctcaccg cgcgcggcat caactttgtg
     7201 tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagatcaag
     7261 tcgtacatga tcacctccaa cggcgcgcgc gttcacgatc tggatggcaa tctgattttt
     7321 gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatctg
     7381 gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa
     7441 gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc tggattactg
     7501 gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca actgctgccg
     7561 ctggagcagg cgattaacgc tcgttggggc gatcgcgtca acgttagttt ctctacctta
     7621 acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga agcggtggcg
     7681 aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgatgcc
     7741 gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa
     7801 gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt gccgcattat
     7861 ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt
     7921 cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact tatcatcacc
     7981 acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat
     8041 agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct gccgtttctg
     8101 cgtacccgtg gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag
     8161 ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg
     8221 ttgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag
     8281 cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt
     8341 attcgctatg atcaggttac cgaccatttc tggactggct tgttgctggt gcaactctcc
     8401 aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg
     8461 ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca
     8521 tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg
     8581 gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg
     8641 caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg
     8701 gtaaacctgg ctatctggca ccaacagccg cactggccaa cgttcattac aggcgcgctg
     8761 gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg
     8821 gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta atcggctgac
     8881 gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt
     8941 tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa
     9001 tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg cttttcagtg
     9061 acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca
     9121 ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc gttttcgccg
     9181 ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc ggcgaataat
     9241 gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca
     9301 acgcaggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt
     9361 tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg cactcaatgg
     9421 caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg
     9481 gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct
     9541 ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag
     9601 acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag ccgcagttcc
     9661 gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca
     9721 tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg
     9781 gacggctaaa atccttcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg
     9841 gtcacttact tcagtaagct cccggggatg aataaacttg ccgccttccc taaattcaaa
     9901 atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg
     9961 gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg
    10021 ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg
    10081 atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc
    10141 atgtactgac caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg
    10201 gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac
    10261 ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg
    10321 agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag gaagtggacg
    10381 aggcgctggc gctgggccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt
    10441 ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc
    10501 cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg
    10561 atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt
    10621 acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc
    10681 caaatctcga cacgattact gcgctgcctg ttcagggtct gcatgttgat ctcgtacatg
    10741 gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggttg ctgtctgcgg
    10801 gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat gcgcaaatta
    10861 aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc
    10921 ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg
    10981 ccctacaaaa atgccatgaa ctagcactgc tgcgcgatgc gctgaacagt ggtgacacgg
    11041 cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata
    11101 atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg
    11161 tctatgaagt gcgtgctgaa gcccagcgtg cacgttttaa actgccagcg tggccgacca
    11221 ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa
    11281 agggcaatct cgacgccaat aactaccgca cgggcattgc ggaacatatc aggcaggcca
    11341 ttgttgaaca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg
    11401 acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa aacggttggg
    11461 tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac gttagtcgcc
    11521 cggcaccgat taccgtggag tgggcgaagt atgcgcaatc tctgactgat aaaccggtga
    11581 aagggatgct gactggcccg gtgactattc tctgctggtc gttcccgcgt gaagatgtca
    11641 gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg
    11701 aagccgctgg aattggcatc atccagattg acgaaccggc tctgcgcgaa ggtttaccgc
    11761 tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg
    11821 ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg
    11881 acatcatgga ttcgattgcg gcactggacg cagacgtcat caccatcgaa acctcgcgtt
    11941 ctgacatgga gttgctggag tcgttcgaag agtttgatta tccgaatgaa atcggtccag
    12001 gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga
    12061 agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt taacccggac tgtggcctga
    12121 aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga
    12181 atttgcgtcg gggatgatga atattaacga gtcactgtaa attgcagtac gatgaaagag
    12241 gcaaaactgc ctgatactag gcctacataa tttatgcaat ttattgaatt tttgggtttt
    12301 gttggccgga caaggcgttc acgccgcatc cggcatgaac aacgcgcact ttgccaataa
    12361 tctgatggct tccatcaccg gaagccattt tgctatctgc gcccgttcag atacagaaat
    12421 aaccgaatca tttttacgca aaatataatc ctgagcgatt tgttcagtaa tcaacgctac
    12481 gttatttatt ctcacttttc acgaaattgt atcccttttc agtaacgtgg tagtgaggca
    12541 tagacttatg tccgctatcc tcaataataa ccaaaccctc gttagctaat ttgttaacaa
    12601 tgttaacaac attaccgaat cctggaattt gccttatgga tagtgtgcga tcgagattca
    12661 tccagttcca gtttatatct ttctcagcca taattttcag tacgttaatt tcagtttcag
    12721 ttatcatagt caagtgcatc ctggtggtat aagtcattaa gttctccctt aagtttataa
    12781 tcttctagag tagcagtatg cgta
//
LOCUS       DAZOYS010000118        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOYS010000118 DAZOYS010000000
VERSION     DAZOYS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.118"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUN9_002847"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUN9_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0696194.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUN9_002848"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUN9_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0696195.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHUN9_002849"
     CDS             1797..1970
                     /locus_tag="ACHUN9_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696196.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUN9_002850"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUN9_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0696197.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUN9_002851"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUN9_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0696198.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHUN9_002852"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHUN9_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0696199.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHUN9_002853"
     CDS             complement(4268..5191)
                     /locus_tag="ACHUN9_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0696200.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHUN9_002854"
     CDS             5408..6709
                     /locus_tag="ACHUN9_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0696201.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUN9_002855"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUN9_002855"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0696202.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWALGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHUN9_002856"
     CDS             8771..8995
                     /locus_tag="ACHUN9_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0696203.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUN9_002857"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUN9_002857"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0696204.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUN9_002858"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUN9_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0696205.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUN9_002859"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUN9_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0696206.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUN9_002860"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUN9_002860"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0696207.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2926 c         3146 g         3283 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gctaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZOYS010000119        12170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOYS010000119 DAZOYS010000000
VERSION     DAZOYS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.119"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..71
                     /locus_tag="ACHUN9_002861"
     CDS             <1..71
                     /locus_tag="ACHUN9_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR0696208.1"
                     /translation="YEALRTVDRLLRGQSFIKQADL"
     gene            82..369
                     /locus_tag="ACHUN9_002862"
     CDS             82..369
                     /locus_tag="ACHUN9_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696209.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            381..623
                     /locus_tag="ACHUN9_002863"
     CDS             381..623
                     /locus_tag="ACHUN9_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR0696210.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            620..733
                     /locus_tag="ACHUN9_002864"
     CDS             620..733
                     /locus_tag="ACHUN9_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696211.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            820..1023
                     /locus_tag="ACHUN9_002865"
     CDS             820..1023
                     /locus_tag="ACHUN9_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696212.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            1020..1265
                     /locus_tag="ACHUN9_002866"
     CDS             1020..1265
                     /locus_tag="ACHUN9_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0696213.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            1291..1410
                     /locus_tag="ACHUN9_002867"
                     /pseudo
     CDS             1291..1410
                     /locus_tag="ACHUN9_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1407..1751
                     /locus_tag="ACHUN9_002868"
     CDS             1407..1751
                     /locus_tag="ACHUN9_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696214.1"
                     /translation="MILANDFLEYLLNTERDRYDMYLKSLPVPQLADGKIVIDGRYMI
                     DSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTVLEIQRLA
                     TVCRDTLNGMTK"
     gene            1758..4580
                     /locus_tag="ACHUN9_002869"
     CDS             1758..4580
                     /locus_tag="ACHUN9_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR0696215.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DDSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            4657..5616
                     /locus_tag="ACHUN9_002870"
     CDS             4657..5616
                     /locus_tag="ACHUN9_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0696216.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            5621..5932
                     /locus_tag="ACHUN9_002871"
     CDS             5621..5932
                     /locus_tag="ACHUN9_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0696217.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            5993..6517
                     /locus_tag="ACHUN9_002872"
     CDS             5993..6517
                     /locus_tag="ACHUN9_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696218.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            6514..6753
                     /locus_tag="ACHUN9_002873"
     CDS             6514..6753
                     /locus_tag="ACHUN9_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696219.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(7445..8491)
                     /locus_tag="ACHUN9_002874"
     CDS             complement(7445..8491)
                     /locus_tag="ACHUN9_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0696220.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(8491..10242)
                     /locus_tag="ACHUN9_002875"
     CDS             complement(8491..10242)
                     /locus_tag="ACHUN9_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0696221.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10397..11233
                     /locus_tag="ACHUN9_002876"
     CDS             10397..11233
                     /locus_tag="ACHUN9_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR0696222.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11257..>12170
                     /locus_tag="ACHUN9_002877"
     CDS             11257..>12170
                     /locus_tag="ACHUN9_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR0696223.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQ"
BASE COUNT         3099 a         3294 c         3191 g         2586 t
ORIGIN      
        1 tatacgaagc acttcgcacg gttgatcgtc tgcttcgcgg gcaaagtttt attaaacagg
       61 ctgatttata acaggtgctt tatgaccaaa gacaatgcac aaggtgtatt tatccgtttt
      121 attgattttc gcggtgaact gttattacgt gcatccgcta ttgacggagt gactccggcg
      181 ggtaaaaacg gagccgacga agccacttac gtttatctga acggcacgcg actgcttgtg
      241 gaacttccgt accagaccgt acgagaaatc atcagcgaag atgaaaaggc acgccaggtt
      301 aatggcgatg aaccctatat cgaaattatt tgtatggatt cagaagctga aatacagaaa
      361 gcagattaaa gggcgttgtg atgggcaaag aatataaaac tctcattaac aaagcacttg
      421 agcgttttta ttttcgctta agtgcatcag gcgctcatgc tgaacgtgcg gcccgtgact
      481 cattgaccag agcaatccga agtctgtatg acgtggcttt ttacgctgat gatctggatg
      541 cacttaacga actttccgag ctgatctgtg ccgcagaatg cggggaacat attgaaccgt
      601 ataagctggg aaatatcgca tgagtatatt tatttcatgg cttgttctga ttatttcggt
      661 ggtctgcgcc attgggatta tgcgaattat taattcagta aaaaagattg aacgcttttt
      721 cactgaagaa taaccgcgca aataagaccc caggttaaat aagaaaatgt gaaaacaatc
      781 cgcattcgcg gaggtattcg cacacgccaa ggaggcgtaa tggcaattaa gcattttcct
      841 gtcgttcgtt tcacctccag aggacgtgaa tacgaagttg acgaacgcct gattaccaca
      901 atcgacaaac accgttcaga aaaggatgca catcatatct atctcactga cggcacttac
      961 ttctgcgcca ccaacgtggt gcaggtgaat cttatcagac aggtacagga gtcacgcaga
     1021 tgaccattct ggactacatc gctgccaatc cgggttgtag cagtggagaa atcgccgcag
     1081 cactgaatac accaaccaca accattaatg cggagctacg ccgtctctgg cgcagcggtt
     1141 tagtcacaag aaaagagcgc aaaacaggcg gtcgcttttc ttatcaggta aacctgatgc
     1201 cgtttgggtg tagcaaccca ctaacccaga tgttcaacca gctactgagg gaaatcagag
     1261 catgagcacc tccaactgcc ggaaaccacg tcgggcttca gcagctcatc cggcagcaaa
     1321 acaaactcca ttaattcctg ttccgggcct ttcctgcacc ttgcggcggg aggccttcgc
     1381 acatctgtaa caagaggatt gccgcaatga ttctcgccaa cgactttctt gaatacctgc
     1441 tcaacacaga gcgtgatcgt tatgacatgt acctgaaatc cctgcctgta ccgcagctcg
     1501 ctgacggaaa gattgttatt gatggtcgct acatgattga cagccacgag ggaaattaca
     1561 ggctttaccg cattgaaggt ggcaccccgt ccgttattgg catttaccag cgcccatcct
     1621 ctgcaatcgt cgatgtgatt gccgacagca tccgcatcac acatcgccat gccgacacag
     1681 aagacaccgt gctggaaatt cagcggctgg ctacagtctg ccgcgacacc ctgaatggca
     1741 tgacgaagta aatcactatg acggcagagt acatcaggga ctggcaacaa ccgcgccacg
     1801 cagtggggcg tgaaggaacg gggatccccg ctcctgaatc cgcgctttcc tcctggctgg
     1861 atgcccaccg ggcagagaac gagcgccgcc aggaaatggc tgatgcggcg ttctccgcca
     1921 cgccactggg caacctgatt aataaaagcc tggacgcaca ggaaaaacag gacaaaacca
     1981 tcacactggc aggagacgcc agaaaacagg cacgcggcgc ggtggatgaa gccatggcct
     2041 cgctgcgcct gctgccgtcc tatctgcgcg atccgcttat tcgccacctc tccttcctgc
     2101 gcaaaaaaca ggaagccgat cgccggaaag gcaaaaagag ctggcaggcg gaacgctatg
     2161 cacgcggaac cctgcgcaaa atattcgaac gtctggatcg cactgacgga cactggctga
     2221 caccgggtta tcgctccctt gccggacgtg aacgcctgga cgatttgctt tacctgccgc
     2281 aactcaacaa acaccagata cagacgctgg ccaccatgac ggcggcgatg ttcagcagca
     2341 ccttcgaaaa actctgcgat ggttttggcg cgactgatgg cgagctgacc atggatgtaa
     2401 cgctgaaggc gtatcagatg ctggcccgca tggcgttaca cttacacgcc atgcctccgc
     2461 attatgacgc actgacaaca gacaaagacc ggaggcacga accggacaca gaactgctgc
     2521 cgggcgcaat ccttcgcctg acctgtgcgg aatggtggaa acgcaaactg tggctgttac
     2581 gttgcgagtg gagagaagaa caacttcgcg ccgcctgtct ggtttccaga aaaacatcgc
     2641 cctatctgag ccaggacgcg ttaagcgagt ttcgcgcaca gcgcgagaaa acacgcgatt
     2701 tcctgaaaag tttcatgctg gaaaatgaag acgggttcac gattgatctc gagacggtgt
     2761 attacgcggg agtaagtaac ccggttcacc gtaaggcaga aatgatggcc accatgaagg
     2821 ggctggaact tctggccgaa gcccgtggcg acaaagcggt gtttctgact gtcacctgcc
     2881 cgtcaaaata ccacgccaca acggagaacg gtcatccgaa tcccaaatgg aacggggcca
     2941 caatgcgcga ctccagcgat tacctggtta acacgttttt tgcgacggtc cgcaaaaaac
     3001 tgaaccgcga cggcctgcgc tggtatggca tccgcacggc ggagcctcac catgacggca
     3061 ccgtgcactg gcatatgatg gtctttgctc atccggaaga aatcgacacc attgtgtccc
     3121 acacccgcga tattgccatt caggaagatc gtcacgagct gggcgatgac ataactccgc
     3181 gctttaaggc ggagtatgta gacggctcaa aaggcacgcc aaccagctac atcgccacct
     3241 acatcgggaa aaacctggac agccgcgccg tggatggcat cgacccgaaa acgggcaagc
     3301 cacgcgttga ccacgaaacc ggaaaatcaa tggccgagag cgtggaacgc gccatcggct
     3361 gggcgcgcct tcaccgggtc cgccagttcc agttctttgg catcccctcc cgccaggtat
     3421 ggcgtgaact gcgccgcctt gccagccaga tggcacgcaa cccggaaggc ccgcaacggc
     3481 tgaaggatga cgcaatggat gcggttcttg ccgccgctga tgccggatgt tttgccacct
     3541 acatagagaa acagggcggc gtacttgttc cacgcaaaga ctacctgatt cgcaccgcct
     3601 acgacctcgc cgatgagctg aacgattacg gcgaacagag cgtacagatt tacgggatct
     3661 ggtcaccact catcggggaa tcctcccgtg tgtgcacgca tccggataac tggaagctgg
     3721 taagacgcaa accggaagcg gaagacagcg cccgcgaaaa tggttttgac cttcagggcg
     3781 gccctgccgc cccttggact cgtggcaata actgtccccg tgtacaggaa acggacaaca
     3841 acgggacaga acagccggaa gaacggccag caccgtggcc gcagctccct gacggcgttg
     3901 atgtggatga atggatgcgc tcactgaaac ggcacgaacg ccgggcgctg atgcgttcgc
     3961 ttcgtgacaa acaggcaaaa aacagcagtg atgaaatgca gagctggaca cagagccgca
     4021 aacagcagcg gcctttgcct gataaccacg aattactcgc taaagaatgg cgggagtctg
     4081 ctgaatctct cggcctgcat atcggtgaac aacagatgca gcacctgtta cggggcggca
     4141 gtctgtacgt tgacgacagc atcattgcac cgcagggatt tgaaattgta cgcaaaccgg
     4201 atacccgccc ggacagccga atcacgcagc tctggcagcg cctgagccgt aatcatggcg
     4261 taagcagcac ggagatccgc cataacccgg tcgccagcta tctgacacag ctgggggcat
     4321 cagaccctga agccgccgca cgcctggcat ccacacttca gcaggaccag aacaccatga
     4381 aaacacccgt taccgtgctt tctgacatgc tgcgcgccat ccgcgacgca gagcacgcac
     4441 agagaatcag tgaaaccact gaacgcgccc gccgcaaagc agacctgctg cggggtggcc
     4501 tgaccagtgg aaacaaaaaa cagacagaaa cgggactcac gaatcccgta aatgagcaaa
     4561 aaacgcgcag cgatatatga agcgcgcaca aaacaggcaa aaacgggatt tcagaatccc
     4621 gtaaacgatt aattaatcaa cataaggaaa agcgacatga aaatttgtat cgacgacggc
     4681 tccaccaaca tcaagctggc atggactgag aacggcgaac gccgcaacgc catcagcccg
     4741 aacagcttca agtcggaatg gtctgcgccg ttcggtggca tgcagcccgc gaactacatg
     4801 cttgatggcg tgcgctatgg ttttgatccg gtcagcgatc gctttgtcca gacaaccgac
     4861 acgcaatacc agtacagcga tgtaaatgtc attgccattc atcacgcgct ggtcaaatca
     4921 ggcatcacgc cacaggaggt ggatgtggtt gtcaccctgc cactgagcga gtatttcgac
     4981 acaaacgcac agccggacat ggccaacatc aaccgcaaaa aagcgaacgt tatgcgcccg
     5041 gtggagtacc agaacggcga agcattcact atccgtaacg tgcgggttat gcctgaatcc
     5101 attccggctg gctttaaggc actggctgac atgagtccgt ttgaatccct gctgattgtg
     5161 gatttgggcg gaaccacgct ggatgtggca aaggttcagg ggcaactggc aggtatcagc
     5221 caggtgtttt gcgatccaca cgtaggcgtt tctctgatgg ccgatgccgt actgtcggtg
     5281 atggccacta acggtatgcg caccagtcac cacatcgcca ataccattat cgaacatcgc
     5341 catgatgaag cctggctgcg ccagcacatc cacaatgacg cgcattacgc cagcctgatg
     5401 gcggttattc gtgaaaagga agaaacactg aaacaacgcg tgatccgcgc gctggcgggt
     5461 ttttcgggtt acgggcgggt gatggttgtc ggtggagggg cggagattgt ggcacccgct
     5521 atccgcgaag cctgcggagt taatgcgact ttcatcgcgg acggggtgcc acagtttgct
     5581 ctggttaatg ggctgtacgc aatggacaag gagtaaacca atgacgacac caaccagacg
     5641 gataagtttc tatctgaagc ctgcggccgt caagaacgaa agcgaagcat gcgcctggct
     5701 gggcagcctt acaccagaag cccgcaaaag cggccaacgc gtggcttttc tggccgggct
     5761 ggcacttctg aaaatgaatc cagcagaggc ttaccgactg gctgcatggg ctgacgatga
     5821 ggcgttatcg gtgacacaaa ccaggacaga acgccccgtg ccacagccag taccaaccgc
     5881 acagataacc agtcagatag cagggaatat ccgggcatta tttcctgaat gacgaatttg
     5941 acggctttta attttatcgg ggtgcactgt cgccccgcac gaggaaaaca cgatgaaaaa
     6001 atacgaatac atgctaatga aagaagcact gcgggctggt ctggctccct gtcctgtatc
     6061 cgtagaagct gttgttggga acacgatttt ccgaacgctg cgaaatcgcg tatccgtgaa
     6121 gatggttcac caaccaccca aatctaccag catgatctat ctgccgctgc ccgaggctat
     6181 caaacttatg cgttcgctga aatttgcgat ttggatgcag acacaaatca acaaagcggt
     6241 gcaaaacacc ttcgttaaga gactaatacc ggagcttgct gccagatgtg caaaatgtac
     6301 cagacccgca cttcaatttg atctgtcatt gattcaacaa gctacggagg cagaactgta
     6361 ccgcctgtca cctatccgtg atgctcacac ggaagcatca gaaatgttcc ggctcatgtt
     6421 tcaatttaat tttgccgttc gacgcacacg cgttcaggtc aatcgcatga tgcaggagca
     6481 ggaagagtta acacagtaca aagggggcag ccagtgagta attcagcacg actacagctt
     6541 ggtttttcac cgctatcaaa aaccatcgta ttggcaaaaa tgcgtgactt aggagacgga
     6601 acaaaacgcc gcgtaggaaa tgatcgcggt cgcgatgtaa ccaacgaggc cgctcaactc
     6661 gtttggcatc tagtcatggc agaaggcggt gaaattaatt gggagctgga tgatggttct
     6721 cgtatgattt tgagggccga gaagagtaaa tagctcaagg gggattaatg ctaaatgatg
     6781 atgaagaaga gctgcttatg caggaatggt cgctcggtga ttatgataat ggtgaaaatg
     6841 ggtgcccgca ttgtggccgt tatcgtttat gtatctgcca gaatggaaaa cacagatgtg
     6901 aaaagtgcaa ttggtctccg gaacttaatg actacgtgcc gatcgagtgg tagaggtaag
     6961 tcacactagg tgttttttag ttgattttat aaatgcacaa tagtgcacaa atttgcacaa
     7021 tttttttgaa cgactttttg cccttccggc ccgcgtggcg gctggatccg tcaaggatcc
     7081 gtgcatgcac aaaaaaacgc gttttttctg cgcgcaggtg acgggggaac agcccgcgtt
     7141 tcagggggta aatagcattc cctgaacgat gtcgcagaga tacaacagaa tggctgtatt
     7201 tctcacgctg agcgtgaaaa agacgtgagg gcttttgatt tgatggggtg aaaggtaagg
     7261 ccgtcaaaat cgcactgaga cggcgagaat atgcagtcaa cgaggtggga ttgcgtaaga
     7321 gtctgactgt cgatgatggc aatcagcagg aaagcgtcgt gaaattatct gactgataca
     7381 ggagctggag agtcggggca taaatttttt atgccccggc gaagcagcag acaagcgaag
     7441 cgcgtcagga tgtgggctgg gtgtctaaca gtgcgtaagg gttaaagcgg atcacctctt
     7501 cgccaagcca gtcattgatg tgcttcatgg cctccatgac gggcatcagc tcgttaattg
     7561 cgtaaacccg cgcggccttc tccacatcac caaacgcact tttttcgccc ggcatcgccc
     7621 ccatcagttg cggcggaacg cggtgcgcag ccagcacatc atcacgggat gccgccttaa
     7681 cattcatgaa ctcatccttt gcggtgatct gctggaacgg caaaatttgc accccctctt
     7741 tgcccccgtt gggcgcatga atgagcacgt ttttaaacgc accaccacca cgtgccccct
     7801 gtagcgtttc tttcagggag tccatgcttt cgcggtttac ctgcgctgca ccgatgtaga
     7861 tgatgcaccc ggcgtgggat ccgttgtcgt agtacagttt tctgaacatg tccgccgaat
     7921 gagaaaggct ggccgagagt aatgcgccaa gatattccgg catgccgtag atttcctggt
     7981 taatatccgg attcatcagg tggcacactt tgccaggacg aaactgaaac gcatccttgc
     8041 catcctgcac ataccaccat gattcaagat cgcttccgcg tcgcatgtat ttcgccaggg
     8101 cgtgccgtaa tttaagcggt tcgccgagca tattgctccg aagctcaagg aatgcgttac
     8161 cgaacacaaa ccagtccagc gccagcgccg agaaatcctg ccgggaaagc agcgggtgcg
     8221 gaatatagca gccgagcaat acattgcgct taaagtaaag cgcagactga tgccaggacg
     8281 tttgccgggc agctcttgcc agaccgtacc agtccaccgg ggtttcatac caccgcccgt
     8341 tatcagcaca gtacatattg tccagcaggt catgcccggt caggcggtaa ggaccatcaa
     8401 atgtgaatgc actgagcgac gattctttcc tgagcgcatc agcgagatca atgcgtgaac
     8461 tcatgcgcac ttttttattt tttctgctca tcagaactcc ataaccgtga aacgctcgtt
     8521 ttctccttcg ccgccaatcg gttcgttaat gacagcaagc atggttgccc acgcaaggtc
     8581 gccgtggctg atccccctcg cgcggtccgt ttcgtaagtg ataaaaccgc ccggtgtttt
     8641 caccttacgc acggcgttaa aggccgcaac cagctcgcgt tcggcgcgat cgtattccca
     8701 ccgcccggca cgcattattt gcagcatttt cagtaccagc gaccgttttg atgacagcgt
     8761 gaaggtgtaa gggatggcgg cgggaaaaaa ccgtttcact atctgataga cggcatcccc
     8821 gtgcccgccc gtcgcatcaa tgccgatgtg ttccacgttg tagcgatacg tgaactcttc
     8881 aataactctg gcctgctctt caaattccag cccctgaacg cgtcgcgtct ccaccgttct
     8941 gaaacgacca ccaggaacag ccggaggaac caccacggac acagcgccgc tgtcgccgtt
     9001 accactgctg ccgtttgcgt catacccaat ccataccgga cgattcccca tcgggcgggg
     9061 ggcaaagggt ttccagtctt tccagtcgtc gtatccgtca acaccgcagc caatcaggat
     9121 attcaggtta aatgccgatt ccccttcgcg gacaaactca cacatataga gattgcggaa
     9181 ctcgtcttcg gtgttttcat cacgaatttc atcaatatcg gtgtgtttcc agccgtgatt
     9241 aaccacatct tccagcgtga caatttgccg ccacgtccgg tcagggcaga taagcccgtt
     9301 atgcagcgtt ttccagtcca cagaaaaacg ctggcgttta tgcgcggcct ttttctcgtt
     9361 ccagcggtcg ccgttccagt aggcgtatgc ctcgtgcgtt tcggtggatg gcgtggagaa
     9421 gcaggtgcgc cgcagtccgc tgagggttgc catagcgcca gccaccttgc gcagttcagc
     9481 aaagcgactg acccagaaaa attcatcaaa ataaaaattg cccgtgtaag attgcgccgt
     9541 cgcagcagaa gtgccgagaa aatgcatctc tgcgccgttg gagaggatga ttttatcgcc
     9601 ccctttcagc tccacatcga cttcagccgc ggccttctga ataatgcttt taaactggaa
     9661 cgcctgacga cgcgacgcag acaaaaaaat ctggttacgc tggtaaggtt gtgccacatc
     9721 gtcacgcagc gccatcagca gtgcttcctg tgcaaaatac caggtcgccc caatctgtcg
     9781 ggatttcagg atcatcctgt tacgtatccc ggcttccctg caaagcgtca gggagtcaaa
     9841 ccagccccgc tgatgccact ccagcctgct gatgattttt tcccgcagtg cggcaatctg
     9901 ttccggcgtg aaatgatttt tgagtttttt cgcccggcct ttctttcctg tggccgtcgc
     9961 atccggctgg ccatcatgca gttttttaag ctgccgggtc agcaggtcta tttccttaaa
    10021 gtcaccacct gttttattct gtttttcagt aagctggatg aggcgcgcat cgatggactg
    10081 cgtgacacgc tgcacgggtg gcgtttcatc ccactggtcg cgttttttcc acgcataaat
    10141 cgtgttcggg tttattccca tcagacgtga tatttctgcg ggcggataac cctgccagta
    10201 aagttgccgc gcacgctggc gcacaaaagc gtcctgaatc attgctcccc ctgagtaatt
    10261 acaggaagat tacccgcgcg cgaaaccgtt ctccttaacc ccctgttctg gccgttttct
    10321 tacaacaaaa gccctttgta tcagcctgtt acgctttgcc atcatgactg aagaaccagt
    10381 cagaggggca aaaactatgg ctaatgaaaa aaagacatcc cgcaaaaagt ttcgcgtggc
    10441 tgtctccgga tcaactgttg atggccgtga aatcagtccg gtgcatctgc gtgaagccgc
    10501 cgagaacttc aacccggatg tttacgctgc ccgcgtgaac gttgagcact atctctcgcc
    10561 atgcccgtca agcgaatttt ccgcaatggg cgatgtcacc gcactgagta cggaagacat
    10621 tacggaaggt ccgctggccg gacgtactgc gctgtatgca gaaatcgaac cgaccgagcg
    10681 catgaagcag cttgtcgcgg acggcaagaa aatctattcc agtatcgaac tgcacccgca
    10741 gttctccgtt aacgggcgcg cctatctggt cgggctggcg atgaccgaca ccccggcaag
    10801 cctgggcact gagcgcctga aattcacggc acagcaacgt caggcggtga tgacgttcaa
    10861 cagtatccag ggtgaagcac cgcttatctc cgaagccatc gagtctgaaa tcatcgaaat
    10921 ggcagaacaa cgccaggaag aaggcaccca gtggtttaac cgcgtaatgg ggattattgg
    10981 tcgtggccgc aaagcggatg acgccagttt ctcccgtatt caggaagcgg tggaaagcgt
    11041 cgcaacgtca caggccgaca ttatcgaccg ttttaatgcg ctggaaacac gccatcagca
    11101 ggacagccag aaaatcactt tactgaccac ggagctgaca gcactgaagg aaaaactgcg
    11161 cacgcaggac ggcgatccgc agaaccggtt caccgcaacg ggcgcagcct ccgaccagct
    11221 ggctgacttc tgataagaca aaggagcaaa ttttttatga atctggtgat gtcagatatt
    11281 acccgcaaca agctgggttg ctatatggcg cagcaggcgt cgcttaacaa tatcccggtt
    11341 tctgcactgg tatcgcgatt taccgtggaa cctgcggtgc agcaacgttt tgaaaacgcc
    11401 tcaaaggata gcaccgaact tacaaaaaaa attaacgtga tcggcgtgac cgaccagaaa
    11461 ggcgaaaaaa tcctcctgga caccacagga ccgattgcac gcacaaatac cagttatgac
    11521 ggaacaaaac gccgtaaccc gaataacgtg gttgatctga aaaaccgcaa ataccagtgc
    11581 gaacaggtga actacgacac gtttatttcg tatccgcagc ttgatgcctg gtcggcacac
    11641 agcgattttc agtcccgcat cagcgcacag attgcccgac aggtggcact tgaccgcatc
    11701 atgatcggtt tcaacggcac atctcacgcg gatgagtcca acttcagcac caacaagctg
    11761 cttcaggacg ttaacgtggg gtggctggag cacatcagaa ccaatgccag cgagcgcgta
    11821 atgaatgacg tgacgctgac ttcccgcaac atggacaaca ccgtggcgca cgcgggtaaa
    11881 tacgcgaacc ctgatgcact ggtacaggac gcgcgctcat ctctgctgga tgaatggcac
    11941 aaggaagctg acgacctcgt ggtgattatg gggcgcaacc tgtttaactc gctgcgtctg
    12001 ccagtgctga acagcatcag cggccagaat cccaatgcgg aattacttgc cgggcagctc
    12061 atcctgtcat cgcgcaccat tggcgggctg ggtgtgttcc ttgcaccgtt cttcccggat
    12121 gcaacgatgc tgatcacctc attcaacaac ctgtcgattt actggcagaa
//
LOCUS       DAZOYS010000120        12083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOYS010000120 DAZOYS010000000
VERSION     DAZOYS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.120"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            240..1349
                     /gene="livK"
                     /locus_tag="ACHUN9_002878"
     CDS             240..1349
                     /gene="livK"
                     /locus_tag="ACHUN9_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0696224.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1397..2323
                     /gene="livH"
                     /locus_tag="ACHUN9_002879"
     CDS             1397..2323
                     /gene="livH"
                     /locus_tag="ACHUN9_002879"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0696225.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2320..3597
                     /gene="livM"
                     /locus_tag="ACHUN9_002880"
     CDS             2320..3597
                     /gene="livM"
                     /locus_tag="ACHUN9_002880"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0696226.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3594..4361
                     /gene="livG"
                     /locus_tag="ACHUN9_002881"
     CDS             3594..4361
                     /gene="livG"
                     /locus_tag="ACHUN9_002881"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0696227.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4363..5076
                     /gene="livF"
                     /locus_tag="ACHUN9_002882"
     CDS             4363..5076
                     /gene="livF"
                     /locus_tag="ACHUN9_002882"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0696228.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5360..6424
                     /gene="ugpB"
                     /locus_tag="ACHUN9_002883"
                     /pseudo
     CDS             5360..6424
                     /gene="ugpB"
                     /locus_tag="ACHUN9_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6522..7409
                     /gene="ugpA"
                     /locus_tag="ACHUN9_002884"
     CDS             6522..7409
                     /gene="ugpA"
                     /locus_tag="ACHUN9_002884"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0696229.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7406..8251
                     /gene="ugpE"
                     /locus_tag="ACHUN9_002885"
     CDS             7406..8251
                     /gene="ugpE"
                     /locus_tag="ACHUN9_002885"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0696230.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8253..9323
                     /gene="ugpC"
                     /locus_tag="ACHUN9_002886"
     CDS             8253..9323
                     /gene="ugpC"
                     /locus_tag="ACHUN9_002886"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0696231.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9320..10063
                     /gene="ugpQ"
                     /locus_tag="ACHUN9_002887"
     CDS             9320..10063
                     /gene="ugpQ"
                     /locus_tag="ACHUN9_002887"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0696232.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10050..10490)
                     /locus_tag="ACHUN9_002888"
     CDS             complement(10050..10490)
                     /locus_tag="ACHUN9_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0696233.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10610..>12083
                     /gene="ggt"
                     /locus_tag="ACHUN9_002889"
     CDS             10610..>12083
                     /gene="ggt"
                     /locus_tag="ACHUN9_002889"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0696234.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         2630 a         3097 c         3476 g         2880 t
ORIGIN      
        1 tttgtgccgt tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc
       61 agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcactg ttatcacaaa
      121 atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt
      181 ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta
      241 tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg
      301 ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt
      361 ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg
      421 gaattaaggg cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag
      481 ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt
      541 gttcttcttc tacccaacct gcatcagata tctacgaaga cgaaggtatt ctgatgatct
      601 cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg
      661 ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc
      721 cccagcgcat cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg
      781 tgcaggacgg gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg
      841 gggagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt
      901 actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc
      961 tgaaaactca gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgctg
     1021 gcgatgctgc cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa
     1081 accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct
     1141 ggatcaccta cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg
     1201 agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc
     1261 tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg
     1321 ccgatggttc atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt
     1381 ttagaaaggt taccttatgt ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg
     1441 cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat
     1501 tatcggcatg atcaacttcg cccacggcga ggtttacatg attggtagct atgtctcctt
     1561 tatgatcatc gccgcactga tgatgatggg cattgatacc ggctggctgc tggtggcggc
     1621 gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc
     1681 ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc
     1741 catcttcctg caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag
     1801 tctgtttaac ggtcagtggg tggtggggca tagcgaaaac ttctctgcct ctattaccac
     1861 catgcaggca gtgatctgga ttgttacttt cctcgccatg ctggcgctga cgattttcat
     1921 tcgttattcc cgtatggggc gcgcctgccg tgcctgcgcg gaagatctga aaatggcgag
     1981 cctgcttggc atcaacaccg accgggtgat tgctttgacc tttgttattg gcgcggcgat
     2041 ggcggcagtg gctggcgtac tgctcggtca gttctacggc gtcattaacc cctatatcgg
     2101 ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc
     2161 aggggcgatg attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct
     2221 gagtacggaa tataaagatg tggtctcatt cgccctgctg attctggtgc tgctggtgat
     2281 gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca
     2341 atggcactgc tctctgccgc gatgttcttt gtcctcgctg gcgtctttat gggcgtgcaa
     2401 ctggagctgg atggcaccaa actggtggtc gacagcgctt cggatgtccg ttggcagtgg
     2461 gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgacctgc tttccagaaa
     2521 gggctaaaaa gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg
     2581 aagcagaaac tgttcctcgt ggcgctgctg gtgcttgcgg tgtcgtggcc gtttatggtg
     2641 tcacgtggga cggtggatat cgccactctg accatgatct acattatcct cggtctcggg
     2701 ctgaatgtgg ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg tttttacgcc
     2761 atcggcgctt acacttttgc gctgctcaat cactattacg gtctggggtt ctggacctgt
     2821 ttgccgattg ccggactgat ggcagcggcg gcgggcttcc tgcttggttt tccggtgttg
     2881 cgtctgcgcg gtgattatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata
     2941 ttgctgctca ataacaccga aattaccggc ggcccgaacg gtatcagtca gatcccgaaa
     3001 ccgactttct tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc
     3061 agtaatttct ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg
     3121 gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg
     3181 gggcgtgcgt gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc
     3241 ccgcgtcgta tcaagctgac tgcctttacc atcagtgccg cgtttgccgg ttttgccgga
     3301 acgctgtttg ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg
     3361 gcgtttgtac tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg
     3421 gcggcaattt tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg
     3481 ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc
     3541 atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc
     3601 agccattatt atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca
     3661 acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg
     3721 gaaaaactac ggtttttaac tgtctgaccg gattctacaa acccaccggc ggcaccattt
     3781 tactgcgcaa tcagcacctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg
     3841 tgcgcacctt ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg
     3901 tggcgcagca tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt
     3961 tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt
     4021 tgctggaaca cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg
     4081 agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag
     4141 gtcttaaccc aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc
     4201 acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc
     4261 gaatttatgt ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta
     4321 ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg
     4381 tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg
     4441 catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc
     4501 ttgctcggca cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat
     4561 aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg
     4621 gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt
     4681 tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gttcccacgt
     4741 ctgcatgagc gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg
     4801 gcgattggtc gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctt
     4861 ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag
     4921 gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc
     4981 ggctacgtgc tggaaaacgg ccatgtggtg ctctccgata ctggtgatgc actactggcg
     5041 aatgaagcgg tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc
     5101 aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag
     5161 gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt
     5221 ttacgccgca tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat
     5281 ttttccgctt atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt
     5341 catctttctg acaccttact tgcaggttta tgaagttggc accgccacca tgatggcatc
     5401 gaaagccatt aaaccggtat atgacgtgtt taaagaggca gggattcaat tcgatgagtc
     5461 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
     5521 ccagccattc aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc
     5581 ggggttagac ccggaacagc cgccgaaaac ctggcaggat ctggcggact cttccgcaaa
     5641 actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact
     5701 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
     5761 cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacattg ccatgctcga
     5821 agagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa
     5881 gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg ctaatattcg
     5941 tgagtacgcc aaatttaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga
     6001 tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaaaataa
     6061 agaaacgtat accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc
     6121 cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg
     6181 tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa
     6241 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
     6301 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
     6361 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
     6421 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg
     6481 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
     6541 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
     6601 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gcggcaaagc gtcgatccgt
     6661 ttggtttctc cagccagttt gtcggtctgg ataacttcgt cacgctgttc catgacagct
     6721 actatctaga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
     6781 tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct
     6841 atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga
     6901 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg
     6961 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcggtgt
     7021 ggaagcaaat cagctacaac tttctgttct tctatgccgc gctgcactcc attccccgtt
     7081 cgttgattga agccgcagct attgacggcg cagggccgat tcgccgcttc tttaagattg
     7141 ctctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct
     7201 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
     7261 ctacgctgat ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg
     7321 ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct
     7381 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
     7441 ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt
     7501 cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac
     7561 acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc
     7621 gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat
     7681 taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct
     7741 cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac
     7801 ggtggaagtc atcgccaacc tgaaaatgct cgacagctac gccggtttaa cgctgccgct
     7861 gatggcctcg gcgaccgcca cgtttctgtt ccgccagttc tttatgaccc taccggatga
     7921 attggtggaa gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt
     7981 ttttccgttg tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg
     8041 gaatcagtat ttgtggccgt tgttgattat taccgacgtt gatctcggca ccaccgtggc
     8101 agggatcaaa gggatgatcg ctacaggcga aggcaccacg gaatggaact cggtaatggc
     8161 ggcgatgttg ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt
     8221 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
     8281 ccaaaagctg ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg
     8341 gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg
     8401 ttgccgggct ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgaccg
     8461 aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaattacgcg ctttatccgc
     8521 atatgagcgt agaggagaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
     8581 aaattgccga gcgcgttaaa gaagcggcgc gcattctgga actggacggt ctgcttaagc
     8641 gtcgtccgcg tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg
     8701 tgcgcgatcc ggcggtgttc ttgtttgatg agccgctctc taacctcgat gccaagctgc
     8761 gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct
     8821 acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg
     8881 gtggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
     8941 ttgtggcgag ttttatcggc agcccggcga tgaacctgct gacaggccgc gtgaataatg
     9001 aaggcacgca tttcgaactg gacggcggta tcgtgctacc gctaaacggt ggctaccgtc
     9061 agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatatagcg ctaagctcgc
     9121 aggcagaagg tggtgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
     9181 tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc
     9241 cgacggcagg cagcacgctg tggctgcatt tggcggaaaa tcagctgcat ctttttgatg
     9301 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg
     9361 cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
     9421 tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca
     9481 tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
     9541 ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct
     9601 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga
     9661 aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg
     9721 ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga
     9781 agctgcacaa caggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga
     9841 cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
     9901 actcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac
     9961 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
    10021 cgatgcgatt gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca
    10081 ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctttccgg cagcatatgc
    10141 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg
    10201 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    10261 gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt
    10321 tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc
    10381 tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    10441 gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct
    10501 cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt
    10561 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    10621 gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc
    10681 cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt
    10741 acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga
    10801 tatcctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc
    10861 ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgttaa tccgctcgaa
    10921 aaatggcaat accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga
    10981 tatgttcctc gatgatcagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc
    11041 ttccggcaca ccgggtacgg tagcaggttt ctcgctggcg ctggataaat acggcaccat
    11101 gccgctgaac aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt ttatcgttaa
    11161 cgacgcgctg gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa
    11221 cagtaaagct atcttctgga aagagggcga gccgctgaaa aagggcgaca cgctggtgca
    11281 ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa
    11341 aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa
    11401 agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg
    11461 gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct
    11521 caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca
    11581 aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc
    11641 ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc
    11701 cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc
    11761 gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt
    11821 ggcggtgacc tatacgctga acaccacctt cggtatgggc attgtcgcgg gcgagagcgg
    11881 tattctgctt aataatcaga tggatgattt ctccgccaaa ccgggcgtac cgaacattta
    11941 cgggctggtg ggcggtgatg ccaacgccgt cgggccgaat aaacgtccgc tgtcatcgat
    12001 gtcaccaacc attgtggtga aagacggtaa aacttggctg gttaccggta gtcctggcgg
    12061 gagtcggatt attaccaccg tgc
//
LOCUS       DAZOYS010000121        12011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOYS010000121 DAZOYS010000000
VERSION     DAZOYS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.121"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            215..985
                     /locus_tag="ACHUN9_002890"
     CDS             215..985
                     /locus_tag="ACHUN9_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0696235.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            976..1449
                     /locus_tag="ACHUN9_002891"
     CDS             976..1449
                     /locus_tag="ACHUN9_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0696236.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHUN9_002892"
     CDS             1557..2096
                     /gene="dnaT"
                     /locus_tag="ACHUN9_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0696237.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHUN9_002893"
     CDS             2099..2836
                     /gene="dnaC"
                     /locus_tag="ACHUN9_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0696238.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHUN9_002894"
     CDS             2885..3379
                     /gene="yjjA"
                     /locus_tag="ACHUN9_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0696239.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3633..5924
                     /gene="opgB"
                     /locus_tag="ACHUN9_002895"
     CDS             3633..5924
                     /gene="opgB"
                     /locus_tag="ACHUN9_002895"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0696240.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHUN9_002896"
     CDS             complement(6063..7085)
                     /gene="lgoD"
                     /locus_tag="ACHUN9_002896"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0696241.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7224..8138
                     /locus_tag="ACHUN9_002897"
     CDS             7224..8138
                     /locus_tag="ACHUN9_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0696242.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8353..9714
                     /locus_tag="ACHUN9_002898"
     CDS             8353..9714
                     /locus_tag="ACHUN9_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0696243.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9763..11418)
                     /gene="tsr"
                     /locus_tag="ACHUN9_002899"
                     /pseudo
     CDS             complement(9763..11418)
                     /gene="tsr"
                     /locus_tag="ACHUN9_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11537..11983)
                     /gene="hpaR"
                     /locus_tag="ACHUN9_002900"
     CDS             complement(11537..11983)
                     /gene="hpaR"
                     /locus_tag="ACHUN9_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0696244.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2911 a         3183 c         2939 g         2978 t
ORIGIN      
        1 gccaaacaat aaattcatta tagtgataat attcatgtga tagtttgttt gcggcgagag
       61 ataattcgct ttttatcacc gagcttgtcc cgataaccag gattaatcag cgcaattttg
      121 atcttaacct accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa
      181 caatacgcag cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt
      241 aacacggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt
      301 ggttgatgag ctttcctcac gactgggtcg ggcgttgggg atggacagcg tcgaaagttc
      361 tatctcttcg aacgccatag tgctgacaac cattaaagat gggcaatgcc tgacatcgac
      421 acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt
      481 gattctggcg gagcaccatc tgctggatta caaaggcgta gagaaacgat ttagccaaat
      541 tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg
      601 tttctgcaaa ctcaataaag gtggctggga tggtgccgtc atcaccttct ttgccagtac
      661 ggccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccgc agatcaactt
      721 ttgcctcacc gctttcgccg ccaccaccat ttccggattg cttttgcaac ttcccacttt
      781 cagcaataca cccactatcg cgatggccgc cagcgttctg ctgctggtgc cgggatttcc
      841 gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg
      901 ggcgatcgcc agtctgctga cactggctac ctgcgtcggc gtggtgatgg cactgacgat
      961 ttgggggcta cgcggatggg tgtgatcgag tttctgtttg cattggcgca ggatatgatc
     1021 ctcgccgcca ttccagcggt cggctttgcg atggtgttca acgttcccgt tcgggcatta
     1081 cgctggtgtg cgctgcttgg cgcgattggt catggttcac gaatgatctt aatgaccagc
     1141 gggctgaata ttgagtggtc aacctttatg gcctccatgc tggtcggcac cattggtatt
     1201 caatggtcgc gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct
     1261 atgttcccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta
     1321 ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt
     1381 gttggtgcgt tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct
     1441 cgcgtataaa attgcctcgc ttaaagcaca cggatgagag acagcctcct ctcctccgtg
     1501 tgttactata aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt
     1561 cttccagagt tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa
     1621 ccgttctggc aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt
     1681 tttatgccgt cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc
     1741 gtccgggaag cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg
     1801 ttgctgtacc catggggaaa ttcgccatgt atccggactg gcaacccgat gccgatttta
     1861 tccgcctggc ggcgctatgg ggtgtggcac taagagagcc ggtgaccacc gaagaactgg
     1921 cctcattcat tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac
     1981 aaaaactggc gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag
     2041 atgtgaatac ggtcagcgaa cctgacagtc aaattccacc aggattcaga gggtaacgat
     2101 gaaaaacgtt ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc
     2161 tgcattcaaa acgggtgaag aactactggc ctggcagaaa gaacaaggag cgatccgctc
     2221 cgccgctctc gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg
     2281 tattcggcca ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca
     2341 gatgaatgcg ttaagcaaag cgcgccagta cgtcgaagag tttgacggca acatcgccag
     2401 cttcatcttt tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa
     2461 cgagctgctg ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc
     2521 gatgaaagat accttcagga atagcggtac cagtgaagaa caactgctta acgatctgag
     2581 caacgttgat ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa
     2641 agtgatcatc aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct
     2701 gaccaacagt aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat
     2761 gcgcctgggt aacagtttgt gggtgatctt caactgggat agctaccgta gccgggtaac
     2821 aggtaaagag tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt
     2881 aatgatgatg aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat
     2941 ttccaccggg gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact
     3001 tggcaaccaa aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt
     3061 gcttagcagc gggaaccagg ccttaagcgc agataacatg aacaacgccg caggcattct
     3121 gcaatactgc gcgaagcaaa agctggcgtc ggtaaccgat gccgaaaaca tcaagaacca
     3181 ggtgctggaa aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga
     3241 tggcattcag ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg
     3301 aacgactccg ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg
     3361 gctgaacttc atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg
     3421 gctctctcct gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga
     3481 ccgcgatcaa aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac
     3541 tagaattgca gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg
     3601 gctaaacggc ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt
     3661 ttctcgcctc tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag
     3721 ccacattaac ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact
     3781 attttactgg cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg
     3841 gtgctggcgt cagcaaatac atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg
     3901 tgttcggtgc gctgggctgg atcctgcgcc gtcgtcgcca tcatccgcac cattttggtt
     3961 acagcctgct ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc
     4021 agataacgga actggtgaaa tcccagtcac gcgatggcga cccggacttt gcggcttatt
     4081 ataaagagcc gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg
     4141 aaagtctcga gcggacctat tttgataacg aagctttccc ggatctcacg cctgaactgg
     4201 gcgcgttgaa aaatgaaggc ctggatttca gccacacgca gcagctgcca ggaacggatt
     4261 acacgattgc gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag
     4321 gcaacgcctc cgcctctgtc tccagcttct tcccgcagaa catctgtctg ggcgatatcc
     4381 tgaaaaactc gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta
     4441 aagatgtgtt cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa
     4501 gcgtggtggc cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg
     4561 atgaagcgtg gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta
     4621 ccctgacagt cgatacccat cacccggatg gttttatctc gcgtacctgt aaccgcaaaa
     4681 aatatgattt tgacggtaag ccgaaccagt cattcagcgc ggtaagttgc agccaggaga
     4741 atatcgcggc gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg
     4801 tcgtctcttc tgaccattta gcgatgaaca acacggcgtg gaaatacctc aataagcagg
     4861 atcgcaataa cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag
     4921 tgaagcgtaa cacgatggat aacggcgcga cggtgctgga cattctcggt ggcgataact
     4981 atctcggact tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc ttcctcaata
     5041 tcaaagagaa aacattggcg tggaagccgg atatcatccg cctgtggaaa ttccctaaag
     5101 agatgaaaga gttcaccatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt
     5161 tccgtttgcc gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg
     5221 aatactcagc accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct
     5281 gggttgaccg ttgctataag atggcacaac tctgggctcc ggaactggca ctctccaccg
     5341 actggtgtgt ctcgcaaggg cagcttggcg gtcagcaaat tgttcagcat gttgacaaaa
     5401 caacatggca gggcaaaacg gcatttaaag atacggtcat cgacatggcg cgttataaag
     5461 gcaatgtcga tacgctgaaa attgttgata acgatattcg ctacaaagcc gacagtttca
     5521 tcttcaacgt cgccggtgcg ccagaagagg tgaaacagtt tagtgggatt tcccgtccag
     5581 agtcgtgggg ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc
     5641 atccgctgcc aaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca
     5701 gccgtcctat tccggtacgc gtaggcaatg aagaacaaac ccttgtgctg ggcaatgaag
     5761 tgaccaccac cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc
     5821 cgccggaacc tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg
     5881 ggatcggcat ggtggaaatt aaagtggtgg aacgtgaagg ataattcctg actgatgtgt
     5941 aggtcgcata aggtgcgacc tacacacctt ctgcggccta caaaacatca ttgagaaaca
     6001 gaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat aatctcgcct
     6061 tatcagaaag taattacacc cttaatcaac tcacgattgt taatcacatc gcgctcgtag
     6121 gtttctgcca gcgtggcgaa cggatagcga tgggttaaca tcatgtcagc agtgattttt
     6181 ccttccgcca tcagtcgacc gactttagca aaatcttccg gcgtggcgtt gcggctaccc
     6241 atcatcgtcg tttctttttt atggaattcc ggatcggaga actgcaactc acctttaaac
     6301 aggccgacaa ataccaccgt gccgccgtga cgaatcaaat tcacggtgtt attcatcgca
     6361 tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt
     6421 agctgcgcgt caaaatcttc ggctgacggg tccagtaaag gtaattccag acgcgttgcc
     6481 acatgttcac ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc atcggcttta
     6541 gcgattgccg ccgcgcccag accgattggc cccgccccga ccaccagcac ctgctcgccg
     6601 ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc aatcaatgct
     6661 gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac
     6721 tcactaaaac cgccatcctg atgcacgcca atgactgaaa ttttttcaca gcaattggta
     6781 cgcccgcttt tacacgccgg gcattgctga caggcaacat aagggatcac agcaacttgc
     6841 tgaccatttt taagatcagc aatattttta cccagcccaa caatctcccc acatatttca
     6901 tggcctaaaa cacgtggata actaaaaaat ggttgatttc caccccaggc atgaatatcg
     6961 gtaccgcaaa tcccgacaga cttaattttt attaatgctt cattgtcacc cggaataggt
     7021 atctcgcgtt gtttccagac taattctttc ggctgctggc aaattaaaac attcatcgta
     7081 gacatgattc ggctctccat tttttgttgc cttagatatc aatgaaaatg aaaaatggca
     7141 gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt
     7201 aatggactca caaggaggaa aatatgagtc gttcacaaaa tttacgccac aatgtgatta
     7261 accaggtcat tgatgatatg gcgcggggtc atatcccttc cccacttcca tcgcaaagtg
     7321 cgctggcgga aatgtacaac atcagccgca ccacagtgcg tcatattctc agccacttac
     7381 gcgaatgcgg cgtcttgacg caggttggaa acgactatgt tattgcgcgt aaacctgacc
     7441 atgatgacgg tttcgcctgt actaccgctt caatgagtga acaaaacaaa gtgtttgagc
     7501 aggctttttt cacgatgata aaccagcgcc agttacgccc aggggaaacg ttttctgaac
     7561 tgcaactggc tcgggccgcg ggtgtcagcc cggttgtagt aagagaatat cttttaaaat
     7621 tcggccgtta taatcttatt cacagcgaaa agcgcggcca gtggagcatg aagcaattcg
     7681 atcaatccta cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc
     7741 agcactttct caacctgcct gatcatgatc cccgctggct gcaggcgaaa accatgctcg
     7801 aacgtcatcg gctactgcgt gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg
     7861 acagagattt tcactcctta ctgctttcag ctgccgacaa tatctttttt gatcaatcgc
     7921 ttgagatcat ctcagtcatc tttcattttc actatcaatg ggacgaaagc gatcttaaac
     7981 agcgcaatat cattgccgtc gatgagcata tgaccattct cagcgctctg atttgccgca
     8041 gtgaccttga tgccaccctt gcgctccgta accatttgaa ttcagctaaa caatcaatga
     8101 ttcgatccat taatgagaac acgcgttatg ctcattaagt acggattaaa aacagcaaac
     8161 tattagcggg tgcacggttt tatcaaaaga taaataccgg gaaattcccg cttacctatg
     8221 ctcacaatca gtcgactaaa aatgtttttg tgagcggtag taaagtccta aaactttaac
     8281 ctgccgttaa tgtcaacctt gcgtaattca caattacgta aacaattaaa attataaata
     8341 caggagtccg gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc atttactcca
     8401 tcttcgtcgg cagatattcc cgtgccacca gatggattag ttcaacgcag tacccgaatt
     8461 aaacgcattc aaaccaccgc catgttgtta ttattttttg cggcggtaat caattatctc
     8521 gaccgcagtt cgctgtcggt agcaaattta acgattcgtg aagaattggg attaagtgcc
     8581 accgaaatcg gcgctttgct ctccgtgttt tcactcgctt acgggattgc gcaacttcct
     8641 tgcggcccac tattggatcg taaaggccca cgcctgatgc tgggactggg gatgttcttc
     8701 tggtcactgt tccaggcaat gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg
     8761 cgtatcggta tggggattgg tgaagcgccg atgaacccat gcggtgtaaa agtcattaac
     8821 gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc
     8881 attggcgttg ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc
     8941 gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc
     9001 tatcgcaacc gcgagcacgt agaactgact gccgttgaac aagcttatct caatgcaggt
     9061 agcgtcaatg cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac
     9121 cgtacaatgt ggggaatgat gctcggattc agtggcatca actacactgc gtggctgtat
     9181 ctggcctggc ttcctggtta cctgcaaaca gcctataacc tggatttaaa aagcacaggg
     9241 ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt
     9301 actgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt
     9361 gccgggatgt tctgttctgc cgcctttacg ctggtcgtac cgcaagcgac aacatccatg
     9421 acagcggttc tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc
     9481 tggggcttga tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag
     9541 aactttgcca gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat
     9601 accacccact cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca
     9661 ctggcgtaca tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct
     9721 tatgcccgat aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt
     9781 ctcctcgcta tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac
     9841 agccgatgtt tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg
     9901 actggcctgc tcttccagcg cggcggcggc agcggcagac tcttccacca gcgcggcgtt
     9961 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
    10021 ctgctcatca gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacggcgct
    10081 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
    10141 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tgcgctgggc
    10201 cagattacgc acttctcccg cgaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc
    10261 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
    10321 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
    10381 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct
    10441 ggcctggcgc gcattttcgg cgttctgctt caccgttgcg gtcagttact ccatgctggc
    10501 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
    10561 ggtggcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
    10621 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
    10681 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
    10741 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
    10801 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
    10861 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
    10921 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
    10981 gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
    11041 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
    11101 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
    11161 ggttgaaccg ctaccaatat tgttctgatc catcatgtag cggatacccg cgcggttgag
    11221 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
    11281 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
    11341 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat
    11401 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
    11461 aaagcaaaaa ctttatgaaa aggtgtaacc caccatccat ggaaaaacca ggcgctggtt
    11521 atcagatact aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca
    11581 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
    11641 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
    11701 cctctttggt tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
    11761 ctaaaccgtc gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg
    11821 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
    11881 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
    11941 cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa
    12001 attcaaaatc a
//
LOCUS       DAZOYS010000122        12005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOYS010000122 DAZOYS010000000
VERSION     DAZOYS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.122"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            381..1769
                     /gene="narU"
                     /locus_tag="ACHUN9_002901"
     CDS             381..1769
                     /gene="narU"
                     /locus_tag="ACHUN9_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0696245.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1851..5591
                     /gene="narZ"
                     /locus_tag="ACHUN9_002902"
     CDS             1851..5591
                     /gene="narZ"
                     /locus_tag="ACHUN9_002902"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0696246.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5588..7132
                     /gene="narH"
                     /locus_tag="ACHUN9_002903"
     CDS             5588..7132
                     /gene="narH"
                     /locus_tag="ACHUN9_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0696247.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7132..7827
                     /gene="narW"
                     /locus_tag="ACHUN9_002904"
     CDS             7132..7827
                     /gene="narW"
                     /locus_tag="ACHUN9_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0696248.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7824..8504
                     /gene="narI"
                     /locus_tag="ACHUN9_002905"
     CDS             7824..8504
                     /gene="narI"
                     /locus_tag="ACHUN9_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0696249.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8583..9476
                     /locus_tag="ACHUN9_002906"
     CDS             8583..9476
                     /locus_tag="ACHUN9_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0696250.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9572..10417)
                     /gene="nhoA"
                     /locus_tag="ACHUN9_002907"
     CDS             complement(9572..10417)
                     /gene="nhoA"
                     /locus_tag="ACHUN9_002907"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0696251.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10590..11159
                     /locus_tag="ACHUN9_002908"
     CDS             10590..11159
                     /locus_tag="ACHUN9_002908"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0696252.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11163..11390)
                     /gene="pptA"
                     /locus_tag="ACHUN9_002909"
     CDS             complement(11163..11390)
                     /gene="pptA"
                     /locus_tag="ACHUN9_002909"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0696253.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11490..>12005)
                     /locus_tag="ACHUN9_002910"
     CDS             complement(11490..>12005)
                     /locus_tag="ACHUN9_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0696254.1"
                     /translation="EKFPLKELNNPENDSYAISEKSHGREEIRLHIVCDVPDELIDFT
                     FEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMGRNY
                     LASVLTGSGLS"
BASE COUNT         2817 a         3100 c         3130 g         2958 t
ORIGIN      
        1 agaggaaccg atagaccttc tggacagatc gttttcctcc agttcatggc ctttttagtg
       61 aataaatcat cactaattct ataagtgtct ggtcttgttg ataatatagt taatatgtaa
      121 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaaccccgt
      181 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      241 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      301 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      361 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      421 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      481 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      541 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
      601 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
      661 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
      721 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
      781 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
      841 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
      901 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
      961 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1021 catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1081 atatcgccag ttcacgcgcc tcaattgacg accagctccc tgtcttacaa cgcctgcatc
     1141 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1201 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1261 ttggcccatt tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1321 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1381 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1441 ggctgtttct gactgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1501 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1561 aagaagccat caccgaaacg gcggcggctc tgggctttat ctcagccatt ggcgcagtgg
     1621 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg
     1681 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     1741 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc
     1801 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     1861 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     1921 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     1981 aaatcgtgcg ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg
     2041 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2101 accagcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatctct
     2161 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgtg
     2221 aagctctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc
     2281 aaaagtgctt aagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2341 aggaacttaa ccaactgatt gccgccgcta acgtctggac catcaaaatc tatggtccgg
     2401 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtgtcatac gctgccggaa
     2461 cgcgttatct gtcgctgtta ggcggcacct gtttaagctt ctacgactgg tattgcgacc
     2521 tgccgcccgc ctcgccgatg acctggggcg aacaaaccga cgtgccggaa tctgccgact
     2581 ggtataactc cagttacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg
     2641 acgcccactt ctttaccgaa gtacgttaca aaggcaccaa aactatcgcc attacccctg
     2701 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     2761 gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca
     2821 gcgactactt tatcaactac tgccgccgct atagcgacat gccgatgctg gtaatgctgg
     2881 aacctcgcga cgatggtagc tacgtacccg ggcgcatgat ccgcgcgtct gacctggtag
     2941 atggactagg cgaaagcaat aatccgcagt ggaaaaccgt ggcggttaat accgcaggtg
     3001 aattggtagt gccgaatggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3061 tggaatccat tgccgccggt aaggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3121 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3181 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3241 atggcaacac ctgtccggtg gtcagcgttt atgatttggt gctggcgaat tacggcctcg
     3301 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3361 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3421 aatttgccga taccgcccat aaaacgcatg gacgctcaat gattatcctc ggcgctggcg
     3481 tcaaccactg gtatcacatg gatatgaact accgtgggat gatcaatatg ctgatcttct
     3541 gcggttgtgt cgggcaaagc ggcggcggct gggcgcacta tgtcggtcag gaaaaactgc
     3601 gcccacaaac cggctggttg ccgctggcct ttgcgctcga ctggaaccgc ccaccgcgcc
     3661 agatgaacag tacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     3721 ctgctcagga gttactttca ccgcttgccg atgccagtaa gtacagcggt catctgattg
     3781 atttcaacgt tcgcgccgaa cgcatgggct ggctaccttc tgcgccgcag ctggggcgta
     3841 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     3901 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     3961 atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc
     4021 acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4081 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4141 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4201 tcgttctgcc caccgccacc tggtacgaaa aagacgatat gaacacctcg gatatgcatc
     4261 cgtttattca tccgctttct gcggcggtcg atccggcgtg ggaatcacgc agcgactggg
     4321 aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag
     4381 aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt
     4441 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga
     4501 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4561 cattgatgga caaacttggc aatggtggta aagggatttc gtggaatacc caggatgaaa
     4621 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     4681 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     4741 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     4801 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     4861 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgtc tcctataatg
     4921 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     4981 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5041 ctatcgacac ccgtagcgtc agtgagatgc gccagatccc gccaaacggc ttcccggaaa
     5101 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5161 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaaccgatg
     5221 cccgagaact gaccattgtc gataacgact gggtggaagt ctttaacgcc aacggcgcgc
     5281 tgacggctcg tgcggtggtc agccaacgtg taccgccggg tatgaccatg atgtatcacg
     5341 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5401 ataactcggt cacccgtgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5461 tggcctgggg ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga
     5521 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     5581 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgaca aatgtatcgg
     5641 ttgccatacc tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta
     5701 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     5761 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatac gcccgcgtct
     5821 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagattga
     5881 tgattactac gaacctttca ccttcgacta cgaacatttg catagcgcac cggaaggcaa
     5941 acatatccct actgctcgcc cgcgttcgct gattgacggt aagcggatgg acaaagtgat
     6001 ctgggggcca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     6061 caacttcgag gccatgcaaa aggagatgta cgggcagttt aaaaacacct tcatgatgta
     6121 cctgccgcgc ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct gcccaagcgg
     6181 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     6241 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     6301 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     6361 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     6421 cattgaggaa gcggcgagca ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt
     6481 gttcctcgat ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag gtattccaca
     6541 aaacgtgatt gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg actggaaact
     6601 ggcgctaccg ctgcaccctg aatatcgcac cctgccaatg gtctggtacg ttcctccgct
     6661 gtcgccgatt cagtcctacg cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc
     6721 cgccatcgaa agcctgcgta ttccggtgca atatctcgcc aatatgttga gtgccggcga
     6781 taccggtccg gtactgcggg cgctgaaacg gatgatggcg atgcgccact atatgcgttc
     6841 acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc
     6901 ccaggtcgaa gaaatgtatc gctatctcgc cattgccaac tatgaagatc gttttgttat
     6961 cccgaccagc catcgggaaa tggcgggcga tgccttcgca gaacgcaacg gctgcggttt
     7021 tacctttggc gacggttgcc acggttcgga cagtaaattc aacctgttca acagtagccg
     7081 tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc
     7141 ctcaaagtga tcggcctgtt gatggagtat ccggatgagc tgttgtggga atgcaaggag
     7201 gacgcgctgg cgttgatccg ccgcgacgcg ccgatgctta cggatttcac ccacaacctg
     7261 cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg aagtgtttga ccgcgggcgc
     7321 accacgtcgc tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg cggccaggca
     7381 atggtggacc tgctggcgga gtatgaaaag gtcggcctgc agctggattg tcgggaactg
     7441 cccgattatc taccgctgta tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa
     7501 gaaggattgc tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc
     7561 gaggcaccgt ggtacgcgtt gtttgatgct ctgctgcaac tggcgggaag tatactttca
     7621 agtgacagtg tcacaaaaca agtaaacagc gaagagcgcg atgacacccg ccaggcgctt
     7681 gatgcagtgt gggaagagga acaggtgaag tttattgaag ataacgccac ggcgtgtgac
     7741 agctcgccgc ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc gcagtatgtc
     7801 gacatcagtg cgggaggtgg gaaatgattc agtatctgaa cgtctttttt tacgatatct
     7861 acccgtacat ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac gactacgggc
     7921 agtacacctg gcgcgcctcc tcaagtcaaa tgctcgataa acgcgggatg gtgatatggt
     7981 cgaatctgtt ccatatcggc attttgggga ttttcttcgg gcacttgttc ggcatgttaa
     8041 cgccgcactg gatgtacgcg tggtttttgc cagtggcagc gaaacaactc atggcgatgg
     8101 tgctcggcgg tatttgcggt gtgttgacgt tgattggcgg cgcaggactg ctgtggcgca
     8161 gtctgacaaa tcagcgcgtg cgtgccactt ccactacgcc ggatatcatc atcatgagca
     8221 ttttgctgat ccagtgtctg ttggggctaa gtaccatacc gttttcagca cagtatcctg
     8281 atggtagcga aatgatgaag ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg
     8341 gatcgtcaga gatgctcaac ggcgtagcgt tcgtcttccg cctgcatctg gtgttgggaa
     8401 tgaccatctt cctgctcttc ccgttcaccc gactggtgca tgtgtggagc gcgccgtttg
     8461 agtactttac tcgtcgatat caaattgtgc gttcgcggcg ataatttcac cctggccgga
     8521 tagcccttcg ctctccggcc acttgcaggc tatgatagtt ctccagtcac aggagaacgt
     8581 taatgaaacc gcaggtgtac cacgtcgatg cttttacctc tcaaccattt cgcggcaatt
     8641 ctgccggtgt tgttttcccc gccgataatc ttagcgaagc acaaatgcag cttatcgccc
     8701 gcgagttagg ccattcggaa actgcttttc tgctgcacag cgacgacagc gatgtgcgca
     8761 tccgctactt tacgccaacg gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc
     8821 actatgtacg tgcgaaggtg ttaggtttag gaaattgcac gatctggcaa acatcgctgg
     8881 caggaaaaca tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt tcgctggaac
     8941 aaggtacgcc gggctttgaa ctgccgctgg agggtgaagt acgcgcggcg attatcaacg
     9001 cattgcatct tactgaagac gatattctgc cgggtttgcc gatccaggtc gcgacgaccg
     9061 ggcattctaa agtgatgatc ccattgaagc cggaagtgga tatcgacgct ctttcgcctg
     9121 accttaatgc gctgaccgct atcagtaaac agattggctg caatggtttc ttcccgttcc
     9181 agatccgtcc aggcaaaaac gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg
     9241 tggaagatcc ggtgactggc aatgccaatg gcccgatggg tgcatggttg gtacatcaca
     9301 acgtattgcc ccacgatggc aacgtgttgt gtgttaaagg ccatcagggg cgcgcattgg
     9361 ggcgtgacgg catgattgaa gtgacggtga caattcgtga taaccaaccg gaaaaagtca
     9421 ccatttcagg cacagcggtg attttgtttc atgccgaatg ggcaattgaa atctgaaatc
     9481 gttgcctgat gcgcttcgcc tgtcaggtct acaaccgggc taatgtatgc cggataaggc
     9541 gtttacgccg catccggcaa cctgacataa attattttcc cgcctccgga tgcgtatcaa
     9601 acgccgccat caccagcgct aactcatcca cagtaaagcc atgtttcgca tcatccacgc
     9661 ccagaccaaa ctgttcctgc atcacggcat ataacgacgc cacatccggt agatttcgct
     9721 gctccaccgc gtgcccattt tcataatggg taaaatgaaa attggtcagt gtcagcttgc
     9781 cgccgtccgg caaatggcgg cacatcagca aatgatggcg aaaatgcgac tgcggccagt
     9841 gcgccgacca gaaattgccc atcacataat cgctttgttg ctgctcgcag agatcgaagc
     9901 ggtacatcgg ctgccaatgc tgatgatgat taaactgcaa cacccaatca tcaccctcct
     9961 gcaacaaccg atactctccg tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg
    10021 gcgcggttag cgtctgccca ccgaaaccga catcagcaat ccatttttcc ccttccagtt
    10081 ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat aacactacgc
    10141 gccctaacaa gctgcgaacg ttaaacccca gctcgcgtaa cacccgctca aacacgccat
    10201 tctgctcaaa acagtaaccg ccacgacggg ctatcaccag tttctcttcc agcgattgat
    10261 catcaagctg tatttccctc ggcagcaaaa cgtcaaggtt ttcaaacgga atggtgcaat
    10321 tgtgtttcag gtgcaatgca cgaagcgtat caatattgac cgcagcagct cccgaccagt
    10381 taatacgggc aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt
    10441 ctcaacttat aacctggaat agacgccgca ccggctattt tgtgcgatcg cggacatgca
    10501 tcacgtttgc aaatcgccaa tcccgttaat ttcgttactt attgcgttac ggactacgct
    10561 taaaaaaacc ttcttcgcga ggcgatacca tgagccgatt catccccata gaattacacc
    10621 atgccagccg tctgttaaat catggcccaa ctgtcttgat caccagtttc gatgagcaat
    10681 cccagcggcg taacattatg gctgcagcct ggtcaatgcc ggtggagttt gaacctccac
    10741 gcgtggcaat tgtggtagat aaatcgacat ggaccaggga gttgattgaa cgtaacggta
    10801 agtttggcat cgttatcccg ggcgttgcag caactaactg gacgtgggcg gtgggaagtg
    10861 tgtcggtgcg tgaagaagat aaatttaatt gctatggcat tccggtggtg aaaggtccgg
    10921 tgcttggttt gccacttgtt gaagagaaat gcctggcgtg gatggagtgc cgcctgctac
    10981 ctgcgacttc tgcgcaacaa aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg
    11041 cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt
    11101 tgcatcattt aggtgctggc acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag
    11161 cattacatgc tgtaaccggg tttctttatc aaagcctcca tttggggctc gatttcggca
    11221 tcccagatag cttgccaaga ttctggttga atctgctgta gagcaatgct tatcgagcta
    11281 tctttactgt tcagatggcg aataataacg tcggtaatat ctgcagcaag tgctgctttt
    11341 tgttgttcgt ccagttcacg cggaaagcat ttaatgtcga tgtgcggcat gattacgctc
    11401 cttttgaaag atgtcagtaa aattaacaca cattgtgaat gtcatgtgtc cggtaaagcg
    11461 tttatacgac aataatgccc agggcaagat tacgaaagcc cgctccccgt aaggactgac
    11521 gccaggtagt ttctgcccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat
    11581 accttatcat tcgtcaaaat attaatagca atgtgccgta tccctgaaaa taattctgct
    11641 gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgccaatgc
    11701 agcttattct ccacgtgcca gtggtttcgg attgctgtgg cgaacttctc tgcggttaaa
    11761 tcagcagaac tgatataata tctgaccgtc atttctggct ctttcttttg ttctgctatt
    11821 atggaccgaa aggagactgc cacgcataat ttcttcagcc ctttccattc aaacgtgaaa
    11881 tcaataagtt catcagggac atcgcaaaca atatgaagac ggatttcttc tctgccgtga
    11941 ctcttttcac taattgcgta actgtcattc tctggattat ttaattcttt cagcggaaat
    12001 ttttc
//
LOCUS       DAZOYS010000123        11909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOYS010000123 DAZOYS010000000
VERSION     DAZOYS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.123"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUN9_002911"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUN9_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0696255.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUN9_002912"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUN9_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0696256.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUN9_002913"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUN9_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0696257.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUN9_002914"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUN9_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0696258.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     TDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHUN9_002915"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHUN9_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUN9_002916"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUN9_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0696259.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHUN9_002917"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHUN9_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUN9_002918"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUN9_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0696260.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHUN9_002919"
     CDS             7629..8642
                     /locus_tag="ACHUN9_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0696261.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHUN9_002920"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHUN9_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHUN9_002921"
     CDS             9533..10327
                     /locus_tag="ACHUN9_002921"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0696262.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHUN9_002922"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHUN9_002922"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0696263.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2875 a         2893 c         3197 g         2944 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcggtgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcc
//
LOCUS       DAZOYS010000124        11811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOYS010000124 DAZOYS010000000
VERSION     DAZOYS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.124"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHUN9_002923"
     CDS             <1..488
                     /locus_tag="ACHUN9_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0696264.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUN9_002924"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUN9_002924"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0696265.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUN9_002925"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUN9_002925"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0696266.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUN9_002926"
     CDS             complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUN9_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0696267.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUN9_002927"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUN9_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0696268.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUN9_002928"
     CDS             complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUN9_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0696269.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHUN9_002929"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHUN9_002929"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0696270.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHUN9_002930"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHUN9_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0696271.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="ACHUN9_002931"
     CDS             complement(6839..7747)
                     /locus_tag="ACHUN9_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0696272.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="ACHUN9_002932"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="ACHUN9_002932"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0696273.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="ACHUN9_002933"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="ACHUN9_002933"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0696274.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11811
                     /locus_tag="ACHUN9_002934"
     CDS             10715..>11811
                     /locus_tag="ACHUN9_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0696275.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMG"
BASE COUNT         3005 a         3001 c         2930 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg g
//
LOCUS       DAZOYS010000125        11798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOYS010000125 DAZOYS010000000
VERSION     DAZOYS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.125"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            27..593
                     /locus_tag="ACHUN9_002935"
     CDS             27..593
                     /locus_tag="ACHUN9_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0696276.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            749..2781
                     /locus_tag="ACHUN9_002936"
                     /pseudo
     CDS             749..2781
                     /locus_tag="ACHUN9_002936"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2758..3102
                     /locus_tag="ACHUN9_002937"
     CDS             2758..3102
                     /locus_tag="ACHUN9_002937"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0696277.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3117..3848
                     /locus_tag="ACHUN9_002938"
     CDS             3117..3848
                     /locus_tag="ACHUN9_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0696278.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3845..4906
                     /locus_tag="ACHUN9_002939"
     CDS             3845..4906
                     /locus_tag="ACHUN9_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0696279.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5058..6104
                     /locus_tag="ACHUN9_002940"
     CDS             5058..6104
                     /locus_tag="ACHUN9_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0696280.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHUN9_002941"
     CDS             complement(6101..6892)
                     /gene="nei"
                     /locus_tag="ACHUN9_002941"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0696281.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHUN9_002942"
     CDS             complement(6928..7662)
                     /gene="pxpA"
                     /locus_tag="ACHUN9_002942"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0696282.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHUN9_002943"
     CDS             complement(7652..8584)
                     /gene="pxpC"
                     /locus_tag="ACHUN9_002943"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0696283.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHUN9_002944"
     CDS             complement(8578..9234)
                     /gene="pxpB"
                     /locus_tag="ACHUN9_002944"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0696284.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHUN9_002945"
     CDS             complement(9257..10000)
                     /gene="ybgI"
                     /locus_tag="ACHUN9_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0696285.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHUN9_002946"
     CDS             10271..11752
                     /gene="dtpD"
                     /locus_tag="ACHUN9_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0696286.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2819 a         3155 c         2895 g         2929 t
ORIGIN      
        1 tttcttttct gttaaaggac tcagttatgt ttaaaggaca aaaaacattg gccgcactgg
       61 ccgtatctct gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc
      121 actttaaagg tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag
      181 aggttgaact cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg
      241 ttgctgtcga cctgcgctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg
      301 gcaagatttc taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc
      361 cgattctcaa caacaccagc acgggtgaag ccaccggcgt cggcgtacgt ttaatgaata
      421 aagatcaaag caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct
      481 ccagcgaaca aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg
      541 taacgccagg cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac
      601 tttggcggcg ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat
      661 ttatcgactc tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcggtcga
      721 attcaacctt aatgttctcg ataaatcgat gcgcgaccgc attgatattt cattattaaa
      781 ggaaaaagga gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca
      841 aatcagtaac gggcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat
      901 caatgattta ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cgttaccatt
      961 gataaatcag tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc
     1021 caatcagcaa ctgaatatta gtattccgca agcctggctg gcgtggcact cagaaaactg
     1081 gaccccaccc tccacatgga aagaaggtgt cgccggtatc ctgatggatt acaacttgtt
     1141 tgccagcagc taccgcccac aggacggcag cagcagcact aacctgaacg cctacggtac
     1201 caccggaatt aacgccgggg catggcgctt acgtagtgat tatcagttga atcagactga
     1261 tagcgatgat aaccatgaac agtcaggcgg aatatcgcgc acctatcttt ttcgtccatt
     1321 accgcaatta ggctctaaat taaccctcgg cgaaacggat tttagttcca atattttcga
     1381 cggtttttct tataccggcg cggcactggc aagtgacgag cgaatgttgc catgggaact
     1441 acgcggctac gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag
     1501 tcaatcaggc cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga
     1561 ccttaatcag tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt
     1621 gaacaatttc caggtttcgg cagcatcgac gcccttcctg actcgtcagg gacaggttcg
     1681 ctataaactg gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac
     1741 cttttttagc aatgaagttt cctgggggat gctgtcaaac acctcgctgt acggcggcct
     1801 gctgctttct ggtgatgact accattctgc cgcaatgggt attgggcaaa atatgctgtg
     1861 gcttggtgcg ctgtcgtttg atgtcacgtg ggccagtagc cattttgata ctcagcagga
     1921 cgagcggggc ttaagctacc gttttaatta cagcaaacaa gtggatgcca ctaacagcac
     1981 gatttccctc gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct
     2041 ggatcacaaa tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttattgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat
     2281 cacgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga
     2461 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     4021 tgacgatatt aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     4081 gcatgtttat gcctgggtaa ccccctaccc gggggtgcag gatcgctatt atcaactcgg
     4141 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     4201 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     4261 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct
     4321 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     4381 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac
     4441 tgcccaaaac ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag
     4501 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     4561 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     4621 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4681 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4741 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4801 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4861 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4921 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4981 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     5041 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     5101 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     5161 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     5221 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     5281 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     5341 gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct
     5401 ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag
     5461 atattcgcct ggttgcattt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     5521 ttctggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     5581 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta
     5641 cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg
     5701 cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg
     5761 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5821 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg
     5881 gtatggcgtg ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca
     5941 ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     6001 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     6061 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagccagggc
     6121 accagtaaaa cgggcgagat gacagcgtgg ttttctcaat gatgctgcca cagcgttcgc
     6181 acggttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     6241 catccacctg ccccctcgta gcgtaggaaa atcgaggaat atccagtaac gcgtgtgcga
     6301 gtgcatccag ttgcgccgca ttaagatctt tcgctttatg atttccagtc aacccaacct
     6361 gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca
     6421 gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     6481 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     6541 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     6601 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6661 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     6721 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6781 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6841 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6901 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     6961 tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac
     7021 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     7081 catctctagc gtttgcgcca acgcctgttc ttcgttttca atcaacgcgc ctcgctgact
     7141 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     7201 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat
     7261 caatgccgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     7321 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     7381 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     7441 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     7501 aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatgattt gcgcatcgcc
     7561 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     7621 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacacta
     7681 attgttcgaa ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7741 ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa
     7801 tgatacaggc aatacgcggg taaccatcgg tggtctgtgc gtcgttcatc aacacaatcg
     7861 gctgcccgtt atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc
     7921 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     7981 agctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     8041 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     8101 cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     8161 ggccttccag cccgccaatc cccactttga gatcggtgct gcatgacccc attaccggcg
     8221 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     8281 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     8341 ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa
     8401 ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca
     8461 ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgatt cccgactggc
     8521 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     8581 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     8641 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     8701 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8761 aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8821 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8881 ttcaacaacc tgtttttcgc taaacccgca atgcgccgcg accaccgcca aatccggtcc
     8941 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     9001 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     9061 acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     9121 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     9181 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     9241 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     9301 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     9361 ctgcagcata aaaatgcaac ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     9421 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     9481 cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg
     9541 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     9601 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9661 ccgccagctg tgcgttattg cccagctcag gatgtacgtc aagcggcaaa tgccagccat
     9721 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9781 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9841 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9901 tctctttgcc ttctacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9961 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
    10021 gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac
    10081 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
    10141 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
    10201 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg cagtcaattg
    10261 tggaattatt atgaataaac acgcatcaca accgcgcact atttactatg tcgttgcgct
    10321 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
    10381 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
    10441 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
    10501 ggcggtgatg ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga
    10561 gatccatccg tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt
    10621 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10681 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc
    10741 ctgtggttac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10801 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10861 cgttaataaa aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct
    10921 ggttctgctg gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt
    10981 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    11041 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    11101 tatgttgttc tgggccttcg cacaacaggg cggcagctca attagccttt atatcgaccg
    11161 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    11221 tgccttcgca gttatgctat gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    11281 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc
    11341 cggattctgc attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct
    11401 gccactgatg gtgttaggcc tcgcggtgat gggctttgcg gaactgttta tcgacccggt
    11461 tgccatgtcg caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat
    11521 ctacatgctg ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac
    11581 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    11641 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11701 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag
    11761 caccgatgcc tgatgcgacg cttgacgcgt cttatcag
//
LOCUS       DAZOYS010000126        11780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOYS010000126 DAZOYS010000000
VERSION     DAZOYS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.126"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..613)
                     /locus_tag="ACHUN9_002947"
     CDS             complement(<1..613)
                     /locus_tag="ACHUN9_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0696287.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRF"
     gene            complement(736..2382)
                     /gene="fumB"
                     /locus_tag="ACHUN9_002948"
     CDS             complement(736..2382)
                     /gene="fumB"
                     /locus_tag="ACHUN9_002948"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0696288.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2460..3800)
                     /gene="dcuB"
                     /locus_tag="ACHUN9_002949"
     CDS             complement(2460..3800)
                     /gene="dcuB"
                     /locus_tag="ACHUN9_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0696289.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4371..5090)
                     /gene="dcuR"
                     /locus_tag="ACHUN9_002950"
     CDS             complement(4371..5090)
                     /gene="dcuR"
                     /locus_tag="ACHUN9_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0696290.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5087..6718)
                     /locus_tag="ACHUN9_002951"
     CDS             complement(5087..6718)
                     /locus_tag="ACHUN9_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0696291.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6899..7129
                     /gene="yjdI"
                     /locus_tag="ACHUN9_002952"
     CDS             6899..7129
                     /gene="yjdI"
                     /locus_tag="ACHUN9_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0696292.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7141..7413
                     /locus_tag="ACHUN9_002953"
     CDS             7141..7413
                     /locus_tag="ACHUN9_002953"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0696293.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7640..7936
                     /gene="ghoS"
                     /locus_tag="ACHUN9_002954"
     CDS             7640..7936
                     /gene="ghoS"
                     /locus_tag="ACHUN9_002954"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0696294.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7964..8137
                     /gene="ghoT"
                     /locus_tag="ACHUN9_002955"
     CDS             7964..8137
                     /gene="ghoT"
                     /locus_tag="ACHUN9_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0696295.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8256..9773)
                     /gene="lysS"
                     /locus_tag="ACHUN9_002956"
     CDS             complement(8256..9773)
                     /gene="lysS"
                     /locus_tag="ACHUN9_002956"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0696296.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10010..11467)
                     /gene="dtpC"
                     /locus_tag="ACHUN9_002957"
     CDS             complement(10010..11467)
                     /gene="dtpC"
                     /locus_tag="ACHUN9_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0696297.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11526..>11780)
                     /locus_tag="ACHUN9_002958"
     CDS             complement(11526..>11780)
                     /locus_tag="ACHUN9_002958"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0696298.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2893 g         2985 t
ORIGIN      
        1 caaagcgccg tagctggcgg caatgaaagc gagcgaggaa tatcaccgtc gttaatgcgc
       61 caagcagcgc gcagaacata tcggattgcg tgtcccactg gtcgccctgg gtgccgagaa
      121 aatcatccgc tccctggccc attgccagcg ccgcccacca ctcgattaat tcatacatcg
      181 cgcttatcgc cagcgccacg cagcagacta ggaacgccac cattttacgt ccgcgcacgt
      241 acatcccgcg cacgagaatt tctcgtgcca ccagtgcagg caccagcccc tggaaaaaat
      301 gccccagctt gtcatacgga ttacggctca accccagcca ttcctgcacc tcaaaaccga
      361 tggggacttt cgcgtaggtg tattgcccgc cgaccatcag gatgatggcg tgaaagaaaa
      421 tgagcgtata gagcagcggc gttaacggat aacgtttagc ggtggcaagc agtagcggca
      481 cgacaataat caccggtgtc acttccatca gccaggtgag tttgtcatgg gccgaaatgc
      541 cggtataaat caggattaac gttagcgcca gtgcgccggt gttaagaatt aacggtttga
      601 gtgtgcgggt catggtgagt tcgcaagtca gggaaaatac tgactattca ccagcaagca
      661 tgaaattaca atcgcaaaag aggtggccca gggggatcac ctggcagcat gctgccaggc
      721 gctgggccga agaggttact tagtgcagtt cgcgcactgt ttgttgacga tttgctggaa
      781 gaagtcgtta cctttgtcat cgaccaggat aaacgccggg aaatcttcta cttcgatttt
      841 ccagatagct tccataccca gctccggata agcgacgcat tccagatgct tgatgctctg
      901 ctgcgccagt accgccgccg gaccgccgat gctaccgagg tagaagccgc cgtgtttatg
      961 acacgcgtcg gtaacctgct ggctgcggtt gcctttcgcc agcatgatca tgctgccgcc
     1021 gtgggattgc agcagatcca cataggagtc catacgacct gcggtggttg ggccaagtga
     1081 acctgatgga taaccggcag gggttttcgc cggacccgcg tagtagatcg ggtgatcttt
     1141 gatgtactgc ggaagttctt taccggcgtc aatcagctct ttcagcttgg cgtgtgcaat
     1201 atctcgaccc acaataatgg tgccagtgag cgacaaacga gtggaaaccg gatattgcga
     1261 aagctgggcg aggatctctt tcatcgggcg gttaaggtcg accttcaccg cttcgccttc
     1321 accggcctga cgcagttctt gtggaatgta ctgacctgga ttgtgttcca gtttttcgat
     1381 ccagatacct tcgcggttga ttttcgcttt aatgttacgg tcagcggagc aggagacgcc
     1441 catgcctacc gggcaggatg cgccgtgacg tggcagacgg ataacgcgaa tgtcgtgcgc
     1501 gaagtattta ccgccaaact gcgcgccaag accgagtttc tgggcctctt ccagcagttc
     1561 ctgttccagc tggacatcgc ggaacgcctg accatgttcg ttcccttccg tcggcagttc
     1621 atcgtaatag tgagcgcttg ctaatttgac ggttttcagg ttggtttccg cagacgtacc
     1681 gccaatcaca aacgcgatat ggtacggcgg gcaggctgca gtaccgaggg tacgcatttt
     1741 ctcgacgagg aagtttttca gtttgccggg agtcagcagg gctttggttt cctggtagag
     1801 atacgttttg ttggcagagc cgccgccttt cgcaacgcaa aggaatttgt actcatcgcc
     1861 atctaccgcg tacaggtcga tttgcgcagg caggttagtg ccggtgttga cctctttgta
     1921 catgtccagc gccgcgtttt gcgagtagcg caggttatct tcgatatagg tgttatagac
     1981 gcctttcgac agcgcttctt catcaccgcc accggtccac acgcgctggc cttttttacc
     2041 gacgatgatc gcggtgccgg tatcctggca ggtcggcagc acgcctttgg cggcgatttc
     2101 ggagtttctt aagaattgca gtgccacgta cttgtcgttt tcgctggctt ctggatcgtg
     2161 aagaatagcc gcaacctgtt tctggtgtgc cggacggagc atgaaagaag cgtcgtgaaa
     2221 ggcttgctgc gccagcaggg tcagggcttc tggttccact ttcaggatgg tttcgccgtc
     2281 gaagtcggca acgctaacgt aatcggaagt gagtagatag tattcggtat tgtctttccc
     2341 catcgggaaa ggtgcctggt agataaaggg tttgtttgac atagcttcca gcctgtaact
     2401 ctgtatttgt tattcgaaaa tggcgtgccg cgtagtcacg ggcggcacgc aaagtgcatt
     2461 tataagaacc cgtacatcgc ggcgaagatc cagccgaaga cgcacgatac gctcacacca
     2521 atcaaccccg gcagaataaa gctgtggttg atgacgaagc gaccgatgtg ggtggtgccg
     2581 gaacggtcaa actgaatcgc tgccagatcg ctcggataag tcggcaggat gtaataaccg
     2641 tagcaagccg gtgctgaagc cacgatgtat gccggatcaa cgccgatcgc cagcgcgacc
     2701 ggaacaatcg ccgccagcgc cgcagcctga gagtttacaa acttggaaac cagcagcaga
     2761 acaatggcat aggcccacgg atactctttc accatttcac ccagtacgcc ctgaatttca
     2821 gacatatgcg caccgaacat ggtttctgcc atccatgcga taccgtacac cgccacgatg
     2881 gcgatcatac cggaacggaa gacttcgttt tttgagatag acgcgggatt ggttttggtc
     2941 aggataataa tcagcgcccc ggtcagcagc ataaacatct gaataaccag taccatcgac
     3001 agcggtttgc cgccgaagga tggacgcagg tccgaatcag caccaagaag ggcgactaca
     3061 gcgattgccc cgaggaaaat ccacattgcc agccagttgc ttttcggcag ttttttgtcc
     3121 agcagcgtcg cggtatcacc gtaaacatac tcacggtttt ccggtacgga gatgaatttc
     3181 tggaactctt cgtctttatc cagatcttta ccacggaacc agctgaagat accgatcgcc
     3241 aggataccga ttaacgtcga tggaatggtg attgccagca ggtcgaggaa ctcaagatgg
     3301 cgaccatcaa aggtgacatt acccagcatt gcaaccagag acacgaccgc aaccgacacc
     3361 ggactggcga tgatccccat ctgtgcaccg atagaacttg ccgccatcgg acgttccgga
     3421 cggatgttgt tcttaatggc gacgtcgtag atgatcggca gaatggtgta aaccacatga
     3481 cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg gcgcgacaat tgagacatat
     3541 ttcgggttgc ggcgcagcag cttctcggca atttgcagca tgacatcaag accgcccgaa
     3601 gcttgcaagg tcgccgatgc cgccaccacc gcaatgataa ccagcatgac atcaaccggt
     3661 ggtttacctg gctgaaggtg gaagacgaag accagaatga ccagaccgat accgcctaat
     3721 aaacccagcg cgataccacc ctttctggca ccataaaaca gacatatcag tattatgata
     3781 agttggatag taaataacat gtgtgaaccc tcgcgataat cctatttaaa tttttgctga
     3841 atagatcaca gtcacgttct gttttgtatg aactgttttc agagcctgac taaatatccg
     3901 tttggtcgtt accggcttta gcaaatacct cacagtgaat attggctgat taaagcagcg
     3961 gcaaatttat gcactgtgat aagcggcttt ttcagagaga aagactctct ggcgatgggt
     4021 ttatagtttg tactgcgcag atgttaatgt tttgtttgct gttatcttat tgatatttat
     4081 cggtctattt tttgttctca gggcagatgt cattaggttt atagattaat ctgatctacc
     4141 catttgtggg taaaaataca cataatgcgg gtgacataat agttaattaa cttttgttag
     4201 cgttttgaaa ttaaaaacac cgttcacctg aagagatatt aatttttagc gatgatgcag
     4261 ggataattat atttgatctg gcacaagttt tactgatgaa ggatgtaact tgtgccaggg
     4321 gtattttgca ttacggtaat aattattact tacagataac cagcgatccg ttattggcaa
     4381 tattgtttca gtagtgagta gtgttctgcc tgaatacggt aacggtaaac tggacgcccc
     4441 gtgacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca gatgaggtat
     4501 ttacggcagg aaacacgcga aatgttaacc tcgttggcta gctcgtcggt tgaaaattca
     4561 tagtcctgat gcgcgtcaat ccactggcac agtgtgcgta acgtctgcgg cgttaagcct
     4621 tttggcaagc gacgaggatc ctgttcgttg gagctgctgc cgtggattag ctgatcaagc
     4681 tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg ccagtcggtg
     4741 agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac gacaccgtaa
     4801 tgcagcgaat ctttaatggt tgccgcatcg gctgcggagg agatgacaat cacatcactt
     4861 ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt ttgcatatag
     4921 atatcgagca atatcaggtc gataggcgta tcgctattga agataatctc tttggctttc
     4981 tccagcgtcg aggctgttcc acagcattga aagcctggga tttgtgctac gtatcggcga
     5041 ttcagctccg cgaccattgc gtcgtcatcg ataattaata cattgatcat ctgttcgacc
     5101 tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt tccgattcca
     5161 cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa ccgacgcctc
     5221 gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg tcgggtgcga
     5281 tccccggtcc atcatcgtta acttcgcagt gcagccagcc gtgacggtag tgcaatgtta
     5341 cgctaatttc gccaccgggt tccggcccta atgcttccag cgcgttttct atcagatttc
     5401 ccaatgtggt aatcagcgtc gcgacctggt cctcactgcc gctgtctggc agctggcttt
     5461 cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg attaatcttg ctgattaaaa
     5521 aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct tcctgatagt
     5581 tattggctgt ttttagaatg taatcttcca actgcttata actcttcaga tgcaataatc
     5641 cgagaatcac gtgcaattta ttcataaatt cgtgggatcg ttcacgaagt gcgtcggcat
     5701 agttgaccag accgtcgagt cgctgcatca gtttacgtac ttcggttttg tccctgaagg
     5761 ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg atcagtaata
     5821 gccggtcttt aatcgtaatc tcttcgtcgc ggcgcggcgt accgtcgcgt aacacttccg
     5881 agacatctac cacctgtgac catgagtggc tgagcgtcga cagtttctca tcgtcctgcg
     5941 acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgacc tcgccgcgat
     6001 cgtccacggc aacgacgcct tctttgatag actgcaacat ggcctggcgt tgctcaaaca
     6061 gcgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacc ttaaccagaa
     6121 tgcaggtgcc aatcagtccg accagcatgc caaataatac cgaccagata atgctccagc
     6181 gactgtcatt gatttgttgg gtcacgcggc ttaactcaag gccgatcgcc accacgccaa
     6241 tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc gccagaaaac
     6301 cgcgattgat agcgacattt tctttgccat tcagcgcttt aaggatgtca tcacctttaa
     6361 atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactttgc atatcggtaa
     6421 cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc tggatgccgc
     6481 tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagttggcg agggtacgcg
     6541 ccactgccag tgccttgttg gctagcccgt ctcgcgtcat atcactgatt tgcgagaagt
     6601 aaatcagatg caccaccaat agcaccgaga acagtaccgc actgaccatt aagatcactg
     6661 tggtactcaa tttcatcgga cgtttgcgta acatgcggta gggcaatgaa tgtctcatca
     6721 gcttccttgt gtgacaaatt tcttaaacat tatctctgat gaggcgggta attcaaaggg
     6781 agtaagaatg attggctata taggggaaga gattctggca acggaaactg ccagtgctgt
     6841 atgaagattc cagggctatg cttatagcga taatcatact gatgagagag ggaaagtcat
     6901 ggatcaggcg ctactggacg agggttatcg ctgttatacc ggcgaaaaga tcgatgtcta
     6961 tttcaacact gcgatatgtc agcattctgg caattgcgta cgtggcaacg gcaagttatt
     7021 taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgtcgcca ctgtggttaa
     7081 agtgattgat acgtgcccga gcggcgcgct gaaataccgt cataaataag cgagggtaaa
     7141 atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg caagcaaatc
     7201 gctgaaattg tctttgtgcc gactggagag aatttagcga ttatcgaaca taccgatgtc
     7261 gatgaaagcc tgaaagggca ggggattggt aaacagctgg ttgcaaaagt cgtggaaaaa
     7321 atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca cgaatttgat
     7381 aaaacgcggg agtatgatga tattcgcagt tgatgggaga gtacagagtc acgatatttt
     7441 tcatgctctc cgcggagtga tgcaggagag catctgaagg gtagggggat gcacaaagaa
     7501 tgggcagaga gcgcgttttt ttgtcccaag tcatcccctt tactgagcaa aaaaaagaat
     7561 atctcctata tgagaatcat caatcggggt tatcaagttt tgcgtcccca gagcattcaa
     7621 tattgatagg agtcatatta tggaaggtaa aaacaagttc aatacttatg ttgtttcttt
     7681 tgattatcca tcatcttatt cctcagtgtt cttaaggtta agatcattga tgtatgatat
     7741 gaatttctct tctatcgtgg ctgatgaata tgggatacca cgacaattga atgaaaattc
     7801 cttcgcaata acgacatcgt tagccgcaag tgaaatcgaa gatttaatca ggcttaaatg
     7861 cttagactta ccggatattg attttgacct caacattatg acagttgatg actatttccg
     7921 tcagttttac aagtagccag gaaggataag aagaacataa aaaatggcac tattctctaa
     7981 aatattaatt ttttatgtga ttggtgtgaa catatccttt gtcattatct ggtttatctc
     8041 acatgagaaa acacatattc gtttacttag tgcattcctg gtcggaataa cctggccaat
     8101 gagtctgcct gtggcattac ttttttctct cttttaggag ctgtagttga gtggacccgc
     8161 tggttatcgt gaaacgaagc gtctctcctc aaaactggat ttgcggaacg acgaacgaag
     8221 cagtcgcctg actgcttcgt tcattaaagt gaaatttatt tctgtgggcg catcgccggg
     8281 aagagaataa cgtcgcgaat agtatgactg tttgtaaaca gcataatcat tcggtcgata
     8341 ccaataccca gaccagcggt tggcggcaga ccatattcca gcgcagttac gtaatcttcg
     8401 tcatagaaca tggcttcgtc gtcacctgca gctttagcgt taacctgttc ctggaaacgt
     8461 tcagcctgat cttctgcgtc gtttaattcg ctaaaaccat taccgatttc acgaccaccg
     8521 atgaagaatt caaaacggtc ggtgatttcc gggttaacat cattacggcg cgccagcggt
     8581 gacacttctg ccggatattc cgtaataaag gttggctgaa tcagatgtgc ttctgccact
     8641 tcatcaaaga tctccgtgat aatacgtccc agcccccagc ttttctctac cgtaattccg
     8701 atagattcag ctaatgcttt agcagcatca aaattatcca ggtcggccat atcggtttct
     8761 ggacgatatt ttttgattgc ttcgcgcatg gtgagttttt caaacggttt gccgaaatca
     8821 aacacatgct cgccataagt gactttagtg gtacccagaa cctcttgtgc cagggtgcgg
     8881 aacagtgact ctgtcagttc aatcaaatcg tggtaatccg catacgccat gtagagttcc
     8941 atcattgtga actcaggatt atggcgaaca gaaatacctt cattacggaa gttacggttg
     9001 atttcgaata cccgttcaaa accgcctaca accagacgtt tcagatacag ctccggcgcg
     9061 atacgcagat acatatctaa atccagagca ttatgatggg taataaacgg gcgagcagat
     9121 gccccacctg gaattacctg catcatcggg gtttctactt ccataaagcc gcgcgcgacc
     9181 atgaactgac ggatagcggc cagaattttt gaacggacaa caaacgtttg acgggattta
     9241 tcgttagcga tgaggtccag ataacgttga cgataacgga cttcctgatc ctgcagacca
     9301 tggaatttat ctggtaaagg acgtagtgct ttagtcagca ggcgcaactc agtacagtga
     9361 atggaaagct cacccgtttg cgtcttaaac agcgtaccgc gggcaccgat aatgtcaccc
     9421 agatcccatt ttttaaactg atcgttataa acaccttctg gcaggctatc tcttgcaacg
     9481 tacagttgaa tacggccacc gacatcctgc aacgttacaa aggaggcttt ccccatgata
     9541 cgacgggtca tcattcggcc agcaaccgag acttcaatgt ttaaggattc cagttcctgg
     9601 ttattcttcg catcaaactc ttcgtgcaac tggtcagagg tatggtcgcg gcgaaaatca
     9661 ttgggaaacg ccacaccttg ctgacgtagt gccgccagtt tttcgcggcg atttctcagt
     9721 tcatcgttaa aatcaatagc ctcattggct ccccgtgttt cttgttcaga cattttggtt
     9781 cctctaaatc cagctttcaa tttagcgtag ataaagagac agatcggtac actgtattat
     9841 ctgcctcgac tattaataac tcagtttatc ttatcgaaac taacgcttac ctcaagttga
     9901 tatcactaat aaataaccat caaaatggtt taaatgactt attaatctcg atttactagt
     9961 gtgattaact gaaagttaat ttattcataa ccaattgttt ttacgacagt taatcgttgc
    10021 tctcctgtat catattcgtc ggcgtgctaa acagaaaacg ggtagcaaag gccaatacga
    10081 cgatgatcgc gacacaagcc aacgtccact ctcccatctg agaaaagaat cgctggtaag
    10141 ctgcaattac cataccgcta atttgcgact ctgtcgtctg ctgtgccacg acgcctgcca
    10201 gccagttggc gaccgcgcct gttgccagca tataaatacc ggttaatacg ccagacattt
    10261 tcagacgcgt gatttgcgca atcgccaccg ggtcaataaa gagttctgca aagcccatta
    10321 gcgccaaccc ggatatcatc acgcccattg acgcttgacc gtcagccgct gcatgtcggg
    10381 catcaaatgc caacaacata aagccacaag ccatcagcag taagccaaag gcaaacttca
    10441 gccagacgcg caatgttgag ttgccgcggc tttctggcga cgccagccag gccagtacaa
    10501 ccccagcgag catcactgca attgcattca ccgactggaa tagtgctgta ggtacttcaa
    10561 tattgaatgc ctgacgattc acaaagcgat cgataaacaa gctgatggta ctgccgccct
    10621 gttgtgccag tacccagaac aacgtcccga caaacatcaa caatacaatt tgccaaagag
    10681 cacggcggtg ttcggggaat ttgatcatca tgcgggcaat gatttgtgcg gcaatgaggc
    10741 aaacgatcgc cagcaaatat ccggaccagt cgttctccag cagcagggta aaaaatactg
    10801 gggctaaaca gagcatcacc actaaccagc tccatactgg taaggcaaat ttgacgctag
    10861 tgagcgcttt tttatccata ctacgcgtgg actggaaatg acgatgaccg cttaagaaaa
    10921 tcaacaaacc gataaacatg ccgccacccg caagggcaaa gccaacatgc catccatacc
    10981 actgagcagc caggccgcag gcgattgggg cggcgataga accgatattg cccgcagcat
    11041 acagcagcga aaaaccgcca tcacgtcgat gatcgttctc gtcgtagagc tcgccaagca
    11101 aacagctgat gtttgatttg aataaaccgt agccacaaat aatgattgcc agcgccagat
    11161 acaggctaaa ggttgaattt gtatcaatgc ccagcaccac atggccaagg gtcattaaca
    11221 gcgcgccggc aatcactgca gtgcggttgc cgagcaggcg gtcggcaagc cagccgccga
    11281 gaataggggt aacgtaaacc agagaagcat atgcgctgaa caggctgatg gcatggttat
    11341 catcaaaacc aagctgatgg gtgagataga gaatgagtaa ggcacgcatg ccgtaaaaac
    11401 tgaagtactc ccagatttgg atcgccacga tatagtatat cgcgcgcggc tgtgagggtg
    11461 ttttcatgtg ttctccttat gagcaaaaaa gggaagtggc aagccacttc ccttgtacga
    11521 gctaattatt ttttgctttc ttctttcaat accttaacgg tatagcggcc atcagcctga
    11581 cggtatgcac cgtgaatatc ggtttcaaat cccggatagt gagcgccgat ttcacacagc
    11641 atctgcagga actccagaac cggacggctt tcttcggtga tcatttcacc cggcattacc
    11701 agaggaactc ccggcggata cggaaggatc atattggcgt taatacgacc taccatttcg
    11761 tcgaggtaaa cttcttcggt
//
LOCUS       DAZOYS010000127        11665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOYS010000127 DAZOYS010000000
VERSION     DAZOYS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.127"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHUN9_002959"
     CDS             <1..400
                     /locus_tag="ACHUN9_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0696299.1"
                     /translation="KIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVK
                     VAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASD
                     QKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            611..934
                     /locus_tag="ACHUN9_002960"
     CDS             611..934
                     /locus_tag="ACHUN9_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0696300.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            931..1761
                     /gene="amiD"
                     /locus_tag="ACHUN9_002961"
     CDS             931..1761
                     /gene="amiD"
                     /locus_tag="ACHUN9_002961"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0696301.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1758..2771)
                     /locus_tag="ACHUN9_002962"
     CDS             complement(1758..2771)
                     /locus_tag="ACHUN9_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0696302.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2870..4300)
                     /locus_tag="ACHUN9_002963"
     CDS             complement(2870..4300)
                     /locus_tag="ACHUN9_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0696303.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4311..5312)
                     /gene="ltaE"
                     /locus_tag="ACHUN9_002964"
     CDS             complement(4311..5312)
                     /gene="ltaE"
                     /locus_tag="ACHUN9_002964"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0696304.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5349..7067)
                     /gene="poxB"
                     /locus_tag="ACHUN9_002965"
     CDS             complement(5349..7067)
                     /gene="poxB"
                     /locus_tag="ACHUN9_002965"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0696305.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7200..8168)
                     /gene="hcr"
                     /locus_tag="ACHUN9_002966"
     CDS             complement(7200..8168)
                     /gene="hcr"
                     /locus_tag="ACHUN9_002966"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0696306.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8180..9832)
                     /gene="hcp"
                     /locus_tag="ACHUN9_002967"
                     /gene_synonym="priS"
     CDS             complement(8180..9832)
                     /gene="hcp"
                     /locus_tag="ACHUN9_002967"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0696307.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9976..10875)
                     /gene="lysO"
                     /locus_tag="ACHUN9_002968"
     CDS             complement(9976..10875)
                     /gene="lysO"
                     /locus_tag="ACHUN9_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0696308.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11370..>11665)
                     /locus_tag="ACHUN9_002969"
     CDS             complement(11370..>11665)
                     /locus_tag="ACHUN9_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR0696309.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2848 a         3079 c         3090 g         2648 t
ORIGIN      
        1 caagataaaa ccatggttgc cagtaaaatg gacaaaagag ttaataaatg attatttgta
       61 tggctgttta ctatctctgt actctatctg tgcaagggat atatataata tgagcgccgg
      121 gaataacgtg aaggttgctg cggatgcttt ccttgaaatt attttctctc ttaagaataa
      181 atattgcatt aaactattaa gcgctcagga acgggcgttt atttacgaat ttgcaagaat
      241 gatcttcgct tatattaatg acaaaagcat tgagatctta ttactttctt gttttgcggc
      301 gtctgaccaa aaagcaatcc agaggtatat acctcagtca caagatgggg aagactttag
      361 gagtcattta caatataagt tgcctctttc cgctcattaa tgttgttggt gcaacataat
      421 aagacaggta agcggtaccc tataatgaaa ccaatatcgg gttatgcaac ggataatcgt
      481 aaagacttaa gtgggtgttt ttgacacaat gagatgattt cctaaactga tcaaaacctt
      541 cttcccaaca tggcatatca gttcgataat gctttagaga tcgaaggctt aacaaacaag
      601 gagatcgatc atgcaatttt ctacaacccc aactctggaa ggccagacca tcgttgaata
      661 ttgcggtgtg gtgaccggcg aagcgatttt aggtgccaat attttccgtg atttctttgc
      721 cggtatccgc gatatcgttg gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc
      781 acgggagatc gcctttgagg aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt
      841 cgtcggtatt gatatcgact acgaaacggt cgggcaaaac ggcagtatgc tgatggttag
      901 cgtcagcggt acggcggtga aaacgcgtcg atgagaagag tcttctggct ggtcgctgcc
      961 gctctgttat tggcagggtg tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag
     1021 cttgataccc gacgccaggc gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac
     1081 tacaccgcag atgattttga tagctcgctg gcgacactga ccgataagca ggtcagctcg
     1141 cattatctgg tccctgcggt accaccgcga tacaacggta aaccgcgcat ctggcaactg
     1201 gtgccggaac aagaactggc ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc
     1261 cttaacgaca cctctattgg cattgagctg gaaaaccgtg gctggcaaaa atcggccgga
     1321 gtgaaatatt ttgccccgtt tgaaccgaca cagattcagg cgcttattcc actggcgaaa
     1381 gatattattg cccgttatca catcaagccg gaaaacgtag tggcacatgc ggatatcgca
     1441 ccgcagcgca aagacgatcc ggggccatta tttccctggc agcaactggc gcagcagggg
     1501 attggtgcct ggccggatgc gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac
     1561 acaccggtag ataccgcgtc attgctggag cttttggcgc gctacggtta tgacgttaaa
     1621 cctgatatga caccgcgcga gcagcggcgc gtgattatgg cattccagat gcatttccgc
     1681 ccgacgttat ataacggcga agcggatgca gaaactcagg cgattgccga agcattgctg
     1741 gagaaatacg ggcaggatta gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt
     1801 ttctcgatac cttcatctag ggtgatgatc ggctgataac ctaactcttc ctgcgcacgc
     1861 gtaatatcca gcgtaaagtc aaaattcaac ttggagacgc cgtagtgggt cagcggcggc
     1921 tcttttgctg acttgcggcc taaacgctcc atgctgcggg cgatcatatc cagcatcggg
     1981 taggggacgg aacgaatacg acagtcaata ttcaactcgt cgatcagctt ctgcacgatg
     2041 ctgcgcagtg tgcgatgctc gccgttggtg atattgtaca cacgcccgga aggtagctta
     2101 tcgcaggctt cctggcttgc cagccacatt gcgtgcacgg tattttcata gtaggtcata
     2161 tcgaccagcg cactgccgcc atgcggtaac agaatactgc cgtagtggtg catcatatgc
     2221 gccagacggg gaataaagac tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata
     2281 gtaaagcgcg tttgtggatt cgcctgcgaa agcatattga tcacttcttc gctggctgct
     2341 ttgctgcggg caaactcgtt ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga
     2401 tggtggtgat aatcgaagta cagggagggg gaagagatat gaataaagtt acgcacaccc
     2461 caggcgacag cccattcacc caggcggcga gtggcgcgaa cgttagccag atcgaaagcc
     2521 tgttgtgtcc cccagggtga ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg
     2581 agcatcactt tagcttgtga tgaaaccagc tcggtcagat ccgccggaac aaactctgcg
     2641 cccatttttt ccagcaattt gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg
     2701 cctttctggc ataaaaactc taccgcgttt cgacctaagc cgctggtggc gccggtaacc
     2761 agtaccttca tatcaatcca ctgttgttga gaaaatgacg tgcgcattct tccgtgattt
     2821 cccccatgat gcaatgggaa acatgaaaga ataacgcagg ttttgtcgat taatctgtgc
     2881 tttgttctgc cagtctggcg atttgttttg ccattccgcg aaaaataaac agatgcgcgg
     2941 ggatcatcaa taaccagtaa aacagccccg gcataccgtg cggatgccag aaagcgcgga
     3001 catcgatagt acgatagtcg cctttatctt ccaggctaaa acacagtcgt cccagccccg
     3061 acgctttcat gccaaataac aacgtaagtt gtttttccgg ttcaacgaca atcactttcc
     3121 agctatccac cgcatcgcca gtctgtaaat attcgcgctc cgggcggcct ttcgccagct
     3181 tatgaccgat cgcgcggtcc atcaacgccc gtgtctgcca caaaatattg ccaaagaaat
     3241 aacgctcttt accgccgatt tggttcacta cctgccataa agcagcaagg ctggcggacg
     3301 ttttaacggt aaaccccgcc tgtttggcaa aataaccgta ctccggtcgc cagcgggcaa
     3361 aggcctgagc gtcgtagccc cagtcgctgg agttgaccag tttttcctcc tctttcaacg
     3421 tgctacgtac cgcgtcatcg aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg
     3481 tatcatccgc cagcagatcg tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg
     3541 gtacggaagt aatcacattg agaaaccaca ccgaaatcca gcgggtgggg agggggatgg
     3601 ggatcaacca gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac
     3661 tgagcacctc tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca
     3721 acgccaccag atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc
     3781 agcgtggcgg cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg
     3841 ctgaacctgc gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac
     3901 gaagaatgtc cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg
     3961 cctgcaacga actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga
     4021 cgttgagagc cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca
     4081 ccagaaaata gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga
     4141 gatcgacttt atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac
     4201 gtgccgccgc caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa
     4261 tgtagccact ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc
     4321 aggaatgcgc gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc
     4381 gtcaccaggc ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg
     4441 cctaacgcgg cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc
     4501 atcacatccg cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct
     4561 tcctgcaacc gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg
     4621 gactgacgca tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca
     4681 cgattaccga cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg
     4741 aacgaatcac aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta
     4801 aagatgcgcg caccgtcaac atgcagcgcc agattgcgct tgcgggtaaa ttcccatgct
     4861 tctttcaggt attcacgcgg cagcactttg ccgttgtggg tgttttctag actgagtaat
     4921 ttggtgcggg cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt
     4981 agcgtgccgt cggcagccgc gtcgatgggt tgcggttgaa tactgcccag caccgctgcg
     5041 ccaccggctt caaacagata attatgcgcg gcctgaccga caatatactc ttcgccgcgt
     5101 tcgcagtgac tgagcagagc gaccaggtta gcctgggtgc cggtcggcag aaaaatggcg
     5161 gcttctttac cggaaagctc tgcggcgtag tcctgcagag cattaacggt agggtcgtct
     5221 ccgtaaacgt cgtccccaac cggggcggcc atcattgctt cgagcatggc gcggctcggt
     5281 cgggtaacgg tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc
     5341 cacccttttt accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg
     5401 ctgatgattg cgcgcaacat atacaagcta aatcctttgg cctgttcgag tttgatctgc
     5461 ggtggaatgg ctaactcttc tttggcgacc accacatcca ccagcaccgg accgtcgatg
     5521 gagaaggcgc gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta
     5581 atgccgcacg cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc
     5641 aaatagccac cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg
     5701 acaattttca ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta
     5761 aaaccgccat cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc
     5821 agcgcctgcg gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc
     5881 ttgccgttca tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg
     5941 aaaatagcgt catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg
     6001 gctttctcgc tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc
     6061 agcgctttat ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc
     6121 agagtcgact tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg
     6181 gctgggttga tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga
     6241 aattgcgtgc cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag
     6301 aagccgatta acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta
     6361 ccgcgcaggg catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta
     6421 tgcgcccccg cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt
     6481 tgcgccagtt tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac
     6541 cagtgcgtgg ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca
     6601 accgaaacgc cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc
     6661 tgctccgggc tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg
     6721 tgggtttcct ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc
     6781 agtaccggaa cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg
     6841 gggccgcacg atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg
     6901 gcaaaggccg ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta
     6961 agactgtcac taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct
     7021 gccgattcga gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc
     7081 ctgaatgtga taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag
     7141 aaaggaggca ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat
     7201 tatgcgagaa ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg
     7261 atttcggcgt cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt
     7321 gtcttacagc agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt
     7381 tccagcgcct ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt
     7441 ttggtgaatt tcagaccgct ggtcgccgct tctgctactg gggtgaagaa tttctcttta
     7501 aagaaacgcg tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc
     7561 gggccgcagg tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga
     7621 gtgagacgac cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga
     7681 tagttacgcc actcatcggc gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc
     7741 cgcacatcgg cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg
     7801 acgccgcagc ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat
     7861 tcccccatcg cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac
     7921 tgggagccga caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct
     7981 ggcgtggagg aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt
     8041 gcatattgcc cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca
     8101 tccggcgttt cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc
     8161 atcgtcatcg acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg
     8221 tgatagaacg caggccgaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac
     8281 caggtgcagt cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa
     8341 tgacgatcgc tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttcacac
     8401 cacagcccag tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac
     8461 attgacctgc atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa
     8521 agcgatattt accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga
     8581 aatcggtgaa gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat
     8641 gacgcagttt ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct
     8701 ggcgaccaaa acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct
     8761 gttgcgcctg ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc
     8821 agccaacaat gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga
     8881 tgcagttcga ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct
     8941 gccagccgct gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat
     9001 gcgcaggcag catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca
     9061 gcaggttgta gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg
     9121 ctttgacgtt gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca
     9181 gaatgctcat cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt
     9241 tcatatcggc aggccaggtc cccagccacg ccatgatttt atggtactgg gcataaatat
     9301 cgttgtcgta ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc
     9361 catacaggca cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt
     9421 taggggtaaa ttctgctgcc tgacgttgca gctcgccgag atcatcgctc accagttgca
     9481 ggtcagccat cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgtgctttca
     9541 gcgcctcgcg caaggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt
     9601 taacgttggt cagggttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga
     9661 tgatgccgta ttcacacgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga
     9721 gtaaatcctg aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc
     9781 agccgtttcc tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac
     9841 cttttaaagt tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa
     9901 gggatttttc atgcaacttt aagggagatt gatttagcgc aattttggcg gcagggatct
     9961 accgccagag aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag
    10021 aataaaaccg tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac
    10081 gggcagggtg aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg
    10141 gcgaatcagc ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa
    10201 cgccgcgctc ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata
    10261 ccagccgaaa ccggaggcca ttgccagcgc ggtattgatg gggagatcaa gaataaaggc
    10321 gttaattaaa ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg
    10381 gcgattaagg acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag
    10441 gaaaagtagc aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag
    10501 tccactcaga ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag
    10561 cgccatcgca atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc
    10621 tcgctccagc cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc
    10681 agaataatgc agaatcgcca acaggttact ggcgaggtta tcgagaaacg ccagactgat
    10741 acccataaaa aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa
    10801 cgcagcttgt tggcgaagcg gaatgaggta acctacaatc aggggaacca gaatgattaa
    10861 cagcccagaa aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca
    10921 cactactgaa agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg
    10981 agcagtagag tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc
    11041 gatgttggtg gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga
    11101 tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag
    11161 ggataagtcg agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggggcaggg
    11221 ggcggcggcg attggccgcc ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag
    11281 ctcatgaaag gagcgcaaca agatgatgaa tcaacatata acaacatctt aaaaaaaggc
    11341 ctgacattac gccaggcctt ctgcgttaat taatcacgct tttccagcag ggtccggtaa
    11401 atcagaccac cgataatgcc gccgacaatt ggcaccaccc agaagaacca cagttgttct
    11461 aatgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg
    11521 ttagtcaccg gaatactaat taagtgaatc agggttaagg ccagaccaat agcgatcggc
    11581 gcaaaacctg ccggcgcgaa tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca
    11641 ctcaatacca gttcaactac cagcg
//
LOCUS       DAZOYS010000128        11517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOYS010000128 DAZOYS010000000
VERSION     DAZOYS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.128"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(205..1476)
                     /gene="efeB"
                     /locus_tag="ACHUN9_002970"
     CDS             complement(205..1476)
                     /gene="efeB"
                     /locus_tag="ACHUN9_002970"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0696310.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(1482..2609)
                     /gene="efeO"
                     /locus_tag="ACHUN9_002971"
     CDS             complement(1482..2609)
                     /gene="efeO"
                     /locus_tag="ACHUN9_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0696311.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2667..3497)
                     /gene="efeU"
                     /locus_tag="ACHUN9_002972"
     CDS             complement(2667..3497)
                     /gene="efeU"
                     /locus_tag="ACHUN9_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0696312.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(4163..5671)
                     /gene="putP"
                     /locus_tag="ACHUN9_002973"
     CDS             complement(4163..5671)
                     /gene="putP"
                     /locus_tag="ACHUN9_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0696313.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            6093..10067
                     /gene="putA"
                     /locus_tag="ACHUN9_002974"
     CDS             6093..10067
                     /gene="putA"
                     /locus_tag="ACHUN9_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0696314.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQGLLQEFS
                     LSSQEGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLL
                     FTGKLVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANA
                     RKLEEKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKL
                     SALHPRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLC
                     FEPELAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQ
                     MDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYY
                     PGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGA
                     NTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSA
                     GLDLANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVRE
                     ATPREVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTF
                     SNAIAEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALA
                     AGNSVLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVM
                     FTGSTEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAF
                     DSAGQRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANI
                     ERHIQTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHV
                     VRYNRNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVG
                     VQPFGGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQP
                     LNALREWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDE
                     QDALTQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIF
                     HGDSDQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASL
                     MTIG"
     gene            complement(10107..10745)
                     /gene="rutR"
                     /locus_tag="ACHUN9_002975"
     CDS             complement(10107..10745)
                     /gene="rutR"
                     /locus_tag="ACHUN9_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0696315.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            11033..>11517
                     /locus_tag="ACHUN9_002976"
     CDS             11033..>11517
                     /locus_tag="ACHUN9_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0696316.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         2767 a         3202 c         2943 g         2605 t
ORIGIN      
        1 actggaaaga tgacagagtg atgacagtga tgaaaaaagc tgtgtgcttt cagcaggatt
       61 tgcatcaggc atcagtgcac gatcgccgga tgcggcgtga acgccttatc cagcctacca
      121 aacccgcact gattgtaggc atgataagac gcgcaagcgt cgcatcaggc atctgcgcac
      181 aaatgcctta tccgcctaaa aacattaaac ccgcaataac gcgcttccga gataatcgtt
      241 cgcgtccttc actcccggca gcgaaaaaaa ataaccgccg ccgataggtt taacgtattc
      301 ctccagcgct tcgccattga gccttttttg tactgtcagg aagccttttt ccagatcgtg
      361 ttggtagcag acaaacagca accccatatc cagttgtccg gagttggtga cccccaacga
      421 ataactgtag ccgcgacgca gcatcaggct ggactcactc tccgccgtgc ggggattcgc
      481 cagccggata tggctgtcca gcgcaatcac cttcccttcc gggtcgctgg cgtaatcagg
      541 cacatcatgc tcatgctgca ttcccagcgg cgcaccggtt tgcttatcac ggccaaaaat
      601 cgtctgctgt tctttcagcg gcgttctgtc ccaaaattcc actcgaaact gaatcaagcg
      661 tactgcctga tagctgccac cgattgtcca cgcaggctcc tgctgatctg ccgttaccca
      721 caccactttt tgcatcaact tatcattctg gctatcggga ttggcagtgc cgtctttgaa
      781 acccagcaaa ttaatcggcg tctctttgcc tttactacgc gacacgtgat cggaaataaa
      841 cccttcccgc ttccagcgca cactgagcaa atccggcgtg tgtttgatga tatcgcgcag
      901 cgcatggata accgtgtcct gggtgttggc gcaaatctgt agcaacacat caccatgaca
      961 taacgccgca tccagcgagt cgttggggaa acgcgtcatc ttctgcagct tttttggcat
     1021 ctgtggcgca aggccaaagc gctcatcaaa caatgagtga cccaccgata acgtgatggt
     1081 gagattatcg ggcgcaatgt agccgccaag aatgccggaa tcgagtggtg gcaggcgcgg
     1141 atttggcgtt tctggtgctg ctccgccctg agtcagaaaa gcaaaacgct gagtcaacaa
     1201 gcgaaacaac cgctcaagat cggctttatc gctggcaagc acatcaaacg ccaccagcat
     1261 cattgcggcc tgttgtggcg tcaggatccc tgcctgatgc tcaccataaa acggctgttt
     1321 ctcattgcgt gcatccggtg aaagcgtgcc cggtgcactt tgcgtttttt gtgcatgagc
     1381 gaccggacaa cttcccgcca gcgccagcgc accaatccct ttcagtaaac gtcggcgtga
     1441 cggttcattc acgccgtttt catctttata ctgcataacg cttaatccag tcccagcaca
     1501 ccgcgaagtt gcgccagatc ttccgccagc gcagtaatcg gtcctttcag tgcattccgg
     1561 tcggcatcgg tcaatttgtc gtaggtttca aaaccgtctt tagtacggta tttcgccaga
     1621 atggtatcga cctttttaaa gttggcatcg acttttgcca gcagttccgg gttggctttt
     1681 tgcagttgtg gacgcagcaa atcgacaatt ttctgcgagc cttcaacgtt agcctggaaa
     1741 tcccacagat cggtgtggct gtagcgatct tcttcaccgc taattttgct ggctgccact
     1801 tcctcaatca gtccggctgc gccgccgacc acttttgaag gtgggaaagc cagttcactg
     1861 atgcgttttt gcaaatcgac cacatctgta taaagctgct cagcgtactg atccatccct
     1921 ttggtggtgt tgtcgccaaa caatgctttt tccagacggt ggaaaccagt gaattttggg
     1981 tcggcggctt tttgctcgta atcatcttca cgggcgtcaa tgctgccatc cagatcggag
     2041 aacagttcag caatcggttc aatgcgctca tagtgctggc gcgtcggcgc atacagtgct
     2101 ttcgcttttt cgatatcgcc tgctttaatc gcgtcggtaa aggctttggt gtcggtcacc
     2161 agctgcgtgg tttccgccat gacatacgct ttatatgcag taattgcacc accaagactt
     2221 aacagcgcat cactttgcgc cgcatccgcc gttgcctcac ctttgacgat caacttccct
     2281 ttcgggttag tcagcagacc gcaggtcata tcgtattcgc caggctgtaa attcgccgtc
     2341 attttctggc taaaaccagg ggcgatattt tcccgctctt ccaccaccat cacgcctttg
     2401 aggatctccc actccagcgc cttctggcta tggttctgaa taatgaactg tgttttcccg
     2461 gcgttaaccg taatggtcat cggttcgcac tgcttatccg tcacggtcac tttgacctgc
     2521 ggcacatcag cggcgttagc cataaaagca gaagaaaaca gcgcagccac gctcaactgc
     2581 aatgcgttac ggcggaagtt aatggtcatg acgagactat ccctttaaag tatgttgtaa
     2641 ctaagcaaga gtttgcgtcg tatttgttac gcggagcgag acgctgtcgc ccctgcgcgt
     2701 ggtggcagag caaatgccac cagcgccggg atgagataaa taaaccagac ggcgacttcg
     2761 ctgacgctcg gtgcttcctg atagccaaaa atcccttcca tcagcgtgcc aaacagcgag
     2821 tgagttgaga gcactgcgct catatcgaag gcgatttcct gaaagtggtt ccacaatccg
     2881 gcttcatgaa atgcgcgaat ggcaccagca gccagccctg cggcgacgaa gagaataaac
     2941 aggctggtcc atttaaaaaa tgcaccaaga ttgaggcgaa taccgcccca gtagagcagg
     3001 aagcctagca ccacggcagt agcaagaccg agcattgcac ccagcggcgg ccagatcccg
     3061 acatcttgtt gaaatgccgc cagcaggaaa aagaccgact ccagcccttc ccttgcaacg
     3121 gcaaaaaaga ccatcatcac cagcgcccag ccatgatgat ttccacgctg caatgcgcta
     3181 tcgactgcct gctccagttg tactttgacg ttgcgcgaca ctttgcgcat ccagaaaacc
     3241 atccaggtaa ggatcaccac ggcgatcacc gccacgatac cttcaaacag ttcctgttct
     3301 ttttgcggaa attcgccggt ggtttcgtta atgaagatac ccaagcccag gcacaacgca
     3361 gcggcaagca acacgccaat ccacatcacg ccaatccatc ggcctcgctg ggtacgctta
     3421 agatagctgg caatcaaact gacaatcagc gcggcttcaa gtccttcgcg caacataatg
     3481 agaaacggaa caaacatgcc acccaccctt aaacgttttt ctgagtcaat ccatgcaaag
     3541 aaaagtaaat cgcagtgata gcgattatca ttacggcaaa gaaaaataca agcggaatgt
     3601 ttataaaatg atgttggaat gtaaacaaag agaggtaata ccaggggata taaacgatta
     3661 ttccaggtac aaagatggga ttgtttacaa cgagccatgt gttcttgcca gatgccgcgt
     3721 aaacgcctta tccagccgac aaaactgtcc agactcaatg catcacagct tccatgtaaa
     3781 actgataagc gggtgtatca agcaatttcg tccatccgcg attatcaaat ctaacttttt
     3841 gcccggaaca taataacagc gcgggtgttg gtggcgtcaa aatcgctgaa acgttccccg
     3901 aaggccgggg cagcccacat ggatgtgggc tgagggcgcg tcttacagga cgttacctcg
     3961 cgcccgaccc ggtagccgaa ggggataagc tgaaggtacc gcgcagcggc gattttgttc
     4021 gccagagccc gggggtgcag ggggcggcgg cgactggccg ccccctgcgc gctccctgca
     4081 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
     4141 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
     4201 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
     4261 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
     4321 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
     4381 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
     4441 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
     4501 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
     4561 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
     4621 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
     4681 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
     4741 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
     4801 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
     4861 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
     4921 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
     4981 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
     5041 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     5101 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     5161 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     5221 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     5281 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     5341 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     5401 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     5461 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     5521 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     5581 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     5641 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     5701 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     5761 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     5821 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     5881 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     5941 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     6001 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     6061 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     6121 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     6181 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     6241 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     6301 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     6361 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     6421 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     6481 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccagg
     6541 ggttattgca ggggttattg caggagtttt cgctgtcatc gcaggaaggc gtggcgctga
     6601 tgtgtctggc agaagcgttg ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc
     6661 gcgacaaaat cagcaacggt aactggcagt cacacattgg tcgtagcccg tcactgtttg
     6721 ttaatgccgc tacctggggg ctgctgttta ctggcaaact ggtttccacc cataacgaag
     6781 ccagcctctc ccgctcgctg aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca
     6841 aaggtgtgga tatggcgatg cgcctgatgg gcgagcagtt cgtcacgggt gaaaccatcg
     6901 cggaagcgtt agccaatgcc cgcaagctgg aagagaaagg tttccgttac tcttacgata
     6961 tgctgggcga agctgcactg actgccgcag acgcacaggc gtatatggtt tcctatcagc
     7021 aggcgattca cgccattggt aaagcgtcta acggtcgtgg catctatgaa ggtccgggca
     7081 tttcaatcaa actgtcggcg ctgcatccgc gctacagccg cgcccagtat gaccgggtaa
     7141 tggaagagct ttacccgcgt ctgaaatcac tcaccctgct ggcgcgtcag tatgatattg
     7201 gtattaacat cgatgccgaa gaggccgatc gtctggagat ctccctcgat ctgctggaaa
     7261 atctctgttt cgagccggaa ctggcaggct ggaacggcat cggttttgtt attcaggctt
     7321 atcaaaaacg ctgcccgttg gtgatcgatt acctggttga tctcgccacc cgcagccgtc
     7381 gccgtctgat gattcgcctg gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc
     7441 agatggacgg ccttgaaggc tatccggttt atacccgcaa ggtgtatacc gacgtttctt
     7501 atctcgcctg tgcgaaaaag ctgctggcgg tgccgaatct aatctacccg cagttcgcga
     7561 cgcacaacgc ccatacgctg gcagcgattt atcaactggc aggtcagaac tactatccgg
     7621 gtcagtacga gttccagtgc ctgcatggta tgggcgagcc actgtacgag caggtcaccg
     7681 ggaaagttgc cgacggtaaa cttaaccgac cgtgtcgtat ttatgccccg gttggcacac
     7741 atgaaacgct gctggcgtat ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt
     7801 ttgttaaccg tattgccgac acctctttgc cactggatga actggtcgcc gatccggtca
     7861 ctgctgtaga aaaactggcg caacaggaag ggcaaactgg attaccgcat ccgaaaattc
     7921 ccctgccgcg cgatctttac ggtcacgggc gcgacaactc ggcagggctg gatctcgcta
     7981 acgaacaccg tctggcctcg ctctcctctg ccctgctcaa tagtgcactg caaaaatggc
     8041 aggccttgcc aatgctggaa caaccggtag cggcaggtga gatgtcgccc gttattaacc
     8101 ctgcggaacc gaaagatatt gtgggctttg tgcgtgaagc cacgccacgt gaagtagaac
     8161 aggcgctgga aagtgcggtt aataacgcgc caatctggtt tgccacgcct ccggctgaac
     8221 gcgcggcgat tttgcatcgc gctgccgtgc taatggaaag ccagatgcag caactgattg
     8281 gtattctggt gcgtgaagcc ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag
     8341 cggtcgattt tctccactac tacgccggac aggtgcggga tgatttcgct aacgaaaccc
     8401 accgtccatt agggcctgtg gtgtgtatca gtccgtggaa cttcccgctg gctattttca
     8461 ccgggcagat cgctgccgca ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac
     8521 aaacgccgct gattgccgcg caagggatcg ccattttgct ggaagcgggt gtaccccctg
     8581 gcgtggtgca attgctgcca ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg
     8641 atcgcgtgcg cggggtgatg tttaccggtt caaccgaagt cgctacgtta ctgcagcgca
     8701 atatcgccag ccgcctggac gctcagggtc gccctattcc gctcatcgct gaaaccggcg
     8761 gcatgaacgc gatgattgtc gattcttcag cactgaccga acaagtcgtc gtggatgtgc
     8821 tggcctcggc gttcgacagt gcgggtcagc gttgttcggc actgcgcgtg ctgtgcctgc
     8881 aagatgagat tgccgaccac acgttgaaaa tgctgcgcgg tgcaatggcc gaatgccgga
     8941 tgggtaatcc gggtcgcctg accaccgata tcggtccggt gattgatagc gaagcgaaag
     9001 ccaatattga gcgccatatt cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg
     9061 tgcgggaaaa cagcgaagat gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc
     9121 tgatcgaact ggatgacttt gccgaattgc acaaagaggt ctttggtccg gtgctgcatg
     9181 tggtgcgtta caaccgtaac cagctaccag agctgatcga gcagattaac gcttccggct
     9241 atggtctgac gcttggtgtc catacgcgta ttgatgaaac catcgcccag gtcactggct
     9301 cggcccatgt tggtaacctg tatgttaacc gtaatatggt gggcgcagtg gttggtgtgc
     9361 agccgttcgg cggcgaaggg ttgtccggta ccgggccgaa agcaggcggt ccgctctatc
     9421 tctaccgtct gctggcgaat cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg
     9481 atgcagagta tccggtcgat gcacagttga aagccgcatt gactcagccg ctaaatgcac
     9541 tgcgggaatg ggcagcaaat cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc
     9601 tggcgcaggc aggaacacaa cgattgctgc cggggccgac gggtgaacgc aacacctgga
     9661 cgctgctgcc gcgtgagcgc gtgttgtgta ttgccgatga tgagcaggat gcgctgactc
     9721 agctcgccgc cgtgctggcg gtgggcagcc aggtactgtg gccggatgac gcgctgcatc
     9781 gtcagctagt aaaggcattg ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg
     9841 aaaatataac cgctcaaccc tttgatgcgg tgatcttcca cggtgattcg gatcagcttc
     9901 gcgcattgtg tgaagccgtt gctgcgcggg atggcgcaat tgtttcggtg cagggttttg
     9961 cccgtggcga aagcaatatc cttctggaac ggctgtatat cgaacgttcg ctgagtgtga
    10021 ataccgctgc cgctggcggt aacgccagtt taatgacgat cggttaaaca ggtagccgga
    10081 ggatgttaca acctcctccg gcatctttaa cgtggtcgaa tcccctcaat aataatccgc
    10141 tgcacgtttt caaccgtttg attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc
    10201 gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc
    10261 aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga cccaaccggc aatcagcgca
    10321 gatttctcat caattaatgc cttcaaatcg cccgtcagtt catccattaa cagcggcgcg
    10381 cctgccagca tctccataca gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc
    10441 agcttcagac ggatgtactc ttttatcgcc gccagcggag cgaaatcttc acgaaacgct
    10501 tttaacggtg ccagccagat atcgagaatc tgccgcagca cggcaatata cagcgcctct
    10561 tttgacggaa agtaatacag cagattggtt tttgaaacac ccgccaactc tgcgatctgc
    10621 tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc
    10681 gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc
    10741 gtcatgcgct ctcccctctt gtagattggc aacaggatag caaaaccccc gcccggcctc
    10801 gaccttctgc actctcatcg cgcacagatg catgttttat gtgcaactgt tttgaccgtt
    10861 tagtccactt tttatcagat attttagata acgtaatatt aacatattaa taacattaac
    10921 tttttaaaaa ctggcatccg ctttgcaaac aagccaatac gcggctgaaa aggatatgca
    10981 ggatgcagcg ccccgtctga ctttcacttt acgagacgaa gagaggttga tgatgaaaat
    11041 tggcgtattc gtacctattg gcaacaacgg ctggctcatt tcgacccacg cgccgcagta
    11101 catgccgacc tttgaactga ataaagccat tgtgcagaaa gcggagcatt accatttcga
    11161 tttcgccctg tcgatgatca aactgcgtgg ctttggcggc aaaactgagt tctgggatca
    11221 caaccttgag tcgttcacct tgatggcggg tctggcagcc gtgacctcgc gcattcagat
    11281 ttacgccact gctgccacct taacgttacc tccggcaatc gtcgcccgta tggccgcaac
    11341 catcgactcc atctctggcg ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc
    11401 cgtgtatgag cagatgggta tctggcctgg cgatgactat ttctcccgtc gttacgacta
    11461 tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc acggggaaaa gcgattt
//
LOCUS       DAZOYS010000129        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOYS010000129 DAZOYS010000000
VERSION     DAZOYS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.129"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUN9_002977"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUN9_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0696317.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUN9_002978"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUN9_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0696318.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUN9_002979"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUN9_002979"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0696319.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUN9_002980"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUN9_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0696320.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUN9_002981"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUN9_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0696321.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUN9_002982"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUN9_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0696322.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUN9_002983"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUN9_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0696323.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUN9_002984"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUN9_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0696324.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUN9_002985"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUN9_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0696325.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUN9_002986"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUN9_002986"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0696326.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUN9_002987"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUN9_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0696327.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUN9_002988"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUN9_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0696328.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUN9_002989"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUN9_002989"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0696329.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZOYS010000130        11426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOYS010000130 DAZOYS010000000
VERSION     DAZOYS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.130"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHUN9_002990"
     CDS             460..1386
                     /locus_tag="ACHUN9_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0696330.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHUN9_002991"
     CDS             complement(1336..2529)
                     /locus_tag="ACHUN9_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0696331.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUN9_002992"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUN9_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0696332.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUN9_002993"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUN9_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0696333.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUN9_002994"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUN9_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0696334.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUN9_002995"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUN9_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0696335.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUN9_002996"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUN9_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0696336.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHUN9_002997"
     CDS             complement(11165..11308)
                     /locus_tag="ACHUN9_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696337.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2886 a         2880 c         2935 g         2725 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag tcccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaaaacaa aaaacgatca attagtattg
    11401 attcacataa tctctcatca ttaatg
//
LOCUS       DAZOYS010000131        11295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOYS010000131 DAZOYS010000000
VERSION     DAZOYS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.131"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUN9_002998"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUN9_002998"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0696338.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHUN9_002999"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHUN9_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0696339.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUN9_003000"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUN9_003000"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0696340.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUN9_003001"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUN9_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0696341.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUN9_003002"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUN9_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0696342.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHUN9_003003"
     CDS             complement(6652..8160)
                     /locus_tag="ACHUN9_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0696343.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUN9_003004"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUN9_003004"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0696344.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUN9_003005"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUN9_003005"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0696345.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAZOYS010000132        11244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOYS010000132 DAZOYS010000000
VERSION     DAZOYS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.132"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHUN9_003006"
     CDS             <1..309
                     /locus_tag="ACHUN9_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0696346.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHUN9_003007"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHUN9_003007"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0696347.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUN9_003008"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUN9_003008"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0696348.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUN9_003009"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUN9_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0696349.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUN9_003010"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUN9_003010"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0696350.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTLLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUN9_003011"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUN9_003011"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0696351.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUN9_003012"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUN9_003012"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0696352.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUN9_003013"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUN9_003013"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0696353.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUN9_003014"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUN9_003014"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0696354.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUN9_003015"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUN9_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0696355.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUN9_003016"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUN9_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0696356.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11244
                     /locus_tag="ACHUN9_003017"
     CDS             10354..>11244
                     /locus_tag="ACHUN9_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR0696357.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGV"
BASE COUNT         2554 a         3090 c         2952 g         2648 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcagcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg gaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cgtg
//
LOCUS       DAZOYS010000133        11241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOYS010000133 DAZOYS010000000
VERSION     DAZOYS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.133"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            6..140
                     /locus_tag="ACHUN9_003018"
     CDS             6..140
                     /locus_tag="ACHUN9_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0696358.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            119..223
                     /gene="speFL"
                     /locus_tag="ACHUN9_003019"
     CDS             119..223
                     /gene="speFL"
                     /locus_tag="ACHUN9_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0696359.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            534..2732
                     /gene="speF"
                     /locus_tag="ACHUN9_003020"
     CDS             534..2732
                     /gene="speF"
                     /locus_tag="ACHUN9_003020"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0696360.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2729..4048
                     /gene="potE"
                     /locus_tag="ACHUN9_003021"
     CDS             2729..4048
                     /gene="potE"
                     /locus_tag="ACHUN9_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0696361.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4104..5744)
                     /gene="pgm"
                     /locus_tag="ACHUN9_003022"
     CDS             complement(4104..5744)
                     /gene="pgm"
                     /locus_tag="ACHUN9_003022"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0696362.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5770..6315)
                     /gene="seqA"
                     /locus_tag="ACHUN9_003023"
     CDS             complement(5770..6315)
                     /gene="seqA"
                     /locus_tag="ACHUN9_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0696363.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6500..7264
                     /gene="ybfF"
                     /locus_tag="ACHUN9_003024"
     CDS             6500..7264
                     /gene="ybfF"
                     /locus_tag="ACHUN9_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0696364.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7404..7697
                     /gene="ybfE"
                     /locus_tag="ACHUN9_003025"
     CDS             7404..7697
                     /gene="ybfE"
                     /locus_tag="ACHUN9_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0696365.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7837..8367
                     /gene="fldA"
                     /locus_tag="ACHUN9_003026"
     CDS             7837..8367
                     /gene="fldA"
                     /locus_tag="ACHUN9_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0696366.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8577..8663
                     /gene="uof"
                     /locus_tag="ACHUN9_003027"
     CDS             8577..8663
                     /gene="uof"
                     /locus_tag="ACHUN9_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0696367.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8656..9102
                     /gene="fur"
                     /locus_tag="ACHUN9_003028"
     CDS             8656..9102
                     /gene="fur"
                     /locus_tag="ACHUN9_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0696368.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9187..9513)
                     /gene="chiQ"
                     /locus_tag="ACHUN9_003029"
     CDS             complement(9187..9513)
                     /gene="chiQ"
                     /locus_tag="ACHUN9_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0696369.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9563..10969)
                     /gene="chiP"
                     /locus_tag="ACHUN9_003030"
                     /pseudo
     CDS             complement(9563..10969)
                     /gene="chiP"
                     /locus_tag="ACHUN9_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2737 a         2768 c         2808 g         2928 t
ORIGIN      
        1 aattaatgat ttattatctg gggtatgatc gttttttgtt gatcttcttc acagattata
       61 gccatttcat ggatagaata actctacctt caactgacac agcaagaggt aaaggtaaat
      121 ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc
      181 ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc
      241 accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg tattcctcgg
      301 tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc acttgcaact
      361 tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag
      421 caaactactg atttatttat cagcggtgga gctttgcttt ttttccggcg tgatcgattt
      481 ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaat aaaatgtcag
      541 aattaaaaat tgccgttagc cgttcttgcc cggattgttt ttccactcat cgtgcatgcg
      601 tgaatattga cgaaagtaat tatattgacg ttgccgccat tattttatca gtcaatgatg
      661 ttgaacgtgg aaaactcgat gaaatagacg ctactggcta tggcattcct gtttttattg
      721 caacggaaaa tgaagaacgt gtaccagcag aatatctgcc acgtatttct ggtgtcttcg
      781 agcattgcga atcacggaaa gagttttatg gacgtcagtt agaaaccgct gctagccatt
      841 atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtc aatcagggca
      901 atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt catccggcag
      961 ggaatcagtt cgtggagtac ttcggagaaa cgctgttccg ttccgactta tgcaacgcag
     1021 acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc atgcattgca cagcaacatg
     1081 cggcaaaagt gtttaatgcc gataaaacat acttcgttct aaatggcact tcatcttcta
     1141 acaaagtggt tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt gatcgcaata
     1201 accacaaatc taaccaccac ggagcgttgc tacaggctgg tgcaacaccg gtttatctgg
     1261 aaacggcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt tttgaagaaa
     1321 gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag gctcgtcctt
     1381 tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac gcccgccagg
     1441 tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca tgggttggct
     1501 atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat cttaatgaga
     1561 acgatccggg tattctggtt acgcaatccg tacataaaca gcaggctggt ttttctcaaa
     1621 cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat gtaccgcaca
     1681 aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat ccgctgtttg
     1741 ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat atgtggatgg
     1801 attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt cagcatattc
     1861 gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa acagcgcaaa
     1921 ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat tcttttgaag
     1981 gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca accccaggta
     2041 ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt cttgctaact
     2101 tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taactccatc ctcttcctgc
     2161 tgactccggc agaagatatg gccaaacttc agcaacttgt tgccctgctg gtacgcttcg
     2221 aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc tacaaacagc
     2281 atgaagagcg ctacgccggt tataccctgc gtcagttgtg ccaggaaatg catgatttgt
     2341 atgcccgaca caacgtgaaa caactgcaaa aagagatgtt ccgcaaagcg cacttcccac
     2401 acgtcagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa gtggagctgg
     2461 ttcgtctgcc ggatgccgaa ggtcgtatcg ctgccgaagg ggcgcttcct tatcctccgg
     2521 gtgtgctgtg tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt tacttcagcg
     2581 ctctggaaga agggatcaac ctgctgccag gttttgcacc ggagctgcag ggtgtctata
     2641 tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct cgtgatgcgc
     2701 aaagcaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca aaatgggcgt
     2761 cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca tcatgctgcc
     2821 gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga cagccgtagg
     2881 ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta aatcaggtgg
     2941 tatgggcggt tatgccgagt atgctttcgg taaatccggt aactttatgg cgaactatac
     3001 ctacggcgtc tcactgctga ttgctaacgt cgcgattgct atttcggcgg ttggttacgg
     3061 caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga ccatcggggt
     3121 gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc aaatcagtag
     3181 cattaccgtg tggggggtca ttattccggt ggtaggcctg tgcattatcg gctggttctg
     3241 gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt tcttcagtgc
     3301 agtaggttct tccatcgcta tgacgctgtg ggcttttctc ggtctggagt ctgcgtgtgc
     3361 gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac tcggcggtac
     3421 gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga ttgtgcctaa
     3481 tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga tgttcacacc
     3541 agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt cgctgcttgg
     3601 ctggcagttc accattgccc aggtgtttaa gtcttcatct gatgaaggct acttccctaa
     3661 aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga ccattgtgat
     3721 tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc agttcaacgt
     3781 gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa tggcggcatt
     3841 agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg caaactttgt
     3901 tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg aagaagccat
     3961 gctgtacggt tccatcgtga ccttccttgg ttggacactg tatggtctgg tatcaccacg
     4021 ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg atcttgcgac
     4081 cgcccttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac aatctcaacc
     4141 gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca gtagatctta
     4201 tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc
     4261 agaccgccga tagaagcacc attgcccggt gccgccgtca ggcgcgcggt gatcgggtcg
     4321 cctgccaggg tgctggcgct caccatttcc ggagacagct tagacagcgc cgctttttgt
     4381 gcggaagtcg cagctgcctg caaacggttg tagctcggcg caccaaagcg ttttgccagt
     4441 tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga
     4501 cacatgatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg caggaaggaa
     4561 gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt cgaacagacc atcaacaaac
     4621 catttgaaac ctaccgggac ttctaccagc ttgcggccca agtcattgac cacacggtcg
     4681 atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga
     4741 tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc
     4801 ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta
     4861 tcacgcaatg ccagcaggcc cgccatcgca cactcggagg agcagtccat acggatcgcg
     4921 ccgtctttat cgaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc
     4981 aggttgaggt tgtaatactc gccaattcgc ttccagtatt cgataccgga accacccagc
     5041 ggatcaacgc ccagcgtcag gcccgttttc tgaatcgccg ccatatcaac gatatcggcc
     5101 agaccttcca caaactgctg caccagatcc tgctctttca catgaccgga tgccatcgct
     5161 tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccatcagtgc gttggcacga
     5221 tcttccacca ctttagtgac gttggtatca gccgggccac catttggcgg attgtacttg
     5281 ataccaccat cttccggcgg gttatgggag ggtgtaatca cgataccgtc tgccagcggg
     5341 ccaccttttt tattatgaac caggatggca ttggaaacgg caggcgtcgg agtgaagcca
     5401 ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg
     5461 aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgccg
     5521 tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag
     5581 ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca
     5641 ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa
     5701 tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc
     5761 tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga
     5821 ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca
     5881 atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg
     5941 ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa
     6001 cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg
     6061 cttttgctct gcgtattcgt cagaaagcag aagttcacgc attgcgcgaa ccttgtcttt
     6121 aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt
     6181 caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga
     6241 tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac
     6301 ttcaatcgtt ttcatcttaa tccagtacag tgtcttagct gaatacaagt cgccaagagt
     6361 atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata
     6421 aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg
     6481 cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca
     6541 caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca accttggcgt
     6601 actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg
     6661 tctttcaccg agagatccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac
     6721 tctggatgca cagcagatcg acaaagcaac atttatcggt cactccatgg gcggtaaagc
     6781 ggtaatggca cttacggcac tggcccgcga tcgcatcgat aaactggtgg cgatcgatat
     6841 cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcggcta tcaacgcggt
     6901 cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcacc agcatcttaa
     6961 tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt ggcgctttaa
     7021 cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg
     7081 ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg agcagtaccg
     7141 tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag gcgcgggtca
     7201 ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga
     7261 ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg gcgttggcgt
     7321 gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc tggctgattg
     7381 tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc
     7441 tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgaac
     7501 agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc cttaagcgtg
     7561 tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg gagtcgcgca
     7621 atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta
     7681 gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg
     7741 ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac
     7801 ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc
     7861 ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa
     7921 gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga agcttatgac
     7981 attctgctgc tgggcatccc aacctggtat tatggcgaag cgcagtgtga ctgggatgac
     8041 ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct gtttggttgt
     8101 ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat ccgcgacatc
     8161 attgaaccgc gcggtgcaac gatcgttggt cactggccaa ctgcgggcta tcatttcgaa
     8221 gcatcaaaag gtctggctga tgacgaccac tttgtcggtc tggctatcga cgaagaccgt
     8281 cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc tgaagagttg
     8341 catctcgacg aaattctcaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta
     8401 ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct
     8461 gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt
     8521 tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga
     8581 tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa
     8641 caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta
     8701 acgcttcctc gtttaaaaat cctagaagtt cttcaggagc cggacaacca tcacgtcagt
     8761 gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta
     8821 tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc
     8881 ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat ctgcctcgac
     8941 tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc
     9001 gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa
     9061 ggcgattgcc gcgaagatga acatgcgcac gaaggcaaat aagccagcct gaatgagaaa
     9121 aagccaacct gcgggttggc ttttttatgc aagggaaata agaaaagcga gagttacagc
     9181 tctcacttat ttgttattac tgcgaatttc ctgccaaact ttatcacaat cggctttcac
     9241 cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca gtacgcggac
     9301 actttcctgg tcggcaaaat gctgatgttg tttctctttc ttcagcacgt ttaacacgct
     9361 ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg cgctgtatgc
     9421 ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta ccagccccga
     9481 tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg tgcggcaggc
     9541 acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca
     9601 cgtcacgctc atcctggaag atgttgccgt aaccaccgcc ccagcttggg atgtcggagt
     9661 ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa
     9721 tggtgtagac cgcatccagg ctgtatgcag actcttcaat agtccggttt ttgtcgtagt
     9781 acgcatccgg attgctctgc taggtcgcag gtttagcatc ccatgcgtaa acgtaggaag
     9841 cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca ccgaagaaga
     9901 ccgctttttc gccgttagcg ttgaagtcgg aacggttatc ccaccagata tccaggcggc
     9961 cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc tgaccgtcag
    10021 ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg
    10081 tcaacgcttg cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga tcgtcaactt
    10141 tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt
    10201 tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca aatgccgctt
    10261 ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga taatcaactt
    10321 tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc gctttgtatt
    10381 cgttggtcca catgtaggag aaactcaacg caccagcatc gccgtaatca aaattcgccc
    10441 cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct aacagcgttt
    10501 gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttggccg
    10561 cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca taggctttat
    10621 tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc gccatttcaa
    10681 tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg gactgaaaat
    10741 cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg ccgtcggtaa
    10801 catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag gttgaatcgt
    10861 cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt gctgtaacac
    10921 cggcgatagc cagcgccagc gtactatgtt tgccactaaa cgtacgcatg ggttaatcct
    10981 ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa ttaaaaaagt
    11041 agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac gcgaattatc
    11101 aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt tgatgatttt
    11161 tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc gtgaaattcc
    11221 agcaagatga aagcggttac a
//
LOCUS       DAZOYS010000134        11218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOYS010000134 DAZOYS010000000
VERSION     DAZOYS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.134"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUN9_003031"
     CDS             53..352
                     /locus_tag="ACHUN9_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0696370.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUN9_003032"
     CDS             complement(472..651)
                     /locus_tag="ACHUN9_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0696371.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUN9_003033"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUN9_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0696372.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUN9_003034"
     CDS             2090..2668
                     /locus_tag="ACHUN9_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0696373.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUN9_003035"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUN9_003035"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0696374.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUN9_003036"
     CDS             4347..5945
                     /locus_tag="ACHUN9_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0696375.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHUN9_003037"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHUN9_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0696376.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHUN9_003038"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHUN9_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0696377.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHUN9_003039"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHUN9_003039"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0696378.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKTLKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHUN9_003040"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHUN9_003040"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0696379.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAIFWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHUN9_003041"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHUN9_003041"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0696380.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHUN9_003042"
     CDS             10721..11179
                     /locus_tag="ACHUN9_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0696381.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2756 a         2822 c         2830 g         2810 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaaac actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt tctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tattttta
//
LOCUS       DAZOYS010000135        10989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOYS010000135 DAZOYS010000000
VERSION     DAZOYS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.135"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            56..817
                     /gene="adiY"
                     /locus_tag="ACHUN9_003043"
     CDS             56..817
                     /gene="adiY"
                     /locus_tag="ACHUN9_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0696382.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            954..2291
                     /gene="adiC"
                     /locus_tag="ACHUN9_003044"
     CDS             954..2291
                     /gene="adiC"
                     /locus_tag="ACHUN9_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0696383.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2395..4038
                     /gene="eptA"
                     /locus_tag="ACHUN9_003045"
     CDS             2395..4038
                     /gene="eptA"
                     /locus_tag="ACHUN9_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0696384.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4035..4703
                     /gene="basR"
                     /locus_tag="ACHUN9_003046"
     CDS             4035..4703
                     /gene="basR"
                     /locus_tag="ACHUN9_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0696385.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4704..5804
                     /gene="pmrB"
                     /locus_tag="ACHUN9_003047"
     CDS             4704..5804
                     /gene="pmrB"
                     /locus_tag="ACHUN9_003047"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0696386.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5780..5869)
                     /gene="pmrR"
                     /locus_tag="ACHUN9_003048"
     CDS             complement(5780..5869)
                     /gene="pmrR"
                     /locus_tag="ACHUN9_003048"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0696387.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5981..7483)
                     /gene="proP"
                     /locus_tag="ACHUN9_003049"
     CDS             complement(5981..7483)
                     /gene="proP"
                     /locus_tag="ACHUN9_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0696388.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7747..8613)
                     /locus_tag="ACHUN9_003050"
     CDS             complement(7747..8613)
                     /locus_tag="ACHUN9_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0696389.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8610..10838)
                     /gene="crfC"
                     /locus_tag="ACHUN9_003051"
     CDS             complement(8610..10838)
                     /gene="crfC"
                     /locus_tag="ACHUN9_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0696390.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2747 a         2867 c         2694 g         2681 t
ORIGIN      
        1 gtatcgcttt gctttagcga gaactggtct tttattcgca ctcaggagta catgtatgag
       61 gatttgcagc gaccaacctt gtattgtttt attgactgaa aaagatgtct ggataagggt
      121 gaatgggaaa gaacctatta gccttaaagc taaccatatg gcgttattaa attgtgaaaa
      181 taatattatc gacgtctcct ctcttaacaa cactttggtt gctcatatta gtcacgacat
      241 catcaaagat tacctccggt ttctgaataa agatctctcg caaataccag tatggcaacg
      301 tagcgctacg cccatactca ccctgccatg cctgacgcca gacgtctttc gcgttgccgc
      361 gcaacacagc atgatgcccg cagaaactga gtcagaaaag gaacgaacac gcgcattatt
      421 attcacggtg ctatcccgtt ttctcgacag taaaaaattc ctttcattaa tgatgtatat
      481 gttacgtaat tgtgtaagtg acagcgttta tcaaattatt gaaagcgata ttcacaaaga
      541 ctggaatctt agtatggtag ccagttgttt atgtcttagc ccaagtctgt taaagaaaaa
      601 gctgaaaagc gaaaacacca gttatagcca aataatcacc acctgccgca tgcgttatgc
      661 cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag gtatcacagt cctgcggcta
      721 caacagtacg tcgtacttta tttctgtctt taaagacttc tacggtatga cgccgctgca
      781 ttatgttagt cagcacagag aacgaactgt cgcctgattt ttaaccttaa cgaagagcta
      841 tattaataac ggcatcagcg ataacccggt cgataataat tcaactatcg aatgcaggcg
      901 tatgatatga cgtaatttat tgtcacgaag ctcgccttcg caggagttta attatgtctt
      961 cggatgctga tgctcacaaa gtgggcttaa tccccgtcac cctgatggtg tcggggaata
     1021 ttatggggtc aggtgttttt ctgttacctg caaacctggc ctccactggc gggattgcca
     1081 tttatggatg gttggtgacg attatcggtg cgctggggct ctcgatggta tacgccaaaa
     1141 tgtcgttcct cgatccaagt cctggtggtt cttacgctta cgcccgccgc tgctttggcc
     1201 cgtttctcgg ttatcaaacc aacgtcctct actggctggc ctgctggatc ggcaatatcg
     1261 ctatggtggt cattggcgta ggatatttaa gttacttctt cccgattctg aaagatccat
     1321 tggtattaac catcacctgc gtcgtggtgc tgtggatctt cgtcctgctg aacattgtcg
     1381 gtccgaaaat gatcacccgt gtgcaggccg ttgccaccgt gctggcgctg atccccatcg
     1441 tcgggattgc cgtatttggc tggttctggt tccgtggtga aacctatatg gcggcatgga
     1501 acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac ccttaacgtt acgctgtggt
     1561 cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt ggtgaaaaac ccgaaacgca
     1621 atgtccctat cgccaccatt ggtggggtat tgattgccgc cgtttgctat gtgctctcca
     1681 ccaccgcgat tatggggatg atccctaatg ccgcactgcg cgtttctgcc tcgccattcg
     1741 gtgatgccgc acggatggcg ttgggtgaca ccgctggggc cattgtttcc ttctgcgcag
     1801 ctgcgggttg cttaggttca ctgggcggct ggacgttgct ggcgggtcaa acggcgaaag
     1861 ccgctgccga tgacggactg ttcccaccga tttttgcccg tgtaaataaa gcgggtacgc
     1921 cagtggcggg gctgattatc gtcggtattt tgatgaccat cttccagctc agtagcattt
     1981 cgccaaacgc aaccaaagag ttcggtctgg tatcttccgt gtcggtcatc tttacactgg
     2041 tgccatatct ttacacctgt gcggcgttac tgctgctcgg ccacggtcac tttggtaaag
     2101 cacgcccggc atatctggct gttactacca ttgccttcct ctactgcatc tgggccgtgg
     2161 tggggtccgg ggcgaaagag gttatgtggt catttgtcac cctgatggtc atcaccgcca
     2221 tgtatgcgct gaattacaac cggctacata aaaacccgta tcccttagat gcaccaataa
     2281 gcaaagatta attcccctta atccagcaaa cataaaagcc aaccttaaga acttaaggtt
     2341 ggcttaattt tgctttgcga gcatatgcgc actttgttcg atggaaacac cgtgatgttg
     2401 aagcgcctac taaaaagccc ctctttgaat ttactcgcct ggctattgtt ggccgctttt
     2461 tatatctcta tctgcctgaa tattgccttt tttaaacagg tgttgcaggc gctgccgctg
     2521 gattcgctgc ataacgtact ggttttcttg tcgatgccgg tcgtcgcttt cagcgtgatt
     2581 aatattgtcc tgacactaag ctctttctta tggcttaatc gaccactggc ctgcctgttt
     2641 attctggttg gcgcggctgc acaatatttc ataatgactt acggcatcgt catcgaccgc
     2701 tcgatgattg ccaatattat tgataccact ccagcagaaa gttatgcgct gatgacaccg
     2761 caaatgttat taacgctggg attcagcggc gtgcttgctg cgctgattgc ctgctggcta
     2821 aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt tccgtggagc caatattctg
     2881 gtttctgtac tgctgatttt gctggtcgcc gcactgtttt ataaagacta cgcctcgttg
     2941 ttccgcaata acaaagagct ggtgaaatcc ttaagcccct ctaacagcat tgttgccagc
     3001 tggtcatggt actcccatca gcgactggca aatctaccgc tggtgcgaat tggtgaagac
     3061 gcccaccgca acccgttaat gcagaacgaa aaacgtaaaa atttgaccat cctgattgtc
     3121 ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct acccgcgtga aactaacccg
     3181 cggctggcga aagataacgt ggtctatttc cctaataccg catcttgcgg cacggcaacg
     3241 gcagtttcag taccgtgcat gttctcggat atgccgcgtg agcattacaa agaagagctg
     3301 gcacagcacc aggaaggcgt gctggatatc attcagcaag cgggcatcaa cgtgctgtgg
     3361 aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg tgcctcacca gaacgtcacc
     3421 gcgctgaatc tgcctggtca gtgcatcaac ggcgaatgct atgacgaagt gctgttccac
     3481 gggctggaag agtacatcaa taacctgcag ggtgatggcg tgattgtctt acacaccatc
     3541 ggcagccacg gtccgaccta ttacaaccgc tatccgccgc agttcaggaa atttacccca
     3601 acctgcgaca ccaatgagat ccagacctgt accaaagagc aactggtgaa cacttacgac
     3661 aacacgctgg tttacgtcga ctatattgtt gataaagcga ttaatctgct gaaagaacat
     3721 caggataaat ttaccaccag cctggtttat ctttctgacc acggtgaatc gttaggtgaa
     3781 aatggcatct atctgcacgg tctgccttat gccatcgccc cggatagcca aaaacaggtg
     3841 ccgatgctgc tgtggctgtc ggaggattat caaaaacggt atcaggttga ccagaactgc
     3901 ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca atttattctc cacgctattg
     3961 ggattaactg gcgttgagac gaagtattac caggctgcgg atgatgttct gcaaacttgc
     4021 aggagagtga gtgaatgaaa attctgattg ttgaagacga tacgctgtta ttgcagggac
     4081 tgattctggc ggcgcaaacc gaaggctaca cgtgcgatgg cgtaacaacc gcgcggatgg
     4141 cggaacaaag ccttgaggcc ggtcattaca gcctggtggt actggattta gggttacccg
     4201 acgaagatgg actgcatttt ctcgcccgta tccggcagaa aaaatacacc ctgccggtac
     4261 tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc cgggctggat gtcggtgccg
     4321 atgactatct ggtgaagcct tttgcgctgg aagagttaca cgcccgtatc cgcgccctgc
     4381 tacgacgcca taataatcag ggcgaaagtg agctgattgt tggcaatctg acgctgaata
     4441 tgggtcgccg tcaggtatgg atgggcggtg aagagttgat tctgacgccc aaagaatatg
     4501 ctctgctgtc acggttaatg ctcaaagcag gcagtccggt acatcgggaa attctctaca
     4561 acgacatcta taactgggac aatgaaccct cgaccaacac cctggaagtg catatccaca
     4621 atctgcgcga caaagtgggc aaagcccgta tccgcaccgt gcgcggcttt ggctatatgc
     4681 tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt tctgcgccga ccaatatcgc
     4741 tgcgccaacg gctgatattg accatcgggg ccattttgtt ggtgtttgag ctgatcagcg
     4801 tcttctggct atggcatgaa agtaccgagc agattcagct gtttgagcag gcgctacgcg
     4861 acaatcgcaa caacgatcgc cacatcatgc gtgagatccg cgaggcggtc gccagcctga
     4921 ttgtccccgg cgtctttatg gtcagcctga cgctatttat ctgctatcag gcggtacgcc
     4981 gcatcacccg cccgctggcg gagctgcaaa aagagctgga agcgcgcacc gccgacaact
     5041 taacgcccat tgccattcac agcgccaccc tcgaaatcga agcggtggtt tcggcgttga
     5101 acgatctggt cagtcgcctg acgagcacgc tggataacga aaggttattt accgctgacg
     5161 tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt gcatctggaa ctgctggcga
     5221 aaacgcatca cattgatgta gcaccgttag tggcacggct tgatcagatg atggagagcg
     5281 tctcccagct gctgcaactg gcgcgtgccg gacagtcatt ttcttccggt aattatcaac
     5341 atgtaaaact gctggaagat gtcattctcc cctcgtatga cgaactcagt accatgctcg
     5401 accagcgaca gcaaaccctg ctattgccag agagcgccgc cgacatcacc gttcagggtg
     5461 atgcaaccct gctgcggatg ttattgcgaa acctggtaga aaacgcccat cgttacagcc
     5521 cacaaggcag caacattatg attaagctgc aagaagatgg cggagcggtc atggccgttg
     5581 aagatgaagg accgggtatt gatgagagta aatgcgggga gttgagtaaa gcgtttgtac
     5641 gtatggacag ccgttatggc gggattggtc tggggttaag tattgtcagc cgcattacac
     5701 agttgcatca cgggcagttt ttcctgcaaa accggcaaga gacttccggc acgcgggcct
     5761 gggtacggct gaagaaagat cagtacgtag caaaccagat ataaagaaag ctgctgacca
     5821 ccagcacact gaacacggta gttaaacttt cataaacacg gtttttcatt gcactctcct
     5881 ccgttaacct ggaggagagt atgcgcgtca gagattaagt gaaccttaag agttcaattg
     5941 gcctgataag acagcgtcac atcaggccat ccgtttcagc ttattcatca attcgcggat
     6001 gttgctgcac caggcgggta cgtttcgcct gcaaatcggc aatctcgtgg tcaatatcat
     6061 cgattttctg ctcgatatta tcgtaatgct cgacgagaat ttccttcgct tcctgtatat
     6121 ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc cgtctctttc atggttacgc
     6181 cggtgattaa accaatcacc gccactacca tcaggtaata ggcaggcatc atcagattct
     6241 gcgagctttc gaccagccag gccgccagtg ttggcgtcag accggcaacc agcaccgaaa
     6301 tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt cgggaacatc gctggcaagg
     6361 tagaagccat aacgcccgta aagcagttaa ggatcaccgc cagcatcagt aacccggcaa
     6421 aaatcaggcc gatgacgtta ctgttaatca gaataaacgc cgggatcgcc aacacaaaca
     6481 gggcaacact accaagtagc acaaacggac gacggccaaa acggtcactc agcaagccca
     6541 tcaccggctg gacaaacagc ataccgatca taatggcgat aataatcagc accccgtggt
     6601 cttcggagta atgcaggtta tgcgacaaat aactcggcat ataggtcagc aacatgtagt
     6661 aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa caggctgcgc cagtatttag
     6721 tggcaatctc tttaaacgag actttcgggc catcctgcaa accttcgcgg tcgccctgtt
     6781 ccagtttatc aacatgctgc tggaacgccg gagtttcttc cagcgcatgg cgcaggtaaa
     6841 gcccgataat ccctaacggc agagcaataa agaacggaat acgccagccc cagtcgagga
     6901 agttcgcttc gccgacaatg gtcgaaatta acaccaccac gcccgcaccc agcacaaacc
     6961 cggcaataga accgaagtcc agccagctgc ccataaagcc acgtttacgg tcaggggagt
     7021 attccgcaac aaatatcgac gccccggtat attcaccgcc gaccgagaaa ccttgtgcca
     7081 tcttacagat caacagcaga atcggtgccc aaataccaat cgtgtcgtag gacggtatta
     7141 agccaataca gaacgtactg atcgacataa tcacaatagt gatagcgagg atcttctggc
     7201 gaccatattt atcgcccaac ataccaaaga agagcccgcc aagcggtcga atcagaaagg
     7261 gaacggagaa agtggcaagt gcagcaacca tctgcacgct ggggtcagcc cccgggaaaa
     7321 aaactttacc taatgcgtaa gcaacaaaac cataaacacc aaaatcgaac cattccattg
     7381 cattacccag tgatgctgcg gtaatggctt tacgcagttt accgtcatca ataatggtga
     7441 catcacgaag ggtaatcggt tttacttttt tccttttcag catagctttc ctcgcagagt
     7501 tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg ccgcaaaagg ggagcgggaa
     7561 ttgcaggatg caatctctgt aacaagcgta gcgggtttgc ttacaccctc cggttaagga
     7621 atgtacaatc aaaccagttg acgcctgaat tgggcagtta atgacctttt taccctacca
     7681 gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt taggactcat tgatgtaact
     7741 gattatttac gccaaattta cagagaagat ttcctcgacc attgccccca ccaaacgctg
     7801 cgctgaacag agtcgtggca tccccagcgc gactgtttgc cagcgttggg tcactgacca
     7861 gcaaacgggc tgtttatctg ccgtggacca ctttcccagc caacttaaaa aatcagcatg
     7921 ctcccctccc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg gtgccgcatt
     7981 gatactctct gccagatgtt gtgtcgcttt ctcgcgcaaa tcacccagta acaacaccac
     8041 cgcttgcggg cttcccagac ggcaggcatc gccaaatgca ccccagcgca actcctccag
     8101 aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt
     8161 aaaaatttgc tcgctctgat cacctgcctg caccagtagg cctcgttgcg ttaacgcctt
     8221 ttccttccag cgacaacggg ctgaaaacca ctcatacaag gcggcaattt gctgggggag
     8281 ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgctgcgc
     8341 gatctgcgtc agcgccagat gaccgggatc cagcatcgac gccagttttt ccaccattgt
     8401 cagtcgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct gaagctgcac
     8461 acgcgaaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg cttgtggaag
     8521 gcgggcatca tcgccctgcg ccagatccac taaaaagcgg ggataaaccg actccagcac
     8581 gcgaccgggg ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga
     8641 atatcatcgc gtaacagctg ggcgtcggtg taaatccagc cgtgagtcat cacagtctgc
     8701 tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag agccaggctt
     8761 tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgca aaaagtattc
     8821 atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat ttgttggcaa
     8881 taaagatcga cttctgcgct taaagctcgt tgcaacacac tgtaatcaac cgtctctgtc
     8941 ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga aaaagtctca
     9001 cgcacaacgc ctaaacgcgt gctctgctcg tccgttgcat cctggcgcga aatgacttca
     9061 ctgaacagct ggcgagtgtt gacgtgggga actactccgt gaaaaacagg aaaatgaaac
     9121 ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat gggccgaata
     9181 acatcggtta acacccggca aagggtggat tcaagctcgg caaaacgcag cgtaaagtcg
     9241 cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcaggtacg aatctcgctt
     9301 aacggctccg aatcatcctg aaacaaggcg gctaactgcg cattcagcgc atcttgctgt
     9361 tgacgcagaa agtggttggc ggaggtcagg gccagctcga tttcatgttt aatctcgccg
     9421 ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt
     9481 tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc
     9541 gcctgctgcg cgtaattcaa cagtttatgc gcagcagatc gcagagcata caacgaggcg
     9601 ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac
     9661 aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc
     9721 ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cgctggtaac
     9781 ttaccgttgt tggctaactc atggcgcgct cgatttgcca agtagcccca catcgacgac
     9841 accggaaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga aatcagtgcc
     9901 cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca
     9961 tacagtggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc atcggagatc
    10021 gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg
    10081 ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac
    10141 gtcaactgac cgggataact ctccagcccc gccagatgga caaactccac ttcaatcacg
    10201 ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc
    10261 agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc acccagataa
    10321 tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat atctttatct
    10381 atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt
    10441 tgaattaaac aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc cttttgcccg
    10501 ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt aggcagaacc
    10561 tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatagt accgacaatc
    10621 gccagcacca tttccagtcg ggaaatttta ctcaactcat tattcagcat cgcgtgacgt
    10681 tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcgtcgtc tccgggtaca
    10741 gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca
    10801 gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc cttaaagcaa
    10861 attttgttac ttttatttag ccagattgtt tttgagttct gtttttggct tttataatta
    10921 ctgcaagaaa taattttata tttagtgtgt tgttttttat cagaataaat aacgtcttct
    10981 gatgcgttt
//
LOCUS       DAZOYS010000136        10853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOYS010000136 DAZOYS010000000
VERSION     DAZOYS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.136"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            80..421
                     /locus_tag="ACHUN9_003052"
     CDS             80..421
                     /locus_tag="ACHUN9_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0696391.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(412..1338)
                     /locus_tag="ACHUN9_003053"
     CDS             complement(412..1338)
                     /locus_tag="ACHUN9_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0696392.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1428..2426
                     /locus_tag="ACHUN9_003054"
     CDS             1428..2426
                     /locus_tag="ACHUN9_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0696393.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2423..2641)
                     /locus_tag="ACHUN9_003055"
     CDS             complement(2423..2641)
                     /locus_tag="ACHUN9_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0696394.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHUN9_003056"
     CDS             complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHUN9_003056"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0696395.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHUN9_003057"
     CDS             complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHUN9_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0696396.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHUN9_003058"
     CDS             5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHUN9_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0696397.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6891..7862
                     /gene="cysK"
                     /locus_tag="ACHUN9_003059"
     CDS             6891..7862
                     /gene="cysK"
                     /locus_tag="ACHUN9_003059"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0696398.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHUN9_003060"
     CDS             8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHUN9_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0696399.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHUN9_003061"
     CDS             8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHUN9_003061"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0696400.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10316..10825
                     /gene="crr"
                     /locus_tag="ACHUN9_003062"
     CDS             10316..10825
                     /gene="crr"
                     /locus_tag="ACHUN9_003062"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0696401.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2641 a         2797 c         2692 g         2723 t
ORIGIN      
        1 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
       61 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
      121 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
      181 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
      241 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
      301 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
      361 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
      421 atacaacgga gccccttccc aaccagccat tagcgcattg ccctggtctc gtaccacgtc
      481 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
      541 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
      601 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
      661 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
      721 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
      781 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
      841 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
      901 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
      961 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
     1021 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
     1081 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
     1141 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
     1201 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
     1261 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
     1321 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
     1381 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
     1441 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
     1501 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
     1561 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
     1621 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
     1681 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
     1741 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
     1801 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
     1861 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
     1921 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
     1981 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
     2041 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
     2101 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
     2161 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
     2221 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
     2281 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
     2341 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
     2401 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
     2461 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
     2521 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
     2581 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
     2641 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
     2701 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
     2761 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
     2821 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
     2881 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
     2941 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
     3001 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
     3061 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
     3121 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
     3181 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
     3241 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
     3301 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
     3361 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
     3421 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
     3481 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
     3541 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
     3601 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
     3661 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
     3721 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
     3781 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
     3841 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
     3901 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
     3961 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
     4021 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
     4081 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
     4141 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
     4201 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
     4261 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
     4321 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
     4381 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
     4441 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
     4501 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
     4561 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
     4621 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
     4681 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
     4741 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
     4801 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
     4861 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
     4921 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
     4981 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
     5041 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
     5101 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
     5161 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
     5221 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
     5281 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
     5341 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
     5401 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
     5461 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
     5521 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
     5581 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
     5641 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
     5701 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
     5761 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
     5821 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
     5881 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
     5941 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
     6001 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
     6061 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
     6121 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
     6181 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
     6241 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
     6301 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
     6361 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
     6421 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
     6481 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
     6541 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
     6601 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
     6661 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
     6721 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
     6781 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
     6841 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
     6901 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
     6961 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
     7021 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
     7081 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
     7141 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
     7201 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
     7261 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
     7321 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
     7381 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
     7441 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
     7501 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
     7561 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
     7621 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
     7681 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
     7741 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
     7801 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
     7861 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
     7921 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
     7981 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
     8041 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
     8101 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
     8161 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
     8221 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
     8281 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
     8341 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
     8401 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
     8461 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
     8521 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
     8581 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
     8641 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
     8701 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
     8761 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
     8821 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
     8881 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
     8941 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
     9001 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
     9061 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
     9121 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
     9181 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
     9241 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
     9301 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
     9361 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
     9421 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
     9481 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
     9541 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
     9601 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
     9661 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
     9721 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
     9781 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
     9841 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
     9901 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
     9961 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    10021 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    10081 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    10141 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    10201 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    10261 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    10321 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    10381 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    10441 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    10501 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    10561 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    10621 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    10681 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    10741 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    10801 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DAZOYS010000137        10834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOYS010000137 DAZOYS010000000
VERSION     DAZOYS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.137"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHUN9_003063"
     CDS             complement(<1..279)
                     /locus_tag="ACHUN9_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0696402.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHUN9_003064"
     CDS             complement(446..1186)
                     /locus_tag="ACHUN9_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0696403.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUN9_003065"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUN9_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0696404.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUN9_003066"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUN9_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0696405.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUN9_003067"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUN9_003067"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0696406.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUN9_003068"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUN9_003068"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0696407.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUN9_003069"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUN9_003069"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0696408.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHUN9_003070"
     CDS             7002..7433
                     /locus_tag="ACHUN9_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0696409.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHUN9_003071"
     CDS             7456..8034
                     /locus_tag="ACHUN9_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0696410.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHUN9_003072"
     CDS             8035..8742
                     /locus_tag="ACHUN9_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0696411.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHUN9_003073"
     CDS             8730..9407
                     /locus_tag="ACHUN9_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0696412.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHUN9_003074"
     CDS             9401..10078
                     /locus_tag="ACHUN9_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0696413.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHUN9_003075"
     CDS             complement(10050..10763)
                     /locus_tag="ACHUN9_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0696414.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10834)
                     /locus_tag="ACHUN9_003076"
     CDS             complement(10760..>10834)
                     /locus_tag="ACHUN9_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0696415.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2581 a         2879 c         2698 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tatttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgc
//
LOCUS       DAZOYS010000138        10786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOYS010000138 DAZOYS010000000
VERSION     DAZOYS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.138"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUN9_003077"
     CDS             92..1132
                     /locus_tag="ACHUN9_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0696416.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUN9_003078"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUN9_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0696417.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUN9_003079"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUN9_003079"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0696418.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUN9_003080"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUN9_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0696419.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUN9_003081"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUN9_003081"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0696420.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUN9_003082"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUN9_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0696421.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUN9_003083"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUN9_003083"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR0696422.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUN9_003084"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUN9_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0696423.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2752 a         3001 c         2628 g         2405 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatg
//
LOCUS       DAZOYS010000139        10687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOYS010000139 DAZOYS010000000
VERSION     DAZOYS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.139"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUN9_003085"
     CDS             complement(<1..784)
                     /locus_tag="ACHUN9_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0696424.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUN9_003086"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUN9_003086"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0696425.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUN9_003087"
     CDS             1860..2747
                     /locus_tag="ACHUN9_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0696426.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUN9_003088"
     CDS             2780..3790
                     /locus_tag="ACHUN9_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0696427.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUN9_003089"
     CDS             3860..5293
                     /locus_tag="ACHUN9_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0696428.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUN9_003090"
     CDS             5392..6741
                     /locus_tag="ACHUN9_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0696429.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUN9_003091"
     CDS             6731..7618
                     /locus_tag="ACHUN9_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0696430.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHN
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUN9_003092"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUN9_003092"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0696431.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUN9_003093"
     CDS             8352..9224
                     /locus_tag="ACHUN9_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0696432.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUN9_003094"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUN9_003094"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0696433.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUN9_003095"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUN9_003095"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0696434.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2463 a         2531 c         2854 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcata atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZOYS010000140        10658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOYS010000140 DAZOYS010000000
VERSION     DAZOYS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.140"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHUN9_003096"
     CDS             <1..656
                     /locus_tag="ACHUN9_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0696435.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUN9_003097"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUN9_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0696436.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUN9_003098"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUN9_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0696437.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHUN9_003099"
     CDS             2258..2494
                     /locus_tag="ACHUN9_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0696438.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHUN9_003100"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHUN9_003100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
     gene            complement(2654..2740)
                     /locus_tag="ACHUN9_003101"
     tRNA            complement(2654..2740)
                     /locus_tag="ACHUN9_003101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2704..2706),aa:Leu,seq:cag)
     gene            complement(3008..4039)
                     /gene="rsmC"
                     /locus_tag="ACHUN9_003102"
     CDS             complement(3008..4039)
                     /gene="rsmC"
                     /locus_tag="ACHUN9_003102"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0696439.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4142..4555
                     /gene="holD"
                     /locus_tag="ACHUN9_003103"
     CDS             4142..4555
                     /gene="holD"
                     /locus_tag="ACHUN9_003103"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0696440.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4524..4970
                     /gene="rimI"
                     /locus_tag="ACHUN9_003104"
     CDS             4524..4970
                     /gene="rimI"
                     /locus_tag="ACHUN9_003104"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0696441.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4985..5662
                     /gene="yjjG"
                     /locus_tag="ACHUN9_003105"
     CDS             4985..5662
                     /gene="yjjG"
                     /locus_tag="ACHUN9_003105"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0696442.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5753..7342
                     /gene="prfC"
                     /locus_tag="ACHUN9_003106"
     CDS             5753..7342
                     /gene="prfC"
                     /locus_tag="ACHUN9_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0696443.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7735..8340
                     /gene="osmY"
                     /locus_tag="ACHUN9_003107"
     CDS             7735..8340
                     /gene="osmY"
                     /locus_tag="ACHUN9_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0696444.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8467..8628
                     /locus_tag="ACHUN9_003108"
     CDS             8467..8628
                     /locus_tag="ACHUN9_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0696445.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8750..9823
                     /locus_tag="ACHUN9_003109"
     CDS             8750..9823
                     /locus_tag="ACHUN9_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0696446.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9820..10602
                     /locus_tag="ACHUN9_003110"
     CDS             9820..10602
                     /locus_tag="ACHUN9_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0696447.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2593 a         2665 c         2845 g         2555 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca cagtcatctt actttttttg
     2641 atatcgcctc gtttggtgcg aggggggaga cttgaacccc cacgtccgta agaacactaa
     2701 cacctgaagc tagcgcgtct accaattccg ccaccttcgc ataccatcaa ttcttaaaaa
     2761 gaattgctac cacggaggcg cattctagtg gttttcagct tttcgtcaat agttaattat
     2821 cgccggaggt gtaattgctg gaaaaatgtc catcaggaaa ctagcgtgca ggtttggtag
     2881 gcatgcgggg gcagatgcca gatgcgacgc tggcgcgtct tatctggcct acgaagggct
     2941 aacgtgcagg ttttgtaggt cggataaggc gttcacgccg catccgacac ggtattcggc
     3001 gagataatta acctttcttc gcctggcggg tcataatggc gcgatacacc ttgaaacgcc
     3061 cggtttgcgc aatcacttca tggaagccaa atgtctcatc cagcacgtcc gggtaaggca
     3121 ggaaggcgtt cgctacaatt cgcagctcgc cgccgctatt aagatgacgc accgcgccgc
     3181 gaatcagcgt ttgcgccgca tccaggctgg tttgcatccc atcgtggaac ggcgggttgg
     3241 agatgatcat atcaaaacga cctttcacct cggaaaagac gttgctggca aagacttcac
     3301 cttcaacaca gttggccgca agtgttgcgc ggctggcttc taccgctggc gcagagacat
     3361 cgcacaaggt gagacgaatt ttcggcgaat ggcgcgcaaa ggcaactgaa agcacccccg
     3421 cgccacagcc gacatccagc actttacctt tcgtgtgcgg agttaacgtc gagagcagca
     3481 actggctacc gacatccaga ccgtcgcggc taaacacgcc aggcagcgtt ttgaccgtca
     3541 ggccatcgac gctgtattcg ccccagaatt tatcggcatc aaataccggc tgtttttcca
     3601 gacgaccaaa atagaggcca cagcgacgag cgctgtcgac tttattcaac ggcgcataat
     3661 ctgccagcat ctgctcggcg ctgcgcacgc cgctgcggtt ctcgccaacg acaaaaatat
     3721 ctgtccccac tggcagcaga gaaagtaaat tcatcaactg gaactgggct tccggtttgt
     3781 tcttcggcca gtagtaaatc agcgtatcgc aatctgcgac gtcatccacc gtggcgacca
     3841 gactgaaacg ggcgttatcc cccatctggc ggcttaatac ctgccagtgg tggaattgct
     3901 gggtatgagc acggctggcc gcggtatcta aacgcgcggg caggtcatcc tgtaagtctc
     3961 cggcaaacag aatacggctt tgttcgaaat catcactgtg acgcagcaag acttcacttg
     4021 ccggggtaaa tgcagacatg gaatgctcct caattgatac tggcggcgat tatagccata
     4081 tgttggcgcg gtatcgacga atttgctata tttgcgcccc tgacaacagg agcgattcgc
     4141 tatgacatcc cgacgagact ggcagttaca gcaactgggc attacccagt ggtcgctgcg
     4201 tcgccctggc gcgttgcagg gggagattgc cattgcgatc ccggcacacg tccgtctggt
     4261 gatggtggca aacgatcttc ccgccctgac cgatccttta gtgagcgatg ttctgcgcgc
     4321 attaaccgtc agccccgatc aagtgctgca actgacgcca gaaaaaatcg cgatgctgcc
     4381 gcaaggcagt cgctgcaaca gctggcggtt gggtactgac gaaccgctat cactggaagg
     4441 cgctcaggtg gcatcaccgg cgctcaccga attacgggca aacccaacgg cacgcgccgc
     4501 gttatggcaa cagatttgca catatgaaca cgatttcttc cctcgaaacg actgatttac
     4561 cagcggctta ccacattgaa caacgcgccc acgcctttcc gtggagtgaa aaaacctttg
     4621 ccagcaacca gggcgagcgt tatctcaact ttcagttaac gcaaaacggc aaaatggcgg
     4681 cgtttgcgat tacgcaagtg gtgctggatg aagctacatt gttcaatatt gcggtcgatc
     4741 ctgactatca gcgtcaggga ttgggaaggg tgctgctgga acatctgatc gacgaactgg
     4801 aaaaacgcgg cgtggcgaca ctatggctgg aagtccgtgc ctcaaatgct gccgccattg
     4861 ccctgtacga aagtttaggc tttaacgagg cgacgattcg ccgcaattac taccccacca
     4921 cggacggtcg cgaagacgcc atcatcatgg cgttgccaat cagtatgtaa gacaaggtgg
     4981 aataatgaag tgggactgga ttttctttga tgccgatgaa acgctgttta cctttgactc
     5041 attcaccggc ctgcagcgga tgtttcttga ttacagcgtc acctttaccg ctgaagattt
     5101 tcaggactat caggccgtta acaagccact gtgggtggat tatcaaaacg gcgcgatcac
     5161 ttcattacag cttcagcacg ggcgttttga gagctgggcc gaacggctga aagttgaagc
     5221 aggcttgctt aacgatgcct ttattaatgc gatggcggaa atctgcacgc cgctgccggg
     5281 cgcggtttct ctgcttaacg ccattcgtgg caacgccaaa atcggcatca tcaccaacgg
     5341 ctttagcgcc ttgcagcaag tgcgtctgga acgcacgggc ctgcgtgatt acttcgattt
     5401 gctggtgatt tccgaagaag ttggcgttgc caaaccgaat aagaaaattt tcgattatgc
     5461 gctggaacag gcgggcaatc ctgaccgttc acgcgtgctg atggttggcg acactgccga
     5521 gtccgatatt ctcggtggca tcaacgccgg gcttgcgacc tgctggctga atgcgcacaa
     5581 tcgcgagcaa ccagaaggca tcgcgcccac ctggaccgtt tcttcgttgc acgaactgga
     5641 gcagctcctg tgtaaacact gattgcctcc cccccgttga tgggtaaaat agccgcaatt
     5701 tttcgttttc aacaagcgcg gcgcgatgcc gcttactcaa gaagaaagaa ttatgacgtt
     5761 gtctccttat ttgcaagagg tggcgaagcg ccgcactttt gccattattt ctcacccgga
     5821 tgccggtaag actaccatca ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc
     5881 cggtacagta aaaggccgtg gttccaacca gcacgctaag tcggactgga tggagatgga
     5941 aaagcagcgt gggatctcca ttactacgtc tgtgatgcag tttccgtatc acgattgcct
     6001 ggttaacctg ctcgacaccc cggggcacga agacttctcg gaagatacct atcgtaccct
     6061 gacggcggtg gactgctgcc tgatggttat cgacgctgca aaaggtgttg aagatcgtac
     6121 ccgtaagctg atggaagtta cccgtctgcg cgacacgccg atcctcacct ttatgaacaa
     6181 acttgaccgt gatatccgcg acccgatgga actgctcgat gaagttgaga acgagctgaa
     6241 aatcggctgc gcaccgatca cctggccgat tggctgcggc aagctgttta aaggcgttta
     6301 ccacctttat aaagacgaaa cctatctcta tcagagcggt aaaggccaca cgattcagga
     6361 agtccgtatt gttaaagggc tgaataaccc ggatctcgac gctgcggttg gtgaagatct
     6421 ggcacagcag ctacgtgacg aactggaact ggtgaaaggc gcgtctaacg agttcgacaa
     6481 agaattgttc cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc tgggtaactt
     6541 cggcgttgat catatgctgg atggcctggt ggagtgggct cctgcgccga tgccgcgtca
     6601 gactgatacc cgtaccgtag aggcgagcga agacaaattt accggcttcg tatttaaaat
     6661 tcaggccaac atggacccga aacaccgcga ccgcgtggcg tttatgcgcg tagtatccgg
     6721 taaatatgaa aaaggcatga agctgcgcca ggtgcgtact gcgaaagatg tggttatctc
     6781 cgacgcgctg acctttatgg cgggcgaccg ttcgcacgtt gaagaagcgt atccgggcga
     6841 tatcctcggc ctgcacaacc acggcactat tcagatcggc gacaccttta cccagggtga
     6901 gatgatgaag ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc gtatccgcct
     6961 gaaagatccg ctgaagcaaa aacagctgct caaagggctg gtacagcttt ccgaagaggg
     7021 cgcggtgcag gtgttccgtc cgatctccaa caacgatttg atcgttggtg ctgttggtgt
     7081 gctgcagttt gatgtggtgg tatcgcgcct gaagagcgaa tacagcgttg aagcagtata
     7141 tgaatcagtc aacgttgcca ctgcccgctg ggtagaatgt gcggacgcga agaaattcga
     7201 agagttcaag cgtaagaacg aaagccaact ggcgcttgat ggcggcgata acctcgctta
     7261 catcgctacc agcatggtca acctgcgcct ggcgcaggaa cgttatcccg acgttcagtt
     7321 ccaccagacc cgcgagcatt aattcttgcc tccagggcgc ggtagccgct gcgccctgtc
     7381 aatttccctt ccttattagc cgcttacgga atgttcttaa aacattcact tttgcttatg
     7441 ttttcgctga tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa agtaatgaca
     7501 tttctgacgg cgttaaatac cgttcaatgc gtagatatca gtatctaaag ccgtcgattg
     7561 tcattctacc gatattaata actgattcag aggctgtaat ggtcgttatt catcacccat
     7621 cgcttttgtg atggcgacca ttgacttctg tagagggtga agtctctccc tattcagcaa
     7681 tgcaacctcg tgttgccagg ctcaaattac gagcaaacat acaggaataa atcgatgact
     7741 atgacaagac tgaagatttc gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg
     7801 accggctctg cctacgcgga aaacaacgca cagactacca atgaaagcgc agggcaaaaa
     7861 gtcgatagct ctatgaataa agtcggtaat ttcatggatg acagcgccat caccgcgaaa
     7921 gtgaaggcgg cgctggtgga tcatgacaac atcaagagca ccgatatctc tgtcaaaacc
     7981 gatcaaaaag tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc cgaagaggca
     8041 gtgaaagtgg cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact gcacgttcgc
     8101 gacgctaaag aaggctctgt gaagggctac gcgggtgaca ccgccaccac cagtgaaatc
     8161 aaagccaaac tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc
     8221 gacggcgtgg ttcagctctc cggtaccgtc gattcccagg cacagagcga ccgtgctgaa
     8281 agtatcgcca aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa
     8341 ttcgtcgtaa ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc acactaaaaa
     8401 tatcgcattg agtgattttc acgctcatat caagcggtga cattaactat ggtaaaggag
     8461 acgcttatgt ttcgttgggg catcatattt ctggttatcg cgttaatcgc cgccgcactt
     8521 gggtttggtg gtctggccgg taccgctgca ggcgcagcta aaattgtctt tgtcgtcggg
     8581 attattctgt tcctggtgag tttgttcatg ggccgaaaac gaccctagat ttcagaccat
     8641 taagaaaatt ttatccaaag ccagtccagc ggactggctt ttgcggtttt agcgaataat
     8701 aaattgcgtt actttgtcat tctttcacaa caaaaacagg aaggcagagg tggggcagcg
     8761 aatacctgta acgcttggta atattgcgcc gttgtcgcta aggccgttcc agcctggacg
     8821 aatagctctg gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg gcgtgctgga
     8881 tgagtttatg cgcgcgcagt ttaatccttt cgatctttat ctcggcacat ctgccggggc
     8941 gcagaacctc tcggcgttta tctgcaatca gcccggttac gcgcgcaaag tcatcatgcg
     9001 ctataccaca aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag gaaatcttat
     9061 cgatctcgac tggctggtgg aggccaccgc aagccagatg ccgttgcaaa tggataccgc
     9121 cgcgcggttg tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc aggatgacta
     9181 cgcgcctaat tactttttac caaccaaaca aaactggctg gatgtgattc gcgcctccag
     9241 tgcgatacct ggcttttatc gtagcggagt gtcgctggaa ggcattaact acctggatgg
     9301 cgggatcagt gatgcgattc cggttaaaga ggcggcaagg cagggcgcta aaacgttggt
     9361 cgtcattcgc actgtgccgt cacaaatgta ctacacgccg cagtggttca aacgcatgga
     9421 acgctggctg ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc atcatgaaac
     9481 cagctatcgt gacattcagc aatttattga gaaaccaccg ggcaagctgc ggatattcga
     9541 aatttatccg ccgaagccat tacatagtat cgcgcttggc agtcggattc cggcgctgcg
     9601 tgaagactat aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg ttggcaagct
     9661 attaactgaa aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc cggaatcgat
     9721 cgttattccg cctgcgccag tcgccaacga tacgctggtt gccgaagtga gcgatgctcc
     9781 gcaggcgaac gacccgacat ttaacaatga ggatctggct tgatttgccg ttttatcgat
     9841 acccactgcc attttgattt cccgccgttt agtggcgatg aagaggccag cctgcaacgc
     9901 gcggcacaag cgggcgtagg caagatcatt gttccggcga cagaggcgga aaattttgcc
     9961 cgcgtgcagg cgttagcgga aaaatatcaa ccgctgtatg ccgcattggg gttgcatccg
    10021 gggatgttgg aaaaacatag cgatgtgtct cttgatcagc tacagcaggc gctggaaagg
    10081 cgtccggcga aggtggtggc ggtgggggag atcggtctgg atctcttcgg cgacgatccg
    10141 caatttgaga ggcagcagtg gttactcgac gaacagctga aactggcgaa acgctacgat
    10201 ctgccggtga tccttcattc ccggcgtata cacgacaaac tggcgatgca tcttaaacgt
    10261 cacgatttac cgtgcactgg cgtggttcac ggtttttccg gcagcctgca acaggccgag
    10321 cggtttgtac agctgggcta caaaatcggc gtaggcggta ctatcaccta tccacgcgcc
    10381 agtaaaaccc gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct ggaaaccgac
    10441 gcgccggata tgccgctcaa cggctttcag gggcagccta accgaccgga acaggctgtg
    10501 cgggtgtttg acgtgctttg cgagttgcgc ccggaaccag aagatgagat tgccgaagtg
    10561 ttgcttaata atacgtatgc gttgtttagc gttagtgggt agcctaccct tcgtaggcag
    10621 catccggcag tcggcgcata atgcctgatg cgacgctt
//
LOCUS       DAZOYS010000141        10531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOYS010000141 DAZOYS010000000
VERSION     DAZOYS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.141"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHUN9_003111"
     CDS             complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHUN9_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0696448.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /gene="malX"
                     /locus_tag="ACHUN9_003112"
     CDS             1275..2867
                     /gene="malX"
                     /locus_tag="ACHUN9_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0696449.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="ACHUN9_003113"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="ACHUN9_003113"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0696450.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4153..5154
                     /gene="add"
                     /locus_tag="ACHUN9_003114"
     CDS             4153..5154
                     /gene="add"
                     /locus_tag="ACHUN9_003114"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0696451.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAKR
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5190..6230)
                     /locus_tag="ACHUN9_003115"
                     /pseudo
     CDS             complement(5190..6230)
                     /locus_tag="ACHUN9_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6474..6599
                     /gene="blr"
                     /locus_tag="ACHUN9_003116"
     CDS             6474..6599
                     /gene="blr"
                     /locus_tag="ACHUN9_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0696452.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHUN9_003117"
     CDS             6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHUN9_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0696453.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7165..7605
                     /locus_tag="ACHUN9_003118"
     CDS             7165..7605
                     /locus_tag="ACHUN9_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0696454.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHUN9_003119"
     CDS             7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHUN9_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0696455.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHUN9_003120"
     CDS             8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHUN9_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0696456.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8834..>10531
                     /gene="rsxC"
                     /locus_tag="ACHUN9_003121"
     CDS             8834..>10531
                     /gene="rsxC"
                     /locus_tag="ACHUN9_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0696457.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     "
BASE COUNT         2405 a         2724 c         2693 g         2709 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc ggcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcaggccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac accctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcttcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agtagccacg atgtcatcac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgccagt catgttctgt atcgccatcc cgctgggtct ggcacgcgaa aacaaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg acgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgaca ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgattcc ataatatccg cctgccggat gcgcttgcat
     1801 tcttcggcgg tacgcgcttc gtaccaatca tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg cgatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cattgaccat cttccaggcg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggcaaaatgc
     2161 ctgcgtttct cggcggtctg ccaggtgcag cgttagcgat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa gggctgctga tttctggcct gatcgcctgt gtcgttggcg
     2281 gcacgaccga accgctggaa ttcctgttcc tgttcgtggc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcactg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gtcatcttcc
     2521 gtttcgctat cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa gtggccagtt
     2581 caatcgaaaa agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt ccggcaatcc
     2641 tcgaagcctt aggcggtgcc gacaatattg ttagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatcgag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgcgg ctattgccca ctggttttcc acccagcatt
     3121 acaccgcaat tgatacgcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacacg cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga cggctggttt tgcgatatgg gcaagctgga agccgttttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatga tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtc tggggcgagc agccgcatat tccctggagt aatgtggcgc
     3541 ggggagactg ggcgttgctg acgtcgggat cgaaaagttt caatattccc gcgctgaccg
     3601 gtgcttacgg gattatagaa aatagcagca gccgtgatgc ctatttatcg gcactgaaag
     3661 gacgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggtgc accgtggctg gatgccttac gcgtctatct gaaagataac ctgacgtata
     3781 tctcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatcccg caatccactt
     3841 atctggcctg gcttgatcta cgtccgttga atattgacga caacgcgttg caaaaagcgc
     3901 ttatcgagca agaaaaagtc gcgatcatgc cggggtatac ctatggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taataaacgc catccgcgct gttcgctaac cccaattgcg caacgtaaaa aatcgttgcg
     4081 caatcgtgga tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa
     4141 aagagtgcga ccatgattga taccaccctg ccattaactg atatccatcg ccaccttgat
     4201 ggcaacattc gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct
     4261 gcacaatccc tggaaacact gattccccac gttcaggtca ttgccaacga acccgatctg
     4321 gtgagctttc tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcctgc
     4381 cgccgcgtgg catttgaaaa cattgaagat gcagcccgta acggcctgca ctatgtcgag
     4441 ctgcgttttt caccaggcta catggcaatg gcacatcagc tgcctgtagc gggtgttgtc
     4501 gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt
     4561 atcggcatta tgagccgaac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt
     4621 ttagcccacc gtgaccagat taccgcactt gatttagctg gtgatgaact tggattcccg
     4681 ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc
     4741 catgcaggcg aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg
     4801 aagcgtattg gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc
     4861 gagcaacaaa ttggtattga atcctgtctg acctccaata ttcagaccag caccgtagca
     4921 gagctggctg cacatccgct gaaaacgttc cttgagcatg gcattcgtgc cagcattaac
     4981 actgacgatc ccggcgtaca gggagtggat atcattcacg aatataccgt tgccgcgcca
     5041 gctgctgggt tatcccgtga gcaaatccgc caggcacaga ttaatggtct ggaaatggct
     5101 ttcctcagcg cagaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa gtaacaaaaa
     5161 tggatggtgc aatttgcacc atccattttt cacgcaaggc acaacgtcgc gcgatgtttg
     5221 gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctagct tgccggaacc
     5281 ggattttcac catcgccatt taacgcatca cgaatcgccg catagtaagc cggataattc
     5341 ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg
     5401 ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt tttcagacgt
     5461 tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg cacgatatac
     5521 cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag
     5581 gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg tgccaaatct
     5641 accgtcatgc tgaccggtaa accaaatagc gtaatggcct gatcaagaag atgcggtgct
     5701 aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc gcgcacctgc
     5761 ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac gccttccgcg
     5821 agcaaacctt ttagcgtcaa gaaatcgcta tcccaacgac ggttatggaa tacagacagc
     5881 acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag tgtcacggta
     5941 aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc taacgggaaa
     6001 tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt aaacagatgc
     6061 ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt ttcatcactg
     6121 ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt
     6181 ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat gtcattgcct
     6241 ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac agtttaataa
     6301 cctgccagca ataagggatg ttgtttaact taagtcaaaa aaaatagcga attttccaac
     6361 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
     6421 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
     6481 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
     6541 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
     6601 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
     6661 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
     6721 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
     6781 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactcta cccctttggt
     6841 attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa tttcgcaaaa
     6901 tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc ctgacggacg
     6961 atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag ctggtttctg
     7021 gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat gtccaataaa
     7081 gaaagtagcg atcggttata aaagccttat aataacgccc ctaaaattag atttgcctgt
     7141 ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat tggaggctgg
     7201 ttgcttggcc cgttggcttg gcttttagtc gctctgttga gtacgacgct ggcgttgttg
     7261 ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg acaagcactg
     7321 acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg gtattacaca
     7381 ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca ctatattatc
     7441 tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc cgtggaagat
     7501 ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat cgtcccctat
     7561 ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc ttacagttaa
     7621 cctgttgtcg cctgctctgg attaacggat aataggcggc ttttttattt caggccgaaa
     7681 aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact ttgtactggt
     7741 caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa ccgcgatggg
     7801 catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct ggcttatcga
     7861 tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat ttattctggt
     7921 gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc cggtgcttta
     7981 ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc tcggcgtggc
     8041 gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg gtttttccgc
     8101 cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgcgaac gccttgctgt
     8161 ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg caggtcttat
     8221 gtctctggcc tttatgggct ttaatggttt ggtgaagttg taatgaatgc tatctggatt
     8281 gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg ttatgcctcc
     8341 cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat cttaccgcag
     8401 agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc catcagctgt
     8461 aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct aaaaattgcc
     8521 gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct aacgcctgcg
     8581 cggatggtgg cggttattga tgaaaataac tgtattggct gcactaaatg tattcaggct
     8641 tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat gagtgatctc
     8701 tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc gttgcaaccg
     8761 gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt gcgtatcatt
     8821 cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa tctgggattt
     8881 caacggcggc atccatccac cggagatgaa aacccagtcc aacggtacac ccctgcgcca
     8941 ggtaccactg gcgcagcgtt ttgttattcc actgaaacag catattggcg ctgaaggtga
     9001 gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc gtggtcgcgg
     9061 caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg cgccccactc
     9121 tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg atggtgaaga
     9181 ctgctggatc ccgcgcgacg gctgggccga ttatcgcact cgcagtcgcg aagagttaat
     9241 cgagcgcata catcagtttg gtgttgccgg gctgggcggt gcaggattcc cgacaggcgt
     9301 taaattgcag ggtggcggag ataagattga aacgttgatt atcaacgcgg ctgagtgcga
     9361 gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg tcgtagaggg
     9421 tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca ttgaagataa
     9481 caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg atatttctct
     9541 gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct acattctgac
     9601 cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa tgcaaaacgt
     9661 cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta ccgagcgtgt
     9721 tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac ggctggggac
     9781 gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc aaatggtgat
     9841 tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg tcgtaaagat
     9901 taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg aagaacaaag
     9961 ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg gatcttttgc cgcaacagtt
    10021 gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca ttgctgattg
    10081 cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccctgg tgcaatattt
    10141 ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg ccgcagaagc
    10201 caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg ctcgccttga
    10261 acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga ttgctgccgc
    10321 tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga ttaaagcggg
    10381 cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag cgcaagccag
    10441 agcgaaacag gcagaactgc agcaaactaa cgacgcagca accgttgctg atccacgtaa
    10501 aactgccgtt gaagcagcta tcgcccgcgc c
//
LOCUS       DAZOYS010000142        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOYS010000142 DAZOYS010000000
VERSION     DAZOYS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.142"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUN9_003122"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUN9_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0696458.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUN9_003123"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUN9_003123"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0696459.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUN9_003124"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUN9_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0696460.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUN9_003125"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUN9_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0696461.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUN9_003126"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUN9_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUN9_003127"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUN9_003127"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0696462.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUN9_003128"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUN9_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0696463.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUN9_003129"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUN9_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0696464.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUN9_003130"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUN9_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0696465.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2556 a         2522 c         2763 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZOYS010000143        10438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOYS010000143 DAZOYS010000000
VERSION     DAZOYS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.143"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            76..651
                     /gene="ssuE"
                     /locus_tag="ACHUN9_003131"
     CDS             76..651
                     /gene="ssuE"
                     /locus_tag="ACHUN9_003131"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0696466.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            644..1603
                     /gene="ssuA"
                     /locus_tag="ACHUN9_003132"
     CDS             644..1603
                     /gene="ssuA"
                     /locus_tag="ACHUN9_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0696467.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1600..2747
                     /gene="ssuD"
                     /locus_tag="ACHUN9_003133"
                     /pseudo
     CDS             1600..2747
                     /gene="ssuD"
                     /locus_tag="ACHUN9_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2759..3550
                     /gene="ssuC"
                     /locus_tag="ACHUN9_003134"
     CDS             2759..3550
                     /gene="ssuC"
                     /locus_tag="ACHUN9_003134"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0696468.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3547..4314
                     /gene="ssuB"
                     /locus_tag="ACHUN9_003135"
     CDS             3547..4314
                     /gene="ssuB"
                     /locus_tag="ACHUN9_003135"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0696469.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4521..7133)
                     /gene="pepN"
                     /locus_tag="ACHUN9_003136"
     CDS             complement(4521..7133)
                     /gene="pepN"
                     /locus_tag="ACHUN9_003136"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0696470.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7399..8601
                     /gene="pncB"
                     /locus_tag="ACHUN9_003137"
     CDS             7399..8601
                     /gene="pncB"
                     /locus_tag="ACHUN9_003137"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0696471.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8771..10171
                     /gene="asnS"
                     /locus_tag="ACHUN9_003138"
     CDS             8771..10171
                     /gene="asnS"
                     /locus_tag="ACHUN9_003138"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0696472.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2344 a         2692 c         2826 g         2576 t
ORIGIN      
        1 cttgtctctc cgacagactg accgaattgt tatcaatgtt aacaaaaaaa gaacaattgg
       61 ttataaggag agagtatgcg tgtcatcacc ctggcgggta gtcctcgctt tccttctcgc
      121 tccagctcct tgctggaata tgcgcgggaa aaactaaatg gcctggatgt agaggtttat
      181 cactggaatc tgcaaaactt cgccccggaa gatctgcttt acgctcgttt cgatagtccg
      241 gcactcaaga ccttcaccga acagctgcaa caggctgatg gactgatagt cgctacacct
      301 gtgtataaag ccgcctattc cggcgcgctg aaaaccctgc tcgacctgct gccagagcgc
      361 gctttgcaag gcaaagtggt gctaccgctg gcgacgggcg gtacggtggc tcatctgctg
      421 gcggtcgatt atgcccttaa gccagtttta agtgcgatga aagcccagga gctcctgcac
      481 ggcgtgtttg ccgatgactc acaagttatt gattaccatc acaaacccca gttcacgcca
      541 aatctgcaaa cccgtcttga taccgcgcta gaaactttct ggcaggcatt gcaccgccgc
      601 gatgttcagg ttcctgacct tctgtctctg cgaggtaatg tccatgcgta acatcattaa
      661 actggcgctg gcgggattgc ttagcgtctc tacttttgcg gttgctgcag aatcctcgcc
      721 tgaagcgtta cgtataggct atcagaaagg cagtattggt atggtattgg caaaaagcca
      781 ccagttactg gaaaaacgct atccgcaatc aaaaatctcc tgggtggagt tccccgcggg
      841 tccgcaaatg ttggaagcgt taaacgttgg cagtattgat ctcggcagta ccggggatat
      901 tccgccaatc tttgcccagg ctgccggggc tgatttgata tatgtgggcg tcgagccacc
      961 gaagcccaaa gccgaagtga ttctggtggc agaaaacagc ccgatcaaaa ccgtagccga
     1021 tcttaaaggt cacaaagttg cctttcagaa aggttccagt tcacacaacc ttttactgcg
     1081 tgcactgcgt caggccggac ttaagtttac tgacatccag cccacttatc tgacgccagc
     1141 tgatgcccgc gccgcgttcc agcaaggtaa cgttgacgcc tgggctatct gggatcccta
     1201 ctactccgct gcgttattac agggcggcgt gcgggtgctg aaagacggca ccgatctcaa
     1261 tcaaactgga tcgttttatc tggcagctcg cccctatgca gaaaaaaacg gcgcttttat
     1321 tcagggcgta ctggcaacct ttagtgaggc cgatgcgtta acccgcagcc agcgcgagca
     1381 aagcatcgct ttactggcaa aaacgatggg cttaccggca ccggtgattg cctcgtatct
     1441 ggatcatcgt cctcccacca ccatcaaacc ggtgagcgct gaagttgccg ccttacagca
     1501 gcaaacggca gatctgtttt atgaaaaccg tctggtgtcg aaaaaagttg atattcgcca
     1561 gcgcatctgg cagcccactc acctggaagg aaaacaatta tgagtctgaa tatgttctgg
     1621 tttttaccga cccatggtga cgggcattat cttggaacgg aagaaggttc acgcccggtt
     1681 gatcacggct atctgcaaca aattgcgcaa gcggcggatc gtctgggcta taccggtgtg
     1741 ctgatcccaa cggggcgatc gtgtgaagat gcgtggctgg tggcggcgtc gatgatcccg
     1801 gtgacgcagc ggctgaagtt tcttgtcgcc ctgcgtccca gcgtaacctc acctaccgtt
     1861 gccgctcgcc aggccgccac gcttgaccgt ctctcaaatg gtcgtgcgtt gtttaatctg
     1921 gtcacaggca gcgatccaca agagttggca ggcgacggcg tgttccttga tcatagcgag
     1981 cgctacgaag cctcggcgga atttacccag gtctggcggg gcgcttattg ctgggggaaa
     2041 gcgtcgattt caacggtaaa catattcatg tgcggggagc aaaactgctc ttcccgccga
     2101 ttcaacagcc gtatcctccg ctttactttg gcggatcgtc agatgtcgcc caggagctgg
     2161 cggcagaaca agttgatctc tacctcacct ggggcgaacc gccagagctg gtaaaagaga
     2221 agatcgaaca agtgcgggcg aaagctgccg cgcatggacg caaaattcgt ttcggtattc
     2281 gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg gcaggccgcc gagcggttaa
     2341 tctcgcatct tgatgatgaa actatcgcca aagcacaggc cgcattcgcc cggacggatt
     2401 ccgtaggaca acagcgaatg gcggcgctac ataacggcaa gcgcgacaat ctggagatca
     2461 gccccaattt atgggcgggc gttggcttag tgcgcggcgg tgccggaacg gcgctggtgg
     2521 gcgatggtcc tacggtcgcc gcgcgaatca acgaatacgc cgcgcttggc atcgacagct
     2581 ttgtgctttc gggctacccg catctggaag aagcgtatcg ggttggcgag ttgctgttcc
     2641 cacatctgga tgtcgccatt ccggaaattc cccagccgca gccgctgaat ccgcaaggcg
     2701 aagcggtggc gaatgatttt atcccccgta aagtcgcgca aagctaagga acacgatgat
     2761 ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc tggtttttac cggtgggcat
     2821 cgtggcggtg tggcaactgg cctcctcggt tggctggctg tcgacgcgta ttttgccttc
     2881 accggaagga gtggtgacgg cgttctggac gctctccgcc agcggcgaat tgtggcagca
     2941 tctggcgatc agttcctggc gggcgctgat tggcttttca attggcggat cgctgggctt
     3001 gattctggga ctgattagcg ggctgtcacg ctggggagaa cggctgctgg atacctcaat
     3061 tcagatgttg cgcaacgtac cgcatctggc gctgatcccg ctggtgattt tgtggtttgg
     3121 cattgatgaa tccgcaaaaa tctttctggt ggcgctcggc acgctattcc ccatttatat
     3181 caacacctgg catggcatcc gtaatatcga tcgcggtctg gtggaaatgg cgcgtagcta
     3241 tggattatcc ggcataccgc tgtttatcca tgtgatcctg cctggtgccc tgccctcaat
     3301 tatggtcggc gtgcgttttg cgttgggcct gatgtggctg acgctgattg ttgccgaaac
     3361 catttctgcc aattcaggca ttggttatct ggcgatgaat gcgcgggagt ttttgcaaac
     3421 ggacgtggtg gtagtcgcta ttattcttta cgccctgctc ggcaaacttg ctgacgtcag
     3481 cgcgcagctg ttagagcgcc tctggttgcg ctggaacccg gcttatcatt tgaaggaggc
     3541 cacggtatga atactgctcg tctgaaccag ggcacaccat tgttgctcaa tgcagtaagc
     3601 aaacattacg cggaaaatat cgtcctgaac caactggatt tacatattcc ggcaggtcag
     3661 tttgtggcgg tggtgggccg cagcggtggt ggcaaaagta ccctgctgcg cctgctggca
     3721 ggtctggaaa cgccaaccgc aggcgatgtg ttagcgggca ccacaccgct ggctgagatt
     3781 caggaagaca cgcgaatgat gtttcaggat gcgcgtctgc tgccgtggaa atcggtgatt
     3841 gataacgttg ggttaggcct taaaggccag tggcgcgatg ccgcgcgtcg ggcgttggct
     3901 gcagtaggac tggagaatcg cgccggggaa tggcctgccg cactttctgg cgggcagaaa
     3961 cagcgtgtgg cgctggcaag agcgttgatt catcgaccgg gtttattgtt gcttgatgaa
     4021 ccgctcgggg cactggacgc cttaacgcga ctcgagatgc aggatttgat tgtgtcactt
     4081 tggcagcagc acggctttac cgtactgttg gtgacgcatg atgtgagcga agcggtagca
     4141 atggctgacc gggtgttgtt aattgaagag ggaaaaattg gtctggattt gacagtggat
     4201 atcccccgtc cgcgtcgatt agggtcggtg cgattagcgg agctggaagc ggaagtattg
     4261 caacgggtaa tgcggcgtgg gcattctgaa caactgattc ggcgtcatgg ttaatatgcc
     4321 ggatgtggcg cacaaaacca gcattttcaa cggacttgta ggcctgataa gacacgtcaa
     4381 gcatcgcatc aggcagttgg gcacggctgc cggatgcggc gtgaacgcct tatccagcct
     4441 acaaaaccag caatttcaac ggagttgtag gcctgataag acgcgtcaag cgtcgcatca
     4501 ggcattttgg ctcaaaagta tcaagccagt gctttggtga tcttctcgta cagatcgcca
     4561 gagagatttt ccagcccttt caactgttcc agcgccgcgc gcattttctc ctgacgtttg
     4621 gcatcataac gtttcaggcg aatcagcggt tcaatcagac gtgaagctac ctgcgggtta
     4681 cggctgttga ggtcggtaag catttccacc aggaactggt aaccgctgcc atcttcggca
     4741 tggaacgctg ccgggttgct gcccgcaaac gcaccaatca acgaacgaat acggttcggg
     4801 ttgctcatgg taaatgagcg atgctgcaac agaccacgca ccgtctccag cacattcgcc
     4861 gccgggctgg tggcttgcag gataaaccat ttatccatca ccagaccgtc ctgatgccac
     4921 ttatcgtcgt actcctgcat cagcgcgtca cggcaaggca gctgtgcagc aaccgccgca
     4981 gaaagcgctg ccagcgcatc ggtcatattg tttgcttcat ggtattgctt gctcaccagc
     5041 acgtcagcca gatgcgtttc accaaaagca aggaagcgca ggcaggcatt tcgcagagtg
     5101 cgttttgcaa tatcttcatg ctcaacacgg tactcgctct ggtaattcgc gttgtaaata
     5161 gccagcagct catccgccag ttcagtcgcc agagtacgag tgagtgcttc gcgtacttcg
     5221 gcaatagcaa tcgggtcgat gatatcgaac agttcagcca tttcattgac agaaggcagc
     5281 gtcaggattt ctgccgccag cgccggatca atcttctcat cgagcagcac cgcgcggaaa
     5341 gcgtcagcca catgtaccgg cagagacagc ggctgccctt gctgatgacg ggcgacgttc
     5401 agcttgatgt aggttgccag caaactttgc gccgcatccc aacgggagaa atcattacgc
     5461 gcatgacgca tcaggaaggt cagttgctga tcactccact tatattccag tttcactggc
     5521 gcagagaatt cgcacagcag cgcaggcacc ggctggaagt agacattatc aaaaacaaag
     5581 gtctgttccg cctgggtgac gttcagcacg gaattcaccg gatgaccgcc tttctgcaac
     5641 gggatcactt tgccttcgtt gtcatacaat tcgatggcaa acggaatatg cagcggctgt
     5701 ttttctgcct gatccggcgt ggctggcgtg cgctggctga tggtcagggt gtactgctca
     5761 gtttccggat tgtagtcgtc tttgacggtc acaatcggcg taccggactg gctgtaccaa
     5821 cggcggaaat gggagagatc aacattcgac gcatcttcca tcgcctgcac aaagtcgtca
     5881 caggtcgctg cgctaccatc atgacgctcg aaatagagtt gcatcccttt ctggaagttt
     5941 tcttcgccca gcagggtgtg gatcatacgg atcacttccg cgcccttctc gtaaacggtc
     6001 agggtgtaga agttgttcat ctcaatgacc atgtccgggc ggattgggtg cgccatcggg
     6061 ctggcgtctt ccgcaaactg caatccgcgc atggtgcgca cattattgat gcggttaact
     6121 gcgcgggaac caaggtcaga gctgaattcc tgatcgcgga agacggttaa accttctttc
     6181 aggctgagct ggaaccagtc gcggcaggtc actcggttac cggtccagtt atggaaatat
     6241 tcatggccga taacgcgttc aatatcgagg taatctttgt cggtggcggt gtcggtgcgg
     6301 gccagcacat atttggagtt aaagatattc agacccttat tctccattgc gcccatattg
     6361 aagaaatcca ccgcgacgat catatagatg tcgaggtcat actccaggcc gaagcgttct
     6421 tcatcccatt tcattgagtt tttcagcgag gtcatcgccc acggcgcgcg atcaaggttg
     6481 ccgcgatcga cgtacagctc cagcgccact tcgcgaccag aacgcgtggt aaaggtgtcg
     6541 cgcagtacat caaagtcgcc tgccaccagc gcaaacaggt agcacggttt cgggaacggg
     6601 tcctgccact gtacccaatg gcgtccgttt tccagttcac cttgcgcaac gcggttaccg
     6661 ttggaaagca ggaagggata tttggtttta tcggcaataa ttttggtggt aaaacgcgcc
     6721 agcacgtccg ggcggtcgag ataatacgta atatggcgga aaccttcggc ttcacactgg
     6781 gtgcaaagcg catcgcctga ctgataaagc ccttccagcg cggtattcgc cgccgggctt
     6841 atttcattaa cgatcttaag cgtaaaatgc tccggcaaat tactgatgac cagtgcgccc
     6901 tcttcttctt tccaggcggt ccacggctca tcattaatat gaacagaaac cagtttgagg
     6961 tcttcgccgt tgagacgaag tggagcatct gatgcaccat gacggacagc ctggctgacc
     7021 gcggtaacga ccgtcttttg cgcgtcgagg tcaaaggtca agtcaatatc agtaatctgg
     7081 taatccggcg cacgataatc gtgacggtat ttggcttgtg gctgttgagt cataaataac
     7141 ctttagcatc ttttatagag tctggtgttc agtctattcc tgttgcgtaa atcgcgctat
     7201 gcagaatctt catcttttca ggtacaaacg cctttattgc tacattttta taacatacac
     7261 cgcgtaatgc catcgaccag aaaggtggca tatggtgtga tcggggttca ataaattgcg
     7321 aaacaaggta tactccagca gttcctgaag atgtttattg tactaaacgc tcctgtacga
     7381 ggacgctact gcgcacctat gacacaattc gcttctcctg ttctgcactc gttgctggat
     7441 acagatgctt ataagttgca tatgcagcaa gccgtgtttc atcactatta cgatgtgcat
     7501 gtcgcggcgg agtttcgttg ccgaggtgac gatctgctgg gtatttatgc cgatgctatt
     7561 cgtgaacaga ttcaggcgat gcagcacctg cgcctgcagg atgatgaata tcagtggctc
     7621 tctgccctgc ctttctttaa ggccgactat cttaactggt tacgcgagtt ccgctttaac
     7681 ccggaacaag tcaccgtgtc caacgataat ggcaagctgg atattcgctt aagcggtccg
     7741 tggcgtgaag tcatcctctg ggaagttcct ttgctggcgg ttatcagtga aatggtacat
     7801 cgctatcgct caccgcaggc cgacgttgcg caagccctcg acacgctgga aagcaaatta
     7861 gtcgacttct cggcgttaac cgccggtctt gatatgtcgc gcttccatct gatggatttt
     7921 ggcacccgtc gccgtttttc tcgcgaagta caagaaacca tcgttaagcg tctgcaacag
     7981 gaatcctggt tcgtgggcac cagcaactac gatctggcgc gtcggctttc cctcacgccg
     8041 atgggaacac aggcacacga atggttccag gcacatcagc aaatcagccc ggatctagcc
     8101 aacagccagc gagctgcact tgctgcctgg ctggaagagt atcccgacca acttggcatt
     8161 gcattaaccg actgcatcac tatggatgct ttcctgcgtg atttcggtgt cgagttcgct
     8221 agtcggtatc agggcctgcg tcatgactct ggcgacccgg ttgaatgggg tgaaaaagcc
     8281 attgcacatt atgaaaagct gggaattgat ccacagagta aaacgctggt tttctctgac
     8341 aatctggatt tacgcaaagc ggttgagcta taccgccact tctcttcccg cgtgcaatta
     8401 agttttggta ttgggactcg cctgacctgc gatatccccc aggtaaaacc cctgaatatt
     8461 gtgattaagt tggtagagtg taacggtaaa ccggtggcga aactttctga cagccctggc
     8521 aaaactatct gccatgataa agcgtttgtt cgggcgctgc gcaaagcgtt cgaccttccg
     8581 catattaaaa aagccagtta atatcatcag ggagctaatc ggctcccttt ttttaccttt
     8641 aattccgaaa tctttcgctg catttgcgaa ttctgcttgt ctgattgcag atgccaggta
     8701 acataggtat cccccccatt tacgggtgga caagtgttta ttttttccga ctattaacag
     8761 agagaatatt atgagcgttg tgcctgtagc cgacgtactc cagggccgcg tagccgttga
     8821 cagcgaagtc accgtacgcg gatgggtacg tacccgccga gattcaaaag ctggcatctc
     8881 cttcctcgcc gtttatgacg gttcctgctt tgatcctgta caggctgtca tcaataattc
     8941 tctgcccaat tacaatgaag acgtcctgcg tctgaccacc ggctgctcgg tcattgtgac
     9001 gggtaaagtc gtggcatcgc cgggccaggg gcaacaattt gaaattcagg ccagcaaggt
     9061 tgaagttgct ggttgggttg aagatccaga cacttacccg atggcggcaa aacgccacag
     9121 cattgagtat ctgcgtgaag tcgctcacct gcgtccgcgc acaaacctga ttggtgccgt
     9181 cgcgcgcgtt cgccatacgc tggcgcaggc gctgcatcgc ttctttaacg agcagggatt
     9241 cttctgggtt tcaacgccac tgattaccgc atctgatacc gaaggtgcag gcgaaatgtt
     9301 ccgcgtttct acgctggatc tggaaaacct gccgcgtaac gatcagggca aagtggattt
     9361 cgacaaagac ttctttggta aagagtcttt cctgaccgta tctggccagt tgaacggcga
     9421 aacctacgct tgcgcattat ccaaaattta taccttcggc ccgactttcc gtgctgaaaa
     9481 ctccaacacc agccgtcacc tggcggaatt ctggatgctg gagccggaag tggcgtttgc
     9541 taacctgaac gatatcgcgg gtctggctga agccatgctg aaatatgtct tcaaagcggt
     9601 tctcgaagaa cgcgcagacg acatgaaatt cttcgctgaa cgcgtagata aagatgccgt
     9661 ttcacgtctg gaacgcttca ttgaggccga tttcgcgcag gtggattaca ccgacgcagt
     9721 gaccattctc gaaaactgcg gcaggaagtt tgaaaacccg gtttactggg gcgtcgatct
     9781 ctcttctgag catgagcgtt atctggcgga agaacacttt aaagcaccgg tagtggttaa
     9841 aaactatccg aaagatatta aagcgttcta tatgcgcctc aacgaagacg gtaaaaccgt
     9901 tgcggctatg gacgttctgg ctccgggcat cggtgagatc attggtggct cccagcgtga
     9961 agaacgtctg gacgttctgg acgagcgtat gctggaaatg ggcctgaata aagaagatta
    10021 ctggtggtat cgcgatctgc gtcgctacgg tactgttccg cattcaggtt tcggtcttgg
    10081 ttttgaacgc ctgattgctt acgtaactgg cgtgcaaaac gtgcgtgatg tgattccgtt
    10141 cccacgtact ccgcgcaacg ccagcttctg attttcttcc tcaaagagcc agcgcctgct
    10201 ggctcttttt tatcctttct gtcaagttat ctgtttgtta agtcaagcaa tctatttgca
    10261 accccgccat aacgatcatc ctgttacgga atattacatt gcaacattta cgcacaaaaa
    10321 ataacccgca ttcttattgc ggattagttt tttcttagct gatagcacaa ttttcatact
    10381 attttttggc attctggatg tctgaaagag gattttgtgc caggtcgata aagtttcc
//
LOCUS       DAZOYS010000144        10379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOYS010000144 DAZOYS010000000
VERSION     DAZOYS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.144"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            178..1860
                     /locus_tag="ACHUN9_003139"
     CDS             178..1860
                     /locus_tag="ACHUN9_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0696473.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1912..3069
                     /locus_tag="ACHUN9_003140"
     CDS             1912..3069
                     /locus_tag="ACHUN9_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0696474.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3035..3157)
                     /locus_tag="ACHUN9_003141"
     CDS             complement(3035..3157)
                     /locus_tag="ACHUN9_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696475.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            3366..5051
                     /locus_tag="ACHUN9_003142"
     CDS             3366..5051
                     /locus_tag="ACHUN9_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR0696476.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            5089..7461
                     /locus_tag="ACHUN9_003143"
     CDS             5089..7461
                     /locus_tag="ACHUN9_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0696477.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7518..10301
                     /locus_tag="ACHUN9_003144"
     CDS             7518..10301
                     /locus_tag="ACHUN9_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0696478.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3058 a         2287 c         2254 g         2780 t
ORIGIN      
        1 tttatcggcg agaaattact taatagaaca gaaagtaatg tcaacgcttt atggactgtt
       61 atttcccttt ttttagctaa atctgctatc tctttatgtg actaacttca cttacatcca
      121 cttatttctc ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg aacatttatg
      181 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
      241 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
      301 gttgctttct cagattttac gccgactgaa tacagtacca aaggaaagcc aaatattatc
      361 gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
      421 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
      481 attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgt
      541 tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc
      601 ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg
      661 ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta
      721 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt
      781 gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc
      841 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
      901 ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
      961 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct
     1021 tataatgctc cgcacctacc aaatgataat cctgcaccgg atcaatatca gaagcaattt
     1081 aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt
     1141 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     1201 tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     1261 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     1321 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccaaca
     1381 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg tgtttccttg
     1441 ctgccctggt tgcaagataa gaaacaaggc gcgccacata aaaatctgac ctggataacc
     1501 tcttattctc actggtttga tgaggaaaat attccattct gggataatta ccacaaattt
     1561 gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc
     1621 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta
     1681 ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     1741 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt
     1801 agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa
     1861 ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc gatgcacgtt
     1921 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     1981 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc
     2041 aaacaatata tcgaagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     2101 actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg ctatgcaggc
     2161 caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt
     2221 gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc ccaggagtta
     2281 catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc
     2341 atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat taataacgtt
     2401 aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg
     2461 caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg tcatagtgag
     2521 aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca
     2581 accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa
     2641 agctttgtca gccgtttttt ggggaatggg catacaagtt gtattttcca ggagtcctgc
     2701 aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat
     2761 ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa
     2821 ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc
     2881 tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat
     2941 ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa cgccatggtt
     3001 gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa acaaatggag
     3061 aataattaat aatgctctct tttaactcca ttcttagcct ccttcttatg accttaacaa
     3121 ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc
     3181 ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc
     3241 ataaccataa ccactgaaaa ttaaaaattt ctatatattt catattgtta aatgatactt
     3301 aaataaattt catcagtaaa tcattgccat agataatcat tggatggcac aatcaatttt
     3361 cgctaatgat aaccattccc attacgtttt atatgttgat agcaaagtat ctctgtctgc
     3421 tgaagccctt ttggctgcgt aagaataata aaacttcagt gttgttaatc attattatcc
     3481 tcgcaatgat ccttggcgtg gttaaaattc aggtctggtt aaacgactgg aataatgatt
     3541 ttttcaatgc gctaagccag aaagaaaccg ataagctttg gcagcttgtt ctttggttcc
     3601 ctgccctgct ggggatcttt gtattaatat ccgtaaacaa aacatggtta attaaactac
     3661 tgactattcg ctggcgcgaa tggttaacag attactacct taatcgctgg tttgcagata
     3721 aaaactacta cttcacgcag atctacggcg agcataaaaa tacagataac cctgaccaac
     3781 gtatcgctga agacattctc ttactgatca gcaaaacgct cagtctttca tttggcttca
     3841 tccagtcact tagcatgctg atcaccttta ccgttattct ttggcaaagc gcgggtacgc
     3901 tctcattcac tgttggtgga acagaatgga atatccaggg gtatatggtc tataccgtcg
     3961 tgctcattgt gatcggtgga actttattta cccataaggt gggtaaacgt attcgtccgc
     4021 ttaatgtgga aaaacaacgt agcgaagcga catttcgaac taatcttgtg cagcataata
     4081 agcagacaga actgattgcg ctaagcaatg ctgagtctct acaacgccag gagttgcgcg
     4141 acaattttca taccattaaa gagaactggc atcgtttaat gaatcgtcag cggtggcttg
     4201 attactggca aaatatttat tcgcgctcgc ttagcgttct tccctacttt ctgttattgc
     4261 cgcagtttat tagcgggcag atcaatctgg gcggactgat gaaatcgcgc caggcattta
     4321 tgctagtatc gaacaattta agctggttta tttataaata tgacgaactt gctgaactgg
     4381 ctgcggttat cgatcgcttg tatgagttcc atcaactcac tgaacagcgc cctacgaata
     4441 agcctaaaaa ttgccaacat gcggtacaag tggctaatgc gagtattcgt acgcctgata
     4501 ataagatcat attagagaac ttgaactttc atgtttcgcc aggcaaatgg ctattactga
     4561 aaggctactc tggtgcggga aaaaccacac tgcttaaaac attatcccac tgctggccgt
     4621 ggtttaaagg tgatatttct tctcctgctg acagttggta tgtgtcacaa acaccgttaa
     4681 tcaaaagcgg cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata
     4741 aatcgttgag cgaagtactg catcaggttg gtctggggaa attggctgcg cgtattcatg
     4801 accacgatcg ctggggagat attctttcca gcggcgaaaa acaacgtatc gccctggcac
     4861 gattaatttt acgacgtccg aaatggatat ttcttgacga aactacctct catcttgagg
     4921 aacaagaggc tatacgctta ctgcgtttag tgcgtgaaaa actacccaca agcggcgtca
     4981 ttatggttac acatcaaccc ggtgtctgga acctggctga tgatatttgt gacattagca
     5041 cggttttata acaacaataa aagccctgct gtacggagat ataaataaat gaagcgagtt
     5101 cttattcctg gcgtcatttt atgtggcgct gatgtggcgc aggccgtcga tgacaaaaac
     5161 atgtacatgc atttttttga agagatgacg gtctatgctc ctgtccctgt acccgtaaac
     5221 ggcaacacgc attacaccag tgaaagcatc gagcgtttac cgaccgggaa tggcaatatc
     5281 agcgatctgc tgagaaccaa ccctgcggta cgcatggatt caacgcaaag tacctcgttg
     5341 aaccagggag atattcgccc tgagaaaatc tctattcacg gtgcgtcgcc ctaccagaat
     5401 gcctatttga ttgacggtat tagtgcaact aataacctga acccagcgaa tgagtccgat
     5461 gccagtagtg caaccaatat tagcgggatg tcacaggggt attatcttga tgtcagctta
     5521 ctggacaatg tgacgcttta tgacagtttt gtgccggttg aatttggtcg cttcaatggc
     5581 ggggtaattg atgcaaagat caaacgcttc aacgctgatg atagcaaggt gaaattgggt
     5641 tatcgcacta cgcgttcgga ctggttaaca tcgcatatcg atgagaataa caagagcgca
     5701 tttaatcaag gctcttcagg aagtacttat tactccccag attttaaaaa gaacttttat
     5761 accttgtcgt ttaatcagga actcgctgat aacttcggcg ttaccgccgg tttatcgcgc
     5821 cgccagtctg atatcacccg cgcggattat gtttcgaatg acggcattgt caccggtcgg
     5881 gcacagtata aaaacgttat cgatactgca ttgagcaaat ttacctggtt tgccagcgac
     5941 cgctttaccc acgatttaac cttaaaatat accggctcca gccgtgatta taataccagc
     6001 accttcccgc agtctgatcg cgaaatgggt aataaatcct atggtctggc atgggatatg
     6061 gatacgcaac tcgcatgggc caaactacgt accaccgttg gttgggatca tattagtgat
     6121 tatacccgtc acgatcatga catctggtac accgaacttt catgtacata tggtgatatt
     6181 acagggcgtt gtacctgtgg cggattagga cacatttccc aggctgtaga taattacacc
     6241 ttcaaaacac gcctggactg gcaaaaattc gccgtgggtg atgtttcgca tcaaccctac
     6301 ttcggcgcgg aatacatcta ttccgatgca tggactgaac gccataacca gtctgaatcc
     6361 tatgtgatta atgctgcagg aaagaaaact aaccatacca tttaccataa aggtaaaggc
     6421 agcctgggaa ttgacaacta cacgctgtat atggcggagc acattagctg gcggaatgtg
     6481 tcgttaatgc ccggtgtgcg ttatgactat gacaactatc tgtcaaacca caatatctcc
     6541 ccgcgcttta tgacggaatg ggatattttt gctgatcaaa cctcaatgat taccgccggt
     6601 tataaccgtt actatggcgg gaatattctt gatatgggat tacgtgatat ccgcaatagc
     6661 tggacggaat cggtatcagg taataaaact ctgacgcgtt atcaggattt gaaaacgcct
     6721 tataacgatg aactggcaat gggattgcag cagaaaatcg gtaagaacgt tattgcgcgt
     6781 gcaaactatg tttaccgtga agcgcatgat caaatcagca aaagcagtcg taccgacagc
     6841 gcgactaaaa ccaccattac tgaatataac aacgatggca aaaccaaaac gcattcgttc
     6901 agcctcagtt ttgaactggc cgaacccctg catatccgcc aagtagatat taacccacaa
     6961 attgtcttta gctatatcaa gagcaagggc aacttgtcgt taaacaatgg ttatgaggag
     7021 agcaataccg gtgataacca ggtggtttat aacggtaatc tggtctctta cgatagtgtt
     7081 ccagtggcag attttaataa cccattaaag atctccttaa acatggattt cacgcatcaa
     7141 ccgagcgggt tagtgtgggc gaatacgctg gcctggcaag aagcgcgtaa agctcgcatt
     7201 atccttggta agacaaatgc gcaatacatc agcgaatatt cagattacaa gcagtatgtt
     7261 gacgaaaaac tggatagcag cctgacctgg gacacccgct tgtcctggac gccacaattt
     7321 ctgaaacaac aaaacctgac gatcagtgcc gatattctca atgtactgga tagcaaaacc
     7381 gctgttgata caacgaacac cggtgtggcg acctacgcca gtggccgtac tttctggctt
     7441 gatgtcagca tgaaatttta atagttcgga ttttacttaa ccgggtaaat tcgcccggtt
     7501 ttcgcatgga gataattatg agaaacctct gtttcttact gacgttagtg gcaactctgt
     7561 tgctccccgg gcggctgatt gccgccgcct taccgcagga tgaaaagtta attaccgggc
     7621 aactggacaa tggcttgcga tatatgattt atccgcatgc tcaaccaaag gatcaggtaa
     7681 atttatggct gcaaattcat accggttcat tgcaggaaga agacaatgag cgcggcgtgg
     7741 ctcattttgt agaacatatg atgtttaacg gcacaaaaac atggccgggt aataaagtca
     7801 tcgaaacatt tgagtcaatg ggcctgcgtt tcggtcgcga tgttaatgcc tataccagct
     7861 atgacgaaac gctgtatcag gtgagtctgc cgactacgca gaaacaaaat ctgcaacaag
     7921 tgatggcaat cttcagtgaa tggagtaatg ccgcaacctt tgaaatactc gaagtagacg
     7981 ctgaacgcgg cgtaattact gaggaatggc gtgcccatca ggatgcgaaa tggcgcacct
     8041 ctcaggcgcg ccgccctttc ctgctggcaa atacccgtaa tttagaccgt gaacctatcg
     8101 gcctgatgga tactgtcgcc acggtcacac cggcacaatt gcgccaattt tatcaacgct
     8161 ggtatcaacc aaataatatg acctttatcg tggtcggcga tatcgacagt aaagaagcgc
     8221 tggcgctgat aaaggataat ttaagtaagc ttccggctaa caaagcagct gaaaatcgcg
     8281 tctggccgac aaaagccgaa aaccacctgc gctttaatat cattaatgat aaagaaaacc
     8341 gagtgaacgg catcgcactc tattatcgcc tgccaatggt acaagtgaac gatgagcaaa
     8401 gctttgtcga acaagctgaa tggagcatgt tagttcagct gttcaatcaa cgtctgcagg
     8461 aacgcataca gtcgggcgag ttgaagacta tttctggcgg cactgcgcgc agcgttaaaa
     8521 ttgcacccga ttatcagtcg ctgtttttcc gtgtaaatgc acgagacgat aatatgcagg
     8581 atgctgcaaa tgcattaatg gcagagttgg caaccattga tcagcatggt ttttctgctg
     8641 aagaactcga tgatgtcaaa tctacccgcc tcacctggct gaaaaatgcg gttgatcagc
     8701 aagctgaacg tgatttacgt atgctgacca gtcgcctggc atccagctca ttaaataata
     8761 cgccgttctt gtcgccggaa gagacatatc aactttcgaa acgtctgtgg cagcaaatta
     8821 ccgtgcaaag tctggcggaa aaatggcagc agttaagaaa gaaccaggac gcattttggg
     8881 agcaaatggt aaacaatgag cttgccgcca aaaaagcatt gtctcttgcg gctatcctgg
     8941 cgctggaaaa agagtacgcc aacaaaaagc tggcggctta catcttccca ggcagaaatt
     9001 tatcgttaac agtagacgct gacccacagg cggaaattag cagcaaagaa acgctggctg
     9061 agaatctgac atcattaaca ctttccaatg gtgccagggt tattctggca aaatccgcgg
     9121 gtgaagagca aaagctacaa attactgccg tatctaataa aggcgattta agtttccctg
     9181 cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt aagcggaagc ggcgttggtg
     9241 agctctcctc ttccagcctg aaacgctgga gtgcggaaaa ttcggtaacc atgagcagta
     9301 aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg gactaataac cctgaacctg
     9361 gttttcagtt gattaaccag cgaatcaccc acagcacgat taacgacaat atttgggcat
     9421 cgctacaaaa tgctcaaatt caggcgttga aaacgctcga ccagcgtcca gcggagaaat
     9481 tcgcccagca gatgtatgag acgcgctatg ctgatgaccg cacgaaatta ctgcaagaaa
     9541 atcagattgt acagtttact gccgcagatg cgctggctgc cgatcgccag ttgttttcat
     9601 ctccagcgga tatcacgttt gtcattgtcg gtaatgtctc agaagacaaa ctcgtggcgt
     9661 taattacgcg ttacttagga tcaatcaaac actctgattc gccattagcc gcaggtaaac
     9721 cattaactcg cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat gaacctgtgg
     9781 cacaggtttc acagtggaag cgttatgatt cccggacacc tgttaatctg gcgacgcgta
     9841 tggcgctcga tgcttttaac gtcgcactgg caaaagatct acgcgttaat attcgtgaac
     9901 aggcatctgg agcatacagc gtttcttctc gcctctcggt tgatcctcag gccaaagata
     9961 tcagtcattt gctggctttt acttgtcaac cagaacgaca tgatgaactg ttaacgttag
    10021 cgaatgaagt gatggttaag cgtctggcta aagggatcag tgagcaagaa ctgaatgaat
    10081 accagcaaaa cgttcagcgc agcctcgata tccaacagcg tagcgttcaa caattagcga
    10141 acactattgt aaatagtctt attcaatatg acgatcctgc agcatggact gagcaggagc
    10201 aattgttgaa acaaatgacg gtagagaatg ttaacactgc cgttaaacaa tatctttctc
    10261 atccggtaaa tacttatact ggagtattat tgccaaaata ataacagccc cgtcaacaca
    10321 tcgttggcgg ggattttagc aatattcgcc atttttatgt aataatttta taaatgcgt
//
LOCUS       DAZOYS010000145         9979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOYS010000145 DAZOYS010000000
VERSION     DAZOYS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.145"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..884
                     /locus_tag="ACHUN9_003145"
     CDS             <1..884
                     /locus_tag="ACHUN9_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0696479.1"
                     /translation="LRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGAR
                     LLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFI
                     RAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIV
                     HEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVI
                     TQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHE
                     QSPASRNFRIHLLNALR"
     gene            1019..2239
                     /gene="mlc"
                     /locus_tag="ACHUN9_003146"
                     /gene_synonym="dgsA"
     CDS             1019..2239
                     /gene="mlc"
                     /locus_tag="ACHUN9_003146"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0696480.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2364..3059
                     /gene="bioD"
                     /locus_tag="ACHUN9_003147"
     CDS             2364..3059
                     /gene="bioD"
                     /locus_tag="ACHUN9_003147"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0696481.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3012..4304)
                     /gene="clcB"
                     /locus_tag="ACHUN9_003148"
     CDS             complement(3012..4304)
                     /gene="clcB"
                     /locus_tag="ACHUN9_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0696482.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4463..5077)
                     /gene="dmsD"
                     /locus_tag="ACHUN9_003149"
     CDS             complement(4463..5077)
                     /gene="dmsD"
                     /locus_tag="ACHUN9_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0696483.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5120..5974)
                     /gene="ynfH"
                     /locus_tag="ACHUN9_003150"
     CDS             complement(5120..5974)
                     /gene="ynfH"
                     /locus_tag="ACHUN9_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0696484.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMTIAG"
     gene            complement(5976..6593)
                     /gene="ynfG"
                     /locus_tag="ACHUN9_003151"
     CDS             complement(5976..6593)
                     /gene="ynfG"
                     /locus_tag="ACHUN9_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0696485.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(6604..9027)
                     /gene="ynfF"
                     /locus_tag="ACHUN9_003152"
     CDS             complement(6604..9027)
                     /gene="ynfF"
                     /locus_tag="ACHUN9_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR0696486.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9088..>9979)
                     /locus_tag="ACHUN9_003153"
     CDS             complement(9088..>9979)
                     /locus_tag="ACHUN9_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0696487.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2447 a         2553 c         2590 g         2389 t
ORIGIN      
        1 aacttcgtca tctgcgttac tttgttgctg ttgcggaaga gctgcatttc gggcgcgccg
       61 ctgcccgcct gaatatttcg caaccaccgc taagtcagca gattcaggca ctggagcaac
      121 aaattggtgc ccgactgctg gcacgaacta atcgcagtgt attgctgacg gcagcaggaa
      181 aacagtttct tgcagatagt cggcaaatcc tgtctatggt agatgacgct gccgctcgcg
      241 ctgaaagact gcatcagggt gaagcggggg agttgcgcat tggttttact tcgtccgctc
      301 cttttattcg ggcggtgtcc gatacgttat cgctgtttcg ccgtgattat cccgatgtcc
      361 atttacaaac ccgcgaaatg aacactcgtg agcaaatcgc tccgctcatt gaaggaacgc
      421 tagatatggg attgctgcgt aacacagcgt taccggagac gcttgaacac gcagtcatcg
      481 tccatgaacc gcttatggcg atgatcccgc acgatcatcc cctggcaaat aacccgaatg
      541 taacgctggc tgaactggcg aaagaaccct tcgtcttttt tgatccgcac gtcgggacag
      601 ggctgtatga cgatattctc gggctgatgc gacgttacca tttgacgccc gtcatcactc
      661 aggaggtggg cgaggcaatg accatcatcg gtctggtttc cgccggtctg ggtgtttcaa
      721 ttttgcctgc ttcatttaaa cgtgttcagc tcaacgaaat gcgctgggtg ccgattgctg
      781 aagaggatgc ggtttctgaa atgtggttgg tctggccgaa acatcatgaa caaagtccag
      841 cttcgcgtaa ctttcgtatt catctgctga atgctctcag gtgagggaaa tttcagcgaa
      901 aaagcccgaa aaatgtgctg ttaatcacat gcctaagtaa aaatttgacg acacgtattg
      961 aagtgcttca ccatagccta cagattattt cggagcgcga aaatataggg agtatgcggt
     1021 ggttgctgaa aaccagcttg ggcacattga tcaaataaag cagaccaacg cgggcgcggt
     1081 ttatcgcctg attgatcagc ttggtccagt ctcgcgtatc gatctttccc gtctggcgca
     1141 gctggctcct gccagtatca ctaaaattgt ccgtgagatg ctcgaagcac acctggtgca
     1201 agagctggaa atcaaagaag cggggaaccg tggccgtccg gcggtggggc tggtggttga
     1261 aactgaagcc tggcactatc tttctctgcg cattagtcgc ggggagattt tccttgctct
     1321 gcgcgatctg agcagcaaac tggtggtgga agagtcgcag gaactggcgt taaaagatga
     1381 cttgccattg ctggatcgta tcatttccca tatcgatcag ttttttatcc gccaccagaa
     1441 aaaacttgag cgtctaactt cgattgccat aaccctgccg ggaattattg atacggaaaa
     1501 tggtattgta catcgcatgc cgttctacga ggatgtaaaa gagatgccgc tcggcgaggc
     1561 gctggagcag cataccggcg taccggttta tattcagcat gatatcagcg catggacgat
     1621 ggcagaggcc ttgtttggtg cctcacgcgg ggcgcgcgat gtgattcagg tggttatcga
     1681 tcacaacgtg ggggcgggcg tcattaccga tggtcatctg ctacacgcag gcagcagtag
     1741 tctcgtggaa ataggccaca cacaggtcga cccatatggt aaacgctgtt attgcgggaa
     1801 ccacggctgc ctcgaaacca tcgccagtgt ggacagtatt cttgagctgg cacagctgcg
     1861 tctcaatcaa tccatgagct cgatgttaca tgggcaaccg ttaaccgtgg actcattgtg
     1921 tcaggcggca ttgcgcggcg atctactggc aaaagacatc attaccgggg tgggcgcgca
     1981 tgtcgggcgc attcttgcca tcatggtgaa tttatttaac ccacaaaaaa tactgattgg
     2041 ctcaccgtta agtaaagcgg cagatatcct cttccctgtc atctcggaca gcatacgtca
     2101 gcaggccctt cctgcgtata gtcagcacat tagcgttgag agtactcagt tttctaacca
     2161 gggcacgatg gcaggcgctg cgctggtaaa agacgcgatg tataacggtt ctttgttgat
     2221 tcgtctgttg cagggttaac attttttaac tgttctacca aaatttgcgc tatctcaatt
     2281 tgggccagga aagcataact tagactttca aggttaatta ttttcctagt ttatatttgt
     2341 gaagcataac ggtggagtta gtgatgctgg agcgtttctt tattaccggt acagacactt
     2401 ctgtagggaa aacggtggtt tcccgcgcat tgctacaagc gttagcctcc cagggaaaaa
     2461 cagttgcggg atataaaccc gtagcgaagg ggagcaaaga gacacccgaa gggctgcgta
     2521 ataaagatgc cctggtgttg cagagtgttt caaccatcga actgccttat gaagcagtta
     2581 atcctatcgc gttaagcgaa gaagaaagta gcgtggcgca cagttgccca atcaattaca
     2641 ccctcatttc aaacggcctg gcaaacctga ccgataaagt cgatcatgtc gtggtagaag
     2701 ggactggcgg ctggcgcagt ctgatgaatg atttgcgtcc actctctgaa tgggtggtgc
     2761 aggaacagtt gccggtgttg atggttgtcg gtattcagga aggttgcatt aaccatgcac
     2821 tgctaacagc tcaggcgatt gccaacgacg ggctgccgct cattggctgg gtggctaacc
     2881 gaatcaaccc aggactggcg cattatgcgg aagtcattga tgtgctgggt aaaaaacttc
     2941 cggcaccgct cattggtgaa ctgccttatc tgccgcgcgc cgaacagcga gaactggggc
     3001 aatacatccg cttagctatg ctgcgcagtg tgctggcggt agatagagtc acggtgtaac
     3061 gtccgcgaaa ttaccgacgc aatgacgcag gcaatcaata aaccggggag tagctgatac
     3121 tccccggtca tttcacatat catcaacgtc gacataatcg gcgcgtgcgt ggttgccgcc
     3181 aacagtgtcg ccatcccggt caatccgagt aaaagcgtaa tttcttcgcc atcagggaac
     3241 cataatccca ggctacgacc atacaacatg ccaatggcaa gaccgataaa tagcgtcggt
     3301 gtaaagacac caccgggtgc gccggaaccg ctactcgcca gcacggcaca cagtttacaa
     3361 aggaagatcc cggcaatgat cattaacagt ggtggggcgg ttaaaaagga ttgtacggtg
     3421 ctatagccgt tgccccacac tgcaggtgtg aacagggaaa gcagacccac gatcaacccg
     3481 cctagtgcca gttgccaggg cggcgcaagt ttgagactca caaatccacg atgacaggcg
     3541 ttcattaacg ttaacaacag tggtccacac agacctgcca gcacacctgt actgataatc
     3601 agcgcatagt cactagcctg aaccgttact gagagttgta cgctgtagag taacgcgtcg
     3661 ctatgattaa taagattgct aaccagcaac gccacgacgg cggaaataat caccgggccg
     3721 agagaggcca acatcatagt gccaaacagc acttcggcta taaataaact gccagcaagc
     3781 ggggcgcgat aggccgcagc cattcccgcc gcggccccac aggcgatcca taatttccat
     3841 tcctggcgtg gcgtaaaacg ttgggcaaaa caggaggcgg caagggcagc taaaagaatc
     3901 atcgcacctt cgcgaccaat tgcactgccg ctggttacta ccagcagcga ggcaagcgat
     3961 ttaaccaggc ttgctgcgta atcgaactgt ccatcggttt gcaacgcttc catgtaatcg
     4021 gtcggcgcat gagggcgttg ttgggtaaat ttctgccagc ccatcagcaa caaacccgcc
     4081 gccagtccgc cgagcgccgg agttagcagc cgtcgccagg gggaaaggtt tgtcgctgca
     4141 ttgaccagac tgccggagtc attattgagg aacaaccact ccagtagcag catcgcatga
     4201 cgaaacccgg caacggcaaa ggccgcgaga ataccgacga ctgttgcgat aagcagacgg
     4261 cggaacatag tgcgcagatc cggatagatg tgtagatgat gcatgagcag aaatgggcaa
     4321 tgaaacacgg gcgactattt tgcggcaaaa gaggccaacc gcaaaagcag tgcgtgcttt
     4381 aagagataaa aaaacgtgac attgtaactt attattgccg aatcgcgcgt aatcgtcacc
     4441 atccggcaat attacggtga tcctatcgaa agagtggttt aaccgcgaca ggaatcaaca
     4501 gttgtgattg ccattgcgcc agcgttaagc gagccagttc acccagtgca cggtagaaag
     4561 ggtgttctgc tttttcgata aaaacatcaa gaaaacgtgt tgaccacgga aaaaggtgcc
     4621 atgccagcaa ttcttcgcat tctgtctggc gaccattctc cgccaaccac gcagccatca
     4681 acagcagtga accaaaatga tcttccggtt cgttttgctt catttcaaac tgaatgcctt
     4741 tctcgcgcat ccactgacga agtgccaatg ttgaatcgcc aaacagcaca gattcgcgat
     4801 ccagccagac cgatccccac ggcggagacg gcagtgccca cgggccgaca aacaaacgct
     4861 gccaggcctg ggcgtgagtc tcttcacact gtgtctgaaa agccgtcacc agcggcgcta
     4921 atgacgcttc tggtagaggc cactgagttt cccagccgtc actggtgagt accgcaacca
     4981 gaggtgcggc ttccgcactc tctggagcgt aataaaacag cgcgcccaac acgcgcgccg
     5041 cgacagaaaa attatcttgc tgtgaaaaat gggtcattcc taacatcctg aaatgtgcgg
     5101 gttgccccgc accaatttgt taacctgcaa tagtcattcc tactgtcatg tgcaggccat
     5161 aaaagagcac acgcccgatc atctcaccaa caagtatgag gattagcccc agaacaagtc
     5221 cggcaacgtg aggttcacgg cgacgaatga gcggacaaag ccagcatcca agacctgcgc
     5281 ataacaatac cacacgccag acctgtaagg aggcgtaatc tggtaccagt gcgctggctt
     5341 gttgcacgga tgagtgaata gacgccagag acaaaccttg cagcacaatc accccggcac
     5401 aagcgatcag cgcgagaacg ctaatgatgg caaatggtgt ggtattaaat gtaacgcggg
     5461 ctgcccgcag aattgcggca gcaagtattg gaccgctcaa caataccgtc aggaagaaag
     5521 ccagcgtagt gtaaccgtta tgccaggttg gcacagtgtc gatttgatac acgcaggtca
     5581 tcatccagac gaaaatgacg ccgagcgcca tgctgacaag taaccagagt ttccccaacg
     5641 cttgcggcat tttaccgatg accgccacca gccaccatag tccgccaacg gcaaaaaata
     5701 tggaaccggc agcaatctcg ttactcagac cagaagctcc gattcgattc agcgagttaa
     5761 acgcccgcag tggcgatccc aggtgcatga cagaggccat aaacccgatg cccatcaaca
     5821 gccagaggaa aaacatgcca cggacaatac gctgacggtc tgcgtcattt ttggcggcga
     5881 accagccaat tccgctaaca atcagcgcgc ctactacgca ttgacccaat acggtaaaga
     5941 taaccagtgg ccactcatgc catccatttc ccattttaca cttcctccgg attagccaga
     6001 taaccagtgg tatcgctggt cgggcggctg ttggcgttag gtttgataac gatattgggt
     6061 tttgtgaaat gcgcgcgcgg cagtggcgcg acggcagcca gagtgccgtg cttctggcgc
     6121 agctcttcaa ttggaccgaa ctcaagtgca cgcagcgggc aggattcgac acatatgggt
     6181 tgtttcccct cggcgacgcg cgaataacaa ccatcgcact tcgtcatgtg ccctttttca
     6241 gcattgtact gtggcgcgcc gtacggacaa gccatgtggc agtagcggca gccgatacaa
     6301 acatcttcat caaccaccac aaagccatct tcacgcttat gcattgcgcc gctggggcag
     6361 acttttgtac atgcggggtc atcgcaatgg ttacaggaga tggagagata ataagcgaac
     6421 acgttctggt gccagacgcc attatcttcc tgccagtcgc cgccagcata ttcataaata
     6481 cggcggaaac tgacttccgg gccaagatct ttaaaatctt tgcacgccag ttcgcaggtt
     6541 ttacagccag tacagcggct ggaatcgata aaaaatccat attgtgtggt cacgggctac
     6601 tccttaaacc ttttcgatct ggacaagatt gctgtgcgac gggtttccct ttgccagcgg
     6661 tgaagggcgg tgagaggtca gaatattgat actgccgccg tgatcgaccc ggtcaccaaa
     6721 catatccgct ttaagccaag caccttgccc gatggcggta acgccaggca gaatacgcgg
     6781 agtcactttt gcggcaatca gcatttctcc attattgtta aatacccgca cggtatcgcc
     6841 atgacggata ccgcgtgcct gagcatcaat ggggttgatc cacacctctt gtgggcaggc
     6901 ctgctgtaac acatcaatat tgccgtagct ggagtgggta cgcgctttgt aatggaagcc
     6961 cgttaactgc agtggatagg ttttccgcag gggatcgtcc cagccatcaa aatctggggt
     7021 atacgcagga aggggatgaa taacttcatc ttttttcaat tcccaggtat ccgcaatctt
     7081 cgccagtcgt tcagaataaa tttcgatttt ccccgaaggt gttttcaacg ggtttgcctg
     7141 tggatcttca cggaatgcgc ggaaagcgac gtagtgttct tccgggcatt ttttcttaaa
     7201 gatcccggtc gttttcatct cctcgtagtc gggcatctca gggttacgtt ccttcgtttt
     7261 cgcatggaga tatttgatcc attcatgctg actgcgacct tcagtaaagg tttgataaac
     7321 gtctggtcct aagcgtttgg cgacttcact cagcatccag tagatgggtt tgcgttcaaa
     7381 ttttgctgag gttgcgggtt gggcgaggat cacatagccc atattccctg cagattcatg
     7441 agagataagg tcttcttgct ctgttggcat caggtcgggc aacaggatat cgcaatactt
     7501 agccgaggcc gtcatgaagt ggtcaatgcc aacaatcatc tcgcacttgc tgtcatcctg
     7561 aagcacctca tgggtgtgat tgatgtcgcc atgttgattg atcaatgtgt tactggcgta
     7621 gcaccataaa aacttgatgg ggacatccag tttttctttt ccacgaacac catcacgggt
     7681 cgcggtcatt tccgtaccat ggtcgatggc atcagtccat gtaaagacgg aaatctgcgt
     7741 tttaacagga ttctcgagca tcgggaacca ttctaccccc agatcccagc taccttcgcg
     7801 tacgcctgag ttgccgccgt ttatgccgac gttaccggtg agaacggaaa gcatggcaat
     7861 agcgcgggat gtttgttcgc cgttggagtg tcgttgtggc ccccaaccct gacaaatata
     7921 agcaggtttt gctgaaccga tctctcgtgc caactggata attttttctg ccgggatgct
     7981 ggtgattttt gctgcccatt ccggcgtttt agctatgccg tcaggccctt cgcccagaat
     8041 ataggcttta taatgcgcgt tacgtggtgc gttggcgggc agcgtttttt catcgtaacc
     8101 aacacaatat ttgtcgagaa atggctgatc gaccatgttt tcagtaatca gtacccaggc
     8161 aatcgcacag gccagtgcgc catcggtgcc agggcgaatg ggcagccatt catcttcacg
     8221 cccggcagct gtgtcgttat aacgtggatc gatgacgatc atgcgtgcgt ttgaacgttc
     8281 gcgggcttgc tcgacgtagt aagtgacacc accaccgctc atccgcgttt ctgccgggtt
     8341 atttccgaac ataacgacca gtttcgtatt ggcgatatca tccgggctgt tgccatcatt
     8401 ggcaccgaac atataactca ttgcggcact gatctgtgcg gtactgtagc tgccatagcg
     8461 actgagaaaa ccaccgcaag agttcatcag acggtacggg acgtttgagt tggtgatgtt
     8521 tccgccatct acacctgttc cgtacaggac atgtacagct tcattgccgt aatcttttag
     8581 gatccgccga agattatcac tgatggtatc cagggcttcg tcccaactta tccgttcaaa
     8641 tttaccttca ccgcgcttgc cgacgcgctt catgggatat ttcaacctat caggatgatt
     8701 catccgtcgg cggatagagc gcccgcgtaa acacgcgcga acctgatgat taccgtagac
     8761 gtcgtcacct gtcgtatcat actccaccca gtacacggtg tcatctttca catgcaaacg
     8821 taacagacag cggctcccgc agttaacggt gcaggaactc cagaccgctt tctcttctac
     8881 cggagcctct gccgcccgaa ccatttggga aaatggcaga gtgaaagcac tgcttgccag
     8941 cgcaaggctg ccaagtgcgg aggttttcat cagacttcta cggctgattt cagccttcat
     9001 gagcgcctct gtggtatgga ttttcatcat tactcactta ttgcttttca aacaaaatgt
     9061 catgccagaa tttatggttg tagtgggtta tattttttcg atctcgacca gattagtgtg
     9121 ctgcgggttt cccttcgcca gtggtgaagg gcgcagagtg gttagcgtat tcacacagcc
     9181 gccatggtcg attttatcgc cagacatatt ggcctcgtgc caggctccct ggcccatagc
     9241 gctaacccca gggagaatgc gtggtgttac tttggctggt agccgaactt cgccacgatg
     9301 gttaaacacc cgcaccatat cgccggtggc aatcccacgt ttctgtgcat ctatagggtt
     9361 gatccacact tcctgacggc aggctgcctt caggacatca atgttgccgt aggtcgagtg
     9421 agtacgggat ttgtaatgga aaccaaacag ttgcagtggg aaggtactac gttcagggga
     9481 gtcccagcct tcaaaggttg aggcataaac tggcagtggg cttatcactt catctttttc
     9541 cagttcccag gtacgggcaa tttccgccag cttgctggaa taaatttcaa tcttaccgga
     9601 aggcgtttta agtggatttg cctcggggtc gtcacgaaat gctttgtagg cgacaaaatg
     9661 accattggga tctttacgct tatagatacc catttttttc agttcgtcgt aagacggtaa
     9721 cgccggatct ttggcaagca ttttggcgta cagatgttgt aaccattgtt cctgcgtgcg
     9781 accttcagtg aacttttgat agacgtcagg tccaagacgt ttcgcgactt cactcaggat
     9841 ccagtaaatc ggtttgcgtt cgaatttttc gctggtgaca ggctggagga aaatgagata
     9901 tcccatgtta ccggcgtagt cgttaggaat aatatcttcc tgctcaacgg tcatcaggtc
     9961 tggcagcaga atgtcggca
//
LOCUS       DAZOYS010000146         9819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOYS010000146 DAZOYS010000000
VERSION     DAZOYS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.146"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..154)
                     /locus_tag="ACHUN9_003154"
     CDS             complement(<1..154)
                     /locus_tag="ACHUN9_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696488.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNY"
     gene            232..1326
                     /gene="ddlA"
                     /locus_tag="ACHUN9_003155"
     CDS             232..1326
                     /gene="ddlA"
                     /locus_tag="ACHUN9_003155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0696489.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1350..1562)
                     /locus_tag="ACHUN9_003156"
     CDS             complement(1350..1562)
                     /locus_tag="ACHUN9_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0696490.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1822..2130
                     /locus_tag="ACHUN9_003157"
     CDS             1822..2130
                     /locus_tag="ACHUN9_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0696491.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2189..3283)
                     /gene="yaiW"
                     /locus_tag="ACHUN9_003158"
     CDS             complement(2189..3283)
                     /gene="yaiW"
                     /locus_tag="ACHUN9_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0696492.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3296..4516)
                     /gene="sbmA"
                     /locus_tag="ACHUN9_003159"
     CDS             complement(3296..4516)
                     /gene="sbmA"
                     /locus_tag="ACHUN9_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0696493.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4868..6025
                     /gene="ampH"
                     /locus_tag="ACHUN9_003160"
     CDS             4868..6025
                     /gene="ampH"
                     /locus_tag="ACHUN9_003160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0696494.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6026..6649)
                     /gene="iprA"
                     /locus_tag="ACHUN9_003161"
     CDS             complement(6026..6649)
                     /gene="iprA"
                     /locus_tag="ACHUN9_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0696495.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6737..9625)
                     /locus_tag="ACHUN9_003162"
     CDS             complement(6737..9625)
                     /locus_tag="ACHUN9_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0696496.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2536 a         2420 c         2358 g         2505 t
ORIGIN      
        1 tataattaag agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc
       61 cgttcatcat ttttgtgatc tcgcgcacat ctttgagata acgcactttc ttcaccagta
      121 cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg
      181 ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa
      241 ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg
      301 gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt
      361 gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat
      421 cctgcccata ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat
      481 gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc
      541 ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc
      601 aatttaccgt ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc
      661 accaaacgtc tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc
      721 gctaatcgtc acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt
      781 gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag
      841 tacgcaattg ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg
      901 atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc
      961 tgtggcgaga tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa
     1021 gatggcgcga aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg
     1081 gcgattgccg ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg
     1141 tttttaaccc cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc
     1201 aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc
     1261 acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca
     1321 atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga
     1381 cgacgaataa tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca
     1441 taggtctgcc aaccttttgc ttcaaatccc agtaacccca ccacgccatt aagaatgaat
     1501 ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc
     1561 atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt
     1621 gtagacaaca ttttcgtaat tgttaataaa tttaacttaa ggttgccatt ttgtaactca
     1681 caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg
     1741 acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc
     1801 agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga
     1861 aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt
     1921 tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag
     1981 cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc
     2041 accattttcg ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta
     2101 tggctgcact ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg
     2161 ccttatctgg catgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt
     2221 ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt
     2281 gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat
     2341 aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac
     2401 ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg
     2461 ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc
     2521 cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt
     2581 taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt
     2641 aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca
     2701 ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc
     2761 caccggtgcg caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga
     2821 tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc
     2881 tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa
     2941 tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa
     3001 cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt
     3061 tttccagcgt cggtgccagc ccctgacttt caaaggtgat ggcgagatct ttcgcccagg
     3121 catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg
     3181 ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa
     3241 ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc
     3301 tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca
     3361 gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga
     3421 aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg
     3481 taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat
     3541 agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc
     3601 gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac
     3661 cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg
     3721 gcagtttgat ccctaccact gccagccatc cggtccccat cagcgaccag acgatagcgg
     3781 caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga
     3841 gcgttaccag caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc
     3901 ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac
     3961 cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc
     4021 agcggaacac atagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca
     4081 gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg
     4141 gcgaacttag cgcggtttga atcaaatcat agaacggtgc ataccaggcg ttgacggcga
     4201 ccccgacttc caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc
     4261 agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa
     4321 caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg
     4381 ggatctgccc ggaagcgccg gtgatacgcg ccacccagtc tcccccaccg gcttgccaga
     4441 agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgctccc ggctttggga
     4501 aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta
     4561 ttaaccgcat gaccactaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc
     4621 aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa
     4681 gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag
     4741 gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc
     4801 tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac
     4861 accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc
     4921 cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta
     4981 tgccgatcat attttttacg gcagcggtgc aacggggatg gcgctggttg ttatcgacgg
     5041 taaccagcgc gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca
     5101 gctggattcg gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt
     5161 gaaattgctc gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc
     5221 aggcgcacgc gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca
     5281 taccagcgcc ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg
     5341 gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg
     5401 cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc
     5461 gtccggcaag ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa
     5521 agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg
     5581 tgccagtccg tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc
     5641 tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa
     5701 ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg
     5761 catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa
     5821 agagggtcgt ccggggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat
     5881 ggcgatgatc ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac
     5941 gcgctttaaa aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa
     6001 accgttggtt atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca
     6061 gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga
     6121 cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc
     6181 gcaattcttc attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat
     6241 tatgcccaat gagctgcacg tcgcgtaatt ccagaattct attttgccag gctaaccagt
     6301 aaaaagcgtc tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta
     6361 gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta
     6421 aaccatcagc cagccccata atataagggg cctgggtaat accgataagt acgttttctt
     6481 ctctacgcag agagataact ccctctaaaa taacgaaagt atcttcgtta ttgacatcac
     6541 tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac
     6601 atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct
     6661 ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc
     6721 ttcggagtga agaatattac caggtatatt taacacccac gttcgcggac cagtcttgat
     6781 ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc
     6841 tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat
     6901 caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg
     6961 gagtcagagc ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat
     7021 aacgcatgct gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat
     7081 caccagactg gaacagacca gaaacgctgc cgtaaggagt cacgtaacca gcatcaccca
     7141 gtttgaagtc gtaaccggct ttcaagccga agccccaagc gtcggagttg gtgctaccgt
     7201 caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca
     7261 agctaccatc aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct
     7321 ggctgtcttg atctacctga ccagaacggt cattcatgtc acctttagcg aagcctgcag
     7381 ccgcaccgac gatccactta gcgttgttac cgtcaacttt ggtatcaaca ccgaccatga
     7441 tgccgttaac atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga
     7501 agtagcttac ccatgcgccg ccgttatcag ccaggccatg acgagagttg gtcagacgag
     7561 taccaacggt gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagcg
     7621 ccatgttagc gtagtcggtc agctccatct gttgcagaac aacggtgtta ccgcgctgtt
     7681 cagcctgata ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgg
     7741 ttttggtatc atcgatatag atgagctgct tacctgcgta atcagcaatc gcaccagcgc
     7801 cagtggagtt gtcgatacga actttgtagt taccggttgc agctacaacg ttgtctgcag
     7861 agctgttgtc aacttcgtcg ccattgttaa tgtcgttgtt accattaacg gtcaagtgac
     7921 catcagagtt catcgcaata acaccataac cgtagttagc tttgctggtg tcgttggtac
     7981 gatcgttgac gaggtctgca ttcaggatgt aatcattact atgaatgtca aacacacctg
     8041 cgtaaacatt accgttgtta tctttaacgt tgccactggt cagttccaga gtatcagttg
     8101 caaatacgct ggagatgtca ttcatgttgg tgtttacatc cagcacacca ccgttagtaa
     8161 cggtaatggt attggcatac agcggtgcat atgcccaagc tgaagcagta tcttcaccga
     8221 agttaacggt tgagaaggta tcaatggtca ggtgatcggt gttaacttca ccgttaactt
     8281 ccattgaaga accaccgttc agagtgatgg tgtcagaagt cagagaagaa tctgcactca
     8341 cgttaacctg agaaccgctg ttgatcgcca gggtatcgat cagggaagtt ttcgtggttt
     8401 cccaatcaga accgttgttc agaacaacat cgaatacgcc gctctggaat gcgttagcgt
     8461 tatcaacacg attccagcca tcttcagagt aagaagcgta cgggttcagg ctgtttggtg
     8521 caacgtcgta gaaggcttcg tcagcaacaa caacgtcata atcaatattc gctgaaccaa
     8581 cccatttggt gttgttcaga gtcaggttca ggtgatcgac gttctgatcg tcagttgccc
     8641 aaccgccatg agtgtaagca gtggtagagt tgtcaatcag gtgacccgtc gcgtcaaagt
     8701 tagcgttcca ggtactgtca aatacaacgt caccaagcaa ctgagagttg ttgaacgtaa
     8761 cgttgttgac catacgataa tcagaagtgt catcatcgat gaaggctaac gcaacatcat
     8821 cagcaggaga tgcgccattg ccgtagtcac ttggttcaac agaattgccg aaatgaccac
     8881 gatctgattg ttcgtcttca gtccatgaac cagaagttac agtagagtca ttaacaacga
     8941 tgttgttgtc ccactgatga ccttcggtat tgtaggtcgc attaccgagg tcgtgttgaa
     9001 ccaaagtaat acctgcaaca tgggagttgt tgctgatgtt gatgttactt tcaacatcca
     9061 gagtcacagc ggtaccaaga ccagcggtta ccaaacgatc ataatctgtc ttagatgttt
     9121 taccgtctgc atccagataa gtatcggtga agtagaaggc gtcaaaatca tcgtcaatag
     9181 tagaattagc gatgttcaga gtgaagatgt cgccatcaac ccagtcgtgg ccagtataat
     9241 cgctaccgtt ggtccagacg tagtcgccat aaccaacctg gtaagatgta accataccat
     9301 gaacaattga gttggagatg ttcagtgtat taccggtatc tgtgccataa tcgtggttca
     9361 gataataagt agaaattaca ccatttacag tgtcgccgct gaaagtggta tcaaagtaac
     9421 catcagctgc gtggtcatat acataaccac cgtaagtcag ataatcgtga tcaattttag
     9481 tgctgtcacc ccaattgtag atggtggctg cgtcatcgtg atacccgaag gtgttataag
     9541 tagctgcatt agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta
     9601 caagtttctt tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc
     9661 catcaattat caatgcatag aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta
     9721 cagacgaatg gttcaattat tctttcccct aagtccgaat gtggacatat ataatttatg
     9781 gagaaaaaca cgcatcaaat tgataaaagg acgatattt
//
LOCUS       DAZOYS010000147         9786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOYS010000147 DAZOYS010000000
VERSION     DAZOYS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.147"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHUN9_003163"
     CDS             complement(104..418)
                     /locus_tag="ACHUN9_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0696497.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHUN9_003164"
     CDS             complement(423..638)
                     /locus_tag="ACHUN9_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0696498.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHUN9_003165"
     CDS             complement(789..2642)
                     /locus_tag="ACHUN9_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0696499.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHUN9_003166"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHUN9_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHUN9_003167"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHUN9_003167"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHUN9_003168"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHUN9_003168"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHUN9_003169"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHUN9_003169"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHUN9_003170"
     CDS             complement(3908..4966)
                     /locus_tag="ACHUN9_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0696500.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHUN9_003171"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHUN9_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..6360)
                     /locus_tag="ACHUN9_003172"
     CDS             complement(5539..6360)
                     /locus_tag="ACHUN9_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0696501.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(6357..6737)
                     /locus_tag="ACHUN9_003173"
     CDS             complement(6357..6737)
                     /locus_tag="ACHUN9_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0696502.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(6738..7796)
                     /locus_tag="ACHUN9_003174"
     CDS             complement(6738..7796)
                     /locus_tag="ACHUN9_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0696503.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(7798..8076)
                     /locus_tag="ACHUN9_003175"
     CDS             complement(7798..8076)
                     /locus_tag="ACHUN9_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696504.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            complement(8115..8403)
                     /locus_tag="ACHUN9_003176"
                     /pseudo
     CDS             complement(8115..8403)
                     /locus_tag="ACHUN9_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8624..8779)
                     /gene="hokD"
                     /locus_tag="ACHUN9_003177"
     CDS             complement(8624..8779)
                     /gene="hokD"
                     /locus_tag="ACHUN9_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0696505.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(9071..9415)
                     /locus_tag="ACHUN9_003178"
     CDS             complement(9071..9415)
                     /locus_tag="ACHUN9_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696506.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(9412..9579)
                     /locus_tag="ACHUN9_003179"
     CDS             complement(9412..9579)
                     /locus_tag="ACHUN9_003179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696507.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            complement(9590..>9786)
                     /locus_tag="ACHUN9_003180"
     CDS             complement(9590..>9786)
                     /locus_tag="ACHUN9_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696508.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
BASE COUNT         2312 a         2739 c         2236 g         2499 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ataaaatttc ttaactgttt tttcccatga
     5641 cgcgcgtgtt atctggtttg tcacctcgca tatagcatta aatgcctccg ttgatggtag
     5701 tctttcatag ccacgaccac cacaacgctg gcagtctctg ataacaggca taccacgttt
     5761 taccgactct tcacgatgaa tggcgacacc acgcccacgg caatccttac aggcggtgga
     5821 aacctcaccc tttccgccac actccggaca ggcaactttt accacctccc tgactttttt
     5881 ccattcttcc cagtaagacg gatacacacc tttcgtacac tttgcccata ccggcggctt
     5941 accatccgga tactggatct tgtttgtaaa aacctcgctt tcaataaatt tttttccgtg
     6001 acagcagggg cactgttttt tgctcgccgc gctacgggca taatcttcaa acgcatacga
     6061 agccataata cgcatcactg ccggttttat ttctgccggg agttttctta acgccgccac
     6121 gcgatcacac cgactgagtg catattctgt cagcaattct gttgcccgct ctctgtcatt
     6181 catactaatg cccattttcc caaggaacgc agaaaccccc atctcagccc gattctgtgt
     6241 catgccctgc gcggccatca catcagtgat actcagcgca tctttcgacg ttgaggccga
     6301 tgcatcagtc aggccggggg attttgggga gtagtatttc ggtaaatctt ccagtttcat
     6361 tttttgacct gcccttcaag cattatggga taaatcttca cccccagacg tccaccagat
     6421 actggctgag cacgaacgat attgatttca tcaaactgct catcgtccat tagcaacccc
     6481 gcatgcgtca gcgcatccag cggcgctttc agaatattgt ccaggtcacg gcggcgctta
     6541 tccggtggtt ctgcaataat ttttattgcc aaccttccgg acaggcttaa tttcagccgc
     6601 tgctggcgaa caataagcgc cactgcccgg cgataacgct ccccggcttt tgatacaaaa
     6661 tatgtgctgc cacgacgacg ccagtaagtg ttcaccgtcg gcgggtaagg taaaacaaat
     6721 tctatgcgct cggtcattta tgctttccac ttcagaacac ccgaatttct cgcgtgcatt
     6781 aaaaaacgaa tcagcaacaa cagctgactg ccgtgtttct cttcaaaatc ttttactccg
     6841 gcatgtagtt cgttacggca tttacggcat aacggaataa caaacaaatc atcagccttt
     6901 gttcccatcc ctcccagtcc atgaccaatg atgtgatgcg gatcatctgc ctgattgcca
     6961 cacgtcatgc atttctgcgt ttttacccag cgcgtgtata caggcatctc ttcccgttgt
     7021 gatttctggc gctggagata ctgagccggt gactccggat caacggcaat gctgaccacc
     7081 gtcttttcct gtggtgggtt ctgttgctgg tggacgtgag gcagtaacgc aatatttttt
     7141 gtgcgctgct tcagtatgct ggtggctgtc tgctctcccg gtatgatgtc gctttcactg
     7201 tacacagagc gaattttttc cgcacgcaac cccagcgaac gacgtaatac cgcttccggt
     7261 agcgcgtccg ccacctgatt gcggaccgcc caccaggata attcagccag agataattca
     7321 cgctcctgcg taccgcttat tgcgtgaccg atgacgtcaa tcatccatgc tgacaggttt
     7381 tgatgagcaa gttgctcgag tgattcggat gtctggtcac gcagctggtt gtcgcagtgc
     7441 cagcacaaca ccattgcgcc ggtaccataa cggtgaatga cggtttcgct gtgatgataa
     7501 tcgccgtgtg gccactggca ggatttaata tggcgcaaca gccagtcaga caatgcacca
     7561 gcaccaccag cagcacgaat cacccgtgcg ttactgaaaa acggcagcaa tgttttgtct
     7621 tccaccagcg gctggcgagc ggcaggaacg accccggacg gcagattacg catgcttttt
     7681 ggttccggct ccaccagtat tctgccgtta tggaatgctg acattgattc acggcctggc
     7741 ttaacgataa ccagaccgag ttccggtacc agaacaggtc gaagtaatac ccgcacatta
     7801 cctccagatc cgttgctgga atgtgcggga cggacgcgtt ggccgttcgg agtaagggag
     7861 cctgacggag attatccagt gacggtagtc gaggctaagg gcttttttaa cctcgcatcc
     7921 gcgcctgcgg taacactgaa tgagccattc ggcctgttct tcagtgcatg ggggatgctg
     7981 gtaccaatca gatttaaatg cgtgagagcg ccgcccgtgc ctgctggcaa agacggctga
     8041 attatcagaa ttgtgtagtc tggaattttg cgccatcggc tttctccggt ggcacagtgt
     8101 tactcaacag gggttcagcc ctgcgctgaa ttgtagatga attcactcat cttcaaaagc
     8161 agaaaaacca gccttaatcc cagcttcttt cagagacggc aacgatgtga caaattcatt
     8221 tgcacgcgaa ataaaaccat ccgtcacaag cccatccaca aaatgaatta acgcagctcc
     8281 actcttcctt tgttgagact gtaaacattt aatacggcag tggctgacaa tagcgccatt
     8341 ctcaacgcgc acagtataga ggccatcttc actaaaaatt tcacgtaatt cttcgatttt
     8401 catcaacaga atccttccag ataaatagca ctcccctgtt cggggtccat ccctcttctc
     8461 cctgcgcgct acttaagtat ttttgattct attccggcac catctaaaac ttcaaacgcg
     8521 ttgaaaataa aaacaaaaac ccgccgaagc gggttaagtg tgggtgcatt gaggatgcct
     8581 gacccatcag aggtggcgag ggatttctcc cccgcctggt ctcttactcc tcaggttcgt
     8641 aagctgtgaa gacagcgacc tccgtctggc cggttcgaat tcgtacctcg cagaggtctt
     8701 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattaaca
     8761 tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcacgtaa gaggctaacc
     8821 tagatttgcc gttcatagat tgagcctcag attaatgtta agcgtcttgc cggacgcgta
     8881 atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaga cagatagcct
     8941 caagcacccg ctgcaattct acttaactct ccttttcccg caaaccgttt ttatccccag
     9001 cggcaaatcg aatacaccac cagcgccacc gccatcgcaa ttcctaccgt tgttaatgct
     9061 tcaggccagg tcatcgtaaa atatcctcca cgcttatcag tccgttccgc tccagataac
     9121 tcatcgcctt atccggtaat ttgcagtctg gcttcgcttt tctcagttgc caggttaact
     9181 gctttaccag catggttaac tcatcgacca gacgctgata tcccactggt ttgtattcat
     9241 gcaatttacc ggctggctct gctgccagcg ataccagtgc gatttccaga acagcaatat
     9301 ccatcttata tgtgcggatg atgtcatggt cgattgtgcc cggtatgcac agtctctgtg
     9361 cttcaatagt ctcctctgcg tgagctatta actgctctct ggtaaaagtc gtcatgccgt
     9421 agccccttct tgatattttt caaaccagaa cacaaccggc tctgcttcca gcgatgccag
     9481 cgcaatccgt gccagttcca tttgttcacc acgggtaagc ccgttttcaa gcgggttttt
     9541 aatgaacaat tcaatacgtt ctttggtaat agtggtcatg tgttactcct taacccgcag
     9601 tgctttcaac tgatgagggg aacaaaatct tttcatcaaa ccctgcattc atatcatgaa
     9661 cagcaacaca ccaatccatc gacgaacgat tatcaagagc ctccatgatt tcatccatgc
     9721 ggcgtaggtc atacaggtaa atgcttttat cgccaatggt gtaaaagcca atttttttcg
     9781 gtgatg
//
LOCUS       DAZOYS010000148         9757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOYS010000148 DAZOYS010000000
VERSION     DAZOYS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.148"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            430..2061
                     /gene="oppA"
                     /locus_tag="ACHUN9_003181"
     CDS             430..2061
                     /gene="oppA"
                     /locus_tag="ACHUN9_003181"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0696509.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2147..3067
                     /gene="oppB"
                     /locus_tag="ACHUN9_003182"
     CDS             2147..3067
                     /gene="oppB"
                     /locus_tag="ACHUN9_003182"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0696510.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3082..3990
                     /gene="oppC"
                     /locus_tag="ACHUN9_003183"
     CDS             3082..3990
                     /gene="oppC"
                     /locus_tag="ACHUN9_003183"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0696511.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4002..5015
                     /gene="oppD"
                     /locus_tag="ACHUN9_003184"
     CDS             4002..5015
                     /gene="oppD"
                     /locus_tag="ACHUN9_003184"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0696512.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5012..6016
                     /gene="oppF"
                     /locus_tag="ACHUN9_003185"
     CDS             5012..6016
                     /gene="oppF"
                     /locus_tag="ACHUN9_003185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0696513.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6069..6398)
                     /locus_tag="ACHUN9_003186"
     CDS             complement(6069..6398)
                     /locus_tag="ACHUN9_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0696514.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6433..7893)
                     /gene="cls"
                     /locus_tag="ACHUN9_003187"
     CDS             complement(6433..7893)
                     /gene="cls"
                     /locus_tag="ACHUN9_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0696515.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8036..8209
                     /locus_tag="ACHUN9_003188"
     CDS             8036..8209
                     /locus_tag="ACHUN9_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0696516.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8264..9517)
                     /gene="kch"
                     /locus_tag="ACHUN9_003189"
     CDS             complement(8264..9517)
                     /gene="kch"
                     /locus_tag="ACHUN9_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0696517.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSKLDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2557 a         2277 c         2422 g         2501 t
ORIGIN      
        1 aagatagaca tgcttagcca atctctattt gattgaattg aaagatgttt gttaaggcat
       61 ggatgcaagc tatagattct gatacggtca ataaaagaga attgcttaac aattttgcaa
      121 aatgtattgg cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca gacgattctt
      181 tattggtaat gagaataatt aacaattaaa gagcgtcgcg aaagaataat gtgtctcgac
      241 aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga ataaataaag
      301 tcggtgatag taatacgtaa cgataaagta acctgacagc agaaagtctc cgagcctgtg
      361 cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa tgagggagtc
      421 caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt tctggctgcg
      481 ctaatggcag ggaatgtcgc gctggcagct gatgtacccg caggcgtcac actggcggaa
      541 aaacaaacac tggtacgtaa caatggttca gaagttcagt cattagatcc gcacaaaatt
      601 gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact ggtcagcgat
      661 cttgacggtc atccagcacc tggcgtcgct gaatcctggg ataataaaga cgcgaaagtc
      721 tggaccttcc atttgcgtaa agatgcgaaa tggtctgata gcacgccagt cacagcacaa
      781 gactttgtgt atagctggca acgttctgtt gatccgaaca ctgcttctcc gtatgccagt
      841 tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa aaaaccgatt
      901 accgatctcg gcgtgaaagc tattgatgat cacacattag aagtcacctt aagtgaaccc
      961 gttccgtact tctataaatt acttgttcac ccatcaactt caccggtgcc aaaagccgct
     1021 atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa cggtgcctat
     1081 accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc gacctactgg
     1141 aacaacgcga aaaccgttat taaccaggta acctatttgc ctattgcttc tgaagttacc
     1201 gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt ataacaacat gccgatcgaa
     1261 ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc atacctgtgc
     1321 acttactatt acgaaattaa caaccagaaa ccgccattca acgatgtgcg tgtgcgtacc
     1381 gcactgaaac taggtatgga ccgcgatatc attgttaata aagtgaaagc gcagggcgac
     1441 atgcccgcct atggttacac tccaccgtat actgatggcg caaaattgac tcagccagaa
     1501 tggtttggct ggagccagga aaaacgtaac gaagaagcga aaaaactgct ggctgaagcg
     1561 ggttataccg cagacaaacc gttgaccatc aacctgctgt ataacacctc tgatctgcat
     1621 aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt aaacgtcaaa
     1681 ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggcac ttttgatgtg
     1741 gcccgtgcag gctggtgtgc agactacaac gaaccaactt ccttcctgaa caccatgctg
     1801 tcgaacagct cgatgaatac cgcgcattat aagagcccgg cctttgacag cattatggcg
     1861 gaaacgctga aagtgactga cgaggcgcag cgcacagctc tgtacactaa agcagaacaa
     1921 cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc gcgtctggtg
     1981 aaaccgtggg ttggtggcta taccggcaaa gatccgctgg ataataccta cacccggaat
     2041 atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc tccacggtgt
     2101 ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt taaaatttat
     2161 tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta tttcgttctt
     2221 tatgatgcgc ctcgcgccgg gaagcccttt taccggcgaa cgtactttac cgccggaagt
     2281 gatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac agtatttcag
     2341 ctacctgaaa caactggcgc acggtgattt cggtccatcg tttaaatata aagattattc
     2401 ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc aaactgggag ccgccgcatt
     2461 tttccttgcg gtaatactgg gtgttagtgc tggcgttatt gtcgcattaa aacaaaacac
     2521 caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc ccagttttgt
     2581 ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc cgggcggtgg
     2641 ctggaatggt ggggcgctta aattcatgat attgccgatg gtggcgttgt cactcgctta
     2701 tatcgccagt attgcgcgta ttacccgagg ctctatgatt gaagtattac actccaactt
     2761 tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac gccacgcatt
     2821 aaaacctgct ctgttacccg tgctctctta tatgggcccc gcatttgtcg gcattattac
     2881 cggttctatg gttatcgaaa ccatttatgg tttgccgggg attggacaac tgttcgttaa
     2941 tggcgcattg aaccgtgact attccttagt gttaagcctg accatcctgg ttggtgcttt
     3001 aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc cgaaaatccg
     3061 ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac gctggaaaat
     3121 ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg tcgacgtttt
     3181 atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc gttatttgta
     3241 atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc gatgatgtcc
     3301 agcgccccgg atatggagtc cggtcactac tttggtactg actcatccgg tcgcgacctg
     3361 cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc tgcggcactg
     3421 gtggcagtgg tcgtggggac actttacggt tcgctttccg gttatctggg cggtaaagtg
     3481 gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat gttcttcgtc
     3541 attttgcttg tgaccttttt cggtcaaaac atcctgctga ttttcgtggc gattggcatg
     3601 gtttcctggc tggatatggc tcgtattgtg cgtggacaaa ccctgagtct gaagcgcaaa
     3661 gagtttattg aggcggcaca agttggcggt gtatcgacat cgggcattgt tattcgccac
     3721 attgtgccga atgtactcgg tgtggtggtg gtctacgcat cacttctggt gcccagcatg
     3781 atcctctttg aatctttcct tagcttcctg gggttgggta cgcaagagcc gttaagcagc
     3841 tggggtgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg gttattgttg
     3901 ttcccagcgg gattcctcgt ggtgacgctg ttttgtttca actttatcgg cgatggcttg
     3961 cgtgatgctc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta attgaaactg
     4021 caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa gatttgcgtg
     4081 tcacctttag taccccggac ggcgatgtca cggcggtcaa tgatttgaat ttttctctac
     4141 gtgccggaga aacgctgggc attgtaggtg agtctggttc gggtaaatcg caaactgcat
     4201 ttgcgttgat gggcctgttg gctgccaacg gacgtattgg cggatcggca accttcaatg
     4261 ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa actgcgcgct gaacaaatct
     4321 caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc ggtgagcagt
     4381 tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt gaagagtcgg
     4441 tgcggatgct cgatgcggta aaaatgccgg aagcacgtaa acgcatgaaa atgtacccgc
     4501 acgaattttc tggtggcatg cgtcagcgag ttatgattgc gatggcattg ctatgtcgac
     4561 ctaagctgct gattgcggat gaacccacta ctgcgctgga cgtcaccgta caggcgcaga
     4621 tcatgacgct attaaatgaa ctgaagcggg aatttaatac cgccatcatt atgattaccc
     4681 acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac gccgggcgca
     4741 cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgttcatcct tattctatcg
     4801 gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga aacaatgttg accatccctg
     4861 gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca cgttgtccgc
     4921 atgcgatgga aatttgtagt agcgcaccgc cgctggaaga gtttacgcct ggccgtctgc
     4981 gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag gaagaaaagt
     5041 cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca aacagtggtt
     5101 ctggcaaccg ccgaaaactc tcaaagccgt cgatggtgtc acgcttcgcc tgtatgaagg
     5161 ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg ctcgcgccat
     5221 cattggcctg gttaaagcga ccgacggtca tgttgcctgg ttaggtaaag agttgctggg
     5281 catgaagccc gatgaatggc gtgccgttcg cagtgatatt cagatgattt tccaggatcc
     5341 gttggcatcg ctaaacccgc gtatgaccat cggcgagatc atcgctgaac cactgcgtac
     5401 ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga tgatgctgaa
     5461 agtcgggtta ttgcctaacc tgattaaccg ctatccgcat gagttctccg gtgggcagtg
     5521 ccagcgtatc gggattgctc gtgctcttat tcttgaaccg aagctgatta tctgcgatga
     5581 gccgatttcg gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc tccagcagct
     5641 gcaacgtgag atgggattgt cattaatttt tatcgctcat gacctggccg tggtaaaaca
     5701 catttccgat cgtgtgttgg tgatgtatct cggccatgcg gtagaactgg ggacctatga
     5761 tgaggtctac cacaatccac tacatcctta caccagggca ttgatgtcgg cagtccccat
     5821 acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg aattaccgtc
     5881 gccgatcaac ccgccttccg gttgtgtttt ccgtacctgt tgcccgattg ccggtccgga
     5941 gtgcgccaaa acacgtcctg tgctggaagg gagtttcaga cacgccgttt cttgcctgaa
     6001 agtcgatccg ctttaaacac aataagggct gacagttgtc agcccttttt cacgccgaaa
     6061 gcgattattt attcccgcca gatgatatgg caaagtttgt gatctttttc gcggcataac
     6121 agaatgcggg cgaaaacatc gttgatttca ccatcctcac tgtccgccag accaatcacc
     6181 acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat gttcctgcca gtcttccgcc
     6241 ggatcgaata actccgcgcc gccgcgctct tcaaactgaa gattgaacag cagaacatca
     6301 gctgggtcga gattgtcggc agccagttcg agaaaaatat cgtaggcctg ctcaagcgtt
     6361 tcatcttcag tcaggcgatt gtttagatcc atatccatag tcactacctg tttaacctct
     6421 gttggcgacg ttttacagca acgggctgaa gaagtaaaac agtcgctcgg cgacacgttg
     6481 ccataatgga cgttttagcc ataaacgggc atcgagcaga cgtgaacgcg aaatataatc
     6541 gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg tcgattgcca gggtaatctc
     6601 gaaattcagc cacagactac gcatatcaag gttaacggtg ccaaccagac ttagttcgcc
     6661 atcgaccagc acgctcttgg tatgcagtaa cccgccttca aactgataaa ttttaacccc
     6721 agcagcaagc agttccgtaa agaatgcgcg actggcccag ccgaccagca tcgagtcatt
     6781 ttttcgcgga aggataatac tgacatccac cccgcgctgc gccgccgtgc aaatcgcatg
     6841 aagtaaatca tcgcttggca caaagtaggg cgttgtcatg atcaaatatt cacgcgccga
     6901 ataagccgca gtcaataatg cctggtgaat gagatcttcc ggaaagccgg ggccagaagc
     6961 aattgtgtga atggtgtgac cgctggcctg ttcaaacggc ataatattga catctggtgg
     7021 tggcggcaga atacgttttc cggtttcaat ctcccagtcg caggaataaa taatccccat
     7081 cgcggtggcg atggggcctt ccatacgcgc catcagatca atccattgcc ctacgcccgc
     7141 atcttgtttg aagtagcgag gatcgaccat attcatgctg ccggtgtacg cgatgtaatt
     7201 atcgatcatg atcatcttgc gatgttggcg caggtccata cggcgtaaaa acacacgcat
     7261 cagattgacc tttaaggctt cgaccacttc aataccggca ttacgcatta gctcgggcca
     7321 cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc atcaatcggc aatgaatacc
     7381 acgccgtgca gccgccatta atgattcagc cacctggtcc gccatgccgc cgggctgcca
     7441 gatataaaac accatctcaa tattatggcg cgcgagctgg atgtcgcgga ttaacgcctg
     7501 catcacatca tctgactcgg tcatcagttg tagctgattc cctttgaccc cagcgatccc
     7561 ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca ctgctatttt cttcggcgaa
     7621 gatatgctta caggctttaa ggtcgttaag ccattttgcg gtggaaggcc acatcgctct
     7681 ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca acggcaagat aggcaataat
     7741 tccgactaac ggcagaatgt aaataatcaa cagccaggcc atcgcggagg gaactgcgcg
     7801 tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc aaccagtatc ccagaatggc
     7861 caaccaactc accaacgtat aaacggttgt catagattag aaatcctttt gaaagcgcat
     7921 tgttatgagt ttacgcatat ggattcatct ggcaaataaa aacgcgggaa aagcactggt
     7981 cagcgtcggg ggtgggggta taatgaccat tctgttattg catagagtag ttaacatgaa
     8041 gcggagtaga acggaagtgg ggcgctggcg catgcagcgt caggctagcc gacgtaaatc
     8101 gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc cacagcatca ggaagtgcat
     8161 tctaaacaaa cagcgtaact cgttattgtt tgcgatctac aatatctaaa tatactgggc
     8221 accattgcgg tgcccaataa gtttactctt tcgtctttaa actctatttt tgcactgatt
     8281 ctttactgtc agctttcgtt tcttgttcat cgttatcgct gaaaatgcca tgaccggagt
     8341 tcaacaacat tgaaacgagc atatcattat taatctcttc accatttaaa actcgcgcca
     8401 gaatttcgct gccaaacagt tgcggtgaga gaatgatatc cggatgtacc atcttaatct
     8461 tattcaggtt tttgctatca ctgacggcga gaactgtttt gacatcactg ctcatatctt
     8521 ttgccgagag tacaacaaac gcgttatctg catcgttatc actcagcgcc agaatggccc
     8581 ggcaacgatc gattcccgct ttctttaata ctgaactgtc attactgtca ccggggataa
     8641 catcagcgtt atcgcctaag cgttgctcaa gttgcttgat atcatcttca ggcaagttgc
     8701 tgataaccgt tacgttttgt ccgcgttgat tcagttgcag gatcgtattg atggcgagaa
     8761 tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat tgtatgattg tttcctttta
     8821 caagtttgtt gaatcccccg cggataagcg gaccaaaaat tgaggtcata gatgtggcaa
     8881 aaacggtaat gccggaaata atgaccgaaa tagtgaacaa tcgtgctgat tcagaaacag
     8941 ggacaatatc gccatagccg acggttgaca tggtttctat cgaaaaatag aacgcggtca
     9001 tcaaactttc tattcgcgga ttaaaacctt cgcttaaata aagcgcaccg taggttgagt
     9061 aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt cccggctgcg gcgctactgt
     9121 gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt aaaaatgcaa aatccaatac
     9181 taaatttcag ccagggataa aaatgcaggg tgtaaattag cgctatcaac aacaaaatga
     9241 tactgattgc ccatgcgagc tttgcccgga acagtaagcc aatggagttc aggacaagaa
     9301 agacgccgag cataaatagt ggggcgtttg ccagcaagga gagatccaac tttgaaaatg
     9361 ctttgatatg gaaaatatca agcaaattga ccgacatacc atagattgtt ttaaaaataa
     9421 gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg ccgaagggtc acccataaat
     9481 ttgttgctgt ttgtttgaat gtagcccagt gactcactga tacctctgta accacaattt
     9541 tgtttaatta tcttagcgtg cacattttct gaacatacat gcagcgcgtg aatgtgttaa
     9601 acaatagtag atgactttat ccgcagtaat caaaggattt gagcgagcaa ctgtacctca
     9661 ggaaaggtca tttttcctga atatgctcac atcatataaa gaaatacaga taaagttatt
     9721 atctgcttgt ggtagtgaat gcactgaccg gctataa
//
LOCUS       DAZOYS010000149         9674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOYS010000149 DAZOYS010000000
VERSION     DAZOYS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.149"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUN9_003190"
     CDS             <1..1710
                     /locus_tag="ACHUN9_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0696518.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUN9_003191"
     CDS             1711..3360
                     /locus_tag="ACHUN9_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0696519.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUN9_003192"
     CDS             3365..4141
                     /locus_tag="ACHUN9_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0696520.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUN9_003193"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUN9_003193"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0696521.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUN9_003194"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUN9_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0696522.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUN9_003195"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUN9_003195"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0696523.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUN9_003196"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUN9_003196"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0696524.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIKQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUN9_003197"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUN9_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0696525.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2483 c         2605 g         2232 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgcttg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZOYS010000150         9645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOYS010000150 DAZOYS010000000
VERSION     DAZOYS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.150"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUN9_003198"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUN9_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0696526.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUN9_003199"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUN9_003199"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0696527.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUN9_003200"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUN9_003200"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0696528.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHUN9_003201"
     CDS             complement(5698..7014)
                     /locus_tag="ACHUN9_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0696529.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHUN9_003202"
     CDS             complement(7004..8761)
                     /locus_tag="ACHUN9_003202"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0696530.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="ACHUN9_003203"
     CDS             complement(8777..>9645)
                     /locus_tag="ACHUN9_003203"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0696531.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAZOYS010000151         9621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOYS010000151 DAZOYS010000000
VERSION     DAZOYS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.151"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHUN9_003204"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHUN9_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHUN9_003205"
     CDS             2192..3328
                     /locus_tag="ACHUN9_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0696532.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHUN9_003206"
     CDS             3325..5325
                     /locus_tag="ACHUN9_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0696533.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="ACHUN9_003207"
     CDS             5450..5911
                     /locus_tag="ACHUN9_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0696534.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="ACHUN9_003208"
     CDS             complement(5952..6422)
                     /locus_tag="ACHUN9_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0696535.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHUN9_003209"
                     /gene_synonym="yehT"
     CDS             complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHUN9_003209"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0696536.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHUN9_003210"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHUN9_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0696537.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="ACHUN9_003211"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="ACHUN9_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9385..>9621
                     /locus_tag="ACHUN9_003212"
     CDS             9385..>9621
                     /locus_tag="ACHUN9_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0696538.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2354 a         2531 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg ctgacgagtg g
//
LOCUS       DAZOYS010000152         9590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOYS010000152 DAZOYS010000000
VERSION     DAZOYS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.152"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            773..1459
                     /locus_tag="ACHUN9_003213"
                     /pseudo
     CDS             773..1459
                     /locus_tag="ACHUN9_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1792..2667
                     /locus_tag="ACHUN9_003214"
     CDS             1792..2667
                     /locus_tag="ACHUN9_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0696539.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2675..3451
                     /locus_tag="ACHUN9_003215"
     CDS             2675..3451
                     /locus_tag="ACHUN9_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0696540.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            3620..5881
                     /locus_tag="ACHUN9_003216"
     CDS             3620..5881
                     /locus_tag="ACHUN9_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0696541.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5950..7125
                     /gene="senB"
                     /locus_tag="ACHUN9_003217"
     CDS             5950..7125
                     /gene="senB"
                     /locus_tag="ACHUN9_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0696542.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7651..8112)
                     /locus_tag="ACHUN9_003218"
                     /pseudo
     CDS             complement(7651..8112)
                     /locus_tag="ACHUN9_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(8150..>9590)
                     /locus_tag="ACHUN9_003219"
     CDS             complement(8150..>9590)
                     /locus_tag="ACHUN9_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0696543.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVRQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYA
                     DDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESY
                     LRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT         2547 a         2337 c         2298 g         2408 t
ORIGIN      
        1 aaaattgacg ccagactaat tattaataat ccagttatct ttttattcat atcatctcct
       61 ttccattggt taatttatcg gaaattcatc cccactaact aaaacgatcc acacaataaa
      121 actaaatcta attgcaggca atcaattcca gtcgatatat catctgtatg gaaatcatcg
      181 ctcatcactc atcgcagcct gaaagcccag agcaacgggg caggtctgca tataagtggt
      241 tacacggcag aattccagag ggttctgtca ctaaattcag tcactgacaa atttgcatga
      301 tgttgaatag acgagatttt gtgtcgaaat agttgtttca gtaactgtca ctcaatcggt
      361 ggtttatgtg actatattcg caaaaactgg tcgcagaaaa gtcctgtttt cagcttggaa
      421 acccggtaat cagaataatg gataggggaa caaaaatctc tgtatagaac gctccattga
      481 gggagagaaa acgtgtcatt ttgacccgtt cggtattcag aacacacaaa accgatcgcg
      541 ggtcgctttc gtgcatagtt atgcaacgcg ctaaaaacga ttctgacgca ttttttatgt
      601 ttaccctgtt ttttcttgtc tttttgagtt ttttgcgtca gagagcgtct gagagggcac
      661 gtacaacggg agttatggta aatggatcgg gttttcggga aggttcgaca ggatttgccg
      721 ttgggtgtag tgtaagcgac tgaaaaacaa acgcccctga aatcatgtcc agatccggca
      781 gaaccagcct catgcgcagt tctggctggt ttttgacgtt gatcgcgagg gggcagcgat
      841 tgactggagc gaccggaacg ctcccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg
      901 tcatgctcac ctgctctacg cgctcaatat cgccgtgaga accgcgccgg acagctcggt
      961 taaggcgctg aaatacgccg ctgcgattga acgttcgctg tgtgagaaac tttgtgctga
     1021 tgtgaactac agcggactga tttgtaaaaa tccgttccac ctggaatggc aggtgatgga
     1081 gtggcgagag gaagcctata ccctcgatga actggctgat tatctcgatt tgagcgcctc
     1141 agcgcgccgt agcatcgata aacattacgg gatggggcga aactgccacc tgttcgaaat
     1201 gacgcgcaaa tgggcttaca gggcgattcg tcagggctgg cccgaattct cacagtggct
     1261 tgatgccgtg attcagcgtg tcgaaatgta caacgcatcg cttcccgttc cgctttcacc
     1321 gcctgaatgt cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc
     1381 ggaaactttc gcacagtatg tggctgatac gcacacatta acgtacgttt tcgttccatt
     1441 ggccctcacc ccccgataac gacccaggta gcgcgccgta ttccccccgc cggccgtttt
     1501 cttcgacatg tacatcaggc gcggaactca tcggggcgcg ggaatgaaat cccattcaga
     1561 ctccggatag attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaacgggagt
     1621 gaccatagat ccgttttcca agcggacccc ataatatcgg ctggtaaaca taactcgcta
     1681 gtgcattctt tttgttggta atgataatca tttacattca ttctttgatg ttttatcata
     1741 attaatgttt tgtcaataaa tatatggaaa catattgttt cagatgggaa tatgagaaag
     1801 ttcatcctta tttctatgat aacgttagct agtgttagtc tgggagcctg cagacagaat
     1861 gtcaccataa agcaggatgc gccagggcag aaagctttcc tgactgacgg gcagatttac
     1921 gatttgcaca gtggcaaaat tatttctagc agcgagcttt tggcggatct tgctaccgct
     1981 cgacatctta ttattggtga aaaacacgat aatgccgagc atcatcagat tgagctttgg
     2041 cttattcaaa atttgctcat ccagcgacca caggggagtg tgttgcttga gatgttgacg
     2101 tcagagcaac agccccgagt taaccaagtt aaatgttggc tgaaagataa tcctgtggtt
     2161 cgagatagcc gtgttcagga actacttaac tggcaaaaag ggtggtcctg ggagatgtat
     2221 ggcgacatcg tcatgcagct attacgtggg ccttacccgc tgctcaatgc caacattggc
     2281 cgggagcaga tgttggcact gtacaaaaaa aacgaatttc ctaaaggaaa aaaatccact
     2341 gcacctgtgg tacaagaagc tctgcgtgaa accatcatat ctatgcatga aggcaacctt
     2401 gaaagccagc aactgacgtc aatgctctct attcagcagc agcgggatcg ctacatggcc
     2461 cgccagcttc tgagtgcccc ggtaccgtcg ttgttgattg caggtggtta tcacgcgtca
     2521 aaaagcatgg gcgtaccgtt acacatggaa gatcttgcca ccggcaccca tccagtggtg
     2581 ttgatgcttg cagaaaaagg aatgaacatc acagtagatc atgctgacta cgtgtggttt
     2641 gtagcgccag atacaacaaa aaggtaaacc atggatgatg aatattctcc acttctcaca
     2701 gtcggtaaaa tggagcagct ggtttatctg ttctctactg cttcatggac tgattttcct
     2761 tgcgtttatc tggcgttttt ccgaagttca gcccgctatg tctcctgcac cggcgatcat
     2821 gctgcaatgg gcagaaatta tagaggctcc ttcttctcca ctttcgcttc cggtgggaat
     2881 cgctcaacag gagtccgcag tgacagaaga aaagcaacaa actgaggaca gacaacaacg
     2941 tcctgtcact gaagacagtg atgcaaccat tgaaataact aggaagaaaa agtcatccga
     3001 tggagaaaag aaaaagacac gtccacctcg taagataaag gctcaaacca gcgacagcaa
     3061 ccccacagcc gtttccagta atgctgcacc tcaggctctg gtcgaatcgt cccgtattgc
     3121 tgcgcctttt aacagtgact caacaaagcg agataattct gaggcatctt gggaaagcag
     3181 agtaaaaggc cacctaaacc gttataaacg ttaccctggc gatgccagaa aacgtgcacg
     3241 aaccggcaca gccgtcgtga cttttaccgt aaacacggaa ggcactattg tcagtagttt
     3301 cttggaaatc tcttcaggaa ccctttctct tgaccgcgaa gctattgctg ttttagaacg
     3361 cgctcaacca cttcccaaac caccgcctga aatattggag ggaggattat ttaaagtaaa
     3421 aatgccgatt acctttaagt tgaaggagta aagggataat tggccgttca agttcattta
     3481 actgaaattt aattttcatt tttatgctga acattttatc aaacttttcc actgtcgaaa
     3541 aaatcaaaaa gacagtagaa cataattaat aaacaaatag ttatacttat ctttattaat
     3601 attataccca aggaaattta tgaacgttat aaaactggct atcggctcag gcatattatt
     3661 gctcagctgc ggtgcttact cacaatccat cagtgaaaaa actaattccg acaaaaaagg
     3721 agcggcagaa ttcagtccgc tcagcgtttc tgtcgggaag acgaccagtg agcaggaagc
     3781 tctcgagaaa acaggcgcga ccagttcccg gacaacggac aaaaacctgc aatcacttga
     3841 cgcaacagtg cgtagtatgc ctggtactta tactcaaata gatcctggtc agggagcaat
     3901 cagtgtgaat attcgaggca tgagcggatt tggtcgtgta aacactatgg tcgatggtat
     3961 tacccagagt ttttacggaa cctctacctc cggaacaacg acgcatggtt caactaacaa
     4021 tatggctggc gtacttatag atcctaactt actggtagca gttgatgtta cacgcggtga
     4081 cagcagtggc tctgaaggga tcaacgccct tgccggtagt gcaaatatgc gtactattgg
     4141 cgttgacgat gtaatattta acggtaatac atatggcctt cgttcacgtt tctctgtcgg
     4201 tagtaatggg ctgggacgca gcggaatgat cgcccttggt ggaaaaagcg acgcttttac
     4261 ggatacggga agcattggcg ttatggctgc tgtgagcggc agttctgtgt actctaattt
     4321 ctcaaatggt tctggaatta acagcaaaga gtttggttat gataaatata tgaagcagaa
     4381 ccccaaatcc caactgtata aaatggatat cagaccagac gaatttaaca gcttcgaact
     4441 ttccgctcga acctatgaaa ataaatttac acgtcgtgat ataaccagtg acgactatta
     4501 cattaaatat cattacaccc ctttttctga attaattgac tttaacgtaa cggccagtac
     4561 cagtcgcggt aatcaaaagt atcgtgatgg ctcgctgtat actttctaca aaacctcagc
     4621 gcaaaatcgt tctgacgcgc tggatatcaa caataccagc cggttcactg tcgcggacaa
     4681 tgacctggag tttatgctgg gcagcaaact gatgcgtacc cgctatgacc ggaccattca
     4741 ctcagcggcg ggcgacccga aagcgaatca ggaatcgatc gagaacaatc cgttcgcacc
     4801 ctccggccag caggatattt cagcgctgta taccgggctg aaggttacgc gcggcatctg
     4861 ggaggcagat ttcaatctca actacacacg taacaggatc acagggtaca agcccgcctg
     4921 cgattcacgc gttatctgcg tgccacaggg tagctacgat attgacgata aagagggtgg
     4981 cttcaaccct tcagttcagc tttctgctca ggtaacacca tggcttcagc cgttcattgg
     5041 ctacagcaaa tccatgcgcg ccccgaacat ccaggagatg ttcttctcta attcaggagg
     5101 cgcatccatg aacccattcc tgaagcctga acgtgcagaa acctggcagg cgggttttaa
     5161 cattgatacc agagatttac tggtcgaaca ggatgccctg cgctttaagg ctctggcgta
     5221 ccgcagcagg atccagaact acatctacag cgagtcttat ctggtttgtt ctggaggtcg
     5281 taaatgcagt atgcctgagg tgattggcaa tgactgggag ggcattagcg atgaatacag
     5341 cgacaatatg tacatctacg ttaactcggc aagcgacgtt atcgcaaagg gcttcgaact
     5401 ggagatggat tatgatgcag gttttgcttt tggccgactc tctttcagcc agcagcaaac
     5461 agaccagcca acctccatcg ccagcaccta ctttggcgca ggggatatga ccgaactgcc
     5521 cagaaaatac atgacgctgg atactggtgt tcgcttcttc gataacgcgt tgaccctggg
     5581 cactatcata aaatacacag gcaaggctcg tcgcctgtcg cctgattttg agcaggacga
     5641 acataccggc gcaataatca aacaggattt gccgcagatc ccaacgatta tcgatctcta
     5701 tggtacttac gagtacaacc gcaacctaac actgaaactt tcggtacaaa acctgatgaa
     5761 cagagattat tcggaggcgc tgaataagct caacatgatg ccaggtcttg gtgacgagac
     5821 ccacccagcc aattccgcgc gtggcagaac atggatattt ggcggggaca ttcgtttctg
     5881 atgacaattt ccgctaggtt cagacaatac gttttctctc ttatgtcaat tttattgcag
     5941 gaacgaaaaa tgaatatttt cactttatcc aaagcaccgc tatacctgtt aatttcacta
     6001 tttttaccca cgatggccat ggctatcgat ccacctgaac gcgaactttc gcgatttgcc
     6061 ctgaaaacga attaccttca gtcccctgat gaaggcgtct atgaactggc gtttgataat
     6121 gccagtaaaa aggtgtttgc agcagtcacc gatcgtgtaa atcgtgaagc caataaaggc
     6181 tatctgtatt cgtttaattc agattcgctg aaagtcgaaa ataaatacac gatgccatac
     6241 cgggcatttt cgctggcgat aaatcaggat aaacatcagc tctatatcgg acacacccag
     6301 tcagcgtccc tgcgtatcag tatgtttgac accccaaccg gcaaactggt aagaaccagc
     6361 gacaggttaa gttttaaagc ggcaaacgct gcagattcgc gttttgagca ttttcgccat
     6421 atggtttaca gccaggattc cgataccctg tttgtgagtt atagcaatat gctgaaaacg
     6481 gccgagggca tgaagcctct gcataagctg ttaatgctcg acgggacgac gcttgcctta
     6541 aaaggcgagg ttaaggatgc ttacaaaggt acagcgtatg gtctgacgat ggatgaaaaa
     6601 acacagaaaa tctacgttgg cggaagagat tacatcaacg aaattgatgc gaaaaatcag
     6661 acgctgctgc gtaccatccc gttgaaagat ccgagaccac aaatcacaag tgtgcagaat
     6721 ctggcggtgg actccgcttc tgaccgtgcc tttgtggtgg tattcgacca tgacgatcgt
     6781 tccggtacaa aagatggact ctatattttt gacttacgcg acggtaaaca gcttggctat
     6841 gtgcacacag gagccggagc taacgcggtg aaatacaatc cgaaatataa cgaactgtat
     6901 gtcaccaact tcactagcgg caccatcagc gtagtggatg ccaccaaata cagcatcacc
     6961 cgtgaattta acatgccggt ctacccaaac cagatggtgt tgtcggacga tatggatacc
     7021 ctttacattg gcatcaaaga aggctttaac cgcgattggg atcctgatgt gtttgtggaa
     7081 ggagctaaag aacgtattct gagcattgat ttgaaaaagt cgtgatcggg gggaaaaaac
     7141 atgggataaa aactccgtta ttccactaaa ctggccggac taggaaaagc aaacatctct
     7201 gtcctgccgg tacgcctgtt acgccgctcc atgaggctga ggaatctcca gaaaacgaga
     7261 caggcacagc attgtacgat caactgattt gtgccaataa ttaaatgaaa tcacagctat
     7321 gcctgttcat ggggttctag gccggaaccc cagatttttc cgattgtttg ttttctaact
     7381 gattattcag tagttttaga gggctgacgc tcagtggaac gaaaactcac gttaagaggt
     7441 tttgactatg ataaaagtca aagtaggggt gagtttggat atggaaatta ttgtactata
     7501 aggagagcat gaaaacccct ggggcagtca ctgaatgagg gaccagcagc ggtaaaataa
     7561 ccagcataat ttctgcatgg tcgattttta taccgtgaca ggctgttttt tatacttggg
     7621 gtcagtttgg atatcgaaaa ttattgtacg ttaaggttgt tttttgacct tgtcgatctt
     7681 aactgatctc aaacgacgaa acttacctga agtcggtatc cggctgtttt tccagatata
     7741 atcgcctgtc agattgatat gttcccaacc taatggtgat aaatgcgata atagttgagc
     7801 atttattttc atgcccttac gcttcagagc atcaaccgct ctttccatgt aaactgtgtt
     7861 ccataaggaa atagccgccg tcaataaagt caatccactg gcgcgataac tttgattttc
     7921 cggtttccga tctctaatct cccccaatcg atgcatgaat acagcacggg ccagagcatt
     7981 acgggcttct cctttgttca gcccggcctg gacacgtcgc cgcaatgccg gatcccgaaa
     8041 ccagtccagc ataaacagtg ttcgttctat acggccaatc tctctcagag ctttagccaa
     8101 cccattttgt ttgtaagcgt gcagcaagaa ccgtattgac gggaatgtgt tattcagtcg
     8161 gcagtgctac gcgccagggg agcagttcgc cgacccggtt tatcggccag tcggctatga
     8221 cgtcaaggac atagcggagg tagctttctg gctccactcc gttcagtttg cacgtcccga
     8281 tcaggctgta cagcagcgct ccccgctctc ctccatgatc cgaaccgaag aacaggtagt
     8341 ttttgcggcc cagactgacc atccgcaacg cattttcagc gatgttattg tccgcctcag
     8401 cccagccatc atctgcatag tacgtcagcg ccggccactg gttcagggcg tatgcgaacg
     8461 ctttcgccag ttctgagtgt cgcgacaggg ttttcatctt ttcacgcagc cagctttcca
     8521 gggatttcag cagcggtttc gttttcaact gacgttcggc aaggcgctgt tccgccgtca
     8581 ttccccttat ctctgcctcg atggcgtaca gttcgccgat ccgtttcagc gcttcctccg
     8641 tcagggctga cggggtgcga acgtgcacat cgtggatttt acggcgggcg tgagcccaac
     8701 aggcggcttc cgttatccgg ccatcccggt acagctcgtt gaacccggcg tatgcatccg
     8761 cctgccgtac accactgaac cccgcaagat gggtctgcgg atggatgcct tttctgtccg
     8821 ggctgtaagc gaaccacacc gccggcgcca gcgttgaccc ggcgttacgg tcgtcacgaa
     8881 catacgccca caaccgcccg gtcttcgttt tcttattgcc tggcaacagc accgggacag
     8941 gcgtgtcatc aacatggagc ttaccgtcag tcagcacata gccatgaagc gcctcttcca
     9001 tcggagacag cagccggcag catgcatcca cccagcccga cagcagtgaa cggctcagct
     9061 ccacaccttg ccggccgtat atttctgact ggcgatacag cggggtgtgc tctgcatact
     9121 tcgaggtcag cacgcgggcc agcagccccg gtccggcgat accccgctcg atgggccgcg
     9181 aaggtgcagg tgcctgcacg atggcatcgc actgagtaca ggcatgtttt tcccgtaccg
     9241 tccggataac ccggaaggcg ctacgcatca actccagctg ttcggcggta tcctcgccca
     9301 gatagctcag tgaaccgccg cagttcgggc agcacggcgc cgcaggcaac agtcgctttt
     9361 cgtcacgggg tagtgattca gggaacggct tacgggtgcg ggtctgacgc aacggacgct
     9421 gtactgccgg gtcatacacc ctaccagtca gcgtatcgct ctctttctga agccggttca
     9481 gatcggcttc catttgtgcg atacggcggg agactttttc ggaacgactg ccgaagttca
     9541 tccggcggag tttatccagc tgcgcctgca gatggtctat ttcgcgctcc
//
LOCUS       DAZOYS010000153         9577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOYS010000153 DAZOYS010000000
VERSION     DAZOYS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.153"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUN9_003220"
     CDS             complement(<1..163)
                     /locus_tag="ACHUN9_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0696544.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUN9_003221"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUN9_003221"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0696545.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUN9_003222"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUN9_003222"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0696546.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUN9_003223"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUN9_003223"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0696547.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUN9_003224"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUN9_003224"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0696548.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUN9_003225"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUN9_003225"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0696549.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUN9_003226"
     CDS             5058..6191
                     /locus_tag="ACHUN9_003226"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0696550.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUN9_003227"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUN9_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0696551.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUN9_003228"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUN9_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0696552.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUN9_003229"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUN9_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0696553.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUN9_003230"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUN9_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0696554.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUN9_003231"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUN9_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0696555.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUN9_003232"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUN9_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0696556.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2429 a         2459 c         2460 g         2229 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttactc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaatta
//
LOCUS       DAZOYS010000154         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOYS010000154 DAZOYS010000000
VERSION     DAZOYS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.154"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUN9_003233"
     CDS             122..952
                     /locus_tag="ACHUN9_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0696557.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUN9_003234"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUN9_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0696558.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUN9_003235"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUN9_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0696559.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUN9_003236"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUN9_003236"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0696560.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUN9_003237"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUN9_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0696561.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUN9_003238"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUN9_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0696562.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUN9_003239"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUN9_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR0696563.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUN9_003240"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUN9_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR0696564.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUN9_003241"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUN9_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0696565.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2260 a         2483 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaacca gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZOYS010000155         9427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOYS010000155 DAZOYS010000000
VERSION     DAZOYS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.155"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUN9_003242"
     CDS             <1..173
                     /locus_tag="ACHUN9_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0696566.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHUN9_003243"
     CDS             complement(345..674)
                     /locus_tag="ACHUN9_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0696567.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHUN9_003244"
     CDS             complement(679..864)
                     /locus_tag="ACHUN9_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0696568.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHUN9_003245"
     CDS             complement(861..3500)
                     /locus_tag="ACHUN9_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0696569.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUN9_003246"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUN9_003246"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0696570.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUN9_003247"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUN9_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0696571.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHUN9_003248"
     CDS             complement(5227..5493)
                     /locus_tag="ACHUN9_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0696572.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUN9_003249"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUN9_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0696573.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2345 a         2461 c         2305 g         2316 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatata
//
LOCUS       DAZOYS010000156         9402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOYS010000156 DAZOYS010000000
VERSION     DAZOYS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.156"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHUN9_003250"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHUN9_003250"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0696574.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHUN9_003251"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHUN9_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0696575.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHUN9_003252"
     CDS             1394..1915
                     /locus_tag="ACHUN9_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0696576.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHUN9_003253"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHUN9_003253"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0696577.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHUN9_003254"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHUN9_003254"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0696578.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHUN9_003255"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHUN9_003255"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0696579.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHUN9_003256"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHUN9_003256"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0696580.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHUN9_003257"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHUN9_003257"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0696581.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHUN9_003258"
     CDS             complement(6557..7450)
                     /locus_tag="ACHUN9_003258"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0696582.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHUN9_003259"
     CDS             complement(7447..8100)
                     /locus_tag="ACHUN9_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0696583.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHUN9_003260"
     CDS             complement(8111..9370)
                     /locus_tag="ACHUN9_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0696584.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2494 g         2150 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgccccg atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZOYS010000157         9390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOYS010000157 DAZOYS010000000
VERSION     DAZOYS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.157"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(59..799)
                     /gene="cysQ"
                     /locus_tag="ACHUN9_003261"
     CDS             complement(59..799)
                     /gene="cysQ"
                     /locus_tag="ACHUN9_003261"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0696585.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            989..2932
                     /gene="cpdB"
                     /locus_tag="ACHUN9_003262"
     CDS             989..2932
                     /gene="cpdB"
                     /locus_tag="ACHUN9_003262"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0696586.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHYATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3050..3430)
                     /locus_tag="ACHUN9_003263"
     CDS             complement(3050..3430)
                     /locus_tag="ACHUN9_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0696587.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3519..4379
                     /gene="qorB"
                     /locus_tag="ACHUN9_003264"
     CDS             3519..4379
                     /gene="qorB"
                     /locus_tag="ACHUN9_003264"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0696588.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4487..5452
                     /locus_tag="ACHUN9_003265"
     CDS             4487..5452
                     /locus_tag="ACHUN9_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0696589.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5560..6222
                     /gene="ytfE"
                     /locus_tag="ACHUN9_003266"
     CDS             5560..6222
                     /gene="ytfE"
                     /locus_tag="ACHUN9_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0696590.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6267..7679)
                     /gene="cycA"
                     /locus_tag="ACHUN9_003267"
                     /gene_synonym="dagA"
     CDS             complement(6267..7679)
                     /gene="cycA"
                     /locus_tag="ACHUN9_003267"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0696591.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7911..8606)
                     /locus_tag="ACHUN9_003268"
     CDS             complement(7911..8606)
                     /locus_tag="ACHUN9_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFR0696592.1"
                     /translation="MGKEKKSRILTKPCVIEYEGRIVGYGSRELRVETISCWLARTII
                     QTKHYSRRFVNNSWLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKTIRLLYPSVEWVQSFADERCGRAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAINRGGKRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFRIQPYPK"
     gene            complement(8557..8742)
                     /locus_tag="ACHUN9_003269"
     CDS             complement(8557..8742)
                     /locus_tag="ACHUN9_003269"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0696593.1"
                     /translation="MDSVRCKNCNKLLFKGGFKHIEIKCPRCKRYIVISSAREHPTEL
                     YCGKREEITHSDKTLRY"
     gene            complement(8867..>9390)
                     /locus_tag="ACHUN9_003270"
     CDS             complement(8867..>9390)
                     /locus_tag="ACHUN9_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0696594.1"
                     /translation="AVDCDVFFDDRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIA
                     PFTVTPEMLEAAREARRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLA
                     KSVTAQTNMTWSDADNQQVQLTTQELEELATAMIQAIVERNDEIYRCQREMKEQLSLL
                     PTLDEVRAFRPGD"
BASE COUNT         2373 a         2466 c         2333 g         2218 t
ORIGIN      
        1 aggttgggag aaatcaggat ggcagaatca ggaaatacac tgtttctgcc atctgaattt
       61 agtaaataga cactctgaac cccggattca ggaacgactc gcgcggagtg taatccagcg
      121 gtttaccctg ccagtcgtga acgtgcgctc cggcagctgc agccacagca tgtccagcgg
      181 cggtgtccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt ccttccgcca
      241 ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca agctgttgca
      301 gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc ggcgggcgcg
      361 catcgcggac ctgaatctgc ttgcgcacac cgcactcttc tttccaggcc ttgccttctg
      421 ccgcgctgta cattacgttc attaccggcg catacaccac acctaaaatc ggtttgccat
      481 ggtcaatgag cgcaatgtta acggtgaatt cgccattacg tttaataaac tctttagtac
      541 catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc caaccgggag
      601 gatcttcttc agaaaggacc ggaatatccg gtgtcagcgt acgtaaaccg tccatgataa
      661 cggtgtgtgc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg ctgacgacgt
      721 ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc cgtgcaagct
      781 ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa cttattgttt
      841 tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat tttcaaagcg
      901 taaaattgtg gcattcttca ctgttctata agtaagacgt ttattctccc ttttctttcg
      961 tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc tcctggccac
     1021 gctgattgcc gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg aaaccactga
     1081 tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg aaaaattcgg
     1141 actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa acagcgtact
     1201 ggtcgataac ggcgatttga ttcaggggag tccgctggca gattacatgt cggcgaaagg
     1261 attaaaaaca ggtgatgttc atccggttta taaggcgctg aatacgctgg attatacggt
     1321 cggtacactc ggcaatcatg aatttaacta cggtctggat tacctgaaaa atgcgctggc
     1381 gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca aacagccaat
     1441 gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa aaaaacagac
     1501 gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg ataaagctaa
     1561 tttatccggg aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat acgtgcctga
     1621 aatgcgcgag aaaggtgccg atgtcgttgt cgttctggca cattccgggc tgtctgccga
     1681 tccgtataaa gtgatggcgg aaaactcagt ttattacctc agtgaaattc cgggcgttaa
     1741 cgccattatg tttggtcatg ctcacgccgt tttcccgggt aaagattttg ctgatatcga
     1801 aggggctgat atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc cgggcatgtg
     1861 gggcgatcat cttggggtgg tcgacttaca actcagtaat gacagcggta aatggcaggt
     1921 gacgcaggcg aaagcggaag ctcggccgat ttacgacatc gccaataaaa aatccctcgc
     1981 ggcggaagac agcaagctgg tagaaacact caaagccgat cactatgcca cacgccagtt
     2041 cgtcagcaag ccaatcggta aatctgccga caatatgtat agctatctgg cgctggtgca
     2101 ggacgatccg accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg aacattacat
     2161 tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgccgcac cgtttaaagt
     2221 tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc agttgacctt
     2281 ccgtaatgcc gccgatcttt atctctaccc caatacgctg attgtagtga aagccagcgg
     2341 taaagaggtg aaagagtggc tggagtgctc cgccggacag tttaaccaga ttgatcctaa
     2401 cagcacgaaa ccacagtcac tcatcaactg ggatggtttc cgcacttata actttgatgt
     2461 tattgatggt gtgaattatc agattgatgt tacccagccc gcccgttatg acggcgagtg
     2521 ccagatgatt aataccaatg cggaaaggat taagaacctg acctttaatg gcaagccgat
     2581 tgatccgaac gccatgttcc tcgttgccac caataactat cgcgcttacg gcggcaaatt
     2641 tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc gctcggtgct
     2701 ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcacccgg cggcagataa
     2761 caactggcgt ttagcaccga tagctgccga taagaaactg gatatccgtt tcgaaacttc
     2821 cccgtcaaat aaagccgcag cgtttattaa agagaaaggg cagtatccga tgaataaagt
     2881 cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt aaaacacttc
     2941 tttttcggcc tataaatcat caatcgcatc cggcatttat tggcggatgc gatgctggcg
     3001 catcttatcc gccctacaag tcatacaccg taggccgcgc atccggcagt tatgccgcac
     3061 gttcatcccg caccgccaac acctcgggca aattcaactc aatccagtcc gccagtgcag
     3121 caaccttttc gctcacctgc tcgcccagcg gcgtgaggct gtattcaaca tgcggtggca
     3181 ccaccggata cgcgatacgg ttaagaaaac catcctgttc taacgcctgt aacgactgcg
     3241 caagcatctt ttcgctcacc ccgccgattt tgcgccgcag gtcgctaaag cgatgagtac
     3301 cttcgcgtag cgccaccaga atcaacaccc cccaacggct ggtgacgtgt ttcaacacct
     3361 cgcgcgacgg gcactgttcc gcaaagagat taccctcttt cagttgttgc gacaggctaa
     3421 cctgactcat ttcatactta cctttttgta cgtacttact aaaagtaagt ttaggtgtta
     3481 gcgtatttaa acacaagaca aaacgatgga gacttcccat gatcgctatt actggtgcca
     3541 ctggccaact tggtcactat attattgaat ccttgatgaa aacggttcct gccagccaaa
     3601 tagtggctat cgttcgtaat ccggcaaaag cccaggctct agcagcacaa ggcattaccg
     3661 tgcgtcaggc tgactacggc gatgaagccg cactgacatc tgcacttcag ggagtggaaa
     3721 aactactgct gatctcttcc agcgaagtgg gtcaacgtgc cccgcagcat cgtaatgtta
     3781 ttaatgccgc aaaggcggct ggcgtgaaat ttatcgctta taccagcctg ctacatgcag
     3841 atacctcccc gctcggcctc gccgatgagc acattgagac ggagaaaatg ctggctgatt
     3901 ctggcatcgt ttacaccctg ctgcgcaacg gctggtacag cgaaaactac ctcgccagcg
     3961 ccccggcagc actggaacac ggcgtattta tcggtgcggc gggcgatggc aaaatcgcct
     4021 cagcaacgcg ggcagattat gcggcagctg cggcacgcgt gattagcgaa gccggtcacg
     4081 aaggcaaggt ttacgaactg gcgggcgata gtgcctggac gttgacacag ttagcggcag
     4141 agctgaccaa acagagcggc aaacaggtta cctatcaaaa tctgagcgaa gccgatttcg
     4201 ccgcggcact gaaaagcgtc ggactgcccg acggactggc ggatatgctg gcggattctg
     4261 acgttggcgc atcgaaaggt ggtctgtttg ataacagcaa aacgcttagc aaattgattg
     4321 gccgcccaac gacaacgtta gccgaaagcg taagccatct ttttaatgtt aataactagt
     4381 taattaaagt ggcatcctcc cgcatcctct ctgataatga cgggatgccg ggagcaatca
     4441 tgtctgcttc ctgaactttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt
     4501 gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt
     4561 accggaatac ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc
     4621 gctgcccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gcgactggct
     4681 taccgccttg atgctcacca tgatgggcaa ccttatctat tacttctgcc ttgccagtgc
     4741 cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat
     4801 tcccgttttt gccaatctgc tttatagcca gcgcgacggc aaactcgcat ggggaaaact
     4861 cgccccggca ctggtttgta ttggcatcgg cctggcgtgt gtgaatattg ctgagttaaa
     4921 ccacggactc cccgattttg actgggcacg ttatacctct ggcatcgtgc tggcgttagt
     4981 ttccgtggtc tgctgggcgt ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc
     5041 cgacaaacat ccgatgatgt gggcaacggc gcaggcgctg gtcacactgc cggtttctct
     5101 catcggctat ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc
     5161 ttttggcccc cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc
     5221 atgggttggc gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg
     5281 gccgctgatt gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca
     5341 aatgccgccg ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcattgc
     5401 ggtcagagca aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacacgctgg
     5461 cagtgagtta aataagcctc tgctacgtaa gggttatagc ttttgcctta aagatgcatt
     5521 taaaatacat cttatattat taagaatgag gtatcagcta tggcttatcg cgaccaacct
     5581 ttaggtgaac tggcgctctc tattcctcgc gcttcagctc tgtttcgtaa atatgatatg
     5641 gattactgct gtggcggtaa gcaaacgctg gcgcgcgcgg cggcacgtaa agaactggat
     5701 gttgaggtca ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg
     5761 cgtagcgccc cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac
     5821 cgcgagcaac tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac
     5881 aaaccgagcg tgccaaaagg gctgacaaaa tacctgacta tgctgcatga agagctatcc
     5941 agccacatga tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc
     6001 caggcaatgg gaccaatcag cgtgatggaa agcgagcacg atgaagcggg cgaactgctg
     6061 gaagtgatta aacacaccac caataacgtc acaccgccgc cggaagcctg caccacctgg
     6121 aaagcgatgt ataacggtat taatgaactg attgatgacc tgatggatca catcagcctg
     6181 gaaaacaatg tactgttccc acgcgcgctg gcgggtgagt aatctaaaag ctggatggca
     6241 ttgcgccatc cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca
     6301 ataaacaacc agcccagccc cagtgcgata aaccacagcg gggtgaccaa cagcgcctgg
     6361 cgagtgtcat cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc
     6421 cagcacatca gcttgccgag cggcatcttg tagatcgact tctcatgcag atgaggacgc
     6481 tgtttgcggt aaaccaggta cgagcaaagg ataattgtcc agacgaacat aaacagaatc
     6541 gcggaaacgg ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc
     6601 accacgccgc cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc
     6661 ttagaaagtt tagcgaacgc tttcggtgcc acaccttcct gcgccagacc aaacagcata
     6721 cggctggtag agaagacgcc gctgttagcg gaagacgccg cagaggtcag caccacaaag
     6781 ttgatcacgc tggcagccgc aggcaaacct accaacacaa acagttcaac aaacgggctc
     6841 ttctccggga ctaccgaact ccacggcgtc acggacataa tcacaatcag cgcgaagacg
     6901 tagaacataa tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga
     6961 tctttggttt ccgcagcggt tgtacctacc agctcaatcc ctacgaaagc gaaaaccgct
     7021 atctggaatc cggcaaagaa gccacttaaa cctttcggga accagccgcc atcattccac
     7081 aaatgcgcga atgacgcttc cacaccagtc ggtgactgaa agtgcatcgc caccataacc
     7141 aggccgacga caatcagcga cacaatggcg acgattttga tcatcgcaaa ccagaactcc
     7201 atctcaccga acattttcac ggtggcgaga ttgagcgtca gcagcagcac tatcaccgcc
     7261 agcgaggcga cccagtcgga gagatcgggg aaccagaact gagcataagc agtgatcgcc
     7321 accacgtctg ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat
     7381 cctgcccacg gcccgagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc
     7441 gaaagcagca attcccccat tgcccgcatc acgaaaaaga gcataaaacc gatgatcata
     7501 taaacgaaaa tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac
     7561 cccgtaccaa tggcaccgcc aatggcaata agctgaatat gtcggtttgt gagattgcgc
     7621 cgtagcgact gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg
     7681 tttttttctt cctgtacctg tctgtgttgt tcaggctcta cggcctttag tcagtctatg
     7741 atacgacgaa tctgtatcgg gactcatcga tattaggtaa caattaaagc gcgcaaaatt
     7801 attcaagata gatatggaat tctttcaaga taagccagaa gcgcgcatat ggaagttatt
     7861 caagataaaa cagcggcatt aaccgctgct tttttattca ttaagtggga tcacttcggg
     7921 taaggctgaa tcctgaatag cttagtattt agtcgctttc ttgcgcgctt gttcaggaac
     7981 ctgatgtaac gatattgatt aaatttgtgt accacggccc gctccttatt cgctcgtaaa
     8041 tattcgccgc gcttccctcc acgattaatc gcattcatcg ttatctcgtg ataccactca
     8101 ccgtccagtt cgtagaatgt gctttcatgg ctgccaataa aatcaaaatt tgacgcctga
     8161 tacacaacac ctgcacgccc gcaacgttcg tctgcaaagg actgaaccca ctccactgac
     8221 ggatacagta acctgatggt tttcagcgcg tagctgatgg cccgtgattc agagttgcgg
     8281 ggcatgtcgt cgtgtagcca catgcggttc agttccatat agccccggtt atccgtttca
     8341 agcacaacgc gacgccctga gttggggtta agggcatatc cccactgaag aacaccaacc
     8401 agatcacgtc cactgaatac gcccagatgc aaccaggagt tattcacaaa acggcgggaa
     8461 taatgctttg tctgaatgat tgtgcgggcc agccagcagg atatggtttc aacgcgcagc
     8521 tcccttgaac catagccgac aatccggcct tcatactcaa taacgcaggg ttttgtcaga
     8581 atgcgtgatt tcttctcttt tcccacaata cagctccgtg ggatgctccc gggcactcga
     8641 tatgacaatg taacgtttac agcgaggaca tttgatttct atatgcttaa aacctccttt
     8701 aaacagcaat ttattgcaat ttttacaacg aacagagtcc ataatttccc ccagtgtaat
     8761 aacaggatat agctgcccgg agggctgggg gtatttaatc acacttgatg caaaccgacg
     8821 atcgatatga tgctatcgat cgtcataaac tatacaaact gattatttaa tcgcctggtc
     8881 tgaacgctct cacctcgtcc agtgtgggca acagagaaag ttgttctttc atctcccgct
     8941 gacagcgata aatctcatcg ttgcgctcaa caatggcctg tatcatcgct gtggccagtt
     9001 cctcaagttc ctgcgtcgtc agctgcacct gctgattatc cgcatcactc cacgtcatat
     9061 ttgtttgtgc tgtgacagat tttgccagca tgactaccgg ggacaggcgg cccagtgagt
     9121 cggggccagc attccagata cgaccgttcc attcaaacgt gaacggcttc gcctcctgtt
     9181 ctgcgcgcca tgcttcaatt tcctgacgtc tggcctctct ggccgcttcc agcatttctg
     9241 gtgtcacagt gaatggggct atctcacccc atttgccgct ttgcagttcc tgccagattt
     9301 gctgacccgt cggtgcgaca tcatcagcgg tggctgtgta gggaactgcc tggtccctgt
     9361 cgtcaaaaaa aacgtcacag tctactgcgc
//
LOCUS       DAZOYS010000158         8990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOYS010000158 DAZOYS010000000
VERSION     DAZOYS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.158"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            308..2701
                     /gene="malP"
                     /locus_tag="ACHUN9_003271"
     CDS             308..2701
                     /gene="malP"
                     /locus_tag="ACHUN9_003271"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0696595.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2711..4795
                     /gene="malQ"
                     /locus_tag="ACHUN9_003272"
     CDS             2711..4795
                     /gene="malQ"
                     /locus_tag="ACHUN9_003272"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0696596.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4840..6156)
                     /gene="gntT"
                     /locus_tag="ACHUN9_003273"
     CDS             complement(4840..6156)
                     /gene="gntT"
                     /locus_tag="ACHUN9_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0696597.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6517..7092)
                     /gene="nfuA"
                     /locus_tag="ACHUN9_003274"
     CDS             complement(6517..7092)
                     /gene="nfuA"
                     /locus_tag="ACHUN9_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0696598.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7151..7834)
                     /gene="gntX"
                     /locus_tag="ACHUN9_003275"
     CDS             complement(7151..7834)
                     /gene="gntX"
                     /locus_tag="ACHUN9_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0696599.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7872..8642
                     /gene="bioH"
                     /locus_tag="ACHUN9_003276"
     CDS             7872..8642
                     /gene="bioH"
                     /locus_tag="ACHUN9_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0696600.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8671..>8990)
                     /locus_tag="ACHUN9_003277"
     CDS             complement(8671..>8990)
                     /locus_tag="ACHUN9_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0696601.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         2211 a         2240 c         2498 g         2041 t
ORIGIN      
        1 agcacattgg atattttact tgtgaaactg tttcactcct ttacttaatt tatagagtta
       61 ccttccgctt tttgaaaata cgcaacggcc attttttgca cttagataca gattttctgc
      121 gctgtattgc attgatttgc tgctaatcct gtggtttgca ctagctttaa gtggttgaga
      181 tcacatttcc ttgctcatcc ccgcaactcc tccctgccta atcccccgca ggatgaggaa
      241 ggtcaacatc gagcctggca aactagcgat aacgttgtgt tgaaaatcta agaaaagtgg
      301 aactcctatg tcacaaccta tttttaacga taagcaattt caggaagcgc tttcacgtca
      361 gtggcagcgt tatggcttaa attctgcggc tgaaatgact cctcgccagt ggtggctagc
      421 agtgagtgaa gcactggccg aaatgctgcg tgctcagcca ttcgccaagc cggtggcgaa
      481 tcagcgacat gttaactaca tctcaatgga gtttttgatt ggtcgcctga cgggcaacaa
      541 cctgttgaat ctcggctggt atcaggatgt acaggattcg ttgaaggctt atgacatcaa
      601 tctgacggac ctgctggaag aagagatcga cccggcgctg ggtaacggtg gtctgggacg
      661 tctggcggcg tgcttcctcg actcaatggc aactgtcggt cagtctgcga cgggttacgg
      721 tctgaactat caatatggtt tgttccgcca gtcttttgtc gatggcaaac aggttgaagc
      781 gccggatgac tggcatcgca gtaactaccc gtggttccgc cacaacgaag cactggatgt
      841 gcaggtaggg attggcggta aagtgacgaa agacggacgc tgggagccgg agtttaccat
      901 taccggtcaa gcgtgggatc tccccgttgt cggctatcgt aatggcgtgg cgcagccgct
      961 gcgtctgtgg caggcgacgc acgcgcatcc gtttgatctg actaaattta acgacggtga
     1021 tttcctgcgt gccgaacagc agggcatcaa tgcggaaaaa ctgaccaaag ttctctatcc
     1081 aaacgacaac catactgccg gtaaaaagct gcgcttgatg cagcagtact tccagtgtgc
     1141 ctgttcggta gcggatattt tgcgtcgcca tcatctggtg gggcgtaaac tgcacgaact
     1201 ggcggattac gaagttattc agctgaacga tacccaccca actatcgcga ttccagaact
     1261 gctgcgcgtg ctgatcgatg agcaccagat gagctgggat gacgcttggg ccattaccag
     1321 caaaactttc gcttacacca accataccct gatgccagaa gcgctggaac gctgggatgt
     1381 gaaactggtg aaaggcttac tgccgcgcca tatgcagatt attaacgaaa ttaatactcg
     1441 ctttaaaacg ctggtggaga aaacctggcc gggcgatgaa aaagtgtggg ccaaactggc
     1501 ggtggtgcac gataaacaag tgcatatggc gaacctgtgt gtggttggcg gtttcgcggt
     1561 gaacggtgtt gcggcgctgc actcggatct ggtggtgaaa gatctgttcc cggaatatca
     1621 ccagctatgg ccgaacaaat tccataacgt caccaacggt attaccccac gtcgctggat
     1681 caaacagtgc aaccctgcac tggcggctct gttggataaa tcactgcaaa aagagtgggc
     1741 taacgatctc gatcagctga tcaatctgga aaaattcgct gatgatgcga aattccgtca
     1801 gcaatatcgc gagatcaagc aggcgaataa agtccgtctg gcggagtttg tgaaagttcg
     1861 taccggtatt gagatcaatc cacaggcgat tttcgatatt cagatcaaac gtctgcatga
     1921 gtacaaacgc cagcacctga atctgctgca tattctggcg ttgtacaaag aaattcgtga
     1981 aaacccgcag gctgatcgcg taccgcgcgt cttcctcttc ggcgcgaaag cggcaccggg
     2041 ctactacctg gcgaagaata ttatctttgc gatcaacaaa gtggctgacg tgatcaacaa
     2101 cgatccactg gttggcgata agttgaaggt ggtgttcctg ccggattatt gcgtttcggc
     2161 ggcggaaaaa ctgatcccgg cggcggatat ctccgaacaa atttcgactg caggtaaaga
     2221 agcttccggt accggcaata tgaaactggc gctcaatggt gcgcttactg tcggtacgct
     2281 ggatggtgcg aacgttgaaa tcgccgagaa agtcggtgaa gaaaatatct ttatttttgg
     2341 tcataccgtg gaacaagtga aggcaattct ggccaaaggc tacgacccgg tgaaatggcg
     2401 gaagaaagat aaggtgctgg acgcagtatt gaaagagctg gaaagcggta aatacagcga
     2461 cggcgataag catgccttcg accagatgct gcacagtatc ggcaaacaag gcggcgatcc
     2521 gtatctggtg atggcggatt tcgcagccta tgttgaggca caaaagcagg tggatgtgct
     2581 gtaccgcgac caggaggcct ggactcgcgc ggcgatcctc aataccgccc gctgcggtat
     2641 gtttagctcg gatcgctcta ttcgcgatta tcaggctcgt atctggcagg caaaacgcta
     2701 aggaagctcg atggaaagca aacgtctgga taatgccgcg ctggcggcgg ggattagccc
     2761 caattacatc aatgcccacg gtaaaccgca gtcgattagc gccgaaacca aacggcgttt
     2821 gcttgacgcg atgcatcaac gtaccgccac gaaagtggcg gtaacgccag tcccgaatgt
     2881 catggtttat accagcggca aaaaaatgcc gatggtggtg gagggcagcg gcgaatatag
     2941 ctggctgctg accaccgaag aaggaacaca gtacaaaggc catgtaacgg ggggcaaagc
     3001 gttcaatcta ccgacgaagc tgccggaagg ttatcacacg ctgacactca cccaggacga
     3061 ccagcgcgcg cattgccggg tgattgtcgc cccgaaacgc tgttacgaac cgcaggcgtt
     3121 gctgaataaa caaaagctgt ggggtgcctg cgttcagctt tatacgctgc gatcggaaaa
     3181 aaactggggt attggggatt ttggcgatct caaagcgatg ctggtggatg tggcaaaacg
     3241 tggcgggtcg ttcatcggcc tgaacccgat tcatgcgctc tatccggcaa atccggagag
     3301 tgccagccca tacagcccgt cttctcgccg ttggctgaat gtgatttata tcgacgttaa
     3361 agccgtcgaa gatttccatc ttagcgaaga ggctcaggcc tggtggcagt tgccgaccac
     3421 gcaacagacg ctgcaacaag cgcgggatgc cgactgggtc gattactcca cggttaccgc
     3481 cctaaaaatg acagcattac gaatggcgtg gaaaggtttc gcgcaacgtg atgatgagca
     3541 gatggccgcg tttcgccagt ttgttgcaga tcaaggcgac agcctgttct ggcaggcagc
     3601 ctttgatgcg ctacatgccc agcaagtgaa agaggacgaa atgcgctggg gctggcctgc
     3661 atggccagag atgtatcaga acgtggattc accagaagtg cgtcagttct gcgaagaaca
     3721 tcgtaatgac gtcgattttt atctctggtt gcagtggctg gcttacagcc agtttgccgc
     3781 ctgctgggag ataagccagg gctatgaaat gccgattggc ttgtatcgtg atctggcggt
     3841 tggcgtagcg gaaggtgggg cggaaacctg gtgtgaccgt gaactatatt gcctgaaagc
     3901 gtcggttggc gcgccgccgg atatcctcgg cccgttgggg cagaactggg gattaccgcc
     3961 aatggacccg catatcatca ccgcgcgtgc ctatgaaccg tttatcgagc tgttgcgtgc
     4021 caatatgcaa aactgcggcg cattacgaat tgaccatgtg atgtcgatgc tgcgtttgtg
     4081 gtggataccg tatggcgaga cggcagatca gggcgcgtat gttcactatc cggtggatga
     4141 tctgctctcg attctggcac tcgaaagtaa acgtcatcgc tgtatggtga ttggtgaaga
     4201 tctcggtacc gtaccggtag agattgtcgg taagctgcgc agcagcggtg tgtactccta
     4261 caaagtgctc tatttcgaaa acgaccacga gaagacgttc cgtgcaccga aagcgtatcc
     4321 ggagcagtcg atggcggttg cggcgacaca tgacctgcct acgctacgcg gttactggga
     4381 gagcggggat ctaacgctgg gcaaaaccct ggggctgtat ccggatgaag tggtactgcg
     4441 cggtctgtat caggatcgcg aactggcgaa gcaagggctg ctggatgcac tgcataaata
     4501 tggttgtctg ccaaaacgtg ccgggcataa ggcatcgttg atgtcgatga cgccgacgct
     4561 gaaccgtggt ttgcagcgct acattgccga cagtaacagt gctctgttag gactacagcc
     4621 ggaagactgg ctggatatgg ccgaaccggt gaatattcct ggcaccagtt accagtataa
     4681 aaactggcga cgcaagcttt ccgcaacgct tgagtcgatg tttgccgatg atggcgtgaa
     4741 caagttgctg aaggatttgg acagacggcg cagagctgca gcgaagaaga agtagagtgc
     4801 ggttgatgcc ggatgcgatg catccggcag tcagtgttat caaatcacca tattcagcag
     4861 cagacagcct accagaccgc acaccgagat aatggtttcc agcatcgacc aggatttgat
     4921 ggtctcgccg atagtcaggt taaagtactc tttgaacagc cagaagcccg gatcgttcac
     4981 atgagagaaa atcacactac cggaacccac cgcaataacc atcagctctg ggctaacacc
     5041 cgtcgttgca atcagcggtg ccgcgatacc acccgcagtg attgccgcaa cggttgcgga
     5101 acccagcgcg atacgcagta cggcagcaat cgaccaggcc atcagcagcg gagaaatgtt
     5161 ggtttcgtgc atcatggaag caatgtattt gtccacgccg ctgtctacca gcacctgctt
     5221 gaacgcaccg ccaccaccga tgatcaacag catcatcgca atgattttga tggaagaaac
     5281 cagcgtgtcg ttaatctgat ccattgaacg accacggttc agaccaaagg tgaacatcgc
     5341 aatcagcacg gcaatcagcg ttgccattac cgggtcaccg aggaactccg ctaccggcag
     5401 gaaagcgtga cctttcggca ggatcatttc ggcaatcgca cgcatcgcca tcagcacaac
     5461 cggcaccaga gaagtccaga cgctgacgcc aaagctcggc atctcttctt cgctgaaggt
     5521 tttcgcgctg tagagacctt ccgggattgg cttatcgata cctttcaaca cgcgagcgta
     5581 aaccggaccg gcgagaatca cggtcgggat tgccagaata gtaccgtaca gcagggtttt
     5641 acccatatcg gcattgaaaa tggtggcaat cgcagtcggg cccggatgcg gtggcaggaa
     5701 gccgtgggtc acagacagtg cagccgccat tggtacacca acatacagca gtggaatatt
     5761 cgcggaagcc gcgatggtaa acaccagcgg cagcatcagc acgaagccca cttcatagaa
     5821 cagggcaaaa ccaacggtaa aaccggttaa caccaccgcc cactggatgt gctttttacc
     5881 aaatttggca atcagcgtgg tggcgatacg ttgtgcgcca ccgcagtctg ccagcatttt
     5941 gcccagcatt gcgccaaaac ccatgatcag ggcaaggcta ccgagcgtcc cgccgacacc
     6001 gactttgatg gagtcaataa ctttatccag cggcattcct tgcattaatc caacagcaag
     6061 cgccacgagg acgagagcga tgaagccgtt cattttgaag cggatcatca ggagcaacaa
     6121 caagattaca ccgatagcaa caatgactaa tggcatgatt tacctggcct ttcatttgtt
     6181 atgggtaacg tcaattttct gacgacaaac tctaattatc ccaatcggga acagagatat
     6241 tgcggcacca cgactgatac ccactaaaac taattattgt agtcagatgt caggagtatg
     6301 tttggtaccc atgtgaatga tacgggtaac atttggcgtt tgagaatcac cagagcgggg
     6361 taaatttaaa ttatgagagg ttggtcatat tatcgcgggg aaacgaaggg agggatttgc
     6421 caaagcaatg ctgtgccaac gtctggcaca tgttcaacgt aggcccgaaa tgacgcttta
     6481 gcgtcgcatc gggcaatcta caaaagaggg gataacttag tagtaggagt gttcgccgcg
     6541 ctggtgttcg gtgagatcgc gcacaccttt cagctccggg aattcgttca gcagctgctt
     6601 ctcgatccct tctttcagcg tcacatcgac catggaacaa ccgttacagc cgccgccaaa
     6661 ttgcagaatg gcgtaaccgt cttcggtgat ttccatcagc gaaacgcgac caccgtgacc
     6721 agcaagctgt gggttgatct gcgactgcag catatactcc acgcgctcca tcagcggtgc
     6781 atcgtctgcc actttacgca ttttggcgtt cggggctttc agcgttaact gagaacccaa
     6841 ctggtcggta acaaagtcga tctctgcatc ttccaggtat ggtgcgctta actcatcaac
     6901 atacgcggtc agcaggtcaa atttcagggc agtgtcggtg gcttccacgg catccggcgg
     6961 acaataagaa acaccacatt cagcgttagg cgtgccaggg ttaatcacaa atacgcggat
     7021 ttgtgtccct tcttcctgat ttgccagcag tttggcaaag tgcgcttgtg cagcatcgga
     7081 aatacggatc atagtaatgg cctaatagtt gactatttta gttggttata atacgcccat
     7141 catcgaggct ctacaaggtt cgacaaaggc accagacctg gacagtcgcc gcaccattgc
     7201 gtaaaagcaa ctgcgcaatc tctgcgacgg tacttccggt ggtaacgaca tcatccacaa
     7261 tcaccatatg gcgaccttgc acgggcaatt caagacgaaa ggcatttttc aggttgcgct
     7321 tgcgcagccg ggcactgaga aaatgctggg tcgcagtggc ccgtgtacgt gtgacggctt
     7381 cgctatccca ttggcagtgc aaccagcgtg ataacggctg acacagcaaa tcgctctgat
     7441 taaatccccg acgccagtga cgccgctgcc ataacggaac gctgatgatg cgatccggca
     7501 attgcaaccc ggtggtgcga cgagcgtgta agacttccaa tagtaacaga cgtgacaggg
     7561 cgctggcgat ttcactgcgc cgggaaaatt taagctggtg gatgagcaga cttaacggcg
     7621 gcgcatagtc ggcaaccgtg accagtcttt gccagggcgg cggtttttgc aggcagcgac
     7681 cgcagggaag atgggagtgt gtggcgggta atccacattg tgggcataac gttttatctg
     7741 tgcgggtggc gcgtgaacag accgaacaaa tcccccaatg acctaacgcc agtggcattc
     7801 ggcatagcca gcataatccc ggtactgtta gcatatgttc atccttgtaa gtcaaaagag
     7861 aacaatagcg gatgaataac atttggtggc agaccaaagg tcaggggaat gttcatcttg
     7921 tgctgctgca cggatgggga ctgaatgccg aagtgtggcg ttgcattgac gaggaactta
     7981 gctcgcattt tacgctgcac cttgttgatc tgcccggctt cgggcgcagc cagggatttg
     8041 gtgcgctgtc acttgctgat atggccgaag ccgtgctgca acaggcacct gataaagcca
     8101 tttggttagg ctggagtctg ggcgggctgg tggcaagcca gattgcgtta acccatcccg
     8161 agcgtgttca ggcgctggtc accgtggcgt cgtcaccttg ttttagtgct cgtgacgatt
     8221 ggccggggat aaaaccggac gtgctggcgg gatttcagca gcaactcagt gatgattttc
     8281 agcgtacagt ggagcggttc ctggcgttac aaaccatggg gactgaaacg gcgcgccagg
     8341 atgcgcgggc gttgaagaaa accgttctgg cgttaccgat gccggaggtt gacgtgctta
     8401 atggcgggct ggaaatcctg aaaacggttg atctccgtca gccgctgcaa aacgtgccca
     8461 tgccgttttt gcgattgtat ggctatctcg atggtctggt gccgcgcaaa gtggtgccga
     8521 tgctggataa actctggcct cgcagcaaat catatatctt cgccaaagcg gcccatgcgc
     8581 cgtttatttc gcatccggtc gagttttgtc acctgctggt ggcgttgaag cagagggtgt
     8641 aggcaacttt tgaaatggcg agacagaaat ttactggctc gccgcagcca actcttcttc
     8701 tgacaccccg gtaaagcgca tgatgtccgc aagagggact ccggattcaa gcattatttt
     8761 ggctatatgc agggctttgg attgttcccc ctcctgccgc aatctttccg caattgtcat
     8821 taaactctcc ttgtgtttcg gtgaacgttc ggcaacgccg tcgataaaat cgttaaaacg
     8881 taccgcgtcg ccggtttgca gtatgtaatt aaacagccct ttgatttgtc tgtcattagc
     8941 gtatccacta cttaataagc aggccatttg ctctaccagc cccatcaggt
//
LOCUS       DAZOYS010000159         8840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOYS010000159 DAZOYS010000000
VERSION     DAZOYS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.160"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUN9_003278"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUN9_003278"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0696602.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUN9_003279"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUN9_003279"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0696603.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUN9_003280"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUN9_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0696604.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUN9_003281"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUN9_003281"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0696605.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2168 a         2050 c         2260 g         2362 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
     8821 aaccttgaat tattattgct
//
LOCUS       DAZOYS010000160         8816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOYS010000160 DAZOYS010000000
VERSION     DAZOYS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.161"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            71..1606
                     /gene="lsrA"
                     /locus_tag="ACHUN9_003282"
                     /pseudo
     CDS             71..1606
                     /gene="lsrA"
                     /locus_tag="ACHUN9_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1600..1776
                     /locus_tag="ACHUN9_003283"
                     /pseudo
     CDS             1600..1776
                     /locus_tag="ACHUN9_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1784..1873)
                     /locus_tag="ACHUN9_003284"
                     /pseudo
     CDS             complement(1784..1873)
                     /locus_tag="ACHUN9_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHUN9_003285"
     CDS             complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHUN9_003285"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0696606.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3755..4705)
                     /locus_tag="ACHUN9_003286"
     CDS             complement(3755..4705)
                     /locus_tag="ACHUN9_003286"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0696607.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4844..5203)
                     /locus_tag="ACHUN9_003287"
     CDS             complement(4844..5203)
                     /locus_tag="ACHUN9_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR0696608.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHUN9_003288"
     CDS             complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHUN9_003288"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0696609.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHUN9_003289"
     CDS             complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHUN9_003289"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0696610.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7682..8563
                     /locus_tag="ACHUN9_003290"
     CDS             7682..8563
                     /locus_tag="ACHUN9_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0696611.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8640..>8816
                     /gene="yneK"
                     /locus_tag="ACHUN9_003291"
     CDS             8640..>8816
                     /gene="yneK"
                     /locus_tag="ACHUN9_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0696612.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKI"
BASE COUNT         2254 a         2322 c         2117 g         2123 t
ORIGIN      
        1 aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg caaaactgaa
       61 cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg cccgctcggt
      121 ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt tgcatcaagg
      181 ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat
      241 tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca actacgtcag
      301 attaacgcca gttcatgctc atcagctggg tatttatctc gttccccagg aaccgctgct
      361 tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa aacagctctc
      421 catgcagaaa atgaagaact tgctggcggc gctgggctgc tagtttgatc tgcatagtct
      481 ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg gcctgatgcg
      541 cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg cggaaaccga
      601 acgcttgttt cgtcgcttgc aagagctgct tgctactggc gtgggtattg tttttatctc
      661 gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc gcgacggaac
      721 catcgcctta agcggcaaaa ccagcgaact gtctaccgat gacattattc aggccatcac
      781 gccagtggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg agttacctgg
      841 caaccgccca caacatgccg ccggaacgtc ggtgctgaca ctagaaaatc tgaccggcga
      901 aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggtc tggccgggct
      961 ggtgggggcc ggacgcacag aactggccga gacgctctat ggtctgcgta ctttgcgtgg
     1021 cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag aacgtttact
     1081 gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc tcgatgcttc
     1141 gctggcctag aacgtctgcg cccttactca taaccttcgt ggattctggg cgaaaaccgc
     1201 gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat ttaaccaacc
     1261 ggaacaagct gcacggacat tatccggtgg caaccagcaa aaaatcctga ttgccaaatg
     1321 cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg tggatgtctc
     1381 ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg tggcagtgct
     1441 gcttatctcc tctgatctgg aagagatcga actgatggca gatcgtgtgt atgtgatgca
     1501 tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg agactattat
     1561 gcgcgttgcc ttcggcgata gtcagcgtca ggaggcgtca tgctgaagtt tattcagaac
     1621 aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgtattacc cggttttctc
     1681 gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc gcaaatcctg
     1741 atcctgctgg caatgggcgc gacgctggta atgcttatgc gcaaagtagt aagcgcgatg
     1801 atatcttgga taatactgag ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg
     1861 gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc cctattaaac gtgattacga
     1921 tcacattctc aagacgctac ttacaaatta cctacaagct tacaacatta acaggcgctg
     1981 catgtggcat ctgttaatga taaaggcaca taggatgttg taactaatat agtgaaataa
     2041 gaaacccggt caaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
     2101 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
     2161 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
     2221 ttcctgggta ccgataacat ggtcacgatg ctggctccag agttgctgat aacgttccag
     2281 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccac gatagaacgc
     2341 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccgtttg ccttctgccc
     2401 tgccagcagc tgcggcagga tgcgggtgcg gaacttggtc ataccgttga gcgcgataga
     2461 cagcagctga tgcttatacg ggttacggaa acgcccggtg actgcactgg cgaaagattc
     2521 cagttcatca cgaggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac
     2581 gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa
     2641 cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc gttctttata
     2701 cggtttaata tcgtcaacaa tcagcacgtt gagcggatat ttgtccaggc gcagctcagt
     2761 cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg tgtcgaggaa
     2821 accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg gataaccggt
     2881 aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa
     2941 tgcttctggc agtgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc
     3001 attgtagtcg atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa
     3061 atggctaaag cgttcgaaca gcagacgggt cagttttgcc ggatagctta tcgctggcgc
     3121 gtcatcgaat ttatcgcccg catggtagct gatccccgct tcggtggtgt tagagaaaac
     3181 aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac tgtagacgct
     3241 gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttccc ccttctcatt
     3301 caggccacgg ataatggtgg tatacagacc atcctgcgtg ctcagtgacg gcgggaatga
     3361 agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct cattcaggag
     3421 atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg
     3481 ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct
     3541 tctgattagt catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgcc
     3601 gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc
     3661 gcggatcatg gtttaatcga ggaaatacgc cttttcctgg atcataaagt ggtagaacac
     3721 attgcattca aatcgcgcgg aatgaataaa gatgtcagac aacttcctca ccgtaacgca
     3781 tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta tcggcttcga
     3841 ccatcaattt attaaaatca tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc
     3901 caatacttac cgtgagataa agcgtttttt gttgccaggt gaatggttgc agttcaacgc
     3961 ctttacggat tttttccgcc attagcagac catcgacagg attcaccgac ggcactgcaa
     4021 cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca atcttctgaa
     4081 tatgccgggc aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa
     4141 tgcttttgaa atagtcgata tcaagcagca tgaccgtcag aggcacgatc aacacgcccg
     4201 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
     4261 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
     4321 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
     4381 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
     4441 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
     4501 aatgcagtcg attaagccag acatagcggg caaacccccc cgccattacg ccattcagcg
     4561 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
     4621 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
     4681 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
     4741 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
     4801 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
     4861 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
     4921 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
     4981 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
     5041 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
     5101 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
     5161 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
     5221 taactgtttc gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc
     5281 cagttccgga ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc
     5341 accaacgcca gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta
     5401 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg
     5461 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gagccagctc
     5521 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc
     5581 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
     5641 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
     5701 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
     5761 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgtt ccatttccag
     5821 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
     5881 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
     5941 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
     6001 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
     6061 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
     6121 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
     6181 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
     6241 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
     6301 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
     6361 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
     6421 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacggggcc
     6481 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
     6541 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
     6601 cagggttttc tctacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
     6661 gagagtgttc tcttcgtcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
     6721 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
     6781 tacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
     6841 ggcatcgtta agcacaataa acggatccga accgcccagt tccagtacgc attttttcag
     6901 tgccgctcca gcctgtgcgc caatagccgc tccagcacga acacttccgg tcaccgtgac
     6961 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     7021 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
     7081 tcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     7141 agcgccacgc atcacctgcc ataacggaaa attccacggc ataatcgcca gaatcgtccc
     7201 caacggtcga tactcaataa ccgcctgctg attttccacc agtgtaggtt ccgccttcag
     7261 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
     7321 gcgcgcctgg ttgattggtt tgcccatttc gcgggtgatc atttgcgcca tttcttcgct
     7381 acgggcgcgc agagccttac cgatatcacg cattttttca gcacgataat ctatatttgt
     7441 ctcgcgccag tcgcgaaagc ctgctgccgc cagctcaagt gcgttttcga tatcgttagc
     7501 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
     7561 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
     7621 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
     7681 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
     7741 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
     7801 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
     7861 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
     7921 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
     7981 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
     8041 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
     8101 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
     8161 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
     8221 taacggcagt aacatttatg ccttccgcac caattgttcg tatcgtcgcc acttcgagag
     8281 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
     8341 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
     8401 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
     8461 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
     8521 actactggat gtgtctgact cggcaaaaca gagttaccaa tgaactattt tgatagttct
     8581 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
     8641 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
     8701 atattgcacc acaggtaaaa gaagttttaa aggactttat tgatgcgcta agtacaataa
     8761 tatgtgatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagata
//
LOCUS       DAZOYS010000161         8799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOYS010000161 DAZOYS010000000
VERSION     DAZOYS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.162"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..823
                     /gene="gadX"
                     /locus_tag="ACHUN9_003292"
     CDS             <1..823
                     /gene="gadX"
                     /locus_tag="ACHUN9_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0696613.1"
                     /translation="QSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVD
                     KCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1191..1919
                     /gene="gadW"
                     /locus_tag="ACHUN9_003293"
     CDS             1191..1919
                     /gene="gadW"
                     /locus_tag="ACHUN9_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0696614.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2282..5395)
                     /gene="mdtF"
                     /locus_tag="ACHUN9_003294"
     CDS             complement(2282..5395)
                     /gene="mdtF"
                     /locus_tag="ACHUN9_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0696615.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5420..6529)
                     /gene="mdtE"
                     /locus_tag="ACHUN9_003295"
     CDS             complement(5420..6529)
                     /gene="mdtE"
                     /locus_tag="ACHUN9_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0696616.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6637..6915
                     /locus_tag="ACHUN9_003296"
     CDS             6637..6915
                     /locus_tag="ACHUN9_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696617.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6916..7443)
                     /gene="gadE"
                     /locus_tag="ACHUN9_003297"
     CDS             complement(6916..7443)
                     /gene="gadE"
                     /locus_tag="ACHUN9_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0696618.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8242..>8799)
                     /gene="hdeD"
                     /locus_tag="ACHUN9_003298"
     CDS             complement(8242..>8799)
                     /gene="hdeD"
                     /locus_tag="ACHUN9_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0696619.1"
                     /translation="KATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDIL
                     STVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAI
                     AAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVS
                     TLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2352 a         2052 c         2061 g         2334 t
ORIGIN      
        1 gcaatcatta catgggaatt gtctaattgc gtacgcaaga cataaatata ttctcaccat
       61 ggttaatggt gaatatcgct attttaatgg cggtgacctg gtttttgcgg atgcaagcca
      121 aattcgagta gataagtgtg ttgaaaattt tgtattagta tcaagggata cgctttcatt
      181 atttctgccg atgctcaagg aggaggcatt aaatcttcat gcacataaaa aaatttcttc
      241 attactcgtt catcactgta gcagagatat tcctgttttt caggaagttg cgcaactatc
      301 gcagaataag aatcttcgct atgcagaaat gctacgtaaa agagcattaa tctttgcgtt
      361 gttatctgtt tttcttgagg atgagcactt tataccgctg cttttgaacg ttttgcaacc
      421 gaacatgcga acacgagttt gtacggttat caataataat atcgcccatg agtggacact
      481 agcccgaatc gccagcgagc tgttgatgag tccaagcctg ttaaagaaaa aattgcgcga
      541 agaagagaca tcatattcac agttgcttac tgagtgtaga atgcaacgtg ctttgcaact
      601 tattgttata catggctttt caattaagcg agtcgcagtg tcctgtggat atcacagcgt
      661 gtcgtatttc atttacgtct ttcgaaatta ttatgggatg acgcccacag agtatcagga
      721 gcgatcggcg cagggattgc cgaaccgtga ctcggcggca agtattgttg cgcaaggaaa
      781 tttttacggc actgaccgtt ctgcggaagg aataagatta tagaatttta ctcagacata
      841 aaaaaaaccc ggcatagggg accgggaaga ggatagtctg ccgtctccag actaataaac
      901 cgttataaca ctccctgttg gcacgggaaa ctttgtgctc tcagtaagtt aaatataact
      961 tttactggaa ataagatcag ccattttttt ataaacataa gctatacgct gtgcgaaaat
     1021 atattcgtgc tgcatttact tattatcaat taactgttat gcaaaactac tttgtggata
     1081 aattttggtc ctaccaaatc tggcagtttt ttcgctaaga aacagtctgg caacatttca
     1141 ttagtatact gaaattgaaa taatcgcagt atgaaatata agggataatc atgactcatg
     1201 tctgctcggt gatcctcatt cgtcgttcat tcgatattta tcatgaacag cataaaatat
     1261 cgctgcataa cgagagtatc gtgctgctgg agaaaaattt ggcagacgat tttgcgtttt
     1321 gttcaccgga tacgcgacga ctggatatcg atgagctgac agtttgccat tacttacaaa
     1381 atattcgtca gctaccacgc aatttagggt tacatagcaa agaccgtcta ttaattaacc
     1441 agtcaccccc catgccgctg gtgacggcga tttttgatag cttcaatgaa tccggggtaa
     1501 attcaccgat actgagcaat atgctctacc tttcctgttt atcgatgttt tctcataaga
     1561 aagaactgat ccccttactt ttcaatagca tcagcactgt ttcaggaaaa gttgaacgcc
     1621 ttattagctt tgatatcgcc aaacgttggt atctgcgcga tatcgcggaa agaatgtata
     1681 ccagcgagag tctcataaaa aaaaagttgc aggatgaaaa tacctgtttc agtaaaatat
     1741 tactcgcttc caggatgtcg atggccagac gattactcga gttacgtcaa attcctctgc
     1801 atactattgc agaaaaatgt ggctatagca gtacgtcgta ctttataaac acatttcgac
     1861 aatattatgg tgtaacgcca catcagtttg cgcaacattc gccaggtacc ttttcctgac
     1921 atattttgca tttgaatatt ggtcaggatc tcacacctgc ttcatgtgaa actcttccct
     1981 gatgatttct gccgggctac cggctagttc tctttcgcag tttgccaggc ggtataggta
     2041 ttttcaggag gaaaagactc ggcatgtttg ggattattaa gctgacaatt catacgatta
     2101 cgggaatgtg ggtcagtatt gtgctgttca aattgatgac gaatggctgg agtggtttct
     2161 actttcagtg ctgtgtacta agcctggtat tcctgacggt tagctggttg ttgagtggtg
     2221 aatggttagc aggaaagagt aaggctgaac cttcacgttc aaccttactc tcatttacac
     2281 gttacgcttt tttaaagcgg gcaaagagat gttccactac aacgaaaaag accggaacga
     2341 agtaaattgc cagcactgtt gcggcaaaca tcccgcccat cacgccggta cctaccgcgt
     2401 tttgcgcgcc agaaccggca ccatgactga taaccagcgg cagcacgccg agaataaagg
     2461 cgagagaggt catcaggatt gggcgtaaac gcatccgcgc cgcttcgatg attgcctcta
     2521 tcggcgtttt cccttctttc tgcatcatct caacggcaaa ttcgacaatc aggatggcgt
     2581 tcttggcgga aagcccgatg gtggtcagca aaccaacctg gaagtagacg tcattactta
     2641 agccgcgcag atcggtggcc agtaatgcgc caacgacgcc taacggaaca accaacatca
     2701 ccgagaacgg aattgaccag ctctcataga gtgcggcgag ggcgaggaac accacgacca
     2761 gtgaaatggc atacagtgca ggagcctggt ttgaggataa cgcttcctga tacgacagtc
     2821 cggtccatga gtagccgacg cctgccggaa gtttagcgac caggtctgcc ataaatttca
     2881 tagcgtcacc ggtacttttc ccggccgccg cttcacctaa aatctccatt gacgggatgc
     2941 cgttgtagcg ttccaatcgc ggtgaaccat aggtccattc ggtagacgag taggcagaaa
     3001 gcggtgccat cgtgccagag gcgttgcgta cataccattg gttgatgtta tccggcaaca
     3061 tacggaacgg cgtgcctgcc tggacataca cttttttcac ccgcccctgg ttgaggaagt
     3121 cgttcacgta gctgctgccg aaggcggtgg aaattgtctg gttgatatca gacagcgcca
     3181 cacccattgc ctcagctttc gcggcgttga cgttcacttt gaacatcggc gtatcttcca
     3241 ggccgttcgg gcgtaccccg gtgacctggt caggcgattg cgctgccagt gataacagct
     3301 cgtttcgtgc ctgggttagt ttttcgtgcc ccaggttacc gttgtccagc agttccatat
     3361 caaaacctga cgcggtaccc agttcagcca ccgcgggtaa gttgaacggg aagacgacgg
     3421 ctttattgat actgcttaac gcaatcattg cccgctgaat gatcgcggta accgagtttt
     3481 cctcaccgac acgttcagac cacggcttga gactgataaa cgccaggccg ttgttttgcc
     3541 cctggccgct gaagccaaag ccgccaacgg taaacaccga ctgaacatta tctttctctt
     3601 ttgtaagata ataatccgtc acctgttgca gcactttcgt ggtgttaacc atggtggcac
     3661 cggaaggtaa ctgcgcggtg gtcataaata ccccctgatc ctcttctggt aagaaagagg
     3721 tcggtgtacg caggaacagc accgccatcc cggcgcaaat cagcaggtag accaccatgt
     3781 agcgaccggt acaacgcaac agcgagcggg tgctatcggt atagtgctga gttgattttt
     3841 caaacagcgt gttgaagcgt gcgaacaggg cgttaggttt gtgaccgcct tccggcgcgg
     3901 ctttcagaat ggtggcgcac agggcagggg tcaggctcat tgccacaaat actgaaagca
     3961 gcatggagga gatcagcgtg atggagaact ggcggtagat ctccccggtt gcgccgctca
     4021 taaaggccat cggcataaac actgcggaaa gaacaacagc aataccgacc agcgcacgtt
     4081 ggatctgccc catcgattta tgcgtcgctt ccttcggtgg tagcttatct tccgcaatga
     4141 cacgctcgac gttctccacc accacgatgg cgtcatccac cagtaaccct atcgccagca
     4201 ccatcccgaa catagttaac gtgttgatgg tgaaaccgac cgccgacaag atcgcaaacg
     4261 tcccgagaat aaccaccggt acggcaatcg tcgggatgat tgtggcacgg aaattctgca
     4321 aaaacagata catgaccagg aagacgagga tgatagcctc aaccagtgtt ttaaaaactt
     4381 cctgaataga aatttcgata aacggcgtgg tgtcgtaagg ataaaccgtc ttcagacttg
     4441 cggggaaata ggctgataag cggttcagct cctctttgac tgcccgcgag gtatccaggg
     4501 cgtttgctcc ggtagccagt ttgatggcga tcccggcagc aggtttgccg ttatagcgcg
     4561 ccacggtgga ataatcttcc gctccaagtt cgacgcgggc gacatcacgc agcagcactt
     4621 gcgaaccatc ttgctgaact ttcaacagga ttttgccaaa ttcttccggc gtttgcagac
     4681 gcgtctgcac aatgatcgag gcgtttagtt gctggtctgc cgcctgtggc atgccaccca
     4741 gttgaccacc ggaaatctgg ttgttttgca ccttaatctg ggaaataaca tcggaaggta
     4801 ccagattgta tttattgagt ttttgcgggt ccagccagat acgcatggcg tactcggaac
     4861 caaagagctg tacgctacca acgcccgcgg tacggcttag cgggtctttg atattagacg
     4921 ctacatagtc cgcgatatcg tactggttga ggctgccgtt atcagaaata aacgccgcta
     4981 ccatcagaat attactgctc gacttatcaa cgctaatccc ctgctgctgc accgcttcag
     5041 gtaatgaagg catagcgagt tgcagtttat tttgcacctg aacctgtgcg atatcaggag
     5101 atgtcccagt ctcgaaggtc agagtgatag aggcattacc cgccgcatca ctggttgaag
     5161 acatgtacat caggccatca agcccattca tattttgctc aatcacctga gtgaccgagt
     5221 cttctaccgt ttgcgcatcg gcacctggat aggtagcgct gacggtaatg gtcggtggcg
     5281 caatctgcgg atactgcgca accggtaagt tcatgatcgc cagaccacct gcaagcatca
     5341 taataatggc aagtacccag gcaaaaaccg ggcgatcaat aaaatagtta gccatgaaag
     5401 tccccttaag cctgcaacgt tattgtttcg attcggtgct ggcgttttcc tggctggagg
     5461 aaattgctcg tgctttgata cccggacgaa tgcgttgcaa accggaaacg atcacccgat
     5521 cgccagcctg caagccagag gtgacaaccc actggtcgcc gatggctttg ctggcttcaa
     5581 tttcgcgtag ctgcacaaca tcgtctttat ccagaatgag cgccgttgct ttaccctggg
     5641 cgttgtgagt gacgccttcc tgcggcacca gtaatacatt ctggcggcta ccttcatcca
     5701 ctaatgccgt gacgtacatg ccaggcagca agtcaccatt tgggttgggg aaaatcgccc
     5761 gtaacgtcac ggagcccgtg gtttcatcca ccgtcgggtc ggagaatttc agtgtgccgg
     5821 tctggctgta gcgtttacca ttttccagat tgagctgtac tggcgtactg ccctgaacct
     5881 gtttgatttg cccactggcg acctcttctt tcatgcgcaa gaaatcctgc accgactgcg
     5941 tgagatcgac ataaatcggg tccagacgtt gtacggtaac cagcgaatct gcctgattag
     6001 cggtaacgag tgcgccgacg gtcaccgacg atttcccgct gacgcccgta atcggcgagg
     6061 tgacattcgc gtattgcaga ttgatcgtcg cctgttcaac agccgctttg gcgacggtga
     6121 cattggcttc tgcttcattc agctgggtgc gcgcggtgtc gtaatcctga cggctaacgt
     6181 agttggtctt cagcaacgat gcctggcggt taaaggtgat gcgggcattg ctggcggtag
     6241 agagcgcttt cgccagcgag cctttggcgg agtttagctc ggcctgtaaa ggtgcaggat
     6301 caatctgata cagcgaatcg ccctggttca ctttatcgcc ttcgataaag ttgcgtttaa
     6361 tgataatacc gcccacctgg ggacgtatct cggcaacttc ataaggaacg gttctaccgg
     6421 gcaattcgct caacacattg accgaaccgg gggagagtgt gacgacaccg acctcaggcg
     6481 tcatggcggc ggcgttttcc gccgatttgt catcgcaggc ggtgagcatc gcgccgcaga
     6541 ataacaacgg tattaacagc tttcttcttc tgttcatttt agtccctgaa aattcttgag
     6601 aaataacggt tctttaaatt gcgcgcgagg agagatatga gggatttaca gaccagcggc
     6661 atagtggggt tgtcagccag taaggtggga tacaggcaca gtgatcgaca tggtgaggtc
     6721 aacgacaaaa aaaatgttgc cgttctgcca acagttcctg ccagcattcg ggcaacgaag
     6781 ggttctactg gtggatacac atacctgggg aataaagtcg ttttagcctc tcctttagta
     6841 atcaccgggg gtaatgaaat cagcatctgc atccctcgtc atgccagcca tcaatttcag
     6901 ttgcttatgt cctgactaaa aataagatgt gatacccagg gtgacgatgt cgctcatacg
     6961 tcttagtttc agcttgtaca taatgttgaa tttctgaatc tttaatgttt gttgcgaaat
     7021 attgagcgtc gacgtgatat tgctttcatt tttatttttc agaatcagtt gcataatttt
     7081 gaactgatta tgtgaaaaac ataattgtga attcttatgg ggcaagtgtt taccataagt
     7141 aatctcaaga agatttttga tactttcttt gcggctaaca aaataaatag gcgctttagc
     7201 ttttagttta ctgatgtgat aaggtgccaa aacaataatt ctctcggcat ctaatttctc
     7261 cagaaattta atcgcttctt catcaaggat atgattgtga taggtatcaa tgataacctt
     7321 gaagggttta ttgcctactt ttttgatctc tgacagggaa ttgattttta tcatttcgtg
     7381 attatctttc aactgccaaa agccctgtaa aagaaaagaa tctttcgtca tgagaaaaat
     7441 cataacttgc tccttagccg ttatcgtttc gaacatgtca tccttttcat tatttacatc
     7501 cttgtccgaa tcgttgttca atatagtaaa cgcctataag aactttaccc ttcgtgaact
     7561 aatacgtcta ttaacactaa tcaatacttc agatttatcc atcacgcact ccatatattg
     7621 atcctaagta agattcctgg ttgttatcag cttgtaaata gtaaacaaaa agttaacgtt
     7681 tgcggattac tgcccaagaa taagttatca ataaccagtt tgtgatctct gaagaatatt
     7741 actaaagtta aaatgtaatc cgatttaaat atcgagtctc cttgtttcga cttaagctgg
     7801 caattggatt gccagctttc ttttaacgca ttgaaattac ctgagtaaat aacgcattca
     7861 tgtcttcagg tttttatata gcgtctggag acacggatat ttatgcaatg taatcaacgc
     7921 cagttgcaaa aaaatgaacc cggattactt atttaaccgc ccatagcatc aacgcttacg
     7981 gaactctttg ttcactttcc cacagcactt ttaaaacatt aaacctgact acggaaaata
     8041 tcagccatga taatgtttga aatggctaat ttgccatgga gtgaaaaaaa ttagatgaaa
     8101 ttcagtaggt tgaaataatc actagcaggt aattatttca atgatagtgc gcaattgatc
     8161 tacaacactc cgtagcggag agagtattaa tcggatcata gtcatatcaa gtgactatga
     8221 tccgggtgac aaccgggata attattgctg cttaacgaac aaactggcga agctgaacag
     8281 gctggcggcg ctaaatatca gttcaattcc caccagtgtg gaaaccagcg ttacagacac
     8341 catcggcgtt gcaccaagga atatccaggc aatgacgata tccagcacac caataacgag
     8401 ctgtagccag ctgcctttca ttgaacgctg acgataccaa ctcatcaggc gaataacccc
     8461 tgcaacacag aacaaaccgg caataaatgc cgcaatggca aaaatgccca gctccggtgc
     8521 gcggatgaag aaatagccga tcaataaata ggcgactgcg acgaggaaac cggataatac
     8581 cggccagaaa ttatgactgc ggttgctgaa taacccgaca ataagcgcaa tgcccgagca
     8641 gattaataat gcacccacta ctgtgcttaa aatatcgcca gagacgaacg ggaaactgat
     8701 acacagcaac ccgacgataa acagtagcac ggcaataaac tggattgctc tgcgatgttt
     8761 tttaagcatc tccagatcaa acttcaaaat tgttgcctt
//
LOCUS       DAZOYS010000162         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOYS010000162 DAZOYS010000000
VERSION     DAZOYS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.163"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUN9_003299"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUN9_003299"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0696620.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUN9_003300"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUN9_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR0696621.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUN9_003301"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUN9_003301"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR0696622.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUN9_003302"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUN9_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR0696623.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUN9_003303"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUN9_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR0696624.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUN9_003304"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUN9_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR0696625.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUN9_003305"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUN9_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR0696626.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUN9_003306"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUN9_003306"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0696627.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHUN9_003307"
     CDS             6614..7768
                     /locus_tag="ACHUN9_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR0696628.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUN9_003308"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUN9_003308"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR0696629.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2315 g         2051 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZOYS010000163         8668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOYS010000163 DAZOYS010000000
VERSION     DAZOYS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.164"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(301..507)
                     /locus_tag="ACHUN9_003309"
     CDS             complement(301..507)
                     /locus_tag="ACHUN9_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0696630.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="ACHUN9_003310"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="ACHUN9_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0696631.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="ACHUN9_003311"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="ACHUN9_003311"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0696632.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="ACHUN9_003312"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="ACHUN9_003312"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0696633.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="ACHUN9_003313"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="ACHUN9_003313"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0696634.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="ACHUN9_003314"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="ACHUN9_003314"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0696635.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="ACHUN9_003315"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="ACHUN9_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0696636.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1820 a         2248 c         2491 g         2109 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgattg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatt
//
LOCUS       DAZOYS010000164         8658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOYS010000164 DAZOYS010000000
VERSION     DAZOYS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.165"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(237..1901)
                     /gene="glnS"
                     /locus_tag="ACHUN9_003316"
     CDS             complement(237..1901)
                     /gene="glnS"
                     /locus_tag="ACHUN9_003316"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0696637.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2160..4106)
                     /gene="nagE"
                     /locus_tag="ACHUN9_003317"
     CDS             complement(2160..4106)
                     /gene="nagE"
                     /locus_tag="ACHUN9_003317"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0696638.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4439..5239
                     /gene="nagB"
                     /locus_tag="ACHUN9_003318"
     CDS             4439..5239
                     /gene="nagB"
                     /locus_tag="ACHUN9_003318"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0696639.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5299..6447
                     /gene="nagA"
                     /locus_tag="ACHUN9_003319"
     CDS             5299..6447
                     /gene="nagA"
                     /locus_tag="ACHUN9_003319"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0696640.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6456..7676
                     /gene="nagC"
                     /locus_tag="ACHUN9_003320"
                     /gene_synonym="nagR"
     CDS             6456..7676
                     /gene="nagC"
                     /locus_tag="ACHUN9_003320"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0696641.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7724..8476
                     /gene="nagD"
                     /locus_tag="ACHUN9_003321"
     CDS             7724..8476
                     /gene="nagD"
                     /locus_tag="ACHUN9_003321"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0696642.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8527..8658)
                     /locus_tag="ACHUN9_003322"
                     /pseudo
     CDS             complement(8527..8658)
                     /locus_tag="ACHUN9_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2119 a         2280 c         2208 g         2051 t
ORIGIN      
        1 gaagaaaagc agaaaaaacg ccgctgaacg cggcgttttg gattgtttgc atatttcttg
       61 ccggatgcgg cgtgaacgcc ttaaccggcc tacaaaagca cgcaaactca atatattgca
      121 gagatcatgt aggcctgata agcgtagtgc atcaggcaat ttagcgtttg aaactgtttg
      181 tctgttcctt gccggatgcg acgtaaacgc cccatccggc atagcgaaac ttaaaattac
      241 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc
      301 gccgtagaat ggcggctatc gaggcagaag taaccttcac gctcaaactg gaatgcttta
      361 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
      421 gggttaatca ccgacaggaa atcatccgca gcaccagggt tcggcacgct gaacagacgg
      481 tcgtacagac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
      541 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
      601 atggtggtga tattgccttc ggcatctttc tccacgcgtt ctgccttaat gacgtaagca
      661 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
      721 cggaaatcgg cgcgatcgat ccaaatctca ccgctaaacg gcacctggcg gctacccatt
      781 tccggtttgt tcggatggtt cggcatggta accatttcgc cttcgccctg atagttttcg
      841 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gggcgttttc gttgagatct
      901 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg
      961 atgcgtttgc agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa
     1021 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
     1081 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
     1141 gggtgaacag gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg
     1201 aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg
     1261 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
     1321 atgcggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca
     1381 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg
     1441 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg
     1501 cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc
     1561 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
     1621 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct
     1681 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
     1741 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
     1801 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
     1861 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
     1921 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttagc
     1981 gaaaatcgaa aatctcactg agattatggg gatgattgat gaaaaaaaaa gcggcagaga
     2041 agatctccac cgcttttgcc agtcccgcgt cattgttgga tgcgcgtctt atccaaccag
     2101 gactgagctt ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca taaagcagat
     2161 tactttttga tttcatacag cggtgtttga cccgccacaa cattgccctg agctttaatg
     2221 atcaagccac tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgaacgggcg
     2281 ttagcgttca ggtaatccag atccatttcc agaatcggtt gccctgcgct cacctgcgcg
     2341 ccctcttcca ccagacgttt aaagccttta ccttccagcg ctacggtgtc gatacccatg
     2401 tggacgacaa tctccgcgcc tttttcggtt tctaggcaga acgcgtggtt ggtgttgaag
     2461 attttcacga ttgtccctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc
     2521 acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag aaacctgatc cagtgccacg
     2581 acatcaccgg taatcggcga taccagctcc gcgatagata ccgcgtttgg tacagcctgc
     2641 ggttttgcta caggagcggc agttgccgga gttgcttcag ctgacgcagc tgctaccggg
     2701 ccacgggcaa cgactttctt catcgcatcg ccaatggatt ctgctttcgc gccaacaatc
     2761 acctgaatgg tctgtttgtt cagttttact acaccagaag cacccagacg tttacacatc
     2821 gcatcgttga cgcgagctga gtcagccacg gtaaggcgca gacgggtaat acaggcgtca
     2881 atcgctttca ggttgtcagt accgccaacc gcagcaatat agttggttgc cagttgagtc
     2941 agaccttctt cagtattgct gttagcctct tcagttacga tctcgtcttc tttatcttca
     3001 cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata
     3061 gcgaagaaga caacacccat caccagcagc atccagacgt tctggctggc ggccggcagg
     3121 ttatacatca acgcgtagtc gatagccccc gcagagaaag agaagccagc atggataccc
     3181 agcagcgttg ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc
     3241 ggagcaagga acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta
     3301 acagcaacag aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag
     3361 tacatcgcca gcgccgcacc cggcagaccg aacatcatga tcgggaagaa gccggacatg
     3421 aacatccccg cggtgccgtc accagcgtag aaacggttga tgtcgccgtg gaaaaccgta
     3481 cccgccgcgt tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc
     3541 agaccggttg ggatcagcag acggttgatg aaaccaaaga taccggaacc cagcgcgcct
     3601 gcagaaacga tccactcgcc gcctgcatgg atagcgtgct gtactggcgg ccagacgtaa
     3661 ccaaaaatgg ccgccagcac caggcagaag aagccggtgg caatcggcac aaagcgtttg
     3721 ccgccgaaga agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg
     3781 ccaccaacca gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg
     3841 gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac ccgccagcgc cgccgcacct
     3901 gcgctgtctt tcgaccagct ggatgccaca ccgatggcga agattaatgc gaggttatca
     3961 aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat
     4021 cgcagcaaca gtgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt
     4081 cgctggaaaa aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt
     4141 ttaaagccgt atttttattt tgctgcaaat tgtactgccg atgttctgta atcagattgt
     4201 tagatcatct gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgcgtgcc
     4261 ttttgtgagt tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaagcaga
     4321 aaacttattt tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccagg
     4381 ttacgcttaa agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat
     4441 gagactgatc cccctaacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt
     4501 caatcgtatc aatgcgttca aaccgactgc cgatcgtccg tttgttctgg gcctgccgac
     4561 tggcggcacg ccgatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt
     4621 cagctttaag cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca
     4681 tccggaaagc tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc
     4741 agaaaacatc aaccttctca acggcaacgc cccggatatc gacgccgaat gccgccagta
     4801 tgaagaaaaa atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga
     4861 cggtcatatt gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac
     4921 cctgactcat gacactcgcg tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt
     4981 gccaaaatat gccctgactg tcggtgttgg tacattgctg gatgccgaag aagtgatgat
     5041 tctggtgctg ggtagccaga aagcactggc actgcaggct gcagttgaag gttgcgtgaa
     5101 ccacatgtgg accatcagct gtctgcaact gcatccaaaa gcgatcatgg tgtgcgatga
     5161 accttccacc atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga
     5221 aaatatcaaa ggtctgtaat tgttatccct gccctggctc cttgctcagg gcaatatttt
     5281 ttaaaatcgg ggggcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa
     5341 tttcttgatg accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta
     5401 gcggaactgc cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt
     5461 tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg
     5521 gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac
     5581 tatctgccga cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg
     5641 cgcgagtacc tggcaagaca tccgaatcag gcgttaggtc tgcatctgga aggtccgtgg
     5701 ctgaatctgg taaaaaaagg cacccataat ccgaattttg tgcgtaagcc tgatgccgcg
     5761 ctggtcgatt tcctgtgtga aaacgccgac gtcattacca aagtgaccct ggcaccggaa
     5821 atggttcctg cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgccggt
     5881 cactccaacg cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat tacctttgcc
     5941 acccatctgt acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg
     6001 atcctcgacg aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac
     6061 gccaacattc gtaacgctaa acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc
     6121 accgcgccag caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac
     6181 cgtaacggac tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt
     6241 gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg
     6301 gcgacgctct atccggcgcg tgcgattggc gttgagaaac gtctcggcac actcgccgca
     6361 ggtaaagtag ccaacctgac ctcattcaca cctgatttta aaatcaccaa gaccatcgtt
     6421 aacggtaacg aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga
     6481 taggtaatgt tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc
     6541 agtacgggcc aatctcgcgg attcagattg ccgagcaaag ccagcttgcc cccgccagcg
     6601 taaccaaaat tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg
     6661 cctccaccgg gggccgccgc gctatctcca tcgtcaccga aacccgcaat ttccacgcaa
     6721 tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct gtttgatctc agcagcaaag
     6781 tgctggcaga agaacattac ccgctgccgg aacgtaccca gcaaacgctg gaacatgccc
     6841 tgttgaatgc cattgctcag tttattgata gctaccagcg caaactgcgc gagctgatcg
     6901 cgatttcggt gatcctgcca gggcttgttg acccggacag cggcaaaatt cattacatgc
     6961 cgcatattca ggtagaaaac tgggggctgg tagaagctct ggaagaacgt tttaaagtga
     7021 cctgtttcgt tggtcacgat atccgtagtc tggcgctggc ggagcactac ttcggtgcaa
     7081 gtcaggattg cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta
     7141 tctctaacgg gcgcattttt attggccgca acggtaacgt cggtgaaatt ggccatattc
     7201 aggtcgaacc gctgggtgaa cgctgccact gcggcaactt tggctgcctg gaaactatcg
     7261 ccgccaacgc tgccattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc
     7321 gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata
     7381 gtctggcgtc ggaagtaatt gagtatgtcg gtcgtcatct gggtaaaacc atcgccattg
     7441 ctatcaactt atttaatccg caaaaaattg ttattgccgg tgaaatcacc gaagccgata
     7501 aagtgctgct ccctgctatt gaaagctgca ttaataccca ggcgctgaag gcgtttcgta
     7561 ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc ggcaatcggc gcttttgcgc
     7621 tggtaaaacg cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg
     7681 cttttatagt ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt
     7741 aatttgcgat atcgacggcg tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga
     7801 atttttgcac gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc
     7861 gcagaccggg caagatctgg cgaaccgctt tgccactgcc ggtgtcgatg tgccggatag
     7921 cgtgttttat acctctgcga tggcgaccgc cgatttcctg cgtcgccagg aaggcaagaa
     7981 agcatatgtg gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat
     8041 taccgatgtg aaccctgatt tcgtgattgt tggcgaaacg cgttcctaca actgggacat
     8101 gatgcataaa gcggcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga
     8161 cacccacggg cgcggttttt atcccgcttg tggcgcgttg tgtgcaggga ttgagaaaat
     8221 ctccgggcgc aaaccgttct atgttggtaa gcccagcccg tggatcatcc gcgcagcatt
     8281 aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc ggcgataact tgcgcaccga
     8341 cattctggcg ggcttccagg caggtctgga aacgattctg gtgctttctg gtgtttcatc
     8401 gctcgacgat atcgacagta tgccgttccg cccaagctgg atttacccgt cagtcgctga
     8461 aatcgacgtt atctgaatac cacccacgtc tgcggacgtg gttggctctg atgacaaacg
     8521 ccaaactgcc tgatgcgcta cgcttatcag gcctacgcag ctcctgcaat atattgaatt
     8581 ttcatgcttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caaatcgcac
     8641 tttatcagca atttggcc
//
LOCUS       DAZOYS010000165         8648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOYS010000165 DAZOYS010000000
VERSION     DAZOYS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.166"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(271..534)
                     /gene="rpsT"
                     /locus_tag="ACHUN9_003323"
     CDS             complement(271..534)
                     /gene="rpsT"
                     /locus_tag="ACHUN9_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0696643.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            637..855
                     /locus_tag="ACHUN9_003324"
     CDS             637..855
                     /locus_tag="ACHUN9_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0696644.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            863..1804
                     /gene="ribF"
                     /locus_tag="ACHUN9_003325"
     CDS             863..1804
                     /gene="ribF"
                     /locus_tag="ACHUN9_003325"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0696645.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1847..4663
                     /gene="ileS"
                     /locus_tag="ACHUN9_003326"
     CDS             1847..4663
                     /gene="ileS"
                     /locus_tag="ACHUN9_003326"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0696646.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4663..5157
                     /gene="lspA"
                     /locus_tag="ACHUN9_003327"
     CDS             4663..5157
                     /gene="lspA"
                     /locus_tag="ACHUN9_003327"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0696647.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5282..5731
                     /gene="fkpB"
                     /locus_tag="ACHUN9_003328"
     CDS             5282..5731
                     /gene="fkpB"
                     /locus_tag="ACHUN9_003328"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0696648.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5733..6683
                     /gene="ispH"
                     /locus_tag="ACHUN9_003329"
                     /gene_synonym="lytB"
     CDS             5733..6683
                     /gene="ispH"
                     /locus_tag="ACHUN9_003329"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0696649.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6749..7663
                     /gene="rihC"
                     /locus_tag="ACHUN9_003330"
     CDS             6749..7663
                     /gene="rihC"
                     /locus_tag="ACHUN9_003330"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0696650.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7830..>8648
                     /gene="dapB"
                     /locus_tag="ACHUN9_003331"
     CDS             7830..>8648
                     /gene="dapB"
                     /locus_tag="ACHUN9_003331"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0696651.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1969 a         2161 c         2460 g         2058 t
ORIGIN      
        1 taatacctca atgtgtatca caatatccac attctttgta ggggagtctg gagattgagt
       61 agatattctt gttcagaatg tatcagccga tggttctacg attcttaagc cacgaagagt
      121 tcagaaagtg taacggcatg tctgcaaaag ccagcatctt ttgactatct ggcaaccggt
      181 agtgtgttct ctcacgcatc ataaaagcag caggcataaa aaaaaacccg cttgcgcggg
      241 ctttttcaca aagcttcagc aaattggcga ttaagccagt ttgttgatct gtgcagtcag
      301 gttagcctta tgacgtgcag ctttgttttt gtggatcaga cctttagcag cctgacggtc
      361 cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc cagcttcgat
      421 agctgcgtat actttcttga tgaaagtacg catcatagag cgacggcttg cgttgtgctt
      481 acgagccttt tcagactgaa tggcgcgctt cttagctgat ttgatattag ccaaggtcca
      541 actcccaaat gtgttctata tggacaattc aaaggccgag gaatatgccc ttttagcctt
      601 cttttgtcaa tggatttgtg caaataagcg ccgttaatgt gccggcactc gttacgtagt
      661 gatggcgcag gattctacca gcttgcgggg tgtgaataca gcttttccgc gataaaaatt
      721 gcagcaggcg gtcactttct tcccgtgatt tgcgccatgg caatgaaaag ccacttcttt
      781 ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat actcggacga
      841 ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc tcagccaggc
      901 cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc gcggtcatcg
      961 cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg tgatggtgat
     1021 gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg caagactgac
     1081 ccggctgcgg gaaaaactgc gttaccttgc agagtgtggc gttgattacg tgctgtgcgt
     1141 gcgtttcgac aggcgtttcg cggcgttaac cgcgcaaaat ttcatcagcg atcttctggt
     1201 gaagcatttg cgcgtaaaat ttcttgccgt aggggatgat ttccgctttg gcgctggtcg
     1261 tgaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg atatcactag
     1321 tacgcaaact ttttgcgaag gtggcgtgcg tattagcagc accgctgtgc gtcaggcgct
     1381 ggcggatgac aatctggctc tggcagaaag tttactgggg cacccgtttg ccatctccgg
     1441 gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg cgaatgtacc
     1501 gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc tgggcctcgg
     1561 tgaaaagccg ttacccggcg tggcaaacat cggaacacgc ccaacggttg ccggtattcg
     1621 ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc gccatataca
     1681 agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcgtcgctgg acgaactgaa
     1741 agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa caaaaccggc
     1801 ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga gtgactataa
     1861 atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg ccaagcgcga
     1921 accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcatcc gtgcggctaa
     1981 aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg gcagcattca
     2041 tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca aagggctttc
     2101 cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga tcgagctgaa
     2161 agtagagcaa gaatacggta agccgggtga gaaattcacc gccgccgagt tccgcgccaa
     2221 gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta tccgtctggg
     2281 cgtgctgggc gactggtcgc acccgtacct gaccatggac ttcaaaactg aagccaacat
     2341 catccgcgcg ctgggcaaaa tcatcggcaa cggtcacctg cacaaaggcg cgaagccagt
     2401 tcactggtgc gttgactgcc gttctgcgct ggcggaagcg gaagttgagt attacgacaa
     2461 aacttctccg tccatcgacg ttgcttttca ggcggtcgat caggatgcac tgaaagcaaa
     2521 atttgccgta agcaacgtta acggcccaat ctcgctggtg atctggacca ctacgccgtg
     2581 gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg cgctggtgca
     2641 gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa tgcagcgtat
     2701 cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcggagcttg agttgctgcg
     2761 ctttacccat ccgtttatgg gcttcgacgt tccggcaatc ctcggcgatc acgttaccct
     2821 agatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg actatgtgat
     2881 cggtcagaaa tacggcttgg aaaccgctaa cccggttggc ccggacggca cttatctgcc
     2941 gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca tcgtcgttgc
     3001 gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca gctatccgtg
     3061 ctgctggcgt cacaaaacgc cgatcatctt ccgcgcgacg ccgcagtggt tcgtcagcat
     3121 ggatcagaaa ggtctgcgtg cgcagtcact gaaagagatc aaaggcgtgc agtggatccc
     3181 ggactggggc caggcgcgta tcgagtcgat ggttgctaac cgtcctgact ggtgtatctc
     3241 ccgtcagcgc acctggggcg taccgatgtc actgttcgtg cacaaagaca cggaagagct
     3301 gcatccgcgt actctcgaac taatggaaga agtggcaaaa cgcgttgaag ttgacggcat
     3361 ccaggcgtgg tgggatcttg atgcgaaaga gatcctcggc gatgaagctg atcagtacgt
     3421 gaaagtgccg gacacattgg atgtatggtt tgactccgga tctacccact cttctgttgt
     3481 tgacgtgcgt ccggaatttg ccggtcacgc agcggacatg tatctggaag gttctgacca
     3541 gcaccgtggt tggttcatgt cttccctaat gatctccacc gcgatgaaag gcaaagcgcc
     3601 gtatcgtcag gtactgaccc acggctttac cgtggatggt cagggtcgca agatgtctaa
     3661 atccatcggc aataccgttt cgccgcagga tgtgatgaac aaactgggcg cggatattct
     3721 gcgtctgtgg gtggcatcaa ccgactacac cggtgaaatg gccgtttctg acgagatcct
     3781 gaaacgtgct gccgatagct atcgtcgtat ccgtaacacc gcgcgcttcc tgctggcaaa
     3841 cctgaacggt tttgatccag caaaagatat ggtgaaaccg gaagagatgg tggtactgga
     3901 tcgctgggcc gtaggttgtg cgaaagcggc acaggaagac atcctcaagg cgtacgaagc
     3961 atacgatttc cacgaagtgg tacagcgtct gatgcgcttc tgctccgttg agatgggttc
     4021 cttctacctc gacatcatca aagaccgtca gtacaccgcc aaagcggaca gtgtggcgcg
     4081 tcgtagctgc cagactgcgc tgtatcacat cgcagaagcg ctggtgcgct ggatggcacc
     4141 aatcctctcc ttcaccgctg atgaagtgtg gggctacctg ccgggcgaac gtgaaaaata
     4201 cgtcttcacc ggtgagtggt acgaaggcct gtttggcctg gcagacagtg aagcgatgaa
     4261 cgatgcgttc tgggacgagc tgttgaaagt gcgtggcgaa gtgaacaaag tcattgagca
     4321 agcgcgtgcc gacaagaaag tgggtggctc gctggaagcg gcagtaacct tgtatgcaga
     4381 accggaactg tcggcgaaac tgaccgcgct gggcgatgaa ttacgatttg tcctgttgac
     4441 ctccggcgct accgttgcag actataacga cgcacctgct gatgctcagc agagcgaagt
     4501 actcaaaggg ctgaaagtcg cgttgagtaa agccgaaggt gagaagtgcc cacgctgctg
     4561 gcactacacc caggatgtcg gcaaggtggc ggaacacgca gaaatctgcg gccgctgtgt
     4621 cagcaacgtc gccggtgacg gtgaaaaacg taagtttgcc tgatgagtca atcgatctgt
     4681 tcaacagggc tacgctggct gtggctggtg gtagtcgtgc tgattatcga tctgggcagc
     4741 aaatacctga tcctccagaa ctttgctctg ggggatacgg tcccgctgtt cccgtcgctt
     4801 aatctgcatt atgcgcgtaa ctatggcgcg gcgtttagtt tccttgccga tagcggcggc
     4861 tggcagcgtt ggttctttgc cggtattgcg attggtatta gcgtgatcct ggcagtgatg
     4921 atgtatcgct cgaaggccac gcagaagcta aacaatatcg cttacgcgct gattattggc
     4981 ggcgcgctgg gcaacctgtt cgaccgcctg tggcacggct tcgttgtcga tatgatcgac
     5041 ttctacgtcg gcgactggca cttcgccacc ttcaaccttg ccgatactgc catctgtgtc
     5101 ggtgcggcac tgattgtgct ggaaggtttt ttgccttcta gagcgaaaaa acaataataa
     5161 accctgccgg atgcgatgct gacgcatctt atccggccta cagattgctg cgaaatcgta
     5221 ggccggataa ggcgtttacg ccgcatccgg caaaaatcct caaatataag agcaaacctg
     5281 catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc taaaactcga
     5341 cgatggcacc accgccgagt ctacccgcaa caacggtaaa ccggcgctgt tccgcctggg
     5401 tgatgcttct ctttctgaag ggctggagca acacctgttg gggctgaaag tgggcgataa
     5461 aaccaccttc tcgttggagc cagatgcggc gtttggcgtg ccgtcaccgg acctgattca
     5521 gtacttctcc cgccgtgaat ttatggatgc aggcgagcca gaaattggcg caatcatgct
     5581 ttttaccgca atggatggca gtgagatgcc tggcgtgatc cgcgaaatta acggcgactc
     5641 cattaccgtt gatttcaacc atccgctggc cgggcagacc gttcattttg atattgaagt
     5701 gctggaaatc gatccggcat tggaggcgta acatgcagat cctgttggcc aacccgcgtg
     5761 gtttttgtgc cggggtagac cgcgctatca gcattgttga aaacgcgctg gccatttacg
     5821 gcgcaccgat atatgtccgt cacgaagtgg tacataaccg ctatgtggtc gatagcttgc
     5881 gtgagcgtgg ggctatcttt attgagcaga ttagcgaagt accggacggc gcgatcctga
     5941 ttttctccgc acacggtgtt tctcaggcgg tacgtaacga agcaaaaagc cgcgatttga
     6001 cggtgtttga tgccacctgt ccgctggtga ccaaagtgca tatggaagtc gcccgcgcca
     6061 gccgtcgtgg cgaagagtcg attctcatcg gccacgccgg gcatccggaa gtggaaggta
     6121 caatgggcca gtacagtaac ccggaagggg gaatgtatct ggtcgaatcg ccggacgatg
     6181 tgtggaaact gacggtcaaa aacgaagaga agctctcctt tatgacccag accacgctgt
     6241 cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg aaaattgtcg
     6301 gtccgcgcaa agatgacatc tgctacgcca cgactaaccg tcaggaagcg gtacgcgccc
     6361 tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa aaactcctcc aactccaacc
     6421 gtctggcgga gctggcccag cgtatgggca aacgcgcgtt tttgattgac gatgcgaaag
     6481 atatccagga agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg ggcgcatcgg
     6541 ctccggatat tctggtgcaa aatgtggtgg cacgtttgca gcagctgggc ggtggtgaag
     6601 ccattccgct ggaaggccgt gaagaaaaca ttgttttcga agtgccgaaa gagctgcgtg
     6661 tcgatattcg tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa atgccggtct
     6721 tgttaccggc attttttatg gagaaaacat gcgtttacct atcttcctcg atactgaccc
     6781 cggcattgac gatgccgtcg ccattgccgc cgcgattttt gcccccgaac tcgacctgca
     6841 actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa accacccgca acgccctgca
     6901 actgctgcat ttctggaatg cggagattcc gctcgcccag ggagccgctg tgccgctggt
     6961 acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg ggaatggctg gctacgactt
     7021 tgttgagcac aaccgaaagc caatcgggat accagcgttt ctggcgattc gtgatgccct
     7081 gatgcgtgca ccagagcctg ttaccctggt ggccattggc ccgttaacca atattgcgct
     7141 gttactttca caatgcccgg aatgtaaacc gtatattcgt cgtctggtga tcatgggcgg
     7201 ttcttctggt cgcggcaact gtacaccaaa tgccgagttt aatatcgctg ccgatccaga
     7261 agcggctgcc tgtgtcttcc gtagcggaat cgaaatcgtc atgtgcggtc tggatgtcac
     7321 caatcaggca atattaaccc ctgactatct cgctactttg ccggagttga atcgcaccgg
     7381 aaaaatgctt catgcactgt ttagccacta ccgtagcggc agtatgcaaa gcggtctgcg
     7441 aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg gacttattca ccctcaagcc
     7501 gtgttttgtc gcggtagaaa ctcaaggcga atttacctca ggcacgacgg tggttgatat
     7561 cgacggttgc ctgggcaaac cagccaatgt aaaggtggca ttggatctgg atgtgaaagg
     7621 cttccagcag tgggtggctg aggtgctggc tctggcgtcg taacctgtca catgttatcg
     7681 gcatgcagtc attcatcgac tcatgccttt cactgatatc cctccctgtt tatcattaat
     7741 ttctaattat cagcgttttt ggctggcggc gtagcgatgc gctggttact ctgaaaacgg
     7801 tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc aaacatccgc gttgccatcg
     7861 cgggagccgg ggggcgtatg ggccgccagt tgattcaggc ggcgctggca ttagaaggcg
     7921 tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt actgggcagc gacgccggtg
     7981 agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag cagcctcgat gcggtaaaag
     8041 atgattttga tgtgtttatc gattttaccc gtccggaagg tacgctgaac catctcgctt
     8101 tttgtcgcca gcatggcaaa gggatggtga tcggcactac ggggtttgac gaagccggta
     8161 aacaggcgat tcgtgacgcc gccgccgata ttgcgattgt ctttgccgcc aattttagcg
     8221 ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc caaagtgatg ggtgactaca
     8281 ccgatatcga aattattgaa gcacatcata gacataaagt tgatgcgccg tcaggcaccg
     8341 cactggcaat gggagaggcg atcgcccacg cccttgataa agatctgaaa gattgcgcgg
     8401 tctacagtcg tgaaggccac accggtgaac gtgtgcctgg caccattggt tttgccaccg
     8461 tgcgtgcagg tgacatcgtt ggtgaacata ccgcgatgtt tgccgatatt ggcgagcgtc
     8521 tggagatcac ccataaggcg tccagccgta tgacatttgc taacggcgcg gtaagatcgg
     8581 ctttgtggtt gagtggtaag gaaaatggtc tttttgatat gcgagatgta cttaatctca
     8641 ataatttg
//
LOCUS       DAZOYS010000166         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOYS010000166 DAZOYS010000000
VERSION     DAZOYS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.167"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUN9_003332"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUN9_003332"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0696652.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUN9_003333"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUN9_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0696653.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUN9_003334"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUN9_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0696654.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUN9_003335"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUN9_003335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0696655.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUN9_003336"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUN9_003336"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0696656.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUN9_003337"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUN9_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0696657.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUN9_003338"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUN9_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0696658.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZOYS010000167         8584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZOYS010000167 DAZOYS010000000
VERSION     DAZOYS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.168"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            375..1352
                     /gene="glaH"
                     /locus_tag="ACHUN9_003339"
     CDS             375..1352
                     /gene="glaH"
                     /locus_tag="ACHUN9_003339"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0696659.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1372..2640
                     /gene="lhgO"
                     /locus_tag="ACHUN9_003340"
     CDS             1372..2640
                     /gene="lhgO"
                     /locus_tag="ACHUN9_003340"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0696660.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2663..4111
                     /gene="gabD"
                     /locus_tag="ACHUN9_003341"
     CDS             2663..4111
                     /gene="gabD"
                     /locus_tag="ACHUN9_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0696661.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4125..5405
                     /gene="gabT"
                     /locus_tag="ACHUN9_003342"
     CDS             4125..5405
                     /gene="gabT"
                     /locus_tag="ACHUN9_003342"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0696662.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5716..7116
                     /gene="gabP"
                     /locus_tag="ACHUN9_003343"
     CDS             5716..7116
                     /gene="gabP"
                     /locus_tag="ACHUN9_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0696663.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7137..7799
                     /gene="csiR"
                     /locus_tag="ACHUN9_003344"
     CDS             7137..7799
                     /gene="csiR"
                     /locus_tag="ACHUN9_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0696664.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7800..8249)
                     /gene="kbp"
                     /locus_tag="ACHUN9_003345"
     CDS             complement(7800..8249)
                     /gene="kbp"
                     /locus_tag="ACHUN9_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0696665.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8333..8491)
                     /locus_tag="ACHUN9_003346"
     CDS             complement(8333..8491)
                     /locus_tag="ACHUN9_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0696666.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1946 a         2233 c         2385 g         2020 t
ORIGIN      
        1 gggaaaacag gagaaccgcc gaactatctc tatttataat ttaatgttat atctgccccg
       61 ataaaacggg gcagataata tgtttagttt actaacggtc attttgcagt gaagccattt
      121 actgtttttt atcgaccaga taatctgttc tctaatgtta actcccccta acctgttgct
      181 ttagttattc atttcctgtc tcactttgcc ttaatactct acgttaaatg ttactaattt
      241 gttgcttttg atcacaataa gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg
      301 tagatatttt tagattatgg ctacgaaatg agcatcgcca tgtcacccta catctcataa
      361 gaggatcgct tctgatgaat gcactgaccg ccgtacaaaa taacgctgtc gattcaggcc
      421 aggactatag cggattcact ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca
      481 ccttcaccga acagacgacc aaacagtttc tcgagcaggt tgccgaatgg cccgtacagg
      541 cgctggagta caaatcgttt ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg
      601 cgaatcagct gcaaccattg ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt
      661 tgatcaatgc ggtgggtatc gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa
      721 cggcggtagc gcatctgatt ggtcgctcaa atttcgatgc gatgagcggt cagtattacg
      781 cgcgtttcgt ggtgaaaaat gtcgataact cagacagcta tctgcgtcag ccgcaccgcg
      841 tgatggaact gcacaacgac ggcacgtatg tcgaagagat caccgattac gtgctgatga
      901 tgaaaatcga tgagcaaaac atgcagggtg gaaattcgtt gctgctgcat ctcgatgact
      961 gggaacatct ggaccactat ttccgccatc cactggcgcg tcgcccgatg cgctttgccg
     1021 cgccgccgag caaaaacgtc agcaaagatg ttttccatcc agtgttcgac gttgatcaac
     1081 agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat tttgaagaag
     1141 gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag caaaggcatt ctttctgtac
     1201 ccgttcccgt tggcaaattc ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc
     1261 gctttactcc gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt
     1321 acgccactca ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat
     1381 tttgtgatta ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtt
     1441 tatccggatg cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg
     1501 ggccacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggcg
     1561 cagttttgcc tggcggggaa ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc
     1621 tatgacaact gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc
     1681 gccttatggg aacgcacagc ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa
     1741 ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc gtccagcggc
     1801 attgtcagct accgcgaagt cacagcggca atggcaaaaa tcttccaggc cagaggcggc
     1861 gagattattt ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg cgtggtgata
     1921 cgcacccgtc agggcggtga atatgaagca tcgacgctga ttagctgttc cgggctgatg
     1981 gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttatcatctg cccgttccgt
     2041 ggtgagtatt tccgccttgc gccggagcat aaccagattg ttaaccacct gatttacccc
     2101 attcccgacc ccgcgatgcc atttttgggc gtgcatctca cccgcatgat cgacggcagc
     2161 gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac
     2221 ttctcattta gtgacacgct ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa
     2281 aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctacctg
     2341 cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc
     2401 ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga ttgacgattt tctgtttgtc
     2461 accaccccac gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt
     2521 cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc
     2581 ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa
     2641 cctttgaaaa caggacgtaa caatgaaact taacgacagt aacttattcc gccagcaggc
     2701 gttgattaac ggggaatggc tggacgccaa caatggcggg atcatcgacg tcaccaatcc
     2761 ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc
     2821 tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc
     2881 caacattctg cgcaactggt tcaatttgat gatggagcat caggacgatt tagcgcgcct
     2941 gatgaccctc gaacagggca aaccactggc cgaagcgaaa ggcgaaatca gctacgctgc
     3001 ctcttttatt gagtggtttg ccgaagaagg caaacgtatt tatggcgata ccattcccgg
     3061 tcatcaggcc gataagcgcc tgattgttat caagcagccg attggcgtta ccgcagctat
     3121 cacgccgtgg aacttcccgg cggcgatgat tacccgtaaa gccggtccgg cgctggcggc
     3181 aggctgcacc atggtgctga agcccgccag tcagacaccg ttctctgcgc tggcgctggc
     3241 ggagctggcg attcgcgcgg gcattccggc tggcgtgttt aacgtagtca ccggttcggc
     3301 aggcgcggtc ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg
     3361 ttcgaccgaa attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc
     3421 gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc
     3481 cgtggaaggc gcgctggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa
     3541 ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt
     3601 gagcaaactg cacatcggcg acgggctgga taaaggcgtc accatcgggc cgctgatcga
     3661 tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaga aaggtgcgcg
     3721 tgtggtttgc ggcggtaaag cggatgaacg cggcggcaac ttcttccagc cgaccattct
     3781 ggtggacgtg ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc
     3841 gctgttccgc tttaaagatg aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg
     3901 ccttgccgcc tatttctacg cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct
     3961 ggagtacggc atcgtcggca tcaataccgg cattatttcc aatgaagtgg ccccgttcgg
     4021 cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt
     4081 agaaatcaaa tatatgtgca tcggtcttta actggagaat gcgaatgaac agcaataaag
     4141 agttaatgca gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt cacccgattt
     4201 tcgctgaccg cgcggaaaac tgccgggtgt gggacgttga aggccgtgag tatcttgatt
     4261 tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca tccgaaggtg gtggccgcag
     4321 tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt
     4381 atctggagct gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc
     4441 tgctggttac gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca
     4501 ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg cattacacgc
     4561 tggcgctgac cggcaaggtg aatccgtact ctgcgggcat ggggctgatg ccgggtcatg
     4621 tttatcgcgc gctttatcct tgcccgctgc acggcataag cgaggatgac gctatcgcca
     4681 gcatccaccg gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc atcgtgattg
     4741 agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac
     4801 gcgctctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg
     4861 ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt ggcgccggat cttaccacct
     4921 ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga
     4981 tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg
     5041 tggctgcgct ggaagtgttg aaggtgttcg agcatgaaaa tctgctgcaa aaagccaacg
     5101 atctggggca gaagctaaaa gacggattgc tggcgatagc cgaaaaacac ccggagatcg
     5161 gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat ggcgatcaca
     5221 gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat aagggcctga
     5281 ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg ctcaccattg
     5341 aagacgctca gatccgtcag ggtctggaga tcatcagcca gtgttttgct gaggcgaagc
     5401 agtagctatg tccgtttgta agagttaacc gttgctaagg gaatgataac gatgcagtaa
     5461 aaaataataa cgatgaggcg aacgacaaag ttaaaaacga ctgcggacag tcccctcgcc
     5521 cctctggaga gagggttagg gtgaggggta aaacacgcgg ctaaaccctc ccctggccct
     5581 ctccctaagg gagagggaga aaaccctccc gctttcgttt ttaagatttt gttgaccgtc
     5641 tcaattaaac gcgtgacccg gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt
     5701 tcgagaggat tcaggatggg gcaatcatcg caaccacatg agttaggcgg cgggctgaaa
     5761 tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt
     5821 tccagcgtcg ccatcgccga agcgggcccg gcggtattac tggcctatct gttcgccggg
     5881 ctactggtgg ttatgattat gcggatgttg gcggaaatgg cggttgccac gcccgatacc
     5941 ggttcgtttt ccacctatgc cgataaagcc attggtcgct gggcgggcta taccatcggc
     6001 tggttgtact ggtggttttg ggtactggtt atcccgctgg aagccaatat cgccgctatg
     6061 atcctgcact cgtgggttcc aggcattccc atctggttat tttccctcgt cattaccctt
     6121 gccttaactg gcagtaattt attaagcgtt aaaaactacg gcgaatttga gttctggctg
     6181 gcgctgtgca aagtcatcgc tatcctggcc tttattttcc ttggtgcagt cgcaattagc
     6241 ggtttttacc cttatgccga agtgagcggg atctcaagat tgtgggatag cggcggcttt
     6301 atgcccaacg gtttcggtgc ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg
     6361 ggcgcagaaa ttgtcaccat tgccgccgcg gaatccgaca cgccggaaaa acatattgtc
     6421 cgcgccacca actcggttat ctggcgtatt tctatcttct atttgtgctc tatttttgtc
     6481 gtcgtggcat taataccgtg gaatatgccg gggctgaaag ccgttggatc ttatcgctcg
     6541 gttctggaat tgctcaatat tccccatgcg aaattaatca tggactgtgt gatattactt
     6601 tccgtaacca gttgcctgaa ctcggccctg tataccgcct caaggatgct ctactcctta
     6661 agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac
     6721 gtggcggtgt tactctccac cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg
     6781 cctgcgaagg tatttaaatt tctgatcgac agctccggcg ctatcgccct gctggtttat
     6841 ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc tgcgagcgga aggaagcgaa
     6901 attcgcttgc gaatgtggct ttatccctgg ctcacctggc tggtcatcgg ctttattacc
     6961 tttgtgttgg tagtgatgct attccgtccg gcgcaacagt tagaagtgat ctccaccggc
     7021 ttattagcga tagggattat ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta
     7081 ttgtggcaaa aaacacccgt tcataatacg cgctgataat gatcaggagt cacaccatga
     7141 ccattacgtc tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc
     7201 aaccggatga aaaattacgg atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc
     7261 cgttgcggga agcactttct caactggtgg cggaacggct ggtcacggtg gtgaatcaaa
     7321 aagggtatcg ggtggcgtct atgtcagagc aggagctgct cgatattttc gatgcccgcg
     7381 ccaatatgga agcgatgtta gtgagtctgg cgattgcccg cggtggcgat gagtgggagg
     7441 cagacgttct cgcaaaagcg catctgctca gtaagctgga agcctgtgac gccagcgaga
     7501 aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct
     7561 gtggttctca ctatttgctg caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc
     7621 gatttatctg gctgcggcga acggtgcttt cggtagaaat gctggaggat aaacacgatc
     7681 agcaccagac cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa
     7741 tgcgccagca tttactgacg ccaattccca ttatccagca ggcgatggct ggcaattaac
     7801 tactcttccg gaatacgcaa cacttgcccc ggataaattt tatccgggct ttttagcatc
     7861 ggtttattcg cttcgaagat tttattgtac agattagcgt taccgtagac ctgtttggaa
     7921 atggcactca gagtgtcgcc agacttaacg gtataaaact ggctggcagt ggctggtgtc
     7981 gccgttttca cctgatcatc aacactggca ataccggaaa tattccccac cgcaacaagg
     8041 attttctcct tcgcctcctg actcaggccg tcaccagtga ccgtcgcttt gccgtcggca
     8101 atttgaatgt tcactttatc agcatcaggt ataccggttt tgctcagatg ctcctgcacc
     8161 ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag tttttctccg
     8221 gcatctttca caaaattgaa cagacccata gctacctcat tcgttaacgg aaacactcag
     8281 aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc
     8341 gggtttgcac ccagaacgcg tgaatcaaac cggggatata cccaagcagt gtcagcagaa
     8401 tattaataat gaacgcccaa ccgaaccctt taccgagcag cacgccgagc ggcggtagaa
     8461 tgatggtgat gacgattctc cagaaaccca tatgtactcc ctataagaaa attactcatt
     8521 gtttaaaaag agattttatc tcttaagcgt agtaatttta gcggagcctg ccagtttcag
     8581 ctta
//
LOCUS       DAZOYS010000168         8430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOYS010000168 DAZOYS010000000
VERSION     DAZOYS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.169"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUN9_003347"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUN9_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0696667.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUN9_003348"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUN9_003348"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0696668.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUN9_003349"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUN9_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUN9_003350"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUN9_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0696669.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUN9_003351"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUN9_003351"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0696670.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUN9_003352"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUN9_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0696671.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUN9_003353"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUN9_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0696672.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUN9_003354"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUN9_003354"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0696673.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2118 a         2115 c         2079 g         2118 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga
//
LOCUS       DAZOYS010000169         8243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOYS010000169 DAZOYS010000000
VERSION     DAZOYS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.170"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHUN9_003355"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHUN9_003355"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0696674.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHUN9_003356"
     CDS             complement(643..882)
                     /locus_tag="ACHUN9_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0696675.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHUN9_003357"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHUN9_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0696676.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHUN9_003358"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHUN9_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0696677.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHUN9_003359"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHUN9_003359"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHUN9_003360"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHUN9_003360"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHUN9_003361"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHUN9_003361"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHUN9_003362"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHUN9_003362"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0696678.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHUN9_003363"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHUN9_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0696679.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHUN9_003364"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHUN9_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0696680.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /gene="yecU"
                     /locus_tag="ACHUN9_003365"
     CDS             7047..7223
                     /gene="yecU"
                     /locus_tag="ACHUN9_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0696681.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /gene="yecF"
                     /locus_tag="ACHUN9_003366"
     CDS             7210..7434
                     /gene="yecF"
                     /locus_tag="ACHUN9_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0696682.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHUN9_003367"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHUN9_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0696683.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2054 a         2097 c         1921 g         2171 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctg
//
LOCUS       DAZOYS010000170         8217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOYS010000170 DAZOYS010000000
VERSION     DAZOYS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.171"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUN9_003368"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUN9_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0696684.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUN9_003369"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUN9_003369"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0696685.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUN9_003370"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUN9_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0696686.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="ACHUN9_003371"
     CDS             2031..2549
                     /locus_tag="ACHUN9_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0696687.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUN9_003372"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUN9_003372"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0696688.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUN9_003373"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUN9_003373"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0696689.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUN9_003374"
                     /gene_synonym="ydfH"
     CDS             5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUN9_003374"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0696690.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUN9_003375"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUN9_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0696691.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="ACHUN9_003376"
     CDS             complement(6713..8173)
                     /locus_tag="ACHUN9_003376"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0696692.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2172 a         2055 c         1872 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca agctatatgt tttgtta
//
LOCUS       DAZOYS010000171         8181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOYS010000171 DAZOYS010000000
VERSION     DAZOYS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.172"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..2846
                     /gene="yfaL"
                     /locus_tag="ACHUN9_003377"
     CDS             <1..2846
                     /gene="yfaL"
                     /locus_tag="ACHUN9_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0696693.1"
                     /translation="DGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQTF
                     VHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTGD
                     IVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVAV
                     DAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVA
                     DIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNAS
                     LFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGD
                     TLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGI
                     KVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNPN
                     PTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQ
                     TLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQG
                     DSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDGT
                     DHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDI
                     QTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTGN
                     ISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2974..3696)
                     /gene="ubiG"
                     /locus_tag="ACHUN9_003378"
     CDS             complement(2974..3696)
                     /gene="ubiG"
                     /locus_tag="ACHUN9_003378"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0696694.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3843..6470
                     /gene="gyrA"
                     /locus_tag="ACHUN9_003379"
     CDS             3843..6470
                     /gene="gyrA"
                     /locus_tag="ACHUN9_003379"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0696695.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6678..>8181
                     /locus_tag="ACHUN9_003380"
     CDS             6678..>8181
                     /locus_tag="ACHUN9_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0696696.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2320 g         1851 t
ORIGIN      
        1 ccgacggaaa aacgctggtt attggcaata cagagaatga cggagctgtt gactctattg
       61 ctggtaccgg gttgatcacc aaaacaggtt ccggcgatct ggtacttaat gcagataaca
      121 atgactttac tggtgagatg cagattgaaa acggtgaagt taccctgggc cgcagcaact
      181 ccctgatgaa tgtcggcgat acgcattgcc aggacgatcc gcaagactgc tacggtctga
      241 cgatagggag tattgataag taccagaatc aggcagagct aaacgttggc tccacccaac
      301 aaacctttgt gcactcattg acgggctttc agaatggcac tttaaatatc gatgctggtg
      361 gcaatgttac tgttaatcaa ggcagttttg ctggcaccat cgaaggtgct ggtcagctca
      421 ccattgcgca aaacggcagc tacgtgctgt cgggggcgca gtcgatggcg ctaaccggcg
      481 atatagtcgt tgatgatggt gcggtgcttt cgctggaagg cgacgcggca gatctcgccg
      541 ctctccagga cgatccgcag tcgatcgtgt taaacggcgg tgtgctcgat ctctctgatt
      601 tctccacctg gcagagcggc acatcataca atgatggcct tgaagtcagt ggcagcagcg
      661 gaacggttat cggcagtcag gatgtggtag atcttgcagg tggcgacaat ttgcatatcg
      721 gcggcgacgg gaaagatggc gtctacgtgg tggtcgatgc gagcgacggg caggtaagtc
      781 tggcaaacaa taatagttat ttgggcacaa cacaaatcgc ctccggtacg ctgatggtga
      841 gcgacaactc gcagcttgga gatacccact ataaccgcca ggttatcttt accgataagc
      901 aacaagaaag cgtgatggag attactgcca atgtcgatac tcgctctacg acgactgagc
      961 atgggcgtga tattgaaatg cgcgccgacg gtgaagtggc agttgatgcg ggggtagaca
     1021 cgcagtgggg cgcactgatg gctgacagca gcgggcagca tcaggatgag ggtagcacat
     1081 ttactaaaac gggggcgggt acgctggagc tgaccgccag cggtacaacg cagtcggcag
     1141 tacgtgtcga agaaggcacc ctgaaaggcg atgttgcgga tatctttcct tatgcttcgt
     1201 cactgtgggt cggtgatggg gcggcgttcg ttactggcgc ggatcaggat attcagtcta
     1261 tcgatgctac ttccagcggc actatcgata tcagcgatgg tacggttttg cgtctgaccg
     1321 ggcaggatac ttccgtcgcc cttaatgcct cattgtttaa cggcgatggg acgctggtga
     1381 atgctaccga tggcgtgacg ttgacaggtg agcttaatac caaccttgaa actgacagcc
     1441 tgacctatct tgccgacgtg acggttaatg gtgatctgac caatacgtcc ggtgcggtta
     1501 gcctgcaaaa tggcgtcgct ggcgatacgc tgacggtaaa cggtgattat accggcggcg
     1561 gtacgctact gctcgatagc gaattaaacg gcgatgactc ggtaagcgat caattggtga
     1621 tgaacggtaa tactgctggc aataccacgg tggtggttaa ctctattaca gggattggtg
     1681 agccgacatc gacaggcatt aaagtggttg atttcgcagc tgatcccacg cagtttcaaa
     1741 acaatgcgca gttcagtctg gcaggcagcg gctacgtcaa tatgggagcg tatgactaca
     1801 cgctggtgga agataacaac gactggtatc tgcgatcgca agaagtaacg ccgccatcgc
     1861 cacctaatcc caacccgact cccgatcctg atcccacacc ggatcctgac ccaacccccg
     1921 acccggcccc tacgcctgct taccagccgg tgctgaatgc caaagttggc ggttatctca
     1981 ataacctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcac gctggtggcg
     2041 atggtcagac gctgaattta cgtgttatcg gcggacgtta tcattacacg gcggcagggc
     2101 aactggctca gcaggaagac acttctacgg tgcagcttag cggcggcttg tttagcgggc
     2161 gctggggcac ggatggcgag tggatgcttg gggcggttgg tagctacagt gataatcagg
     2221 gcgacagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac ggttatgccg
     2281 ttgggctgac ctcaagctgg tatcagcacg gtaatcagaa gcagggggcc tggctggata
     2341 actggttgca gtacgcgtgg tttagcaatg atgtttctga acatgaagat ggcacagatc
     2401 attaccattc gtcggggatt atcgcctcgc tggaagccgg gtatcagtgg ttaccggggc
     2461 gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag caggatgatt
     2521 ttaccgccgc taaccatgcg cgcgtgtcac aatcgcaggg ggatgatatt cagacgcggc
     2581 tgggtttaca cagcgaatgg cgtaccgcgg ttggtgtcac accaacatta gatctgaatt
     2641 attatcacga tccccatgcg acggaaattg aagaagatgg cagcactatc agtgacgatg
     2701 cggtgaagcg acggggtgaa ataaaagtgg gagtcacggg caatatcagt cagcgagtgt
     2761 ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag acggcagggt
     2821 ttttgtcgat gacggtgaaa tggtaatcgc tgtctgacca tcacaattgc cggatgcggc
     2881 gtaaacgcct tatctggcct acggagagca tttgtaggcc tgataagacg ctgcaagcgt
     2941 cgcatcaggc attggcgcgg ccaaacatca acctcactta ttctgcgtgt gcagcatata
     3001 gttcacatcc acgccggggc cgagcttaaa ggaattagtg atcgggttgt aatgtagccc
     3061 ggttatatgt cgctctttta aactggtctg atccacccag ccaagcagct ctgccggttt
     3121 aataaacttc ttcacatcat gcgtaccttt gggcaccatg cgcaaaatat attccgcgcc
     3181 aaccaccgcc atcagccatg acttgccgtt gcggttaagc gtcgagaaaa agacatcgcc
     3241 gcctggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg gtacgtgctc
     3301 cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg cgtgctcttc
     3361 cacggtttcc tgcacgtaat ccacctgaat gccgctttcc agcgcgtgca gctttgccac
     3421 ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc gcgccatact
     3481 ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa ataaaccgcc
     3541 agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcaacg gtttgaactc
     3601 accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct cttcgtggtc
     3661 tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc cgtaattggc
     3721 aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt acctcaaact
     3781 gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg gatagcggtt
     3841 agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag ctgaagagct
     3901 cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccggat gtccgagatg
     3961 gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc aatgactgga
     4021 acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa taccatcccc
     4081 atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc tcgctgcgtt
     4141 acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct gcggcggcaa
     4201 tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc gatctcgaaa
     4261 aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccg gacgtcatgc
     4321 caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta ggtatggcaa
     4381 ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg tatatcgatg
     4441 atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac ttcccgacgg
     4501 cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt cgtggcaagg
     4561 tgtacatccg cgcccgtgct gaagtggaag tcgacgccaa aaccgggcgt gaaaccatta
     4621 tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag attgcggaac
     4681 tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct gacaaagacg
     4741 gtatgcgcat cgtgattgaa gttaaacgcg atgcggtcgg tgaagttgtg ctcaacaacc
     4801 tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca ttgcaccatg
     4861 gtcagccgaa gatcatgaac ctgaaagata tcatcgcggc gtttgttcgt caccgccgtg
     4921 aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt gctcatatcc
     4981 ttgaagcact agccgtggcg ctggcgaaca tcgacccgat catcgaactg atccgtcatg
     5041 cgccgacgcc tgcagaagcg aaaactgcgc tggtggctaa tccgtggcag ctgggcaacg
     5101 ttgccgcgat gctggaacgt gctggcgacg atgctgcgcg tccggaatgg ctggagccag
     5161 agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag gcgattctgg
     5221 atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac gaatacaaag
     5281 agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat cgtctgatgg
     5341 aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa cgtcgtactg
     5401 aaatcaccgc caacagcgca gacatcaacc tggaagatct gatcacccag gaagatgtgg
     5461 tcgtgacgct ctctcaccag ggctatgtta aatatcagcc gctttctgaa tacgaagcgc
     5521 agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac tttatcgacc
     5581 gactgctggt ggcaaacacg cacgaccata ttctgtgctt ctccagccgt ggtcgcgtct
     5641 attcgatgaa agtttaccag ttgccggagg ccactcgtgg cgcgcgcggt cgtccgatcg
     5701 tcaacctgct gccgctggag caggacgaac gtatcaccgc gatcctgcca gtgaccgagt
     5761 ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg tactgtgaag aaaaccgtcc
     5821 tcaccgaatt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg gttgaaggcg
     5881 atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg ttctccgccg
     5941 aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc aacaccaccg
     6001 gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc gtgcctcgtg
     6061 gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc gcagtggcgg
     6121 aatacccaac caagtcgcgt gcgacgaaag gggttatctc catcaaggtt accgaacgta
     6181 acggtttagt ggttggcgcg gtacaggtag atgactgcga ccagatcatg atgatcaccg
     6241 atgccggtac gctggtacgt actcgcgttt cagaaatcag catcgtgggc cgtaacaccc
     6301 agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg caacgtgttg
     6361 ctgaaccggt tgacgaggaa gatctcgata ccatcgacgg cagtgccgcg gaaggggacg
     6421 atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa ttttacttct
     6481 tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct gcaaagtctg
     6541 ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa tggctggcta
     6601 tacacaagga atgtggcaat gagtggtgaa aaaaagcgaa aggctggcgg ttctatggtc
     6661 ttgtaggttt tggcgcaata gtactgcttt ccgctggtgt ctgggcgttg caatatgctg
     6721 gcagtgggcc agaaaaaacg ttgtcgccgc tggtggtgca caacaatctg caaatcgatc
     6781 tcaatgagcc ggacctcttt ctcgacagcg actctctgag ccagcttccc aaagatctcc
     6841 tcaccattcc gtttctccac gatgttctga gtgaagattt cgttttctat tatcagaatc
     6901 atgccgatcg tctgggcatt gaaggcagca ttcgtcgcat tgtctatgaa cacgatctca
     6961 cgctgaaaga taaactcttt tcgtcactct tagatcagcc cgcgcaggct gcgctatggc
     7021 acgataaaca aggccatctt tcacattaca tggtgctgat ccagcgcagt ggtttaagca
     7081 aactgctgga gccattgttg tttgccgcta ccagcgacag ccagttaagc aaaacggaaa
     7141 tcagtagcat caagataaat agtgaaacta ttcctgttta tcagttgcgc tataacggca
     7201 ataacgccct gatgttcgcg acttatcagg acaagatgct ggtgttttcc agcacggata
     7261 tgttgtttaa agatgatcag caggataccg aagccacggc gatcgcaggt gatttgttga
     7321 gcggcaaaaa acgctggcaa gcaagctttg gcctggaaga gcgtgctgct gaaaaaacgc
     7381 cagtacgcca gcgcatcgtg gtcagcgcca ggttgctggg ttttggctac cagcggttaa
     7441 tgccttcttt tgctggcgta cgcttcgaaa tgggtaacga cggctggcac agttttgtgg
     7501 cgttaaatga tgaatccgcc agcgtagata ccagtttcga ttttacgccg gtctggaaca
     7561 gtatgcctgc cggggccagc ttctgtgtgg tggtgccgta ttcacacggt attgccgaag
     7621 agatgctttc gcacatcagc caggaaaacg acaagctgaa tggggcgtta gacggtgccg
     7681 cggggctgtg ctggtatgaa gactcaaaat tgcaaacccc gctgtttgtc ggtcagtttg
     7741 atggcactgc cgaacaggcg caattgccag ggaaactgtt tacgcaaaat attggtgcgc
     7801 acgaaagcaa agcgccagaa ggtgttttgc cgataagcca gactcagcag ggcgaagcgc
     7861 aaatctggcg tcgcgaagtg agttcccgat acggtcagta tccgaaagcg caggcggcgc
     7921 aacccgatca attaatgtcg gattattttt tccgcgtgtc gctggcgatg caaaacaaaa
     7981 cgctgctttt ctccctcgat gacacgctgg ttaataacgc cctgcaaaca ctgaataaaa
     8041 accgcccggc aatggtggat gtaataccca ctgatggcat cgttccgctc tatatcaatc
     8101 cacaaggcgt ggcgaaactg ctgcgtaacg aaacgttgac cagtctgccg aagaatctcg
     8161 aaccggtttt ttataacgcc g
//
LOCUS       DAZOYS010000172         8175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOYS010000172 DAZOYS010000000
VERSION     DAZOYS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.173"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(361..1557)
                     /gene="hemY"
                     /locus_tag="ACHUN9_003381"
     CDS             complement(361..1557)
                     /gene="hemY"
                     /locus_tag="ACHUN9_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0696697.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1560..2759)
                     /gene="hemX"
                     /locus_tag="ACHUN9_003382"
     CDS             complement(1560..2759)
                     /gene="hemX"
                     /locus_tag="ACHUN9_003382"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0696698.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQVVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2781..3521)
                     /gene="hemD"
                     /locus_tag="ACHUN9_003383"
     CDS             complement(2781..3521)
                     /gene="hemD"
                     /locus_tag="ACHUN9_003383"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0696699.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3518..4459)
                     /gene="hemC"
                     /locus_tag="ACHUN9_003384"
     CDS             complement(3518..4459)
                     /gene="hemC"
                     /locus_tag="ACHUN9_003384"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0696700.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4846..7392
                     /gene="cyaA"
                     /locus_tag="ACHUN9_003385"
     CDS             4846..7392
                     /gene="cyaA"
                     /locus_tag="ACHUN9_003385"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0696701.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7432..7752)
                     /gene="cyaY"
                     /locus_tag="ACHUN9_003386"
     CDS             complement(7432..7752)
                     /gene="cyaY"
                     /locus_tag="ACHUN9_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0696702.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7914..8056)
                     /locus_tag="ACHUN9_003387"
                     /pseudo
     CDS             complement(7914..8056)
                     /locus_tag="ACHUN9_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1820 a         2167 c         2124 g         2064 t
ORIGIN      
        1 caagctcagg ttaaatattc actcaggaag caaagaggat tacagaatta tctcataaca
       61 agtgttaagg gatgttattt cccgattctc tgtggcataa taaacgggta gatgctcatt
      121 ccatctctta tgttcgcctt agtgcctcat aaactccgga atgacgcaga gccgtttacg
      181 gtgcttatcg tccactgaca gatgtcgctt atgcctcatc agacaccatg gacacaacgt
      241 tgagtgaagc acccactgtt gtcatacaga cctgttttaa cgcctgctcc gtaataagag
      301 caggcgtttt tttatgcatc agaaaggtcc cggaggtgct tgcctccggg tgagaaggaa
      361 ctactgtggt gggttgttct gcaacgttaa catcaaaccg tcgcgacgca tcgctgcagc
      421 ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat cgtaggcgtc
      481 cggacgttgt ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt ctccgtgctt
      541 catcagtgac tggcccagtg tgctccacaa cagcgggcga tcgccgacgt ttttgatctg
      601 ctggcgcagc actttttcca gctgttccgg attgttggtt ttcagtcgag gaatcggcag
      661 cagtaggcga tcgtcgtact ggcgtttcag gccatcgatg ataatttgct gggcagtatc
      721 atgatcgtca cattcaataa gatgttccgc cattgccacc tgcaacgcta cctgatgacg
      781 cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcactaccgt tatcggcacg
      841 cgcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat gttcttcatc
      901 accaacatgg gctttcgcca ttgatgggat gatatccagc agagaactcc atgcacctgt
      961 gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg taacttccag
     1021 cagcttatcc acgccgtggc gtgcagcatg gttttcatta cgggccagtt gcagacgtac
     1081 acgggtgatt tctaccggaa tggtgtcatt gccggccagc tctgctgcgc gttccagatg
     1141 ttggttggcg cgtgcttcat caccacgttg ttgcgcggct tcggcagcca gtagatagtt
     1201 caccaccggt tgttccgcgt gatcggcatt tttcgccatc agcttttcaa cttgctgata
     1261 atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgggcac ggcgacgctt
     1321 acgtccgaca aaccacccac gggtgtgtgc gcctgtgcgg aagatccgcc gcagtagcca
     1381 ctcaatggca aacagcacta ccatcgccag aatcaaaata atcgccaggc ccgtgacgct
     1441 ggtttcgata ttgtagttgt cagtctggat cagcacgtaa ccctgatgac cggcaatcat
     1501 cgggccaacc actatccccg caatcagcaa aacaaagagt aataacactt ttagcatggt
     1561 tattctcctt gcggcgcggc tgccggagta tcaccttgcg gtgcaggtgc aggtgcaggt
     1621 gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt gtgccagcag gttacgcacg
     1681 cgggtctgca tcagtttttc cagcatcgcc tggctttgca gggtttccgg aagatccatc
     1741 gagatatttt gctggcttaa ctggtccacc tcgtcgagga acgctttggt ggtggcatca
     1801 tcagtatcgt agtaagcacg tacccaggtg gagacgttct ccagcgcctg gcgataagtc
     1861 tcttcctgat gacgcggtac agcttgtgct gcgaccagca ggcgagagcg aatattttcg
     1921 cgcagataga tatcctgatt tggcgctaac agcggtacgg cggtgtcatc acgacggcga
     1981 atcgtaatga agttgtccat aaagttctgc cagctttttt gcagattgat acgccattcg
     2041 ctgatggaac tggaaagctc ttcaccgtct gaatccatcg gcgaaccatc gctgtcatta
     2101 tcggccagac gcaggttatc tacctgattt gaaagctgat taagcttaag gatgatgccg
     2161 tcataatcca cctgcgatac tgcagaaagg ctggcgatat catcggtaat tgcccgacga
     2221 acggtaatca gactcgggtc attcatatcc gccaggctgg cgtctgcact tttcagcaac
     2281 gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc gtccggcgag tttcaccaga
     2341 aaatcggcct gggccagcag ccaggtttta gcatcgctgc cggaaatggt ggcgaccttt
     2401 tgctggactt catccagctg ttttgccagc gtttcttgct gacgattcgc ctgttcaagc
     2461 tgcgtagcct gttgcttaat aatgccttcc agttcggctt tttggctctc ctgggctttt
     2521 tgcaatgccg tcagttggtt agccagggca tcgctggtgg cggtctgatt gacgacctgt
     2581 tgtttacccc agccatacaa accgacgccc gccgccagag caatagcgat agccgccgcg
     2641 ctgagaatca atgcggtatt gttcttactc tttttttctg ttgcgacagg ttgtgacgtg
     2701 gtgtccacgg cctccctagt ctcttcaacc acggcggagg ttttttcttg ttccgtcatt
     2761 atggcttcct gttatgagag ttattgtaat gcccgtaaaa gcgcatcgtt ggcagcgtta
     2821 tcggcgacct taatgtcttg ccagcccagt tcccgggcga gtttcgccaa acgctcactg
     2881 acgaccaata gtcgacagtg tagtaaccag tgctcacgat accattgtgg gatcagcgac
     2941 cagagttgct gcaacatttc accgctggta acaacgaccg tcgtcacctc gcgggattgc
     3001 cagcgcatcg cttcttctgc accatcgtaa tggattgcgc atcgttgata acattcacaa
     3061 aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa ttagctcacg accgccattg
     3121 ccacgtaata tcagcgcacg tttgcccgca atattttgta attcaggtaa ttgtagcaag
     3181 acttcgctga tttcccgatc ctgcgggtag agaatcttct gtccgcttac ggtatgtagt
     3241 gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta gtcggggcca tttacgatct
     3301 tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt gcgagagggc aaacaacaga
     3361 tcgctctccc ccagcgctgc cagttgatca gcaagttgcg gtaattgttg acccggagaa
     3421 aactcaatca gcggaaaatg ccaggccacc tgccccagtg tgcgcagacg gctcactaac
     3481 tcttctccag cgggagacgg gcgggtgaca aggatactca tgccggggcg tctccgttat
     3541 agacttcagc gaggatctcg cgcgcgccgt tattcagtag ctcttctgcc agcgaaatcc
     3601 ccatttgttt ggcattttgc ggcgcaccgc ggcgttcacc gcgaataatc tgcgaaccgt
     3661 ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc aataagctcg gcgtagctac
     3721 caattggcac ctgacatccg ccttcgagac gggtattcat ggcgcgttct gcggtaacgc
     3781 gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc gcgagtgcgc gtatcatcaa
     3841 ggcggcattc aataccgact gcaccttgtc ctaccgccgg aagagaaatc tcgggtggta
     3901 acgcggcgcg aatgcgggac tccagaccta aacgttttag tccggctacg gcaagaatga
     3961 tggcatcgta ttcgccgtta tctagtttgc tcaggcgagt gccgacgttg ccgcgcaggg
     4021 agcggataat cagatccggg cggcgttcag ccagttggca ctggcgacgt aaactggacg
     4081 tcccgacgat actgcctgct ggtaacgcat ccagactgtc atagttattg gacacaaagg
     4141 catcgcgagg atcttcacgc tcacaaatag tgaccagtcc cagaccttgc gggaattcaa
     4201 ccggcacatc tttcattgaa tgtacggcga tatcggcgcg attttcgagg agcgcgactt
     4261 ccagctcttt tacaaataag ccttttccgc ctactttcgc cagcggcgta tcaagaatca
     4321 catcgccgcg cgtcaccatc ggtaccagtt caacgaccag gcccggatgg ctcgccatca
     4381 acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg gctttggcgt gtggcaattc
     4441 ttaaaacatt gtctaacatg cttgttaccg tcattatcat ccgtggtcca tcctaacatc
     4501 cttgccagag tgatgtcagt gttgtggtga aacgtagacg cctgcgcaaa ccgtaaaatg
     4561 aggtctggca gtggatcctg acagacgttt cacgctgtcg taataaggaa tttacagaga
     4621 ataaacggtg ctacacttgt atgtagcgca tctttcttta cggtcaatca gcaaggtgtt
     4681 aaattgatca cgttttagac cattttttcg tcgtgaaact aaaaaaacca ggcgcgaaaa
     4741 gtggtaacgg ttacctttga catacgaaat atcccgaatg ccgcgtgtta ccgttgatgt
     4801 tggcggaatc acagtcatga cgggtagcaa atcaggcgat acgtcttgta cctctatatt
     4861 gagactctga aacagagact ggatgccata aatcaattgc gtgtggatcg cgcgcttgct
     4921 gctatggggc ctgcattcca acaggtctac agtctactgc cgacattgtt gcactatcac
     4981 catccgctaa tgccgggtta ccttgatggt aacgttccca aaggcatttg cctttacacg
     5041 cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac tgtatcgtgg aatgtcagta
     5101 caggatccac cgaaaggtga gcttccaatt actggtgtat acaccatggg cagcacctcg
     5161 tccgtagggc aaagttgttc ctctgacctg gatatctggg tctgtcatca atcctggctc
     5221 gatagcgaag agcgccagtt actacaacgt aaatgcagcc tgctggaaag ctgggccgcc
     5281 tcgctgggtg tggaagtcag cttcttcctg attgatgaaa accgctttcg tcataatgaa
     5341 agcggcagcc tggggggcga agattgtggc tccacccagc atatactgct gcttgacgaa
     5401 ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc tgtggaatat ggtgccgtgc
     5461 gacgaagaag agcattacga cgactatgtg atgacgcttt acgcgcaggg cgtgctgacg
     5521 ccaaatgaat ggctggatct cggtggctta agctcgcttt ctgctgaaga gtactttggt
     5581 gccagccttt ggcagctcta caagagtatc gattccccat acaaagcggt gctgaaaaca
     5641 ctgctgctgg aagcctattc ctgggaatac ccgaacccac gtctgctggc gaaagatatc
     5701 aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc tcgatccata ctgcatgatg
     5761 ctggagcgtg ttactgaata cctgacggcg attgaagatt tcacccgtct ggatttagta
     5821 cgtcgctgct tctatttaaa agtgtgcgaa aaactcagcc gtgaacgcgc ctgtgtaggc
     5881 tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg gttgggacga agctcgtctg
     5941 gcaatgctcg ataaccgtgc taactggaag attgatcagg tgcgtgaggc gcacaacgag
     6001 ttgctcgacg cgatgatgca gagctaccgt aatctgatcc gctttgcgcg tcgcaataac
     6061 cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga cgcgtaagct gtatgccgcg
     6121 tttgaagcat taccaggtaa agtgacgctg gtaaacccgc agatttcacc cgatctctcg
     6181 gaaccgaatc tgacctttat ttatgtgccg ccgggccggg ctaaccgttc aggttggtat
     6241 ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc atcagccgct ggaatataac
     6301 cgttacctga ataaactggt ggcgtgggca tggtttaacg gtctgctgac ctcgcgcacc
     6361 cgtttgtata ttaaaggtaa cggcattgtc gatttgccta agttgcagga gatggtcgcc
     6421 gacgtgtcgc accatttccc gctgcgctta cctgcaccga caccgaaggc gctctacagc
     6481 ccgtgtgaga tccgccatct ggcgattatc gttaacctgg aatatgaccc gacagcggcg
     6541 ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg atgtcttcag ctttggcgag
     6601 aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc gcaactcgtg gaacgaagtg
     6661 cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag ccctgaaaac tattctcggc
     6721 aaaatgcatc aggacgccgc accgccagat agcgtggaag tcttctgtta tagccagcat
     6781 ctgcgcgggt taatccgtac tcgcgtgcag caactggttt ctgagtgtat tgaactgcgc
     6841 ctttccagca cccgccagga aaccgggcgt ttcaaggcgc tgcgcgtttc tggtcaaacc
     6901 tggggcttgt tcttcgaacg cctgaatgta tcggtacaga agctggagaa cgccatcgag
     6961 ttttatggcg ctatttcgca taacaaactg cacggcctgt cagtgcaggt tgaaaccaat
     7021 cacgtcaaat taccggcggt ggtggacggc tttgctagtg aagggatcat ccagttcttc
     7081 ttcgaagaaa cgcaagacga gaatggcttt aatatctaca ttcttgacga aagcaaccgg
     7141 gttgaggtgt atcaccactg cgaaggcagc aaagaggaac tggtgcgtga cgtcagtcgc
     7201 ttctactcgt catcgcacga ccgcttcacc tacggctcaa gcttcatcaa cttcaacctg
     7261 ccgcagttct atcagattgt gaaggttgat ggtcgtgaac aggtgattcc gttccgcaca
     7321 aaatctatcg gtaacatgcc gcctgccaat caggatcacg atacgccgct attacagcaa
     7381 tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc ggcatgcaat cttagcggaa
     7441 actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc agcaaatccc agaaggtttc
     7501 gccgctgcga tcgcaaatcc actcatcgcc tttcagatca aaatggtagc cgccttgttt
     7561 ggttgccagc catacctggt gcagcggttc ctggcggttg ataatgattt tgctgccgtt
     7621 ttcaaaggta atggtcagca cgccgccgtt gatttcgcag tcgatatcgc tgtcgccatc
     7681 ccagtcgtcc aggcgttctt caatggtcag ccagagttga tcagccaggc gatgaaattc
     7741 actgtcgttc attgttgtat cctgttttta agtgatggcg gcagtatagc ggcatggggt
     7801 cagggcttca aagtttgcac ctctgcggct gcgttccggc acgattcatc cgtcaccgga
     7861 ataatgatgt ctctgtgtag cgaaagattt gtctcttcat tagggcgcag ttacaccacg
     7921 cctttccctg tttctggtaa acattatgat caggtttacc gagcgagcat cctcacgctg
     7981 acggaactaa aaaagacaac aaacaaacca cattgcgata gtgcatagag ccatcctggc
     8041 gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc cccacgagta agcgttatac
     8101 tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt gcttttacgt
     8161 cttctcctgc gatga
//
LOCUS       DAZOYS010000173         8156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOYS010000173 DAZOYS010000000
VERSION     DAZOYS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.174"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHUN9_003388"
     CDS             <1..254
                     /locus_tag="ACHUN9_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0696703.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUN9_003389"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUN9_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0696704.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUN9_003390"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUN9_003390"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0696705.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHUN9_003391"
     CDS             complement(2905..3180)
                     /locus_tag="ACHUN9_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0696706.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUN9_003392"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUN9_003392"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0696707.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUN9_003393"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUN9_003393"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0696708.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5465
                     /locus_tag="ACHUN9_003394"
                     /pseudo
     CDS             5140..5465
                     /locus_tag="ACHUN9_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
     gene            5649..6368
                     /locus_tag="ACHUN9_003395"
     CDS             5649..6368
                     /locus_tag="ACHUN9_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0696709.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6544..7590
                     /gene="ansB"
                     /locus_tag="ACHUN9_003396"
     CDS             6544..7590
                     /gene="ansB"
                     /locus_tag="ACHUN9_003396"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0696710.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7707..>8156
                     /locus_tag="ACHUN9_003397"
     CDS             7707..>8156
                     /locus_tag="ACHUN9_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0696711.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1987 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaaag cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     5221 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     5281 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     5341 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     5401 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     5461 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     5521 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     5581 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     5641 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     5701 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     5761 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     5821 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     5881 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     5941 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     6001 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     6061 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     6121 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     6181 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     6241 tgcaatcctc aatccaaaca gagtggaaaa agcaggaaaa agatttccag cagtttggca
     6301 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     6361 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     6421 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     6481 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     6541 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     6601 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     6661 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     6721 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
     6781 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
     6841 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
     6901 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
     6961 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt gaccgcagct
     7021 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
     7081 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
     7141 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
     7201 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
     7261 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
     7321 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
     7381 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
     7441 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
     7501 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
     7561 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
     7621 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
     7681 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
     7741 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
     7801 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
     7861 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
     7921 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
     7981 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
     8041 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
     8101 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattat
//
LOCUS       DAZOYS010000174         8152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOYS010000174 DAZOYS010000000
VERSION     DAZOYS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.175"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUN9_003398"
     CDS             <1..188
                     /locus_tag="ACHUN9_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696712.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUN9_003399"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUN9_003399"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0696713.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUN9_003400"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUN9_003400"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0696714.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUN9_003401"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUN9_003401"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0696715.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUN9_003402"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUN9_003402"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0696716.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUN9_003403"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUN9_003403"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0696717.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUN9_003404"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUN9_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0696718.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUN9_003405"
     CDS             7142..7351
                     /locus_tag="ACHUN9_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0696719.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUN9_003406"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUN9_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0696720.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1843 a         1989 c         2224 g         2096 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg ag
//
LOCUS       DAZOYS010000175         8086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOYS010000175 DAZOYS010000000
VERSION     DAZOYS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.176"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            37..558
                     /gene="fecI"
                     /locus_tag="ACHUN9_003407"
     CDS             37..558
                     /gene="fecI"
                     /locus_tag="ACHUN9_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0696721.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            555..1508
                     /gene="fecR"
                     /locus_tag="ACHUN9_003408"
     CDS             555..1508
                     /gene="fecR"
                     /locus_tag="ACHUN9_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0696722.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1595..3919
                     /gene="fecA"
                     /locus_tag="ACHUN9_003409"
     CDS             1595..3919
                     /gene="fecA"
                     /locus_tag="ACHUN9_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0696723.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            3964..4866
                     /gene="fecB"
                     /locus_tag="ACHUN9_003410"
     CDS             3964..4866
                     /gene="fecB"
                     /locus_tag="ACHUN9_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0696724.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4863..5861
                     /gene="fecC"
                     /locus_tag="ACHUN9_003411"
     CDS             4863..5861
                     /gene="fecC"
                     /locus_tag="ACHUN9_003411"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0696725.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5858..6814
                     /gene="fecD"
                     /locus_tag="ACHUN9_003412"
     CDS             5858..6814
                     /gene="fecD"
                     /locus_tag="ACHUN9_003412"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0696726.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6815..7582
                     /gene="fecE"
                     /locus_tag="ACHUN9_003413"
     CDS             6815..7582
                     /gene="fecE"
                     /locus_tag="ACHUN9_003413"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0696727.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1728 a         2333 c         2286 g         1739 t
ORIGIN      
        1 atggctacgt gataattaac ttttgatgca ctccgcatgt ctgaccgcgc cactaccaca
       61 gcttccttaa cattcgagtc gctttatggc acacatcacg gctggttgaa aagctggctg
      121 acgcgcaaac tccagtctgc ttttgatgca gatgacattg cccaggacac ttttttgcgg
      181 gtaatggtca gcgaaacgct ctcgacgatc cgcgatcctc gctccttcct ctgcactatc
      241 gccaaacgcg tgatggtgga cctgtttcgc cgaaacgcgc tggaaaaagc gtatctggag
      301 atgctggcgc ttatgccgga ggggggagcg ccttcacctg aggaacgcga aagccaactc
      361 gagaccctac aactcctcga cagcatgctg gacgggctaa acggcaaaac acgtgaagcg
      421 tttctgcttt cgcaactgga tggtctgaca tacagcgaga ttgcgcacaa actcggtgtt
      481 tccatcagct ccgtgaaaaa atacgtggcg aaagccgtcg agcactgcct gctgttccgt
      541 ctggagtatg ggttatgaat cctttgttaa ccgattcccg ccgtcaggcg ctgcgttcag
      601 cttcccactg gtatgccgtg ctaagcggcg agcgcgtcag cccacaacag gaagcgcgct
      661 ggcaacagtg gtatgaacag gatcaggata accagtgggc ctggcagcag gttgaaaacc
      721 tgcgcaacca gcttggcggt gtgcctggcg acgttgccag ccgggcgttg cacgataccc
      781 gcctcacccg ccgtcacgtg atgaaaggat tactgctgtt gctcggcgct ggcggaggct
      841 ggcagctctg gcagtcggaa accggcgaag gtctgcgggc agattaccgc accgccaaag
      901 gcacagtcag ccgtcagcaa ctggaagatg gctccctgct cacgctgaat acccaaagcg
      961 cggcggatat gcgttttgat gcgcatcagc gcaccgtccg gctctggtac ggtgaaatcg
     1021 ccattactac cgcgaaagat gccctgcaac gccccttccg tgtcctgacc cgtcagggcc
     1081 agctcactgc tttagggaca gaatttaccg tccgccagca ggataatttc acgcagcttg
     1141 acgtgcagca gcacgccgtg gaagtgcttc tcgccagtgc ccccgcgcaa aaacgcatcg
     1201 tgaacgctgg tgaaagcctg cagttcagcg cctctgagtt tggcgcagtg aaaccgctgg
     1261 atgacgagag tacaagctgg acgaaggaca tcctgagctt cagcgataaa ccgctgggtg
     1321 aggtgatagc cacgctaacc cgttaccgca acggcgtgct gcgctgcgat cccgccgttg
     1381 ccgggctgcg cctgagcggg acgttcccgc tgaaaaatac cgatgcgatc ctgaacgtta
     1441 tcgcgcaaac gcttcccgtt aaaattcagt ctattacgcg gtactggata aacatttcac
     1501 cactgtaagg aaaataattc ttatttcgat tgtccttttt acccttctcg ttcgactcat
     1561 agctgaacac aacaaaaatg atgatgggga aggtatgacg ccgttacgcg tttttcgtaa
     1621 aacaacacct ttggttaacg ccattcgcct gagcctgctg ccgctggccg gtctctcgtt
     1681 ttccgctttt gctgcacagg ttaatatcgc accgggatcg ctcgataaag cgctcaatca
     1741 gtatgccgca cacagcggat ttaccctctc ggttgacgcc agcctgacgc gcggcaagca
     1801 gagcaacggc ctgcacggcg attacgacgt cgagagcggc ctgcaacaac tgctggacgg
     1861 cagcggactg caggtaaaac cgctgggaaa taacagctgg acgctggagc ccgcgcccgc
     1921 accaaaagaa gatgccctga ccgtggtcgg cgactggctg ggtgatgcgc gtgaaaacga
     1981 cgtatttgaa catgctggcg cgcgtgacgt gatccgccgt gaggatttcg ccaaaaccgg
     2041 cgcaaccacc atgcgtgagg tacttaaccg catccctggc gtcagcgcgc cggaaaacaa
     2101 cggcaccggc agccacgacc tggcgatgaa ctttggcatc cggggcctga acccgcgcct
     2161 cgccagccgc tcgaccgtcc tgatggacgg catccccgtc cccttcgccc cttacggtca
     2221 gccgcagctt tcactggctc ccgtttcgct cggcaacatg gatgccattg acgtggtacg
     2281 cggtggtggt gcggtgcgtt acggaccgca gagcgtgggc ggcgtggtga actttgttac
     2341 ccgtgccatt ccgcaggact ttggtatcga ggcgggcgtg gaaggtcagc tcagcccaac
     2401 ctcttcacaa aacaacccga aagagacgca caacctgatg gtgggcggca cagcggacaa
     2461 cggttttggc accgcgctgc tctactccgg cacgcgcggc agtgactggc gcgagcacag
     2521 cgccacccgc atcgacgacc tgatgctgaa aagcaaatat gcgccggatg aggtgcacac
     2581 cttcaacagc ctgctgcaat attacgacgg tgaagccgac atgcccggtg gcctgtctcg
     2641 cgcggattac gacgccgatc gctggcaatc cacccgcccg tatgaccgct tctggggtcg
     2701 tcgcaagctg gcgagcctgg gctaccagtt ccagccagac agccagcata aattcaacat
     2761 tcaggggttc tacacccaaa ccctgcgcag cggctacctg gagcaaggca aacgcatcac
     2821 cctctcgccg cgtaactact gggtgcgcgg tattgagcca cgctacagcc agatctttat
     2881 gatcggccct tccgcgcacg aagtgggcgt gggctatcgc tatgtgaatg aatcaacgca
     2941 tgaaatgcgt tactacaccg ccaccagcag cgggcagttg ccgtccggct caagccctta
     3001 cgaccgcgat acgcgttccg gcaccgaggc gcacgcctgg tatctggatg acaaaatcga
     3061 catcggcaac tggaccatca cgccgggtat gcgtttcgaa catatcgagt cataccagaa
     3121 caacgccatc acaggcacgc acgaagaagt gagctataac gcaccgcttc cggcgttgaa
     3181 cgtgctctat cacctgactg acagctggaa tctttatgca aacactgaag gctcgttcgg
     3241 caccgtacag tacagccaga ttggcaaggc tgtgcaaagc ggcaatgtgg aaccggaaaa
     3301 agcgcgaacc tgggaactcg gtacccgcta cgacgacggc gcgctgacgg cggaaatggg
     3361 gctgttcctg attaacttta acaatcagta cgactccaac cagaccaacg acaccgtcac
     3421 tgcacgtggc aaaacgcgcc ataccgggct ggaaacgcag gcacgttacg atctgggtac
     3481 gctaacgcca acgcttgata acgtttccat ctacgccagc tatgcgtatg tgaacgcgga
     3541 aatccgcgag aaaggcgaca cctacggcaa tctggtacca ttctccccga aacataaagg
     3601 cacgctgggc gtggactaca agccaggaaa ctggatgttc aatctgaaca gcgatttcca
     3661 gtccagccag tttgcggata acgccaatac ggtgaaagag agcgccgacg gcagtaccgg
     3721 ccgcattccc ggcttcatgc tctggggcgc acgcgtggtg tatgactttg gcccgcagat
     3781 ggcagatctg aacctggcat tcggtgtgaa aaacatcttc gaccaggact acttcatccg
     3841 ctcttatgac gacaacaaca aaggcatcta tgcaggccag ccgcgcacgc tgtatatgca
     3901 gggggcgttg aagttctgat gtgttaacgc ccggcttgcc gggcttttag ctggaatgtg
     3961 attatgttgg catttatccg ttttcttttt gcaggcctgc tgctggtgat cagccacgcc
     4021 tttgccgcca cggttcagga cgaacacggc acgtttacac tcgaaaaaac gccacaacgg
     4081 attgtggtgc tggaactctc gttcgccgat gcgctggccg ccgtggacgt cagcccgatc
     4141 ggtattgccg acgataacga tgcaaaacgc atcctgcccg aagtgcgtgc gcacctgaaa
     4201 ccgtggcagt ccgtcggaac gcgcgcgcag ccgagcctgg aagccattgc cgctctgaaa
     4261 ccagacctga tcattgccga cagcagtcgc catgcggggg tttacatcgc cttgcagcaa
     4321 atcgcgtcgg tactgctgct taagtcccgc aacgaaacct acgctgaaaa tttgcaatct
     4381 gcggctatca tcggcgaaat ggtgggtaaa aagcgagaga tgcaggcacg tctggaacaa
     4441 cataaagaga ggatggcgca gtgggccagc cagcttccca aagggacacg cgtggccttt
     4501 ggcacatcac gcgaacagca attcaacctg catactcagg agacctggac cggcagcgtg
     4561 ctggcctctc tggggctgaa cgttcccgct gcgatggcgg gtgcgtccat gccgtccatc
     4621 ggcctggagc aactgctggc ggtcaatcct gcctggctgc tggttgccca ctatcgcgaa
     4681 gagagcattg ttaaacgctg gcaacaagat ccgctctggc agatgttaac cgccgcgcag
     4741 aagcagcagg ttgcttcggt cgacagtaac acctgggcgc ggatgcgcgg tatttttgct
     4801 gcagagcgta ttgccgctga cacggtaaaa atcttccacc atcagccgct taccgttgtg
     4861 aaatgaccgc gataaaacac ccggtgctgc tgtgggggct tcccgttgca gcacttatta
     4921 ttattttctg gctgagtctg ttttgctact cggccattcc tgtttccgga gcagatgcaa
     4981 cccgcgccct gctgcctgga cacacgccaa cgctaccaga agcgctggtg caaaaccttc
     5041 gtttgccacg aagcctggtc gccgttctga tcggcgcaag cctggcgctc gcgggcacgc
     5101 tgctgcaaac cctgacccac aacccaatgg cctctccttc actgctcggc attaacagcg
     5161 gcgcggcgct ggctatggcg cttaccagcg cgctgagtcc gacgccgatt gcaggctatt
     5221 ctctgtcgtt catcgcggca tgcgggggcg gcgtgagctg gctgctggtc atgaccgcag
     5281 gaggcggatt tcgtcatacc catgacagaa acaaactgat cctcgcgggt atcgcgctgt
     5341 cggccttttg tatgggcctt acccgcatca ccctgctgct ggccgaagat catgcttacg
     5401 gcatctttta ctggctggct ggcggagtgt cccacgcccg ctggcaggat gtctggcagc
     5461 tcttgccggt ggtggtcact gcagtccctg tcgtgttgct gctggcgaat caactgaacc
     5521 tgctcaacct cagcgacagc accgcccata cgctgggagt gaacctgacg aggctacgtt
     5581 tggtcatcaa tatgttagtg ctgcttctgg ttggtgcgtg cgtcagcgtg gcaggtccgg
     5641 tggcgtttat cggtctgctg gtgccacatc tggcgcgctt ctgggcaggc ttcgatcagc
     5701 gcaacgtact gccagtgagc atgctgctgg gggccacgct gatgctgctg gcagatgtac
     5761 tcgcacgcgc gctggccttc cccggagatc tgcccgcagg cgcagtgctg gcgctgattg
     5821 gcagcccttg ctttgtctgg cttgtgagga ggcgaggatg aaaattgcgc tggttatttt
     5881 catcaccctt gccctggcgg gctgtgcgct gttatcactc catatgggag tgatccccgt
     5941 gccgtggcgc gcgctgctga ccgactggca ggccggacgc gagcattatt atgtattgat
     6001 ggagtaccga ctgccgcgct tgctgctggc actgtttgtc ggtgcagccc tcgccgtggc
     6061 gggcgtgctg atacagggga ttgtgcgcaa ccctctggca tcaccggata ttctcggcgt
     6121 taaccatgcc gccagcctgg cctctgtggg ggctctactt cttatgccgt cactgcccgt
     6181 gatggtgctg ccgctgctgg cctttgcggg cggcatggcg gggttgatat tgctgaagat
     6241 gctggcaaag acccaccagc cgatgaagct ggcactcacc ggcgtggcgc tttctgcatg
     6301 ctgggccagc ctgacggatt atctgatgct ctcgcgcccg caggatgtga acaacgccct
     6361 gctgtggctg accggcagct tatggggccg tgactggagc tttgtgaaga ttgccatccc
     6421 gctgatgatt ttatttctgc cgctgagcct gagtttttgc cgcgatctcg acctccttgc
     6481 actcggcgat gcgcgcgcca ccacgctcgg tgtgtcggtg ccctataccc gattctgggc
     6541 tttgttacta gctgtcgcca tgacatctac cggcgtggcc gcctgcggcc cgattagctt
     6601 tattggtctc gtggtgccgc atatgatgcg tagcatcacc ggtggacgtc accgcagact
     6661 gctgcctgtt tcggccctga caggtgcgtt gctgttggtg gttgccgatc tgctggcgag
     6721 aattattcat cccccactgg agctcccggt tggcgtgctg accgccatta tcggtgcgcc
     6781 gtggtttgtc tggttgcttg tgagaatgcg ataaatgact ttacgaactg aaaatctgac
     6841 ggtcaattac gggacagaca aggtacttaa cgacgtttca ctctcactgc caacggggaa
     6901 gatcaccgcc ctgatcggtc ctaacggttg cgggaaatcg acgctgttaa actgtttttc
     6961 gcggctttta atgccgcagt ctggcaccgt atttctcggc gataatccca taaatatgct
     7021 ctcatcgcgc cagttggccc gcaggctttc gctgctgcct cagcaccatt taacgccaga
     7081 ggggatcaca gtccaggagc tggtttcgta tggtcgtaat ccctggctgt cactctgggg
     7141 gcgtctctcc gctgaagaca atgcacgagt taatgtcgcc atgaaccaga cccggatcaa
     7201 tcatcttgcc gttcgtcggt taaccgagct ttccggcggt cagcgccagc gcgcatttct
     7261 ggcaatggtc ctggcccaga atacgcccgt tgtattactt gatgagccaa ccacctatct
     7321 tgatatcaat caccaggtgg acctgatgcg gttgatgggc gaactccgga ctcaggggaa
     7381 aacggtggtc gctgtgctgc acgaccttaa tcaggctagc cggtactgcg atcaactggt
     7441 ggtaatggca aacggacatg ttatggcgca aggcacacca gaagaggtga tgaccccagg
     7501 attgctgaga acagtattca gcgtggaagc ggaaatacac cccgagccgg tatctggcag
     7561 gccgatgtgc ctaatgaggt agattgcaca ggccgtaaga accaaaccac gactgaatga
     7621 aactggactg gcgccagcaa gcctgttcag actggggctg aacttttccg gactctgaaa
     7681 gattaccaat actcatcgtc catccgcttg ctttaggctg acaggttcat aatcaacgca
     7741 aaccagagct gtacaggctt gggcgcggct ttcaaaccag tcgtgatcac ggcaatcaat
     7801 tttgaactct gcttaacgga catttctgta taacccttac ggcaacgaaa aacgcgaagt
     7861 taaaatttta gaaacccaaa aacgtgacat gactaagttt agatttcagg ggggggagat
     7921 caaaaaattt cgctctgtgc cagagcggac attcacggag ctggttcatt accaatgagg
     7981 ttgggctttt gaggataaat caatgatcag acgccaacgt aaatcaaaag caaccctgga
     8041 acggaattgc taatccagtt tctgaccatc gatttttcta aaaagt
//
LOCUS       DAZOYS010000176         8037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOYS010000176 DAZOYS010000000
VERSION     DAZOYS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.177"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            23..88
                     /gene="yobI"
                     /locus_tag="ACHUN9_003414"
     CDS             23..88
                     /gene="yobI"
                     /locus_tag="ACHUN9_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR0696728.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            227..838
                     /gene="ruvA"
                     /locus_tag="ACHUN9_003415"
     CDS             227..838
                     /gene="ruvA"
                     /locus_tag="ACHUN9_003415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0696729.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            847..1857
                     /gene="ruvB"
                     /locus_tag="ACHUN9_003416"
     CDS             847..1857
                     /gene="ruvB"
                     /locus_tag="ACHUN9_003416"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0696730.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2004..2789)
                     /gene="znuB"
                     /locus_tag="ACHUN9_003417"
     CDS             complement(2004..2789)
                     /gene="znuB"
                     /locus_tag="ACHUN9_003417"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0696731.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2786..3541)
                     /gene="znuC"
                     /locus_tag="ACHUN9_003418"
     CDS             complement(2786..3541)
                     /gene="znuC"
                     /locus_tag="ACHUN9_003418"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0696732.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3620..4552
                     /gene="znuA"
                     /locus_tag="ACHUN9_003419"
     CDS             3620..4552
                     /gene="znuA"
                     /locus_tag="ACHUN9_003419"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0696733.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4568..5890
                     /gene="mepM"
                     /locus_tag="ACHUN9_003420"
     CDS             4568..5890
                     /gene="mepM"
                     /locus_tag="ACHUN9_003420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0696734.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6010..6981
                     /gene="lpxM"
                     /locus_tag="ACHUN9_003421"
                     /gene_synonym="msbB"
     CDS             6010..6981
                     /gene="lpxM"
                     /locus_tag="ACHUN9_003421"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0696735.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7069..8037)
                     /locus_tag="ACHUN9_003422"
                     /pseudo
     CDS             complement(7069..8037)
                     /locus_tag="ACHUN9_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2142 a         1920 c         2022 g         1953 t
ORIGIN      
        1 ttattttttt tcaggaggcg caatgtatat ttttattacg catttattca ctgaatatgt
       61 aatattaaaa tatttgcttc caatataacc tgtagaataa attatactgt gccatttttc
      121 agttcatcga gacacctcgc aagttttctt catccttcgc tggatatcta tccagcattt
      181 ttttatcata cagcattatc tttgattcat tacgcaggag cgtcatgtga taggcagact
      241 cagaggcatc atcattgaaa aacaaccccc gctggtgtta attgaagtgg gcggcgtagg
      301 ctatgaagtg catatgccga tgacctgttt ttatgaactc cctgaagcgg gtcaggaagc
      361 gatcgttttc acccactttg tggtgcgtga agacgcgcaa ctgctgtacg gttttaacaa
      421 taaacaagag cgcacgttgt ttaaagagtt aatcaaaacc aacggcgtcg gcccgaagtt
      481 ggcgctggcg atcctctccg gaatgtcagc gcagcagttc gttaatgccg ttgaacgtga
      541 agaagtggga gcactggtga aactgccggg cattggtaaa aaaaccgccg agcgtttaat
      601 tgtcgaaatg aaagaccgat ttaaaggttt gcacggcgat ctctttacgc cagccgccga
      661 tctggtgctc acgtcgcctg ccagcccggc aaccgacgat gctgaacagg aagcggttgc
      721 cgcgctggtg gcgctgggct ataaaccaca agaagcaagc cgcatggtga gcaaaatcgc
      781 tcgcactgac gccagcagtg aaacattaat tcgcgaagcc ctacgcgccg cgttatgagg
      841 taaaggatga ttgaagcaga ccgtctgatt tctgccggta ccactttgcc ggaagatggg
      901 gcagatcgcg ccattcgccc taaattactg gaagagtatg ttggtcagcc gcaggttcgt
      961 tcacagatgg agattttcat caaagcagcg aaactgcgcg gcgatgccct cgatcatctg
     1021 ttgatttttg gtcctccggg gttgggtaaa actacgcttg ccaacattgt cgccaatgaa
     1081 atgggcgtta atttacgcac gacttctggt ccggtgctgg aaaaggcggg cgatctggca
     1141 gcgatgctca ctaaccttga accgcatgac gtgctgttta ttgatgagat ccaccgtcta
     1201 tcgccagttg ttgaagaagt gctgtacccg gcaatggaag actaccaact ggatatcatg
     1261 attggtgaag gtccggcggc acgctccatt aaaattgatt tgccgccgtt taccctgatt
     1321 ggtgcaacca cgcgcgcagg ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg
     1381 caacgtctgg agttttatca ggtgccggat ctgcaatata tcgtcagtcg cagcgcacgc
     1441 tttatggggc ttgagatgag tgatgacggc gcgctggaag ttgctcgtcg cgctcgcggt
     1501 acgccacgta ttgccaaccg tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac
     1561 gatggcacca tctcggcaga tatcgctgct caggcgctgg atatgttgaa tgtcgatgct
     1621 gaaggtttcg attatatgga ccgcaaattg ttgctggcgg taatcgataa gttctttggt
     1681 gggccggtag gtctggataa cctggcggca gccattggcg aagaacgtga aaccattgag
     1741 gatgtgctgg agccttatct gattcagcaa ggctttttgc agcgtacacc gcgtgggcgt
     1801 atggcgacga cgcgggcgtg gaatcacttt ggcataacgc cgccagaaat gccgtaagtc
     1861 ggattggtcg ctcgcaccgc atccgacatt ggcagtgtgc ctgatgcgac gctattcgcg
     1921 tcttatccgg cctaccagag atagcgaacc gtaggtcgga taaggcgctc gcgccgcatc
     1981 cgacaaatgt gttcagcgac agattagctg gcctgctttt tcatcatact gataataaat
     2041 aacagtgcag cacatagcac taccgacgga cccgccggcg tatcgtaaaa cgcggaaaag
     2101 gttaaaccgc cagttactgc caccatcccc accaaaacag cgacaccagc catctgttcc
     2161 ggcgtacggg caaagcgacg cgcagtagct gcaggaataa tcagcaacga agtaataatc
     2221 aacgcaccga caaatttcat cgctacacca atcgtcaatg ccgtcaccag catcaacaac
     2281 aatttcacgc gctgtaattt cacaccatca acaaacgcca gatccgggct aatcgtcatc
     2341 gacagcaaat tgcgccattg ccagaacaaa atagccacca cgatgaccac gccaatcgca
     2401 atagagatga gatcttctgg cgtcactgcc agcaaatcac cgaacaggta agccatcaaa
     2461 tcaacacgaa tattagacat cagactaacg accaccaggc ccagcgacag ggcactgtgc
     2521 gccataatcc ctaataacgt gtcgatcgcc agctgtggac gcttctccag ccataccaga
     2581 ccgcccgcca gcagcagcgt aacggcaatc accgcataga atggattcac gtccagcaac
     2641 aaaccaaacg cgacaccaag taatgaggca tgagccagcg tatcaccgaa ataagacata
     2701 cgacgccaga ctacaaacga acccagcgga cccgcggcac aggcgagcat gatcccggct
     2761 aaccaaccgg gaaataataa ttcaatcatg agcgatcatt tccccgacgc aaaacaattc
     2821 gtccctgtaa atcgtgacga tggttatgat gatggcgata gatacccagt tgttcagcac
     2881 cacgaggacc aaacattgaa ataaactccg gatgcaggga aacaacttcc ggtgtgccgg
     2941 aacaacaaat gtggtgattc aggcacagca cttcatcggt ttttgccatc accagatgca
     3001 gatcgtgaga aaccattaaa acgccacaat ccagttcacg acgcagttgg tcaataaggt
     3061 catataacgc cacctggcca tttacatcca cgccttgagt gggttcatcc agcactaata
     3121 attgcgggcg atttaacaat gctcgcgcta acagtacacg ctgcgtttcg ccaccagaga
     3181 gcttttgcat cggtgcgtta atcaggtgcc cggcctggac acgtttcagt gcaggcaaaa
     3241 tatcttcttt atgtgtacca gggcgtaagc gtaaaaaacg gtttacggtc agtggcaacg
     3301 tggtgtcgag atacagcttc tgcggtacat agccgatgcg cagttttccg ttgcgcttga
     3361 taaccccttc atcgggtgtt accagcccga gcactacccg taccagtgtc gacttacctg
     3421 cgccatttgg cccaagtaaa gtcaaaattt ttccaggttt aagttccagc gacacatcag
     3481 agaggacgcg gcgttggcca aaagaaaccg agacattttc caaggaaacc agacttgtca
     3541 tgttaatttt agtcttgcag tagtcatgaa atgttataat atcacacttc tcatattcat
     3601 tacgatgatt ggtcgcatta tgttacataa aaaaacgctt cttttcgcag cattatccgc
     3661 cgctctctgg ggaggtgcaa cacaggccgc agatgccgcc gttgtcgctt cgcttaaacc
     3721 cgttgggttc atcgcttctg ccattgctga tggggtaaca gaaacagagg ttttacttcc
     3781 tgacggcgct tcagaacatg attattcact gcgcccatcg gatgtaaaac gcttacagaa
     3841 cgcggactta gtcgtttggg ttggcccgga gatggaagcg tttatgcaaa aaccggtaag
     3901 caaattacca ggagcgaagc aggtaacgat tgcgcagctt gaagatgtga aaccgctact
     3961 gatgaaaagt attcacggcg atgatgatga tcacgaccac gcggaaaaaa gtgacgaaga
     4021 tcaccatcac ggcgatttca acatgcatct ttggctttcc ccagagatag cgcgggctac
     4081 agcggttgca atccatggaa aattagtgga acttatgccg caaagtcgag ccaaacttga
     4141 cgccaacctg aaggattttg aggcacaatt agcctcaacc gaaacgcagg ttggtaacga
     4201 gctcgcgccg ctcaagggga aaggttattt cgtttttcac gatgcttacg gctatttcga
     4261 aaaacagttc ggactgacac cgcttggtca ttttaccgtt aaccctgaga ttcaacctgg
     4321 cgcgcagcgt ttacatgaaa taagaacaca gttggttgag caaaaagcaa cctgcgtttt
     4381 tgctgagcca cagttcaggc cagcggtcgt tgagagcgtc gcacgaggga catccgttcg
     4441 tatgggaacg ttggatcccc tggggacgaa tatcaaactg ggtaaaacaa gctattcaga
     4501 attcctgagt caattagcca accagtatgc gagctgcctg aaaggagatt aatgaggaag
     4561 tgattacgtg caacagatag cccgctctgt cgccctggcg tttaataatt taccgcgacc
     4621 acaccgcgtt atgttggggt cgctcaccgt tcttactctg gccgtcgctg tctggcggcc
     4681 ctatgtttat caccgcgatg ccacgccaat tgtcaaaacc attgagctgg aacagaacga
     4741 aattcgttcg ctcttacctg aagccagtga gccgattgat caagctgcac aagaagatga
     4801 agccattccc caggacgaac tggatgacaa aatcgccggt gaagcgggcg tgcatgaata
     4861 tgttgtttcc actggcgata cgctaagcag cattctcaat cagtatggta ttgatatggg
     4921 tgatatcacc caactggctg cggccgacaa agaattgcgt aacctgaaaa tcggtcaaca
     4981 actctcctgg acattaaccg cggacggcga actgcagcgc ctcacctggg aagtgtctcg
     5041 tcgtgaaacc cgaacctatg accgtactgc cgctaacggt tttaaaatga ccagcgaaat
     5101 gcagcaagga gagtgggtta acaatctgct gaaaggtacc gtcgggggga gctttgttgc
     5161 cagcgccaga aacgccggtt taaccagcgc cgaagtgagc gcagtgatta aagccatgca
     5221 gtggcaaatg gatttccgca aactgaaaaa aggcgatgaa tttgcggtgt taatgtctcg
     5281 agaaatgctt gatggtaaac gtgagcaaag ccagctgctg ggcgtacgtt tgcgttcaga
     5341 aggtaaagat tattacgcta tccgcgctga agatggcaaa ttctacgatc gtaacggtac
     5401 tggtctggcg aaaggattct tgcgattccc gacggcgaaa cagttccgta tctcgtctaa
     5461 ctttaacccg cgtcgtacta atccggtgac cggtcgcgtt gcagcacaca gaggtgttga
     5521 tttcgccatg ccgcagggta cgccagtgct ttcagtgggt gacggtgaag tggtggttgc
     5581 caaacgcagt ggcgcagcag gttattatgt ggctattcgt catggtcgca gctacaccac
     5641 gcgttatatg cacttgcgca agattctggt gaaaccggga cagaaggtga aacgtggcga
     5701 ccgtatcgcg ctttccggta ataccggacg ttcaaccggg ccgcatctgc actatgaagt
     5761 atggataaac cagcaggccg taaacccgct gacggcaaaa ctgccgcgta ccgaagggct
     5821 gaccggctcc gatcgtcgcg aattcctggc gcaggccaaa gagattgtgc cgcagctacg
     5881 gtttgattaa ttaacatcca ttcgcagccg gtacgcagtc agtatcggct ttttttattt
     5941 ggtgcggggc aagttgcgcc gctacactat caccagattg atttttgcct tatccgaaac
     6001 tggaaaagca tggaaacgaa aaaaaataat agcgaataca ttcctgagtt tgataaatcc
     6061 tttcgccacc cgcgctactg gggagcatgg ctgggtgtag cagcgatggc gggtatcgct
     6121 ttaacgccgc caaagttccg tgatcccatt ctggcacggc tgggacgttt tgccggacga
     6181 ctgggaaaaa gctcacgccg tcgtgcgtta atcaatctgt cgctctgctt tccagaacgt
     6241 agtgaagctg aacgcgaagc gattgttgat gagatgtttg ccaccgcgcc gcaagcgatg
     6301 gcaatgatgg ctgagttggc aatacgcgga ccggagaaaa ttcagccgcg cgttgactgg
     6361 caagggctgg agatcatcga agagatgagg cgtaataacg agaaagttat ctttctggtg
     6421 ccgcacggtt gggccgtcga tattcctgcc atgctgatgg cctcgcaagg gcagaaaatg
     6481 gcagcgatgt tccataatca gggcaacccg gtttttgatt atgtctggaa cacggtgcgt
     6541 cgtcgctttg gcggtcgtct gcatgcgaga aatgacggta ttaaaccatt catccagtcg
     6601 gtacgtcagg ggtactgggg atattattta cccgatcagg atcatggccc agagcacagc
     6661 gaatttgtgg atttctttgc cacctataaa gcgacgttgc ccgcgattgg tcgtttgatg
     6721 aaagtgtgcc gtgcgcgcgt tgttccgctg tttccgattt atgatggcaa gacgcaccgt
     6781 ctgacgattc aggtgcgccc accgatggat gatctgttag aggcggatga tcatacgatt
     6841 gcgcggcgga tgaatgaaga agtcgagatt tttgttggtc cgcgaccaga acaatacacc
     6901 tggatattaa aattgctgaa aactcgcaaa ccgggcgaaa tccaaccgta taagcgtaaa
     6961 gatctttatc ctatcaaata aaaaaagcct ctcaaattat tgagaggctg tactcgataa
     7021 aaaatctcaa tgagtgttcc cactattttg agtttttagg ttagtacgct attgagttaa
     7081 tcgtagcgtg ataatttcaa aaggtttgaa ctcaagattg caatattgat tgcaataaaa
     7141 actgtcatct aacttatttt cttgaagatc gacatattga acgtagtgat aattacatac
     7201 tcacgaaagc atggctgaaa tttctaatcc atgactctca tataatctta cgattatacc
     7261 gtcgccgtct tcagctaatt ttattgtttc gatgataacg ctgttagtat cgatagcaaa
     7321 aaatgatagt gaagggggta attcacccgg gtgcggtttg acagggacga cctgtaaggg
     7381 atgattcatt atataggctt gattaataac accacccaca cggtaatctc catgatgagg
     7441 aaataaggag aatatgaatt tttgtttacc aatatctgca aaattattaa tcaggaaatt
     7501 atcataattg tctgtcttaa caacttcccc aggtttatgc tgcgaacgca gcaaagttaa
     7561 ttcaagcgtc tggtctttga cacgatagcc atatttgttt tttgctatta aagctatgcc
     7621 agtttctcga tcagaaaggt cgacccagtg atgtgctggt atttcatctt tggcaaaagc
     7681 gaacgaatca tcgctatgcg ttggtctgtc aattacaccg aactgaattt gacattgtgc
     7741 aagagatgtt tgtactgaca ccgggaattt tactttgaga gaaacattag gttcgtgcca
     7801 atcaataaac gtttggacat caacgcgtgg actatcctgt gtcagggtaa aggctaaacg
     7861 aagataagag gaattgtagt gataaatcag cgtcatcgta gttttaggcc cgctgatcac
     7921 agttgtcatt ttttcagcga gtaaaacata tgattcccca tcacgataat ttgaagcaaa
     7981 atcccaggcc gcgaaaaaac ctgcatcttc gtggtatgca cggatctcac tgtgcat
//
LOCUS       DAZOYS010000177         7940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOYS010000177 DAZOYS010000000
VERSION     DAZOYS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.178"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(117..380)
                     /gene="cedA"
                     /locus_tag="ACHUN9_003423"
     CDS             complement(117..380)
                     /gene="cedA"
                     /locus_tag="ACHUN9_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0696736.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            563..2824
                     /gene="katE"
                     /locus_tag="ACHUN9_003424"
     CDS             563..2824
                     /gene="katE"
                     /locus_tag="ACHUN9_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0696737.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3082..3840)
                     /gene="chbG"
                     /locus_tag="ACHUN9_003425"
     CDS             complement(3082..3840)
                     /gene="chbG"
                     /locus_tag="ACHUN9_003425"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0696738.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3853..5205)
                     /gene="celF"
                     /locus_tag="ACHUN9_003426"
                     /gene_synonym="chbF"
     CDS             complement(3853..5205)
                     /gene="celF"
                     /locus_tag="ACHUN9_003426"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0696739.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5310..6152)
                     /gene="chbR"
                     /locus_tag="ACHUN9_003427"
     CDS             complement(5310..6152)
                     /gene="chbR"
                     /locus_tag="ACHUN9_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0696740.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6160..6510)
                     /gene="chbA"
                     /locus_tag="ACHUN9_003428"
     CDS             complement(6160..6510)
                     /gene="chbA"
                     /locus_tag="ACHUN9_003428"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0696741.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6561..7919)
                     /gene="chbC"
                     /locus_tag="ACHUN9_003429"
     CDS             complement(6561..7919)
                     /gene="chbC"
                     /locus_tag="ACHUN9_003429"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0696742.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
BASE COUNT         2130 a         2081 c         1779 g         1950 t
ORIGIN      
        1 atagcaatag ctcgtcaaag gcagcttttt gttacaggtg gtttgaatga atgtagcaac
       61 gaaatgcaga atttcaggtc atgtaactcc cggcaaaacc gggaagtatg taatccttac
      121 tcagtcactt ccccttcctg gcggatctga tttgcccaac gttgggcaga ttcaggcaca
      181 gtaaacgccg gtgagcgcag aaatgactct cccatcagta caaacgcaac atatttgcca
      241 cgcagcatcc agacatcacg aaacgaatcc atctttatcg catgttctgg cggcgcgggt
      301 tccgtgcgtg ggacatagct aataatctgg cggttttgct ggcggagcgg tttcttcatt
      361 actggcttca ctaaacgcat attaaaaatt agaaaaactg tagtttagcc gatttagccc
      421 ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtc tccgaagcgg
      481 gatctggctg gtggtctata gttagagagt tttttgacca aaacagcggc cctttcagta
      541 ataaattaag gagacgagtt caatgtcgca acataacgaa aagaacctac atcagcacca
      601 gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgagga
      661 cggatctcat cgtccagcgg ctgaaccaac accgccaggt gcacaaccta ccgcccctgg
      721 gagcctgaaa gcccctgata cacgtaacga aaaacttaat tctctggaag acgtacgcaa
      781 aggcagtgaa aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg atgatcaaaa
      841 ctcactgcgt gccggtaacc gtggtccaac gctgctggaa gattttattc tgcgcgagaa
      901 aatcacccac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc
      961 agctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct
     1021 ctcagatccg aacaaaatca ccccagtatt tgttaggttc tctaccgttc agggtggcgc
     1081 tggctctgca gataccgtgc gtgatatccg tggttttgcc accaagttct atactgaaga
     1141 gggtattttt gacctcgttg gcaataacac gccaatcttc tttatccagg atgcgcataa
     1201 attccccgat tttgttcatg cggtaaaacc agaaccgcac tgggcaattc cacaagggca
     1261 aagtgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt
     1321 gatgtgggcg atgtcggatc gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg
     1381 tattcacacc ttccgcctga ttaatgccga agggaaggca acgtttgtac gtttccactg
     1441 gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac tcaccggacg
     1501 tgacccggac ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg attttccgga
     1561 atacgaactg ggcttccagt tgattcctga agaagatgaa ttcaagttcg acttcgatct
     1621 tctcgatcca accaaactga tcccggaaga actggtgccc gttcagcgtg tcggcaaaat
     1681 ggtgctcaat cgcaatccgg ataacttctt tgccgaaaac gaacaagcag cattccatcc
     1741 aggtcatatt gttcctggtc tggacttcac caacgatccg ctgttgcagg gacgtttgtt
     1801 ctcctatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg agattccgat
     1861 taaccgtccg acctgccctt accacaactt ccagcgtgat ggcatgcatc gtatggggat
     1921 cgacactaac ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac
     1981 accgccggga ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg aaggaaataa
     2041 agttcgtgag cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct
     2101 aagtcagacg ccattcgagc agcgccatat tgtcgatggt ttcagttttg agttaagcaa
     2161 agtcgttcgt ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac
     2221 tctggcccag gcggtggcga aaaatctcgg tatcgaactg actgacgacc agctgaatat
     2281 caccccacct ccggacgtca acggtctgaa aaaggatcca tccttaagtt tgtacgccat
     2341 tcctgacggt gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg aagtgagatc
     2401 ggcagacctt ctggccattc tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct
     2461 ctactcccga atgggtgaag tgactgcgga tgacggtacg gtgttgccta tagccgctac
     2521 ctttgccggt gcaccttcgc tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc
     2581 ggatatcgct gacaacggcg atgccaacta ctacctgatg gaagcctaca aacaccttaa
     2641 accgattgcg ctggcgggag acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca
     2701 gggtgaagaa gggattgtgg aagctgacag cgctgacggt agttttatgg atgaactgct
     2761 aacgctgatg gcagcacacc gcgtgtggtc acgcattcct aagattgaca aaattcctgc
     2821 ctgatgggag gcggcgcaat tgcgccgcct caaatgattt acatagtgcg ctttgtttat
     2881 gccggatgcg gcgtgaacgc cttatccggc ctacaaaact gtgcaaattc aatatattgc
     2941 aggaaacacg taggcctgat aagcgaagcg catcaggcag ttttgcgttt gtcagcagtc
     3001 tcaagcggcg cagttacgcc gcctttgtag gaattaatcg ccggatgcaa ggttcacgcc
     3061 gcatctggca aacatcctca cttacacatc ccgataactc cccaaccgat aaccacgctg
     3121 agcgatagca cctttcaacg acgctgatgt caacacatcc agctccgtta agcgtgggaa
     3181 acagtaagca ctctgacgga tagtattatc gataaacgcc ggatgacaca tcacctccag
     3241 cgaacgatca ccccgatggc ttgcgtcatc cagcacttgc aggaacagtg cctcgctaat
     3301 ctcttcacca taaaacgcgc tactgaatcc ctgagaactg cgcagattag ccggtaaatc
     3361 accagagtta gatagcggct gacggtcgat gcgcaacgca atcccctctt cagccgcaaa
     3421 cctggcaaca atcgggaaaa tctgcgggaa catatgcaca tgatgatggc tatcaagatg
     3481 cgtaggtttg cgtccaaata gctcaatgaa acgcaaatac tgactggcaa gctcctgagt
     3541 aatttcttcc agcggtaaag catcttcttc tgccaactgc cagatccatt ttcccagcac
     3601 accatcgcgg gttaaccccg gcatagctgt cagtggcttg cccatagtaa ggacaaagta
     3661 catccctatg gccagactcg gttcatcacg actcaactgc accgcatggt caatagcctg
     3721 cccattcacc agcgccgtcg tcgatgtgac aatcccattg cgacaggcct cgataatgcc
     3781 gtagttctga cctttgctta agccaaaatc atcggcatta acaatcagta agcgttccat
     3841 aatcagcctc ggttaatgtg cttttttaag ctctgcgatg cagtcggcaa aatttggcag
     3901 ccatttctcg tgcgccagaa tcatctcgcg tgccagcagc tcagcatcgc gatcggaatg
     3961 caccaacgga ctaaggttta gcgccagtaa cacatcgtta aattctccgc taagtgcggc
     4021 gttgctggca gcaatctcga agcctttaat ggtgtgaatc agccccatca ctttatcatc
     4081 gaaatgcgta atgcgcggat gtggcgtcgc gccatcgcgc cccagcttac aggtcatttc
     4141 taccgcccag tctgccggaa tattatcaat atgcccatga tgcgggatat taacgtaatg
     4201 ttcagcttgc ttgtcgttgt agatagcgtt gatcacttcg cacgctgcat cagagtaata
     4261 agccccaccg cgctgttcca gttctttcgg cttaactttc aactccgggt ttttatacag
     4321 ctcaaaaagt tgtttctcga ctttctgtac tacctgcgct cgtgcgccgc ctttgtagta
     4381 ttcgcccatt tcaatagcca gcatctcttt ttgcttgaag taataaagca aataagaaca
     4441 cggcaacaga ttcagagaac gaattaagcc ttcgctaaat ggcagatcga aaatattttt
     4501 aacgccagat gcttttaact gcccggatgc cacaccatca agcaattcgg caaagcgcga
     4561 cttgccattt accagcacat ccttaatgaa caccatatgg ttgaggccga acaaatcgat
     4621 agataaatca tcgctgtctt tcagcatcag aacatcgtga ataaacatct tcatgccgat
     4681 cggaatatta cacacgccga taaagcgttt aaatccggta tgacgataaa cggcttcagt
     4741 aaccattccc gccgggttag tgaagttaat cacccatgca ttcggacaaa gttcttcgac
     4801 atcttttacg atgtcaaaaa tcaccggaat ggtacgcaga cctttaaaca gaccgcccgc
     4861 gccgttggtt tcctggccaa gataaccatg acttaatgga atacgttcat ccagttcgcg
     4921 cgccggtaat tggcctacgc gcagttgggt agtaacgaaa tcagcatctt tcaatgcttc
     4981 gcggcgatcc agcgttttat aaagcttcat cgggacgcca gcgttatcaa tcatccgttg
     5041 gcagagttca aaaatgatat ccagtttctc tttaccatct tcgacatcca ccagccataa
     5101 ttcgctgacc ggcaattcgt gataacgctt aataaatcct tccagtaact ccggggtata
     5161 gctgctcccg ccaccaatag tgacgacttt taatttctgg ctcataattt ctcccttcag
     5221 tacagaatac tgatatctgg catatctgcc cccccggaca taaataatcc agcaacagga
     5281 cagatatgtg aattgtcagg tataacgact tactgattaa attcagtcaa tttcttacga
     5341 tagctcttag gcgtaaagga cgttaatttc ttaaacgttt taataaacaa actcgggcta
     5401 ctataaccgg cctcaaacgc aatatccgtc acggaatagt tggtcatttc cagttgtttt
     5461 ttggcaaaat taatacggat ctcattaata atctgcattg gtgttttgcc ataatatcgt
     5521 tgagtcgctc gcgtcaaata ttcctgtgat ttggctgaca acgtcaccat attctccagc
     5581 gccgattcac taaactgctc tttatcatgc atcttttcta ccgtactttt cagccactgc
     5641 ggtacatcat caatcacctg ttcttcgcgg taatgacgta aacggttaat gacataaaaa
     5701 gtaaccatct caacaaactc ttccagccct gtttcgcgga aattcaatga agaaatcact
     5761 gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc gacaaagcaa
     5821 taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc aacgttcaat
     5881 atacgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg aataaaaaca
     5941 aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctggaaata acgcccggtt
     6001 aataccagag taaattcata atagtcgtgc tgatgcagcc cgctgatact ctcagttttg
     6061 ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg ggcagtggca
     6121 atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca gtttttcatg
     6181 aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta aatgatcctg
     6241 agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc cttcaatcag
     6301 tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca tcatggcttt
     6361 tgctgcagca aaatcgccct gcttcgcctg tttcagtgcc gcatacgcca ggctgcgcgc
     6421 ttgtccggag ttgatgataa gccccatcac cacttcttcc agctcttcag cttccgtttg
     6481 cgtatcggga atgttatcga gatccatcat acatcgttcc tctctattct taccggcacg
     6541 attacccgta ccggcatcga ttaaaatttc agggcgttag cgatatcttc ttcgcgctct
     6601 tctttatcaa tcgcgttttg tgctttgtta gccaccacaa caaagggcag ataaattaac
     6661 gttgcgatac caaggttgaa gagtgcaacc agcaatgcgg cgacgctacc gttggtgtta
     6721 aagaaggctc ccagaccggt tggcatggtc cacggtgcaa tattggtcac cggaggaatg
     6781 atgcccatgt agtacgctgc gagggtgatt gccgccagaa tcggttgtac cagtacaaac
     6841 gggataaaca tcaccgggtt catgataatt ggcagaccaa acagaatcgg ttcgttaatc
     6901 tggaagatgc cggacggcag cgccagtttt gccacctgac gataatcagc acggcgagag
     6961 gcgataaaga tagccaggat caggcctaaa gtcgcaccac tgcccccaag gaaaataaag
     7021 gagtccagca tcggcttggc ccagatatgg aaggtcttac cggctgccag cgccgcttcg
     7081 acggaaccat attgctgata ggtcgcgata ttttccagcg cccacggcgt cataatgccg
     7141 ttgtccagtg cggtcagcgc cagcgcgcca tgaataccga agaaccagag cagtggaaca
     7201 aagatcacat aggcccagcc caccacgcta cccaacgatg ccagtggggt tgagatggta
     7261 tccataatga tctgatggaa gttggtgccc caggtattca acgcccaggc aataatcccc
     7321 atcacggaaa gaataataaa gccgggaatt aatgccgaga aggaacgcga tactgaagca
     7381 ggtacgctat cgggcaattt aatgacccaa ttgcggcgga caataaaggt aaacatttct
     7441 gccaccacca ggccaataat aatcccggag atgatattcg ctccacctaa ccagtttgcg
     7501 ccaaccacat aggcctcacc gacactatat ggggtgacgg tcataaatgc tgcaacggat
     7561 aacaacccag ccgccagcgc atcgacttta cgctcttctg ccagcgccat gccaataaag
     7621 aacggtgcca ttaaagacat tatccctaat gttccgttat atacgttgcc gccaatacct
     7681 ttcaggccat taagtgtttc aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa
     7741 aacgccccct ccccaaagct tagaaaaacg ttgttaatta atacaaacat ggcccccgca
     7801 agggttaacg gcattaagcg aataaagcca tttttgattg cattaacgtg tggctgcttt
     7861 cctattttaa ctgcaaaagg gaggagtacc ttttcaagcg atgcaataac attactcata
     7921 gaaaaatacc cttaaaaacc
//
LOCUS       DAZOYS010000178         7923 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOYS010000178 DAZOYS010000000
VERSION     DAZOYS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.179"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHUN9_003430"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHUN9_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0696743.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHUN9_003431"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHUN9_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0696744.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUN9_003432"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUN9_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0696745.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUN9_003433"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUN9_003433"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0696746.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUN9_003434"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUN9_003434"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0696747.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUN9_003435"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUN9_003435"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0696748.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUN9_003436"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUN9_003436"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0696749.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHUN9_003437"
     CDS             5315..6439
                     /locus_tag="ACHUN9_003437"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0696750.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUN9_003438"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUN9_003438"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0696751.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>7923
                     /locus_tag="ACHUN9_003439"
     CDS             7245..>7923
                     /locus_tag="ACHUN9_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0696752.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNL"
BASE COUNT         2014 a         1833 c         2027 g         2049 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 tta
//
LOCUS       DAZOYS010000179         7804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOYS010000179 DAZOYS010000000
VERSION     DAZOYS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.180"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUN9_003440"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUN9_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0696753.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUN9_003441"
     tRNA            1230..1305
                     /locus_tag="ACHUN9_003441"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUN9_003442"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUN9_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0696754.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUN9_003443"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUN9_003443"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0696755.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUN9_003444"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUN9_003444"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0696756.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4735)
                     /gene="cobU"
                     /locus_tag="ACHUN9_003445"
                     /pseudo
     CDS             complement(4189..4735)
                     /gene="cobU"
                     /locus_tag="ACHUN9_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            complement(4869..5306)
                     /locus_tag="ACHUN9_003446"
     CDS             complement(4869..5306)
                     /locus_tag="ACHUN9_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0696757.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHUN9_003447"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHUN9_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHUN9_003448"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="ACHUN9_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0696758.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2184 a         2074 c         1663 g         1883 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgcccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaa
//
LOCUS       DAZOYS010000180         7777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOYS010000180 DAZOYS010000000
VERSION     DAZOYS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.181"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUN9_003449"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUN9_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0696759.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUN9_003450"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUN9_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0696760.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUN9_003451"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUN9_003451"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0696761.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUN9_003452"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUN9_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0696762.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUN9_003453"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUN9_003453"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0696763.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUN9_003454"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUN9_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0696764.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="ACHUN9_003455"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="ACHUN9_003455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0696765.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2103 c         1872 g         1825 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAZOYS010000181         7728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOYS010000181 DAZOYS010000000
VERSION     DAZOYS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.182"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(195..746)
                     /locus_tag="ACHUN9_003456"
     CDS             complement(195..746)
                     /locus_tag="ACHUN9_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0696766.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1333..1680
                     /locus_tag="ACHUN9_003457"
                     /pseudo
     CDS             1333..1680
                     /locus_tag="ACHUN9_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1733..2128
                     /locus_tag="ACHUN9_003458"
     CDS             1733..2128
                     /locus_tag="ACHUN9_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0696767.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2169..2912
                     /gene="cmoA"
                     /locus_tag="ACHUN9_003459"
     CDS             2169..2912
                     /gene="cmoA"
                     /locus_tag="ACHUN9_003459"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0696768.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2909..3880
                     /gene="cmoB"
                     /locus_tag="ACHUN9_003460"
     CDS             2909..3880
                     /gene="cmoB"
                     /locus_tag="ACHUN9_003460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0696769.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4045..6474)
                     /gene="torZ"
                     /locus_tag="ACHUN9_003461"
     CDS             complement(4045..6474)
                     /gene="torZ"
                     /locus_tag="ACHUN9_003461"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0696770.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6499..7599)
                     /locus_tag="ACHUN9_003462"
     CDS             complement(6499..7599)
                     /locus_tag="ACHUN9_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0696771.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1844 a         1972 c         1753 g         2159 t
ORIGIN      
        1 tctgaaaatt tttataaaaa atgactagcc ctctttgaat ctcatcttcc ccgaatcttc
       61 gaaaatatct gtctagttga ttcggtagaa tctacctcat gatgtatacg tgtttagtac
      121 ccgtcacacc agctatgtga cccccttggc aaaaaagcca aatatcagct acatccatgt
      181 aactattcag taacttattt agcaagtctg taataagttg ctctagagat tcctagctct
      241 tcctgaatct gcaacctcgt tttaccttgt gaaataagca tctgtacagc ctctgaagaa
      301 gcttttacag gtcttcccat atgtacccct tcagctttag cacgttcaat accagccttt
      361 ctacgctctg ctatcaagct tctttcaagc tgtgccaacg ttgacataat gcctaacatt
      421 gcttcaccca tagcgcttga taagtcaaat ccttctctaa ggctgataac tgtcacacct
      481 ttagcctgta gcgctttaac cgttgataac acatcaattg tgtcacgtcc aagacggtct
      541 acagctacca caactacggt gtcaccttct cgtgcataag ctaacaaact gctgaaaccg
      601 tttcttaaag aagctttgac agaaccactg acagcttcat cagcgaacca tttagcaatg
      661 ttgtaccctg cctcttcaat ttgcatttgc tggttagtga tggattggtc atctgtactc
      721 accctgcaat atccaagtat cgccataatg ttccccttag aagtctcaaa agttagtctc
      781 ataagtttag ctgtctcata atccaaaaac aaccttttga gactacttga gggtgcattc
      841 taccgttacg ttgaaagtgt ctcaaaagga cttccttatg agactggtta atagagcgct
      901 atgaagattc aggagggtaa ctataaccat cactttctaa ctttcacgta ggaaatcgtt
      961 aagtttcttg atagttacac catgtgacca actatctata tatcgttata tagtcctgta
     1021 tatagcacta tatgtagtgt ctggagaggg acgactaaca cgatacagtt taccctgaaa
     1081 agagacttca cagattcttt atatatttca ataagttatg ctaaaaagag actataccga
     1141 tactgcatag aatttgtgta ggattgcata gatacttgga aaaacaccgt taaattgcat
     1201 agtcattcac ctgatattgt cctgttttta caggtagttg attcggatgt tctaggaatc
     1261 atcctcatag ttgattcggt tagttgggct tgtaccaacc cctaatcaac ctcatagtgt
     1321 atttagacct tgatgatttt agacagccgc cctgttcatg cagcacgtcc acacagtgaa
     1381 gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt actgacggcg
     1441 acaaatccgc tgattcgttt tatcggtagt gatgatatga cgcaaaaccg ggaattattt
     1501 caggtctggt tacagaaatt agcgcagtgg catcagacca ctacgcctta tcttttttta
     1561 catacgccag acattgccca ggccccggaa ctggtacata ccctgtggga agacttacgt
     1621 aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc tcttttctga
     1681 atttgccacc tatcatagac aggtgccatc ggccatttta aagggagttt gtatggtaag
     1741 cgcgctatat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg atgtcgttcg
     1801 cctgcgaatg cagtaccgcg ttgcctatgg tgacggtggt tttagcgaac tgcaaagcgc
     1861 tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgc tgatgctgtt
     1921 tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg ttttgcttgc
     1981 tggtcgtctg atgcattatt acggttttca tcaccgtctg tttcgctggc gacgttccgg
     2041 catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt ggtatatgcc
     2101 ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt ttcccggatt
     2161 tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga ctgggcgact
     2221 ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt tccgttcccg
     2281 gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt caacctggta
     2341 cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg cgtcgcaaca
     2401 ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg attgaacgct
     2461 gcagtcgtca tattgacgcc tataaagccc ctacgccagt agacgtcatt gaaggtgata
     2521 ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc ctgcaattcc
     2581 tggaaccttc cgagcgccag gcattactgg ataaaattta tcaaggactg aacccgggcg
     2641 gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt gaactgttgt
     2701 tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag atcagccaga
     2761 agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat aaagcacgcc
     2821 tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac tttggttcac
     2881 tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt attctctgat
     2941 tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg ctaactggca
     3001 gcgcgagcag cagcacgggc tgtttaagca gtggtccaac gcggtggaat ttctgcctga
     3061 aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg aagagccact
     3121 gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc catggcgcaa
     3181 agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg actggaaatg
     3241 ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg atgtcggctg
     3301 cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg cggtggggat
     3361 cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac tgggtaacga
     3421 tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga aagcctttga
     3481 taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc atctctggca
     3541 gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg ttattgatgg
     3601 cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta atgtctattt
     3661 catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg tagatatccg
     3721 cattgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat ggatggttac
     3781 cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg aaggttatcc
     3841 tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggta ttactgccgg
     3901 atgcggcgtg aatgccttat ccggcctaca aacccttgct aattcaatct attgcaggga
     3961 ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata acctcagcgc
     4021 ccgtttccgg gcgctattca cttcttacgc ctgaacagca ggcttatcaa atgccgttaa
     4081 cttcggtgcg ttacccgtat atttttcgat atacaccagc gcagagttac cggcacaggc
     4141 atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc cgtttttaca
     4201 caagccgttt tccagatctg gccatgcacc ttcatgcacg cataccacgc cttttttgat
     4261 cccgtcagtc accaccgcgc ctgtcaaaat ctgaccacgt ttgttccaga cacgcaccag
     4321 atcgccattc gcaataccaa atcgagcagc atcttcggtg tgaatagtga tcggttcacg
     4381 atcggcaacc gcatattttt tacgcagttc cgcatagtta agctgactgt gtaaacggtg
     4441 tgccggatgt gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct tccactcatc
     4501 aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt ccagcgtttt
     4561 ggagtaaatc tcaattttgc cacttggcgt acccagcgca tttttcaccg gatcggcgcg
     4621 gaaatcacca taacgaacgt actgttcgtt cttctcgctg cggcgcattt cgatcagttt
     4681 attttgctgc cagaaggcat taaacattgg catagtgacg cgttgcgcac gggccccttt
     4741 ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt cggtatagat
     4801 ctcttttccg ccaggtttga gtaattccgc aagatcggca aacacatcaa aatcgtttcg
     4861 cgcttcaaat tgcggagcaa cagcctgctt catcggcaca atatgctggt tgctgtaatc
     4921 accggtcatc gtcaagtcat tgcgctcaaa cgatgtggtg atcggtaata cgatatctgc
     4981 gtgtttagca gctgcggtcc agtagcattc agaaacgacg atcatctccg gtttctgcca
     5041 tgctttaatc agacgattag tatcctggtg atgggtaaag ttaccgccac ccgcccacca
     5101 gatcattttg atattcggat acgtttgctc tttaccgtta tgctgatatt tgccgccagg
     5161 attttccagc gcatcgacga tacgggcaac aggaatcgcc ctcattccgc catcatcagc
     5221 ggcctcgctg gcctgtccgg caatcgccgc agacatttcc ggcagcacgc cgccaacacg
     5281 tgtcgggttg ccaccattgg agtaatgata agagaaacca aaaccgccgc ccggtgtacc
     5341 aatctgccct aacatcgccg ccagcgtcac cagcatccag tgtttctgtt caccatattg
     5401 ctggcgctga attccccagc ctgccatcag cattgtgcga ttcgccgcca tcagttcagc
     5461 gagtttgact atctgcgctt ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt
     5521 cggcgtatta tcgctcttac ctctcagata ctcttcaaac tgcggatacc cggtagtgta
     5581 tttttcaagg aacactttat cgtgtttgcc ttgtgtcatc agggtatgcg caatccctaa
     5641 catcagtgcc acgtcggtgc ccatattcgg cgcgatccag gtggcattat cgccaaagaa
     5701 gtcgatggtt tcggagcgga taggatcaat ggcaatcact ggtttgccag attttttcag
     5761 ctgatggaag tattccagcc cttgctcatc ggtactgctc caggcaattt ttaaggtatt
     5821 cagcgggttc atcccccaca gcaccacaac ctggctgttt tccagaatca gcggccagga
     5881 ggtctgctgt tcatacacct ctacagaacc gaccacatgc ggcatgatca cctgtgccgc
     5941 cccggttgaa taatcgccgc tatgcccgga ataaccgccc gccaggttca tataacgttg
     6001 cagtaaggtt tgcgctttat gcaacacgcc agaagagcgc cagccgtatg atccggcaaa
     6061 aatagccgat ggtccgttag ctttgcgaat gcgatcgtgt tgctcatgaa tcagttttaa
     6121 tgcttgttcc cagctcacct gtacataggt atcttcgcca cgacctttcg tcggttgcag
     6181 tggattatcg agatagcttt ttctcaccat cggatgctga atacgcgtcg tggtgtgtac
     6241 ctgatccgcc gccgtagact gtaaggaatt cggtatggtt ttcgccagcg cgcctgttga
     6301 agaaacaatc ttgccgtcct tcacttctac gttcatcgct ccccaacgtc ccgcggtgag
     6361 gattttaccg tccttctctt ctgcccatgc ggggagcggt gctgccgatg tcaccaccag
     6421 cgctccagcg gcaataccgc tgtgtttaat aaattcacgt cttgttaatg tcataacttc
     6481 ctccctgatc aacgaggatc actgtttctc ggtaatatct ttggcgttgt actggaaata
     6541 ccgcgttaaa atgtccagtt cgttttcgct catgctggtt cgtgccccca ttcctttggc
     6601 aatggacggc cacgcattga cggtgtaatg gtcggcggca ataggggcat gacaaccagc
     6661 gcaataggta tcgtcaagtt tttcagcgta ttgccatagc ggtttacggt ccgctaatcc
     6721 gggatcggta agcgcaccct gtaaagacgc ctgacgccat tgattgccgt attcgtcagc
     6781 ctgccattcc ccgtttaccg tgagcgcctt gataccttct tcacttaatg tggctagcgc
     6841 cagccgttga cctgccgcca ggtagagcgt gttttcactg ccctgcattt gataaccctg
     6901 caacagaacg atcggctgtt tgccactggc atcaacgacg gtgagatcgg taccaggatt
     6961 cacggtagcc agctcgccta tgcgagaagt tttgaaagga taaatatgtg cgccattagt
     7021 cactgaagta gcggcctgac tttccagctc atgcgccgcg ttgtcatcca tttttatttc
     7081 tggcggaaaa tgggcaatgc ctttatgaca atcgatacag gtttcgccgc ccttttgtgc
     7141 tttgttatgc attttctgcg cagattcact ttgcgaggca atatccatgg catcaaaaga
     7201 atggcaacta cggcacgttg cagagtcagt tgcttttaat tctttccata ctgtttcggc
     7261 catttcctgg cgatgagttt cgaacttatc gtcactgtct attttgccgc taacaaattc
     7321 atgataaata tctttagatg cttttaattt agcaaataaa taatccatcc ctgactttgg
     7381 aatatggcaa tctgcacatt ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc
     7441 ctgatattcc tcaaaaggtt tactcatcga gtggcaggaa aggcaaaatg ctgtatccga
     7501 cgttttatgt aaggcttttt gcgccagcaa taacccgccg ccaccaacca caaccgctat
     7561 cagcaaaaac aataacccaa tgcgtttttt ccctcgcata gattcctcta aataaattcc
     7621 agaacaccga aatcattgta taattaaata cacaacgaat taaccgcttg cataactcgg
     7681 cacaacactg cctgaaataa tcgataaaga atatgattta ttacaatg
//
LOCUS       DAZOYS010000182         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOYS010000182 DAZOYS010000000
VERSION     DAZOYS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.183"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUN9_003463"
     CDS             <1..643
                     /locus_tag="ACHUN9_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0696772.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUN9_003464"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUN9_003464"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0696773.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUN9_003465"
     CDS             2474..2986
                     /locus_tag="ACHUN9_003465"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0696774.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUN9_003466"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUN9_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0696775.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUN9_003467"
     CDS             5579..5782
                     /locus_tag="ACHUN9_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0696776.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUN9_003468"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUN9_003468"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0696777.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHUN9_003469"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHUN9_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0696778.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1782 a         2103 c         2022 g         1808 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZOYS010000183         7676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOYS010000183 DAZOYS010000000
VERSION     DAZOYS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.184"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            129..1907
                     /locus_tag="ACHUN9_003470"
     CDS             129..1907
                     /locus_tag="ACHUN9_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0696779.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHUN9_003471"
     CDS             complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHUN9_003471"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0696780.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHUN9_003472"
     CDS             complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHUN9_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0696781.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHUN9_003473"
     CDS             complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHUN9_003473"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0696782.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6024..6779
                     /gene="actS"
                     /locus_tag="ACHUN9_003474"
     CDS             6024..6779
                     /gene="actS"
                     /locus_tag="ACHUN9_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0696783.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6858..6927
                     /locus_tag="ACHUN9_003475"
     tRNA            6858..6927
                     /locus_tag="ACHUN9_003475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6890..6892,aa:Gly,seq:ccc)
     gene            7120..>7676
                     /gene="ygeQ"
                     /locus_tag="ACHUN9_003476"
     CDS             7120..>7676
                     /gene="ygeQ"
                     /locus_tag="ACHUN9_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR0696784.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQ"
BASE COUNT         1972 a         1804 c         1939 g         1961 t
ORIGIN      
        1 gcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
       61 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
      121 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
      181 agcgttttgc cagaatgctt gccagcgttt tgcagtttga ggttgagatc gttgatgaaa
      241 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
      301 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
      361 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat
      421 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
      481 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
      541 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
      601 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
      661 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
      721 ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc
      781 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
      841 tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt
      901 tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg
      961 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
     1021 tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg
     1081 gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg
     1141 aaagcgaact gttcggttat gttaaaggcg catttactag tgcttctgcc aacggtaaaa
     1201 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
     1261 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg
     1321 gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat cagaatttgg
     1381 cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
     1441 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg gtgcattact
     1501 ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg
     1561 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
     1621 aatatctggt taacgtggtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
     1681 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc
     1741 tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
     1801 aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg
     1861 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
     1921 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt
     1981 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc
     2041 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
     2101 ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga
     2161 gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcctttcc ttcagcccag
     2221 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt acaggcacca
     2281 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
     2341 gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg
     2401 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
     2461 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
     2521 atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca
     2581 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg
     2641 cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg
     2701 cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
     2761 tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt tcgtcatgct
     2821 caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc
     2881 gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc
     2941 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccag
     3001 caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata ggagcttcta
     3061 ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc gccagcgtaa
     3121 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
     3181 ggtggagctg aatcagtacg tcagtgccat cggcgagcag tttggcctgc gggttgtcag
     3241 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
     3301 tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc
     3361 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg
     3421 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
     3481 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
     3541 tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat ctcttcaaat
     3601 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagtgga
     3661 ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagc
     3721 gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccgatgggtt agtggtggtt
     3781 ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc
     3841 gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct
     3901 tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt
     3961 ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt
     4021 gaggcaaatg cgccgggagc gaacggcgta acgtcggtat cttgagttga aataacgcga
     4081 acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca
     4141 ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga
     4201 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtata gctaaaacag
     4261 gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc acggcgtttt
     4321 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
     4381 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
     4441 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
     4501 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
     4561 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt cctccccgca
     4621 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
     4681 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt aaaagcgtgg
     4741 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
     4801 ccaagcttgc tggtcaggaa tgctgccatt ggctcttcca gtacatcctg tttattacca
     4861 aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc
     4921 tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
     4981 gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg
     5041 ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta
     5101 cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct
     5161 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
     5221 aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca
     5281 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
     5341 tcaagtgtcc aggcatgccc agctgtagcg aatggaatat caggcacatc ttcccaggta
     5401 aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact tacggcataa
     5461 ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg
     5521 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca
     5581 cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct
     5641 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag
     5701 gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg
     5761 aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattggga
     5821 ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata cctctacagc
     5881 gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc
     5941 acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga taaggcgtct
     6001 tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta
     6061 tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg
     6121 atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta
     6181 tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc
     6241 cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta
     6301 cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg
     6361 taccgaaatc atcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga
     6421 aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc
     6481 cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc
     6541 tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg
     6601 cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg
     6661 ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg
     6721 caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat
     6781 ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat
     6841 gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta
     6901 tacgagggtt cgattccctt cgccgctttt gctgtatctg attttctata ttatcttaat
     6961 tcaatcgctt cagacttttc gcaatcgccg taatatttcc ccattttgtt gtaaataaaa
     7021 agcgtttacg caatgcaatc cttttgtaac aacccaggta aaacacctta ttttcaagtt
     7081 gttaaataag tggttattta cgcttttaac aaacgattga tggaatattt tatgaaaggg
     7141 aaatctgcat taactcttct gctcgctggt atattttcgt gcggcacatg ccaggcgaca
     7201 ggggcagaag tgacgagtga atctgtattt aacattctta attccacggg ggcagcaaca
     7261 gataaaagtt atttatcgtt aaatccagat aagtacccta attatcgtct tctgattcac
     7321 tcagccaaat taaaaaatga aataaagagc cactatacca aagacgaaat tcaagggttg
     7381 ttaacattaa cagaaaacac acgaaagtta acgttgacgg agaaaccctg gggaacattt
     7441 attcttgctt ctactttcga agatgacaaa acagcggcag aaacccacta tgatgctgtc
     7501 tggctgaggg atagtttatg gggctatatg gcattagtat cagatcaggg aaatagtgta
     7561 gccgcaaaaa aggttctgct caccctgtgg gattatatgt ctacgctcga ccagattaag
     7621 cggatgcagg acgtcattag caaccccaaa cgactggatg gagttcccgg gcaaat
//
LOCUS       DAZOYS010000184         7668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOYS010000184 DAZOYS010000000
VERSION     DAZOYS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.185"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            132..635
                     /gene="dps"
                     /locus_tag="ACHUN9_003477"
                     /gene_synonym="pexB"
     CDS             132..635
                     /gene="dps"
                     /locus_tag="ACHUN9_003477"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0696785.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1039..1785
                     /gene="glnH"
                     /locus_tag="ACHUN9_003478"
     CDS             1039..1785
                     /gene="glnH"
                     /locus_tag="ACHUN9_003478"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0696786.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1924..2583
                     /gene="glnP"
                     /locus_tag="ACHUN9_003479"
     CDS             1924..2583
                     /gene="glnP"
                     /locus_tag="ACHUN9_003479"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0696787.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2580..3302
                     /gene="glnQ"
                     /locus_tag="ACHUN9_003480"
     CDS             2580..3302
                     /gene="glnQ"
                     /locus_tag="ACHUN9_003480"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0696788.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3419..5644
                     /gene="ybiO"
                     /locus_tag="ACHUN9_003481"
     CDS             3419..5644
                     /gene="ybiO"
                     /locus_tag="ACHUN9_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0696789.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5641..6567)
                     /gene="rlmF"
                     /locus_tag="ACHUN9_003482"
     CDS             complement(5641..6567)
                     /gene="rlmF"
                     /locus_tag="ACHUN9_003482"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0696790.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6843..7103
                     /gene="mcbA"
                     /locus_tag="ACHUN9_003483"
     CDS             6843..7103
                     /gene="mcbA"
                     /locus_tag="ACHUN9_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0696791.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7368..>7668
                     /locus_tag="ACHUN9_003484"
     CDS             7368..>7668
                     /locus_tag="ACHUN9_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0696792.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1853 a         1963 c         1934 g         1918 t
ORIGIN      
        1 tattagtttt gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag
       61 cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag
      121 gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata
      181 cccgcaacga tgtctccgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg
      241 ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg
      301 ctaacttcat tgccgtacat gaaatgctgg atggcttccg caccgcactg atcgatcatc
      361 tggataccat ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag
      421 ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc
      481 acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg
      541 gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata
      601 aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt
      661 tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccacctcaa
      721 cgcacttatt tagtgcatcc atctgctatc tccagctgat taagtaaatt ttttgtatcc
      781 acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat
      841 cactgtgttg agtgcacaat tttagcgcac cagattggtg ccccagaatg gtgcatcttc
      901 agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaaggagt
      961 tgcaaaactg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga
     1021 tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg
     1081 gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc
     1141 ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg
     1201 gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg
     1261 atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc
     1321 gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg
     1381 atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt
     1441 gctgttaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat
     1501 ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac
     1561 gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag
     1621 ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtattgcgtt cccgaaaggt
     1681 agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact
     1741 tacaacgaaa tctacaaaaa atggttcggt actgaaccga aataataacg ctacacctgt
     1801 aaagcgcact ggcagttccc tctcccctct ggggagagga ttagggtgag gggcgcaaac
     1861 ccgctccggg gccattaatt accctgaatt tgattattta caccacggta acaggaacaa
     1921 catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc
     1981 aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat cggattgctg
     2041 gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt
     2101 gaggtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatttattt cgccctgccg
     2161 atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc
     2221 aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat tcacaaaggt
     2281 tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt
     2341 ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt
     2401 aaagacacct cgctgtttat tgtgatcggc gtggcggaac tgacccgtca ggggcaagaa
     2461 attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg
     2521 attattaccc tggtgctaag ctttattttg cgtcgtctgg aaagaaggat gaaaatcctg
     2581 tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctt cacaatatcg
     2641 atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat
     2701 cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg
     2761 atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta
     2821 tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg
     2881 gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc
     2941 tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc
     3001 aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg
     3061 atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg
     3121 atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga
     3181 aagtagcttc gcggctgatc tttatcgaca aaggccggat tgcggaagat ggcgatccgc
     3241 aggtattgat caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt
     3301 aacttctctg cctccctttc acccgaaagg gaggcacacc agattcctct catttaaaat
     3361 cgcccctccc ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat
     3421 gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat
     3481 acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga
     3541 tatcgaacaa aaaaaagcgg cctatggcgc actggcggat gtgctggata acgacacctc
     3601 gcgtaaagaa ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg cggaaccggt
     3661 cccgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga
     3721 agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat
     3781 caccggctcc ccgcataagc catttaatcc acaaaccttc agcaatgcgc tgagccattt
     3841 ttcaatgtta gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc
     3901 gctgtatcgc aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca gcaactggtt
     3961 gcagcttccg gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggccctgac
     4021 attatttgtc ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt
     4081 ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag ccgtactacg
     4141 cctgattttt tgcccaaacg tggcggagct gcgcccgttc acgattcagg acgagagcgc
     4201 ccgttactgg agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt
     4261 ggccgtgccg attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat
     4321 cattatgctg tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata aaaaagagat
     4381 tacccagcat ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg
     4441 cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt
     4501 ttcgttgttc gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct
     4561 ggcgattatt ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc tggcaaaaac
     4621 catcactctc tcgccacata ctcagcgtaa ctatccagag ttgcaaaaac ggctgaatgg
     4681 ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt
     4741 gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca
     4801 gaaaaccgta gatatcctga tccgtatcgc acttattctt tttttctcgg cggttggctg
     4861 gacggtgctc gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct
     4921 acccagcgcc cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg cggtgattat
     4981 cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct
     5041 ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga
     5101 tatcattacc ggggtgttta ttcagtttga aaacggcatg aacactggag atttggtgac
     5161 tatcgggccg ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca
     5221 ggataccggg gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt
     5281 ccgcggtatt ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa
     5341 agccaatcag gcactgaaag atgcggtagc ggaattaatg gaaaacgaag aaattcgcgg
     5401 gctgattatt ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac
     5461 actgcgtgtt tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga
     5521 cagccaggtg aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc agacttatca
     5581 ggtgctgcct actccgggcg cgaccccggc tgaaccgtta ccgccggggg agccaacgct
     5641 ttaacgctgg cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat
     5701 aaagcgactc tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcgcccac
     5761 gtcggtcaga gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa
     5821 ccacatcacc tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaggcgac
     5881 ttcaccacct tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt
     5941 gttcagcccc aggttacggc gtttacgctc actacctgcc cgcgctgcag cggcggaatc
     6001 gtggaatggc gggttacaca aggtcgcgtc gtattgctcg tttttatgga tgatgccgtt
     6061 aaaaattgcc ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt
     6121 agcactgata atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa
     6181 acgccagcca tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat
     6241 atccagaatg ctggcattcg ctggaattgt tccgcttgct tcagccagta aatcggcaag
     6301 gtgatgaata taatccgccc ggcccggtac tggtgggcag agaaaaccgt cggggatatc
     6361 ccagttcgct acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag
     6421 cggattggca aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag
     6481 ttcaggattg acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg
     6541 atgcaacccc ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc
     6601 cgcataacgc gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg
     6661 gctatggttt atggcaattc atcataaaaa tcagcattat gcatgtcatt aatttgtctt
     6721 attttgtaaa aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt
     6781 tgcatcgcgc aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca
     6841 ttatgaaaaa gtgcctcaca ctactgattg ccaccgtcct gagcggaatc tctctcacgg
     6901 cttatgccgc gcaaccgatg agtaacctgg acagcggtca gctacggccc gccggtaccg
     6961 tttcggcaac tggcgcatca aacctaagcg atctggagga taaactggca gaaaaagcgc
     7021 gcgaacaagg cgcgaaaggt tatgtcatta attccgcagg cggaaatgac cagatgttcg
     7081 gtactgcaac catctacaaa taaccccacc aacgtaactg cattcgcctc tggatgcagt
     7141 tttcgcctgt tttgtaatca aaaaacatca aatatgaact caatgtaaat aaatgtattt
     7201 ctttttcgcg caatgggtga tagaaaatcg cgccaagtga taatgcttat caaaattatt
     7261 atcacttgca cgaacactat cacgggatta acagtggcat cgcatccgca gagatgcttt
     7321 ctcgtggcag tgaaaatttc aacatataag aaaaagtcac ctgcaaaatg gaaaacaatc
     7381 gcaatttccc tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca
     7441 tcacgcctgt ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg
     7501 ttgtcgaagc atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct
     7561 cgcgtccggt agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag
     7621 atcagggcgc aaccaacctt accgacgcgc tcaaaaacgc ccccggcg
//
LOCUS       DAZOYS010000185         7625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOYS010000185 DAZOYS010000000
VERSION     DAZOYS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.186"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHUN9_003485"
     CDS             complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHUN9_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0696793.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(926..1492)
                     /locus_tag="ACHUN9_003486"
     CDS             complement(926..1492)
                     /locus_tag="ACHUN9_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0696794.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1708..3444
                     /gene="argS"
                     /locus_tag="ACHUN9_003487"
     CDS             1708..3444
                     /gene="argS"
                     /locus_tag="ACHUN9_003487"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0696795.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3486..3530)
                     /gene="yecV"
                     /locus_tag="ACHUN9_003488"
     CDS             complement(3486..3530)
                     /gene="yecV"
                     /locus_tag="ACHUN9_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0696796.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3621..4109
                     /locus_tag="ACHUN9_003489"
     CDS             3621..4109
                     /locus_tag="ACHUN9_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR0696797.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4229..4621)
                     /gene="flhe"
                     /locus_tag="ACHUN9_003490"
     CDS             complement(4229..4621)
                     /gene="flhe"
                     /locus_tag="ACHUN9_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR0696798.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4621..6699)
                     /gene="flhA"
                     /locus_tag="ACHUN9_003491"
     CDS             complement(4621..6699)
                     /gene="flhA"
                     /locus_tag="ACHUN9_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR0696799.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6692..>7625)
                     /gene="flhB"
                     /locus_tag="ACHUN9_003492"
     CDS             complement(6692..>7625)
                     /gene="flhB"
                     /locus_tag="ACHUN9_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0696800.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1938 a         2068 c         1867 g         1752 t
ORIGIN      
        1 aaaaatatta ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc
       61 gttcgttttt tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg
      121 gtacgagcaa gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg
      181 gcgttcaatg attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata
      241 ctcgtccgcg tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga
      301 ggcttgccac gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg
      361 caggttttct gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat
      421 aagttccata atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac
      481 ccgggcaatg cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc
      541 gaaggcgcga tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg
      601 tggcatatcg acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa
      661 ttcgcggacc gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa
      721 atcaccaccg cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga
      781 tttcagtacc cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac
      841 tctgtctgcg ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc
      901 cagtaatgcc atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa
      961 tggaccacgg atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg
     1021 gtaatcgctc atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga
     1081 gtccttcatc agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccagca
     1141 gaacgatttc aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta
     1201 gctccacaat agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac
     1261 aaatcggtct gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc
     1321 gacgccagcg ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca
     1381 gcggagtgat attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg
     1441 gtaaatccga tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc
     1501 ttcgtctttc agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt
     1561 tcacgctgct tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga
     1621 aaacagccat ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg
     1681 ctcgcttcat caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc
     1741 cgtcaggcca tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcattca
     1801 gcaaaagttc agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg
     1861 ggtatgacac cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc
     1921 gccagcaaag ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc
     1981 ctggctgaac atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa
     2041 aaacagacca ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt
     2101 cacctgcgct ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac
     2161 aaagtgattc gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca
     2221 tggctggaaa agcagcagca ggaaaacgcc ggtgatgaaa tggagctggc tgaccttgaa
     2281 ggtttctacc gcgatgcgaa aaagcattac gacgaagatg aagagtttgc cgagcgtgca
     2341 cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa
     2401 ctggtcgaca tcaccatgac gcagaaccag atcacctacg atcgtctcaa cgtgacgctg
     2461 acccgtgatg acgtgatggg cgaaagcctc tacaacccaa tgctgccagg aattgtggcg
     2521 gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt attccttgat
     2581 gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa agatggcggc
     2641 tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac actgcatgcc
     2701 gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca ggcatgggcg
     2761 atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc
     2821 atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg
     2881 gccgatctgc tggatgaagc cctggaacgt gcacgccgtc tggtggcaga aaagaacccg
     2941 gatatgccag ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa
     3001 tatgcggatc tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg
     3061 gcgtttgagg gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg
     3121 ttccgtaagg cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa
     3181 gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt
     3241 gcccgtgaag gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc
     3301 tctggcttct acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc
     3361 cgtctgaaac tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt
     3421 attgagacag tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta
     3481 cctgatcagg cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt
     3541 tctgactgtt ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt
     3601 tgaatttaag gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt
     3661 gccaggctta cgcagaagat acggttatag aaaacgacca tgacatttca gccatcaaag
     3721 attgttggca aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc
     3781 gacaagaata caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt
     3841 atatagaaca agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc
     3901 ttaaaaaatc acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt
     3961 caaatgagga cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta
     4021 tgatcataca gaccaatacg cgaactcgcc agttgcagct ataccgtaac tctgaagatt
     4081 tttatccaag ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag
     4141 ctattcctga taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat
     4201 actcgcgagc gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt
     4261 gtacctttag cggtggaatt aaccgcccac cgcctggcac ttcccagata aatcgcaacg
     4321 gttctgctgc cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga
     4381 cacaacgaga ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct
     4441 gataacgcca cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg
     4501 cagacgacat cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca
     4561 tcccctcccc ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca
     4621 ttatttgccg ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt
     4681 cgacaggacc actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag
     4741 cgcgtggttc accaacaata ctggcggcgc gcccagcatc tcctgacggg atagcgcttc
     4801 ctgagtttgc gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag
     4861 cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt
     4921 gccaggaaac cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa
     4981 ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat
     5041 atcgcgaatc ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag
     5101 cgtgacgacg ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc
     5161 caacagctgt tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg
     5221 gttaagatgc gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc
     5281 ctgttctttt agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt
     5341 cgcctcacca ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg
     5401 ataagcatca ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg
     5461 cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa
     5521 tttcttgcgg atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat
     5581 cgggatcagt cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca
     5641 cgtcgcttcg acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc
     5701 tttttgttcg cgtccgcgta tccaccaggc cagcccgagc aatccggcag tgaacagcaa
     5761 aaataccagg ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa
     5821 caacataacg cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc
     5881 ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ccggaatttg
     5941 tgccaccaga ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct
     6001 catgccatgt tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag
     6061 gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa
     6121 atcggcttcc tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc
     6181 agcgttaagg tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg
     6241 cgcacccact tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac
     6301 gagaatgaca aacaccacga taccgatagc gaaattgcca ccaacgagga agtgaccgaa
     6361 cgcttcgacc accttccctg ccgccgccgc gccggtatgc ccttccatca aaatgatacg
     6421 ggttgaagcc acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa
     6481 cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa
     6541 ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat
     6601 cgacaagatc aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag
     6661 gtttgcgggc aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa
     6721 aatccagggc ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca
     6781 gacgccagcg tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt
     6841 gacccgggat ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg
     6901 cttcaagcgt cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc
     6961 ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta
     7021 ccgaatagtg ggtcgggtta ttgacaatga catcagcttt cggcacatcg gccatcatcc
     7081 gacgccgtgc agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt
     7141 cgctttgttt gaactcatca cgaatatcct gccgcgacat acgcagcttt ttcaggtggc
     7201 tgaagatttg gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca
     7261 gtgcgcatag cccaaccaga tccatcgcat tacccatggc ggtagtcgga gactcggcca
     7321 ttaagcgcat catctgcggc cagtgatgcc agagataaaa ccccgtcacg ctgccaacca
     7381 ggatggtttt caaaattgcc ttaagcaact ccgcgccagt ctgtgccgag aacatccgtt
     7441 taatgcccgg tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata
     7501 ccagcccccc cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg
     7561 gcagcagcgc cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg
     7621 ggtca
//
LOCUS       DAZOYS010000186         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOYS010000186 DAZOYS010000000
VERSION     DAZOYS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.187"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHUN9_003493"
     CDS             171..701
                     /locus_tag="ACHUN9_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0696801.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHUN9_003494"
     CDS             837..1502
                     /locus_tag="ACHUN9_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696802.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHUN9_003495"
     CDS             1824..2447
                     /locus_tag="ACHUN9_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR0696803.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHUN9_003496"
     CDS             2532..2750
                     /locus_tag="ACHUN9_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0696804.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHUN9_003497"
     CDS             2728..3798
                     /locus_tag="ACHUN9_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0696805.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHUN9_003498"
     CDS             3933..5216
                     /locus_tag="ACHUN9_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0696806.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHUN9_003499"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHUN9_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0696807.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZOYS010000187         7574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOYS010000187 DAZOYS010000000
VERSION     DAZOYS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.188"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            63..1352
                     /gene="hpaG"
                     /locus_tag="ACHUN9_003500"
     CDS             63..1352
                     /gene="hpaG"
                     /locus_tag="ACHUN9_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0696808.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHUN9_003501"
     CDS             1349..2815
                     /gene="hpaE"
                     /locus_tag="ACHUN9_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0696809.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHUN9_003502"
     CDS             2817..3668
                     /gene="hpaD"
                     /locus_tag="ACHUN9_003502"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0696810.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3678..4058
                     /locus_tag="ACHUN9_003503"
     CDS             3678..4058
                     /locus_tag="ACHUN9_003503"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0696811.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHUN9_003504"
     CDS             4169..4972
                     /gene="hpaH"
                     /locus_tag="ACHUN9_003504"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0696812.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHUN9_003505"
     CDS             4983..5771
                     /gene="hpaI"
                     /locus_tag="ACHUN9_003505"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0696813.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHUN9_003506"
     CDS             5946..7322
                     /gene="hpaX"
                     /locus_tag="ACHUN9_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0696814.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7332..>7574
                     /locus_tag="ACHUN9_003507"
     CDS             7332..>7574
                     /locus_tag="ACHUN9_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0696815.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1738 a         2124 c         2150 g         1562 t
ORIGIN      
        1 tcgggaataa aagcaatcat taacatatta atgattaaga tgatcgaatc cgaggagtgg
       61 taatgaaagg cactatcttc gccgtagcgt tgaaccatcg cagccaactt gatgcatggc
      121 aggaagcgtt ccagcaatcc ccctacaaag ccccgcctaa aactgcggtc tggtttatta
      181 aaccgcgcaa tacggtgatt ggttgcggtg aaccgattcc ctttccacag ggtgaaaagg
      241 tactgagcgg tgcgactgtt gcgctgattg tgggaaaaac ggcgacgaaa gtacgtgaag
      301 aagatgcggc agagtacatc gccggatatg cgctggctaa cgacgtcagc ctgccggaag
      361 agagctttta ccgcccggca atcaaagcaa aatgtcgtga tggattctgc cccattggcg
      421 aaaccgtggc tctcagcaat gtcgataatc tgaccatcta taccgagatc aacgggcgtc
      481 ctgccgatca ctggaatacc gccgatttac aacgtaacgc cgcgcagttg ctgagcgccc
      541 tgagcgaatt tgccacgctg aatccaggcg acgccattct gctcggcacg ccacaggcgc
      601 gcgtggaaat acagccaggc gatcgcgttc gtgttctcgc agaaggtttc ccgccgctgg
      661 aaaatccggt agtggacgaa cgtgaagtga ccacgcacaa gagcttccca acgcagccac
      721 acccgcacgg cacgctgttt gccctcggcc tgaactacgc cgaccacgcc agcgaactgg
      781 aatttaagcc accggaagaa ccgctggtgt tcctgaaagc gccaaatacc ctcactggcg
      841 ataaccagac ctccgtgcgt ccaaacaata ttgaatacat gcactatgaa gcggagctgg
      901 tggtagttat tggcaaacag gcgcgtaacg tcagcgaagc cgatgccatg gattatgtcg
      961 cgggctacac cgtgtgtaac gactacgcca ttcgtgacta tctggaaaac tactaccgcc
     1021 ctaacctgcg ggtaaaaagc cgcgacggac tgacgccgat gctttcaacc atcgtgccga
     1081 aagaggcgat cccggacccg cataatctga cccttcgcac cttcgtcaac ggcgagttac
     1141 gccagcaagg caccaccgcc gatctgatct tcagcgtgcc cttcctgatc gcctacttaa
     1201 gtgaatttat gaccctgaat ccgggcgaca tgatcgccac cggcacaccc aaaggcttat
     1261 ctgacgtggt gcctggcgat gaagtagtgg tggaagtaga aggcgtgggc cgcctggtga
     1321 accgaattgt gagtgaggat acagcgaaat gaaaaaagta aatcattgga tcaacggcaa
     1381 aaatgttgca ggtaacgact acttccagac caccaatccg gcaacgggtg aagtgctggc
     1441 ggatgtggcc tctggcggtg aagcggagat caatcaggcg gtagcggcag cgaaagaggc
     1501 gttcccgaaa tgggccaatc tgccgatgaa agagcgtgcg cgcctgatgc gccgtctggg
     1561 cgatctgatc gaccagaacg tgccagagat cgccgcgatg gaaaccgcgg acaccggcct
     1621 gccgatccat cagaccaaaa atgtgttgat cccacgcgct tcccacaact ttgaattttt
     1681 cgcggaagtc tgccagcaga tgaacggcaa gacctatccg gttgacgaca agatgctcaa
     1741 ctacacgctg gtgcagccgg tgggcgtttg tgcgctggta tcgccgtgga acgtaccgtt
     1801 tatgaccgcc acatggaagg tcgcgccgtg tctggcgctg ggcaataccg cggtactgaa
     1861 aatgtcggaa ctctccccgc tgaccgctga cagcctgggt gagctggcgc tggaagccga
     1921 tattccggca ggcgtgctga acgtggtaca gggctacggc gcaaccgcag gggatgcgct
     1981 ggttcgtcat catgacgtac gtgccgtgtc gttcaccggc ggtacggcca ccgggcgcaa
     2041 catcatgaaa aacgccgggc tgaaaaaata ctccatggaa ctgggcggta aatcgccggt
     2101 gctgattttt gaagatgccg atattgaacg cgcgctggac gccgccctgt tcaccatctt
     2161 ctcgatcaac ggcgaacgct gcaccgccgg ttcgcgcatc tttattcagc aaagcatcta
     2221 cccggaattc gttaaacgct ttgccgaacg cgccaaccgt ctgcgcgtgg gcgatccgaa
     2281 cgatccgaat acccaggttg gcgcgcttat tagccagcag cactgggaaa aagtctccgg
     2341 ctatatccgt ctcggcattg aagaaggcgc aaccctgctg gcgggcggcc cggataaacc
     2401 gtccgacctg cctgcgcacc tgaaaggcgg caacttcctg cgcccaaccg tgctggcaga
     2461 cgttgataac cgtatgcgag tcgcccagga agagattttc gggccggtcg cctgcctgct
     2521 gccgtttaaa gacgaagccg aaggcttacg tctggcaaac gacgtggagt acggcctcgc
     2581 gtcgtacatc tggacacagg atgtcagcaa agtgttacgc ctggcgcgtg gcattgaagc
     2641 tggcatggtg ttcgtcaaca cccagaacgt gcgtgacctg cgccagccat ttggcggcgt
     2701 aaaagcctcc ggcaccgggc gtgaaggcgg tgagtacagc ttcgaagtgt tcgcggaaat
     2761 gaagaacgtc tgcatttcca tgggcgacca tccaattccg aaatggggag tctgatatgg
     2821 gtaagttagc gttagccgcc aagatcacgc acgtaccgtc gatgtatcac tctgaactgc
     2881 cagggaaaaa ccacggttgc cgccagggcg cgatcgacgg gcataaagag atcagcaagc
     2941 gttgccggga aatgggcgtc gataccatta tcgttttcga tacccactgg ctggtcaaca
     3001 gtgcttatca catcaactgt gcagaccatt ttgaaggcgt ctacaccagc aacgagctgc
     3061 cgcattttat ccgcgatatg acctacaact acgagggcaa cccggagttg gggcagctta
     3121 ttgccgatga agccttaaag ctcggcgtgc gggcaaaagc gcacaacatt cccagcctga
     3181 aactggaata cggcacgctg gtgccgatgc gctacatgaa tgaagacaag cacttcaaag
     3241 tggtctccat ttcggctttc tgcacggttc acgattttgc cgacagccgc aagctgggcg
     3301 aagcgattct gaaagcgatc gaacagtacg acggcaccgt ggcggtcctt gccagcggtt
     3361 cgttatcgca ccgctttatt gacgatcagc gtgcggaaga agggatgaac agctacaccc
     3421 gcgagttcga ccgccagatg gacgagcgcg tggtgaagtt gtggcgcgaa ggccagttca
     3481 aagagttctg caatatgctg ccggagtacg ccgactactg ctacggcgaa ggcaatatgc
     3541 acgacacggt gatgctgctg gggatgctcg gctgggataa atacgacggc aaggtggagt
     3601 ttatcaccga gctattccca agctctggca ccggtcaggt taacgctgtt ttcccgcttc
     3661 ccgcgtaagg acgttttatg ccgcacttta tcgttaaatg cagtgacaac atccgcgaag
     3721 aagccgacct gccggggttg ttcgccaaag tgaatccgac gctggcagcc acgggtattt
     3781 ttccgctggc gggtattcgc agccgcgtgc attgggtcga tacctggcag atggccgacg
     3841 ggcagcgtga ttatgccttc gtgcatatga cgttgaaaat cggcgcaggt cgcagcctgg
     3901 aaagccgcca gcaggcgggt gaaatgctgt ttgaactgat taaaacgcac ttcgccgccc
     3961 tgatggagag ccgcctgctg gcgttgtcgt ttgagattga agagctgcat ccgacgctga
     4021 attttaaaca aaacaacgtg cacgcattgt ttaagtagcg cgcagattgc ccggtggcgc
     4081 tgcgcttacc gggcctacaa aaccccaaac cgtacaccgt aggccggata aggcgcagcc
     4141 gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca ccctgatcgc
     4201 ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct cgctggatta
     4261 cccggagatc accatcgaag acgcttacgc ggtgcagcgt gaatgggttc gcctgaaaat
     4321 cgccgaaggt cgcacgctga aaggccacaa aatcggcctg acctcgaaag cgatgcaggc
     4381 aagctcgcag atcagcgaac cggattacgg cgcgctgctg gatgacatgt tcttccacga
     4441 tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg agctggcttt
     4501 cgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgtct acaacgccac
     4561 ggactacgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca tcgatccgga
     4621 aacccagcgc ccgcgcaaag tgttcgacac catttctgat aacgccgcca acgccggggt
     4681 gatcctcggc ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga tctccgccct
     4741 gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggcgtgc tgaatcatcc
     4801 ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac aactggaagc
     4861 cgggcaaatc attctcggtg gttcgttcac ccgcccggtt ccggcgcgta agggcgacac
     4921 cttccacgtc gattacggca acatgggttc cattagctgc cgctttgttt aaggagagaa
     4981 cgatggaaaa cagctttaaa gcggcgctga aagcaggccg tccgcagatc ggattatggc
     5041 tggggctgag tagcagctac agcgcagagt tactggccgg agcaggattc gactggttgt
     5101 tgatcgacgg tgagcacacg ccgaataacg tgcaaaccgt gctcacccag ctacaggcga
     5161 ttgcgcccta ccccagccag ccggtagtac gtccgtcgtg gaacgatccg gtgcaaatca
     5221 aacaactgct ggacgtcggc acacaaacct tgctggtgcc gatggtacaa aacgctgacg
     5281 aagcccgtga agcggtacgc gccacccgtt atccccccgc cggtattcgc ggtgtgggca
     5341 gtgcgctggc ccgcgcctcg cgctggaatc gtattcctga ttacctgcaa aaagccaacg
     5401 atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga
     5461 ttctggacgt ggaaggcgtc gacggcgtgt ttatcggccc ggcggatctg agcgccgata
     5521 tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag caggcgatcg
     5581 tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag caactggcaa
     5641 aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg
     5701 cccgcgccgc cgaagcgctg gcagcacggt ttggtgcgca ggccaccgcc gtgaagcccg
     5761 gcgtgtatta atgccggatg cgctgcgctt atccggccta cactcgcacc aaaccgtagg
     5821 ccggataagg cgtttacgcc gcatccggca aaaagccgta ccaaatcgcg gaccggataa
     5881 ggcgttacgc cgcatccgcc ataaaccttg tcgtacccta caaaaatccc attagaggaa
     5941 gaaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg aatcagcata
     6001 aagcgctgac tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgagcgtct
     6061 ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac
     6121 tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct accaccctgt
     6181 tctatgccgc ttatgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg
     6241 cgcggcgctg gatcgccacc atcatggtgc tctggggcat cgcctctact gccaccatgt
     6301 ttgccactgg ccccaccagc ttgtacgtac tgcgtatact ggttggcatt accgaagccg
     6361 gctttctgcc tggtattctg ctgtatttaa ccttctggtt tccggcctat ttccgcgccc
     6421 gtgccaacgc cttgtttatg gtagcaatgc cggtaacgac agcgttgggc tcgatcgttt
     6481 ccggctacat tttgtcgctg gatggcgtca tggcattaaa aggctggcag tggctgtttt
     6541 tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact
     6601 caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg
     6661 ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac
     6721 aacgcagtat gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt
     6781 tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagtt
     6841 ttaatcaggg cagcagtaat atcaccatcg gcatgctggc cgccgtaccg cagatttgta
     6901 ccattctcgg gatggtctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc
     6961 acaccgccct tccttatttg ttcgctgccg caggttggtt actggcttcg gcaactgatc
     7021 acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg
     7081 cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg
     7141 cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt
     7201 tgaaagattt gaccggcagc tttaacagtg gattgtggtt tgttgccgcg ctgctggtga
     7261 ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccctgt
     7321 aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg
     7381 aaagcctggg cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gcattttttg
     7441 gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg
     7501 gacagattga gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc
     7561 tgacgccgcc gtca
//
LOCUS       DAZOYS010000188         7555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOYS010000188 DAZOYS010000000
VERSION     DAZOYS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.189"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHUN9_003508"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHUN9_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0696816.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHUN9_003509"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHUN9_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0696817.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHUN9_003510"
     CDS             1086..1676
                     /locus_tag="ACHUN9_003510"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0696818.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUN9_003511"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUN9_003511"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0696819.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUN9_003512"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUN9_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0696820.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHUN9_003513"
     CDS             complement(3788..3961)
                     /locus_tag="ACHUN9_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0696821.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHUN9_003514"
     CDS             4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHUN9_003514"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0696822.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4951..5178
                     /locus_tag="ACHUN9_003515"
     CDS             4951..5178
                     /locus_tag="ACHUN9_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0696823.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHUN9_003516"
     CDS             complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHUN9_003516"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0696824.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6751..7555)
                     /locus_tag="ACHUN9_003517"
                     /pseudo
     CDS             complement(6751..7555)
                     /locus_tag="ACHUN9_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1756 a         1903 c         1950 g         1946 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac cttgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatcaggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt ggtataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
     4381 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt
     4441 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
     4501 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
     4561 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
     4621 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
     4681 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
     4741 ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
     4801 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
     4861 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
     4921 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
     4981 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
     5041 gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag cgatgaagtg
     5101 ttagtcaaag cctttgctaa ggccgataaa gattcgcttt tttggggcga acagaccatc
     5161 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt ttctgttatt
     5221 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
     5281 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
     5341 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
     5401 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
     5461 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
     5521 agtcgtgccc aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt
     5581 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
     5641 ggctgtcctg gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga
     5701 tttctcccca ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat
     5761 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg
     5821 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
     5881 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
     5941 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
     6001 tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat
     6061 cacaccccgg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
     6121 tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct
     6181 gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg
     6241 tggtgagttg cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca
     6301 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
     6361 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta
     6421 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
     6481 atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct
     6541 tgttgaacgc ctgtttttct atatatatct tcaaaaacag gcaaggtttg acgcaaacta
     6601 tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa
     6661 tatcagcggc gttactaata cagaggcaat agtaccaata ataagagaag tgaggctaat
     6721 aacaatggtt tttagcacat gcctttgtca ttaaattgca taacgagtta aaaaatcaat
     6781 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
     6841 gtaaacgtca taatgattgc cagtgtttat taaataccca tactcaaggt cgtcatctgt
     6901 tgaaaaggat tcattcccta aggagattga tgccacattg ctgtaaacaa taaataatga
     6961 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcattaacct tctgctctgg
     7021 gtcggtgtct aaaacaaggc gtgccttgtt atcattaaat ataaattcgg gtaaatgcag
     7081 tatgcatttt ttgtcattca agcaactatc aataaattct tttttaaaag acaaaaaact
     7141 ccatgataaa aaaccaccct gcccctgaat tttgttgtca gattgtagat taacgccaaa
     7201 taacgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag cctcgatcgt
     7261 ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct tatggccgcc
     7321 cagcgcccat gaatactcat gttcttttaa aaccagtatc taaatcagat attcccggcg
     7381 tatttatctg ttctggctgg atactcatat tttcatcatc taaacaatac tcagaaaaaa
     7441 tatcatcaca cgcagtaatg ttttcaatat attccataaa gccataccaa taataacctt
     7501 taaaatctgt gatagactct gaacctctgc ccggcaagga cactctaaca atatc
//
LOCUS       DAZOYS010000189         7469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOYS010000189 DAZOYS010000000
VERSION     DAZOYS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.190"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUN9_003518"
     CDS             <1..108
                     /locus_tag="ACHUN9_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0696825.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUN9_003519"
     CDS             complement(149..1231)
                     /locus_tag="ACHUN9_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0696826.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUN9_003520"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUN9_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0696827.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUN9_003521"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUN9_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696828.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUN9_003522"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUN9_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0696829.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUN9_003523"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUN9_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0696830.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUN9_003524"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUN9_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0696831.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUN9_003525"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUN9_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0696832.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUN9_003526"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUN9_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0696833.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..>7469)
                     /locus_tag="ACHUN9_003527"
     CDS             complement(7185..>7469)
                     /locus_tag="ACHUN9_003527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696834.1"
                     /translation="THHIGRIMNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAK
                     LTGEKILRWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
BASE COUNT         1821 a         2043 c         1551 g         2054 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgt
//
LOCUS       DAZOYS010000190         7336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOYS010000190 DAZOYS010000000
VERSION     DAZOYS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.191"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            205..447
                     /gene="yiiF"
                     /locus_tag="ACHUN9_003528"
     CDS             205..447
                     /gene="yiiF"
                     /locus_tag="ACHUN9_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0696835.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(777..1706)
                     /gene="fdhE"
                     /locus_tag="ACHUN9_003529"
     CDS             complement(777..1706)
                     /gene="fdhE"
                     /locus_tag="ACHUN9_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0696836.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1703..2338)
                     /gene="fdoI"
                     /locus_tag="ACHUN9_003530"
     CDS             complement(1703..2338)
                     /gene="fdoI"
                     /locus_tag="ACHUN9_003530"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0696837.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2335..3237)
                     /gene="fdoH"
                     /locus_tag="ACHUN9_003531"
     CDS             complement(2335..3237)
                     /gene="fdoH"
                     /locus_tag="ACHUN9_003531"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0696838.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3250..6300)
                     /gene="fdnG"
                     /locus_tag="ACHUN9_003532"
     CDS             complement(3250..6300)
                     /gene="fdnG"
                     /locus_tag="ACHUN9_003532"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5713..5715),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0696839.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6494..7327
                     /gene="fdhD"
                     /locus_tag="ACHUN9_003533"
     CDS             6494..7327
                     /gene="fdhD"
                     /locus_tag="ACHUN9_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0696840.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1655 a         1947 c         1967 g         1767 t
ORIGIN      
        1 ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat ttttgagtaa
       61 taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa aaataaaaaa
      121 tgatgcgtaa atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc
      181 aaaaatgata ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt
      241 ttactcgatt tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat
      301 cttcctcgtg cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa
      361 acagcaagaa ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat
      421 cagcgtaagc tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca
      481 acgtgcgcgt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg
      541 caaattcaat atattgaagt ttattggtag gcctgataag cgcagcgcat caggcaatca
      601 tacgcttgtc atcagtgaca tacccataac cttatcgatg tgttggaggg aagaacgagg
      661 caaacagaca attgcggcat gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg
      721 tcacacccta tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac
      781 tctccttcac ccggaaacag gaacgggtta atggaactac gggcgtagcc ttcttgctcc
      841 attcgcgcgt ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct
      901 ttttcctgat aaagaatctt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc
      961 gcggcctgtt cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg
     1021 cattttacgc gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga
     1081 ccctgagtgg tgccaatttg caccatgctg gacaccggca tagagccaca taccgggcaa
     1141 tattgacgtt gttcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc
     1201 gcccagtaga gcgacagcgc agcccagata aacggcgctt tatcgctgct gacggacgag
     1261 aaatcagagg caaacagcgc gctggccata tcttccagct cctgagtcga tgccttctcc
     1321 agattctcaa tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaattagc
     1381 gccatcagca gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc
     1441 ttgccctgtg cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg
     1501 tacagcacca cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc
     1561 ggattatttt ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc
     1621 cgagggaaca ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca
     1681 tcttgcggga ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac
     1741 ggtaccagcg cgggtgatgt ttcttcgccc aggcgctggt aacccatcct tccaccatgg
     1801 cggtaatcgt gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta
     1861 acgccactgc ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag
     1921 caggcgcaaa ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa
     1981 taatcgccgc ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt
     2041 cctcgttgac gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta
     2101 ggttgtgatg ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga
     2161 caaacgggtg cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga
     2221 aggacgggaa caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga
     2281 tccagtggtt gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt
     2341 gcgctcctcg tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc
     2401 gacacccacg tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag
     2461 cggtttccag atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa
     2521 gccatgatac agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc
     2581 gacgcctgcc ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac
     2641 gcgctcgctc gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg
     2701 gcaggtcttc acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca
     2761 tttgtagacg cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat
     2821 gcaataaccg cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg
     2881 aataattgca ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca
     2941 gccgtctttg cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat
     3001 caccgtccac gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc
     3061 gacggtatcg cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat
     3121 acaggtggtg acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc
     3181 gggggtcaga ccgttagtcg cggaacgacg aatgatatct tgcgattgat aagccatatg
     3241 tcgtctccgt tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt
     3301 gttcgcatca ccaacgaatg gcgttaacgt attggcaata aagccttttt tagctacgcc
     3361 ttcatagccc cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag
     3421 cgtgcgaata cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac
     3481 tttcacggta tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac
     3541 aaactgctct ggttgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg
     3601 ctcggtcaga cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc
     3661 ggcgtcgtct ttaaagattc gcgcagccgg gttcgagata acgtttggat gcagcgggtt
     3721 agttcccagc ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt
     3781 atcgagggca aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct
     3841 gcccggaggc gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca
     3901 tttcagcaac tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt
     3961 atacagaatg cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc
     4021 agacggatcg gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc
     4081 gaaaatccag cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag
     4141 ttgttggcct ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc
     4201 cttaccgttg ctctccatcg ccacttcttc cgacttcggc tcatgcggaa tggcgtagtt
     4261 ccaggtcatg ttcagcacct gatccgggtt tgcgccaccc tgttcggcat acatcttgcg
     4321 caagcgcagg aagataccgg agaggatctc gccatcagtc agcgcaatcc ctggggcgtc
     4381 cgcacctttc cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc
     4441 tgcgaagcag gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc
     4501 gttcagctca ccgtggttct gccagaagtt agaggtttca gtgttcagcg ggtcgatggt
     4561 gacgaggaac ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc
     4621 aacagggtta aagccctggc agatatagcc attgaccttg ccctctttca tcatctcgaa
     4681 gtattgcagg acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt
     4741 ttccgccgtc gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta
     4801 gttgccccag tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta
     4861 ggtttgcaga tcggtctgct tctcgcttgg cagcgtcatg taacctggca ggctctgcga
     4921 cagcagcccc aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc
     4981 gccgcctgcc atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt
     5041 ttgcgcacca acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc
     5101 gtgagcactg gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc
     5161 acagatgttt tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca
     5221 cacgcagcgc ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc
     5281 gtaagtccag gaggatttat cgtacttgcg tttttccgcg tcatagccgg tgaacaggcc
     5341 atcttcaaag ccgtaatcct cacgcacgat caggctggcg ttggtatagg cttcggtgta
     5401 ttcgcggttg aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat
     5461 gtcagtaccg gaacgaatag gggcatagta gtcagccacc gccgccgtac gcgtaaagcg
     5521 aggatcgatc acaatcagct tcgcgccatt gtgaattttg gcttccatcg cccagcggaa
     5581 cccgaccggg tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt
     5641 gatgtcgacc cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac
     5701 cgttggtccg tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga
     5761 gaatttttgc gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt
     5821 ggagagccag cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg
     5881 gtcttctttc atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca
     5941 tttatcagaa cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa
     6001 atccaccagg ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc
     6061 gccttcgata tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat
     6121 caacagccca cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag
     6181 cagtttatac tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc
     6241 cgtggtgcct gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat
     6301 ggattgctcc ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag
     6361 ggagtgggag ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt
     6421 ccctccatcc aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc
     6481 tggttaagag aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga caaatatcac
     6541 aggtgttcgt caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga
     6601 ggtcgcggaa gaagttcccg ttgcgctggt ctacaacggc atttcgcatg tggtgatgat
     6661 ggcgtcgccc aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat
     6721 agaaagtccg cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt
     6781 acaaattgag ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc
     6841 gggacgtacg ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt
     6901 gcagccgcta ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca
     6961 tctcaacgat tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat
     7021 gttgccatct ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga
     7081 caaactgtta ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt
     7141 ttccagtcgt gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt
     7201 gtttgcggtg tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac
     7261 actggtaggt ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag
     7321 caattaatta aaataa
//
LOCUS       DAZOYS010000191         7324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOYS010000191 DAZOYS010000000
VERSION     DAZOYS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.192"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHUN9_003534"
     CDS             complement(<1..959)
                     /locus_tag="ACHUN9_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0696841.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..1808)
                     /locus_tag="ACHUN9_003535"
     CDS             complement(1560..1808)
                     /locus_tag="ACHUN9_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0696842.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2071..2166)
                     /locus_tag="ACHUN9_003536"
                     /pseudo
     CDS             complement(2071..2166)
                     /locus_tag="ACHUN9_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2163..2729)
                     /locus_tag="ACHUN9_003537"
     CDS             complement(2163..2729)
                     /locus_tag="ACHUN9_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0696843.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUN9_003538"
     CDS             3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUN9_003538"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0696844.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUN9_003539"
     CDS             4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUN9_003539"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0696845.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5410..6150
                     /locus_tag="ACHUN9_003540"
     CDS             5410..6150
                     /locus_tag="ACHUN9_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0696846.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUN9_003541"
     CDS             6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUN9_003541"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0696847.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6708..7310)
                     /locus_tag="ACHUN9_003542"
     CDS             complement(6708..7310)
                     /locus_tag="ACHUN9_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0696848.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1854 a         1643 c         1926 g         1901 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt
     1741 aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat gcagatttca
     1801 atacgcataa aaatccctct acactgtgtt tttatacagt attattttta actgtatgga
     1861 taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg caaaaatatt
     1921 tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt
     1981 ttcatatggc atggcaatcc aacgctttga ttattctaaa gaaaacacat gctcttgggc
     2041 gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg catactcttc
     2101 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     2161 aatcataacg cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     2221 tgattaatgc tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc
     2281 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc
     2341 gagatcccac ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc
     2401 gtaccgtaaa acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct
     2461 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga gggggcatca
     2521 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
     2581 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     2641 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
     2701 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
     2761 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
     2821 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     2881 acgaggcgtt acagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     2941 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     3001 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     3061 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     3121 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     3181 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     3241 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     3301 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     3361 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     3421 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     3481 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     3541 ccacgccgga aggcgcgcgt gactacttgg tgccttctcg tgtgcacaaa ggtaaattct
     3601 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     3661 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     3721 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     3781 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     3841 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     3901 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     3961 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     4021 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     4081 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     4141 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     4201 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     4261 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     4321 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     4381 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     4441 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     4501 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     4561 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     4621 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     4681 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     4741 cgagctttgc taacccgact gtactggctg agctgagcat tcaggttgtg aagaaggctg
     4801 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     4861 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     4921 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     4981 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     5041 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     5101 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     5161 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     5221 atacggaatc atggttctgt cttgcgctcc cgcacgaacg ccaggtagtc ttcactgaac
     5281 atctggctta caagtggctt gatgcgcctg ctgccgcggc gctcactaag tcctggagca
     5341 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     5401 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     5461 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     5521 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     5581 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     5641 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     5701 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattt
     5761 agcaaatgtg gcggtaacct cggtactgat ggttccgttg cctatctgtt cagcaaaaaa
     5821 ggcgtgatct ccttcgagaa aggcgatgaa gacaccatta tggaagcagc actggaagca
     5881 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg acgtctacac tgcatgggaa
     5941 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     6001 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcacc gaaactgatg
     6061 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtctacca taacggtgaa
     6121 atctctgatg aggttgcagc aactctctga tggggcctgc taaacagcaa aacggagacg
     6181 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgatcggct atggtgtcat
     6241 ccgccaggta ggcaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
     6301 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     6361 ccagccagat tatttcgcca ttgaacaagt ctttatggcc aaaaatgccg actcggctct
     6421 gaaactgggg caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg aattgccggt
     6481 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
     6541 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     6601 tgccgccgat gcgttagcta tcgcgattac ccattgtcac gtcagccaga atgcaatgca
     6661 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     6721 gtcagttcag gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccag acccggatga
     6781 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     6841 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttctcc aaacgtttct
     6901 atttcaccca ccggaatgca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
     6961 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
     7021 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
     7081 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
     7141 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
     7201 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
     7261 gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac gttgtcattc
     7321 catt
//
LOCUS       DAZOYS010000192         7265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOYS010000192 DAZOYS010000000
VERSION     DAZOYS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.193"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHUN9_003543"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHUN9_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHUN9_003544"
     CDS             complement(4539..5000)
                     /locus_tag="ACHUN9_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0696849.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6930)
                     /locus_tag="ACHUN9_003545"
                     /pseudo
     CDS             complement(5028..6930)
                     /locus_tag="ACHUN9_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1378 a         2206 c         1961 g         1720 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccctggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcggggc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca ggttgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg ggggggcggt tttcctggtg
     5581 gtaggtgcgc cccataatga tcggctggtc cggatcgccg ttgaggaagt ctacaatcac
     5641 ttcctgaccc acacgcggta tcgccaggtg gccaaaaccg gtgcctgccc acgcctgtgc
     5701 cacacggatc cagcacgaac tgtcttggtc tgccgggtta taacggtccc agttgaactt
     5761 cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg cctgccggtc ctgtcacgac
     5821 agcgctctgc gggccgtcga ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc
     5881 tgccgggatc accgcaaaat ggttctccag cgccgtcccc gctccctgcc gtcctggcac
     5941 cgcctgtggc tgttcgccgt gcagttcact tgccaccacc tgccattccc ggttcaggtt
     6001 cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc catatctccg gcgagcgact
     6061 cattccccgc gcggtttctg cattgtttcg ccagccgtcc atctgccagc gggcaaagtt
     6121 ctgcccgtga gcacccttga aacgtccggg gtagtcatac acttcatact gcgtgcgctg
     6181 gtagtcctgg tgctggcctt cctgttcaaa acgtccggcc cagccggggc gtttaaaggt
     6241 gtagtctttg gtcaccacgg aagaagggcg gatttgtgcg ctgtagcgga actggctgat
     6301 gcagagggtg ctcacctcgg tacgggtgtt cgggttccat gggatttcaa aagattcggg
     6361 cagatggcgg actgtgtcgc acagcaccag gctctggtcg gtacttttgt aagcatgctc
     6421 ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt aatcagtctc
     6481 accgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg gactccattc
     6541 ggtcaccccg ttttcctgca acatcgtgcc gaggatgctt ttgatgtcct cgttctggaa
     6601 gatacggaag ttctggcgca gaccggcacg ccacagcggc gggtgcacct tcatgctgta
     6661 cagcatctgg tttttgtcgt tctccccgag ttcaaaccag gtcaccacgc ctttcacccg
     6721 gcgctgtact tcatcgccct gccatatcgt caggtaggcc attttgtcca gcacctgcgc
     6781 aaattcaagg gagagaaact gctggctgac cagggagaga tcgagggaaa agagcgaaga
     6841 gagtgactgg ttcagatgaa aggaaaccac cgcaaaagcg tccggcggca ggccgtccac
     6901 ttccagtgtg aaacgtaatc cggttgacat agcaacctcc agtaaaatga ataatgatcc
     6961 gtaccgtaca actgcggata atgtgataag cgggacgact tcagacgtta ttagggaagg
     7021 aacagaaaat gctatggtta tgtgagaatt ttgtttaaat attactttac agaaacgggt
     7081 gtctgttcag tcaaaaaaat aatcacaagc tcaacctctt gtttcttggc gttattcagg
     7141 acaggtacgc atatcacaac gagtaacaaa aacgcaaagt taaaccaata ttcataatat
     7201 atttttattc acagcaataa ataatccaat taacattatt aaaatgaatg attgcataag
     7261 aaata
//
LOCUS       DAZOYS010000193         7254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOYS010000193 DAZOYS010000000
VERSION     DAZOYS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.194"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(235..1098)
                     /locus_tag="ACHUN9_003546"
     CDS             complement(235..1098)
                     /locus_tag="ACHUN9_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0696850.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(1070..2620)
                     /locus_tag="ACHUN9_003547"
     CDS             complement(1070..2620)
                     /locus_tag="ACHUN9_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0696851.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2878..3657
                     /gene="deoC"
                     /locus_tag="ACHUN9_003548"
     CDS             2878..3657
                     /gene="deoC"
                     /locus_tag="ACHUN9_003548"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0696852.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3784..5106
                     /gene="deoA"
                     /locus_tag="ACHUN9_003549"
     CDS             3784..5106
                     /gene="deoA"
                     /locus_tag="ACHUN9_003549"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0696853.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5158..6381
                     /gene="deoB"
                     /locus_tag="ACHUN9_003550"
     CDS             5158..6381
                     /gene="deoB"
                     /locus_tag="ACHUN9_003550"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0696854.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6461..7180
                     /gene="deoD"
                     /locus_tag="ACHUN9_003551"
     CDS             6461..7180
                     /gene="deoD"
                     /locus_tag="ACHUN9_003551"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0696855.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1696 a         1918 c         1974 g         1666 t
ORIGIN      
        1 gtctgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca
       61 atgcctgatg cgacgctgac gcgtcttatc atgcctacaa gtctgtgccg aaccgtaggc
      121 cggataaggc gttcacgccg catccggcag tcggcgcata atgcctgatg cgacgcttgt
      181 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccgtatccgg catgtcacaa
      241 atagagcgcc ggaaatatca accggctcac cccgcgcacc tttaacgcat cagccaacgg
      301 ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc tcgccataca cgccgtgggc
      361 atgaaacgca ttcaggcgaa ccggaacatc gccaagtctc ttgataaacg tcgccagctc
      421 atcgatgtga tgcaaataat ccacctggtc tggaattacc agcaaacgca gttccgccag
      481 cttgccgcgc tctgcaagca aacagatgct gcgcttaatc tgctgattat cgcgtccggt
      541 taagtgttga tgacattcgc tcccccacgc tttgagatcg agcattgcgc cgtcacacac
      601 cgggagtaat ttttcccagc cggtttcgct caacatgccg ttactgtcca ccaggcaggt
      661 gaggtggcgc agttgcggat cgtttttgat agcagtaaac agcgccacta caaacggcag
      721 ttgggtcgtg gcttcgccgc cgcttaccgt tatcccttca ataaacagca ccgctttgcg
      781 gacatggcta agcacttcgt ccacgctcat ggattgcgcc attggcgtgg catgttgcgg
      841 acacctcttc aggcaggtat cacactgctc gcaaaccaca gcgttccaca ccactttgcc
      901 gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg cactccccac agtcattgca
      961 acgtcccatc gtccacggat tgtgacagtt tttgcagcgc agattgcagc cctgcaaaaa
     1021 cagagccaga cgactgcctg gcccgtcaac gcaggagaag gggataatct tactgactaa
     1081 agcgcatctg ctgttcatgg cttatcacgc gcggctggcg ttccagaata cgagtgttgc
     1141 gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga accttcggcg cgatattttt
     1201 ctaaatccga caaacgcacc atataaccgg taacgcgaac cagatcgtta ccgctgacat
     1261 tggcagtaaa ttcacgcatt ccggctttaa aggcaccgag gcaaagctgt accagcgcct
     1321 gcggattacg tttgatggtt tcgtcgagcg tcagaatgtc gctgatgccg gaataataat
     1381 aagcatgatg cggggcgaca gtttgcagat gggtgatcgg atctggctca tcaccatacg
     1441 gcaaacgcgc gcccggcgtg gtgccgatat cggaactgat ccccgactgt gcgtgtaaca
     1501 tggcgcgttt ttgccagcca tatttcacgg gggtattggc gacaaactcc gccagttgcg
     1561 cgctgatgcg ataacccact tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc
     1621 cttctttctt acacagcaag ttaaccgctt ccgccagccc atacatgcca aacattggca
     1681 caaaacgttc agggttaatc agcccttctt tcaccaggaa gctattctca aagaagtgtg
     1741 attgttgata gaggaattca caccgcgcat cgatgatggc gatctgctgc tggcagtagt
     1801 gcggtagagt gcgcgtaaag aagtcatcca gcgattcgct gcgctcggca atggctttca
     1861 ggttaaggcg taccagcgtg ctgccaccac ccgccagcgg cagtgagttg taacagctca
     1921 caatcccgta gccccctttt gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg
     1981 gtttgctaca ttcacagatg ttcttcgcca cttccagcag caggtcatca ggggtgattt
     2041 caggatcgta gataaaggtc aggttcggtg aaacctgctt caactctgcg tctgcacgta
     2101 agatcgcgcg ggtaatgggc gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg
     2161 gcagggttct gtcgaggtaa cgccagaaac gttttattcg aacatcgatc tcgtcttgtg
     2221 ttagaattct aacatatggt tgcaacaacg catccagttg ccccaggtag accggcatcg
     2281 atgtgaccga cggtacgtgg tggtaaagaa tggtcagcag agagagtgcg tcatcaagat
     2341 ctttcgcgcc ttccagctcc agccattcgg aaccgttcgc cagaaaacgg gcgtaatcgg
     2401 gtaagacata gcgcggtttg tacggcgcat gaccttcaaa catatcgcag attacaccct
     2461 catccagcgc gcggcgggct tcggcaggaa gctgcggata aggcagattg ttttctgctt
     2521 ccagtgccag aaaatggcgc ttctgctccg ggctaagcac tgggctggtg acaatttgct
     2581 ggcaacgttg ttgcagtgca ttttcatgag aagtgggcat cttcttttcc ttttatgccg
     2641 aaggtgatgc gccattgtaa gaagtttcgt gatgttcact ttgatcctga tgcgtttgcc
     2701 accactgacg cattcatttg aaagtgaatt atttgaacca gatcgcatta cagtgatgca
     2761 aacttgtaag tagatttcct taattgtgat gtatatcgaa gtgtgttgcg gagtagatgt
     2821 tagaatacta acaaactcgc aaggtgaatt ttattggcga caagccagga gaatgaaatg
     2881 actgatctga aagcaagcag cctgcgtgca ctgaaattga tggacctgac caccctgaat
     2941 gacgacgaca ccgacgagaa agtaattgct ctgtgtcatc aggccaaaac cccggtcggc
     3001 aataccgccg ctatctgtat ctatcctcgc tttatcccga ttgctcgcaa aacgctgaaa
     3061 gagcagggca ccccggaaat ccgaatcgct acggtaacca acttcccaca cggtaacgat
     3121 gacatcgaaa tcgcgctggc agaaacccgt gcggcaatcg cctacggtgc tgatgaagtt
     3181 gacgttgtgt tcccgtaccg cgcgctgatg gcgggtaacg agcaggttgg ttttgacctg
     3241 gtgaaagcct gtaaagaggc ttgcgcggca gcgaatgtac tgctgaaagt gatcatcgaa
     3301 accggcgaac tgaaagacga agcgctgatc cgtaaagcgt ctgaaatctc catcaaagcg
     3361 ggtgcggact tcatcaaaac ctctaccggt aaagtggctg tgaacgcgac gccggaaagc
     3421 gcgcgcatca tgatggaagt gatccgtgat atgggcgtag aaaaaaccgt tggtttcaaa
     3481 ccagcgggcg gcgtgcgtac tgcggaagat gcgcagaaat atctcgccat cgcagatgaa
     3541 ctgttcggtg ctgactgggc agatgcgcgt cactaccgct ttggtgcttc cagcctgctg
     3601 gcaagcctgt tgaaagcgct gggccacggt gacggtaaga gcgccagcag ctactaagta
     3661 agatgcttta cgcctgatgc gctgcgctta tcaggcctac gagacgtatc tacccgtagg
     3721 ccggataagg cgtagacgca tccggcaaaa gccgcctcat actcttttcc tcgggaggtt
     3781 accttgtttc tcgcacaaga aattattcgt aaaaaacgtg atggtcatgc actgagcgat
     3841 gaagaaattc gtttctttat caacggcatt cgcgacaaca ctatctccga agggcagatt
     3901 gccgccctcg cgatgaccat tttcttccac gatatgacaa tgcctgagcg tgtctcgctg
     3961 accatggcga tgcgagattc aggaaccgtt ctcgactgga aaagcctgca tctgaatggc
     4021 ccgattgttg ataaacattc cactggtggc gtcggcgatg tgacttcgct gatgttgggg
     4081 ccgatggtcg cagcctgcgg cggctatatt ccgatgatct ctggtcgcgg cctcggtcat
     4141 actggcggta cgctcgacaa actggaatcc atccctggct tcgacatttt cccggatgac
     4201 aaccgtttcc gcgaaattat taaagacgtc ggcgtggcga ttatcggtca gaccagctca
     4261 ctggctccgg cggataaacg tttctacgcg acccgtgata ttaccgcaac cgtggactcc
     4321 atcccgctga tcaccgcctc tattctggcg aagaaacttg cggaaggtct ggacgcgctg
     4381 gtgatggacg tgaaagtggg tagcggcgcg tttatgccga cctacgaact ctctgaagcc
     4441 cttgccgaag cgattgttgg cgtggctaac ggcgctggcg tgcgcaccac cgcgctgctc
     4501 accgacatga atcaggtact ggcctccagt gcaggtaacg cggttgaagt tcgtgaagcg
     4561 gtgcagttcc tgacgggtga gtatcgtaac ccgcgtctgt ttgatgtcac gatggcgctg
     4621 tgcgtggaga tgcttatctc cggcaaactg gcgaaagatg acgccgaagc gcgcgcgaaa
     4681 ttgcaagcag tgctggacaa cggtaaagca gcagaagtct ttggtcgtat ggtagcggca
     4741 caaaaaggcc caaccgactt cgttgagaac tacgcgaagt atctgccgac agcgatgctg
     4801 acgaaagcag tctatgctga taccgaaggt tttgtcagtg aaatggatac ccgcgcgctg
     4861 gggatggcag tggttgcaat gggcggcggt cgtcgtcagg catctgacac cattgattac
     4921 agcgtcggct ttactgatat ggcgcgtttg ggcgaccagg tagacggtca gcgtccgctg
     4981 gcggttatcc acgcgaaaga cgaaaacagc tggcaggaag cggcgaaagc ggtgaaagcg
     5041 gcaattaaac ttgccgataa agcaccggaa agcacaccaa ctgtctatcg ccgtatcagc
     5101 gaataacggt atactgatct gatcatttaa atttgaagca ctgagtacgg agaacatatg
     5161 aaacgtgcat ttattatggt gctggactca ttcggcatcg gcgctacaga agatgcagaa
     5221 cgctttggtg acgtcggggc tgacaccctg ggtcatatcg cagaagcttg tgccaaaggc
     5281 gaagctgata acggtcgtaa aggtccgctc aatctgccaa atctgacccg tctggggctg
     5341 gcgaaagcac acgaaggttc taccggtttc attccggcgg gaatggacgg caacgctgaa
     5401 gttatcggcg cgtacgcatg ggcgcacgaa atgtcatccg gtaaagatac cccgtctggt
     5461 cactgggaaa tcgccggtgt cccggttcta tttgagtggg gatatttctc cgatcacgaa
     5521 aacagcttcc cgcaagagct gctggataaa ctggttgaac gcgctaatct gccgggttac
     5581 ctcggtaact gccactcttc cggtacggtc attctggatc agctgggcga agagcacatg
     5641 aaaaccggca agccgatttt ctatacctcc gctgactctg tgttccagat tgcctgccac
     5701 gaagaaacct ttggcctgga taaactctac gaactgtgcg aaatcgcccg tgaagagctg
     5761 accaacggcg gctacaacat cggtcgtgtt atcgctcgtc cgtttatcgg tgacaaagcc
     5821 ggtaacttcc agcgtaccgg taaccgtcac gacctggctg ttgagccgcc agcaccgacc
     5881 gtgctgcaga aactggttga tgaaaaacac ggtcaggtgg tttctgtcgg taaaattgca
     5941 gacatctacg ccaactgcgg tatcaccaag aaagtgaaag caactggcct ggacgcgctg
     6001 tttgacgcca ccatcaaaga gatgaaagaa gcgggtgata acaccatcgt cttcaccaac
     6061 ttcgttgact tcgactcttc ctggggccac cgtcgcgacg tcgccggtta tgctgtgggt
     6121 ctggagctgt tcgaccgccg tctgccggag ctgatgtctc tgctgcgcga tgacgacatc
     6181 ctgatcctca ctgctgacca cggttgtgat ccgacctgga ccggtactga ccacacgcgt
     6241 gaacacattc cggtactggt ttacggcccg aaagtaaaac cgggctcact gggtcaccgt
     6301 gaaaccttcg cggatatcgg ccagactctg gcaaaatatt ttggtacttc tgatatggaa
     6361 tatggcaaag ccatgttctg ataatgggtg cggcttgatg ccctcacccc ggccctctcc
     6421 ctcagggaga gggtgaaaac atgttgataa ggataaaaca atggctaccc cacacattaa
     6481 tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca ggcgacccgc tgcgtgcgaa
     6541 gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg aacaacgttc gcggtatgct
     6601 gggcttcacc ggtacttaca aaggccgcaa aatttccgta atgggtcacg gtatgggtat
     6661 cccgtcctgc tccatctaca ccaaagaact gatcaccgat ttcggcgtga agaaaattat
     6721 ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa ctgcgcgacg tcgttatcgg
     6781 tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt tttaaagatc atgactttgc
     6841 cgctatcgct gactttgaca tggtgcgtaa cgcggtagac gcggctaaag cactgggcat
     6901 tgatgctcgc gttggtaacc tgttctccgc tgacctgttc tactctccgg acggcgaaat
     6961 gttcgacgtg atggaaaaat acggcatcct cggcgtggaa atggaagcgg ctggtatcta
     7021 cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc tgcactgtgt ctgaccacat
     7081 ccgcactcac gagcagacca ctgccgctga gcgtcagacc accttcaacg acatgatcaa
     7141 aatcgcactg gaatccgttc tgctgggcga taaagagtaa ttgtgtttcg ctgaaaggcg
     7201 attgtcgtgt taagccggag tgggggactg ctccggcttt ttagtatcta ttca
//
LOCUS       DAZOYS010000194         7231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOYS010000194 DAZOYS010000000
VERSION     DAZOYS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.195"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            389..1426
                     /gene="dusA"
                     /locus_tag="ACHUN9_003552"
     CDS             389..1426
                     /gene="dusA"
                     /locus_tag="ACHUN9_003552"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0696856.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1560..1802
                     /gene="pspG"
                     /locus_tag="ACHUN9_003553"
     CDS             1560..1802
                     /gene="pspG"
                     /locus_tag="ACHUN9_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0696857.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1968..2951)
                     /locus_tag="ACHUN9_003554"
     CDS             complement(1968..2951)
                     /locus_tag="ACHUN9_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0696858.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3034..4449
                     /gene="dnaB"
                     /locus_tag="ACHUN9_003555"
     CDS             3034..4449
                     /gene="dnaB"
                     /locus_tag="ACHUN9_003555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0696859.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4502..5581
                     /gene="alr"
                     /locus_tag="ACHUN9_003556"
     CDS             4502..5581
                     /gene="alr"
                     /locus_tag="ACHUN9_003556"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0696860.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5834..7027
                     /gene="tyrB"
                     /locus_tag="ACHUN9_003557"
     CDS             5834..7027
                     /gene="tyrB"
                     /locus_tag="ACHUN9_003557"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0696861.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1704 a         1771 c         1915 g         1841 t
ORIGIN      
        1 tgttcggggt taaatcggag aaataatatg tgaagttctt cgatagtatg gaaggcatta
       61 tataaaagga cccaatattt attgggttct tttttctcta tcaatgctat tagcagggag
      121 atatatcacc agagtttaat gtgtgatttt ttatttatcg tcgaacctgg attgtttatc
      181 attggcctta acaaagttaa cggctaataa gattatttcc atcacttcgt gagagcttca
      241 tgccttgaga ggatctcaat tttcttttgc aatgagacag gcgcttcctg ttgttatggt
      301 atagtacccc gctattgagc ctcctgaata gtgatgctga ataacataac cccatgatat
      361 atcgataaaa taatctctac atttgaaaat gcacggtaat tctgaaatgc aaaaaatcaa
      421 ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg agtggtcggt ttagcgttgc
      481 accaatgctc gactggacgg acagacattg ccgctatttc ttgcgtctgc tttcccgcaa
      541 tacgttgctg tataccgaaa tggtgaccac aggggcgatt attcacggta aaggtgatta
      601 cctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcggcggta gcgatccggc
      661 ggcgctggca cagtgtgcga agctggcaga agcgcgtgga tatgatgaga tcaacctgaa
      721 tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc tgatgggtaa
      781 tgcgcagctg gttgccgact gcgtgaaagc gatgcgcgat gtggtgtcga ttccagtgac
      841 ggtgaaaacg cgtattggca tcgatgacca ggacagctat gaatttctct gcgatttcat
      901 caacaccgtt tccggcaaag gcgagtgtga gatgtttatc atccacgcac gtaaagcctg
      961 gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt atccgcgtgt
     1021 gtatcaactg aagcgtgact ttccgcatct gacaatgtcg attaacggtg gtatcaagtc
     1081 gctggaagag gccaaagcac acctgcaaca tatggatggc gtgatggtcg ggcgcgaggc
     1141 gtatcagaat ccgggtattc tggcggcggt agaccgggag atctttggtt cctcggatac
     1201 cgatgccgat ccggtggcgg tagtgcgcgc catgtatccg tacattgagc gtgaactcag
     1261 ccaggggacg tatctcggcc atattacccg gcatatgttg ggcttgttcc agggtattcc
     1321 tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg gtgcagacat
     1381 taatgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt tcaccaaaaa
     1441 gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt aaatcaatag
     1501 attatttttg gcatgattct tgtaatgcca gcaagagatt tcatatttgg gagagcatca
     1561 tgctggaact actttttgtg attggctttt ttgtcatgct gatggtcacc ggcgtttcgt
     1621 tgctgggcat tatcgccgcg ctggttgtgg cgacggccat tatgttcctc ggcggtatgc
     1681 tggcattgat gattaagttg ctgccgtggt tactactggc gattgcggtg gtgtgggtta
     1741 ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc tggcgttact
     1801 aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa taggaattga
     1861 taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc ctacatacag
     1921 ccgaactata aaaagaaagg gcttcccagg tggaagccct atttctttta tggaatcaac
     1981 aggctggaac cttgcgtcgc ccggctttcc agaatctcat gcgcacgctg cgcatccttc
     2041 agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc aatcaaagag
     2101 aacagttcat tactggcctc ggttaattcc tcccgcgtgg tgatatagcc ttgcagggaa
     2161 gggcgtgtca catacaacga gcctttttga ttgagaatgc ctaagttcac accggtaacc
     2221 gcacctgatg agttgccaaa actgaccatt aagccgcggc gttgcaggca atccagcgac
     2281 cgttcccagg tgtctctgcc cacggaatcg tacaccacgc gcactttctt accgccggtg
     2341 atctctttta accgctcgac cagatcctct tcacgatagt taataacctg ccacgcgccc
     2401 gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg tgccgataag tttcgcgccc
     2461 agggcttttg cccactggca ggcaattaag ccaacgccgc cagccgctgc gtggaacagg
     2521 aactgttcat cgggtttaat ttcataggtt ttgcgcagca gataataaac cgttaagcct
     2581 ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg caggcagaat cgccgcttta
     2641 tccgcattaa tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc atagactaca
     2701 cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat gcctgccgct
     2761 tcggtaccta atccgctggg tagtgatggc ggcgggtaaa ggccgctgcg gatatacgtg
     2821 tcgataaaat tgatgccgat ggctttattt tcgacctgga tttcattctc tgccggatcg
     2881 gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg aaattcaatt
     2941 cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg gtaaagtttc
     3001 gacccattct ttatctcggt aactccattc actatggcag gaaataaacc cttcaacaaa
     3061 cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt gcctccgcac
     3121 tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga acgctgggac
     3181 gatgtagccg agcgtgtggt ggcagacgat ttttacaccc gcccacaccg ccatatcttt
     3241 actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac ccttgcggaa
     3301 tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct ggcagagctg
     3361 tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg ctgacatcgt gcgtgaacgt
     3421 gccgttgtcc gtgagatgat ctcggttgcg aatgagattg ctgaagccgg ttttgatccg
     3481 caggggcgta ccagcgaaga tctgctggac cttgctgaat cccgcgtctt taaaattgcc
     3541 gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg ccgatgtgct cgacgcaacc
     3601 gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg agtaaacacc
     3661 ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt gatcatcgtc
     3721 gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga acctcgtcga aaacgcggcg
     3781 atgttgcagg ataaaccagt acttatcttc tcgctggaga tgccttcaga acagattatg
     3841 atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac cgggcagctc
     3901 gatgatgaag actgggcgcg catttccggc accatgggta ttttgctcga aaaacgcaat
     3961 atctatatcg atgactcctc cggcctgacg ccaacggaag tgcgttcccg cgcacgccgt
     4021 attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca actgatgcgc
     4081 gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctctcg ctcgctgaaa
     4141 gcactggcga aagaactgaa cgtgccggtg gtggcgctgt cccagttgaa ccgttctctg
     4201 gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg ctctatcgag
     4261 caggatgcgg acttgattat gtttatctat cgtgatgagg tgtatcacga aaacagtgat
     4321 ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat cgggacggta
     4381 cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc gcagtacgac
     4441 gacgaataat aattatttta tgaattaggt aattaaagca aacacttatc aaggaacaca
     4501 aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc tgcaacgtct
     4561 tcgtgaactg gcccctgcca gtaaaatggt tgcggtggtg aaagcgaacg cttatggtca
     4621 cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg tagcccgtct
     4681 cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt tactcgaagg
     4741 cttttttgat gccagagatc tgccgacgat ttctgcgcaa cattttcata ccgccgtgca
     4801 taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg ttaccgtctg
     4861 gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg ctgaggcgtt
     4921 ttatcatcgc ctgacacagt gcaaaaacgt tcgtcagccg gtgaatatcg tcagccattt
     4981 tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa caactcgcta tctttaatac
     5041 cttttgcgaa ggcaaacctg gtcaacgttc cattgccgcg tcgggtggca ttctgctgtg
     5101 gccacaatcg cattttgact gggtgcgccc gggcatcatt ctttatggcg tctcgccgct
     5161 ggaagatcgc tccatcggtg ccgattttgg ctgtcagcca gtgatgtcac taacctccag
     5221 cctgattgcc gtgcgtgagc ataaagccgg agagcctgtt ggttatggtg gaacctgggt
     5281 aagcgaacgt gatacccgtc ttggcgtagt cgcgatgggc tatggcgatg gttatccgcg
     5341 cgccgctcca tccggtacgc cagtgctggt gaacggtcgc gaagtaccga ttgtcggacg
     5401 cgtggcgatg gatatgatct gcgtagactt aggtccacag gcgcaggaca aagccgggga
     5461 tccggtcatt ttatggggcg aaggtttgcc cgtagaacgt atcgctgaaa tgacaaaagt
     5521 gagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat acgtggatta
     5581 atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt atccggccaa
     5641 taaaatccta aaaattcaat aagttgatgt tctttcatgc tcttataaag gtcgtgcctc
     5701 tggcggatgt tcgtttgtca tgagtctcac tctgttgcta attgccgttc gctcctgaac
     5761 atccactcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc ccgtaaacct
     5821 ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga ttcttacgct
     5881 tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagta tcggtctgta
     5941 ctacaacgaa gacggaatta ttccacaact gaaagccgtg gcggaggcgg aagcgcgcct
     6001 gaatgcgcag ccccatggcg cttcgcttta tttaccgatg gaagggctta acagctatcg
     6061 ccatgccatt gcgccgctgc tgtttggtgc ggaccatccg gtactgcaac aacagcgcgt
     6121 agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggcgcgg atttcctgaa
     6181 acgctacttc ccggaatcag gcgtctgggt cagcgatcct acctgggaaa accacgtagc
     6241 aatattcgcc ggggctggat tcgaagtgag tacttacccc tggtatgacg aagcgactaa
     6301 cagcgtgcgc tttaatgacc tgttggcgac gctgaaaaca ttacctgccc gcagtattgt
     6361 gttgctgcat ccatgttgcc acaacccaac gggtgccgat ctcactaatg atcagtggga
     6421 tgcggtgatt gaaattctca aagcccgcga gcttatccca ttcctcgata ttgcctatca
     6481 aggatttagt gccggtatgg aagaggatgc ctacgctatt cgcgccattg ccagcgctgg
     6541 attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg gcgagcgcgt
     6601 cggcggactt tctgttctgt gtgaagatgc cgaagccgct ggccgcgtac tggggcaatt
     6661 gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc aggtggtggc
     6721 tgcagtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag aagagatgcg
     6781 tactcgcatt ctggcaatgc gtcaggaatt ggtgaaggta ttaagcacag agatgccaga
     6841 acgcaatttc gattatctgc ttaatcagcg cggcatgttc agttataccg gtttaagtgc
     6901 cgctcaggtt gaccgactac gtgaagaatt tggtgtctat ctcatcgcca gcggtcgcat
     6961 gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt ttgctgcggt
     7021 gatgtaatgc aggaaagcag gctggagtta cccagcctgc agtgaaatta aactgtcgtc
     7081 gctttcacta tttctttgta gatgattttt ttgatgccgt tgttctacgt gagagataat
     7141 aaaacgttgt cagttctttt attgttaagt ttatcccaat tatctggaat tcctttccct
     7201 attttttcgt gtggagtaat aatttctgca c
//
LOCUS       DAZOYS010000195         7173 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOYS010000195 DAZOYS010000000
VERSION     DAZOYS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.196"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="ACHUN9_003558"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="ACHUN9_003558"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0696862.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2666)
                     /locus_tag="ACHUN9_003559"
     CDS             complement(1671..2666)
                     /locus_tag="ACHUN9_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0696863.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="ACHUN9_003560"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="ACHUN9_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUN9_003561"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHUN9_003561"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0696864.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUN9_003562"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHUN9_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0696865.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..>7173)
                     /gene="emrK"
                     /locus_tag="ACHUN9_003563"
     CDS             complement(6107..>7173)
                     /gene="emrK"
                     /locus_tag="ACHUN9_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0696866.1"
                     /translation="YWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVR
                     QGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLED
                     YNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVV
                     EAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANF
                     KETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIV
                     QRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPELASTVTSMPAYTSKALV
                     IDTSPIEKEISNIISHNGQL"
BASE COUNT         1967 a         1712 c         1663 g         1831 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat agg
//
LOCUS       DAZOYS010000196         7158 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOYS010000196 DAZOYS010000000
VERSION     DAZOYS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.197"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHUN9_003564"
     CDS             <1..488
                     /locus_tag="ACHUN9_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0696867.1"
                     /translation="TLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
                     SLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAILI
                     YALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLD
                     K"
     gene            complement(558..1529)
                     /gene="rfaC"
                     /locus_tag="ACHUN9_003565"
     CDS             complement(558..1529)
                     /gene="rfaC"
                     /locus_tag="ACHUN9_003565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0696868.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(1533..2579)
                     /gene="rfaF"
                     /locus_tag="ACHUN9_003566"
     CDS             complement(1533..2579)
                     /gene="rfaF"
                     /locus_tag="ACHUN9_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0696869.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2589..3521)
                     /gene="rfaD"
                     /locus_tag="ACHUN9_003567"
     CDS             complement(2589..3521)
                     /gene="rfaD"
                     /locus_tag="ACHUN9_003567"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0696870.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3735..4931
                     /gene="kbl"
                     /locus_tag="ACHUN9_003568"
     CDS             3735..4931
                     /gene="kbl"
                     /locus_tag="ACHUN9_003568"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0696871.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4941..5966
                     /gene="tdh"
                     /locus_tag="ACHUN9_003569"
     CDS             4941..5966
                     /gene="tdh"
                     /locus_tag="ACHUN9_003569"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0696872.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6205..7107
                     /locus_tag="ACHUN9_003570"
     CDS             6205..7107
                     /locus_tag="ACHUN9_003570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0696873.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         1755 a         1737 c         1803 g         1863 t
ORIGIN      
        1 atacattgct tatgagaatg aatgacttaa ataacgattt agttaattat tcgcatgata
       61 acaccagaac ttcagtcggt gcccgtctgg caatgtatga agttggctta aaaacatatt
      121 ctccaatagg acaatcactg gaaaaacgag cggaaaaaat acatgaacta gaagaaaaag
      181 agcctagatt gagtggcgct ttaccctata tagattctca tttgcataac gatctcatag
      241 atacgttatc aacgcgtggt attcctggag ttgtattaac aattttagcc ttttcagcaa
      301 tactcatata tgccttaaga actgctaaag aaccttatat tttaatcttg cttttttcac
      361 tactggtagt aggcctaagt gatgtaatac tcttttctaa accggttccg actgctgtgt
      421 ttgtcaccat aatattgctt tgtgcttatt ttaaagcaca atcagaccaa tgtttattag
      481 ataaataatg aatatgtgaa ataaaatcag caaggaggct gattttattt caaaattatc
      541 tattaagggg ttgtaattta aatcatagca gcgttttctt caatgaatcg cttaacagca
      601 tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatctg attcttccca
      661 tatccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg tctatccagt
      721 gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc cccggccagc
      781 acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataagc aaatccttcc
      841 gccagtcgtt tcgcccgctc ttcctcatgc ggcgcgcccc acggaagttt aatccgtatt
      901 cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca gtgtttatca
      961 tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg caggttcgtc
     1021 aggaaatgct gtgcaatagc ataatcgccc tgggtttgcg gtttgctata gcccagactt
     1081 ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat atgatgctta
     1141 cgattgtaaa acagactggc taaaggttcg cgagcggttt gccagtccat gccatgcttt
     1201 acgccatgcg ccagacgcgt caccagcgcc gcgcttttta ccagcccctg agcgtcgata
     1261 actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc cgcttttatg
     1321 ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac tcgctcaacg
     1381 gcggcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc aaacttaatc
     1441 cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac atcgcccatc
     1501 gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa caatagcgcg
     1561 ttgagttctt ccagtacgcg ctggggagta atgtcgatta agctctggtg ataaccctcc
     1621 gcagcgtcac ctttacgcac tttgtgatag ccggtaatca ggcggatcac gcgcgcttta
     1681 tgggatagcg gcggtgtgaa gtccgggcta ctcggaccat acagggcaac cagcggacga
     1741 ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc tttacaggct
     1801 gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg acaccatgcc
     1861 tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc tttcgccgag
     1921 ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc cgcatagtgg
     1981 tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaaacc aatcatcgga
     2041 cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc gctcacctgc
     2101 aattgcggcc ataacaacgg ttttggtaga tcttgtgcgg tacgcatcac gcctttgtca
     2161 taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc gagcacgcgt
     2221 aaatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg aggaataccc
     2281 gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc gcggtcgtag
     2341 cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag cgctccgtga
     2401 ccgagaggca taggaatagc ttcgttaact tccggcatcc gcgataataa tggacggcac
     2461 catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag cgtgcgatag
     2521 agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag tattttcatg
     2581 cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc ttcagcaacg
     2641 gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc ctgagtgaac
     2701 gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg gcctttcttg
     2761 tgataagcca gcgtagcatc agctacagcc tggaaggatt ccgcacgacc agtaccgagg
     2821 ttgaagatgc cggaaacgcc attttccagg aaccacagat tcacatcagc cacgtcgccc
     2881 acatagacga agtcgcgttt gaagttctcg ctaccttcaa acagcttcgg tgattcaccg
     2941 ttgttaagct gggtgttgag atggaaagcg acgctcgcca tgctgccttt atggccttca
     3001 cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttagc ttcaggcagg
     3061 atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt caacggtttt
     3121 tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc tgcggaagag
     3181 gcatacagga acgggatttc gcgctccaga cagtagtgca gcagctcttt ggagtattga
     3241 tagttgttat ccatcatata cttgccatcc cactcggtgg tggaagagca cgcgccttcg
     3301 tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat caggaagtct
     3361 tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt ggtgccgtct
     3421 ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc cttaacgatg
     3481 ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac cttcgaatta
     3541 tggagtcaga gacaatctca gacacgaata cttctatcat atcactacac ggcgttggct
     3601 tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt tgcagaagtg
     3661 ttaacgcgtc atctcgtcgc gacctataag tttgggtaat atgtgctgga atttgccctg
     3721 tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat ctggaaaccg
     3781 cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag caagcagata
     3841 tcactgtggc tgatggaagc cacgtcatta acttttgtgc caataactat ctcgggctgg
     3901 cgaattatcc ggatctgatt gcggcggcaa aggcgggaat ggattctcac ggtttcggca
     3961 tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt gaacaaaaac
     4021 tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt gatgctaacg
     4081 gtggcctgtt tgaaacgctg ctgggcgcgg aagacgccat tatctccgac gcactgaacc
     4141 acgcgtctat tattgatggt gtgcgtctgt gtaaagctaa acgctatcgc tatgccaaca
     4201 acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt gcgcgtcatg
     4261 tgctgattgc caccgatggt gtgttctcga tggacggcgt gattgccaac ctgaagggcg
     4321 tttgcgatct cgcagataaa tatgatgccc tggtgatggt agacgactcc cacgcggtcg
     4381 gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg ggccgggtcg
     4441 atattatcac cggcacgctc ggtaaagcgc tgggcggggc ttctggtggt tataccgcgg
     4501 cgcgcaaaga agtggttgag tggctgcgcc agcgttctcg tccgtacctg ttttccaact
     4561 cgctggcacc ggccattgtt gccgcatcca tcaaagtact ggagatggtc gaagcgggta
     4621 gcgagctgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa atgtcggcgg
     4681 cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt ggtgatgcgg
     4741 tagtggcgca gaaatttgcc cgtgagctgc aaaaagaggg catttacgtc accggtttct
     4801 tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct gcggcgcata
     4861 cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa caactgggcg
     4921 ttattgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa gagggcatct
     4981 ggatgaccga cgttcctgta ccggaactcg ggcataacga tctgctgatt aaaatccgta
     5041 aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg tcgcaaaaaa
     5101 ccatcccggt gccgatggtc gtgggccatg aatatgtcgg tgaagtggta ggtattggtc
     5161 aggaagtgaa gggcttcaag attggcgatc gcgtttctgg cgaaggccat atcacctgcg
     5221 gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg atcggcgtcg
     5281 gcgttaaccg cccgggctgc ttcgccgaat atctggtgat tccggcgttc aacgccttca
     5341 aaattcccga caatatctcc gacgacctgg cttccatttt tgatcccttc ggtaacgccg
     5401 tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct ggtgcaggcc
     5461 cgattggtat tatggcagcg gcggtggcga aacacgttgg tgcacgcaat gtggtgatca
     5521 ctgatgttaa cgaatatcgt ctcgaactgg cgcgcaaaat gggtatcacc cgtgcggtca
     5581 acgtcgctaa agaaaacctt aatgatgtga tggcagaact gggcatgacc gaaggctttg
     5641 atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctc gacaccatga
     5701 accacggcgg tcgcattgcg atgctgggta ttccaccgtc cgatatgtct atcgactgga
     5761 ccaaagtaat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag atgtttgaaa
     5821 cctggtacaa gatggcggcg ctgattcagt caggtctgga tctctcgccg atcattaccc
     5881 atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg ggccagtccg
     5941 ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag cccttttgcc
     6001 tgaggataat ccgttaaata tgtaaaatcc tgtcagtgta ataaagatat cgtaattgcg
     6061 ctgatctctt atatagctgc tctcattatc tctctaccct gaagtgactc tctcacctgt
     6121 aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa acgtcaggat
     6181 aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag tgttattatt
     6241 ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat tacgcaaacc
     6301 tggactgctc tggaaatcat tattattaac gatggttcaa cggataattc tgttgaaata
     6361 gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc gaatgctggc
     6421 gcatcggtgg cgcgtaatcg tgggattgaa gtggcaacgg gcaaatatgt cgcttttgtc
     6481 gatgctgacg atgaagtcta tcccaccatg tacgaaacgc tgatgaccat ggcgttagag
     6541 gacgacctcg acgtggcgca gtgcaacgct gactggtgtt ttcgtgaaac gggagaaacc
     6601 tggaaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg cccggactgg
     6661 ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg ggtttatcgc
     6721 cgcgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca tcaggatatt
     6781 gtctggacaa cagaatttat gtttaatgcg ctgcgtgcgc gatataccga gcaatcgtta
     6841 tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg gaacaaaaac
     6901 cttaattatc aacgtcacta tattaagatt actcgtctgc tagagaaatt aaatcgaaat
     6961 tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta cgaagcattg
     7021 cgtgtttgcc atgcggtgcg caaagagccg gatattatta cccgccaacg gatgattgcc
     7081 gagatattta cttccggtat gtggtaatga ctccaactta ttgatagtgt tttatgttca
     7141 gataatgccc gatgactt
//
LOCUS       DAZOYS010000197         7122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOYS010000197 DAZOYS010000000
VERSION     DAZOYS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.198"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHUN9_003571"
     CDS             <1..436
                     /locus_tag="ACHUN9_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0696874.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHUN9_003572"
     CDS             519..1421
                     /locus_tag="ACHUN9_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0696875.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUN9_003573"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUN9_003573"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0696876.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUN9_003574"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUN9_003574"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0696877.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUN9_003575"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUN9_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0696878.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHUN9_003576"
     CDS             complement(5331..6038)
                     /locus_tag="ACHUN9_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0696879.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUN9_003577"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUN9_003577"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0696880.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHUN9_003578"
     CDS             complement(6896..7099)
                     /locus_tag="ACHUN9_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0696881.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1989 c         1856 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggacgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gtcgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZOYS010000198         7102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOYS010000198 DAZOYS010000000
VERSION     DAZOYS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.199"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..4275)
                     /gene="traI"
                     /locus_tag="ACHUN9_003579"
     CDS             complement(<1..4275)
                     /gene="traI"
                     /locus_tag="ACHUN9_003579"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0696882.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKL"
     gene            4357..4584
                     /gene="vapB"
                     /locus_tag="ACHUN9_003580"
     CDS             4357..4584
                     /gene="vapB"
                     /locus_tag="ACHUN9_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0696883.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4584..4982
                     /locus_tag="ACHUN9_003581"
     CDS             4584..4982
                     /locus_tag="ACHUN9_003581"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0696884.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4991..>7102)
                     /gene="traD"
                     /locus_tag="ACHUN9_003582"
     CDS             complement(4991..>7102)
                     /gene="traD"
                     /locus_tag="ACHUN9_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0696885.1"
                     /translation="RIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYW
                     WCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVA
                     LVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDL
                     PIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSID
                     KILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLM
                     RNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAI
                     RYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGL
                     LAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEK
                     AAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKD
                     MERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMEN
                     RLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPVSPVINDKKSDSG
                     VNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDTWQQMQRR
                     EEVNINVHRERGEDVEPGDDF"
BASE COUNT         1482 a         2194 c         1812 g         1614 t
ORIGIN      
        1 cagtttccgg ttaccttccg tgccttcaag cgcgatggca aaggtttcac tcgccccctg
       61 tgcaccgtgg gcggtgatgg cataggccag gtcaatatgt tgctctgccc gctcctgacc
      121 ggggcgaatc acacgggtct gctgaccgtc agaaagtgtg acactgtcac cggaaaccgc
      181 tgtcaccgtc cagacgctgt tggccacata accgcgctcc cggtcactct tcgtgaagcg
      241 tatcctgtca ccggtcccca cccggatggt gtccggggtg tacagcgtga cgccttcagc
      301 caccgcctcc cggggcgaaa tcagccgcgt gttaccttcc gcatcctgca gggttatcag
      361 cccgtcatcc ttgctgatac cggcaatccg gtgatacaca ttatccacca gggcaagggc
      421 atccgggtta ttctcccagg tggagagacg acgcagctcc ccgtcgcgta tattcgctgt
      481 gttcaggaca ggcaccatga cctgcacctg ccccagttct ccggcctttt cccgtgcatc
      541 atgaatcatg ctgttcagta cgcgccgatc ctcattcagg tgcgtgacaa tcagcgtctg
      601 ctcccgtgct tccggtgtcc tgccggtata gtcgcggaca atggcttcat acagtgtcat
      661 ggggacatcc ggaaaagcct cgcctttcag catcgccttc tgctgcgctt ctgccagttt
      721 cgcttcctgg ctgtgactga actccgtcac ggagtgctcc ggtgcccatg cgccctccag
      781 acgtggcacc tgagacggtt tcacacgctc aagcccggac agcgcccttt ccacatcccg
      841 gttaatcagg ctgtataccg cctcccgcag ttccggcgtc tgacgcacaa tctccttcat
      901 gatgaccaca tcggcagcac tgcgcgtctg ctggagacgg aaaggctgac caggcgcgat
      961 ggcctgcagc tggtccgtgt caccgctggc cacagcccga ccgccaccga ccgcaatcag
     1021 ggcgtatgcc cgtgccatgt cggtattgcc caccattgag ctctcatcga gcaggaacag
     1081 cgtgttgctg aaatccggcg tttctccgct gcgctgctgc agctgcgtgt catgcagaaa
     1141 ggacgccagt gtctgcgcat ccacgccggc gctgcgcatc tccccgaccg cgcggtgcgt
     1201 gggacccagc ccaacgacac ggggacgttc actctccggc agcatgttca cggctgacat
     1261 caccgcccgg aactgtgtgg tcttacccac accggcatag ccctgcacca ccgtgaaacg
     1321 gtcggacgtt tccagtatca tacgggtggc ggcgcgctgt cctgatgtca gtttctccat
     1381 gagttcgcca ggtactctct ccatcagcgg cgtgaccgcc tccttacctt cgagaatatg
     1441 gcgaagaatg ctcttttctg cctcatacga cgcacgggaa accagcaggc ctgtgccata
     1501 gccttttgcc acatccacat acagtaaatc accccgtttt atctgcgcat tgatttcccc
     1561 tcccaggtca gcaaaaccgg ttccttctgc agcaaacgac ttcgcctctg tcagcaggtc
     1621 caccatgctg aaggccaggt ttttactttc cagcacagga agggccagat gaatggcctg
     1681 ctgtgccggc gtgtgaagcc cggctttctg cagactgata gcggtttcca gcaatgtctc
     1741 accggcacgc gcctttatct gctcagaaac caccgtaaag gagggatggc gggcaagttt
     1801 ttccgcagta cgggtttcat ccagtgagga atacagccgg acatcacgac cactgcgggc
     1861 cagaccgttc agggtggcat tgtccattgc catctgtgtg acggaggcaa aaaccgtcgc
     1921 actgtcgctg acggaatgcc cgggcgtttc cacccagccg ttctccagct tcagtgccgt
     1981 gaacggtgaa tcgctcacag gcagggtggc cggttcagcc cgtcccggca caacaaccgt
     2041 catcgcatct tcactgacgg atgccacctg caggcggtca ccgccggaga cgcggagtcc
     2101 gggaattttc cctgtcaccc tcagacgctc accgtctgcc accggcattt tttccggccg
     2161 gaacagcgac cagctgctgt ccagggaaga aatacgcacc acctgcgttt cgccctgcgc
     2221 attccgcagg gtcaggctgt gactctgcgc cgtcacacgt tctgtcacat agcggtcatg
     2281 actgcgtgtc tccgggttcc actgctccat caccattccc gggcggtaca tatcccgcag
     2341 ataacggctc cggctgtcca gccagaccgg tgacagggcg gtcatggtta cctccgggtg
     2401 tccgagcacg ccctgtgttt tcagctcact gcgaatggcc tgtgtcagta tggcctgttc
     2461 ccgtactccg ctgacctgtg ccacgctctc ttctccggct ttcacgctgg ccgcaaaatc
     2521 tccggccagc cgggcatagc ggacattacg gtccggttca ctgatgatgg tggccggtcg
     2581 ctgttctccc ccctgccagc gatatgtgtt caccccggca tccttcatgg ccatcagtgc
     2641 actgcctgta ccggttcgct gcccgctgtc ggttatcagg acctgtacgt tatgacgtgc
     2701 ggcaccatcc agcagggtta acgtctcttt cagggagagt ttttcgccct ggtcgacgat
     2761 aacggtactg cccggcgtga aggccatgcc ttccagcaac tgacgacgtc cggttatcag
     2821 ttcaccggac agccgttcat cctgcttcag gttcatctgc gagcgacggt cagcagcgat
     2881 aatctgcacc tcccgcccct gctcccgggc catcatgacc agttcagcca cccgctcacg
     2941 ctgcccggct gcaccgccct gcccggacac aatggccagc gacggacggt cctgtgccag
     3001 aacactgacg gcatcgctgt aaccggccgt ccgggggaca cttttctccg gatgtacggt
     3061 cacccggttc tgtttcatga tgtcacgact gagtgcccgg actgacagct catcgagcat
     3121 atgaatcccg gacgtgaaca gccccttctc acgatcgagg gggataagct gctcacggct
     3181 gatggcctca tcgataccgg cgcgtgcccg ttcaatcaca ccattttccg gcggcagtat
     3241 gccgaccgtc ctggccagca ggtccgtata cataaactgc actttgcgtt cacttaatcc
     3301 ggcaatggcc tgtgtcaccg cctgctgcac atccggcccg tcctgtgaag cgggtccggg
     3361 tgtctgcgtg cgggtatatg cccgttgttc cgctgcgtca cgatatgccc ggatgtcgaa
     3421 cccggtttcc ttcagcgtct gcatccactc ggccattctg acttcaggat cgacgtgctg
     3481 tttggattta cgcgtatcca gcgccgccac atcccgggat ttcagcgagg cgtcttcccc
     3541 gacggcctcc cggatagtct gtgagcgtcc ggaaaaggcc tccaccggta cacccggcat
     3601 ttcccacata ccgtgcttac cgaccacttc agtttcatag cccagcgcct caacctgctc
     3661 tttccgtttt tcccggtaga gcctgccaaa ggcaatctga ttagcgtaca cattctcaat
     3721 gaaccccgtt ttccccactt tgtcactgct cagtgtcttc cattcgccgt tatgctgcgt
     3781 aacattagtc accaccgcat gcgtgtgtaa ctgtggttcc tgatcgcgac tggtgtcgtg
     3841 gttaaacagt gccatcacca gattaccggt cagcaccgtt tctgactgtc cgtccgtcat
     3901 cacccgtgtg gaggccagtg cctccacctg acggacagca aaatccacgg cctggttatg
     3961 tgcttcaata agacgtttgt cccccccgag catggccatc atggagacac ttttgggagc
     4021 ggagaaggtc aggtcgtagc cgggacgatg cctgttactg ccatcctgca tgcggcttaa
     4081 atccgccccg tccggcagcc tgccctccag aaggcgggta aagacatcct tatcgacact
     4141 gccctgcagc cccagttgtt cagccccctg gccggcccag cgttctccca tgctgcccag
     4201 cacatagtaa ttatccttgt cggtataata attatcggca cttccggccg atctgacctg
     4261 cgcgatactc atcataaacg tatatccctt tgacatatcc cggtatcaat cccacaatag
     4321 atatacacaa gacatatcca cataaggagg caaataatgg aaaccaccgt atttctcagc
     4381 aaccgcagcc aggcggtcag actgccaaaa gcggttgcat tgccggaaaa cgtaaaacgc
     4441 gttgaagtga ttgctgtcgg acgaaccaga atcattacgc cagccggaga gacatgggac
     4501 gaatggttcg acggacacag cgtcagcgcc gattttatgg ataacaggga acagcccggc
     4561 atgcaggaaa gggagtcatt ctgatgctga agtttatgct cgataccaac atctgcattt
     4621 ttacgataaa gaacaaaccc gccagcgtca gggagcgttt taacctgaac caggggagaa
     4681 tgtgtatcag ttcggtcacc ctgatggagc tgatatatgg tgcagaaaaa agccagatgc
     4741 ctgaacgtaa tctcgctgtg atcgagggat ttgtttcccg cattgatgtt ctggattacg
     4801 acgctgctgc agccacacac accggccaga taagagcaga acttgcccgt cagggacgcc
     4861 ctgtcgggcc atttgatcaa atgatcgcag gtcatgcccg cagtcggggg ctgattattg
     4921 tgactaataa cacccgggaa tttgaacgtg tgggcggcct gagaactgaa gactggagct
     4981 gacctgttca tcagaaatca tctcccggct caacatcctc cccgcgttcc cggtgcacat
     5041 tgatgttcac ctcttcacga cgctgcatct gctgccaggt gtccggatga ttttcctgct
     5101 gccatgcctc ataagcggcc atatccacca cttcaccgga ttcactgatc ccgggtggca
     5161 gttgctgttc catctcttct tccggtttca ttttcagctc ctgctcaatc ccccctgccg
     5221 gaacattcac acctgaatct gacttcttat cgttgatgac aggagacacc ggctgttgcg
     5281 gctgttgcgg ctgttcagcc tgagtcacgt cttctccgga aacaacctcc gggacatccg
     5341 gttcgaagag gctggccatc tgacgacctt ctgcttccct tgcggcaagt acggcactca
     5401 gacggttctc catttccggg ttgatgtcac gcggaataaa ctccggagcg accttcggac
     5461 gttcctgata tttcagtgag agtttgactg ccggatacgg tccgggcagg gtgacatagc
     5521 aggtcagatc cggcagggac tgaatgtcgg aataactgac cagcgtctgg cgctccatat
     5581 ccttaccggt cgataccccg tcacgtaccg gatcagcacc gtaggaatac tgttcgctgg
     5641 ctttcaggtg ctctttctca ccaatttcac cgacagcgaa ctccgcaatc ttatggctgg
     5701 gagaacggaa aaaggcacgg gtgttcatga cgtcaaacag cgtggcagcc gctttctcac
     5761 cgtagatatc ttccagctgg gcataggact ggataccaaa cacataacag ccaccgaact
     5821 tacgggcttc cggcaggatc tccaccaggt ccggcagttt gtgtaatgtg ggtaactcat
     5881 cacagaagaa ccacacacga cgattacggt tttctcccat tgccagcaga ccacgaatgg
     5941 caatggacag ccacatggag atcaccggct tcagggaggc atgagtgtcg gcattcgacg
     6001 agataaacag ccagccgttt ttctgatcct cccggacacc acgcatccag tcacggatgg
     6061 tgaagggctc accgttatgc tcaatcccct gcaggtaacg gatggctttc acatagttgg
     6121 tcagcacagc acggatggaa atcgccgttt tctcaatttt ctcttccacc aggttggccg
     6181 ccggtgaatt acgcaggtag gtacgcagtt tttcaatttt gatggaaagc agtgtgtcca
     6241 ccagtttgct gtagctgcgg ttggggtcat tacgcatcag atacgccgct tccgcaaaaa
     6301 tggtacgtcc tgaaccctgc cagaacgggt cctcttttgt gcccatcggg atcagagtat
     6361 ttgcggtatt atcaaaatcc ggctgtgtca ggcactcctt ccacaaatcc caggcggcac
     6421 agcgtgcatc cagcggattc aggatcttat cgatggaggg atcatagtaa cttttaacaa
     6481 attcccctga acggtcataa atcaccacca tatcaccacg ctgacgggcg tagttggcca
     6541 gacgacggat aacctccgac ttaccggccc cgaccgtgcc gtgcagacag aagttctgga
     6601 tttcggaatc ccggataatc ggcagatcgc caatccggat atcggagtcc ctgccgtctt
     6661 ttttcagcat ccgggcaacg tctttcggat tgtctgtcag ttgacgacca cctgtgactt
     6721 cattttcgct ctgttgttta ccctgacgcc ccagaatcca ggagacaaca aagaaggtaa
     6781 tcaggcaaat aaccagtgcc acaactgtgg ccagaacgaa tgcagaccat aactgctcac
     6841 cgcaccagat catatattta tcatgcagta cctgagcagc gttcatccgg aaggttttgc
     6901 cgtaatactg aatctcatat accggctgag acttgattaa atcccgcatg ccttccagcg
     6961 tggtacacca ccagtaaata cagccattca caaacgtctg ccagcttatt tttacccata
     7021 aaaccagacc aacgagtatc cagaaaaaaa taaacaggca gtaaagcata atattggcga
     7081 tctggctgaa catgcggata cg
//
LOCUS       DAZOYS010000199         7022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOYS010000199 DAZOYS010000000
VERSION     DAZOYS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.200"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..232
                     /gene="yafQ"
                     /locus_tag="ACHUN9_003583"
     CDS             <1..232
                     /gene="yafQ"
                     /locus_tag="ACHUN9_003583"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0696886.1"
                     /translation="KLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYR
                     DAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            388..1128
                     /gene="dpaA"
                     /locus_tag="ACHUN9_003584"
     CDS             388..1128
                     /gene="dpaA"
                     /locus_tag="ACHUN9_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0696887.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1099..1866)
                     /locus_tag="ACHUN9_003585"
     CDS             complement(1099..1866)
                     /locus_tag="ACHUN9_003585"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0696888.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SQADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2072..2650)
                     /gene="lpcA"
                     /locus_tag="ACHUN9_003586"
     CDS             complement(2072..2650)
                     /gene="lpcA"
                     /locus_tag="ACHUN9_003586"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0696889.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2890..5334
                     /gene="fadE"
                     /locus_tag="ACHUN9_003587"
     CDS             2890..5334
                     /gene="fadE"
                     /locus_tag="ACHUN9_003587"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0696890.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5377..5823)
                     /gene="ivy"
                     /locus_tag="ACHUN9_003588"
     CDS             complement(5377..5823)
                     /gene="ivy"
                     /locus_tag="ACHUN9_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0696891.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6004..6774
                     /gene="yafV"
                     /locus_tag="ACHUN9_003589"
     CDS             6004..6774
                     /gene="yafV"
                     /locus_tag="ACHUN9_003589"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0696892.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(6816..>7022)
                     /locus_tag="ACHUN9_003590"
     CDS             complement(6816..>7022)
                     /locus_tag="ACHUN9_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0696893.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1638 a         1790 c         1879 g         1715 t
ORIGIN      
        1 aaaacttgca caaaagcgtc ataaggatat gaataaattg aaatatctta tgacgcttct
       61 tatcaataat gctttaccgc ttccagctgt ttataaagac cacccgctgc aaggttcatg
      121 gaaaggttat cgcgatgctc atgtcgaacc ggactggatc ctgatttaca aacttaccga
      181 taaactttta cgatttgaga gaactggaac tcacgcggcg ctctttgggt aaagcacgct
      241 gccgatgaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat
      301 gtgagccttc caaatccaga ctattccgat ttgggatcta ttttgccgtc ctggcgtgtg
      361 taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg
      421 ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg
      481 agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa
      541 gaacgtacgc tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat
      601 aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc
      661 ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa
      721 gcgattaata ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac
      781 ctgatgatcc acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt
      841 gatgagatct tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg
      901 agtatttacc cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt
      961 aaggacttct gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca
     1021 ccgaccgttt ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt
     1081 gtgcagccac aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc
     1141 tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgttgcaa tcaccgtgac
     1201 cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat ccagcagcgt
     1261 tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc aatacgccat
     1321 cacatagcgc ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca gttcatccgc
     1381 ctgtgaggcg atatatttaa acactgccgc catgttgccc ggtgtgcgcg ggtaacgctg
     1441 cgttaattta tgcaggagcc agcagaaggc tttttcgcta tcggtttcac cgaccgggcg
     1501 gaagttgccg gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca
     1561 gttacgcccc cataactcac gggtaaacgg gtgagtattt tccagcgcca cctcgccccg
     1621 attggcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag
     1681 tttggcgatg ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc
     1741 ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgcac
     1801 cagaccggtg aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc
     1861 gcacatgggt aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag
     1921 aattttcagg ccggatgcgg cgtaaacgtc ttattcggcc tacggttcgc gtacagattt
     1981 gtaggcatga taagacgcat cagcgtcgca tcaggcatca gcgcacaagt gccggatgcg
     2041 gcgtaaacgc cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact
     2101 ggatcaggat atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt
     2161 gcggaacgcg aatttcgata tccgccgtgc cagccatttt accgccgtct ttaccggtca
     2221 gggtgatcac tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag
     2281 agttaccgga ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt
     2341 agcgggagaa aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt
     2401 cagaaatagc aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt
     2461 cggcaaagtg catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac
     2521 cggctttaaa gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt
     2581 catcttttaa aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat
     2641 cctggtacat gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat
     2701 accgccaaaa gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc
     2761 ggataaaaac agcaacaatg tgagctttgt tgtaattata ttgtaaacac attgctaaat
     2821 gtttttacat ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg
     2881 cttttcgtta tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc
     2941 tatcaccgcg tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc
     3001 ggcgttgctg gtctgtggtc agcgtgggta ctggtgcctc tggccattat cctcgtgcca
     3061 tttaactttg cgcctatgcg taagtcgatg atttccgcgc cggtatttcg cggtttccgt
     3121 aaggtgatgc cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg
     3181 tgggagggcg acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag
     3241 ccgcgcctga ccgccgaaga gcaagcgttt ctcgacggcc cggtagaaga agcctgccgg
     3301 atggcgaatg atttccagat tactcatgag ctggcggatc tgccgccgga gttgtgggcg
     3361 taccttaaag agcatcgctt cttcgcgatg attatcaaaa aagagtacgg cggactggag
     3421 ttctcagctt atgcccagtc tcgcgtgctg caaaaactct ccggcgtgag cgggatcctg
     3481 gcgattaccg tcggcgtgcc gaactcatta ggcccgggcg agctgctgca acattacggc
     3541 actgacgagc agaaagatca ctatctgccg cgtctggcgc gtggtcagga gatcccttgc
     3601 tttgcactga caagcccgga agcgggttcc gatgcgggcg cgattccgga caccgggatt
     3661 gtctgcatgg gcgaatggca gggccagcag gtgctgggga tgcgtctgac ctggaacaaa
     3721 cgctatatta cgctggcacc gattgcgact gtgcttgggc tggcgtttaa actctccgac
     3781 ccagaaaagc tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct gatcccgacc
     3841 accacgccgg gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtacc gttccagaac
     3901 ggaccgacgc gcggtaaaga tgtcttcgta ccgatcgatt acatcatcgg tgggccgaaa
     3961 atggccgggc aaggctggcg gatgctggtg gagtgcctct cggtaggccg cggcatcacc
     4021 ctgccttcca actcaaccgg cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct
     4081 cacattcgcc gtcagttcaa aatctctatc ggtaagatgg aagggattga agagccgctg
     4141 gcgcgtattg ccggtaatgc ctacgtgatg gatgctgcgg catcgctgat tacctacggc
     4201 attatgctcg gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca ctgtacccac
     4261 cgcgggcagc agtcgattat tgatgcgatg gatatcactg gtggcaaagg cattatgctc
     4321 gggcaaagca acttcctggc gcgtgcttac cagggcgcac cgattgccat caccgttgaa
     4381 ggggctaaca ttctgacccg cagcatgatg atcttcggac aaggagcgat tcgttgccat
     4441 ccgtacgtgc tggaagagat ggaagcggcg aagaacaatg acgtcaacgc gttcgataaa
     4501 ctgttgttca aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc
     4561 ctgacgcgcg gtttaaccag cagcacgcca accggcgatg ccactaaacg ctactatcag
     4621 cacctgaacc gcctgagcgc caacctcgcc ctgctttctg atgtctcgat ggcagtgctg
     4681 gacggcagcc tgaaacgtcg cgagcgcatc tcggcccgtc tgggggatat tttaagccag
     4741 ctctacctcg cctctgccgt gctgaagcgt tatgacgacg aaggccgtaa tgaagccgac
     4801 ctgccgctgg tgcactgggg cgtacaagat gcgctgtatc aggctgaaca ggcgatggat
     4861 gatttactgc aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc
     4921 ccgaccggac gtcattatct ggcaccttct gacaagctgg atcataaagt ggcgaagatt
     4981 ttacaagtgc cgaacgccac ccgttcccgc attggtcgcg gtcagtacct gacgccgagc
     5041 gagcataatc cggttggctt gctggaagag gcgctggtgg atgtgattgc cgccgaccca
     5101 attcatcagc ggatctgtaa agagctgggt aaaaacctgc cgtttacccg tctggatgaa
     5161 ctggcgcaca acgcgctggc gaaggggctg attgataaag atgaagccgc tattctggtg
     5221 aaagctgaag aaagccgtct gcgcagtatt aacgttgatg actttgatcc ggaagagctg
     5281 gcgacgaagc cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc gtaattgaat
     5341 aacggagccg aaaggctccg tttctttatc cgctaattat ttaaaattaa agccatccgg
     5401 atggttttcc aggctgccgg tcaacgccgc gaacagcacc gttttaccat caatcgaaag
     5461 cgcatcgttc acattcagcc aggtgagttt ctcttgcgac gttttctcat caatagtcga
     5521 gaacagcccc gtcatctgat tagatttctc ggaccacatc acagcgatac gttgcgagcc
     5581 acagtcatgc ggtttgcacg cgctcatcac ctgatacgta tcatctccca acgttacggt
     5641 ttgtgcggga gtataagtac cgcctttcat cacccaggca ggcagcttat gcccttgtac
     5701 catctgatta aatgcagctt tggtggtttc gccctttgca aggctgctaa tggttaaatc
     5761 atcctgcgcc attgcactgg tggcgatgac cagagcggcg actgtcgtta ttgtcttaaa
     5821 catcattcct cccgagctta tcctgcccat gttattaacc ttctaaagcc tggcgataac
     5881 acgataattt gcaaatcaga tatttccaat atcagcattt gttatcaatt tattctgcgc
     5941 taaggcaact gcctgataag gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac
     6001 accgtgcctg gtttgaagat tacgcttttg cagcaaccac tggtgtggat ggatggccct
     6061 gccaacctgc gtcattttga tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt
     6121 ctaccgaaga tgtttaccag cggctttgcc atggaagcgg cagcttcgtc gctagcacaa
     6181 gatgacgtag tggactggat gacagctaag gcgcagcagt gcaatgcgct gattgcaggc
     6241 agtgttgcat tacaaacgga gtctggttcg gttaaccgct ttttgctggt tgagccgggc
     6301 ggcacggtac atttttatga taagcgtcat ctgttccgca tggcagatga gcatctacat
     6361 tataaagcgg gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat tttgccgttg
     6421 gtgtgctacg acttacgttt tccggtgtgg tcgcgcaatc tcaacgatta tgacctcgcc
     6481 atctacgttg ccaactggcc tgctccgcgc tctctgcact ggcaggcatt gctgacggcc
     6541 cgcgcgattg agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac
     6601 ggctgccatt atcgcggtga cagccgggtg attaatccac aaggcgagat tatcgctact
     6661 gccgacgcgc atcaggcaac gcgcattgat gcggagctgt cgatgatggc attgcgggaa
     6721 tatagagaaa agtttccggc atggcaggat gcggatgagt ttaggttgcg gtgagtgggg
     6781 gttgtgttta aggctggggg agagtcgggg taagactacg aaagcccgct ctccgcaagg
     6841 actgacgcca gatagtttct gtccatagct gcttttcgca tcttacgtct taaccctgcc
     6901 ttgaatacct tatcattcgt caaaatatta atagcgatgt gccgtatccc tgaaaataat
     6961 tctgctgcat ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc
     7021 ca
//
LOCUS       DAZOYS010000200         6968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOYS010000200 DAZOYS010000000
VERSION     DAZOYS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.201"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            502..1491
                     /gene="araF"
                     /locus_tag="ACHUN9_003591"
     CDS             502..1491
                     /gene="araF"
                     /locus_tag="ACHUN9_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0696894.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1561..3075
                     /gene="araG"
                     /locus_tag="ACHUN9_003592"
     CDS             1561..3075
                     /gene="araG"
                     /locus_tag="ACHUN9_003592"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0696895.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3090..4076
                     /gene="araH"
                     /locus_tag="ACHUN9_003593"
     CDS             3090..4076
                     /gene="araH"
                     /locus_tag="ACHUN9_003593"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0696896.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4243..5043
                     /gene="otsB"
                     /locus_tag="ACHUN9_003594"
     CDS             4243..5043
                     /gene="otsB"
                     /locus_tag="ACHUN9_003594"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0696897.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5018..6442
                     /gene="otsA"
                     /locus_tag="ACHUN9_003595"
     CDS             5018..6442
                     /gene="otsA"
                     /locus_tag="ACHUN9_003595"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0696898.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6449..6877)
                     /gene="uspC"
                     /locus_tag="ACHUN9_003596"
     CDS             complement(6449..6877)
                     /gene="uspC"
                     /locus_tag="ACHUN9_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0696899.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1757 a         1627 c         1813 g         1771 t
ORIGIN      
        1 tgattttata aaacgttttt tcatttttgc gagatggctc tcattatacg tgttctgtta
       61 attaatgagc acagtgataa ttaatggcga aatgaatgac gtgcattttc cacatctttg
      121 agttgcggtt attacaccat ttcaaaaact caacgccagg taatgcggcc tattgactgg
      181 ttaaaaagaa gacatcccgc atgggtacca aagacaacaa ggatttccag gctaatctta
      241 tggattaatc tgctgtgcat tcgacaattt gtctgacaaa ttggctttcc cttatgtctt
      301 ttcccgctaa atttatgcac gttctcactg taattctgcg atgtgatatt gctctcctat
      361 ggagaattaa tttctcgcta aaactatgtc aacacggtca cttatctttt agataaaagg
      421 taatgctttg ttttccgatt aatttaacga aagtcattcg tttttgccct acacaaaacg
      481 acactaaagc tggagagaac catgcacaaa tttactaaag ccctggcagc cattggtctg
      541 gcagccgtta tgtcacaatc cgctatggcg gagaacctga agctcggttt tctggtgaag
      601 caaccggaag agccgtggtt ccagaccgaa tggaagtttg ccgataaagc cgggaaggat
      661 ttagggtttg aggttattaa gattgccgtg ccggatggcg aaaaaacatt gaacgcgatc
      721 gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt gtactccgga ccccaaactc
      781 ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga aagtcattgc cgtggatgac
      841 cagtttgtta acgccaaagg taagccaatg gataccgttc cgctggtgat gatggcggcg
      901 actaaaattg gcgaacgtca gggtcaggaa ctgtataaag agatgcagaa acgtggctgg
      961 gatgtcaaag aaagcgcggt gatggcgatt accgccaacg aactggatac ggcacgtcgc
     1021 cgtacaacgg gttctatgga tgcgctgaaa gcggccggat tcccggaaaa acaaatttat
     1081 caggtaccta ccaaatctaa cgacatcccg ggggcatttg acgctgccaa ctcaatgctg
     1141 gttcaacatc cggaagttaa acattggctg atcgtcggta tgaacgacag caccgtgctg
     1201 ggcggcgtac gcgcgacgga aggtcagggc tttaaagctg ccgatatcat cggcattggc
     1261 attaacggtg tggatgcgtt gagcgaactg tctaaagcac aggcaaccgg cttctacggt
     1321 tccctgctgc caagcccgga cgtacatggc tataaatcca gcgaaatgct ttacaactgg
     1381 gtagcaaaag acgttgaacc gccaaaattt accgaagtta ccgacgtggt gctgatcacg
     1441 cgtgacaact ttaaagaaga actggagaaa aaaggtttag gcggtaagta atttgccgga
     1501 aaaattcccc tctgcatgat gcagaggggg tgtgaacgac cagtgattca cggagacgtt
     1561 atgcaacagt ctaccccgta tctctcattt cgcggcatcg gtaaaacgtt tcccggcgtt
     1621 aaggcgctga cggatattag ttttgactgc tatgccggtc aggttcatgc gttgatgggt
     1681 gaaaatggcg caggaaaatc aactctctta aaaatcctca gcggcaacta tgcgccaacc
     1741 acgggttctg tagtgattaa tgggcaggaa atgtcctttt ccgacacgac cgcagcactt
     1801 aacgcgggtg tggcgattat ttaccaggaa ctgcatctcg tgccggaaat gaccgtcgcg
     1861 gaaaacatct atctcggcca gctgccgcat aaaggcggca ttgtgaatcg ctcattgctg
     1921 aattatgagg cgggtttaca acttaaacat cttggtatgg atattgaccc ggacacgccg
     1981 ctgaaatatc tctccattgg tcagtggcag atggttgaaa tcgccaaggc gctggcgcgt
     2041 aacgctaaaa ttatcgcctt tgatgagcca accagctccc tttctgcccg cgaaatcgac
     2101 aatcttttcc gcgttattcg tgaactgcga aaagaggggc gggtgatctt atacgtttct
     2161 caccgtatgg aagaaatatt tgccctcagc gatgccatca ctgtctttaa agatggacgt
     2221 tatgtcaaaa cctttaccga tatgcagcag gttgaccacg acgcgctggt gcaggcgatg
     2281 gtcgggcgcg acattggcga tatctacggc tggcaacctc gtagttatgg cgaggagcgc
     2341 ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc caataagtct ggcggttcgc
     2401 agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg ggcgtagcga attaatgaaa
     2461 ggcttgtttg gcgggacgca aatcaccgcc ggtcaggttt atatcgacca acagccgatc
     2521 gatattcgta aaccgagcca cgccattgcc gcaggcatga tgctctgccc ggaagatcgc
     2581 aaagcggaag gcattattcc cgtgcactcc gttcgcgaca atatcaacat cagtgccaga
     2641 cgcaaacatg tgctcggcgg ttgtgtaatc aacaacggtt gggaagaaaa caatgccgat
     2701 cagcacattc gttcgctcaa catcaaaacg ccgggcgctg agcaactgat catgaatctc
     2761 tcaggcggaa atcagcaaaa agccattctg ggccgctggt tatcggaaga gatgaaggtc
     2821 attttgctgg atgaacctac gcgcagcatt gatgttggtg ctaagcacga aatatataac
     2881 gtaatttatg cgctggcggc gcagggtgtg gcggtgctgt ttgcctccag cgacttacct
     2941 gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg aaggtgaaat cgccggtgaa
     3001 ttgttacacg agcaggcaga tgagcgtcag gcactgagcc ttgcgatgcc taaagtcagc
     3061 caggcagttg cctgagtaag gagagtatga tgtcttctgt ttctacatcg ggttctggcg
     3121 cacctaagtc gtcattcagc ttcgggcgta tttgggatca gtacggcatg ctggtggtgt
     3181 ttgcggtgct ctttatcgcc tgtgccattt ttgtcccaaa ttttgccacc ttcattaata
     3241 tgaaagggtt gggcctggca atttccatgt cggggatggt ggcgtgtggc atgttgttct
     3301 gccttgcttc cggtgacttt gacctttctg tcgcctccgt aattgcctgt gcgggtgtca
     3361 ccacggcggt ggttatcaac ctgactgaaa gcctgtggat tggcgtggca gcggggttgc
     3421 tgcttggcat tctctgtggc ctggtcaatg gctttgttat cgccaaactg aaaataaatg
     3481 ctctgatcac aacactggca acgatgcaga ttgttcgagg tctggcgtac atcatttcag
     3541 acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc ccttggttac gctaactggt
     3601 tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct gattatcttt ggtttgttgc
     3661 tgaataaaac cacctttggt cgtaacaccc tggcgattgg cgggaacgaa gaggctgcgc
     3721 gtctggcggg tgtaccggtt gttcgcacca aaattattat ctttgttctc tctggcctgg
     3781 tatctgcgat agccggaatt attctggctt cacgtatgac tagtgggcag ccaatgacgt
     3841 cgattggtta tgagcttatt gttatctccg cctgcgtttt aggtggcgtt tctctgaaag
     3901 gtggcatcgg aaaaatctca tatgtggtgg cgggtatctt aattttaggc accgtggaaa
     3961 acgccatgaa cctgcttaat atttctcctt tcgcgcagta cgtggttcgc ggcttaatcc
     4021 tgctggcagc ggtgatcttc gaccgttaca agcaaaaagc gaaacgcact gtctgatgct
     4081 tttttccaca acaatttaac gttttttccc accacagcca gccgccacaa cggttggctg
     4141 ttcttcattg caaatggcga cccccgtcac actgtctata cttacatgtc tgtaaagcgc
     4201 gttctgcgca aaacaataag aaaagagaag gaggagagcc gggtgacaga accgttaacc
     4261 gaaacccctg aactatccgc gaaatatgcc tggttttttg atcttgatgg aacgctggcg
     4321 gaaatcaaac cgcatcccga tcaggtcgtc gtgcctgaca atattctgca aggactacag
     4381 ctactggcaa ccgcaagtga tggtgcattg gcattgatat cagggcgctc aatggtggag
     4441 cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg gcgtgcatgg ggcggagcgc
     4501 cgtgacatca atggtaaaac acatatcgtt catctgccgg atgcgattgc gcgtgatatt
     4561 agcgtgcaac tgcatacagt catcgctcag tatcccggcg cggagctgga ggcgaaaggg
     4621 atggcttttg cgctgcatta tcgtcaggct ccgcagcatg aagacgcatt aatgacatta
     4681 gcgcaacgta ttactcagat ctggccacaa atggcgttac agcagggaaa gtgtgttgtc
     4741 gagatcaaac cgagaggtac cagtaaaggt gaggcaattg cagcttttat gcaggaagct
     4801 ccctttatcg ggcgaacgcc cgtatttctg ggcgatgatt taaccgatga atctggcttc
     4861 gcagtcgtta accgactggg cggaatgtca gtaaaaattg gcacaggtgc aactcaggca
     4921 tcatggcgac tggcgggtgt gccggatgtc tggagctggc ttgaaatgat aaccaccgca
     4981 ttacaacaaa aaagagaaaa taacaggagt gatgactatg agtcgtttag tcgtagtatc
     5041 taaccggatt gcaccaccag acgagcacgc cgccagtgcc ggtggccttg ccgttggcat
     5101 actgggggca ctgaaagccg caggcggact gtggtttggc tggagtggtg aaacagggaa
     5161 tgaggatcag ccgctaaaaa aggtgaaaaa aggtaacatt acgtgggcct cttttaacct
     5221 cagcgaacag gaccttgacg aatactacaa ccaattctcc aatgccgttc tctggcccgc
     5281 ttttcattat cggctcgatc tggtgcaatt tcagcgtcct gcctgggacg gctatctacg
     5341 cgtaaatgcg ttgctggcag ataaattact gccgctgttg caagacgatg acattatctg
     5401 gatccacgat tatcacctgt tgccatttgc gcatgaatta cgcaaacggg gagtgaataa
     5461 tcgcattggt ttctttctgc atattccttt cccgacaccg gaaatcttca acgcgctgcc
     5521 gacatatgac accttgcttg aacagctttg tgattatgat ttgctgggtt tccagacaga
     5581 aaacgatcgt ctggcgttcc tggattgtct ttctaacctg acccgcgtca cgacacgtag
     5641 cgcaaaaagc catacagcct ggggcaaagc atttcgaaca gaagtctacc cgatcggcat
     5701 tgaaccgaaa gaaatagcca aacaggctgc cgggccactg ccgccaaaac tggcgcaact
     5761 taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc gaacggctgg attattccaa
     5821 aggtttgcca gagcgttttc tcgcctatga agcgttgctg gaaaaatatc cgcagcatca
     5881 tggtaaaatt cgttataccc agattgcacc aacgtcgcgt ggtgatgtgc aagcctatca
     5941 ggatattcgt catcagctcg aaaatgaagc tggacgaatt aatggtaaat acgggcaatt
     6001 aggctggacg ccgctttatt atttgaatca gcattttgac cgtaaattac tgatgaaaat
     6061 attccgctac tctgacgtgg gcttagtgac gccactgcgt gacgggatga acctggtagc
     6121 aaaagagtat gttgctgctc aggacccagc caacccgggc gttcttgttc tttcgcaatt
     6181 tgcgggagcg gcaaacgagt taacgtcggc gttaattgtt aacccctacg atcgtgacga
     6241 agttgcagct gcgctggatc gtgcattgac tatgtcgctg gcggaacgta tttcccgtca
     6301 tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac cactggcagg agtgcttcat
     6361 tagcgaccta aagcagatag ttccgcgaag cgcggaaagc cagcagcgcg ataaagttgc
     6421 tacctttcca aagcttgcgt aggagcggtt aatctcccgt aagtggaact aacagcacat
     6481 cgacctcact tgaggcaatc actcttttcg ccgagcagga tgctcgcgaa aagaaactgt
     6541 gattgtgatt accgcaaatc accaaatcga aatgatgttt gtgacatact tccagaatat
     6601 gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac gggatacccc gcatcctgaa
     6661 ttaacttatc aagaaagctt tgcgtttctt cttgcatcac gctgcgtaaa tcttcaagca
     6721 tcggcgcggc taattgattg tacatttccg ggtcggaagc gagagtaatt aaactgatgt
     6781 gtccctttac tggcctggcg atagataccg cttttgccag cagttgctga ctttccggtg
     6841 taaccgcaac agcgacaaga atattgctat agctcataac ctgttcctta ttctgtgaac
     6901 ttcaggtgac attaaagctt tactcctaat ggagaaacga cgcaatccca actcggtcaa
     6961 acaggtaa
//
LOCUS       DAZOYS010000201         6911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOYS010000201 DAZOYS010000000
VERSION     DAZOYS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.202"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /gene="cas6e"
                     /locus_tag="ACHUN9_003597"
     CDS             complement(<1..215)
                     /gene="cas6e"
                     /locus_tag="ACHUN9_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0696900.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(140..886)
                     /gene="cas5e"
                     /locus_tag="ACHUN9_003598"
     CDS             complement(140..886)
                     /gene="cas5e"
                     /locus_tag="ACHUN9_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0696901.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="ACHUN9_003599"
     CDS             complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="ACHUN9_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0696902.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1964..2500)
                     /gene="casB"
                     /locus_tag="ACHUN9_003600"
                     /gene_synonym="cse2"
     CDS             complement(1964..2500)
                     /gene="casB"
                     /locus_tag="ACHUN9_003600"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0696903.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2497..4059)
                     /gene="casA"
                     /locus_tag="ACHUN9_003601"
                     /gene_synonym="cse1"
     CDS             complement(2497..4059)
                     /gene="casA"
                     /locus_tag="ACHUN9_003601"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR0696904.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4157..6856)
                     /locus_tag="ACHUN9_003602"
     CDS             complement(4157..6856)
                     /locus_tag="ACHUN9_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFR0696905.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1685 a         1867 c         1627 g         1732 t
ORIGIN      
        1 tggagttctt cccgacgata aagaaattgc ctttctttgc cgccagggaa aagatcccac
       61 agccactgat gcatcacata ttcaccgcga tccaccagat gcagcaactg cgcaggcgaa
      121 agctggccgg tatgtaaggt tattcttgag aggtacatgg ttcctccttg ctgagccacg
      181 gcccctgatt gacggtgcgt tccccaaaca gccactgctg acgatttaaa ggaacatccc
      241 ggcggcgtaa tattttgctc gcgaccaggc cgtcgtgttt cccttcccac cagcattcat
      301 cctgaagttt cgggagtgag actttcagtt tatggaaatg atcctgatac tgttggtatg
      361 cattacataa ggcatcacac gcgttgcctt cgagcagtaa cggcgcaaga ggtaatgcca
      421 gcggatgact ttttcgcccc agataaagcg gaaaaactgg atggcgtaaa ccgtcctgca
      481 actgttcgag gctgtaaggc gcatcggctg ttgttgccac cgccaccatc caccaggcat
      541 cggtgtagta gtcgcgccgg gagataatcg cgctcaaaag ctcagggtcg ctcaactctt
      601 cacgacggct gaaataacgt gctttacgca cctcttttgg catctggacc gtgtgataat
      661 cccgtgccca gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg cgattaaacg
      721 cgtttaagcg ttcggtatca tcacgccgaa tccctacccc ggcagccagt agccccagca
      781 atgctgagcg cgaaggaagt tcatgggtat gacgaacttc acctggagca tcgacacccc
      841 aggacgccat cggcccatga agctgaaaaa tcagatattg gctcataagc cgccccttac
      901 gcgcagataa agtccagcac gtccttcatg cttccctgtt tgttcatcac gtcaaaactt
      961 gcgcattcgg tcttctgttc gtagaccgta ttcatgtttt cgcgaagcgt tgtaatacgc
     1021 tgcaccgcca catctaactg acgtgtgcca ttaatgggtt catagaaagc cgccgccaga
     1081 gagcgtggtt gatcggtgcc tttttctgcc agcgcccagg aagcataggc acggctggca
     1141 aagctatttt gtttaccggt tggggaaact ttaagcgcgg cttccgtaaa agcgcgtatg
     1201 gtctgattag ctaacgcttc gtcaccgccg aggttttcta ccagcagatc tttatcaatg
     1261 cagatatagg tgtagaaaag tgctgaaccg aatccggttt ctccgagatg cccggcaccg
     1321 gcatcttcag aggcctggcg caaatcatca acggcggtga aaaaatcatc ttcgacaatc
     1381 gtttcactga caccaaacgc atgcgcgacc tggcaggcgg cttcaacatt aaactcaggt
     1441 ttattcgcca gcatacgacc aaacatagcg atatctaccg ctatacggtc tttacgtaac
     1501 aacgcgagat cttcctcttt tggcgcgcgc ttttcttctg ccagttgatg agcaagcgct
     1561 tttacggcgt caaattctgc cgggctgata tgaactaatt gttcagtttc ggcgttagtg
     1621 agcggatctt ttggtttttt gtcattttta gctttcccaa gataatcagc aatttttgcc
     1681 gcccattcga tggctttttt atcttcaatc cctttctcaa tcaggatagt tgccgcctca
     1741 cgcgcaatac gcccactgcg aataccaata tggcccgcca gtgcctgttc aaaaagtgca
     1801 gaagtgcgcc acgcacgttt cagactttgc gaggaaacgc gcagacgcgt tgctccaccc
     1861 agcaccacag tttttggcgc accggtgtca tcacggttaa ggttggcagc agggtaagcg
     1921 gttaacacat gaagctgaat aaatgttgtc atgagatagt cctttattga ttttgttcgt
     1981 tatcggcgtc gccagcctgg taatattcca gcgcccagcg gatacggata aattcggttg
     2041 gccgctgctg gcggcggtga tgattgagca ggtcgtcgct ctcctggcac cagcggaaaa
     2101 tgccatctgc cagggagtca agattgacgg aaccgtttaa taatctgacg gcgcggcgta
     2161 actggcgcag taattcatcc ggtgttttta ctgctgacag gcgagtaaaa cgcccctttg
     2221 acattaccgc cgccagttgc gcggcaaaag gctgccgttc atcaatggct ttaacattgg
     2281 cgctaactgc ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact
     2341 ttatttgtct gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg
     2401 tcgtactacg ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt
     2461 tgcgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt
     2521 ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc
     2581 ggtcatgatg cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa
     2641 atcctgacgc gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc
     2701 ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa
     2761 gtcgatatcc acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc
     2821 tttcaatgca ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag
     2881 tttcggtatc tggccttctt ttttattgag cagcagcggg aaatgatgtt cgtaccaaca
     2941 gcgcgctttc atgttgtcga aatcatagcc aaatccccac aggcccactt ttgcctgctt
     3001 gagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga
     3061 cttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt
     3121 gaccgagtaa aactcgccgc cctctttaag tagtacacgg taaggcgtta agggatgctg
     3181 ccacatggca tagttagcgc cgtaattttt ggtcgtcatc aggctcagaa gcgcgtcgct
     3241 ctgctcaccg caaatatcgc agttgccgac tgtggtggta ttaaaatcaa tacgaatacg
     3301 gcgaggcatt ccccagtacg cctggagttt attgacctgc tcatcggtta ccaccgcacc
     3361 ggccagttcg ctgatgcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt
     3421 gggtagcggt aaatcggctt catcctgcgg catgacattg atccacagtt tgcgccacaa
     3481 gggagtttgt tgattaccct gatattcctg taattcaatc agagtggtca tcggcccacc
     3541 gccgcgtaaa ccggtgcggt agcctttgcc acctgacggc gcattcaact gtagggagaa
     3601 cagagctaac gcagagcaat gtgggcacag gtattccgtt acaccacgct tgataaagtg
     3661 gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgaccgg
     3721 aactttatct ccggtgagcg cctcgaaatc ctgtataaat gaaggggaat ctggcccaaa
     3781 ctggaaagca tgttctaatg acagcaatgc ttcccgtagc ttttcagctt ccagtccgtc
     3841 ttcccagata tcatcccaac gacgatgatc ttttggcgcg aagctggttt gtagtagccc
     3901 cagcaaaaac tgccataccg caccctggag atctgcccgc ggcgcagaaa tatcgacaac
     3961 attttcatcc gccagatcga ctggcgccag tttgcctgtc gttccgtctt taaaacgaac
     4021 gggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag
     4081 accatcccag gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag
     4141 tcctgtcgca gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccgc
     4201 aactttcgtc atcgttgact aaaataacgt ttgccatctc cggatcttgt cgctgttcaa
     4261 tgcaccacag cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc
     4321 accagcttcg gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag
     4381 gcttcaccac accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac
     4441 gtgtcgacag tttttctggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt
     4501 tcagcacaaa ctttttagcc attgcgcgat cacagtaata tttgcccact tgctcctgct
     4561 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa
     4621 gccgggctgc gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc
     4681 agattcgccc atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt
     4741 cgtcgccagg agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct
     4801 cgtcctttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc
     4861 ctgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac
     4921 aatcgacact ctgctctaat acctgagtac aaataaggac ttttccggca cgctgtgagc
     4981 tatcttcttt accaaagcgt gccagtgtct ccgtttcaat tcgctggcga tcgctaaaag
     5041 caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct
     5101 gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
     5161 gacttaccgc cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc
     5221 tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg
     5281 tgacatgcgt cagccagggg taatcatcct tttccaggaa cggagcttct tgcaggccct
     5341 ctgtaccccg cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag
     5401 aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc
     5461 acgacatgta agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc
     5521 ccagaagccg cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg
     5581 tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac
     5641 atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga
     5701 gtgattcatt aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc
     5761 gggattctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg
     5821 ccgttgccat tgttggcaga ccaaaaaaca gcccctgagc cttccccgca gctatcaacc
     5881 tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa
     5941 ataactgcgg tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
     6001 gattctcaat aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta
     6061 gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa
     6121 tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga
     6181 gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat
     6241 catcccagaa tgcggggatc tctatgagcg gaaacagtgg tttgatttca aggagaaaat
     6301 ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg
     6361 gccgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc
     6421 gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca
     6481 accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa
     6541 tcttgagttc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt
     6601 gccagcacag caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt
     6661 gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg
     6721 ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc
     6781 gtcgggtttt tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta
     6841 aaggatattt acgcatatat taaaaattat ctgtgatata tatcattcaa taaaatattt
     6901 acattatgaa t
//
LOCUS       DAZOYS010000202         6883 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOYS010000202 DAZOYS010000000
VERSION     DAZOYS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.203"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..186
                     /gene="ymgJ"
                     /locus_tag="ACHUN9_003603"
     CDS             1..186
                     /gene="ymgJ"
                     /locus_tag="ACHUN9_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0696906.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            147..266
                     /locus_tag="ACHUN9_003604"
     CDS             147..266
                     /locus_tag="ACHUN9_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0696907.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(745..1011)
                     /gene="minE"
                     /locus_tag="ACHUN9_003605"
     CDS             complement(745..1011)
                     /gene="minE"
                     /locus_tag="ACHUN9_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0696908.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1015..1827)
                     /gene="minD"
                     /locus_tag="ACHUN9_003606"
     CDS             complement(1015..1827)
                     /gene="minD"
                     /locus_tag="ACHUN9_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0696909.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1851..2546)
                     /gene="minC"
                     /locus_tag="ACHUN9_003607"
     CDS             complement(1851..2546)
                     /gene="minC"
                     /locus_tag="ACHUN9_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0696910.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3066..3434
                     /locus_tag="ACHUN9_003608"
     CDS             3066..3434
                     /locus_tag="ACHUN9_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0696911.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3542..3943)
                     /gene="pliG"
                     /locus_tag="ACHUN9_003609"
     CDS             complement(3542..3943)
                     /gene="pliG"
                     /locus_tag="ACHUN9_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0696912.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4185..4478
                     /locus_tag="ACHUN9_003610"
     CDS             4185..4478
                     /locus_tag="ACHUN9_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0696913.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4550..5209
                     /locus_tag="ACHUN9_003611"
     CDS             4550..5209
                     /locus_tag="ACHUN9_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0696914.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5301..5747
                     /locus_tag="ACHUN9_003612"
     CDS             5301..5747
                     /locus_tag="ACHUN9_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0696915.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5954..6865)
                     /gene="hlyE"
                     /locus_tag="ACHUN9_003613"
     CDS             complement(5954..6865)
                     /gene="hlyE"
                     /locus_tag="ACHUN9_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0696916.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1767 a         1535 c         1608 g         1973 t
ORIGIN      
        1 gtgagcagaa gatattcatc agcaacgatt acattagtca ttttattttg ccgacggcct
       61 cattgtcgaa agataagcgt acgacagtat tatctgaaaa gagtgatttt ttatccaact
      121 acacttcagc gcactgcgtt taaaaaatgc ctctttctta tgcgggatat catcatttca
      181 tcatgatgtc tttggtgagc ggtgaacaca atacacctgc gctgtctctt caggatgaat
      241 cccctggtct ggtgcctgcg ggctgatgtt gcagcagagc ttaggtcact tagacgctac
      301 tatcatttat ccaatggcat ggaatcgaaa tcagtcgata cccgcagtat atatcgtgaa
      361 ctggtcgttc tgaccatcaa cgattaaact gcgcttcggc gcagttttcg tttacagggg
      421 gctaaaaggg aaaattctgg ggcaaaaaaa gcccgccagt tacggcggga aacctcatcc
      481 tatgggagaa caatgaataa tgaaattgcg gggttatcat cccccagtgt atccacacta
      541 acaataaggt tatttactca gccaggcata aacattatgt tctgtgcgtg ggaacagcct
      601 taaggtgtaa agggggaggt ggaaatagca atgaggagta tcagcaagaa tactcgccgc
      661 tttaccacaa cgtggatgag agggatgaaa aactcaaggc agaaatgact ctgccttgaa
      721 gataaatgcg tttttacagc gggcttattt cagctcttct gcttccggta aggtcacgtt
      781 cagctcaaga atagaaatat cgccatcttt ttgctcaagc tgtacggtta ccatctcagg
      841 atcaatttgt acgtatttac aaatgacctc aagaatatct ttacgcaact gcggcagata
      901 atgcggttct gcatcgctgc gacggcgttc agcaacaata atctgcagcc gttcttttgc
      961 aatgttggct gtgtttttct tccgcgagag aaagaaatcg agtaatgcca taacttatcc
     1021 tccgaacaag cgtttgagga agcctttctt ctcttcttca atgaagcgga aaggacgttc
     1081 ttctcccaac agacgttcta cggtatctgc gtaggcttta cccgcatcgg cgttaatgtc
     1141 gagaatgacc ggttcacctt ggttagaggc gcgcaatact gattgatcct ctgggatcac
     1201 gccgacgagt ttgatgcgca ggatctccag cacatcttcc atgctcagca tgtcacctct
     1261 gcttacgcgg cctgggttat agcgcgttaa cagcaggtgc tctttaatag gctcttcgcc
     1321 attttctgcg cggcgtgatt tcgacgccag aatgcctaaa atacggtcag agtcgcgtac
     1381 tgaggagact tccgggttgg tggtaataat ggcttcgtct gcaaaataga gtgccattaa
     1441 cgcaccggtt tcaatccctg ccggggagtc acaaacgata aattcaaaat ccatcgcttt
     1501 cagatcatca agaactttgg cgaccccttc acgggtgagg gcatctttat cgcgtgtttg
     1561 cgatgccgga agaatataga gattttcagt acgcttatct ttaattaacg cctgatttag
     1621 cgttgcatcg ccctgaatga cgttgacgaa atcgtaaacg acccggcgtt cgcaacccat
     1681 aatcaggtcg agattacgca ggccgatatc aaaatctatc acgacagttt tctttccctt
     1741 ctgggccaaa ccagtggcga tggccgcgct ggaggttgtc ttaccaaccc cccctttgcc
     1801 cgaagtaaca acaataatgc gtgccataga aattccttgt taaaaaggga tcaatttaac
     1861 ggttgaacgg tcaaagcgtt ttcgactaac tgcagtcgcg ccgctttgcc ataaaattct
     1921 gctgggattt gatcactcag ccagtattca cctgcgatgg acaccaattc cgccatcagg
     1981 ttcgtacaaa atatttgcgt ttcccggtca ccacttgccc ctgccagcgc acgaccgcgc
     2041 atcatgccat agacatgaat gttcccatcg gcaatcaatt cggccccagc gctaacgtgg
     2101 cttgtaacaa tcagatcaca ttgtggagca taaatacgct gaccggaacg caccggggta
     2161 tctattaaac gcgtttttgt gaccggcgtg gtattttgcg ctggagcctg tggtgcggga
     2221 gctggacgtg gcgctttttc ctttccttcc gtcaggatag gcagccccat tttttcaatt
     2281 tcggctttaa gttgcgcgtc tttgcagcca cttacgccaa taacccgcaa accggttgcc
     2341 gaaaccgcct tatgcatcgc tgaccagttt accgggtctt ccagtgcact gacgttgagt
     2401 acaacggggg catgttttaa aaatgcgggg gcctgagcga ttttgtcttc cagcgcctga
     2461 tggataacct taggttctgc ctcatgcaga tgaaccacag ataaagtgaa gctactgcct
     2521 ttaagctcga ttggcgtgtt tgacatcctg gccttactca attagctatt aatcatcgcc
     2581 agcgcgcgat gatgttccga agactataag gcatgttata gtctggatta tattgaggca
     2641 agtcaccctc ccatttattc agagtaaaag tctattctgt gataaatggc gctgattcat
     2701 agcttaaaaa atacccttgt caatcaaccc attgccgccg tacttttgat tcttcttatt
     2761 tacgcttcct ttttccgcac cctaatcgtg acagcatctc cagacctgtt taaatatcca
     2821 gcattgaggc aagtgtattt tttcaccacg ccaggctttc ttaactttcc attttttgca
     2881 gcatcattaa agaacagata cccgttatct taatatccgc cacagaattg ctctatcata
     2941 gaaaccgcag aagacgtctg atacttctgt caggtcatcg taactcgtcc aggtcagcgg
     3001 ttttgattac ggattgatga cgaaatgagc ataagtgttg tacggttgga ttatcaggat
     3061 gtgctatgaa tatgatgcgt attatttata tcggattgtc aggtgtgggg atgatgttct
     3121 catcaatggc gtctggacat gacgctggcg gacttcaatc tccggcgtgc ggcgttgttt
     3181 gcgacccgta tatttgtgtg aactcagatg gcatttcccc agagttaaca aggaaatatc
     3241 tcggcgaaaa agccgctgaa aacttacaat cattacaagg ctacgatccc agcgaattta
     3301 cattcgctaa cggtgtattt tgcgatgtta aagaaaaatt atgtcgtgat gatcgttatt
     3361 ttggtgtaga tggtaagcgg agtggaaaaa tcaatcaaac caccacaaag atgttattta
     3421 tgtgtcgtga gtgatgcctt gtttacatta tgttctctgc tattcgtcat taacctcttg
     3481 agcgacagag aggagtgggg cgttaacgtc tatgatgggg atagtccccg tcggttgaca
     3541 tttacttaat ttgaatatcg acgttatatt ttttagtttt gtttttacgg gcgtcgtttc
     3601 gagtttgcag cactcgcagc tcatatttgc cagaagcagg gagtgagtat tgtccgtgac
     3661 tatcgagttc tggggaatat ctggataaat caacagaatc atcgattcct ggcccgaaga
     3721 gataagtatc agcgccttca ttagagatac tcacatgcac tttctgacct tttttggcat
     3781 agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc ggagtactga gcgctgctat
     3841 ggcctttgct gaactcaaca ttgacatttt tacccgcagc aaagctcgtg gaggttagca
     3901 gtgcaagcag caatacagcc ttactgatgc tcttgatttt cattggtagt ctcctgttca
     3961 gaatgagtac gatgaactgt tataatataa caatccctta cgggagaatt ccagtggctt
     4021 ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gggggattac ttataaaggg
     4081 gcatggactc aacaaaccgc agaaatctac agctggttgg cttgtggcgc gggtttcatt
     4141 gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt
     4201 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
     4261 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgt
     4321 tggatgggcg gaagaaactg gtcaatgctg atattgaaaa ggttaaactg gcattaaccg
     4381 agcaaggtta ctatttgcag ttaccgccac cacctgaaga tttgctgaag caacatcttt
     4441 ccgtcatggg gcagaaaaca gacgacacta acaaataacc gatatccggc ggtggcatta
     4501 tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
     4561 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag
     4621 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
     4681 tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt
     4741 cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggcgcgacgc tgcgtcaggc
     4801 aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg atctgacatt
     4861 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
     4921 tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg gcgatccaca
     4981 aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga
     5041 catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta ccctcaaggc
     5101 cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
     5161 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
     5221 gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
     5281 acgtaatggc ggaacacctg atgagcgatg tacctttctg gcaaagtaaa accctggacg
     5341 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
     5401 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
     5461 atattaaaac ttgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
     5521 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgatc tgcgcttatc
     5581 gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc gctacttact ggttcgaaag
     5641 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
     5701 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
     5761 tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
     5821 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg taactctcaa
     5881 tctttaaaaa acgtctgtgt ttttaaatgt gataaacctt ataccttgag tacatggaga
     5941 gaataatcgc tgatcagact tcaggtacct caaagagtgt ctttttaccg tgttttttct
     6001 gatactcatt acaggtgtta atcatttttt tggccgcttc ttttagcaaa gaaagcatta
     6061 aatcatcata atcaacgtag aatctggtgg tttcagtttc cgtttttatc tctccgatgg
     6121 cggctatttc ggtggttaat ttcaatttgg cggcatcgat atctttattc gcttgtttaa
     6181 ccgtgttaga cagggtggta aagaaactct gcacagattt taacttgttc ttcaattctg
     6241 ggatcagttt cccttcaact acgcccgcag caatagaata ggaaatgatt aaaccaaatg
     6301 gaccggcgac gacaccggct gcggcaccgg catacgcttc cttcctgatt ttatctacct
     6361 gtgactggaa atagctgctt ttttctgaaa aatcattggt taactggcta tctaacgcca
     6421 gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac cagcagggat ttttgcgctt
     6481 cattcagctt cgtgatgccg tcatccagta ccttaatgag aatgtctttc tgggcggatg
     6541 ctttcttctc attgtactca tcaaatagca aaatatacgc agcgagcaat tgcgtcgcaa
     6601 caccacacca ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa
     6661 gtaaggtttt aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc
     6721 gacttaactc ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt
     6781 tattataaag atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg
     6841 ttttatctgc aacgatttca gtcataatca ttcgcctctt taa
//
LOCUS       DAZOYS010000203         6826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOYS010000203 DAZOYS010000000
VERSION     DAZOYS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.204"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHUN9_003614"
     CDS             complement(155..667)
                     /locus_tag="ACHUN9_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0696917.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHUN9_003615"
     CDS             862..1626
                     /locus_tag="ACHUN9_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696918.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHUN9_003616"
     CDS             1577..2980
                     /locus_tag="ACHUN9_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0696919.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHUN9_003617"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHUN9_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHUN9_003618"
     CDS             4337..5080
                     /locus_tag="ACHUN9_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0696920.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHUN9_003619"
     CDS             5084..6304
                     /locus_tag="ACHUN9_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0696921.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHUN9_003620"
     CDS             6308..6814
                     /locus_tag="ACHUN9_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696922.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAZOYS010000204         6740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOYS010000204 DAZOYS010000000
VERSION     DAZOYS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.205"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(189..674)
                     /gene="sixA"
                     /locus_tag="ACHUN9_003621"
     CDS             complement(189..674)
                     /gene="sixA"
                     /locus_tag="ACHUN9_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0696923.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(877..3021)
                     /gene="fadJ"
                     /locus_tag="ACHUN9_003622"
     CDS             complement(877..3021)
                     /gene="fadJ"
                     /locus_tag="ACHUN9_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0696924.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3021..4331)
                     /gene="fadI"
                     /locus_tag="ACHUN9_003623"
     CDS             complement(3021..4331)
                     /gene="fadI"
                     /locus_tag="ACHUN9_003623"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0696925.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4511..4795)
                     /locus_tag="ACHUN9_003624"
     CDS             complement(4511..4795)
                     /locus_tag="ACHUN9_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0696926.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5167..6507
                     /gene="fadL"
                     /locus_tag="ACHUN9_003625"
     CDS             5167..6507
                     /gene="fadL"
                     /locus_tag="ACHUN9_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0696927.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1670 a         1811 c         1615 g         1644 t
ORIGIN      
        1 ttcatttcaa catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa
       61 tgaaagtaga aagtgtttta tttatagatg gggagagtct tcgatagtag tgagattgac
      121 agatatccgc tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt
      181 ggaactcatc agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat
      241 gtgccgtttc cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc
      301 ggcgtttcgc ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc
      361 accagtaccg aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca
      421 acatcgccgc agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa
      481 tcccctactt cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg
      541 atttccactt tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta
      601 gtggtcagag gacgaaccga atcactggcg gcatcaaggg ctgcgtcgcc gtgacgcatg
      661 ataaaaactt gcatattgca ccgcttttgt taaccagatt cgtccgtttt ctttcatctg
      721 aaaccagaga aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc
      781 tgttttttac ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc
      841 cgccattcag cgcggattca tatagctttg accttcttat tgaaggtcag ttgcagttgt
      901 tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg
      961 ggaaccatac tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga
     1021 atcgatatag cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcgcc
     1081 aatatccccg tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt
     1141 cagcatcaac atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg
     1201 tgcgccaatg agcgggtaaa tggcgggatc gacctgtttt ttgcttttac gccctttctg
     1261 accataaaga tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga
     1321 agaaacaaca tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat
     1381 aattttagtc ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa
     1441 accaaatttc actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg
     1501 gatagcttca ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg
     1561 cacgacaatg ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg
     1621 cgccgatgta cccgcatgag gaataatctc taccagcggc attttttcca caggactgaa
     1681 gaaatgcagg ccaataactt gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg
     1741 taaagatgac gtgttcgaag caaaaatggt atgagcggcg caattttgct ccacttccgc
     1801 caccatctgt tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg
     1861 ggcaaagccg cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct
     1921 ggctttgaga tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc
     1981 atgatttatg ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc
     2041 agtgacataa gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa
     2101 tggcgcaggc ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat
     2161 actacgcaaa gcttgcgatt gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc
     2221 gtcatagccg ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc
     2281 tgtcgccgga taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag
     2341 cagcgcacga cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga
     2401 tgggcgatcc ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac
     2461 gtcatccacc agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat
     2521 catctctaat gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc
     2581 gggtaacaat ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca
     2641 aacgcgaccg tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat
     2701 agccgcgata accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg
     2761 cgccagtgct tccgcttctt gcgccgtttt gcagttgcca atcatgttga tgtctgctcc
     2821 ggcgataaaa ttgtccggtt tcgcggagat aaacaccacg ccgcgcaact ctttgttttc
     2881 acggagttgc ttaataatgg cgcgcacctg cgaggcaaat tccgccttca gggtattcat
     2941 tttctcatcc ggtacatcga tggtgatgat agcaatgttg tccagacgaa cattaagagt
     3001 aaacgctgat gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg
     3061 gcagcacagg cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc
     3121 tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca
     3181 agcacgttaa atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca
     3241 cgagcaaaac gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct
     3301 tcgtgcatat cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc
     3361 ggtgttgacc aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg
     3421 tagctgcgca gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc
     3481 aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg
     3541 tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga
     3601 atattgttgt cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct
     3661 tctttgagtt ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat
     3721 gcatcttgct gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc
     3781 atccgcaagc cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcgcaaa
     3841 cgcaggcgag agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc
     3901 agcacgcgcg ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca
     3961 ccggcaatcc ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc
     4021 tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg
     4081 gtaccgagaa caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca
     4141 aagaccagct gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc
     4201 accatcttcc ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa
     4261 ggcgtacgta aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa
     4321 acctgaccca taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt
     4381 cttaactatt tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca
     4441 gtgaagacag gagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgaacgtac
     4501 ccgagaggaa ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag
     4561 tgaaatcgat atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc
     4621 acggttcgga ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt
     4681 ctgcgcggtt tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg
     4741 tgccaacatc catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg
     4801 atttcctctg tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg
     4861 gaaagtgctg ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta
     4921 aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca
     4981 gctggatcgc gtttcttaga tcatatttga aaaaagatgg aaacatactt gcagcattcc
     5041 agctggtccg acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta
     5101 cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt
     5161 atggtcatga gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt
     5221 atctccaccc aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg
     5281 ggccgggctt attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac
     5341 cccgcattga ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac
     5401 ccggatgtga atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc
     5461 gcgcctacgg catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt
     5521 tggggcgctt ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca
     5581 ggcggctctg tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg
     5641 tatcgcttaa ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg
     5701 aaaattgaac gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct
     5761 cctgccgggc aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt
     5821 aataccaaaa tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc
     5881 ctgtatgaac tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt
     5941 gacttcaaag gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca
     6001 attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa
     6061 atgtgggaag tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg
     6121 gcttacatca gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg
     6181 ctgttccaga aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat
     6241 tactacgatg ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg
     6301 gctcagaatc gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg
     6361 acttacgcat ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag
     6421 agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc
     6481 ggtactaact ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgcata
     6541 gcaggtgact ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga
     6601 gttttgcaaa cagcctgttg gcagcttgcc ctttcaaatc aataagcggt atccacagac
     6661 aaacctgaag ggaaaggcat attttcaggc gttctgctcg tccttctcaa agagtttact
     6721 tttctgcatt tccaggatac
//
LOCUS       DAZOYS010000205         6715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOYS010000205 DAZOYS010000000
VERSION     DAZOYS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.206"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..952
                     /locus_tag="ACHUN9_003626"
     CDS             <1..952
                     /locus_tag="ACHUN9_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0696928.1"
                     /translation="ERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIFLP
                     KLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAK
                     LATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIR
                     RDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGW
                     YEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(992..1663)
                     /locus_tag="ACHUN9_003627"
     CDS             complement(992..1663)
                     /locus_tag="ACHUN9_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0696929.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1770..2003)
                     /gene="yedF"
                     /locus_tag="ACHUN9_003628"
     CDS             complement(1770..2003)
                     /gene="yedF"
                     /locus_tag="ACHUN9_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0696930.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(2000..3205)
                     /gene="yedE"
                     /locus_tag="ACHUN9_003629"
     CDS             complement(2000..3205)
                     /gene="yedE"
                     /locus_tag="ACHUN9_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0696931.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            3392..3805
                     /gene="yedD"
                     /locus_tag="ACHUN9_003630"
     CDS             3392..3805
                     /gene="yedD"
                     /locus_tag="ACHUN9_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0696932.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3839..5326)
                     /gene="amyA"
                     /locus_tag="ACHUN9_003631"
     CDS             complement(3839..5326)
                     /gene="amyA"
                     /locus_tag="ACHUN9_003631"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0696933.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(5404..5769)
                     /gene="fliT"
                     /locus_tag="ACHUN9_003632"
     CDS             complement(5404..5769)
                     /gene="fliT"
                     /locus_tag="ACHUN9_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0696934.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5769..6179)
                     /gene="fliS"
                     /locus_tag="ACHUN9_003633"
     CDS             complement(5769..6179)
                     /gene="fliS"
                     /locus_tag="ACHUN9_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0696935.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(6194..>6715)
                     /gene="fliD"
                     /locus_tag="ACHUN9_003634"
     CDS             complement(6194..>6715)
                     /gene="fliD"
                     /locus_tag="ACHUN9_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0696936.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1672 a         1756 c         1677 g         1610 t
ORIGIN      
        1 tgagcgggct tacaaaaact tcttccagaa tcgggctgct tttccccgat tcaaaaagcg
       61 gggacagaat gatgtattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg
      121 tatttttctg ccgaaactgg gctggatgcg ctaccggaac agccgtcagg tcacgggtgt
      181 tgtgaaaaat gtcactgtca gccagtcctg cggtaagtgg tacatcagta ttcagacaga
      241 aagtgaagta tcaactccgg ttcacccttc agcatcaatg gtcgggctgg atgctggcgt
      301 ggctaaactc gccacgctgt cagatggcac agtctttgag cctgtaaaca gttttcagaa
      361 aaaccagaag aagctggcga gacttcagcg acagttaagc cgcaaggtca aattcagcaa
      421 caactggcag aagcagaaac gcaaaataca gcgactgcat tcctgtatcg caaatatccg
      481 cagggactac cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat
      541 tgaggatttg aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg
      601 gcgcaatgtc cgggcaaaat caggtttaaa ccgttcgata ctggatcagg gctggtatga
      661 aatgcgccgc cagcttgagt acaagcagct ctggcgtggc ggtcaggtgc ttgctgttcc
      721 gccagcgtac acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaaccgcct
      781 gtcacaaagt aaattcagat gccaggtatg tggatataca gtgaacgccg atgtaaatgg
      841 cgctcgtaac attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc
      901 aggccgcccg ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca
      961 gggatcctcg tccttcaggg cgtggaggat gtcaacaaac aggttgaatt aactccgcgc
     1021 cctggttttt gacattacct accgcgcgcg ataccgggtg ccaggtgaac tggtttgctg
     1081 gcacacagcc gttggtagca atttctgatg cttctttacc gccaatctct tgccgcatcc
     1141 attcctgtgc agcttctggc gacagaacca gcgggcggcg gtcatgaatg tctaccagac
     1201 cctggtctgc tgcagcagtg acaattaaaa atccttcggc ttcgtcacca cgctcaaatg
     1261 gtgtgctacc tatcgcggcc ataaaaatag gttgtccatc agcacgatag ataaaaaaag
     1321 gctgtttttt gtcgccttct tttttccact caaaccagcc atcggcaaaa cagattgccc
     1381 gaccatgttg ccagagcggt ttaaacatgc gactggttgc ggcagtttct acgcgggcgt
     1441 taatcagcgg cggtttatcc caccatccgg gagcatatcc ccagaaaacc ggatccagat
     1501 gaaggtgttc atcacgttca ctgagcagaa gaactttggt tcccggcgcg acgttgtatc
     1561 tgccaatggg ttcgggatcg tagggaatat cgcgttcaat atcttccgcg agaagcgcaa
     1621 ggtaatcttc acgcgtttgg gattgggcaa agcgtccaca catagaaacc tcctgagatc
     1681 atactgaaaa gaataggaga gggggcgggt aaaatctatg tgtcagaggc aacaatgttc
     1741 ccgctgtggg ccacagcggg aatgagggat tacttctgaa ttaaataacg aatggtcggc
     1801 ccgtcttgct gaatatccag caccgtatag ccgtgattac gcgcatccag tggaatatta
     1861 ttgatcgact gtggacagtc gctcaccact tccaggattt cccctttttt taactgcggc
     1921 atcgcctcaa gggttgcgac tgccggataa gggcagggtt cacccaccat atcaaggcgg
     1981 taatcaggaa cgatattttt catgcgatct ccttagcagt ctgcggtgcc gcacggcgga
     2041 agaagcgttt ttcccagccg ataatcaaca tcagcgcagt aaacaacagc aaatatgtca
     2101 ccagcaggcc acccatcggg ccaaaggttt tcagcaggtt gattttgtcc cagtcggtgg
     2161 ccagcgccgg agcgaaatca tcccagtaat acgccagaat cgtcgagccg atcacattgc
     2221 ccagaccgac ccaccagtag tgcacctggc cttctaccgc gcggtacatc cagccggttt
     2281 cgcagccgcc tgccagcacg atgccaaaac caaacagtaa accaccaatt accgcgtttg
     2341 gtcccgccca catgattttg ggttcaacgc ctaactgtac gtaactgaag atcccgatgg
     2401 cactcaccgc cataccgata atgattgctt tcgccatatg ggtacgtccg gtgatccaca
     2461 tatcgcggaa cgccgaagta aagcagattt gcgcacgttc aatcagtaaa ccaaagccga
     2521 cgccaaacag cattgccagc cccagtttgg gttggttcat cgccgtgagc agcgcccagc
     2581 ccagcatgcc gaaaaagacc agcatcccga gacgaaaacg acgccgcgcc tgatccggtt
     2641 tttgcgtcag cggtgaggcg gcagaaactt tctgcatttt cacgggaata cggaagatgg
     2701 gcagcagggt aaagcgcgcg ccaaaccatg aaccaatggc agtggcgatg gcaaagaacc
     2761 aggcatgcag cgagaactga ggaatgccgg taaagaacgc cgccaggtta cagcccattg
     2821 ccagacgcgc gccaaaaccg gcgataatgc cgccaataat ggcctgcata atgcggatac
     2881 ggctgcgcgg catgcgcagt ttgacattgt tggcccacag cgctgcggca aagcagccgc
     2941 caaacatgcc gaggatcatc atcccgtcga tgcgggttaa tggcgatcct tccagatgga
     3001 taattttaaa ataaccccac tcttcagcat ggacgccaaa cagctgcagg agctgaccgc
     3061 cccaacgggt aaattcaccc gtgacagccc aaaaggtgcc agtaatgcca aaatagtaag
     3121 tggagagaat acccgccgcg atgaccgcag ggatgggtgc ccagaattta atcaaccagg
     3181 cgtgtttgaa ttgctgccat gacatgaatg tagcctctga actcagaagg tttgaaacaa
     3241 gaatcgcgaa tcatacatca tgcaaattta ttttcggggt aataattcac aaaaataata
     3301 gttagatcaa atttctctct gaacctggaa aggctggcga catcagggct ttaatgccac
     3361 aatcctcttt tcttctcatg caggacgaat gatgaaaaaa ctcgcaattg caggtgcgct
     3421 catgttgctg gcgggctgtg ccgaagtcga aaactataac aatgtcgtga aaacccccgc
     3481 gccggactgg ctggcgggct actggcagac caaagggccg caacgtgcgc tggtcagtcc
     3541 ggaagcgata ggcagcctga ttgtcactaa agagggcgat accctggatt gccgtcagtg
     3601 gcagcgggtg attgccgtgc cgggcaagtt gacattgatg tctgatgatc tcactaacgt
     3661 taccgtaaaa cgtgagctgt acgaggttga gcgtgacggt aacaccattg agtacgatgg
     3721 tatgacgatg gagcgtgtgg atcgcccgac ggcagagtgt gccgccgcgc tggataaagc
     3781 gccgttaccg acgccactgc cgtaatgaaa tctccccggc ttgccgccgg ggatgaattt
     3841 aaatcacctc ttcgataacc cacacgctga cgctgccgcc gttgcaaaag aaggtcgctt
     3901 cgccgttttc gtcggtcact acgctctctt gccgattccc cagaaaatca cgccaggttt
     3961 tattgccgta attctctccc agatgaatgg ttttttcgcc atcatccccg ttcgacatga
     4021 ccaccacgca gccgggatat tcgtcagtgc cactgcggct aaaggcaatg cagttcggat
     4081 ggtcgaaaaa taacgtctgt acaccgtggg cgaaacgctg acgggcgaga attaactcat
     4141 caagctgttc gattattggc atatctatcg gataggtttg cccgtcacca ccgacatctt
     4201 cgtaatgcgc accgtagagg tccggataga acaccgaagg aacgccattt tcccgcaaca
     4261 aaattagagc atacgccagc ggtttaaacc acggttcgac cggcgcttcg agggcttgca
     4321 acggttgggt gtcgtgattg gctacgaggg ttacggcgtg gaaaggatcg gcttccacta
     4381 atgtacccgt gaaaatctgc gtcatgtcgt agtcgcgtcc catgcgcgat gcttcatgga
     4441 atttcatctg cagcggcgca tcgaacagca tggttttgcc ttccacctga tcaatatacg
     4501 tttgcagttt atcgacttca tgcgaccagt actctgccac aataaacagc ggctttggcg
     4561 caacttcctg tacgtgttcg atccactctt tataaaacca cgccggaata tgtttaaccg
     4621 catcaagacg aaaaccgtcg cattgtgttt gttccatcac ccagcgcgcc cagtatttaa
     4681 tctcttccgt cacggcatga ttgcggaaat cgatattctc acccatcaga taatcgaaat
     4741 taccgagttc atcatcaacc tgatcgttcc agccttcgcc ggtgtagtcg ttaacaattt
     4801 taaatatgcc atcttcatca ggattttcga tatggtcgat accgctaaaa catttgaagt
     4861 cccagataaa ctgcgagtat tgcccggcac gggcggggaa ggtgtaacgc gtccagcctt
     4921 cgcactcaat gatttcttca tcaatttgcg tacggtcatc agcatttaca cgctgcacgc
     4981 gaatagcttc tttttcatcc gcccccattt tgtggttgac taccacgtcc agtagcaccg
     5041 caatgtcatt acgtttcaga gcgtcaatgg cggccagcag ttgtgcttta tcgccatatt
     5101 tagtagggat gctgcctttc tgatcaaact cacctaaatc aaataaatca taggagtcgt
     5161 agccgaccga atacccgccc gatgcgcctt tataggcggg cggcaaccag accatattga
     5221 taccaatatc attaaaaccg tcggcgcgct cggccagttc aggccagagc ttaccgcctt
     5281 ccgggtaata ccagtgaaaa cattgtaaca gcgtgggatt acgcattctt cgactccatt
     5341 caaggggaac attagaagcg tagccgtaat cggattattc gcgagccatc ggctcgttaa
     5401 gattcaaaag aggttatcct gcggagccag cacaaagccg ccctgatctc cataggcact
     5461 taacactgat ttttgcaccg atgactgacc gaccagtttc gccagttcat ccatacgaat
     5521 ctgtaataac tgctttacct tgctttcgtt gtcgagaatc aggcgcagca tcgggcggag
     5581 ctgctcctgc atcgtggtgg acggggcaac ctcttcagtc agatgtgcaa tctcctgcac
     5641 cgcattcaca tacgccattt cgctggcgat gagttcgtcc cattgttctt ccgttgccag
     5701 gcgtaacatg agctggcttt tatcgacgag ttgttgccag gcgaaataca aatgcggtgc
     5761 atggttcatc agactgggtc ctgaatcaaa gaaggggaga gtaaagactc tttccaggca
     5821 tcggcaatat tgcgcattaa tgcttccact tcttcgactg cggagacatc gttgcgtaaa
     5881 ttggcttgca gcaagcgcct gaccatatag ctataaagag caatcaagtt ttgggttagt
     5941 tcgtctttgc tctcttcatc aagactcacc cgcagcccgt tctcaatgat gttgatcgct
     6001 tttgacaaag agacgccttt gccttgctga ttgttgtcct gcataaacag gcgcgctcta
     6061 accagtgcgc tcagcactcc atcaaatagc atggtgacca gctgttgctg gctggcgctc
     6121 attacggcgc tttcgacgcc aatttgtgca taggcttttg agccgctcgc ggtatacatg
     6181 ctgacctccg tgatcaggaa ttactcattg cagtaaattg ttggctgaga taggtactgg
     6241 tgttattcag tttgctcatc atggtatcta attgcgtaaa ctgtgcagtg taacgcgcca
     6301 cggtgtcatc aatgctggca ctgacggata gatactgttt ggtcagtttt ttcagtgtgg
     6361 cgttaatact gtcctgtgcg ctatcaataa tgccatcatc ggcaaggtaa ccttttacct
     6421 cggtggcaat tttggtggta attcctgttt ctttaccatc gcctaccagc aattcacgta
     6481 cggatgctgt attttcattc aggacttttt tgagtttatc atcatcaatt ttcagcttac
     6541 ccgtcgtgcc gtcctgctta atcccgatct cgtttaatgt tttgaaggca ctgtcgctgg
     6601 caccgtttgc aaattgtgcg cggatgccgc tttgaattgt acgtacaacg ctgtctccga
     6661 gaagtgcacc attattttta tcttgtgctt cagcacctgg gtctacttct ttata
//
LOCUS       DAZOYS010000206         6641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOYS010000206 DAZOYS010000000
VERSION     DAZOYS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.207"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..561
                     /gene="ykgH"
                     /locus_tag="ACHUN9_003635"
     CDS             <1..561
                     /gene="ykgH"
                     /locus_tag="ACHUN9_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR0696937.1"
                     /translation="LLFLYVNKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTE
                     DVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGR
                     QVLDEIQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGF
                     FLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(804..1499)
                     /locus_tag="ACHUN9_003636"
     CDS             complement(804..1499)
                     /locus_tag="ACHUN9_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0696938.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1492..2919)
                     /locus_tag="ACHUN9_003637"
     CDS             complement(1492..2919)
                     /locus_tag="ACHUN9_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0696939.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2930..3649)
                     /locus_tag="ACHUN9_003638"
     CDS             complement(2930..3649)
                     /locus_tag="ACHUN9_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0696940.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4177..5031)
                     /gene="rclR"
                     /locus_tag="ACHUN9_003639"
     CDS             complement(4177..5031)
                     /gene="rclR"
                     /locus_tag="ACHUN9_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0696941.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILCLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5257..6582
                     /gene="rclA"
                     /locus_tag="ACHUN9_003640"
     CDS             5257..6582
                     /gene="rclA"
                     /locus_tag="ACHUN9_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0696942.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1811 a         1636 c         1447 g         1747 t
ORIGIN      
        1 cttttatttc tgtatgtcaa taaagataat ataaaagtga gttacagcct aaaaataaac
       61 cagacaacac caggcatact tgttaactgt gatagcaata ataactttgc ctgtcagacc
      121 acaatgactg aagatgttat acagcgaatt actacatttt ttcacaccag cccagatgtc
      181 aagaacagag aaataaagct ggaatggtca ggagataaga gagatttacc aactgctgaa
      241 gcggaaatat ctcgcgtgca ggcgtctatt atcaaatggt atgcgtcaga ataccataat
      301 ggcaggcaag ttctcgatga gatacaaata ccttcagcaa ttaacagtga gctttataca
      361 aaaatgatat acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact
      421 ataagctcac cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt
      481 tttctaagca ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac
      541 caacaaaact ctgggcagta ataaacagca ttctgataga gagtgcctgt ataaagctga
      601 agtgacaatg accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt
      661 aaatatcctg gcattactaa tgccaggata tttacagaag agcagcataa ccatgacgga
      721 acggggttaa gtcaggttgt ccttaacgct attttcaacc tcgtatgcct tcttcaggtt
      781 tatgttcaga tctcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc
      841 ggaccatgaa ccccgacgac tttgataagc tcaatatccg ccgttgagct gggaccactg
      901 atgatgttaa tacatgacgg cattcgttca ccggcctgcg ctttctgatg caatttttct
      961 gcgagttgcg ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagattcc
     1021 gggagcaggc tcaatgaacg cccgcgctcg acggcggaaa aaagaaccac gcctcccgat
     1081 tcggttaaac catattcagc atacacgaca cccactttag cctgctctgc ctgcgagata
     1141 ttctcggcac ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttca
     1201 ctaatcccca attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc
     1261 tctttacaca gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc
     1321 gtcaacataa catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt
     1381 tgggtaagcc gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt
     1441 cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa attcgctccg attatccatt
     1501 ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc
     1561 aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc
     1621 gccattgata aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc
     1681 tggatgacta ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat
     1741 ccctttttca gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac
     1801 cggacacacg ctgtcacaag cagtgcataa agagcaggcg tagggtaaat ctttaaaatc
     1861 tttatagccg ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc
     1921 atatccatga ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg
     1981 aatacagcgc agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac
     2041 aataaccaga tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag
     2101 ccaggtgttg tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa
     2161 tacatctacc tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt
     2221 tttaggcagc gtggtacaca ttcgcgcatt accttcattg gtcactaggc ataccgaacc
     2281 tgtctctgcc accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc
     2341 gcggattttt tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc
     2401 cagacgttcg tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac
     2461 aacatgagat ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat
     2521 cacctgaatg ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga
     2581 tttagatttc accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt
     2641 agcgtcttct ttggtttttg caaaccagac gtgaccgccg ttttgcgtca ctttttctga
     2701 gagctggtac agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg
     2761 atcgcgccac tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat
     2821 acgctgctgc gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat
     2881 gcgtgtctta aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca
     2941 tcaacacttc agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac
     3001 tgatgttcag caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca
     3061 ggtgcacaac cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg
     3121 tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca
     3181 catttttcag cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag
     3241 atgggtgata cacagctctc ccttgcaaac tggcacctac atcgactacc cctaatttat
     3301 taacaataaa agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc
     3361 attcgggttc atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag
     3421 ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg
     3481 gaatcgcttc tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct
     3541 ccgggaaatt tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc
     3601 ttgatttcag ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc
     3661 cattacttca tgcccattta tgcccgatgg ttcccctcct tccgggggat caatcatcaa
     3721 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtgtgacaaa
     3781 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3841 cacattttat ggggaaatcg acgttcattt ttggcataag aaatccattt aaaaaggcta
     3901 catcccttat acctattcag agctacttat tcaggggtta ttgagatata cactgatggt
     3961 gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat agtcggggtt ttatttaatt
     4021 gaagcaattt agtttgcatt tttatttaaa aactgagcta gagtccatca gaaaaaaata
     4081 tataaagccc aaaccaccgt tggaaataga aatagttgaa aggctcacaa ctcccggaat
     4141 gcggattatc tttgccctga tttctggcgg ttttattcag ggtgcaagct gtctgaccct
     4201 ttcccgatat tctcccggag tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga
     4261 ttcactggca tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa
     4321 catctgggcc gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga
     4381 aacatcacgg aaaagctggg caaaacttgc ccgagacatg tgggcgatgc tggccagcga
     4441 ttcgacggtc caggcgtgtc ctggcatttc cagcatttgc tgtattaccg caccaagacg
     4501 tggatgcaga agcaaacaga gaatgttttt ctctgtatta acctgtgcaa tccactcacg
     4561 caccgcgagg gtaaagaatg tagcgcagat ctggctacac agtgcatcca ctcccggcat
     4621 cgccgatctg gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac
     4681 gctgtggtta accggtgcta aaaacagcgt ttccggcaaa gacgttaaaa aataacgcgc
     4741 cgaatgttgc aaccgaagag tgccgcagac aatacaggtc gattcattat cgacatgact
     4801 caggcgatga gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat
     4861 ctctcccgtc ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac
     4921 cgataattcc ccggcaccat gttgaagctg ccagtcactt cctaacacgc aattcttatc
     4981 gatcgttcct tgtggagcgt taagcatcaa caaacggcta agggcatcca tatcagactc
     5041 ctgagtggtt tttgattaca aacaccaaca atcttagtaa ataactaatc taataccatc
     5101 aaatggttaa tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa ataccagacg
     5161 ataagacaaa atatcaagcg ttgtgaagaa atggtatttg ctcttttgcg tctatagtca
     5221 tgatgtcaaa tgaacgtgtt tcgacaggaa atcatcatga ataaatatca ggcagttatt
     5281 attggttttg gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc aggttggcga
     5341 gtggctctca tcgaacaatc aaatgcaatg tatggcggga cctgtattaa tatcggctgc
     5401 atcccaacca aaacattggt tcatgacgca cagcagcaca cagattttgt ccgtgccata
     5461 cagcgtaaaa atgaagtggt taatttttta cgtaataaga attttcataa tcttgcggat
     5521 atgcccaata tcgacgtgat cgacggccag gcggagttta tcaataatca tagcctgcgt
     5581 gttcatcggc ctgagggaaa tctggaaatt catggcgaga aaatttttat taataccggt
     5641 gcacaaaccg tggttccgcc aattcctgga attaccacca cgcctggtgt atatgacagc
     5701 accggattac ttaatctaaa agaattgcct gggcatttag gtattttggg cggcggatat
     5761 attggcgttg agttcgcctc tatgttcgct aattttggca gcaaggtaac cattttagaa
     5821 gcagcttcgc tgtttttgcc tcgggaagat cgggatattg ctgataatat cgcgacgatt
     5881 ttacgcgatc agggcgtcga tattatcctc aatgcccatg tggagcgaat cagtcaccat
     5941 gaaaatcaag tgcaagtgca tagcgagcac gcccaactgg cggtggatgc actgttaata
     6001 gcttccggtc gtcaaccggc taccgcttcg ttacatccag aaaatgccgg tatcgcagta
     6061 aacgagcgcg gggcaattgt cgttgacaag caattacata ccaccgcaga caatatttgg
     6121 gcgatgggag atgttaccgg cgggctgcaa tttacttata tttcactgga tgattaccgc
     6181 attgtgcgtg atgagttgct gggagaaggc agacgtagta ctgatgatcg gaaaaatgtg
     6241 ccttattccg tattcatgac accgccactg tccagggttg gtatgacaga agaacaggcc
     6301 agagagagtg gtgctgatat tcaggtggtg acattgcctg tagctgcaat tccgcgtgcc
     6361 agagtgatga atgatactcg tggggtatta aaagcgattg ttgataataa aacccaacgt
     6421 atattagggg catcactgct gtgtgttgac tcccacgaga tgatcaatat agtgaaaatg
     6481 gtgatggatg ccgggctgcc ttatagcata ttacgcgatc agatatttac tcatccgtcg
     6541 atgagcgaat cactcaatga tctattttca ttagtcaaat aaactcaaaa tcagacgcca
     6601 gaacaaatat tctggcgtct cagagaaaag aatcttatta a
//
LOCUS       DAZOYS010000207         6555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOYS010000207 DAZOYS010000000
VERSION     DAZOYS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.208"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            144..1319
                     /gene="mdtL"
                     /locus_tag="ACHUN9_003641"
     CDS             144..1319
                     /gene="mdtL"
                     /locus_tag="ACHUN9_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0696943.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1294..2253
                     /gene="yidZ"
                     /locus_tag="ACHUN9_003642"
     CDS             1294..2253
                     /gene="yidZ"
                     /locus_tag="ACHUN9_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0696944.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2410..3159
                     /locus_tag="ACHUN9_003643"
     CDS             2410..3159
                     /locus_tag="ACHUN9_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0696945.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3181..3747
                     /gene="yieF"
                     /locus_tag="ACHUN9_003644"
                     /gene_synonym="chrR"
     CDS             3181..3747
                     /gene="yieF"
                     /locus_tag="ACHUN9_003644"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR0696946.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3801..5138)
                     /gene="adeP"
                     /locus_tag="ACHUN9_003645"
     CDS             complement(3801..5138)
                     /gene="adeP"
                     /locus_tag="ACHUN9_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0696947.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5304..5969
                     /gene="yieH"
                     /locus_tag="ACHUN9_003646"
     CDS             5304..5969
                     /gene="yieH"
                     /locus_tag="ACHUN9_003646"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0696948.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6036..6509
                     /locus_tag="ACHUN9_003647"
     CDS             6036..6509
                     /locus_tag="ACHUN9_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0696949.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1611 a         1654 c         1658 g         1632 t
ORIGIN      
        1 aaatttggct aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat
       61 cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc
      121 ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt gccctggttt
      181 tactttatcc cgccgggatt gatatgtatc tcgttggttt accgcgcatc gccgccgatc
      241 tcaatgccag cgaagcgcag ttgcatattg cgttctccgt atatctggcg gggatggcag
      301 ctgcgatgtt atttgccggt aaagtggctg atcgttcagg gagaaagccg gtcgccatac
      361 ccggcgcggc gctatttatt attgcctcgg tgttctgttc actggctgaa accagcacgt
      421 tatttcttgc aggccgattt ctacaggggt tgggcgcagg ctgttgttac gtagtggcgt
      481 tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcattactca
      541 acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg attatgctta
      601 aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg gtactgatgt
      661 tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac
      721 gagaaaatag cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc
      781 tcagcgtttc ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca
      841 tgggttttga gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcatga
      901 ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca
      961 cctcgcaggt gttattcctt gcggcgggga tcacccttgc cgtttcacct tcccatgcgg
     1021 tttctctgtt tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga
     1081 tgagtcaggc gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta
     1141 ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtattggcg
     1201 catggaatat gctgatcggg attctgattg cctgtagtat agtgagcctg ttgctgatta
     1261 tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat
     1321 ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg
     1381 aagcggatga acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg
     1441 tttgacgacc cgctctttgt gaactcaccg ctgggcctgt cgcccacacc gctaatggtc
     1501 agcatggagc aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg
     1561 caccaccaga caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg
     1621 atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa
     1681 ctacgtaact gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt
     1741 ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc
     1801 attgattatg aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg
     1861 gcactgcatg aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc
     1921 tgggaacaga gcgacacctg ggcgctggac aatgtgttac aggagctggg gcgcgaacgc
     1981 acgattgcta tgagtctgcc ggaattcgag cagtcactgt ttatggcagc gcaacccgac
     2041 aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg
     2101 ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct
     2161 tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa
     2221 accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt
     2281 tcacaataaa atgtaaaaaa gttgtaacaa gcttgtctga atcgaacttt tagccgcttt
     2341 agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc attaagcatg
     2401 gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct
     2461 gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc
     2521 cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc
     2581 gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg
     2641 ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt
     2701 gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg
     2761 cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct
     2821 aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat
     2881 gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt
     2941 gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt gtggtccgtg
     3001 gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg
     3061 aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt
     3121 gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt
     3181 atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat
     3241 ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta
     3301 ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca
     3361 gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg
     3421 ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc
     3481 ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg
     3541 attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg
     3601 gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga tcggcaaacc
     3661 ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt
     3721 gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat gcatggcggg
     3781 tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag gataaacagc
     3841 agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc aatcttcatc
     3901 acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc
     3961 atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgagca
     4021 agacttgagg tcatcaacac gccaacgtag atcaacgcgc cagctgcagc gtagcctggc
     4081 accattcccg ccagcggcga cagaaagata accagcagga acagcagacc aacaaccact
     4141 gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat ataagccgta
     4201 acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac atacagcgcc
     4261 tgcttcatgc gcgggaattt ccccttctca tccgccagac ctgctttatc ggtcacgcca
     4321 atcagcgtac cggaggagtc aaacaagttg accaacatga aagagaaaat cacccctgcc
     4381 agcccgaggt taaacgaccc ggctaaatct acatgaccca caactgtcat tacgctcggc
     4441 ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca tccagcccag cagcgtcgtc
     4501 accacgatag aaaccagcac cgctgcgtga atgttgcgcg aggccagaat ggcgatgatg
     4561 aagaaaccga ggatccccag cagcacgctg tgagaagtca gattaccgat gctcaccagc
     4621 gtttccgggt tagcgacaat cacacctgcg tttttcagcc ccatcatgcc aatgaacaga
     4681 ccgataccgc tggtaatacc cacacgcaga cttaccggaa tgttagcaat catccagtaa
     4741 cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc cccagaagat tgcgcccata
     4801 ccgatctgcc acggcagccc cattgcctgg acaaccacaa aagcgaaaaa ggcgttaagc
     4861 cccatcgcag gagccagcgc caccggaagg tttgcaaaca atcccatcat aatgctaccg
     4921 aaagcagcaa tcagacaggt tgttacgaag acggcactgg tgtccatgcc agcaacgccc
     4981 agaatttgtg ggttaacaaa tacgatgtag accatcgtca ggaaggtggt gaacccggca
     5041 atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt taaaaactcg ttccagcatc
     5101 ccctgaccag aagtctgggt ggtatgttga tgactcatta tctatttccg aacaaggagg
     5161 gaaaatccgt cgctatcgta taccaaaatg cgacaatagg cgcgtttgtg agagactttt
     5221 tttattggat ttacttatac ggcaacgatt gcgttgcgca aatcggctct acgaaaacgt
     5281 taaactgatt aaaaaggaaa ggcatgtccc ggatagaagc ggtatttttc gactgcgacg
     5341 gtacgctggt cgacagtgaa gtcatttgct cccgcgcata tgtaacgatg tttcaggaat
     5401 ttggtattac gctcgatcct gaagaggtat tcaaacgttt caaaggtgta aaactgtacg
     5461 aaattatcga tattgtttcc cttgaacatg gtgttacgtt agcgaaaaca gaatctgaac
     5521 acgtttaccg tgcagaagtc gctcggctgt tcgattcaga actggaagcc atcgaagggg
     5581 ctgaagcgct cctgtcagcg atcactgcgc caatgtgtgt ggtatctaac ggcccaaata
     5641 acaaaatgca gcattctatg ggcaagctga atatgttgca ctacttcccg gataaactgt
     5701 tcagcggcta cgatattcag cgctggaagc cagacccggc gttaatgttc catgcggcaa
     5761 aagcgatgaa tgtaaatgta gaaaactgca ttctggttga tgactcagtt gccggtgcgc
     5821 aatctggtat cgacgcaggt atggaagtgt tctacttctg cgccgacccg cacaataagc
     5881 cgatcgttca cccgaaagtc accaccttta cccatctttc gcagttaccg gaactgtgga
     5941 aagcgcgtgg ttgggatatt acggcatagt tcttcacact cccttcactt accccgctta
     6001 aattggcgct caaaggtaag taaagggagt ttgatatgtc tgtttcacgt cgggtaatac
     6061 atcacggact ttattttgcg gttttaggac cgttaattgg tgttctgttt cttgtcctct
     6121 acatattctt cgcaaaagaa ccgctggttc ttttggtaat aatacaggta cttccattgt
     6181 ttctcttatt gtcgattact acgggagcta ttcctgcatt gttaaccggc gtgatggtcg
     6241 cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg ttgtctggct ggtggcatag
     6301 gtggcgtcgt tatcaccgag atctattgtg cagttattgt acatattaag ggcatggctt
     6361 cctcggagtt gtttgaaaac attctttctg gtgacagtct cgttgtccgc atcattcctg
     6421 cattgctggc aggtgtggtg atgagcagaa tcattacccg tctgcccgga ttggatattt
     6481 catgtcctga aacagactct ttaagttaag cgggatactt tatctttggg ctactcaaaa
     6541 gcagacagga tgtta
//
LOCUS       DAZOYS010000208         6389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOYS010000208 DAZOYS010000000
VERSION     DAZOYS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.209"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            61..1659
                     /gene="rtcR"
                     /locus_tag="ACHUN9_003648"
                     /pseudo
     CDS             61..1659
                     /gene="rtcR"
                     /locus_tag="ACHUN9_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1641..2399)
                     /locus_tag="ACHUN9_003649"
     CDS             complement(1641..2399)
                     /locus_tag="ACHUN9_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0696950.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2416..3246)
                     /gene="glpG"
                     /locus_tag="ACHUN9_003650"
     CDS             complement(2416..3246)
                     /gene="glpG"
                     /locus_tag="ACHUN9_003650"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0696951.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3291..3617)
                     /gene="glpE"
                     /locus_tag="ACHUN9_003651"
     CDS             complement(3291..3617)
                     /gene="glpE"
                     /locus_tag="ACHUN9_003651"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0696952.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3807..5312
                     /gene="glpD"
                     /locus_tag="ACHUN9_003652"
     CDS             3807..5312
                     /gene="glpD"
                     /locus_tag="ACHUN9_003652"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0696953.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5364..5969)
                     /locus_tag="ACHUN9_003653"
     CDS             complement(5364..5969)
                     /locus_tag="ACHUN9_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696954.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5972..>6389)
                     /locus_tag="ACHUN9_003654"
     CDS             complement(5972..>6389)
                     /locus_tag="ACHUN9_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696955.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1604 a         1680 c         1644 g         1461 t
ORIGIN      
        1 gattttccac aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag
       61 atgcgtaaaa cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc
      121 agtcagcgct ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc
      181 atcgatcggc tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa
      241 cgcgatatcg ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat
      301 aacccgtggg atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag
      361 tttcagccag aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag
      421 atttgctggt ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca
      481 cctccgcgca aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat
      541 ttaagccgtt ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat
      601 tttcttaagt ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc
      661 gaaaaagtgg cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc
      721 aagtcatttc tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc
      781 gcgtttgtgg aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt
      841 ggtcatgtaa aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc
      901 gccaacggcg gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca
      961 atgctgctaa aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg
     1021 agcagtgatt ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa
     1081 ggcaaatttc gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt
     1141 ctacgccagc gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc
     1201 acactcactg gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt
     1261 gcgacctctc cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg
     1321 cggatggcca cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata
     1381 aaccgtctgc gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc
     1441 gcagaggcag aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc
     1501 tgccgccagg caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag
     1561 ggcaaagcca gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg
     1621 acgtgggaag ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc
     1681 tgcattacgc ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg
     1741 cccatattga ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg
     1801 cgcgagttct caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc
     1861 gagccgtcgc tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag
     1921 ataaaatcga gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg
     1981 gcgagaataa tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg
     2041 gtgacaatac gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg
     2101 ccgatatcga taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc
     2161 ctctcttttt cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg
     2221 gaaggcagcg ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg
     2281 aggtcgcggc gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg
     2341 acataaccct gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt
     2401 tataaatccc tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc
     2461 taaacccacg gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc
     2521 aaaccatccg gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat
     2581 gccactttgc ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac
     2641 gccagaaagc ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag
     2701 ggcgctaatg agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc
     2761 gccgagatac caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa
     2821 cgcgtgggtg aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag
     2881 ccataacatc acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc
     2941 gatcatcatc acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata
     3001 acggcgataa tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg
     3061 cggatctgcc gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga
     3121 ctcatccgcc agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg
     3181 cgtcgccatg taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat
     3241 caacatcgtt gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg
     3301 tacgccacct ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag
     3361 accacatcgt agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga
     3421 taacacatca ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg
     3481 tcgttggtta aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga
     3541 atatcgacca gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata
     3601 cattcgaact gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac
     3661 agtttacgtc gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt
     3721 tttcaatgtt acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag
     3781 ctttaacgaa agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg
     3841 gcatcaatgg tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc
     3901 tggaggcgca ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg
     3961 gcctgcgcta ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg
     4021 aagtgctgct gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc
     4081 gtccgcatct gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg
     4141 gtaaacgcac cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa
     4201 aaccggaaat taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg
     4261 tactcgccaa cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg
     4321 ccacctctgc tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg
     4381 gcaaaaaata tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac
     4441 agttcttcga cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca
     4501 gccatattgt ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag
     4561 ataaacgcat tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg
     4621 atgtggaata caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc
     4681 tgctgaaagt gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga
     4741 cctactccgg tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc
     4801 gtgattacac ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg
     4861 gcggtaagtt gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt
     4921 attatcaggg cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg
     4981 aaggcgaccg cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat
     5041 cgctggcgcg tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg
     5101 cgggggcgat aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga
     5161 aatacctggt ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa
     5221 aacaaggcat gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat
     5281 atacgcagca gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg
     5341 gctatttgga ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa
     5401 atcgccaaac ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt
     5461 ctgcaccaat ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca
     5521 aaatcagtcc aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc
     5581 agaaagaaat ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg
     5641 aaattcgaga gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg
     5701 ttttttaatt caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg
     5761 aaaaaaagtg catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca
     5821 accaggagcg gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg
     5881 gctaagacaa taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca
     5941 gggggaatat aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt
     6001 catacatcgc gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg
     6061 tcattggttc gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta
     6121 aaggatgatt atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt
     6181 cctggttatc tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg
     6241 tcgaccaaaa gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg
     6301 cacggaaaaa catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat
     6361 cagtcatata ctggcaggca aatcgctgt
//
LOCUS       DAZOYS010000209         6384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOYS010000209 DAZOYS010000000
VERSION     DAZOYS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.210"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUN9_003655"
     CDS             complement(<1..356)
                     /locus_tag="ACHUN9_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696956.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTDTITE
                     ATLYENGLYTRHIRLSR"
     gene            complement(415..4272)
                     /gene="sigA"
                     /locus_tag="ACHUN9_003656"
     CDS             complement(415..4272)
                     /gene="sigA"
                     /locus_tag="ACHUN9_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0696957.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4634..5638)
                     /locus_tag="ACHUN9_003657"
                     /pseudo
     CDS             complement(4634..5638)
                     /locus_tag="ACHUN9_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5623..6000
                     /locus_tag="ACHUN9_003658"
                     /pseudo
     CDS             5623..6000
                     /locus_tag="ACHUN9_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6039..>6384)
                     /locus_tag="ACHUN9_003659"
     CDS             complement(6039..>6384)
                     /locus_tag="ACHUN9_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0696958.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1584 a         1426 c         1438 g         1936 t
ORIGIN      
        1 cgactgagtc tgatgtggcg cgtatacagc ccgttttcgt acagggtggc ctctgttatg
       61 gtgtcagtga ccagcatgtg ggagacaatg ctgtattctt ttttaaagca ctcaccagaa
      121 acggtgattg taatgataat ggtcttctcg atcgagatgt aaatacccgt ttcgttatac
      181 tgtccgtatc ggaaccatga gaactcagtt gcattccagc catgctccag aatatgtgca
      241 tttttttgcc tgatagtttc tttttttatt cgctcatgtt taaggcataa caggcagata
      301 aataatgtca gaaaaagaaa gctaataatg agagcaagag cgatatagtc tgtcacaggg
      361 taatacatat tctctacggt agaagaaggg ccgcaaacgc ggcccgggct gttatcagaa
      421 agagtaacgg aagttggcgt tgatcgcgtt atccacgttg tatttaccaa atgccgattt
      481 ctcaaactca agaccgaagc gaagattatc gcgaatttcg gcgttgagac caacattcat
      541 gagaatacga ccgtcttttt cacctttgat acgtttctca ccggacgcat cacgcagtac
      601 ggtttcaccg ttggcaaaca ggtcaaactg gtagccaagg ccggcgcggg ctgtgacttt
      661 ccagtcctta ccggagaagg atttacccac atcaacaccg gtacgcccaa tcagcggatt
      721 aaagtcctta tccttcatgg tcacgctcat tccgcgatcc tgccagtcga accgtttacc
      781 tgatacggcc ccataaacca gttctgcctg cggctcaatc caggagtctt ctgttacatg
      841 atagcggtag cctgcttcag cacccacata caaggaatga gaactgtagt cttttgttcc
      901 gagtccagca aaggtcgccg agtactcatt atcatggtga acatacttac taatcaggtc
      961 gatataggca ccagagtcaa atatagcgga agcatacaga cctgccccga cggacttcgt
     1021 cttgccactg aatgcattac tgctggcatg actgtcggta tacgtcatag tcagaccagt
     1081 gaaaaggtca agtccatcca gctcatgttt tttatccaca ccaatctgca catgggtgta
     1141 gttgtcactg tatccactag aagctgaacc tgctccgctc atgatgcggg cccatgcacc
     1201 ggcctcgcca ttggtgtcac gcagatcccc cattcgttta ttcaggttgt tgacttctgt
     1261 caggaaggct ttgtaattac cggacatata cgattttgct gcaccggaag atgctgtatt
     1321 ttcagccacc ttatatccgg tcagggtcca cgtggtatgt tcctgatcct ctttcgtgct
     1381 gatcacagga gatatgttgc tgaaacctat ggcctgagta ctggctttaa atatctcttt
     1441 attgcttcct tttggggctg ttatcaggtc aacgtttaat cctgagttat tatttcccac
     1501 tttctgtata tcgaccgaca gactgttatt gcttccggtt aactgatttt taacaatgag
     1561 tttatccgat tgatttacag actggctcag atcagcccgc ataatcacag cagtattagt
     1621 ggcatccagt gtgtttactg tcacagtatg gaaacccgaa ttctctatgt tactaaacaa
     1681 caaaagactg tcgcgggttt ttaatgattg cagggctgac tgtccgttta ctctccataa
     1741 ggtgttatac atgctggctg aggcagcagg agcatcaata cctccggtat aagaagtatc
     1801 aaagccaccc agcaaacctg tagccagcgg tgtgtgtgtg gttttgtcca ggttatcttc
     1861 tttatactgg tcaacaccaa acagaataga tgcttttttg tctgatttta tattaccatt
     1921 gacccaggca taattttgag cctttaacgt ggcaccatct tctgtcatat tatatccatc
     1981 tgacatatag attgccgggc tgtatttatt attttctgta ggggtgcctg ttaatgtcag
     2041 ggttccggcc agttcaatag ctttatctac atacattttt ccctgggcgg taacagtagc
     2101 tcctttgttg ataatcaggt ctgtattaat taaggataat gcaccagtca tagtaacagt
     2161 tgatttatca ccaacagtta ttttgctgtt ttgagcactc agactgccgt tgaaactact
     2221 ttcgcctgct gaatccccgg agcgaagctg ttgtctgaat ccaaaacctt cgccagtaat
     2281 gtttcttcct gagaacatgt caatgtatgc agttttattt cccagaacaa tttgagagtt
     2341 tttagcctct atgtgtcctt ttacattagc atcccggcct atactcaggg ttgcgttttc
     2401 taaatttaat ttccggaaat taaatttacg actttcccag tcgggctggg aaaaagaggc
     2461 tatctgattg ttggacttat attccgtatt gtaacgttta tttacagtgc tttcctggtc
     2521 ctttatggca gcagaatagt ctttttgaca taaaaaagga attggacagt tgttattgcc
     2581 ttctttaaat atggcatgtt ctgtcgcatg tccttgtaat gttaactgtg cattccggac
     2641 agagatatcg tttttgatat cgactgagcc atcaaagatc agattggcat tattgttatg
     2701 ttgctgggta gtattgataa tatgatttat gcttatatta cctgatacat tcccatggta
     2761 catatatgca tcagtattag taagtgataa tgttgattgc ttcacgtttg agttcgttac
     2821 ggttgttccc gcatctgttg ctgcgatttt ctgaaatgac tggtcatagc cgtttagatc
     2881 cagtgttccg ccatttctgg taaaatagat ttcgccatta ccgctttcgc ttaaagcgtc
     2941 tttggcattt atttttaccg tacctttacc tccggccatg taaattttat tgaaggcttt
     3001 ttcagcacta aggatgacag tcccattacc tattttaagg ttatttccct gtgctatttt
     3061 tacatccaga gtaccactac ctattttatg cagattatct ccggcaacgc ccttaacatt
     3121 ccattcaacg gtggtattat tatcaatatc tatcccggca cctttataag acttatcttt
     3181 tccgtacact ttatatgtct ggttcttatc gaatataagc ccgccaatgc caagatcggt
     3241 atcctgcttg agcgttaatt cccctcctcc tgagacagac aggtccttat cttttataat
     3301 cgatgataac tcttgttttt taccatccag ctgcatagtg tttgtgaaat tcttttttaa
     3361 attgtttacc agaatattat taaataacga gtagtttgtc caggctatat tggtactact
     3421 gagaaaacca actgtactgg taacaccatg gaaaacccat ttttgttgtt ctttatcata
     3481 tagataagaa ccagaaccac tatcgccaaa aattggtgag tttttaaaag gggttctgtt
     3541 ggtataaatg taactcttgt ttttccagtc cagattaaaa agacttgcgc ttaacaagtc
     3601 gggattatag ttttgtccaa acagatattg tttgccgttt aatatcgttg aggttgttcc
     3661 ggcacctgct ctgaagccaa ttatttgttc cttaccgttg tagtttacgc catatctttt
     3721 catcgcatct tcgggagaaa gtgagaagtc ggcactctcc gtaacccctg ttgtttccac
     3781 gacgaattta ttcagacgat gaaccgaaaa gtcgccagat gatactctgt tacttgcttt
     3841 atatgctgtc tgatcccatg actgtgttgt tattgcatga tgctgtgtac cgttatgagt
     3901 cgcagtaaca ctgtacgcac ctccaattga tgttgttgca cctttgttgg aaacagcaga
     3961 aaaatcaggt acaggtagtt ctggaaaatg aaatatcttt ccatctttca tcattaattg
     4021 aacatttgtt gcacccggtc tgaactctcc tctgttttga gcaaggtcaa gataatcgcg
     4081 ggtccatata ttatttatat ccagcattgc tgcaaatgct gtttcgccat aacttccata
     4141 tattgaggat aaaataatac cgaggataac tttctttctt gatgtaccga cactaacttt
     4201 ccgggtcagt tcagaaacag caactaatcc acctgtaata tgactatatt tcagtgaata
     4261 aattttattc atacgtgaac tccgatattt ctgagttgtg gttatgggat agcatttaat
     4321 tacaacgggg aactatcagt catggtacgt aactgataag ggtataatga taatcactgt
     4381 cagttatatt tgtatgacag atgtaacaaa atgaaaaaaa aagataagca caagtgattg
     4441 attggtcaga tattgatcac ttggtgtttg tattttgaac tttgcgatca aaatttgatc
     4501 tttgttgttt ccggtcaatc ctttacaggt tttctgtatt atccggggga gttaagtctg
     4561 tcgatgtccg acagactttt gatgatggaa acaacatgca gcgataatgt gcatgctgtt
     4621 ttgcaggtgg aactcagaag gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg
     4681 tgccgatcag gcctgcaaca tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg
     4741 ccgtgaatgc actgccgtcg ttaccggcca gattctgacg gtaaccggcg cgcagctgtg
     4801 cccatttcca ggcattaaat tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg
     4861 tgaaaccgct ggccggggtc agatccacat caaatgcggt ggtgaacatc gcgttgtgcc
     4921 aggagacacc ggccgtcacc tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt
     4981 cttttgtctc aatactacgg gggatcaggt tctgtgcgac cagtcccagc gtccagttgt
     5041 catccaggtc cgtactggca ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc
     5101 ggtcgccttt gaaggcgctg ctgtcataat ttctgaccag cacgttgtag ttgaacaggt
     5161 cgacgcgctg gaatttgggg gggaagccca gagaccattt ctgcccggcc gtttccagtt
     5221 ccgtagccag agcaataccc acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg
     5281 tcaggcggct tttgtccacc tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga
     5341 ggtcagcatc agagacttta ccgtcgacac tgacggtgcc gtgtgattta accatcagtg
     5401 cggcagagag gcgatcaccg ggaagggccg ccacagcaga aacgcccagc tgtgcggcgg
     5461 catgtgtgtt acggaagtcc cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac
     5521 cgtggttact ttcgatggcg cggtcgaagg ccttccactc atcactgatc cggtcggcgt
     5581 tgtcggtgat attatccgga ttggagagct gcgcgccgac ggacggaagc aggatgctgg
     5641 gctcatgcgc gacgcaaaat ccacgatgta tatatcagta ctaaaagcgc gacggcggaa
     5701 gaagctctga aactaatcgg tgaactgtac gccatagagc acgatatacg cgggttaccg
     5761 gtgtctgaac gcctggcggt caggcaaatg cagagtagac cgctcctgac ttccctgtat
     5821 aagctgatgc aggagaaaga acacacgtta tcgaagaaat gtcgtctggg agatgcgttc
     5881 cggtatatca ggaagcactg ggctgcgttg tgcaacttct gtgatgatgg cctggctgag
     5941 gtagataata atgcagcaga aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt
     6001 tcgaggatca ctcattccgt gtctgactgc ttcagggttt aattttgctt ctgctgactg
     6061 aatgatgatt gggcagcaag tgcaatatcc cacataaatc cagttaactc acgagccacc
     6121 gccgtaatgg ctacttgtga ttttttccct tttgctgtca gagttcggta acgagaacac
     6181 aatcgttgtt gagctttcca tgcaatatct ttggaatatt gtgttacaga gtccggtcgg
     6241 taatatataa gccatgctcc aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa
     6301 agcagacgtc tcagttcgct gtttccaact tttgttattc ctctgggacg aatactgttt
     6361 ccactggaat attcacctgg cacc
//
LOCUS       DAZOYS010000210         6381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOYS010000210 DAZOYS010000000
VERSION     DAZOYS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.211"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUN9_003660"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUN9_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0696959.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="ACHUN9_003661"
     CDS             2218..2766
                     /locus_tag="ACHUN9_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0696960.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUN9_003662"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUN9_003662"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0696961.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUN9_003663"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUN9_003663"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0696962.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUN9_003664"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUN9_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0696963.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1607 a         1565 c         1615 g         1594 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga t
//
LOCUS       DAZOYS010000211         6360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOYS010000211 DAZOYS010000000
VERSION     DAZOYS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.212"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(425..3910)
                     /locus_tag="ACHUN9_003665"
                     /pseudo
     CDS             complement(425..3910)
                     /locus_tag="ACHUN9_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(3978..6086)
                     /locus_tag="ACHUN9_003666"
     CDS             complement(3978..6086)
                     /locus_tag="ACHUN9_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0696964.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1191 a         2012 c         1675 g         1482 t
ORIGIN      
        1 aaaacttcat tggagaatct ttgatattct caatcttttc accataaacg tctaatattg
       61 atggttcacc agggtcatca aactgctcac cataaggtaa aatacccaat aaatttctcc
      121 aacacctatc tctaccattt tcctgaagtg attctgataa ttgtaaattt gctctgttat
      181 atgcagctgt cgcagatttt atattccacg cccctaaagc catagaagct gcaccgactg
      241 gggcagcaat gatcgttcca tcggcagcaa cacttatacc gaattttgtc atgccacttg
      301 catacagcct tgctgaatta gcagtattta aactagctgt aaacattttt gtcgggttaa
      361 acggaaggtt atccagttta ttatttatat catctataaa atgactacaa ctctcacatg
      421 agaataaccc caacggatct atcctgctca ccggattcag cggatactga tacagattcc
      481 acccaccttt cagcccaatg gggtcctggg tgatgtaccg tctctgcaac ggatcatagt
      541 accggtgacg attgtagtac agccccgact cctcatcata ctgctgcccc ggcagacggt
      601 atggctgata cacatgatgc gggttctcct catttagctg gttgccccac tcatcatacg
      661 ccccgctcca caccgtatcg ccgtcttcgc tgatgagcgc cagcggctgg ccccgatggt
      721 cgcagtggta aagatgaacg tttcgcactg gtgtgtattc cggctccacc tgtctggcca
      781 gttgctccac cgtcagcccg cactgcgcaa gccacacccg gctttcactg ctcacgcggt
      841 ctgcccggat ttcttcctcc agtctgtcca gcagccgcac cagttcagcc ggaaatacca
      901 cgccgtgccc gtcctcgctc ccttcctgct gcagcttctc cgccaggctg cggcgctgcg
      961 ctttctcccg ctcgccgttc tctgtctcga cccggatgag cggcgtgaag cttcccggct
     1021 cgtataccgt ctggatacgt gtggtgtcgg tctgtaccgt cgtcagcctg tcgccgtccc
     1081 agccgtacca tgtctcctcc ggtttacgcg acagcgacat ccaccccgtc aggtcacgct
     1141 cccgccgcca tacccgtttc gccattcgcc gtcccagcgg gtcgtagagg tagcggctct
     1201 cgaccagtgg ctcgccatgc tgtatccgcg tgtggaacac caggcggtgc tggctgtcgt
     1261 agtggtgggt ccgctcgtcg tccgtccgta tcacacccgt cgggatgcgg tccgtcttct
     1321 ccgtcagtct gccgtattca tcatagtggt aaacatagtg cgcatcctct gcgatgcggt
     1381 tatccggcca cgccgtgagc gtgctgtccg ggtgtagctc cgggtccggc aggcggtttc
     1441 ccgccgggtc ggtggcatac gggatgcgga tgtccaggtc cggtgcgagg gtgcgcaccg
     1501 tctcacggtg cagcctgtcc cgcgtatact cgaccagcgg cgtcccgccc agcttcatgc
     1561 ccgccaggta accgctgccg taggtcagcc actccaccgg cggcaggctg tccggcgtga
     1621 cgcggtttgc cagcccctgt tcgttgtagg cgtgtttcgt ctcatgctgc cacagcagtt
     1681 cccccgtctc cgggttctcc accgtctgcc gttcgcccgt caggcggccc ttatcgtcgt
     1741 agccgtagtg gacagcgaca cggtggcctt cgctcaggtg gctgatttct ctcagccagc
     1801 cgtggccgtc atactgccac tgctctgccg ggtcgccgtt caccgtccgg tgcgtgatgc
     1861 ggtccgacgc atcgtagtgc cagagggtga caagcccctc atcctcactc tgcgtgagtt
     1921 ttccggtcag gtcataatga taacgttgcg tccgcccgtc aaagccgccc tgctgtacca
     1981 gccggtccag cgcatcgtag ctgaagtcgc tgtgactgcc gttctcgtta gtcagactga
     2041 tgacacgtcc ggcagcatcg tactccatac tgcgcgtcag cccgccctgc gtggtgctga
     2101 ccgtctttcc ccacgcatcg tactgtgtct cgctccggtt gccgtccggg gtgataacgg
     2161 cagtcaggtc gcctgcggcg ttgtactcat actgcgtttc acggccctgt gcgtctttca
     2221 ccgaggttaa ccggccacgg ttgtcatagt ggcggtaaag gctgatacct tcctcgcggt
     2281 gaaccgccgt catctggccg aagcggtcgt attcatagcg ggtctggtag cccgagcagt
     2341 cagtgaacgc cagcaactgc ccgtagcggc tccacgtcat ctgccgggtg ctgcccgtcg
     2401 catccgttgt cgtcgccggt aactcactgt gcgcgtcatc gtagcggtag cgtactgtct
     2461 ccccgctgcg cgatgtctcc gataccagcc tgcccggttc atcatattcc cggcggctct
     2521 ccagcccgtc cgggtacacc accgccgtca gctggttccc gtcgttatag taaaatttcg
     2581 tctcccgccc gtccggtgtg gtgatgtccg tgatatcgcc ggacaccaca ttcagaccgt
     2641 actctgtccg ccgtcccgcc gcgtccgtct gcgccgtgag ccttcctgcc gcgtcatacc
     2701 cgctgcgcgt gacgctgccg tccgccagtt cttttttcac cacccgtttc agcccggccc
     2761 cgccttctgt atgcagcacc tcacgccggt tcaggctgtc ggtgacggtg atgcggtcct
     2821 gctcataaag atagcggtag cgcatctccg gccttcccgc gtaacggtgc gccaccatcc
     2881 ggcccgggtg ctgcgcgtca tacgtgaaag cgcgcacctg cgtattgctg cggtcatata
     2941 ccgccagcag ttcaccggct tccgtatacg tgtaccgcac cagtggcgca gcgggcaggc
     3001 tctccgggta tgccgggtcg tgcatcaacc acaccgccga aaggcggata cccctgtcgg
     3061 ggccgtattc ggtaccaggc agtgtgtcgg ggaacgctga ggctgagaga gggcgggaac
     3121 tgtcagaaga agatagcgaa gaggtgcggg cctcttccgc acgctgcgcc tgcgtggtca
     3181 gcaccagacg gaactcccgc ccggcaccgt ccgtcacgtc ggtgatttcc ccggccaggt
     3241 caccggcggc ctcacgccgg tacgtcagcg tccgccatcc cggtaagcac ccggtacggc
     3301 ggcagcggcg ctggcagtac gtcctcagca cccggcaccc gctcagcccc ccccagtatc
     3361 caccacggcc cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc
     3421 ggcgcagcgc cccccacagc cgcgccagcg tatggccgtc cggctgtgct gccttaccac
     3481 cgcgcaccag ccacattgac tcactgcggc tgtacaccgc ctcccccggc agcagcggct
     3541 caaagtgaat gctccgcccg ccgttgtcgt tgagtatcag tccgtcatca cgtagctgta
     3601 agcggatatc agaaggcgct ttccagccgg ggccgaaaac gcccaccggt gcaggcgtct
     3661 tcgtccggta gctgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagcgcaa
     3721 ggtccgtctc gccgggcagc accttcgccc ccagcagcgg atttaccggg ttgcccgaag
     3781 tcatcccgcc cggacacacc gagcacgcca cgccggtggg cgcgccaatt cttacacctg
     3841 ccgaaccctg gacaatggga ccgccatact gagtcatatc tccctgacgc gccgctggtt
     3901 ttccgctcat gattcaaatt ccttcttctg tttattttta atgctggttt gttactgaat
     3961 accgaacagg tacgggatca gtactctctc atatccggcg tatcctgctt ttcatcaggg
     4021 gtcttcggtg gggatttcga ggctgcacag ttccagttaa tcaacagtga gtcaccatta
     4081 acctgctcct taccctgcaa atgaattttt gtgccgttga gaaaaatttg tccgtccttc
     4141 gtcagcacca gccttgcctt accacagcag agctccagat gctcaccggc ggagtaaatc
     4201 gcggtctgcc cggtatcatc ctttttcgtt ttaccaacgg tgaaggtaac gttatttccg
     4261 actttgacgt cataacccag cccgaccctc aacgatttat gcaaaccgat ctgcgaggac
     4321 tgcatcagtg ccaccgaggt gttcataatg ccacctaccg tcgtctgata cgccacgccc
     4381 accgtcagcg cacgaacaag tccaatcgtt tccacctgaa cagaacccac tttaattatc
     4441 tggttactgc ccaccgtctc tatctggtta acgcccaccg tctctatctg attgttggta
     4501 accgcaatca tctgattgtt gccaatggtt tcagcatggt tgtttatcac atccgtggtg
     4561 cggttattca gcacctcggt gttcatgttc ttctgcgcgt ggatgtagac ctgttctttc
     4621 cctgtcgcat cgtcaaactt cagttcatta aacccgctgc ccttataggt tttcgaacga
     4681 atggtcatct gcgtcttcgt ccccggcagg ctgccgggtg tgcggttttc ctggtggtag
     4741 gtacgcccca taataatcgg ctggtccggg tcgccgttga ggaagtccac aatcacctcc
     4801 tgacccacac gcggtatcgc caggttgcca aagccggtgc ctgcccacgc ctgtgccaca
     4861 cggatccagc atgaactgtc ctggtttgac gggttatagc ggtcccagtt aaatttcacc
     4921 cgcacgcggc catgttcatc acagaagatt tcctcgcctg ccggtcccgt cacgacggcg
     4981 ctctgcgggc catccaccag cggtttcatc aacggctgtg gccgccatgt tctgtctgcc
     5041 gggattaccg caaaatggtt ctccagcgtg gtgcctgaac ccctgcgtcc cggtaccgcc
     5101 tgcggctgtt cgccgtgaag atcgctcgcc accacctgcc attcccggtt caggttcgcc
     5161 tgcggatgcc ccgtcagcac aattcgccgt cccggccata tctccggcga acggcttgtt
     5221 ccgcgcgcca cttctgcgtt gtttcgccag ccgtccatct gccagcgggc aaagttctgc
     5281 ccgtgggcac ccttgaaacg tccggggtag tcatacactt catactgcgt gcgctggtaa
     5341 tcctggtact ggccttcctg atcaaaacgc cctggccagc cgggacgttt aaaggtgtag
     5401 tctttggtca ctacggaaga aggacggatt tgtgcgctgt agcgaaactg gctgatgcag
     5461 agggtactta cctcggtacg ggtgttcggg ttccagggga tctcaaagga ctccggcaga
     5521 taacgcacgg tgtcgcacag gaccaggctc tggtcggtac ttttttgcgc gtgctcctca
     5581 taaaagaaga tgccttcctc cgccgccatc cggcacagga aatcgtaatc agtttcgccg
     5641 tactggacac aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc
     5701 acaccgtttt ccttcaggat cgtcgcgagg atgctttcga tgtcctcatt ctggaagata
     5761 cggaagttct ggcgcagccc tatgcgccac agcggtgggc ataccttcat gctgtacagc
     5821 atctggtttt tgtcgttctc ccccagttca aaccaggtca ccacaccttt cacccggcgc
     5881 tgtacgtcat cgccctgcca taccgtcagg taggccattt tgtccagcac ctgcgcaaat
     5941 tcaagggaga gaaactgctg gctgaccaga gagagatcga gggaaaagag cgaagagagt
     6001 gactggttca aatgaaagga aaccaccgca aaagcatccg gtggcaggcc gtccacttcc
     6061 agtgtgaaac gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac
     6121 atattgcatg tacaacatgt cgggcggatg agataagcga gtcgacatct ggcgttaccg
     6181 gggaatatca gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag
     6241 aaacgggtgt ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt
     6301 tattcacgac aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa
//
LOCUS       DAZOYS010000212         6351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOYS010000212 DAZOYS010000000
VERSION     DAZOYS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.213"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(109..744)
                     /gene="msrQ"
                     /locus_tag="ACHUN9_003667"
     CDS             complement(109..744)
                     /gene="msrQ"
                     /locus_tag="ACHUN9_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0696965.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(745..1749)
                     /gene="msrP"
                     /locus_tag="ACHUN9_003668"
     CDS             complement(745..1749)
                     /gene="msrP"
                     /locus_tag="ACHUN9_003668"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0696966.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1858..2271)
                     /gene="hiuH"
                     /locus_tag="ACHUN9_003669"
     CDS             complement(1858..2271)
                     /gene="hiuH"
                     /locus_tag="ACHUN9_003669"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0696967.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2404..3075
                     /gene="hprR"
                     /locus_tag="ACHUN9_003670"
     CDS             2404..3075
                     /gene="hprR"
                     /locus_tag="ACHUN9_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0696968.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3075..4433
                     /gene="hprS"
                     /locus_tag="ACHUN9_003671"
     CDS             3075..4433
                     /gene="hprS"
                     /locus_tag="ACHUN9_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0696969.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(4541..5392)
                     /gene="hchA"
                     /locus_tag="ACHUN9_003672"
     CDS             complement(4541..5392)
                     /gene="hchA"
                     /locus_tag="ACHUN9_003672"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0696970.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            5539..5949
                     /locus_tag="ACHUN9_003673"
     CDS             5539..5949
                     /locus_tag="ACHUN9_003673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696971.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(5983..>6351)
                     /locus_tag="ACHUN9_003674"
     CDS             complement(5983..>6351)
                     /locus_tag="ACHUN9_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0696972.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT         1745 a         1332 c         1516 g         1758 t
ORIGIN      
        1 tgaggttgat gacaaacgaa acataacctg atgtgatctg cgcaggccgg acagggcgct
       61 cgcgccacat ccggcaggga taacgcgtac tttgtcggca atatgaagtt atacagataa
      121 tttgttgtga acctgcttgc gtaaccgctt aaaaagggaa cgcaacttct tataccgtaa
      181 ggctaaaagc agtacagcca gcccagcgta gatgagcggc tgcggtgaaa taatcttcac
      241 tgaccacaga taatgtattg gggccaggat cgcgacaaga tagacgaaat tatgcaactg
      301 ttgccagtgc ttgccgagtt ttcgctgcat cgcctgggtt gaagtaaacg ctaaagcaag
      361 caaaataacc cagctgataa ttcctaacgt taaataaggc cgggtaatta actccttgcc
      421 taataacgcc agattattca cgcccagctc cagcaatgcg taactggtta aatgcagtgt
      481 cgcccaggca aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt
      541 tgcgtagcgt gccagaggtg tgattaacaa ggtcgccagc aaaaatttca gtgcagtgcg
      601 accggtaaaa tgctgaatat ctttcaccgg atcggcaccc agtccaccgt gattgatcgc
      661 ccagaccagc cagagaaacg gcaacaatcc ggcaagatgc aggctaactt tcagccatgt
      721 cacctgtttt gctgtcagac gcattcagaa attctcccgc aaatccaggc cacgatacag
      781 cgatgccacc tggtcggcgt aaccattaaa cagtagcgtt ggctggcgct gaacatcgag
      841 gatgccgcct gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg
      901 cggattaacg ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg
      961 cggacgctcg cgggtcagct taatactgac tatcgattta atccctttaa agccatattt
     1021 ccacggcaca atcagtcgta ccggcgcgcc attttgtggc ggtaacgcct tgccataaac
     1081 acccacggtc atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata
     1141 aggatatttc agcccgccgc cgataaagcg gtcctgctgg cctggcattt gttccggtgc
     1201 ataaattgtt tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa
     1261 tttgtgcagc ggaaaaccaa tccacggcac caccatcgac catgcctcca cgcagcgcat
     1321 acgataaata cgctcttcca gcgggaagcg acgggttaaa tcatcgtgat cgagggtcaa
     1381 tggttttgcc acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc
     1441 attagcggcg ggatcggctt tatccagccc gaattcatag aagttgttat aaccggagac
     1501 tttatctact ggcgtcagtg gcagattatt ttgccaggcg gtaggcttgc tgaactccag
     1561 cggttttccg gcgggggcgg gcgggcgatc gttcccttta aaccagctaa gcagatcggc
     1621 atgcgcagcg tgaggcaaag aaagtgcagc tgcgctgatg cccagtgctt ttaacacctg
     1681 tcgacgcttc ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt
     1741 ctttttcata agccatcaca cttttttgtc gttagccagc aagagccgtt tactgttaat
     1801 ctgtcaggga gtgtcctccc taacagtagc agagatctgc ttttacatat ttatgcatta
     1861 actgccacga taggttgaat acccatattg gctaagtaat aaaggcacat gataatgctc
     1921 gttcacttta ttaatatgaa attcaacggg gatctcaggg aagaaacttt caagattttg
     1981 tttcttgaaa tagtccccgg ttttaaatac gacacggtaa tcgcccgtag ttgcagtttg
     2041 ctcgggccac agtgccttaa ttcgtccatc cttatctgtt ttggcggtat taagttgtaa
     2101 ccagccgttg tccgctttct tttcaagagt gactgtcacg tcggcagcgg gttttccggt
     2161 ttgctggttc aaaatgtgca cgctaagaat gttttgttgt gcggcataaa ccaaagaagg
     2221 taatgaaaat gctgccgtta ctacggagag tactaaataa cgctttaaca tgtttatatc
     2281 cttgtcatgt gaatgagttt tgaggatagc gaaaaagcgg ccagggtaac cttacagcag
     2341 cattacaatt ttgtaatgaa ctaggctgtt tctataacat atgatttatg gcatattatt
     2401 ttcatgaaga ttttacttat tgaagataat caaaggaccc aggagtgggt aacgcagggg
     2461 ctttccgaag cgggttatgt cattgatgcc gtttctgatg gcagagatgg gctttatctt
     2521 gcgctgaagg atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc
     2581 tggcagatct tacaaacgtt aagaacagca aagcaaaccc ctgttatttg ccttactgca
     2641 agggattctg tcgatgacag agtcagaggg ctggacagtg gagcaaatga ttatctggta
     2701 aaaccttttt cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac
     2761 gctttgaatt caacattaga aatcagtggc ttaagaatgg actctgtaag tcaaagtgtg
     2821 agcaggaaca atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg
     2881 gcctccagag ctggcgaaat tatacccaga acggttattg cgagtgaaat ttggggaatc
     2941 aactttgata gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt
     3001 gatgatcctt ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta ttcattcgta
     3061 gcggtaaaaa aataatgaaa agattatcta taaccgtccg tttaaccttg ctttttatat
     3121 tgctgctgtc tgttgctggc gctggaattg tctggactct ctataatggc ctggcaagtg
     3181 agttgaaatg gcgcgatgat acaacactca ttaaccggac agcacagatc aagcagttgt
     3241 taattgatgg ggtaaatcca gatacgttac ctgtatactt taaccggatg atggatgtta
     3301 gtcaggatat cttgatcatt catggtgatg gcatcaataa aattgttaac cggacaaatg
     3361 tcagtgatga catgttaaat aacatacctg ctagtgagac aatcagcgca gctggcattt
     3421 acagaagcat tattaatgat actgagattg atgctttacg aattaatatt gatgaagttt
     3481 cgccatcatt aacggttact gtggctaaat tggcttcagc cagacataac atgcttgaac
     3541 agtataaaat taatagcatt ataatttgca ttgtcgccat tgtactttgc tcagtattaa
     3601 gtccgctgtt aatcagaacg ggattacgag agatcaaaaa gttgagtggt gtaacggaag
     3661 cgctgaatta taacgatagc cgggagcctg ttgaggttag cgcattaccg agagaactaa
     3721 aacctcttgg gcaggcgttg aataaaatgc atcatgcttt agtcaaagat tttgagcgtc
     3781 taagtcaatt tgctgacgat ctcgctcatg aacttagaac gcccattaat gcattactgg
     3841 gtcagaatca ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg
     3901 ccggtaacat tgaagagctg gaaaatattt cgcggttaac agagaacata ctgtttcttg
     3961 cccgggcaga taaaaacaat gttttggtga aactggactc gctttctctc aataaggaag
     4021 tcgaaaattt gttggattat cttgaatacc tttcagacga gaaagagatt tgctttaagg
     4081 tcgagtgcaa tcagcaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc
     4141 ttattgttaa tgccattaga tattcaccag aaaaatcgcg tattcatata accagttttc
     4201 ttgataccaa cggctatctt aatattgatg tcgccagtcc tggaacgaaa attcatgagc
     4261 ctgaaaaact cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg
     4321 gacttggtct ttctttagtc aaagcgattg ccgaattaca tgggggaagt gctacgtatc
     4381 actatctcaa taagcataat gtgttccgga ttatgttacc gcaaagaaat taacataggg
     4441 cttcagtacg ccataaatca accatgaaaa aacgcagcca cgtcgtgagt attaacgtgg
     4501 ctgcgattga ttatgcgttt acattcaaac gtaacaggga ttaatccgca taagctgcca
     4561 gcatttcctg cgccgccagt ttacccaacg cattcgctgc gaaaggactg tcgccggtga
     4621 gaactttacg gtccttatgt actcgcccgg tgatgtcgtc attaataatg ttcatgccca
     4681 ttttcttcag ctcttcgccg aagtaccagg tgagatgacc cggcatatag cctatctctg
     4741 gcgtttgttt gtctgcggcg tctgggaatg cgcaaatgga ataaccattc agtgggttat
     4801 cgccgtggcg aagcgccaga aaagccgccg ggccgtggca aagggagata acaaaacggt
     4861 catttttgat tgcccactgc aaagcagcag ctacgtcctg gctttctggt aaaccaataa
     4921 gtgcgccatg accaccagga acaaagattg ctgcatattc gctatcagcg ttgaggctgg
     4981 cgacaacatc cgcgagtttc ttcggattgc ggaacaacga tttatgctgc tcaaagaatg
     5041 gcattacttt ttcatctttg tgcggcatag cccagtattc aaacttggtc atcagaccgg
     5101 aaatggtcgc cacttcgaat tcgaaaccag cagcatggag atgatacaac ggcagcaacg
     5161 tttcaatcgg atggttaccg gtcgagaaca gttttccgtt atcggttggc aaataacgtt
     5221 cgtccgcggc gatcaccaga attttatgct taccgcgata cggttttgga tagtccacgc
     5281 catcaagatc agagacggga ctggtatatt ggctaagcga atattctgaa gggaagaatg
     5341 cattatcttc agcaatatcg acctgcggat ttttacttgt ttgaacagtc atagtgtatt
     5401 ccttattgtt gcttagttag ggtagtcact atatttgcga ctgagctggt cgctattttt
     5461 aaccagtctg gaaaattgag tcaatatatc gaaagcggta gtcggcgggt gtaagtatgt
     5521 aatgatgatg ctgcactggt gttgaatatt attcacgcca aattttcctt attgagcaac
     5581 gtcgtcgcaa gtggcctcaa tgattttaga ccattatcaa aggtacgggg cggcggggag
     5641 agatttagtg ctgtgaaata ttgtggtact gatgaatatt tatatcgcgg attggtagag
     5701 aaaagattat ttcatgcact atttttgcaa atatttctat atgggaagcg tgtttgttac
     5761 ggattaattc ataaagaaat gcatttgggt gttggtttga agagtcgctg gttgatgtta
     5821 ttgtgccagg aggggcaggg taaggtgctg tatcgatatg cattggcaat tgcgtgtgcc
     5881 gcagatggag atgaaatgcc aataaaaaag cctgaattcg atgagggacg aactcaggct
     5941 agtgtttagt tttacagtaa cacgccgcag cacgttggct gattagaact ggtagactaa
     6001 gcccacacca acaacgtcat ctgttgcgat gccattgttg tcatagaaat cgtcatcttt
     6061 atccaacaag tttattttat agtcaacata tgttgagaag ttcttattga aatagtaggt
     6121 cgctccaatt tcaacatagt tgaccagatc ctgatcaaca tactcttgat gaccattgtt
     6181 gttatagtcg ttaccgccga ggtctttgcc ttttgaataa acatatgcaa gagatgggcg
     6241 cagaccaaaa tcgaactgat actgtgctac agcttcaaag ttctgggttt tatttgcgat
     6301 acccacatta ccatatggtg tcatattgcg ggtttcagca tacatcattg c
//
LOCUS       DAZOYS010000213         6327 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOYS010000213 DAZOYS010000000
VERSION     DAZOYS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.214"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUN9_003675"
     CDS             38..250
                     /locus_tag="ACHUN9_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696973.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUN9_003676"
     CDS             283..501
                     /locus_tag="ACHUN9_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0696974.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUN9_003677"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUN9_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUN9_003678"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUN9_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0696975.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUN9_003679"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUN9_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUN9_003680"
     CDS             1917..2660
                     /locus_tag="ACHUN9_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0696976.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUN9_003681"
     CDS             2690..3229
                     /locus_tag="ACHUN9_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0696977.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUN9_003682"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUN9_003682"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0696978.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUN9_003683"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUN9_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0696979.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUN9_003684"
     CDS             4582..6102
                     /locus_tag="ACHUN9_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0696980.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6327)
                     /locus_tag="ACHUN9_003685"
     CDS             complement(6135..>6327)
                     /locus_tag="ACHUN9_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0696981.1"
                     /translation="FGYSASELSDEALKARVTSSKSVSVKNGESTFSDAANEQASKVL
                     KPGNALRKYSVNPNKKGTY"
BASE COUNT         1668 a         1443 c         1506 g         1710 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag ctcgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaa
//
LOCUS       DAZOYS010000214         6276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOYS010000214 DAZOYS010000000
VERSION     DAZOYS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.215"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="ACHUN9_003686"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="ACHUN9_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0696982.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHUN9_003687"
     CDS             complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHUN9_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0696983.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAARRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="ACHUN9_003688"
     CDS             2736..3797
                     /locus_tag="ACHUN9_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0696984.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFYTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHUN9_003689"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHUN9_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0696985.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..>6276
                     /locus_tag="ACHUN9_003690"
     CDS             5678..>6276
                     /locus_tag="ACHUN9_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0696986.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVL"
BASE COUNT         1687 a         1591 c         1500 g         1498 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cctggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctgggatat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgccag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttctatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcatacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 gggggctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaa
//
LOCUS       DAZOYS010000215         6191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOYS010000215 DAZOYS010000000
VERSION     DAZOYS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.216"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            122..898
                     /gene="exuR"
                     /locus_tag="ACHUN9_003691"
     CDS             122..898
                     /gene="exuR"
                     /locus_tag="ACHUN9_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0696987.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1138..1221
                     /gene="yqiM"
                     /locus_tag="ACHUN9_003692"
     CDS             1138..1221
                     /gene="yqiM"
                     /locus_tag="ACHUN9_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0696988.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1243..1905
                     /gene="yqjA"
                     /locus_tag="ACHUN9_003693"
     CDS             1243..1905
                     /gene="yqjA"
                     /locus_tag="ACHUN9_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0696989.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1909..2292
                     /gene="mzrA"
                     /locus_tag="ACHUN9_003694"
     CDS             1909..2292
                     /gene="mzrA"
                     /locus_tag="ACHUN9_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0696990.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2439..2807
                     /gene="yqjC"
                     /locus_tag="ACHUN9_003695"
     CDS             2439..2807
                     /gene="yqjC"
                     /locus_tag="ACHUN9_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0696991.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2845..3150
                     /locus_tag="ACHUN9_003696"
     CDS             2845..3150
                     /locus_tag="ACHUN9_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0696992.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3153..3557
                     /locus_tag="ACHUN9_003697"
     CDS             3153..3557
                     /locus_tag="ACHUN9_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0696993.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3547..3846
                     /locus_tag="ACHUN9_003698"
     CDS             3547..3846
                     /locus_tag="ACHUN9_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0696994.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4032..4424
                     /locus_tag="ACHUN9_003699"
     CDS             4032..4424
                     /locus_tag="ACHUN9_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0696995.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4494..5479
                     /gene="yqjG"
                     /locus_tag="ACHUN9_003700"
                     /pseudo
     CDS             4494..5479
                     /gene="yqjG"
                     /locus_tag="ACHUN9_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5772..6137
                     /locus_tag="ACHUN9_003701"
     CDS             5772..6137
                     /locus_tag="ACHUN9_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0696996.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1561 a         1521 c         1546 g         1563 t
ORIGIN      
        1 aaatattcac aatattaatt caatacatta attcaatcac caaatttata tatgaccaat
       61 ggcataccca tgaataaagt ggtatgataa cttcgtccgc attccactcc gggagacgca
      121 tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc tgaaagagcg
      181 cattgaacag ggcgtctatc tggtgggtga taaactgcct gccgaacgct ttattgccga
      241 tgaaaagaac gtcagccgaa cggtagttcg tgaagccatc atcatgctgg aagttgaagg
      301 ctatgtggag gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc cgcgccatca
      361 gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt tgcttcaggc
      421 tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtaa cgaaacaaga
      481 catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct tccgtgattc
      541 cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact ccgccctggc
      601 ggccatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga aaaaactaca
      661 cgaacacatt gattcccgta ccgttgataa ctggtgtgat gaccacgatc aaattctcaa
      721 ggcgttaatt cgcaaagatc cccatgctgc taagctggca atgtggcagc atctggaaaa
      781 caccaaaatc atgttattta acgaaaccag cgacgatttc gagttcaatg ccgaccgcta
      841 tctattcgcc gaaaacccgg tagtgcatct cgatacggcc accagtggca gtaaatgaaa
      901 ctcctgtccg acaggcgctg cgacgcgcct gttcgcttcg tcggtaagcg aaaagtataa
      961 agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa atcagactcc
     1021 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
     1081 aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt tcaggctgtg
     1141 ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt agtgcatctt
     1201 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
     1261 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc gatgattggc
     1321 atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct tccagcggcc
     1381 tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa aggcgcgatg
     1441 ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg ctgggtcagc
     1501 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1561 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1621 ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc cgggttatca
     1681 gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct gtgggtattg
     1741 atcctgacaa ctctgggtta catgctcggc aaaacgccga tatttttaaa gtacgaggac
     1801 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1861 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1921 cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt cggcacgctg
     1981 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     2041 caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc tcatggcatt
     2101 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga ctccagcgac
     2161 cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta catcattgcg
     2221 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga taactctcat
     2281 cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat ttcaccgtaa
     2341 ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg acaatcgttc
     2401 ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc atcgctttag
     2461 ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt caggaaaagg
     2521 agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag aatcgtattg
     2581 acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc cagctgcgtg
     2641 ccgatcatca caagaaaatc gcaaagcaga aagatgaggt ggcggaacgc cagcaagatt
     2701 tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa cggaaactgg
     2761 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2821 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2881 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2941 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     3001 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
     3061 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
     3121 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3181 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3241 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
     3301 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3361 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3421 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3481 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     3541 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3601 cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc aacaggcgct
     3661 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtagc
     3721 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3781 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3841 ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt ttgaagattt
     3901 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3961 gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     4021 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc attttaatgc
     4081 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     4141 atatggaagc aatgggtgtc ccgggtttta tgctgccgct ggtgattctg cttgagttcg
     4201 gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg tttactgcgg
     4261 gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc gtcaactcgc
     4321 tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca attaccggtc
     4381 cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta
     4441 acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga aaaatgggtc
     4501 aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc ggcggtaaat
     4561 ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgca cctggcccca
     4621 ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatatt tcactcgcct
     4681 gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa ccgtttattt
     4741 ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc
     4801 cgggagcaac cggagatacg ctctatcaac atgaatttct gtatcagctt tatctccacg
     4861 ccgatccaca ctacagcgga cgagttactg ttcccgtact gtgggacaaa aagaaccaca
     4921 ccatcgtcag caacgaatca acagaaatca tccgcatgtt caataccgcg tttgatgcgc
     4981 tgggcgcgaa agcgggtgat tactacccac cagccctgca aacgaaaatt gacgaactta
     5041 acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt gccaccagcc
     5101 agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga
     5161 ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat attcgcctgt
     5221 ggactacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc
     5281 gcatcagcga ttacctgaat ctgtatggct tcctgcgcga tatctaccag atgccgggga
     5341 tcgccgaaac tgtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca
     5401 tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa ccgcaggacg
     5461 agatgttcgc ttcggttaaa aaaaggcacc ttacgaagtg tctctgataa ttagctaagt
     5521 gcatgttgtt catatcggat gcggcgtgaa cgccttatca aacctacaaa acatttatat
     5581 tcaatatgtt gcaatgaccg cgcaggccag ataagcgtag cgcatctggc gattttgcgc
     5641 ttgtcatcag tcttagcgcg ccttcacagc actttattaa ttccttgaac gaatatttac
     5701 tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa
     5761 ttgaggcaaa aatggactgg tatctgaaag taataaaaaa ttatgtcggt ttccggggtc
     5821 gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt acgttcgtgc
     5881 tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc atcctgacga
     5941 caatctacgg tattctggtg tttttaccgt ggtgggcagt tcagttccgc cgcctgcacg
     6001 acaccgaccg ctcggcgtgg tgggcactgc tattcttaat cccgtttatc ggctggctta
     6061 ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt ggacctgatc
     6121 caaaactgga accttaaaac aaatctgctc gccggatgtc tgactcatcc ggcttttatc
     6181 atcatgttga t
//
LOCUS       DAZOYS010000216         6131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOYS010000216 DAZOYS010000000
VERSION     DAZOYS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.217"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(230..1900)
                     /gene="betA"
                     /locus_tag="ACHUN9_003702"
     CDS             complement(230..1900)
                     /gene="betA"
                     /locus_tag="ACHUN9_003702"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR0696997.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1914..3386)
                     /gene="betB"
                     /locus_tag="ACHUN9_003703"
     CDS             complement(1914..3386)
                     /gene="betB"
                     /locus_tag="ACHUN9_003703"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR0696998.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3400..3987)
                     /gene="betI"
                     /locus_tag="ACHUN9_003704"
     CDS             complement(3400..3987)
                     /gene="betI"
                     /locus_tag="ACHUN9_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR0696999.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4126..6078
                     /gene="betT"
                     /locus_tag="ACHUN9_003705"
                     /pseudo
     CDS             4126..6078
                     /gene="betT"
                     /locus_tag="ACHUN9_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1244 a         1702 c         1706 g         1479 t
ORIGIN      
        1 ttgatttgtt cactcataat atatccagaa aataaaatct gccgcagata aggttgtatt
       61 aatagtctgt atcaggaatg ttcggggtaa atatcagcaa aaaaccagca tcatgaatac
      121 tggatatgaa gcatgagagt tacctcagtg tttatataag gattcggtcc ccctctctgg
      181 aacggtaact ctcaatctga tcggttcctg cgttagttca catcacgact cattttttcg
      241 ctctcaccgg catcccattt gccacaaaat atcccgccgt gctcctcggc agcgcttcct
      301 gtccacgaat catatccgct attttctcgc caatcataat tgtcgtggcg ttcaaattcc
      361 cggtgataat ctgcggcata attgacgcat ccaccacgcg caggccttcc agcccatgca
      421 cgcggccttc gccgtcaacc acggacatct cgtcgtaacc cattttgcag gtaccgcacg
      481 gatggaaggc ggtttcggcg tgattacgca cgaactcatc gagctgctca tccgtctgac
      541 attccgtgcc ggggctgatt tcgcggccac gatactgatc cagcgtcggt tgatgcatga
      601 tctcccgggt gatgcgaatt gcgtcgcgga actcctgcca gtcctgctcg tgtgacatgt
      661 agttaaacag aattgccgga tgctggtgcg ggtcgcggga tttaatccgc acatgcccac
      721 ggcttggcga gcgcattgag ccgacgtggc actggaaacc gtgctccttc accgcattcg
      781 agccgttata gttaatcgct actggcagga aatggtactg aatattcggc cacgcaaatt
      841 cctctcggct gcgaataaat ccgcctgctt caaagtggtt gctggcacca acgccagtgc
      901 cgccaaacag ccactccgca ccgattttcg gctggttcca ccactgcagg gcagggtaga
      961 gggaaaccgg ttctttgcac tcatattgca gatacatctc cagatgatcc tgaagatttt
     1021 cgccgacgcc gggtaattca tgcaccagcg gaatatcaaa ctccgccagc agttcagcgt
     1081 tgccgacgcc ggagcgttgc aggatctgcg gtgaggcaat cgcgcctgca cataacagca
     1141 cttctttgtt ggccgttgcg cgggttggga tggtgctgtc gccttccagc cattcgacgc
     1201 ccaccgcgcg tttgccgtca aaaatgatgt gatcggtcat agcgtgagta cgaatggtca
     1261 ggttagggcg cgatttggcc tgatcgagat aaccgcgcgc ggtactggcg cgacggccct
     1321 gcggcgtgac ggtgcgatcc atcggaccaa aaccttcctg ctgataaccg ttgagatcgt
     1381 ccgtgcgcgg gtagcccgcc tgcacgcccg cttcaatcat cgcttcaaac agcggattga
     1441 cgccgggttt ggaggtagtg acgctcaccg ggccatcgcc accgtggtag tcgttctcgc
     1501 ccacatcgcg agtctcggcc ttgcggtagt agggcaggca gtcgagataa ctccagttct
     1561 ccagaccggg ttcttgcgcc cagttatcga gatccagcgc attgccacgg atgtagcaca
     1621 tgccgttgat cagcgacgat ccacccaggc ctttaccgcg tccgcactcc atgcggcggt
     1681 tattcataaa cggttcaggc tctgtttcat aggcccagtt gtagcgtttg ccctgtagtg
     1741 ggaacgccag cgctgcgggc atctgggtgc ggaagtcaaa gcgatagtcc gggccgcccg
     1801 cttcaagcag cagcacggag gtattcggat cttcagtcag acgggtagcg agaacgttgc
     1861 cggctgagcc ggcaccaata atgatgtagt caaattgcaa ataaacctcc tggttagaat
     1921 atggactgga atttagccat ctcaacctgg atggacttca cctgggtgta actctggagc
     1981 gtcatcacgc cgttctcgcg accaatgccg gagtgtttgt agccgccaac gggcatctct
     2041 gccggggatt cgccccaggt gttgatccag caaatacccg cttccagctg atgaatgacg
     2101 cgatgcgcgc ggttcaggtc cgccgtcacg atgcccgccg ccaggccgta gtcggtatca
     2161 ttggcgcggc gaatcacttc gtcctcagat tcataagtga gaagcgacat caccggaccg
     2221 aagatctctt cgcgcacgat ggtcatttcg tcgcggcaat cggtgaacac ggtcggtgca
     2281 acccatgcgc cgttatcgaa gccatcgcct ttcagtacat cgccgccgca cagtacgcgc
     2341 gcgccttcct ctttgccttt ggcgatatag cgcagcacgt tatcgcgatg cgggaagctg
     2401 accagcgggc cgaagttagt ttgcggatcg aaaacgtcgc ccgcgcgaat gcgctcaacg
     2461 cgcgccagaa ttttctgctc aaatgcggct ttgcatttcg ccggaacgaa gacgcgggtg
     2521 ccattggtac acacctgacc ggagctgaag aagtttgcca tcatggcgat atcggcggcg
     2581 agatcgagat ccgcatcatc gaaaacgatc agcggtgatt taccgcccag ttccatggtc
     2641 acttctttca gggaagaggc cgccgagtta gccatcactt ttttgccgct ggcgacaccg
     2701 ccggtaaatg acactttggc aatgcccgga tgctcggtca gatattgccc ggtctccgcg
     2761 cccacccccg gcaacacgtt aaatacgccg tccggcaggc ccgcttcgct gtaaatttca
     2821 gccagcttta acgcggtaag cggggtgact tcgctcggtt tgaaaatcat tgcgttgcct
     2881 gccgccagcg ccggggcgga tttccacagg gcaatctgaa tcgggtagtt ccatgcgcca
     2941 atccccgcca ccacacccag cggttcgcgg cgggtataaa caaatgacgt ctcacgcaac
     3001 gggatctggc tgccttccag cgccgggatc agcccggcgt agtactccag cacgtccgca
     3061 ccggtaacga tatcgacggt tgaggtttcc gaatatgctt ttccggtatc gagggtttcc
     3121 agttttgcga gttcgtcatt gcgttcacgc agaatatcaa cggcccgacg cagaatacgc
     3181 gagcgctcca tagcggtcat cgccgcccag attttttgcc cctgttgggc actcttcacg
     3241 gcgcgatcga catcctcacg cccggcggcc tgcacggtcg ccagcacgtt accgttggcc
     3301 gggttaatgg tctcgaaggt gcgaccgctg gtggcggagg tataaccacc atgtatataa
     3361 agctgctgtt ctgccattcg ggacatcacg tctcctcggt taatcggtgg gtaaatgctg
     3421 agtaataaag tgacgggtca gcgagtgtgc gagaggttta tccagcggtt tgccgcttag
     3481 tgccgcgcgc agccataatc cgtcaatcag cgcggcaagg ccgtagcccg cttcctgtgc
     3541 ctgttcgcgc ggcaattcgc ggcgaaactc gctcaccaga ttcgacagca agcggcggct
     3601 gctgacctgc tgtaaacgat agagcatcgg ctgatgcata ctgctggccc agaacgccag
     3661 ccaggctttc atcgccgcac tgctcacctg agtttcatcg aagtttccgg caacaatcgc
     3721 ctgtaatcgc tgctctgcac tgccctgcgg aagtgcatgt aatcgattca aaaccgcgtc
     3781 acgcagctga ctggtgatat cgcgcatggt tgcttccagc agaccatttt tgtccctgaa
     3841 atagtggctg atgatccccg tagaaacgcc tgcacggcgg gcgatctgcg cgatcgttgc
     3901 atcgtgcatg cccacttcat ttattgcttc cagtgtggcg tcgatcagtt gtctgcgccg
     3961 gatcgactgc atccccaatt tgggcatttt cgccactcca ttcatcagcg ttgtttatct
     4021 attaaagcgg ttattgattg gacgttcaat ataaaatgtg tcgtaattgt tacgaatttg
     4081 attttaaatg gtaacaataa cagtggggat actggatgac agaccttttc cctgacaacg
     4141 atcctgtttc gcgacttctc ggccctgtgg attggccgca cgctggactg ggtttctaaa
     4201 accttcggtt ggtactatct actggcggca acgctctata ttgtctttgt ggtctgtatc
     4261 gcttgttcgc gttttggttc ggtgaagctc gggccagaac aatccaaacc ggaattcagc
     4321 ctgctgagtt gggcggcgat gctgtttgct ggcgggatcg gtatcgacct gatgttcttc
     4381 tccgtagccg aaccggtaac gcagtatatg cagccgccgg aaggcgcggg acagacgatt
     4441 gaggccgcgc gtcaggcgat ggtctggacg ctgtttcact acggcttaac cggctggtcg
     4501 atgtatgcgc tgatgggcat ggcgctcgga tactttagct atcgttataa tttgccgctc
     4561 accatccgct cggcgctgta cccgatcttc ggtaaacgga ttaacgggcc gataggtcac
     4621 tcagtggata ttgcagcggt gatcggcact atcttcggta ttgccactac gctcggtatc
     4681 ggtgtggtgc agcttaacta tggcttgagc gtactgtttg atattcccga ttcgatggcg
     4741 gcaaaagcgg cactgatcgc cttgtcggtg ataatcgcca cgatctctgt cacctccggt
     4801 gtcgataagg gcattcgtgt gttatcggaa cttaacgttg cgctggcgct gggattgatc
     4861 ctgttcgtat tgtttatggg cgacacctcg ttcctgctta atgcgctggt gctgaacgtt
     4921 ggcgactatg tgaatcgctt tatgggcatg acgctcaaca gttttgcctt cgaccgtccg
     4981 gttgagtgga tgaataactg gacgctcttc ttctgggcat ggtgggtggc atggtcgccg
     5041 tttgtcggct tgttcctggc gcgtatctcg cgtgggcgta ccattcgcca gttcgtgctg
     5101 ggcacgttga ttattccgtt taccttcacg ctgttatggc tctcggtatt cggcaatagc
     5161 gcgctgtatg aaatcatcca cggcggcgcg gcatttgccg aggaagcgat ggtccatccg
     5221 gagcgcggct tctacagcct gctggcgcag tatccggcgt ttacctttag cgcctccgtc
     5281 gccaccatta ctggcctgct gttttatgtg acctcggcgg actccggtgc gctggtgctg
     5341 gggaatttca cctcgcagct taaagatatc aacagcgacg cccccggctg gctgcgcgtc
     5401 ttctggtcgg tggcgattgg tctgctgacg ctcggcatgc tgatgaccaa cgggatatcc
     5461 gcgctgcaaa acaccacggt aatcatgggg ctgccgttca gctttgtgat tttcttcgtg
     5521 atggcggggt tgtataaatc tctgaaggta gaagattacc gccgtgaaag tgtcaaccgc
     5581 gataccgcgc cgcgaccgct ggggcttcag gatcgcctga gctggaaaaa acgtctatcg
     5641 cgcctgatga attatccggg cacgcgttac actaaacaga tgatggagac ggtctgttac
     5701 ccggcaatgg aagaagtggc gcaggagctg cggttgcgcg gcgcgtacgt ggagctaaaa
     5761 agcctgccgc cggaagaggg acaacagttg gggcatctgg atttgttggt gcatatgggc
     5821 gaagagcaaa actttgtcta tcagatttgg ccgcagcaat attcggtgcc gggctttacc
     5881 taccgcgcac gtatcggtaa atcgacctac taccgactgg aaaccttcct gttagaaggc
     5941 agccagggca acgacctgat ggactacagc aaagagcagg tgatcaccga tattcttgac
     6001 cagtacgagc ggcaccttaa ctttattcat ctccatcgtg aagcgccggg ccatagcgtg
     6061 atgttcccgg acgcgtgatt gtttttgcat aaaaccgcgc catgtctgca tatggcgcgg
     6121 gaatcattga c
//
LOCUS       DAZOYS010000217         6099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOYS010000217 DAZOYS010000000
VERSION     DAZOYS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.218"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUN9_003706"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUN9_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0697000.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUN9_003707"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUN9_003707"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0697001.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUN9_003708"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUN9_003708"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0697002.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUN9_003709"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUN9_003709"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0697003.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUN9_003710"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUN9_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0697004.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1450 a         1540 c         1646 g         1463 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatc
//
LOCUS       DAZOYS010000218         6097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOYS010000218 DAZOYS010000000
VERSION     DAZOYS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.219"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUN9_003711"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUN9_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0697005.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUN9_003712"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUN9_003712"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0697006.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUN9_003713"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUN9_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0697007.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUN9_003714"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUN9_003714"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0697008.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1619 a         1617 c         1449 g         1412 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgaga tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcacaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccg
//
LOCUS       DAZOYS010000219         5932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOYS010000219 DAZOYS010000000
VERSION     DAZOYS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.220"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            293..369
                     /locus_tag="ACHUN9_003715"
     tRNA            293..369
                     /locus_tag="ACHUN9_003715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:327..329,aa:Arg,seq:tct)
     gene            complement(414..1175)
                     /gene="envY"
                     /locus_tag="ACHUN9_003716"
     CDS             complement(414..1175)
                     /gene="envY"
                     /locus_tag="ACHUN9_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0697009.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1358..2248)
                     /locus_tag="ACHUN9_003717"
     CDS             complement(1358..2248)
                     /locus_tag="ACHUN9_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0697010.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2249..5221)
                     /gene="nfrA"
                     /locus_tag="ACHUN9_003718"
     CDS             complement(2249..5221)
                     /gene="nfrA"
                     /locus_tag="ACHUN9_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0697011.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5208..>5932)
                     /gene="nfrB"
                     /locus_tag="ACHUN9_003719"
     CDS             complement(5208..>5932)
                     /gene="nfrB"
                     /locus_tag="ACHUN9_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR0697012.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1560 c         1549 g         1476 t
ORIGIN      
        1 attctgatga taggatgagt atccataatg atcaccgacg ttggtttcat agttaccagt
       61 ctcataggag cggacaattt tccgttaggg cggtaaattg tactttgata catgaaaata
      121 cgggttttct tgattcaaac gcgcagcggt gtgcatttgt ttgccgctat agcgtaataa
      181 atcagaaaat cagacgcggt cgttcacttg ttcagcaacc agatcaaaag ccattgactc
      241 agcaagggtt gaccgtataa ttcacgcgat tacaccgcat tgcggtatca acgcgccctt
      301 agctcagttg gatagagcaa cgaccttcta agtcgtgggc cgcaggttcg aatcctgcag
      361 ggcgcgccat ttcttcttca tttaaccccg tcttatccgt tccgctttgc tcttcaccac
      421 atcacttttt gtcgctgttt ggcgagataa ttcaacggtg tcaggccgta aaacgcctta
      481 aaaacagaga taaagtacga cgtgctgcta tagccacata attgcgccac ctgagtgata
      541 tttttgttat ccatcaataa catctgtacg gcgtaacgca tacgacactc tgtgacaatc
      601 tggctatagc tgatattttc gttttttaat ttctttttga gcaggctggg gcttaaacat
      661 agcgaactgg cgacaattcg cagattccag taatgctgaa tatcgctttg aataatgcgg
      721 cagacgctgt cgcggacgct gctgcgtaag atatacatca gtagggcaat aaaccgcgat
      781 tgctcaagaa agttagacaa tacggtaaaa agcaatgcgc gcgtcaactc cgcttcgcag
      841 ggtttgctgg gttgctgctt gctgtggttg gcggctagcc ggaacacttc cggtgtacgg
      901 ctatgacaag cgataaccgg ggttgccagc cgcgaccagg gctttacaca ggttaagttc
      961 ttatttaaaa acaacagata atcgttgatg atgttacggt tgatgtgggt gattaatacg
     1021 tgatttaacg ttgagagttc aatgacgtta ttgttgcacg cgaaggcggc caggtagttt
     1081 ttcggaaggg taaacgtagc atggttattg acgcttacct ctacctcttt ttcggtcagg
     1141 atcaccacgc aaggttcact gctgctcaat tgcatttcgc actcctcaga tatcagaaac
     1201 tccgctcaaa ggatctatgc ttcctgcatg agtgatcggc ccgttcgccg ataacgatct
     1261 tctttcttta gcacgctttt tagcaattaa tcttgatgga attctgacga gagcgaaaga
     1321 ggtaagccag gtcgtacccg acttacctgg aggagattta atactcgaga atgccgtgcg
     1381 cgacgggcaa atagcgcaga gagaaataga gagtgtcttt accgcaagag gaacgtttcg
     1441 ctaacagcga ggcgatctct gcatccggtt tcggtttcgc tgtaaaggtt ttgcctttgc
     1501 cggcgacaaa cagttcataa acaatgccgc ttgctggggc ggagctttgg caatcggcgc
     1561 tggcctgtag ataacgttca cgctgttcag cggtaagttt atccacgccg ctgccatcct
     1621 gtacccagac attaactccg gttgctttaa cttgttccag cagttggcga tagccatctg
     1681 gcgacatgtt tccggcgaaa aaactactga tataaaccgg ttttgctgaa acatcgctaa
     1741 tcagccgctg cgcgttgttt aaccatgtta gcaaaggctg acgggcagct tcgctgcgcc
     1801 agttcaggtc gtcaatttcc gccctgatgt accagccatc cggcgttatg ccaggcgcgg
     1861 cgctccataa tctggcttgc tggagatcgg cagccagcag gcgattaaga tagctttcca
     1921 gcgctgcgga cgactgtttc tggtgcataa aaaattccgg atcggcgttc agcccgacaa
     1981 tcagtttcag gccagcctgt tgcgcagctg cggcacgctt aaacaataac gtgcgctgtt
     2041 ctggctgggt aaatgcatcg ccgtaacggg tccattgcaa aaccagggta tcgaagcctt
     2101 gcaaacgtaa ctgactcatc agcccctgcc actgggtatc ggtaacctga ctatctcggt
     2161 tttgtggttg ccagataata cctttcatcg caaaggaaaa agggctgacc aaaagcagtg
     2221 tcagcaatac gaaaatgaac ttacgcattt accagtgcac tccaatggtg agaaacgcgt
     2281 tgttgcgctc tccgttacgt tgattaatcg ccttaaaggt atgttgatac tcgacgccga
     2341 gactgacttt gtgcggccag gcgtcgtagt gcgtctcgcc ggtccagata ttccagcgga
     2401 ccccgactcc gccaagctgc gcgccctgag tgcctttatc acgatagccg ttgtcctgaa
     2461 cgtgagcgta aggctcaata gtctgtccgt tagctacctt ctgatgccag ctgacgcgat
     2521 aatctgccgt ccacgcctga atatcctggc ggatatattg cgccgcatcg aggtacaggt
     2581 tttgggcaaa ccagcctgaa ccgttcgggt gccattcgtc gctgtatttg ccgccattaa
     2641 agaatgaggc gctggcgcgc agcatggtat cggatgcgcc attttgcccg ttcagcggca
     2701 actgctgttc gacggcgagg aaaaagatct gatcgcgcag cggcttccag cgcagaccgg
     2761 tgccggacat cggatttttc accggcatca tcaccccgtt ttctccggta tcggcaaaga
     2821 cgcggctata aactgagagc aggtcgcctt ccagcagcat attgcgtccg atgcggtact
     2881 cggcttccag ttgcccgtag ctacgatagc ttttccctgg cgctgcgccg ccaacattat
     2941 tgttagcggt actcattgcc ccggaacgca agccgataga agaatcgaaa ctgaacgtcc
     3001 agcggcgacc gacctcctca tgcaaacggc ggaaattgaa gcgttgttga ttttgctctg
     3061 gggtcagtgg ggttatcagc gcctgattat caatgtcatc aatcaccagc cgggcgtagt
     3121 gctgcgtcgc aggcatgtca tccagacgct ggttcacgta ggccagttgt cggatcagtg
     3181 ccggatcgtc cggaagcctt ttatgcgcct gttcgagcat ttcccgcgac tgtgcgatat
     3241 caccgctatc ccacaaggcg taaccaagcg ctgcctggat gttgctatta ttcggttcca
     3301 gttccagcgc ggcgcgcaaa tcactcaccg cggccgggac attatgacgt tggcgataaa
     3361 ttgtcgcccg cgcaacgtaa gcgttggcag aaggcgcaat attgattgag cgcgtgagat
     3421 cgttcagtgc gagttccggc tgaccaggaa tgtaacgttg tgcatgcagc caccagtaga
     3481 gggcattgct tcccagtcca cgttgttctg cctgttgtag ccagcgatcg cgagccgcac
     3541 catttcctgc cgcctgggcg gtattggcag cagcaagcag atcctcattg ctcatgtcgt
     3601 gaagactgat tttctgccag gccgccagtg cggtggcgta gtcctcaacc tgatacgcct
     3661 gataggctac cgcacgatgt tgccaggcgc tcggttgtcg ttgttcggcc tgaagccatg
     3721 catacaacgc cacaccgggt agcgtgtccc gataacactt tgccagacgg ttccaggcgg
     3781 cagcatcgta ggaaggcgac atatcgccca gcaagcgaac tattgccggg caattatctg
     3841 caatacccgg caactgactt tgccactgac gttgctccgc cagcggtaag ggtttcgata
     3901 aaatcgccac cttcgccggc gttgccaggt aaggatgact ttccagcaga gacgccagtc
     3961 gcgccattaa agtctggctg acacgcgcat cgccctggaa aggatagcgt tgcagcaata
     4021 aatcggcagc ttcgcgtgac tgctcgttct gcatcagttg ccaggtcagt tgatccaggc
     4081 gggtaagatt tgccggttct tgctgataca gcaatcgtgc cagacgcaga gattcagcct
     4141 tgttacgggt cgccacgctg acagcataac gctcctcaag catttcattg gcggggaggg
     4201 tggcgagcag tttttgcgct gcgtcgtact gaccttcttt taacagcacc ggtagcgtcg
     4261 cgccaacaac atactggcgg ttatcggcaa actgcaccgt ataattcgcc aatgcctgaa
     4321 cggggttggc gctgtattta gataacagat agagccaact tttctcttgt gcgtccgtgg
     4381 taaacagcgg cttattttca ttgagataat gctggaggcg tgctttttcg ccacgataag
     4441 ccagcgcggt cgcgtaagta atatatgact gaggatcggt gaagatcccc tgtgattgca
     4501 gtgctaggat ccgatcgtcc agctgcccgg caagaagcac gtcaaaccac tgacggcgtt
     4561 ctgccgcgct taatgtgttc tgctggcgtg cttcattgta tagcgtatct gcctgggacc
     4621 attgtttcag gtagattgcc cgttgcagca gatcggttcg cagcgttttt ccttccggcg
     4681 atgcagcaaa cgtcgcatcg ttcagttgcg ctctggcgac aggtaactgt gcaagccgca
     4741 gggcattctg cccgacttca ctgcgacaac gcagggtcgg tgcagcatcg cacgcttttt
     4801 gctgggcaag cagttcttca acagttgtaa cgcttttcac ttcaaccgga atagccgcca
     4861 gactgcgctc aagtcgggca tctcctgggt gacgtttcag ttgatcctca agcaacagcc
     4921 gcgcccggtc atcatgacca aaatggcgat aggcttccgc aaggtataaa gtcagcggaa
     4981 tattatccgg cacctgctgg tgtatatatt caaattcgcg gatggcggtt gcttcgtcgt
     5041 tatttttctg tgccttcagc gccttatcga gacggggata aataacaaaa tggcgataat
     5101 cgctcagccc cagctcttct gcgctggtgc cgatattgtc tgcgagtgcg ctggtactca
     5161 ataaagacgt cagcagtaaa ccagaccatc cgatgacgcg attaaggtta ttctccttca
     5221 ttttcggact ccagttgcgc aacctgttct gtgtttaaac ctgctttgag taatagtgat
     5281 tgcatcgaaa cttgtaattc gcgttgaatt gtcaggacgc gatccaacgt ttcctggctg
     5341 ataacgcctt cggtgaccaa aaacttgccg agcggcagag aactgcgttc atggcgcaat
     5401 aacaacacgt taattgctga acgattaata tgaccgagcg tggtcagtat ttcggcgaac
     5461 aggaactgat gcggcacata ttgccgccag atttcaccgg cctgctgttc cgtgagccac
     5521 tgatgctgaa ccgcattgta caacattgcc cgcggatcgt gaccgcgtcg gcgtgcatac
     5581 cagtgacgta accccgtgac aatttgtccc cgcagaacaa tgaagtaacg cactttgcgt
     5641 ccgactttac gcgtcagggc cgccagcgaa accgggtcaa taccatcttc actgccgaca
     5701 attaactcat cattttccag acgcagcggc agtaccgcat aatgcagcgc cacggaggcc
     5761 ggcatttcgg caatcagcga ggaagggatc tgccaggcat cgatggattc ccacgccacg
     5821 ccgttttgct ctgccagcgc ctgtgccagc tgctcggcgc taatcagccc ctgcatcagc
     5881 attgaaccgc ccaagcgtag accttcgacg cgattacgca gtgctgtatc ga
//
LOCUS       DAZOYS010000220         5921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOYS010000220 DAZOYS010000000
VERSION     DAZOYS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.221"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            101..547
                     /locus_tag="ACHUN9_003720"
     CDS             101..547
                     /locus_tag="ACHUN9_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0697013.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            558..2009
                     /locus_tag="ACHUN9_003721"
     CDS             558..2009
                     /locus_tag="ACHUN9_003721"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR0697014.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLVLNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            1999..3069
                     /locus_tag="ACHUN9_003722"
     CDS             1999..3069
                     /locus_tag="ACHUN9_003722"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0697015.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3069..4817
                     /locus_tag="ACHUN9_003723"
     CDS             3069..4817
                     /locus_tag="ACHUN9_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0697016.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4867..5865)
                     /locus_tag="ACHUN9_003724"
     CDS             complement(4867..5865)
                     /locus_tag="ACHUN9_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0697017.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGKESTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQY
                     QLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1369 a         1497 c         1509 g         1546 t
ORIGIN      
        1 ctctgtctca aattgtcatt gtttaccgac atatcgcctc tcatcactta atcgctccac
       61 cattacaatt gattaaaata tattttatgg agtccgatta atggcagctc ttactgcaag
      121 ctgtattgac ctgaatattc agggcaatgg cgcttattcc gttctgaagc agttggcgac
      181 aatagcgtta caaaacggtt ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct
      241 gcgcgaaaaa atgcactcta ctggatttgg ttccggtgtc gccgtgccgc acggtaaaag
      301 cgcctgcgtt aaacaaccgt ttgtattatt cgcccgcaaa gcgcaggcta ttgactggaa
      361 agccagcgat ggcgaagacg tcaattgctg gatctgcctc ggcgtgccgc aaagcggcga
      421 agaggatcag gtcaaaatca tcggcacact gtgtcgcaaa attatccacc aggattttat
      481 tcatcaactg caacagggcg acgccgacca ggtgcttgcc ttgttaaatc aaaccctcag
      541 ctcataagga agtggcgatg gagtcatcct tatgtattgt cgcgatcacc aactgccccg
      601 ccgggatcgc tcacacctac atggtggcgg aagccctgga acagaaagcc cgttctctcg
      661 gccataccat aaaagtggaa actcaagggt ccagtggcgt tgaaaaccgc ttatccagcg
      721 aagagattgc cgctgccgat tacgtcattc tcgctaccgg gcgtggcctg agcggtgatg
      781 atcgcgcgcg gtttgccggg aagaaagttt atgagattgc catctcccag gcgttgaaaa
      841 atatcgacca gattttcagc gaattaccga caaactcgca gctttttgcc gcagatagcg
      901 gcgtgaagct tggcaaacag gaagtgcaaa gcggcagcgt gatgagccac ctgatggcgg
      961 gcgtctctgc ggcgctgccg tttgtcatcg gcggtggtat cctggtggcg cttgccaaca
     1021 tgctggtgca gttcggtttg ccgtataccg atatgtcgaa aggcgcgccg tcgtttacct
     1081 gggtagttga atccatcggt taccttggct tcacctttat gatccccatc atgggcgctt
     1141 atattgcctc gtcgattgcc gataaaccgg ctttcgctcc ggcgtttctg gtttgctatc
     1201 tggcgaacga caaagcgctg ctcggcaccc agtcgggtgc gggattcctc ggcgcggtgg
     1261 tgctggggct ggcgattggc tatttcgtct tctggttccg caaagtgcgc cttggcaaag
     1321 cattacaacc gctgctcggt tcaatgctga tcccgttcgt tactctgctg gttttcggcg
     1381 tgctcactta ctacgttatc gggccggtga tgtccgacct catgggcggg ctgctccact
     1441 tcctgaatac cattcctcca tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc
     1501 tggcgttcga tatgggcggc cccatcaata aaaccgcctg gttcttctgc ttctcactgc
     1561 tggaaaaaca catttatgac tggtacgcca tcgtcggcgt tgtcgcgttg atgccgcccg
     1621 ttgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg
     1681 ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct ataccttacg
     1741 ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggtggc attaccggcg
     1801 tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc
     1861 tgatcggcct gatgtcgccg gtgggttcgt tttatctggt gctagcgatt ggcctcgtgc
     1921 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
     1981 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
     2041 gtcagcggcg atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtt
     2101 aatgagatca cctttgatgg tctgggcagc tttgttgccc gtaaggggaa taaaggtcca
     2161 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
     2221 agcggttttc tgcgctttac caccattggc ggctggtgga atcagtcaat gctcaaccac
     2281 cgggtaacca tacgcacaca caagggattt aaaatccctg gtgtgattgg ttccgtcgca
     2341 cctcatgcgt taacggaaaa gcaaaagcaa cagccgctgt catttgatga gatgttcatt
     2401 gatattggcg cgaacagtcg cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt
     2461 attagcccgg aagccaattt tgcctgctgg ggcgaagata aagtggtcgg caaggcgctg
     2521 gataaccgca tcggctgcgc aatgatggct gaactattgc agacggtgaa taatcccgaa
     2581 attacgctgt atggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg ggcacaaacc
     2641 tcggcggaac acattaaacc ggatgtggtg attgtgctgg ataccgctgt cgcaggtgat
     2701 gttccgggca ttgataacat taaatacccg ctgaaactgg gccaggggcc ggggctgatg
     2761 ctgtttgaca agcgctactt ccccaaccag aaactggtgg cggcgttaaa aagctgtgcc
     2821 gcacataacg atttaccgct gcaattttcc accatgaaaa ccggtgcgac ggatggcggg
     2881 cgctacaacg tgatgggcgg cgggcgtccg gttgttgcgc tgtgcctgcc aacccgctat
     2941 ctgcacgcta acagcggcat gatttcaaaa gccgattacg atgctctgct cacgttgata
     3001 cgggattttc tgaccacctt aaccgcggag aaagtcaacg cgtttagcca gttccgtcag
     3061 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc
     3121 gcacgcctgg tgagctggcg caacagactg gcgtttcagg caggaccatc ctgcgtgata
     3181 ttgactatct caactttacc cttaacggca aagcccgcat tttcgccagt ggcagtgcgg
     3241 gctatcagct ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
     3301 acgacgatcg gctgctggcg ctgttattac tgaatacctt tactccccgt gcgcaactcg
     3361 cctcggcgct taatttgcca gaaacctggg tagcagagcg tctgccccgg ttaaaacagc
     3421 gttatgaacg cacttgttgc ctggccagtc gccccggttt gggccatttc attgatgaga
     3481 cagaagagaa acgcgttatc ttgctggcga acttgctgcg caaagatccg tttttaattc
     3541 cgctggcggg cgtaacacga gacaattttc agcatttatc cacggcctgc gacaaccaac
     3601 accgctggcc gctcatgcag ggtgattatc tctccagcct gattctggcg atttacgccc
     3661 tgcgtaatca actgaccgat gagtggccgc aatatcccgg taacgagata aaacaaatcg
     3721 ttgaacagag cggcatgttt cttggtgata acgctgtaag aaccctgaca ggtttgatag
     3781 agaaacagca tcagcaagcg caggtaattt cagccgatca tgtgctgggg ttgctgcaaa
     3841 gggtgccagg catcgcgtca ttgaatatta ttgatacgca gctggttgag aatattaccg
     3901 ggcatttatt acgttgcctt gccgcaccgg tgtggattgc cgagcaccgc caaagcagca
     3961 tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc
     4021 tactgcgtga acagctcgat attccccttt tcgatagcga tctgatcggt ttgtattttg
     4081 cctgtgcgct ggaacggcat caaaacgaac gccagccgat cattttgctc tcggaccaga
     4141 acgcgattgc cactattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga
     4201 ttattgcccg tagcttaagc gagcttattg ccattcgcga agagattgag ccgttattga
     4261 tcattaacaa cagccattat ttactggatg acgcggtaaa taattacatc accgtaaaaa
     4321 atatcattac ggctgccgat atcgaacaaa taaaacactt tctggcgacg gcatttattc
     4381 gccaacagcc ggaacgcttt ttctctgccc ccggaagttt tcattattcg aatgtacgcg
     4441 gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtggca caacaccata
     4501 ttaccgccga tgaagcgcaa cggatcatcg cccgcgaagg cgaaggtgaa aacctgattg
     4561 ttaatcgcct cgccatccca cattgctgga gcgaacagga gcgacgtttt cgtggatttt
     4621 ttattaccct cgcccaacca gttgaggtga ataacgaagt cattaaccat gtcttgatcg
     4681 cctgcgccgc cgccgatgcg cgtcatgagc taaaaatatt tagctatctg gcaagcgtat
     4741 tgtgtcagca tccggcagaa gttattgccg ggttaacagg atatgaggca tttatggagt
     4801 tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa
     4861 aaacgtttaa cgcaggctat tatagtcatc aaccatttcg ttgtactcgc gtaacgctcg
     4921 tggaaatgag tcttcgacca tccagcttgt gtttgcatcc tgaagatgct ctttatccca
     4981 gtcagagtac gggactttat ctctgacgcg gcgaacatat tttttagtct cgagttgata
     5041 ctggtctgcc gaattaatga acgagaaatt cataccgcct ttatccgctt ctttggcctg
     5101 cgctaccagc gtttccagtt cagagacttt cttcattgcc gcatcaacat caaacttctc
     5161 ttgcgaaatc aggttattaa tttgtttcgc tgaaatcatg actgccagag agtagtagtg
     5221 gaatgttttc ccttctcttt cttcaatatt tttcagttcc gcaagctggc gcttatcatt
     5281 aatttcctga atcgccgcat gataagattc tgctacaggt tcaaaggatt ccagattttt
     5341 taagaatgtc tggtgcagcg ttttcccttt cgcaaacgca tcgtctttat aattttcctg
     5401 ggtgtaatat ttatccattt cgttaatggt attacccaac gccacggcag catcaatata
     5461 actcaccgcc acgccatcaa taggttgcag cgcaggcgtt aacgccacag cactttttac
     5521 gcctttttca cactcagcga ggttggattc actaatgccg tagatgccat aaacagtact
     5581 ctctttaccg gtaggcccct gtttaaaatc tttcagccag tcagcataac gcgccagact
     5641 gttctgtaca gggatctgta acttgttgta gcacttgata tagacatcca ttttttcgtc
     5701 gaaaaccgct tgttcatcga ccaccggttg cgcaggtgtt tcaggtttag cttctgcttt
     5761 tgcaacggga gcttcaggag ctggtgcggc aggttgacta ttggcaggcg ggggcgtttc
     5821 tgtttcggct tttttatcat cacaacccgt cagaccgagg gccatgatgg cgactataat
     5881 agcggaagat aataaattcc ttttcattta attgccttat a
//
LOCUS       DAZOYS010000221         5872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOYS010000221 DAZOYS010000000
VERSION     DAZOYS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.222"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            208..831
                     /gene="nfeR"
                     /locus_tag="ACHUN9_003725"
                     /gene_synonym="yqjI"
     CDS             208..831
                     /gene="nfeR"
                     /locus_tag="ACHUN9_003725"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0697018.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(938..2458)
                     /gene="aer"
                     /locus_tag="ACHUN9_003726"
     CDS             complement(938..2458)
                     /gene="aer"
                     /locus_tag="ACHUN9_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0697019.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2876..4255
                     /gene="ygjG"
                     /locus_tag="ACHUN9_003727"
     CDS             2876..4255
                     /gene="ygjG"
                     /locus_tag="ACHUN9_003727"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0697020.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4297..4629)
                     /locus_tag="ACHUN9_003728"
     CDS             complement(4297..4629)
                     /locus_tag="ACHUN9_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0697021.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4848..5831
                     /gene="ebgR"
                     /locus_tag="ACHUN9_003729"
     CDS             4848..5831
                     /gene="ebgR"
                     /locus_tag="ACHUN9_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0697022.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1435 a         1585 c         1472 g         1380 t
ORIGIN      
        1 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
       61 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
      121 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
      181 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
      241 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
      301 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
      361 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
      421 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
      481 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
      541 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
      601 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
      661 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
      721 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
      781 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
      841 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
      901 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
      961 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     1021 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     1081 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     1141 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     1201 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     1261 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     1321 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     1381 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     1441 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     1501 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     1561 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     1621 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     1681 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     1741 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     1801 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     1861 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     1921 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     1981 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     2041 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     2101 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     2161 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     2221 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     2281 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     2341 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     2401 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     2461 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     2521 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     2581 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     2641 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     2701 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     2761 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     2821 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     2881 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     2941 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     3001 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     3061 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     3121 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     3181 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     3241 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     3301 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     3361 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     3421 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     3481 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     3541 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     3601 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     3661 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     3721 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     3781 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     3841 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     3901 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     3961 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     4021 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     4081 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     4141 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     4201 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     4261 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     4321 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     4381 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     4441 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     4501 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     4561 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     4621 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     4681 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     4741 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     4801 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     4861 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     4921 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     4981 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     5041 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     5101 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     5161 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     5221 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     5281 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     5341 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     5401 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     5461 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     5521 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     5581 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     5641 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     5701 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     5761 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     5821 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac ag
//
LOCUS       DAZOYS010000222         5865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOYS010000222 DAZOYS010000000
VERSION     DAZOYS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.223"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHUN9_003730"
     CDS             complement(<1..560)
                     /locus_tag="ACHUN9_003730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0697023.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUN9_003731"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUN9_003731"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0697024.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUN9_003732"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUN9_003732"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0697025.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHUN9_003733"
     CDS             complement(2371..3636)
                     /locus_tag="ACHUN9_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0697026.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHUN9_003734"
     CDS             complement(3863..5077)
                     /locus_tag="ACHUN9_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0697027.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHUN9_003735"
     CDS             complement(5177..5533)
                     /locus_tag="ACHUN9_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0697028.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHUN9_003736"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUN9_003736"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZOYS010000223         5863 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOYS010000223 DAZOYS010000000
VERSION     DAZOYS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.224"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..353
                     /gene="traA"
                     /locus_tag="ACHUN9_003737"
     CDS             <1..353
                     /gene="traA"
                     /locus_tag="ACHUN9_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR0697029.1"
                     /translation="LSVQGTSAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMMLFPEL
                     AMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVGFAI
                     LSVFIAVGMSVAGF"
     gene            368..679
                     /gene="traL"
                     /locus_tag="ACHUN9_003738"
     CDS             368..679
                     /gene="traL"
                     /locus_tag="ACHUN9_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0697030.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            701..883
                     /locus_tag="ACHUN9_003739"
                     /pseudo
     CDS             701..883
                     /locus_tag="ACHUN9_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            885..2135
                     /locus_tag="ACHUN9_003740"
                     /pseudo
     CDS             885..2135
                     /locus_tag="ACHUN9_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2132..2518
                     /gene="trbI"
                     /locus_tag="ACHUN9_003741"
     CDS             2132..2518
                     /gene="trbI"
                     /locus_tag="ACHUN9_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0697031.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2515..2826
                     /locus_tag="ACHUN9_003742"
                     /pseudo
     CDS             2515..2826
                     /locus_tag="ACHUN9_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            2821..3819
                     /locus_tag="ACHUN9_003743"
                     /pseudo
     CDS             2821..3819
                     /locus_tag="ACHUN9_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            3839..4586
                     /gene="traX"
                     /locus_tag="ACHUN9_003744"
                     /pseudo
     CDS             3839..4586
                     /gene="traX"
                     /locus_tag="ACHUN9_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4641..5201
                     /locus_tag="ACHUN9_003745"
     CDS             4641..5201
                     /locus_tag="ACHUN9_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0697032.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTLRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5331..5543
                     /locus_tag="ACHUN9_003746"
     CDS             5331..5543
                     /locus_tag="ACHUN9_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0697033.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1380 a         1420 c         1792 g         1271 t
ORIGIN      
        1 cgttaagtgt tcagggtact tctgcgcccg tcaaaaagaa atcgtttttt tctaaattca
       61 ctcgtctgaa tatgcttcgc ctggttcgtg cagtgatccc ggttgctgtt ctgatgatgt
      121 tgttccctga gttagctatg gctgctggta aaggcacgga cctgatggct aaaggtcagg
      181 aaacggttaa ggcgaccttc ggtaaggact ccagtatcgt taaatgggtt gttctggctg
      241 aagttctggt cggtgcagtc atgtacatga tgaccaaaaa cgtcaagttc ctggtcggct
      301 ttgccatact ctctgtattt attgcagtag gtatgtctgt tgccggattc tgacaggaaa
      361 taaaacgatg ttgggagacg agaataaact taagaaatat cgtttcccgg aaacactgac
      421 caaccagagc cgctggtttg gcctgccact ggatgaactg atccccgcag caatctgtgt
      481 tggctggggt atcacaacat cgaaatatct gttcggtatt ggtgcagcgg ttctggttta
      541 tttcgggatt aaaaaactga aaaaagggcg gggcagttcc tggttacgtg acctgattta
      601 ctggtatatg ccaacagccc tgctgcgcgg tatttttcat aatgttcccg attcgtgttt
      661 ccggcagtgg attaaataga actgatacca ggattgttat atggaacacg gtgcccgttt
      721 aagtaccagt cgtgtaatgg ccatcgcctt tatatttatg tcagtgctta ttgttctcag
      781 cctctctgtt aacctcattc agggggtgaa taactaccgt cttcagaatg agcaacgcac
      841 tgccgtgacc ccaatgggat ttaatgtgcc ctttgccgtg tcacacctac cgtaaccagc
      901 tggcgtttat cgatattttc ttccggggaa tgaataacac caactacaac atggcggtct
      961 gtggcacctc cggggccggt aaaaccgggc tgatacagcc acttatccgc agcgtgctgg
     1021 actccggggg ctttgccgtg gtgttcgaca tgggggatgg ctacaagtcc ctgtgtgaga
     1081 acatgggggg cgtatatctg gacggtgaaa ccctgcgttt taacccgttt gcgaacatca
     1141 ccgatatcga ccagtcagcg gagcgtgtcc gtgaccagtt gtcagtgatg gccagcccca
     1201 acggtaacct ggatgaagtg catgaaggtc tgctgctgca ggcggtcagg gcctcctggc
     1261 tggccaaaga gaacagggca cgtattgatg acgtggtgga tttcctgaaa aacgccagtg
     1321 acagcgagca gtatgccggg tcaccgacta tccgcagccg tctggacgaa atgattgtgc
     1381 tgcttgacca gtacactgcc aacggcactt acggccagta ttttaactct gatgagccgt
     1441 ccctgcggga tgacgcaaaa atggtggtgc tggagctggg cggactggaa gaccgtccgt
     1501 cactgctggt tgcggtgatg ttctccctga ttatctacat cgagaacagg atgtaccgca
     1561 cgccgcgtaa cctcaagaaa ctgaacgtca ttgatgaagg ctggcgtctg ctggacttca
     1621 aaaatcacaa ggtcggtgag tttattgaga aaggttaccg taccgcccgt cgtcataccg
     1681 gtgcttatat caccatcaca cagaacatcg tcgactttga ctctgacaag gcctccagtg
     1741 cggcccgtgc ggcatggggt aactcctcct acaaaattat cctcaaacag agtgcgaagg
     1801 agttcgcaaa atacaaccag ctgtatccgg accagttcca gccactgcag cgcgacatga
     1861 ttggtaagtt tggtgcggcc aaagaccagt ggttcagttc cttcctgctg caggtggaaa
     1921 accattcctc ctggcaccgc ctgtttgtgg acccgttaag ccgcgccatg tacagctctg
     1981 acggcccgga ttttgagttt gtgcagcaga aacgtaaaga ggggctgagt attcatgagg
     2041 cggtgtggca gctggcgtgg aagaagtcag ggccggagat ggcttcgctg gaagcctggc
     2101 tggaagaaca tgagaaatac aggagtgttg catgaccaca acgcaaaaaa cgactgatgt
     2161 tacagccccc cgacgcagcc actggtggtg gacggtgccg ggatgcctgg caatggtgtt
     2221 actgaacgca gccgtcagtt acggcattgt cagactgaac gcaccgatga ctgccgcctt
     2281 caacatgaaa cagactgtgg atgcattttt tgacagcgcc agtcagaaac aactgtcaga
     2341 agcacagtca aaagcccttt cagcgcgttt taacacggca ctggaagcca gcctgcaggc
     2401 gtggcagcag aaacaccacg cggtcattct ggtgtcgcct gccgtggtac agggggctcc
     2461 ggatatcacc cgggaaatcc agcaggatat tgcccggcga atgagagcgg aaccatgaga
     2521 tgccgggggc tgattgccct gctgatatgg gggcagagtg tggccgccgc cgatcttggc
     2581 acctggggtg atctgtggcc ggtaaaggag ccggacatgc tgacggtgat catgcagcgc
     2641 ctgacggcac tggagcagtc cggtgagatg ggccggaaaa tggatgcctt taaggagcgg
     2701 gtgatccgca acagtctgcg gccccctgcc gtgcccggta tcggacgcac ggagaaatac
     2761 agcagccggt tgtttgaccc gtccgtcaga ctggctgcgg atatccggga taacgaaggg
     2821 cgggtggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag
     2881 gtgtacaccg ataaccgtca gggctggacg gatgccatca acaatgccgt acagaaagga
     2941 actgcccacg atgtgtttga gccgaaaccg gaccgggagg tcatgaatgc agagcggctg
     3001 ttcagtacgg cgcgggagct gcgggacgtg gcggcaggcc gtgccgttct ccgtcaggcg
     3061 gggctggccg ggggagacag tcctgcacgg tttattgctc cgggacgtaa atatccgcaa
     3121 ccgtatgtgg cactgccggc gtttgaccgt aacggcaagt ccgccggtat ctggctgaac
     3181 ccgctgacca cggatgacgg aaacgggctg cggggattca gcggtgaagg tcgggtaaaa
     3241 ggcagcgggg atgcgcagtt cgtggccctg cagggcagcc gtaacggaga aagcctgctg
     3301 gctgataata tgcaggatgg cgtccggatt gcccgtgata atcctgacag tggtgtggtg
     3361 gtaagcattg ccggtgaagg ccgtccgtgg aatcccggtg ccatcaccga tggccgcgtg
     3421 tggggggata tcccggacaa cagcgtccag ccgggagccg gaaatggcga accggtcacg
     3481 gcagaggtac tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgtcgc
     3541 gcagatgaaa ttgtccgtaa aatggcagag aacaaacctg acctgccgga cggcaaaaca
     3601 gagctggctg tcagggaaat tgccgggcag gagcgtgacc gggctgccat aactgaacgg
     3661 gaagccgcgc tgccggagag tgtgctgcgt gaatcacaac gggagcggga agcggtccgg
     3721 gaggttgccc gggaaaatct gctgcaggag cgtctgcagc agatggagcg ggatatggtt
     3781 cgtgacctgc agaaagagaa aaccccgggc ggagactgat acaggaagac aaacactgat
     3841 gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga ccgatacctg
     3901 gttgcagtca tttctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcact
     3961 ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg aatggatgtt
     4021 tctggccggg cgaggagcct ttccgctttt tgccctggtg tgggggctga atctgtcccg
     4081 tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa ttattgccca
     4141 gttcgcatat tatctggcag gctttccctg gtatgagggg aatatcctgt ttgcctttgc
     4201 agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc
     4261 cattcttctg atggccctgt tgggggcctt tgtccggcac cagttacggc attgccgggc
     4321 tgctgatgct ggcagtcagc caccgactgt accgggcgga agacagagcg gaacgtctgg
     4381 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     4441 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     4501 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     4561 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     4621 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     4681 aaacggaagg ggagacgcct gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     4741 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcagagctgg
     4801 cggcaaaaaa agcgcaggcc agacaggcac tgtccattta tctgaacctg cccacgctgg
     4861 atgaggccgt gaataccctg aaaccctggt ggccgggatt atttgacggt gacaccctcc
     4921 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     4981 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     5041 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     5101 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaacc
     5161 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     5221 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg cttccgaaag gaagccagtc
     5281 cactgaatta tttatcacaa taatactgac aactaaaggg ggacaagaaa atgaatggat
     5341 tcagaaacag ttcacggaac ggccaggtct ggcgttacca gcgtgccggc gggcgggccg
     5401 ttattctgga agtcagtgga cgctggatgg aagcggcaga agcatggcga cgggctgcct
     5461 gtgttgcgcc caggacagac tggcagcagt ttgcccgaaa aagggctgaa cactgccacc
     5521 ggcgctgccg gggaagggtg taacgaaaaa agcagccttc attactccag gagtcagaag
     5581 gctgaaagga gtttatgaga tttgcttttt ttaatatccg gtcgggcagt atgaccggac
     5641 agttctgagt agcacagagt tttttccgta ttaagtcgtg attttatatt ttgtgattaa
     5701 tttcacaaaa taaggtgtta ttcagtgtgt gctgcaatat tcaggatgcc tgaaatacag
     5761 atgctattta tctgatatgg agaataacat gaggaaatag taaggcagta tgactttccg
     5821 tgagacagat gctggccaac gtgggatatt taacgcgggt taa
//
LOCUS       DAZOYS010000224         5725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOYS010000224 DAZOYS010000000
VERSION     DAZOYS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.225"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..861
                     /gene="yneK"
                     /locus_tag="ACHUN9_003747"
     CDS             <1..861
                     /gene="yneK"
                     /locus_tag="ACHUN9_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0697034.1"
                     /translation="QFKLTYPVKNGRHKENIEFQVVINLSPIYLDNFRHDGEINIFCA
                     PNPKPVTMGRVFQTGVERVLFLFLNDFIEQFPMFNPGVPIKIAHTPHIEPLPPDHHTA
                     ADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITAILDITDSELKED
                     LLQSLMDSLGSNKHVLPEVCICFLFLLAEQESPHFQDLFLFFANMLLHYHQFMNPNES
                     DLNDVLMPASLSDDKIIKHMARRTLELFVKNETPPKVTHEDLVKNRPRSPVRPPIPAT
                     SKTPDLPERH"
     gene            1011..2201
                     /gene="ydeA"
                     /locus_tag="ACHUN9_003748"
     CDS             1011..2201
                     /gene="ydeA"
                     /locus_tag="ACHUN9_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0697035.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2226..2891)
                     /gene="marC"
                     /locus_tag="ACHUN9_003749"
     CDS             complement(2226..2891)
                     /gene="marC"
                     /locus_tag="ACHUN9_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0697036.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3103..3537
                     /gene="marR"
                     /locus_tag="ACHUN9_003750"
     CDS             3103..3537
                     /gene="marR"
                     /locus_tag="ACHUN9_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0697037.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3557..3940
                     /gene="marA"
                     /locus_tag="ACHUN9_003751"
     CDS             3557..3940
                     /gene="marA"
                     /locus_tag="ACHUN9_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0697038.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3972..4190
                     /gene="marB"
                     /locus_tag="ACHUN9_003752"
     CDS             3972..4190
                     /gene="marB"
                     /locus_tag="ACHUN9_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0697039.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4247..5686)
                     /locus_tag="ACHUN9_003753"
     CDS             complement(4247..5686)
                     /locus_tag="ACHUN9_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0697040.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1506 a         1409 c         1261 g         1549 t
ORIGIN      
        1 cagtttaaac tcacttatcc cgtcaagaat ggaagacaca aagaaaatat tgaatttcag
       61 gtagtaataa atttaagccc tatttattta gataattttc gccatgatgg agaaattaat
      121 attttttgcg ctcccaaccc aaagcctgtc actatggggc gcgtatttca gaccggcgtc
      181 gagcgtgtgc tctttctttt tctgaatgat tttattgaac aatttccaat gttcaaccct
      241 ggcgttccga tcaaaatagc gcatacacca catattgaac ccctgcctcc ggatcaccat
      301 accgctgcag attacttacg ccagtttgat ttgcttgtcc tgaattttat ctctcgcggt
      361 aattttgtca tactcccccg attatggaat aactctgagg ttcacagatg gttcgtcaat
      421 aaagatccta acttgatcac cgccattctc gacataacgg acagcgaatt aaaagaggat
      481 ttattgcaaa gcctgatgga ttcattaggt tctaacaaac atgtactacc cgaagtctgc
      541 atctgctttt tattcctttt agcagaacaa gaatctcctc attttcagga cttgttttta
      601 tttttcgcca atatgttatt gcactatcac caatttatga atcccaatga aagtgatttg
      661 aatgacgtgt tgatgccagc atcattaagt gatgataaaa ttatcaagca tatggcgcgc
      721 aggaccctcg aactgtttgt aaaaaatgaa acaccgccaa aagttactca cgaagacctg
      781 gtgaaaaaca ggcctcgctc ccctgtcaga ccacctatac ccgcaacctc caaaacgcca
      841 gacctccctg aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg aacagaatca
      901 gcgtcagaga aacggaaaac gcgatccaga tcacaaatac attgtattca catcattaac
      961 cgttttaaga tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa
     1021 acactgtttc ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca
     1081 tcttcaacac caccgaattt gtccctgttg gcctgctctc tgacattgcg caaagttttc
     1141 acatgcaaac cgctcaggtc ggcatcatgt tgaccattta cgcatgggta gtagcgctaa
     1201 tgtcattgcc ttttatgtta atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc
     1261 tgtttgtggt gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc
     1321 tggtgatcag tcgcattggt gtggcttttg cacatgcgat tttctggtcg attacggcgt
     1381 ctctggcgat ccgtatggct ccggccggga agcgggcaca ggccttgagt ttaattgcca
     1441 ccggtacagc actggcgatg gtcttaggtt tacctctcgg gcgcattgtg gggcagtatt
     1501 tcggttggcg aatgaccttc ttcgcgattg gtattggggc gcttatcacc cttttgtgcc
     1561 tgattaagtt acttccctta ctgcccagtg agcattccgg ttcattgaaa agcctcccgc
     1621 tattgttccg ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg
     1681 cccattacac ggcatacagc tatatcgagc cttttgtgca aaacattgcg ggattcagcg
     1741 ccaactttgc cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga
     1801 ttttcggtaa actgggtaat cagtatgcgt ctgcgttggt gagtacggcg attgcgctgt
     1861 tgctggtgtg tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc
     1921 tgagtatttt ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc
     1981 tggcgctggc accagatgct accgacgtcg cgatggcgct attctccggc atatttaata
     2041 ttggaatcgg ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg tcaatgtcga
     2101 tgattggtta tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat
     2161 ttcgccgctg gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc
     2221 cttttttaat ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc
     2281 atacatacca gcaggaatcc catcagacgg gagatcgctt caatgccacc cttgcccacc
     2341 agccgcataa ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa
     2401 aagatcagcg gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact
     2461 gtggacgccg agctaataat catcgctatg gttcccggac cagcggtact tggcattgct
     2521 agcggcacaa aggcaatatt ggcactgggt tcatcttcca gctcttccga cttgcttttc
     2581 gcctccggtg aatcaatcgc tttctgttgc ggaaagagca tccgaaaacc gataaacgcg
     2641 acgattaagc cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc
     2701 agttgcccgg cgtaatacgc caccatcatg atggcaaata cgtacaccga ggccatcaac
     2761 gactgacgat tacgttcggc actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca
     2821 gttgttaatg ggttagctaa cggcagcaac accaccagcc ccaggccaat tgctttaaac
     2881 aaatctaaca ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg
     2941 gtaaacaagg ataaagtgtc actctttagc taaccttgca tcgcattgac caaaacttga
     3001 accgatttag caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctgggc
     3061 aatattatcc tctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat
     3121 ctgttcaatg aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat
     3181 cgtctgctta acgagtatct gtctccgctg gatattaccg cggcacagtt taaggtgctc
     3241 tgctctatcc gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc
     3301 gacctgggag cactgacccg tatgctggat cgcctggtct gtaaaggctg ggttgaaagg
     3361 ttgccgaacc cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca
     3421 atatgcgaac aatgccatca attagttggc caggacctgc atcaagaatt aacaaaaaac
     3481 ctgacggcgg acgaagttgc aacacttgag catttgctta agaaagtcct gccgtaaaca
     3541 aaaaagaggt atgacgatgt ccagacgcaa tactgacgct attaccattc atagcatttt
     3601 ggactggatc gaggacaacc tggaatcgcc actgtcactg gagaaagtgt cagagcgttc
     3661 gggttactcc aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc attcattagg
     3721 ccaatacatc cgcagccgta agatgacgga aatcgcgcaa aagctgaagg aaagtaacga
     3781 gccgatactc tatctggcag aacgatatgg cttcgagtcg caacaaactc tgacccgaac
     3841 cttcaaaaat tactttgatg ttccgccgca taaataccgg atgaccaata tgcagggcga
     3901 atcgcgcttt ttacatccat taaatcatta caatagctag ttgataacgt gacaacgtca
     3961 ctgaggcaat catgaaacca ctttcatccg caatagcagc tgcgcttatt ctcttttccg
     4021 cgcagggcgt tgcggaacaa accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg
     4081 ttgttccccc atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt actggcagtg
     4141 ataagtcgga tgcgctcggc gtgccctatt ataatcaaca cgctatgtag ttattgtcag
     4201 gctctgatca cagagcctga catctccgta atatcttcct caagtctcaa atattttcgc
     4261 cattgttggc gatgagatcc cgataccagt aaaacgacgc tttacgtatc cgttccagcg
     4321 ttcccttccc ttcgttgtct ttatcgacat aaatcattcc gtagcgcttt ttcatttccc
     4381 ctgttccggc agaaaccagg tcaatgcagc cccacggggt gtagccaatt aagtcaacac
     4441 catcttcaac tacggctttt ttcatttccc gaatatggga agcaaagtaa tcaatgcgat
     4501 agtgatcgtt caccgtgccg tcagcttgtc tctggtcaac cgcaccaaat ccattttcga
     4561 caataaacag cggcaactgg aaatgatccc agaaccagtt gagtgaataa cgtagccctg
     4621 ccggatcaat ttgccatccc cattcggatg tatcgatata agggttgctg accaaatctc
     4681 gtggttcaac ataatccagt tgcgggttat cgggagaaaa ttgtgtcgta aaagacatgt
     4741 agtagctaaa accgataaag tcgacacatc cactggcaag aatcgcgtta tcttctggtg
     4801 tgatatcgag gttgaatcct ttcctggcaa agtaattcag catatgttgc ggataatatc
     4861 cacgagcatg aacatcagta aaccagtaac gacgatgcat cgctttcgtg gccatcatca
     4921 tatcgttggg tgcacacgtc agaggataga tggggcacat ggcgatcata cagccaatgt
     4981 taaattcagg attgatcgat tttccagtct gtaccgccag ggcactggca actaactcgt
     5041 aatgtaccgc ctggtacatt atttgttcgc gctcattgat atcttcctct ggcgaataca
     5101 agataccgga attagtaaat ggacacaggc tttcgctgaa attcacctga ttattgattt
     5161 cgttaaacgt catccagtac tttacttttt ctttatagcg cgtgaagacc gttgatgcga
     5221 agcggataaa aaagtcgatc agcttacggt ttcgccagcc accatatttt gtcaccagat
     5281 gataaggcat ctcaaaatgc gaaagcgtca ccacaggttc cattccttgc ttcaggcatt
     5341 catcgaacag atcatcataa aattgtaaac cctcttcatt cggctcctgt tcgtcacctt
     5401 gcggaaagat tcgtgtccag gcaatggaag ttcgaaagca tttgaatccc atctcggcaa
     5461 ataactgaat atctgtttta tagcgatgat aaaaatcaat tgcttcatga ttgggataat
     5521 taagcccgtc gataacgcct tctgtcactt cacgcggcac cccgtgagcg ccagcagtca
     5581 tcacatcagc gatactgatg ccttttcctc cttcattcca gccaccttcc aactgatgcg
     5641 cggctaccgc gccaccccat aaaaaacctt ttttaaatcc tgacatacac aactccttaa
     5701 ctaaaataga ttaccaccag acttc
//
LOCUS       DAZOYS010000225         5682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOYS010000225 DAZOYS010000000
VERSION     DAZOYS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.226"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(78..197)
                     /locus_tag="ACHUN9_003754"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="ACHUN9_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHUN9_003755"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHUN9_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0697041.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHUN9_003756"
     CDS             complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHUN9_003756"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0697042.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHUN9_003757"
     CDS             complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHUN9_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0697043.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHUN9_003758"
     CDS             complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHUN9_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0697044.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHUN9_003759"
     CDS             complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHUN9_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0697045.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5433..>5682)
                     /locus_tag="ACHUN9_003760"
     CDS             complement(5433..>5682)
                     /locus_tag="ACHUN9_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697046.1"
                     /translation="IARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQ
                     QTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1479 a         1445 c         1403 g         1355 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtgtaaattt aaattatttt aacctgattc ttgcaatgtt tttacattgc
     2221 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     2281 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     2341 attcacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     2401 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     2461 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     2521 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     2581 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     2641 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     2701 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     2761 cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     2821 tttgatatag gttttaaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     2881 caccgggtaa agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     2941 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     3001 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     3061 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt
     3121 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt
     3181 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
     3241 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     3301 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     3361 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     3421 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
     3481 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     3541 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     3601 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     3661 aacagaggac aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga
     3721 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     3781 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     3841 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     3901 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     3961 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     4021 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     4081 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     4141 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgta
     4201 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     4261 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     4321 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     4381 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     4441 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     4501 actaataagc ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     4561 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     4621 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     4681 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     4741 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     4801 tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat
     4861 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca
     4921 ccaccactgg cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg
     4981 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
     5041 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
     5101 tgtttagcga tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg
     5161 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
     5221 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agtactaacc
     5281 gatgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
     5341 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
     5401 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg
     5461 gcggcttttt tggcctgagc gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca
     5521 acctgttctt ctggttctgc gttagtctgt tgctgttcca gcttgcgcgc tttggcacgg
     5581 gcaatagcgg cttcgacggc ggctttgcgc ggatcaacct gttcttctgg ttccgcatta
     5641 gcctgttgct gttccagttt gcgcgctttg gctcgggcaa ta
//
LOCUS       DAZOYS010000226         5666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOYS010000226 DAZOYS010000000
VERSION     DAZOYS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.227"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHUN9_003761"
     CDS             complement(<1..661)
                     /locus_tag="ACHUN9_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0697047.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHUN9_003762"
     CDS             761..1351
                     /locus_tag="ACHUN9_003762"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR0697048.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUN9_003763"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUN9_003763"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0697049.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUN9_003764"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUN9_003764"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0697050.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUN9_003765"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUN9_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0697051.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1460 a         1335 c         1410 g         1461 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcat
//
LOCUS       DAZOYS010000227         5641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOYS010000227 DAZOYS010000000
VERSION     DAZOYS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.228"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..793
                     /gene="lpfD-O113"
                     /locus_tag="ACHUN9_003766"
     CDS             <1..793
                     /gene="lpfD-O113"
                     /locus_tag="ACHUN9_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0697052.1"
                     /translation="LTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNP
                     SGNTKYNCNRIEPLSISSGGKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDY
                     SQPMADVYIRGDITAPQSCEINNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTI
                     ECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTD
                     MGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1041..2081
                     /gene="pstS"
                     /locus_tag="ACHUN9_003767"
     CDS             1041..2081
                     /gene="pstS"
                     /locus_tag="ACHUN9_003767"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0697053.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2168..3127
                     /gene="pstC"
                     /locus_tag="ACHUN9_003768"
     CDS             2168..3127
                     /gene="pstC"
                     /locus_tag="ACHUN9_003768"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0697054.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3127..4017
                     /gene="pstA"
                     /locus_tag="ACHUN9_003769"
     CDS             3127..4017
                     /gene="pstA"
                     /locus_tag="ACHUN9_003769"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0697055.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4108..4881
                     /gene="pstB"
                     /locus_tag="ACHUN9_003770"
     CDS             4108..4881
                     /gene="pstB"
                     /locus_tag="ACHUN9_003770"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0697056.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            4896..5621
                     /gene="phoU"
                     /locus_tag="ACHUN9_003771"
     CDS             4896..5621
                     /gene="phoU"
                     /locus_tag="ACHUN9_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0697057.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1386 a         1357 c         1487 g         1411 t
ORIGIN      
        1 attaacttat gctggggttc actctaatat taattattat gatcttaatc cgaaccttga
       61 tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt ttgaatatca
      121 tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac ctttaagtat
      181 atccagtggg ggtaaagcga tagtatattt ttatatcaag aagacatttg caggaaaatt
      241 gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg acaccccggt
      301 ggattactca cagccgatgg ctgatgttta tattcggggc gatattacag ctccgcaaag
      361 ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac cagctgcaga
      421 tttttcatct gttgttggaa gtgcggtaac aacacataaa attactaaaa cagtcactat
      481 tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt tttatgctac
      541 cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg ggataaaaat
      601 ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa cagacatggg
      661 taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact ttttcagctg ctcctgcgag
      721 ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga taactgttga
      781 gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat ctcacccatt
      841 tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt tcatcttcac
      901 atttaaaatc agtaagttat ttgttaattc ctttctgtca taaaaatgtc atattcctta
      961 catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac aatgctttat
     1021 gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg
     1081 cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg
     1141 caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa gaaatcggta
     1201 ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt atcgctaata
     1261 ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct caggaaggtc
     1321 tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt ccagggctga
     1381 agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg ggcaaaatca
     1441 agaagtggga tgatgaaacc atcgccaaac tgaatccggg tctgaaactg ccttcacaaa
     1501 acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc accagctacc
     1561 tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc gtaaaatggc
     1621 cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag cgtctgccgg
     1681 gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg tacaccaaac
     1741 tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct aatgcagcaa
     1801 aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa ggcgaagatg
     1861 catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag aaaccagaac
     1921 aaggcacaga agtgctgaaa ttcttcgact gggcgtacaa aaccggggct aaacaggcga
     1981 acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc gctgcgtgga
     2041 agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactct aggccgggta
     2101 cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt taactgaaga
     2161 gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg gcgacataat
     2221 tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg gtggcattat
     2281 tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg ctttcctatg
     2341 gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga tctacggtac
     2401 gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg gtatcgccct
     2461 gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg ccattgagct
     2521 gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct ttgcgccgct
     2581 gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc cgattgttgg
     2641 cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg tgatcctcgc
     2701 catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac aaaccccggt
     2761 gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta tctggcgtat
     2821 cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc tgggccgcgc
     2881 gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc tcgacagcgc
     2941 ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat ttgcggaagc
     3001 ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt ttgtgattac
     3061 cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga atgagggggc
     3121 acgctaatgg ctatggttga aatgcaaacc actgcggcgc tggctgaatc tcgccgcaaa
     3181 atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat ggcgacgatg
     3241 gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg cggtatcgac
     3301 ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga aggtggtggt
     3361 ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt attcggtacg
     3421 ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc ctggctggca
     3481 gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt ggttggtctg
     3541 tttgtctaca ccatcgtggt ggcacagatg gagcacttct ccggctgggc gggcgtaatt
     3601 gcgctggcgc tgttgcaggt gccgatcgtt atccgcacca ccgagaacat gctgaaactg
     3661 gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg gaagatgatc
     3721 tccgcgatta cgctgaaagc gtcggtgtcc gggattatga ccggtatcct gctggcgatt
     3781 gcccgtattg ccggtgaaac cgcgccgctg ctgtttaccg cgctctccaa ccagttctgg
     3841 agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa gtttgcgatg
     3901 agcccgtttg cggaatggca acaactggcc tgggccgggg tattgatcat taccctgtgc
     3961 gtcctgctgc tgaacattct ggcgcgcgtt gtttttgcga agaataaaca cggttaacac
     4021 tttgccggat gcggcgtgaa cgccttaccc ggcctacggt aagcctgatt agcgaaaagc
     4081 atcaggtacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc gagtaaaatt
     4141 caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa catcaacctg
     4201 gatatcgcta aaaaccaggt aacggcgttt atcgggccgt ccggctgcgg taaatcgacg
     4261 ctgctgcgta ccttcaacaa aatgtttgaa ctgtatccgg agcagcgcgc agaaggtgaa
     4321 attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact gctgcgtgcg
     4381 aaagtgggca tggtgttcca gaaaccgacg ccgttcccga tgtccatcta cgacaacatc
     4441 gcttttggtg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga gcgcgtgcag
     4501 tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca ccagagcggt
     4561 tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat cgccattcgc
     4621 ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accccatctc taccgggcgt
     4681 attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt tacccacaac
     4741 atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg cgaattgatt
     4801 gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac agaagactac
     4861 atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat cttaataaac
     4921 atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg atgaccatgg
     4981 gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac caggacagcg
     5041 atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa gtggcgatcg
     5101 atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac ctgcgactgg
     5161 ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg gcggacaaaa
     5221 tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg gtaagtctgg
     5281 agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg ttcgcgcgga
     5341 tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat caggaatacg
     5401 aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc attccgagcg
     5461 tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc tgccagaata
     5521 tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta ggcggtgatg
     5581 agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga caaatcccaa
     5641 t
//
LOCUS       DAZOYS010000228         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOYS010000228 DAZOYS010000000
VERSION     DAZOYS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.229"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUN9_003772"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUN9_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0697058.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUN9_003773"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUN9_003773"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0697059.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUN9_003774"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUN9_003774"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0697060.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUN9_003775"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUN9_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0697061.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZOYS010000229         5631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOYS010000229 DAZOYS010000000
VERSION     DAZOYS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.230"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHUN9_003776"
     CDS             <1..706
                     /locus_tag="ACHUN9_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0697062.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACHUN9_003777"
     CDS             complement(1909..2100)
                     /locus_tag="ACHUN9_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0697063.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACHUN9_003778"
     CDS             2165..2488
                     /locus_tag="ACHUN9_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0697064.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACHUN9_003779"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACHUN9_003779"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACHUN9_003780"
     CDS             3166..3696
                     /locus_tag="ACHUN9_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0697065.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="ACHUN9_003781"
     CDS             3703..3936
                     /locus_tag="ACHUN9_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0697066.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="ACHUN9_003782"
     CDS             4417..5469
                     /locus_tag="ACHUN9_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697067.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5483..>5631
                     /locus_tag="ACHUN9_003783"
     CDS             5483..>5631
                     /locus_tag="ACHUN9_003783"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFR0697068.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1484 a         1155 c         1515 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat accatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAZOYS010000230         5546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOYS010000230 DAZOYS010000000
VERSION     DAZOYS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.231"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..165
                     /locus_tag="ACHUN9_003784"
     CDS             <1..165
                     /locus_tag="ACHUN9_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697069.1"
                     /translation="SEEFLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEY
                     TYDYDLLNAI"
     gene            complement(779..1155)
                     /gene="rnpB"
                     /locus_tag="ACHUN9_003785"
     ncRNA           complement(779..1155)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUN9_003785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1188..2333)
                     /gene="garK"
                     /locus_tag="ACHUN9_003786"
     CDS             complement(1188..2333)
                     /gene="garK"
                     /locus_tag="ACHUN9_003786"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0697070.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2430..3320)
                     /gene="garR"
                     /locus_tag="ACHUN9_003787"
     CDS             complement(2430..3320)
                     /gene="garR"
                     /locus_tag="ACHUN9_003787"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0697071.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3350..4120)
                     /gene="garL"
                     /locus_tag="ACHUN9_003788"
     CDS             complement(3350..4120)
                     /gene="garL"
                     /locus_tag="ACHUN9_003788"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0697072.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4136..5470)
                     /gene="garP"
                     /locus_tag="ACHUN9_003789"
     CDS             complement(4136..5470)
                     /gene="garP"
                     /locus_tag="ACHUN9_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0697073.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1374 a         1561 c         1293 g         1318 t
ORIGIN      
        1 tcagaggaat tcctttttga taatagcctc gagaaagacg aaaattcttc ggacaattgc
       61 tttgaggtcg aaaaatataa cgcatatgaa gcatataact cagagaatct atattttacc
      121 agagaggaat atacgtatga ttacgacctt ttaaatgcaa tatagacata ataacttctt
      181 acaaaatcta taattaatac cgtagatatt cattcatctt accctgtgca ccaaaagcaa
      241 aaactcactg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga tgcgctacgc
      301 ttatcaggcc tacgccatct ctacaatata ttgaatttgc gtgcttttgt aggcaggata
      361 aggcgttcac gcagcatccg gcatgaataa agcgcactca acaatctgaa acccgccgga
      421 acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt tgggtatagt
      481 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
      541 tttacgtaaa tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agcgtcgtgg
      601 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg ttttttacgt
      661 aaaccaggtg aaactgaccg ataagccgct ttcttttggg tatagcgtcg tggacagtca
      721 ttcatctttc cgctcctcca aaagcaaaaa cccgccgaag cgggttttta cgtaaatcag
      781 gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag gccagcaatc
      841 gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat gaacccctat
      901 ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg cgcggtgcgc
      961 tcttaccgca ccctttcacc cttacctgat cccgcttgcg cgggccatcg gcggtttgct
     1021 ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct gccctatgga
     1081 gcccggactt tcctcccctc cgcccgtctc ccccgaagag gacgacgacg aagcggcgac
     1141 tgtctggtca gcttcggcgc gcagtataga gggtttgcgc gcccttgtca ccccgcgttg
     1201 cgcattccaa tcgccagtgt cgcggcgata ttacgtgaag cacggcagat attgtcataa
     1261 gccccgcgga atgcttcgtc caacgtacct atgctggtca atacgctgaa gaccgcatcg
     1321 atgccatgct gatgtacaac gccaacatca tcggtcaggc tacccgcaat gccaatcacc
     1381 ggtttatggt acttcttcgc cacgtttgca acgccaatcg gtaccttgcc gtgaatactc
     1441 tggctgtcaa tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcttcc
     1501 agattcagcg ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc
     1561 atcagcgccg cgcccatacc gcccgcagct cctgcgccgg ggacatcttt cacatcaaca
     1621 tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc
     1681 atcgcttcac tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc
     1741 agcggattgg tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg
     1801 ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg
     1861 ccgttggcgt cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt
     1921 gtagcgctgc cgccaatgcc gataataatg tttgttgcac tgctctccag cgcctgcagg
     1981 attaactcgc ctgtgccgcg tgaagtggtt acgagtggat cgcgtttttc cgcaggtacc
     2041 agctccagcc cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc
     2101 ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg acgttcagcc
     2161 ccctgggtgg ctgcaatcat cgcttccacc gttccttcac caccgtcggc aaccggaaca
     2221 gaaacatact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc
     2281 tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt catacctatg
     2341 cctgttacca catgacgccg gagggcgttt ctcttattcg gcctagattc caggcccgga
     2401 ttgcaataca ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt
     2461 tcgtagtagc acgccagggc gctatgatcc gccgttccta aaccatctgc acgcagtgcc
     2521 tgcatcatct ccataaccgc agctgtgagc agcagttgtg cgccgacgcc gtgagaagta
     2581 tccagcgcat tcgccagatc cttaatatgc agatcaatgc ggaagcccgg cttgaagttg
     2641 cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag cccaccgcga
     2701 attgcctgat aaaccaggtc cgggttaacg cccgctttgg ttgccagcgt taacgcttct
     2761 gacatcgcag caatattcag cgccacaatg acctgatttg ccagtttggt gacgttacct
     2821 gcaccgattt ccccggtatg caccacggaa cccgccatcg ctttcatcaa atcatagtat
     2881 ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc
     2941 ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg
     3001 ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc
     3061 ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg aggggagttt
     3121 ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agacgctgtt
     3181 tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc
     3241 gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc caggccaata
     3301 aaaccaactt tcatagtcat atcaaccatc tctcttgttg cggtggtggt tattttttaa
     3361 aggtatcagc cagtttctga gtggcagagc ggaagacgcc aagatcgctg ccgacagcca
     3421 caaacgtcgc gccccattcc agataacgac gcgcatcggc ttcgaccggc gcgagaatac
     3481 cgctgggttt gccgtgcgcg ctggcacggt taaaaatatg ctgaattgct ttttgtacat
     3541 ccggatgcga ggcattgccg agatggccca atgccgcagc cagatcgctg gggccgacga
     3601 agataccgtc tacaccttcg gtagcggcaa tagcatcgac gttatctacg ccctgctgac
     3661 tttctatctg gaccagaata gtaatgttct tgttcgactg agcgaaataa tccgccacgg
     3721 tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggcgggtaac
     3781 gagttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt
     3841 tatagaaacc gatatccaga agacgcttaa taattaccgg ctcgttggtc ggcactcgca
     3901 ctactggcgc gctggcgctg cctttcaggg ccattaactg cggaataaac gtggaaatat
     3961 cgtttggcgc atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt
     4021 cagtgctaat cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca
     4081 gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt
     4141 tctgcaattc catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca
     4201 gcgctgaaca tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt
     4261 agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc
     4321 cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac
     4381 caaatccttt gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg gtgttgttgg
     4441 tgtagttaca taagatgatg gtggaaacca gcaacattcc cagcacaatc ggtagcttac
     4501 gtgccagggt cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca
     4561 cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg
     4621 acatgccttt ttcctgcacc agataaatcg ggaaccaggt gaggaagaac caggtgatgg
     4681 tgttgataaa atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct
     4741 tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat
     4801 cgaccaccgc gccattttca gagataaact tcagctcttc cgcagacata cgtgggtgat
     4861 ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacca atccccccca
     4921 taacggtgaa gacgtgctcc cagccccaag cgaaagtcag ccagccaagc agcggcgaaa
     4981 agagcgccag cgagaaatat tgcgccgagt taaagatggc ggaggcagta ccacgttctt
     5041 tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatggc gcttccgaga
     5101 accccagcat aaagcgcata aagaacatgg agatccctgc ccaggccagc gggaacatat
     5161 caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt
     5221 ttttcgagcc aaacttatca agcaaccagc cgccggggat ttgcatcagc aagtaggccc
     5281 agccaaaagc agagaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca
     5341 cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg gtaacaataa
     5401 aaataatcag taatattaaa tagctggtat gcacgccttt cttttttacg tcaacggtgt
     5461 ccagaatcat tttatttacc tcgggtactt atgctgattt ttattattat ggggaaggtg
     5521 ttatttatga gttttattta tgccgt
//
LOCUS       DAZOYS010000231         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOYS010000231 DAZOYS010000000
VERSION     DAZOYS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.232"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHUN9_003790"
     CDS             complement(<1..1369)
                     /locus_tag="ACHUN9_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR0697074.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUN9_003791"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUN9_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0697075.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUN9_003792"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUN9_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0697076.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUN9_003793"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUN9_003793"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0697077.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUN9_003794"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUN9_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0697078.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZOYS010000232         5528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOYS010000232 DAZOYS010000000
VERSION     DAZOYS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.233"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            242..583
                     /gene="raiA"
                     /locus_tag="ACHUN9_003795"
     CDS             242..583
                     /gene="raiA"
                     /locus_tag="ACHUN9_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0697079.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    686..811
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            687..734
                     /gene="pheL"
                     /locus_tag="ACHUN9_003796"
     CDS             687..734
                     /gene="pheL"
                     /locus_tag="ACHUN9_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0697080.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHUN9_003797"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHUN9_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0697081.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHUN9_003798"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHUN9_003798"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0697082.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHUN9_003799"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHUN9_003799"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0697083.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="ACHUN9_003800"
     CDS             4448..4813
                     /locus_tag="ACHUN9_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0697084.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="ACHUN9_003801"
     CDS             4963..5481
                     /locus_tag="ACHUN9_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0697085.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1390 c         1241 g         1378 t
ORIGIN      
        1 ttgagctgcc gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga
       61 tctcatcaaa tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg
      121 atttctcaca aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac
      181 attttgacat caggaacggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt
      241 tatgacaatg aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt
      301 cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat
      361 tctgtccaaa gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt
      421 tctggttgcc agtggtaaac atgaagatat gtacactgca attaacgaat tgatcaacaa
      481 gctggaacgg cagctcaata aactgcagca caaaggtgaa gcacgtcgtg ccgcaacatc
      541 ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgg
      601 atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg
      661 tactaaagtc acttaaggaa acaaacatga aacacacacc gtttttcttc gcattctttt
      721 ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat
      781 aaggcctccc aaaccggggg gcctttttta ttgataacaa aaaaggcaac actatgacat
      841 cggaaaatcc attactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cattactggc agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca
     1441 ttaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atcctcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt
     1681 tggcacgtaa agccattaac atgtctgacc aggttccggc gaaaacgacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaattac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcggttgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatgacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcagc aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacaaacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttctttac acgaacaatt tgctcgcgct tttgatgctt
     4381 atttcgtaca aataaattta cacctccttt aaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga
     4741 aagtgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac gatcgcatga aaaaatgata ttttaatgag cattctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca ggagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgca
//
LOCUS       DAZOYS010000233         5407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOYS010000233 DAZOYS010000000
VERSION     DAZOYS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.234"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            76..1209
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUN9_003802"
     CDS             76..1209
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUN9_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0697086.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(1206..2021)
                     /gene="fepC"
                     /locus_tag="ACHUN9_003803"
     CDS             complement(1206..2021)
                     /gene="fepC"
                     /locus_tag="ACHUN9_003803"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0697087.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2018..3010)
                     /gene="fepG"
                     /locus_tag="ACHUN9_003804"
     CDS             complement(2018..3010)
                     /gene="fepG"
                     /locus_tag="ACHUN9_003804"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0697088.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3007..4011)
                     /gene="fepD"
                     /locus_tag="ACHUN9_003805"
     CDS             complement(3007..4011)
                     /gene="fepD"
                     /locus_tag="ACHUN9_003805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0697089.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4122..5372
                     /gene="entS"
                     /locus_tag="ACHUN9_003806"
     CDS             4122..5372
                     /gene="entS"
                     /locus_tag="ACHUN9_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0697090.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
BASE COUNT         1265 a         1452 c         1488 g         1202 t
ORIGIN      
        1 gataattctc accaactggt aaagtgagcg ccgtggagat tttcccctat tggactcatt
       61 ttcaggttat accccatgtc atcattgaat attaaacagg gaagtgacgc tcatttttct
      121 gagtatcctc tggcgtcgcc cagtaataat gaaattgatt tacttaatct aatcgaagtt
      181 ttatggcggg ccaaaaaaac ggtcatggcg gtcgtttttg catttgcctg cgcaggttta
      241 ctgatctctt tcatcctgcc gcaaaaatgg accagctcag ccgttattac tcccgccgaa
      301 gccattcagt ggcaggacct ggagaaaacc tttaccaaac tccgcgttct tgatctcgat
      361 gttaatatcg accgtggcgg tgcatttaac ctgtttatca agaagtttca gtctgttagc
      421 ttgctggaag agtacctgcg ttcatcacct tatgtgatgg accaattaaa agaggcgaaa
      481 atcgatgaac tggatttgca tcgcgctatt gttgcattga gcgaaaaaat gaaagcggtt
      541 gatgacaatg ccagtaagaa aaaagatgaa ccgtcactgt atacctcctg gacgctaagt
      601 tttaccgcgc caaccagtaa agaggcgcag accgttttga gcgggtatat cgattatatc
      661 tctgcgttgg tggtgaaaga gtcgatagaa aacgtccgta ataaactgga gatcaaaacc
      721 cagtttgaaa aagaaaaact ggctcaggat cgcattaaaa cgaaaaatca acttgatgca
      781 aacattcagc gcctcaatta ttcactcgac attgccaacg cggcaggaat taaaaagccc
      841 gtatacagta atggtcaggc cgttaaagat gaccccgatt tctctatttc tctcggcgca
      901 gacggtattg aacgcaaact ggaaattgaa aaagcggtta ctgacgttgc ggaactgaac
      961 ggtgaattac gtaatcggca gtatcttgtc gagcaattaa caaaagcaaa tgtcaacgat
     1021 gtgaatttta cgccgtttaa atatcagtta agcccgtcat tgccagtgaa aaaagacggt
     1081 ccgggtaagg cgattattgt gatcctttcc gcgctgatcg gcgggatggt ggcttgtggt
     1141 agcgtgttat tgcgctatgc gatggcatcc agaaaacagg atgccatgat ggcagaccac
     1201 ttagtttaac tatttgcggt tgaaggtgcc gttcgtccga gcggcaccac aagcggcgtt
     1261 ccggctaccg gatcgtcaat gatcatgcat cgcagaccat aaatgcgctc aatcagttca
     1321 gcagtgacaa tctccttcgg cgctccctga gcaacaattt tcccttcccg caatgcaatc
     1381 aaatggctgg cgtaacgaca ggcctgatta agatcgtgca gcaccgccgc cagggtatag
     1441 cctttctcgc ggttcagttc gcttaacagt tccagtagat caatctgatg actgatatcc
     1501 agccaggtcg tgggttcgtc gagcagcata attgccgttt cctgggctag caccatcgcg
     1561 atccacgctc gctggcgttg tccgccagaa agggtatcca cgctttgatc tgccagatga
     1621 gttattctcg tggcctgcat cgctttcgtt accgcttctt catcctcttt gcgccagcgg
     1681 gtaaacagcg gttgatgcgg ataacgtcca cgcgccacca gctcctgcac ggtgatatcg
     1741 cccggcgtgg tagcattttg cgccaacaga ccaatccggc gtgcaacttc tttactggcg
     1801 taatgttgaa tgtgctcgcc atccagccag acatgcccgt gagcaggcgt catcaggcgg
     1861 cttaaggtac gcagtaacgt ggatttacca cagccatttg gtccgataat tgccgtgaag
     1921 tgaccatcag gaatttctac agtcagattt tccgcaacgg tatatttgcc atatcccagg
     1981 gttaactgtt cgccgcgcaa acgggctact gattcggtca ttttttgcga gactcctgaa
     2041 ttaacaagac gataaggtaa ataccgccga ggctgacggt aacgacgcca accggaagtt
     2101 gatacggcat aaacagttgt tgggcgcaga gatcggccgc cagcagtaac agcgctccgc
     2161 acagcgccgc ctgggtcagc ccccagcgag cggtgccgct aatgcgtcgg gcaatgtgcg
     2221 gcgcgactaa agcaataaag gaaatcggcc cggcaagcgc cgttgccgca gcggtaagca
     2281 ccactgcaac cagcatcatt aacagacgcg aacgctcgac gctgacgccc agcgcgcagg
     2341 cggtatcatc gcccatctcc agcaagcgca ttcgccgcac cagcaaggcg gcggcaatga
     2401 gcatcaatat gatgatgggt gcggaaggcg aagtttttgc ccacgtcagg ccattgagcg
     2461 atccggcatt ccacaaaccc gctgttagcg ccgtttctaa agacgctttc agcaacagcc
     2521 aagtattaaa ggccaccagc atggcgcgaa cgccgatgcc gataataatc aaccgaaagg
     2581 tgtcgatacc gttacgccag gcgagcagcc agaccaacag agaagtgaca atgccgccca
     2641 ccattgctgc cagcgcgata gccgtcaggt cctgaccaaa cagcaccatc gccaccagca
     2701 cgccgctcca cgccccggtg ttaaagccca ttacgtcagg gctgccgagc gggttacgca
     2761 tcagcgactg aaaaatcgcg ccgctgacgc ccagcgccgc gccaatcaac agcgccatca
     2821 gcacgcgtgg taaacgccat tcggtgacca ccatcgtcat actgcgcggc gcatcgccca
     2881 tcaacgcggc gaatacctgc gagatttcca gcgtgacggc accgctgcgt aatccccaga
     2941 tacctgcaac cacacaggcg gaaatcagca gcaaacaggt gatgagtaat cggcgagaga
     3001 cgtaaatcat gcaccacctc gcgttttacg tcgcacgagg aagatcagca ccggtgcacc
     3061 aataaacgca ctgaccacag aaacgcgcag ttcgccgggc acaatcaccc gcccgatgat
     3121 atcggcaaac agcagcaggg caggggtagc aagtagcgtg acgggcagcg accagcgatg
     3181 atcggcacct accagccagc gcgccatgtg cggcatcatc aggccgataa aggcaatcgg
     3241 gccaactacc gccgtcgcac taccacaaag cacggtaatc gccagcagac caatcaactg
     3301 tgtgcgcgcc acgcgactgc ccagcgccgt cgcggtgtca ctgccgaggc tcaaactgtt
     3361 cagcgcgcga ctcagtaata gcgcagttgc tccggcgatc agaaccggga tcagcaccac
     3421 ttttaaggta tgtagattgc gaatatccag cgaaccggct tgccagaaac gcagttgatc
     3481 gtagacgtca ggattaagca gggcgatgcc gctggtcagc ccttccagca ccgccgccag
     3541 cgccacgccc gccagggtta aacgcaccgg acttaactgc ccgccgccct gactgccgat
     3601 aaaggcaaca atcaatgagg ccaccagcgc cccggcgaag gccatcgcca gttgttcctg
     3661 cgcggatgag taaccaaaca gcgccgcacc cagcacaatg gcaaagctgg ctccggcgtt
     3721 cacgccaagc aagccggggt cggcaagtgg atttcgggtg agggtttgca ttaacgcccc
     3781 agcaagccca agcgcaccgc ctgcaagtaa accggcaagg gtgcgcggca gtcgcgcgtc
     3841 gagcacgatg gtgcagtcgg cgctctggca ggtgcctgag aaggcctcca gcactactga
     3901 cgcggggagt gattttgccc caatgagcag gcttaatgcc gtcgcgataa tcagtaataa
     3961 cagcaatccg ggcacggcaa tggcgcgtgt cacggcaaca gaaccagaca tacgaacttc
     4021 catgataatg aaattaatta tcgttatcga tcttatttgg atatgttagc atgtgcagcc
     4081 taagaatagg tatttaaaat atttgatggc aaggcattgt aatgaataaa caatcctggc
     4141 tgcttaacct cagcctgttg aaaacgcacc cggcgtttcg cgcagtattc ctcgctcgtt
     4201 ttatctcaat tgtgtctctg ggtttgctcg gcgtcgcggt gccggtgcag atccagatga
     4261 tgacgcattc tacctggcag gtggggcttt cggtgacgct gaccggcggc gcgatgtttg
     4321 ttggcctgat ggttggcggt gtgctggcgg atcgctatga gcgcaaaaaa gtgattttgc
     4381 tggcgcgcgg cacctgtggc attggcttca ttggactgtg cctgaacgcg ctgctgccgg
     4441 agccgtcatt gctggcaatc tatttacttg gtttatggga tggttttttc gcatcacttg
     4501 gtgttacggc gctactggcg gcaacacctg cactggtagg gcgtgaaaac ttaatgcagg
     4561 ccggggcgat caccatgttg accgtgcgtc tggggtcggt gatttcgccc atgattggcg
     4621 gtttattgct ggcgaccggc ggcgtagcct ggaactacgg gctggcggcg gcgggcacgt
     4681 ttattacctt gctaccgttg ttaagccttc cggcgttgcc accgccaccg cagccgcgcg
     4741 agcatccgtt gaaatcatta ctggcaggat ttcgttttct gctcgccagc ccgctggtag
     4801 gagggattgc gttgctgggt ggtttattga cgatggcgag cgcggtgcgg gtactgtatc
     4861 cggcgctggc tgacaactgg cagatgtcgg cggcacagat tggttttctc tatgcggcga
     4921 tcccgctcgg tgcggctatt ggtgcgttaa ccagcgggaa gctggcacat agtgcgcgac
     4981 cagggttatt gatgctgctc tccacgctgg gatcgttcct cgccattggt ctgtttggcc
     5041 tgatgccgat gtggatttta ggcgtggttt gtctggcgct gttcggctgg ctgagcgcgg
     5101 ttagttcgtt gctgcaatat acaatgctgc aaacgcaaac cccggaagcg atgttagggc
     5161 ggattaacgg tttgtggacg gcgcagaacg tgacgggcga tgccataggc gcggcgctgt
     5221 tgggtggttt gggcgcgatg atgacaccgg ttgcttccgc aagcgcgagc ggttttggtt
     5281 tgttgattat cggcgtgttg ttattgctgg tgctggtgga gttgcgacgt tttcgccaga
     5341 cgccgccgca ggtgacagcg tctgacagtt agctaaaggt gcattttccc ctcaccctaa
     5401 ccctctc
//
LOCUS       DAZOYS010000234         5351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOYS010000234 DAZOYS010000000
VERSION     DAZOYS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.236"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..68
                     /locus_tag="ACHUN9_003807"
     CDS             <1..68
                     /locus_tag="ACHUN9_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697091.1"
                     /translation="ETLLQQPEERFAWLRSKKDRF"
     gene            140..1498
                     /gene="mltD"
                     /locus_tag="ACHUN9_003808"
     CDS             140..1498
                     /gene="mltD"
                     /locus_tag="ACHUN9_003808"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0697092.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1547..2170)
                     /locus_tag="ACHUN9_003809"
     CDS             complement(1547..2170)
                     /locus_tag="ACHUN9_003809"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0697093.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2174..2974)
                     /locus_tag="ACHUN9_003810"
     CDS             complement(2174..2974)
                     /locus_tag="ACHUN9_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0697094.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3215..4129
                     /gene="yafC"
                     /locus_tag="ACHUN9_003811"
     CDS             3215..4129
                     /gene="yafC"
                     /locus_tag="ACHUN9_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0697095.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4126..4929)
                     /gene="dkgB"
                     /locus_tag="ACHUN9_003812"
     CDS             complement(4126..4929)
                     /gene="dkgB"
                     /locus_tag="ACHUN9_003812"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0697096.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5092..5168)
                     /locus_tag="ACHUN9_003813"
     tRNA            complement(5092..5168)
                     /locus_tag="ACHUN9_003813"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5132..5134),aa:Asp,seq:gtc)
BASE COUNT         1407 a         1294 c         1322 g         1328 t
ORIGIN      
        1 aagaaacatt attgcaacaa cctgaagagc gttttgcatg gttaaggtca aagaaagata
       61 ggttctgata aaactttctt gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta
      121 agcaactatt gacacacaca tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt
      181 gggttgccag agtaccggca acgttcaaca gcacgcacag agcctttctg cagctggtca
      241 aggggaagca gcaaagttta caagtcaggc acgatggatg gacgatggga cgtctatcgc
      301 gccagatggt gacttgtggg ctttcattgg cgacgagcta aagatgggaa ttccggaaaa
      361 tgaccggatt cgcgaacaga aacagaaata tttacgcaat aagagctatc tccacgatgt
      421 aactttacgg gcagagccgt atatgtactg gatagcaggg caagttaaaa aacgtaacat
      481 gcctatggaa ctggtactac tacccatagt ggagagcgct tttgatcctc acgcaacgtc
      541 tggcgccaat gccgcaggca tctggcagat cattccgagc acggggcgca attatggttt
      601 gaaacagacc cgcaattatg acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct
      661 gaatatgatg cagcgtctga acaaaatgtt tgacggcgac tggcttctga ccgtagcggc
      721 ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa
      781 atccacggac ttctggtcgt taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat
      841 gctggcattg agtgatattc tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac
      901 cgatgaaagc cgtgctctgg cgcgtgtgca cctgagtagc ccggttgaaa tggcgaaggt
      961 tgcagatatg gcggggattt ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg
     1021 ctccacgctg ggcgcaagtg ggccgcagta cgtgatggtg ccaaagaagc atgcagatca
     1081 actgcgtgaa tctctcgctt caggcgaaat tgctgctgta cagtcgacgc tggttgccga
     1141 caatacgccg cttaacagcc gtgtttacac cgtacgctct ggcgacacgc tttcaagcat
     1201 cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc
     1261 taagctgaag ccaggccaaa gtttgacgat tggtgcaggc agtagcgcac agcggttggc
     1321 aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc
     1381 taaacgccac ggcgtgaaca tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct
     1441 gcaaccaggc gataagctga cgttgtttgt gaaaaacaac aacatgccag attcctgaca
     1501 aaccagataa taaaaaaggc accgattccc ccggtgcctt ttttatttat gccgctttat
     1561 gtgcttctac catgatgata tcactggtga aagagccatc agtctgcaag gaaaaatacg
     1621 ttctcacctc tgtcgatgcg ctctgttggt aaatgcgaat agcgtctact aacgcttctg
     1681 gcgtacgcat tctcacgacc catgaagaaa attccagcgg taacttatct gtaattaaat
     1741 tatcaactat cagattggct tcattgatta acgtcaacca cgcaccgctg gcgtagtttc
     1801 gtacgtgaga ggtatcgcgt aatgcttcta ccgcctgtaa ccagatgtcg cgcactgggt
     1861 gacccggaga cattacatcc atcacaatca gcctaccgcc aggtttcaat atcctattca
     1921 cttctcgcag tgctgcacca acatcatgcc agtgatgggc agaataacgg ctgataacaa
     1981 tatcaaatgc gttatcggca aatggcagac ttgcggcata tccctggcgg gtggtgatat
     2041 ttttcagttg ccgggcttcg gcagcttgtg ccacgacatc cagcatgtgg gcagataagt
     2101 catacgccac caccgcgctc acgttttgcg cagcgccaaa cgttggtctg ccctgcggca
     2161 gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt agcggattgt
     2221 gatcggaagc gcgcgtaacc agtacagaag cttcactgac gttcagacca cggtagaaaa
     2281 caaaatcgag cgggcgacca aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc
     2341 gcagcgacat ttcccgcgca aagcgatata acgcgttcat ccttctacgg ctccaggcat
     2401 tgaaatctcc cgccataatg accgggccgc tgtggtgagc tatctgatca ccaataggaa
     2461 gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt atattaacca
     2521 ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac ttcgccagac
     2581 gcaaaatggg ttctcgttca cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg
     2641 tcattacgcc agaaggatgt tgtggcagca cgaaagcggg tacctgatcg gcggcaagat
     2701 agttagcggt cgcaaactgt accaactctg gcgttgtttg cgcttcctgc aataacacca
     2761 gatgtgcgtc tttgccgtaa ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt
     2821 atatgttcca caccaggatc cgaatacgct cttcggtact taacggttct ccgggtggta
     2881 atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctctccgca ggttgtccgg
     2941 caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc tctattacgt
     3001 caacccttcc gcactcggga gggttctact gttataggga ttttagctca ctctttcaac
     3061 gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaaatt tgctcattat
     3121 tgagaacatt aaatcggatg acatatcacg catcctgatg gtttaatgac aaataaattc
     3181 aaaatatctc atctattttg caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc
     3241 catttttgtt tcggtcgtcg aaagcggcag ctttagccgg gcagcggaac aattagggca
     3301 agcaaactca gcggtaagcc gggcggtgaa aaagctggaa atgaaacttg gcgttagcct
     3361 gcttaatcgg accacgcggc aacttagcct gacggaagaa ggcgagcgtt attttcgtcg
     3421 cgtacagtca attttgcagg agatggcagc ggcagaatca gaaattatgg agacgcgtaa
     3481 tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc actttctgat
     3541 gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttatcgc tagtctcctc
     3601 cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt
     3661 aacggactcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc
     3721 ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa agcaacatgt
     3781 ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagcct gtagtgatgg
     3841 acaattacat gaggtgaagt acggtttgtc atccaatagt ggggaaacac tgaaacagct
     3901 ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc
     3961 tcgtggagaa ttggtggagt taatggcaga taaagtgttg ccagtggaaa tgccattcag
     4021 tgccgtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta tcgatttcct
     4081 tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat cccattcagg
     4141 agccagacct tccgggctaa ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt
     4201 atcttcggca tcaagctgta aattttgtgc cttaagatta ctttccaggt ttttacgttt
     4261 agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa tcacttgtgc
     4321 cggagtcgca ttgtgtttag ctgcaatacg agcaataacc tcatctttca gtgccttacc
     4381 ataagccagc gtcatatagg aagtgatatg aataccgtgc tgtttagccc aggcaaccac
     4441 tttacggttt tgcagataag gagagagttc aatctggttg gtagcgatgt tttcagcgcc
     4501 aacagcagca atcgcttttt ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg
     4561 tgtcagacct tgttttttgg cctccagcag cgcctgcata aactcttcaa cagagacttc
     4621 atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac gcaatttttg
     4681 caggctctct tttagactcg ggatcaattt gtctttgctg agattttcaa tccagatttt
     4741 agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct gacctactgc
     4801 ggcttcgtta tcatagattt gtgcagtatc aattgcgcga taaccaagtt caagcgccgt
     4861 tttcacagat gaaataacaa cgtcgtcttt gagacggaaa gtacctaaac caaatgcagg
     4921 gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc
     4981 agactgttgc ggaaaaagag cataaaatgc agaggatttt tgcgattctg gcaataatag
     5041 atatacagac gaaaaaaaac ctcgagcgtt aactcgaggc ttcttaataa gtggcggaac
     5101 ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac cgactgaact
     5161 accgctccac cgaattcttt tacaaccacc ggttttatga ccggcttact gcttaatttg
     5221 atgcctggca gtttatgaat cacttcgtga ttcacccttc gggccgttgc ttcgcaacgt
     5281 tcaaaagctt tcgcttttgt cctactctct tatcgagtag tgaactgcct caaaggtcag
     5341 tgccattctt c
//
LOCUS       DAZOYS010000235         5350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOYS010000235 DAZOYS010000000
VERSION     DAZOYS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.237"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUN9_003814"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUN9_003814"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0697097.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUN9_003815"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUN9_003815"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0697098.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUN9_003816"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUN9_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0697099.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUN9_003817"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUN9_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0697100.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1381 c         1267 g         1334 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc
//
LOCUS       DAZOYS010000236         5304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOYS010000236 DAZOYS010000000
VERSION     DAZOYS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.238"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            14..85
                     /locus_tag="ACHUN9_003818"
     CDS             14..85
                     /locus_tag="ACHUN9_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697101.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(62..214)
                     /gene="hokA"
                     /locus_tag="ACHUN9_003819"
     CDS             complement(62..214)
                     /gene="hokA"
                     /locus_tag="ACHUN9_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0697102.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(540..2609)
                     /gene="glyS"
                     /locus_tag="ACHUN9_003820"
     CDS             complement(540..2609)
                     /gene="glyS"
                     /locus_tag="ACHUN9_003820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0697103.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2619..3530)
                     /gene="glyQ"
                     /locus_tag="ACHUN9_003821"
     CDS             complement(2619..3530)
                     /gene="glyQ"
                     /locus_tag="ACHUN9_003821"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0697104.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3625..3924)
                     /locus_tag="ACHUN9_003822"
     CDS             complement(3625..3924)
                     /locus_tag="ACHUN9_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0697105.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4099..5094
                     /gene="wecH"
                     /locus_tag="ACHUN9_003823"
     CDS             4099..5094
                     /gene="wecH"
                     /locus_tag="ACHUN9_003823"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0697106.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5136..>5304)
                     /locus_tag="ACHUN9_003824"
     CDS             complement(5136..>5304)
                     /locus_tag="ACHUN9_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0697107.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         1250 a         1369 c         1312 g         1373 t
ORIGIN      
        1 gggtgcaatg agaatgcgca acgccgtaag aaaggcggga ataacttccc gccaaagact
       61 cttactcttt caaattgcag gctaaaaatg ccgccagctc ataactctcc tgtttaatat
      121 gcaattcaca cagtgaatct cttatcatcc aggtgaaaaa taaaagcgtg aaacaaatca
      181 ctattaaaga aagtaatcta tatttctgcg gcattcccag cctcctgtgt tgatttccaa
      241 cgagtaagag gctaacctta tggtgttcag gcatagggta gcctcacttt gatttatagt
      301 caggtggggc ttttctctgt ctgcctttcg gtgaatacct gagacaaaca gtctcaagca
      361 cccgtggcta ttctagctta ataagtttgt ttcttctcct tgatataatc ctaaaaaaac
      421 ctcataaaat taatatatga gataatcttt attcaacaga agattattaa aggttgctgt
      481 attatttagc gataataaaa aaagcctgcc agatggcagg ctttttaata acggcgttat
      541 tactgcaaca gcgaaatatc cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg
      601 gtcagacggt tgagacgcaa ttctttgtca tcaaccatga ccatcacttt atcgaagaaa
      661 gcatcaaccg gctcacgcag ctctgccagt tcgaccagcg catcctggta acgaccttcc
      721 gcaaagtacg gctccagctt gtcacgtagc accacaacct gcatcgccag tttaatttct
      781 tccggctctt tcagggtaga ggcattcacg cggtcgctca gcacttcgtc agatttcgcc
      841 agaatgttag atacacgttt gttcgccgcc gccagtgcag cagctgcatc cagggtacgg
      901 aaatgcgata ccgctttcat acgggcatcg aaatcagccg gacgagtcgg acgacgcgcc
      961 agtaccgcct ggatggtgtc aacggtgtaa ccttcgtcct gataccaggc gcggaagcga
     1021 ccgagcataa agtcgataac atcatcaact acgttggcgt tagtcagctt atcgccatac
     1081 agacgcactg cttcttcggt cagcgtttgc agatcaaggt tgaggttctt ctcaacgata
     1141 attcgcagca cgccaagtgc ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga
     1201 tgctgaccga taccgaagat acccgccaga gtatccatct tatcagcaat cgccagcgca
     1261 caagctaccg ggttggacgg caggtcatca ccggcaaagc gcggctgata ctgctcattc
     1321 agcgcgacgg ctacgtcttc cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg
     1381 ccttgggtgt cggtgaactc gaagaccatg ttggtcatca ggtcacactt agacagcagg
     1441 cccgcacggg ttgcgtggtt aacgtcagcg ccaatctgtt cagcaatcca gccagccagc
     1501 gcctggatgc ggtcagtttt gtcacgcagc gtacccagct gttgctggaa cagcacggtt
     1561 tgcaggcgcg gcaggttatc ttccagacgt tttttacggt cggtattgaa gaagaactcg
     1621 gcatccgcca gacgtggacg aacgactttc tcgttaccag agataatttg ctgcggatct
     1681 ttcgattcga tgttggcaac gaagatgaag ttcggcagca gtttgccatc gttcgcatac
     1741 accgggaagt atttctggtc acctttcatg gtgtaaacca gggcttcagc cggcaccgcg
     1801 aggaatttct cttcgaattt tgcggtcaga acgaccggcc actccaccag cgaagccact
     1861 tcttccagca ggctttcgct taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg
     1921 gcatcggctt taatcttcgc tttacgttct tcgtaatcgg cgatgacttt cccgcgctca
     1981 cgcagaattt ccggatactg atcggcgttg tcgatggtga attccggttc gcccataaag
     2041 cggtggccgc gaatcacgcg atcggactga atgcccagaa tggttgccgg aatgactttg
     2101 tcgcccagca gcagggtcac ggtgtgcacc ggacgcacga agtgcacgtc gcttgcgccc
     2161 caacgcatca gtttcgggat cggcagtttc gccagcgaag tcgcaaccat attcggcagt
     2221 agtgcttcag tgctttcgcc cttcacatgg gcgcgataca gcagccattc gcctttatcg
     2281 gtagtcagac gctcagcctg gtcaacggta ataccgcaac cacgcgccca accttctgcc
     2341 gctttgctcg gtttgccttc agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg
     2401 atttcacgat ctggttgcgc ttccgccagg ttagctactt tcaacgccag acgacgcgga
     2461 gcagcaaacc attgaacggt gccgtgtgcg aggccagcgt tatccagctc cgcagtaaag
     2521 ttcgcagcaa aggactcagc caggctgcgc agtgcttttg gtggcagctc ttcagtgccg
     2581 atttccacca gaaaagtttt ctcagacata gccgcctctt acttatcttt gttgcacatc
     2641 gggaagccga gggcttcacg ggaagcgtag tatgcttctg ccactgcttt ggtcagggtg
     2701 cgaatgcgca gaatgtagcg ctgacgctca gtgacggaga tggctttacg cgcatccagc
     2761 aggttgaagc tgtgggcggc tttcagaata cgctcgtagg ctggcagcgg cagcggattt
     2821 tccagcgcca gcagctgctg cgcttctttc tcgtactgct cgaagcaggt gaacaggaaa
     2881 tccacatccg cgtattcgaa gttgtaagtg gactgctcca cttcgttctg atggaacacg
     2941 tcgccgtagg tggttttacc cagcgggccg tcgctccaga ccaggtcgta aacgctgtct
     3001 acgccctgaa tgtacatggc cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt
     3061 ttacactcca gaccaccaac ctgctggaag taagtgaact gcgtcacttc catgccgttt
     3121 agccacactt cccagcccag tccccaggca cccagagtcg ggttttccca gttatcttcc
     3181 acgaaacgga tgtcgtgaat agtcgggtcc atgcccagct ctttcagaga accgaggtac
     3241 agctcctgaa tattgtccgg cgatggctta atgaccacct ggaactgata gtagtgctgt
     3301 aaacggttgg ggttttcgcc gtagcgacca tcggtcgggc gacgagaagg ctgaacataa
     3361 gcagccgcca tcggttctgg ccccagcgcg cgcagacagg tcattgggtg agaggttccc
     3421 gcgccgactt ccatgtccaa tggttgaaca atggtgcagc cctggcgagc ccagtaatcc
     3481 tgtaaggtca ggatcaagcc ctggaaggtc ctggtatcaa acttttgcat attatttcgt
     3541 gctggatacg tgtggattta aaggaaggga tcagtatacc cgctggatgg aagatataca
     3601 gtacgaaacg ggaaaaagca gggcttaacg catggaaaga tgcaaaaaat ggccatctgc
     3661 gtcaaaagaa cagacaaact gctctttacg gaaggtataa ccgcgcattt catagcttcc
     3721 ctggaactgt tcgaaggcgg tgacgtcgat tttttgctcg ccggtgttat aacgttttgc
     3781 cgcctgatcc ttgcataacg cttccatatt caacgaacgc agaggatcaa cctttacccg
     3841 ttgtgctttc tgcacgggtg acggtgtaga ccaacccact agcaccataa gcgccattgc
     3901 cggaaagaaa gcgttcatca tcattaaatt accgtctggt ttgcaggtga gtcttattat
     3961 ttatatcggt atagtggctg atatcttacg aatttcctta agcctctata agctgtagtg
     4021 ataaaactca gattattcca gtgcgccaac caaatgattt atcggtcaga ctttttttaa
     4081 ccagatacag agacacagat gcagcccaaa atttactgga ttgataacct gcgagggata
     4141 gcgtgtttaa tggtggtgat gattcacacc actacctggt atgtgaccaa tgctcatagt
     4201 gttagccccg tcacatggga tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg
     4261 ccgctatttt tcatgatttc cggctatctc ttttttggcg aacgcagcgc ccagccgcgc
     4321 catttcttgc gtatcggctt atgtctgatt ttttatagcg caatcgcact gctctacatt
     4381 gcgctgttta cctccatcaa tatggagtta gcgctgaaaa acctgctgca aaagccagtg
     4441 ttttaccact tgtggttttt cttcgcgatt gcggtgattt atctggtttc accgctgatt
     4501 caggtgaaga acgtcggcgg aaaaatgttg ctggtgctaa tggcggtgat tggcattatc
     4561 gctaacccaa acacagtgcc gcagaaaatt gacggttttg aatggctgcc aattaactta
     4621 tatatcaatg gcgatacttt ttactacatt ctgtatggca tgttgggccg cgctataggg
     4681 atgatggaca cacagcataa agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg
     4741 gtttttatta tctctcgcgg gacattatat gaattgcagt ggcgcggaaa ttttgccgat
     4801 acctggtatc tttactgtgg gccgatggtt tttatctgcg caatcgcgct attgactctg
     4861 gttaaaaaca cgctggatac gcgtaccatt cgcggacttg gcttaatctc ccgccattca
     4921 ttgggtatat acggattcca cgccttgatt atccatgcgc tgcgcacccg gggaattgag
     4981 cttaaaaatt ggccaatact ggatattatt tggatctttt gcgcgacgtt ggcagcgagt
     5041 ttgttacttt ctatgctggt acaacgaatc gacagaaaca gattagtgag ttaagtaaaa
     5101 gcccgttcac attggactga ccgggcttac gtgaattatt cgctgtattc ttcctgggta
     5161 acctgtgttt cttttggcgg gtttattccg gcatcacgaa tattcttctg caccgctact
     5221 gcaccaaaca agcttaagaa gaaagccagt ccataaaaac ctttttcact gagtaataat
     5281 gtcgcattcc acagacctac catc
//
LOCUS       DAZOYS010000237         5302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOYS010000237 DAZOYS010000000
VERSION     DAZOYS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.239"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            325..411
                     /gene="leuL"
                     /locus_tag="ACHUN9_003825"
     CDS             325..411
                     /gene="leuL"
                     /locus_tag="ACHUN9_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0697108.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            504..2075
                     /gene="leuA"
                     /locus_tag="ACHUN9_003826"
     CDS             504..2075
                     /gene="leuA"
                     /locus_tag="ACHUN9_003826"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0697109.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2075..3166
                     /gene="leuB"
                     /locus_tag="ACHUN9_003827"
     CDS             2075..3166
                     /gene="leuB"
                     /locus_tag="ACHUN9_003827"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0697110.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3169..4569
                     /gene="leuC"
                     /locus_tag="ACHUN9_003828"
     CDS             3169..4569
                     /gene="leuC"
                     /locus_tag="ACHUN9_003828"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0697111.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4580..5185
                     /gene="leuD"
                     /locus_tag="ACHUN9_003829"
     CDS             4580..5185
                     /gene="leuD"
                     /locus_tag="ACHUN9_003829"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0697112.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5234..>5302)
                     /locus_tag="ACHUN9_003830"
     CDS             complement(5234..>5302)
                     /locus_tag="ACHUN9_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697113.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1352 a         1405 c         1398 g         1147 t
ORIGIN      
        1 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
       61 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
      121 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
      181 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
      241 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
      301 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
      361 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
      421 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
      481 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
      541 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
      601 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
      661 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
      721 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
      781 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
      841 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
      901 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
      961 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     1021 gctacaccat gccgcttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     1081 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     1141 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     1201 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     1261 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     1321 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     1381 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     1441 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     1501 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     1561 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     1621 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     1681 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     1741 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     1801 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     1861 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     1921 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     1981 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     2041 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     2101 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     2161 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     2221 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     2281 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     2341 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     2401 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     2461 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     2521 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     2581 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     2641 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     2701 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     2761 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     2821 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     2881 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     2941 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     3001 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     3061 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     3121 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     3181 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     3241 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg
     3301 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     3361 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     3421 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     3481 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     3541 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     3601 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     3661 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     3721 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     3781 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     3841 gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     3901 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     3961 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     4021 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     4081 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     4141 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     4201 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     4261 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     4321 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     4381 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     4441 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     4501 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     4561 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     4621 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     4681 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     4741 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     4801 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     4861 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     4921 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
     4981 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
     5041 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
     5101 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
     5161 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
     5221 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
     5281 taaccgcaat tacccagtag ac
//
LOCUS       DAZOYS010000238         5292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOYS010000238 DAZOYS010000000
VERSION     DAZOYS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.240"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(43..444)
                     /gene="crl"
                     /locus_tag="ACHUN9_003831"
     CDS             complement(43..444)
                     /gene="crl"
                     /locus_tag="ACHUN9_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0697114.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(502..1746)
                     /gene="frsA"
                     /locus_tag="ACHUN9_003832"
     CDS             complement(502..1746)
                     /gene="frsA"
                     /locus_tag="ACHUN9_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0697115.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1838..2296)
                     /gene="gpt"
                     /locus_tag="ACHUN9_003833"
     CDS             complement(1838..2296)
                     /gene="gpt"
                     /locus_tag="ACHUN9_003833"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0697116.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2557..4014
                     /gene="pepD"
                     /locus_tag="ACHUN9_003834"
     CDS             2557..4014
                     /gene="pepD"
                     /locus_tag="ACHUN9_003834"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0697117.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4071..4685)
                     /gene="prfH"
                     /locus_tag="ACHUN9_003835"
     CDS             complement(4071..4685)
                     /gene="prfH"
                     /locus_tag="ACHUN9_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0697118.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4682..5292)
                     /locus_tag="ACHUN9_003836"
                     /pseudo
     CDS             complement(4682..5292)
                     /locus_tag="ACHUN9_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1300 a         1420 c         1299 g         1273 t
ORIGIN      
        1 ttaatggata ttgccggatg tgatacatcc ggcacatttc actcacgccg ttaacttcac
       61 cggttcatca cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg tcagcagttc
      121 acgcagcttc tcgtgaaact cacgtagggt gtgctccagt cgttcaacca cttcagtgtc
      181 ttttaccgga acactcttcc agtcgcctgc tttatcgaac agaccaaact ggtaactgta
      241 ggtaaaacgg gattcctgcg cttcaagctc catccaccag ccccagaact cacgcacttc
      301 cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc gattatcttc
      361 gcacttacct tcacgaatat acgggcctag tgcggtaaat tttttgatca atctgctctt
      421 cgggtgtcca ctcggtaacg tcattgcaat ctcctgttgt gatgcaactg ttttaccaaa
      481 ttggcaaatt ttagcaaatt tttaacacaa gcgtttttcg atccagccgg tgatttcctg
      541 aagacctttg tcaaaattcc gatacaccgg gttaaatggg atctctaata atttaccgtc
      601 agcagatgat gaggtgatta agcgtgagtc ctcttccggg ctgaacggat cgttcttcca
      661 gtagcctgat aacattggcg ttgggcagcg acgccccagc aatccttgca cttttaatga
      721 atagcgattc agctccacgc gtaacgcttc gtcggaagca tcatgcatcc ccaaacgact
      781 cgccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat cactcaacag
      841 ggtatgaact accggaccaa gacaggcaac cgctttcaga cgcggcgatt caagatatgc
      901 cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc
      961 cacccacggt acgttaggca gagcctttaa aacgtgctga tgcaacaggc tggagtcctg
     1021 ggtgagcttc cattttgatg aaaagcccac cgacggcata tcaatagtca gcatcgcaat
     1081 gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca tcgcatccag
     1141 accaccacac attaatactg tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc
     1201 ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc ccggcagacg
     1261 ctgggcggct tcttcatagg cacggtttga caaagcctgc gcttgctcgg ccaggtcatc
     1321 tcctttcaga tgaggatagg cagcaatgtt gtacaacgta gccgcatgca gccagtgacg
     1381 accactgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca tcgcctggat
     1441 ggcccactca taaatccagt tgccaccacg gtagccaatc accgtatcgt ataaatcatc
     1501 gtcggtacgt tcggcatcgc tcatcacaat acgcgcctgg acgtcgagga tttcacgtgg
     1561 gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt
     1621 tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag
     1681 cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg
     1741 tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa
     1801 aaagaacaac gcccggcagt gccaagcgtt gaaaagatta gcgaccggag attggcggga
     1861 cgaatacgac gcccatatcc cacggctgtt caatccaggt atcttgcggg atatcaacaa
     1921 catagtcatc aaccagcgga cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt
     1981 ttggatacat ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa
     2041 cgatgaagcc ttcgccatcg ccttctgcgc gttttagcac tttaagctcg cgctggttgt
     2101 cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca
     2161 gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc cattgttcag
     2221 aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga
     2281 tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg
     2341 aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac
     2401 atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca
     2461 aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct gtgacctgca
     2521 atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc tcaattatct
     2581 ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc gtcctatcat
     2641 gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa
     2701 cgcgatcagg taggtaatat cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt
     2761 aaatcggtcg tcttacaggc ccacctcgat atggtgccgc agaaaaataa cgacaccgtg
     2821 catgacttca cgaaagatcc tatccagcct tatattgatg gcgaatgggt taaagcgcgc
     2881 ggcaccacgc tgggtgcaga taacggcatt ggtatggcgt ctgcgctggc ggttctggct
     2941 gacgaaaacg tggttcacgg cccgctggaa gtgctgctga ccatgaccga agaagccggt
     3001 atggacggtg cgttcggctt acagagcaac tggttgcagg ctgatattct gattaacacc
     3061 gactccgaag aagaaggtga aatctacatg ggttgtgcgg ggggtatcga cttcacctcc
     3121 aacctgcatt tagatcgtga agcggttcca gctggttttg aaaccttcaa gttaacctta
     3181 aaaggtctga aagggggtca ctccggcggg gaaatccacg ttggcctggg taatgccaac
     3241 aaactgctgg tgcgcttcct agcgggtcat gcagaagaac tggatctgcg ccttatcgat
     3301 ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac cattgctgtc
     3361 gcagctgata aagtcgacgt cctgaaatct ctggtgaata cctatcagga gatcctgaaa
     3421 aacgagctgg cagagaaaga gaaaaatctg gccttgttgc tggactctgt agcgaacgat
     3481 aaagctgccc tgattgcgaa atctcgcgat acctttattc gtctgctgaa cgccaccccg
     3541 aacggtgtga tccgcaactc agacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc
     3601 ggtgtggtga ccatgactga taataacgta gaaattcact gtctgatccg ttctctgatc
     3661 gacagcggta aagactacgt ggtgagcatg ctggattcgc tgggtaaact ggctggcgcg
     3721 aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg acgctaattc tccggtgatg
     3781 catctggtac gtgaaaccta tcagcgtctg ttcaacaaaa cgccgaacat ccagattatc
     3841 cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga catggtttct
     3901 atcgggccaa ctatcaccgg tccacactct ccggatgagc aagttcacat cgaaagcgta
     3961 ggtcattact ggacactgct gactgaactg ctgaaagaaa ttccggcgaa gtaattattt
     4021 gatttgctgc cggatggcgt ttaatcgcct tccggcagtt tcatccttca ttatccttcg
     4081 ataaaagcca tccctgtaaa tgtccgtctc gggttgccac gttcaatctg gtgatggaac
     4141 attcgccgct gcgatttcag caccgcgcta ttttcctgtt gctgctgctc cagcttccag
     4201 gcaatcagca atcgtgccag ccgcttgtta gcatgctgac tacgctctga ctgaaccttc
     4261 acgctaatac cggatgccaa atgcgtggcg cgtaccgccg agtcggtttt attgacatgt
     4321 tgaccgcccg gccccgacga acgcaacgtc tcataacgga ttgcatccga ttgttcctgc
     4381 tcatcagcgg taaaacgccc aatgcccaga aaccagtttt tgcgcccatg atgaggccga
     4441 tacggactcg gacaaatcca ctgaatagtg ccgcaccagc tttcgcttaa tgcccatgcg
     4501 ttatcgccat ccagagaaac cagagctgaa cgcagcgtgt cagagtagcg gcccgtttct
     4561 gtttccaaca ccgttaccgc gacgtcttgt cgggcagctt ctttaattaa tcgctccagc
     4621 gcctttttca ctgcgagaca acattcttcc ggcccctgcg ctgaggagag ttgtagcaag
     4681 atcatccttt tttccctcca ctgtttttga gcgttagcac cggacgcagc cgcgcgacag
     4741 gaataattaa accagcctgc accagacatt gcaccacgct ttcagccgat ttataggctt
     4801 gtggcgcttc ttcaaagatg agttgtttat cgcgacaaat tacccgactg ccaagttcag
     4861 tacgtgagag ctgcgtcgct gtgtatttcg ctgccagacg ccctttacac tcggtgcgcc
     4921 cccatttacg cccagcccca tgcgccagcg agtggagcgt tttttcgttc gcaacaggct
     4981 gtacaagcta ggagtaatca ccgagtgaac cgggaataat caccagaccg ttgtcatccg
     5041 gcgtggcacc tttacgatgc aaccagccct gttgatcgcc gatttgacac gcgctaacaa
     5101 agttatgcgc cacatccaga accggacttc cggtggcctt aacctgttgc attatgcgta
     5161 aagagatcag ctggcgatta atacgcgcaa acgccagcgc atcgtcatgt tccgcaatat
     5221 accgtagcgc gtcgtcactg ccttcaggca aaccatgatg cgaaaacgag gcaatatgcc
     5281 gctgtaaaat ag
//
LOCUS       DAZOYS010000239         5269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOYS010000239 DAZOYS010000000
VERSION     DAZOYS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.241"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            266..2971
                     /gene="malT"
                     /locus_tag="ACHUN9_003837"
     CDS             266..2971
                     /gene="malT"
                     /locus_tag="ACHUN9_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0697119.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3014..4030)
                     /gene="rtcA"
                     /locus_tag="ACHUN9_003838"
     CDS             complement(3014..4030)
                     /gene="rtcA"
                     /locus_tag="ACHUN9_003838"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0697120.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4034..5251)
                     /gene="rtcB"
                     /locus_tag="ACHUN9_003839"
     CDS             complement(4034..5251)
                     /gene="rtcB"
                     /locus_tag="ACHUN9_003839"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0697121.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
BASE COUNT         1248 a         1487 c         1323 g         1211 t
ORIGIN      
        1 tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat ctgatgaaca
       61 taagggaaac cagtattcac gctggatcag cgtcgtttta ggtgagttgt taataaagat
      121 ttggaattgt gacacagtgc aaattcagac acataaaaaa acgtcatcgc ttgcattaga
      181 aaggtttctg gccgacctta taaccattaa ttacgaagcg caaaaaaata atatttcctc
      241 attttccaca gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg
      301 actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt
      361 ccggctggcg ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg
      421 ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga
      481 gcgtttcgcc agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat
      541 atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct
      601 tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct
      661 gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa
      721 tctcaccctg gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt
      781 tcgtgaccaa ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa
      841 gcagtttttt gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gttggatttg
      901 cgatgacgtt tccggttggg cgacggcact acagctaatc gccctctccg cccggcagaa
      961 tacccactca gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc
     1021 ggattatctg gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt
     1081 gaaaagcgcc attttgcgct caatgaacga tgccctcatc acccgtgtga ctggcgaaga
     1141 aaacgggcaa atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga
     1201 tgataccggc gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg
     1261 ccagtgggaa ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat
     1321 ggcccaggga tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat
     1381 gctgcgcgat attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct
     1441 gctggaagag tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt
     1501 gttattgcag gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct
     1561 agcccgtgct gaacatgaaa tcaaggacat cagagaaggc accatgcacg cagaatttaa
     1621 cgctctgcgc gcccaggtgg cgattaacga tggtaatccg gatgaagcgg aacggctggc
     1681 aaaactggca ctggaagagc tgccaccggg ctggttctat agccgcattg tagcaacctc
     1741 ggtgctgggt gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctaatgca
     1801 gcaaaccgaa cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat
     1861 ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacacagga
     1921 aaaaacgttc cagctgatca acgagcagca tctggaacag ctgccgatgc atgagtttct
     1981 ggtgcgcatc cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc
     2041 ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct
     2101 ggcaatgttg attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagct
     2161 gaaccgtctg gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc
     2221 caacaaagtc cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg
     2281 gttgcgtcat acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg
     2341 caacattgcc cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga
     2401 agaactcaat gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt
     2461 gctgcttaat caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct
     2521 ggacgcatta aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga
     2581 agcgatggcg caacaactgc gccagctgat tcaactcaat acgctgccgg aactggaaca
     2641 gcatcgcgcg cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca
     2701 tttcgatgag aatttcgttg aacgtctgct gaatcatccg gaagtgccag aactgatccg
     2761 caccagcccg ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag
     2821 caatgagcaa attgcgggcg agctggaagt ggcggcaacc accatcaaga cgcatatccg
     2881 caatctgtac caaaaactcg gcgtcgccca ccgtcaggcc gcggtacaac acgcgcagaa
     2941 actgctgaag atgatgggat atggggtgtg agtttagccg gataatgcat tggatccggt
     3001 ttacatcttt acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa
     3061 accgcaccgg caagaaacgc tccaccacgg cgatattggt cagcagatgg catgaaggat
     3121 gggcgaccgt aaattctccc gcgcccgcca gcgccatcgg tagcaccagc tggtcggcga
     3181 gatattcccc cactaccgcc gggcttgcca ggtagcgttt cacctctttc actaactgcg
     3241 cggctaccac ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat
     3301 tttcactttc gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat
     3361 gaatattctg ttcatgcagg gaaaaactcc ccgccagtgt agcgatttca cgctcagcaa
     3421 catggcgcgg cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct
     3481 cgccaagttg caaggtgtta aacgatgcca cgggcgagac ttccgttgcc accacgccgc
     3541 ctccggcagg ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg
     3601 ccagcagcgg ctccagcacc cggcggataa aatccgcagg cggggccgac gggttatcgg
     3661 tgcctccgct cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg
     3721 tttgcagcac cagcgtacag cttccggcac taccgatagc aaagcggtac tcgccgccgc
     3781 gcacggtgcc aagccggaag accagacgct gcgaccccag ctccgcgcct tccaccgttg
     3841 ccctacaaat ttccgcagcc gcttttaccg cggtcagatg ctggcgcaac agccccggtt
     3901 tcgcccgccc ggcacgaatg ctggtgatgg taaatggctg gccggttatc atcgacaggc
     3961 tcagcgccga gcgcaggatc tgcccgccac cttcgccctg tgcgccatcc agcgcaatca
     4021 tccttttcat catttatcct tttacgcaca ccacctgacg cagggtatag ataacttcca
     4081 ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt
     4141 cgatcacttc ggcatcttta cggcattcca catgcgcggt ggcacgaatt tgatcttcca
     4201 cgctgaacag ttttttcgct ttagtacggc tcattacccg cccggcaccg tggctgcacg
     4261 aacagaaaga ttcttcattt cccagcccac ggacgataaa gctttttgct cccatcgacc
     4321 ctggaataat tccatattga ccagcacgcg caaacaccgc gcctttacgc gtcacgtaga
     4381 tctcttcacc aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca
     4441 tcgccagcgt ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaat gccgttacca
     4501 cgttttccat catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc
     4561 ttaggtaatc atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca
     4621 acgtctcaag ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg
     4681 cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa
     4741 ggcagatttc aataaagtgg ttacccgttc ccaggtgttt atagttgtta gtgttaagga
     4801 aacgcggata tttttgcgtt aaccactgat aaccggcctc cagctcagcc catttcgcat
     4861 cgacgttaaa aggcgggttt tcccatgcgc ctttatcacg tttacaacgc ccggtagtac
     4921 gcccgtgtgg tacggccgtt tcaatcgctt gacgcagctc cgccaggttt tcaggcaggt
     4981 ctgccgccgt taacgcggta cgcagcgcgt tcattccaca gccaatatcc acgcccaccg
     5041 ccgccggaat aatcgcccct ttggtcggga tcacgctacc aatggtggaa cctttaccca
     5101 ggtgtacatc aggcattacc gcaatatgtt tgaaaataaa cggcatcttc gccgtattaa
     5161 taagttgctg acgcgcatcg gcttctaccg gcacgccttt ggtccacatt tttaccgggg
     5221 cattttcagt ggtcagtaat tcgtaattca ttttgttttt ctcttttcg
//
LOCUS       DAZOYS010000240         5249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOYS010000240 DAZOYS010000000
VERSION     DAZOYS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.242"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHUN9_003840"
     CDS             <1..365
                     /locus_tag="ACHUN9_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR0697122.1"
                     /translation="ITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERL
                     GERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFL
                     LRKDIMRRLTQDVCEEIE"
     gene            complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUN9_003841"
     CDS             complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUN9_003841"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0697123.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUN9_003842"
     CDS             3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUN9_003842"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0697124.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUN9_003843"
     CDS             4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUN9_003843"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0697125.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
BASE COUNT         1291 a         1400 c         1286 g         1272 t
ORIGIN      
        1 aaattaccgg acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg
       61 aagtagaaga tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc
      121 ccgatgaacg tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt
      181 cattatcgct ggaagaggtg gtggcttttt ttagccgtaa acgggtcgca aaatataaat
      241 atcctgaaca tatcgtggta atcgaaaaac taccgcgtac tgcctcaggt aaaatacaaa
      301 agtttttgtt aagaaaagat attatgcggc ggttaactca ggatgtctgt gaagagattg
      361 aataagtttc atcttcgggg atcacataac cccggcgact gaacgccgcc ggggatttat
      421 tttatttctt cagttcagcc aagcttaacc aggtttgcac cacggtgtcc gggttcagag
      481 acaggctatc gatcccctct tccatcaacc atgcggcaaa gtcttcgtgg tcggacggac
      541 cctgaccgca aatcccgaca tatttgccct gtttcttcgc ggcacggata gccatcgaca
      601 gcagtgcttt caccgcatcg ttgcgctcat cgaacaattc agacaccacg ccggagtcac
      661 ggtccagacc gagcgccagc tgcgtcatat cgtttgagcc aattgagaag ccgtcgaaat
      721 attcgaggaa ctgctcggcc agcaaggcgt tggacgggat ttcacacatc atgatgattt
      781 tcagcccgtt ctcaccacgc ttcagcccct gacgcgccag ttcctcaacc accgctttcg
      841 cctgatctac ggtacgcacg aacgggatca tgatctcaac gttggtcagc cccatgtcgt
      901 tgcgcacacg tttcactgct tcgcactcca gcgcgaagca gtcgcggaag ctgtcggaaa
      961 catagcgtcc cgcgccacgg aagccgagca tcgggttctc ttcatctggc tcgtaacgct
     1021 caccaccgac caggttggca tattcgttcg atttaaaatc agagagacgg acaatgacgc
     1081 gcttcggata aaacgcggca cccagcgtcg cgatcccttc agtcagacga ccaacgtaaa
     1141 attcacgcgg agaatcaaaa cctttcatca tctcgcggat ttcattttgc aactgcggtt
     1201 cctgatcgtc aaactcaagc agtgcgcgtg ggtggacgcc aatcatacgg ttgatgataa
     1261 attccagacg cgcaaggccc acgccttcgt tcggcaggca ggcgaagtcg aaagcacggt
     1321 ccgggttacc gacgttcatc atcactttca acggcagatc cggcatcatt tctacgctgg
     1381 agcttttcac gctaaattcc agcaactccg catagacgta accggtatca ccttcggcac
     1441 aagaaacagt gacgttctca ccgtctttca tccgttctgt tgcatcacca cagcccacta
     1501 ccgccggaat gcccagttca cgagcgatga tcgccgcgtg acaggtacga ccgccacggt
     1561 tggtgacgat ggcagatgct ttcttcatga tcggttccca gtccgggtcg gtcatgtcag
     1621 tgaccagcac gtcaccaggt tcgatgcggt tcatttcgct gatctcatgg atgactttca
     1681 ccggacccgc gccgatgcga tgaccgatag cacggccttc ggcgataatc ttaccctgtg
     1741 aatgcagcgt ataacgctcc atgacctgac cgcgtgagcg cacggtttcc ggacgcgcct
     1801 gcacaatgaa cagtttgcca gtgtggccat ctttcgccca ctcaatatcc atcgggcgac
     1861 cgtagtgttt ctcaatttgt acggcctgtt ttgccagttc ctgcacttct tcgttggtca
     1921 gcgagaagat gtcacgctgt tcctgcggta cgtcttcgat tttaacctgc ttgccgtgct
     1981 cctgggtcgg cgcgtaaacc atgcggattt ttttcgaccc catggtgcga cgcacgatag
     2041 ccgggcgatt cgccgccagt gtcggtttat ggacgtaaaa ctcatccggg ttaaccgcac
     2101 cctgcacgac catttcccca aggccccatg cggaagtgat aaacaccacc tggtcaaagc
     2161 cggattcggt atcaatggag aacatcacgc cagaagatgc gaggtcggag cgcaccatcc
     2221 gctgaacacc ggcagagagc gccacgccac ggtgatcgta accctggtgc acacgataag
     2281 agatggcgcg atcgttaaac agagaagcaa atacatgctt cactgccacg agaacggcgt
     2341 caaaaccctg aacgttgagg aaggtttcct gctgaccggc aaaagaagcg tccggcatat
     2401 cttctgcggt ggcggaggag cgcaccgcaa aagaggcgtt ttcgtcatcg gcggaaagct
     2461 gtgcataggc ttcgcggatg gcgttttcca gctcaggctg gaagggagtg tcgataatcc
     2521 actggcggat ttgcgcgccc gctttcgcaa gctgagtaac atcgtcaata tccgttttat
     2581 ccagcagttc ataaatgcgc tggtttacgc cgctttggtc cagaaactgg ttaaacgcgt
     2641 cggcggttgt agcgaaacca ttcggaacgg aaacacccat tccggaaagg ttagtaatca
     2701 tttcacccag ggaggcattt ttgcccccaa ccctgtctac atcattcatg ccgagttggt
     2761 tataccaaag caccagcggt gacgagccat tgttggacat cgaacaatcc ttttgtgata
     2821 aatgaacggt ttgagaaaca catttctgcg catttatctt tgcatattta accggatgaa
     2881 aaaaacggtg aatcgttcaa gcaaatatat ttttttactt tttaagactg atcccagcgt
     2941 tgcgcaaatc tgctctcttc gacaatttcc acataaacca tcggtataaa cgaaaccata
     3001 aaaatgaaat gctgttttca taaaaaataa aattgaaggt tcattttata aaccagacat
     3061 aacgtcctac gcttctgtgc gtttttaatt tatgctttca tagaattatg tctgcatcac
     3121 gggaagaaca aaatggataa tgctgttgat cgccacgttt tttatatttc tgatggtacg
     3181 gcaataactg cggaggtatt aggacacgca gtaatgtcac aatttcccgt cactatcagc
     3241 agcattacgc tgccgtttgt cgaaaatgag agccgtgcac gggcagtgaa ggatcagatt
     3301 gacgcaattt atcaccagac aggtgtgcgc ccgctggtct tctactccat cgtgttgccg
     3361 gagattcgcg ccatcatctt gcaaagtgaa ggcttttgcc aggatatcgt tcaggcgctg
     3421 gttgccccgc tacaacaaga gatgaaactg gatccaacgc cgattgctca tcgtacccat
     3481 ggccttaacc ctaataatct caataaatat gatgcgcgca ttgcggcgat tgattacacc
     3541 ctcgcccacg atgacggcat ttcgttgcgc aatctggatc aggctcaggt gatcctgctc
     3601 ggtgtttctc gctgtggtaa aacccccacc agtctttatc tggcaatgca atttggtatc
     3661 cgcgcggcaa actacccctt tattgccgac gatatggata atctggtgct acccgcgtcg
     3721 ctcaaaccgc ttcagcataa attgttcggc ctgactatcg acccggaacg tctggcggcg
     3781 attcgcgagg aacgtcggga gaacagtcgc tatgcctcgc ttcgtcagtg caggatggaa
     3841 gtcgcggaag tggaagcgtt gtaccgtaaa aatcagatcc cgtggattaa cagtaccaat
     3901 tattcggtag aagagattgc caccaagatc ctcgatatca tgggccttag tcgccgaatg
     3961 tactagagaa ctagtgcatt agcttatttt tttgttatga tgctaaccac ccggcgaggt
     4021 gtgacacacc tcgcacttga aatcagcagc gattggttta tcgtgatgcg catcacttcc
     4081 cggcagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     4141 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     4201 gtaacgcctg gcgccgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactc
     4261 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     4321 gctgcaatgg agtacgcccc ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     4381 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaaggacta
     4441 atctccgatc cagatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     4501 aaattacttt tacaggtaaa tgagctgggc gtcccaaccg cgactgagtt cctcgatatg
     4561 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc acgtactacc
     4621 gaaagtcaga tccaccgtga aatggcttcg gcactctcct gtccggtagg ttttaaaaat
     4681 ggtaccgatg gcaatacgcg gattgctgtg gatgctatcc gcgcagcccg cgccagccat
     4741 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     4801 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     4861 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     4921 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     4981 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgctcgaa
     5041 ggaacgcaaa aaatcgtcgg cagtcagccg ctcacttacg gtcaatccat taccgacccg
     5101 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     5161 cgcttctgaa tgcgtgccca ttcctgacgg aatgggcatt tctgcgcaac ttgttgtctt
     5221 ctcaacaaat tactgcttgc tctggcctg
//
LOCUS       DAZOYS010000241         5180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOYS010000241 DAZOYS010000000
VERSION     DAZOYS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.243"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUN9_003844"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUN9_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0697126.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUN9_003845"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUN9_003845"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0697127.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUN9_003846"
     CDS             2475..4124
                     /locus_tag="ACHUN9_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0697128.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5180)
                     /locus_tag="ACHUN9_003847"
                     /pseudo
     CDS             complement(4278..5180)
                     /locus_tag="ACHUN9_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1120 a         1205 c         1399 g         1456 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg
//
LOCUS       DAZOYS010000242         5177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOYS010000242 DAZOYS010000000
VERSION     DAZOYS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.244"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(200..343)
                     /gene="ypaB"
                     /locus_tag="ACHUN9_003848"
     CDS             complement(200..343)
                     /gene="ypaB"
                     /locus_tag="ACHUN9_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0697129.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            471..2756
                     /gene="nrdA"
                     /locus_tag="ACHUN9_003849"
     CDS             471..2756
                     /gene="nrdA"
                     /locus_tag="ACHUN9_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0697130.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2990..4120
                     /gene="nrdB"
                     /locus_tag="ACHUN9_003850"
     CDS             2990..4120
                     /gene="nrdB"
                     /locus_tag="ACHUN9_003850"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0697131.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4120..4374
                     /gene="yfaE"
                     /locus_tag="ACHUN9_003851"
     CDS             4120..4374
                     /gene="yfaE"
                     /locus_tag="ACHUN9_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0697132.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4428..5077)
                     /gene="inaA"
                     /locus_tag="ACHUN9_003852"
                     /pseudo
     CDS             complement(4428..5077)
                     /gene="inaA"
                     /locus_tag="ACHUN9_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
BASE COUNT         1291 a         1385 c         1263 g         1238 t
ORIGIN      
        1 aacaaaatgg cttcgcattt aacaataacc gaatagaaaa caaccatttc gacatcaaca
       61 atcgcttaca ttcgcttata tattgaccac aactgataca tcagattatg tgatgactcg
      121 tgcttagatc aatttttgca atcattaaca aaaagattaa taaaccatct atatcaattt
      181 atccaaccta ttctgccgtt caagaaatcg ccgaacagtt atttttaaca aatttttctc
      241 ttcccattga ctttcccgga caccttgtct gacctaaggt gcgcgaaagc cactttttcc
      301 ttcctgagtt atccacaaag ttatgcactt gcaagagggt cattttcaca ctatcttgca
      361 gtgaatccca aacatacccc ctatatatag tgttctaagc agcttccctt actacaggta
      421 gtctgcatga aactattgcg gaaagaattc caaaaacagg tacgacatac atgaatcaga
      481 atctgctggt gacaaagcgc gacggtagca cagagcgcat caatctcgac aaaatccatc
      541 gcgttctgga ttgggcggca gaaggactgc ataacgtttc gatttcccag gtcgagctgc
      601 gctcccacat tcagttttat gacggtatca agacctctga catccacgaa accattatca
      661 aggctgccgc agacctgatc tcccgtgatg cgccggatta tcagtatctc gccgcgcgcc
      721 tggcgatctt ccacctgcgt aaaaaagcct acggccagtt tgagccgcct gcgctgtacg
      781 accacgtggt gaaaatggtc gagatgggca aatacgataa tcatctgctg gaagactaca
      841 cggaagaaga gttcaagcag atggacacgt ttatcgatca cgaccgtgat atgaccttct
      901 cttatgctgc cgttaagcag ctggaaggca aatatctggt acagaaccgc gtgaccggcg
      961 aaatctatga gagcgcccag ttcctttata ttctggttgc cgcgtgcctg ttctcgaact
     1021 acccgcgtga aacgcgcctg caatatgtga agcgttttta cgacgcggtt tccacattta
     1081 aaatttcgct gccgacgcca atcatgtccg gcgtgcgtac cccgactcgt cagttcagct
     1141 cctgcgtact gatcgagtgc ggtgacagcc tggattccat caacgccacc tccagcgcga
     1201 ttgttaaata cgtttcccag cgtgccggga tcggcatcaa cgccgggcgt attcgtgcgc
     1261 tgggtagccc gattcgcggt ggtgaagcgt tccataccgg ctgcattccg ttctacaaac
     1321 atttccagac agcggtgaaa tcctgctctc agggcggtgt gcgcggcggt gcggcaacgc
     1381 tgttctaccc gatgtggcat ctggaagtgg aaagcctgct ggtgttgaaa aacaaccgtg
     1441 gtgtggaagg caaccgcgtg cgtcatatgg actacggggt acaaatcaac aaactgatgt
     1501 atacccgtct gctgaaaggt gaagatatca ccctgttcag cccgtccgac gtaccggggc
     1561 tgtacgacgc gttcttcgcc gatcaggaag agtttgaacg tctgtatacc aaatatgaga
     1621 aagacgacag catccgcaag cagcgtgtga aagccgttga gctgttctcg ctgatgatgc
     1681 aggaacgtgc gtctaccggt cgtatctata ttcagaacgt tgaccactgc aatacccata
     1741 gcccgtttga tccggccatc gcgccagtgc gtcagtctaa cctgtgcctg gagatagccc
     1801 tgccgaccaa accgctgaac gacgtcaacg acgagaacgg tgaaatcgcg ctgtgtacgc
     1861 tgtctgcttt caacctgggc gcaattaata acctggatga actggaagag ctggcaattc
     1921 tggcggttcg tgcacttgac gcgctgctgg attatcagga ttacccgatc ccggccgcca
     1981 aacgtggagc gatgggtcgt cgtacgctgg gtattggtgt gatcaacttc gcttactacc
     2041 tggcgaagca cggtaaacgc tactccgacg gcagcgccaa caacctgacg cataaaacct
     2101 tcgaagccat tcagtattac ctgctgaaag cctctaatga gctggcgaaa gagcaaggcg
     2161 cgtgcccgtg gtttaacgaa accacttacg cgaaagggat cctgccgatc gatacctata
     2221 agaaagatct ggataccatc gctaatgagc cgctgcatta cgactgggaa gcattgcgtg
     2281 aatcaatcaa aacgcacggc ctgcgtaact ctacgctttc tgccctgatg ccgtccgaga
     2341 cctcttcgca gatctctaac gccactaacg gtattgaacc gccgcgcggt tacgtcagca
     2401 tcaaagcgtc gaaagacggt attttgcgcc aggtggtgcc ggactacgag cacctgcacg
     2461 acgcctatga gctgctgtgg gaaatgccgg gtaacgatgg ctacctgcaa ctggtaggta
     2521 tcatgcagaa atttatcgat cagtcgatct ctgctaacac caactacgat ccgtcacgtt
     2581 tcccgtcagg aaaagtgccg atgcagcagt tgctgaaaga cctgctcacc gcctacaaat
     2641 tcggggtcaa aacgctgtat tatcagaaca cccgtgacgg cgctgaagac gcgcaggacg
     2701 atctggtacc gtcaatccag gacgatggct gcgaaagcgg cgcatgtaag atctgatatt
     2761 gagatgccgg atgcggcgta aacgccttat ccggcctacg gctcggtttg taggcctgat
     2821 aagacgcgcc agcgtcgcat caggctccgg gtgccggatg cagcgtgaac gccttatccg
     2881 gcctacggct cggatttgta ggcctgataa gacgcgccag cgtcgcatca ggcacaggat
     2941 gcggcgtaaa atgccttatc cggcattaaa ctcccaacag gacacactca tggcatatac
     3001 caccttttca cagacgaaaa atgatcagct caaagaaccg atgttctttg gtcagccggt
     3061 caacgtggct cgctacgatc agcaaaaata tgacatcttc gaaaagctga tcgaaaagca
     3121 gctctctttc ttctggcgtc cggaagaagt tgacgtctcc cgcgaccgta tagattacca
     3181 ggcgctgccg gagcacgaaa aacacatctt tatcagcaac ctgaaatatc agacgctgct
     3241 ggattccatt cagggtcgta gcccgaacgt ggcgctattg ccgcttattt ctattccgga
     3301 actggaaacc tgggtcgaaa cctgggcgtt ctcagaaacg attcattccc gttcctatac
     3361 tcatatcatt cgtaatatcg ttaacgatcc gtctgttgtg tttgacgata tcgtcaccaa
     3421 cgagcagatc cagaaacgtg cggaagggat ctccagctat tacgatgaac tgatcgaaat
     3481 gaccagctac tggcaccttc tgggcgaagg cacccacacc gttaacggta aaaccgtaac
     3541 cgttagcctg cgcgagctga agaaaaaact gtatctctgc ctgatgagcg ttaacgcgct
     3601 ggaagcgatc cgcttctacg tcagctttgc ttgttccttt gcctttgcag aacgcgaact
     3661 gatggaaggt aacgccaaga ttattcgcct gattgcccgc gatgaagccc tgcacctgac
     3721 cggtactcag catatgctga atctgctgcg cagcggcgcg gatgatccgg agatggcgga
     3781 aattgccgaa gaatgtaagc aggagtgcta tgacctgttt gtccaggcgg cacagcaaga
     3841 gaaagactgg gcggattatc tgttccgtga cggttcgatg attggtctga ataaagatat
     3901 tctctgccag tacgttgaat acatcaccaa tatccgcatg caggcagtcg gtttggatct
     3961 gccgttccag acgcgctcca acccgatccc gtggatcaac acctggctgg tgtctgataa
     4021 cgtgcaggtt gctccgcagg aagtggaagt cagttcttat ctggtcgggc agattgacgc
     4081 ggaagtggac accgacgatt tgagtaactt ccagctctga tggcccgcgt taccctgcgc
     4141 atcactggca cacaactgct gtgccaggat gaacaccctt cccttctggc ggcgctggaa
     4201 tcccacaatg ttgcggttga gtaccagtgc cgcgaaggtt actgcggctc ctgtcgcacc
     4261 cgactggtcg caggtcaggt ggactggatt gccgaaccgt tagcctttat tcagccgggg
     4321 gaaattttgc cctgttgttg ccgggcaaaa ggcgatattg aaatcgagat gtgaattggt
     4381 tgtagtgcca gatacaacgc ttatacgtct tatctggcct acaacgatta catggcgtag
     4441 taatacgctt tcacctgctc ccagtccgct ttggggattg gctccagata ttttccagct
     4501 ggcggaagtc atgattaatc gctttattgc gacgcaagcg gcgacgactt ttctccaggt
     4561 caagaaaacc agcttctgct ttaccttctg ttttcacata gatatggcga acataacaac
     4621 agccatgctg acgattaacg ctatgcattt tcttaaacgc cagcgccact gctttcaaca
     4681 tggcttgccg tacttcgtca gaatagggcg atactgcatg ccgggcatac cagtcagcaa
     4741 tgctgatgaa ccccgccata tcttcagtca ccagcaacgc gcgccattca ccctcaattt
     4801 tcaccgcttc accaaaaacg atcttcggca cgatgacacc ggcccgttcc agttctttaa
     4861 ttaccgcaac ctcacggaca atcgttggtc ggccgaacgg ataacgtacg gaatgaaaca
     4921 gatgatgcgt catgcgcttt acatacagct ttttgccgtt gcgctcgacg cattgcaccc
     4981 cgctcatacc attacggcga tagttaggct cttcaaccca gtcgccctct gttgcccacc
     5041 agtgattaaa ttcgtcgtac tttgctgaaa ctgccatacc ctatcgcctg tcatttttat
     5101 taacgacaat gactataggt ggttacctga ggaaaatctt aatgaaacgt gtcgtattaa
     5161 tgaatttctt ttgccgc
//
LOCUS       DAZOYS010000243         5156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOYS010000243 DAZOYS010000000
VERSION     DAZOYS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.245"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(39..401)
                     /locus_tag="ACHUN9_003853"
     CDS             complement(39..401)
                     /locus_tag="ACHUN9_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0697133.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="ACHUN9_003854"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="ACHUN9_003854"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0697134.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHUN9_003855"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHUN9_003855"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0697135.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHUN9_003856"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHUN9_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0697136.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHUN9_003857"
     CDS             complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHUN9_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0697137.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1269 a         1292 c         1361 g         1234 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgt
//
LOCUS       DAZOYS010000244         4939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOYS010000244 DAZOYS010000000
VERSION     DAZOYS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.246"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUN9_003858"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUN9_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHUN9_003859"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUN9_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUN9_003860"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUN9_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUN9_003861"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUN9_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0697138.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUN9_003862"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUN9_003862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUN9_003863"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUN9_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0697139.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUN9_003864"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUN9_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0697140.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1194 a         1148 c         1160 g         1437 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaact
//
LOCUS       DAZOYS010000245         4933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOYS010000245 DAZOYS010000000
VERSION     DAZOYS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.247"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            227..1171
                     /gene="leuO"
                     /locus_tag="ACHUN9_003865"
     CDS             227..1171
                     /gene="leuO"
                     /locus_tag="ACHUN9_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0697141.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1326..1370)
                     /locus_tag="ACHUN9_003866"
     CDS             complement(1326..1370)
                     /locus_tag="ACHUN9_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697142.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1489..3213
                     /gene="ilvI"
                     /locus_tag="ACHUN9_003867"
     CDS             1489..3213
                     /gene="ilvI"
                     /locus_tag="ACHUN9_003867"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0697143.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3216..3707
                     /gene="ilvN"
                     /locus_tag="ACHUN9_003868"
     CDS             3216..3707
                     /gene="ilvN"
                     /locus_tag="ACHUN9_003868"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0697144.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3719..3805
                     /locus_tag="ACHUN9_003869"
     CDS             3719..3805
                     /locus_tag="ACHUN9_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0697145.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3887..4891
                     /gene="cra"
                     /locus_tag="ACHUN9_003870"
     CDS             3887..4891
                     /gene="cra"
                     /locus_tag="ACHUN9_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0697146.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1199 a         1120 c         1300 g         1314 t
ORIGIN      
        1 ttagattgtt ttcgcaacaa aaacattatg gattcttatg ctgtggtaaa tgactcattc
       61 cacggcaatg gattctgttt ttatcagaac ccgtatcttt atgttttccg aattttactc
      121 attttgcttt ttcttatttt atatgcatga taaatcatat tcttcaggat tatttctctg
      181 cattccaata agggaaaggg agttaagtgt gacagtggag ttaagtatgc cagaggtaca
      241 aacagatcat tcagagacgg cggagttaag caagccacag ctacgcatgg tcgatctcaa
      301 cttattaacc gttttcgatg ccgtgatgca ggagcaaaac attacccgtg ccgctcatgt
      361 tctgggtatg tcgcaacctg cggtcagtaa cgctgttgca cgcctgaagg tgatgtttaa
      421 tgacgagctt tttgttcgtt atggccgtgg tattcaaccg actgctcgcg catttcaact
      481 ttttggctca gttcgtcagg cattgcaact agtacaaaat gaattgcctg gttcaggttt
      541 tgaacccgcg agcagtgaac gtgtatttca tctttgtgtt tgcagcccgt tagacagcat
      601 tctgacctcg cagatttata atcacattga gcagattgcg ccaaatatac atgttatgtt
      661 caagtcttca ttaaatcaga acactgaaca tcagctgcgt tatcaggaaa cggagtttgt
      721 gattagttat gaagacttcc atcgtcctga atttaccagc gtgccattat ttaaagatga
      781 aatggtgctg gtagccagca aaaatcatcc aacaattaag ggcccgttac tgaaacatga
      841 tgtttataac gaacaacatg cggcggtttc gctcgatcgt ttcgcgtcat ttagtcaacc
      901 ttggtatgac acggtagata agcaagccag tatcgcgtat cagggcatgg caatgatgag
      961 cgtacttagc gtggtgtcgc aaacgcattt ggtcgctatt gcgccgcgtt ggctggctga
     1021 agagttcgct gaatccttag aattacaggt attaccgctg ccgttaaaac aaaatagcag
     1081 aacctgttat ctctcctggc atgaagctgc cgggcgcgat aaaggccatc agtggatgga
     1141 agagcaatta gtctcaattt gcaaacgcta actgaatgca gaataggtca gacatgaatg
     1201 tctggtttat tctgtatttt ttattgaatg tagaatttta ttctgaatgt gtgggctctc
     1261 tattttagga ttaattaaaa aaatagagaa attgctgtaa gttgtgggat tcagccgatt
     1321 tattatcaat ttaatccttc gtaatggagg attttatcgt ttcttttcac ctttcctcct
     1381 gtttattctt attaccctgc gtttatgtct ctggctgcca attgcttaag caagatcgga
     1441 cggttaatgt gttttacaca ttttttccgt caaacagtga ggcaggccat ggagatgttg
     1501 tctggagccg agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca agtattcggt
     1561 tatcccggag gcgcagtcct tgatatttac gatgcattgc ataccgtggg tggtatcgat
     1621 catgttttag ttcgtcatga gcaggcggca gtgcatatgg ccgatggtct ggcgcgcgcg
     1681 accggggaag tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa tgcgattact
     1741 ggcatcgcca ccgcttatat ggattccatt ccattagttg tcctttccgg gcaggtagcg
     1801 acctcgttga taggttacga tgcctttcag gagtgcgaca tggtggggat ttcgcgacca
     1861 gtggttaaac acagttttct ggttaagcaa acggaagaca ttccgcaggt gctgaaaaag
     1921 gctttctggc tggcggcaag tggtcgtcct ggaccagtag tcgttgattt accgaaagat
     1981 attcttaatc cggcgaacaa attaccctat gtctggccgg agtcggtcag tatgcgttct
     2041 tacaatccca ctactaccgg acataaaggg caaattaagc gcgctctgca aacgctggta
     2101 gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa tcacggcggg ctgccatcag
     2161 cagttgaaag aaacggtgga ggcgttgaat ctgcccgttg tttcctcatt gatggggctg
     2221 ggagcgtttc cggcaacgca tcgtcaggca ctgggcatgc tgggaatgca cggtacctac
     2281 gaagccaata tgacgatgca taacgcggat gtgattttcg ccgtcggggt acgatttgat
     2341 gaccgaacga cgaacaatct ggcaaagtac tgcccaaatg ccactgttct gcatatcgat
     2401 attgatccta cttccatttc taaaaccgtg actgcggata tcccgattgt gggggatgct
     2461 cgccaggtcc tcgaacaaat gcttgaactc ttgtcgcaag aatccgccca tcaaccactg
     2521 gatgagatcc gcgactggtg gcagcaaatt gaacagtggc gcgctcgtca gtgcctgaaa
     2581 tatgacactc acagtgaaaa gattaaaccg caggcggtga tcgagactct ttggcggttg
     2641 acgaagggag acgcttacgt gacgtccgat gtcgggcagc accagatgtt tgctgcactt
     2701 tattatccat tcgacaaacc gcgtcgctgg atcaattccg gtggcctcgg cacgatgggt
     2761 tttggtttac ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac cgtggtttgc
     2821 gtcactggcg acggcagtat tcagatgaac atccaggaac tgtctaccgc gttgcaatac
     2881 gagttgcccg tactggtggt gaatctcaat aaccgctatc tggggatggt gaagcagtgg
     2941 caggacatga tctattccgg ccgtcattca caatcttata tgcaatcgct acccgatttc
     3001 gtccgtctgg cggaagccta tggtcacgtc gggatccaga tttctcatcc gcatgagctg
     3061 gaaagcaaac ttagcgaggc gctggaacag gtgcacaata atcgcctggt gtttgttgat
     3121 gttaccgtcg atggcagcga gcacgtctac ccgatgcaga ttcgcggggg cggaatggat
     3181 gaaatgtggt taagcaaaac ggagagaacc tgattatgcg ccggatatta tcagtcttac
     3241 tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct tttttcccag cgtggctaca
     3301 acattgaaag cctgaccgtt gcgtcaaccg atgatccgac attatcgcgt atgaccatcc
     3361 agaccgtggg cgatgaaaaa gtacttgagc agatcgaaaa gcaattacac aagctggtcg
     3421 atgtcttgcg cgtgagtgag ttggggcagg gcgcgcatgt tgagcgggaa atcatgctgg
     3481 tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg gaaatattcc
     3541 gtgggcaaat tatcgatgtc acaccctcgc tttataccgt tcaattagca ggcaccagcg
     3601 ataagcttga tgcattttta gcatcgattc gcgaggtggc gaaaattgtg gaagttgctc
     3661 gttctggtgt ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa tgatctgaat
     3721 gcgcaatttg cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag tgggaacctg
     3781 gaataaaagc agttgccgca gttaatttta tgcgcttaga tgttaatgaa tttaacccat
     3841 accagtacaa tggctatggt ttttacattt tacgcaaggg gcaattgtga aactggatga
     3901 aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc tatgttatta acggcaaagc
     3961 gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg tgcgtgagca
     4021 caattaccac ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac gttctattgg
     4081 tcttgtgatc cccgatctgg agaacaccag ctatacccgc atcgctaact atcttgaacg
     4141 ccaggcgcgg caacggggtt atcaactgct aattgcctgc tcagaagatc agccagacaa
     4201 cgaaatgcgc tgtattgagc accttttaca gcgtcaggtc gatgccatta ttgtttcgac
     4261 gtcgttgcct cctgagcatc ctttttatca acgctgggct aacgacccgt tcccgattgt
     4321 cgcgctggac cgcgccctcg atcgtgaaca cttcaccagc gtggttggtg ccgatcagga
     4381 tgatgccgaa atgctggcgg aagagttacg taagtttccc gccgagacgg tgctttatct
     4441 tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa caaggtttcc gtactgcctg
     4501 gaaagatgat ccgcgcgaag tgcatttcct gtatgccaac agctatgagc gggaggtggc
     4561 tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc tgttcacaac
     4621 gtcgtttgcg ttgttgcaag gggtgatgga tgtcacgcta cgtcgcgatg gtaaactgcc
     4681 ttctgacctg gcaattgcca cctttggcga taacgaactg ctcgacttct tacagtgccc
     4741 ggtgctggca gtggctcaac gtcaccgcga tgtcgcagag cgtgtgctgg agattgtcct
     4801 ggcaagcctg gacgaaccgc gtaagccaaa acctggttta acgcgcatta aacgtaatct
     4861 ctatcgccgc ggtgtgctca gccgtagcta agccgcgaac aaaaatacgc gccaggtgaa
     4921 tttccctctg gcg
//
LOCUS       DAZOYS010000246         4907 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOYS010000246 DAZOYS010000000
VERSION     DAZOYS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.248"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(278..841)
                     /locus_tag="ACHUN9_003871"
     CDS             complement(278..841)
                     /locus_tag="ACHUN9_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697147.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            1006..1257
                     /locus_tag="ACHUN9_003872"
     CDS             1006..1257
                     /locus_tag="ACHUN9_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0697148.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKVS"
     gene            1259..3355
                     /locus_tag="ACHUN9_003873"
     CDS             1259..3355
                     /locus_tag="ACHUN9_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0697149.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRRVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VRQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            3431..4381
                     /locus_tag="ACHUN9_003874"
     CDS             3431..4381
                     /locus_tag="ACHUN9_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0697150.1"
                     /translation="MTDIDDVFNTINALINEGDKSQEDIADEAGMSPATLSALRKGEY
                     NGNVEGMHDKLQKWYQVWQKGQNLSGTPGVVKTQTFCDLHELFKTVRSLGIISVVVGV
                     PGVGKTFAAREYCRRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAI
                     RRKLGGGKETSGRVNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADAT
                     RRGTDELARLFSRFAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRV
                     LTHTLNHAWLAAKGAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            4397..4624
                     /locus_tag="ACHUN9_003875"
     CDS             4397..4624
                     /locus_tag="ACHUN9_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697151.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
     gene            4657..>4907
                     /locus_tag="ACHUN9_003876"
     CDS             4657..>4907
                     /locus_tag="ACHUN9_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697152.1"
                     /translation="MAKVIIEIKNTVSGIKGLNLRTSIAVDGSAELDGDEGTLAGMVA
                     LLVLNKSQKIINESAHEAIEILKNDSVITGGRVTEMAVE"
BASE COUNT         1382 a         1078 c         1280 g         1167 t
ORIGIN      
        1 ttgtatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag acgggaatag
       61 attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc aaaaatgtta
      121 aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa tagcctttaa
      181 agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa gcgccaaaac
      241 caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct gctttctgag
      301 gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta accttttcgt
      361 ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga gcgtgcaact
      421 cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc ccaagaacat
      481 cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga atttgagcat
      541 caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca acatcaccct
      601 ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt tttaactgat
      661 gcgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa gtgccttcat
      721 tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact aacctaaaga
      781 ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt gcttttctca
      841 ttcttggttc ctttctatct aattcattga taaataatga aattaaaaaa taaaccataa
      901 aaacaccaaa gaacccttta caaggaatct ttggtatctt aatctttatc catacggata
      961 tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag acaaagagaa
     1021 gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga taacgggtgg
     1081 tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact gaaagcggta
     1141 gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg tgtaccgcca
     1201 gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggt gtcgtgatat
     1261 gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag ccaccacaaa
     1321 aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta gtcgccgtcg
     1381 ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa cacgaaaggc
     1441 actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt ctgaagtgaa
     1501 agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag catatcgcgg
     1561 ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc gccgggtatc
     1621 tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg gtaacctcgg
     1681 gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc tgccggagcg
     1741 cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta ctatcagccg
     1801 cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg ccgaacggct
     1861 gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca gctggctgcc
     1921 tgaatttctg gcgcagtatc ggcaggtgaa cggcagaccc atgtcagaag cctacgagga
     1981 ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg aggtgatgcc
     2041 ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga gacaaaaagg
     2101 gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg actggacagc
     2161 tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga aatgtgcgca
     2221 tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg gtggaacacg
     2281 gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg ccggtgcgat
     2341 acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata acggttccgg
     2401 tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc tggggattaa
     2461 acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac gcctgaaccg
     2521 gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg gtgctgaccg
     2581 ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc tgcaacagga
     2641 caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt gggcggagct
     2701 tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt ctgagctgcc
     2761 gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac gcctggcaga
     2821 agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt tccgcccaat
     2881 ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt actacgcgcc
     2941 ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc atgacgccga
     3001 gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat ggaacggcaa
     3061 taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc gtattaaagg
     3121 aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta ttcagattct
     3181 ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg gtaatgtggt
     3241 ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac gcgatttccg
     3301 gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg cctgaataaa
     3361 aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt gaaataaccg
     3421 aggaaatact atgactgata ttgatgatgt tttcaacact attaacgctt tgattaacga
     3481 gggcgacaag tctcaggaag atattgctga tgaagccgga atgtctccgg caacactctc
     3541 ggctctgcgt aaaggagagt acaacggtaa tgtcgaaggt atgcatgaca aattacagaa
     3601 atggtatcag gtctggcaga agggacagaa cttgtcaggt acccctggtg ttgtgaagac
     3661 ccagacattt tgtgatttgc atgagctgtt taaaacagtg cgctcactcg ggattatcag
     3721 tgtcgttgtt ggcgtaccgg gtgtcggaaa aacctttgct gcccgggaat attgtcgcag
     3781 gacagctaat gcctggatgg tgactcttgc tccggcacat tcgagcgtaa cggaatgcct
     3841 ccttgaaata gcatatgcgc tggaaattaa taatccggga agaaataagg ggaatataac
     3901 ccgcgccatt cgcagaaaac tgggtggtgg taaagaaacc agtggccgtg ttaaccccct
     3961 tctgattatt gatgaagctg atcacttaag tgtggatggt ctggagcaat tacgcgccat
     4021 tcaggatgcc actggcgtgg ggatggtttt aattggcaac ccgaaacaga tggcggatgc
     4081 cacccgtcgt gggactgatg aactggcccg tctgttcagc cgttttgcca tgacaaaaca
     4141 actgcgcaaa gtaaaaaaga ccgatgtgat ggctgtagcc aaagcatggg gagtaacaca
     4201 ggaagatgag ctggacctgc tgatgagggt agccgaaaag cccgggggat tgcgtgtatt
     4261 aactcacaca ctgaatcatg cgtggctggc ggcaaaaggt gcaggcacat ccctgaataa
     4321 aagtcacatt aaagcggctt ttaaggaggc ttacagcaac cccaggttac tgaactggta
     4381 gtaaaggaca taaaccatga tgtcacgaaa tataaaaatg gcaacggatg cgcagaactg
     4441 gttacaggcg cacgggagtc acgtaaatga atcatatctc ggcgtggcgc gtccggtact
     4501 tgaaatcacc tgcccaccac cggagctggt cagaaacgct gtcaggatta tggagcataa
     4561 atcaggggtt gcccgttcag tatggacggc ccgccttaat ggctgccaga ttatctggcg
     4621 ttaataacgg cttatttatt tgtgaggaat aacaaaatgg caaaagtaat tattgaaatt
     4681 aaaaatacag tatctggaat taaaggactg aatttgcgca ccagcattgc ggtggacggt
     4741 agtgcggaac tggacggtga tgagggtaca cttgctggca tggtggcgtt actggtgctt
     4801 aataaaagcc agaaaataat taatgagtca gcccatgaag ctatcgagat tttgaaaaac
     4861 gatagtgtca tcaccggtgg ccgtgtcact gaaatggctg ttgaaaa
//
LOCUS       DAZOYS010000247         4887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOYS010000247 DAZOYS010000000
VERSION     DAZOYS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.249"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..127
                     /locus_tag="ACHUN9_003877"
     CDS             <1..127
                     /locus_tag="ACHUN9_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0697153.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
     gene            198..695
                     /gene="rayT"
                     /locus_tag="ACHUN9_003878"
     CDS             198..695
                     /gene="rayT"
                     /locus_tag="ACHUN9_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0697154.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(818..2530)
                     /locus_tag="ACHUN9_003879"
                     /pseudo
     CDS             complement(818..2530)
                     /locus_tag="ACHUN9_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2502..3287
                     /gene="lafU"
                     /locus_tag="ACHUN9_003880"
     CDS             2502..3287
                     /gene="lafU"
                     /locus_tag="ACHUN9_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0697155.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3358..4413
                     /gene="dinB"
                     /locus_tag="ACHUN9_003881"
     CDS             3358..4413
                     /gene="dinB"
                     /locus_tag="ACHUN9_003881"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0697156.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            4410..4862
                     /locus_tag="ACHUN9_003882"
     CDS             4410..4862
                     /locus_tag="ACHUN9_003882"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0697157.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
BASE COUNT         1176 a         1280 c         1299 g         1132 t
ORIGIN      
        1 gcacatcgct attaatattt tgacgaatga taaggtattc aaggcagggt taagacgtaa
       61 gatgcgaaaa gcagccatgg acagaaacta tctggcgtca gtccttgagg ggagcgggct
      121 ttcgtaatct tgccctgttc catcacctca ttgccgacct ttccttttct tcttaccgtt
      181 gagaaaaagg agtcgccatg tctgaatatc gtcgttatta catcaagggg ggaacctggt
      241 ttttcacggt gaatttacgc aatcgtcgaa gccaactctt gaccacccag taccagatgc
      301 tccgtaacgc cattattaaa gttaagcgag acaggccttt tgaaatcaac gcctgggtcg
      361 ttttgccaga gcatatgcat tgcatatgga cattacctga aggcgatgat gatttttcct
      421 ctcgctggcg ggaaattaaa aagcaattta cccatgcttg tggattgaaa aatatctggc
      481 aaccacgttt ttgggagcac gccatccgca ataccaaaga ttataggcat catgttgatt
      541 atatttatat aaatccagta aagcatggtt gggtaaagca agtgagtgat tggccattct
      601 caacgttcca tcgcgatgtc gcgcgagggt tatatcccat cgattgggcg ggggacgtaa
      661 cggatataaa tgctggcgag cgtatcattt tataattatg cgcagatgcc tgatgcgacg
      721 ctggcgcgtc ttatcatgcc tacaaacttg tgcctgaacg gtaggccgga taaggcgttt
      781 acgccgcatc cggcagttgt gcaccaatgc cggatactta catcaacgcc cccataatct
      841 tcaactccag ctcgtccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca
      901 accttgcata acgcgccagc aaagggcgca gctgcggtgg caccagcagc accgggtctt
      961 tccccgccgc tttcatctgc tctttcacct gtggcatcgt gctctggaac tggttgagca
     1021 tattcgggtc cactggcacg ctgtcgagca tcactttacc gccctgttgc gcctgattca
     1081 ctaggttagt cagcagattt tccagctcat tattcagggt atacaccgtc agctcctgct
     1141 tgcgaacgaa cggatgggta atgctgcgcc gcagcgccag gcgcacatcg gctgccagca
     1201 gaatatgatc tttggtcacc gcgctactgg cgaccagcac ggtggcgatg gtgacgatat
     1261 cgcgcaggga aacgccttcg gtcagcagcg cccggtacac tttcagcaac tggctgtaat
     1321 tgagcaccgc gcttaaatcc tccgccaggc gcggtgccat cgacgacaaa cggttatgca
     1381 actgcgtaat atcgtcatag ttaaataaat caggaatata gctgcgcaca atcttattta
     1441 catgggtagc aatcacgctg gcgctgtcga tcacctgata ccccatattc agcgccttcg
     1501 ctttctgcgc aggctggatc caggtcaccg gcatcccgta cgccgggtcg ttccccagca
     1561 cgccgtctat ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa
     1621 tatccgcttc atcagcctta atgccgttga tgaaaatagc gtactggctg ggcttaaggc
     1681 ggaagttttc ccgaatgcgg atctccggca gcagcacgcc gttgccgtcg gatatcacct
     1741 gtcgcacgcc gcgaatacgc tgggtgagcg ggttgccctg ggctttgtcc accagcgcca
     1801 ccagcttgta gccgaggctt aagctgatgg gctcgatcag cggaatggtt tcccaactga
     1861 cctgttgctc gctggtttcg gtgatggtgc gggtcagcgt ttcgagactt ttctcttccg
     1921 cttccgccgc ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggcgctga
     1981 acagcaaaaa cggcagatgt ggcattcccg gcactaccgc cagtacgaac ataattccgg
     2041 tagcggtgta gagcaccgat gggcttgcca gcaactggtt acgcacgtca tgggcgatat
     2101 cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc ggtggagagt agcagggaag
     2161 ggatttgcgc caccagcccg tcgccgatgg tcatcagcac atactgctgg aaggcggcgt
     2221 cggcgctcag gttgtatttg aagatcccga tacagacgcc gccgatcagg ttgatcgcca
     2281 gaatcatcat cccggcgata gcatccccgc gcacaaattt cgatgccccg tccatcgcgc
     2341 cgtagaaatc ggcctcgctg gcaacatctt tacgtcgggt ttgtgcctgc gcctgattga
     2401 tcaacccggc attaagatcg gcgtcaatcg ccatctgctt accgggcatc gcgtccaggg
     2461 taaagcgggc agaaacctcg gagatacgct cggccccttt ggtgacgaca ataaagttga
     2521 ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt
     2581 cgatttttaa cggtggcggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa
     2641 aacctgccac cgtggcggtg ccggaagaaa cggagaaaaa agcgcgcgac gtcaatgaga
     2701 aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagctggca accagtatca
     2761 ccaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg
     2821 gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc
     2881 agattatgcc gttctttaaa acgctgctgg tggagctcgc gccagttttc gactcgctcg
     2941 ataacaaaat tattattacc gggcataccg atgcgatggc ttacaaaaac aatatctaca
     3001 acaactggaa cctttcgggc gatcgcgcgc tttcggctcg tcgggtgctg gaagaggccg
     3061 gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggatt
     3121 ccaaaaatcc gcaaagcgcg ggcaaccggc gcattgagat tatggtgctg accaaaagtg
     3181 cgtccgatac gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg
     3241 tgcaaaagct ggataagcag caggtgcttt cgcagcgaac gcgttaaatg ctgaatcttt
     3301 acgcatttct caaactctga aatcactgta tactttacca gtgttgagag gtgagcaatg
     3361 cgtaaaatca ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat
     3421 cccgccctgc gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc
     3481 agtaccgcca attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc gacagggatg
     3541 gcgctcaaat tatgcccgca tctcaccttg cttccggggc gctttgacgc ctacaaagaa
     3601 gcctcaaatc atatccgcga aatcttctcg cgctacacct cgcgtattga accgttgtca
     3661 ctggatgagg cttatctcga cgtcaccgat agcgtccatt gccacggttc tgcgaccctc
     3721 atcgcccagg aaatccgcca gacgattttc aacgagctgc aactgacggc gtctgcgggc
     3781 gtggcacccg taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggccag
     3841 tttgtgatta cgccggcaga agttccggca tttttacaaa ccttaccact ggcaaaaatc
     3901 cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga tggggctacg aacctgcggt
     3961 gatgtacaaa agtgtgatct ggtgatgctg cttaaacgct ttggcaaatt tggccgcatt
     4021 ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttgcgaaaa
     4081 tccgtcggcg tggaacgcac gatggcggaa gatatccacc actggtctga atgtgaagcg
     4141 attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta
     4201 ctgattgccc gccagggggt gaaattaaag ttcgacgatt ttcagcaaac cactcaggag
     4261 cacgtctggc cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat
     4321 gaacgccgcg gcgggcgcgg tgtgcgactg gtggggctgc atgtgacgtt gcttgatccg
     4381 caaatggaaa gacaactggt gctgggatta tgaataacat acaaataaga aactatcagc
     4441 ctggcgattt tcagcaacta tgcgctattt tcattagagc ggttacgatg accgccagtc
     4501 agcattattc tccacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg
     4561 agaaactcgc gaaatcacaa gtgtgggttg cggtcattaa tgcacaaccg gttggtttta
     4621 tttcccgcat tgaacattat atcgatatgt tatttgttga ccctgaatac acccgccgcg
     4681 gggttgccag cgccttgtta aaaccattta ttaagtctga atccgaactt acggtagatg
     4741 ccagcataac cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcagc
     4801 acgttgaatg ccggggagcg tggtttacta atttttatat gcaatataaa ccgcaacatt
     4861 aaatccagct tgcaatgaaa ataacgc
//
LOCUS       DAZOYS010000248         4867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOYS010000248 DAZOYS010000000
VERSION     DAZOYS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.250"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(149..3370)
                     /gene="carB"
                     /locus_tag="ACHUN9_003883"
     CDS             complement(149..3370)
                     /gene="carB"
                     /locus_tag="ACHUN9_003883"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0697158.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3388..4536)
                     /gene="carA"
                     /locus_tag="ACHUN9_003884"
     CDS             complement(3388..4536)
                     /gene="carA"
                     /locus_tag="ACHUN9_003884"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0697159.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1093 a         1358 c         1250 g         1166 t
ORIGIN      
        1 aagtgtgagc taaatcctgc ttatgtcatc aaaaatatcg attccccaaa agatttactt
       61 aatgggcaaa aaaaacggtc ctcatcagag aaccgtatta cttattcgat caaattagcg
      121 gatgaaaaat atccgccatg acacgctatt atttgatctg cgcgtgcatt tcctgcaccg
      181 aaattacttt ttcggtcgcg tcggcattca gcgccatcgc ggtagcgaaa ccaccgttca
      241 gggtggtgtc ataatgcact ttatattgca gcgcactgcg acggatcacg cgggagtctt
      301 caatcgcgcg acggcctgaa gtggtgttga tgatgtaggt atattcgcca ttcttgatgc
      361 ggtcctgaat gtgcggacgg ccttcatgca ccttgttcac cagacgcgga ttaatgcccg
      421 cttcgcccag cacaatcgcc gtgccgtggg tcgcatccag ctcgaagccc tgtttcagca
      481 gttttgccgc caggtccacc acgcgttctt tatcgccttc gcgcacggaa agcaacgcac
      541 gaccgtgttt cttcatggtg gagttgctgc ccagctgcgc tttggcaaac gcttcagcga
      601 aggtgcggcc cacgcccatc acttccccgg tggagcgcat ttctggccct aacagcgggt
      661 caacacccgg gaatttattg aacggcagca ccacttcttt caccgagtag tacggcggga
      721 taacttcttt ggttacgccc tgctcagcca gcgatttgcc agccatcaca cgcgccgcca
      781 cttttgccag cggtacgccg gtggctttgg agacgaacgg aacggtacgc gccgcacgcg
      841 ggttaacttc aatcaggtag acttcgttgt ttttcaccgc aaactgcacg ttcatcagac
      901 cgcgcacctg caattcgaag gccagtttct gcacctgctg gcgcatcaca tcctgaattt
      961 cctgacttaa ggtgtaggct ggcagagaac atgcggagtc accggagtgc acgcccgcct
     1021 gctcgatgtg ctccatgatg ccgccaatca gcaccatttc gccgtcgcag atggcatcca
     1081 cgtcaacttc taccgcgtca tcaaggaagt gatccagcaa cactggcgca tcgttagaca
     1141 cgctgaccgc cgtctggaag tagcgacgca ggtcagcttc gtcatagacg atttccatcg
     1201 cccgaccgcc gagaacgtaa gacggacgta ccaccagtgg gtagccaatc tctttcgcct
     1261 tctctaccgc catttcaata gcggtaacgg tggcgttcgc cggttgtttc agtttcagac
     1321 gctcaaccgc atgctggaag cgttcacggt cttctgcacg gtcgatagca tccgggctgg
     1381 tgccgataac cggtacgcca gcagcttcca gcgcgcgcgc cagtttcagc ggggtctgac
     1441 cgccgtactg aacgataacg cctttcggct tctcaatacg cacgatttcc agcacatctt
     1501 ccagagttac cggctcgaag tagaggcgat cggaagtgtc gtagtcggtg gagacggttt
     1561 ccgggttaca gttaaccata atggtttcgt aaccgtcttc gcgcagcgcc agcgaggcgt
     1621 gtacacagca gtagtcgaat tcgataccct gaccgatacg gttcgggccg ccgccgagaa
     1681 ccatgatttt ttcacggtcg gtagacggat tcgcttcgca ctcttcttca taagtggagt
     1741 acatgtaagc ggtgtcggtg gcgaactctg ccgcacaggt atccacgcgc ttataaaccg
     1801 ggtgcaggtc atactggtcg cgcagcttgc ggatttccgc ttcgcgcacg cccgccagtt
     1861 ttgccagacg cgcatcggca aagcctttgc gtttcagctg gcgcaggaat tcagcgttca
     1921 ggccagtgat gcccacttcc gctactttct cttccagacg caccagttct tcaatctgta
     1981 ccaggaacca gcggtcaata ttggtcaggt tgaacacgcc gtccacagac aggcccgcac
     2041 ggaaggcatc ggcgatgtac cagatacgct ctgcgccagc atctttcagt tcgcgacgga
     2101 ttttggttaa cgcttccggg tcatcgaggc tcactttcgg gtcgaatcca gtcgcaccga
     2161 cttccaggcc gcgcagcgct ttttgcaggg attcctgctg cgtgcgacca atcgccatca
     2221 cttcgccaac cgatttcatc tgagtggtca gacggtcgtt agcaccagcg aatttttcga
     2281 agttgaagcg aggaatttta gtaaccacat agtcgatgga cggctcgaag gaggccggag
     2341 tacgtccgcc agtgatgtcg ttcatcagtt cgtcgagggt gtaacccacc gccagtttcg
     2401 ccgccacttt agcaatcggg aaaccggtcg ctttcgacgc cagcgccgaa gaacgggaca
     2461 cgcgtgggtt catttcgata acaatcaggc gaccgttttt cgggttcacc gcaaactgga
     2521 cattggaacc accggtttca acgccgattt cacgcagcac cgccatcgag gcgttacgca
     2581 tgatttgata ttctttgtcg gtcagcgttt gggctggcgc gacagtgatg gagtcaccgg
     2641 tatggatgcc catcgcatca aagttttcga tagagcagac gatgatgcag ttgtcgtttt
     2701 tatcacgcac cacttccatc tcgtactctt tccagccgat cagcgactca tcaatcagca
     2761 gctctttggt tggggagaga tccagaccgc gggcgcaaat ttcttcaaac tcttcgcggt
     2821 tataagcgat accgccgccg ctaccgccca tggtaaagga tgggcgaata atgcacggga
     2881 agcccacgtc agcggcaacc gccagcgctt cttccatcgt gtgtgcgata ccggaacgcg
     2941 cggtttccag accaattttc ttcatcgcta cgtcgaaacg acggcggtct tctgctttat
     3001 caatcgcatc ggcggtcgca ccaatcatag tcacgccgaa ctcttccaac acgccctgac
     3061 gctccagctc cagcgcgcag ttcagcgccg tctgaccgcc catcgttggc agcaccgcgt
     3121 ccgggcgctc tttttcaata atcttgcgta ctacttccca gtgaatcggc tcgatgtagg
     3181 tcgcatcggc catttccggg tcggtcatga tggtcgccgg gttggagttc accagaatga
     3241 cgcggtaacc ctcttcgcgc agggctttac atgcttgcgc gccagagtag tcaaactcac
     3301 acgcctgacc gataacaatc gggcccgcac ccagaatcag gatacttttt atatctgtac
     3361 gttttggcat ggctctttta ctcctgatta cttagcggtt ttacggtact gctcaattaa
     3421 ctcgataaag tggtcgaaca acggcgctgc gtcgtgtggc ccagggctgg cttccgggtg
     3481 accctggaag ctgaacgccg gtttatcagt gcgatgaatg ccctgtaacg taccgtcgaa
     3541 cagggattta tgcgtgacac gcaggtttgc aggtaatgtt gcttcatcca ccgcaaaacc
     3601 gtggttctgg gcggtgatca tcaccacgtt tttctccaca tctttaaccg gatggttgcc
     3661 gccgtggtga ccaaatttca ttttgacagt cttcgcaccg ctcgccagcg ccagcagctg
     3721 atgaccgagg cagatgccga ataccggaat atcggtttcg aggaatttct ggatggcggt
     3781 aatggcgtaa tcgcacgggg ccgggtcgcc aggaccgttg gagaggaaga tgccgtctgg
     3841 attcattttc agcacatctt ccgcagaagt ttgcgccgga acgatggtca ggcgacagcc
     3901 tctatccacc agcatccgca ggatgttgcg cttggcacca aaatcataag ccacgacgtg
     3961 gaacggcagc tcgtcttctt ttttcgcttc tggcaggccg ccggtcaacg tccagctccc
     4021 ttgtgtccag ctataggctt ctgcggtggt cacttctttt gccagatcca tgccattcag
     4081 acctgggaac gcgcgggctt tttctaacgc caacgccgca tccgggttat cgcctgcgat
     4141 aatgcagcca ttctgtgcgc ctttctcgcg cagtaaacgc gtcagcttac gggtatcgat
     4201 atcggcaatc gccacgatgt tatggcgctt caggtaagaa gagaggtctt cggtattacg
     4261 gaagttgctg gcaatcagcg gcagatcgcg aatcaccaga ccttgtgcat gtacctgaga
     4321 agattcttca tcggcgtcat tagtgccgac attgccaata tggggataag taagagtaac
     4381 gatttgacga gaataggaag gatcagtgag gatttcttga taaccggtca ttgaagtatt
     4441 gaaaacgact tccccaaccg ccgaacctgt tgcccctatg gcccgaccgt gaaactgggt
     4501 tccgtcttcc agaaccaata gcgctgactt aatcaaaaca ccctccagag aatattcact
     4561 cactttattt gcatattaat tcacaatgat gacgtaaatc aatgcaaatc tgcttaccga
     4621 cgaatttctg gcaaacggcg gcattctgga gatatgggcg ctaaaagtca acttaatggc
     4681 ggtatttttt atattatttt gtggctcatg catgttttac aggcattaag cgacaaaaag
     4741 atcatataac ctgttctgga aaacgcttgc gcagcaagaa gattgcatga aataaaaacg
     4801 agcaggaaaa agtggaccaa atggtcaaaa tttacattaa aacaacaaaa acagaagaca
     4861 aaacatg
//
LOCUS       DAZOYS010000249         4835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOYS010000249 DAZOYS010000000
VERSION     DAZOYS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.251"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..222
                     /locus_tag="ACHUN9_003885"
     CDS             <1..222
                     /locus_tag="ACHUN9_003885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697160.1"
                     /translation="YDNPLFFCPFFAPSTAFCPIFAINNFPHKVISKPNSCSTFFLNI
                     MSPAGNNLIPVLGLKAVNDNHFHFVWVML"
     gene            276..1190
                     /gene="sitA"
                     /locus_tag="ACHUN9_003886"
     CDS             276..1190
                     /gene="sitA"
                     /locus_tag="ACHUN9_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0697161.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1190..2017
                     /gene="sitB"
                     /locus_tag="ACHUN9_003887"
     CDS             1190..2017
                     /gene="sitB"
                     /locus_tag="ACHUN9_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0697162.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            2014..2871
                     /gene="sitC"
                     /locus_tag="ACHUN9_003888"
     CDS             2014..2871
                     /gene="sitC"
                     /locus_tag="ACHUN9_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0697163.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2868..3725
                     /gene="sitD"
                     /locus_tag="ACHUN9_003889"
     CDS             2868..3725
                     /gene="sitD"
                     /locus_tag="ACHUN9_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0697164.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(3926..4549)
                     /locus_tag="ACHUN9_003890"
     CDS             complement(3926..4549)
                     /locus_tag="ACHUN9_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0697165.1"
                     /translation="MLMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQRIWQELQSGKWGEIAPFTVTPEMLEAAREARRQGIEAW
                     RTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIVRDVMTWGDADNQQVKLSM
                     PELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(4500..>4835)
                     /locus_tag="ACHUN9_003891"
     CDS             complement(4500..>4835)
                     /locus_tag="ACHUN9_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0697166.1"
                     /translation="TEDIIYRIVASSVCNRWGDPDIGGLIIAAYQGEADGDKVIRLVR
                     GQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYSALSGVPDAHVSGA
                     MPGLFIRTS"
BASE COUNT         1124 a         1230 c         1182 g         1299 t
ORIGIN      
        1 tacgataacc cactcttttt ttgccccttt tttgcccctt ctacagcgtt ttgccccatt
       61 tttgccatca acaattttcc tcataaagtc atttcaaagc ctaatagctg ctctactttt
      121 ttcctcaata tcatgtcgcc agcaggaaac aacctaatcc ctgttttagg cctcaaagct
      181 gttaatgata atcattttca ttttgtatgg gttatgctat aaaacatagc cgttgctatg
      241 tttgttctaa ttttgctcac tataggtact aaattatgca ctcaataaaa aaagtaacca
      301 tgctcttggg gtgcctcgcc ctcacctgct cgatcgcatt tcaggcaagt gcaactgaaa
      361 aattcaaggt cattacaaca tttaccatca tcgcggatat ggccaaaaac gtggctggag
      421 atgctgcaga agtctcatcc ataaccaagc ctggtgcaga aattcatgag tatcagccta
      481 cccctggcga tattaaacgt gcgcaggggg cacaactgat tctcgccaat ggtatgaatc
      541 tggaattgtg gttccaacgc ttttaccagc atctcaatgg ggttccagaa gtaattgtct
      601 cttcgggtgt gacgccagta gggatcaccg aaggacccta tgagggcaaa cctaatcccc
      661 atgcatggat gtcgccagat aatgctctga tttacgtcga taatattcgt gatgcgttga
      721 taaaatacga tccggcaaat gcacaaacct accaacgtaa tgccgatact tataaagcca
      781 agattaccca aacccttgcc cccctgcgta agcagattac ggaactccct gagaatcagc
      841 gatggatggt caccagtgaa ggggcttttt cttatctcgc acgcgatttg gggctaaaag
      901 agctttatct gtggccgatt aatgccgatc aacaaggaac accgcagcag gtacgtaagg
      961 ttgttgatat agttaagaaa aatcatatcc cggcagtctt tagcgagagt acgatttccg
     1021 ataaaccagc gcgtcaggtt gcgcgtgaaa ccggcgcaca ctacggcggt gtgctctatg
     1081 tcgattccct gagcacagaa aacggcccgg tacctacgta tatcgacctt cttaaggtta
     1141 ctaccagtac gctggtacag ggaattaaag ccgggaaaag ggagaaataa tgatgcaatc
     1201 tgcaggcatt gtcgttaatg atgttactgt cacctggcgt aacgggcaca cagcactgcg
     1261 cgatgcatcc ttcacagtac caagtggatc tattgccgct ctggttgggg taaacggttc
     1321 cggaaaatcg acgttgttta aagcgattat gggatttgtg cgtctgacca gcggaaagat
     1381 atctgttctg ggtattccca cgcgacaggc gctacagaaa aacctggttg cctacgtccc
     1441 tcagtcagaa gaggttgact ggtcatttcc tgtactggtg gaagatgtgg tgatgatggg
     1501 ccgctatggg catatgggta tgttgcgtat agccaaaaag agggatcgtc agattgtcac
     1561 cgatgcgctg gaacgtgtcg atatggtgga ttttcgccat cgtcaaatcg gcgagctttc
     1621 cggtggacaa aagaaacgcg tctttctggc gagagcgatt gcacagcagg gtgatgtgat
     1681 cctgctggat gaacctttta ccggcgttga tgtgaaaacc gaagcgaaaa tcatcagcct
     1741 gttgagggag ttacgcgcgg aaggcaaaac gatgcttgtc tcaacgcaca atctcggctc
     1801 agtcacgaca ttttgcgatt atacggtcat ggtcaaaggc accgtcctgg caagcgggcc
     1861 gacagacacc acttttacag ccgagaacct ggagctggct tttagcggcg tactgcgcca
     1921 tgtcacactt aacgactcgg aagaaagcat tattactgac gatgaacgcc cttttgtggc
     1981 acatcgaccg tcagcggtgc agagggaagc aagatgaacg tgctactgga acccttcagc
     2041 tatgaatata tgctcaatgc gatgtgggtc tcggcaatgg tcggcggtct ctgcgctttc
     2101 ctgtcatgct atctgatgct aaaaggctgg tcgctgattg gcgatgcgct gtcgcactct
     2161 attgtcccgg gtgttgcggg ggcatatatg ctggggcttc cgttttctct gggagccttc
     2221 ttttccggcg gactggcagc gggcagcatg ctgtttctta accaacgcac ccgcttaaaa
     2281 gaagatgcca tcatcggcct gatcttctcg tccttttttg gcctggggct gtttatggtg
     2341 tcattgaacc cgacatccgt gaacattcag accatcgtcc tgggcaatat tctggcaatc
     2401 gatccggcgg atatcctgca actcactatc attggcattc tctcgatcat cgtgcttttt
     2461 ttcaaatgga aagatcttat ggtgaccttt ttcgacgaga atcacgcccg tgctattgga
     2521 ttacatccgg gcagattaaa aatcctcttc ttcaccctgc tgtcggtttc gaccgttgcg
     2581 gcgctgcaaa cggttggtgc ctttctggtg atctgtctgg tggtgactcc cggtgcaaca
     2641 gcatggttgt taaccgatcg ttttccccgc ctgctcataa ttgctgtaac tattggcagt
     2701 gtaaccagtt tcctcggcgc gtgggtcagt tactttctgg acggcgcgac aggtggaatc
     2761 atcgtggtgg cgcagacact gctgtttctt ttagcatttg tatttgcgcc cacccatgga
     2821 cttttggcaa atcgtcggcg tgcgcataaa gcgctggagg atcgctcatg atggcattat
     2881 tactggagcc actgcagttc acctttatga gtcacgcact gctgatttca ctggtagttt
     2941 ccataccctg tgcgctgctg tcggtctttc tggtcctgaa aggttgggca ctgatgggtg
     3001 acgcgatgag tcatgcggtt tttcctggca tcgtgcttac ctggattctg gggttacctc
     3061 tggctacagg ggcattcgtc gccggagtat tctgcgcggt ggcgacggga tacctgaaag
     3121 acaacagccg aatcaagcaa gatacagtta tggggattgt tttttctggc atgtttgccg
     3181 caggtctgat cttgtatatt gcaatcaaac cagacgtaca tcttgaccat attcttttcg
     3241 gcgacatgct cgggataacc atcggcgata taatccagac ggtgattatt gccgggctgg
     3301 ttacacttgt tattagcgca aaatggcggg attttttgct gttcagcttt gattatcaac
     3361 aggcgcaggt aagcggtttg catacgagat ggctgcatta tgggctgctg tgcatggttt
     3421 ccctgaccat tgtggcgacg ctgaaagcag taggtatcat tctctctatt tctctgctca
     3481 ttgcgcctgg cgccattgca gtactcctga cacaacgctt tcatattgcg ctcctactgg
     3541 cgactggcat atcggtaata gtgtcaatga ctggtgtctg gctgtccttt tttatcgaca
     3601 gcgcaccagc tccgacgatt gtcgtcttat tcgcggtcgt gttcatcatg acgtttaccg
     3661 ttaccagcat caacgcacgc acaaagggaa acgcccatac acaggatctg ttatcaccca
     3721 attagcacaa catccgagga cagcatcgtc cgccagccta cagaagtccg gaaagccatg
     3781 gaaggtcgtt gggaggaggt acgaattcta tcatgcaaaa aatacgtaaa atcgataacg
     3841 cctagaaatc attcaatact cgcactatcg aaagtttacc agccaaccgc ggcacgttcc
     3901 tgcatacgac gtgccgcagc ttctgttatt ccacgataaa attcctaacc gaattcaaat
     3961 ccttcagact attcagctct tccttcagct cccgctgacg gcgataaatc tcgtcattgc
     4021 gctcaacctg tgcctgagcc attgctgccg ccagttcttc cagttccggc atcgacagtt
     4081 tcacctgctg attatcggca tcaccccacg tcatcacgtc ccgtacaatg tcggatttcg
     4141 cagccattac caccggataa aggcggccca gtgagttggg gccagcattc cagatacgac
     4201 cgttccattc aaacgtgaac ggcttcgcct cctgttctgt gcgccatgct tcaattccct
     4261 gacgtctggc ctctctggcc gcttccagca tttctggtgt cacagtgaat ggggctatct
     4321 caccccattt gccactttgc agttcctgcc agattcgctg acccgtcggt gcgacatcat
     4381 cagcggtggc tgtgtagggg actgcctggt ccctgtcgtc aaaaaaaacg tcacagtcta
     4441 ctgcgccact ttcggtataa cggggattaa tgattttttt aatttccacg gtgcattcct
     4501 cacgatgtgc gaataaaaag cccgggcatt gcgccagaga catgagcatc cggcaccccg
     4561 gacagggcgc tatatgaccc cggtaatgaa tgctctgaac atcccgtaat gaaaaattgt
     4621 ggggatgcta tatacgttcc ggtgggagta ctgggcactg aaatccccac cggtcccagt
     4681 cgtgagcctc tgtatgactg ccccctgaca agtctgatga ctttatcacc gtcagcttct
     4741 ccctggtacg cagcaataat cagcccgcca atgtcagggt ctccccatct gttgcagaca
     4801 gagctcgcca cgattctgta aataatatct tctgt
//
LOCUS       DAZOYS010000250         4834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOYS010000250 DAZOYS010000000
VERSION     DAZOYS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.252"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHUN9_003892"
     CDS             complement(108..551)
                     /locus_tag="ACHUN9_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0697167.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHUN9_003893"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHUN9_003893"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0697168.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUN9_003894"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUN9_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUN9_003895"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUN9_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1205 a         1272 c         1225 g         1132 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta tatcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aacc
//
LOCUS       DAZOYS010000251         4821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOYS010000251 DAZOYS010000000
VERSION     DAZOYS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.253"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            131..1105
                     /gene="hemB"
                     /locus_tag="ACHUN9_003896"
     CDS             131..1105
                     /gene="hemB"
                     /locus_tag="ACHUN9_003896"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0697169.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1210..2061)
                     /gene="tauD"
                     /locus_tag="ACHUN9_003897"
     CDS             complement(1210..2061)
                     /gene="tauD"
                     /locus_tag="ACHUN9_003897"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0697170.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2058..2885)
                     /gene="tauC"
                     /locus_tag="ACHUN9_003898"
     CDS             complement(2058..2885)
                     /gene="tauC"
                     /locus_tag="ACHUN9_003898"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0697171.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2882..3649)
                     /gene="tauB"
                     /locus_tag="ACHUN9_003899"
     CDS             complement(2882..3649)
                     /gene="tauB"
                     /locus_tag="ACHUN9_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0697172.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3662..4624)
                     /gene="tauA"
                     /locus_tag="ACHUN9_003900"
     CDS             complement(3662..4624)
                     /gene="tauA"
                     /locus_tag="ACHUN9_003900"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0697173.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1109 a         1401 c         1232 g         1079 t
ORIGIN      
        1 tttctattac aaagatgtcc tggtctctct ttcattctct tcttgtcaac atcgcgacaa
       61 ctttcgtaaa acactcctgc cccgcttcag gtatactgtg caccttaatt ccacaaacat
      121 caggcagacc atgacagact taatccaacg ccctcgtcgc ctacgcaaat ctcctgcgct
      181 gcgcgctatg tttgaagaga caacacttag ccttaacgac ctggtgttgc cgatctttgt
      241 tgaagaagaa attgacgact acaaagccgt tgaagccatg ccaggcgtga tgcgcattcc
      301 agagaaacat ctggcacgcg aaattgaacg catcgccaac gccggtatcc gttccgtaat
      361 gacttttggc atctctcacc ataccgatga aaccggcagc gacgcctggc gggaagatgg
      421 actggtggcg cgtatgtcgc gcatctgcaa gcagactgtg ccagaaatga tcgtcatgtc
      481 agacacttgt ttctgtgaat acacttctca cggtcactgc ggtgtgctgt gcgagcatgg
      541 cgtcgacaac gacgcgactc tggaaaattt aggcaagcaa gccgtggttg cagctgctgc
      601 aggcgcagac ttcatcgccc cttccgccgc gatggacggc caggtacagg cgattcgtca
      661 ggcgctggac gctgcaggct ttaaagatac ggcgattatg tcgtattcga ccaagttcgc
      721 ctcttccttt tatggcccgt tccgtgaagc tgccggaagc gcattaaaag gcgaccgcaa
      781 aagctatcag atgaacccaa tgaaccgtcg cgaggcgatt cgtgaatcac tgctggatga
      841 agcccagggc gcagactgcc tgatggttaa acctgctgga gcgtacctcg acatcgtgcg
      901 tgagctgcgt gaacgtactg aattgccgat tggcgcgtat caggtgagcg gtgagtacgc
      961 gatgattaag ttcgccgcgc tggcgggtgc tatagatgaa gagaaagtcg tgctcgaaag
     1021 cttaggttca attaagcgtg cgggtgcgga tctgattttc agctactttg cgctggattt
     1081 ggctgagaag aagattctgc gttaattttt ttcctctcag aaagggggag ttaacctgtt
     1141 gaaagtacgg tgaacgcccc ctcaccctaa ccctctcccc aaggggcgag ggaaccgtcc
     1201 gcactcgtat caccccgccc gataaaacgg tttatcccca aggatcgtcg cccgatgcat
     1261 aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca
     1321 aatcgcaata tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg
     1381 ggcgaataaa aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt
     1441 agtaaagcct tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac
     1501 cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc
     1561 ggttttgcgg tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc
     1621 gctcagcagc tggcggaagg gaacagagag cgcctcatag gccgcaatac cgctggtcca
     1681 gagcgtatca ccgccggtcg aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg
     1741 cgtttcaata aatgtcacat cggtatgcca gttgtcgtta tccggcggat tatcgttatg
     1801 ggtatccagc acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg
     1861 cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg
     1921 cagaaatacc acctgatagc gcagcaccgc atggtaaagc tgttcaaact gattatcgct
     1981 taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt
     2041 aatactcaga cgttcactca ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac
     2101 gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt
     2161 accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgtgtc
     2221 gcggcaatca gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat
     2281 ccggtgagga tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg
     2341 ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc
     2401 agcaccgaca tcgccaccgg tgcaaaaatc gctaaataga tcaataagat cttcgaggtt
     2461 tcaccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga
     2521 taaagttcga ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc
     2581 ccgaccggaa taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg
     2641 gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc
     2701 agttttgcca gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc
     2761 gccaccgtcc accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga
     2821 cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca
     2881 ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga
     2941 tgaattgtgg atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa
     3001 agttgagcgg cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag
     3061 tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct
     3121 cctgccagag tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac
     3181 caaacggttc gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca
     3241 cccgctgacg ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc
     3301 ccactttttt cagcatctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca
     3361 attgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtagtagc ccttcattct
     3421 gaaaaaccac cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc
     3481 tgccatgctg ataaggcaca aaaccggcaa tcagattcag cagggtggtt ttaccgcagc
     3541 cggacggccc cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca
     3601 gtgccggttt gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct
     3661 tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct
     3721 tgccctgctc tttcaaaaac tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca
     3781 gttctgccgt ttgttgctgc ggcgtcagat aggtattccc cttcaccagc cccggaacgt
     3841 caccttccgg cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc
     3901 acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt
     3961 tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg
     4021 gcgcacccca ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg
     4081 cgttaaccgc cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga
     4141 taatcgcggg cggctgcagg ttcacaatct ccacttgccc tggtttaatc ccccagtgtt
     4201 tcagcgccgc cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc
     4261 caatcaaatc ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca
     4321 gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg
     4381 ggctggaacc gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg
     4441 ctccgctgtc aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgt
     4501 cggcctgagc cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg
     4561 cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg
     4621 ccatgattgt ctgctcccct gtcttgttat gggagcagta ttcaggaata aaaacattca
     4681 ttaaaagaat tagtcgttat cgcacagatg attttattct tagcaaaaaa acggcaaagg
     4741 aaagattaaa tatgcaatta ctccgttcta acaattggga aaattactac acttcatttt
     4801 agtgatgcat aaacaaccac a
//
LOCUS       DAZOYS010000252         4820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOYS010000252 DAZOYS010000000
VERSION     DAZOYS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.254"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHUN9_003901"
     CDS             complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHUN9_003901"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0697174.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHUN9_003902"
     CDS             complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHUN9_003902"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0697175.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2005..3186)
                     /locus_tag="ACHUN9_003903"
     CDS             complement(2005..3186)
                     /locus_tag="ACHUN9_003903"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0697176.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3441..4670
                     /locus_tag="ACHUN9_003904"
     CDS             3441..4670
                     /locus_tag="ACHUN9_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0697177.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1256 a         1223 c         1057 g         1284 t
ORIGIN      
        1 tacaggtata aaaaaccctg ccatgaggca gggtcataaa agtaagagga atgaattaac
       61 gagccagcca accgccatct acggcaatgg tataaccatt cacataatct gaagcgctgg
      121 aggcaaggaa cactatcggc cccatcaggt cactcggcag tccccaacga ccagctggaa
      181 tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga gtattgttgg
      241 tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc cattcgttcg
      301 ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa ggcacacgga
      361 tcccgccctg gaaggagagc attgacgtga tattgataat cttgccgcca ttgcgttgcg
      421 cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata ttcaggttca
      481 tgacatcgtc ccagtctttt tcgctgaact cgagagcatc ttcgcggcga atcaatccgg
      541 cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc agcagtgctg
      601 gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg
      661 tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg tcacagcccg
      721 cttgcgccag ccccaacgcc atcccctggc ccagtccagt atcacaacca gtgacgaccg
      781 caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat tcgtttatgc
      841 cgacaactag cgcaaatctt taacggccac tatgccgaca actagcgcaa atctttaacg
      901 gccacatggt ccatatcatc aaagacctgg ttttcaccaa ccatgcccca gataaaggta
      961 taagctttgg ttccgacgcc ggaatggatc gaccagctgg gggagatcac cgcctgctcg
     1021 ttatgcatca caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg
     1081 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgagt gtgacacggc
     1141 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttggcaggtt
     1201 tccagcacat ccgggacaaa atatttgtta attgtgcgac ggttactggt gaggttatcg
     1261 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
     1321 gcgggtgcgc aattgtaata aaactttgcc ggagtggcgg tatcaatact ggcaaagaca
     1381 acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattgg
     1441 ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca
     1501 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
     1561 atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca
     1621 tcggcgacaa ataccttttc aaccaaaaat tcattgcgca gcccttgggt atccagcgtt
     1681 tttgcgtgcg cactgtggat gccctgtctt acgtccacat caacctccga taatcagtga
     1741 agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata
     1801 tgcccatggg ctagggtttt caattaaaaa taaaacaatg tttcattttt aagttaagga
     1861 ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc
     1921 agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt
     1981 tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgccctg
     2041 gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg
     2101 aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc
     2161 gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc
     2221 ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac
     2281 acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac
     2341 accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt
     2401 ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc
     2461 ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc
     2521 gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat
     2581 taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg
     2641 cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt
     2701 ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag
     2761 actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg
     2821 tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg
     2881 tatcgcctga gtagccagat gcagtgcttt aagtccggaa ccgcaaacgt cattaatcgt
     2941 gattgcagaa acgctattag gaagaccacc tttaatagcc gattgccttg ccggattctg
     3001 ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac
     3061 gccggtacgt tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc
     3121 cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc
     3181 tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta
     3241 tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca
     3301 gagtcataac tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagataag
     3361 gcgttaatct cccatactta acctggttta tggtaaattg ccctccattt tgtttaattt
     3421 gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt
     3481 tcgcgctcta cggcacagcc gttggtgcag gcacgctctt ccttcctatt cagttaggtt
     3541 cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattggc
     3601 cacataaagc cttatgccag ttcatcctct catcaaaaac atcagcatgt gaagggataa
     3661 cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact
     3721 tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg
     3781 aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg
     3841 ttgtattaat tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg
     3901 gatttttggt attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta
     3961 gttggcaacc tgatctatta acgacccagg tagagttcaa tcagaatacc cttcaccaga
     4021 tatggatatc gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt
     4081 ttgccataga cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta
     4141 tgaaagtcgc ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc
     4201 tttctattcc accttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg
     4261 cgctttctat gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag
     4321 tagttgcgat gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacgg
     4381 aggtcgtcaa aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccgcg
     4441 cactatcaat tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg
     4501 cgatttcgat gatttatgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc
     4561 ctacgttgtc aacgtatctg atcccggcgc ttaaaccctg gcgctccatc ggaaatctga
     4621 ttacgctgat cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt
     4681 ggatggttca aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta
     4741 ttattccgcc ctatcacttc tctcgtcata tcctagaatt atcagttagt taaacacaca
     4801 aagagatctg aacatcttaa
//
LOCUS       DAZOYS010000253         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOYS010000253 DAZOYS010000000
VERSION     DAZOYS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.255"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUN9_003905"
     tRNA            6..81
                     /locus_tag="ACHUN9_003905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUN9_003906"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUN9_003906"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUN9_003907"
     CDS             complement(299..1057)
                     /locus_tag="ACHUN9_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0697178.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUN9_003908"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUN9_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0697179.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUN9_003909"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUN9_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHUN9_003910"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHUN9_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZOYS010000254         4706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOYS010000254 DAZOYS010000000
VERSION     DAZOYS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.256"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUN9_003911"
     CDS             complement(<1..737)
                     /locus_tag="ACHUN9_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0697180.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUN9_003912"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUN9_003912"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0697181.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUN9_003913"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUN9_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0697182.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUN9_003914"
     CDS             4397..4678
                     /locus_tag="ACHUN9_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0697183.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1131 a         1171 c         1182 g         1222 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg cattta
//
LOCUS       DAZOYS010000255         4554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOYS010000255 DAZOYS010000000
VERSION     DAZOYS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.257"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUN9_003915"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUN9_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUN9_003916"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUN9_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0697184.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUN9_003917"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUN9_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0697185.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUN9_003918"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUN9_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0697186.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUN9_003919"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUN9_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0697187.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUN9_003920"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUN9_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0697188.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          984 a         1347 c         1145 g         1078 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgctgggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttca
//
LOCUS       DAZOYS010000256         4483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOYS010000256 DAZOYS010000000
VERSION     DAZOYS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.258"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHUN9_003921"
     CDS             complement(8..1069)
                     /locus_tag="ACHUN9_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0697189.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHUN9_003922"
     CDS             complement(1066..2463)
                     /locus_tag="ACHUN9_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0697190.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHUN9_003923"
     CDS             2618..3616
                     /locus_tag="ACHUN9_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0697191.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUN9_003924"
     CDS             complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHUN9_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0697192.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gcc
//
LOCUS       DAZOYS010000257         4442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOYS010000257 DAZOYS010000000
VERSION     DAZOYS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.259"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(39..3143)
                     /gene="acrF"
                     /locus_tag="ACHUN9_003925"
                     /pseudo
     CDS             complement(39..3143)
                     /gene="acrF"
                     /locus_tag="ACHUN9_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3155..4312)
                     /gene="acrE"
                     /locus_tag="ACHUN9_003926"
     CDS             complement(3155..4312)
                     /gene="acrE"
                     /locus_tag="ACHUN9_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0697193.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1104 a         1179 c         1085 g         1074 t
ORIGIN      
        1 ataataaagg cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg
       61 atcaccacaa agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct
      121 cccattaccc cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct
      181 agcggtaata cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata
      241 cgtactgcca tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta
      301 gcgaactcaa caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc
      361 accataaagt agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca
      421 atccctaacg gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca
      481 gcaaggcaca ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc
      541 gataagcgtt cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt
      601 gacgcaaggt tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc
      661 ccctgaatct ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc
      721 caatgtgaag tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa
      781 agtttatcga catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt
      841 ttcacgcgac cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg
      901 gtctgattga tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc
      961 agtttaaact gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga
     1021 tgttgcgccg ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc
     1081 ccagcctgat caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct
     1141 ggcatattga atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga
     1201 tggattaccg cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg
     1261 acgaaagcca taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag
     1321 acactttcta cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact
     1381 ttttgcgtcc gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc
     1441 tgatcctctt caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca
     1501 acaatcagcg catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg
     1561 ttggtgtagt ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc
     1621 ttattttcgt gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta
     1681 agaatcaatg ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg
     1741 cgataaattg ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc
     1801 accatcgcga tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct
     1861 tttggcggga gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca
     1921 tcatcgacga gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag
     1981 taaccaaaag cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg
     2041 gggatcagcg ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata
     2101 atggcttcga acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg
     2161 tcataagggt agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt
     2221 gccttaatgg ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg
     2281 cccgccgccg gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca
     2341 actcgtgcga catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact
     2401 ttgccgaatt cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt
     2461 tgccctggta acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc
     2521 ttcaactggt taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc
     2581 agccagatac gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga
     2641 cggctaagcg tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg
     2701 tctgggttat cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca
     2761 ctgatcccct gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc
     2821 tgcacctgca cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc
     2881 acgctaccgg cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata
     2941 ttctgttcga taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag
     3001 tttgcctaaa cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt
     3061 aggatcgcca gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt
     3121 cgtcgaataa aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg
     3181 tatcggtagt ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga
     3241 cttgatcgcc agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg
     3301 caacgacagg gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac
     3361 cacgcggtgt gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt
     3421 catcaatccg tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga
     3481 cagcacgtag ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg
     3541 tacctttcag gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt
     3601 tttccttatg caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg
     3661 attgggtcac atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt
     3721 gcccattagt gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg
     3781 gcgcagtcac tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg
     3841 caatcacggc ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc
     3901 tgatgtattt cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg
     3961 cggcggcttc acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg
     4021 cgggatcgat ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac
     4081 gattcagtac gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc
     4141 ggcctggtaa ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct
     4201 gcggttcacc gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg
     4261 cggagatcag aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc
     4321 ctcaaaaaaa caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga
     4381 aaaatacagt ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata
     4441 ct
//
LOCUS       DAZOYS010000258         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOYS010000258 DAZOYS010000000
VERSION     DAZOYS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.260"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUN9_003927"
     CDS             complement(<1..105)
                     /locus_tag="ACHUN9_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697194.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUN9_003928"
     CDS             complement(173..2122)
                     /locus_tag="ACHUN9_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0697195.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUN9_003929"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUN9_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0697196.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUN9_003930"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUN9_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0697197.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUN9_003931"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUN9_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0697198.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATSRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          971 g         1104 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgagatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZOYS010000259         4396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOYS010000259 DAZOYS010000000
VERSION     DAZOYS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.261"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUN9_003932"
     CDS             complement(<1..590)
                     /locus_tag="ACHUN9_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0697199.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUN9_003933"
     CDS             complement(583..993)
                     /locus_tag="ACHUN9_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0697200.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUN9_003934"
     CDS             complement(999..1532)
                     /locus_tag="ACHUN9_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0697201.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHUN9_003935"
     CDS             complement(1510..2586)
                     /locus_tag="ACHUN9_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0697202.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="ACHUN9_003936"
     CDS             complement(2583..3974)
                     /locus_tag="ACHUN9_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0697203.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..>4396)
                     /locus_tag="ACHUN9_003937"
     CDS             complement(4021..>4396)
                     /locus_tag="ACHUN9_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697204.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgtt
//
LOCUS       DAZOYS010000260         4385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOYS010000260 DAZOYS010000000
VERSION     DAZOYS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.262"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHUN9_003938"
     CDS             complement(<1..3511)
                     /locus_tag="ACHUN9_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0697205.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4385)
                     /locus_tag="ACHUN9_003939"
     CDS             complement(3963..>4385)
                     /locus_tag="ACHUN9_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0697206.1"
                     /translation="ESANYIGARSVTAAIKPDIKLQPTRHVKTMDALQTGCVHINTVN
                     KALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKA
                     EHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1124 a         1126 c          906 g         1229 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 gtatttatat gaacgcatcc cgtttgcaag gcatccattg ttttgacatg acgggtaggt
     4321 tgcagcttta tatccggctt tattgcagcc gtaacgctgc gagcccctat gtaattcgcc
     4381 gactc
//
LOCUS       DAZOYS010000261         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOYS010000261 DAZOYS010000000
VERSION     DAZOYS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.263"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUN9_003940"
     CDS             complement(<1..215)
                     /locus_tag="ACHUN9_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0697207.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUN9_003941"
     tRNA            complement(330..405)
                     /locus_tag="ACHUN9_003941"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUN9_003942"
     tRNA            complement(412..486)
                     /locus_tag="ACHUN9_003942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUN9_003943"
     tRNA            complement(603..687)
                     /locus_tag="ACHUN9_003943"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUN9_003944"
     tRNA            complement(696..771)
                     /locus_tag="ACHUN9_003944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUN9_003945"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUN9_003945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0697208.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUN9_003946"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUN9_003946"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0697209.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUN9_003947"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUN9_003947"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0697210.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUN9_003948"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUN9_003948"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZOYS010000262         4329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOYS010000262 DAZOYS010000000
VERSION     DAZOYS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.264"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..582)
                     /locus_tag="ACHUN9_003949"
     CDS             complement(<1..582)
                     /locus_tag="ACHUN9_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0697211.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(645..1094)
                     /locus_tag="ACHUN9_003950"
     CDS             complement(645..1094)
                     /locus_tag="ACHUN9_003950"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0697212.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHUN9_003951"
                     /pseudo
     CDS             complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHUN9_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1043 t
ORIGIN      
        1 gaagttcggg atcatcgcgt caacggtgat ggcgtcagca ggctgtacgc cgccaaccag
       61 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
      121 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
      181 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt atccgaggaa
      241 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
      301 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
      361 tgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
      421 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
      481 atcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
      541 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
      601 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
      661 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
      721 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
      781 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
      841 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
      901 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
      961 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
     1021 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
     1081 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
     1141 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
     1201 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
     1261 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
     1321 gcagctcgtt acagtgctgg gcagcgtgga tgttttccta ggtatagtgc caggcgctga
     1381 agttaattgg gcgctgcgga accaccagca gaccgttacc gtggcggtta gtcagtgccg
     1441 tccagcggac atgctgacgg ttaccgttgt tctgcgggaa gggatagttc tcgaacatgg
     1501 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
     1561 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatt cccatggtga
     1621 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
     1681 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcattccg aaatcaaaca
     1741 ccggcggggc aataaccgtg cggctgatga tcaacacttc accctcgctc tgttctacgg
     1801 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
     1861 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
     1921 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
     1981 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
     2041 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
     2101 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
     2161 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
     2221 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
     2281 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
     2341 tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac cggcgcgata acctgtttgt
     2401 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
     2461 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgtggt
     2521 cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc tgaatgcaat
     2581 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
     2641 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
     2701 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
     2761 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
     2821 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
     2881 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
     2941 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
     3001 tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
     3061 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
     3121 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
     3181 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
     3241 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
     3301 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
     3361 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
     3421 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
     3481 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacgg
     3541 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
     3601 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
     3661 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
     3721 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
     3781 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
     3841 gttgataggc accggttggg ttatcgctgg gaacaaacgg cacatcgatg gggaacggaa
     3901 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
     3961 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcgggt
     4021 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc
     4081 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
     4141 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
     4201 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
     4261 tttttactaa aaagtggcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
     4321 cagcactgt
//
LOCUS       DAZOYS010000263         4312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOYS010000263 DAZOYS010000000
VERSION     DAZOYS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.265"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHUN9_003952"
     CDS             complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHUN9_003952"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0697213.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     "
     gene            complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHUN9_003953"
     CDS             complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHUN9_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0697214.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2272..2979
                     /locus_tag="ACHUN9_003954"
     CDS             2272..2979
                     /locus_tag="ACHUN9_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0697215.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3085..3160
                     /locus_tag="ACHUN9_003955"
     tRNA            3085..3160
                     /locus_tag="ACHUN9_003955"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3118..3120,aa:Phe,seq:gaa)
     gene            3358..>4312
                     /locus_tag="ACHUN9_003956"
     CDS             3358..>4312
                     /locus_tag="ACHUN9_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0697216.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1115 a         1141 c         1037 g         1019 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcagggtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaattgcgc cagcatcgcc accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acacagatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc cccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cgacgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatccttc gccgatcctt
     1381 catgaataag cagatcgccc aattttacgt cagcgttaca catatcggcg cgaaagacgt
     1441 tctcaccaaa gaaatcgtaa aaatggcgtc cggcaggatg ctttttaaaa aacgcaccat
     1501 gttgatgtcc agggcaagca aaggtgctgt tgcccatctc aacgtactgc gtcagcgtgt
     1561 cataaaacgg tggcagcaaa ttctcttcat actgacaggc tgcggattcc agctccagcc
     1621 actgctgctc gttgccgttg attaccgccg taacgcccgc aggtaattca acagcatgtt
     1681 cggaatacaa aaacaccggt agatgaaaac cggtgcgctt aagcaacgca agaatgccac
     1741 tgcgactatc cgcagcggta atgacgactg ccgcgacatc cgtaaaatca gtatctccca
     1801 acgccaccac gcggcgatga gtagaaagtc gggataccag ttcactactg gcggcaatat
     1861 tcattgattt cataagcgca aaacccgttt cggggaagta agaataccgg acaaagtgta
     1921 aaaccctgcc ccatgagata tgggtcaaac tggtcaccag ctccgaccgc cagacatcag
     1981 taaaagcaga aacgctctga ttttactgtt gtcctgcagt gaacgtgcgt taacttcacc
     2041 gcatgagcag taacatagaa agggaaacgt ttcgcgcgaa acggcgataa gcgagagaat
     2101 gtaaggagat ggcgtgcatc gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca
     2161 tggcaccttt cgctaaggcg tgcaagccag gactttgcga ccagacaagc catcaacaag
     2221 gcaaaaagtt cataataaga aaatcaaaca atacagctaa caggaactct tgtggtcatc
     2281 ggccctttta tcaacgctag tgcagtctta ctgggtggcg ttctcggcgc actgctcagc
     2341 caacgcttac cggaacgtat ccgcgtctcc atgacatcta tttttggtct ggcatcgctg
     2401 gggattggta ttttactggt tgtgaaatgc gccaaccttc cagcgatggt tttagctacg
     2461 ctacttggcg ctctaattgg cgaaatttgt ttgctggaga aaggtgtcaa tacagcagtc
     2521 gccaaagcac aaaatctgtt tcgtcactca cgtaagaagc cagcgcatga atcttttatt
     2581 cagaattatg tcgcgattat tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg
     2641 atgaacgaag ggatgaccgg cgatccgagt attttaatcg ccaagtcatt tcttgatttc
     2701 tttacggcga tgatcttcgc ctgctcgctg ggtattgcgg tgtcggtaat tagtatccca
     2761 ttactgatca tccagctaac gctagcgtgg gctgccgcgc tgatattacc gctgaccaca
     2821 ccgtcgatga tggcagactt cagcgccgta ggcggtttat tgctgctggc aaccggatta
     2881 cgcatctgtg gcattaaaat gttcccggtg gtcaacatgc ttccagcact ccttctggca
     2941 atgccgcttt ccgccgcctg gaccgcctgg tttgcctgac aatgcgtgca atagcggcaa
     3001 agtgatgata gattgtgcag tctgcagtaa attgaagaaa tttgattgac gagacgaggc
     3061 gaatcaggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa
     3121 aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc
     3181 cacggaggtc cgtttttcgt ttcagaacgc cacgatttaa gcgttctgcc tacaaatcaa
     3241 ttctaccgaa ctcaaccaga ttccccccac atcaacccca ttgtgtgggt ataattgcgg
     3301 gcatacccca gttcgacaga atttgtaccc tctttaacgc attcagaagg ctgaactatg
     3361 gcactgactg acgcaaaaat ccgggctgca aagcccactg acaaggctta taaactcact
     3421 gacggggctg gcatgttcct gctggtacat cctaatggct cccgttactg gcgtctccgt
     3481 tatcgtattc tgggtaagga gaagactctg gcacttggtg tgtatccaga agtttctctc
     3541 tccgaagctc gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg ggttgaccct
     3601 tgcgaacaga aaagagctaa aaaagtagtc cctgatttac agctctcttt tgaacatatt
     3661 gcgcgacgct ggcatgccag taataaacaa tgggcacaat cacacagcaa taaagtactc
     3721 aaaagccttg agactcacgt tttccccttt atcggcaacc gggatatcac aacactcaat
     3781 accccggatc tgcttatccc tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc
     3841 agtcgtctgc agcaaagaat atctgccgta atgcgttatg ccgtacagtc tggcatcatc
     3901 agatataatc ctgctctgga tatggctggc gcattgacta cggtaaaacg ccagcatcgc
     3961 cccgctcttg atctttctcg cctgcctgaa cttttgtcgc gtattggcag ttacaaaggg
     4021 caacctgtca cccagcttgc cgttatgctg aatttactgg tttttattcg ttccagtgaa
     4081 ctcagatacg cccgctggtc tgaaattgat attgacaatg ccatgtggac tattccagcc
     4141 gaacgcgaac ccctgccagg cgtaaaattc tcacaccggg gctccaagat gcgaacacca
     4201 catcttgtgc cactcagcaa acaggctgta gccatactga cagaactaca gacatgggct
     4261 ggtgaaaatg gtctgatctt tacgggagca catgacccgc gtaaaccaat ca
//
LOCUS       DAZOYS010000264         4242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOYS010000264 DAZOYS010000000
VERSION     DAZOYS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.266"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..2417
                     /locus_tag="ACHUN9_003957"
     CDS             <1..2417
                     /locus_tag="ACHUN9_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697217.1"
                     /translation="AYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNALINNK
                     RTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYPIEKEKQITT
                     YLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNSQ
                     NISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIG
                     QYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQ
                     ENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMG
                     KPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSL
                     SFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYIND
                     TNHYMNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIG
                     PLKNVSASLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYD
                     SSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWN
                     GSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYIT
                     TSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLG
                     ISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVIL
                     PCISQN"
     gene            2433..3182
                     /locus_tag="ACHUN9_003958"
     CDS             2433..3182
                     /locus_tag="ACHUN9_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0697218.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3184..>4242
                     /locus_tag="ACHUN9_003959"
     CDS             3184..>4242
                     /locus_tag="ACHUN9_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697219.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1315 a          868 c          867 g         1192 t
ORIGIN      
        1 cagcttatgc cgttgaattc aacaaagatt taatcgaagc cgaagatcgt gaaaacgtta
       61 acctttccca atttgaaact gatggccaat tacccgtagg taaatactca ttaaacgctc
      121 ttattaataa taaaaggacg ccaatccatc tggacctgca atgggtatta attgataacc
      181 aaactgcagt ttgtctgact ccagagcaat taacattatt aggctttact gatgagatta
      241 ttgaagaggc tcagcaaaac ctgatcgatg gttgttaccc tatcgaaaaa gaaaaacaaa
      301 ttacaactta tctcgataaa gggaaaatgc aattatccat atctgcacct caggcatggt
      361 taaaatacaa agatgcaaac tggacgcctc ctgaactttg ggatcatggt attgctgggg
      421 catttcttga ctacaattta tatgcctctc attatgcacc acatcagggc gataattcac
      481 aaaatataag ttcctatggg caggctgggg ttaatcttgg ggcctggcgc ctgcgtactg
      541 attaccagta cgatcagtca tttaacaatg gcaaaagcca ggcgaacaac ctggattttc
      601 cgcgtattta tttgtttcgc ccaatcccag caattaatgc aaaactaact ataggtcaat
      661 acgatactga atcctctatt ttcgactctt tccatttttc tggcgtttcg ttgaaaagcg
      721 atgagaatat gttgccgcct gatctacgtg gttacgcacc gcaaatcacg ggtgttgcac
      781 aaacgaatgc aaaggtcact gtctcacaga acaaccgtat tatttatcaa gaaaatgttc
      841 ctcctggccc atttgctatt actaatttat tcaatacatt acaggggcaa ctggacgtca
      901 aggttgaaga agaggacggg caagtcacgc aatggcaagt tgcatctaat agtattcctt
      961 atctgacgcg taaagggcag attcgctaca ccactgctat gggtaaaccg accagcgttg
     1021 gtggtgattc cttacaacaa cccttcttct ggactggtga attctcatgg ggttggctga
     1081 acaatgtatc cctgtatggt ggttcagttt taacaaaccg tgattatcaa tctctggctg
     1141 ccggcgttgg ctttaacctt aactcattgg gttcattatc ttttgatgtc acacgatctg
     1201 atgctcagtt gcataatcag gataaagaaa cgggttatag ctaccgcgct aactattcaa
     1261 aacgttttga atctaccggt agccagctca ctttcgctgg ttaccgtttc tctgataaaa
     1321 actttgtgac aatgaatgaa tatatcaatg acactaacca ttacatgaat tatcagaatg
     1381 aaaaagagag ttatattgtc acgtttaacc agtatcttga atcattaagg ttaaatacat
     1441 acgtaagttt ggctcgtaat acttactggg acgccagcag taatgtgaat tattcattat
     1501 cacttagccg cgattttgat atcgggccat taaaaaacgt ctccgcttct ctaacattta
     1561 gccgaataaa ctgggaagaa gacaaccagg atcaactgta cctaaatatt tcgattccct
     1621 ggggaaccag tagaacattg agctatggta tgcaacgaaa tcaggataac aagatttcgc
     1681 atactgcttc gtggtatgac tcttccgatc gaaataattc ctggagcgtt tctgcttcag
     1741 gcgacaatga tgaattcaaa gatatgaaag cgtcactacg cgccagttat cagcataata
     1801 ccgagaacgg tcgtctctat ctctccggga catcacagcg agacagttat tattctctga
     1861 atgccagttg gaatggttca ttcacggcga ctcgtcacgg agccgctttc catgactata
     1921 gtggtagtgc tgactcgcgt tttatgatcg acgcagatgg tgcagaagat attccgttga
     1981 acaataaacg cgcggtaact aatcggtatg gcatcggagt tattccatca gtcagcagtt
     2041 acataacaac atcattaagc gttgataccc gaaatcttcc agaaaatgtg gatatcgaaa
     2101 actcggttat caccaccacc ttaaccgaag gtgctattgg ctacgccaaa cttgataccc
     2161 gcaagggcta tcaaatcatg ggggttattc gcctggcaga tggtagtcac ccaccactgg
     2221 ggattagcgt aaaagataaa accagccata aagaattagg actggttgct gatggcggct
     2281 ttgtatacct caacggcatt caggatgaca gcaaacttac tttacgctgg ggtgataaat
     2341 cctgttttat tcaaccaccc aatagcagca acttaaccac cggaacggtt attttaccgt
     2401 gtattagcca aaattaattc aggatgaaac taatgcgcta cttaaacaca aagaatctca
     2461 ttgcagcagg agtattactc gcctgcatga gcagcattgc ctggggtgcc atcatcccgg
     2521 accgtacccg tatcataatg aatgagtcag ataaagggga agcattaaaa cttactaatc
     2581 aaagcaaaaa tctaccctat ctggcgcaaa catggataga agatacgaaa ggcaataaat
     2641 cacgtgactt tattgtcacg gttcccccta tggtacgttt aaatcccagt gagcaaattc
     2701 aaataagaat gataagtcag gaaaaaattg ctcaactgcc taatgacaga gaaacgctct
     2761 tctattttaa cgtgcgagaa ataccgccaa aaacagataa aaaaaatgtg atgcaagtca
     2821 ctatgcagca cgcattgaaa ctattctggc gaccaaaagc tattgaatta gaagatgatg
     2881 gggtaatgtc ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg atccgcttta
     2941 acaataagat gccttatcac gttacgctgg gttatatcgg aacaaacgga atcacaatgt
     3001 tgccacaaac gcaaagttta atggtaaccc cttatagcca tgcagatacg cagtttaaaa
     3061 atgttccgtc agcttttcag gttggttata tcaatgattt tggtggatta agtttttatg
     3121 aaataaactg cccgacggtt aataactctt gtaatgtttc cgtagccaaa cgagataaat
     3181 aatatgcgct acttattaat tgtaattata tttattatag gttttagtgc attacctgta
     3241 tgggcaatgg actgctatgc tgaacatgaa gggggaaata ccgtagtcat tggttacgta
     3301 ccaagaattt ctatccccag taatggtaaa aaaggtgata agatctggca aagcagtgag
     3361 tattttatga atgtcttctg taataatgca ttacctgccc catcacctgg agaagaatac
     3421 ccatcagcct ggactaatat aatgatgttt ttaccaggcg gtcaggactt ttataatcag
     3481 aactcctata tttttggcgt aacatacaat ggagttgatt atgattcgac tgcccctaat
     3541 gctatcgcag caccagaatg cattgatata aaaggagcgg gtacctttaa taatcactat
     3601 aaaaaccccg ctgtctgtag tggaggtcct gagcctcaat tatcagtaac ctttccagct
     3661 cgcgtacaac tctatattaa attagcaaaa aatgccaata gagttaataa aaacctggtg
     3721 ttacctgatg aatatatcgc tctagagttt aaaggtatga gtggcgcagg agctatagaa
     3781 gtagacaaaa atttgacctt caggattcgt ggattaaata atattcatgt ccttgactgc
     3841 tttgttaatg tcgcactgga accagctgat ggcgttgttg actttggtaa aataaattcc
     3901 cgaacaatta aaaataccag cgttagtgag acgtttagtg tagtcatgac aaaagatcct
     3961 ggtgcggcct gtactgagca attcaatatt ttagggagct ttttcactac ggatattttg
     4021 agtgattata gccatctgga tatgggtaat ggactgctat tgaagatatt tcataaagat
     4081 ggaacagcaa cggaatttaa ccgcttctca caatttgctt ctttttcatc gtctagtgca
     4141 ccttcggtca ccgcaccatt cagggcagaa cttagtgcga acccggcaga gactgttgtt
     4201 gagggaccgt ttagtaaaga cgtaatcctc aaaatcacct at
//
LOCUS       DAZOYS010000265         4197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOYS010000265 DAZOYS010000000
VERSION     DAZOYS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.267"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..3276
                     /gene="evgS"
                     /locus_tag="ACHUN9_003960"
     CDS             <1..3276
                     /gene="evgS"
                     /locus_tag="ACHUN9_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0697220.1"
                     /translation="QKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVT
                     TLHDSMRPLTSSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDY
                     FIGSNIITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNE
                     VRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSV
                     RGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFA
                     EAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAA
                     FHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDI
                     INKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFY
                     LLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYY
                     KNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCN
                     LPASDHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSI
                     MGFLELLSGSGLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDI
                     PTLVQNTCHSFGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTE
                     GAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGL
                     MICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSIL
                     IADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFEL
                     TRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAH
                     IAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCI
                     HRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQ
                     KNN"
     gene            complement(3332..>4197)
                     /gene="yfdE"
                     /locus_tag="ACHUN9_003961"
     CDS             complement(3332..>4197)
                     /gene="yfdE"
                     /locus_tag="ACHUN9_003961"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0697221.1"
                     /translation="DVLAENFRPGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLK
                     DAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQR
                     GAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCG
                     NDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHE
                     VGVPVAPLLSVAEAINLPQTQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQ
                     HGEQIRQEFSS"
BASE COUNT         1264 a          948 c          838 g         1147 t
ORIGIN      
        1 caaaaagcaa tggacgcgct ggaagaaggt gaagtcgata tagtgttatc acatttagta
       61 gcttcgccgc ctcttaatga tgacattgct gcaaccaaac cactgataat tacctttccg
      121 gcgctggtaa ccacccttca cgattcaatg cgaccgctta cctcatcaaa accagtaaat
      181 attgctcgag tagcaaatta tccccctgac gaggtaattc atcaatcatt tccaaaagca
      241 acaattatct cttttacaaa tttatatcag gcattagcat ccgtctcagc cggacagaat
      301 gattacttta ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac
      361 tccttaaatg tagtgaaata ttataactca ccgcgtcaat ataatttttt cttgaccaga
      421 aaagaatctg tcgttcttaa tgaagtactc aatcgatttg ttgatgcttt gacaaatgaa
      481 gttcgctatg aagtatcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa
      541 ccattagaac tcactgaaca tgaaaaacaa tggattaagc agcatcccga tttaaaggtg
      601 ctggaaaatc cttactcccc tccctattct atgacggatg aaaatggctc ggttcggggc
      661 gttatggggg atattcttaa tattattact ttgcaaacag gtttaaattt ttctccgatc
      721 accgtttcac acaatatcca tgctggaaca cagctgaatc ccggcggatg ggatatatta
      781 cccggtgcta tttatagtga agatagagaa aataatgttt tatttgctga agccttcata
      841 acaacgcctt acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa
      901 ggaatgaaag ttgccattcc atattattat gagcttcatt cgcaattaaa agagatgtat
      961 ccggaggtag agtggataaa agtcgataac gccagcgctg catttcacaa ggtcaaggaa
     1021 ggcgaacttg atgctctggt cgcgacacag ttaaattcac gttacatgat cgaccattac
     1081 tatcctaatg aactttatca ttttcttatt cccggcgttc cgaatgcatc gctttcgttc
     1141 gcttttcctc gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatc
     1201 cccccaagcg aagttctgcg cctgaccgaa aaatggatta aaatgcccaa tgtgaccatt
     1261 gacacatggg acctctatag cgagcaattt tatattgtta cgacattatc cgttttatta
     1321 gttggcagta gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc
     1381 attcagggtg atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg
     1441 aatccaactt atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa
     1501 cattatttca ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactcc
     1561 ccctttaaag atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga
     1621 acaatataca cacaggtttt tgaaattgat aatggcatcg agaaaagatg cattaatcac
     1681 tggcatacat tatgcaatct gccagccagc gatcatgctg tttatatttg tggttggcaa
     1741 gatattacag agacgcgtga tttaattcat gcactcgaag tagagagaaa taaagcgatc
     1801 aatgcaactg tcgcaaaaag tcagtttctg gcaacaatga gtcacgaaat aagaacacca
     1861 ataagctcca ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa
     1921 cgggtggagg cgatttcact tgcctactcc accggacaat cactcctcgg cttaattggt
     1981 gaaatccttg atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc
     2041 gatatcccta ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa
     2101 tcgatcgcat taatttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct
     2161 caggcgttta agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag
     2221 ggggcagtaa aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa
     2281 atgacgatta tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa
     2341 cgctacagcc aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc
     2401 tgcaaagaat taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata
     2461 ggaacaacat ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag
     2521 gcaaaagcag aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat
     2581 catccgacca acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat
     2641 gaagccactg atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt
     2701 attactgacg ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag
     2761 caaaattctt ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa
     2821 aaagggttaa gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg
     2881 aaaacacatt taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt
     2941 gatatcgaag ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc
     3001 atgactttcc agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct
     3061 ggcgataaca gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg
     3121 aatttgcaaa agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac
     3181 agtaagcctg aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac
     3241 caggagattg ctgttttctg tcagaaaaat aactaaatag cggctcccac aatgttctaa
     3301 tgtgggagct atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt
     3361 ccccatgctg gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga
     3421 tttttatcgg attacccggc atcattattc ctccggcttc aatcaacata tttctcgcct
     3481 gagtttgtgg caaattaatg gcctcagcca cacttaataa cggcgcgacg ggcacaccaa
     3541 cttcatgtat tttggctaac caaacttcag ctgcctgcgt ttttaacgtt cgctcaatat
     3601 attgtttaag aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat
     3661 taaccagttc cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac
     3721 aacaaatcgt aatcggctta tcctgagtat cgaaaacatc aaaaggtgcc atgtagggat
     3781 ggcgatttcc caggcgttgt ggtgacttcc ctgtcgcaat atatgccatc agaccatgct
     3841 ccagaaaact cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct
     3901 tttcgcggcc ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat
     3961 ccgcaagaga ggtaccaaca cgcactggcg gagcatcagg gtaccctgtt tccatcatta
     4021 tcccactcat tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat
     4081 gtccgaaacc tgacgatgaa gcatatatga ggcgcgggtt gatttcttgt aacctttccc
     4141 atgaaaaccc cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcag
//
LOCUS       DAZOYS010000266         4096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOYS010000266 DAZOYS010000000
VERSION     DAZOYS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.268"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(258..905)
                     /gene="ychE"
                     /locus_tag="ACHUN9_003962"
     CDS             complement(258..905)
                     /gene="ychE"
                     /locus_tag="ACHUN9_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0697222.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1383..4058
                     /gene="adhE"
                     /locus_tag="ACHUN9_003963"
                     /gene_synonym="adhC"
     CDS             1383..4058
                     /gene="adhE"
                     /locus_tag="ACHUN9_003963"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0697223.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1136 a         1007 c          948 g         1005 t
ORIGIN      
        1 ttatttagca ctttcctcta ccaaaggcag tggaattact aatatcattt caattattct
       61 tcagcaagcc caggattatg atgtggcgaa aataacatga tggtgaggag aagcagttaa
      121 ggaaatcctt ataactaact gtaatttgag aagaatattt ttacctattc cttttgcttt
      181 tgataactgc gcgggaacaa agtaaaaata atgacgaagt aaacggagca tggcagctcc
      241 gtttcatttg aaaggaatta attaagcagg ccggggaaaa tccccttaat accagtaacg
      301 ataaattcaa tccccaatgc catcagcaat agccccataa tacgcgtaat cacgttgatg
      361 ccggtctggc gtaaaacccg taccagccac ggtgccatgc ggaacaatcc ccagcaacat
      421 aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat agctaatgct gtgataacgc
      481 gtaccccaga cgatggtaga actgatcgcc cctggccccg ccatcaacgg caacgccaat
      541 ggcaccacac caatgctttc acgtaccgcg gtttctgatt tttcttgctt gttctgttta
      601 tcctcgccaa gcttgccgct gatcatcgac atcgctatcg tcaccaccag gatgccccct
      661 gcgatacgga acgaatcaat tgatatacca aaaagttgca gaatcgtgtc gccgagaaaa
      721 agcgagatcc acaagataat ggccacagac aggttggctg taaggttagt tttgtttcgc
      781 gctgccgctg tctgataact ggtcatgctg ataaagacgg gaataatccc taccgggttg
      841 accagcgcaa ataacccgat gaaaaatttg aagtaaacgg gaaaatccaa aaaggtctga
      901 atcacggtta gctccgaagc aaaagccgga taatgttagc cataaattaa ggttgaaaag
      961 acgcgctgac aatacgcctt ttgacagcat ttttcacctc ctaactactt aaaattgcta
     1021 tcattcgtta ttgttatcta gttgtgcaaa acatgctaat gtagccacca aatcatacta
     1081 caatttatta actgttagct ataatggcga aaagcgatgc tgaaaggtgt cagcttagca
     1141 aaaatttgat ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc
     1201 tagaagtgac gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt
     1261 gagtgtgagc gcgagtaagc ttttgatttt cataggttaa gcaaatcatc accgcactga
     1321 ctatactctc gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca
     1381 ttatggctgt tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc
     1441 agcgtgaata tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc
     1501 tggctgctgc agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg
     1561 gtatcgtcga agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct
     1621 ataaagatga aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta
     1681 tcgctgaacc aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg
     1741 ctatcttcaa atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc
     1801 cgcgtgcaaa agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg
     1861 ccggtgctcc gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg
     1921 cactgatgca ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta
     1981 aagccgcata cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg
     2041 ttatcgatga aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct
     2101 tcgacaacgg cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg
     2161 acgctgtacg tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga
     2221 aagctgttca ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc
     2281 cagcctataa aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga
     2341 tcggtgaagt gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga
     2401 ctctggcaat gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg
     2461 ttgctatggg cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg
     2521 ctcgcgtttc ttacttcggt cagaaaatga aaacggctcg tatcctgatt aacaccccag
     2581 cgtctcaggg tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg
     2641 gttgtggttc ctgggggggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca
     2701 acaagaaaac cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta
     2761 tctacttccg ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca
     2821 aacgtgcgct catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca
     2881 cttccgtact gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc
     2941 cgaccctgag catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga
     3001 ttatcgcgct gggtggtggt tccccgatgg acgccgcgaa gattatgtgg gttatgtacg
     3061 aacatccgga aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta
     3121 tctacaagtt cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg
     3181 gtacaggttc tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat
     3241 atccgctggc agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta
     3301 tggacatgcc gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg
     3361 aagcttatgt ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga
     3421 aactgctgaa agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc
     3481 gtgaacgtgt tcacagtgca gcaactatcg cgggtatcgc atttgcgaac gccttcctgg
     3541 gtgtatgtca ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg
     3601 caaacgccct gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc
     3661 agactgcatt cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg
     3721 accacttggg tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg
     3781 catggctgga aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg
     3841 ttcaggaagc agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc
     3901 agtgcaccgg cgctaacccg cgttacccgc tgatctccga gctgaaacag atcctgctgg
     3961 atacctacta cggtcgtgat tatgtagaag gtgaaactgc agcgaaaaaa gaagccgctc
     4021 cggctaaagc tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaata
     4081 taaacggccc cttctg
//
LOCUS       DAZOYS010000267         4089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOYS010000267 DAZOYS010000000
VERSION     DAZOYS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.269"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="ACHUN9_003964"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="ACHUN9_003964"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0697224.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="ACHUN9_003965"
     CDS             932..1384
                     /locus_tag="ACHUN9_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0697225.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="ACHUN9_003966"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="ACHUN9_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /gene="yicS"
                     /locus_tag="ACHUN9_003967"
     CDS             complement(2816..3109)
                     /gene="yicS"
                     /locus_tag="ACHUN9_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0697226.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4089
                     /gene="nlpA"
                     /locus_tag="ACHUN9_003968"
     CDS             3331..>4089
                     /gene="nlpA"
                     /locus_tag="ACHUN9_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR0697227.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          900 a          906 c         1077 g         1206 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt cctgcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcggt gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaatca
//
LOCUS       DAZOYS010000268         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOYS010000268 DAZOYS010000000
VERSION     DAZOYS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.270"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            97..705
                     /locus_tag="ACHUN9_003969"
     CDS             97..705
                     /locus_tag="ACHUN9_003969"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0697228.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            803..2194
                     /gene="selA"
                     /locus_tag="ACHUN9_003970"
     CDS             803..2194
                     /gene="selA"
                     /locus_tag="ACHUN9_003970"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0697229.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2191..4035
                     /gene="selB"
                     /locus_tag="ACHUN9_003971"
     CDS             2191..4035
                     /gene="selB"
                     /locus_tag="ACHUN9_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0697230.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          904 a         1031 c         1208 g          918 t
ORIGIN      
        1 tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg
       61 ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc
      121 agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt
      181 attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa
      241 gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat catcgctgaa
      301 tatattgagt taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg
      361 cgggtgcgca aaattgaggc gctggcggat ggcattatgg atgccgggct ggtatcggtg
      421 cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg ccagcgggag
      481 aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc
      541 gatacggtca atctggcgac tatcgccatt gcctgtgctg tcggatatct caatttccgc
      601 cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg
      661 tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct
      721 cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg
      781 gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc
      841 ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca
      901 cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga
      961 cagccagacg cttcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac
     1021 gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac
     1081 caaccttgga cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg
     1141 ttcgccagtg accctcgagt acgatctgga cgacgccgga cgcggacatc gcgatcgggc
     1201 gctggcgcaa ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg tcaataacaa
     1261 tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg tggtagtatc
     1321 tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc
     1381 aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca
     1441 ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca
     1501 ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt
     1561 tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc
     1621 gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga gcttctccgg
     1681 cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag agataatcgc
     1741 ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc
     1801 gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac
     1861 cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc
     1921 tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca
     1981 gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc
     2041 ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt
     2101 gccgatgatt ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga
     2161 tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac
     2221 cacggcaaaa caaccttatt gcaggtgatt actggcgtaa atgctgaccg tctgccggaa
     2281 gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt
     2341 cgcgtgccta gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg
     2401 ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg
     2461 cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg
     2521 ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag
     2581 gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa
     2641 ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc
     2701 agccaacata gtgtccgcct cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg
     2761 gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact
     2821 ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc
     2881 gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt
     2941 aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc
     3001 gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca ccacgccgcc
     3061 agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct ggtgttcgac
     3121 acgccgctgt ggctggcgga taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac
     3181 acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg
     3241 gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga tgcgttatct
     3301 gttcatctgg aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac
     3361 ggcgaaggga tgcgcgaatt gctgcaacag cctggctata ttcaggctgg ttatagcttg
     3421 ttgaatgcgc cggttgccgc ccgctggcag cggaaaattc tcgacacatt agcgacttat
     3481 catgagcaac atcgcgatga acctggtcct gggcgcgaac gtctgcgacg tatggcgttg
     3541 ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga aagcggcgac
     3601 atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag
     3661 cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt
     3721 gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct acgccaggcg
     3781 gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt
     3841 gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg
     3901 gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt
     3961 gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta
     4021 tttccggaaa aataaggaaa tgattaatta atttttaaaa t
//
LOCUS       DAZOYS010000269         4045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOYS010000269 DAZOYS010000000
VERSION     DAZOYS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.271"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUN9_003972"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUN9_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0697231.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUN9_003973"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUN9_003973"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0697232.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUN9_003974"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUN9_003974"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0697233.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUN9_003975"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUN9_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0697234.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1141 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAZOYS010000270         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOYS010000270 DAZOYS010000000
VERSION     DAZOYS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.272"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..432
                     /locus_tag="ACHUN9_003976"
     CDS             <1..432
                     /locus_tag="ACHUN9_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697235.1"
                     /translation="CKEEADEINKKSMSRVKKHKERLMEITPEIFFRFLSEKGVSSVC
                     PSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQSELIKENIKHYVSYAFLGDVKSI
                     PDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKAQDGDNG"
     gene            533..970
                     /locus_tag="ACHUN9_003977"
     CDS             533..970
                     /locus_tag="ACHUN9_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR0697236.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            1163..2143
                     /gene="btuC"
                     /locus_tag="ACHUN9_003978"
     CDS             1163..2143
                     /gene="btuC"
                     /locus_tag="ACHUN9_003978"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0697237.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            2206..2757
                     /gene="btuE"
                     /locus_tag="ACHUN9_003979"
     CDS             2206..2757
                     /gene="btuE"
                     /locus_tag="ACHUN9_003979"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0697238.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            2757..3506
                     /gene="btuD"
                     /locus_tag="ACHUN9_003980"
     CDS             2757..3506
                     /gene="btuD"
                     /locus_tag="ACHUN9_003980"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0697239.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            3584..>4043
                     /locus_tag="ACHUN9_003981"
     CDS             3584..>4043
                     /locus_tag="ACHUN9_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0697240.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT          979 a          885 c         1119 g         1060 t
ORIGIN      
        1 tgcaaagaag aagctgatga aatcaataag aagtcgatga gtcgtgtcaa aaaacataaa
       61 gagcggctga tggagattac gccagaaatc ttcttccgtt tcttgtctga gaagggggtc
      121 tccagtgtct gcccttcatg tggatcgtcg cgattatctg ttccagaaag tatggatctt
      181 tgctgggata aaaataagaa accagaaaat tttaacaatc tacccctgga ggaacagagt
      241 gagttaatta aagagaacat aaagcattat gtatcttatg cttttttggg tgatgttaag
      301 agcatacctg atatgcgcaa aacttattac acgctgcatt gcctgaattg tggttacctg
      361 agcctttatc gtacgtctgc ggtgctgaag tggttggaga aagacaaagc gcaggatggt
      421 gataatgggt aatgtagcaa gaaatctttt cggtaattca gcaggtagtg tgtcgcattc
      481 tgagcgtgat gtgctttatc atggtggcga tggcggcagt agtggaggcg gaatgtcaga
      541 taaacttgaa aggcgaattg agcggctcga aggtgattta tcgctaacaa gaaacgacct
      601 tgcgacgctt gctgaacgca ctacaaacct ctcaactaaa gccgatgttg gtgaggtgaa
      661 aggtgaactc aaagcagaca tagcacatct gaaaggtgat cttgaatgcg atattgcgaa
      721 tctgaaaggt gagcttaaat ctgatacagc taacctgaaa gagcagctca aatcagacat
      781 taacagcctg aagggtgagc ttaccgaagc gatggataaa cgctttgaca agattatgga
      841 tgagatgaat cggcggtttg acaaggttga tgataatacg aagtggcgtt ggagtggcat
      901 tattgtgcca gtgtgcacaa ccattttcac agcggcggtt gctgtatttg ttgctaaatt
      961 tgttggctga tgatccacaa attgaccaca tctctgttat ttgttgtggt tggctgtgtt
     1021 tttgtgtgtc tgtaagtctt tgataattac ctaacttatt gatttttgtt tgtgtttatg
     1081 gccgctctgc ggcctttttt cttttcactg tcgaagagtc accgtaaaat caacgccatg
     1141 acacttcagc agaacggata ccatgctgac acttgcccgc caacaacagc gacaaaatat
     1201 tcgctggtta ttatgcctgt cagttttgat gctgctggcg cttctcttaa gcctttgcgc
     1261 cggtgaacaa tggatttcgc caggtgactg gtttactcct cgtggcgaac tgttcgtctg
     1321 gcaaattcgc ctgccacgta cgctggctgt attgctggtt ggtgcggcgc tggctatatc
     1381 cggcgctgta atgcaggcgt tgtttgaaaa tcctctggca gaacctggac tacttggcgt
     1441 ctctaacggc gcaggcgtgg ggcttatcgc cgcggtattg cttgggcaag ggcaactccc
     1501 caactgggcg ctagggctgt gtgcgattgc tggcgcgctt atcatcactt taatactctt
     1561 acgtttcgcc cgtcgtcatc tttcgaccag tcggttattg ctggctggcg ttgcattagg
     1621 gattatctgt agcgcactaa tgacgtgggc tatctacttt tccacctctg ttgatttacg
     1681 tcagctgatg tactggatga tgggcggttt tggcggcgta gactggcggc aaagctggct
     1741 gatgctggca ttgatccccg tgttgttgtg gatctgttgt cagtccaggc cgatgaatat
     1801 gttagcactt ggcgagatct cggcgcggca actgggttta cccctgtggt tctggcgcaa
     1861 tgtgctggtg gcagcgaccg gctggatggt tggcgtcagt gtggcgctgg cgggtgctat
     1921 cggctttatt ggtctggtga tcccacatat tcttcggttg tgtggtttaa ccgatcatcg
     1981 cgtattactt cccggctgcg cgctggcagg ggcgagcgca gtgctgctgg ccgatattgt
     2041 agcgcgcctg gcattagctg ccgcagagct gcctattggc gtggtcaccg caacgttggg
     2101 tgcgccggtg tttatctggt tattgttaaa agcaggacgt tagccgcaaa aagacggtct
     2161 atgattaaaa gctaagattt ttactgacca cacccaggag aaacgatgca agattccatt
     2221 ctgacgaccg tagtgaaaga tatcgacggt gaagtgacca cgctggagaa gtacgccggt
     2281 aatgtgctgt tgattgtcaa tgtcgcctca aagtgtggct taacgccgca atatgagcag
     2341 ttggagaata ttcagaaagc ctgggccgat cgaggttttg tggtgctggg attcccgtgc
     2401 aaccagtttc tggaacaaga accgggcagc gatgaagaga ttaaaactta ctgtaccacc
     2461 acatgggggg tgacgttccc gatgttcagt aagattgaag ttaacggcga aggacgccat
     2521 ccgctgtatc aaaaattgat tgccgcagcg ccgaccgcag tcgcgccgga agagagcgga
     2581 ttctatgccc gtatggtcag caaaggtcgt gcaccgctgt accctgatga tattttatgg
     2641 aattttgaga aatttttagt tggcagggac ggattggtca tccagcgttt ttccccggat
     2701 atgacgccgg aagaccccat tgtgatggaa agcattaaac tggcgttggc aaaataatgt
     2761 ctattgtgat gcagttacag gatgttgcgg aatctacccg cctggggcca ctttctggcg
     2821 aggttcgggc tggggagatc ctgcacctgg tggggccgaa tggcgcgggt aagagtacct
     2881 tactggcacg aatggccgga atgaccagcg gtaagggaag tattcagttc gcggggcaac
     2941 cactggaagc atggtccgca acaaaactcg cgctgcatcg cgcctatctt tcacaacagc
     3001 agacgccgcc gtttgcaatg ccggtctggc actatctgac actgcatcag cacgataaaa
     3061 cgcgtaccga actactgaat gatgtcgcag gggcgctggc tcttgatgac aaactcggac
     3121 gtagcaccaa tcaactttcc ggcggtgaat ggcaacgcgt acgtcttgct gcggtggtgc
     3181 tgcaaatcac accacaagcc aatcccgcag gccaattgct gcttcttgat gagccgatga
     3241 acagtcttga tgttgcgcaa caaagtgcgt tagacaaaat tctgagcgct ctgtgtcagc
     3301 aaggactggc gattgtgatg agcagtcacg atctcaacca cacattgcgt catgcgcatc
     3361 gggcgtggtt gctaaaaggt ggaaaaatgc tggccagtgg acgcagagaa gaggtgctca
     3421 cgccgccaaa tctggcgcag gcctatggga tgaattttcg ccgtctggat atcgaaggtc
     3481 acagaatgct gatttcgacc atctgatttc attagtatct tgaaaatcgg gtaattacga
     3541 cgctaaatta atgcaagaat aaaaaacaga ggaaccgtac ggaatgcgtt tctgccttat
     3601 tttgatcaca gcactgtttc tggccgggtg tagccaccat aaagcaccgc cgccaaatgc
     3661 cagactttct gattcgatta ccgttattgc cggtttgaac gaccagctac aaagctggca
     3721 tggcacgccg tatcgttatg gtggcatgac gcggcgcggt gtggactgtt cgggatttgt
     3781 ggttgtaacg atgcgcgatc gtttcgattt gcagctgccc cgagaaacca aagaacaagc
     3841 ctctatcggc acgcaaattg ataaagacga gttgctgcct ggtgacctgg tctttttcaa
     3901 aacgggttcc ggacaaaatg gtttgcatgt aggtatttac gataccaaca accaatttat
     3961 ccacgcctct accagcaagg gagtgatgcg ttcctcactt gataatgtct attggcagaa
     4021 aaatttctgg caggcgagac gaa
//
LOCUS       DAZOYS010000271         4041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOYS010000271 DAZOYS010000000
VERSION     DAZOYS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.273"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHUN9_003982"
     CDS             376..483
                     /locus_tag="ACHUN9_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0697241.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUN9_003983"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUN9_003983"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0697242.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUN9_003984"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUN9_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0697243.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUN9_003985"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUN9_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0697244.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1032 a         1011 c         1052 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga a
//
LOCUS       DAZOYS010000272         3948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOYS010000272 DAZOYS010000000
VERSION     DAZOYS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.274"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            32..970
                     /locus_tag="ACHUN9_003986"
     CDS             32..970
                     /locus_tag="ACHUN9_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR0697245.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1026..2315
                     /locus_tag="ACHUN9_003987"
                     /pseudo
     CDS             1026..2315
                     /locus_tag="ACHUN9_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2312..2605
                     /locus_tag="ACHUN9_003988"
     CDS             2312..2605
                     /locus_tag="ACHUN9_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0697246.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2770..3948
                     /gene="fadK"
                     /locus_tag="ACHUN9_003989"
                     /pseudo
     CDS             2770..3948
                     /gene="fadK"
                     /locus_tag="ACHUN9_003989"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1004 c         1089 g          926 t
ORIGIN      
        1 gccctgaact gaagcctacg gagaagaaac gatgagtcaa ttaaacagcg tctgggtctt
       61 tagcgataat cctgaacgtt atgctgaact gtttggcggc gcgcagcaat ggggccaaca
      121 ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt atggtccaaa
      181 atgtatttat gttcttgagc aaaatgatgc gctgcaacgt actgaaaatt acgccgaaag
      241 cattgctgcc ctgctgaaag ataacccccc cgctatgttg ctgctggccg cgacgaaacg
      301 tggtaaagcg ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg tgaacgatgc
      361 cacggcagtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt atggcgggtt
      421 agcgttcgct caggaaaaga tcaacagccc gctggcgatc attacccttg cacccggtgt
      481 tcaggaaccg tgcaccagtg atacctctca tcagtgcccg acagaaacgg taccttatgt
      541 tgccccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagcggcaa gcagcgtgga
      601 cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcagcgc aggatgacct
      661 gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt cacgtccaat
      721 tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg gcgtgttgct
      781 gaaatccgat ctctacctga cgctggggat ctccgggcag atccagcata tggttggcgg
      841 caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa tcttcaatta
      901 tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga ttagccagtt
      961 gagccgctaa ttcccctttc aaccacatct cccgctgtaa acgcagcggg aagggagcat
     1021 aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg tgcgggcgtt gctggtagcg
     1081 ttgccgcact ggtcatggcg cgagccgggc tggatgtcct ggtgatagaa cgcggcgaca
     1141 gtgccggatg taaaaacatg accggcgggc gtctttatgc ccacacactt gaagcaatca
     1201 ttccaggctt tgcagtatca gcgccggtag aacgcaaggt cacacgcgag aaaatctcct
     1261 tcttaaccga agaaagcgcc gttaccctcg attttcaccg cgagcagcca gatgttccgc
     1321 aacacgcatc ttataccgta ttgcgtaatc gtctggaccc gtggttgatg gaacaagccg
     1381 agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga tgcgctggtt cgtgaaggaa
     1441 acaaggtaac tggcgtgcag gctggggatg atattctcga agcgaatgtg gtgattctgg
     1501 ctgatggcgt taactcgatg cttggccgct cgctgggaat ggttcccgct tccgatccgc
     1561 atcattacgc tgttggtgtt aaagaggtta ttggcctcac accagaacag atcaacgatc
     1621 gctttaatat tacgggcgag gaaggtgccg cctagctgtt tgccggttcc ccttctgacg
     1681 gcctgatggg tggcggattc ctctatacca acaacgattc cgtatcgttg gggctggttt
     1741 gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc gcaaatgctg gaagatttta
     1801 aacaacaccc cgccattcgc ccactaatta gcggcggcaa actgcttgaa tattccgcgc
     1861 atatggtgcc ggaaggcggt ctggcgatgg tgccgcaact ggttaacgag ggcgtgatga
     1921 tcgttggtga cgccgcaggc ttctgtctga atttgggttt tacggtccgc ggcatggatt
     1981 tagccattgc atcggctcag gctgccgcca caacggtgat cgccgccaaa gaacgcgagg
     2041 atttctccgc cagcagtctg gcgcaataca agcgtgagct ggaacaaagc tgcgtcatgc
     2101 gtgatatgca gcattttcgc aagatcccgg cgctgatgga aaacccgcgc ctgtttagcc
     2161 aatacccacg aatggtagcc gacatcatga acgagatgtt caccatcgac ggcaaaccaa
     2221 accagccggt acgaaaaatg atcatgggac atgcgaagaa aattgggctg atcaacttgc
     2281 tgaaagatgg cattaaggga gcaaccgcgc tatgagccag aacgctacgg ttaacgtcga
     2341 catcaaatta ggcgtcaata aattccatgt tgatgagggc cacccgcata tcattctggc
     2401 ggcaaatccc gacatcaatg aattccgtaa attaatgaaa gcctgccctg ccggacttta
     2461 taagcaggat gacgccggca acattcattt tgattctgcc ggttgtctgg agtgcggcac
     2521 ctgtcgggtg ctgtgcggta acactattct cgaacagtgg caatatcccg caggcacctt
     2581 cggtattgat tttcgctacg gctaatcatg catcccacag gcccgcatct cgggcctgat
     2641 gttctgtttc gagagtccaa catgaaagtg acattaacgt tcaacgaaca acgtcgtgcg
     2701 gcgtatcgtc agcaagggct atggggcgat gcttcgctgg ccgattactg gcagcagacc
     2761 gctcgtgcga tgccagacaa aattgccgtg gtcgataatc atggtgcatc gtacacctat
     2821 agcgcgctcg atcacgccgc gagctgtctg gcaaactgga tgttagcgaa gggtattgaa
     2881 tcaggcgatc gcatcgcatt tcaactgcca ggctggtgtg aatttaccgt tatctatctt
     2941 gcctgcctga aaaccggtgc ggtttccgtg ccgctgttgc cttcctggcg ggaagcagaa
     3001 ctggtgtggg tgctcaataa gtgtcaggca aaaatattct ttgcaccgac gttgtttaaa
     3061 caaacgcgtc cggtagattt aatcctgccg ctgcaaaatc agcttccaca actacaacaa
     3121 attgtcggcg tggacaaact ggctcccgcc acctcttccc tctcattaag tcagattatc
     3181 gccgacaata tcccactgac cacggcgata acgacccacg gcgatgaatt agctgcggtg
     3241 ctgtttacct ccggaaccga gggtctgcca aagggcgtaa tgctaacgca taacaatatt
     3301 ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga cctggcagga tgtctttatg
     3361 atgcctgcgc cacttggtca cgcaacggga tttctgcatg gcgtaacagc accattttta
     3421 attggtgctc gcagcgtgtt gttagatatt ttcactcctg atgcatgtct cgcgctgctt
     3481 gagcagcagc gttgcacctg tatgctcggc gcaacgccgt ttgtctatga tcttttgaat
     3541 ttactagaga aacaacccgc agacctttca gcgctgcgct tctttctttg cggcggtacc
     3601 acaatcccca aaaaagtggc gcgcgaatgc cagcagcgct ggcattaaat tattaagtgt
     3661 ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat ctcgatgatc ctttgtcgcg
     3721 ctttatgcac accgatggtt acgctgccgc aggtgtagag attaaagtgg tcgatgacgc
     3781 acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc tcgcgtggcc ccaatgtgtt
     3841 tatggggtat tttgatgaac ctgaattaac cgcccgtgcg ctggatgaag aaggctggta
     3901 ttacagcggc gatctctgcc gtatggatga ggctggctat ataaaaat
//
LOCUS       DAZOYS010000273         3938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOYS010000273 DAZOYS010000000
VERSION     DAZOYS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.275"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="ACHUN9_003990"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="ACHUN9_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0697247.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHUN9_003991"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHUN9_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0697248.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHUN9_003992"
     CDS             complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHUN9_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0697249.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHUN9_003993"
                     /pseudo
     CDS             complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHUN9_003993"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1094 a          972 c          862 g         1010 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     1861 aataaagtta gtgacataac tacggtatgt ataattcacc agcgtgccgg gtggacaaat
     1921 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     1981 attggtgcta ttaaagacat ttgagagatc atagtgttca tcttctgcta cgccagtact
     2041 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca ttgccaataa
     2101 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     2161 cgaaataaca gtgacggctt gctgtaagct ttgctttggc aatacatagt gcgcaatgca
     2221 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     2281 taaataaact tgcccgtcat cacccaccat actggttatt ccggtactgt tttcccgtac
     2341 cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg cccgcacgcc
     2401 tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa cgctgctaat
     2461 atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga tacggttata
     2521 ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta aaatgccagt
     2581 ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat tggtatcccc
     2641 taaaggctgg ctcagtgtta tgccattttc atgcccgacc acaccgccag acagctgcca
     2701 gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtac cgtaagtggc
     2761 ctgccagttt gccgtggcgc tgcccgtgta cccattattc gaggtatcgc cctggcttac
     2821 gtgataactc aggttgtggc cttccagtaa ggtcccacca acgcctgcca gccagctatt
     2881 ttgcccgttg ctgttacggt tagcgttcaa ggaggcataa gcgcggtcga gcgcattttc
     2941 ccgggtgtag cgacgtttgg tcaaaacatt gaaaggcact gaaacattaa gtccgacaat
     3001 atgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg aataactgat
     3061 gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg aagtattcca
     3121 gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg attgtgaaat
     3181 atttaactgc aaacggtctt tgcggctaaa gcgtaaattg tggtaattca cgattatcgg
     3241 ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca ttcgacgata
     3301 cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca ttaactgaaa
     3361 attggtgccg aaggtgtaca tcgatttcgc atagaggaag cgaatggaat ccccctcatg
     3421 acgactgtcg tcggctaact ggctgcgcgc atgggtcaca tccagcgaca ctgcgcccca
     3481 gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag ataattgcgt
     3541 cccgccgtag gcagtaaatt cctgtggtaa accaccgagt gccgtgccct gaaaaaagaa
     3601 aggtgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt caaatttgaa
     3661 acgcccttcg cgttgtaaaa tcggcactgt tgaatacgga attgtgtaat tctgccatcg
     3721 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atcagtaatt
     3781 tcaaaagcac cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3841 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatct
     3901 ggatacatat tatcggaaga ataaagccgt acaccacg
//
LOCUS       DAZOYS010000274         3936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOYS010000274 DAZOYS010000000
VERSION     DAZOYS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.276"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1370
                     /locus_tag="ACHUN9_003994"
     CDS             <1..1370
                     /locus_tag="ACHUN9_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR0697250.1"
                     /translation="SNLSDLSSLSTARTNLQVNRLTQGATYTDLMSNDLTNRIRIQDN
                     GAWGASTGTGWIALGIGQGGTGATDALSARLNFQVDRFNQGSNLTTIKSADSSAALVV
                     YDGGQWGSATYIAGAFTGWKALAIPQGGTGVKTQAELWPAIRPTGATSLAGDPVSDSD
                     ATTKRWVENLLSSRTSVSPQWDVIASSPSSTAIGSYVTGGRIRSICNIGTSTEISGGF
                     RVGKTMGQTGQADLTLFISENGAEDKLWGLKGPTGTILTDVQSGWMTNTAVSGPTDAN
                     TITFNGMYAGPGDSGINYSEKYSPLLHMQRYPGANRSQLQIDGGGTLRVRGGNGTTWF
                     GWLVMQAQGTSGREFKRDITPANPVEALDRIMSQDLVTFIYKDDEQERVRFGVIAEDA
                     ELTAPQYIKHDEETVEIEGEIVTRDHPRVDLNPIVMDLMGAIQAQQEEINQLKDLVQQ
                     LLNNK"
     gene            1429..1902
                     /locus_tag="ACHUN9_003995"
     CDS             1429..1902
                     /locus_tag="ACHUN9_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0697251.1"
                     /translation="MAMDIFSGANLTVEVGSSAGKTVATDFVEVPEVNTFSTSGFEST
                     VISVKTFNNAYDRKLLGTKSIPDISLAVNYLPDNAVHQNLEQLADDQKRCQVKLSYFT
                     DATKKEGFFVIYTCFVSSTTIGGDKDEVVTKTFTLAVDGEAIDSGLITPKPESGE"
     gene            1954..2253
                     /locus_tag="ACHUN9_003996"
     CDS             1954..2253
                     /locus_tag="ACHUN9_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697252.1"
                     /translation="MNLNELKKKLQPELKPHEVMGEIIFIHRPNGQDFQKCTDVAKTL
                     ILCVKDENGDPIFAEEDIDGRINVNSIDFVIQNEIYSAIIALINTAKPVDEVEKK"
     gene            2286..2648
                     /locus_tag="ACHUN9_003997"
     CDS             2286..2648
                     /locus_tag="ACHUN9_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697253.1"
                     /translation="MVNKRGLSPEEYFSLDPDVLNMLMIYDTFIEPSGTQIEMMKHAY
                     QCYYTTISNGNLTPEARKSIKVQDFDFLDVLSDSTKSTSEKAEERKLKTKEAQSNDIK
                     SIGELIKSQVLGKKKNGK"
     gene            2638..>3936
                     /locus_tag="ACHUN9_003998"
     CDS             2638..>3936
                     /locus_tag="ACHUN9_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697254.1"
                     /translation="MANNNRIRVDITGDSSGLQRALRSGTESLEEFGSTAGGIVEEFT
                     GRITGMAGGFGTAMTGIAGAAAIGIGGLAALVESSREYVREMNQISKSTGLSVVQLQQ
                     LSAAFSGLGLEIDKFGDFNKDTLDKLGDAFRAGGGVSDDLKEYGLNLQDYNKYLKQAD
                     GGMQAVIHTFYAMRDAGKSQSEIVNVMETLASDSSHMISTLQQFNNEAQATAYIQSQN
                     AEVTNDAAEKYAEFDKNLGKLTTSIKGTIADGLSPLVNAMNSVYDAANQKPHEAGLFE
                     DLNERIKTSKGSLQDMLDIWEKLRTAGALNYQGAALNTGIMDDKGNKGNEFAQAKARL
                     EELANNLKSDVAVATAPTGGWVDQAKEAEDAAKKLEQQRKQAEAAQKAADAKRLQAQR
                     NLEAALAQVGEDGFQVRLQQFDRQQKALLKTITDSAQVLGI"
BASE COUNT         1237 a          705 c          921 g         1073 t
ORIGIN      
        1 cgagtaacct ttctgatcta tccagtctct ctacagcacg tactaaccta caggtgaacc
       61 gtttgactca gggtgctacg tacaccgatc tgatgagtaa tgacctgact aaccgtatcc
      121 gaattcagga taacggggca tggggtgcaa gtactggtac tggatggatt gctttaggta
      181 taggtcaggg ggggacaggg gctacagatg ctttgagtgc tcgcctaaac tttcaggtgg
      241 atcgctttaa tcaaggtagt aatttaacaa ctattaaatc cgctgattct tccgctgcat
      301 tagtggttta tgatggaggg caatggggtt cagctacata tattgcgggt gctttcactg
      361 gctggaaagc attagcaatt ccgcagggcg gtactggtgt taagactcag gctgagttat
      421 ggcctgcgat tcgtcctacg ggtgcaactt ctttagcagg agatcctgtc tctgattctg
      481 acgcgactac caaacgctgg gttgaaaact tgctgagtag tcgtacttca gttagtcctc
      541 aatgggatgt tattgcttct tccccttcct ctacggcaat tggtagttat gtaactggag
      601 ggcgtattcg ttcgatatgc aacattggta catcaactga aatatctggt ggttttcggg
      661 ttggtaaaac gatgggacag actggacagg ctgatctcac cctttttatt tctgaaaatg
      721 gtgcagagga taaactatgg gggctaaaag ggccgactgg aacgatccta actgatgttc
      781 aatcaggatg gatgactaat accgctgttt cgggaccgac agatgccaat accatcacct
      841 ttaacggtat gtatgcaggg ccaggtgact caggtattaa ctatagtgaa aaatattccc
      901 ctttattgca catgcaaaga tacccagggg caaatcgctc tcagcttcag atagacggtg
      961 gtggtacttt aagagtacga ggcgggaacg gtacaacatg gtttggatgg ttagtaatgc
     1021 aagctcaagg tacatcaggt cgtgagttta aaagagacat aactccagca aatccagttg
     1081 aagcattaga tcggataatg tcacaagatt tagtaacgtt tatctacaag gatgatgaac
     1141 aagaaagggt taggtttggt gttatcgccg aagatgctga gttgactgct ccgcagtaca
     1201 ttaagcatga tgaagaaaca gttgaaatag aaggggagat tgtcactcgt gatcatccac
     1261 gtgttgattt aaatccaata gtgatggact tgatgggagc tattcaagcc caacaagaag
     1321 aaattaatca gctaaaagat ttagtacaac agctattaaa taataaataa atataaacgg
     1381 ggtgaacgga ttcactccaa taaaattttc ttataaggat ataaaactat ggctatggat
     1441 attttttccg gtgctaacct tactgtagaa gtaggttcaa gtgctggtaa aacagttgct
     1501 actgactttg tagaagtacc agaggtaaac accttcagta cttcaggctt tgaaagtact
     1561 gtaattagcg taaaaacatt caataatgct tatgatcgta agctattggg tactaagtca
     1621 atcccggaca tttctcttgc agttaactac ttaccagata atgcagtaca tcagaattta
     1681 gaacaacttg cagacgatca gaaacgttgt caggtaaaac taagctattt taccgatgca
     1741 actaaaaaag aaggtttctt tgtaatttat acctgctttg tttcaagtac cactatcggt
     1801 ggtgataaag atgaagtagt aactaagact ttcacccttg cagttgatgg tgaagcaatt
     1861 gattcaggtc tgattactcc aaaaccagaa tcgggcgaat aataataaat aaagggaagg
     1921 tgctcaactt tccctttttt attggagatg aaaatgaatt taaacgaatt gaagaaaaaa
     1981 ctacaaccag aattaaaacc acatgaagta atgggtgaaa tcatctttat tcatcgtcca
     2041 aatggtcagg atttccagaa atgcactgat gtggctaaaa ctctaatcct atgtgtaaaa
     2101 gatgaaaatg gtgatcctat cttcgcagaa gaagatattg atggacgaat taatgttaac
     2161 tctattgatt ttgtaatcca gaatgaaatt tactcagcaa ttattgcttt aatcaatact
     2221 gcgaaacctg tagatgaagt agaaaaaaag taagaggtga tatcgaatta aagtactttt
     2281 gtaagatggt taacaaaaga ggcttatcac ctgaagaata ctttagttta gatcctgatg
     2341 tactcaatat gcttatgatc tacgatactt ttatagaacc atccggtacg cagattgaaa
     2401 tgatgaaaca tgcgtaccag tgttactaca ccactatcag taatggcaac ctaacaccag
     2461 aagcaaggaa aagtatcaag gtacaggatt ttgattttct tgatgtgctt agtgatagta
     2521 ctaagagtac ttcagagaaa gctgaagaaa gaaaattaaa aactaaagag gctcaatcca
     2581 atgatattaa atctataggg gagttaatta aatctcaggt attaggaaag aaaaagaatg
     2641 gcaaataata atagaatacg tgtagatatt acgggtgatt cttccggttt acagcgtgct
     2701 ttaaggtctg gtactgaatc attagaggaa tttggtagta ctgctggtgg cattgttgaa
     2761 gagttcacag gccgaataac tggtatggct ggcgggttcg gtactgctat gaccgggatc
     2821 gcgggtgctg cggctattgg tattggtggc ctggcagcat tagtagaatc atcacgtgaa
     2881 tatgtgcgtg aaatgaacca gatcagtaag agtaccggat tgtccgtggt tcagttacaa
     2941 cagctatcag cggcgtttag tggcctcggt cttgagattg ataagtttgg tgatttcaac
     3001 aaggatacct tggataagct cggtgatgct tttcgtgctg gcggtggtgt atccgatgat
     3061 cttaaagaat atggactcaa cctacaggac tataacaagt acttaaaaca ggctgatggt
     3121 ggtatgcagg ctgtgatcca caccttttac gccatgcgtg atgcaggtaa atcacagagt
     3181 gaaatagtta acgtaatgga aacgttagcc agtgactcaa gccacatgat tagtactctt
     3241 cagcagttca ataatgaggc tcaagctaca gcctatatcc agtcacaaaa tgctgaagta
     3301 actaatgatg ctgctgaaaa atatgctgaa ttcgataaaa accttgggaa acttactacc
     3361 agcattaaag gtactattgc tgatggccta tcacctttag ttaatgccat gaacagtgtc
     3421 tatgatgctg caaatcaaaa acctcacgag gcaggtttgt ttgaagatct gaatgaacgt
     3481 attaaaactt ccaagggttc tctacaggat atgttggata tctgggaaaa attacgtaca
     3541 gcaggggcat taaactacca gggtgcagca ctcaatactg gcatcatgga tgataagggc
     3601 aacaagggta atgagttcgc tcaggctaag gcacgtttag aagaactggc aaacaaccta
     3661 aagtccgatg ttgcggttgc taccgctcct actggcggat gggttgatca ggcaaaagaa
     3721 gctgaagatg cagcgaagaa gttagaacaa caacgtaaac aggcagaagc ggcacaaaag
     3781 gcggcagatg caaaacgcct acaggctcaa cgtaatctgg aagctgctct tgctcaagtt
     3841 ggggaagatg gttttcaggt acgtctacag cagttcgaca gacaacaaaa ggcacttctt
     3901 aaaaccatca ccgatagtgc tcaagtactc ggtatc
//
LOCUS       DAZOYS010000275         3929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOYS010000275 DAZOYS010000000
VERSION     DAZOYS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.277"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(39..194)
                     /locus_tag="ACHUN9_003999"
     CDS             complement(39..194)
                     /locus_tag="ACHUN9_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0697255.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(500..919)
                     /locus_tag="ACHUN9_004000"
     CDS             complement(500..919)
                     /locus_tag="ACHUN9_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0697256.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            1016..1258
                     /locus_tag="ACHUN9_004001"
     CDS             1016..1258
                     /locus_tag="ACHUN9_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0697257.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            1255..1677
                     /locus_tag="ACHUN9_004002"
     CDS             1255..1677
                     /locus_tag="ACHUN9_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0697258.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1755..2554
                     /locus_tag="ACHUN9_004003"
                     /pseudo
     CDS             1755..2554
                     /locus_tag="ACHUN9_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2561..3307
                     /locus_tag="ACHUN9_004004"
     CDS             2561..3307
                     /locus_tag="ACHUN9_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0697259.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVERRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3322..3744
                     /locus_tag="ACHUN9_004005"
     CDS             3322..3744
                     /locus_tag="ACHUN9_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0697260.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3745..>3929
                     /locus_tag="ACHUN9_004006"
     CDS             3745..>3929
                     /locus_tag="ACHUN9_004006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697261.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT         1041 a          864 c         1041 g          983 t
ORIGIN      
        1 gtagagcgat aacgtgtttt aattctgtct cgttcatttt actcacctga atgtcttccc
       61 aaccaacgac gtgcgccagc ttcggtttta aacgttttgc ttttggtata cgtcatggcg
      121 gtgaatgtgc cgtcttgatt gggaaacacg ccacatacca gagattcgtt gttgccaaga
      181 tcgatagtat tcatgttgac cccatttccc cttaacgccg ggtggcggaa cgttttatct
      241 actgcgcttt gtatcaatca acaactgccg tcatgttcgt atgcctcagg ctggctactt
      301 agccctgttc agtgactgga taactcgagg tattgtcctg ccgttctctg gtggggcgtt
      361 gtttggatat gcttattaaa cacaatttgt tttcttgtgt caacacgcaa tgtgttttat
      421 ggtgagtgtc atatgatgat gctacaaaaa agcccgctga tagcgggctg attggcatat
      481 tactgtgata gcaagatcat tattccggtg ggggattatc tttaatcctg cctctcaaat
      541 atttttctac atactcatcg atttctttta gccggacttc aaatagctca atcattcgtt
      601 gttgttctga gcccggtagc tggttaaaca actcaagaag ttttcgttgg gattcattta
      661 accacaattc agaagattcc tgttctccaa agaggagctc aggaggagat atgccaagtg
      721 cctttcccaa tacgacagcg tcatgtactc caacatttct gctgcccgcc tcatagttac
      781 ctatacgcga ttgcgtccat ccgcagattt cagcaagttt tccttgagat aaaccaagct
      841 tctgcctgcg ctctttaaga cgcattgcaa ttttgtcatt gagcctacta gcggcaattt
      901 tttcgttttc tttttccatt gcatccttgt atcacgaatc gtgatttaca taaaacacaa
      961 aacagcttgg ctatataaca caaggtgtgt ttaaaattgt catcggaggt tttcaatgaa
     1021 caaaatttca acatatcgaa aacagcttgg gctgtctcaa agacaacttg ctgttcagtt
     1081 agggtggata caaagccgac tggcaaatta cgaagcaaat tttcgtaccc ctggactaga
     1141 ggagtgcaga aaaattgttt ctacccttaa tcggcttggc gctcattgtg gacttgacga
     1201 tgtattcccc ccagacggta agcatagcga aaacagcata ggagcggttg attcatgaaa
     1261 atcaggcatg agcacatcga atcagtgttg ttagccctgg cagccgaaaa agggcaggcg
     1321 tgggtcgcta acgcaattac tgaagaatat ctgcgccagg ggggcggcga attgcccctt
     1381 gtaccaggca aggactggaa caatcagcag aatatctatc accgttggtt gaaaggtgaa
     1441 acgaaagcgc aaagggaaaa aattcagaaa ctgatccctg cggttctggc aattcttccg
     1501 cgtgagctgc gtcaccgact ctgtatcttc gataccctgg aacgccgtgc attactggcg
     1561 gcgcaggaag cgttgagtac ggcaattgat gcgcatgatg atgctgtcca ggccgtttac
     1621 cggaaagcac atttcagcgg tggtgggtcg cccggcgatt ctgtcgtagt gcattgattg
     1681 aaattaatcg tgccggattg ttttgttcgg tatcagttaa atgtaacgct gcgagcgtta
     1741 caaggtgaaa acaaatggct tcaaactgga taaagctcga ggttattacg ccggataagc
     1801 cggaaatatt caggcttgct gagattctga atattgatcc agatgccgca ttagggaagg
     1861 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
     1921 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
     1981 cgttaattca ggttggatgg ctggtcgaaa atgacggtgg gctttctcta cctaactttg
     2041 aacgtcataa cggaaaaagc tctaaaaaaa aacgggcggt tacaaacgag cgagtaacaa
     2101 aaatacgcga actgaaacga aaaggtaacg ctgccagcgt tacacaaacg gatcaaaaag
     2161 cgttaccaga ggaagaggaa gaggaagagg aagaggaaga ggaagatcta aatactgatc
     2221 tccccctcgc caaaaacgag cgtctaaaaa attcgagccg gaggctattg agctgcccga
     2281 ttggttgccg gaaacactct ggcatgagtg ggtccggttc agacaggcat tgcgaaaacc
     2341 gattcgaacg gagcagggcg ctaacggggc gatacgggaa ctggaaaaat tccgtcagca
     2401 gggttttaca cctgagcagg tgattcgaca cagcatcgcc aatgaatacc agggcctgtt
     2461 cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag gttaacaccg tctcgttgcc
     2521 ggacagtgcg atcccgccag gcttcagggg gtaacggacc atgaaaaata ttgcgacagg
     2581 cggcgttctg gaacgcatcc gccgactgac cccgccacat gtaaccgccc cattcagaac
     2641 ggtagcggag tggcgcgagt ggcaacttgc tgaaggccag aaacgtagcg aggagatcaa
     2701 ccgcctgaat cgccagttgc gggtggaaaa aattctgaat cgctcaggca tccagccgtt
     2761 gcaccgtaaa tgctcgtttg cgaattacca ggtgcagaac gacggccagc gatacgcgtt
     2821 aagccaggcg aaatccatcg ccgatgaact gatgaccggg tgtacaaatt ttgcgttcag
     2881 cggaaaactt ggtaccggga agaatcactt agcggcagct atcgggaatc gcctgctgaa
     2941 agacggtcag acagtgattg tggttaccgt ggctgatgtt atgagcgccc tgcacgccag
     3001 ctatgacgac gggcagtcag gcgaaaaatt tttgcgggag ctgtgcgaag tggatctgct
     3061 ggttcttgat gaaattggca ttcagcgcga gacgaaaaac gagcaggtgg tgctgcacca
     3121 gattgttgag cgccggacag cgtcgatgcg cagcgtgggg atgctgacaa acctgaacta
     3181 tgaggtgatg aaaacattgc tcggcgagcg ggtgatggat cgcatggtca tgaacggcgg
     3241 gcgctgggtg aattttaact gggagagctg gcgtccgaat gttagccatt cgagggttgt
     3301 taagtagttt caggaggatt tatggcgaaa ccttttactc ccgaacagcg ggaagaactg
     3361 aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg tcacgattcg gcagttgtca
     3421 gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat gcatagaact ggtcgcaagt
     3481 ggtgatgtat ataattctgg ctacggctta ttcccgtctg aacaggctcg caaggactgg
     3541 caaagcgccc gcaaaaaact ctcgagagta aaggtgagga aaccggttgt tgttgatccg
     3601 gaccttatct ggtcattacc tgacggagaa atacgccgct acgacaggcg cctgaatata
     3661 atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg tactggcttt ctatcagggt
     3721 aattttcagg aggcgatact gtgaatgaaa ttagctatca ggcttcaatt accgctggca
     3781 ttcgcatcaa aggagaggag catggaaata aaaccagaag atgagttaag caatatcgtt
     3841 ttatttccgg taaaagagga tgaccctcgt aatcaggtta attttcttta tgagccatcg
     3901 gaaagaccat attgccatca cgcctctgt
//
LOCUS       DAZOYS010000276         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOYS010000276 DAZOYS010000000
VERSION     DAZOYS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.278"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUN9_004007"
     CDS             24..590
                     /locus_tag="ACHUN9_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0697262.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUN9_004008"
     CDS             594..1169
                     /locus_tag="ACHUN9_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0697263.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUN9_004009"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUN9_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0697264.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUN9_004010"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUN9_004010"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0697265.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUN9_004011"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUN9_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZOYS010000277         3888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOYS010000277 DAZOYS010000000
VERSION     DAZOYS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.279"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(16..336)
                     /gene="chbB"
                     /locus_tag="ACHUN9_004012"
     CDS             complement(16..336)
                     /gene="chbB"
                     /locus_tag="ACHUN9_004012"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0697266.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(635..973)
                     /gene="osmE"
                     /locus_tag="ACHUN9_004013"
     CDS             complement(635..973)
                     /gene="osmE"
                     /locus_tag="ACHUN9_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0697267.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1175..2002
                     /gene="nadE"
                     /locus_tag="ACHUN9_004014"
     CDS             1175..2002
                     /gene="nadE"
                     /locus_tag="ACHUN9_004014"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0697268.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2232..3119
                     /gene="cho"
                     /locus_tag="ACHUN9_004015"
     CDS             2232..3119
                     /gene="cho"
                     /locus_tag="ACHUN9_004015"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0697269.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3079..3654)
                     /gene="ves"
                     /locus_tag="ACHUN9_004016"
     CDS             complement(3079..3654)
                     /gene="ves"
                     /locus_tag="ACHUN9_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0697270.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          998 a          960 c          923 g         1007 t
ORIGIN      
        1 aaaatttaaa ataaattaat ttgctgcggc ttttttaatc gctgcaaccg cagccttaag
       61 cacgcctaaa ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt
      121 gggtaacaaa cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac
      181 atcggcattc tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg
      241 aacttcatat ttttctgcct gtgcgcgcat ttttgatacc agtaaagagg tagacatgcc
      301 cgcagaacaa aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat
      361 ctgtcagcca gacactccgc aagccttaac ctgcttccat gctctgggta acttgcgaaa
      421 ccaacatgac tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt
      481 tctttgacct tataaattgt ttctccttga cctgtggtta tgaccctctt cacatttcgg
      541 gcaaataatt cgcgaagata attaagaact caggccaaaa aaaaccggcg caatggccgg
      601 tttccgttgt tactcaagct ttcagacgaa ttgattactt cgcagcctgt ggatcagtgt
      661 cgtattcagc acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga
      721 caaagtaggt ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccac
      781 gagcatggat catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc
      841 ggctcatgcc ttttttgacg tctttcacca caggctgtac aaactggtct ttagtacgat
      901 cataagccgt acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca
      961 tattcttgtt catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat
     1021 tgggcgcttt ttcaagcccg cgaacgaaac ggctccgctt tcagaggatt cctgtatgac
     1081 gttttaacca ccattcagcc cgctgtcgct tgtcgtttca gtggcaacgg gttagcttta
     1141 aggaagtttt gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa
     1201 ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtatcgattt
     1261 tctgaaaagc tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg
     1321 cggtcaggac tccacgcttg ccggaaagct gtgccagatg gcgattaatg agctgcgcct
     1381 ggaaaccggc aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc
     1441 cgacgaacaa gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt
     1501 taatatcaag ggcgcggtat tggccagcga acaggcattg cgggaagcag acattgaact
     1561 gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat
     1621 tgcgggtatg accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg
     1681 attcttcact aaatatggtg acggcggtac agacattaat ccgctgtatc gtctcaacaa
     1741 acgtcagggt aaacagttac tggcggcatt aggttgcccg gaacaccttt ataagaaagc
     1801 gccaacggcc gatctggaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt
     1861 gacctatgac aatatcgacg actatctgga agggaaaaac gtacctgaac aggtcgccag
     1921 aacaatagag aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaccgtttt
     1981 cgatgatttc tggaaaaagt aataatttgc acataatggt ttgtgcgaaa aagagtaatt
     2041 tgttcacgcc ggatgcggcg tgaacgcctt attcgaccta caaaactatg caaattcaat
     2101 atattgcagg agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc
     2161 atcgacccgc ctctttttta atcgctcccc gcctgttaca ctggatagat aaccagcatt
     2221 cggagtcaac agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa
     2281 tttatgaata tcccgaacat ttacgttcat tccttaatga cttacccacc cgaccagggg
     2341 tgtatctgtt tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata
     2401 tccgcagtcg ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat
     2461 cccggcgaat tagctggatc tgtaccgcag gcgaaatcgg cgctctgctc cttgaagcgc
     2521 gattaatcaa agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct
     2581 gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt
     2641 cacgagcccc caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc
     2701 agaccatcgc tgatgaacaa aaactttgtt atggcctgct gggactggaa ccgttaagtc
     2761 gcggtcgtgc atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag
     2821 agagccatga ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgggtggtgt
     2881 gttggccttg gcaaggtgcg gtggcactga aagaacagca cccggaaatg actcaatatc
     2941 atattattca aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt
     3001 taattcggac acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc
     3061 tttccggtaa ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat
     3121 ttcactgaac aacagtttac cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa
     3181 ccagcacgcg ccctggtcgg tagtgagcaa tttatcgccc agttgccacg ccccgttaat
     3241 cacaaacacc accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac
     3301 tttcgcctta cagacatcca ggcgtgtcat gatgttgaaa tccatcgaca tctgcccttc
     3361 cgtcagtttc gcttttacca cctggtccgc tgcaaaggca aaaggctgta acggttttaa
     3421 ggtatggtta aagcggtctg cgctttcaag gagcatctcg ccgccttcca gcaacgtcac
     3481 tatcctttcc atgccgggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct
     3541 ggcacgccag taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc
     3601 ggcagcattt cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatactcgc
     3661 tcccggcccg ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc
     3721 gggtgattta cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa
     3781 aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag
     3841 ttttttggct gaaagattat tcagcagttg caggcatttt accttttg
//
LOCUS       DAZOYS010000278         3843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOYS010000278 DAZOYS010000000
VERSION     DAZOYS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.280"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(57..269)
                     /gene="cspA"
                     /locus_tag="ACHUN9_004017"
     CDS             complement(57..269)
                     /gene="cspA"
                     /locus_tag="ACHUN9_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0697271.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(550..840)
                     /locus_tag="ACHUN9_004018"
     CDS             complement(550..840)
                     /locus_tag="ACHUN9_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0697272.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1274..1984
                     /locus_tag="ACHUN9_004019"
     CDS             1274..1984
                     /locus_tag="ACHUN9_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0697273.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2034..3008)
                     /gene="ghrB"
                     /locus_tag="ACHUN9_004020"
     CDS             complement(2034..3008)
                     /gene="ghrB"
                     /locus_tag="ACHUN9_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0697274.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3112..3771)
                     /locus_tag="ACHUN9_004021"
     CDS             complement(3112..3771)
                     /locus_tag="ACHUN9_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0697275.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT          928 a          946 c          958 g         1011 t
ORIGIN      
        1 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
       61 aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
      121 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
      181 acatctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
      241 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
      301 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
      361 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
      421 caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt atttttcata
      481 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
      541 gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
      601 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
      661 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aaccctgtcc cttttcgtaa
      721 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
      781 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
      841 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
      901 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
      961 gcctgtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
     1021 taccactcct gttttgtaaa gatttcgaaa atctaacctt ctgcgaaggc gtaaatctct
     1081 ggctacaaaa aaggcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg
     1141 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     1201 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     1261 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     1321 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     1381 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1441 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1501 ttctcagcag gtgaatcagg cgatggattc tggtctgcgc ccggtcgtag acagcgttaa
     1561 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
     1621 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     1681 ctctgcgcta aaacagagcg atgacctgaa accggtattt gatcaggcat tcaccaaagt
     1741 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     1801 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     1861 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     1921 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     1981 ataaagagta aaagcccgag cgattatcgg gctttacttc acgcagtcgc ggcttagtcc
     2041 gcgacgtgag gattcacaca gttcttctca acctttcctt gtaacgcatc aattaaatta
     2101 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     2161 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg
     2221 aagacatcaa gcccggcggc gtgaatttcc cctttctgta atgctgcgat cagtgcattt
     2281 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     2341 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     2401 aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2461 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     2521 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     2581 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     2641 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     2701 acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     2761 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcatgcag tttcggcatt
     2821 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
     2881 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     2941 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     3001 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     3061 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     3121 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     3181 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     3241 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg
     3301 accccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctgtcag
     3361 caccattgcc acgccggtca gggtgttagc gcctgccggt ttcagggtcg cgctgctgct
     3421 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3481 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     3541 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     3601 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     3661 gatagcagat ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     3721 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     3781 ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc cgcgtgaaca
     3841 tga
//
LOCUS       DAZOYS010000279         3837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOYS010000279 DAZOYS010000000
VERSION     DAZOYS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.281"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..2259
                     /gene="faeD"
                     /locus_tag="ACHUN9_004022"
     CDS             <1..2259
                     /gene="faeD"
                     /locus_tag="ACHUN9_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HFR0697276.1"
                     /translation="SYAPGRYLVDLSLNGKEAGKQILDVTPQDSDELCLTEAWLAKAG
                     IYVSADYFREGYDATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDWDY
                     GTGAFRVNYNANANIGRNNTSTFGSADLKANIGHWVVNSSASASGGDSGDNSATINAF
                     TATRAIRALSADLTVGKTSTGSSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIANG
                     ASRVTLTQNGRLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQTQLFPLSVMA
                     GQLNPGQHEFSVAVGLSDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNRDWQGISAGA
                     VLGLSYLGAVSADGAYAMAEYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEEYE
                     DMSSFDPTELWSQSNHGRRPKDEWNAGISQSVGGLFSLSVSGWQRSYYPAQMTGRYRY
                     SDDNGLDTGITGTLSTQIKGVSLNLGWSGSRNSQGENNRSASASISVPFTLFERRYSS
                     STSVSTSKGGGTGFSTGVSGSLNDRFSYSLGGGRDSGGGVSSYLNASYSGDRAYLNGA
                     LNHSQSGGTSGSVSVSGSVLVVPMAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDGQT
                     DSDGNLVVPLNSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVKRY
                     LLQVKQRDGEFVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAARL
                     QDTEKLQEITCE"
     gene            2252..3034
                     /gene="faeE"
                     /locus_tag="ACHUN9_004023"
     CDS             2252..3034
                     /gene="faeE"
                     /locus_tag="ACHUN9_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HFR0697277.1"
                     /translation="MSKRNAVTTFFTNQVTKALGMTLALMMSCQSAVASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIAADNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKSAISSAQKSSDTEGNSSDKK"
     gene            3069..3560
                     /gene="faeF"
                     /locus_tag="ACHUN9_004024"
     CDS             3069..3560
                     /gene="faeF"
                     /locus_tag="ACHUN9_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HFR0697278.1"
                     /translation="MKKTMMVVALALSALSIQSTLAAEYSEKAQYLGVVNGQVVGNSV
                     VKVTRIPTDPVLYRSGDTTPLPDRLTIRNAESRAASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVAISARQQGEDMVITLPEAQKQIELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
BASE COUNT          929 a          888 c         1227 g          793 t
ORIGIN      
        1 agctatgcgc cgggacgtta tctggttgac ctgtccctga acgggaagga ggccgggaaa
       61 cagatactgg atgtgacacc acaggacagt gatgaactgt gcctgacaga ggcatggctg
      121 gcgaaggccg ggatttacgt cagtgcagat tactttcgtg agggatatga cgccacacgg
      181 cagtgttatg tgctgacaaa agcgccgtca gtgaaggtgg attttgatgt ttccacccag
      241 agtctggcgc tgtccattcc ccaaaagggg ctggtgaaga tgccggagaa cgtggactgg
      301 gattacggga ctggtgcatt tcgcgtgaac tataacgcga acgccaacat cggccggaat
      361 aacacctcga cctttggctc ggcagacctg aaggccaata tcgggcactg ggtggtgaat
      421 tcttctgcct cagccagcgg tggtgacagc ggggataact ccgccacgat aaacgcgttc
      481 acggccaccc gggccatccg cgcactgagt gcggacctga cggtcgggaa aacatccacc
      541 gggagcagtc tgctgggcag tacgggaacg tacggagtgt cgctgagccg gaacaacagc
      601 atgaagccgg gcaatctggg gtataccccg gtgttcagcg gcattgcgaa cggggcgtcg
      661 agggtgacac tgacacagaa cgggcggttg ctgcattcgg agatggtgcc ggcaggtccg
      721 ttctccatca cggatgtgcc gctgtacacc agtggggatg tgaccatgaa aatcaccggc
      781 gaggacggtc gtgagcagac tcagctcttc ccgttgtcgg tgatggccgg gcagttaaac
      841 ccgggacaac acgagttcag cgtggcagtc ggtttgtctg acgatgacag tgacctgaaa
      901 ggcggcgtgt ttgcggcgtc atacggttac ggtctggatg gactgacgct gcgcaccggg
      961 ggcgtgttta accgggactg gcagggtatt agcgccgggg ctgttctggg gctgagttat
     1021 ctgggggcag tgtctgctga cggggcttat gccatggcgg aataccgtga cggcagccgc
     1081 agcggaaata aggtgcagtt gtcctggagt aaacaactgg agatgacgaa caccggactg
     1141 cgggtgagct ggtcacggca gagtgaggaa tatgaggaca tgtcctcctt tgacccgaca
     1201 gagctgtggt cgcagtcaaa tcatggtcgc cggccgaagg atgaatggaa tgccggtatc
     1261 agtcagtcgg tgggggggct gttcagtctg tcggtgtccg gctggcagcg gagttattac
     1321 ccggcacaga tgactggccg ttatcggtac agcgatgaca atggtctgga cacagggatt
     1381 accggtaccc tgagcacaca gattaagggc gtcagtctga atctgggctg gtccggctca
     1441 cggaattcgc agggagagaa taacaggtcg gcgtcagcct ctatctcggt gccattcacg
     1501 ctgtttgagc gtcgctacag cagcagtaca tcggtgagta ccagtaaagg cggtggcacg
     1561 ggcttcagca ctggcgtgtc cggctcgctg aatgaccgtt tcagctatag cctgggcggc
     1621 ggtcgtgaca gcggtggtgg tgtgagtagt tatctaaacg cgtcgtacag tggtgaccgg
     1681 gcttatctga atggtgccct gaaccactca cagtccggcg gaaccagtgg ttctgtatca
     1741 gtcagcggtt cggtactggt cgtcccgatg gcgaaagaca ttatgttcag ccgcacgacc
     1801 ggggacaccg tggccgtggt gaacgtgaag gatacaccgg gtgtgaaggt gacgtccggt
     1861 gacggacaga ctgacagtga cggcaacctg gtggtaccgc tgaacagcta tgactggaac
     1921 acggtgacga ttgatgcggg cacactgccg ctgagcaccg aactgacgaa caccagtcag
     1981 aaggtggtgc cgacggacaa ggcggtggtc tggatgccgt ttgatgccct gaaagttaag
     2041 cgttacctgc tgcaggtgaa gcagcgtgac ggtgagtttg tgccaggggg aacctgggca
     2101 cgtgacagta agaacacacc gctgggcttt gtagctaaca atggtgtgct gatgattaac
     2161 acggtggatg cgccgggtga tatcacccta ggccagtgcc ggatacctgc ggccagactg
     2221 caggatactg agaaactaca ggagattacg tgtgagtaag cgtaacgcag taacgacgtt
     2281 tttcactaac caggtgacaa aagcactggg aatgacgctg gcgctgatga tgagctgtca
     2341 gagtgcggtg gcttccctgg cggcagacca gacccgctat atctttcgcg gggacaagga
     2401 tgcgctgacc atcacggtca ccaacaatga caaggaacgt acctttggtg gtcaggcatg
     2461 ggtggacaat atcgtggaga aagacacccg tccgactttt gtggtgacgc cgtccttctt
     2521 caaggtgaag ccgaatggtc agcagacact gcgtatcatc atggcctcgg accatctgcc
     2581 gaaggataaa gagtcggtgt actggctgaa cctgcaggat attccgccgg ctctggaggg
     2641 cagcggtatt gcagtggcgc tgcgcacgaa actgaaactg ttctatcgcc cgaaggcact
     2701 gcttgaaggt cgcaaggggg cagaagaagg catcagtctg cagagccgcc cggatggcag
     2761 gaccatgctg gtgaacacca cgccatacat ttttgcgatt ggcagcctgc tggacggaaa
     2821 cggaaagaaa attgccgcgg ataacgagac ggcgcagaaa ctgctgatgt tcatgccggg
     2881 tgatgaagta caggtgaagg gaaatgtggt gaaagtggac tctctgaatg attacggtga
     2941 actgcagaca tggacgatta accagaagaa atcagcaata tcgtctgcac agaagtcatc
     3001 tgatacagag ggtaattcat ctgataaaaa ataatcgccc tccggcatac ggaacagagg
     3061 ggtaataaat gaagaaaaca atgatggtag tcgccttggc cctgagtgca ctcagtattc
     3121 agtcaacact tgccgctgaa tacagcgaga aagcgcagta tctgggggtg gtgaatggcc
     3181 aggtggtggg taacagcgtg gtgaaggtaa cccgcatacc gacagatccg gtgctgtacc
     3241 ggagtggtga cacgacgcct cttcccgacc gcctgaccat ccgtaatgca gaatccagag
     3301 cagcatctgg tggcttggcg tatatcacgg tgaaacaggt gctgccggat aacggggaag
     3361 cccgcatcac cctgaagacg gccctgatgg ttgacggaaa gaaagtggca atcagcgcca
     3421 gacagcaggg tgaggatatg gtgataactt tgccggaagc acagaagcag attgaattaa
     3481 gaacggatgc accggcagag ctggaagtac ctgtcagtta ccggggaaat ctgcagatag
     3541 cgctgcaggt ggaggactga ggattcattt cctttataaa tgcagtacat cccgtgatac
     3601 cggagcgggt catattggta aagggattta ccgtctttta aaacgggaat aacgtaaaac
     3661 tattctgagt taaacactgt gtttaatgaa atgcggttat ttaaatgaat ccgcagggga
     3721 tggttttgcg gtaattccgg gaaaataagg attaccgatt cagtttatta tttatgaaac
     3781 gtcaaggtga ttattttatg aaaaagactc tgattgcact gactgtggct gcttctg
//
LOCUS       DAZOYS010000280         3833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOYS010000280 DAZOYS010000000
VERSION     DAZOYS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.282"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHUN9_004025"
     CDS             complement(<1..265)
                     /locus_tag="ACHUN9_004025"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0697279.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="ACHUN9_004026"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="ACHUN9_004026"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0697280.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="ACHUN9_004027"
                     /pseudo
     CDS             complement(725..997)
                     /locus_tag="ACHUN9_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHUN9_004028"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHUN9_004028"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0697281.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3733)
                     /locus_tag="ACHUN9_004029"
     CDS             complement(2693..3733)
                     /locus_tag="ACHUN9_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0697282.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         1000 a          932 c          916 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcatta cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tga
//
LOCUS       DAZOYS010000281         3798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOYS010000281 DAZOYS010000000
VERSION     DAZOYS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.283"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHUN9_004030"
     CDS             <1..82
                     /locus_tag="ACHUN9_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697283.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUN9_004031"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUN9_004031"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0697284.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHUN9_004032"
     CDS             1028..1486
                     /locus_tag="ACHUN9_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0697285.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUN9_004033"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUN9_004033"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0697286.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUN9_004034"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUN9_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0697287.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="ACHUN9_004035"
     CDS             2692..3369
                     /locus_tag="ACHUN9_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0697288.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUN9_004036"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUN9_004036"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0697289.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAZOYS010000282         3753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOYS010000282 DAZOYS010000000
VERSION     DAZOYS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.284"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUN9_004037"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUN9_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0697290.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUN9_004038"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUN9_004038"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0697291.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUN9_004039"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUN9_004039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0697292.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          857 a          983 c          910 g         1003 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gctgtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa tta
//
LOCUS       DAZOYS010000283         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOYS010000283 DAZOYS010000000
VERSION     DAZOYS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.285"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHUN9_004040"
     CDS             complement(<1..182)
                     /locus_tag="ACHUN9_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0697293.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHUN9_004041"
     CDS             426..683
                     /locus_tag="ACHUN9_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0697294.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUN9_004042"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHUN9_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0697295.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUN9_004043"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHUN9_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0697296.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUN9_004044"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHUN9_004044"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0697297.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="ACHUN9_004045"
     CDS             complement(3308..>3734)
                     /locus_tag="ACHUN9_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0697298.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAZOYS010000284         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOYS010000284 DAZOYS010000000
VERSION     DAZOYS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.286"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            224..1327
                     /gene="proB"
                     /locus_tag="ACHUN9_004046"
     CDS             224..1327
                     /gene="proB"
                     /locus_tag="ACHUN9_004046"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0697299.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1339..2592
                     /gene="proA"
                     /locus_tag="ACHUN9_004047"
     CDS             1339..2592
                     /gene="proA"
                     /locus_tag="ACHUN9_004047"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0697300.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2707..2782
                     /locus_tag="ACHUN9_004048"
     tRNA            2707..2782
                     /locus_tag="ACHUN9_004048"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2740..2742,aa:Thr,seq:cgt)
     gene            3388..3546
                     /locus_tag="ACHUN9_004049"
     CDS             3388..3546
                     /locus_tag="ACHUN9_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697301.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          951 a          879 c          993 g          911 t
ORIGIN      
        1 gtttttaacc tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt
       61 aatttattaa atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc
      121 aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc tgctaaaacg
      181 ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac
      241 gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc
      301 ccatattgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt
      361 tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc cggaactgcc
      421 agcgactatc gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact
      481 gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc
      541 tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga
      601 taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt
      661 cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt
      721 gctgctgacc gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga
      781 actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtgacagcgt
      841 ttcaggcctc ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg
      901 tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat
      961 ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa
     1021 acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc
     1081 cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa
     1141 tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggccgcgata tcgcccacgg
     1201 cgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat
     1261 tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac
     1321 ccgttaagga gctggccgat gctggaacaa atgggcattg ctgcgaagca agcctcgtat
     1381 aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa
     1441 ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc tgacgcgcga
     1501 gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa
     1561 ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc
     1621 gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggggtt
     1681 attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg
     1741 aaaaccggta atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca
     1801 acggtggcgg tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag
     1861 gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc
     1921 gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca
     1981 atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag
     2041 atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat
     2101 acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc
     2161 aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg
     2221 caggcaggcc ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg
     2281 tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt
     2341 gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa cgcccagcgt
     2401 tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac
     2461 ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt
     2521 ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc
     2581 attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac aaggccattg
     2641 acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct
     2701 ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt
     2761 tcgactccta ttatcggcac cattaagaaa attaataact tagcttaact tctctaagaa
     2821 attgtatctt ctgttatcac ttacaaacgc ctatacgttg ttatgggctt tggtagtgta
     2881 acacgtatgg gcgagtagtt cccaggctca acctcgtaac aagttccttt gaagctggaa
     2941 gacgtgccac cgtctctaaa ccttctacca aaactcttag caagatgttc cacacctggc
     3001 gcggtgtcca atgatgctac acgtcgtgag acgttgcaga ggataaacag gctaaatgat
     3061 gggaagagtt ttaagtgcaa tagtggatta aagcccacct cttcaaaacc taacttgatc
     3121 gggaaagaat caagctctag cacggtttcg tccaccggag tacaacgggt gcctgataag
     3181 agaagaactg ctaagacttc atagcttttg tgaaaaagcc ctttttagtg gatacgccta
     3241 cggcgcttat tttttggtat aaccatcaaa ggcgctttat ggctcctatt gtcttgaaaa
     3301 tgtatcttta cttgtattgt gttttatgcg tagtctccct gtgtagtatg taacttgtta
     3361 attttcatat gaatacagag gcacacgatg aaaaacattg cagcaatcaa acgtaataac
     3421 cgcaagattc acgctcgtaa gttcctgtct acgccagaag gaaaagcctg gctagagcgt
     3481 aaacagagag agaacgaaga aagaaaactc cttagtgagt tgaaatggct tagagaggat
     3541 ttctgaaaat ctccagaacg caacaggacg cattctaacg ctgttttcat tactcggtat
     3601 agggataggt gatgtgtgtt taatgcgtct gtgcgcttgt tttgtgcctt attttgcatt
     3661 ttcataatgc ggaagagaac acggaaaacc ttaaaattaa ttattggggt atttaaggct
     3721 attttcaggt gatt
//
LOCUS       DAZOYS010000285         3632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOYS010000285 DAZOYS010000000
VERSION     DAZOYS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.287"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(94..657)
                     /locus_tag="ACHUN9_004050"
     CDS             complement(94..657)
                     /locus_tag="ACHUN9_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0697302.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(761..979)
                     /locus_tag="ACHUN9_004051"
     CDS             complement(761..979)
                     /locus_tag="ACHUN9_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697303.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1138..1524)
                     /locus_tag="ACHUN9_004052"
     CDS             complement(1138..1524)
                     /locus_tag="ACHUN9_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0697304.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1514..1885)
                     /locus_tag="ACHUN9_004053"
     CDS             complement(1514..1885)
                     /locus_tag="ACHUN9_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0697305.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1885..2142)
                     /locus_tag="ACHUN9_004054"
     CDS             complement(1885..2142)
                     /locus_tag="ACHUN9_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697306.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2139..3530)
                     /locus_tag="ACHUN9_004055"
     CDS             complement(2139..3530)
                     /locus_tag="ACHUN9_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0697307.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3527..>3632)
                     /locus_tag="ACHUN9_004056"
     CDS             complement(3527..>3632)
                     /locus_tag="ACHUN9_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0697308.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1016 c          812 g          954 t
ORIGIN      
        1 ccgctcatcg gcgggtttaa gctgtgtggc gtagtaacca ctcttaacag gatattcaac
       61 tttttacgat cgtaaagttt tcggaaaaaa tttttaaaac cgcatcaacc gctccaccag
      121 ttgctcttta cgggcaacga tccacccgtg ttgctccaga taaaatttaa accgttccag
      181 agtacatacc atcgcatcgg cgggtacctt ttccgtgaac tcaacctgac cgtgtttatc
      241 gaagtggctc agtaatgcgc atccatcatt ttcgatagat gtgttttgtg ccgctggtgg
      301 ctgtttctgg ttgaaataac agtcttccag tttttcgaac acttcccacg cctgatcggt
      361 ttcgagcatt ttggcgtgac gggctgcgcc acgttctgtc cagaggatga gggagcgaac
      421 gttacgggca attttcacag aaactcttaa agagttttcg ttcttcaact ctcgtaacgc
      481 atcaccttct atcaggaaaa aatgtttacc ttctataaac cgttctttgt ttcgattgaa
      541 attgtttgtc aaacgctggc gctctgttcc gtacaaatca gccagcagct ctgttgtcat
      601 gacaggaaga tggttatgcg taagtgacgg gagtttttca atagacgtgc gattcataga
      661 catgtccttt cgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc
      721 gtcgaaagtc ggcgggcgta tttccattgc tggtattgta ttagccgcac acccggtcat
      781 aaaccaagaa ttctggacac aaaaaaacca catggctatc gggtgtggat accgctttcg
      841 aggtgttttg agcaccgcgg ggaaatgtaa actgcaaacc tgaaactgtc aacaccgtca
      901 ggttccaatc atcgggtggt gagacgcaca gggttggaac taccgggaaa ccgaccggcg
      961 agcttttcag ctcccccatg cgccccacca taattcagat gtgcgcgtgc atacgacaat
     1021 aaaaaacacg ctcgcggcgt gtgtctgtcg cggtctctat ccggggttcc aatcccgacg
     1081 ccagattttg ctggcgcgtg aggaatatag ccccggacaa tgtgtctggt caagcaccta
     1141 cataattcgt tctacgtatc tgtccatctc cagccggata tcaagcatca tcaacatgcc
     1201 atcaataacc ccttccgctt tctgcaggcg cttgccaata caggtatccg aacacccatg
     1261 tttccgtgcc agactcataa aagtcattcc acctacgtaa taatccacca gcaaatcgtg
     1321 caaatcctga tttttcttgt tcaaccgggc catacagcca caaattatca ttgcatcatc
     1381 atcagaacac tgagggcgtg atttcacttt cgacggaatt aaccctttaa aaccagcagc
     1441 gatcgacgcc caggatacat cttcatgagt gtttgcagcc caagcccccc accgttccat
     1501 aacctgctga atatcacgcg ccatcgttat cacctgtgat ttcataaatc ttcacgccca
     1561 gtcgcccacc aggaacgagc tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt
     1621 ctatgagtag tccggcatgc gtcagcgcat ccagtggtgc tttcaggata ttgtccaggt
     1681 cacgacggcg cttatccggt ggctctgcaa taatctttat cgccagcctt ccggacaggt
     1741 ttaatttcag ccgctgctgg cgaacaatta gcgtcacatc acggcgataa cgctcaccaa
     1801 cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtattcacc gtcggcggat
     1861 aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc tgcaccattt tttcaaactt
     1921 tccaactttg gtttccagct ctgccacaca atcaacgagc tcatccaccg ctttttgtgc
     1981 gcgatgtttc gcctgcatca gttccctgag cgctggcacc atatcccgac gaatggcatc
     2041 ttttgttaca cctgtttttt ccagttgttc cgcctgtcgc aacatttcct gcgcgtgttt
     2101 acgcaattgt tcagaggtaa aagtcattgt ctggttgttc aaaagaaacg ctccatctta
     2161 ctgctgtcgg tacgtgtatt gctgtatctg cgcggctggg gctgccgcat tggggtggaa
     2221 agaacctgtg cgctttcctg gtccacaggc agaaaatgtc cgttataaaa acgccggtaa
     2281 atcgtcccca gagaaccgtt acgttgtttc gtgatattaa tttctgctat gcctgtagcc
     2341 tgtgtatccg ggttgtacac ttcatccctg taaagcatca gaatgatgtc tgcatccgcc
     2401 tctatttctc cggaattttt caggtctgag ttcatgggac gtttattggg tctggactcc
     2461 acaccgcggg agagctggct cagcgcaatc aacggaaaac caccggattt tgccaggcct
     2521 ttaagcccct ttgagatttc acccacggca aggtcatgac gccccgtggt tcgggttttt
     2581 atcagcccga gataatcaac caccaccagc gccgtttccg gatgtttaat cagatggtgt
     2641 ttcgttgttg cgcatatctc atcaatggtc aggttcgcct ggtccaccat ccagatattg
     2701 cgcccggtca tccgccccac cccttgtgag aaacgcgccc agtcttcatc ttcaaagtga
     2761 gccacagatt tcaggcgtga tactggcatc cctccagccg cagacaccat acgttcacca
     2821 atctggatgt tcgccatctc catggtgaac agaagcacac catgcccctg ctcagtcacc
     2881 ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg aaggacgggc ggcaatgaat
     2941 accaggtctc cgggctccat accgcctgtt tttgcgtcca gttcatcaat accggtcatc
     3001 aacgtcctgg atttctccag cccctgattc cggcattcaa cacgctcaac cacttccgga
     3061 agcacatcat caatatgtac cggctgaatg acgccctttc ctgtcgacag tgtgaccatc
     3121 atgttctgcg catccttcag ggcatcttca gctgcttcac aggtatgcgc atcacgtaat
     3181 ttctgcagcg cctcattcag tgttttttct gcatcgcgca gtgcggcatt gcgccgcaac
     3241 gctgcaacat agtgctccag tgaagacttc acccaggttt tacgcccggt atcagtaatc
     3301 accggggcaa gttccggcat ctcattacac aacagcacgg ggtcaatcac tcctgaaaca
     3361 cgggcctgtc tgcagatgcc tgtgtagata tcccgatacg ctcgtacaga aaaaatgtcc
     3421 gccggtagtg tgatcagaat atccatcact tcatgatctg ccccacgcag aaagaatgcg
     3481 ccaatgacag cgccttccag gtcatcatta cgccagactg gtgttgtcat gcagccacac
     3541 ctctgataca agaacggtag ctgggccagt tgaacgacaa ccagttgcgc cccccgtctg
     3601 tgatcctgtc ggcaatgcgg gggctgatga ac
//
LOCUS       DAZOYS010000286         3604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOYS010000286 DAZOYS010000000
VERSION     DAZOYS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.288"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            150..446
                     /locus_tag="ACHUN9_004057"
     CDS             150..446
                     /locus_tag="ACHUN9_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697309.1"
                     /translation="MAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQTYTVSVP
                     NMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKWTA"
     gene            448..1104
                     /locus_tag="ACHUN9_004058"
     CDS             448..1104
                     /locus_tag="ACHUN9_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697310.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
     gene            1179..1451
                     /locus_tag="ACHUN9_004059"
     CDS             1179..1451
                     /locus_tag="ACHUN9_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR0697311.1"
                     /translation="MDIGAILALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAV
                     LESSISRIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(1490..1939)
                     /locus_tag="ACHUN9_004060"
     CDS             complement(1490..1939)
                     /locus_tag="ACHUN9_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR0697312.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIVYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            1976..2410
                     /locus_tag="ACHUN9_004061"
     CDS             1976..2410
                     /locus_tag="ACHUN9_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697313.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            2420..2920
                     /locus_tag="ACHUN9_004062"
     CDS             2420..2920
                     /locus_tag="ACHUN9_004062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697314.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            2996..3307
                     /locus_tag="ACHUN9_004063"
     CDS             2996..3307
                     /locus_tag="ACHUN9_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697315.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            3304..3597
                     /locus_tag="ACHUN9_004064"
     CDS             3304..3597
                     /locus_tag="ACHUN9_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697316.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
BASE COUNT         1182 a          601 c          770 g         1051 t
ORIGIN      
        1 tcagggatta ttaactgtaa cctcatttca aaaggataca taaatgaaat ctaatgaacg
       61 tactttcaat agtaccagta ctaacccatt cagtattagc ggaaaagaaa caattggaat
      121 gaagaaaagg atttacagga gtaagagcta tggcacaggg attacaatgt tggaatgcag
      181 cgggtgtatt agtagtagat ctgactgatt ataacatacg ttttatgggt acttatactg
      241 tcaaggctac ttctgcacag acctatactg tatcagtacc aaatatgaaa actacaggct
      301 ggtttgtaca ctatgccccc gccagtgata cctttaatga gtggtcagcc ttttgtaata
      361 atgggtcatt tactgcggtt tatttgccaa catatgcacc gttagctggt aactacactt
      421 ttaacgtata taagtggacg gcgtaagatg gcaggtttcg aggtatataa ttcagcgggt
      481 gcactgacta tagatagtac taacaagtcg ataatgacgg gtgcagtaaa ggccatggga
      541 aatctaactg atacgggata ctacacaggc tttacctgtg ccttcggtaa tggtggatcg
      601 ctcggtttcg ttatgccatc agtagtagct aacaggaaca caacacagta ctggttccag
      661 atacagaaag acggggcatg gtgtttccct ggtgcctata tgttccagcc tggcatgggc
      721 aggttcatga caagtagtca cacggctacc cccacatctg gttttcttga tgtgttcagt
      781 gaagaaggta ctttaatctg gtctgctgca tcagcggcaa caatgccacg tatcagggga
      841 tttttgacag cacctgcggc taccgatctc agtactgcca ttacggtaac gtctccagta
      901 gctgatccct ggttctgctg gtcacaatgt ccggggaaca tatcagatga tggtacagta
      961 attgggtact ctggtctggt tatcaggcgt aattccagta cttcattcag tctacagtac
     1021 gtttcaaaga accagaaaac ctaccgccag gcaatgggga ataatggtat ccagatagca
     1081 ttggcgacat ttacagggta ttaatacaaa aacaataaca ataacaataa caataacaat
     1141 aacaataaca ataacaataa caacaaaggg atttcagtat ggatatcggt gctattttag
     1201 ctcttgttat ttctggatgc gtattccttt atactatctt tagagataat acaaaggata
     1261 cgaatgatct attaactcgc gttgctactc ttgaaactac cattgctgta ctggaaagta
     1321 gtatttcccg tattgaggat gatcaggata agatgagaga tacactaagt aaactggaaa
     1381 cacaaatcca tgaacttgat gttaagattg aaaggattat tgcaattctt cagcagaaac
     1441 aacgaccata aaaaaaggca tagtatgtca ttcgtactat gccttttctt tatttgtttt
     1501 taagtactgc aatcatggaa ttcacacggt ttggtgtctg cctgtaccat agagaacact
     1561 tagcctgttt aattgcttca aggtaatcat gtgctttcag ggctgctaac atcttacgga
     1621 atttcttcac cccgccaata cctaactgga aggtcatgat aataataaaa tcattccagt
     1681 catcgggaag atttaaccct aatgtctgta cctgcataat tgttttggct aaatcgcgag
     1741 atagtaactt gtctgcttcg ctttcagtaa tgccagtact aaaatcttct cccgcctgaa
     1801 ttaagtgacc atatacaata gtactacatc ctaaactatc agcatatgga tagaatttcc
     1861 catttctgaa gtacttcaaa tttgcctgat actctttagt accttcatat tcttttaatc
     1921 gtgtttttaa atcgctcatt tactaaatac ctataatgaa tttataggaa tatttatgat
     1981 gagtgaatgg aaatactcgg atgattggag cgaggaggaa atgttatccg gtgaatatgc
     2041 tggtttcgtt tatatctttc agtttgagga tggttctaat tatgtcggtg ctaagcaaat
     2101 ctatcagcgg gttaaggatg cgagaaagct aaagcccact tcaaaggaaa atggctggcg
     2161 tgagtacctc agtagttcca atgtcgttaa aaacaaaatt gaaaatggtg aacagtactc
     2221 aaaaacgatt ctatggtgct tcgcaactat gcgagaggta atgattgtag aggcaatact
     2281 gatccttcat cagatgctta aaccggaatg tttgaatctg gcaatgatga gtaagattag
     2341 atcaccaaat gcaaaggata aaaaaagact actcggaata gtacaggaac tattggagta
     2401 cttgaattga ggtaaatata tggcaagtgg aataaatggg attggtagta taaggaacta
     2461 tatacaaacg cagggagtta gattaaataa tcaattccag aatgaaataa ttaaacgttc
     2521 caagctacta tcccaaagaa tgcagggtga tttgaataat agcgttgacc gtggggcagt
     2581 gggctttact caacgttcca tccttttctt ctatagaaag gttgggagaa attcagttac
     2641 atcgaccatc atggttaaag atattcaggc taagtacctt tatcaagtac tggtacagcc
     2701 aaaggcaatt gataagttta tacctacttc atctacaaga ttaactaagc agggcaacat
     2761 ttcaggactg aaaaagaatc ttttaagtga acgatataag gtagtgaagg gtaagaatgg
     2821 taaagaacga ttaattgata ccgcaaagaa agataccaag aagaaaacca aacgtgtaat
     2881 tggcttacgt gagaagaaac aacgtcgcct tatctattga cttctataag gaagccaatg
     2941 atggtgtaag ggtaattatg agcggtattc agggaacatt caggataagt agacaatgaa
     3001 ttttgagcaa cattatggtg atttaataag caagattaag ctaaatggca tgacaccaga
     3061 tggtaatagt atgccaatta acaaatgttt tcttgataag aaatttcaaa aggtcgttaa
     3121 gaaaagtaat tatcagattg atagctatat gaacgttatt tatgagaaga agaaagattt
     3181 caaagttggt gaagtacttg aatgggaatt acagggtgaa aagatgcctg tatttctaat
     3241 tgagagtaaa aagctctttg tgaagggtaa acatttttgg gtatatgccg tggggattat
     3301 tgaatgatta gtatattgat tgagtttctg aagtcaggat tggatttttt cattaagaag
     3361 aaaactattg aacaggaagt aaagaaaacg aatgtagaag ggcagattga aacgaataaa
     3421 gaagagattg aaaaggtaac attccactgg cgtaatgccc tcggttttgt actaaccctt
     3481 atcattctct ataactggat catagtacca gtacttgatg cttttggaat tattgtgatc
     3541 caagttccat taggacaact tttgcaagtg ctattgatta tggttggtgg aagctgaaag
     3601 attt
//
LOCUS       DAZOYS010000287         3559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOYS010000287 DAZOYS010000000
VERSION     DAZOYS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.289"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHUN9_004065"
     CDS             129..1376
                     /locus_tag="ACHUN9_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0697317.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUN9_004066"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUN9_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0697318.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHUN9_004067"
     CDS             complement(2228..2572)
                     /locus_tag="ACHUN9_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697319.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHUN9_004068"
     CDS             2814..3416
                     /locus_tag="ACHUN9_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697320.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAZOYS010000288         3546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOYS010000288 DAZOYS010000000
VERSION     DAZOYS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.290"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUN9_004069"
     CDS             complement(<1..143)
                     /locus_tag="ACHUN9_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0697321.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHUN9_004070"
     CDS             complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHUN9_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0697322.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHUN9_004071"
     CDS             complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHUN9_004071"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR0697323.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHUN9_004072"
     CDS             complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHUN9_004072"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0697324.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          926 a          982 c          784 g          854 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta
      961 cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca
     1021 tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg
     1081 gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt
     1141 gtacgctcgc cggaaagata aggcagaaac caaactggct cgacactttc atcagcctgt
     1201 tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc
     1261 agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg
     1321 caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag
     1381 acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact
     1441 gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct
     1501 ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc
     1561 tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca
     1621 tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc
     1681 agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca
     1741 tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta
     1801 atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg
     1861 tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca
     1921 tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga
     1981 gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt
     2041 ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaat caaccacctc accctgctcg
     2101 ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc
     2161 gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta
     2221 gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac
     2281 tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca
     2341 tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga
     2401 tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca
     2461 tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac
     2521 gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat
     2581 acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt
     2641 gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta
     2701 tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa
     2761 tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat
     2821 attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga
     2881 ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac
     2941 gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt
     3001 caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga
     3061 agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt
     3121 tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa
     3181 ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca
     3241 catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg
     3301 ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc
     3361 ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac
     3421 gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg
     3481 ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg
     3541 cttgca
//
LOCUS       DAZOYS010000289         3546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOYS010000289 DAZOYS010000000
VERSION     DAZOYS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.291"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHUN9_004073"
     CDS             complement(37..1434)
                     /gene="ccp"
                     /locus_tag="ACHUN9_004073"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0697325.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1839..3488
                     /locus_tag="ACHUN9_004074"
     CDS             1839..3488
                     /locus_tag="ACHUN9_004074"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0697326.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          891 a          924 c          850 g          881 t
ORIGIN      
        1 atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg
       61 cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg
      121 cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac
      181 gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg
      241 acgcaattta tcacgctctt ctttagtaac attcatacga ccaatatccg ccgccgtaat
      301 ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc caccgagaat
      361 aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg
      421 ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt
      481 aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg
      541 cggatagacg ccgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat
      601 ttcgtcccaa gatttcgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc
      661 ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac
      721 cgtcggcgcg ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc
      781 gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa
      841 acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt
      901 ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta
      961 atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac
     1021 cttacccgcc cagtgaagcg cggtataacg cgttggcggc atagtttcat actgcatcac
     1081 ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg
     1141 cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt
     1201 cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca
     1261 tcctttttcg cggagaaaac ctaaaacctt attattttca ctgacagcgg atgcctgaac
     1321 atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata
     1381 gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct
     1441 tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc
     1501 tgcgagacaa agccatcata aaaaacagtg agatacgctt tttgatagga atcaatcaat
     1561 tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca
     1621 tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc
     1681 cgtattttta taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg
     1741 tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag
     1801 cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa
     1861 accctaatcc agacgaactg atgatcgaag tcgatctctg ctatgagctg gacccgtatg
     1921 aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc
     1981 ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt
     2041 cggcgaaaat tttcccggac agtaaaacct ttcccgactg cgcacctaaa atggacccgc
     2101 tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt
     2161 ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc
     2221 aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg
     2281 atcacattcc gtggtcttct ctactggcgc tgccgcagtc atatattgtc ccaggcggcc
     2341 gttttagcga aacctactat tgggactcct atttcaccat gctggggctg gcggaaagtg
     2401 gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactatg
     2461 gtcacatccc caacggcaac cgcacctatt atttgagccg atcgcaacca ccggtttttg
     2521 cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc
     2581 accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg
     2641 cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc
     2701 gcgacacgcc gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc
     2761 gcccgcccaa cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact
     2821 cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc
     2881 ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc
     2941 tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg
     3001 cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac
     3061 gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga
     3121 accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg
     3181 ggattctggc aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac
     3241 cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg
     3301 aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga
     3361 tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt
     3421 tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg
     3481 aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc
     3541 aggagg
//
LOCUS       DAZOYS010000290         3393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOYS010000290 DAZOYS010000000
VERSION     DAZOYS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.292"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUN9_004075"
     CDS             complement(<1..312)
                     /locus_tag="ACHUN9_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0697327.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUN9_004076"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUN9_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0697328.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPTVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUN9_004077"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUN9_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0697329.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          919 a          880 c          738 g          856 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgtgggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgt
//
LOCUS       DAZOYS010000291         3369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOYS010000291 DAZOYS010000000
VERSION     DAZOYS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.293"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUN9_004078"
     CDS             <1..784
                     /locus_tag="ACHUN9_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0697330.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHUN9_004079"
     CDS             781..1686
                     /locus_tag="ACHUN9_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0697331.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHUN9_004080"
     CDS             1683..2753
                     /locus_tag="ACHUN9_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR0697332.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHUN9_004081"
     CDS             2990..3343
                     /locus_tag="ACHUN9_004081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697333.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
BASE COUNT          889 a          777 c          930 g          773 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctca
//
LOCUS       DAZOYS010000292         3329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOYS010000292 DAZOYS010000000
VERSION     DAZOYS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.294"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..537
                     /gene="lpfB-O113"
                     /locus_tag="ACHUN9_004082"
     CDS             <1..537
                     /gene="lpfB-O113"
                     /locus_tag="ACHUN9_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0697334.1"
                     /translation="IPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHT
                     KGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAP
                     KVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMT
                     WRFINDYGAVSDPIKMTL"
     gene            562..3084
                     /gene="lpfC-O113"
                     /locus_tag="ACHUN9_004083"
     CDS             562..3084
                     /gene="lpfC-O113"
                     /locus_tag="ACHUN9_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0697335.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3095..>3329
                     /locus_tag="ACHUN9_004084"
     CDS             3095..>3329
                     /locus_tag="ACHUN9_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697336.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGN"
BASE COUNT          905 a          728 c          815 g          881 t
ORIGIN      
        1 ataccttatc tcatccaggc ttgggtagat ccatttaata aagaggataa aagcaaagcg
       61 ccgtttaccg ttattccgcc agtatcacga ttagaacctt cgcaagaaaa aatattacgc
      121 attattcaca ccaaaggtgt gtcattgcct gatgatcgcg agtcagtctt ctggctgaat
      181 attaaaaata ttccgccatc agcgagcaac aaagcgacga actcgctgga aatagcagta
      241 aaaacgcgta tcaagctgtt ctggcgacca gcaaatatcc gccttattcc cgaagatgct
      301 gcgccgaaag tgaaatggcg ccgggaaggg cgaaatctga tagcagagaa tcccaaccca
      361 attcatatca gtgtgatgga tgtcatcgtt gatggccatg atgtgcccct caatatgatc
      421 cgtccttttg aaacgctcac gttaccttta cctgctaatt ctgcgggagg ccaaatgacc
      481 tggcgattta ttaatgatta cggcgcagtc agtgacccga taaaaatgac gctgtaagca
      541 agggataagg ataggcgtat tatgatgacg accagaatag tggttggcct cacggcaggg
      601 acgtgtctga ttttctcgca aaacctgatg gccgaggtca gtgtattcaa tccggcgctt
      661 ctggaaatcg accatcaatc cggagtcgat attcgccagt ttaatcgggc aaacctgatg
      721 cccccaggtg tttatagcgt tgatattttt atcaacggta aaatgtttga acgtcaggat
      781 gtgacatttg ttcaggataa tccagatgct gatctgcacg cttgctttat tgccattaaa
      841 aaaacactgt cctcctttgg cataaaagtt gatgcgctca aatcgttcaa tgatgtggat
      901 gagacggttt gcctcgatcc tgctccacgt attgaaggct catcctggca gtttgacagt
      961 gataaattgc agctgaatat atccattccc caaatctaca tggacgcgat ggcttatgat
     1021 tacatcagcc ccacgcgttg ggatgagggg attaacgcgc tcaccatcaa ctacgatttt
     1081 tctggttcac atacactacg ttcagattat ggttcacaag agacagatac cagttatctc
     1141 aatctgcgca atggactgaa tattggaccg tggcggctac gtaattacag tactttaaac
     1201 accagcgatg gccgtgcgga atacaactcc attagtacct ggatacagcg cgatattgcc
     1261 gcgttaagaa gccagattat gattggtgat acgtggacgg cgagcgatat tttcgacagt
     1321 acgcaaattc gcggcgcgcg tttgtatact gataacgata tgctacccgc cagccagaat
     1381 ggctttgctc ctgtggttcg tgggattgca aagtccaacg ccaccgtcat cattcggcag
     1441 aatggctacg tgatttatca gtctgccgtt ccacaaggtg cttttgagat caccgatctc
     1501 aacaccgcca gtacaggtgg cgatttggac gtaaccatca aagaagaaga cggtagcgaa
     1561 caacgattca cccaacctta tgcttcatta gcgattctta aacgtgaagg tcagacagat
     1621 gttgatgtca gcgtgggtga attgcgcgat gaagacggat ttacaccgga cgtccttcag
     1681 gcgcaaatac ttcatggttt ttcccacggg atcactttat atggaggtat gcaggctgct
     1741 gaaaattatg gttctgcagc tctgggtgtc ggtaaagatc ttggcgcttt gggcgcaatt
     1801 tctttcgatg tgacacatgc tcgtgcgaat tttagccatg atgatacaga aacgggtcag
     1861 tcatatcgct ttctctattc aaaacgattt gacgacacag acactagctt gcgcttggtt
     1921 ggctatcgtt actccaccga gggctactat accctcaatg agtgggcatc gcggcgcaac
     1981 agccctgaag acttttggga aacaggtaac cgacgtagtt gcgtggaggg gacgctaacg
     2041 cagtcgttgg ggagagatta tggcaattta tacctgacat taagccggca acaatactgg
     2101 cataccgatg atgtcgaacg attaatgcaa tttggctaca gcagtagctg gaagcgtctc
     2161 tcgtggaacg tctcctggag ttattccaat actgccagac aggggacggg gaacaaccat
     2221 gccagtgata acaccagtga gcagatctac atgctctctt tatctgttcc tttattgggc
     2281 tggtggggta atagttacgc cacctattct gtttcgcaaa acgataattc cggtagctca
     2341 catcaactcg gactcagcgg tacggcgctg gaaagaaata acctttcatg gaatttaatg
     2401 cagtcctata acagtcatga tgatgaggtt ggcggtaata tgtccctgac ctatgatggc
     2461 tcttatggca cggtgaacgg cagctataac tacagccaaa attcccagag gctgaattat
     2521 ggtatcagag ggggaattct ggcacacagc gaaggggtaa cgttaagtca ggagttaggt
     2581 gaaactattg ctcttgttaa agcacctggg gccgccgggt tagaaataga taatatgcgc
     2641 ggtgctgcga cggactggcg cggctatacg gtcaagacac agctaaaccc ttatgatgaa
     2701 aatcgggtag caatcagcga taactatttc tcgaagtcga atatagaact tgataatacc
     2761 gtcgttacga tggttcccac gcgtggtgca gtggttaaag cggagtttgt gactcatgtg
     2821 ggttatcgcg ttctcttcag agtgttaaat gcaaatggta aaccggtacc ttttggagcc
     2881 attgctgcga tacaagatgc aagtttggca gattcaggaa ttgtcggtga ccgtggcgaa
     2941 ctttatcttt ctggtctacc agaaaaagga caggttacgt tatcctgggg agaaaacgcc
     3001 tcaacaaaat gcatcttcaa ttattcactt tcgacaccag aaagtgagag cggattaatt
     3061 gaacagggtg tgacatgtca ttaaaaggat ttttatgaac aagtatatca aacagtggtg
     3121 ctttgctgtg tttatgctct cgttaagtag tgtagcactt gcagctccta aaggtatctg
     3181 tacccctgat aatggcgtat ttcaccgtac gcttgatttc tcgggatatc ttattaccgc
     3241 gaacgaaaat aaggttggaa cgacatttaa taccactgta actaatggat cctcatatcc
     3301 tggacgctgt cattgtgata ctggtaatg
//
LOCUS       DAZOYS010000293         3329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOYS010000293 DAZOYS010000000
VERSION     DAZOYS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.295"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..844)
                     /gene="adrA"
                     /locus_tag="ACHUN9_004085"
                     /gene_synonym="dgcC"
     CDS             complement(<1..844)
                     /gene="adrA"
                     /locus_tag="ACHUN9_004085"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0697337.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(946..1266)
                     /gene="psiF"
                     /locus_tag="ACHUN9_004086"
     CDS             complement(946..1266)
                     /gene="psiF"
                     /locus_tag="ACHUN9_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0697338.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="ACHUN9_004087"
     CDS             complement(1385..2800)
                     /gene="phoA"
                     /locus_tag="ACHUN9_004087"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0697339.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="ACHUN9_004088"
     CDS             complement(2901..3161)
                     /gene="iraP"
                     /locus_tag="ACHUN9_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0697340.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          792 a          879 c          783 g          875 t
ORIGIN      
        1 taccgcgcag ggtaatttgt aactgtcggg taagcgccac aatcgcttca tcgcccacat
       61 catgacccca ggtatcgttg atgctcttga aatggtcgat atcgataatc agtaacgttg
      121 catcgcgatt atgccgccga cagttatcaa attcattgcg taacatagtt tcccaatgac
      181 gtcggttata cacgcccgtc atgccgtcgc gagtactcat gacctgcaac ctgcgtttat
      241 gttccgccag tttggttgcc gtctggtagc tgacccagcc aaacagcaga ggataaacga
      301 caataatggg aagggagagc caccattcca gcggcgcact attgaacgac acggtaatgc
      361 ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa caccagaccc gcgacaaaca
      421 gacgggggcc gcctgccccc atcaaattca gacacataat catcaacata gcggtggaag
      481 gcagcacgtt tacgcccatt acgcctaccc acattcccgc taatactgca tcggttttta
      541 agttgtaaat ttcccggcta agcggatcga cggccctgct cgctatctgc caggctaaat
      601 gcggccagac gaacgcccag ccgaccaaca ccagccacca ccagcccggc ggcgggtgcg
      661 aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag gccaaccgca cggggtagtc
      721 tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc gttttgagga gttaaaggag
      781 gttcctcccc atgcgccgcc gcttttttga aaaagttttc atcattcatt atttttggga
      841 acattcagaa acaattttcc caaattatag agacggggct aaaggcagaa gtatgaaatt
      901 tcgggtgagc cactttcgta gctcacccgt tagcactatc aggtatcagg cggctttctt
      961 gaggcaggca ctcataaact tattacgatc atcacctttc agagattgtt gcgttgcttg
     1021 attattacat tcgcgcatct tttgctgctg tggcgtcaaa cttttttcac caggcgcaga
     1081 cttgctgttc ttcaggcaat cactcatgta ggtcttacga gcatcccctt tcaacgcctg
     1141 cgccgtcgcc tgctgattac aggaggtcat acgctgttgt tgtggggtta aggttctctc
     1201 ggcagcgccg acggtggtta aaaaaaccag accaaaaagc aaggtaacca gtaatgttat
     1261 tttcatagca ccatccctct tcatgtttta accatgagcg tttgcgcccg tgatctgcca
     1321 ttaagtctgg ttgctaacag caaaaaaacc acccggcagc gaaaattcac tgccgggcgc
     1381 ggttttattt cagccccaga gcggctttca tggtgtagaa gagatcggtc tggtcggtca
     1441 gtccaacaac attggcggca tgcggaccat acgccgcaat acgcaactga ctgccggtat
     1501 gttcttgtga atcctcttcg gaattcccgt aactcatcac catcactgcg ccatctttag
     1561 tatttagcgc ctgagtgagg cccggcgctt tggtatccgg cgcaacaacc tggctggcgt
     1621 gggcgtgatc agcggtgact atgaccagcg tgttaccctc ctttttagcg aattccagcg
     1681 cccgttgtac ggcttcatcg agatcgaccg tctcgccaat ttgcccacaa ggattcgcag
     1741 cgtgatcctg tttatcgatt gacgcacctt caacttgcag gaaaaatcct ttctcatttt
     1801 tactcaacaa ttcaatggtt ttgtcggtca tctgtgccag ggttggtaca ctgtcattac
     1861 gttgcggatt aggcgtacag gtgactgcgg gcttatcgat attgccgtgg tacgttgctt
     1921 tcggccctag ccagcgcact ggcatattgc cgtcagcaaa caggccaagc aggggttttt
     1981 gctgattcgc ttccgtcacc gaattcagtg aggcagcatc gttcaccaac tgataaccac
     2041 gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc accagcggtt gccgtttcag
     2101 caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg agcgttaagc agctgttcgg
     2161 taatcgatcc ttttccgcct ttttccagag cgttacccgg acatttttca ctggtcgcgc
     2221 tcggaccgta gcatttgcgc gaagtcacat gtgccaccag cgcagcgggc gtggcatcct
     2281 gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc ggcttttgcc atttccagaa
     2341 tcgttgggtg atctttttcg tgaatatcga cgcccagcgc gccgttatag gttttgacac
     2401 cggttgacca ggcggttgct gatgcagccg agtcggtgac gtagtccggt ttgccagttt
     2461 ttttattcag cgcatagtga gtgtattgcc cggtaagcgg taaggcatcg atacctttaa
     2521 aaaagccgcc cgcaccttcg gcataattac gtgcggcagt aatttccgaa tcccccatcc
     2581 catcgccaat cagcaaaata atattttttg caggtttatc gctaagagaa tcacgcagag
     2641 cggcggtctg atcactcgtt aagcggcgag caccgccggg tgcagtaata tcgccctgag
     2701 cagcccggtt ttccagaaca ggcatttctg gtgtccgggc ttttgtcaca ggggtaaaca
     2761 gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac tttattttct ccatgtacaa
     2821 atacattaaa aaataaaaac aaagcgacta taagtctcgg ccgtgacaac tttatgacag
     2881 ctgttgaaaa gattaacttt ttactgacga ggatgcttca ataacttctt tacgtaatcg
     2941 cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg gctttacctc gtacagcgcc
     3001 ccctctacct gatcaatcaa ccgctgttgg tcattttgcg ccatattgcg aagcattgca
     3061 gtgacgataa tctccaaagc ttctacctgc gcacacagtt ctttcgactc ttcttctttt
     3121 tgggcaagct taaataacaa ctcagcaatg agatttttca tgtctgtatt tccttatcca
     3181 aagtatggag aagttaacat tatgtttcac aacttcatag tgtctggaag tattattttt
     3241 atgacaaagg aaatactttg cgcaaatatt ttttgtttga ttaccagcga aacgtttctc
     3301 ttatttaaat aattacccac acaaaatat
//
LOCUS       DAZOYS010000294         3326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOYS010000294 DAZOYS010000000
VERSION     DAZOYS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.296"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..324)
                     /gene="fliZ"
                     /locus_tag="ACHUN9_004089"
     CDS             complement(<1..324)
                     /gene="fliZ"
                     /locus_tag="ACHUN9_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR0697341.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVR"
     gene            complement(370..1089)
                     /gene="fliA"
                     /locus_tag="ACHUN9_004090"
     CDS             complement(370..1089)
                     /gene="fliA"
                     /locus_tag="ACHUN9_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR0697342.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1254..2828)
                     /locus_tag="ACHUN9_004091"
     CDS             complement(1254..2828)
                     /locus_tag="ACHUN9_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR0697343.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3083..3326
                     /locus_tag="ACHUN9_004092"
                     /pseudo
     CDS             3083..3326
                     /locus_tag="ACHUN9_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          853 a          791 c          714 g          968 t
ORIGIN      
        1 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
       61 atcgaaaaaa tcactctgcg ttttgcagtg taaatcacca caaaatcggc acaatgccgc
      121 ccatgatttt tgttccactt gccagccatt ttcatcaagc agcatgtcca ggcgggaaat
      181 ctcgatttta ttcactattt tgccgtcgcg aacgagggta atgcggtcga gtaatttacg
      241 gcaatgcgcg caatgagtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
      301 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaaattaa agtgcggcat
      361 ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa
      421 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
      481 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc
      541 catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
      601 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
      661 gagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
      721 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
      781 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
      841 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
      901 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
      961 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
     1021 cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag
     1081 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
     1141 aggtttttta atcggacgat tagtgggtga aatgaggggt tatttagggg ttaccggtaa
     1201 attgcggaca gaaaaaaccc cgccggtggc ggggtttgag cgataagtgt aaattaacgc
     1261 agcagagaca gtacgttctg cgtggtctgg ttagcctgtg ccagaacaga ggtacccgct
     1321 tgttgcagga tctgcgcacg agacatgtta gacacttcgg ttgcgtagtc agcatcttct
     1381 atacggctac gggcagaaga caggtcgttt acggtgttgc caaggttggt gatggcagag
     1441 tcgaaacggt tttgtactgc acccaaagaa ctgcgcaact tatcaacttt tgccaaagct
     1501 tcgtctaatt tggataatgg atttgtggtt gctaaggctt tcaaatctga ggcactgagt
     1561 tcagcacctt tggttgccgt cttattcgtg atattggctg tataaacctt atcatcttca
     1621 cgcataattg cctgtgcaga accatcattt tttactgccc attcaactgc cttaccatta
     1681 ggatcagtaa taacagacgt gttacctgta gcggcatcta catttactgc aatgtaggtg
     1741 ccgtctgatg ttttagcagc aaatgaatta ggtacaggat tcccttgggt atcttgtact
     1801 tggtggacat ctaggttttt tcccccaaaa taaacagaca ttggtgcatc attataagct
     1861 aatgctttac ccattttacc cgcagctaaa tcaactgatg tggcagaacc attatcttca
     1921 acaaatgcag ttacacgacc accaaagttt gctaactgaa catccttacc acccacctga
     1981 actttttcat aacctgtaga gttttgtttc aataatgtaa aaccgttcgt gttggtatta
     2041 ttatcttgac caacaaaata agtctgccct tccacctgta cttgataaac ttttgttcct
     2101 gcagtaccgg tcgcatcagt tgtatccttt gtatcttgta cattaccggt accaaaataa
     2161 gtttcaacat cagcattttt ataattgcta ccgaatgctt tagcacctcc cacactacca
     2221 tcagttgcat ttaccttagt taagtcagga gcggcttcgc tcttaagtgc aaccgcagag
     2281 gacattggaa cttttcctag tgctacgcta aagttatcaa gccccaaggt tttagcatca
     2341 attttatcaa gggcaatctc aatggtttca ccatcgttcg cacctacttg aattttcata
     2401 gtctgatcag aagccagaac ttttacacca ttaaattgtg tctggttaga aacacgatca
     2461 atttcactaa ggcgttgtgt aatttcatcc tgaattgaat tcaggtcgga gtcagagttt
     2521 gtaccattag tggcctgtac tgacaattca cgaatacgct gtaagttatt gttgatttca
     2581 gaaagcgcac cttcagtggt ctgcgcaaca gaaataccat cattcgcgtt acgggaagcc
     2641 tgagtcagac ctttaatatt tgctgtaaaa cggttagcaa tcgcctgacc tgctgcgtca
     2701 tctttagcac tgttaatacg caggccagaa gagagacgtt caatggcgga gctcagagaa
     2761 gactgagatt tgttcaggtt attctgggtc aacagcgaca ggctgtttgt attaatgact
     2821 tgtgccatga tcttttcctt atcaattaca acttgatgtt attgggctgt tgcccacggt
     2881 ttctcaccgt accccttgta tcggcacctg aatttcgaac tttagaaaat tttagtttct
     2941 ctcctgtttt ttcttaacgg ctgaaatatc cgtaatatac aactctactc tgcgcatcat
     3001 cttcgtgaat caactattaa actttgcgca caaattgccg ataacccgct tatctactgt
     3061 tttcaatcaa aaggaattag gtatggcttc atttacatct cttggcgttg gttcgaatct
     3121 gccgctggat acattgctaa acaacctgac catcgctgaa aaaaaacgct taaacccaat
     3181 tactcagcaa cagagtgaca ataccgcacg tctgacagcc tatggcacat taaaaatgtg
     3241 cattagaaaa attccagact gcaaatactg cgttaaataa agccgatctg ttcaaaagca
     3301 ctaccgttac cagtagcaat gaagac
//
LOCUS       DAZOYS010000295         3216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOYS010000295 DAZOYS010000000
VERSION     DAZOYS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.297"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHUN9_004093"
     CDS             <1..495
                     /locus_tag="ACHUN9_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0697344.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHUN9_004094"
     CDS             525..3041
                     /locus_tag="ACHUN9_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697345.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="ACHUN9_004095"
     CDS             3052..>3216
                     /locus_tag="ACHUN9_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697346.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          939 a          771 c          730 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac gggacaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAZOYS010000296         3204 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOYS010000296 DAZOYS010000000
VERSION     DAZOYS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.298"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHUN9_004096"
     CDS             <1..369
                     /locus_tag="ACHUN9_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697347.1"
                     /translation="ELNDFLSMNESDLEVSSKKTSKQDREITDVSVDSDHKNTQCPAT
                     DDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSPKKKQT
                     QDDKKQKIKPKMYFAGGGIK"
     gene            369..659
                     /locus_tag="ACHUN9_004097"
     CDS             369..659
                     /locus_tag="ACHUN9_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697348.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            980..1270
                     /locus_tag="ACHUN9_004098"
     CDS             980..1270
                     /locus_tag="ACHUN9_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697349.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1270..1689
                     /locus_tag="ACHUN9_004099"
     CDS             1270..1689
                     /locus_tag="ACHUN9_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697350.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            1857..2180
                     /locus_tag="ACHUN9_004100"
     CDS             1857..2180
                     /locus_tag="ACHUN9_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697351.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2610..>3204
                     /locus_tag="ACHUN9_004101"
     CDS             2610..>3204
                     /locus_tag="ACHUN9_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697352.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1063 a          576 c          672 g          893 t
ORIGIN      
        1 gaattaaatg attttctctc tatgaatgaa tctgaccttg aagtaagctc taaaaagact
       61 tctaaacaag acagagaaat cactgatgtg agtgttgata gtgaccataa aaacacacag
      121 tgccccgcca ctgatgatga gttggacccc ttcgatgatg aagaggatga acgtgaaaaa
      181 gccattaaga gggcagagaa gaaaaagaga ttcgagcttg caagacaaaa gaacaaagta
      241 tcgggtttag acccattcga tgacagtgaa gatgattatt atctttcacc gaaaaagaaa
      301 caaactcagg atgacaagaa gcaaaaaatt aaaccgaaga tgtattttgc cggtggagga
      361 attaagtaat gaaagaatat cacggtgaaa agagatacaa agattacctg ttaaagcgtt
      421 acagcatttc tcgtgaaggt catcttatga aggatactca cggggaagtt taccggatta
      481 gaccaaagaa agaagggagg ggttatttct tttacgatgg tgtgacagat ttaaagattg
      541 acgctttgag gtttgcagtg ctgtatcatt ttgatatctg gaattcaatc caccagctac
      601 gattaaaaga cggtgacccc agcaacttaa aggccaccaa tattatcaca aaacgttagt
      661 ttgttttacc atcatcaatc gtaattgtga tttcatattt gaaaagctta atgctgaaat
      721 tgaatcgtgt acttctggcg ataatcattt ttaaaagctg atacacaaga agcaccagaa
      781 acacaaagca acctaagctt gcgaatctgt gagattcagc aattgtttca agaaagccca
      841 ttaattcccc ttatttatca aagggaatga cctgtatcag ccccctacaa ccatattaac
      901 atgtaaatga gttgttgaca atatgttata aatatgttaa agttagttat aagtcaaaga
      961 ttttaagaag gaaaatttaa tgagacacac agttacacgc cttttgccag aggcatattc
     1021 cagactggca gcaatcaaag acaaataccc agccatgagc cacacagcac ttgttggtga
     1081 gattctgaag gaacacacag cccttacacc gtttgacgct atcaagggcg ttgcagaggg
     1141 ttctaaggcc atcagaatca caacgggcca actggaacta ctcaaagcac agtccgcagt
     1201 ggcaggttgc tcaatgattc aggtgttgag tgccaaggtt catcaactgg cggataagct
     1261 gggggtttaa tgctatgcac atttgattat catgcgtttt atcgccatag aggtcacctt
     1321 gtccgtgaga ataacggaac atggaaagat ttaactcgta atgctgaacc cttcacacac
     1381 cagcaattaa aatcccgctt ctccagatgg aagaatgcac cagcagatac tggtgatttt
     1441 gagattatcc ataccttcga ttatgcctca gaagatgacc ttcacgatgc aattaagctg
     1501 attgaagact tcaatacctc agaagatgtg gatggtgaca aatataaatc ccgtggtaac
     1561 cgctataacc gtgatgatat cactcaggcc ataaatgctg gtcaatctca gcgatacatt
     1621 acagaaaaat atggtatttc acggagacag tatttctacg ttaagtcatt gattaataaa
     1681 gacaattaaa aataaccatc taagaaacgg tagaatgcac tttacaggca gagttgcacc
     1741 ttaatttgat aaattacctt accagccagt gcaactccct ctgtaacccg catggttact
     1801 ggcctcagaa tggctatttt aagaggtctg aggtgtgttt cataggagat ttaaaaatga
     1861 ataattaccc aatgcaaatt atggttgata acgacactgc tatgatggtg caaagcttca
     1921 ttgatgcagg tatcgcaatt aattttgata aattactaaa gctgatggct gataatgcag
     1981 aaaacatctc tgactttatt cagtcggtgg agtttaacga gcctcgaatg atgctaccaa
     2041 ttacagacag caacatgaaa agattggtta tcgagcaaac gaacatatac agtatttccc
     2101 cagagcaatt tttgaaaggt gctgtgacaa tcctgtacag tgataatatt ctggtgacgg
     2161 attctgtgag ggttcactaa tgggtttaat aattgcttgg acgttgcttg gcttatttgt
     2221 tggttgcatt attaacaatg cggtaaataa gaattgatgt tatcaacctg ttaaaaagct
     2281 gttgacaaat aaccttaagc atggcataat tttaattgtg ggagagcaat tctctcttat
     2341 ttttctttca ataatccgat ggatggatat tctgagttgg aaaggtgcaa tgcttttcgt
     2401 catggccttt caggttagtc tgagaggcta tctcaaaaag tactcttgtt cattcattat
     2461 tgaatccttt gcccttcaca gaaatgttta gggctttttc agtttttaaa agcacacttg
     2521 gcaggttctt gttcagtcag actcttcacc tctgattgct gagtgtgctt ctataaattg
     2581 aaataacaaa taaactaagg ggtgaacgaa tgattagaaa tgaacatgaa atgcttatcg
     2641 aacacagacc aagtgcactt gatattcacg aattaacttt gcgtgaatct tttgatagag
     2701 cttggcggga atcacacgaa gtaaataaag gtccatatat tccaccagaa ccactcgaga
     2761 ttccaagagt tgaaattgat ttttctatga acgaacggtg tgaacttgat ttgaagttga
     2821 agctacaacg ccgatatttc caagctatta aagactctca agaagcaatg agaagtgttt
     2881 acgagaaaat tcagtccaag cttgaagacg aaattgacga aatggttatt tcgatggagc
     2941 ttcaaaccaa ttcttatgct tgtttctctc gactagaccg tgacggttgg gggtatgacc
     3001 ctgtagagat tggtaaagtg attgcagatt taccagaagg tcaaagagtt gttcgtacag
     3061 aaagagctgt cagagcacca catcgactgt ctctaattgt tgctgataaa tcacaagctg
     3121 agcttgaaga aattgccaaa gagaaactga tgaaagcaag agaaaatgag attaatttgc
     3181 tgaaatcaag actggctaag ctga
//
LOCUS       DAZOYS010000297         3200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOYS010000297 DAZOYS010000000
VERSION     DAZOYS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.299"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            83..799
                     /gene="nanC"
                     /locus_tag="ACHUN9_004102"
     CDS             83..799
                     /gene="nanC"
                     /locus_tag="ACHUN9_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0697353.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            819..1925
                     /gene="nanM"
                     /locus_tag="ACHUN9_004103"
     CDS             819..1925
                     /gene="nanM"
                     /locus_tag="ACHUN9_004103"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0697354.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1990..2970
                     /locus_tag="ACHUN9_004104"
     CDS             1990..2970
                     /locus_tag="ACHUN9_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0697355.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3080..3200
                     /locus_tag="ACHUN9_004105"
                     /pseudo
     CDS             3080..3200
                     /locus_tag="ACHUN9_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          942 a          678 c          755 g          825 t
ORIGIN      
        1 caaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
       61 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
      121 gtgcttttcg tcaccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
      181 tagcggaagc catgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
      241 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
      301 cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
      361 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
      421 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
      481 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
      541 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
      601 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
      661 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
      721 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
      781 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
      841 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
      901 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
      961 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     1021 ctgcattccc tggtggaccg agagatcaag caacctctgc atttattgat ggcaatctat
     1081 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     1141 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgctcgcg ccgatgggca
     1201 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     1261 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     1321 tagataaaat caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt
     1381 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     1441 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     1501 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     1561 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     1621 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     1681 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     1741 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     1801 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     1861 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     1921 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     1981 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     2041 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     2101 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     2161 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     2221 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     2281 catattgcac ggaaattact gcccttcatt cctaataatg cagggattct tatcgttccg
     2341 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     2401 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     2461 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     2521 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     2581 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     2641 actgacgcgc catggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     2701 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     2761 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     2821 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     2881 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     2941 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     3001 gttcgttctc agagtagaca acaaaaaaca aataagatca aggtaatatc tcttcacttg
     3061 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     3121 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     3181 cttttcgatt atggctcgga
//
LOCUS       DAZOYS010000298         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOYS010000298 DAZOYS010000000
VERSION     DAZOYS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.300"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUN9_004106"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUN9_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0697356.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUN9_004107"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUN9_004107"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0697357.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUN9_004108"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUN9_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0697358.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUN9_004109"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUN9_004109"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0697359.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUN9_004110"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUN9_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0697360.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZOYS010000299         3081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOYS010000299 DAZOYS010000000
VERSION     DAZOYS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.301"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..2839)
                     /locus_tag="ACHUN9_004111"
     CDS             complement(<1..2839)
                     /locus_tag="ACHUN9_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0697361.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKK"
     gene            complement(2896..>3081)
                     /locus_tag="ACHUN9_004112"
     CDS             complement(2896..>3081)
                     /locus_tag="ACHUN9_004112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697362.1"
                     /translation="TSEQHRCSNEKKEFQRRVYLRGDAFINLYTNFYSGIMTGLQLLR
                     HFQRIALPAYHPTFFIP"
BASE COUNT          774 a          818 c          746 g          743 t
ORIGIN      
        1 ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga taagtctgca
       61 aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac acatcaccct
      121 gccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc agaaaaagct
      181 tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg ctgatataac
      241 tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca cactctgcca
      301 gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc gtgtggaaca
      361 gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt ttgatgaggc
      421 gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac tgcgcacttt
      481 tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga taagccgcca
      541 gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac tgaaccagtg
      601 caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc gaacggtgat
      661 caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt gagtatggcc
      721 ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt gtgggcttaa
      781 aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga ctgtactcca
      841 gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt ttccactgtc
      901 gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg atccggctac
      961 ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg gttttctgcc
     1021 actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga tgcgtgagcg
     1081 caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca ttgtgtgtag
     1141 cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc gctgaacgat
     1201 cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc ggtgtaagac
     1261 cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa aggatcaccg
     1321 tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta ctgaaccagc
     1381 atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga ttcgaaagtg
     1441 aggttttggc attcagttcc tgctttgtct cttccctgtc gtccagcaac cggatggtcc
     1501 cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata gcactaaagc
     1561 ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc ccgttacggt
     1621 aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg aaaaagagac
     1681 ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca tcaccacaga
     1741 gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga acaggcgtca
     1801 tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac gaaagatact
     1861 gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg atataacgtt
     1921 caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc cgtatcccca
     1981 gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca ccgatcacgc
     2041 ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc ctgttgcggg
     2101 gcagccagtc gctgaacgca ctgaaagtca cctccctgcc ttccggaatg cgcagagttt
     2161 ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct tcccctgaag
     2221 cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga tacagtatat
     2281 caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt cccgccgagc
     2341 caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca tcaaaggaca
     2401 gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc agcgcttcag
     2461 tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca tcaatcacca
     2521 cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat tccagaacaa
     2581 ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc cggatactga
     2641 gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca aagttatcgg
     2701 tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc agagtatgat
     2761 aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg attttacgaa
     2821 ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg gaacaatcag
     2881 aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga gggcgatccg
     2941 ctggaaatgg cgcagtagct gcaggcctgt catgatgccg gaatagaagt tcgtgtacag
     3001 gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt tttcattgga
     3061 gcacctgtgt tgttctgagg t
//
LOCUS       DAZOYS010000300         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOYS010000300 DAZOYS010000000
VERSION     DAZOYS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.302"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUN9_004113"
     CDS             <1..173
                     /locus_tag="ACHUN9_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697363.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="ACHUN9_004114"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="ACHUN9_004114"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR0697364.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="ACHUN9_004115"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="ACHUN9_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="ACHUN9_004116"
     CDS             2191..>3046
                     /locus_tag="ACHUN9_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0697365.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          804 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcactg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAZOYS010000301         3025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOYS010000301 DAZOYS010000000
VERSION     DAZOYS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.303"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            143..856
                     /locus_tag="ACHUN9_004117"
     CDS             143..856
                     /locus_tag="ACHUN9_004117"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0697366.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            888..1646
                     /locus_tag="ACHUN9_004118"
     CDS             888..1646
                     /locus_tag="ACHUN9_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697367.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1692..>3025
                     /locus_tag="ACHUN9_004119"
     CDS             1692..>3025
                     /locus_tag="ACHUN9_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0697368.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          681 a          734 c          813 g          797 t
ORIGIN      
        1 aaatatttat ttggttaata tgtttttatg aaagcgtaat tcaggtcaat gtcacaatta
       61 accattgtca caataaggtt gaacggatat tctgagcacg gaccacgtaa tattcaatac
      121 attatttact gccgggtttt tcgtgaatca acgtaatatg tcaataatta attccacgcc
      181 agtgcgtgtt attgccattg taggatgtga tggttcaggt aaatcgaccc tcacggcaag
      241 cctggtaaat gaactggcag caagaatgcc aacagaacac atttatctcg ggcaatcgtc
      301 cgggctaatt ggcgaatgga tttcacagct ccctgttatt ggcgcacctt ttgggcgtta
      361 tctgcgaagt aaagcggcac atgtgcacga aaaaccctca acaccgcctg gcaatattac
      421 tgctctggtt atctatctgc tttcctgctg gcgggcgtac aagtttcgca aaatgttgtg
      481 taaaagccag caaggctttc tgctcatcac cgaccgctac ccgcaggttg aagtgccggg
      541 ttttcgcttt gatggcccgc aattggcaaa aaccacgggc ggtaacggtt ggataaaaat
      601 gttaaggcag cgcgagctga agctgtacca atggatggca tcttatttgc ccgtattgtt
      661 gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt aaacctgacc accaactggc
      721 agcgttacag gaaaaaatcg ccgttacgcc gcaactgact ttcaacggcg ccaggatcct
      781 tgaactggat ggtcggcaac cagccgatga aatcctgcaa gcgtcactac gtgcaattca
      841 cgccgcgctt tcctgatcac caaaatggat aggggatgat ttaacgaatg gatgcactac
      901 aaacacaaac tgttaatagt acaaccgcgc cgcagcccag ctacattccg gggctaattg
      961 cggtggtcgg gtgcgatggc accggtaaat ccacgctgac caccgatctg gtgaaatcat
     1021 tgcaacagca ctggcaaacc gagcggcgct atctggggct gctctcgggc gaagacggcg
     1081 acaaaatcaa acgattaccg ttggttggcg tctggctgga acggcgactg gcggccaaat
     1141 cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct atgggcggcg gtgattatgt
     1201 actgcttctc gctgcgaaga atggcgaatc tacgcaaggt tcagcgactg gcgcaaagtg
     1261 gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat ttcgggcttt tattacgacg
     1321 gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag catgtttctg gcgcagcgcg
     1381 aacggcgttt ataccaacaa atggcgcaat atcgcccgga attaattatt cgcctgggca
     1441 ttgatattga gactgccatc tcccgcaagc ctgaccatga ctatgccgag ctgcaggaca
     1501 aaatcggtgt catgtcgaag attggctata acggcacaaa aattcttgag attgattcca
     1561 gagcgcccta cagcgaagtg ctggaacagg cacagaaggc ggtttctctg gttgccatcg
     1621 tttctgaccg ccgaagttta acttaaggct ggcgggatac tttttactat caacgataag
     1681 gtcaggctga attggcgggt tttaacatca aacattggtt tgcagatggc gcgtttcgca
     1741 ccattattcg caatagcgcc tggttaggct ccagtaatgt cgtgagcgcc ttgctggggc
     1801 tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc catgtttggc gtactggtga
     1861 ttgtgcaatc gtacgccaag tcgatcagcg attttattaa gtttcagaca tggcagctgg
     1921 tggttcagta cggaacgcca gccttgacca acaataatcc gcagcaattt cgcaatgtcg
     1981 tctcattttc cttctcgctg gatatcgtca gcggcgcggt ggcgattgtc ggcggcattg
     2041 ccttactgcc attcctttcc cattcattag gtctggatga ccaaagcttt tggctggcgg
     2101 cgctgtattg cacgctcatt ccttcaatgg cttcctccac gccgaccggc attctgcgtg
     2161 cggtagatcg cttcgattta attgctgtac agcaggcgac gaaacctttt ctgcgcgcag
     2221 cggggagcgt cgtagcctgg tattttgact ttggttttgc gggttttgtt attgcctggt
     2281 acgtgtcgaa tctggttggc ggcaccatgt actggtggtt tgcagcgcgc gaattacgcc
     2341 gcagaaatat ccataatgcc ttcaaattga atctgtttga gtctgcccga cacatcaaag
     2401 gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc catctggtcg gcgcgtaact
     2461 cgtgcagcac agtgctggtg gggatcgtgt taggacccgc tgccgccggg ttatttaaaa
     2521 tcgccatgac attcttcgac gccgccggaa cgccagcggg tttgctgggt aaaagttttt
     2581 acccagaggt gatgcgttta gatccgcgca ccaccagacc gtggttgtta ggtgtgaagt
     2641 ccggtttact ggcgggcgga atcggtattc tggtggcgct cgcggtgttg atcgccggca
     2701 agccgctcat ttcgctggtg tttggcgtta agtatctcga agcgtatgac ctgattcagg
     2761 tgatgctggg cgcaattgtg atttcgatgc tgggtttccc gcaggagtca ttgctattga
     2821 tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat cgcctcaatc ggctacatcg
     2881 tattgctgtt catgttctgt catctgtttg gtgtgctggg agcagcgttt gcttacttcg
     2941 gtgggcaatg tctggatgtg gtgctctcgt taattccaac tttaaaggca ttttttcagc
     3001 gtcattcctt gctttataac gcagc
//
LOCUS       DAZOYS010000302         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOYS010000302 DAZOYS010000000
VERSION     DAZOYS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.304"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUN9_004120"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUN9_004120"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0697369.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUN9_004121"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUN9_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0697370.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUN9_004122"
     CDS             2805..>2992
                     /locus_tag="ACHUN9_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0697371.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZOYS010000303         2980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOYS010000303 DAZOYS010000000
VERSION     DAZOYS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.305"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(126..202)
                     /locus_tag="ACHUN9_004123"
     tRNA            complement(126..202)
                     /locus_tag="ACHUN9_004123"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Asp,seq:gtc)
     gene            complement(335..1066)
                     /gene="dnaQ"
                     /locus_tag="ACHUN9_004124"
     CDS             complement(335..1066)
                     /gene="dnaQ"
                     /locus_tag="ACHUN9_004124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0697372.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1131..1598
                     /gene="rnhA"
                     /locus_tag="ACHUN9_004125"
     CDS             1131..1598
                     /gene="rnhA"
                     /locus_tag="ACHUN9_004125"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0697373.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1595..2317)
                     /locus_tag="ACHUN9_004126"
     CDS             complement(1595..2317)
                     /locus_tag="ACHUN9_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0697374.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2351..>2980
                     /gene="gloB"
                     /locus_tag="ACHUN9_004127"
     CDS             2351..>2980
                     /gene="gloB"
                     /locus_tag="ACHUN9_004127"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0697375.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNE"
BASE COUNT          810 a          724 c          695 g          751 t
ORIGIN      
        1 atatgtgatt aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt
       61 aaatgcaaaa aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt
      121 gaatttggcg gaacggacgg gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc
      181 taaccgactg aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga
      241 ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc
      301 ccaagccgct atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc
      361 ccttcttctc caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa
      421 aaacaacgcg taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt
      481 gctgctgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca
      541 gataaacttc cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt
      601 tatctatttc gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt
      661 tcctcgccac cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc
      721 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
      781 ccaactccgc gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct
      841 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
      901 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
      961 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg
     1021 tggtttcggt atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
     1081 tcagacttat cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac
     1141 aggtagaaat tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg
     1201 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca
     1261 acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg
     1321 aagtcatttt gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata
     1381 actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct
     1441 ggcaacgtct tgatgctgcc ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc
     1501 atgccggaca cccggaaaac gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc
     1561 ccacactgga agatacgggc taccaagtcg aagtttaagc ttgtggttta cgacattgcc
     1621 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta
     1681 gcgttaaagg aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa
     1741 tatgcgcatt caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg
     1801 cgtgcagcac ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca
     1861 tccggctgtt atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta
     1921 aactaatggg attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg
     1981 tttcacgcaa taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa
     2041 catcaacgga tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca
     2101 ttccttgcgc agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg
     2161 cgcttaaatt gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga
     2221 gctggcgctc cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag
     2281 gagccacgac agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactaa
     2341 gaggtaatct atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt
     2401 gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa
     2461 cgccattgcc gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga
     2521 tcacgttggc ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc
     2581 acaagagaca caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt
     2641 tttggggcat gaatttagtg taattgccac accgggtcac actttaggac atatctgtta
     2701 cttcagtaaa ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtaggtt
     2761 gtttgaaggg acagcatcac aaatgtatca atcacttaat aagttaagtg cgttacctga
     2821 cgatacattg gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag
     2881 tattcttccg cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc
     2941 aaaaaatcaa ataacactac ccgtaattct gaaaaatgag
//
LOCUS       DAZOYS010000304         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOYS010000304 DAZOYS010000000
VERSION     DAZOYS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.306"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUN9_004128"
     CDS             37..264
                     /locus_tag="ACHUN9_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0697376.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUN9_004129"
     CDS             complement(265..639)
                     /locus_tag="ACHUN9_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0697377.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUN9_004130"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUN9_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUN9_004131"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUN9_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0697378.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLINHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          694 c          689 g          794 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgatcaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZOYS010000305         2938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOYS010000305 DAZOYS010000000
VERSION     DAZOYS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.307"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHUN9_004132"
     CDS             complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHUN9_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0697379.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1761..1836
                     /locus_tag="ACHUN9_004133"
     tRNA            1761..1836
                     /locus_tag="ACHUN9_004133"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Asn,seq:gtt)
     gene            complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHUN9_004134"
     CDS             complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHUN9_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0697380.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          863 a          784 c          620 g          671 t
ORIGIN      
        1 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
       61 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
      121 acacaactgg ctttttttca cacttttgcg gttcggatcg agggtatttc cacagccaac
      181 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
      241 ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa
      301 cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca tcgcgagcac
      361 ctttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta
      421 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
      481 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggaaagcc
      541 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
      601 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
      661 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
      721 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgcggga ataccaatcc
      781 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
      841 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
      901 gagaaatggt taaacccagc cctgccccga caaatcccag tcccggccag gagaaaaggc
      961 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcaata
     1021 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaataaga gtgatggcag
     1081 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtgct ttaacttctg
     1141 tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga ataagcgttg
     1201 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     1261 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     1321 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     1381 catcttttcc cagccagctg accagaaaag tgctcagaac ccccatcaac aggacacagg
     1441 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     1501 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     1561 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     1621 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     1681 gaaacggcaa acagttgggg aatttaaaaa tcgggtttga caaaagattt ttcgccgtta
     1741 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     1801 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat
     1861 agcgggcttt ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctgcc
     1921 gcttgatatc ctcaactgac agtcctgcat tacaaagttc cagaaagcgc cagacatagt
     1981 tacgctgaag ttgtcctcgc ttcagtccca accagacagt attagcatcg aaaagatgcc
     2041 gcgtatccag acggattaaa ttctcttcct cttgttcgcc gctggattgt tcggcaacta
     2101 atccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta
     2161 atacaatatc tgccagcaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg
     2221 taatcccctg tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg
     2281 taatttgcgt taagggatga tcgagtggaa caagcaaact atggtgccaa cgaaaccacg
     2341 ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt
     2401 cgccattttg caacaacgtc gcaatttcct gtggcgtccc ctggattagc tcgagccgaa
     2461 cctccgggaa aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag
     2521 tatgcgtcgt tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc
     2581 gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg
     2641 gttcagtcat gcccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat
     2701 cctccagttc ccgaatatga cggctgacgc cggactgtga ggtaaaaagc atattcgcaa
     2761 cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat
     2821 tcacggtaaa ctccgggcag ttcagatttc ccgttattgt taaagtctaa tgcccggcat
     2881 aacaaataat aaaaacccgc atcttattcc atcccgatat aacgcttagc tcaccaat
//
LOCUS       DAZOYS010000306         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOYS010000306 DAZOYS010000000
VERSION     DAZOYS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.308"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUN9_004135"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUN9_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0697381.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUN9_004136"
     tRNA            1492..1586
                     /locus_tag="ACHUN9_004136"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHUN9_004137"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHUN9_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZOYS010000307         2880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOYS010000307 DAZOYS010000000
VERSION     DAZOYS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.309"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUN9_004138"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUN9_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0697382.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUN9_004139"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUN9_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0697383.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHUN9_004140"
     CDS             1650..2099
                     /locus_tag="ACHUN9_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0697384.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUN9_004141"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUN9_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0697385.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUN9_004142"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUN9_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0697386.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2880
                     /gene="yncL"
                     /locus_tag="ACHUN9_004143"
     CDS             2787..>2880
                     /gene="yncL"
                     /locus_tag="ACHUN9_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0697387.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          759 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttattt
//
LOCUS       DAZOYS010000308         2841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOYS010000308 DAZOYS010000000
VERSION     DAZOYS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.310"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            69..1010
                     /locus_tag="ACHUN9_004144"
                     /pseudo
     CDS             69..1010
                     /locus_tag="ACHUN9_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1010..1108
                     /locus_tag="ACHUN9_004145"
                     /pseudo
     CDS             1010..1108
                     /locus_tag="ACHUN9_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUN9_004146"
                     /pseudo
     CDS             1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUN9_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2613..2714
                     /locus_tag="ACHUN9_004147"
                     /pseudo
     CDS             2613..2714
                     /locus_tag="ACHUN9_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          773 a          559 c          749 g          760 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaact tttttaagat
     1021 tttacagtcg cttgaactct caatgacgct atgcgacgcg aaaaatgcct cgccagaagc
     1081 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     1141 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     1201 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     1261 atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     1321 cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga tttgttgtca
     1381 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg
     1441 cgtccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt attaacggct
     1501 tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt
     1561 gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa
     1621 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag gcagcccaat
     1681 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     1741 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt gcgcgcgtta
     1801 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     1861 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     1921 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gctgaaagat
     1981 cgctatgatt ttatgaagtt tcaagtgttc cagtggttga ttggcgcaac ggatggccat
     2041 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     2101 gacatcattt cagcatttcc ggtccttggc ggtacgggaa taacatcagc gatctcaaac
     2161 tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc
     2221 gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc
     2281 taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga
     2341 cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac
     2401 ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcaa
     2461 tcatgaccgg ccaacgactg gattctccct tggtttatgt ttgtgcgcat aatgtatatt
     2521 atgttaaatc atgtctgagt gatgcgaagt gcttttctgg tagtcgttat tcgttcagat
     2581 taggtgtcct tgtgatttaa agatgttatc gtggcacgaa tatactgcca ttccaggctc
     2641 gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg accttcaaag
     2701 ttcaatatct ataattcaat cttaaggtgg ctatacgtca tctgaaattg aaacagggtg
     2761 tgtgtggcat ggctacgcac acccgagcaa ttcaccaatg tgaatctcat tattaatgag
     2821 gagtggtttg tatttggagc g
//
LOCUS       DAZOYS010000309         2792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOYS010000309 DAZOYS010000000
VERSION     DAZOYS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.311"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            147..1199
                     /locus_tag="ACHUN9_004148"
     CDS             147..1199
                     /locus_tag="ACHUN9_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR0697388.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1515..2717)
                     /locus_tag="ACHUN9_004149"
     CDS             complement(1515..2717)
                     /locus_tag="ACHUN9_004149"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0697389.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT          664 a          706 c          652 g          770 t
ORIGIN      
        1 cggtaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctatg catttgtctt
       61 taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac atttcattat
      121 tagcagggat acctgaggag tttaaagtga acatatatat cgggtggctt ttcaaattaa
      181 tccctttgat tatgggatta atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag
      241 ggcaaagcga gtatttcgtc gcgggtcatg tgctgatttc tctggcggcc atatgcctgg
      301 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
      361 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
      421 gggcactgtt agcaggcaat gatgtgatgg cagacgagtt tgtcgccggc catgttattt
      481 tcggcgttgg tatgattgcc gcctgtgtat cgacggtggc agcgtcatcc ggtcactttc
      541 tgctcattcc caaaaatgca gcggggagca agagcgatgg aacgccggta caggcttatt
      601 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
      661 tttggtctat tacgctgtta cgtagtgctg acataactcc gcattatgtc gcgggtcacg
      721 tattgcttgg gttaaccgca atctgtgcct gtctaattgg ccttgttgcc acaattgtcc
      781 atcaaacacg taatacgttt tcaactaaag aacactggct gtggtgttat tgggttattt
      841 ttctcggctc aatcacggta ctgcagggga tatacgtctt agtcagttcc gatgcaagcg
      901 cccgactggc tcccggcatt attcttattt gcctcggaat gatctgttac agcatattct
      961 caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc aacagaatac
     1021 cgatgatacc cgtcttcacc tgcctgtttt gccttttcct ggcatcattt cttgcggaaa
     1081 tggcgcagac cgacatggga tattttattc cttcgcgagt tctggtcggt ttgggagcgg
     1141 tatgctttac gttgttctca atcgtttcaa tattagaagc gggttctgct aaaaaataat
     1201 tgcaacgtac cggataaaac cagcgttgac catttgcgta acgctggttt ttcttaggca
     1261 tcatgaaata acgcagcatt aatgcatagt ggttaaggat aaaaaaacaa caaagttact
     1321 gtctttttca gcctgttcat attttataaa gatctggcca ctgggtgagt tttcaatcac
     1381 ctttccttcc accttatatt aagcctggag ggtttcagtt cacgggctca ttagaaaata
     1441 atcacaagat agccactatc aaaattagct catttttaat gcggctctaa taattttcat
     1501 ctttaggtaa caggtcagga cgacttcgct gcaatcaaat cctgcgcaca agcccacgca
     1561 ctcgaccacg cccactggaa gttatagccc cccagccagc cggtaacgtc catcacttcg
     1621 ccgatgaagt acagcccagg cactttgcgc gcttccatcg tccgtgaaga gagttcgttg
     1681 gtgtccacgc cgccgagcgt cacttcggca gtgcgatagc cttcagtgcc gttgggttgt
     1741 acgcgccagt cggtcaatgt gctaatcagt gcctgttggt cacgcacgtt gagctgtttt
     1801 agcgaaacat tcgggatttg cccgagttgc tgtaagcgtt caaccaaccg cttcggtaga
     1861 tgaaccgcca gtgtgttttt caggctctga ttcggatgtg cgttacgctg ctcattcagg
     1921 aaggtttcga gatccacatc cggtagcaga ttgatactga caaattcacc cggttgccag
     1981 tagctggaaa tctgcaacac cgccggtcca gacaagccgc gatgggtgaa gagtaagttc
     2041 tcacggaaaa cgatgccgtt ttcagcggta atcacggaag gcaccgccac gcccgccagc
     2101 acctgtaact cttcgagcaa cggtttatgc agagtgaatg gcaccagacc cgcacgggtc
     2161 ggcagcacgt tgaggccaaa ttgttcggca atcttataac caaacggcga cgcgcccagc
     2221 cccggcattg acagcccacc ggtcgccatg accagctttt cgcaaccgac agtcatgccg
     2281 ttcagatcaa gcgtgaagcc tgtttcatcc tccgccacac tcagcacttc gctacgcaat
     2341 ctgaaggtca cattgccctt ctcgcactca tccaccagca tgtcgacaat ctgctgcgcg
     2401 gagtcatcgc agaagagttg ccctaacgtt ttctcgtgcc atgcgatgcc gtgtttattg
     2461 accagatcaa tgaaatccca ctgggtaaaa cgtgcgagtg cagacttaca aaaatgcgga
     2521 ttctggctca gataagcgcc tggttcgaca taaaggttgg taaagttgca gcgcccaccg
     2581 ccagacataa ggattttgcg ccctggtttt ttaccattat cgatcagcag aacccggcgt
     2641 cctgcctgac ctgccagcgc agaacagaac atacccgccg caccagcgcc tataataatg
     2701 gcatcaaacc tttccacgtt gcgctcctct tagaaaaaac gggcgtgaat tgtaaagatt
     2761 cctcagtggt cgcaccagca tcaatattac ta
//
LOCUS       DAZOYS010000310         2762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOYS010000310 DAZOYS010000000
VERSION     DAZOYS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.312"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            74..2353
                     /gene="ydeP"
                     /locus_tag="ACHUN9_004150"
     CDS             74..2353
                     /gene="ydeP"
                     /locus_tag="ACHUN9_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0697390.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2601..>2762
                     /gene="safA"
                     /locus_tag="ACHUN9_004151"
     CDS             2601..>2762
                     /gene="safA"
                     /locus_tag="ACHUN9_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0697391.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARD"
BASE COUNT          734 a          645 c          676 g          707 t
ORIGIN      
        1 taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
       61 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgcag gtggttgggg
      121 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc atgatgttat
      181 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
      241 tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      301 agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact
      361 tacctgggga gatcacgagc ttgaggctgc agggcgtctc actcagcctt tgaaatatga
      421 tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
      481 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
      541 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca ataactttcc
      601 cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt
      661 aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
      721 gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg
      781 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc
      841 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca gtgcctacta
      901 caacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
      961 gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg aatttattca
     1021 aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga
     1081 tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg acgcatatgc
     1141 cgctgccgat cgcaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     1201 ccagaatgta cagcaactgg tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg
     1261 tgcgggtatc tgcccactac gaggtcactc taatgtacag ggcgaccgaa ccgtcggtat
     1321 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcacccc
     1381 acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgca cggggcaggc
     1441 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     1501 cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc ttaaccgctc
     1561 tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga
     1621 catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
     1681 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc
     1741 gggaatcgcg caggcagcac taccccacag cgtggtagcc tgggagtatc tggtggaaga
     1801 ttatgatcgc attcgcaatg acattgaagc tgtgttacca gagttcgccg actataacca
     1861 gcgcatccga catcctggag gttttcacct gataaatgca gctgctgaaa ggcgctggat
     1921 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     1981 agcgtttaac agtaagctgg tcatggcgac agtacgtagc cacgatcagt acaacacgac
     2041 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     2101 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     2161 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     2221 ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaactct
     2281 tgataaccac gatccattaa gtggcattcc tggttataaa agtattccgg ttgaattaga
     2341 accatcaaat taattcgcct cctcatttct tctgctgtca tccgcacagc agaagaattt
     2401 ctcattgact attatcgcgc aatttgctca cattgattaa attaaaccac acactataag
     2461 atataaacct ctgcctacag ctgtaagaaa ctccgctcag tactgaagtg ccagtcctat
     2521 ttcctctttt ctccagcctg ttatattaag catactgatt aacgattttt aacgttatcc
     2581 gctaaataaa catatttgaa atgtatgcga ccacagtgaa aaacaaaatc acgcaaagag
     2641 acaactataa agaaatcatg tctgtaattg tggttgtctt attactgacc cttacgttga
     2701 tagccatttt ttcggcaatt gatcagctgg gtatttcaga aatgggtcgc attgcaagag
     2761 at
//
LOCUS       DAZOYS010000311         2761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOYS010000311 DAZOYS010000000
VERSION     DAZOYS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.313"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1363)
                     /locus_tag="ACHUN9_004152"
     CDS             complement(<1..1363)
                     /locus_tag="ACHUN9_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0697392.1"
                     /translation="MNRTSPHYCRRSVLFLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1428..1547)
                     /locus_tag="ACHUN9_004153"
     CDS             complement(1428..1547)
                     /locus_tag="ACHUN9_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0697393.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1771..1929)
                     /locus_tag="ACHUN9_004154"
                     /pseudo
     CDS             complement(1771..1929)
                     /locus_tag="ACHUN9_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2274..>2761)
                     /locus_tag="ACHUN9_004155"
     CDS             complement(2274..>2761)
                     /locus_tag="ACHUN9_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697394.1"
                     /translation="FPEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLG
                     GLDFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGL
                     SGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSF
                     Q"
BASE COUNT          748 a          657 c          512 g          844 t
ORIGIN      
        1 caactaaagc actgcccgtc tcctggttaa ccccatgtgc tataccgccg gtatagacaa
       61 tcagcgagcc tccggcgcta atattgctgc cagttgccgt accatctttc tcaacaacct
      121 gccggctccc ggtggaaata tttgtcgtgt cagctttccc gccgctcttg atattttgtg
      181 ttccgctgtt gatattggta cccgtggcaa taccatgatt attaatattc tgtgtaccac
      241 cattgattat ggtattggtc gcgtttctgg taacatccat taccccgccg ttatctattc
      301 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatct ttaataatcg
      361 tcttatttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
      421 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
      481 taccgttagt gtttgttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
      541 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
      601 aatcaacaat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttattat
      661 tcagagactg agttccacct tcaatcgtcg tgtccagcgc atggctttca tatactcttt
      721 gctcaccgcc atttttaatg gtggttgttc ctactgtgct ctgttcaaca tactgtcgtc
      781 caccatttat gattgtgttc gttgccatac ttccttgcgt gatgtcctga taaccggact
      841 tatttatcgt tgtaccatca acatgcccct gagttgttac tgtctgccct ccaccatcaa
      901 tgatggttcc attcgcagtc cccccactta tgtgtgaatt accaccctta attgtcgttc
      961 cattgctgat accacctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg
     1021 aaatgccccc gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagactgcc
     1081 ccatataatt gttattcctt ccaacatctt gatatccacc agattcaata agacttccat
     1141 tagatactcc gccataaaca ctctgctggc catggttgat aatatgagtg ttatttgttg
     1201 tacctcgttc atctactcgt tggttgccat ctacagtctc atcgtttacc acaccaataa
     1261 cattagtagt aaaggccgcc atcacgggcg gggcatatat caaggcagat atcaataaga
     1321 aaagtactga gcggcgacaa taatggggac tggtcctgtt cataaatttc atcctctgaa
     1381 aagtgaatac tgagtagcgt ttaagcgact ttagctctgc tgcaacatca gcccgcaggc
     1441 accagaccgg ggggttcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     1501 aaagacatca tgatgaaatg atgatgttcc acataagaaa gaggcatttt ttaaacacag
     1561 tgcgctgaag tgtgattgga taaaaaagtc aatctattca ggaaatacgg gcgtattctt
     1621 ttctttcgac aatcaggccg tcggcaaaat aaaatgattt acataatcgt ttctgatgaa
     1681 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattc ttttactttt
     1741 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     1801 accacgtttg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     1861 gtgcggttta cagactaccc gtgcgtccac cgccgataat gtcctgagca gttttttcag
     1921 tccggacctc ctgccacggt tttacgttga agaggtaacc ctgagcacgc agttcttcag
     1981 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     2041 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattta
     2101 aactcagtaa atcacagcac agatcaaggg gttacgtgaa agcgtagccc ctttttcttt
     2161 ggtagtggca gcaaaatggt tgtagtgtaa aaaataatcc cgtttaatca atcaataata
     2221 catattgttt caatctacgt tattatctct ttgtaaaaat agccatttat taatcattga
     2281 aaactgcttt tagaacttga tacaacggga ctagtcacaa caggactatt ctcaacggga
     2341 tcatcctcag aggaactatc atcaaagtca tcatccataa ataaaatatc atcgaatggt
     2401 gccacgcccg tgatgagttt tattttatta ttacgatcag tcaagactcc acttaaaccg
     2461 ttttcgctca caggttttaa tgatttttca ttactcttgt tgtaagcagg cgcattaaaa
     2521 atacacggag tatcaagatc aaacaatgac gttccccagt tcacatattg aatatcatag
     2581 ttactgaagt tctgtccaga aaagaagcat cccttaaaat ccaatccacc taaattatat
     2641 aaaccatcct cttctttttg gagagtaatg ttaattctgg ctatctcccg gacaaaactt
     2701 ccttttttgt atttgaatac cgtttcaaga ttttcaccag acagcttgac ttctgggaat
     2761 a
//
LOCUS       DAZOYS010000312         2733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOYS010000312 DAZOYS010000000
VERSION     DAZOYS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.314"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            152..1318
                     /gene="nhaA"
                     /locus_tag="ACHUN9_004156"
     CDS             152..1318
                     /gene="nhaA"
                     /locus_tag="ACHUN9_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0697395.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1384..2283
                     /gene="nhaR"
                     /locus_tag="ACHUN9_004157"
     CDS             1384..2283
                     /gene="nhaR"
                     /locus_tag="ACHUN9_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0697396.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2321..>2733)
                     /locus_tag="ACHUN9_004158"
     CDS             complement(2321..>2733)
                     /locus_tag="ACHUN9_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0697397.1"
                     /translation="EDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDV
                     EYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHE
                     GSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          641 a          607 c          693 g          792 t
ORIGIN      
        1 gccgtcaaaa actcatctca ccgctgatgg cgcaaatcct ttaattgctc gtaaaaaacg
       61 agttattcct acactataat ctgattttaa cgatgatttg tgcggggtaa aatagtaaaa
      121 acgatctatt cacctgaaag agaaataaaa agtgaaacat ctgcatcgat tctttagcag
      181 tgatgcctcg ggaggcatta ttcttatcat tgccgctatc ctggcgatga tgatggccaa
      241 cagcggcgca accagtggat ggtatcacga ctttctggag acgccggttc agctccgggt
      301 tggttcactc gaaatcaaca aaaacatgct gttatggata aatgacgcgc tgatggcggt
      361 atttttcctg ttagtcggtc tggaagttaa acgtgaactg atgcaaggat cgctagccag
      421 cttacgccag gccgcatttc cggttatcgc cgctattggt gggatgattg ttccggcatt
      481 actctatctg gcctttaact atgccgatcc gattacccgc gaagggtggg cgatcccggc
      541 ggctactgac attgcctttg cacttggtgt gctggcgctg ttgggaagtc gtgttccgtt
      601 agcactgaag atctttttga tggctctggc tattatcgac gatcttgggg ccatcattat
      661 catcgcattg ttctacacta atgacttatc gatggcctct cttggcgtcg cggctgtagc
      721 aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc cgcacgggcg tctatattct
      781 tgttggcgtg gtgttgtgga ctgcggtgtt gaaatcgggg gttcacgcaa ccctggcggg
      841 ggtaattgtc ggcttcttta ttcctttgaa agagaagcat gggcgttctc cagcgaagcg
      901 actggagcat gtattgcacc cgtgggtggc gtatctgatt ttaccgctgt ttgcatttgc
      961 taatgctggc gtttcactgc aaggcgtcac gctggatggc ttgacctcca ttctgccatt
     1021 ggggatcatc gctggcttgc tgattggcaa accgctgggg attagtctgt tctgctggtt
     1081 ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg acttatcagc aaattatggc
     1141 ggtggggatc ctgtgcggta tcggttttac tatgtctatc tttattgcca gcctggcctt
     1201 tggtagcgta gatccagaac tgattaactg ggcgaaactc ggtatcctgg tcggttctat
     1261 ctcttcggcg gtaattggat acagctggtt acgcgttcgt ttgcgtccat cagtttgaca
     1321 ggacggttta ccggggagcc ataaacggct cccttttcat tgttatcagg gagagaaatg
     1381 agcatgtctc atatcaatta caaccacttg tattacttct ggcatgtcta taaagaaggt
     1441 tccgtggttg gcgcagcgga ggcgctttat ttaactccac aaaccattac cggacagatt
     1501 cgagcgctgg aagagcgcct gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa
     1561 cccagcgagc tgggagaact ggtctatcgc tatgccgata aaatgttcac cttaagccag
     1621 gaaatgctgg atattgtgaa ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg
     1681 gctgatgcac tttccaaacg cctggtcagt agcgtactta acgccgcagt ggtagaaggc
     1741 gagcccattc atcttcgctg cttcgaatcc acccacgaaa tgctgctgga gcaattaagt
     1801 cagcataaac tggatatgat catttctgac tgtccgatag actctacgca gcaggaaggc
     1861 ctgttctccg tgagaattgg cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca
     1921 gaaaaatcgt tcccggcttg tctggaagaa cggcgacttt tgattcctgg gcgacgttca
     1981 atgttagggc gcaaattgct taactggttt aactcccagg gattaaacgt agaaatcctc
     2041 ggcgagtttg atgatgccgc gttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc
     2101 gttgccccaa cgctttatgc atatgacttt tatgccgata aaactgtcgt agaaattggt
     2161 cgcgtcgaga atgtgatgga agagtaccat gctatttttg ctgagcggat gattcagcac
     2221 ccggcggtac agcgaatctg caatacggat tattctgcgc tttttagtcc agcggcgcgt
     2281 taatcggcac ctcccccaaa gttaaggtgg gggagataga ttagttgtac ataaccacga
     2341 ttttgactcg gctcattatt tgcccgcttg agacattatt tccatatgta cgcgggcgaa
     2401 taaatagagg aatctgatta cttccttcat ggggatgctg aaaagagtag taattgcttt
     2461 taccatactg gatttcattc gtaccatcat tggagagttg aaagcctact tgacccgcac
     2521 cgtcctcact gttttctaac tcatttttta atatgctggt tcctgactcc tgtatcgtca
     2581 ctggttcaaa gtaatattca acatcactgg gttcaaaaac taattcacct tgctcgccct
     2641 ggatgcagtc aaatcttata tgatgaaact gataattttc attattgata cgagacagta
     2701 caaatcgtcc agtgtcgatg gattgatctt caa
//
LOCUS       DAZOYS010000313         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOYS010000313 DAZOYS010000000
VERSION     DAZOYS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.315"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUN9_004159"
     CDS             complement(423..680)
                     /locus_tag="ACHUN9_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697398.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUN9_004160"
     CDS             complement(677..1174)
                     /locus_tag="ACHUN9_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0697399.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUN9_004161"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUN9_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0697400.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUN9_004162"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUN9_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0697401.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUN9_004163"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUN9_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0697402.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZOYS010000314         2701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOYS010000314 DAZOYS010000000
VERSION     DAZOYS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.316"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHUN9_004164"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHUN9_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUN9_004165"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUN9_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0697403.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..>2701
                     /locus_tag="ACHUN9_004166"
     CDS             2354..>2701
                     /locus_tag="ACHUN9_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0697404.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
BASE COUNT          784 a          581 c          593 g          743 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 c
//
LOCUS       DAZOYS010000315         2647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOYS010000315 DAZOYS010000000
VERSION     DAZOYS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.317"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            137..469
                     /locus_tag="ACHUN9_004167"
     CDS             137..469
                     /locus_tag="ACHUN9_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0697405.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            616..1977
                     /gene="yegQ"
                     /locus_tag="ACHUN9_004168"
     CDS             616..1977
                     /gene="yegQ"
                     /locus_tag="ACHUN9_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0697406.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2307..2624)
                     /gene="yegR"
                     /locus_tag="ACHUN9_004169"
     CDS             complement(2307..2624)
                     /gene="yegR"
                     /locus_tag="ACHUN9_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0697407.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT          697 a          688 c          623 g          639 t
ORIGIN      
        1 atcaataagt tggagtcatt accaactgac attatctctc cgccctggca ggcatcagaa
       61 aatcgctcat actttaatcg gtaaacagca cctttagatg ctgttttcga tacgcaattt
      121 caatcaagga gtcattatgg ctggttggtt tgaactcagt aagagcagtg ataatcagtt
      181 ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc accagcgagc tgtatacatc
      241 aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc aagaagaacg
      301 ctatgagaaa aaaacggcaa gtaacggcaa attctatttc aatctgaaag ccgccaatca
      361 tcaaattatc ggctccagcc agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc
      421 ctccgttaaa gccaatggca caagccagac ggtgaaagac aatacgtaat tacgatgccg
      481 ggcgtgggtt gtcggcgtcc ggcgctttac cagatccccc cacagcgcta caatgcccgc
      541 ccttaaagtg ggggcactcc cctaaccgct tcatcaggtg aagcggagct gacctgtcat
      601 cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat
      661 atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttactcc
      721 ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc agctcggcat caatgaagcc
      781 cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg
      841 aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg
      901 tccgatccag ggctgattat gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc
      961 tcggtgcagg ctaacgccgt aaactgggcg acggtgaaat tctggcagca aatgggcctg
     1021 acccgcgtga tcctctctcg cgagctgtcg ctggaagaga ttgaagagat ccgcaatcag
     1081 gtgccggata tggagattga gatcttcgtt cacggtgcgc tgtgcatggc ctactccggt
     1141 cgctgcctgc tctctggcta tatcaacaag cgcgatccga accagggtac ctgcaccaac
     1201 gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgacgttgg caacatcgta
     1261 cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctaggtat cggcgcgcca
     1321 accgacaaag tgtttatgat cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt
     1381 gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgta
     1441 gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggccgtac taaatctttc
     1501 tactattgcg cacgcaccgc acaggtttat cgtaaagcta tcgatgatgc cgctgcgggc
     1561 aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc
     1621 gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca
     1681 gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgca
     1741 gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc ttgagctgat gacgccgcaa
     1801 ggcaacatta actttaccct tgagcacatg gaaaacgcca aaggtgaagc gatgccggtc
     1861 gcaccaggcg atggttatac tgtgtggctc ccggtcccgc aggatcttga gctaaattac
     1921 gcgctgctga tgcgtaattt ctccggggaa accacgcgta acccacacgg taagtgatta
     1981 atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt
     2041 attaaggtta tcatccgttt cgctgaaaaa cataacccat aaaatgctag ctgtaccagg
     2101 aaccacctcc ttagcctgtg taatctccct tacacgggct tattttttat gcataagccc
     2161 tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg
     2221 cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca
     2281 tgtttgatgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg
     2341 ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta
     2401 acacagaaat gggctgcatc attttttact atattttctg caccagatat tctgccccta
     2461 gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt
     2521 ttatatgcaa gaccaaattt accgatactt gtttcatcat tatgcacagc acaaccagat
     2581 ataagaaaaa tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt
     2641 aaatacc
//
LOCUS       DAZOYS010000316         2646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOYS010000316 DAZOYS010000000
VERSION     DAZOYS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.318"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(26..316)
                     /locus_tag="ACHUN9_004170"
     CDS             complement(26..316)
                     /locus_tag="ACHUN9_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0697408.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUN9_004171"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUN9_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0697409.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="ACHUN9_004172"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="ACHUN9_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0697410.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUN9_004173"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUN9_004173"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2646)
                     /locus_tag="ACHUN9_004174"
     CDS             complement(2172..>2646)
                     /locus_tag="ACHUN9_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0697411.1"
                     /translation="PYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREA
                     RRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDAD
                     NQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          697 a          715 c          599 g          635 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgaccatt ccattcaaac
     2461 gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa tttcctgacg tctggcctct
     2521 ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg ctatctcacc ccatttgcca
     2581 ctttgcagtt cctgccagat ttgctgaccc gtcggtgcga catcatcagc ggtggctgtg
     2641 tagggg
//
LOCUS       DAZOYS010000317         2645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOYS010000317 DAZOYS010000000
VERSION     DAZOYS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.319"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..186
                     /gene="hemP"
                     /locus_tag="ACHUN9_004175"
     CDS             <1..186
                     /gene="hemP"
                     /locus_tag="ACHUN9_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0697412.1"
                     /translation="YTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDHDG
                     QEYLLRKTQAGKLLLTK"
     gene            complement(190..1626)
                     /gene="selO"
                     /locus_tag="ACHUN9_004176"
     CDS             complement(190..1626)
                     /gene="selO"
                     /locus_tag="ACHUN9_004176"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0697413.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1689..2402)
                     /gene="ydiV"
                     /locus_tag="ACHUN9_004177"
     CDS             complement(1689..2402)
                     /gene="ydiV"
                     /locus_tag="ACHUN9_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0697414.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT          723 a          680 c          596 g          646 t
ORIGIN      
        1 tatacggata gcagaaaact cacgcctgaa acggacgcca atcacaaggc cgcttccccg
       61 cagcccatcc ggcgaattac cagccagaca ctgttaggtc cggatggcaa actgattatc
      121 gatcatgacg ggcaagaata tctgctccgt aaaactcagg ctggcaagct gctgttaact
      181 aagtagcctt taactcgagc agctgacttc cagccgttta ccccagtcag gtggacgact
      241 gacatagtca tcatctctat cgctgaaagg atttcgcaac gccccatgca ggcggtgcaa
      301 ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt tgcgccaacc agttgcgcaa
      361 taccagagcg gggttaacgc tttgcatcaa ctgctgacgc tcactatcgc taacctcgtc
      421 ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca aatgccgcac gatcaataaa
      481 ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc agactcagca tgcggaatgt
      541 gcgggtataa tcgctgcgct ctcgcgccat cagactgaat aattcattca gtagcgcgtt
      601 atcctctttt tgctccgtga tgaagcccag tttctgccgc atccgttgtc cataatgcgt
      661 caacaaaacc tgctgatagc tgtccagggc ctcattcagg gcatctacgg caacaaatgg
      721 tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg acagcaggtt gattatcaaa
      781 actataacgc ccttgatgat ccgagtgatt acaaataaaa ccgggttcgt aatcatcaag
      841 aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc gacatgttgt cggtattcat
      901 caccccatgt gcaaagccga ccgtctgcca ttgggcaatt aacgacgcgg tacgtgcgac
      961 aacatcgcta aaccagagac ggtatttgtc ctcatcatct tcaagatgtg accagtaatg
     1021 acgaatagca aagtcagcta actgacgaac cttttccgac tcgcggcggt agtaaaaatg
     1081 ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc atcagcatcg cgcctggctc
     1141 cgccgtttcc cgatacactg gcgaatcgct ggtgacgata cttaacgcgc gggtcgtcgg
     1201 aatgcccaga taatgcatcg cctcactggc gagactttct cgtatcgtcg aacgtaaaac
     1261 cgcccgtcca tcacccattc gcgaataagg cgtcaggcca gcacctttca gatgccagtc
     1321 cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa atgccgcgcc catcacccag
     1381 ttggcccgcc cagacgccga actgatgacc actgtaaacc tgcgccagtg gtgacatacc
     1441 aggcagtagg gcttcgccgc cccagacacc tgcgccattt ttaaacagcg acgatggaat
     1501 actcagcgtg ttagccagtt cggtattatg ccaaatcagc cgggcattat ttaaaggcgt
     1561 aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc cagcgggtaa caaaagacag
     1621 ggtcatagat cctcctgttt gatagtgtag acggttactc tcgtcttaaa caccagcaaa
     1681 cagaagggtt atcgctgaac caacgtcgtt atctgattta tgggaacggc aggccacagg
     1741 catccctgga gagcatggaa atcgaagggg gtaatctgtg ccaatatttc tgccgtatca
     1801 atgcctcccg caatgatgca attgcaacaa ggggaaattt gcgcctggat cgcacgaata
     1861 aacggttcga aggagcggtg cgtgatttgt tgctgaataa aacttttatc cagcatgacg
     1921 cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat tgcctgctcc caaattacct
     1981 aacacaaggg gatacacttg cgataaggat aaaagaccgc gattatcttt tccttcattg
     2041 agatgcgggt aattctcatt aatcaacagt tcaataaacg gatattttaa tagttcgcct
     2101 gcgtaattat ccctctctaa taataatgta gccacttgtg gcgttaaatt aagccaggca
     2161 aagagtttat gttgaataaa aaaatgttgg catgatttta gtaattccaa ctgctctgaa
     2221 aataattgcc aatgttgctc ctcggtaagt tgcgctataa cccgactcgt cgggatacgc
     2281 accgtgccat cttcactgga gaaatgggta attaactcaa cgccgacaag gacttgctgg
     2341 ttatctctta tcggcaggaa atagcaatca gaatgataaa gattctccaa aaaaatcttc
     2401 atggtaaccg tccctctcga aggatgtttt tcagtatccg gctaaaaggg atgaggccat
     2461 aagacataac aattacagaa ggagtaactt tcatttgttc catgttaacc actttttcag
     2521 gggtcctttt tttagattat cctgattata aacgaataat ccatttacgg aatttttgtc
     2581 tgcaaaatac tactgtattc aggagtaaat ggacggcgat gtatcatcgc cgtccatttt
     2641 tcgac
//
LOCUS       DAZOYS010000318         2633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOYS010000318 DAZOYS010000000
VERSION     DAZOYS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.320"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            184..1755
                     /gene="garD"
                     /locus_tag="ACHUN9_004178"
     CDS             184..1755
                     /gene="garD"
                     /locus_tag="ACHUN9_004178"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0697415.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1904..2302
                     /locus_tag="ACHUN9_004179"
     CDS             1904..2302
                     /locus_tag="ACHUN9_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFR0697416.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQHSTFNIQHSTFN
                     IQHSTFNIQQGKKLVAGMDVNIDDEIGDDE"
     gene            2302..>2633
                     /gene="yhaV"
                     /locus_tag="ACHUN9_004180"
     CDS             2302..>2633
                     /gene="yhaV"
                     /locus_tag="ACHUN9_004180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0697417.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          710 a          621 c          673 g          629 t
ORIGIN      
        1 cgcgctaaag cacagatttg cgctttacct taccgggcgg cactgcaatc cctgaaatga
       61 ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt
      121 catttatttt taaatgagac cagatcctca ttttaataac ccctggctgg agaatattgc
      181 acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac
      241 gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt
      301 ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac
      361 attccagcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgcgca
      421 atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta
      481 cacacgctgc cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc
      541 tttgagggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc
      601 accagcgtcc actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat
      661 ctgctaccga aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt
      721 ggcgtggcga ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg
      781 ctgaacccta actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag
      841 cctgagcgcc tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc
      901 attgtcagtt tgcaggatga aaagcatgtc ggctttcagt ccatggtcga ggatattttg
      961 caggtcgccg aacgccatct acaaaaactg aatcaacggc agcgagaaac ctgcccggct
     1021 tcagaactgg tcgttggtgt gcagtgcggt ggcagcgatg cgttttctgg tgtaacggca
     1081 aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt
     1141 tcagaagtaa cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa
     1201 gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga
     1261 aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaatgtg
     1321 gtggagaagg cactcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg
     1381 tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat
     1441 tttgtctgtg gcacgcaaca ggtggcttcg ggtatcaccg tacaagtgtt tacgaccggc
     1501 cgtggtacgc cgtacggcct gatggcggta cccgtaatta aaatggcaac ccgcaccgag
     1561 ctggcgaacc gctggtttga tttaatggat attaacgcgg gcactatcgc taccggcgaa
     1621 gagagcattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg
     1681 aagaagaaaa ccttctcgga tcaatggggg ctgcataacc agctggcggt gtttaacccg
     1741 gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt
     1801 ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc
     1861 ttataatttg agcagaaaac aggctgtaaa aggacaatga atcatgcccg ctaatgctcg
     1921 ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc
     1981 cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc attacgaaat
     2041 tctgcctggt gggcaagtat ttatgtgccg actgggagat gaacaggagg atcatactat
     2101 gaatgcattt ttgcgttttc tggatacaga tatccagaac aacccgcaaa aaactcgtcc
     2161 attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca ttcaacattc
     2221 aacattcaac attcaacaag gaaagaaact tgtcgctggc atggacgtca acattgatga
     2281 tgagattggc gacgacgaat aatggatttt ccacaaaggg ttaatggttg ggcgctatat
     2341 gctcatccct gttttcagga aacctacgac gctttagttg ccgaagtcga gacattaaag
     2401 ggaaaagatc ctgaaaatta tcagagaaaa gccgccacaa agttattggc ggtagtccat
     2461 aaagtgattg aggagcatat cacggtcaat ccatcatcac cggcattccg tcatggcaag
     2521 tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa aatttggtgc aggtcgttat
     2581 cgtctcttct ttcgttatag tgaaaaagag aaagtcatca ttctgggatg gat
//
LOCUS       DAZOYS010000319         2609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOYS010000319 DAZOYS010000000
VERSION     DAZOYS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.322"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            93..1706
                     /gene="mppA"
                     /locus_tag="ACHUN9_004181"
     CDS             93..1706
                     /gene="mppA"
                     /locus_tag="ACHUN9_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0697418.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1757..>2609)
                     /gene="ynaI"
                     /locus_tag="ACHUN9_004182"
     CDS             complement(1757..>2609)
                     /gene="ynaI"
                     /locus_tag="ACHUN9_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0697419.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT          747 a          587 c          630 g          645 t
ORIGIN      
        1 ctgtgtcact tttttgatgg aagttgtttg catttcttta aggcgaaaca aataattacg
       61 catcaatttt aatgtcggtt agagggaaac ttatgaagca ctctgtttca gtcacgtgtt
      121 gtgcgctgtt ggtcagcagc atttctcttt cgtatgctgc agaagttccg agcggcacag
      181 tactggcaga gaagcaggag ctggtgcgcc acattaaaga tgagcctgcg tcgctggatc
      241 ccgctaaagc cgtgggcctg ccagagattc aggtcattcg cgatctgttt gaaggactgg
      301 tgaatcagaa cgagaaaggg gagattgtcc ccggcgttgc gactcagtgg aaaagtaatg
      361 acaaccgtat ctggactttt accctgcgcg ataacgcaaa atgggcggat ggcacaccgg
      421 taacggcgca agattttgtc tacagctggc agcgtctggt ggacccgaaa acgctatcgc
      481 catttgcatg gtttgccgcg ctggcgggaa tcaacaacgc acaggcgatt attgatggta
      541 aagctacgcc agaccagctt ggcgtcaccg cagttgatgc ccatactttg aaaattcagc
      601 ttgataaacc gttgccgtgg tttgtgaatt taaccgctaa ctttgccttc ttcccggtgc
      661 aaaaagccaa cgtagaaagc ggtaaagagt ggacgaaacc cggaaatctg atcggcaatg
      721 gcgcttatgt tcttaaagat cgcgtagtca atgaaaaact ggtcgtggta ccgaataccc
      781 attattggga taacgccaaa acggtactgc aaaaagtgac cttcctgcca attaatcagg
      841 aatccgcagc cactaagcgt tacctcgcgg gggatattga tatcaccgaa tccttcccga
      901 aaaatatgta tcagaagctg ttgaaggata ttccggggca ggtttatacg ccgccgcagc
      961 tcgggaccta ttattatgcg tttaacacgc aaaaagggcc gacggcagat cagcgcgttc
     1021 gtctggcatt aagtatgacg atagatcgcc gcctgatgac cgaaaaagta ttagggacgg
     1081 gcgaaaagcc agcgtggcat tttacaccag atgttaccgc cggatttacg ccggaacctt
     1141 cgccgtttga acaaatgagt caggaagaac tgaatgcgca ggcaaaaact ttgttgagcg
     1201 cagctggtta tggtccgcaa aaaccgctga agctgacgct tttgtataac acttcagaaa
     1261 accatcaaaa aattgcgatt gctgtagcat cgatgtggaa aaagaacctt ggcgtagatg
     1321 ttaaattgca aaatcaggaa tggaaaacct atatcgatag ccgtaacacc ggcaattttg
     1381 atgttatccg cgcctcatgg gtgggggatt ataatgaacc ctccactttc ctgacattat
     1441 taacgtcaac gcattcagga aatatttcac gctttaacaa tccggcatac gacaaagttc
     1501 tggcccaggc atcgacggaa aataccgtta aagcgcgtaa tgccgattac aacgcggcag
     1561 aaaaaatcct catggagcaa gcaccgattg caccaattta tcaatatacc aatggacgat
     1621 taatcaagcc gtggctgaaa ggttatccca ttaataaccc ggaagatgtg gcgtacagcc
     1681 ggactatgta tattgtgaag cattgatgtg atgggaactg gcgttaccct tgtggataac
     1741 gccagtgatg tctgatttag cgcccctgtt ccggcggtgt aatgttatcc atatacagcg
     1801 tctggctggg gaaggcaaag tccgcgccgt gtgactgcac aatatcgata atcttcaaat
     1861 aaacgtcttg ctgtgcagca agccattcag cccataccgt ggttttggta aagcaataaa
     1921 ccataatatt caatgacgag tcggcaaact ggttgaaata aaccagtaag gtttgtcgct
     1981 ggtcgatggc cgggtgattt ttcagcatct cacgtacggc ttcgacaata acgcccactt
     2041 ttgccgcatc ctcataacgt aaaccaatgg tcgtggtaat gcggcggttg gtcattcgtc
     2101 ctgggttttc tacgctgatc gacgaaaaca gcgagttcgg tacgtacaat ggacgattat
     2161 caaaggtcgt aattttggta attcgccagc caatttccgc tactgtacct tcgatatttc
     2221 tgtccggtga acggatccag tcgccaatac tgaaaggacg gtcgaaatag agcatgatcc
     2281 cggaaaagaa attactcaga atatctttac cggccatacc gacagccaga ccaccaatac
     2341 caccaaaggt cagcaagcca gaaaggctca tgccgaaatg ttcgccataa agcagaacaa
     2401 gcaccacaat aatggtgatt ttgataatgc gcgacataat ccgcgcactg gtgatatcgc
     2461 gacctttttt aatctgctgt ttttcaaact gattaatcag caaaaatagc ttaatcgtca
     2521 gaataaccgc aatcagggac gtacagataa aatcgataac gtctggggtg ataaatttga
     2581 gtttatagtt ttctataaca taattaata
//
LOCUS       DAZOYS010000320         2603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOYS010000320 DAZOYS010000000
VERSION     DAZOYS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.323"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHUN9_004183"
     CDS             complement(116..868)
                     /gene="tcyN"
                     /locus_tag="ACHUN9_004183"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0697420.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHUN9_004184"
     CDS             complement(865..1533)
                     /gene="tcyL"
                     /locus_tag="ACHUN9_004184"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0697421.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHUN9_004185"
     CDS             complement(1548..2534)
                     /gene="dcyD"
                     /locus_tag="ACHUN9_004185"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0697422.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          620 a          734 c          647 g          602 t
ORIGIN      
        1 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
       61 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
      121 gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatca gcaaataacg
      181 cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
      241 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
      301 gctggcggat tgtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct
      361 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgct acacgctgct
      421 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
      481 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      541 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
      601 caaacccgac gtgctgacgc aactggcgga tcagcgattt ttgctgactt aatgaacgtg
      661 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat
      721 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
      781 cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
      841 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
      901 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
      961 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
     1021 cagctccggc acctggattg tcgcggccag cgacgtatcc tttaccaggc tgataaagct
     1081 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatagt
     1141 ctgccatggc gtcataccaa tactggcagc tgcttcccac tgacctttat caatagaaga
     1201 aatggcggcg cgcagtgttt cggcggcata ggcggcagtg ttcagcgaca gaccaatcat
     1261 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
     1321 cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1381 cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagca gtaagccaaa
     1441 aaacatgccg ccgatactga gttgcagcgt atacccagcc cctttcagca ggaacggcaa
     1501 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
     1561 tgataggcga acagcgcagg cgcaccgccg gtatgaataa acagaatcgg cccttcatct
     1621 ttgaagcgtt tctgactgat acagccaatc agccccgcca tcgcttttcc ggtatacaca
     1681 ggatcaagca gaatgccttc cagccgcgcc agcaatttca ctgcttccat gccttcgtcg
     1741 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtataatttc cgctgatgcg
     1801 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caactgatcg
     1861 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
     1921 ccaacagcca gtccggcgtg agttccggca ctgcccgatg cgactaccac cgacgaaata
     1981 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acctagcgcg
     2041 ccaagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
     2101 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcttcgca catttcaatc
     2161 tgggtattga acagatccag caacaagcga ttgccgttgg ttaaatagtt ttctgcggtt
     2221 gtgccaatag gattttccag cagcgccaca cagtgcagac cgagtttcgc cgcaactgcg
     2281 gcagtctgcc gcacatggtt agactggatc accccggcag taatcagcgt atcggcacct
     2341 tcgcgcagag catctgccgc gagaaattct agcttacgta atttattgcc gcccattgcc
     2401 atgggggtga cgtcatcccg tttgatgaaa atttcccgtc ctagataatc agaaaagcgc
     2461 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
     2521 ttatgcagtg gcataacagc ctccgatgta tgttgttgtg attttcttat tatgcacgct
     2581 gaaaacgcgt aaataaaaaa ggc
//
LOCUS       DAZOYS010000321         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOYS010000321 DAZOYS010000000
VERSION     DAZOYS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.324"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUN9_004186"
     CDS             complement(<1..458)
                     /locus_tag="ACHUN9_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0697423.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHUN9_004187"
     CDS             complement(497..784)
                     /locus_tag="ACHUN9_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697424.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUN9_004188"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUN9_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0697425.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHUN9_004189"
     CDS             complement(1417..2082)
                     /locus_tag="ACHUN9_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0697426.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..>2587)
                     /locus_tag="ACHUN9_004190"
     CDS             complement(2251..>2587)
                     /locus_tag="ACHUN9_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697427.1"
                     /translation="ELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVRVDEKERQ
                     VRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIERNAKARI
                     RRATKSRTE"
BASE COUNT          649 a          672 c          574 g          692 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcaccgcag attttacagc ggacctgacg
     2461 ctctttttcg tcaacccgga cagaggcgtg atggcaatat ggtctttccg atggttcata
     2521 aagaaaatta acctgattac gtgggtcatc ctcttttacc ggaaataaaa cgatattgct
     2581 taactca
//
LOCUS       DAZOYS010000322         2581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOYS010000322 DAZOYS010000000
VERSION     DAZOYS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.325"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            255..773
                     /locus_tag="ACHUN9_004191"
     CDS             255..773
                     /locus_tag="ACHUN9_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0697428.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            983..>2581
                     /locus_tag="ACHUN9_004192"
     CDS             983..>2581
                     /locus_tag="ACHUN9_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0697429.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          640 a          664 c          719 g          558 t
ORIGIN      
        1 cattttatgg aggcaatacg gactttgaac agttgggtat ggttatccag atatatatta
       61 ttaagttatt gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta
      121 aattctagct cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag
      181 ctgcatctgc ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa
      241 caaggagttt acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa
      301 atattactgc aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg
      361 aagacgagat gctggtgcag gaattcctgc ataacgttac tgtcccgacc actcctcagt
      421 ccggtcagcc tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca
      481 aagcggttcc gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac
      541 ttcactggtg gcgtacctct gtggagggca aacaggagca ttatttcact acccgcctga
      601 ctgattcgac gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac
      661 gcgagttcac ccagttgctc gaggtttcac tggcctaccg caaaattgaa tgggaacacg
      721 tcaaatctgg cacctccggt gccgacgact ggcgcgcacc actggaagca taagttaagc
      781 caacagcatc cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt
      841 tgtcagtaag gggttacgtt cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt
      901 gcctgaacgc aagcgtaaca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt
      961 ttccttaacc ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg
     1021 cctgccaccg gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct
     1081 cttttccctc gagctctctc tggtcagcca gcagtttctc tcccttgaat ttccgcaggt
     1141 actggacaaa atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa
     1201 aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagatgc tgtacagcat
     1261 gaaggtatgc ccaccgctgt ggcgcacagg gctgcgccag aacttccgta tcttccagaa
     1321 tgaggacatc gaaagcatcc tcgcgacgat cctgaaggaa aacggtgtga ccgagtggag
     1381 tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg gcgaaactga
     1441 ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg aggagcacgc
     1501 gcaaaaaagt accgaccaga gcctggtcct gtgcgacacc gtgcgttatc tgccggagtc
     1561 ctttgagatc ccctggaacc cgaacacccg taccgaggta agtaccttct gcatcagcca
     1621 gttccgctac agcgcacaaa tccgtccttc ttccgtggtg accaaagact acacctttaa
     1681 acgacccggc tgggcagggc gttttgatca ggaaggccag taccaggatt accagcgcac
     1741 acagtatgaa gtgtatgact accccggacg tttcaagggt gctcacgggc agaactttgc
     1801 ccgctggcag atggatggct ggcgcaacaa cgcagaagtg gcgcgcggaa caagccgttc
     1861 tccggagata tggccgggac ggcgaattgt gctgacgggg catccgcagg cgaacctgaa
     1921 ccgggaatgg caggtggtgg caagtgaact gcacggcgag cagccgcagg cggtgccggg
     1981 acgcagtggt tcaggaacca cgctgaataa ccactttgcg gtaataccgg cagaccggac
     2041 atggcggcca cagccgttgc tgaaaccgct ggtggatggc ccgcagagcg ccgtcgtgac
     2101 gggaccggca ggcgaggaaa tcttctgtga tgaacatggc cgcgtgcggg tgaaatttaa
     2161 ctgggaccgc tataacccgt caaaccagga cagttcatgc tggatccgtg tggcacaggc
     2221 gtgggcaggc accggattcg gtaaccttgc gataccgcgc gtgggtcagg aggtcattgt
     2281 ggacttcctc aacggcgatc cggaccagcc gattattatg gggcgtacct accaccagga
     2341 aaaccgcaca cccggtagcc tgccggggac gaagacgcag atgaccattc gttcgaaaac
     2401 ctataagggc agcgggttta atgaactgaa gtttgacgat gcgacaggga aagaacaggt
     2461 ctacatccac gtgcagaaga acatgaacac cgaggtgctg aataaccgca ccacggatgt
     2521 gataaacaac catgccgaaa aaataggtaa caaccaggcg atcaccgtta ccaataacca
     2581 g
//
LOCUS       DAZOYS010000323         2571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOYS010000323 DAZOYS010000000
VERSION     DAZOYS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.326"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            42..551
                     /gene="eco"
                     /locus_tag="ACHUN9_004193"
     CDS             42..551
                     /gene="eco"
                     /locus_tag="ACHUN9_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0697430.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(860..2506)
                     /gene="mqo"
                     /locus_tag="ACHUN9_004194"
     CDS             complement(860..2506)
                     /gene="mqo"
                     /locus_tag="ACHUN9_004194"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0697431.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          599 a          723 c          648 g          601 t
ORIGIN      
        1 atatgttaac cttcagcgac atcatcggtg aaaacctata aatgaagaag gaaagcaaaa
       61 aaatgaagac cattctacct gcagtattgt ttgccgcttt cgccaccact tccgcctggg
      121 cggcagaaag cgtccagcca ctggaaaaaa tcgcgcctta tccacaagct gaaaaaggga
      181 tgaagcgtca ggtgattcag ttaaccccgc aagaagatga atctaccctg aaagtagaac
      241 tgttaaccgg tcagacgctg gaagtcgatt gcaatttgca tcgtctcggc gggaagctgg
      301 aaaacaaaac gctggaaggc tggggctatg attattatgt ctttgataaa gttagctcgc
      361 cggtttccac gatgatggcc tgcccggatg gcaagaaaga gaagaaattt gtcaccgcgt
      421 atctgggcga tgctggaatg ctgcgttaca acagcaagct gccgatcgtg gtgtatacgc
      481 cagacaatgt agatgtgaag taccgcgtct ggaaggcgga agagaaaatt gacaacgcgg
      541 tagttcgcta aactgctgtg aagtgcgtca ccccgtaggt cggataaggc ggtcacgccg
      601 catccgacat ctaacgccca agccggttgc ctgatgcgac gctggcgcgt cttatcaggc
      661 ctacaccgct gtgaagtgct ccaccccgta ggttggataa ggcggttacg ccgcatccga
      721 catctaacgc ccgagccggt tgcctgatgc gacgctgacg cgtcttatca ggcctacacc
      781 gctgtgaagt gctccacccc gtaggtcgga taaggcggtt acgccacatc cggcatacca
      841 tgccggatgt ggcacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
      901 gtttcaactg cggtttcggc gtactatctg ctgcctgcgg cttgtcgtat ttcaggccca
      961 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
     1021 gtccataaga tggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
     1081 cgccaaatac tttttccagc agatccaaca taatcggcgc agcggttgac gcccctggcg
     1141 atgcccccag cagcgcggca atggtccctt gctggtcact gaccacttca gtacccagac
     1201 gcagtacacc acctttatcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
     1261 acaaacgcca gtcctctttt ttcgcctgcg gatagtactc tttcaacgct tcaaaacgat
     1321 cctcttcact caacatcacc tgactcacca agtatttcac cagatcgaaa ttatccagcc
     1381 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
     1441 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
     1501 taccgtccag aacgcgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
     1561 ctttaccgta aaccttcgcc agatggtgat taaccacgtc cgggttttcc gaaacaagga
     1621 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
     1681 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcacga atgttctgtg
     1741 cagtgccatt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
     1801 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgc aaggaagcaa
     1861 ttaactggcg ggtgatctcg ccgtagttca catcggtacc aatttccgta cgcgtggctg
     1921 ccactttctg ttgcggatcg cgcccttcca tcactaacgg tgcccactct ttgatctgcg
     1981 cgtgatcttc agagtaacgc ataccgcgaa acagcgagct ttgttgcaac gcggcgtaac
     2041 gggcgcgcag gaaattaacg ttatcctcgc cccagacaaa gctcatatgc ggaacggtat
     2101 tgataaatga acgcggagta cgcagcacgc cgcgctcgac ctggtgcgcc cagaactggc
     2161 gggaaatctg aaatgcttcg ttaatggcga ctgctttttc aatactgata ctgccatcgg
     2221 cgttttgcgg ggtgtagttc agttccatca gtgcagaatg cccggttccg gcgttgttcc
     2281 agccgttcga actctcctgc gcgacaccct ccaggcgctc caccatggtc atcgaccatt
     2341 caggctccag ctcgcgtaaa taggtcccca acgtggcgct cataatgccg ccgccaatca
     2401 acagtacatc agtttcctgc tcctcggacg ctttcgcttt tgccgccatc gaaacggcat
     2461 taagccccac ggccatcgag aagagcatgg cagtcacttt tttcatcttg ttaatgcctt
     2521 acttttagtc gctttattgc aggtgagatt tgcgcggcat caacggtaac a
//
LOCUS       DAZOYS010000324         2568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOYS010000324 DAZOYS010000000
VERSION     DAZOYS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.327"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..388
                     /locus_tag="ACHUN9_004195"
     CDS             <1..388
                     /locus_tag="ACHUN9_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR0697432.1"
                     /translation="IENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLG
                     ISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFF
                     LQRHKDFAFKPFFTNFNTIERIENYL"
     gene            385..864
                     /locus_tag="ACHUN9_004196"
     CDS             385..864
                     /locus_tag="ACHUN9_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0697433.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(918..1868)
                     /gene="corA"
                     /locus_tag="ACHUN9_004197"
     CDS             complement(918..1868)
                     /gene="corA"
                     /locus_tag="ACHUN9_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0697434.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2020..2076)
                     /gene="ysgD"
                     /locus_tag="ACHUN9_004198"
     CDS             complement(2020..2076)
                     /gene="ysgD"
                     /locus_tag="ACHUN9_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0697435.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2238..>2568
                     /locus_tag="ACHUN9_004199"
     CDS             2238..>2568
                     /locus_tag="ACHUN9_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697436.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          747 a          589 c          559 g          673 t
ORIGIN      
        1 tatcgaaaat ggcattaatt caactccctc gacaggatac caacctctta tcctgcaaag
       61 cattaaaacc aaattacttt catcaagact aaataaattt aatgacttcg aggacagttt
      121 taatggctta ggaatcagtg ttcatgatat ttcagctcaa aaaatatctc ttttaagttt
      181 tcaaaaatac gctataggat ggagtgcaac aattcatttt gtagcacagg accacttcgg
      241 tcttgatgta actgatatca aaaacaagac ttacagcaaa tatagatttt ttcgcatatg
      301 gttcttttta caacggcata aagactttgc tttcaaacct tttttcacca attttaacac
      361 tatcgaaaga atcgaaaatt atctatgaaa agcaaactcc agaaaattat attatgttta
      421 tttcttctat gctgcatcta taatttatgg acattgcgtc ctgtacagat tttgtataca
      481 tactctgatg ctggcaattc agtctttctg gtcgttgatc atctgccatg gactgacagc
      541 gataaaataa attggtatct gaagcatcag aatgaaatta aaaatcaaca tcccctacca
      601 gaaggtagtt ggcatacctg gtatgtcatt gatatcggta atggcttcac tgactataaa
      661 aaatatatcg aagggccgta tgaagatctc tactgtttcc cgacaattaa gagcaatgat
      721 aattgcatcg tgaaaaatta cttaatggtt attaatgaat acccttatcg taacactcat
      781 attggtatta acgattttac tgagtatcaa ttaacacaag aaaataaaat agagcgagtt
      841 ttcaatccgc atgactttaa ataagacaat ttttagaaat aagccggata aaaaaatagc
      901 tttatccggc actcatatta cagccagttc ttgcgcttaa agtacagata cggtgccagg
      961 cccgcgagga tcataaagat aatcgcgcct gggtagccga aactccactt cagttctggc
     1021 ataaactcaa agttcatccc atagctggaa gcaacgagcg tcggcggcag gaataccacg
     1081 gataccaccg agaagatttt gatgatgcgg ttctgctcga tgttgataaa acccatcgcc
     1141 gcctgcatca ggaagttcac cttctggaac agggattcgt tatgcggcag cagggattcg
     1201 atatctcgca ggatttcacg cgcctgctcc agttgcccac ccggtaaacg cgctttacgc
     1261 accaggaagt tgagcgcgcg ctgggtatcc atcagacaca ggcgaacttt ccagccgata
     1321 tcttccagtt ccgccagagt ggagagcgcc tcgtcgtact catcgccctg atgcccttcc
     1381 ataatcaccc ggctcaactg ctccaggtcg ctatagatat tttcaatttc atctgccaac
     1441 tgttcgattt tggtttcgaa cagatccagc agcagctcgt aggcgttacc gtctaccatc
     1501 gactggctac gggcacgcat acgatacaga cgaaaagcgg gcagttcacg ctcacgcaga
     1561 gtaaacagac gaccatcacg gatggtaaat gccacagtgg agttaccggc gtgatcttcc
     1621 gcatcttcaa agaagaagaa ggagtgaata tgcaggccgt cgtcgtcttc aaagaaacgt
     1681 gccgatgctt cgatgtcttc cagttccggg cgggttgcca ggctctggcc aagttcagat
     1741 tgtacgcgca gtcgctcgtc gtcgtccggt tcgacaagat caatccatac tgcatttaca
     1801 aggggttgtg actcttcgac ttccagccgg gtcagtcggt tattttccag ttgaaatgcg
     1861 ctcagcatga ccgggactcc caatgcttaa aatatcggac agttcggtgg gcacacagaa
     1921 acaaattggg tttcagacca ttaaacagcc tgactcagcg cgacgggaaa aattgaggtc
     1981 gctgacaacc gctaaggcta tcagcaaaaa gggatagcct taggagttga tcctggatga
     2041 caggatagtg agccagtatc tactgggtgt gtccaaggcg aatgtcctct tagagtgatc
     2101 gtgcgcgcat gttacgccag caaaattttg ccgtcaacac gcaacagaac accacagaac
     2161 aatagttgaa cttcgcgctt tttggtactc gtcaaggtcg ctaacaggat aaaaaaatcc
     2221 aacatccagg acctataatg gcgcatcggc tactcattgg taaaggaatg atcaccttga
     2281 atctcaaacg gatttttctc gccctcacct tacttccctt gtttgctgtt gccgctgatg
     2341 attgcgcact ctcagatccg atgctgaccg tacaggcgta taccgttaat cctcaaacag
     2401 agcgggtgaa aatgtactgg caaaaagcca atggcgaagc gtggggaact ttacatgctc
     2461 tgctggtgga tatgaatagt cagggtcagg tgcagatggc gatgaacggc ggcatctatg
     2521 atgaaagcta tgcgccgctc ggtttgtaca tcgaaaacgg tcagcaga
//
LOCUS       DAZOYS010000325         2557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOYS010000325 DAZOYS010000000
VERSION     DAZOYS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.328"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUN9_004200"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUN9_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0697437.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUN9_004201"
     CDS             complement(390..1178)
                     /locus_tag="ACHUN9_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0697438.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUN9_004202"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUN9_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0697439.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUN9_004203"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUN9_004203"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0697440.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="ACHUN9_004204"
     CDS             complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="ACHUN9_004204"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0697441.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgtt
//
LOCUS       DAZOYS010000326         2548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOYS010000326 DAZOYS010000000
VERSION     DAZOYS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.329"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHUN9_004205"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHUN9_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHUN9_004206"
     CDS             829..1674
                     /locus_tag="ACHUN9_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0697442.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUN9_004207"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUN9_004207"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR0697443.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHUN9_004208"
     CDS             1861..2061
                     /locus_tag="ACHUN9_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR0697444.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHUN9_004209"
     CDS             2146..2421
                     /locus_tag="ACHUN9_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697445.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          745 a          557 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttacccac
//
LOCUS       DAZOYS010000327         2544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOYS010000327 DAZOYS010000000
VERSION     DAZOYS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.330"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..786)
                     /locus_tag="ACHUN9_004210"
                     /pseudo
     CDS             complement(1..786)
                     /locus_tag="ACHUN9_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(839..1435)
                     /locus_tag="ACHUN9_004211"
     CDS             complement(839..1435)
                     /locus_tag="ACHUN9_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0697446.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1462..2064)
                     /gene="yadL"
                     /locus_tag="ACHUN9_004212"
     CDS             complement(1462..2064)
                     /gene="yadL"
                     /locus_tag="ACHUN9_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0697447.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2079..>2544)
                     /locus_tag="ACHUN9_004213"
     CDS             complement(2079..>2544)
                     /locus_tag="ACHUN9_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697448.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          707 a          551 c          508 g          778 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatctgttga gttactgttg gatcaaagtt tgtatctgta tagaattcaa
      181 ttgtaagggt ctgataaata ccacctacag cccagtattt acgggtgcta ttcgcattat
      241 tgcaactcca atataacacg tcttcgcttg gattatacca cgaaaaatat tgttctgcag
      301 agtcaccaat gtatatccca ggtgaactaa cgttagttac ggtaccgtaa gctgaccaca
      361 tgtttgaaat taataatgtg taatacaaac caggaactga tgtattaaat aatttatggg
      421 agccatacat tttacctgag tcaaccattg cattttcaat acgcattgcg ctaccacttg
      481 tgctatcact tctgttgcaa taaagaaaac caccaccaga agcggtatct aactgactcc
      541 actgtaattt tacacctgta ggtccaagct gcgtcggttt gtagaaaact aaattatttg
      601 tacttgttgc atatctgaat atgacagtag catcgccatt cggccccgtt tctctccatt
      661 tttgatctga cggaccaacg ccgtaactat tcccaacaat atatcctacg ttatcatcag
      721 tgccagcact cgcggtataa caactcaagc acagaattaa aaaaaatgtc tgaagaattt
      781 cttcattatc actccaccat ctactgctgt aacagtcgca gttatttgtt ttgatctatt
      841 attcgtagta cacatcaact aatatatgac tcattacttg gccagatatg acatccagcg
      901 cgggatcgat tttttgcatt ctggcataaa atttccatga agaattatgc agggcagaag
      961 tcattactgg atacacggaa cgagaatttc ctgctgaact aataacattg aatatattgg
     1021 tcgggtcctg aatggaaaag agaacgacac cgacattttt agccccggtt gcgacttcat
     1081 tggcaaatat ttgccttgaa cctgcagcca tgacaccgct aagggctcta aaatcaatat
     1141 gtaactgatt aagcacatcg ccagttccct gtaatgcaca gtctgatact gtgacagtga
     1201 actcttttcc acctggttga tagctttcag gagtaacatt atctgaaaaa tatcccaccc
     1261 caacgacgcc aagatcaata ttaccctggt tactgattcc gcttttgcaa gtgctgtttt
     1321 ttacattggc aacaaggtca atatcctgcc cagcgatggc tggataggta atgggaaact
     1381 gtagtgacag aaacagaatt attctcttca tcagcttacg ttgagtggga tgcatatata
     1441 gttcctggat ctgtcagtct gttagttata ggttactgta aatgtggcag gtgcagaaaa
     1501 tttacccgca gtgacattgc ttacggtttt atttttttcg acgaccagtc gagcactcat
     1561 cggataataa acaaccgcgt tggctgcatt ggagatagtg acctcttgag atgctggtgt
     1621 cacacaacta aattgtgttg cgctccctgt tgccggagtt tcggtgctcc agacttcaac
     1681 ggcgacggca cttgctttat ctgcggctgt ggatccattt gcgagccccg caccgtcatt
     1741 agcagtacct tcgcatgtgg ctcgcttggt taattttatt tgtgcttttt tattggggat
     1801 acccgcacag tctttgaatt tgagtttgaa tgttttaact ttggtcttgg cattgatttc
     1861 agaaatatat acatcgccaa agtcaacaac agaaatgtcc tgatcagagt catttactaa
     1921 tgtagcggta caagtaccta tttctacggt agtaatcacg gtcagcccaa tgctgtctgt
     1981 tgccgcataa gagggtacgc tgaacaatga ggcagccaaa acgacactgc tggataatcc
     2041 ataacgtaaa tttttaaatg tcatcttaat tatccttctt attgatagat gaaattaaac
     2101 gttgccagtg cacgaaaatt ccccggtacg ccttgccctg catctgtttc acgtagcgca
     2161 acggtcattt caagaccctt atcgggctgc atctcgtctg tgctccagcg tatcttttcc
     2221 gcactgttag gtttaaggaa agtggcatca tcagtagtcc gttttttgaa acccataccg
     2281 atattacttg tcgtcgaaga tgaatcacca gactgcggta taataagctt aggtgagctt
     2341 gatgatgcat ttccggtcag agtggtatca atccaactga tgccactgct gcacccacta
     2401 gcaaccagtt taaaatcagc ctgagattct gtcgttttat tcgcgatctt atccagaccc
     2461 atcttaggga ttctcaatgt gtagttatta ttgccatcat tcgtgacgtt attaccagta
     2521 agtgtgatgt tacaggttgt cgct
//
LOCUS       DAZOYS010000328         2541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOYS010000328 DAZOYS010000000
VERSION     DAZOYS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.331"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHUN9_004214"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="ACHUN9_004214"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0697449.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="ACHUN9_004215"
     CDS             complement(1522..2031)
                     /locus_tag="ACHUN9_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0697450.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(2458..>2541)
                     /locus_tag="ACHUN9_004216"
     CDS             complement(2458..>2541)
                     /locus_tag="ACHUN9_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697451.1"
                     /translation="AGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          642 a          660 c          580 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccctg c
//
LOCUS       DAZOYS010000329         2525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOYS010000329 DAZOYS010000000
VERSION     DAZOYS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.332"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            333..476
                     /locus_tag="ACHUN9_004217"
     CDS             333..476
                     /locus_tag="ACHUN9_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0697452.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            489..698
                     /gene="cspE"
                     /locus_tag="ACHUN9_004218"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             489..698
                     /gene="cspE"
                     /locus_tag="ACHUN9_004218"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0697453.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            864..1673
                     /gene="rlmA"
                     /locus_tag="ACHUN9_004219"
     CDS             864..1673
                     /gene="rlmA"
                     /locus_tag="ACHUN9_004219"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0697454.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1670..2236)
                     /gene="mntP"
                     /locus_tag="ACHUN9_004220"
     CDS             complement(1670..2236)
                     /gene="mntP"
                     /locus_tag="ACHUN9_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0697455.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          673 a          592 c          609 g          651 t
ORIGIN      
        1 ctgttttatg agaaaaagtg atataacttt ttattcattg catagcaaaa aatgtgatat
       61 tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt attgaactat
      121 gttagaaaat acgccagttt aagtatctgc ctgaactggc aaggttaagc acaatgatat
      181 atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat cgagatttgt
      241 gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta tgtgataaca
      301 gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt aaagaagtcc
      361 tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt ggtgcctcat
      421 gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca atctaaggaa
      481 tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct aaaggttttg
      541 gcttcattac tccggctgat ggcagcaaag acgtgttcgt acacttctcc gctatccagg
      601 gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt caggacggcc
      661 agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg atctgaagtg
      721 tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt actatgcttt
      781 atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac acttgcggcc
      841 tttagtatcc tgccggagtt gtcatgtctt tttcctgtcc actttgccat cagcctcttt
      901 cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg gcgaaagaag
      961 ggtatgtcaa tctgctgtcc gttcagcata aacggtctcg tgatccgggc gacagcgcgg
     1021 aaatgatgca agcacgccgc gcattcttag atgccggaca ttatcagccg ctgcgtgatg
     1081 caattgtcgc ccaactgagg gaacggcttg atgataaggc cacggcggtg ctggatattg
     1141 gctgtggtga agggtattac acacacgcat ttgccgatgc gttgcccgaa atcaccacgt
     1201 ttggtctgga tgtttcgaag gtagcgataa aagcggcggc gaaacgctat ccgcaggtca
     1261 ctttttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg gacgccataa
     1321 tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag cccggcggct
     1381 gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg ctgatttaca
     1441 atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca ttacagcaga
     1501 gtgcggagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca ttattgcaga
     1561 tgacgccgtt tacctggcgt gcgaagccag aagtctggca aacactggca gcaaaagaag
     1621 tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat taaccgtgga
     1681 agtgcgtcca gaggatctgg acgccgatgc cgatcagtac cagcccgccg agaatttccg
     1741 cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat gttgacataa
     1801 tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc aggaaagcaa
     1861 gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt accagtagcc
     1921 agaaaccgtg tcgacggcgc ggctcttcat cttcatcatc tgcgccacga aaaccctcaa
     1981 taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtgg ttccattcaa
     2041 ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc agggtttcga
     2101 cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta tggagggtgg
     2161 cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca agaagaacag
     2221 tagcagtgat attcatgaca atgtcctgac cggggtatcc ataaaacaca tcatcgcccc
     2281 cagcaaacat gcatacatga acaacaagcc gtgatggttt tcgcgttcat gcaggcatag
     2341 cgattgatgt gcatatggtc tcgcctgact gaaagtattc agtccgtacg tgccacgttt
     2401 tgcaacgagt atgttgacac gtacatttcc ggaatctgga aatcggctac tccccaaatg
     2461 agggcgcaac cttaacatat tttgagaata taaaacaact gttggaatat attatttcat
     2521 tgctt
//
LOCUS       DAZOYS010000330         2509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOYS010000330 DAZOYS010000000
VERSION     DAZOYS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.333"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            118..1071
                     /locus_tag="ACHUN9_004221"
     CDS             118..1071
                     /locus_tag="ACHUN9_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0697456.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1112..2107)
                     /gene="pgl"
                     /locus_tag="ACHUN9_004222"
     CDS             complement(1112..2107)
                     /gene="pgl"
                     /locus_tag="ACHUN9_004222"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0697457.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2403..2498)
                     /locus_tag="ACHUN9_004223"
                     /pseudo
     CDS             complement(2403..2498)
                     /locus_tag="ACHUN9_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          687 a          623 c          569 g          630 t
ORIGIN      
        1 aatgacttta gctcaacgat taataacatt ttttcattaa tttaagttgg ctaaatgcag
       61 ttaaaatttt taacggccag ccacccaaaa tcgtcgcttc cattttccgg agtaataatg
      121 aagcatgaat tatcaagtat gaaggcattt gtcatactgg cagagtccag ttcgtttaac
      181 aatgccgcta aattactcaa tattacgcaa cctgcattaa cgcgaagaat aaaaaagatg
      241 gaggaagatt tacatattca actatttgag cgtacaactc gcaaggttac gttaacaaaa
      301 gcaggaaaaa ggttgctccc ggaggcgcgg gaattaataa agaaattcga tgaaacgctt
      361 tttaatattc gtgatatgaa tgcttatcat cgtggtatgg tgacgttagc gtgtattccg
      421 accgcagtat tttatttttt accgctggca atcggtaaat ttaatgagct gtatcccaat
      481 attaaagtgc ggattctgga acaaggcacg aataattgca tggaatcggt gctgtgtaac
      541 gaatcagact ttggcataaa catgaacaac gtcacaaatt catccattga ttttacccca
      601 ctggtcaacg agccgttcgt gctggcctgt cggcgtgacc atccattggc caaaaagcag
      661 cttgtagaat ggcaggagtt agtgggttac aaaatgattg gcgtacgctc ctccagcggc
      721 aaccgactgt tgatagaaca acagctggcc gacaagccct ggaagctgga ctggttttac
      781 gaagtgcgtc atctttcgac gtcattagga ctggtagagg cgggactggg gatttcggcg
      841 cttcctggtc tggcaatgcc ccatgcgccc tattcttcga ttattggtat tccactggtg
      901 gaacccgtta tacgtcggac attggggatt attcgccgca aagatgccgt gctttctccg
      961 gcagcagaac gcttttttgc cttgctgatt aatttgtgga ctgacgataa agacaatttg
     1021 tggaccaata tcgtcgaacg ccagagacat gcgttacagg agataggtta atacaccggc
     1081 gcaggagaga ctgcgccggg taaatcagcg gttagtgtgc gttaaccacc acccacattg
     1141 gtccctgccc gaccgcatag cggccttttt catgcagtag cccctgctcg ccaacaattt
     1201 cgtataccga gatgtggtga gatttttgcc cggcggcaat cagatacttg ccgctgtgat
     1261 caacattgaa gccgcgcggc tgggtttccg ttggctggaa gccttcttta ctcaacacgc
     1321 tgccatcttc cgaaacgctg aaaacggtaa tcagactggc ggtacggtcg caggcgtata
     1381 aatgacgacc atccggggtg atatgaatat cagccgccca acaggtgtcg gagaagtttt
     1441 ccggcatcat atccagcgtc tggacacatt caatattgcc gtgtggatct ttcagttccc
     1501 agacatccac tgagctgttt aactcattga cgcaatacgc atattgttcg tttggatgga
     1561 ataccatatg acgcgggccg gccccttcaa cggtagtcac ttccgcaggg tcctgcgcca
     1621 ccagatgacc atcatcgctg accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa
     1681 cccacagcgt acggttgtcc ggtgagatat tggcggaatg gcaaccgtcc agcccctcga
     1741 ccacatcgac gacgcccact ggcaggccat cttccagacg cgttacgctc acgttacccg
     1801 cattgtaaga acctacaaag acaaactgcc cctggtgatc ggtggaaata tgcgtcggac
     1861 tacccggcag cgcagactct gcggcaaagg tcagtgcgcc atcgtccggg gcaatacgat
     1921 acgccaggac gcgaaactca gggcgaacac caacatagag ataacgtttg tccgggctga
     1981 ccaccatcgg ctgcacctgc cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt
     2041 catgattcag gttccagacg tgaatttgct ggctctcagg gctggcgata taaactgttt
     2101 gcttcatgaa cacacctcaa aaatgataaa tcccttatct ttcatacaca taacaacact
     2161 tactggataa aaaacagaca acaaaacacg ataaaataca atatcgcaca tcaattaaat
     2221 caataagtta gcactccatt tcgaatggca tattactaaa atattagtca ccgacacgaa
     2281 tttgacaatg ccacagatat tttcgcgact attcagggag tatgactctc cccgcttttg
     2341 tttacctgta tcaacgaccg taaacactat gccataacaa cttctggcca acttcccatt
     2401 cgctcaacaa tcaactcact tccggcacaa ataaacgcat tacgttgtag atcggtattc
     2461 tggtcatttg ttgatacgcg tacatagcca ataagcatgg taaatcccc
//
LOCUS       DAZOYS010000331         2448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOYS010000331 DAZOYS010000000
VERSION     DAZOYS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.334"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..295)
                     /locus_tag="ACHUN9_004224"
     CDS             complement(<1..295)
                     /locus_tag="ACHUN9_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0697458.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWS"
     gene            complement(310..1056)
                     /locus_tag="ACHUN9_004225"
     CDS             complement(310..1056)
                     /locus_tag="ACHUN9_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0697459.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1063..1371)
                     /locus_tag="ACHUN9_004226"
                     /pseudo
     CDS             complement(1063..1371)
                     /locus_tag="ACHUN9_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1750..2262)
                     /locus_tag="ACHUN9_004227"
                     /pseudo
     CDS             complement(1750..2262)
                     /locus_tag="ACHUN9_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2275..>2448)
                     /locus_tag="ACHUN9_004228"
     CDS             complement(2275..>2448)
                     /locus_tag="ACHUN9_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697460.1"
                     /translation="ELRHRLCIFDTLERRALLAAQEALSTAIDAHDDAVQAVYRKAHF
                     SGGGSSDDSVIVH"
BASE COUNT          555 a          723 c          540 g          630 t
ORIGIN      
        1 atgaccagat aaggtcagga tcaaccacaa ccggtttctt cacctttacc ctcgatagct
       61 ttttgcgggc gttttgccag tctttatgag cctgttcaga gggaaatacc ccgtagccag
      121 aattatatac atcaccactt gcgaccagtt ccatgcataa gcgaccgaca gacgcacgac
      181 tgatacctgt ttcatctgac aactgccgaa tcgtgacccg accgtcctga tgcacgagtt
      241 ccacaattcg cgtcttcagt tcttcccgct gttcgggagt aaaaggtttc gccataaatc
      301 ctcctgaaac tacttaacaa ccctcgaatg gctaacattc ggacgccagc tctcccagtt
      361 aaaattcacc cagcgcccgc cgttcatgac catgcgatcc atcacccgct cgccgagaag
      421 tgtattcatc gctgcatggt taatatttgt cagcatcccc acaccgcgta acgatgccgt
      481 ccggcgatca acaatctggt tcagtaccac ctgctcgttt ttcgtatccc gctgcatgcc
      541 aatttcatcc aggaccagca ggtcaacacc acaaagctcc tgtaaaaatt tttcaccgga
      601 tttgccgttg tcgtagctct catgcaacac gctcatgaca tcggacacgg tgacgataat
      661 cacgctacgc cccttcgcca tcagccggtt gccaatcgcg tctgcaagat gattttttcc
      721 ggtgccgggc ttaccgctga acacaaaatt cgtacagcct gtcatcagtt cgtcagctat
      781 ggatttcgcc tgactcagtg catgtcgctg accgtcgttc tgcgcccggt aattcgcaaa
      841 cgaacacttc cggtgcaacg gctggatgcc ggagcggttc aggatttttt ccacccgcaa
      901 ctgacgattc aggcggttga tctcctcgca acgtttctgg ccttcagcaa gttgccactc
      961 gcgccactcc gcaaccgttc tgaatggggc ggttacatgt ggtggggtca gtctgcggat
     1021 acgctccaga acgcctcctg tcgcaatatt tttcatggtc tgttaccccc tgaagcctgg
     1081 cgggatcgca ctgtccggca acgagacggt gttaacctgt cggagcaacg tctcaggccg
     1141 aacacctttc ggcgcgaaca gaccctggta ttcattggcg atgctgtgtc gaatcacctg
     1201 ctcaggtgta aaaccctgct gacggaattt ttccagttcc cgtatcgccc cgttagcgcc
     1261 ctgctccgtt cgaatcggtt ttcgcaatgc ctgcctgaac tggacccact catgccagag
     1321 tgtttctggc aaccagtcgg gcagatcaac tgacaacggg tcaaattttt tctggcgaac
     1381 agccccaccg ataaccagaa ttccctgctc ggtgtgcctt gcgatatcac tgcgccgcaa
     1441 gacacatatc ccggtttcaa cagcaccgat tttgagcagt gatttctgtc gcggagaatt
     1501 tcggtcaagc tcgctcccac accggacacc gttcctgtca gtcacgacga tatcaatccg
     1561 ccctccgcaa ccgtcaccac gctctggcac cggatactca cgacagacat ccaggccagc
     1621 agcctgcaac gcaaccaccg cagaattaca aaattccgct gcagtaccgc cagataatct
     1681 cccctcaagc actccagcca caaaatcccg aaactccctt ccggaaggga agggtttggg
     1741 atgggttaga tctgttttta gatctttatc tgtatcttta ttagttgcct ttgtgttggc
     1801 atcatgttcg aacatcactc caacatctgt ttgaacacct gttaaatttc tctcttgttt
     1861 tgtttgaaca tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc
     1921 tgattttttg gttaattttt ctctgacaga tgccagatct tcctcaatcc gaagatgcat
     1981 ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat ggttcaacat caacccatcg
     2041 ctcgttagtc agacgggcaa tttttgccaa cctgtttttg ggtattggct ttcctgtttg
     2101 ccagtaattg aacatcagca acaaatacgc gccgtgctcc tctgctgaca aatgcatggt
     2161 gtcagccagg taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc
     2221 gtatgatgct gcccggttgc ttagaataag cacaaacagc atggaaactt ttgcttaatg
     2281 aacaatgaca gaatcgtcgg aagacccgcc gccgctgaaa tgcgctttcc ggtaaacggc
     2341 ctggactgca tcatcatgcg catcaattgc cgtactcaac gcttcctgcg ccgccagtaa
     2401 tgcacggcgt tccagggtat cgaagatgca gagtcggtga cgcagctc
//
LOCUS       DAZOYS010000332         2443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOYS010000332 DAZOYS010000000
VERSION     DAZOYS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.335"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            36..1136
                     /gene="ompC"
                     /locus_tag="ACHUN9_004229"
     CDS             36..1136
                     /gene="ompC"
                     /locus_tag="ACHUN9_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0697461.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1300..1782
                     /locus_tag="ACHUN9_004230"
                     /pseudo
     CDS             1300..1782
                     /locus_tag="ACHUN9_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1897..>2443
                     /locus_tag="ACHUN9_004231"
     CDS             1897..>2443
                     /locus_tag="ACHUN9_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0697462.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          798 a          491 c          453 g          701 t
ORIGIN      
        1 aatacgttta acgataatta aaaggattct gttaaatgaa attaaaaata gttgcggtgg
       61 ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat aacaaggatg
      121 gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact gatgataagc
      181 gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg caaatcaatg
      241 atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac gatgaagcca
      301 acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt agtgaatttg
      361 gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca tggaccgata
      421 tgttgccaga atttggtggc gatacctgga gtcagaaaga tgtcttcatg acataccgta
      481 ccaccggtct agcaacctat cgcaactacg atttctttgg cttaattgaa gggctgaact
      541 ttgccgcgca atatcaaggc aaaaatgaac gtactgacaa cagtcatctt tatggtgctg
      601 actacacgcg tgccaacggt gacggtttcg gtatctcctc aacttatgtt tatgatggct
      661 ttggtatcgg tgcggtatat accaaatccg atcggacaaa tgcgcaggaa agagccgctg
      721 ctaatcctct caatgcctcc ggtaagaatg cagaactatg ggccacaggt ataaagtatg
      781 atgccaacaa catctacttt gcagctaatt acgctgaaac attaaacatg accacctatg
      841 gggatggtta tatctctaac aaagcacaaa gttttgaagt ggtggcgcaa tatcaattcg
      901 acttcggctt gcgcccatca ctcgcttacc tgaaatcgaa aggcagagat ctgggccgct
      961 acggcgacca ggacatgatt gagtatatcg acgttggtgc gacgtatttc ttcaacaaaa
     1021 atatgtcgac ctatgttgat tataaaatca acctgattga tgaaagcgac tttacccgtg
     1081 ccgtagatat tcggaccgat aacatcgtcg ctacgggcat tacctatcag ttctaacgtc
     1141 ctctcctgac cagcccttct ctgggctggt ttttgattta agtgctgctg tatgactcct
     1201 taagtaaaaa tgagcaattt ttgatatttc tggtcattca cctcaattaa tattattcca
     1261 tatcctccgg ggacatctta ctataatatt tattgatgag tgattatgac caatattaat
     1321 acagcttgtg taaaaaataa tgccagttat caacttaata acgcattacc taacaaagaa
     1381 accatctcca gcaatttttg tgaacgacta gaacaatggg gtaataaatc ggttaataat
     1441 ggtgaagaaa gagcaattgc cgtggagcga attaaagaag cttacaattc gaacatggca
     1501 tctcttgatt tgtcatatct tgatttgagt gaattaccgc ctatcccctc gacagttaat
     1561 acgttaaatc tggaaaacaa ttgtctaact tgtcttgact tagctgacaa tgccagtctc
     1621 gttaatatca acctcagcgt taataaaatt aacacgataa cctttccaaa agaatcaaaa
     1681 ctggaaaaca tttatattga tcataacaat ctggaaagtt tagatttaaa aaatcagcat
     1741 tcattggtta atctggaagc gcaaaataac aaactgacaa aaattaatat ttctgatagt
     1801 tataaactga aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg
     1861 caagaatctc tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac
     1921 aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc
     1981 aaaaacacta caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat
     2041 gacattgatc aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac
     2101 aatgatccac ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact
     2161 aatgaagtga ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa
     2221 aacttcacat ctatcaatct tgataataac caactcacac attttgatgc aaccaactac
     2281 gatagactcg taaagctaag tctgaatagt aatgctcttg agtcaataaa ttttcctcaa
     2341 ggcagaaatg taagtattac acatatatct atgaataata atgctctcag aaatattgat
     2401 atagataggc tttcatcagt tacttatttt agtgcggcac ata
//
LOCUS       DAZOYS010000333         2410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOYS010000333 DAZOYS010000000
VERSION     DAZOYS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.336"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>2410
                     /locus_tag="ACHUN9_004232"
     CDS             <1..>2410
                     /locus_tag="ACHUN9_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0697463.1"
                     /translation="TDTFAETAAYCDAFEYYANGAISYQKTYKDNIENICQSFGGIMY
                     VHAGQICITTDRKSVSVASFDERNMVGAVQVSTSGGTDYFNVVDCKFTNPESMYTTDV
                     VRIPSDITTDEAVQHDGQVIALSRDYSWSYDQEVIAKMANVDVLKAKYALRTISFTTS
                     EGWDLEVWDAINVSNEELAISGKFKVLNKDISTDQENVGYVTITAVEAPDAMYDGIDP
                     GIWSPGGVINFPELQVLPPTNLQAVRKGNTTSGSIVDLTWDASPDPYLRGYYVYYRLN
                     GSSTWTYAGQTGVQKLDYELFGLSDTASYDFSVEAYNNLGLVSKKLSLTGIVPTFNFA
                     LPAVTRVVLTNKTESAYVTDSPDFNIAWDSQKDIRVNNRSFSEYFKYYVIKIYDGTKL
                     VDTFYTQSNAFNFTLEMNRLKVRKPTIGIIAQGFNDGTYSEEVKITVENKQCGLVEDV
                     SFTGGFGNLFASWTKSTERDYAGAIISIVNGTNTRIFTSYAPEFDSIPNITDGEYKVK
                     MGFFDVFGTDDIQYCPEQTISINSKYQFTEDDAEAINGILDLEDRLTGTINDAVKIAN
                     KNTSTVISASEARTNDKITASEKTLSTQITGVNSSLSQKLSTVESTANGNKASIQTLS
                     QTVTDNNKAQTTAVSQLRSDVNGQIATVNQSMSTKADKSTVDAQYSLSVQANGTVAGM
                     RLVASSGTSNNSAIYFAANKFIVSGTDTATVGGTAPFAIVNGTTYLKTAMIQAASIGT
                     GYIADAAITNLKLASGSVNTLNVVDGCITTAKISNTIQSTNYVANKTGWQINKAGTIL
                     INGTG"
BASE COUNT          746 a          481 c          526 g          657 t
ORIGIN      
        1 accgatacgt ttgcagaaac cgcagcatat tgtgatgctt ttgaatatta tgctaatggt
       61 gctatcagtt atcaaaaaac ctataaagat aatattgaaa atatctgtca gtcatttggc
      121 ggcatcatgt atgtacatgc cggacaaatc tgtattacca cagaccgcaa atcggtatcc
      181 gtagcctcat ttgatgagcg taacatggta ggggcggtac aggtttctac ttctggcggt
      241 actgactact tcaacgtagt cgattgcaag tttactaacc ctgaatcgat gtacaccact
      301 gacgtagtac gtatcccttc cgatatcact acggatgaag cagtacagca tgatggacag
      361 gtcatagcat tatcacgcga ttactcatgg tcatatgatc aagaggtgat tgcaaaaatg
      421 gctaacgttg atgtactcaa ggctaagtat gccctgcgta ctatcagctt caccacaagt
      481 gaaggttggg atctcgaagt atgggatgcg atcaatgtca gcaatgaaga actcgctatc
      541 tcaggcaaat ttaaagtact caataaagat atcagcaccg atcaagagaa cgttggctat
      601 gtaaccatca cggcagtaga agcacctgat gcaatgtatg atggtattga tccgggtatc
      661 tggtcgcctg gtggggtaat taacttccct gaactacagg tactaccgcc aacgaaccta
      721 caggcagtac gtaaggggaa tactacttct ggttcaatcg tggatcttac ctgggatgct
      781 tcaccagatc cgtatctgcg tgggtactat gtttactacc gtcttaacgg ttccagtacc
      841 tggacgtatg cggggcagac tggcgtacag aagcttgatt atgaattatt tggattgtct
      901 gatactgcaa gctacgactt ttcggtagag gcttacaaca acttagggct ggtatccaag
      961 aagctttccc taactggcat tgttccaaca ttcaactttg cactacctgc ggtaacgcgt
     1021 gtagtactga caaataaaac agaatcagcg tatgtaactg actcaccaga tttcaatatt
     1081 gcctgggatt cacaaaaaga tatcagagtg aataaccgat catttagtga gtacttcaag
     1141 tattacgtta ttaaaatcta tgacggtact aaattagtag atacattcta tacgcaaagt
     1201 aatgccttta atttcactct tgaaatgaac cgtctgaaag tacgtaagcc taccattggt
     1261 attatcgcac agggatttaa tgatggtaca tattcagagg aagtgaaaat aaccgtagag
     1321 aataaacagt gtggtttagt agaggatgtt agctttacgg gtggttttgg taatttgttt
     1381 gcttcatgga ctaaatctac tgaacgtgat tatgcaggtg cgattatcag tattgttaat
     1441 ggtactaata cacgtatatt caccagttat gcacctgaat ttgattcaat accaaacatc
     1501 actgatggtg agtataaggt taagatggga ttctttgatg tgtttggtac tgatgatatt
     1561 caatattgtc cagaacaaac aatcagtatc aactccaagt accagtttac cgaagatgat
     1621 gcagaggcaa ttaacgggat tcttgattta gaagatcgcc tgactggtac cattaatgat
     1681 gcggtcaaaa ttgccaataa gaataccagt actgttatca gtgcatcaga ggcacgtacc
     1741 aatgataaaa tcactgccag tgagaaaacg ttaagtactc agatcactgg ggtaaatagt
     1801 tccttatcac agaaacttag caccgttgaa agtactgcca atggtaacaa ggccagtatc
     1861 cagacactat cccagacggt aacggataac aacaaagctc agactacagc agtctcacag
     1921 ctacgtagtg atgtgaatgg acagattgcc actgttaacc agtcaatgag tactaaagcc
     1981 gataaatcta ctgtagatgc tcagtactca ctatcagtac aggctaatgg cacggtagct
     2041 ggtatgcgtc ttgttgcgag tagtggaacg agtaacaact cagcaatcta tttcgctgcg
     2101 aacaagttca tagtctccgg tactgacact gctactgtag gtggtactgc accatttgcc
     2161 atagtcaacg gtactacata cttgaagacg gcaatgatac aggcggcaag tatcggtact
     2221 ggttacatcg ctgatgcggc aattaccaat ctaaaactgg cctccggctc agtgaataca
     2281 ttaaacgtgg ttgatggttg tataactaca gccaaaatct caaatactat tcaaagtact
     2341 aattacgtag caaataaaac aggctggcaa atcaataaag caggtacgat cctaatcaat
     2401 ggaacgggtg
//
LOCUS       DAZOYS010000334         2399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOYS010000334 DAZOYS010000000
VERSION     DAZOYS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.337"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            95..577
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004233"
                     /pseudo
     CDS             95..577
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(800..1156)
                     /locus_tag="ACHUN9_004234"
     CDS             complement(800..1156)
                     /locus_tag="ACHUN9_004234"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0697464.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            complement(1217..1420)
                     /locus_tag="ACHUN9_004235"
     CDS             complement(1217..1420)
                     /locus_tag="ACHUN9_004235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697465.1"
                     /translation="MRVSELPDYLRHHWPELKAQLLSGRYRPSPVRRVSILKPGGGER
                     LLGIQMVVDRFIQQAMMQVLQAL"
     gene            1446..2228
                     /locus_tag="ACHUN9_004236"
                     /pseudo
     CDS             1446..2228
                     /locus_tag="ACHUN9_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          545 a          663 c          627 g          564 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagaa
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgta gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccattaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cgctgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ctgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgct
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tgataggcaa ttcaggtaat aaaaaagctg gagctccctt tcctgcacgt ctcattgccg
     1861 tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg actgctcagc gagaatcgtc
     1921 gaaaaggacg agtagttcag gcggaaacgc tggaggcagc gggccatgtg ctattgctaa
     1981 catcattatc ggaagatgaa tattcagcag agcaagtggc tgattgttac cgtctgcgat
     2041 ggcaaattga actggctttt aagcggctca aaagtttgct gcacttggat gctttgcgtg
     2101 caaaggaact tgaactcgcg aaagcgtgga tatttgctaa tctactcgcc gcatttttaa
     2161 ttgacgacat aatccagcca tcgctggatt ttccccccag aagtgccgga tccgaaaaga
     2221 agaactaacg cgttgtggag aataacaaaa atggtcatct ggagcttaca ggtggccatt
     2281 cgtgggacag tatccctgac agcctacaaa acgcaattga agaacgcgag gcatcgtctt
     2341 aacgaggcac cgaggcgtcg cgttcttcag atggttcaac ccttaagtta gcgcttatg
//
LOCUS       DAZOYS010000335         2376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOYS010000335 DAZOYS010000000
VERSION     DAZOYS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.338"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..318)
                     /locus_tag="ACHUN9_004237"
     CDS             complement(<1..318)
                     /locus_tag="ACHUN9_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697466.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNN"
     gene            955..1506
                     /locus_tag="ACHUN9_004238"
     CDS             955..1506
                     /locus_tag="ACHUN9_004238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697467.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
BASE COUNT          720 a          459 c          520 g          677 t
ORIGIN      
        1 attgttaagg ttgcatatat tttcatatgt tttcaacatc tccaacgaaa aaagatcgca
       61 attagtccag aacttagtaa cttgatttaa agcattttcc tttattttag tttgaaacca
      121 atttttagcg ttccatgctg catatagggc tgctccagcc ataatgagat tggcaataga
      181 actaacccag tcactaaccg atccccactc gaaaccttca gaaccaacaa agagtgattt
      241 tattagtact aaaattacaa caaccagtaa tactgctacg gtcgataaaa gaacgatatt
      301 taattttcta caatccatcg aaagtagttc ctgaacgcat taaaggggca aatactttac
      361 catccgaaac acaatcaagt acactacccg ttcacgtact gctcacatgt tgggtttgtt
      421 attttgttgg ggtgagcagt acgtgtatcc tcagaagggt agttccccct gatcacagaa
      481 ccaatcacct tcttcactgc tttgattcat ccggtactca gcagatagat atgctaaccc
      541 atcaataaca cttattagtg gtgtttcaca ggtaagtaca aaacaatctc tctctatagt
      601 acctaaccag tattccctgt actgattctg gaagaatacc ctatccccat aatgaaattc
      661 tgttagtgca gatccccaat aacacacggt tgcccagcga ccgttatgtg agaacttctg
      721 aacccctcgc gtaatggatg gatcatagcc gtctttgctc atttgttacc ctcctctatg
      781 tattactgta tatatgtaca gtattgcagt cagtttccca aaaatccagc acccctttgt
      841 ttaccacttt gaatgattaa ttctattgta atgatcaata aaattgatcg atttaattgc
      901 caatgtttta aaaatgatca attatcaata acttaaagcg taacgagcaa agttatgaac
      961 attgataatt gggcaatacc gataacaagt ttgggtggtc agttgctggg ggttgagttg
     1021 gtcagccatg tagaacgcga gggaatcagg cagatcggcc tatgggaaga agaagtactt
     1081 catgggcaac taatgcttat tgaaagcaag gcggcatggt tcagggataa cggtttgtat
     1141 tgtgtgatta ctactggccg tggtgaacca tacgagtact taccattcct tcggcagatg
     1201 acaagggata tgaaagtaca taaccagcag tggcttgatg atctcggtgt acatggtggt
     1261 tctgctattc ctctggttac aggtgatttc gaggttgccc gattaaatag aaggtacaca
     1321 gaagagaaca ttgaccgtct gatctttcca gtactcataa aaagcattcg ccagtactgc
     1381 gagaaggtga tcgtacccgt cagagataaa acgtattacc caattctcag agaggccggt
     1441 gtatgggcgg tacagggaga gttcaggcca atgcgttttg agcaatgtga aaaattaata
     1501 ggataacaat aatgaaaaaa ttactaattg ggttactgat tgcccttagt actggatgta
     1561 ctactacgca cagcttcatg gataaagctt acgttggcta tgtagatgac aaggccgtag
     1621 tactgtttga tgcaggaaca cgtaatgggg tgaagtacta ccgtcctcag ttccagagtg
     1681 attgcggtcg catctataac aagatctatc atgatgacaa tacaagtact ggatgcctca
     1741 tacgagattc ccgcattgct gaactacatc cctatagaga gcatgtaagt aaggtagtac
     1801 cacatcagac cgatagtgtt cacgattggt tagaaggggt caattcacca tcccatatcc
     1861 tcgacaatct atagggtacg tatgggaaag gtaatggtga ttttgttagt actggctggg
     1921 gtactcttca acattgcagg tgaggatgca ccccagccac agtacacgga caatgagaac
     1981 cagaacgcta tggatttcgc acagtcacta atcaagggta agtacccatg cgatatggtg
     2041 actatggata gtactgagaa tggtacagga cgtattgatg tacggtgtga tggtaagtac
     2101 cagtatgaga tcatccggca tgatggacaa tggcaggtag tatcgaagta atccgaaaag
     2161 tttagtgctt aggctgtatg gtgagaatct acagggcttt ttctttatgc ggtgataaga
     2221 tcggagtgaa ctttttatga aggaatagtt agtagaatga aaattacagt cgataatatc
     2281 tctgaaatgt ttgcaggaac taaagaacta ctaatcggtg gcttaataac aggtatcgtc
     2341 ggagtactta ttttaactcg tgttagtggt tatgaa
//
LOCUS       DAZOYS010000336         2358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOYS010000336 DAZOYS010000000
VERSION     DAZOYS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.339"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(94..303)
                     /gene="fumD"
                     /locus_tag="ACHUN9_004239"
     CDS             complement(94..303)
                     /gene="fumD"
                     /locus_tag="ACHUN9_004239"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0697468.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            658..852
                     /locus_tag="ACHUN9_004240"
     CDS             658..852
                     /locus_tag="ACHUN9_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697469.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            860..2272
                     /gene="pykF"
                     /locus_tag="ACHUN9_004241"
     CDS             860..2272
                     /gene="pykF"
                     /locus_tag="ACHUN9_004241"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0697470.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          654 a          575 c          514 g          615 t
ORIGIN      
        1 gtataatttc agcagttgtt atttagaatg attaactgga ccgcaactga agcaagaata
       61 tcaggatgaa gcttaccgcc tcatcctgaa tggtcatttt accagtgcgt taatgacggt
      121 ttccatcgcc tgcttttcca gctcactgcg ctgaatgcgt ttattggcta atgcagtacg
      181 taatacgcct gcgataacaa tatgctttgg ttcctgcccc agatcgcgca tttccagcac
      241 aactttaccg acaactctgc acatttctcg gtacagttca tcctcttttg tccgatttcc
      301 cattctttat acctatttat catgccaact atcagcatat atcaatctaa ccaattaaac
      361 aaaaatcaaa caaaatcaga caaataacgc gataaattat tttaactgta gcaattgagc
      421 gatgatatat ttatacaccg gatgaacttt cacttatcct cacactgaca acttcggcac
      481 cagacgttgc gcaaacagcg aagtttttgc gtaacctttt ccctggaacg ttaaatcttt
      541 gataacaatt tattgtctaa caagttgtat attttttgaa acgctgtttt tgttttcctt
      601 ttggattaat ttcagcgtat aatgcgcgcc aattgactct tgaatggttt cagcactttg
      661 gactgtagaa ctcaacgact caaaaacagg cactcacgtt gggctgagac acaagcacac
      721 attcctctgc acgctttttc gatgtcacct atccttagag caaggcacca ccactttcgt
      781 aataccggat tcgctttccg gcagtgcgcc cagaaagcaa gtttctccca tccttctcaa
      841 cttaaagact aagactgtca tgaaaaagac caaaattgtt tgcaccatcg gaccgaaaac
      901 cgaatctgaa gagatgttag ctaaaatgct ggacgctggc atgaacgtta tgcgtctgaa
      961 cttctctcat ggtgactatg cagaacacgg tcagcgcatt cagaatctgc gcaacgtgat
     1021 gagcaaaact ggtaaaaccg ccgctatcct gcttgatacc aaaggtccgg aaatccgcac
     1081 catgaaactg gaaggcggta acgacgtttc tctgaaagcg ggtcagacct ttactttcac
     1141 cactgataaa tctgttatcg gcaacagcga aatggttgcg gtaacgtatg aaggtttcac
     1201 tactgacctg tctgttggca acaccgtact ggttgacgat ggtctgatcg gtatggaagt
     1261 tactgccatt gaaggtaaca aagttatctg taaagtgctg aacaacggcg acctgggcga
     1321 aaacaaaggt gtgaacctgc ctggcgtttc cattgccctg ccagcactgg ctgaaaaaga
     1381 caaacaggac ctgatctttg gttgcgaaca aggcgtagac tttgttgctg cttcctttat
     1441 tcgtaagcgt tctgacgtta tcgaaatccg tgagcacctg aaagcgcacg gcggcgaaaa
     1501 catccacatc atctccaaaa tcgaaaacca ggaaggcctc aacaacttcg acgaaatcct
     1561 cgaagcctct gacggcatca tggttgcgcg tggcgacctg ggtgtagaaa ttccggtaga
     1621 agaagttatc ttcgcccaga agatgatgat cgaaaaatgt atccgtgcac gtaaagtcgt
     1681 tatcactgcg acccagatgc tggattccat gatcaaaaac ccacgcccga ctcgcgcaga
     1741 agccggtgac gttgcaaacg ccatcctcga cggtactgac gcagtgatgc tgtctggtga
     1801 atccgcaaaa ggtaaatacc cgctggaagc ggtttctatc atggcgacca tctgcgaacg
     1861 taccgaccgc gtgatgaaca gccgtctcga gttcaacaat gacaaccgta aactgcgcat
     1921 taccgaagcg gtatgccgtg gtgccgttga aactgctgaa aaactggatg ctccgctgat
     1981 cgtggttgct acccagggcg gtaaatctgc tcgcgcagta cgtaaatact tcccggatgc
     2041 caccatactg gcactgacca ccaacgaaaa aacggctcat cagttggtac tgagcaaagg
     2101 cgttgtgccg cagctggtta aagagatcac ttctactgat gatttctacc gtctgggtaa
     2161 agaactggct ctgcagagcg gtctggcaca caaaggtgac gtcgtagtta tggtttctgg
     2221 tgcactggta ccgagcggca ctactaacac cgcatctgtt cacgtcctgt aatattgctt
     2281 ttgtgaatta atttgtatat cgaagcgccc tgatgggcgc ttttttattt aatcgataac
     2341 cagaagcaat aaaaaatc
//
LOCUS       DAZOYS010000337         2354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOYS010000337 DAZOYS010000000
VERSION     DAZOYS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.340"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..785
                     /gene="nirC"
                     /locus_tag="ACHUN9_004242"
     CDS             <1..785
                     /gene="nirC"
                     /locus_tag="ACHUN9_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0697471.1"
                     /translation="CAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL
                     DPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMWAILPQTWL
                     GNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAI
                     WMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNL
                     LWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            804..2177
                     /gene="cysG"
                     /locus_tag="ACHUN9_004243"
     CDS             804..2177
                     /gene="cysG"
                     /locus_tag="ACHUN9_004243"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0697472.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          514 a          597 c          658 g          585 t
ORIGIN      
        1 agtgtgcggc taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt
       61 gggtcagctc cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc
      121 tcggtaattt gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg
      181 ccttaacgct ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca
      241 cctttggggt aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa
      301 cctggctggg taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg
      361 gtagcctgct gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg
      421 caccggcaat ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta
      481 tctggatggc gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc
      541 tggcatttat cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct
      601 cctggttcgg caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt
      661 gggtgacgct gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg
      721 ctacgccgaa agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg
      781 gttaattact aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc
      841 gcgactgtct gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag
      901 acgcaggcgc tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg
      961 cagatgcagg catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct
     1021 gctggctggc gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg
     1081 ctgaagctcg tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta
     1141 tgccgtcgat tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc
     1201 cggttctggc gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc
     1261 aggtggcgaa atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga
     1321 gtgagcgtcg ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg
     1381 caaacaacga tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg
     1441 accatcgcgg tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc
     1501 tgaaaggact gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg
     1561 acgatattat gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg
     1621 gataccactg tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag
     1681 gcaaacgcgt ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag
     1741 agctggaaac actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag
     1801 cttctggttg ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg
     1861 tacgcttaat taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg
     1921 cagaaaaaca gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa
     1981 agctgattga acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg
     2041 cagtcacgca gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa
     2101 tgaacagtcc atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact
     2161 ggttctccaa ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt
     2221 ttatatattc agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata
     2281 ttcaccgtta cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt
     2341 cctgtatcgt tctt
//
LOCUS       DAZOYS010000338         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOYS010000338 DAZOYS010000000
VERSION     DAZOYS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.341"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHUN9_004244"
     CDS             20..238
                     /locus_tag="ACHUN9_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0697473.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHUN9_004245"
     CDS             240..605
                     /locus_tag="ACHUN9_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0697474.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHUN9_004246"
     CDS             602..1267
                     /locus_tag="ACHUN9_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697475.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHUN9_004247"
     CDS             1267..1632
                     /locus_tag="ACHUN9_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0697476.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHUN9_004248"
     CDS             2087..2242
                     /locus_tag="ACHUN9_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0697477.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          580 t
ORIGIN      
        1 ctcgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ctatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggttt t
//
LOCUS       DAZOYS010000339         2329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOYS010000339 DAZOYS010000000
VERSION     DAZOYS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.342"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>2329)
                     /locus_tag="ACHUN9_004249"
     CDS             complement(<1..>2329)
                     /locus_tag="ACHUN9_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0697478.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYS
                     EQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYE
                     LATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHT
                     TAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEK
                     TDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVW
                     RRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENG
                     EQAKARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAE
                     QMAAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHL
                     QQSLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFID
                     SLGLKFHVNGDPSDFNQAVEYLKQDSQMKETIDFLS"
BASE COUNT          371 a          792 c          624 g          542 t
ORIGIN      
        1 ttgataaaaa atctatagtt tctttcattt gtgaatcttg ttttaaatac tcaacagcct
       61 gattaaaatc acttggatca ccgttaacat ggaattttaa acccaaagaa tctataaacc
      121 gaacagggtt taatggatat ttatacagat tccattcacc tctcagccct atcggatcct
      181 gggtgatata ccttccctgc aacgggtcat agtaccggtt gcggttgtag tacagccccg
      241 actcctcatc atactgctgc cccggcagac ggagtgactg ctggaggtgc tgcgcgctgg
      301 tttcgcccag caggtttccc cattcgtcat actccccctg ccacgccgtt tccccttccg
      361 ggctgatgag cgccagcggc agtccccggt ggtcgcagtg gtaaagatga agtttcctct
      421 ccgggatgta ttccgcctcc agctgcgcgg ccatctgctc cgccgtcagc ccgcactgcg
      481 caagccatgc ctcactctcc gcactcaccg ctcccgcgcg cagttcccgc tccagcctcc
      541 ccagcatcac cgacagctcc gccggtagcg tcacacccgt gtcctcctgc aacacctccg
      601 ccaggctccg gtgccgcgcc ttcgcctgtt cgccattctc tgtttcgatt ctcaggagcg
      661 gcgtgaagct ccccggctgg tataccgtct ggatacgcgt ggtgccggtc tgtatcgtcg
      721 tcagcctgtc accgtcccac ccgtaccatg tcacctccgg tttacgcgac agcgacatcc
      781 accccgtcag gtcacgctcc cgccgccata cccgttttcc cgtccggcgg cccagcgggt
      841 catagaggta ccggctctcc acctgtggtt cgccgtgctg tatccgcgtg tagaacacca
      901 ggcggtgctg gctgtcgtag tgatagtggt gggtgcgctc gtcgtgcatc cggataaccc
      961 cttccgggat acggtccgtc ttctccgcca gcctgccgta ttcatcgtag cgatagacat
     1021 agtgcgcatc ttccgcgatg cggttatccg gccacgccgt gagggtgctg tccgggtgca
     1081 gttccgggtc cggcagccga tttcctgccg ggtctgttgc atacgggata tcgatatcca
     1141 ggttcgctgc cgtggtgtgg acgcccgtca gcctccccgt gtcgctgtaa cggtactccc
     1201 ggctctcctg cgggccactg atgcggatta gctgtccgtt gtcgttccag tcgtagtcac
     1261 ggtcaagctg cggaaggttc aggtgcctgc tccggagctg gccgctggta ttccaggcgg
     1321 tggcaagttc atatgcctcc ccgccgaagc tgcgggccgt ctcacggtgc agccggtccc
     1381 gcgtgtactc gaccagcggt gttccgccca gcttcatccc cgcaagataa ccgctgccat
     1441 acgtcagcca ctccaccggc ggcaggccgt ccggctcctg acgggtcgcc agcccctgtt
     1501 cgctgtacgc gtgccccgtc tcatgctccc acagcatctc ccccgtctcc ggggtctcca
     1561 ccgtctgccg ttcgcccgtc agacggcctt tatcgtcata gccgtagtgg accgccacac
     1621 ggtggccttc actcaggtgg ctgatttctg tcagccagcc gtggtcgtca tactgccact
     1681 gctccgccgg ttcaccgttc accgtgcgcc gggtgatacg gtccgacgca tcgtaatgcc
     1741 agagggtgat aagcccctca tcttcactct gcgtgagttt tccggtcagg tcatactggt
     1801 aacgttgcgt ccggccgtca aagccgcgct gctccgtcag cctgtcgacc gggtcatacc
     1861 ggaacgtgga ctggctgccg ttctcgttgg tcagcacagt gatgcgcccg gcggcgtcat
     1921 accccatgct tcgcgtcaga ccgccctgcg tggtgctgac ggcctttccc cacgcatcat
     1981 actgtatctc actgcggctg ccgtccgggg caacgatagc ggtgaggtct cctgcggcgc
     2041 tgtactcata acgggtttca cgcccctgcg catccctctg actgaccagt tgaccgcgcg
     2101 ggttataact gctgtaagtg ctgatgcctt cttcccggtg aacggcgatt tgttgaccgt
     2161 accggtcata ctcataccgc gtcgtgtacc ccgagcagtc cgtaaacgcc tgaagctgac
     2221 cgtaccggtt tcgcgttatt ctccgcctca ccccggttcc gtcttccacc gcacagggca
     2281 ggccactgcg ggaagaatca taaaaccagc gcgtaatgtt accgttgcg
//
LOCUS       DAZOYS010000340         2293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOYS010000340 DAZOYS010000000
VERSION     DAZOYS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.343"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            443..898
                     /locus_tag="ACHUN9_004250"
     CDS             443..898
                     /locus_tag="ACHUN9_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0697479.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            898..1068
                     /locus_tag="ACHUN9_004251"
     CDS             898..1068
                     /locus_tag="ACHUN9_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0697480.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1061..1351
                     /locus_tag="ACHUN9_004252"
     CDS             1061..1351
                     /locus_tag="ACHUN9_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0697481.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1348..1710
                     /gene="rusA"
                     /locus_tag="ACHUN9_004253"
     CDS             1348..1710
                     /gene="rusA"
                     /locus_tag="ACHUN9_004253"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0697482.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1707..1847
                     /locus_tag="ACHUN9_004254"
     CDS             1707..1847
                     /locus_tag="ACHUN9_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0697483.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1933..>2293
                     /gene="quuD"
                     /locus_tag="ACHUN9_004255"
     CDS             1933..>2293
                     /gene="quuD"
                     /locus_tag="ACHUN9_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0697484.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMD"
BASE COUNT          596 a          520 c          585 g          592 t
ORIGIN      
        1 aattaccagc aatagatgat tttctttttc cacttctatt caaagtggct ttaattacac
       61 caccaccatc cttagagtta aataattgaa tgtccactcc agcagttggt tgtatatatt
      121 ttgaaagctc tttcttgaac ttgttaatat atttatcttt gttttctttg ttggtcattg
      181 gttagatcca tagttctggc tgtgattagg tgaaagtata caaggttttt aaagcgaaga
      241 tcactgtttt ggcttcaaga tagttttgat ttatcgtaat caatccgcca taatcctctc
      301 atcggagcct gaacaactcc ggtgacttct gcgcatttaa ggggacttaa atgcgaccac
      361 aatctgaact catcaccttg tcacagatgc agaaatgcac ctgcgatttt ctgcattctg
      421 cggtttccgt tagggaggcc gtatgactct gccagtagac ggcatcaaac tccatcgcgg
      481 taacttcgcg gccatcggcc agcagattca gccattgctg gatgccgggc aatgcttacg
      541 cctgcaggtt aagccgtggc gcgagaagcg cagcctgtcg cagaacgcgc tcagccacat
      601 gtggtacacg gagatcagcg aatatctcat cgcccgcggc aagaccttcg ctacgcctga
      661 gtgggtcaaa gacgcgatga agcacaccta tctcggctac gaaagcaaag accgtgtaga
      721 tgtcgtgtcc ggcgaggtca ccaccgtcca atccctccgc catacgtcag agctggaaac
      781 cggcgagatg tacatcttcc tgtgcaaagt cgaagcctgg gcgatgaata tcggctgcca
      841 cctgacaatt ccgcagagct gtgaatacca gcagctgcgc gataagcagg aggcgtaatg
      901 gctacaccgc ttattcgtgt catgaacggg cacatctaca gagtatcaaa tcgtcgtaag
      961 cgtaagcctg agctgaagcc atccgaaata ccaacactgc tcggatatac cgctagcctg
     1021 gttgataaaa aatggttgcg actggcagca aggaggaatc atggctgatt tgagaaaagc
     1081 agcgcgtggt cgggaatgcc aggtaagaat ccctggcgta tgtaatggca atcctgaaac
     1141 gtcagtactg gcacatatcc ggctggctgg attgtgcggt accggtatca aaccgccaga
     1201 cctgattgcc accattgcat gttctgcctg tcacgacgaa atcgaccgcc gcacatattt
     1261 tgtcgatgct gagtatgcaa aagaatgcgc gctggaaggt atggcgagaa cgcaggttat
     1321 ctggctgaaa gagggggtta ttaaggcgtg aatacctaca gcatcacatt accctggcct
     1381 ccgagcaata accgctatta ccgccataat cgcgggcgca cacacatcag cgcagaaggg
     1441 caggcatacc gcgataacgt cacccgaatc attaaaaacg caatgctgga tatcggcctg
     1501 gctatgccag tgaaaatccg tattgagtgc cacatgccgg atcgccgtcg ccgtgacctg
     1561 gataatctgc aaaaagccgc ttttgacgca ctcaccaaag caggtttctg gctggatgat
     1621 gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta ccaaaggtgg gaggctggaa
     1681 ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt ttatatggca gaacttcttc
     1741 gccaccgctg ggggcatctg cgcttatatc gtttccccgg ttctgttttg accgattacc
     1801 gaatactgaa gaattacgcc aaaaccctga caggagcagg agtatgaagt cagagataac
     1861 aatcaactaa tactgttttg ttgatttttg cttgtaattg gcgttctggt ctgatttttg
     1921 tggagtaagt tgatgcgtga tattcagatg gttcttgagc gttggggagc gtgggcggct
     1981 aataatcatg aagatgtgac ctggtcgtcc attgccgccg gttttaaggg attaattact
     2041 tcaaaagtaa aatctcgccc gcaatgttgt gacgatgacg cgatgattat ttgcgggtgc
     2101 atggcccgtc tgaaaaagaa caacagcgat ttgcacgatt tattagtaga ttattatgta
     2161 gtcggtatga cattcatgtc actggcaggt aagcattgct gctctgatgg ttatatcggg
     2221 aaaaggttac agaaggctga gggcataatt gaagggatgt taatggcatt agatatccgg
     2281 ttagagatgg ata
//
LOCUS       DAZOYS010000341         2276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOYS010000341 DAZOYS010000000
VERSION     DAZOYS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.344"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..677
                     /locus_tag="ACHUN9_004256"
     CDS             <1..677
                     /locus_tag="ACHUN9_004256"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0697485.1"
                     /translation="PLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVGR
                     AVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLACRRRGL
                     IVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNV
                     DLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFM
                     TARGYH"
     gene            complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHUN9_004257"
     CDS             complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHUN9_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0697486.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          599 a          590 c          559 g          528 t
ORIGIN      
        1 ctccattaat tgccgttatt ggtagcgatg gttcaggcaa gtcaacggtg tgtgaacatc
       61 ttattaccgt tgtcgaaaaa tatggtgctg ccgaaagagt tcatttagga aaacaggccg
      121 gaaatgtcgg tcgtgcagtg acaaaattac cgttgatggg aaaatcctta cataaaacaa
      181 ttgaacgaaa tcaggtgaaa acagcaaaaa aattgcctag accagttccg gcgctggtaa
      241 ttacagcgtt tgtcgcccgt cgcttactgc gctttcgtca tatgcttgcc tgtcgtcgtc
      301 gcgggttaat tgttctaacc gaccgttatc ctcaggacca aattcctggc gcttacgatg
      361 gtacggtgtt cccacctaac gttgaaggtg gtcgttttgt ctcatggctg gcaagccagg
      421 aacgtaaagc gtttcactgg atggcgagcc ataagcctga tctggtcatc aaactcaatg
      481 ttgaccttga agttgcctgt gcacgtaaac ccgaccataa acgggaatcg ctggcgagga
      541 agattgccat aacgccacag ttaacctttg gtggtgcaca actggttgat atcgatgcca
      601 atcagccact ggaacaggtg ttggttgatg cagaaaaagc gattacggat tttatgaccg
      661 cgcgtggtta tcactagtca aaaatggaaa tgcccgatcg ccaggaccgg gcattttcag
      721 gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc
      781 ggtaaagtaa acacccacgc catcaggatg ctggttaccg ttttacgctg taacccaccg
      841 ccgtccacca ccatcgtccc tgctaccgca gacgagagga cgtgtgttgt ggagacaggc
      901 atcccaatat aactggcaag gccgatagac actgccgccg tcatttgtgc cgccatgcct
      961 tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc
     1021 cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacacagga
     1081 gcgtactcaa tggtgcttaa catatcgctg cgcagttttt tcagcaggtt ctggtcttct
     1141 ttactgacgc ctggcagttt cgccagcttc gcggaggtat cagagatgca cagcataatg
     1201 cggcgcagct ggctgcgctg actcacgctt aacggctcgt aactttccat attgcctggc
     1261 agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactct
     1321 gttacttgcg tgccatcagt cgatgctgca ggcaatggag gttccatcgc aatcaacttc
     1381 tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtgacggc atcgcgggta
     1441 cgggtaattt catagccgga cgcgttcata ttgacgacga agccagcagg ggcaatcccc
     1501 accagtacca gcatcaccag gccgatccct ttttgtccgt cgttcgcgcc gtgcgaaaac
     1561 gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg tttacgtttg
     1621 ccttttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtcccg
     1681 ctccagtagc gtcgcagcag gaatatcagg cctcccgcaa tgaccaggcc gacgataggg
     1741 gaaacaatca gcgaggagaa aattttggtc acttcacgca ggtttaacgc atccatcacc
     1801 gatgatccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcacc aatcaaggtg
     1861 tgcgaactgg aggccggtaa accgaagaac cacgttccca ggttccagat aatcgccgcc
     1921 agcagcatgg aaaagaccat cgccaggccg tgggttgacc ccatattcag caacaaatcg
     1981 gttggcaaca tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa
     2041 aagttaaaaa atgccgccat caccacagca agttgtggtt gcatggcacg agtataaata
     2101 acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca
     2161 aatgccagag caagcaataa caaaagccct gtgtatatat caaggccaac aaataaattt
     2221 agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca gtgact
//
LOCUS       DAZOYS010000342         2266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOYS010000342 DAZOYS010000000
VERSION     DAZOYS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.345"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(42..260)
                     /gene="ygdR"
                     /locus_tag="ACHUN9_004258"
     CDS             complement(42..260)
                     /gene="ygdR"
                     /locus_tag="ACHUN9_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0697487.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            complement(398..1111)
                     /locus_tag="ACHUN9_004259"
     CDS             complement(398..1111)
                     /locus_tag="ACHUN9_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0697488.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1180..1869)
                     /gene="mutH"
                     /locus_tag="ACHUN9_004260"
     CDS             complement(1180..1869)
                     /gene="mutH"
                     /locus_tag="ACHUN9_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0697489.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2055..2201
                     /gene="ygdT"
                     /locus_tag="ACHUN9_004261"
     CDS             2055..2201
                     /gene="ygdT"
                     /locus_tag="ACHUN9_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0697490.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
BASE COUNT          609 a          577 c          522 g          558 t
ORIGIN      
        1 cttaactgat cggcattaca gccggatgct gaccttattt gttaacgttc aataatttgc
       61 gaaacatcat cacggttaat ttgcatcgcg ttaccttgct gatcgtgata actcaccagc
      121 ccggtatcat catcaatttc aggttttcca tcggtcaaaa tcatacggcc atctttggtc
      181 gccatgacgt aatcactgga acacccggaa acagcaaacg ccagtccgac tgcagaaatt
      241 attactgccc attttttcat ccggttatcc tcatttggcc cacctatgat aatagtctgt
      301 ttataaaccg acgttatata tcttgaaagc aggaaaatct taaactgtga gggtaacagg
      361 agtgagtacg ccctcccgca ggagagcgta cggagtatca gagcgggttc tttttgttgc
      421 gaatcaggtt gaggctttca accgcaatag agaagaacat cgcgaagtag atgtaacctt
      481 tcggtacgtg gatgtcgaaa ctttccagaa tcagggtaaa gccgaccaga atcaggaaag
      541 agagcgccag catttttacc gaaggatggc gttcgacaaa atcaccaatc gagcgcgcgg
      601 cgaacatcat cacgcctacg gcaatcacca cggcggccat cataataaac aggtgatctg
      661 acagacccac agcggtaatc accgagtcga ggctgaagat aatatccagc agcataatct
      721 gcacgatagc gccaaggaat gatgaaacgc gtgttttcag cccttcttct tcaccttcaa
      781 tggattcgtg gatttccttg ctggctttcc agataaggaa caagccaccc agaagcagaa
      841 tcaaatcacg ggcggaaatt tcctgactga atattgtaaa aagcggattt gtcaggcgcg
      901 taacccaggc gattgatgcc agcagcgcca gacgcataac catggctccc gccaacccca
      961 gacggcgcgc atgagcacgt tgtgctgtgg ggagctttgc caccaccaga gaaaggaaaa
     1021 taatattgtc gatcccaaga acgatctcca gcagcgtcag cgtaccgagc gcaagccagg
     1081 cgttaggatc ggttatccat gcaaataaca tctaaaaagc cctgccaaaa aaatgaagcg
     1141 gtaattatat gcccggaaag cggcaggtca aagcgatggc tactggatca gaaaatgacg
     1201 ggccagtagt gcactggtga aattcttctt caaataaaag ccgcgtggca gcgtcagaat
     1261 ccgttcgccc cgggcaccaa tggcttcggt aagcgctttc gcattcgctg ctttcggtcg
     1321 tatctgtaaa tactccccgt gacgagcggt gatccgctca acctgaccga gaacaatcat
     1381 atccattaat tcttcccagt cttcgcgtag ctgtcggtct tcctcttcat tcgggctcca
     1441 cagcaacggt gatcctacgc gacgctgcgc cagcgggatg ctgcgctcac cttcaaccgg
     1501 tatccacagt acgcgtttga gcttgtggcg cacatggctg gtttcccagg tcaccccgct
     1561 attacccgtt aacggggcaa cacaaacgaa tgttgtttcc agcggacgac caagactatc
     1621 cacagggata gttttaagtt ccacgcccag agcagcaaaa tcttgctcag gtttactccc
     1681 tgcgctggca cctagccaga tctccagtaa cacgccaatc cagcccttat cgcgttttaa
     1741 attctctggc gtaaccagcc cggcaagtgc cgccagttct cccaatgtat aaccagaaag
     1801 ttgctgtgct tgcgctaaca actgttcttc agtttcggga ggagagagga gtgggcgagg
     1861 ttgggacatg tcatgatacc ttggttaaaa aacaaacagc aattatgtgc cgaatattcg
     1921 cagtgtaact ttttccacat gtgttgttca attctctgat ttatattgct ttttaggctg
     1981 ccggtaatcg agttgcgcaa cactgcaata ggttttccaa atctggtcac tgaaaataaa
     2041 caggatctta caccatgtta tccacagaaa gctgggataa ctgtgaaaaa cctccactac
     2101 tgtttccatt tacagccttg acgtgcgacg aaactccagt ttttagtggt tcagtgctta
     2161 acttagtggc acattctgtg gataaatacg gcattggttg atctttcgcc aacccgtaaa
     2221 tgagaggtgt gacctgggtc acgaattact ctttttcatt actcaa
//
LOCUS       DAZOYS010000343         2266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOYS010000343 DAZOYS010000000
VERSION     DAZOYS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.346"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(128..1132)
                     /gene="argF"
                     /locus_tag="ACHUN9_004262"
     CDS             complement(128..1132)
                     /gene="argF"
                     /locus_tag="ACHUN9_004262"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0697491.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1294..1710
                     /gene="rraB"
                     /locus_tag="ACHUN9_004263"
     CDS             1294..1710
                     /gene="rraB"
                     /locus_tag="ACHUN9_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0697492.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1756..2259)
                     /locus_tag="ACHUN9_004264"
     CDS             complement(1756..2259)
                     /locus_tag="ACHUN9_004264"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0697493.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          577 a          559 c          551 g          579 t
ORIGIN      
        1 taaatcctcc ggaagattta cgtatagcaa taaaaaaatt ggggtggaat aattgaacta
       61 tcactgcata aaataatgtg acgccgggat gtttttgtat ttcccggcat ctttatagcg
      121 atatcaatta tttactgagc gtcgcgacca tcaccgcttt gatggtatgc atgcggtttt
      181 ccgcctgatc aaatacaatg ctggcggcag attcgaagac ctcatcagtc acttccattc
      241 cgccatgtag gccaaactct tccgccattt ttttgccaag cgtcgtttgg tcgtcatgaa
      301 acgcgggcag gcagtggagg aatttgacct ccgggttacc ggtcaactgc atcatcttgc
      361 tgttcacttg ataatcacgc agcaatgcaa tgcgttcagc ccatttctct tttgcttcac
      421 ccatcgacac ccatacatcg gtatagataa agtcagcacc ttcaactcct ttagctacat
      481 cttccgtcag cgtaatattc ccgccatttt gttgtgccag ggcgcggcat tccgtaacca
      541 gcgcagcttc cggccagcat gcctgtggcg cgaccagacg caaatccaga ccggtaagcg
      601 ctgcagcttc gagcatcgaa ttgcccatgt tgttacgtgc gtcacctgca tagactagcg
      661 tcatttcgtt gaacgttttg ccgggcaaat gctcctgcat ggtgaggaga tccgccagca
      721 gctgcgtggg gtgaaactca ttggtcaggc cgttccatac cggcacgcca gcgtattccg
      781 ccagtgtttc gacaatctcc tgaccatagc cgcgatactg aataccgtca tacatgcgac
      841 caagcacgcg ggcagtgtct ttaatcgact ctttatgacc aatttggctg ccgcttgggc
      901 cgagataagt aacacgagcc ccctggtcat atgcggcaac ttcgaaagag catcgggtac
      961 gggtcgagtc tttttcgaag atgagcgcga tgtttttacc agtgagtttg gcttcttctt
     1021 taccgctttt cttatcggct ttcagcttcg cggctaactg cagcaggctg ttgagttcag
     1081 ctggcgtgaa atcgagtaat ttcaggaaat gcttatgata aaacccggac ataggtccct
     1141 cctgtggcta acgccttaat gaattaaaat tcaatttata tggatgatta ttcatttgca
     1201 agtctaaagc ataaatcttt atcacaaagg tggaggcaat gtcagtggtg tgtgacaata
     1261 agagtatcgg caggacatta agaggaatga gccatggcaa acccggaaca actggaagaa
     1321 cagcgtgaag aaacacgttt gattattgaa gaattactgg aagatggcag cgatccggac
     1381 gcgttgtaca ccatcgaaca ccatctttcc gcagacgatc tggaaactct ggaaaaagcg
     1441 gcagttgaag cgtttaaact cggttacgaa gtgaccgatc cagaagagct ggaagttgaa
     1501 gatggtgata tcgtgatttg ctgcgacatc ctcagcgagt gcgcgttgaa tgccgatctg
     1561 atcgatgccc aggttgaaca actgatgacg ctggcggaga aatttgacgt tgagtacgac
     1621 ggttggggca cttactttga agatcctaac ggcgaagatg gcgacgatga agattttgtc
     1681 gatgaagacg atgacggggt tcgccactaa ttaattgatt aaaatgcccg atagcacata
     1741 ctagcgggca tttttttaga gttcgcgtag catccgcact tcacaatcga catggcccgt
     1801 gcagccaagc gcatagtcga tatgctcaaa gcccaaatgc tcataaagcg caatggcttc
     1861 ctttaaaaaa gcggtcgttt ccagatagca gcgtttgaag cccatctctc gtgcctgctc
     1921 catcgccatt aaggccagtt ttttcgctag ccctttaccg cgaatagccg gaagaaaata
     1981 catcttttgc agttcgcaaa tgtccgactc actcccggtt aatggcgcaa tcccaccgcc
     2041 gccgaccact tcaccctcgt actcaaccac ccaatatgca tggccaggct gactatacac
     2101 ttgatacagc tcgtcaagat tcggatcggc gacggtgtag cctttatcag cggtaagacc
     2161 gtattcggcg gatacctgac gaatgacgcg ggcgatagca ggattatcca gcagcgtcag
     2221 gcgacgcatt acaggtgatt gcggcgcaat gttattcata gtttaa
//
LOCUS       DAZOYS010000344         2251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOYS010000344 DAZOYS010000000
VERSION     DAZOYS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.347"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..221
                     /locus_tag="ACHUN9_004265"
     CDS             <1..221
                     /locus_tag="ACHUN9_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR0697494.1"
                     /translation="VRVVISEFVADGTILMGDFSNVILANWEGQEVDYDDTSYRSSNT
                     IVYRVWDYSDIALAHSEAFVNIVIGKAE"
     gene            235..558
                     /locus_tag="ACHUN9_004266"
     CDS             235..558
                     /locus_tag="ACHUN9_004266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697495.1"
                     /translation="MRVFKDTQCDAFLNAFGQSIQTCTGSTFTGIVEVLPVSIEAAGG
                     FIESVETYLTIRKDDLITANVAIGTVLIIDGVNQTIYNIEDDLSGMVNCYFRTSAGAS
                     FAEDY"
     gene            560..952
                     /locus_tag="ACHUN9_004267"
     CDS             560..952
                     /locus_tag="ACHUN9_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697496.1"
                     /translation="MMLVQKVRNTMKQLINATKNLTVSRDVDVFEQIAFDYSLTSLSF
                     SNERRAGNFAIQYLISPKPESGNTAPSITYDQIISTFDKEKTSAFSDAGLIMLSCSYE
                     QSDIVTDPITGSVSLSFTINIQVTEKTR"
     gene            970..>2251
                     /locus_tag="ACHUN9_004268"
     CDS             970..>2251
                     /locus_tag="ACHUN9_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR0697497.1"
                     /translation="MSDIFSGRELSVQYNADIGNRTPQGAGNVVIKQINTFPTLTINS
                     EANNFETYDSDYRTVLLSDKSVAPFDIVVNYLPDDPTHQFLDNAAKSQQLFQIIIQYQ
                     MNKDENTITYAMVNGYITSYKLDGDKGSVVTKAYSFAPHDVIARAMTINALSPIYQGD
                     YGLGSNTTDVPQYSPSVPTGNSFIKVPSSQAGNPAGADLMGVGLVDGTTVSSLAMTKT
                     GTLSIYAKNATTAWTRIYTATQMDGRYVPLTRTINGHALTANLVLNSEDTGSVPDTRT
                     VNGKPLSEDVVLTSTDTGSVPDTRTVNGHVLSEDIVLSSEDTGSVPDTRTVNGHVLSE
                     NVVLSSEDTGSVPDTRMVNGHVLSEDVVLTSTDVGLGKVTDDAQLTIAGNLSDLGDVE
                     EARINLAVYSMDEVDAEVTALADSITSLDSASMKK"
BASE COUNT          708 a          427 c          485 g          631 t
ORIGIN      
        1 aagtacgtgt agtgatttct gaattcgtag cagatggtac tattctaatg ggcgatttct
       61 ccaatgtaat tctcgctaat tgggaagggc aggaagtaga ttatgacgat acatcctatc
      121 gttcaagtaa cactatcgta tatcgcgtat gggattactc agatatcgca cttgcacact
      181 ctgaagcttt cgtaaatatc gttattggta aagcagaata ataaggatta gataatgaga
      241 gtatttaaag atacgcagtg tgatgcgttt ctaaatgctt tcggtcagtc tatccaaact
      301 tgtacgggaa gtacttttac aggaatagta gaagtacttc ccgtttctat tgaggcggca
      361 ggtggattta tcgaaagcgt ggaaacatac ctaacaatca gaaaggacga tctaataacc
      421 gcaaacgtgg caattggtac agtactaatc attgatggtg taaaccagac aatctataac
      481 attgaagatg atctatcagg tatggttaat tgctatttcc gtacttctgc tggtgcttca
      541 tttgcagagg actactaata tgatgttagt acaaaaagta cggaatacga tgaagcagct
      601 tatcaatgct actaagaatc tgacagtatc acgtgatgtt gatgtatttg aacaaattgc
      661 ttttgattac tcattaacga gtttaagttt cagcaatgaa cgccgggcag gtaactttgc
      721 aattcagtat ctgatttccc ctaaaccaga atcaggtaat acagccccat ccatcactta
      781 tgatcagatt attagtactt ttgataaaga gaaaacttca gcattcagtg atgcaggttt
      841 aataatgctt tcgtgttctt atgaacaatc cgatattgtc acagatccta taactggctc
      901 ggtttcccta tcctttacga taaatattca agttacggaa aagacaagat aactaaaagg
      961 atataaagga tgagtgatat tttctcagga agagaattat cagtacagta taatgctgat
     1021 attggtaaca gaacaccaca gggagcaggc aatgtagtaa ttaaacagat taatactttt
     1081 ccaaccttaa caataaattc agaagcaaat aattttgaaa cgtatgatag tgattacagg
     1141 acagtactac tatctgataa atcagtagca ccatttgata ttgtagttaa ctacctacct
     1201 gatgatccta cacatcaatt cttggataat gccgctaagt ctcagcagct tttccagatc
     1261 atcattcagt accagatgaa taaggatgaa aataccatta cttatgcgat ggtgaatggt
     1321 tatatcacct cttataagtt ggacggtgat aaaggttcag tagtaaccaa ggcatacagt
     1381 tttgccccac atgatgtgat cgcacgtgca atgaccatta acgcactatc gccaatctat
     1441 cagggggact acggtttagg ttcgaacact acggatgtac ctcagtactc accatcagta
     1501 cctacgggta acagcttcat caaagtacca tcatctcaag caggcaatcc tgccggggcg
     1561 gatctgatgg gagtagggct tgtagatggc actactgtaa gttctcttgc gatgacaaag
     1621 acgggtactc taagtatcta tgcaaagaac gctacaaccg cctggacacg catatatacc
     1681 gctacacaga tggacgggcg atacgtaccc cttacacgta ccatcaacgg ccatgcactc
     1741 acggccaatt tagtactgaa cagtgaagat acaggttcag tacctgatac acgtactgtg
     1801 aatggtaagc ctctaagtga ggatgtagta cttaccagta ctgataccgg ctctgtacca
     1861 gatacacgga cagttaatgg tcatgtactc agtgaagata tagtactgag tagtgaagat
     1921 acaggttcag tacctgacac gcgtacagtg aacggtcatg tacttagtga aaatgtagta
     1981 ctgagtagtg aagatacagg ttcagtacct gatacacgta tggtcaacgg tcatgtactc
     2041 agtgaggatg tagtacttac cagtactgat gttggcttag ggaaggttac agatgatgcc
     2101 cagcttacga tagcaggtaa tctaagtgat ctgggggacg tggaagaagc acgtattaac
     2161 cttgctgttt actcaatgga tgaagtagat gcagaggtaa cggctttagc tgacagtatt
     2221 acttcccttg attccgcttc catgaagaaa g
//
LOCUS       DAZOYS010000345         2223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOYS010000345 DAZOYS010000000
VERSION     DAZOYS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.348"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..351
                     /locus_tag="ACHUN9_004269"
     CDS             <1..351
                     /locus_tag="ACHUN9_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0697498.1"
                     /translation="RWYLCPQVRGADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIV
                     ELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWL
                     NTTGADQQSPEHRS"
     gene            591..851
                     /locus_tag="ACHUN9_004270"
     CDS             591..851
                     /locus_tag="ACHUN9_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0697499.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1075..1149
                     /gene="tap"
                     /locus_tag="ACHUN9_004271"
     CDS             1075..1149
                     /gene="tap"
                     /locus_tag="ACHUN9_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0697500.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1142..1999
                     /gene="repA"
                     /locus_tag="ACHUN9_004272"
     CDS             1142..1999
                     /gene="repA"
                     /locus_tag="ACHUN9_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0697501.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          556 a          562 c          596 g          509 t
ORIGIN      
        1 cggtggtatc tgtgtccgca ggtacggggc gcggatatcg tccagctgaa cgggaatatc
       61 cggccacgat cacgccagtg gtggcagtta ttcaggatgg tgtctcagtg gcatgttgat
      121 gtggttatcg ttgagctgcg ttcgttcagt attgttgcag cagtagagct ggatgatgcc
      181 agccatttac gaccggaacg cagacgccgg gatattcttc tggaagaggt tctgaggcag
      241 gctggtattc cgttgctcag aagccacgat gccagaaaac tgctgcagat gaccggagaa
      301 tggctgaata caacaggggc tgatcagcag tcaccggaac atcgtagctg acgccttcgc
      361 gttgcttagt tgtccaaccc cggaaacggg aaaaagcaag ttttcctcgc tcccggcgtt
      421 tccataactg aaaaccatac tatttcacag tttaaatcac attaaacgac agtaatcccc
      481 gttgatttgt gcgccaacac agatcttcgc cacaattctc aagtcgctga tttcaaaaaa
      541 ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg aggaggcgag atgtcgcaga
      601 cagaaaatgc agtgacttcc tcattgagtc aaaagcggtt tgtgcgcaga ggtaagccta
      661 tgactgactc tgagaaacaa atggccgcag ttgcaagaaa acgtcttaca cacaaagaga
      721 taaaagtttt tgtcaaaaat cctctgaaag atctcatggt tgagtactgc gagagagagg
      781 ggataacaca ggctcagttc gttgagaaaa tcatcaaaga tgaactgcag agactggata
      841 tactaaagta aagactttac tttgtggcgt agcatgctag attactgatc gtttaaggaa
      901 ttttatggct ggccacgcca taaggtggca gggaactggt tctgatgtgg atgtacagga
      961 gccagaaaag caaaaacccc gataatcttc tttaactttg gcgagtcaga aagattaccg
     1021 gggcccactt aaaccgtata gccaacaatc aagctatgcg gggagtatag ttatatgccc
     1081 ggaaaagttc aagacttctt tctgtgctcg ctccttctgc gcattgtaag tgcaggatgg
     1141 tgtgactgat cttcaacaaa cttattaccg ccaggtaaag aacccgaatc cggtgttcac
     1201 accccgtgaa ggtgcccgga cgcttccgtt ctgcgggaaa ctgatggaaa aggcggtggg
     1261 cttcacctcc cgttttgatt tcgccattca tgtggcacat gcccgttcgc ttggtctgcg
     1321 tcggcgcatg ccaccggtac tacgtcgccg ggccattgat gcgctgctgc aggggctgtg
     1381 tttccactat gacccgctgg ccaaccgcgt tcagtgctcc atcaccacgc tggccattga
     1441 gtgcggactg gcgacggagt ctgatgccgg aacactttcc atcacccgtg ccacgcgtgc
     1501 cctgacgttc ctggcagagc tggggctgat tacctaccag acggaatatg acccgcttat
     1561 cggctgcaac attccgaccg acatcacgtt cacaccggcg ctgtttgccg ccctcgatgt
     1621 atcagaggag gcggtggcgt ccgcgcgccg cagtcgtgtg gaatgggaaa acagacagcg
     1681 caagaagcag ggactggata ccctgggtat ggatgaactg atggcgaaag cctggcgttt
     1741 tgtgcgtgag cgtttccgca gttaccagac agagcttaag tcccgtggaa tgaagcgtgc
     1801 ccgtgcgcgt cgtgatgcgg acaggcagcg tcaggatatt gtcaccctgg tgaaacggca
     1861 actgacgcgt gaaatctcgg aagggcgctt cactgccagt cgtgaggcgg taaaacgtga
     1921 agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt aaccgtaatt acagccggct
     1981 ggccacagct tccccctgaa agtgacctcc tcagaataat ccggcacgcg ccggaggcat
     2041 tcgcacgcct gaagcccgcc agtgcacaaa aaatcagcgc tacatacaaa aaacaacctc
     2101 atcatccacc ttctggtgca tccggtcccc cctgtttttg atacaaaaca cgcctcacag
     2161 acggggaatt ttgcttatcc acattaaact gcaagggact tccccataag gttacaaccg
     2221 ttc
//
LOCUS       DAZOYS010000346         2216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOYS010000346 DAZOYS010000000
VERSION     DAZOYS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.349"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            90..422
                     /gene="hdeA"
                     /locus_tag="ACHUN9_004273"
     CDS             90..422
                     /gene="hdeA"
                     /locus_tag="ACHUN9_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0697502.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            538..864
                     /gene="hdeB"
                     /locus_tag="ACHUN9_004274"
     CDS             538..864
                     /gene="hdeB"
                     /locus_tag="ACHUN9_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0697503.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            928..1575
                     /locus_tag="ACHUN9_004275"
     CDS             928..1575
                     /locus_tag="ACHUN9_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0697504.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1617..2147)
                     /locus_tag="ACHUN9_004276"
     CDS             complement(1617..2147)
                     /locus_tag="ACHUN9_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0697505.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVSLI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          663 a          427 c          498 g          628 t
ORIGIN      
        1 aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat tcgtgacggc
       61 tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt attcttggtg
      121 gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca gctgataaca
      181 aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa tccttccagc
      241 caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa gatgcggttt
      301 tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt actcaggata
      361 aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag aaagatatgt
      421 aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg aatatcttct
      481 ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg ttacaaaatg
      541 aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc tttgtcactg
      601 gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac ctgccaggaa
      661 tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct gcatgaagaa
      721 acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac tcaaattcct
      781 aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa
      841 gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca agttgagcca
      901 tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg tcttatactc
      961 gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg
     1021 ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt ttcgaaatat
     1081 ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg tattgctgct
     1141 caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat
     1201 attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgccatagg tatggttatt
     1261 ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgtt ggtgctggaa
     1321 gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca gctgacatta
     1381 gtgaacggta atatggtatc aatgctcgac tggtttaagc agcaaaaaat aaaaactgat
     1441 ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat ccagcttcat
     1501 gccacaacgt cgatagaaga cctgttgcga ttattaaaag gtatggcagg ggtgaaaggc
     1561 gtttcaatta gctaatctgt gtggcagcgt agccagactc accgtaagcc tgaaattcac
     1621 accagataat caatatgctg atggcgtacc agctcattga tccgcttaag attgaggatc
     1681 atcatgatat tgtgtttatg gcagtagaac gtttttagcg atattttcag tttctccaga
     1741 atatcttcag ttgtcatttg attcgcaatg cagctcagaa cctgatgttg cttgcgcgaa
     1801 agtgacacat tcgcttcagg cacagagcag taactgccat tgatgagtga gacaaaatca
     1861 atgatagtga tagtccgcgg aacaaaggtc accggtactt tccccagaca gcgtttaata
     1921 ttaaaggggg agaaaacaat gacatggaca ggttttaatc gttcaatttg agtgagaaaa
     1981 ttagaatgaa atacattatt cattaatgta tcaataatga cgaaggcatt ctgatgcaac
     2041 attacgtcga tctcttcttc gttctgtata tgaacgacat tacctttact cagaatgttt
     2101 ttcatcgcgg tgaagaacat cgtatccctg gtaattataa gaaacataac cgactcctgg
     2161 tgcggacatt aactttataa tcaatttgcc aaaggtaatt aacaggacag ggctta
//
LOCUS       DAZOYS010000347         2176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOYS010000347 DAZOYS010000000
VERSION     DAZOYS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.350"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            175..2160
                     /gene="yjcS"
                     /locus_tag="ACHUN9_004277"
     CDS             175..2160
                     /gene="yjcS"
                     /locus_tag="ACHUN9_004277"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0697506.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          582 a          572 c          528 g          494 t
ORIGIN      
        1 taataaattc gcgtaatttt ccctcttcag cacaaatgcc taataaaatg gatcgttcag
       61 ctcacacttc cacttattaa agtcgcaaat tcccaattat gataacccca gctttaaatg
      121 acgtattttg catggattac atatttacga cataaatgat aaatggagat ttacatgaat
      181 aactctcggt tattccgttt gagcagaatt gttattgcgt taactgccgc cagcggcatg
      241 atggtaaata ccgctaacgc gaaagaggaa gcgaaagccg ccactcaata tacccaacag
      301 gttaatcaga attacgccaa atcattaccg tttagcgatc gtcaggattt tgacgacgct
      361 cagcgtggat ttatcgctcc gctgctggat gaaggtattc tgcgcgatgc taatggcaaa
      421 ccatattatc gcggggaaga ttataaattt gatatcaatg cgcccgcgcc cgaaaccgtt
      481 aaccctagcc tgtggcgtca gtcgcagtta aacggtattt ccggcctgtt taaagtcacc
      541 gacagaatgt accaggttcg cggtcaggat atctccaaca tcacctttat cgaaggtgat
      601 accgggatta ttgtcatcga cccgctggtg acgccaccga gcgcaaaagc cgcccttgac
      661 ctttacttcc aaaatcgccc gcaaaaaccg attgttgcgg ttatttatac ccacagccat
      721 accgaccact atggcggcgt gaaaggcatt atctctgaag ctgatgtgaa atccggtaaa
      781 gtgcaggtta tcgcccctgc tggctttatg gacgaagcca tcagcgaaaa cgtactggcg
      841 ggtaatatta tgagccgccg tgcactttac tcctacggcc tgctgctggc gcataaccct
      901 cagggtaaca tcggcaatgg tcttggcgta acgctggcat cgggctaccc gagcatcatc
      961 gcaccgaaca aaaccatcac caaaaccggt gagaagatga ttatcgacgg cctggagttt
     1021 gacttcctga tgaccccagg tagcgaagca ccagccgaaa tgcacttcta tattccggcc
     1081 ctgaaagcgc tgtgtaccgc cgagaacgcc acgcataccc tgcacaactt ctacactctg
     1141 cgcggcgcga aaacccgcga caccagcaag tggaccgagt atctgaacga aacgctggat
     1201 atgtggggta acgacgcgga agtcctgttt atgccgcaca cctggccggt ctggggcaat
     1261 aagcatatca atgattatat tggtaaatat cgcgatacta tcaagtacat tcacgaccag
     1321 accctgcacc tggcgaacca gggctacacc atgaatgaaa tcggcgacat gattaaactg
     1381 ccgcctgcac ttgccaataa ctgggccagc cgtggctatt acggttctgt cagccacaac
     1441 gcccgcgcgg tgtataactt ctatcttggc tattacgacg gtaacccggc taacctgcat
     1501 ccgtatggtc aggtggagat gggtaaacgt tacgtgcagg cgctgggcgg ttctgcccgt
     1561 gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt accgctggtc ggcagaactg
     1621 ctgaaacagg tgattgccgc caacccgggt gaccaggtcg cgaagaatct gcaagcgaat
     1681 aactttgaac agctgggcta tcaggccgag tccgccacct ggcgcggttt ctacctgacc
     1741 ggcgcgaaag agctgcgcga aggggtgcat aagttcagcc acggcaccat cggttccccg
     1801 gacaccattc gcgggatgtc ggtcgaaatg ctgttcgact ttatgtccgt tcgcctcgat
     1861 agcgcgaaag ccgcgggtaa aaatatcagc ctgaacttca atatgagcaa tggcgataac
     1921 ctcaacctga cgctgaacga tagcgtgctt aactaccgta aaacactgca atcccaagct
     1981 gacgcctctt tctacatcag ccgtgaagat ctgcacgccg tgctgaccgg gcaagccaaa
     2041 atggcggatc tggtaaaagc gaagaaagcc aaaattattg gcaatggcgc gaaactggaa
     2101 gaaattatcg cctgtctgga taatttcgat ttgtgggtga atatcgtaac cccaaattaa
     2161 tgtcactcgc ttttgg
//
LOCUS       DAZOYS010000348         2156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOYS010000348 DAZOYS010000000
VERSION     DAZOYS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.351"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHUN9_004278"
     CDS             complement(34..954)
                     /locus_tag="ACHUN9_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0697507.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUN9_004279"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUN9_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0697508.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          537 a          480 c          502 g          637 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctat
//
LOCUS       DAZOYS010000349         2142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOYS010000349 DAZOYS010000000
VERSION     DAZOYS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.352"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..354
                     /locus_tag="ACHUN9_004280"
     CDS             <1..354
                     /locus_tag="ACHUN9_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0697509.1"
                     /translation="LDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQ
                     RNGGVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRP
                     LQKLINGTWYNVASI"
     gene            414..902
                     /locus_tag="ACHUN9_004281"
     CDS             414..902
                     /locus_tag="ACHUN9_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0697510.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKATYNAINWPERPDA
                     AA"
     gene            889..>2142
                     /locus_tag="ACHUN9_004282"
     CDS             889..>2142
                     /locus_tag="ACHUN9_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0697511.1"
                     /translation="MPQLKGVIKTPTGEPLDGATITLTSIHNRAGILKSVFSHVTTQN
                     GEYDFPVLPGVYSVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESLKK
                     FEELAQQAQQSAGSAAGNARQTAQDVTAAATARDDAQRFAEKARQDATVTAENRKATA
                     EDVKSTGKNAVLSGQRAQAAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAGEKA
                     QQKSRDNLRLKSAATMEAQSDIYDRTKGRLAIPGAFGFGRAFLPEDVIRFDTKSDFLA
                     WVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGP
                     IYHTRYCYWPISRLTDWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEAD
                     GDKVIRLVRGQSYRGSRLGPVGISVP"
BASE COUNT          584 a          528 c          590 g          440 t
ORIGIN      
        1 ctggatgttt atgccaatag cgtgcatgtg ttgcgttttc agagtggcag tatccagagt
       61 aataaagctg taaacgttac aggacgggta acaccgtcag actacggaaa ctttgatgcc
      121 cgttaccagc agcgaaatgg tggcgtgcag gatgtgcggt atggttatga aatgtattac
      181 acccccggca gtaacaccgt ttcgtggaca tttcgctcac cttcgggaca cgggctgtca
      241 gggatatcga tatcggatac cggccgtaac tcagcggata acgtcaacgg cgtgtattac
      301 cgaccgctgc aaaaactgat taatggcacc tggtataacg tagcgagtat ttaacaatgt
      361 tgcatttaaa aaatattacg gcaggtaatc cgaaaaccgc ggaataatat cagctgacaa
      421 aacagtatga tgtcacctgg cttttttcgg aagacggcaa aaactggtat gaagaacaga
      481 agaactttgc cagtgacacc ataaaaatgg tttacaccgg agacgggcgc gtggtgtggg
      541 tcggtaagga tgtgacaggc attgaacccc gtaacgccag tgttattgaa gttcctgata
      601 ttaccgccaa ccgccgcatt accgcgccgg gttactggtt ttaccgcaat gatgaatttg
      661 tctttgacta cagactcaaa gcggaagatg agcgtgatgc ccttctggct caggtcagtg
      721 cccggacagg ggaatgggaa gaagacctgc tgctggggct aatcagcgac gaagaccggg
      781 aaaaactgaa agcgtaccgt atttacgcga aatcgctgca ggcgatggat ttcagcacca
      841 tcacagataa agccacatat aacgccatca actggccgga gcgtcccgat gccgcagctt
      901 aaaggtgtga ttaaaacgcc cacgggagaa ccgctggacg gcgcaaccat tacactaacc
      961 tccatacaca accgtgcagg gattctgaaa agtgttttca gccatgtcac cacacagaac
     1021 ggggaatatg acttccccgt tctgccgggt gtgtacagcg ttcgcctgac acaaagcaca
     1081 cggcgccttt cagaaatcgg tgtcatccgc gtttatgaag attcaacgga cggttcgctg
     1141 aatgattttc tgggcgcaac taatattgac ctgcgtccgg aatccctgaa gaaattcgaa
     1201 gagctggcgc aacaggcgca gcagagtgcc gggagcgcag ccggaaacgc acggcagacg
     1261 gcgcaggatg tgacggcagc cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa
     1321 gcccgacagg atgcaaccgt cacagctgag aacagaaagg ccacagcgga agacgtgaaa
     1381 agcacgggga aaaacgccgt cttatccggt cagcgtgcac aggctgccgc aggttatgcc
     1441 cgcgcagcag aacaggccaa aaatgacatt gatgctgcgc tgactggcac tctgaaaatg
     1501 gctaaccatc tgtcagaaat cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat
     1561 aatctgaggc tgaaaagtgc ggccacgatg gaagcacaga gcgacattta cgaccggaca
     1621 aaaggccgtc tggcgatacc cggcgcattc ggctttgggc gtgcttttct gcctgaagat
     1681 gttatccgtt ttgacactaa gagtgatttc ctggcctggg taaggaatgc gctgccaggt
     1741 gagtattccg ttgctggccc ctacgacatc atcatacccg acacacggtt tgaaggggtg
     1801 ctcagcatcc ggtggactga tgcacgccct gagacaacag aaccgcggta cagagccaaa
     1861 tcccttactt tttacggcat taacggcccc atttatcaca cccgctactg ctactggccc
     1921 atatccagac tgactgactg ggtgaaaata aatataacca cagaagatat tatttacaga
     1981 atcgtggcga gctctgtccg caacagatgg ggagaccctg acattggcgg gctgattatt
     2041 gctgcgtacc agggagaagc tgacggtgat aaagtcatca gacttgtcag ggggcagtca
     2101 tacagaggct cacgactggg accggtgggg atttcagtgc cc
//
LOCUS       DAZOYS010000350         2091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOYS010000350 DAZOYS010000000
VERSION     DAZOYS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.353"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(266..660)
                     /locus_tag="ACHUN9_004283"
                     /pseudo
     CDS             complement(266..660)
                     /locus_tag="ACHUN9_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            603..1109
                     /locus_tag="ACHUN9_004284"
     CDS             603..1109
                     /locus_tag="ACHUN9_004284"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0697512.1"
                     /translation="MLEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHA
                     DSYGYRPGKSAHDALKQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWV
                     ILYCRRWMEAPMQSCENGELITRTRGTPQGGVISPLLANLFHHYAFDLWMEREYRGYR
                     LRGTLTIL"
     gene            1124..1651
                     /locus_tag="ACHUN9_004285"
     CDS             1124..1651
                     /locus_tag="ACHUN9_004285"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR0697513.1"
                     /translation="MSDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSF
                     TFLGYDFKVRTLKNFKGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARR
                     YNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEY
                     PKLFVHWYLLRASNE"
     gene            1734..1913
                     /locus_tag="ACHUN9_004286"
                     /pseudo
     CDS             1734..1913
                     /locus_tag="ACHUN9_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1958..2091)
                     /locus_tag="ACHUN9_004287"
                     /pseudo
     CDS             complement(1958..2091)
                     /locus_tag="ACHUN9_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331380.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          581 a          460 c          577 g          473 t
ORIGIN      
        1 agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
       61 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      121 aagtaaactg tccgatgaag catcgaaatc ttagatgatg tcaaaatggg gccgtacagc
      181 cgccccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      241 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      301 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      361 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      421 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      481 gtcgcaacaa gggagccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
      541 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
      601 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
      661 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
      721 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
      781 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
      841 ttttcgacca tgtgagacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
      901 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
      961 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     1021 ctaacttgtt ccatcactat gcatttgatt tgtggatgga aagagaatat cgggggtacc
     1081 gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag
     1141 gctgaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa
     1201 aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt
     1261 tctcggatat gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa
     1321 atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg
     1381 gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat
     1441 agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact
     1501 gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa
     1561 ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca
     1621 ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac
     1681 gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt
     1741 ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca tgccgtctga
     1801 acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggctgg
     1861 caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa
     1921 tacggttctg acgagccgct tacttttggc gtaaatctgc tgcgccttta ctgaacacaa
     1981 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     2041 ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt t
//
LOCUS       DAZOYS010000351         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOYS010000351 DAZOYS010000000
VERSION     DAZOYS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.354"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUN9_004288"
     CDS             complement(60..1025)
                     /locus_tag="ACHUN9_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0697514.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUN9_004289"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUN9_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697515.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOYS010000352         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOYS010000352 DAZOYS010000000
VERSION     DAZOYS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.355"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUN9_004290"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUN9_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0697516.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHUN9_004291"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHUN9_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0697517.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZOYS010000353         2066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOYS010000353 DAZOYS010000000
VERSION     DAZOYS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.356"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            247..1116
                     /locus_tag="ACHUN9_004292"
     CDS             247..1116
                     /locus_tag="ACHUN9_004292"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0697518.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUN9_004293"
     CDS             complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUN9_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0697519.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1881..>2066)
                     /gene="rclB"
                     /locus_tag="ACHUN9_004294"
     CDS             complement(1881..>2066)
                     /gene="rclB"
                     /locus_tag="ACHUN9_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0697520.1"
                     /translation="FSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWKV
                     TSMRIDNNSTATAVLYK"
BASE COUNT          594 a          415 c          461 g          596 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaggg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
     1081 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
     1141 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     1201 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     1261 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     1321 tttaagaatt tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     1381 atctttcaat accaagcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     1441 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     1501 ggtatatgcc atcagtcctc ccaataaacc taaccagcga ttgacaggat tagccaaaac
     1561 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     1621 ggccgattgc catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt
     1681 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     1741 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc
     1801 aatactcaga cgaatcaatg acaggccaat tttatctccc cgacttaaca ggtgcaggta
     1861 tttttccatt gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     1921 attcgcatcg atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg
     1981 gtatcctcca gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttcagaatt
     2041 attttatcat ctgcattggt ggaaaa
//
LOCUS       DAZOYS010000354         2056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOYS010000354 DAZOYS010000000
VERSION     DAZOYS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.357"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUN9_004295"
     CDS             <1..122
                     /locus_tag="ACHUN9_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697521.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUN9_004296"
     CDS             119..376
                     /locus_tag="ACHUN9_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697522.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUN9_004297"
     CDS             376..750
                     /locus_tag="ACHUN9_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0697523.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..1601
                     /locus_tag="ACHUN9_004298"
     CDS             747..1601
                     /locus_tag="ACHUN9_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0697524.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTRLIF
                     PESVVNSS"
     gene            1704..1779
                     /locus_tag="ACHUN9_004299"
     tRNA            1704..1779
                     /locus_tag="ACHUN9_004299"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1737..1739,aa:Ile,seq:cat)
     gene            1880..1956
                     /locus_tag="ACHUN9_004300"
     tRNA            1880..1956
                     /locus_tag="ACHUN9_004300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1914..1916,aa:Arg,seq:tct)
BASE COUNT          559 a          437 c          571 g          489 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat gggaaaaaac agttaagaaa ttctatgatg
     1501 cgctggtgac ccggtttgat attgaagaag catgggctga gcggcagtta aaaaaggtaa
     1561 ctaggttgat ttttccggaa tctgtggtaa attcgtcata actatgggcg ttttatgcct
     1621 gacgttagaa gagtttctac aacccgccgc cgagcgggtt ttttattgcg gaattaatta
     1681 cggaccgtta ttattctgct cccggccctt tagctcagtg gtgagagcga gcgactcata
     1741 atcgccaggt cgctggttca aatccagcaa gggccaccat cacaaaccgc cattagctta
     1801 tcaggaagag cagacgacat gataacaggg ttgttggtgc gggggcgggt ccccgatggc
     1861 ggtccattat cggtattcag cgttgttagc tcagccggac agagcaattg ccttctaagc
     1921 aatcggtcac tggttcgaat ccagtacagc gcgccatatt cattcttcca gattccttcc
     1981 ggcagagcct tatactgaaa tatacctggc tcaggatatt gttgaaaata ttttatgttt
     2041 gtcaaaaata aaagtt
//
LOCUS       DAZOYS010000355         2052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOYS010000355 DAZOYS010000000
VERSION     DAZOYS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.358"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHUN9_004301"
     CDS             complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHUN9_004301"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0697525.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            1409..1879
                     /gene="argR"
                     /locus_tag="ACHUN9_004302"
     CDS             1409..1879
                     /gene="argR"
                     /locus_tag="ACHUN9_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0697526.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          554 a          523 c          441 g          534 t
ORIGIN      
        1 gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc
       61 aaggcgatat ctttcttgag tgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
      121 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
      181 gagaagaaac gggcgtactg accatcgcct tcaacgtagg cacattcgac aacgccttgt
      241 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
      301 agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacccgc gttctggatg
      361 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
      421 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
      481 tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatgtccag cgtggtaacg
      541 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
      601 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
      661 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
      721 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
      781 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
      841 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
      901 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
      961 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     1021 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     1081 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     1141 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
     1201 cagccaaaac aacatcaatt tgataacaat taatttactt ttaaacagaa tttgcatgcc
     1261 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     1321 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     1381 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
     1441 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
     1501 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
     1561 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
     1621 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta
     1681 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
     1741 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
     1801 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct
     1861 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
     1921 attcctctca accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttaccgc
     1981 gacatatcat tcacacaatg aatacatacg gtaaaaaaag cacattatgt aaaattcatt
     2041 atctaattga aa
//
LOCUS       DAZOYS010000356         2039 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOYS010000356 DAZOYS010000000
VERSION     DAZOYS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.359"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUN9_004303"
     CDS             <1..326
                     /locus_tag="ACHUN9_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0697527.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHUN9_004304"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHUN9_004304"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0697528.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          493 a          503 c          481 g          562 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttgacattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttc
//
LOCUS       DAZOYS010000357         2014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOYS010000357 DAZOYS010000000
VERSION     DAZOYS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.360"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            82..1215
                     /gene="ompN"
                     /locus_tag="ACHUN9_004305"
     CDS             82..1215
                     /gene="ompN"
                     /locus_tag="ACHUN9_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0697529.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1356..1790
                     /gene="uspF"
                     /locus_tag="ACHUN9_004306"
     CDS             1356..1790
                     /gene="uspF"
                     /locus_tag="ACHUN9_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0697530.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          557 a          463 c          451 g          543 t
ORIGIN      
        1 taactgaact tctcctttag gatgttttca ctccccggaa tggggataaa aggcaaataa
       61 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
      121 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat
      181 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgat
      241 cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
      301 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca
      361 tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt cgattacgga
      421 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
      481 ggtgactctt ataccaatgc agacaacttt atgactggtc gtgccaacgg cgtcgcgact
      541 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
      601 ggtaacaacg aaggtgccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
      661 catgaaaatg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
      721 gctggtgcgg catacacctc atctgaccgc accaatgacc aggttaacca tactgcggcg
      781 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
      841 ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct
      901 gttgcaaaca aaacccagaa ctttgaagtc actgcacagt accagtttga ttttggtctg
      961 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cgatggtgca
     1021 gacaacccgg caggtgttga tgataaagat cttgttaaat acgccgatgt tggcgcgact
     1081 tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa
     1141 gatgacagtt tctacgctgc caacggcatc tctaccgatg atattgtcgc tttaggtctg
     1201 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
     1261 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
     1321 ttacacttta tgcggatact aaaacagaag gttttatgaa cagaacgatt cttgtcccta
     1381 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
     1441 agattgacga tgcagaggtt catttcctga cggtaatacc ttcactgcct tactatgcct
     1501 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
     1561 aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc
     1621 atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag atccccgctc
     1681 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg
     1741 ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc
     1801 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
     1861 tccgtaccat acacgccaca gttttatatc agatttctta tgctgggcgt tccggcatga
     1921 cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag
     1981 aaattagtaa gaaagaacaa tacaacctga acaa
//
LOCUS       DAZOYS010000358         2011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOYS010000358 DAZOYS010000000
VERSION     DAZOYS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.361"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHUN9_004307"
     CDS             complement(<1..264)
                     /locus_tag="ACHUN9_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR0697531.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDF"
     gene            complement(279..557)
                     /locus_tag="ACHUN9_004308"
     CDS             complement(279..557)
                     /locus_tag="ACHUN9_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0697532.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            653..964
                     /locus_tag="ACHUN9_004309"
     CDS             653..964
                     /locus_tag="ACHUN9_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0697533.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            1053..1991
                     /locus_tag="ACHUN9_004310"
     CDS             1053..1991
                     /locus_tag="ACHUN9_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0697534.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
BASE COUNT          521 a          442 c          520 g          528 t
ORIGIN      
        1 gaaatcctgc ccgaactgat tgtcgccaca gctcagtgca aaaatgcggt ttccggtaaa
       61 cggattgcgt gggcatttgt ggatcacgat tccagctttc tcaatcagcc aggcgtgctc
      121 gccgatttgt tttactgggt agccatccgg cgttgcgtgt gtatcactca ggctgtagcg
      181 gatgttgctg cgtgatgcac tggtagtgaa acggttagcg tggcgttctg ttccggtaca
      241 aaaattacgg cgttgcttca gcataaaatg acacctcgtt attttgtcat ctgcacgtat
      301 ttctctgcgt ttctgatggt tttcaggaaa attgcgaaga gatttacttt gcgttttgtg
      361 tttcttcctt cttgagtaac gggaattacc gatctatcag cctgccttct tacagcgaga
      421 atgctttgat ttgtgcgttt cgcataatcc tctaggcttt cttcaagtac cggtagccca
      481 tgttcattac ggtatgggta gaacgccgcc aaacgctcaa aatccgcttg ttcgtatgtg
      541 ttaaggactt ttgccatggt gtgataacct attcaatctg gtgctatttg tggctctttg
      601 tagcgtcaag tggtactcaa ctgataacca atatagtatt caggtgcaca ccatgtcaat
      661 agagatatca aagaagctaa aagcaattcg agaatctgag gggcttagtc aggcaaagtt
      721 cgcggattca ataggtattg cggttggtac ggttaagcaa tacgagactg gtattcgagg
      781 tgtgggaacg gaggttttac tgaaaatcac aatgcacccg gaatttaaaa aatacactac
      841 gtggttgatg agtaacgaaa caaatgaggc tgctgggcag atcagtcctt ctctctcccc
      901 tgatgggcca gaaaacacat cgtcttctca aaaatcccgc aagactggca cacagcccgg
      961 ctaatcatgg aacgctgggg gcatggtggt cttgtaacgc tggggcttca cgaatgagca
     1021 taaaatcaat tccgggaggg tatcttcttg acatgcgtcc tgaggggcgt aaaggcaaac
     1081 gcattcgcaa aaaatttaaa acgaaatcgg atgcagtttt atatgagcgg tgggtgctgg
     1141 cgcaacagca taacaatgag tggaaaggaa actccattga tcgccgtccg ctgtcagtgc
     1201 ttattgactt gtggtggaaa taccacggcc agctaatgaa gtcagggcat aacacgcgcc
     1261 ttaaattgct gcgcttgagt gaggcaatgg atgacccgtg cgtgcataaa cttaatacaa
     1321 cgatgctcac cgagctacgt gtgtccagga tagagcaggg gatacagccc agcaccataa
     1381 atcgagagat tggggcgtta agcgcgatgt ttaccgcact catctcatcc ggccattttc
     1441 ttaacgataa ccccgttcaa ggccttaaag gaatgaaggt taacgagcgt gaaatgggat
     1501 atctgagtaa gtctgaatgt gttcagttgc tggatgcact ggctgaaaat cccgatgaac
     1561 ggctggccgt cgaaatcctt ctgtcgaccg gggcgcgatg gggcgaggta gcggcactgg
     1621 agcagcgccg tgttcttcat tgtcgaatca ctttttcaaa aacgaagaac agcaaaaacc
     1681 gtaccgttcc tatttctgaa agcctgtttg aaaagatcaa aaaacggggc gggaaactgg
     1741 tgtttccgac gctggattat tcattggttc gcgatgtcat caaaacggtc gcacctgatg
     1801 ttcctgacgg ccaggctgtt catgcgctgc gccacacctt cgccagtcat ttcatgatga
     1861 acggcggcaa tattctgacg ctccagaaaa ttctggggca cgcaaagatt cagacaacga
     1921 tgatttatgc ccatcttgcg ccggattact tgcaggatgc ggtgaggttt aatcccttgg
     1981 gaggaatgta actatggaaa taaaaaaacc t
//
LOCUS       DAZOYS010000359         2002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOYS010000359 DAZOYS010000000
VERSION     DAZOYS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.362"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..387
                     /locus_tag="ACHUN9_004311"
     CDS             <1..387
                     /locus_tag="ACHUN9_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697535.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(412..750)
                     /gene="maoP"
                     /locus_tag="ACHUN9_004312"
     CDS             complement(412..750)
                     /gene="maoP"
                     /locus_tag="ACHUN9_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0697536.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            869..1708
                     /gene="hdfR"
                     /locus_tag="ACHUN9_004313"
     CDS             869..1708
                     /gene="hdfR"
                     /locus_tag="ACHUN9_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0697537.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1803..1878)
                     /locus_tag="ACHUN9_004314"
     tRNA            complement(1803..1878)
                     /locus_tag="ACHUN9_004314"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1843..1845),aa:Trp,seq:cca)
     gene            complement(1887..1963)
                     /locus_tag="ACHUN9_004315"
     tRNA            complement(1887..1963)
                     /locus_tag="ACHUN9_004315"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Asp,seq:gtc)
BASE COUNT          528 a          483 c          486 g          505 t
ORIGIN      
        1 ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg
       61 ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga gcgccaattt
      121 aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca attctgcaaa
      181 cttgagagcg aagatgcgat gtggttggaa ggaacactga tccatctggg gttatcgatt
      241 cgtgcctggc agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac
      301 attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc
      361 atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcatc
      421 agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa
      481 acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg cttctgtcac
      541 tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc
      601 gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc
      661 tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat tgtcgaaata
      721 tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct
      781 agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat
      841 aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga
      901 agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc
      961 agtgagcttt cgaatcaggc aactggaaaa tcaactgggt gtgaaccttt tcacccgcca
     1021 cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct
     1081 catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac ataacgagtt
     1141 ttctatcggt gccagcgcct cgttgtggga atgtatgctt aatcagtggt tgggacgctt
     1201 gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca
     1261 gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc
     1321 caaaatggac gaatttagta gtcagttgct ggggtatttc actttagcgc tttataccag
     1381 tgccccttct aaactaaagg gagatcttaa ctatctgcga ctggagtggg ggccagattt
     1441 tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt cccattctga caaccagttc
     1501 tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgttag
     1561 ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc
     1621 gctttatgcc atatggctgc aaaatagcga taaaaatgcg ttgattcgcg atcttttgaa
     1681 aattaacgtg ctggatgaag tgtattaatc tgaatggctg gcaaggatgc cggtagaagg
     1741 atttacttcg gagaggatta tttcagataa aaaaatcctt agctttcgct aaggatgatt
     1801 tctggcaggg gcggagagac tcgaactccc aacacccggt tttggagacc ggtgctctac
     1861 caattgaact acgcccctaa ttagggtggc ggaacggacg ggactcgaac ccgcgacccc
     1921 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
     1981 ccaccggttt tatgaccggc tt
//
LOCUS       DAZOYS010000360         1990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOYS010000360 DAZOYS010000000
VERSION     DAZOYS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.363"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            32..772
                     /locus_tag="ACHUN9_004316"
     CDS             32..772
                     /locus_tag="ACHUN9_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0697538.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            807..1990
                     /gene="htrE"
                     /locus_tag="ACHUN9_004317"
                     /pseudo
     CDS             807..1990
                     /gene="htrE"
                     /locus_tag="ACHUN9_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          563 a          428 c          437 g          562 t
ORIGIN      
        1 ctgttttaat taactctgtc agggatggtt tatgtttttt aacaccaaac atacaacagc
       61 tttatgcttc gtaacctgta tggcttttag ttcatcgtct attgcggaca ttgtcatttc
      121 gggtactcgc gtaatatata aaagcgatca aaaaagtgtc aacgtacgtc tggaaaataa
      181 agggaataac ccgttgcttg tccagagttg gttagatact ggcgataaca acgctgagcc
      241 tggcagtatt acagtccctt ttactgccac gccgccagta tcgcgtattg atgccaaacg
      301 tgggcaaaca atcaaattaa tgtacacagc cagcacctca ctgcctaaag acagagagag
      361 cgtgttctgg tttaacgtac tggaagttcc accaaaacca gatgcagaaa aggtcgcgaa
      421 tcaaagtctg ctgcaactgg catttcgcac acgtataaaa cttttctatc gcccggatgg
      481 attgaaggga aatccctctg aagccccgtt agccctgaag tggttctggt caggttcaga
      541 aggtaaggcg tcattgcgag tgaccaatcc aaccccttac tacgtctctt ttagcagtgg
      601 tgatttagaa gctagcggta aacgctatcc gattgatgtg aaaatgattg caccatttag
      661 tgatgaggtc atgaaagtca ctggccttaa tggcaaagca agttctgcaa aagtacattt
      721 ttacgccatt aatgattttg gtggcgccat tgaaggtaat gccagtctgt aatcaggcaa
      781 ggatataatt ccgcaggaag cataacgtga ctataaaacc aactaaaaat tatcataatc
      841 atctgacccg tatcgctacg ttttgcgcgc tgctgtattg caatagtgct ttctgtgctg
      901 aacttgttga atatgaccat accttcctga tggggcagaa tgcctcaaat attgatctca
      961 gccggtatag tgaaggtaac cctgctatac cgggtatgta cgacgtcagt gtttatgtca
     1021 acgatcaacc tattattaac caaagcatta catttattga aattgaagga aaaaaaacgc
     1081 acaagcctgt atcacactta agaatttatt gcagtttcat ataaattctc ccgatataaa
     1141 taacgaaaaa gccgttctgc ttgccaggga tgaaacactc ggcaattgcc ttaatttgac
     1201 ggaaattatc cctcaggctt ctgttcgtta tgacgttaac gagcaacgtc tggatataga
     1261 cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat gttgatccat cgttatggga
     1321 aaatggcatt aatgcggcca tgttgtcata caacctcaac ggatatcata gtgaaactcc
     1381 tggtcgaaga aatgacagca tttatgctgc ttttaacggt gggatgaatt taggtgcatg
     1441 gcgactgcgt gcctcaggca actacaactg gatgaccgat tctggcagta attatgattt
     1501 taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt tctcaactca ttcttggtga
     1561 gtcttataca acgggcgaaa cctttgattc tgtcagtatc cgcggcattc gtttatacag
     1621 cgacagccgc atgttgcctc cgacattagc gagttttgcg cctatcattc atggcgttgc
     1681 taataccaac gccaaagtca ctattacgca aggtggctat aagatttatg aaacgactgt
     1741 gccgccaggc gctttcgtca ttgatgatct gagtccgtca ggttacgaca gcgatcttat
     1801 tgttactgtc gaagaatccg atggctcaaa gcggacattc tcgcaacctt tttcatcggt
     1861 cgttcaaatg ttacgccctg gcgttggacg ttgggatatt agcggcggcc aggtcttaaa
     1921 agatgatatt caggatgagc ctaatttatt tcaggccagc tactactacg gtctgaataa
     1981 ctatctaacg
//
LOCUS       DAZOYS010000361         1987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOYS010000361 DAZOYS010000000
VERSION     DAZOYS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.364"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            46..546
                     /locus_tag="ACHUN9_004318"
     CDS             46..546
                     /locus_tag="ACHUN9_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0697539.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            566..760
                     /locus_tag="ACHUN9_004319"
     CDS             566..760
                     /locus_tag="ACHUN9_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0697540.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            760..1050
                     /locus_tag="ACHUN9_004320"
     CDS             760..1050
                     /locus_tag="ACHUN9_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0697541.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            1330..1482
                     /locus_tag="ACHUN9_004321"
     CDS             1330..1482
                     /locus_tag="ACHUN9_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0697542.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            1494..1859
                     /locus_tag="ACHUN9_004322"
     CDS             1494..1859
                     /locus_tag="ACHUN9_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697543.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT          603 a          443 c          461 g          480 t
ORIGIN      
        1 tgatgctatt tcaaagtgat tagcaatctt attagaattg gatgtatgaa aagaaaatca
       61 ttgtcagaca ccgacctcaa cgccgccaga agactgaaag aaatctggat ggcaaagaaa
      121 tctcagttgg gattgactca ggaacgtgcg gcggaaatta tggggttttc aacacaaggg
      181 gcggtaagtc actacctaaa tggtcaaacc ccactaaacc ttgaggctgt tctaaagttt
      241 gctggcttac tgaaagttcc ccctgagtca atcaggccag acatggtaga tttgctgcaa
      301 attgcaagga tgtattgtcg agaaccacag caagataaca tagtcgcatt accagcagac
      361 acagaacaaa cagaggatga actaccgttt gccgtagagc cgatggaacg agatttaatc
      421 catacgttca gagcttttcc gaaagaagat caagaacaca tgcttcagga gatgaaagat
      481 aaaaaagaat caatggatcg tacggttgcg cggtggttag ctgcacaaaa aggccgtcgc
      541 gcctaaatca gaggagatat gtaaaatgag tacagtcctt tccccaatgg tttcagaatt
      601 tgagacagtt gagcaagaaa atagttacaa cgaatggctg cgggccaaag tagcagcaag
      661 cctcgcggat ccccgcccgg cgattccgca tgatgaggta atggcggaaa tggaacaaat
      721 tattgcccaa attgctgctg caactcacag aggtgaataa tgttacccgt tttatggcta
      781 ccatctgctc gtgatgattt gcgccagatc gtagcctaca tagctaaaga aaatcccgcg
      841 gcagcacgca gaatgaaaat tcgaattgaa acatccgttt tgccgctcac agagcatcct
      901 tacctgtatc ctccaagcga gcgagttctt ggtcttcgtg aaattgtaac tcatcctaac
      961 tacatcatcc tttaccgagt aacagcatca agcatagaag tagtaaatat tgtgcattct
     1021 cgaagacagt atccaggcaa aaacagttaa ttattcccgc cgacagaacc accttagggt
     1081 ggttttttct tgccccaaca aaaaacaata gcgatgctat ttacatcaaa aaaaataatg
     1141 atgctattgt ttcaggcgtc aacccacccc gccccacaga atgcagggca atacttcgag
     1201 ttaccagaca gtggtcaggg gttaagtagc cagcccgagg cgtaagaaca tgacggcagg
     1261 gttcaatctt taactatgca gcaggttttt tgttccgcca ccccggcgtt aaggggaaat
     1321 gaggtcagca tggatactat cgatcttggc aacaacgaat ctctggtgtg cggcgtgttc
     1381 cctaaccagg atggaacgtt taccgccatg acgtatacca aaagcaaaac gtttaaaacc
     1441 gaatctggcg cgcgttgctg gttagccaga aacactgact gatgaggctg acgatggaat
     1501 ttaaagattt accgtctgat gtacagaaaa cagcagctca tacattgcat tctgtgctgc
     1561 gagaaatcgg gaaagatatt gcaagcgagc cagcaaaaga tctggcccgg aaaatcaaga
     1621 ccgctttcgt tgagctttat aatgttggca ctgactctga aactgtcgag accaagaccg
     1681 taagttcacc aatattctca cttgagccaa gcgtattatc aggtgaaata tgcaccgaga
     1741 tttccagcga actgcttccg gtaatacgag aagcaattcg ccgtcgcggt ttggatggaa
     1801 gttatgatca tgacgttctg caagttctca ggacaatggt gacttcactg gggatttgat
     1861 ccctgcgtcc tctaagcttt tgatatagcg gtcctccaag aagcggtaaa ttttgtcgaa
     1921 atcatctttt ccctctggtt tattcagagg attacaggac ccgtaagtag cggcatacat
     1981 ttcaacc
//
LOCUS       DAZOYS010000362         1979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOYS010000362 DAZOYS010000000
VERSION     DAZOYS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.365"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..74
                     /locus_tag="ACHUN9_004323"
     CDS             <1..74
                     /locus_tag="ACHUN9_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0697544.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="ACHUN9_004324"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="ACHUN9_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0697545.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1670
                     /locus_tag="ACHUN9_004325"
                     /pseudo
     CDS             441..1670
                     /locus_tag="ACHUN9_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1674..1833
                     /locus_tag="ACHUN9_004326"
                     /pseudo
     CDS             1674..1833
                     /locus_tag="ACHUN9_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          482 a          523 c          548 g          426 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgtcaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagccgca tgtatggctc atgtccggag aaaaatccac gatgtgcatg
     1501 caagagtgcc aaccgacatc accacggaag cccagcagcg tatcggtgaa ctgtatgcca
     1561 tcgaggcaga agtccgggga tgttcagcag aacagcgtct ggcggcaaga aaagccagag
     1621 ccgcgccact gatgcagtca ctgtatgact ggatacagca acagatgaaa attcattcgc
     1681 tgaaaatgga atgtctccac ggagaacact attatccatc gggaaatagt gcgggcaaca
     1741 gtgtttagtt atatcgagtg cgattacaat cgctggcgac ggcaccgttg gtgtggtggt
     1801 ctcagtccgg aacaatttga aaaccagagc ctcacttacg actgtgtcca tattatgtgg
     1861 gtaggatcaa taaagacgtt ttatatgtgt ctgcttgcag ccagaaaatt tataccctgc
     1921 aggtatcata aacatgcttg acaacttaat tagggccccc tcttcctttt atggtgtaa
//
LOCUS       DAZOYS010000363         1967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOYS010000363 DAZOYS010000000
VERSION     DAZOYS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.366"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUN9_004327"
     CDS             <1..347
                     /locus_tag="ACHUN9_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0697546.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUN9_004328"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUN9_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0697547.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUN9_004329"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUN9_004329"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0697548.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT          456 a          498 c          505 g          508 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaa
//
LOCUS       DAZOYS010000364         1957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOYS010000364 DAZOYS010000000
VERSION     DAZOYS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.367"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..921
                     /locus_tag="ACHUN9_004330"
                     /pseudo
     CDS             1..921
                     /locus_tag="ACHUN9_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            932..1369
                     /locus_tag="ACHUN9_004331"
                     /pseudo
     CDS             932..1369
                     /locus_tag="ACHUN9_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1369..1746
                     /locus_tag="ACHUN9_004332"
     CDS             1369..1746
                     /locus_tag="ACHUN9_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697549.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          647 a          314 c          491 g          505 t
ORIGIN      
        1 accattgcca gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac
       61 tgaatgcgtg actgccattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg
      121 ctccggcact cttatgacaa gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc
      181 tcaacaatgc acagtctggt catcggacag atcaagacgg atgagaaatc taatgagatt
      241 acagctatcc cagaacttct taacatgcta gatattaaag gaaaaatcat cacaactgat
      301 gcgatgggtt gccagaaaga tattgcagag aagatacaaa aacagggagg tgattattta
      361 ttcgcggtaa aaggaaacca ggggcggcta aataaagcct ttgaggaaaa atttccgctg
      421 aaagaattaa ataatccaga gcatgacagt tacgcaatga gtgaaaagag tcacggcaga
      481 gaagaaatcc gtcttcatat tgtttgcgat gtccctgatg aacttattga tttcacgttt
      541 gaatggaaag gactgaagaa attatgcgtg gcagtctcct ttcggtcaat aatagcagaa
      601 caaaagaaag agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgca
      661 gaaaagttcg ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt
      721 ctggacgtgg taatgaatga agacgactgc aaaataagaa gaggaaacgc cgcagaatta
      781 ttttcaggga tacggcacat cgctattaat attttaacga atgataaggt attcaaggca
      841 gggttaagac gtaagatgcg aaaagcagcc atggatagaa actatctcgc gtcagtcctt
      901 gcggggagcg ggctttcgta atctttccct agggagtcag ccgaatgttg ggaaggattt
      961 gacggatgaa gagaagaact gcttggccgg cggcagtacg ggaagcccgg gaggatgggg
     1021 accagaagat gagaatagcg caagggatcg tatgcagcat gaacggtata aagatgaatt
     1081 gcgacagaaa atgggaaaac ccgacgttac agattcaaat ttgaaaaata ttatagacga
     1141 tctttatcgg ccaaatgcaa aagtaggaag tggaagcaca gcagatgctg ttcgttatga
     1201 attggccaca ggtgaaaaag ttggtggtag aggacatgta ttgaaagcac aggattatac
     1261 aagggcttta caggattggt tggataagaa tccaacagca tctccagggg atcgagcagc
     1321 agcagaaaac gttcttcagg atttacagaa tgctttggga ggcaagtaat gaaccaatat
     1381 attgctagtt atatagaaaa aatggcatca ttttccactg ttttgtctga gacagtctca
     1441 ttagctaatg aatactggca tccagaaacg ccaccggtgg ttatgttgtt ctctcaggtt
     1501 ggaaaaacga ttgtaaaaaa tttttatgaa atgggggata ttgaaagaga aaaaatattt
     1561 aaacatgtcg aagatggtat ggtctcgctt gatgatgaat tggccacagt tgtagctaca
     1621 gggcttatag aggcaatagt cactgcgacc gatagcaatt ctttactttg gcaagattta
     1681 agtaaatttc tgggtgacaa atcaaagtct catgcattag tgtggaaaaa ttttggtcag
     1741 gagtgatatt attcaagaag tattaagctc gctattgaga tcttaatgct tcttgagatt
     1801 aaattaaagt taggacgaag taattgcagg tggaaataaa tccatagtga cgtgtggcaa
     1861 tggctattag atgttatggt gtgatgggct ttggtgattt aagagctaag ttagattgcc
     1921 ttatgaaaaa gtatggttat atatttaatg atgtgta
//
LOCUS       DAZOYS010000365         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOYS010000365 DAZOYS010000000
VERSION     DAZOYS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.368"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1522)
                     /locus_tag="ACHUN9_004333"
     CDS             complement(<1..1522)
                     /locus_tag="ACHUN9_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0697550.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVID"
     gene            complement(1611..>1913)
                     /locus_tag="ACHUN9_004334"
     CDS             complement(1611..>1913)
                     /locus_tag="ACHUN9_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697551.1"
                     /translation="PRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYS
                     NNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          598 a          372 c          344 g          599 t
ORIGIN      
        1 tgtcaatcac atcatgatgt tgccatgttc tatctaaaat aataagtgag ttttgagtat
       61 catttttagt aaaagtaaca ccattaataa tcatcatttt tgcccagccg gggtaacgga
      121 aactgagagc aaaacgaaca gatgactcag gctcaatgag acattttacg cttaaatcat
      181 gagggtattc agttaattgt gtaattttaa ttgcaacatt attaaatgtg ccataaaact
      241 cacacgggcc ataaaataat gcagtaaaac cattgttatt ttcttgaaac atattttcga
      301 caaaagccgg tatggttctt gctgtattcg gtacgcagca gacagcagcc tcctcatgag
      361 taggggagta tttatatctg gtattaaact gattactgtc aggatgtttc ctttcatttg
      421 cttcataaca attatcagtc ttacagtaca ttatcgcgtg aagtatggga tgctgcatgc
      481 caaaactagc attatagaat agccactcag catcatcggc cagaccaata ttagtggtca
      541 gttttattaa cataacataa gtgctaaata gttcatgaac agaacaatat tcatagcctg
      601 tataagtggc attagctgtg cgtcctgtaa tccactcatc accaatggga gcgccagatg
      661 gtgtaagata atatttcaac tgggataaaa aaatatctaa tgcagtttga tactgcggcc
      721 gttggctcgc tgcgatggct aatgccctaa tatgctcata cgtatgtacg ccatgacctt
      781 taagaaggta accagggtct atgacatttt tatattgcag atcttgctcc gacacgcatc
      841 ctgcggaata atcttcatac agccacaagc aatatgagag atattcaatg ttattggtta
      901 tttcataaag gcggtagaaa acatctgtta ttgttaatcc gtggctatgt ccagcaaaac
      961 cctgttccac aacgaaagga tgtgacgttt ctttagggta gccagccata atgcattgtg
     1021 cggcttgggt aagagaagtt aataaactct cattacgggt ggactcataa catccaagca
     1081 caaaacgcaa ggcagtagct tgtgcccaga actctccgtt ttctgcttta cagttaaatt
     1141 ttaatgatga atcataaatt ccaatgtagc catcatcatc ttgcgtatcg ataagttcca
     1201 aacataaatc gctcgctttt tcctgccatt ctttattatc taacaagtag gcactgcggc
     1261 aatatccatc cttccaattc gattgagttt cactatccca ccaataatat tgcatcatgt
     1321 tatcatcggg attattctgt tttttaataa cgccatcttc agcagataca gtgcgtggag
     1381 ttatacgatc tcttccatat attttatgct ccgtaataag atggggaact aatttatcca
     1441 gttctcctat atgaccgttc atataccatt ccatcttatt ctttaaccaa ccagtgggct
     1501 ttatatcgtt aaaaaaaatc ataaatacac ctttgcataa aaaagtttag acgttataaa
     1561 agacaaatag attgtgatcg cctacagacg atcacaatga aaggaaaata ttaacggttt
     1621 ttgctatcac tcaaatgttt ttcatcctgc accatatcat tgccagattc atgcaaagta
     1681 tttattaatg cattggcatc tggatattgt tgctgatact tggttagata tatatcacta
     1741 tggatatgtt cggcaattat attgataagc aagcgattat tcgagtataa ccccagtgat
     1801 tcagctcgtt gtgaaaaaac cattactgtc ttcatatcaa caacaagcat aaaacggtga
     1861 gaagggtaat attcttcttc atggttaaag cggtaataca gttctacacg tgg
//
LOCUS       DAZOYS010000366         1884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOYS010000366 DAZOYS010000000
VERSION     DAZOYS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.369"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            199..702
                     /locus_tag="ACHUN9_004335"
     CDS             199..702
                     /locus_tag="ACHUN9_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0697552.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(781..1032)
                     /gene="yecJ"
                     /locus_tag="ACHUN9_004336"
     CDS             complement(781..1032)
                     /gene="yecJ"
                     /locus_tag="ACHUN9_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0697553.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1147..1233)
                     /gene="azuC"
                     /locus_tag="ACHUN9_004337"
     CDS             complement(1147..1233)
                     /gene="azuC"
                     /locus_tag="ACHUN9_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0697554.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1496..1819
                     /locus_tag="ACHUN9_004338"
     CDS             1496..1819
                     /locus_tag="ACHUN9_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0697555.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          495 a          440 c          413 g          536 t
ORIGIN      
        1 aaatgtaaaa atatataaac ttgatgattt aagcattttc ttatacccgt tcagacgtta
       61 ttcttatttc agatcatcgt cagaattgac cccacgatca catttcggac cggcagaaag
      121 gaattattct gcaaacagta attatggtgt tttgatttat cttgcacctc tccacttctg
      181 gatataagga tattaggtat ggcaaccgct ggaatgcttc tcaaactcaa ctctcaaatg
      241 aaccgcgagt tttacgcatc caatctctac cttcacctga gtaactggtg ttctgaacag
      301 agtctgaacg gcaccgccac tttccttcgc gcccaggcac agagtaatgt gacccaaatg
      361 atgcgcatgt ttaactttat gaagagtgtc ggcgctaccc ccatcgttaa agccattgat
      421 gttcccggtg aaaaactgaa ctctctggaa gaactgttcc aaaaaacgat ggaagaatac
      481 gagcaacgtt ctagtacgtt ggcacagtta gccgatgaag cgaaagaact gaatgatgat
      541 tcaaccgtca atttcctgcg cgatctggaa aaagaacagc agcatgatgg tctgttgctg
      601 caaaccattc ttgatgaagt gcgcagtgcg aaacttgcgg gtatgtgccc tgtgcagacc
      661 gaccaacatg ttctgaatgt cgtgtcacac cagctgcatt gatcatcatc ggcgctaatg
      721 cattgcgccg atgaaggttt tgagaaaccg ctgcctcatc tgtttgaagc agcggttttt
      781 ttaatgggat tcaccctgtg gggtaaactt gagttcaata agcgcgatgg ctttttggat
      841 tgcccgcatg gtgaccgggt ctgcggcggc gggatggtta gtaaagtcga tattcttcag
      901 ctgactggac attttttcac gaacttcaac gggcgcgatt acatcgagaa catccagaat
      961 ttgtttgata accaactggc aagcaaccac atcagaaacc agttcctgat cggcattcag
     1021 cggctgggac atcgtaaact cctgatagca ttttgaaagc cgttatagta gcggcttcac
     1081 atcttcagcg atagtcacat ccaccgtcat caggacacaa aaaaacctgc cggagcaggt
     1141 tttttgttat cggaacatat tgcctggcgg tacgtctttg aacgtcttgc aatagttatt
     1201 gaacatactt ttcaggattt tgcgcagttt catcgcggca ctccgaccat ttgttataca
     1261 ggtgttattg tctttgcgct tataatatga caaccatcac aaaaatcaat ctttatgtga
     1321 tacaaatcac ataaataccc ctttaatgtt ataaaaatga taatcaaaaa acagcccccc
     1381 tatttctgac acctacagat ggcaagaaat agcgcctgcc aggcgtcttt tccggccatt
     1441 gtcgcagcgc tgtaacgcgt aaaatagtgc tttctcttac tcttctggct ggaccatgag
     1501 acttctgatt ctgactcttt cattaataac gctggcgggg tgtacggtta ctcgccaagc
     1561 ccatgtgagc gaagttgacg cggcaacagg tattgtacgg ttggtttatg accaggcttt
     1621 tttgcagcat gcccataccg atcgttatgt cagtcgcggg atcgccgatc gcgcatgcca
     1681 gcaggaaggc tatacccacg cggtcccctt tggtcagcca gtaggcaact gtagcctttt
     1741 tgcgggttcc ttatgtttga atacggaatt cactttgtca tatcagtgcc atcattccgc
     1801 cttccctgtc tttctctaaa tattttaatc agcgagggga tcttcgctga ttaaagaaat
     1861 agcggtaatg cgttttattc ccat
//
LOCUS       DAZOYS010000367         1781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOYS010000367 DAZOYS010000000
VERSION     DAZOYS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.370"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..573)
                     /locus_tag="ACHUN9_004339"
     CDS             complement(<1..573)
                     /locus_tag="ACHUN9_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0697556.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLAT"
     gene            581..1082
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004340"
                     /pseudo
     CDS             581..1082
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1100..1768)
                     /locus_tag="ACHUN9_004341"
                     /pseudo
     CDS             complement(1100..1768)
                     /locus_tag="ACHUN9_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          451 a          427 c          412 g          491 t
ORIGIN      
        1 cgtggcgagt ttagccacgc cagcatccag cccgaccatt gatgctgaag ggtgagccgg
       61 agtggatact tcactttctg tctgaatact gatgtaccac gtaccgcagg actggctgac
      121 agtgacattt ttcacaacac ccgtgacctg acggctattc cggtagcgca tccagcccag
      181 tttcggcaga aaaatacggc tgttttcctg atcgagctta acgccctgcg ggtagcggaa
      241 tgcatcattc tgtccccgct ttttgaatcg gggaaaagca gcccgcttcc ggaagaagtt
      301 tttgtaagcc cgctcaaggt ctttcagtga ctgttgcaat ggctgtgagg gagaatcttt
      361 aagccattgc gtttcagtgg catttttcca ctcaaccagc caggaagcca ttttcccgta
      421 agggatgtat ttattaccgg cctcatgatt ctcattctga cgtgccagag cacgattgaa
      481 aacgaaacga catgcgcctg cgaagcgcct catttcacac tcctgttgac cacctggtct
      541 taactggaat ttaaatgcct gtaatcgttt cacaggtcaa atgatacttt ggtctgtgaa
      601 aaaagaaacc gatatccggc gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt
      661 ctttgtcact agataccgac gccagatttt ttgacgcgac agaaaaacta cgcacttact
      721 tttcaaatgt atgtgcttat tttgaagctg aactggttga aatggatggc gaaccagatc
      781 acgtccattt gttaattaac taacctccca aactggcgat atccagtctg gtaaacagcc
      841 tcaaaggcgt atcgggtagg ttactgcgac gagatcgacc agatattgca gtcaggtatt
      901 actacaaagg cgttttgtgg agtcctggct attttgccag tagctgcgga ggtgtgccaa
      961 tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg
     1021 ccttatatcc ccgccctgaa ggatggggtt ttacggcgca ccggataaaa agcgccatct
     1081 aacgatggcg cgtctggaac tactttttgt taacgtcaaa catggtggca tcggtcgcca
     1141 tgtcatcaat aacttgttta atattttcga aagccattgg cgtactttcg ttattcaggt
     1201 ctttaccttc gccctgacga acgactttga taaccggttt attagtcgct gcatcaatca
     1261 gctcaccttc aaaatagagg cgagtatcca tggtacggtg gcctgtagcc atttgcgtcc
     1321 ccgcaaccac taatgcaaca ggaaccactt catagaattg cagcccttct ttgctggtat
     1381 ctacaccggt aatggcccca cggaaaatca gactacgcgg cccagcggtg gtaaccagtg
     1441 gtttacgctg cgctatcgct tctttcattt cggtgttggt atagttcaaa attttatcca
     1501 gaactttctg ccctacctag gtagaaggtt ttggcaccgg ataataagtg attgggttcc
     1561 agacgatgct gtcatatttg ctttgatcaa aactcgggtc tacccaacgt aaaacaggtt
     1621 tacctgttgc tgaggttgtt tcttttaaat cagagtaatt gtttaaaaaa ccagaatatt
     1681 tatctggctg ggtgatttta gaggcacaac cagacagagc caacaagccg cataaagctg
     1741 caactttcgc aaatgatgtg gtacgcatga atagtttcct g
//
LOCUS       DAZOYS010000368         1766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOYS010000368 DAZOYS010000000
VERSION     DAZOYS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.371"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            37..724
                     /locus_tag="ACHUN9_004342"
                     /pseudo
     CDS             37..724
                     /locus_tag="ACHUN9_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    317..433
                     /locus_tag="ACHUN9_004342"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            726..1092
                     /locus_tag="ACHUN9_004343"
                     /pseudo
     CDS             726..1092
                     /locus_tag="ACHUN9_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1095..1493
                     /locus_tag="ACHUN9_004344"
                     /pseudo
     CDS             1095..1493
                     /locus_tag="ACHUN9_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          437 a          436 c          525 g          368 t
ORIGIN      
        1 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
       61 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
      121 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga gactctgcgt
      181 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
      241 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
      301 tccttcgcct ggcttccgct tattttgcaa aggcggagtt cgaccgcctc tggaaaaaat
      361 aatgccactg ctggataagc tgcgtgagca gtacggggtc ggatcggtat gcagtgaact
      421 gcatattgcc ccgtcaacgt attaccactg tcagcaacag cgacatcatc atgataaacg
      481 cagtgcccgt gcgcagcgcg atgactggct gaagaaagag atactgcgcg tatacgatga
      541 gaatcatcag gtatacgctg tgcgtaaagt ctggcatcag ttgttacggg aaggtatcag
      601 agtggccaga tgcactgtgg cacgtctcat ggcggttatg gggcttgccg gtgttctccg
      661 gggtaaaaag gtccacacta ccgtcagccg gaaagccgtt gccgcaggcg accgcgtaaa
      721 ccgtcatcaa ggcaatatgc cgcgtactcc gggtggcccg cagcggctgg tatacgtggt
      781 gtcagcggcg gacaaggata aacacacgtc agcagttccg tcagcactgc gacagcgttg
      841 tccgcaaggc ttttacccag tccagcgtta cggtgcccca cgcctgacgg atgaactgtg
      901 tgctctggtt accactttaa cgtaaaaacc gtggcagcca gtctgcgccg tcaggaactg
      961 agcgcaaaag cctcccagaa gttcagcccg atcagttacc gtgcacacgg cctgcctgta
     1021 tcagaaaatc tgctgacgca ggatttttac gccagtggcc cgaaccagaa gtgggcggga
     1081 gacatcacgt actattacag ctcaccgact gcggggaaac acggggcgcc gggttattga
     1141 aggtgacctg tccagctact tcgacacagt acatcaccgg ctgctgatga aagccgtctg
     1201 ccgcaggatc agtgatgcac gttttatgag actgctgtgg aaaacgccgt gttgatgttg
     1261 gtctcttccg ggcggtcagt gaaagtatgc cacagggcgg tgtgatctcg ccgttgctgt
     1321 cgaacatcat actgaatgag ttcgaccagt atctgaataa acgctacctg agtggaaaag
     1381 ccagaaagga tcgctggtac tggaatcaca gtatccagcg aggccgaagt actgctgtca
     1441 aagaaaactg gcaatggaaa cctgccgttg cttactgttg ctatgccgat gactgtgtgc
     1501 cacgaaggcg cgtcctgggg acgtgagtcc ctgtgcagcc atgcggcaca agagatgagg
     1561 gaaggcccct cggtgtcggc gtgcaggcgc aggcaccaaa ccatccactg ctgctgcatt
     1621 taagagatgt ggtggtgagc gagatgggaa aaggtcggac gtgagccgat caggtatgga
     1681 tcgaagagac gaacgaaagt gaaccgctga tgatgtgtcg aaagcatata gatgatgtca
     1741 aaacaatggt ggagtcgttc ctgtgg
//
LOCUS       DAZOYS010000369         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZOYS010000369 DAZOYS010000000
VERSION     DAZOYS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.372"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            488..1231
                     /locus_tag="ACHUN9_004345"
     CDS             488..1231
                     /locus_tag="ACHUN9_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0697557.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            1314..>1760
                     /locus_tag="ACHUN9_004346"
     CDS             1314..>1760
                     /locus_tag="ACHUN9_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697558.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIA"
BASE COUNT          579 a          311 c          376 g          494 t
ORIGIN      
        1 gagtacttaa agggcttgcg ttaattgtaa cttattacgg catattacgg catggatgta
       61 tgccgtaata cggcaaaaaa agcccctgaa acccgctatt taccatcaat acggcaacta
      121 agtatagaga tacgaaatcc attccatttc attccatgta tttctttctc tatcagtatt
      181 ccaagaaact gtgacttgcg tcacgaatac tactcgtcgt gaactcctcg tagtattggt
      241 tagtaacgtt gcgtttttct cgccagtact cgaaaacccg caaggcgtta ctactcgctt
      301 tgctcgatag atgttagtac agcaaatgaa gtacctaaag tgaattggta agtacgacat
      361 gccccgccag ttccacaact ctcgctctgg tcatttccac tactatgatg ctattggata
      421 gctacataaa gtactggatc aagtaggcat tttttgtgta caaagaggga attaagagag
      481 ggataccatg aacagaaaac aattcatcca gtcgcatggt gctacttgta gtaactggac
      541 ttggagttgg tcatttgtga accacgacaa gaaaatggtt atctttggtg catgggatgt
      601 acaaaaagag caagaaagat cagtcattct gagggagcgg tggaaaacca aagttgtgga
      661 gggaaaggaa agaaaaaatc ttggatatat tcaagctatt gaacacattc aattgatagc
      721 tgaaggttat gagctttaca cgtttgatat ggagcaggga cgaaacgatg agaatagaga
      781 tgtagctgtt atcaaacggt tcactccaaa acttgaaaaa cgttaccttc ggaaagaagg
      841 aacagtttgg tatgcagact ttttacctaa tccatttcca gatgaaatta cctcacctga
      901 aaactacgct gagggtgcaa aaaagcaagt tacagtaaac tcctacgagc gtgatccaaa
      961 agcacgacag gcttgtattg accatcatgg tacttcctgc gggtgttgtg agtttaactt
     1021 tgagaaagtc tatggtgaac acgccaaggg ctttatacac gttcatcata tcaaaccgct
     1081 acacacagta ggcgaggatt acgaggtaaa tccaattact gatatggttc cactttgtcc
     1141 aaactgtcac gcaatggtac atcgcggtaa cgaagtatta agtgtcgaaa gtttaaaagc
     1201 actgttgttt aaagacagca ataaaatttg aatttatttt agacttgata cgacatactt
     1261 gaaaagttat gaatacaata tgctttaaat tagacatgta agttgagaga aaaatgagca
     1321 taacaacaat agcagctaca tataaagatg tgttaactat tgctttttcg gcgttgtcat
     1381 ttattattgc atgtacttcc ttatacttct ccggtagggc agcatggcat gatagatcaa
     1441 ggctaaaaat aacagctgac ctcgtctacg agtcttttga tgaggaacct tataaaatcg
     1501 aggtgatagt gatcaatgtt ggtaggcgtg atgcagtttt ggaagggatg ctttgccatt
     1561 atgaaaaagg cattaggagc cattcatctc caaaagagag cattgttatt aaagaaaagc
     1621 aaagaaaaaa atttgaattg gtaagctcag acttagtcac gatagataat gaatgtgata
     1681 tttgtcattt agaagatata actattttag atgtggaggg gaatgaacat aaaatcccta
     1741 actctcacag actcattgca
//
LOCUS       DAZOYS010000370         1750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOYS010000370 DAZOYS010000000
VERSION     DAZOYS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.373"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(107..608)
                     /gene="aqpZ"
                     /locus_tag="ACHUN9_004347"
                     /pseudo
     CDS             complement(107..608)
                     /gene="aqpZ"
                     /locus_tag="ACHUN9_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(727..>1750)
                     /locus_tag="ACHUN9_004348"
     CDS             complement(727..>1750)
                     /locus_tag="ACHUN9_004348"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFR0697559.1"
                     /translation="YKPAKLVLFELSEYALYAIEKELSALCDKEVLNVPVIPLLGSVQ
                     RQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAESAIESGVET
                     FVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLGSSGSVVPLF
                     EKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMGHGGDVFVLDMGDPVKIYDLA
                     KRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEELLIGDSVQGTSHPRIMTANEV
                     MLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFNPTDDICDLVWQQKKSLLSQA
                     SNVIRL"
BASE COUNT          503 a          460 c          396 g          391 t
ORIGIN      
        1 gaggatcgcc atgatttggc tgtcggtaaa ttttgatgtt ttcataaaga atctcctgcg
       61 tttatggtac gagaaaattc tacttttgac gctggttaat ttacggggtg attaccagac
      121 caatcgccag tggcgcaaag cccgcaggag cgcgtttttc tgtcgctccg gacaaaaaac
      181 gcactgagga caaactcgct cagcatacag gcgcttaacg catagccgcc aggtgaatgg
      241 aggccataac cattagccgc aaaaccgctg gttgtcgcat caaacccagc cttaccgctg
      301 gcgataccat acagcactgc cgcagcggca ataccgccga taacctgagc gataatgtaa
      361 ggtaacacgc gcgctgcagg gaagcgtcca ccggcccaca gacccaaggt caccgcgggg
      421 ttaaaatgcg caccagagat gtgcccgacc gcataagcca tggtgactac tgtcagacca
      481 aacgccaacg ccacgcccaa aaagcctata cctaactgtg ggaaagcagc agagatcaag
      541 gcgctaccac agccacccag aaccagccag aaagtgccga aaaattcggc gctaaaaggt
      601 ttaaacatac actgatgtcc tgtttgttaa agagcaggca tcagggtaaa ggcggtcacg
      661 atgtaccgcc tagcggggat ttgtaagaga ggtgaagaat tggtgtggaa ggttaaacct
      721 aagcaatcac aggcgaatga cattgctcgc ttgtgataac agcgattttt tctgctgcca
      781 aactagatcg caaatatcat cagttggatt gaatgccgct ggtgcttgta acaacaaact
      841 gcgaattcgc tcatgatcaa agtcatgaca agcttgatcc agctctttaa gtaagagcga
      901 tagatcctgc cacggtagca tcacttcgtt ggccgtcata attcgtggat gagaggtacc
      961 ttgaactgaa tcaccaatca gtaattcttc atacagtttc tcacctggac gtaatcccgt
     1021 aacttcaatg gcaatatcgc catctggatt tttatcatcc cgtacactca agccacttaa
     1081 ccggatcatg cgtttggcta agtcataaat cttgaccgga tcgcccatgt ctaagacaaa
     1141 gacatcgccg ccatgcccca tcgcccccgc ttgaatcacc aactgtgatg cttccggaat
     1201 tgtcatgaaa tagcgaataa tgtcacgatg agtcaaggta actggcccac cttgggcaat
     1261 ctgtttttca aacaacggca cgacagagcc cgaagaaccg agtacattac caaatcgcac
     1321 catacaaaag cgagtttggc tttgccgtgc agacaaagcc tgcaatacca attcggccag
     1381 acgcttagtt gtccccatag tgttggtcgg gcgcaccgct ttatcggtgg aaatcaacac
     1441 aaaagtttca acgccacttt cgatcgctga ctcagcgcag tacaaggtac caaacacgtt
     1501 attacgtacc ccttccacca cattatgctc aaccagaggc acatgtttat aagcggccgc
     1561 atgataaacc gtttgaatac caaaggactt catcaccatc tgtaagcgat tctgacgctg
     1621 caccgagccc aacagaggga tcactggaac attcaaaact tctttgtcac acagcgccga
     1681 gagctctttc tcaatagcgt agagggcata ttcagacagt tcaaatagaa ccaatttggc
     1741 cggcttatat
//
LOCUS       DAZOYS010000371         1734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOYS010000371 DAZOYS010000000
VERSION     DAZOYS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.374"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUN9_004349"
     CDS             <1..493
                     /locus_tag="ACHUN9_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0697560.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUN9_004350"
     CDS             493..1083
                     /locus_tag="ACHUN9_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0697561.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..>1734
                     /locus_tag="ACHUN9_004351"
     CDS             1070..>1734
                     /locus_tag="ACHUN9_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0697562.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTG"
BASE COUNT          433 a          381 c          511 g          409 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gatt
//
LOCUS       DAZOYS010000372         1728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOYS010000372 DAZOYS010000000
VERSION     DAZOYS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.375"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUN9_004352"
     CDS             complement(<1..356)
                     /locus_tag="ACHUN9_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0697563.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(356..850)
                     /locus_tag="ACHUN9_004353"
     CDS             complement(356..850)
                     /locus_tag="ACHUN9_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR0697564.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1208..1384
                     /locus_tag="ACHUN9_004354"
     CDS             1208..1384
                     /locus_tag="ACHUN9_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697565.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1526..1678)
                     /locus_tag="ACHUN9_004355"
     CDS             complement(1526..1678)
                     /locus_tag="ACHUN9_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697566.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1666..>1728)
                     /locus_tag="ACHUN9_004356"
     CDS             complement(1666..>1728)
                     /locus_tag="ACHUN9_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0697567.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          476 c          399 g          412 t
ORIGIN      
        1 ctggcgagtc gttttttgga gtgttcgcgg gctttttcct ccgtcatctg aataatcagc
       61 atatcagcag gatcgcaaat cacgttgtaa atcacccagc cgtcaatcag gccgatagtc
      121 ttgccggttc gtgccgggcc aacaaatatc actgcgtcgt attcacgcga ggccaggcag
      181 ttcatcggct caataacata cggtgccacc agcggatccc acgggactga gttccctgcc
      241 cccatgggca cccgcatata ctgagcaacg gcatcagcaa cccgcattcg tctcggtgcg
      301 cgaaggatat aacctgaatc ggttcgtgct gcctttgcgg tttcctgatt cagcattact
      361 cctcctgctg taattcctcc tcatcatccg cacctgcttc agtcacccgc agggctatct
      421 gatcgcgcag atcatcaata atggactgaa cacggctcac agcggcaggc tgcagaccgc
      481 agtcacgttc aagaatatcc ggtaatgtct ccagcacctg cacgaccgct tttgcccaga
      541 tggcaaactc ccgtctgaca tcactggccg gaatgagttg tgccgtttcc tgttcgaact
      601 taagacgctc acgttcagac tgataccagg ctttgcgctc atgcgcgtcc atttcgcctt
      661 ctgcaaccgg cggtggtaat gccagaaatg ccgacacaat atcaaccacc cgataaagct
      721 tgaggttgct ttcatgcccc cctgcaacgg gtagattttg cagccttgcc gcagcagtct
      781 ggcgatgtac acctgacagt gccgccagtt gactgatatt cagcgtcaga ttttttaact
      841 ctcgatccat acccgctcca gaatgtttta aacatgcatc ttgcgaacaa ctttaggcaa
      901 acggtgttag tgatgaacaa aaaacaatca aaatcgacac cataaaaata aaaccactgt
      961 aatatcaatc cattacagta gtggtgatga cgaatgaaat ttcaaaaact agcctttttc
     1021 cgcgccgctc ccgccccgtg gcaggccacc ccaccgggag gacccgtcag cctgacagcc
     1081 atgacgaacg tctgatacag cgccctgcat gaatgacatc gggataatcc agaaaggaat
     1141 agcatcgtac ccacaagaat cagtgtaagt gtcctgtttc ttccaccccc gcacaggact
     1201 ggcgagcatg agggacaaac ccgcgaacca taaacgcggt aaaaacccgg tgtgcatcgt
     1261 ttttgattat tcccgcacac tcgcgcagaa ggagttcccc gtcgggctac ggtcatggtt
     1321 aatacgggaa tacggcgacg atacagcgca tgatgtgtca ggcttgaata cctttatccg
     1381 ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc aaagccactc
     1441 tgtaggaagt ggcttttgta atggcaataa aaagccccgc gaatgcgagg ctaaatcctg
     1501 gtatttgtaa tgactggctc ttatctcaac gcagcccctt accgcgcgca aaatgctcaa
     1561 tatcaagcat cagcaatgag atgtttaatc tggattcact ccagaagtga gcaccaccct
     1621 gtctacagag ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg
     1681 cgtcctgatg tattcctgaa gcgttctcag tgctgtttgg tcgcggat
//
LOCUS       DAZOYS010000373         1712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOYS010000373 DAZOYS010000000
VERSION     DAZOYS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.376"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..415)
                     /locus_tag="ACHUN9_004357"
     CDS             complement(<1..415)
                     /locus_tag="ACHUN9_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0697568.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
     gene            complement(431..1105)
                     /locus_tag="ACHUN9_004358"
     CDS             complement(431..1105)
                     /locus_tag="ACHUN9_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0697569.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(1185..>1712)
                     /locus_tag="ACHUN9_004359"
     CDS             complement(1185..>1712)
                     /locus_tag="ACHUN9_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697570.1"
                     /translation="IAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDS
                     VSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGND
                     KAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQ
                     SGDVLSSLVMRVAQD"
BASE COUNT          484 a          384 c          310 g          534 t
ORIGIN      
        1 tttccagttc ttccacccag gcctgcggga cactgaaatc gagacgaaaa accccgatat
       61 cccaggtata gctcccaccc tgaacaagtt gctcaaatgt taaacattgc ttaccgctgg
      121 cgaagttatc gctattaatg cctaaccgct tgataacttc tcttgataaa catgtttctt
      181 gcgggttgtc tttaacaata atctcatatt tcccgcgcca ttgcttattg acataaatat
      241 cgatgtcata ctgccccggt aagggttgat tatcatcaag acgaatattt gcaacctgct
      301 ggtctttcat tccacctatc ataaaatggg tatcaaaggt ttcttcagct gcataagctt
      361 caatgccgag caataacgct acgattgctg atgcaagtgg ggtcattctc aacataggca
      421 gctcctgcaa ttaaattttg tcactaatat agttgccatg gtcatcgata atggtcagat
      481 gccagttatt tgcattatta cttttgacat taacactctg actttctaag ggggcaatca
      541 taatagtttc ataattgact ttgacattat tagctttgac atccgaaatc gtcacccaat
      601 tagctgagtc attttttatc accaaaccat tgccactgcg atttaccagc aattttttaa
      661 atgtcgcttt atttaccgga gcaatacccg ctggtcggta aaacaactta attctgtttt
      721 gcatcgcaaa cttcagtgca ttcttacctt cttgctctgg actatttggt ggaatatcca
      781 gaacattcag ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga
      841 tttttacttg ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag
      901 gaacctgaat tttttctggt ggtaatgacg tatcgccatc atcaatccac gcctgcagca
      961 gcgaagaacg gtttccctgg ttcatcaact gcaccatcac ttctttattt tctgccgggt
     1021 aaataatgcg cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa
     1081 tgagtaaaga taataatcct ttcattttaa tgcctcttaa attaaaaggc cgccatggga
     1141 tagcggccat gtttattacc tggagaaact tacaatttta tttattaatc ctgcgccaca
     1201 cgcataacca gagaagataa cacatcacct gattgcactg agttatctgt cagtttcagg
     1261 tattttgctt taaacggaat agcatattga ccttccgctg ttttataact ctcaatgtta
     1321 aaaggtgcag aaacatccag gacatcgctg ttatcaccta aatagagata gtaacccacg
     1381 gtatcgttgc tactggctat cgccttatcg ttaccggtta ttttatcgtt ggtaatcaac
     1441 gagagtttta caccagcaac ttctttcggg cagctaattt ttaacatgct gctggttaca
     1501 ccgactgcat catcataggt agaatattta gctttacctt caaagacagt ggttggtaca
     1561 gtcggcatat taatacttac tgaatcacca cccgcttcga atttacacgg agattctgca
     1621 atattcccct taatagttaa tgttccggta tcaacgtctg cagcaaatac tccagcgctc
     1681 ataaaaaaag aagaaaagac agcggcagca at
//
LOCUS       DAZOYS010000374         1691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOYS010000374 DAZOYS010000000
VERSION     DAZOYS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.377"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="ACHUN9_004360"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="ACHUN9_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0697571.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="ACHUN9_004361"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="ACHUN9_004361"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0697572.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          439 a          418 c          415 g          419 t
ORIGIN      
        1 agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcaaacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 aggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc
      601 atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt
      661 atggtgaagg cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac
      721 tttcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag cagcctgtcc
     1141 agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg ctcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat
     1621 catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc a
//
LOCUS       DAZOYS010000375         1682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOYS010000375 DAZOYS010000000
VERSION     DAZOYS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.378"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            62..1371
                     /gene="ltrA"
                     /locus_tag="ACHUN9_004362"
                     /pseudo
     CDS             62..1371
                     /gene="ltrA"
                     /locus_tag="ACHUN9_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1434..1682
                     /locus_tag="ACHUN9_004363"
                     /pseudo
     CDS             1434..1682
                     /locus_tag="ACHUN9_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          416 a          392 c          513 g          361 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcgac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcagaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaact cgtgttatgg ctgccccgta aacacaagcg acttaggggc
     1261 caccgacgac gggccagcca ctggctggcc cgtgttgcgc ggagtgaaac gaggttgttt
     1321 gcacactggc cgttactatg gggacaggcc tcaatgagaa gagccggatg agctgagagg
     1381 ttcacgtccg gttccgtgag caccagaggg ggaggttccc tcgggtgact cgacttgtag
     1441 tacattgttc acgaatgagt gatgcaacaa ggctgaagaa caggttatcg gtgcgcttct
     1501 cggaagtcgg gttggtcctg aacgcaggga agacgaatat cgcctacatt gacacgttta
     1561 aaaggagaaa cgtcgctacg agtttctcct ttctcggata tgacttcaaa gtgcgtactc
     1621 tgaagaattt caaaggcgaa ttgtacagaa aatgtatgcc gggtgcatca aatgcagcaa
     1681 tg
//
LOCUS       DAZOYS010000376         1658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOYS010000376 DAZOYS010000000
VERSION     DAZOYS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.379"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..789)
                     /locus_tag="ACHUN9_004364"
     CDS             complement(<1..789)
                     /locus_tag="ACHUN9_004364"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0697573.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNA"
     gene            778..1658
                     /locus_tag="ACHUN9_004365"
                     /pseudo
     CDS             778..1658
                     /locus_tag="ACHUN9_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1058..1174
                     /locus_tag="ACHUN9_004365"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          403 a          382 c          419 g          454 t
ORIGIN      
        1 tgcattatct aagcagttac cacgtcggga catgctttgt gttatgccat tatcactcaa
       61 ccaacgttgc caacgaggca tctgatattg ccacccctga tcactatgca acaagggctt
      121 atcgtcttta ttgagttgtt ctaccgcatc taatagcatg ctctttacca ttgatgctaa
      181 tgggcgctca gtaaggttat atgagactat ttctccatta tacaggtcca tgattggtga
      241 taaatagagc ttcctgccat cgaccttaaa ctctgtgaca tctgttaccc acttttgatt
      301 tgggcggtca gcataaaatt gtctcttcag taaattatta caagttcgcc cctgttctcc
      361 acgataggca cggtacttct tgcgcctgat aagtgagctg atacccagtt ccttcattaa
      421 ctttctgatg gttttaccat taagtaaaat gccctcgtta cggcaggcaa gtctgatgcg
      481 tcgataacca taacgccctt tatgctgatg gtatattctc aatattactt gtttaacaag
      541 ttgatatgga tctggcgcat taaggcgttt cacgtgatag taaaatgtac tacgtggcaa
      601 atccgcgata accagtaatt gtgataatgg atgacattgc cttaactcat taataatgat
      661 tactttttct tttgttccaa tcgtaatttt tcctgagcta aggctttcag cttttttaga
      721 taatgaaccg ccccgggaat cctggagact aaactccctg agaaagaggt aaacaggatg
      781 actaaaaata ctcgttttcc cccgaagtcc gtcaacgggc agtccgtatg gttctggaaa
      841 gtcagggcga atatgactca caatgggcgg taatttgttc cattactcca aagattggct
      901 gtacaccgga gactctgcgt gtctgggttc gccagcatga gcgggatacc aggggcggtg
      961 atggagggct caccaccgct gaacgtcagc gtctgaaaga gctggagcgt gagaatcgtg
     1021 aactgcgctg cagtaacgat atccttcgcc aggcttccgc ttattttgcg aaggcggagt
     1081 tcgaccgcct ctggaaaaaa taatgccact gctggataag ctgcgtgggc agtacggggt
     1141 cggaccggta tgcagtgaac tgcatattgc cccgtcaacg tattaccact gttagcaaca
     1201 ggacatcatc ctgataaacg cagtgcccgt gcgcagcacg atgactggct gaagagagag
     1261 atacagcgcg tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt ctggcgccag
     1321 ttgttacggg aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg
     1381 ggacttgtcg gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt
     1441 gccgcaggtg accgcgtaaa ccgtcagttc gtggcagaac gtcctgacca gctgtgggtg
     1501 gctgatttta cttacgtcag cacatggcag ggcttcgtct atgtggcgtt catcattgat
     1561 gtgtttgccg gatacatcgt gtggtgacga gtctcatcgt ctatggaaac gacattcgtg
     1621 ctggatgcac tggagcaggc gttgtgggcc cgtcgtcc
//
LOCUS       DAZOYS010000377         1653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOYS010000377 DAZOYS010000000
VERSION     DAZOYS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.380"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            51..1304
                     /locus_tag="ACHUN9_004366"
     CDS             51..1304
                     /locus_tag="ACHUN9_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0697574.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1353..1472)
                     /locus_tag="ACHUN9_004367"
     CDS             complement(1353..1472)
                     /locus_tag="ACHUN9_004367"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0697575.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          340 a          420 c          406 g          487 t
ORIGIN      
        1 ctaacctatg tataagcctg atccacaggc atatttagca aggatttcaa gtgagccgta
       61 ctacaactgt tgatggcgct ccggcaagcg acactgacaa gcaaagcatt tctcagccaa
      121 atcaatttat taaacgcggt acgccgcaat ttatgcgcgt caccctggcg ctgttctctg
      181 ccggactggc aacctttgca ctcctctatt gtgtgcagcc tatccttccg gtgctttcgc
      241 aggagtttgg cttaaccccc gcgaacagta gtatttcact gtccatttcc acggcgatgt
      301 tggctattgg tttgctgttt actggccccc tatccgatgc cattggtcgc aaaccagtga
      361 tggtcacggc gctactgttg gcctccattt gtacgttact ttcgacaatg atgaccagct
      421 ggcacggcat tttgattatg cgcgccttga ttgggctttc gttaagtggc gtggcagctg
      481 ttggcatgac ttatcttagc gaggaaatcc accccagttt cgtggccttt tcgatggggt
      541 tgtatatcag cggcaactca attggcggca tgagcggacg cttaattagt ggtgtcttca
      601 cggacttttt caactggcga attgctctgg cggcaatcgg ttgtttcgcg ctagcctcgg
      661 cgttgatgtt ctggaaaatc cttccggaat cacgccattt tcgcccgact tcgctgcgcc
      721 ctaagacgtt atttatcaac tttcgtctgc actggcgtga ccggggatta ccgttattgt
      781 tcgcagaagg ctttttgctg atggggtcgt tcgtcacgct gtttaattac atcggctatc
      841 ggttgatgct ttccccctgg catgtcagtc aggccgtggt tggcttatta tcgctggctt
      901 atttgaccgg tacatggagc tcacccaaag ccggaaccat gaccacccgc tacgggcgcg
      961 gtccggtgat gttgttttcg acgggggtta tgctgtttgg tttactgatg accttattca
     1021 gctcgctgtg gctgatcttt gccggaatgt tactcttctc agcaggattc ttcgcagccc
     1081 actcagttgc cagcagctgg attggccccc gcgcaaaacg cgctaaaggc caggcctcct
     1141 cgctgtattt gttcagttac tatctggggt cgagtattgc cgggacgctg ggtggtgttt
     1201 tctggcataa ctatggctgg aacggcgtcg gcgcatttat tgctctgatg ctggtcattg
     1261 ctctgctggt cgggacgcgt ttgcatcgtc gtctgcacgc ctgaaaaata agcccggact
     1321 gcggtaacta cacgtccgga cttattgctt gctcaaacca acgttaatag ccatcctaaa
     1381 atagacgaag cgccagccaa ttcccgcagc gcgtctagcg tcatcaggat tataagtacc
     1441 caaataaacg gattcatttt gctgtgtgtc atttattact gatgcgcagt tattctaccg
     1501 ctttgtaagt agtaaaatag ttaacccgat caagactact attattggta gctaaatttc
     1561 ccttaagtca caatacgtta ttatcaatgc tgtaatttat tcagcgtttg tacatatcgt
     1621 tacacgctga aaccaaccac tcacggaagt ctg
//
LOCUS       DAZOYS010000378         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOYS010000378 DAZOYS010000000
VERSION     DAZOYS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.381"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHUN9_004368"
     CDS             <1..94
                     /locus_tag="ACHUN9_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0697576.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            105..293
                     /gene="dicB"
                     /locus_tag="ACHUN9_004369"
     CDS             105..293
                     /gene="dicB"
                     /locus_tag="ACHUN9_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR0697577.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            290..481
                     /locus_tag="ACHUN9_004370"
     CDS             290..481
                     /locus_tag="ACHUN9_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR0697578.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            575..>1630
                     /locus_tag="ACHUN9_004371"
     CDS             575..>1630
                     /locus_tag="ACHUN9_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR0697579.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          362 c          391 g          379 t
ORIGIN      
        1 ccggttttat ctggaagaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
       61 gactgtagag caattactca aaattcattg ttgaggacgc gataatggaa acgttattac
      121 caaacgttaa tacgactgaa ggttgttttg aaattggtgt cactatcagt aacacggtat
      181 ttactgaaga tgccattaac aagagaaaac acgaacggga gtcattaaat aaaatatgca
      241 ttgtttcaat gctggctcgt ttgcgtctga tgcaaaaagg atgctggcga tgaatacagc
      301 atttgcactc gttctgaccg ttattcttaa tacgggcgaa ccagctgaac tggttaccgg
      361 agtatacaac tccatgaaag aatgcgtggc tgctgcagct gaacagaaaa ttcccggtaa
      421 ctgttatccg gttgataaaa tcattcacca ggataataaa gaagtcccgg caggtcttta
      481 aagcagccac gtaataaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      541 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      601 cattccggac gaaaaggcaa caaaagaaga aggcgctaaa cctgtagcca ctgccattga
      661 agccggcgac gaacgccgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      721 ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg gtataccccg
      781 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      841 tgcttccttt gtcccggttg agactgaatc cgaaccgatg aacgtcactt ttgacaagct
      901 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      961 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
     1021 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtgtccgg agattaaact
     1081 gcatgccatc gggtggatta agcataaatg taagcctggt gcaaaatggc cagaaattca
     1141 ggcagagatg cgcatctgga aaaaatgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
     1201 cacgtctgtt gttgatctcg cccgtgccag agccaatcaa cagcacactg aaaattcaac
     1261 aggaaaaatc agcccggcca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
     1321 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
     1381 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
     1441 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1501 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1561 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1621 cgcaactgat
//
LOCUS       DAZOYS010000379         1625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOYS010000379 DAZOYS010000000
VERSION     DAZOYS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.382"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..1436
                     /locus_tag="ACHUN9_004372"
                     /pseudo
     CDS             1..1436
                     /locus_tag="ACHUN9_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          460 a          419 c          394 g          352 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcgaactga ggtgtactgg caatagcgga
     1561 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     1621 gggga
//
LOCUS       DAZOYS010000380         1605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOYS010000380 DAZOYS010000000
VERSION     DAZOYS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.383"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..216)
                     /locus_tag="ACHUN9_004373"
     CDS             complement(<1..216)
                     /locus_tag="ACHUN9_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0697580.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDGGLTTAERQR"
     gene            314..574
                     /locus_tag="ACHUN9_004374"
     CDS             314..574
                     /locus_tag="ACHUN9_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697581.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            571..1161
                     /locus_tag="ACHUN9_004375"
     CDS             571..1161
                     /locus_tag="ACHUN9_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697582.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1181..1528)
                     /locus_tag="ACHUN9_004376"
     CDS             complement(1181..1528)
                     /locus_tag="ACHUN9_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0697583.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          371 a          449 c          457 g          328 t
ORIGIN      
        1 acgctgacgt tcagcggtgg tgagccctcc atcaccgccc ccggtatccc gctcatgctg
       61 gcgaacccgg acacgcagag tctccggcgt acagccaatc tttggagcaa tggaacaaat
      121 tgtcgcccat tgtgagtcat attcgccctg actttccaga accatacgga ctgcccgttg
      181 acggacttcg ggggaaaaac gagtattttt agtcatcctg tttacctctt tctcaggaag
      241 tttagtctcc aggattcccg gggcggttca atgattttta cctgtcagaa cattatcaga
      301 tggaacgtgc aggatggatc aggaaatgac gttttcactc agctatgaac aactgacccg
      361 gtttgcggaa aaacgcatcc gggaatgcaa tctggacagc cacggcgtaa cttatctctg
      421 cgagtcagcc aaagccggag ccgtgctgat cttctggcat gaactggcca taaatggcta
      481 tacctcaatg aatgccataa agagacagga gatcattgac gccgaccatc agcgcctcag
      541 aaagctcatc tggccggagg atgactggaa atgactcaga gccgccgacc gtcgccactg
      601 cagcggagag tgctgattgt gctggctgcc cttgatgaaa aacggccagg acccgtgctg
      661 acgcgggaca tagaacgggt gctggagcaa agcggggaag ccccggtcta cgggccaaac
      721 ctgcgcgcct cctgccgtcg tctggaggat gccggctggc tgcgtaccct gcgcgctccg
      781 aacctgcagc tggcagtgga gctgacggac gccggccggg ccgtggcaca gcctctgctg
      841 ctggcagaac aggaccgcat ccgggcagaa cagcgtgcgg cagaagtcgt cgtactgccg
      901 ctggtcccgg cagcaggtct gcccgcaggc ggcacttctg ccactgatct ggcggtggag
      961 ctgaacggca taacgtatca ggcctgccgt ggggattttg tggtgcgtct cgacggcagc
     1021 acctgtctgc agttatggaa taaagaaggc tgggtggtac gccgggaggg cgatccgctg
     1081 gaagtggcgc agtggctgca ggcctgtcac gatgcaggaa tggaggttcg cgtacagata
     1141 aatgaaagtg ccgctccgta agaacacgcc tgtgcacggt ttacacatca tcccatggtg
     1201 gcagtcggtt aactttcctg cgccaccagt ggaaagaggc catgagcagt ccggacaaaa
     1261 aaccccccgt gatacttata ataatggcct cacctgccac cattcctgac ggcccccagt
     1321 agataaacca cattgcacat ccccaggaaa tgccccataa gccccccgtc agcaccgtga
     1381 cctgccagaa cggcataaag ggtaatggcg gaagccggat acccagccgc cagagaatac
     1441 gcagaagagg aggggcataa ttgctccgcc acatcttttt gctgtccatc agggcaatgg
     1501 cccgggcttt tttctgctca aaagtcacag gagcacctcc gttccatgat gtaaacgtga
     1561 cacagcttat tgttattgat tattattatc atgctgtggt tatgt
//
LOCUS       DAZOYS010000381         1596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOYS010000381 DAZOYS010000000
VERSION     DAZOYS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.384"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>1596
                     /locus_tag="ACHUN9_004377"
     CDS             <1..>1596
                     /locus_tag="ACHUN9_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR0697584.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKN"
BASE COUNT          555 a          314 c          298 g          429 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaaca
//
LOCUS       DAZOYS010000382         1594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOYS010000382 DAZOYS010000000
VERSION     DAZOYS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.385"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            194..1237
                     /locus_tag="ACHUN9_004378"
     CDS             194..1237
                     /locus_tag="ACHUN9_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0697585.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          455 a          354 c          338 g          447 t
ORIGIN      
        1 taatttagca tttcaatcat ctgacatcat tataaaaaat gagattcaca taaagataaa
       61 attattgttc tcaaggaaag catgaagggg cttcgtaagc aatattcatt cggtgccaag
      121 acaggacttg atacacagaa tctcaacttt tatgctctca gactatatca ccacatgttt
      181 tacaggctca gaaatgaacg atagattatg ctttgaagtg cacgataaca agggttattt
      241 cgtattttcg gatacctggt ttggcccatt gctgggggag tttgaagaag tgcttgatgc
      301 atacgacgct gatgaaatca gcgagacgag ttacatcaac aaactccgac gccttgctca
      361 acgggagccg gactttattg atatacacgc tcatctggca tatgcatttc tggaacagaa
      421 tgccccgcgt aaggcactga acgcctctct taaaggactg gcggcaggta atcgtatcat
      481 cccggaaagt ttctgtggtg aaataatctg gatgcatccc gaaaaccggc cttatctgcg
      541 cgcgctttat gcagcaattc tcgcaaatgt gcacttacag cgccatcagg atgcggttat
      601 gctcactgat aaaatactgg catacaatcc tgaagataat cagggggctc gctggttgtt
      661 gggttcagag ctgttacgca ccggcgatca tgagcgagcc ttcagtgtac tgaaagagca
      721 tgctgatgaa ttttcacctt actggtatga actcggtctc ctgcattttc tgaacgggga
      781 acatgttaag gccgccacag cttttcgcca tggctttgcc acaaacactt acatcgcaga
      841 aatgctgtgc ggaaatctgc atccatttcc acttgctgtg tggcatgact tctccggctc
      901 tctggatacg gcagaagatt actatgcaac atactcacca ttatggggac aatatccgga
      961 agcattgctg tttgtaaact ggctctataa ccattcctcc gtgctgcatg aacgatcaga
     1021 aatcattaaa tgcgccgaaa tgctgattca ggaagatgat ttcgaaatat gcgaaagtat
     1081 tttaagacag caggagcacc tgtggaaaag aattgacaag acactttctg aggaaattgt
     1141 acagaaatgc agaaatatga acggtgaata tatctggccc tggatattgc ccttttccgc
     1201 agcagggatg aaacattcaa gcatccagta tcagtagata ttgcattagt gtaactttaa
     1261 aacaagtaat aatttattat ccaataataa cactgtgttc cttccccagg tgattaccct
     1321 ggggttattt tctgaaagtt tcctttgcaa aaggatagcg ttgtaaatta ctttaacgaa
     1381 ttactccatg aactcattaa agttctttcg taatacttta tcgtcaaaca cgttatagct
     1441 ggcatcaaaa accacagatt taccctgcca gcgggtaatc ccctcctctg gcgttttcgg
     1501 ttgaactggt atccccgtgg atttgcccct atatttccag acatctgtta tcacttaacc
     1561 cattacaagc ccgctgccgc agatattccc gtgg
//
LOCUS       DAZOYS010000383         1579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOYS010000383 DAZOYS010000000
VERSION     DAZOYS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.386"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..963)
                     /gene="istA"
                     /locus_tag="ACHUN9_004379"
     CDS             complement(<1..963)
                     /gene="istA"
                     /locus_tag="ACHUN9_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR0697586.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
     gene            complement(1050..1411)
                     /locus_tag="ACHUN9_004380"
                     /pseudo
     CDS             complement(1050..1411)
                     /locus_tag="ACHUN9_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1490..1579
                     /locus_tag="ACHUN9_004381"
                     /pseudo
     CDS             1490..1579
                     /locus_tag="ACHUN9_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          381 a          399 c          417 g          382 t
ORIGIN      
        1 gtcgaagctc accagatttt caccaggatg cacgtcatac tcttttttct ccggaggcag
       61 tgccagcatg gactgctgct cttcgagcca gcgatcacag ggacgggcct ggattgtttc
      121 atgctttcgt tggttagcga catcgtgcag ccagcgcaga ccgtggcggt tggctgtttc
      181 aacatcgaca gtgctcccca tcgggcgcag gcgagtcatt agtgggatgt aaaaactgtt
      241 acgggtgtac tgcaccatcc gttccacctt acctttagtc tgtgccctga aggggcgaca
      301 cagtcgggga gagaagccca tctccttgcc gaactgccac agcgaaggat ggaaccggtg
      361 ctgaccggtc tgatatgcgt cacgttgcag aaccacagtt ttcatattgt catacaacac
      421 ttcgcgcggc acaccaccaa agaagcggaa cgcattacga tggcaggtct ccagcgtgtc
      481 ataacgcata ttgtcagtga attcgatgta caacattcgg ctgtatccgg gaacagcaac
      541 gaacacgtga agcggtgagc gaccattacg catagtgccc cagtcaacct gcatctgtcg
      601 tccgggttca gtttcgaacc gaacggcagg ctcctgctcc tgaggaaccg agagagaacg
      661 aatgaatgcc ctgagaatgg tcattccgcc acgatatccc tggtctctga tctcgcgagc
      721 gattaccgtt gccgggattt tgtaaggatg agcatcggcg atgcgttgac gaatataatc
      781 ccggtattca tccaggagtg aagcaacagc aggtcgcggc gtatattttg gcggctcaga
      841 ttttgcctgc aaataacgtt taacggtatt gcgggagagc cccagttctc tggcaatcgc
      901 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
      961 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
     1021 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
     1081 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
     1141 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagtg
     1201 tcctggcggt atcacggccc cctgcgccgt tatgtgccat ttcaacgatc tgctccttaa
     1261 cgccgggttt tctggcttca taagagtaag tgagctggaa aacacgttta catgagcgac
     1321 aacggaaacg ctcatgccgg gaacaactac ggccatgtcg ataaacttca tcggaatgac
     1381 agcgcggaca atgaacagta acggtagtca tggaaaacct caaataccag tagattacca
     1441 gaaacttcta tcgattgaag acattacctt ttgccgcaat aggtggggta acattggtga
     1501 aaacgcatta cgccgggtgg cagttggaaa aaaattatct tttttcggct ctgatcgtgg
     1561 tggagaaaat gcggcaata
//
LOCUS       DAZOYS010000384         1559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOYS010000384 DAZOYS010000000
VERSION     DAZOYS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.387"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>1559
                     /gene="rhsC"
                     /locus_tag="ACHUN9_004382"
     CDS             <1..>1559
                     /gene="rhsC"
                     /locus_tag="ACHUN9_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR0697587.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGER
                     QTVHHPQTEALL"
BASE COUNT          371 a          459 c          484 g          245 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt atgacgaacg cggctggctg
     1441 acagacatca gccatctcag cgaagggcac cgggtgacgg tgcattacgg gtatgatgag
     1501 aaaggccggc tgaccggtga gcgtcagacg gtgcatcacc cgcagacgga agcactgct
//
LOCUS       DAZOYS010000385         1554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOYS010000385 DAZOYS010000000
VERSION     DAZOYS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.388"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            15..779
                     /gene="nfeF"
                     /locus_tag="ACHUN9_004383"
                     /gene_synonym="yqjH"
     CDS             15..779
                     /gene="nfeF"
                     /locus_tag="ACHUN9_004383"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0697588.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(833..908)
                     /locus_tag="ACHUN9_004384"
     tRNA            complement(833..908)
                     /locus_tag="ACHUN9_004384"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(873..875),aa:Ile,seq:cat)
     gene            1033..1539
                     /gene="mug"
                     /locus_tag="ACHUN9_004385"
     CDS             1033..1539
                     /gene="mug"
                     /locus_tag="ACHUN9_004385"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0697589.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          363 a          388 c          441 g          362 t
ORIGIN      
        1 cgaaggcgat aacaatgaat aactcccccc gctacccgca gcgcgttcgc aatgatctgc
       61 gcttccgtga actgactgtg ttacgcgttg agcgcatcag cgccggtttt cagcgcattg
      121 tcctcggcgg cgaggcgctg gacggtttta catcgcgcgg ctttgacgat cacagcaaac
      181 tcttctttcc tcaacctgac gctcactttg tgccgccaac ggtaacggaa gagggcatcg
      241 tctggccgga aggaccacgc ccaccgtcgc gtgactatac gccgctgtat gacgaactac
      301 gccatgaact ggcgatcgat ttctttattc acgacggtgg ggtcgccagc ggctgggcga
      361 tgcaggcgca accgggcgat aaacttacgg ttgcaggtcc gcgcggttcg ctggtggtgc
      421 cggaagatta cgcgtatcag ctgtatgtct gcgatgaatc cggaatgccc gcactgcgcc
      481 gccgcttgga aacgttgagc aaacttgccg ttaaaccgca agttagtgcg ctggttagcg
      541 tgcgggataa cgcctgtcag gattatctcg cgcaccttga tggttttaat atcgaatggc
      601 tggcacatga tgagcaggcg gtagatgcgc gtctggcgca gatgcaaatc cctgccgatg
      661 attacttcat ctggataacc ggcgaaggta aagtcgttaa gaatttaagc cgccgctttg
      721 aagcggaaca gtatgaccca cagcgggtac gtgcagcggc ttactggcac gcaaagtaac
      781 tgatatataa aaagaaaaag gctgacgatt tctcgtcagc ctttgctata tctggtggcc
      841 cctgctggac ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa ccactgagct
      901 aaggggccat ggtagcggat tataaagtaa ctccgtgtcg caatccagcc attaccgtgc
      961 gcctgctgtt tttataaaca atgtattttc aatccgttat acttttcctg gtgatgttag
     1021 aaaggagtaa atatggttga ggatattttg gctccagggt tacgggtcgt gttttgcggt
     1081 atcaaccctg ggctttcatc cgccgggact ggttttccct ttgctcatcc agcaaatcgc
     1141 ttctggaagg tgatatatca ggccgggttt accgaccgtc agttgaagcc gcaggaggca
     1201 cagcatctgc tggattatcg ttgtggcgtc accaaactgg tagaccgtcc aacggtgcaa
     1261 gccaatgaag tttcaaagca ggagctgcac gcaggtgggc gtaagctgat tgaaaaaatt
     1321 gaagattatc agccgcaggc gttggcgatt ctgggcaaac aagcatatga acagggattc
     1381 agccagcgcg gtgcacagtg ggggaaacaa acgctcacca ttggttcgac gcagatttgg
     1441 gtgctgccaa atcccagcgg tttaagtcgc gtttcactag agaaactggt tgaagcgtat
     1501 cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa aaacgccacc gaaa
//
LOCUS       DAZOYS010000386         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOYS010000386 DAZOYS010000000
VERSION     DAZOYS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.389"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHUN9_004386"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHUN9_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHUN9_004387"
     CDS             complement(397..954)
                     /locus_tag="ACHUN9_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0697590.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHUN9_004388"
     CDS             complement(951..1226)
                     /locus_tag="ACHUN9_004388"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0697591.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHUN9_004389"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHUN9_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZOYS010000387         1511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOYS010000387 DAZOYS010000000
VERSION     DAZOYS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.390"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..945)
                     /locus_tag="ACHUN9_004390"
     CDS             complement(<1..945)
                     /locus_tag="ACHUN9_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0697592.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAK"
     gene            complement(971..>1511)
                     /gene="rfaY"
                     /locus_tag="ACHUN9_004391"
     CDS             complement(971..>1511)
                     /gene="rfaY"
                     /locus_tag="ACHUN9_004391"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0697593.1"
                     /translation="TKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSA
                     GLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHA
                     LNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYY
                     SVIYRTKLRKFIKKLKGKA"
BASE COUNT          466 a          321 c          263 g          461 t
ORIGIN      
        1 ctttgcatgt tgatatttca cttgatactg cttttcattc gtggctggaa taaacgccac
       61 atcattccag caagaagcct gataagcctc attaaaatac ttctggcagg gataattcgt
      121 ccagtcgttc cagggcttcg ttacgccaac ataatgaatc agcttagtat catcggtaat
      181 ggtcttttta taatcttcat cgcttttatt ttttaactca taatcgatgc cataaaagca
      241 atcataatcg ccgctaataa aaatacaatg accaaagaac agaatattta aaatatcctg
      301 atcaaggcat ttatacttcg tgtgctgcgg gtcacttgcc agcatagcaa tggctttatt
      361 cataaaatca tttttcgccc atgcttcaag ctgcagatat actacaccgg cgttgaaata
      421 attccctgga agtccctcta tagccagacg cttcgctggt ttttcctgca taaaaggcac
      481 atctttaata acagctgcaa acttatcccc atcaaaatta atatcaagca atccagctaa
      541 tgagccttta cagatgacat cagcatcgat atataacaga ttttcaatag tttcactcag
      601 atactggaac gcaactaaac ggaagaatgt tgcataagac cactgtttca ggctggggaa
      661 gatatcaata aaggcactgg agactttata aattcttact tcaacgttag catttttgct
      721 ggtaaaagca ttaacacgtt gtagataatc ttcatcaatg tagtcggtaa agagatgaaa
      781 cttaaccgca aggtccggat tatgcataac aactgattgc atagatactg ccgcaccaaa
      841 taaaaagtta cggtcaatac cgtaagcgat atttaacaca cgatcggtgt tagcaatttc
      901 cccagagaca gcgacttcag taatactctc agctaataaa tccatgttgg ttccgttttg
      961 actgtgtggt ttacgctttg ccttttaatt ttttaataaa tttgcgtagt ttggttctgt
     1021 agattactga atagtagcca taatctttta tttcgttggc aatacccagg tgacgttcca
     1081 tggccagacg atcacgagct ttacgctctg ccgtacagct tttaccggat aggtcaatta
     1141 tcctgacacc atctttacta acgataaagt taccccggtg tgggtcgccc gatagcatat
     1201 ttaaggcatg caatttttcc attgaagcct taatttctgc tttaacatct tctggaatga
     1261 tcggcatatc attgagctct acgccctcga catactcgat aagcatgata aaaacactgg
     1321 catagttgaa aattttacgc tcagccagaa aataaaaatc attaggaaaa gtaagacctg
     1381 cacttctgac acgatcggtt tcaacgatca aattctgata gtagtcacct tttacaaaag
     1441 attttaaaaa acgttcattc cttttcgttt tcggggcaaa gactttaaaa acatagcgac
     1501 catatttcgt a
//
LOCUS       DAZOYS010000388         1493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOYS010000388 DAZOYS010000000
VERSION     DAZOYS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.391"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            34..477
                     /gene="cmtB"
                     /locus_tag="ACHUN9_004392"
     CDS             34..477
                     /gene="cmtB"
                     /locus_tag="ACHUN9_004392"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0697594.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            505..>1493
                     /gene="cmtA"
                     /locus_tag="ACHUN9_004393"
     CDS             505..>1493
                     /gene="cmtA"
                     /locus_tag="ACHUN9_004393"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0697595.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          347 a          330 c          383 g          433 t
ORIGIN      
        1 gacggtaccc taaaaataaa ataaggataa tttatgcggc ttagtgacta ttttccagaa
       61 tcatcaatct cagttataca ttcagcaaaa gattggcagg aagctatcga tttctcgatg
      121 gtatcattgc tggataaaaa ctatatcagc gagaattaca ttcaggcaat taaagattcc
      181 accattaaca atggccctta ttatattctc gcaccaggcg tggcaatgcc tcatgcgcga
      241 ccggaatgtg gggcgcttaa aaccgggatg tctttgacat tacttgaaca aggtgtttat
      301 tttccgggga atgacgaacc aataaaatta ctcatcggcc tttctgctgc cgatgccgat
      361 tcgcacattg gcgctattca ggcgttaagt gaattactgt gcgaagaaga aatactcgaa
      421 caactcttaa cagcagcatc agaaaaacaa ttagcggaca taatcagccg cggataatta
      481 ctctctcctc tacaggataa tattatggaa aacaagtctg ctcgtgcaaa ggtccaggct
      541 tttgggggct ttttgacggc aatggtcatc cccaatattg gtgcttttat tgcctggggt
      601 tttattactg cgttatttat tcccaccggt tggctgccta acgaacattt cgccaaaatt
      661 gtcggcccga tgattaccta tttattgccc gtgatgattg gttctaccgg tggtcatctg
      721 gtcggcggta aacgcggcgc ggtcatgggc ggaataggta ctattggtgt gatcgtcggc
      781 gcagagatcc cgatgttcct tggctcaatg attatggggc cgctcggtgg gctggtcata
      841 aaatatgttg ataaggcact ggaaaaacgc atacctgccg gttttgagat ggttatcaat
      901 aacttctcat taggtatcgc ggggatgctc ctttgtctgc tgggttttga agttatcggc
      961 ccggcggtgt taattgccaa tactctcgtc aaagagtgta ttgaggcgct ggtacatgcg
     1021 ggttatctgc ctctgctgtc agtcatcaat gaaccggcga aagtgctttt cctcaataat
     1081 gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac aggcttcggt taacggtaaa
     1141 tccatcttct ttatggtggc gtccaatcct gggcctggct tagggctgct gctggcgttt
     1201 accttgtttg gtaaagggat gagtaaacgt tctgcgcccg gggcgatgat tattcacttc
     1261 ctcggtggga tccacgaact gtatttcccg tatgtgctga tgaagccgct gaccattatt
     1321 gccatgattg cgggcggtat gtctggcacc tgggtgttta acttactgga cggtggtctg
     1381 gtggctggcc caagtccggg gtctatcttt gcttacctgg cactgacgcc gaaaggctcg
     1441 ttcctggcga caattgccgg tgttacggta gctaccctgg tgtcctttgc tat
//
LOCUS       DAZOYS010000389         1481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOYS010000389 DAZOYS010000000
VERSION     DAZOYS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.392"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="ACHUN9_004394"
     CDS             complement(<1..1011)
                     /gene="cadA"
                     /locus_tag="ACHUN9_004394"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0697596.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1091..1459)
                     /gene="cadB"
                     /locus_tag="ACHUN9_004395"
                     /pseudo
     CDS             complement(1091..1459)
                     /gene="cadB"
                     /locus_tag="ACHUN9_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          323 c          371 g          374 t
ORIGIN      
        1 ggtgtaaggc acccacgcgg agtcaaagtg gatggatttc acatccagtg ttttcttgat
       61 gaagtcggtg ttgtacagca gaccatcata ggtagagttg gtaattacag catgtaccgg
      121 ccaggttgcg tttggtgttt ctttcacgcg cttagcaatg gtagcgtgct ggaattcact
      181 ctgtgggata ccaccaagaa taccgtaagc gttacgggtc gggcggaaat agattggcgt
      241 aacatcgctc atcatcatca ggtgggtcag cgatttgtgg cagttacggt caatcagaat
      301 ggtgctgcct gccggagcag agtacatgcc aacaattttg ttcgcagtgg aagtaccgtt
      361 ggtcaccatg tagctgcggt ctgcgttaaa gacgcgagcg atatactgtt ctgcttcttt
      421 gtgtggacca ctatgatcca gcagagaacc cagttcagat actgaaatgg aaatatcaga
      481 tttcatggta ttcggaccaa agaaatcata gaacaggcta cctaccgggc ttttctggaa
      541 tgcagtaccg cccatgtgac caggagtaca gaaagtatat ttaccttcac gaacatattt
      601 aaacagtgct ttagtcagcg gaggcagaat agtgttgata tattcgtcag tggtctgctt
      661 gatcttatta gcaatatctt cagcagcacc cagcgcatat tcaaagaagc taatctgtaa
      721 acgcaggtca ttcaggctta catcgagagt ggaatacgta ttagcgaacg cgtacaacgg
      781 caggttctcg ttcattttgc taatttcttc gcacagctcg agattatatt tatcccagtc
      841 aaaaataacg ccgcacagac gcgcattgtt ttcgatcagt tttaataagt cgtcacggtc
      901 gttcgggtaa acaatctgga agttcagacg ttcaagcgcg cgatgaagtt cacggatggg
      961 ttcttcttta aaataaaccc ccatgtgatt caatattgca ataacgttca tagtcatatc
     1021 tccaggtaaa aaaggcccct cccaacacat gggacaaaat gaaaggagga gcctcggaaa
     1081 atatttttaa ttaatgtgcg ttagacgcgg tgtggttatc cattgagtgg ctctggcgct
     1141 cgtgcatttt gcgagcgtag aacatcagga taatcaggct gacgatgaag gtacctgcca
     1201 gctcgaagga gcttgcgccc atcagcgcga tgaagcagaa cacacaaccc agtacagagc
     1261 agatcaggct gacaaagttg cggatgttaa cgccttcaaa acgaatcagg tcaacgcaag
     1321 agtagaagta cggcagcata gtcagcagta ctgcgatacc ggtcagttca ccgaacaggt
     1381 cagatgcttt accaccggca gagttcatca gagtgatgag gatcatcagg gcagtcattt
     1441 tcactgcagc cagcagcagt aatgccgatc agttaaggat c
//
LOCUS       DAZOYS010000390         1474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOYS010000390 DAZOYS010000000
VERSION     DAZOYS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.393"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1292
                     /locus_tag="ACHUN9_004396"
     CDS             <1..1292
                     /locus_tag="ACHUN9_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0697597.1"
                     /translation="YHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRER
                     DLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1270..>1474
                     /locus_tag="ACHUN9_004397"
     CDS             1270..>1474
                     /locus_tag="ACHUN9_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697598.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          394 a          313 c          462 g          305 t
ORIGIN      
        1 actatcacta cgacagccag caccgcctgg tgttctacac gcggatacag cacggcgaac
       61 cacaggtgga gagccggtac ctctatgacc cgctgggccg ccggacggga aaacgggtat
      121 ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc gcgtaaaccg gaggagacct
      181 ggtacgggtg ggacggtgac aggctgacca ctgtacagac ccaacagaca agaatccaga
      241 cggtatacca gccgggaagc ttcacgccgc tcctgagaat cgaaacagag aatggcgaac
      301 aggcgaaggc gcggcaccgg agcctggcgg aggtgttgca ggaggacacg ggggtgacgc
      361 ttccggcgga gctggcggtg atgctgggga ggctggagcg ggaactgcgt gcgggtgcgg
      421 tgagtgcgga gagtgaggca tggcttgcgc agtgcgggct gacggtggag cagatggaaa
      481 gccagatgga ggcggaatac atcccggagc ggaggctgca tctttaccac tgtgaccacc
      541 ggggactgcc gcaggcgctc atcagcccgg aaggggaaac ggcgtggcgc ggggagtatg
      601 acgagtgggg gaaccagctt aatgaggaga acccgcatca cctgtaccag ccgtaccgtt
      661 tgccggggca gcagtatgat gaggagtcgg gactgtatta caaccgtcac cggtactacg
      721 atccgttgca ggggcggtat atcacccagg atccgatagg gttgaagggg ggaataaatc
      781 tatacacgta tccactggca ccaatacgtt acactgatcc actggggtta gaacgagtta
      841 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      901 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      961 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
     1021 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
     1081 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
     1141 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1201 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1261 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1321 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1381 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1441 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZOYS010000391         1470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOYS010000391 DAZOYS010000000
VERSION     DAZOYS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.394"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            353..1141
                     /gene="phoH"
                     /locus_tag="ACHUN9_004398"
     CDS             353..1141
                     /gene="phoH"
                     /locus_tag="ACHUN9_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0697599.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT          354 a          347 c          394 g          375 t
ORIGIN      
        1 atgcgagatc tgttgtactt attaagtagc agcggaagtt cccggcagtg atagtcagtc
       61 actatggaga tcgcggatgg taacgtcctg tactggacat gttttagata atcaacgcgc
      121 caccactcgc ggagttttct cctccggtag tcatctcgtc actttgcatt ttcaacctca
      181 tcctttcttt tcatgtgtta ccgacgccgt aaacggtgcg cgtagccgtt tttcggcatt
      241 ttatccaaaa gcaaactatg gcttacaagg aagccaaccc tctgatgttc gtgcgcataa
      301 tcgcgctgcc aacggcgcgt gtgatgaata caaacaactc aaggtgctct ctatgggaag
      361 acaaaaagca gtgatcaaag ctcgtcgcga ggcaaaacgt gtgctgagac gggattcgcg
      421 cagccataag cagcgtgaag aagaatcggt cacctcgctt gtgcagatgg gcggcgtaga
      481 agccattggt atggcccgcg acagtcgcga tacttcgccc atcctcgcgc gaaatgaagc
      541 gcaattgcac tatctgaagg ctattgagag taagcagctg atattcgcca cgggcgaagc
      601 cgggggcgga aaaacctgga tcagcgcagc aaaagcggca gaggccctga tacataagga
      661 tgtcgacagg attatcgtca cccgtccagt tctgcaagcc gatgaagatc ttggcttctt
      721 acctggagat atcgcagaaa agtttgctcc ctattttcgc ccggtctacg acgtgctggt
      781 ccggcgctta ggggcttcct ttatgcagta ctgcctgcga ccggaaatag ggaaggtgga
      841 aattgcgccg ttcgcctata tgcgtggacg tacctttgaa aatgcagtcg tcattcttga
      901 cgaggcgcag aatgtgactg ccgcgcaaat gaaaatgttt ttaacccgcc tcggggagaa
      961 cgtgacggtt atcgtcaacg gtgatatcac gcaatgcgat ttgcctcgcg gcgtgtgctc
     1021 cggattaagt gacgcgctgg aacgtttcga agaagatgaa atggttggga tcgtccggtt
     1081 cggtaaagag gactgcgtac gttcggcact ttgccaacgt acgctgcatg cctacagtta
     1141 agtgtgttat cggtgcagag cccgggcgaa ccgggctttg ttttatttca acagattttc
     1201 acttcaggtt agcgttcagg ggggcgactt tgagcgtgtt gataagctga actggagtct
     1261 ggtttagata ctcttaccag gcatttttcg tcatagcatc agaccagtgt aatactgcca
     1321 gcgcggagag tttttcttcc agaagccaaa aaaatgagca tctgcctatc ttttttcggc
     1381 cattcaacct gtacaggaga tctggtgcca attttgctta tgaggtttaa tcaacaatgg
     1441 gctatacttt gactgactgt ttaacaccga
//
LOCUS       DAZOYS010000392         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOYS010000392 DAZOYS010000000
VERSION     DAZOYS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.395"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUN9_004399"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUN9_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0697600.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUN9_004400"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUN9_004400"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0697601.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHUN9_004401"
     CDS             complement(822..>1426)
                     /locus_tag="ACHUN9_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0697602.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZOYS010000393         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOYS010000393 DAZOYS010000000
VERSION     DAZOYS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.396"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUN9_004402"
     CDS             <1..459
                     /locus_tag="ACHUN9_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0697603.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUN9_004403"
     CDS             477..1115
                     /locus_tag="ACHUN9_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0697604.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZOYS010000394         1391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOYS010000394 DAZOYS010000000
VERSION     DAZOYS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.397"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..469
                     /locus_tag="ACHUN9_004404"
     CDS             <1..469
                     /locus_tag="ACHUN9_004404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697605.1"
                     /translation="LFAGQIGYNAYDKWKESSDDVFKMRVILLSEINNNLLALATGGD
                     ESQVLGTYEICIPLPTEDTEAIKRVATAVSYLHDDVYYSQLEKLASLPKKEVDAIVKS
                     YYSLSRLRTLSTQLAYNIKYSPPKVKLLREEYYNLYTLSSSLSQYLSYHTENP"
     gene            complement(480..>1391)
                     /locus_tag="ACHUN9_004405"
     CDS             complement(480..>1391)
                     /locus_tag="ACHUN9_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697606.1"
                     /translation="PDEMLKNATAYGAKQRTDLLNSMVGYQDPNQGLKDTNALIGSGL
                     LNDNQKGYLAQQQNQRINGGNPLAYNDTDQKLKDNADAMNAELQQNELLLKGHEDYEK
                     RKAEITAKYNAQAIQISNQNAQDQLSIFSSTAQSLSQGMVDAFGESSGAAQAAFTLSK
                     SISIAQTVLSIQSALAQALATPWPASLANYAQVLSLGMSIISTAKGAAAGQFHGGVDE
                     LPASYDNKSFVLKQGERVVQPEANKKLTAFLDKQEGGSTSGDITVNAPLIIQGDVADD
                     DKKFNEMLKKHANSVSQAVRSSQKRNT"
BASE COUNT          385 a          302 c          256 g          448 t
ORIGIN      
        1 gttatttgca ggccaaatag gctataacgc atatgataaa tggaaagaaa gttcagatga
       61 tgtttttaag atgcgtgtaa tcttattgag cgaaatcaac aataaccttt tagctcttgc
      121 tactggtggc gatgagtctc aggtgttagg cacatatgaa atatgtattc ctcttccaac
      181 tgaagataca gaagctatta aaagagtagc aactgccgtt tcttatttgc atgatgatgt
      241 atattattcc caattagaaa aattagcatc actcccaaaa aaagaagtag atgccatcgt
      301 aaagagttat tattcattat cgagattacg tactctttca acacagcttg catacaatat
      361 taaatactca ccacctaaag ttaaattatt gcgggaagag tattacaatc tctataccct
      421 ttcatcttct ctttcacaat atttgtctta tcacacggaa aatccctaac tgggctttct
      481 tatgtattac gtttttgact acttctcaca gcttgagaaa cactattggc gtgtttctta
      541 agcatctcat taaatttctt gtcatcatca gctacatcac cctggatgat caatggtgcg
      601 ttaacggtaa tatcacctga agtacttcca ccttcttgtt tatccaagaa tgctgttaat
      661 ttcttattcg cttcaggctg caccactctc tcgccctgct taagtacaaa tgatttgttg
      721 tcataacttg ctggtagttc atctaccccg ccatggaact gaccagcagc agcacccttt
      781 gcagtactaa taatcgacat accaagtgaa agtacttgtg catagtttgc caatgaagct
      841 ggccaagggg tagcgagtgc ttgggccaat gctgactgaa ttgaaagtac tgtctgtgca
      901 atgcttatgc tcttacttag ggtaaatgca gcttgtgcag ccccactact ttccccaaac
      961 gcatcaacca tgccctgtga tagtgactgt gcagtacttg agaagatact taactgatcc
     1021 tgtgcattct ggttactgat ttggatggcc tgtgcattgt actttgccgt gatctcagct
     1081 ttacgtttct catagtcctc atggcctttc agcaacaact cattctgctg aagttcagca
     1141 ttcatagcat cagcgttatc tttcaacttc tgatcggtat cgttataggc taatggatta
     1201 ccaccattga tacgctgatt ctgttgctgt gccagatacc ccttctgatt gtcattgagt
     1261 agaccggaac caataagagc attggtatct ttcagaccct gattaggatc ttgataacca
     1321 accattgaat tcagtaaatc agtacgttgt ttagcaccat atgcagtagc gttctttagc
     1381 atttcatcag g
//
LOCUS       DAZOYS010000395         1388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOYS010000395 DAZOYS010000000
VERSION     DAZOYS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.398"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..617)
                     /locus_tag="ACHUN9_004406"
                     /pseudo
     CDS             complement(1..617)
                     /locus_tag="ACHUN9_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            complement(781..876)
                     /gene="ymiC"
                     /locus_tag="ACHUN9_004407"
     CDS             complement(781..876)
                     /gene="ymiC"
                     /locus_tag="ACHUN9_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0697607.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(990..1157)
                     /gene="yciX"
                     /locus_tag="ACHUN9_004408"
     CDS             complement(990..1157)
                     /gene="yciX"
                     /locus_tag="ACHUN9_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0697608.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1160..1300)
                     /locus_tag="ACHUN9_004409"
     CDS             complement(1160..1300)
                     /locus_tag="ACHUN9_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0697609.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          374 a          336 c          323 g          355 t
ORIGIN      
        1 gccgttgatc atagtggtgt gcgagtcagt accaacgagt gtatccggat aagcaatcca
       61 ttcaccgtcc tgcaattcac tccacactgc tttgccgaga tattcgaggt taacctgatg
      121 gcaaatgcct gtgcctggcg gcacgacgct aaaccgactg aacgcttgct ttccccattt
      181 caggaacaca taacgttcgt ggttgcgctc catttccagg cgtacgtttt cttcaaatgc
      241 ctcatcatca ccaaaacgat cgacggtcac cgagtggtca atgaccaggt cgaccggtga
      301 gagcgggtta acctttgcag tatcgccgcc gaggcgttta accgcttcgc gcattgccgc
      361 cagatcaaca acggcaggta cgccggtaaa gtcctgcatc aacacccttg ccgggcggta
      421 ggcaatttca cggtcagcat gggcattttt cagccatcct gccagcgcag caggttttcg
      481 agcaaaactt tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc
      541 ggcaggctgt agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct
      601 cgtagggttg acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc
      661 agggtgaata ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga
      721 taaaagagga gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga
      781 tcagtgagta atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc
      841 catccaggac caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta
      901 ttttgtgttg agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca
      961 ttaaaaatgg gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac
     1021 agttctataa aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa
     1081 cattcccgtt tcgtttctgt ttcattgtca ctatgctggc ataatggtga cgactccagt
     1141 tgctcctgac cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc
     1201 agaaaagtgc agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcgc
     1261 ggcggggttc ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca
     1321 gcaagacgtt gaacgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt
     1381 tagtcaaa
//
LOCUS       DAZOYS010000396         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOYS010000396 DAZOYS010000000
VERSION     DAZOYS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.399"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHUN9_004410"
     CDS             complement(<1..1339)
                     /locus_tag="ACHUN9_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0697610.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZOYS010000397         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOYS010000397 DAZOYS010000000
VERSION     DAZOYS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.400"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHUN9_004411"
     CDS             <1..984
                     /locus_tag="ACHUN9_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0697611.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHUN9_004412"
     CDS             999..>1377
                     /locus_tag="ACHUN9_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0697612.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          361 a          315 c          361 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacac gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZOYS010000398         1370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOYS010000398 DAZOYS010000000
VERSION     DAZOYS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.401"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            197..1279
                     /locus_tag="ACHUN9_004413"
     CDS             197..1279
                     /locus_tag="ACHUN9_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0697613.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          410 a          283 c          294 g          383 t
ORIGIN      
        1 ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag aaaaaagttc
       61 agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta catcttgaaa
      121 taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta ataatagttc
      181 aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt
      241 actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta acaagctgga
      301 tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag atgatggtga
      361 tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc aactgactgg
      421 tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag gttcctccaa
      481 agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta
      541 cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc tgccagaatt
      601 cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca ctggtgttgc
      661 aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta
      721 tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt tcggtttctc
      781 cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac
      841 taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt
      901 atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga
      961 aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga
     1021 agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc
     1081 taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata ttgatgtagg
     1141 tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat
     1201 tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg
     1261 tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc tttgcctccc
     1321 caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
//
LOCUS       DAZOYS010000399         1364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOYS010000399 DAZOYS010000000
VERSION     DAZOYS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.402"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(261..>1364)
                     /locus_tag="ACHUN9_004414"
     CDS             complement(261..>1364)
                     /locus_tag="ACHUN9_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0697614.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          352 a          372 c          318 g          322 t
ORIGIN      
        1 aatagatgga aaagcactcc tgctcttttt tgattagagc cgtcgcagga ggaacaactc
       61 atatcatcat tgcgatctcg acgatgtata gtctcatcct gcggcagaac aagacggata
      121 aaaaatccaa tgagagcaca ctcatccaga acttattaac ataatgaata ttaaagaaaa
      181 atcatcgcag ccaatgaagt aatatgcaaa aataattagc ccttgcaggg agcaaagttc
      241 cataatctcc cccttccccc tcaatgatcc aaatacaaat aatgcatcca actggtcatt
      301 cttagcagca ccttccgcat caccgaaaca tggctgaaat ggtcggagta aactgctacc
      361 tgggcgtaaa tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt
      421 cccagcacgc cgtccgtgcc gggaacgacc gttaatgatt gcaatacccc ctgcgcctga
      481 taagaaccgc ctggcacgac aggtaaaata ctagtcaatt tgccgtggaa cacctgccca
      541 ggtagcgcgt taaacaccac ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt
      601 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc
      661 agcggcaagg cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg
      721 ctcggcgcgc gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt
      781 aagctcacaa tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc
      841 gcctgctccg ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca
      901 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt caggtaacgt
      961 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct
     1021 tcagtgagct gcgcacgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga
     1081 agtctgtcaa ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt
     1141 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     1201 atagggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     1261 aacaaaatca aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac
     1321 tgattcaccg gaatacgaaa gattttaaag accgcccacg ccag
//
LOCUS       DAZOYS010000400         1364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOYS010000400 DAZOYS010000000
VERSION     DAZOYS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.403"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHUN9_004415"
     CDS             complement(<1..1259)
                     /locus_tag="ACHUN9_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0697615.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQSSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          304 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaagattgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAZOYS010000401         1363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOYS010000401 DAZOYS010000000
VERSION     DAZOYS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.404"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            404..859
                     /gene="csgB"
                     /locus_tag="ACHUN9_004416"
     CDS             404..859
                     /gene="csgB"
                     /locus_tag="ACHUN9_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0697616.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            900..1352
                     /gene="csgA"
                     /locus_tag="ACHUN9_004417"
     CDS             900..1352
                     /gene="csgA"
                     /locus_tag="ACHUN9_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0697617.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          404 a          267 c          325 g          367 t
ORIGIN      
        1 gtgtagtcct ttcgtcatgt aaaaagttgt tgttttttct ccacacctcc gtggacaaat
       61 ttttactcca aaaagacgag gtttgtcacg gcttgtgcgc aagacatatc gcagcaatca
      121 gcgatgggca agaagaatga ctgtctggtg atttttgata gcggaaacgg agatttaaaa
      181 gaaaacaaaa tattttttgc gtagataaca gcgtatttac gtggctttta atactttggt
      241 atgaacaaaa aaagaaaaat acaacgcgcg ggtgagttat taaaaatatt tccgcagaca
      301 tactttccat cgtaacgcag cgttaacaaa atacaggttg cgttaacaac caagttgaaa
      361 tgatttaatt tcttaaatgt acgaccaggt ccagggtgac aacatgaaaa acaaattgtt
      421 atttatgatg ttaacaatac tgggtgcgcc tgggattgca gccgcagcag gttatgattt
      481 agctaattca gaatataact tcgcggtaaa tgaattgagt aagtcttcat ttaatcaggc
      541 agccataatt ggtcaagctg ggactaataa tagtactcag ttacggcagg gaggctcaaa
      601 acttttggcg gttgttgcgc aagaaggtag tagcaaccgg gcaaagattg accagacagg
      661 agattataac cttgcatata ttgatcaggc gggcagtgcc aacgatgcca gtatttcgca
      721 aggtgcttat ggtaatactg cgatgattat ccagaaaggt tctggtaata aagcaaatat
      781 tacacagtat ggtactcaaa aaacggcaat tgtagtgcag agacagtcgc aaatggctat
      841 tcgcgtgaca caacgttaat ttccattcga cttttaaatc aatccgatgg gggttttaca
      901 tgaaactttt aaaagtagca gcaattgcag caatcgtatt ctccggtagc gctctggcag
      961 gtgttgttcc tcagtacggc ggcggtaacc atggtggtgg cggtaataat agcggcccaa
     1021 attctgagct gaacatttac cagtacggtg gcggtaactc tgcacttgct ctgcaaactg
     1081 atgcccgtaa ctctgacttg actattaccc agcatggcgg cggtaatggt gcagatgttg
     1141 gtcagggctc ggatgacagc tcaatcgatc tgacccaacg tggcttcggt aacagcgcta
     1201 ctcttgatca gtggaacggc aaaaattctg aaatgacggt taaacagttc ggtggtggca
     1261 acggtgctgc agttgaccag actgcatcta actcctccgt caacgtgact caggttggct
     1321 ttggtaacaa cgcgaccgct catcagtact aatacatcat ttg
//
LOCUS       DAZOYS010000402         1358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOYS010000402 DAZOYS010000000
VERSION     DAZOYS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.405"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            218..>1358
                     /locus_tag="ACHUN9_004418"
     CDS             join(218..479,479..>1358)
                     /locus_tag="ACHUN9_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
                     /protein_id="HFR0697618.1"
                     /translation="MTKTVSTSKKTRKQHSPEFRSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILPKGRDILCEAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQFRQHCDSVVLAAAFTRS
                     KQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENL
                     LEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRLTAQLACDA
                     LQMALWRRKRPRNVIVHSDRGSQYCSADYQALLKWHNLRGSMSAKGCCYDNACVESFF
                     HSLKVECLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPE"
BASE COUNT          326 a          357 c          388 g          287 t
ORIGIN      
        1 tgcatgttcg tcaccgctgt cggaacctgc gagtcgagtc agccggagag agtttcactc
       61 tccagctgct cacggaaccg tacgtgacac tctcgcgtca tacggctctt gatcactcat
      121 tcgatgcacg tagtaaatac cagtgagcaa attgatctta cccagcaata gtggacacgc
      181 ggctaagtga gtaaactctc agtcagaggt gactcacatg acaaaaacag tatcaaccag
      241 caaaaaaaca cgtaaacagc attcgcctga atttcgcagt gaagccctga agcttgctga
      301 acgcatcggt gttgctgccg cagcccgtga actcagcctg tatgaatctc agctctatgc
      361 gtggcgcagc aaactgcagc aacagatgac ctcgtctgag cgtgaaagtg aactggccgc
      421 tgaaaatgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg ctatcctcca
      481 aaaggccgcg acatactttg cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat
      541 caggctgagt tcagcatcaa ggcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg
      601 tatacgtggt gtcagcggcg gacagggata agccctcgtc agcagttccg tcagcactgc
      661 gacagcgttg tcctcgcggc ggcctttacc cggtcaaaac agcgttacgg tgcaccacgc
      721 ctgacggatg aactgcgtgc tcagggttac cactttaacg taaaaaccgt ggcggcaagc
      781 ctgcgccgtc aggggctgag ggcaaaggcc tcccggaagt tcagcccggt cagttaccgc
      841 gcacatggcc tgcctgtgtc agaaaatctg ctggagcagg atttttacgc cagtggtccg
      901 aaccagaagt gggcgggaga catcacgtac ttacgtacag atgaaggctg gctgtatctg
      961 gctgtggtca ttgacctgtg gtcacgtgcc gttattggct ggtccatgtc accacgcctg
     1021 acggcgcaac tggcctgcga tgccctgcag atggcgctgt ggcggcgtaa gagaccccgg
     1081 aacgttatcg ttcactcgga ccgaggaagt cagtactgtt cagcagatta tcaggcgctg
     1141 ctgaagtggc ataatctgcg tggcagtatg agcgcaaagg gctgctgcta cgataatgcc
     1201 tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgtc tccacggaga acactttatc
     1261 agccgggaaa taatgcgggc aacagtgttt aattatatcg aatgtgatta caatcgctgg
     1321 cgacggcaca gttggtgtgg cggcctcagt ccggaaca
//
LOCUS       DAZOYS010000403         1353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOYS010000403 DAZOYS010000000
VERSION     DAZOYS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.406"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..1353
                     /gene="rhsB"
                     /locus_tag="ACHUN9_004419"
                     /pseudo
     CDS             1..1353
                     /gene="rhsB"
                     /locus_tag="ACHUN9_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          334 a          388 c          420 g          211 t
ORIGIN      
        1 actggcggtg gtgtatgacc gtagtggcaa acaggtgcgc agctttactt acgatgataa
       61 ataccggggc cggatggtgg cgcaccgtca cacgggccgg ccggaaatcc gttaccgtta
      121 cgacagcgac gggcgggtga cagaacagct taacccggca ggcttaagct acacgtatca
      181 gtatgagaag gaccgcatca ccatcaccga cagcctgaac cgccgtgaag tgctgcacac
      241 gtagggtgaa gccgggctga agcgggtggt gaaaaaggaa cacgcggacg gcagcgtcac
      301 gcagagtcag tttgacgccg tgggcaggct caggacacag acggatgccg caggccggac
      361 aacagaatac agcccggatg tggtgacggg cctcatcacg cgcatcacca cgccggatgg
      421 cagggcatcg gcgttttact ataaccacca cagccagtta acgtcagcca ccgggcctga
      481 cgggctggaa atgcgccgca aatatgatga atccggccgt ctgattcagg aaaccgcccc
      541 tgacggcgat atcacccgct accgttatga taatccacac agtgacttac cctgcgcaac
      601 ggaagatgcc accggcagcc ggaaaaccat gacatggagc cgttacggtc agttgctgac
      661 cgtcactgac tgttccggtt atgtaacccg ttatgaccat gaccgttttg gtcaggtgac
      721 ggcggtgcac cgcgaggaag ggctgagcca gtaccgcgca tacgacagcc gtggacagtt
      781 aattgcggtg aaagacacgc agggccatga aatgcggtat gaatacaacg ccgccggtga
      841 cctgaccgcc gtcattgccc cggacggcag cagaaacggg acacagtacg atgcgtgggg
      901 aaaagccatc tgtaccacgc agggcgggct gacgcgcagt atggaatacg atgctgccgg
      961 acgggtcatc cgtctgacca gtgaaaacgg cagccacacc accttccgtt acgatgtact
     1021 cgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc accacgacct
     1081 gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccactggc actatgacga
     1141 agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt ggcggtacga
     1201 cgaacgcggc tggctgacag acatcagcca tctcagcgaa gggcaccggg tgacggtgca
     1261 ttacgggtat gatgagaaag gccggctgac cggtgagcgt cagacggtgc atcacccgca
     1321 gacggaagca ctgctctggc agcatgagac cag
//
LOCUS       DAZOYS010000404         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOYS010000404 DAZOYS010000000
VERSION     DAZOYS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.407"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUN9_004420"
     CDS             <1..184
                     /locus_tag="ACHUN9_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697619.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUN9_004421"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUN9_004421"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0697620.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZOYS010000405         1322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOYS010000405 DAZOYS010000000
VERSION     DAZOYS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.408"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUN9_004422"
     CDS             complement(<1..186)
                     /locus_tag="ACHUN9_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0697621.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(231..1217)
                     /locus_tag="ACHUN9_004423"
     CDS             complement(231..1217)
                     /locus_tag="ACHUN9_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0697622.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          357 a          294 c          271 g          400 t
ORIGIN      
        1 agcacctaat aaccctaccc agatcatcaa aaatcgtgcc agctcatcgg ttaatgtact
       61 tggctggtta agtacgtagc ggctaaatac ttgccagact acgcatacaa ccagagcaag
      121 catgacggcg acagaaaacg cggcaatgac gcggtctacc gcaagtttca ggcggttaaa
      181 tatcattgta agcatcttgg aaaatcaggg ccagagactg gcccggaata ttattgagca
      241 gcgttgtcga acttctctaa caaagcagct tgttttggat cttttttgaa gtcgtcaaac
      301 agaggctgaa ctgcggcacg gaatggtgct ttatccactt ttacaatttc accccccata
      361 gctttcgcct gagcacgtgt gtcagcgtca attttttccc acaatttttg ttgataagct
      421 tcggatttct tcgctgctgt ttcgaggatt tgtttctgtt ctggggttaa tttattccat
      481 gttttggtgg agataaccaa aaagtcagga attgaggcgt gttcatcttc agaaaaaact
      541 ttggcaattt caatatgtcg agtctgcacc caggaaggca cgttattttc tgcgccatct
      601 actacacctt gctgcatcgc agtataaact tcgccaaatg aaattggagt gggtgaacca
      661 cccatcagtt caatcatttt gatggtggtc ggacttggct gaacacgaat tttcagccct
      721 ttaagatctt ctggcttagt aatcggtttt ttcgcataga aactgcgtgt acccgctaca
      781 taagctgata aagcaaaaaa gcctttgtct tttgttgagt ccataatttc tttaccaact
      841 tcaccaaaaa caactttgtt gaagtgagcc tgatctttaa ataagaacgg caaattatag
      901 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
      961 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1021 atacggatca ccatattacc ttctgacagc tgttttacct ctttcgccag ctcttcaaaa
     1081 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgt cactttttct
     1141 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1201 aatgacctca ttttcataaa aaaactccct gttgaaggat ggaaattggt tgatatctaa
     1261 ttgtgcttat cagtgctttg tgcgacaaat tggttaacct gtctgacaac ttgtctatgt
     1321 tt
//
LOCUS       DAZOYS010000406         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOYS010000406 DAZOYS010000000
VERSION     DAZOYS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.409"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHUN9_004424"
     CDS             complement(<1..346)
                     /locus_tag="ACHUN9_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697623.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHUN9_004425"
     CDS             complement(349..1059)
                     /locus_tag="ACHUN9_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697624.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHUN9_004426"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHUN9_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0697625.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZOYS010000407         1317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOYS010000407 DAZOYS010000000
VERSION     DAZOYS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.410"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..245)
                     /gene="dinJ"
                     /locus_tag="ACHUN9_004427"
     CDS             complement(<1..245)
                     /gene="dinJ"
                     /locus_tag="ACHUN9_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0697626.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLF"
     gene            461..1204
                     /locus_tag="ACHUN9_004428"
     CDS             461..1204
                     /locus_tag="ACHUN9_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0697627.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          317 a          324 c          334 g          342 t
ORIGIN      
        1 tcaaataaat catcagcgtc tttggcctta tgaacatcaa cgccagcttc gctgtttttg
       61 attgattgaa tggttaattg attaggctcg cgtaaatcaa acggcaatgc cttttcacgc
      121 gcgacctttg tgagggttat gcgaaccagg tcagagatgg tcagccccat cccggccagt
      181 acgtccgccg cctgattctt cagatcttca tcgattcggg cgcgaacaaa cgcgttagca
      241 gccattgggg gatctcctgt gctgtatatt tattcagctt gaattgtagc tcaaatgagc
      301 aacagcgaca atccatgagt aatttaataa acggatgttt ttacataata aactgaaata
      361 tatttattaa ttttataata atggtttatt aagtttgttc aggtacgggc caggatacac
      421 tgcaacatca atgcatgacc agccattatt gagaatgtct ttgccgtcga ttccctcttt
      481 tgtgttgtcg ggattactgt tgctttgctt gccgttttct tcatttgcct gcgccaccac
      541 atcacatatc tctttcagct acgccgcccg ccagcggatg caaaaccgtg cgcgtttatt
      601 aaaacagtac caaacccatc tgaaaaaaca ggccagctac attgtggaag gcaatgccca
      661 aagcagaagg gcgctgcgcc agcacaaccg ggagcagata aaacagcatc cagaatggtt
      721 tcctgcgccg ctcaaggcga gtgacagacg ctggcaggcg ctggcggaaa acaaccactt
      781 tttaagcagc gatcacctgc ataacatcac cgaagtggcg attcaccgtc tggagcagca
      841 gcttggcaag ccttacgtct ggggcggcac gcggcccgat caaggcttcg attgtagtgg
      901 gttagttttt tatgcctaca acaagatcct cgaggcgaag ctcccgcgta cggccaatga
      961 gatgtatcac taccgtcggg caaccattgt ggcgaataac gacctgcgcc ggggagattt
     1021 gctgtttttc catatccaca gccgcgagat tgccgaccat atgggggtgt atctgggcga
     1081 cggtcagttt attgagtcac cgcgcaccgg ggaaaccatt cgggtaagtc ggttagctga
     1141 acctttctgg caggatcatt ttttgggcgc gcggcggata ttgacggaag agacgatttt
     1201 gtaggacgga taaggcgttt acgccgcatc cggcggtggt gcgcagatgc ctgatgcgac
     1261 gctgacgcgt cttatcatgc ctacaaatct gtgcgttttt gcgagccgct ttcctgt
//
LOCUS       DAZOYS010000408         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOYS010000408 DAZOYS010000000
VERSION     DAZOYS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.411"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHUN9_004429"
     CDS             132..1151
                     /locus_tag="ACHUN9_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0697628.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAZOYS010000409         1259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOYS010000409 DAZOYS010000000
VERSION     DAZOYS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.412"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..786
                     /locus_tag="ACHUN9_004430"
     CDS             <1..786
                     /locus_tag="ACHUN9_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697629.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            799..>1259
                     /locus_tag="ACHUN9_004431"
     CDS             799..>1259
                     /locus_tag="ACHUN9_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0697630.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSS"
BASE COUNT          403 a          250 c          241 g          365 t
ORIGIN      
        1 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
      781 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
      841 attgtgcaaa gtgctccctg tgcattgacc aatgtcgggg aacaacgtgg aacatatata
      901 ctcaagccac tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa caccggaaat
      961 atcattgagt gtgcagactt acaagagacg accaacgcca ttaaagttca ttttttaaca
     1021 cagaacgctt taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa
     1081 tctgacggta aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagttcatg
//
LOCUS       DAZOYS010000410         1249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOYS010000410 DAZOYS010000000
VERSION     DAZOYS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.413"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..1168
                     /locus_tag="ACHUN9_004432"
     CDS             <1..1168
                     /locus_tag="ACHUN9_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0697631.1"
                     /translation="RLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYNC
                     DKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPV
                     FESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGCL
                     NKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVLKA
                     GGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGET
                     ATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAK
                     VCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          296 a          307 c          402 g          244 t
ORIGIN      
        1 acgcctgttc acagccaacc agtgctggac gtccttcctg gatgcgggcg gtctgcgcga
       61 tatcgaggtt gaggctgtca caaaaatgct ggcctgcggc acgcggatac tgggagtgaa
      121 ggaatacaac tgcgataaac ctgagtgccc ccacgtcaga tacgtcacta actcatgcgg
      181 cagccgtgcc tgcccgtcct gcggaaaaaa ggccacagac ctgtggatag cgacacagct
      241 gaatcgtctt cctgactgcg actgggtaca tctggtcttc accctgccgg acacgctgtg
      301 gccggtgttc gaaagcaacc ggtggctgct gaatgacgtg tgccgtctgg cggtggagaa
      361 tctgctgtat gccgcccgga aacgggggca ggaacccggt atcttctgcg ccatccacac
      421 gtatggccgt cgtctcaact ggcatccgca tgtacatgtg tctgtaacct gtggatgtct
      481 gaataagcat ggtcagtgga aaaagctgag cttcctgaaa gacgcgatgc gttcgcggtg
      541 gatgtggaat atgcggcaac ggcttctgaa agcgtggtca gaggggctgg cgatgccgga
      601 gtcgttgtca catatcacga cggaatcaca gtggagaagc ctggtgctga aagccggagg
      661 aaaatactgg catgtgtaca tgtcgaaaaa aacggccggc gggaggaata cggcgcgcta
      721 cctgggtcgt tatctgaaga agccgccgat agcggcctcc cggctggcac attacaacgg
      781 aggggcgagc ctgagcttcc gttacctgga ccacaaaacg ggagaaacgg cgacggaaac
      841 gctgacacag cgtgagctgg tcgcgaggct gaaacagcac atcccggaga agttttttaa
      901 gatggtgagg tacttcgggt tccttgccaa ccgggtgtgt ggagagaagc tgccgcaggt
      961 gtaccgtgct ctggggatgg ataaaccgga accagtggcg aaagtgtgct atgcacaaat
     1021 ggtgaaacag ttcctgagtc gtgacccgtt cgaatgcgtg ctgtgtggcg gccggatggt
     1081 ataccgccgg gccattgcgg ggctgaatgt ggaggggctg aagaaaaacg cgcgggatat
     1141 cagtctgctg aggtatatgc cggcctgaca caggctaagt gtgcccggga atcggtgcaa
     1201 gtataaaaaa cagcctgtta ctgtataaaa accgaccata cagagagag
//
LOCUS       DAZOYS010000411         1234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOYS010000411 DAZOYS010000000
VERSION     DAZOYS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.414"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..761)
                     /locus_tag="ACHUN9_004433"
     CDS             complement(<1..761)
                     /locus_tag="ACHUN9_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0697632.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          307 a          299 c          310 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAZOYS010000412         1226 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOYS010000412 DAZOYS010000000
VERSION     DAZOYS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.415"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            79..>1226
                     /locus_tag="ACHUN9_004434"
     CDS             79..>1226
                     /locus_tag="ACHUN9_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR0697633.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGP"
BASE COUNT          399 a          235 c          241 g          351 t
ORIGIN      
        1 ggtaagaatc atgctttgat aaccaattga taactattat tatattaatc taacaccatt
       61 gacatagttg aggtttgaat gaacaaaaag ctgtatttat tcccagactc aaacattttt
      121 ctacaatgca aggacttgac gcaggtaaat ttttccgaga ttactgcttg tgatgaagta
      181 tgtatcatta ttacaaggcc cattcagcaa gagattgacc gccagaaagg gcaaggtaat
      241 tcccgcctat ccaaaaaagc aaggaaggct gctagcctat ttaatcaagt agttgattca
      301 actgacatga ctcttgttat tcgtgaacgc tcgccgcgag tttttcttac catggatcta
      361 agcctgaaac catgtgaaca gttatctgaa caattggatt atcaggaggc cgacgatcgt
      421 tttgttggta ttgcggcagg gtattgcgct gacgatccag attcggaggt ggcaatcatt
      481 accaatgata gcggtccacg tttttcagca ataaaacata atataatctg ctataaagtg
      541 ccttcttcat ggttgcttcc tgctgagcca gatgaaaagg ataaacaaat taaattgctg
      601 gaagctaagc tgaagcaatt cactcagtct atgcccgatt tcagcattaa agtagccgat
      661 gacgagcaat taaatattgt acttccttat tatacagctt tatctgaata tgaagttgat
      721 aatttgcttt gtcaactaac ggatgcattc ccattggtta caaactttga caatgaacaa
      781 actaccccac aatcactaga aaaaatcatc aagtcattta gtcaacagga gtatttccct
      841 gtatcagcag atgatatttc tctttataaa gaaaaacatt atccactttg gaaaaagcag
      901 tgtgaagaaa tactgaaaaa ctgtcatact catattaatc cgaaggcaat aaattacccg
      961 ctgtcgtttt tattacaaaa cacaggttat actatcgctg aacgtgctgt ggtaacattt
     1021 acagcaaaag gagattttcg tctgtgtgga tttaattggg agataatgga agagctgact
     1081 gaaagcaaaa ggattgacct accttcagcc cctaccgccc ccaaaggaaa atggcgtagt
     1141 gtatgtggcg taacagaaag aatgccttac gctgggggag ctttggaaac accgctatcc
     1201 agtataattt caaaagaaat tggtcc
//
LOCUS       DAZOYS010000413         1216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOYS010000413 DAZOYS010000000
VERSION     DAZOYS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.416"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            63..620
                     /locus_tag="ACHUN9_004435"
     CDS             63..620
                     /locus_tag="ACHUN9_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697634.1"
                     /translation="MASFSNQIKVTNFQIKSTEPIYSNQTWTGQRIMRSTGIQYYQIQ
                     FTLNFNPASLNEVNSFLAQYAQGKPFTMSLGLAGTYYGKQTGAVTATALTKPGNMVVP
                     ISTNTLAVGEWVQFSNHNKLYRIVERTSTSITIFPALQNTVQASEALKYNNLMIEAVL
                     DPDNDYSLPIGNIMNITLKATENII"
     gene            713..>1216
                     /locus_tag="ACHUN9_004436"
     CDS             713..>1216
                     /locus_tag="ACHUN9_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFR0697635.1"
                     /translation="MSLGVNVTCFDVLPKGTNGFHWTDSLIDIDLDGYKYISFPDIIS
                     GSLPSYSEQKGMSNDAINFKISNVNASVRALALGGFLKDAQMNIKLVILNPYDSTVID
                     SMLMFTGFIDYVQAVTDPNQKQNEMTIYVNSVYKKLDRQPALIAANSVYQSYYRNDEY
                     FSLLGQVN"
BASE COUNT          420 a          213 c          215 g          368 t
ORIGIN      
        1 taaaaaaaat acgaatcgag gtttttaaat aaataaagaa agaagatatt cgaggtaaca
       61 aaatggcatc cttttcaaat caaataaaag taacgaactt tcaaattaaa agtactgaac
      121 cgatctattc taatcaaaca tggacaggtc aacgtatcat gagaagtact ggaattcaat
      181 actatcaaat tcagttcacc cttaatttta atcctgcatc actaaatgaa gtgaatagct
      241 ttttagctca atatgcacag ggcaaacctt tcactatgtc attggggctt gcaggtacat
      301 attacggtaa acagactggt gcagtaaccg ctactgctct cactaaaccg ggtaatatgg
      361 ttgtacctat cagtactaac acattagctg tgggtgagtg ggtacagttt tccaatcaca
      421 ataagctata tcgaatcgta gaacgtacca gtacttcaat tactattttc cctgctctac
      481 aaaatacagt acaggcaagt gaagcactca aatataacaa tctcatgatc gaggctgtat
      541 tagatccaga caatgattat tcattgccta ttgggaacat catgaatatt acattgaaag
      601 caacggagaa tatcatctaa tggactcagg tatatatact aatgctaatc ttcttaagta
      661 ctggaagtta gtcagaggta caaccaagac aagattaaca ctaatggaaa ttatgagctt
      721 aggcgttaat gttacttgtt ttgatgtact accaaaaggt actaatggtt ttcactggac
      781 ggattcacta attgatatag atctggatgg ttataagtac ataagtttcc ccgacattat
      841 cagtggttca ttaccttcat attctgaaca aaagggcatg tctaatgatg ctatcaattt
      901 taaaattagc aatgtaaacg cctcggttcg tgctcttgcc cttggtggtt tcttgaaaga
      961 tgcacagatg aatatcaagt tagtaattct caatccatat gatagtactg taattgattc
     1021 aatgctcatg tttacggggt ttattgacta tgtacaggca gtaactgatc caaatcagaa
     1081 gcaaaacgaa atgactatat atgtgaattc tgtctataag aaattagaca gacaacctgc
     1141 attaattgcc gccaactcag tatatcaatc ttactataga aatgatgagt actttagtct
     1201 gttaggtcaa gttaat
//
LOCUS       DAZOYS010000414         1212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOYS010000414 DAZOYS010000000
VERSION     DAZOYS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.417"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUN9_004437"
     CDS             complement(<1..815)
                     /locus_tag="ACHUN9_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0697636.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUN9_004438"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUN9_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUN9_004439"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUN9_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          337 a          209 c          326 g          340 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tt
//
LOCUS       DAZOYS010000415         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOYS010000415 DAZOYS010000000
VERSION     DAZOYS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.418"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUN9_004440"
     CDS             complement(<1..680)
                     /locus_tag="ACHUN9_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0697637.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="ACHUN9_004441"
     CDS             complement(996..>1211)
                     /locus_tag="ACHUN9_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0697638.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAZOYS010000416         1206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOYS010000416 DAZOYS010000000
VERSION     DAZOYS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.419"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..116)
                     /locus_tag="ACHUN9_004442"
                     /pseudo
     CDS             complement(1..116)
                     /locus_tag="ACHUN9_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(303..732)
                     /locus_tag="ACHUN9_004443"
                     /pseudo
     CDS             complement(303..732)
                     /locus_tag="ACHUN9_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(780..>1206)
                     /locus_tag="ACHUN9_004444"
     CDS             complement(780..>1206)
                     /locus_tag="ACHUN9_004444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697639.1"
                     /translation="VMACDYRYKNRQYHCLSGSYMARSAKPRKRKPAPQRSKLLRYVV
                     KLHEDDFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPE
                     IARCTEREFATIPAGISADGMYCPECGTVHWPDGVIPPF"
BASE COUNT          295 a          352 c          243 g          316 t
ORIGIN      
        1 agcagccgat ctaggatcgc tgttgccagc acgtgatctc cgaacatttc tccccagtct
       61 gcgacccctt tgtttgacgt cagtatgatg ctcgcttttt catatcgaca gttcatcgat
      121 aggaatttaa atccccaaaa gattaaaaaa gcaccgaaga atgcgcgttt attcaacaca
      181 acctgatcat caagtaatcg gtatagacac ttaatattgc ttacattgat tgattcaatg
      241 tatctctgat ggttttttgc tccgttcccc tcaacatgga cgcaacttcc cctctgcgac
      301 tctcagccgc accaccgtat tcgagccagc ggctcatgca tcataacctg ctctgccaga
      361 cggtaacccc tcttcagccc cgtaaaccgc atctgacctc cgcacagctc gcacttcagc
      421 gggtcaacct tcagtcactg ctgatacatc cctctccttg tgatttgcct cgccgttttt
      481 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
      541 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      601 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      661 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      721 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      781 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      841 ccgtcggcgc tgataccggc aggaattgtc gcaaattccc gctccgtgca gcgggcaatt
      901 tccggatacc cttcgtcatc aacacgtaca aaccagaaga ccagtttttt gtttccggca
      961 tccacaaaga acggaatatt caggtctgcg caacactcaa cggcatcgtc aaaattatca
     1021 aagcgcagaa cctcagcttc ttcttcgtca aaaaaatcat cttcgtgaag ttttacgaca
     1081 tagcggagga gtttgcttct ttgaggtgca ggttttcgtt tgcgtggttt agctgaacgg
     1141 gccatataac tacctgaaag acaatgatat tgtctgtttt tataacggta atcacaggcc
     1201 atgaca
//
LOCUS       DAZOYS010000417         1194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOYS010000417 DAZOYS010000000
VERSION     DAZOYS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.420"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            70..753
                     /gene="tssD"
                     /locus_tag="ACHUN9_004445"
                     /pseudo
     CDS             70..753
                     /gene="tssD"
                     /locus_tag="ACHUN9_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            733..>1194
                     /locus_tag="ACHUN9_004446"
     CDS             733..>1194
                     /locus_tag="ACHUN9_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697640.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          375 a          236 c          259 g          324 t
ORIGIN      
        1 aaataaaggt aaaaaaaaca acaatactat ttctctttca caaaagaatt aacaaaaggt
       61 ttttcaagga tgagtgatat tatttacctg accataaccg gagaacaaca aggatgtatt
      121 tcctctcgtt gtggaacgag cgcatcaata ggtaatcgct ggcagattgg ccatgatgat
      181 gaaatcttcg cgttctcgct ctctaatagt attacgaata caggcaaagg gtcataattg
      241 catggcctta gtttctgtaa attgattgac aaaagctccc cgttacttat taacgctatt
      301 aacaataacg agcagttgtt catggagttt gacttctatc ggatcaatag gtttggtcga
      361 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
      421 tttactgaga acaatctgga tacagaagcc atcactgtca gttattaata tatactctcc
      481 aggcatctga ttgctaatac tgaatttagt tattgggcat ttcccgataa ctacaaccgt
      541 ctgtttatac cacggccaaa aactaaagct ggtaatggtt tcaaaacact taatagtaaa
      601 gcggttggca gactgcttgc tgctgtcggc atatacaatg ggaatatcga aggattcagg
      661 gaaacagcag acaagttggg gggagatgca cctgctggtt atgatcaatt tatggataac
      721 aaagggttac tcatcgctgg tgcttcaata gttgctggag cagttctggg gagaatgaga
      781 ctccctgatt tggaatcact tgagcatttc ggagccagag gagcagtatc aggaagacca
      841 tttaatcccg agcttgccgg tggtccaata gaaaacctga cgacagatgg cgtaacaatc
      901 aacagggaag gtatcgctat tgttgaaaaa catatagcca gatttggtca tgatcctgta
      961 aatgaagtga tgatcaatag actgaaagac attgagaaag gaaaaatccc cccagaacaa
     1021 gtagatctta acttctatac tcatgagtgt cgggaatatc agcgttactg taatcttggt
     1081 tgggaaacag gacaacctga tggtgacgca ggctatgcac tctggaatca tactggattt
     1141 gcccctatat ttccagacat ctgttatcac ttaacccatt acaagcccgc tgcc
//
LOCUS       DAZOYS010000418         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOYS010000418 DAZOYS010000000
VERSION     DAZOYS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.421"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHUN9_004447"
     CDS             complement(<1..755)
                     /locus_tag="ACHUN9_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR0697641.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHUN9_004448"
     CDS             1071..>1187
                     /locus_tag="ACHUN9_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697642.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZOYS010000419         1185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOYS010000419 DAZOYS010000000
VERSION     DAZOYS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.422"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(9..164)
                     /locus_tag="ACHUN9_004449"
     CDS             complement(9..164)
                     /locus_tag="ACHUN9_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0697643.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(367..774)
                     /locus_tag="ACHUN9_004450"
     CDS             complement(367..774)
                     /locus_tag="ACHUN9_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0697644.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            851..1078
                     /locus_tag="ACHUN9_004451"
     CDS             851..1078
                     /locus_tag="ACHUN9_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0697645.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1062..1185
                     /locus_tag="ACHUN9_004452"
                     /pseudo
     CDS             1062..1185
                     /locus_tag="ACHUN9_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          291 a          243 c          301 g          350 t
ORIGIN      
        1 cgttcatttt actcacctga atgtcttccc aaccaacgac gtgcgccagc ttcggtttta
       61 aacgttttgc ttttggtata cgtcatggcg gtgaatgtgc cgtcctggtt ggggaacacg
      121 ccgtacacca gagattcgtt gttgccaaga tcgatagtat tcatgttgac cccatttccc
      181 cttaacgccg gggtagcgga actgtttgct gagaacaccg tgcggtgtct tgatgaatga
      241 aatttagaat aacctaaggt gggcggtcaa gatttttgtg tagaaaaacc taagtttttt
      301 gatgtaaaaa acacaagtgt ttgaaagttt gtgcttttta ttacagggtg tggagaaaaa
      361 aggggattat ttgtttgcgc ttcttttgcg agctttgagt agttcttcaa aaagtttgtt
      421 gaaattttca actcgagcac gcatctctga caacagggcc ttttgctctg actcaggcag
      481 tgcgtcgaac agttgaagta actctttttg atcttctgtc agaatggctg gctgattatc
      541 cgggatcggt tcgcctggtt gtttatcttc atccccaaaa agaagccaag tcggtgagca
      601 ctgaagcgct tggctcagtg cgaataatct tttccccgcc ggctgtgttt catctctttc
      661 ccattgagaa attgttacgt gagccacttt gaccagctta cctaatgcgg cctgagacag
      721 ttttaatttt ttacgcctat gtaagaggcg agcaccgaag gtttcgtttt tcatattagg
      781 gaattctaat ttttcttgac ttaggtttct ctacaatcta gtttccttag gaaaatctaa
      841 ggagctcgat atgttgaaaa ttgatgctat agcgtttttt ggcagcaaaa caaagcttgc
      901 caatgccgca ggagttaggc tggcaagcat tgctgcatgg ggggaactgg ttcctgaagg
      961 tcgcgcgatg cgcctgcaag aggcatctgg cggggaactt cagtacgacc ccaaagttta
     1021 tgacgaatat cgtaaggcaa aacgaccttg gaaggtgatt catgaaaatc aggcatgagc
     1081 acatcgaatc agtgctgttg ccctggcagc cgaaaaaggg caggcgtggg tcgctaacgc
     1141 aattactgaa gaatatctgc ggcagggggg cggcgaattg tctct
//
LOCUS       DAZOYS010000420         1174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOYS010000420 DAZOYS010000000
VERSION     DAZOYS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.423"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>1174
                     /gene="fimD"
                     /locus_tag="ACHUN9_004453"
     CDS             <1..>1174
                     /gene="fimD"
                     /locus_tag="ACHUN9_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0697646.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQML"
BASE COUNT          332 a          261 c          284 g          297 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg ttag
//
LOCUS       DAZOYS010000421         1167 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOYS010000421 DAZOYS010000000
VERSION     DAZOYS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.424"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..191)
                     /locus_tag="ACHUN9_004454"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="ACHUN9_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            175..738
                     /locus_tag="ACHUN9_004455"
                     /pseudo
     CDS             175..738
                     /locus_tag="ACHUN9_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            768..1167
                     /locus_tag="ACHUN9_004456"
                     /pseudo
     CDS             768..1167
                     /locus_tag="ACHUN9_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          323 a          260 c          334 g          250 t
ORIGIN      
        1 agcatatggt tttgcgggta agccgcttaa gatgtgtccg caggttcaga ttatgtcgct
       61 ctatccgctg agtgcatttc ttgcttataa catgactggt tgaggacaat aaagttcggt
      121 ataccggctc aggcatcggt catgtagaag gcaatgctaa actggctcag caaactaaga
      181 gcagcgcgca tccacttacc caatgcggtt gagaaaatag ccttcgatcg ctttcatgtg
      241 gcgaaacaac cgggtgaggt ggtggataaa acccgccaga atgaacatcc gcatcttcct
      301 gttgaaagtc gtcgtcaggc aaagggaact cgcttcctgt ggcagcacag caataaatgg
      361 atgaccgaat ccaggcagga aaagctgata tggctgcgtg cgcagatgaa gctgacgagc
      421 ctgtgctggg cgctgaaaga actggcaaag gatatctgga gcagaccatg gagcgaggaa
      481 agacgcaacg actggcagag atggctgtcg ctggcggcca acagtgacgt ccccatgatg
      541 aagaatgtgg ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat
      601 ggcgtatcaa acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa
      661 gccaagggct accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa
      721 ctgaatatgg cgttctaagc cttcccacca tgatcgggga agacccagat atgaacaggc
      781 acattcagag ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc
      841 gcagcattgc tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca
      901 cttgtcggtg ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt
      961 ttcggtgatt gtgctgggga agaacagctc tgtacatggc ggcgcttgag gcaccccggt
     1021 ttaatctggt gataaaggcg ttttacatgc gcctacttgc agccggaaac gccaaaaaag
     1081 tggcactggt tgcctgtatg cgtaagttgc tgaccattat gaacgcaatg ctcagaaaaa
     1141 acgaagaatg gaatgaatcg tatttat
//
LOCUS       DAZOYS010000422         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOYS010000422 DAZOYS010000000
VERSION     DAZOYS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.425"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUN9_004457"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUN9_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0697647.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHUN9_004458"
     CDS             complement(510..>1162)
                     /locus_tag="ACHUN9_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0697648.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZOYS010000423         1160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOYS010000423 DAZOYS010000000
VERSION     DAZOYS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.426"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..477)
                     /gene="umuC"
                     /locus_tag="ACHUN9_004459"
     CDS             complement(<1..477)
                     /gene="umuC"
                     /locus_tag="ACHUN9_004459"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0697649.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(477..896)
                     /locus_tag="ACHUN9_004460"
     CDS             complement(477..896)
                     /locus_tag="ACHUN9_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0697650.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          334 a          295 c          240 g          291 t
ORIGIN      
        1 ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccagcgttt tggtctgggc
       61 gatccccata ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc
      121 aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg cctcatcaat
      181 actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat
      241 gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc
      301 tttttgtttg aaccagggat cgcccatttt aacacccagc gcctttgcct cagcgtttcg
      361 ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg
      421 gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagagag caaacatcag
      481 cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg
      541 atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc
      601 aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg
      661 gtaatagcgc tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa
      721 tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga
      781 tcgatgcgct gttcgacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg
      841 ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata
      901 atctgcctga agttatactg tttttatata cagtaatctg ttcttgccag cagatcaata
      961 ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc
     1021 tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg
     1081 gcaaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt gatatttatc
     1141 atattaatag aaataaagac
//
LOCUS       DAZOYS010000424         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOYS010000424 DAZOYS010000000
VERSION     DAZOYS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.427"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUN9_004461"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUN9_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHUN9_004462"
     CDS             572..>1157
                     /locus_tag="ACHUN9_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0697651.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZOYS010000425         1150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOYS010000425 DAZOYS010000000
VERSION     DAZOYS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.428"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            47..382
                     /locus_tag="ACHUN9_004463"
     CDS             47..382
                     /locus_tag="ACHUN9_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0697652.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(663..794)
                     /locus_tag="ACHUN9_004464"
     CDS             complement(663..794)
                     /locus_tag="ACHUN9_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0697653.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(830..1108)
                     /locus_tag="ACHUN9_004465"
     CDS             complement(830..1108)
                     /locus_tag="ACHUN9_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697654.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
BASE COUNT          375 a          292 c          196 g          287 t
ORIGIN      
        1 tatacttttc agaacatcac cagcaaggca taaacaagga aactaaatga agtggattgt
       61 gattgataca gttatccagc catcatgcgg aatatctttt tcagtcatat ggagtaaaat
      121 aaaattaata atctggtatc aatcggatgc tttcttacct cctgaaagta tatttacact
      181 gactcacaca ggcatcatgc tcaataacaa agtgctacct gtaaccattt acaacgtagt
      241 accattcaat aaaacattct ggaattcaat caaaaatagc caggaatgcc ctacaaatac
      301 agataacgta ttgaatgaat gctttaataa ccgttgcact ctgcaaatat gtccttatgg
      361 actaaaacaa caaagtccat aaggagttta ctcacatctg acaaaatcaa tataaacagc
      421 ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc
      481 cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg
      541 cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag
      601 acaacagtca accgcctgaa ttgtgaagta tttaaaaatt tctcccgcta actgataccc
      661 ggctaacagt ctggcgtttt ctttttcagc aacgggaaag caacaaccac cacacccgcc
      721 accagcacac cgtcagccag cactgacatt atccggctgc tgcaatgcca ttcacaaaaa
      781 cagtaagcaa tcacttttta ccgtaacagg tgataatcca gatatgtatc taccccagat
      841 gagtaatccg aagttcatcc ataccacagg tcctggctat tctgttgtac tcctgaacaa
      901 gagcaaataa ttctgaatta gcaaccatga actcatcgca aaccctctgt atagcatcac
      961 tattcagaat aataacgtct cttcccgaaa gacgatcagg agtacagaac aaaactgtca
     1021 aacggctgaa ggcctttgct cgtgctgcat tgactatatc aatacgctgc ctaaggatga
     1081 agcaccccga cgcctcatca atattcactc tacccacacc atatgaatga taaatattta
     1141 atgctgaaaa
//
LOCUS       DAZOYS010000426         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOYS010000426 DAZOYS010000000
VERSION     DAZOYS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.429"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUN9_004466"
     CDS             <1..187
                     /locus_tag="ACHUN9_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0697655.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUN9_004467"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUN9_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0697656.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          276 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgcttt
//
LOCUS       DAZOYS010000427         1143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOYS010000427 DAZOYS010000000
VERSION     DAZOYS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.430"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>1143
                     /locus_tag="ACHUN9_004468"
     CDS             <1..>1143
                     /locus_tag="ACHUN9_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0697657.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVAQMQSQ
                     MDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYDEWGNLLSDENPHHLQQLIR
                     LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLA
                     SKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSP
                     YDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQTNWSTTQL"
BASE COUNT          291 a          304 c          333 g          215 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct ggcatcgtgc ggcctgacgg tggcgcagat gcaaagccag
      481 atggacccgg tatacacgcc ggcgcgaaaa attcacctgt accactgcga ccatcgcggc
      541 ctgccgctgg cccttatcag cacggaaggg acaacagcgt ggtgcgcaga atatgatgaa
      601 tggggcaacc tgctgagtga tgagaacccg catcatctgc agcagctcat tcgcctgccg
      661 gggcagcagt atgatgagga gtccggcctg tattacaacc gccaccgcta ttatgacccg
      721 ctgcaggggc ggtatatcac tcaggatccg attggactga aggggggatg gaatttttat
      781 cagtatccgt tgaatccggt tcagtatata gattcaatgg ggctggcatc taaatatgga
      841 cacttaaata atggcggata tggagcgaaa cccagcaaac cgcctacgcc cgatccaagt
      901 aagttgccag acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt
      961 gcgcctaatg ttttcaaaac attcttcaga gcattaagcc cttacgatta cacactgtat
     1021 tgcaggaagt gggtaaaacc caatctgact tgtatgccgc aggatgatcc ccagtatcca
     1081 gggatggaga caaagacagc aagtgattac ctgccacaga caaattggtc aacaactcaa
     1141 tta
//
LOCUS       DAZOYS010000428         1132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOYS010000428 DAZOYS010000000
VERSION     DAZOYS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.431"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..317
                     /gene="yqeJ"
                     /locus_tag="ACHUN9_004469"
     CDS             <1..317
                     /gene="yqeJ"
                     /locus_tag="ACHUN9_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR0697658.1"
                     /translation="MYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHL
                     TSKKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHC
                     AR"
     gene            complement(351..431)
                     /locus_tag="ACHUN9_004470"
     CDS             complement(351..431)
                     /locus_tag="ACHUN9_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697659.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(467..891)
                     /gene="yqeK"
                     /locus_tag="ACHUN9_004471"
                     /pseudo
     CDS             complement(467..891)
                     /gene="yqeK"
                     /locus_tag="ACHUN9_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
BASE COUNT          409 a          190 c          173 g          360 t
ORIGIN      
        1 ttatgtataa caaaaaaaat aaaacaggaa ctgttgcgtt gagtggaacc tggcaacaag
       61 gtaacagaga aagtaaatca atacggcgaa atattgaata cacatggatt gaaaactatg
      121 acacagccca tttaacatca aagaaagtta ataagtttga gattatggat caagttgatg
      181 atgatagact tgcacaactt attcctgatt tttatgtctt tccagaaaaa agtgtaagct
      241 ataatatact aaagcaaggt aagcatgctt ttattttgag cattggtaac agagcaataa
      301 tgcattgtgc aaggtaaact aaaatagata acaaaaaaga tggaacctcg ttaatcataa
      361 aacaataaaa ttaaatattc tcgcagtata tggcagtcta aagcatcaaa gttttgatca
      421 acatctttca ttttagacat ctccttgcaa tgtaaaatat catgtatcaa caacatctgg
      481 tgcaaatcca tgagtcgaac tcgacattca tcttagctcg attattattc cttcgtacag
      541 tcgatgtaaa caatacagaa agaggattat taagaaccag tttactcagg aatcaataga
      601 ggaaaggact aacgtttctt taaaagaatt gatttcatca tctgttaaac taaactcatc
      661 attgacagat cgtgagatat aactgttttt aactttactc tttacgttga ctttattgac
      721 agaattaaca ttcacatatc ttgaatttaa tgtccatgat gttgtttgcg tgagaacatt
      781 ctcagcatta aggaattttt ttaacggaat ccttggtttc tttttcgagt ccgaatttac
      841 aatatcatgc atatagacca tttcatgaat ctgcgaaaac tcaatgtcca ttgtatacct
      901 cacattttta ccgtgactcg atgttactgt tcaataatca ccttccatca atactaaaat
      961 taatacccct aatgtgccga taacaaatat agtcattcta cgtaacgtct ccataaggtg
     1021 ataaccttga tcaaatgaat ttcgaatata ataaaggaaa tcttttgaga tgtttatcac
     1081 gaataaagct atttatgtta ccaatatcat ttcgagtgcg cacactatct at
//
LOCUS       DAZOYS010000429         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOYS010000429 DAZOYS010000000
VERSION     DAZOYS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.432"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUN9_004472"
     CDS             84..1115
                     /locus_tag="ACHUN9_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0697660.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZOYS010000430         1102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOYS010000430 DAZOYS010000000
VERSION     DAZOYS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.433"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..629)
                     /locus_tag="ACHUN9_004473"
     CDS             complement(<1..629)
                     /locus_tag="ACHUN9_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0697661.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRL"
BASE COUNT          328 a          184 c          217 g          373 t
ORIGIN      
        1 cttaatctat tgctatatgc aaaaagaagt tgcagtctcc taggtaaagg gggcagttca
       61 gtaagatggt tgtgcgcaac cttaagtatc ttaagattag aaggcagaga tggaagagtt
      121 tgcagttcgt tgttcctaac ctcaagtttt tttaaattca agggtaatga aggcagtgtg
      181 gataacctat tatactctgc gcttagtgat ttgatggtgt ctggtaataa cggcagattc
      241 tctaattgat tttcggacac atccaaagtt attagataat gtggtaatgc agatatatgt
      301 gttaagaaat tatctcttgc aataagttct ttaatatttt caggtaaatc agtaaggatg
      361 gttaatttat tacagctcac atcaagcttt ttaatgcaag cagggagtac aggtaacgag
      421 gaaagcgcca aggctcgcag gtccagttta tgccccttag aggtaagaca tgcacgaagt
      481 cttcttaccg cctcagtacg ctgttcttcc ggtaacccct gtttctccca atcatcccat
      541 tcggacaaat aatggttggg ggatgtaatt gtgctgatat gtggaaataa acaggatgaa
      601 atatttctga gcatattaat ttctcgcata tggatatgga attagattgt gaataaattg
      661 tatttaatgt atctctccta atgaggaggg tgattcagaa tattcgccat aagggaatga
      721 gtgttatatc atgctttcta ccctggtaag tatatttgct tttttcgtat atttggttca
      781 tctttttttg atgttttaca tattcacaat gcagaggatt tataccagaa gacattagtc
      841 ttgagtaaga tactacaaca tctccaacta ttttagccag cctgactact tgacttggta
      901 atatttgttg ctaataatca tgtataaaat aagcttatat acatagtaac ttcagcaaag
      961 gatttagtaa tgatccaaat catatctcaa tgtactgttt ttttctttaa aaaaacagta
     1021 cattctactt tataatgact gtgtattccc atggaaggag atcacccaat tctccccccc
     1081 gacttttgac gcagtgtctt gt
//
LOCUS       DAZOYS010000431         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOYS010000431 DAZOYS010000000
VERSION     DAZOYS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.434"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            82..255
                     /gene="ymgI"
                     /locus_tag="ACHUN9_004474"
     CDS             82..255
                     /gene="ymgI"
                     /locus_tag="ACHUN9_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0697662.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            257..601
                     /locus_tag="ACHUN9_004475"
     CDS             257..601
                     /locus_tag="ACHUN9_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0697663.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            605..940
                     /locus_tag="ACHUN9_004476"
     CDS             605..940
                     /locus_tag="ACHUN9_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0697664.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          318 a          227 c          261 g          285 t
ORIGIN      
        1 attgataaat cagattatct tacctttaca acattgcgtt aacagtcaat gatacttctt
       61 gaataaaaat attgccaatc aatgagttat tcctcattta agattattct cattcacgta
      121 aaaaacataa ttcctatcat cacagccacg ctcattttga attatctgaa taatagcgag
      181 cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta
      241 ataccggaca tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt
      301 ctgctccacg ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat tgctaggcgc
      361 aggcgttgga ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
      421 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcac gtaaaggtgc
      481 aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
      541 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
      601 aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac
      661 ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc
      721 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtgat
      781 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
      841 caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac
      901 ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttaatat gttaccgcgt
      961 cgatgaattc gacgcggtat tttagctaac ggggatcaga acttatattt caggcccacc
     1021 atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt accccagaca
     1081 ctggccgccg g
//
LOCUS       DAZOYS010000432         1090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOYS010000432 DAZOYS010000000
VERSION     DAZOYS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.435"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            265..897
                     /locus_tag="ACHUN9_004477"
     CDS             265..897
                     /locus_tag="ACHUN9_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0697665.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          378 a          186 c          198 g          328 t
ORIGIN      
        1 tggtcattca aaaacaattt gcgatataga tattctcata ccatgagata gttatctgga
       61 ccacgttcga ccatgcaaga ttaaatcagt gaaacataat catatttaat aacgagaaat
      121 gcatttttaa atgtcttaat cagaattata gcaaatacag attaatcgca atttttccca
      181 gcaaaattac aaaatcaaga tgtattgaca tcatcaatca ccaatgaaat tatgcaatta
      241 tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag tcttacatgc
      301 aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt gcagtattgc
      361 gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat attttaatcg tggtgtggtg
      421 gaaggtatct taacaaaaaa ccacaatgcg agattaagcg gatatatttt tgtggatttt
      481 tcagtaagtt ttttacgtct attccttgaa aaagactgga ttgactatct tgcaagtact
      541 gacatgggga tagttttagt cagcgaccgc aatatgcaat cgttagctaa ctactggcga
      601 aaacacaaca ccgcaatatc tgctgtcata tataatgatg atggtcttga tgttgccaat
      661 gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg taacacatta
      721 actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc atatcagatt
      781 gctgaagtgc tggatatgga tatccgtagc atctacgcgt acaagcaacg aatcgaaaag
      841 agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca acattgatac
      901 tcaacgacca gccagaatca tactctgttc atacgggcat ctattgaaat gcccgtattt
      961 tataaataag cagtaacccc aagagtaaac aacatagtat ttaaaaccat cacacgaaaa
     1021 attcagaagc attacgaaat atgaatttac aaacaatagt ggcataaatg ttaaccatgt
     1081 taatttacgt
//
LOCUS       DAZOYS010000433         1089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOYS010000433 DAZOYS010000000
VERSION     DAZOYS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.436"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            530..>1089
                     /gene="fimB"
                     /locus_tag="ACHUN9_004478"
     CDS             530..>1089
                     /gene="fimB"
                     /locus_tag="ACHUN9_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR0697666.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          190 c          233 g          333 t
ORIGIN      
        1 ctccaggata aaaaggaatg taacaatctc atggcgtaag ctgacgaacc aggcaggaat
       61 aatcgctagg gacctaagca ttagcatgat aatagccact aagaaattac tgtgttccat
      121 gaaatagcca ttttgtggca aatggagttg actaataatg tcatatgtga gacggctagt
      181 tgaacgaata ttaaattttg ctgaattttt tatgttgatt ttacttgtta cagaacatat
      241 cacatgatat atagataaga ttagttgcat taatgatgag ggttattatt agattcgtat
      301 caggttgata aatatataaa ggtacatagc atgcaagagc atggcgtttg tatggcaaag
      361 ttattataag taactatcgc tactccattt aagttcgctc agaagaactg gtccacttac
      421 gttagttatt aagcaaacgt tcgcttttat aaacataatc aggattaaaa tgttggatta
      481 ttgctaaccc agcacagcta gtgcgcgtct gtaattataa gggaaaacga tgaagaataa
      541 ggctgataac aaaaaaagga acttccttac ccatagtgaa atcgagtcac tccttaaagc
      601 agcaaatacc gggcctcatg cagcacgtaa ttattgtctg actttgcttt gttttattca
      661 tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg gatattgatc ttaaggcaaa
      721 gtgtatatat atccatcgat taaaaaaagg cttttcaaca acgcacccgc tattgaacaa
      781 agaagttcag gctttaaaaa actggttgag tatccgtact tcttatccgc atgctgagag
      841 cgagtgggta tttttatcac gtaaggggaa tccgctttct cggcaacagt tttaccatat
      901 tatctcgact tccggtggta atgccgggtt gtcactggag attcatccgc acatgttacg
      961 ccattcgtgt ggttttgctt tggcgaatat gggaatagat acgcgactta tccaggatta
     1021 tcttgggcat cgcaatattc gtcatactgt ctggtatacc accagcaatg cagggcgttt
     1081 ttacggcat
//
LOCUS       DAZOYS010000434         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOYS010000434 DAZOYS010000000
VERSION     DAZOYS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.437"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..1037)
                     /locus_tag="ACHUN9_004479"
     CDS             complement(<1..1037)
                     /locus_tag="ACHUN9_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0697667.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKT"
BASE COUNT          347 a          213 c          164 g          364 t
ORIGIN      
        1 gttttaagat tgactaaatt acttgcatct tcacgtacca tcgtggcaac tggaacaggc
       61 agagacatga aaacttcttc attcaccatc aagtgaatca tgcattgcgt tccctttgtc
      121 atatccacac atacgctaca aatatcgtgg taatgcgaag agcgcatttc aggaaacaga
      181 ttaaacacgg tatcttgaaa ggcactaaat cgtgttaata aatcctgata atgaacaggt
      241 tttgtttcaa aattatcatc agcaaatcca agcactattg tatgctctac atctaacgca
      301 gtttcaaatg gctcttcttc tgtaaaatca ttatttaaaa cctcacctga cgtattatca
      361 ctgtcataaa aagtgttcat caatgcgata ttatttgtta cagcgtcttt ttcgatattc
      421 tcactaattg catatcgcct gctataatca taaaaatgaa taaacgcgcc ctttataaag
      481 gatatatatt gttctttgtt aaagtcaata cggctgtata atttttcaaa aatcacattc
      541 gcaattttac taccaatggt atgcctgtat aataatgggt gcgattcaat cgtaaaatgc
      601 ttgtctaatt gatcttgtaa tttattctca tagtccagag ggaaaagaaa ctgttgtctt
      661 tcaagtttcc cattcgatac attacgtatt ccaatagaga ttctttcatt acccgccttt
      721 atttcaactt cgttatcttc tgatttaccc agatattttt taacatcagt ctcgaaagca
      781 ttactaaaaa aagtattaaa ttggtaattc gctgatggca tattttccgc gagcaacaca
      841 tcatttttta tgagatcact gtcagcattt attgctaata tcagatgcaa gtagatggta
      901 tcccatgtag acaactcacc cttacgactg gaatttaata tcgcgaaaaa ttccctcagt
      961 gaatccagga aattatcact ttgatttagt tgtgtgcctg gaaatgatga tgcttgatca
     1021 gatatattgt ttatcatatt aaaaatcacc caagaaaaac gataatccac cgaactcctc
     1081 gtgcttta
//
LOCUS       DAZOYS010000435         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOYS010000435 DAZOYS010000000
VERSION     DAZOYS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.438"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHUN9_004480"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHUN9_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHUN9_004481"
     CDS             294..>1087
                     /locus_tag="ACHUN9_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697668.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZOYS010000436         1081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOYS010000436 DAZOYS010000000
VERSION     DAZOYS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.439"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUN9_004482"
     CDS             complement(<1..643)
                     /locus_tag="ACHUN9_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0697669.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1081)
                     /gene="tabA"
                     /locus_tag="ACHUN9_004483"
     CDS             complement(766..>1081)
                     /gene="tabA"
                     /locus_tag="ACHUN9_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0697670.1"
                     /translation="LISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGTP
                     DTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKML
                     MA"
BASE COUNT          309 a          247 c          191 g          334 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 a
//
LOCUS       DAZOYS010000437         1073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOYS010000437 DAZOYS010000000
VERSION     DAZOYS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.440"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            144..830
                     /locus_tag="ACHUN9_004484"
     CDS             144..830
                     /locus_tag="ACHUN9_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0697671.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(921..1057)
                     /locus_tag="ACHUN9_004485"
     ncRNA           complement(921..1057)
                     /ncRNA_class="other"
                     /locus_tag="ACHUN9_004485"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT          288 a          227 c          262 g          296 t
ORIGIN      
        1 cgcaatggct aaatagggat ggttgtaata atcaaaacat gatactaact atatattgat
       61 cgttgcattg ttggcatgta acacgctgca gcgagtaaca ttggtaatga aatataatca
      121 acatggatag gtgaaaatta attatgagct ttatggcttt tgtaacttgt ttgacaattg
      181 catttatcgc cggttttgct gttgccagaa aattgacact ccgaaaagtt gcctcatcgg
      241 taatcatcgc taaatcgcaa cccgtccctt catctgcaac ttcccctgcc gctaaggcgt
      301 cgacaaggag ctatataccg cagaaaacct atcgtgactc tcgttggcaa gagcgagatg
      361 agttatttca gggaatggtt gccgaaggta ttttattgga aagaaaagag tggttaatca
      421 cgcgttgtga aaaaatgtat ttcgataatc tggaaaaatg gtttggacat tattgtcata
      481 tccattgcca ggtctctctg gggcgcttga tcaactttcc agaacaacct gagtttagcg
      541 aagaagaaag aaagcgtttc tttgcaattt ataatggcat ggcaatggat tatgttctgg
      601 tttcaaagaa gaacaataaa atagtctgtg ttattgagct ggatgacccg agtcatgaaa
      661 gggcggatcg cattattcgt gaccgttggt tggatacaat tatgactctt gcacgggtgc
      721 cttttgtacg agcttctgtc actaaattgg aggaaattcc tccggtctgg gagactcgtg
      781 acaaaattcg cccattagtc gcggcgacaa agagtgaacc ggtgcattaa ctaagtatcc
      841 cggtcggggc ttctcatccc cccggtgtgt gcaatatacg aaaaaaagcc cgtaccttcg
      901 tacgagctct tctttaaaga tggcggtgag ggggggattg actcgcttcg ctcgccctgc
      961 gggcagcccg ctcactgcgt tcacggtctg tccaactggc tgtcgccagt tgtcgaaccc
     1021 cggtcggggc ttctcatccc cccggtgtgt gcaatatacg aaaaaaaagc ccg
//
LOCUS       DAZOYS010000438         1064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOYS010000438 DAZOYS010000000
VERSION     DAZOYS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.441"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>1064)
                     /locus_tag="ACHUN9_004486"
     CDS             complement(<1..>1064)
                     /locus_tag="ACHUN9_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0697672.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDEKG"
BASE COUNT          164 a          412 c          294 g          194 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt ccggcagtat cactgaagtc
      121 agctttccag acgccatatg cagtcggtac tccgtccgcc gtcccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcagct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcgc
      481 acctgcatcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaggaaggag ctgagagagg gcagggactg tcaggggaag ataacgaagc ggtgtgcggt
      721 ttacgggcct cttccgcccg ctgcgcctgt gtggtcagca ccaggtgaaa acggcggccc
      781 gcgccgtcca tcacccccgt caccgcgccc gccacgtcac cctccgctgc ccggtgaaag
      841 gccagcgtcc gcccgaagcc gtccaccacg cccgtcagca cccggtatgc gggcggctcc
      901 ggcggcagca cctcgtccgc ccccggcacc cgctccggcc agctgagtat ccaccacggc
      961 ccctgaaggc tgtttgtcgc caggtacaca tgcggactca ggcgaacatc ttccggcagc
     1021 acctgccaga gcgcgcttag cggctgcgaa ctgtgctgcg ccgc
//
LOCUS       DAZOYS010000439         1051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOYS010000439 DAZOYS010000000
VERSION     DAZOYS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.442"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            63..>1051
                     /gene="phoE"
                     /locus_tag="ACHUN9_004487"
     CDS             63..>1051
                     /gene="phoE"
                     /locus_tag="ACHUN9_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0697673.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQL"
BASE COUNT          309 a          215 c          265 g          262 t
ORIGIN      
        1 tcctgttttc aacggggctc tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga
       61 aaatgaaaaa gagcactctg gcattagtgg tgatgggcat tgtggcatct gcatctgtac
      121 aggctgcaga aatatataat aaagacggta ataaactgga tgtctatggc aaagttaaag
      181 ccatgcatta tatgagtgat aacgccagta aagatggcga ccagagttat atccgttttg
      241 gttttaaagg tgaaacacaa attaacgatc aactgactgg ttatggtcgg tgggaagcgg
      301 agtttgccgg aaataaagcg gagagtgata ctgcacagca gaaaacgcgt ctcgcttttg
      361 ccgggttgaa atataaagat ttgggttctt tcgattatgg tcgtaacctg ggcgcgttgt
      421 atgacgtgga agcctggacc gatatgttcc cggaatttgg tggcgattcc tcggcgcaga
      481 ccgacaactt tatgaccaaa cgcgccagcg gtctggcgac gtatcggaac accgacttct
      541 tcggcgttat cgatggcctg aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg
      601 acgttaaaaa gcaaaacggc gatggcttcg gcacgtcatt gacatatgac tttggcggca
      661 gcgatttcgc cattagtggg gcctatacca actcagatcg caccaacgag cagaacctgc
      721 aaagccgtgg cacaggcaag cgtgcagaag catgggcaac aggtctgaaa tacgatgcca
      781 ataatattta tctggcaact ttctattctg aaacacgcaa aatgacgcca ataactggcg
      841 gctttgccaa taagacacag aactttgaag cggtcgctca ataccagttt gactttggtc
      901 tgcgtccatc gctgggttat gtcttatcga aagggaaaga tattgaaggt atcggtgatg
      961 aagatctggt caattatatc gacgtcggtg ctacgtatta tttcaacaaa aatatgtcag
     1021 cgtttgttga ttataaaatc aaccaactgg a
//
LOCUS       DAZOYS010000440         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOYS010000440 DAZOYS010000000
VERSION     DAZOYS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.443"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            35..697
                     /locus_tag="ACHUN9_004488"
     CDS             35..697
                     /locus_tag="ACHUN9_004488"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0697674.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
BASE COUNT          307 a          218 c          204 g          317 t
ORIGIN      
        1 tcaagagtga ttgcaataaa aatttttatt tcttatgtca attgagcata ttttaatcgg
       61 gaagcatcat gggggttctg attactggag aactccattt ttgttattta agaacttaca
      121 ccgtgaattt attttcagtc tagatggtgc tgcaactgag catgacacgc tgttaccacg
      181 attcaccgat gatataagcc gtcaatcatg ggttagtgaa aaggtcttct gtaacccgcc
      241 gtacagcgat attccgtcgt tcctcttaaa agcgtctgaa gcggatttag tagctttcct
      301 gataccgcat agagccaaca ccagctactg gcttaggcac atttacagca ataaccactg
      361 tcatgaaatc cgtattcttc atcgtgcagt taaatacctt ccaccagcag ggcataacag
      421 gcttactatt aggtcaccgt ttccaagtgc agtcgttgtt tttaaaaaag aaccacgtaa
      481 gcatgaaatt acacagatgg tttgctgtgc tgatacattg ctaccgttaa ccatcattaa
      541 ccgtggtgga ctaagaggaa gacccaccat ttacccacct gagacgctcg acagcttcat
      601 aaaactatac aggcaaggaa aacccattaa gtacattgca gatgctctca gaatgcctct
      661 gagcacgtca tacaggattg ctcaaaggtt gagctaaaat tctcataaat atggtcattt
      721 acaagacagg tgattcagat gttaagaagt atccctgtga acaataaatg accaatcagt
      781 aatcattctg tgaaataatt gttgcaattt ataatcacat ctgttatagt ttctatatgg
      841 gctgataaaa aagttcatgt cttgaagtgg taacacttgg ggaggtgtca gatggttggc
      901 gtatattaac ctctcattca gggtatacct cagcgtttta atcatcgtac tgtttcaggc
      961 atggacttct ttatcagtcc atcaatcccc cagatatttt aatcacacag gctaatcggt
     1021 tttatccggt tggccttttc acgtat
//
LOCUS       DAZOYS010000441         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOYS010000441 DAZOYS010000000
VERSION     DAZOYS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.444"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHUN9_004489"
     CDS             <1..120
                     /locus_tag="ACHUN9_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0697675.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHUN9_004490"
     CDS             120..410
                     /locus_tag="ACHUN9_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0697676.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHUN9_004491"
     CDS             407..961
                     /locus_tag="ACHUN9_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0697677.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          298 a          200 c          298 g          248 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccg
//
LOCUS       DAZOYS010000442         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOYS010000442 DAZOYS010000000
VERSION     DAZOYS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.445"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            81..296
                     /gene="xisR"
                     /locus_tag="ACHUN9_004492"
     CDS             81..296
                     /gene="xisR"
                     /locus_tag="ACHUN9_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR0697678.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            298..>1042
                     /locus_tag="ACHUN9_004493"
     CDS             298..>1042
                     /locus_tag="ACHUN9_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR0697679.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          352 a          192 c          243 g          255 t
ORIGIN      
        1 aaaacttcat agctgattat taataatcaa catcgggcgt caatttcagc ctaacattgg
       61 cgcctgccag aggtgatgcg atggcacaag taatctttaa tgaagagtgg atggttgaat
      121 acggcctgat gcttcgcact ggtctgggtg ccagacaaat tgaagcatac cgccagaact
      181 gttgggtgga aggcttccac ttcaaacgag tatctccttt agggaagcca gacagtaagc
      241 gagggattat ctggtacaac tatccgaaga taaatcagtt tatcaaagac tcatgatatg
      301 tctaaattac caacaggtgt cgagattcga ggtaaataca ttcgcatctg gttcatgttt
      361 cgaggaaaac gatgtcggga aacattgaaa ggctgggaga ttactaacag taacattaaa
      421 aaggccggga atttaagagc gttgatagtt catgaaatca gttccggtga atttgagtat
      481 ttaagacgtt ttccccagtc cagcactggg gcaaaaatgg tgacaacgag ggtcataaaa
      541 acattcgggg agctttgtga tatctggaca aaaattaaag agacagagtt aacaacaaac
      601 acaatgaaga aaacgaaatc acaattaaaa acactcagga taataatttg tgagagtacc
      661 ccgatatcgc atattcgtta tagcgatatc ttaaactacc ggaatgaact gctgcatgga
      721 gaaacgcttt acctagataa tccaagatcc aacaaaaaag gaagaaccgt gcgcacagtt
      781 gataactata tcgccctgct ctgttcgttg ttacgttttg cgtatcagtc gggatttata
      841 tcaaccaaac catttgaagg agtaaaaaaa ttacagcgaa acagaataaa gcctgacccg
      901 ttatctaaaa cagaattcaa tgcattaatg gaaagtgaaa aaggacagag ccaaaacttg
      961 tggaaatttg ccgtttactc cgggcttcgt cacggggaac tggcagctct ggcgtgggag
     1021 gatgtggatt tcgagaaggg aa
//
LOCUS       DAZOYS010000443         1037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOYS010000443 DAZOYS010000000
VERSION     DAZOYS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.446"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUN9_004494"
     CDS             complement(<1..906)
                     /locus_tag="ACHUN9_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0697680.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          293 a          236 c          207 g          301 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagt
//
LOCUS       DAZOYS010000444         1031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOYS010000444 DAZOYS010000000
VERSION     DAZOYS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.447"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>1031)
                     /locus_tag="ACHUN9_004495"
     CDS             complement(<1..>1031)
                     /locus_tag="ACHUN9_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR0697681.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDRQG"
BASE COUNT          172 a          384 c          284 g          191 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg c
//
LOCUS       DAZOYS010000445         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOYS010000445 DAZOYS010000000
VERSION     DAZOYS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.448"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUN9_004496"
     CDS             complement(77..865)
                     /locus_tag="ACHUN9_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0697682.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUN9_004497"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUN9_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0697683.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZOYS010000446         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOYS010000446 DAZOYS010000000
VERSION     DAZOYS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.449"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHUN9_004498"
     CDS             <1..164
                     /locus_tag="ACHUN9_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0697684.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>1009
                     /locus_tag="ACHUN9_004499"
     CDS             170..>1009
                     /locus_tag="ACHUN9_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0697685.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          306 a          229 c          246 g          228 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAZOYS010000447         1008 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOYS010000447 DAZOYS010000000
VERSION     DAZOYS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.450"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..126
                     /gene="rcbA"
                     /locus_tag="ACHUN9_004500"
     CDS             <1..126
                     /gene="rcbA"
                     /locus_tag="ACHUN9_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR0697686.1"
                     /translation="SKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLLSS"
     gene            231..455
                     /locus_tag="ACHUN9_004501"
     CDS             231..455
                     /locus_tag="ACHUN9_004501"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0697687.1"
                     /translation="MYLTLKEWNARQLRPRSPETVRRWVRECKIFPPPIKDGREYLFH
                     ESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            346..>1008
                     /locus_tag="ACHUN9_004502"
     CDS             346..>1008
                     /locus_tag="ACHUN9_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0697688.1"
                     /translation="MNIYFMSQQKKLHLKRLAGFCRESEMAGRRRNSNTRDLPPNLYN
                     RGGYYSYRDPRSGREFGIGRDKRIAVNEAIAANMELLSTQHISLLDRIKGNSVTLFCE
                     LILNFRNEIERRQLSTNTMKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSK
                     FAIANQYRALLSDIFKTAIASGLAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADV
                     QNE"
BASE COUNT          357 a          184 c          219 g          248 t
ORIGIN      
        1 tcaaaatcta aactaacgaa atcagaatgc gaaaaaatgc tctcagggaa aaaagaagca
       61 ggcgtttcca gagagcagaa agtaaaactg ataaatttta attgcgagaa acttctgtcc
      121 tcgtgagttg cattatatca aaattagaac ttcatagctg attattaaaa atcaaccacg
      181 cccgccagta ttttgtatat ttactggcgg tcatattaca ggaatatgac atgtatttga
      241 cactcaaaga atggaacgcc cgtcagttac gtccacgaag cccagaaacg gttcgccgct
      301 gggtgcgaga atgtaagatt tttccaccac cgataaagga tgggcgtgaa tatttatttc
      361 atgagtcagc aaaaaaaatt acacctcaaa cgactggcgg gcttttgcag agaatcagaa
      421 atggccggac gaagaagaaa ctcaaacaca cgtgatctac cacccaatct ttacaatcga
      481 ggtggatatt atagttatcg tgacccacgt tcaggaaggg agtttggaat cggaagagac
      541 aaaagaattg cagttaatga ggctattgcg gcaaatatgg aattattatc cacgcaacat
      601 ataagcttac tggatagaat aaaaggtaat agcgtaacat tattctgcga gctgatatta
      661 aatttcagaa atgaaataga gcgtagacaa ttaagcacaa acaccatgaa aagacacaac
      721 cagaggttga aaatcataag tgaatacttt ggtggtgttc cggttaaaaa tataggcata
      781 agggaggtgt acagctttct tgaaatcaga gcagctggga gtaaatttgc tattgctaac
      841 cagtacaggg cgcttttatc agacattttt aaaacagcta tagcttctgg tcttgccgaa
      901 gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag tcgactgtta
      961 attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaa
//
LOCUS       DAZOYS010000448          995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOYS010000448 DAZOYS010000000
VERSION     DAZOYS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.451"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            68..829
                     /gene="ydeO"
                     /locus_tag="ACHUN9_004503"
     CDS             68..829
                     /gene="ydeO"
                     /locus_tag="ACHUN9_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0697689.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT          310 a          187 c          191 g          307 t
ORIGIN      
        1 cagcaaatta taaatatgaa atgttaaaaa agtatcgata aaaactttat tgttttaagg
       61 agataaaatg tcgctcgttt gttctgttat atttattcat catgccttca acgctaacat
      121 tttagataaa gattacgcct tctctgacgg cgagatcctg atggtagata acgctgttcg
      181 tacgcatttt gaaccttatg agcggcattt taaagagatc ggatttactg aaaataccat
      241 taaaaaatat ctacaatgca ctaacatcca gacagtgacg gtgcctgttc ctgcgaagtt
      301 tttacgtgct tcaaatgtac cgactggatt gcttaatgaa atgattgctt atcttaactc
      361 ggaagaacgc aatcatcata atttttcaga acttttgctt ttttcttgcc tgtctatttt
      421 tgccgcatgc aaaggtttca ttacactatt aactaacggt gtgctatccg tttccggaaa
      481 agtgagaaat attgtcaaca tgaagctggc gcacccatgg aagctgaaag atatttgtga
      541 ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta aagcaagagc aaacgacatt
      601 ctcacagatt ctattagatg ccagaatgca gcacgcaaaa aatttgatac gcgtagaagg
      661 ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt acatcttatt ttatttatgc
      721 gttccgcaaa catttcggca acagtccgaa gagagtttct aaggagtacc gttgtcaaag
      781 tcacatgggt atgaatacgg gcaacacgat gagtgcttta gctatttgat tatttgctaa
      841 cgagtagtca accacgcacg ctgcgtaaga attaaatggg gcagccattc cctgccccgc
      901 gttgttttta ggcgatatat ttattgaaat aaataagtga catccatcac atatttatgc
      961 acttgcataa cctgttgcag attatttatg atctc
//
LOCUS       DAZOYS010000449          985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOYS010000449 DAZOYS010000000
VERSION     DAZOYS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.452"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            230..>985
                     /gene="gspA"
                     /locus_tag="ACHUN9_004504"
     CDS             230..>985
                     /gene="gspA"
                     /locus_tag="ACHUN9_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0697690.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          237 g          269 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag ggcattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgt
//
LOCUS       DAZOYS010000450          969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOYS010000450 DAZOYS010000000
VERSION     DAZOYS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.453"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(178..849)
                     /gene="queE"
                     /locus_tag="ACHUN9_004505"
     CDS             complement(178..849)
                     /gene="queE"
                     /locus_tag="ACHUN9_004505"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0697691.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          263 a          236 c          211 g          259 t
ORIGIN      
        1 aaactttacc gcaataattt tcactccagc gaaaaattaa tgccacagaa tttgtagaga
       61 atgctaatga ttagccctgg atgggtaaaa tggaggagtt ttcagaggcg acaaacaata
      121 taaatgagta gaagtcttta caggttccac ccatttataa cgcttataaa tgtttaatca
      181 ggcaatattt agatatttat gtgtttgcat cgacaaacgc caattacgcg caatgcaggt
      241 ttcaatgcac aaacgtgtgg catcatcctt ttggctaatc ggctgcagtg caatgactcg
      301 cggtttatca tcggtcagcg ttgccaatag ttcatccagt gcttcaatat cgcgtacgcg
      361 ccccactgga tgcttgattt cgttggctcg ctccagtgcc tgtgacaaca cgtcatagcc
      421 gccgcgcatg ttcagctttg gcgatacggt aacccaggta ttcggtgtgc agcgtacctc
      481 atgagtacca ctggtttcga tctggcagct aaaaccgttc ttttcgagca gatcagtcag
      541 tggtagcaaa tcatgaatgc aaggctcacc acccgtaatc accacatgcc gcgcggtgta
      601 tccctggcga ctaatgacag ccaacaagtc ttcactgctc gccgcccccc acttatcact
      661 ctctttggtc ttcgccagaa tgctgaaaag ggagacttcc cgatcctcaa gcttttccca
      721 ggtgtgtttg gtgtcgcacc aggcacagcc aaccgggcat ccctgtaaac gaataaaaat
      781 ggcggggacg ccggtaaagt aaccctcacc ttgcagggtc tggaacatct cgttaatcgg
      841 gtactgcata gcattctctg tgaagtggat aattgttaat tattgcagat cctgccacaa
      901 caatcatgtc ttattaacat tctgttacag gcagatttaa agaaaaatgc gaaacatatc
      961 gttaataat
//
LOCUS       DAZOYS010000451          967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOYS010000451 DAZOYS010000000
VERSION     DAZOYS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.454"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            211..>967
                     /gene="istA"
                     /locus_tag="ACHUN9_004506"
     CDS             211..>967
                     /gene="istA"
                     /locus_tag="ACHUN9_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0697692.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAE"
BASE COUNT          251 a          256 c          229 g          231 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaa
//
LOCUS       DAZOYS010000452          960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOYS010000452 DAZOYS010000000
VERSION     DAZOYS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.455"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            109..723
                     /gene="evgA"
                     /locus_tag="ACHUN9_004507"
     CDS             109..723
                     /gene="evgA"
                     /locus_tag="ACHUN9_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0697693.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            728..>960
                     /locus_tag="ACHUN9_004508"
     CDS             728..>960
                     /locus_tag="ACHUN9_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFR0697694.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQR"
BASE COUNT          302 a          184 c          202 g          272 t
ORIGIN      
        1 ttaaaggaag ctcagatttt cttattttta ttgagaaaat gagatgacgc cttatgtctg
       61 tattactaca gggagaaggg agatgcttca ttgcaaaggg aataatctat gaacgcaata
      121 attattgatg accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat
      181 attgaaatct tagcggagtt aactgaaggc ggaagcgccg ttcagcgggt ggaaacactt
      241 aagcctgata tcgtcatcat tgatgtcgat atccccggag ttaacggtat cctggtgtta
      301 gaaacgctga ggaagcgcca atatagcgga attattatta tcgtctccgc taaaaatgac
      361 catttttacg ggaaacattg tgctgatgct ggcgctaatg gtttcgtgag taaaaaagaa
      421 ggcatgaaca atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc
      481 ttctctctca accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta
      541 tcgaaacaag aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt
      601 gcggaaaaaa tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa
      661 aaattagaat gtaaatcact gatggatctt tacacattcg cacaacgtaa caaaatcggc
      721 taaccacatg aagtttttac cctatatttt tcttctctgt tgtggtcttt ggtcgaccat
      781 aagtttcgca gacgaagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcat
      841 acttgatcca gtacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct
      901 tgtgattgca gtacataagt cccaaacggc tacgttgttg cataccgatt cgcagcaacg
//
LOCUS       DAZOYS010000453          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOYS010000453 DAZOYS010000000
VERSION     DAZOYS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.456"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(197..>935)
                     /locus_tag="ACHUN9_004509"
     CDS             complement(197..>935)
                     /locus_tag="ACHUN9_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0697695.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DAZOYS010000454          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOYS010000454 DAZOYS010000000
VERSION     DAZOYS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.457"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(341..>921)
                     /locus_tag="ACHUN9_004510"
     CDS             complement(341..>921)
                     /locus_tag="ACHUN9_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0697696.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          281 a          183 c          165 g          292 t
ORIGIN      
        1 taacgtataa actagattta ataataacga actagattta ataacaacga tatccgtacc
       61 tatccggata tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaactt
      121 tcccactctt gaactccaca tagtccaaac gccggatgca aaaaaatcac acgtgcattt
      181 ctgaaattgt gatggaggaa gatagtatca attatatgtg cttataaacg tccccgatta
      241 gcgcagaagt caccggattg ttcaggctcc aataggttga gtatgccgta tttattttcc
      301 aaaataaaaa tcgatgctgt tgctccttca taaaatactt ttactctctg gacgcttttc
      361 ttagctctct tggtgttatc ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac
      421 acttgtaacc attttccctg gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag
      481 acaacgcatt atttaacctc acgtcgtcca atatactttg aaaacttact ccctcgcttg
      541 ccagacggcg atgcaatgtg gaaacagaaa tgtagagata tctcgctacc ttgtttgctg
      601 tccattttgt tccaggttcg gatagcatta ggttataaca acgacttatt aatgattgtt
      661 tactatctga taaaagtaaa caattaacat gatgtgctcc taatgaaagt agaacgccca
      721 ttgctaagtg ttcctgaatt tgcgttgaga agcctcggga aatagatgtt tttagttgct
      781 cccaacaata tattaactcg gaattctgag gtaagcagaa acctgtcgtg ttacgaattt
      841 gatcggttat cgcataaaga ttttggaact tttcaattag atcaatggga taataaagca
      901 tttctgcaag ataaagacgg t
//
LOCUS       DAZOYS010000455          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOYS010000455 DAZOYS010000000
VERSION     DAZOYS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.458"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHUN9_004511"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHUN9_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0697697.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          267 a          146 c          229 g          279 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg a
//
LOCUS       DAZOYS010000456          920 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOYS010000456 DAZOYS010000000
VERSION     DAZOYS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.459"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..160)
                     /locus_tag="ACHUN9_004512"
     CDS             complement(<1..160)
                     /locus_tag="ACHUN9_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0697698.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYG"
     gene            168..599
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004513"
                     /pseudo
     CDS             168..599
                     /gene="tnpA"
                     /locus_tag="ACHUN9_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          267 a          214 c          187 g          252 t
ORIGIN      
        1 tcccgtaagg gatgtattta ttaccgacct catgattctc attctgacgt gccagagcac
       61 gattgaaaac gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac
      121 cgggtcttaa ctggaattta aatgcttgta gtcgtttcac aggccaaatg atactttggt
      181 ctgtgaaaaa agaaaccgat atccggcgtg gaaggcattg tgttttcctg atgcatgttc
      241 acctggtctt tgtcaccaaa taccgacgtc agatttttga ctatgacgcg acagaaaaac
      301 tacgcactta cttttcaaat gtatgtgctg attttgaagc tgaactggtt gaaatggatg
      361 gcgaaccaga tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc
      421 tggtaaacag cctcaaaggc gtatcgggta ggtgactgcg acgagatcga ccagatattg
      481 cagtcaggta ttactacaaa ggcgttttgt ggagtgttgg ctattttgcc agtagctgcg
      541 gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca cctggttagg
      601 tggaaaacca cgccttatat ccctgccctg aaggacgggg ttttacggcg caccggataa
      661 gcctggagat tatctacacc agcatggaca tcattgtact atgccccgta catgccccat
      721 cacatcctca acatcaaagt attagagcct tgcaaaccat tatgcgtctc agtttgtctc
      781 atctatctta cgcaaaaaaa atagaactaa tatcaagcgg cgctatattg cgtattaaat
      841 aaaaccacct caattacatt aataaatcta aacaaacatc atgcaaataa tttccatggt
      901 tatttctagg ctatttaaat
//
LOCUS       DAZOYS010000457          917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOYS010000457 DAZOYS010000000
VERSION     DAZOYS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.460"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..341)
                     /locus_tag="ACHUN9_004514"
     CDS             complement(<1..341)
                     /locus_tag="ACHUN9_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0697699.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPL"
     gene            complement(358..>917)
                     /locus_tag="ACHUN9_004515"
     CDS             complement(358..>917)
                     /locus_tag="ACHUN9_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0697700.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          264 a          189 c          161 g          303 t
ORIGIN      
        1 agtggattta gatttttcag atatggtctt atgatctttc ccttacttaa taaatagcat
       61 gtataggatt cggaaagtcc agcatctatt ttgtacctca ttatattaga taaggatgtg
      121 agaaattcct tgttatcatt cgcagccata tatgccagtc cgagttcagc ctctgcggcc
      181 ttacaggcaa aatagcttag tgaacacgct tttcgtccct ctttagtact ggctgatatt
      241 agtgcccata atattgcttt gtctgccaaa tctaactctc tctgaaattg tcttgcagta
      301 actgtactcg cagaaataga gaaaaaaata caattgttaa taactttgcc atatttttta
      361 gtccgtaact gttaatgtgc tatctggctc tgaaaatatt aactcattta atttttgcat
      421 gtcattagat gaaccagtta tacaaccctc ggaacagaat cgaggtccta aagacggacc
      481 gaaagcgttt aaacaacttt gtgttaaaaa tccatcacga ttatatgttt ctgtatttgc
      541 tgacggtact aaacgtatac cattaggttt tctattggtt gctccaggac cattaacatt
      601 ccagctccat accccttgag gtattggacc tctattttgt aagtgtgtac aaccaggatt
      661 atttttacat tgcatattat taccattatt accagaggcg acaggaatag taagcacatc
      721 ataagagtta tcatcatttt gcgtatctga aacgcatgaa agcatactta aagttatgct
      781 gtataaacag gtcgctaatc ccaacgggtc aatatcagtt atcggattca acggatactg
      841 ataaaaattc catcccccct tcagtccaat cttttcgcgc cggcgtgtac accgggtcca
      901 tctggttttg catctgc
//
LOCUS       DAZOYS010000458          917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOYS010000458 DAZOYS010000000
VERSION     DAZOYS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.461"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..102)
                     /locus_tag="ACHUN9_004516"
     CDS             complement(<1..102)
                     /locus_tag="ACHUN9_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0697701.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQ"
     gene            complement(437..>917)
                     /locus_tag="ACHUN9_004517"
     CDS             complement(437..>917)
                     /locus_tag="ACHUN9_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0697702.1"
                     /translation="TIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAF
                     DYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPY
                     DDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          288 a          147 c          141 g          341 t
ORIGIN      
        1 ctgtggaata taaacttgct catttccttg agtaagaatt ccatcttccg tgagcgttaa
       61 tccctcaaga aaatgatatg agaatttatt ttctaagtcc attagttaac ctctatgaaa
      121 aaattggttt cttgcatcct gcttaagcaa tagaaattat ggctattcat atttatgtgt
      181 cattaatgct acaggaaaat acattcgaca tatctatggt tgtgttcact aatttatctt
      241 aggaattcag tgatgtaatc tgaaatagcc ttattcaatt tttataaagt agattgttct
      301 ctaacgcatt gaaaaataat gatttatagt atgctcttaa ctgttttaag atgctgattt
      361 aagaaaacat aagaggtgct ggttatagta gttaactgca ctataaccag caaatttaac
      421 caaatgattg taatgcttag ccattatctt ctgaattatc gtttaagagt tcaatatatg
      481 cctgcgcttt actcttaaca ctatcatcct cacaattgtt aataatgtga tagaaacatt
      541 gcattgcgtt atcgatattt ttagccccaa tctgacattg acccatacga taaataactg
      601 aatagtcatc atacggggag taattgtaac ttagtttgta aaggtcatat gcttgttgat
      661 atttcttttt tggctgacat actgatgcaa aatcttttag atactcatga ttttcgaacg
      721 cataaatgca caaaaattta taataaattt ctgcgtccgt tatattaccc ttttcatggt
      781 agtcgaatgc atatgcatat attctatcaa ggatatcagg agttaaacca tgtagtaatg
      841 ccagattttc tcctttcgat aatgcgtgtt ttagttgatt tgcccattca tcactattat
      901 tgaatatttc aattgtt
//
LOCUS       DAZOYS010000459          916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOYS010000459 DAZOYS010000000
VERSION     DAZOYS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.462"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            341..>916
                     /locus_tag="ACHUN9_004518"
     CDS             341..>916
                     /locus_tag="ACHUN9_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0697703.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKE"
BASE COUNT          305 a          180 c          184 g          247 t
ORIGIN      
        1 cacaagattt aaaggttatc aaaccgtcag taaatattca gcctcagaca atcaatatca
       61 caactcagac ggtacttgat ggaaaggtta ttgatgaaag aaccacatcg catattaatc
      121 gtatgcagga agataccttg attagctcag cttatccaga ggaataaaca atgttagagc
      181 tactaatatt tattgccaca catggcaatc tattaatctt tatctagtat gacgattcaa
      241 ctatgaatag tcgaatgcta cgtgaaatct tctgaacaga taccaacatc aaaaattaga
      301 tttcacgtag ctcacgctta acagaatagg aataacaaca atgtttgaca caaagcaaat
      361 tagatactca ggtaaagatg gtatctattt tcaccttaga gataatgtgg aatcttttct
      421 tgccttaaca gctactgaac agatggacta tgactcaccg atgatggtga caactcaggc
      481 tatgcaatcg gggcaaacta tcactgataa tgttcagaga gcacctaaga ccatctctat
      541 tagcggtgtt gtagtggttg attcgacggg ttcagttcta ttaaccagac aggggcaact
      601 tgtagaaacc tttatttcta cggttgaaca atggcgagac cagaagcaaa tcatcactat
      661 tgtttgtaga gatggaatag ctatcactga tagtatcatt actcagttta aagcgagtaa
      721 agagcatacc attgctaacg gtcttcgtat tcagatgacc ttccaaggtg tagagtttcg
      781 ttcaatcgta ggtcagactg atgtttcggc tgctactgga aagaccgcta ccacgaatga
      841 tggcggtgct accagtaaaa agaacaccgg aaacactaca acgagccttg gaagccctat
      901 gctgacctgt aaagag
//
LOCUS       DAZOYS010000460          911 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOYS010000460 DAZOYS010000000
VERSION     DAZOYS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.463"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..748
                     /gene="yahL"
                     /locus_tag="ACHUN9_004519"
     CDS             <1..748
                     /gene="yahL"
                     /locus_tag="ACHUN9_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0697704.1"
                     /translation="KNNQLPEDIKSSLVSCVDIFKVLIKQYYDYPYDCRDDLVDDDKL
                     IHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVKFVFRILENQKGTVWVY
                     DQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEHHMPLLRLFPKGKECMH
                     VEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLVMLLLSVLDVKTYRDDA
                     RLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          268 a          176 c          191 g          276 t
ORIGIN      
        1 taaaaacaat cagttaccag aggatatcaa aagctccctg gtttcctgtg tcgatatatt
       61 caaggttttg attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga
      121 tgacgacaaa ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa
      181 cgcactcacc ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta
      241 ccctgatcac ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg
      301 ggtatatgac caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt
      361 taccgggctt aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat
      421 tcttcttgaa caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca
      481 tgtggaaaaa tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca
      541 ggcgcttgcg catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc
      601 actggtgatg ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt
      661 aaatgaatgg atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa
      721 gcacgttaaa atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt
      781 ccaccagaga gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat
      841 ggcattccac cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa
      901 gcaccagcag t
//
LOCUS       DAZOYS010000461          908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOYS010000461 DAZOYS010000000
VERSION     DAZOYS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.464"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..797
                     /locus_tag="ACHUN9_004520"
     CDS             <1..797
                     /locus_tag="ACHUN9_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0697705.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(840..>908)
                     /locus_tag="ACHUN9_004521"
     CDS             complement(840..>908)
                     /locus_tag="ACHUN9_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697706.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          259 a          184 c          197 g          268 t
ORIGIN      
        1 ttcatgacaa tgaaaccgag ttgatttacg tcaagaaagg ggtagcaagg tttaccatcg
       61 attcttcgtt atatgtcgcg cacgccgatg acattgtggt gatagaacgt ggcaggctgc
      121 atgcggtggc ctctgacgtt aacgatccag caacgacgtg tacctgtgcg ctgtacggct
      181 ttcagtttca gggggctgag gaaaatcagc tactgcaacc gcattcttgc ccggtaattg
      241 ccgcagggca gggaaaagaa gtcattaaaa ccttatttaa tgagctaagt gtgattttgc
      301 cgcaaagtaa aaatagccaa acatcttcgt tatgggatgc atttgcctat acattagcaa
      361 ttctttacta cgaaaacttt aaaaatgctt atcgttcgga gcaggggtat attaaaaaag
      421 atgttctgat aaaagatatt cttttctatc tgaataataa ttatcgcgaa aaaatcactt
      481 tagaacagtt atcgaaaaaa tttcgtgcca gcgtcagtta tatttgccat gaatttacca
      541 aagagtatcg tatttcccct attaactatg ttattcaacg gcgtatgacg gaagcaaaat
      601 ggtcactcac taatactgaa ttatcacagg cagagatctc ctggcgtgtg ggttatgaaa
      661 atgtcgatca ctttgccaaa ctgtttttgc gccatgtcgg ttgttcgccc agcgattacc
      721 gtaggcaatt taaaaattgt tttgcggaac aagaaatctt atctgaattt cctcaaccgg
      781 taagtcttgc cggataaatc tggcggcgca ctttgttaaa aagctgcgcc gcctgcagat
      841 tatttgttct gatagtcttt caggatctgc cgacctgcta cgtaaatcat aatttcgtct
      901 gtaccgcc
//
LOCUS       DAZOYS010000462          906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOYS010000462 DAZOYS010000000
VERSION     DAZOYS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.465"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            37..822
                     /locus_tag="ACHUN9_004522"
     CDS             37..822
                     /locus_tag="ACHUN9_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697707.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          296 a          171 c          202 g          237 t
ORIGIN      
        1 ttaccatgat tatgacaggc gagggagtgt ccgattatga atttaagaag ggtttgttgt
       61 atatgtgtat tattctcgct gcttgcagga tgtgcctctg aatcttctat tgatgaaaag
      121 aagaaaaaag ctcaagtcac acaaagtaat attaataaaa acactcccca gcaactgaca
      181 gacaaagatt tattcggtaa tgaaaccact ctggccgtat ccgaagagga tattcaagct
      241 gcgcttgatg gagatgagtt ccgcgttccc cttaattctc ctgtaatact tgttcaatcc
      301 ggcaaccgcg caccggaaac cattatgcag gaggagatgc gaaaatatta tactgtttcc
      361 acattctctg gtatcccgga caggcaaaag cctctgactt gtaacaaaaa caaagataaa
      421 aatgagaacg aggatgttgc cagtgctgag aatatgaact ggatgcaggc actgcgtttt
      481 gtggctgcaa aagggcatca gaaagcgatt attgttttcc aggatacgtt gcagacagga
      541 aaatatgact ctggattgaa atcaatagtt tggtcagatt ataaaaatca aaaattgact
      601 gacacgatgt cactgcgcta tctggtacga ttcacgctgg tggatgtagc tacaggtgag
      661 tgggctgcct ggtcgccggt gaattatgaa tataaagtgc ttccaccatt gcccgacaag
      721 aatgaagcca gtacgactga tatgacagag cagcaaatca tgcaacttaa gcaaaagacc
      781 tataaagcga tggtaaaaga tttggttaac cgctatcaat aataaattat atctgccgcc
      841 aggaatttct ggcggaaata atacaaaatt ctttgcataa gaaatttttc ttgacaaaga
      901 cagtgc
//
LOCUS       DAZOYS010000463          896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOYS010000463 DAZOYS010000000
VERSION     DAZOYS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.466"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHUN9_004523"
     CDS             complement(<1..183)
                     /locus_tag="ACHUN9_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697708.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHUN9_004524"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHUN9_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
BASE COUNT          245 a          179 c          217 g          255 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctctt
//
LOCUS       DAZOYS010000464          891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOYS010000464 DAZOYS010000000
VERSION     DAZOYS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.467"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            23..852
                     /locus_tag="ACHUN9_004525"
     CDS             join(23..350,350..852)
                     /locus_tag="ACHUN9_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
                     /protein_id="HFR0697709.1"
                     /translation="MRAENARIIKPLLPPEPATPRAGRPWAEHRKIINGMFWVLCSGA
                     PWRDLPERYGSWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIR
                     ALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFA
                     QRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASD
                     GRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN
                     "
BASE COUNT          233 a          221 c          240 g          197 t
ORIGIN      
        1 tgacagcgat gaattatcgg cactgcgggc agaaaatgct cgaatcatca agcccctgtt
       61 acctcctgaa cccgccacac cacgggccgg acgcccatgg gctgagcacc gtaaaatcat
      121 caatggcatg ttctgggtgt tatgttccgg tgcaccatgg cgcgatttac ccgaacgata
      181 tgggtcatgg aaaactgttt ataaccgctt taaccgatgg tcaaagtcag gcgtgattaa
      241 tattattttc aacaggttgc tttcgctact tgatgcaaac ggctttattg actggtctgc
      301 caccgcgctg gatggcagta atatccgggc gctgaaatgt gccgccggtg ctcaaaaaaa
      361 catcccgata tcgacggaga taatgggccg ggtcgctctc gcggcggttt tggcaccaaa
      421 atccatctgg caacagacgg aagtggcctc ccgttaaata tcgtgctaag ccccggacaa
      481 gctcatgaaa gccagttcgc gcaacgactt ctggacggga ttggcgttca gcgtcagaac
      541 ggcagcatga aacgccgtgg acatgcggta ctggctgata aagcgtattc agggcgcgca
      601 ttgcgcaacg agctgaaaaa taacggtata aaggcagtaa tcccccgaaa gtcaaatgag
      661 aaaatggcat cggatggacg tgcacagctt gatcgtgatg cgtactgcaa tcgtaatgtc
      721 gttgaacggt gctttgggcg gctgaaagaa tatcgccgca tcgccacgcg ttacgacaaa
      781 acggcgagaa attacctggc gatggtgaaa ctgggctgca tccgactctt ttatcaacgc
      841 ttacgtaatt aagggacaca gcctagcact ctgcgagtaa acttgctggt a
//
LOCUS       DAZOYS010000465          888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOYS010000465 DAZOYS010000000
VERSION     DAZOYS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.468"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            4..72
                     /gene="ykiE"
                     /locus_tag="ACHUN9_004526"
     CDS             4..72
                     /gene="ykiE"
                     /locus_tag="ACHUN9_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0697710.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            159..856
                     /locus_tag="ACHUN9_004527"
                     /pseudo
     CDS             159..856
                     /locus_tag="ACHUN9_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT          213 a          216 c          237 g          222 t
ORIGIN      
        1 aaaatgggat ggagcctctg cttctggcat gtgtcggtca gaatgactca tgatgtggtc
       61 tgctattatt gacatcctca ctgccctaaa ggatagggat ttcggtaatg ctgtcaactt
      121 actgatttag tgtatgatgg tgattttaag gtgcttgcgt ggcttccatt tccatcagat
      181 gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg caaaagcact gccggacatc
      241 agcgctatct ctgctctcat tgccgtaaaa catggcaact acagttcact tacaccgcct
      301 ctcagcccgg taagcaccag aaaatcattg atatggccat gaatggcgtc ggatgtcgcg
      361 ccagtgcacg cattatgggc gttggcctca acacggtttt acgtcactta aaaaactcag
      421 gccgcagtcg gtaacctcgc gcatacaacc gggcagtgat gtgattgtct gcgctgaaat
      481 ggacgaacag tggggctacg tcggtgctaa atcacgtcag cgctggctgt tttacgcgta
      541 tgacaggata cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc tggccacact
      601 ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatagatga cggatggctg
      661 gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc gttacactca
      721 gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc
      781 actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat
      841 aaaacactat cagtaagttg gagtcattac ctcctcccga actcaaaa
//
LOCUS       DAZOYS010000466          884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOYS010000466 DAZOYS010000000
VERSION     DAZOYS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.469"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            462..809
                     /gene="tnpB"
                     /locus_tag="ACHUN9_004528"
     CDS             462..809
                     /gene="tnpB"
                     /locus_tag="ACHUN9_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0697711.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          223 a          239 c          228 g          194 t
ORIGIN      
        1 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt
       61 agtgggaacc aaagtgtcag atatgcagaa aaatgtgact cccggcaggc gaaaaggctg
      121 ccctaattat cctcccgaat ttaaacagca gctcgttgct gcctcctgtg aacccgggat
      181 atccatctca aaacttgctc ttgaaaaaat ggcattaacg ccaatctgtt gttcaaatgg
      241 cgacaacaat ggcgcgaggg aaagctgcta ttaccttctt cagagagccc ccagctactt
      301 cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc tcgcagagga cccgaaaacc
      361 ctcagtatca gcagtgaggt aacgttccgg cacgggacgc tccgcttcaa tggcaatgtc
      421 agcgaaaagc tcctgactct gctgatacag gaactgaagc gatgatcccg ttaccttccg
      481 ggatcaaaat ttggctggtt gccggtatca ccgatatgag aaatggcttc aacggcctgg
      541 ctgcgaaagt acagacggcg ctgaaagacg atcccatgtc cggccatgtt ttcattttcc
      601 ggggccgcag cggcagtcag gttaaactgc tgtggtccac cagtgacgga ctgtgcctcc
      661 tgaccaaacg gctggagcgt gggcgcttcg cctggccgtc agcccgtgat ggcaaagtgt
      721 tccttacaca ggcgcagctg gcgatgctgc tggaaggtat cgactggcga cagcctaagc
      781 ggctgctgac ctccctgacc atgctgtaaa tctctttatc ctggttgtca cagaataagc
      841 ccggtaaaat acgggcttat gaacgacatc tcttctgacg acat
//
LOCUS       DAZOYS010000467          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOYS010000467 DAZOYS010000000
VERSION     DAZOYS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.470"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUN9_004529"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUN9_004529"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0697712.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZOYS010000468          865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOYS010000468 DAZOYS010000000
VERSION     DAZOYS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.471"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            37..837
                     /locus_tag="ACHUN9_004530"
                     /pseudo
     CDS             37..837
                     /locus_tag="ACHUN9_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          249 a          168 c          209 g          239 t
ORIGIN      
        1 atattcatga agattataat cacaagggaa tacataatga aaagaaaagt tctggcaatg
       61 ctggtcccag cgttattagt tgctggcgca gcaaatgcgg ctgaaattta taataaaaat
      121 ggcaataaac tggatctgta cggaaaagtt gatgctcgtc atactttttc cgataaccct
      181 ggcgatgacg gcgatgaaac tatcatcgag ttaggcttca aaggtgagac ccaaattaca
      241 gatcagttaa ctggttatgg tcaagcactg accaaaacaa aggctagcga cactgaaggt
      301 tctgataata cttatgtcaa acttgctttt gcaggtctga agttcggcga gatgggctcc
      361 tttgactatg gtcgtaacta tggtgtgatt tacgacgttg aagcctggac agatatgctt
      421 cctgtattcg gcggcgactc ctatacctgg actgataact tcatggcagg ccgtgcaaat
      481 ggggtagcta cttaccgcaa tagtgatttc ttcggtctgg tggaaggtct gaactttgca
      541 ttgcagtatc aaggtaacaa cgaaggcagc aatgcaggag aagatcaaga aggtactaaa
      601 aatggtcacg aagacgttcg cttccagaac ggtgatggtt ttggtctttc cacttcatat
      661 gactttgact tcggtttgag cttaggtgcc gcttactcaa actctgaccg taccaattct
      721 caagtagctc ttggcgggta tcactacaat gaatacagca aatttgctgg tggtgataca
      781 gctgaagcat ggacatttgg tgcaaaatac gacgccaaca atgtttatct tgcaatgggt
      841 gatgctgcca acttactgat ttagt
//
LOCUS       DAZOYS010000469          862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOYS010000469 DAZOYS010000000
VERSION     DAZOYS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.472"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..551
                     /locus_tag="ACHUN9_004531"
     CDS             <1..551
                     /locus_tag="ACHUN9_004531"
                     /inference="COORDINATES: protein motif:HMM:NF019114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR0697713.1"
                     /translation="NMDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNV
                     LKPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKT
                     AVRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIEDGNKQE
                     EINEDTEESTTDDTTAEDTENE"
     gene            595..>862
                     /locus_tag="ACHUN9_004532"
     CDS             595..>862
                     /locus_tag="ACHUN9_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0697714.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTD"
BASE COUNT          316 a          138 c          188 g          220 t
ORIGIN      
        1 caaatatgga tatctcatta aggtcattag cgagacagta cggatacgat gaatctacag
       61 ttcgaacttg ggtagaaaag ggaatgccga cagacactga ttcaaacgca agaagttgga
      121 ttgtagataa tgtactaaag ccgttgcgtg atactaatac aaaagaacaa attgaacaag
      181 agcgtttgaa gaagctttca gcagaacggc agttagcaga attagaatta gcagaaaaga
      241 atgggctggt ggtcagtact gaatatatag aacaggtact aacggaatat ctattccagg
      301 tcaaaacagc agtgcgtgca ataccatcaa aaacttatct tgaattattt gcacaatcag
      361 atgcaaagga tttacgcgat gtattaagaa cgcatattga taaaacatta taccaattag
      421 gctcaatgga atttgagtta cccgatgaca tggaagtaat agaggatgga aacaaacaag
      481 aagaaattaa cgaggatact gaagaaagta ctaccgacga tacaaccgcc gaagatacag
      541 aaaacgagtg agtggattag taatggtgta gttaaatttg tggacgggcc gaatatgggc
      601 cttgattggg ttccgtttag tttccaaaaa gaaccaatgg atatagctca agagcgaagt
      661 actaaaaaga ttgtacttca atcttgttcc cagcttttga aaactacagt actacaatca
      721 attgcattta acctaatggc taatgatccc tgtaactttg cttttggtag tagttctgaa
      781 tcagaagtga agaaattcaa ggatggtaaa tttcttccag ctattgaaac cagttcagta
      841 ctaaagccgt tagtaaccga ta
//
LOCUS       DAZOYS010000470          842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOYS010000470 DAZOYS010000000
VERSION     DAZOYS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.473"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(360..467)
                     /locus_tag="ACHUN9_004533"
     CDS             complement(360..467)
                     /locus_tag="ACHUN9_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0697715.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          172 a          253 c          197 g          220 t
ORIGIN      
        1 taatgacaac gtttcgcggc tattcttgag ttgttcaggt tcaagaatgg cccccgtgat
       61 gttgtcaggt gcatacctgc aacgtgcggg ggttttctct ctccagcaac caatgccacc
      121 agggataaag cccccgcaac attgcgcctc accggaaccc ttccggcttg acgctgattc
      181 tacgactgtt tttctgttcc ggaatccgtc acgtcttcac tctgtgctgc gcattccgca
      241 gaatattatc cgcttgcaac cgttgcattt ttcttacgaa aaaggccgca gagtttcacc
      301 cctgcggcct gatccgggcg caaattgcca ttacggcagc ctgacgcccg catggcacgt
      361 tacttccgct tgttcagcca gttcacgatc atactggcaa gaataccagc aatgaccgga
      421 gccgctaaat catgccagaa ggccatgccc agttctgcga gcgtcatata gccgcctgta
      481 ttgtaatgac aacgtttcgc ggctattctt gtattgtttg gatgcaagaa tggcccccgt
      541 ggtgttgtca ggtgcatacc tgcaacgtgc gggggttttc tctctccagc aaccaatgcc
      601 accagggatt aagcccccgc aacattgcgc ctcaccggaa tccttccggc ttgacgctga
      661 ttctacgact gtttttctgt tccggaatcc gtctcatctt cactctgtgc tgcgcattcc
      721 gcagaatatt atccgcttgc aaccgttgca tttttcttac gaaaaaggcc gcagagtttc
      781 actcctgcgg cctggtccgg gcgcaaattg ccattacggc agcctgacgc ccacatgaca
      841 cg
//
LOCUS       DAZOYS010000471          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOYS010000471 DAZOYS010000000
VERSION     DAZOYS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.474"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUN9_004534"
     CDS             complement(<1..228)
                     /locus_tag="ACHUN9_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0697716.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUN9_004535"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUN9_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>831)
                     /locus_tag="ACHUN9_004536"
     CDS             complement(586..>831)
                     /locus_tag="ACHUN9_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697717.1"
                     /translation="LVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAI
                     EGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          266 a          149 c          160 g          256 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca g
//
LOCUS       DAZOYS010000472          828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOYS010000472 DAZOYS010000000
VERSION     DAZOYS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.475"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHUN9_004537"
     CDS             complement(19..543)
                     /gene="ygaP"
                     /locus_tag="ACHUN9_004537"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0697718.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(553..>828)
                     /locus_tag="ACHUN9_004538"
     CDS             complement(553..>828)
                     /locus_tag="ACHUN9_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0697719.1"
                     /translation="ASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGELTRITGLSA
                     SATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          205 a          214 c          195 g          214 t
ORIGIN      
        1 cgcgtccggc gtcgcttctc aagctcgttg gttccacggc atcttatcta acaaccttgc
       61 cattccacaa aaaccgctga ttcctgcaaa cagtaacccg gcaccaacaa agccacttaa
      121 taagaagaaa ccgctattta cggtataacc cagtacaacg ccgattaata ttaaaccacc
      181 cgcagcgatc tgcacctggc gcattaacgg caagggttga gatttattta ccgctactgg
      241 caatccagct cttttccagc catcaatccc atcttcgagt aaaaatattt ctgcgggggc
      301 ggcaatcgct gctaatttat cggcgttatt actggtgcgt ttacctgcct ggcagtggaa
      361 tataatttgc tcgcgacgta atttaggtgg aagacctgac tgttccagca cggataatgg
      421 agccagatct gcttcaggaa tatgttcacg aagatattca tcagcgtcac gaatatcgat
      481 taactttgcg ccgcgtgcga ttaattcttg tgcatcatgc ggcgaaatgg ttgtcaaagc
      541 catagtgact ccttacggac aatagacatt tttcagggtg gcgataatgg catttaccgc
      601 ctcattttta atggaatata gaatgcgttg ggcatcccgt tggctgtcga taagcccttc
      661 gtcccgcata cgagcgagat gctgtgatgt cgcagaggca ctcagtccgg taatgcgcgt
      721 cagctctccc gcgctggtgc cgggggaacc gctaagcatg cacagaatca gcaatcgttt
      781 aggatggctc attgctttca ataaggccgc tgcctgttcg gcactggc
//
LOCUS       DAZOYS010000473          827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOYS010000473 DAZOYS010000000
VERSION     DAZOYS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.476"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            36..>827
                     /locus_tag="ACHUN9_004539"
     CDS             36..>827
                     /locus_tag="ACHUN9_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0697720.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEI"
BASE COUNT          267 a          179 c          149 g          232 t
ORIGIN      
        1 ataccatgga agaaaaatat aaaccggagt agtgtatggc caatctctac gacttgaaaa
       61 aattcgactt aaatcttctt gtcatatttg agtgtattta ccagcattta agcattagca
      121 aagctgctga atcactatat atcaccccat cagccgtgag ccagtcgtta caacgcttac
      181 gtgcgcaatt taatgaccct gtattcattc gctcaggaaa aggtatcgcg ccgacgacaa
      241 cagggttgaa tctgcatcac catcttgaga aaaacctcaa aggccttgaa caaacaatta
      301 atatagtcaa taagtccgaa ctaaagaaaa actttattat ctacggccct cagctcattt
      361 cttgctctaa taatagtatg ctgatccgct gcctacggca agatgcctct gtcgagattg
      421 agtgccatga tatcctcatg tcagcagaga atgctgaaga actgttggtt caccgcaaag
      481 ccgatctggt gatcacccaa atgccagtga tcagccgttc cgtcatttgc atgccattgc
      541 atacaattcg caatactcta atttgtagta ataagcatcc gcgaattact gacaatagca
      601 cttatgaaca aattatggca gaagagttca cccaattaat atccaaatca gcaggagttg
      661 atgatattca aatggaaata gacgagaagt ttatgaatcg caaaatttca tttcgtggtt
      721 cctctttgct gacaataatt aatagcattg cagttacaga tttactggga atagtcccct
      781 atgaacttta taactcccac cgtgactttc ttaatttaaa agagata
//
LOCUS       DAZOYS010000474          818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOYS010000474 DAZOYS010000000
VERSION     DAZOYS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.477"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..778
                     /locus_tag="ACHUN9_004540"
     CDS             <1..778
                     /locus_tag="ACHUN9_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR0697721.1"
                     /translation="SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAI
                     NAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLR
                     VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
                     RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLA
                     EAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT          205 a          191 c          248 g          174 t
ORIGIN      
        1 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
       61 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      121 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      181 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      241 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      301 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      361 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      421 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
      481 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
      541 ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga
      601 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
      661 gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct cgccacggga
      721 atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg aaatataggg
      781 gcaaatccag tccgttataa aaacgccggt aaatcgtt
//
LOCUS       DAZOYS010000475          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOYS010000475 DAZOYS010000000
VERSION     DAZOYS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.478"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHUN9_004541"
     CDS             complement(246..>815)
                     /locus_tag="ACHUN9_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0697722.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZOYS010000476          812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOYS010000476 DAZOYS010000000
VERSION     DAZOYS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.479"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..569)
                     /locus_tag="ACHUN9_004542"
     CDS             complement(<1..569)
                     /locus_tag="ACHUN9_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0697723.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(569..>812)
                     /locus_tag="ACHUN9_004543"
     CDS             complement(569..>812)
                     /locus_tag="ACHUN9_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR0697724.1"
                     /translation="LLSIVINVLILGFAGSVIVTGGLKLIDKTLATSQVSAAMQIPMG
                     YVYIILPLSGLVMMFYALCFINQSIQQLKQPAQEAS"
BASE COUNT          276 a          194 c          172 g          170 t
ORIGIN      
        1 gcaagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc tacagaaacg
       61 ccgccagctg tcagggaaaa cacaattaac acgtttgagg gcggtatcag taatccagta
      121 atacaagatg agacgtttac agcggtagaa aatgccggat cataaccttg tttggtctga
      181 atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc agaaatggaa
      241 ccaaacatca tattcgccag gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc
      301 attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat cagcgttccg
      361 gcaaagatga aaaaggggat tgccaatagg gcaaagttat ccagtccatt agccagtcgc
      421 tgcgaaacag tgattgccgc gatatcccag ggaaacatca aaagcattga agctacagtg
      481 gcaataccga ttgagaacga aatgggcaca ccaataaaga ccagcacaaa aaaacagcca
      541 aacagtgtaa tcgcgatata actatccatt aactggcctc ctgcgcaggt tgtttcagtt
      601 gttgaatgct ttgattaata aaacacaagg cataaaacat catcaccaat ccacttaatg
      661 gcagaatgat atatacgtat cccatcggta tttgcatcgc ggcagatacc tgtgatgttg
      721 ccagcgtttt atcaattaat ttcaggccac cagtcacaat gaccgaacca gcaaaaccta
      781 aaataagaac gttaatgact atactaagaa gt
//
LOCUS       DAZOYS010000477          810 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOYS010000477 DAZOYS010000000
VERSION     DAZOYS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.480"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            205..789
                     /locus_tag="ACHUN9_004544"
     CDS             205..789
                     /locus_tag="ACHUN9_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697725.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          241 a          160 c          162 g          247 t
ORIGIN      
        1 ttgcttaatt ttgaattaag aaaatttaat ttgttcaaaa gcccttaaag tgcacaatag
       61 gcgtcctaat gaacaatggt tgtaaatttt ggtaacaaat atcattgctc attaatatct
      121 agcatctgct tataacattt gtgtcatgaa tagtcattct tcaaaccagt agtctgacta
      181 atggaatttt attcaaggat ttttatgtct aaaaaattag gttttgccct gtctggtctt
      241 atgctggcaa tggttgcagg tactgcttct gctgatatgg atggtggtca gttaaatatc
      301 agtggtctgg ttgttgataa cacctgtgaa acccgcgttg acggcggtaa taaagatggt
      361 ctgatcctgc tgcaaactgc aactgttggt gaaatcgatg ctggcgtgct gaatgacacc
      421 gttggcgcta aagccaaacc tttcagcatc accgttgact gcagcaaagc aaatccgaat
      481 ccaggtagta cagctaaaat gaccttcggt tctgttttct ttggtaatag caaaggaacg
      541 ctgaacaacg acatgtctat taacaacccg tctgacggcg tgaatatcgc tctgcacaat
      601 attgatggtt ccactatcaa acaggttcaa attaacaacc ctggcgatgt gtatactaaa
      661 gccctggatg caacgacaaa atctgctgtt tatgatttta aagcgtctta cgttcgtgcc
      721 gtcgcagacc aaacagcaac tgctggttat gtaaaaacta acactgcata caccattact
      781 tatcagtaag tattcaccaa ttaaattgat
//
LOCUS       DAZOYS010000478          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOYS010000478 DAZOYS010000000
VERSION     DAZOYS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.481"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..218)
                     /locus_tag="ACHUN9_004545"
     CDS             complement(<1..218)
                     /locus_tag="ACHUN9_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0697726.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKA"
     gene            complement(218..433)
                     /locus_tag="ACHUN9_004546"
     CDS             complement(218..433)
                     /locus_tag="ACHUN9_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0697727.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          217 a          213 c          158 g          215 t
ORIGIN      
        1 gctttgcatt ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg
       61 tgacatacag tccatacacc aatgatatct ttgtatggta tgtagctgac accttccaga
      121 ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaacagc cccaccacca
      181 atagcagctg caacagcctt gcgtaatgat ggcgacatta ttcacctctc gcagccttac
      241 gcttgtcttc tcttattttg aaatacagat ttgtcagata agtcagaagc cccagaagca
      301 gacttcccag cacaccaatc gcagcccact gtgatggact gacctgatcc aaccactgca
      361 aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt gatatttttt
      421 ccatctgata catatcccgc cccccgacag acctgtgcta tcggaaagaa aaaaggccat
      481 cagcaaaact ctgatggcct gaatcacctt taccaatatt gtatgaaaaa acacgcacga
      541 ctaattgaca ataattttca tttccattaa aaaagatcgc gtagcattct taattcatga
      601 ggaactttac ccgccagcaa tctgagtagc gaaagctgtt catccccaat gtttttgctg
      661 gcgggtcctt ttttctacgg tcctcctccc cggagggggg catttcatta tttttctaat
      721 cgttactggt gagaaggcgg aagaaaaagc cagttcttcg cccctaaata acagcatgat
      781 tccagtccat gcaaaatctg att
//
LOCUS       DAZOYS010000479          802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOYS010000479 DAZOYS010000000
VERSION     DAZOYS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.482"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            339..524
                     /locus_tag="ACHUN9_004547"
                     /pseudo
     CDS             339..524
                     /locus_tag="ACHUN9_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(624..>802)
                     /locus_tag="ACHUN9_004548"
     CDS             complement(624..>802)
                     /locus_tag="ACHUN9_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFR0697728.1"
                     /translation="SRLKTYGYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETA
                     AILYALLEKYFPGK"
BASE COUNT          225 a          192 c          184 g          201 t
ORIGIN      
        1 catggaaaac cttaaatacc agtagattac cagaaacttc tgtcgattga agacatcacc
       61 cgaagtttga attcattcga atacttttta cgaggttctt ttcgccagtc ctgtaattcc
      121 atacttaggt gttcgtctat gtcagatggg cgtctaagtt accaattctt gtctaatggc
      181 tacatacggt ggtcggttta cgcttaccgc aggacaggtg gtaactgaaa aatagtttgc
      241 tgaggcgact ggaaacagaa gggcaagatc gattccgaca gcagtgatgg tcagctacct
      301 catcgataac tttgcaacag tgcccttctt gaagcatcat gcttcatgaa gaaaaactgg
      361 cacgtcatca acgtaaacag gcgatgtata cccgaatggt agccttcccg gcggtgaaaa
      421 tgttcgaaga gtatgacttc acgttcgcca ccggagcacc gcagaagcag ctccagtcgt
      481 tacgctcact cagcttcata gagcgcaatg aaaacatagt gttacagggg acatctgaca
      541 tcacaaatcc ccgggtgggg atttgtgtat aagagacagc ccatggagga aggggatata
      601 tcgcagctct gacgactcac tccttattta cccgggaaat acttctccag caatgcatac
      661 aggatggcag cggtttcagc atccattatg ccgtcataat ttttctgccg gaaatgaagc
      721 tgaaaagcgc ggacaagcgc cttatatccg tcatccgtct ccacaccaga aatactgtat
      781 ccataggtct tcagccgtga ca
//
LOCUS       DAZOYS010000480          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOYS010000480 DAZOYS010000000
VERSION     DAZOYS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.483"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUN9_004549"
     CDS             complement(<1..741)
                     /locus_tag="ACHUN9_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0697729.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZOYS010000481          791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOYS010000481 DAZOYS010000000
VERSION     DAZOYS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.484"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..202
                     /locus_tag="ACHUN9_004550"
     CDS             <1..202
                     /locus_tag="ACHUN9_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0697730.1"
                     /translation="RPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSW
                     KIAPNERGDKKGVPGLSCFQPQ"
     gene            464..>791
                     /locus_tag="ACHUN9_004551"
     CDS             464..>791
                     /locus_tag="ACHUN9_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697731.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFI"
BASE COUNT          273 a          126 c          158 g          234 t
ORIGIN      
        1 cagaccaaaa cctgaagata tcttcaaatg tggacagacg ctcaaaaaaa ctaaacagaa
       61 atatggccat tatggattag gggtagatca gcatcaaaaa attgataatt ataccatggg
      121 gctaaattcc tggaagatag cacctaacga acgtggtgat aaaaaaggtg tcccagggct
      181 tagttgtttt caacctcaat aaaacttcat gagtgctgtg gtatctcttt ttaattttgc
      241 cagtatttca taggttttct taatcgattg tagaggcatg ttctgatgac gcctctacaa
      301 tctttaggga ctttctctgc tatgagataa gaaatttcat attatgcttt aaggctgaat
      361 gatttgtgca aaatataatt attttcccat cgaacatgag gtgtatgtca taagattaaa
      421 tgttaactat gtattcctcg cggtaaataa tatgaggtaa aagatgaaaa ttggaactgt
      481 ggcaggcact aacgacagta caacgacaat cacaacaaat gatatggtgc aagagcatgt
      541 taccaacttc actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc
      601 aagtattgcc aggacaatgc tcggtgaggt tgtggaaaaa atagatgatt ggcaaataga
      661 acggttccaa caatctattc aagatgacaa aatttcattt accatacaaa cgaatcattc
      721 agaaaaatat agtatgctat cgggcatgcg cgctcatatt ctacgtagga ataactgtta
      781 tcagtttatt g
//
LOCUS       DAZOYS010000482          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOYS010000482 DAZOYS010000000
VERSION     DAZOYS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.485"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUN9_004552"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUN9_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZOYS010000483          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOYS010000483 DAZOYS010000000
VERSION     DAZOYS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.486"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUN9_004553"
     CDS             complement(<1..458)
                     /locus_tag="ACHUN9_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0697732.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHUN9_004554"
     CDS             complement(530..742)
                     /locus_tag="ACHUN9_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697733.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZOYS010000484          787 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOYS010000484 DAZOYS010000000
VERSION     DAZOYS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.487"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
BASE COUNT          227 a          153 c          154 g          253 t
ORIGIN      
        1 ctcctgtgaa aattctcttt ccacattatt taaataaaca gagaatcaga ttaaatacct
       61 gagtataaaa tctcttctga tgtttaattg atttgaatgt tcgtaagcta tatcacttac
      121 tcaatccatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
      181 tttcagactg aagtgcgttt tcatcacccg atgagaaaga actgaagtga tgtttgccat
      241 agattgcgag atgccctgtc tcattgacta tcgcggtgca cctttatacc tgttatacca
      301 gatcaaaaat cacgcaagcc atacaacaaa acgagatttg caattcgtgt cacaaaatat
      361 gtcgatcttt ttctaagagg aagatgccat gtgaagccag acgaacactt gaggtggtct
      421 tcaaaaacta aaggatttct tttaactgtt tgttttataa aaaatttatt tagggtctaa
      481 acaagggaag ctttgcaagc taactcagtg agcgtggtga aaacaagtgt ttacccgcca
      541 tcaggctgat cataattctc accatgaaat atgtttcctg atttgtggct tgtaactggt
      601 cgcttctgaa gtcgatctag agaggcttgt tgatgttggt gttttcagga tgatgtttca
      661 cttagtttgt ttgccgtatc gcccggcgaa tagctgtgat tgaggaaggt ttaagtcgca
      721 gtgaccaaag ccatatttac ccacgaatgt agatgaaaaa gcgtctcctg cgttccccct
      781 tatctcc
//
LOCUS       DAZOYS010000485          785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOYS010000485 DAZOYS010000000
VERSION     DAZOYS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.488"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..152)
                     /locus_tag="ACHUN9_004555"
     CDS             complement(<1..152)
                     /locus_tag="ACHUN9_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0697734.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLS"
     gene            complement(200..772)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUN9_004556"
     CDS             complement(200..772)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUN9_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0697735.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          225 a          194 c          162 g          204 t
ORIGIN      
        1 ttacttaagt aaaccacccg cgtaccacca atgacaacgc ctgcataagt taatgctggt
       61 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacatgaaac tgttgtttta
      121 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      181 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      241 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      301 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta accagcgaat
      361 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      421 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
      481 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
      541 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
      601 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
      661 tagtgtcgac aatttcaccg acgaagtgaa caacaccatc ttctgccagt gcatgcccgg
      721 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
      781 cattg
//
LOCUS       DAZOYS010000486          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOYS010000486 DAZOYS010000000
VERSION     DAZOYS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.489"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..429
                     /locus_tag="ACHUN9_004557"
                     /pseudo
     CDS             1..429
                     /locus_tag="ACHUN9_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          185 a          219 c          225 g          154 t
ORIGIN      
        1 cctgtggaaa aaatacggca gtggcgataa cggtactccc ggccgatagt gaatgacctg
       61 tttgcctggc ttgaagagca ggagccgtgc tgtccgcccg acgggccatt aaataaggcg
      121 attaactata ttctgaaccg gcgcgatgaa ctgagctgct tcctgggtga cggagcggtg
      181 cccctggaca acaatatctg cgagcgtgcc attcgcccgg ttgttatggg acggaaagcg
      241 tggctgttcg ccggttcact gatggccgga aaccgggcgg cacagataat gagccttctg
      301 taaacagcaa aacgtaatgg tctggagcca catgcctggc tgacggacgt cctgacgcgt
      361 ctgccggaat ggccggagga gcgactggca gagttgctgc ctcttgaggg atttaccttc
      421 accgggtgag tgatacctgc cgtcaggtgt tcgtgaaccg ggctataacc tgcagtcggt
      481 tgttgaacgc ctgacaacag gaaatcagcc aggccagcaa ccacgcggac tgacagagaa
      541 tcagaacctg aatcagcctg aaatcatacc cggggcagta cttcatcacc agtcaccaat
      601 acccgtgtca gtacttcgcg gcctgacgcg atcacccgac tcagtacttc tgagcccgcc
      661 tccggtacca ttctctgtgg gcccgtacac aacaacagaa aagggggccc ggcccggggc
      721 gcaaccgtat tacacttccg gaaaatcgtt atacgcagaa aaatcggatc acgctgtgcc
      781 tgc
//
LOCUS       DAZOYS010000487          779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOYS010000487 DAZOYS010000000
VERSION     DAZOYS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.490"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            185..751
                     /gene="slp"
                     /locus_tag="ACHUN9_004558"
     CDS             185..751
                     /gene="slp"
                     /locus_tag="ACHUN9_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0697736.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          235 a          164 c          173 g          207 t
ORIGIN      
        1 ggattatatt atggggcgat ggatactcat gccacgcccc aaaatcaatg gaattgaact
       61 aaacaaaagg gatataaaca tcagacaggt ttacgttact atcaggcata tcgcctcaga
      121 atcagatgaa aactataaag aaatatctat tatggtttta atatttgttg ataaggatag
      181 taacatgaac atgacaaaag gtgcactcat cctcagcctt tcatttttgc ttgccgcatg
      241 tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct gatattcaaa aaagttttgt
      301 tgctgttcat aaccagccgg ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt
      361 tatcaacgtt atcaatggca aaacggatac gttgttagaa atcgctgtat taccgttgga
      421 tagctatgcg aaacctgata ttgaagccaa ctatcagggc cgactgctcg ccagacaaag
      481 cggcttcctt gacccagtga actatcgtaa tcactttgtt accatcctcg gtaccattca
      541 gggtgaacaa cctggcttta tcaataaagt cccgtataac ttcctggaag tgaatatgca
      601 gggcatccag gtgtggcatt tgagagaagt ggttaatacc acctataacc tgtgggatta
      661 cggctatggt gcattctggc cggaaccggg ctggggtgcg ccttactaca ccaatgcggt
      721 gagtcaggta acacctgagc tggtcaaata acaccaccga aagatgcaga tgctttctc
//
LOCUS       DAZOYS010000488          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOYS010000488 DAZOYS010000000
VERSION     DAZOYS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.491"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHUN9_004559"
     CDS             <1..343
                     /locus_tag="ACHUN9_004559"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0697737.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="ACHUN9_004560"
     CDS             complement(405..>775)
                     /locus_tag="ACHUN9_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR0697738.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAZOYS010000489          769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOYS010000489 DAZOYS010000000
VERSION     DAZOYS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.492"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            192..>769
                     /locus_tag="ACHUN9_004561"
     CDS             192..>769
                     /locus_tag="ACHUN9_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR0697739.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          168 a          235 c          199 g          167 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacc
//
LOCUS       DAZOYS010000490          765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOYS010000490 DAZOYS010000000
VERSION     DAZOYS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.493"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            40..756
                     /locus_tag="ACHUN9_004562"
     CDS             40..756
                     /locus_tag="ACHUN9_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0697740.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          251 a          134 c          165 g          215 t
ORIGIN      
        1 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
       61 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
      121 gtagaaatct atgctgctgc taaacaaggt gaacccgatc cagaattaaa cacatcttta
      181 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
      241 attgataaag ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt
      301 ggtcctaatg gctcaatgat tatcgccgag acattgactt caaatgttaa ccgtacgatt
      361 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
      421 agctatatgt ttgacaatgc tggtgtgatt gtatttaaag gatcagaccc tgaccatatt
      481 tttgaaattt tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac
      541 attgttattt ataccgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct
      601 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
      661 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
      721 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat ctttt
//
LOCUS       DAZOYS010000491          762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOYS010000491 DAZOYS010000000
VERSION     DAZOYS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.494"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..760
                     /locus_tag="ACHUN9_004563"
     CDS             <1..760
                     /locus_tag="ACHUN9_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0697741.1"
                     /translation="SIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLY
                     LDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYV
                     NLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKN
                     ELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKE
                     ARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          242 a          145 c          146 g          229 t
ORIGIN      
        1 ctcaattatt gtatatttaa ttgacccaaa atactttgct gatctgccga cgtcacagtt
       61 ttggtcgtac gcgacatact tcagggtatt gtcctttgaa tatctgagtg aaagtatttc
      121 cacactgctg tatctggatg ccgatgtcgt ttgtaaagga agtctgaagc ctctcacaga
      181 aattatattt aaagatgagt ttgctgcggt cattcctgac aatgttagta ctcaggcggc
      241 atgtgcaaaa cgcctcaaca ttcccgagat gaatggacgt tatttcaatg caggcgttat
      301 ctatgtcaat cttaaaaaat ggcatgaagc aaatttgaca ccgtatttac tcacgctttt
      361 acgaggggaa actaaatatg gctctcttaa atatttagat caggatgcgt tgaatatcgc
      421 atttaatatg aataatatct acctcgcgaa ggattttgat actatttata ccctgaaaaa
      481 cgaacttcat gatcgtagtc atcgaaagta tcagcaaacc attaccgata aaacagtatt
      541 gattcactat acagggataa ctaaaccatg gcatagctgg gctggttatc cgtctgcatc
      601 atactttaat atcgcgcgtg aacaatctcc ctggaagaaa tatcctctta aagaggcgcg
      661 gactgttgca gaaatgcaga aacaatataa gcatctgttt gcccatggtg agtatattaa
      721 aggcataact tcattaatta agtacaagct taagaaataa at
//
LOCUS       DAZOYS010000492          755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOYS010000492 DAZOYS010000000
VERSION     DAZOYS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.495"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /locus_tag="ACHUN9_004564"
     CDS             complement(<1..545)
                     /locus_tag="ACHUN9_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0697742.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGVPLLSCQA"
BASE COUNT          213 a          178 c          183 g          181 t
ORIGIN      
        1 catgcttgac aacttaatag gggcaccccc cgcgagccgc tatgaaccaa cagttgcaga
       61 tgttgtgcat ccagacctgc cagtgcaaac agttcagcgt cgatgatttg atcaacttgt
      121 tgcagttcag cgaaatgatt gccgccaccg atagagccaa gcgagctgcg ccacggatgc
      181 tgtgcaaatg ctgacggcag gttttcctcc agccagcttt cttcagcaac gtcatccaaa
      241 gctgataatc gcttttcaaa cttatcggcg ttgtatttgc gagcgagaat atctgtttgc
      301 catagcgcca tgccgcagcc gatatcattg ccgaccagtg ctgggtaaaa acggccaaca
      361 gagaagaacg ctgcgccaat cgggtagccg cgtccgggat gtaaatctgg catccccact
      421 acgcgctgca tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg
      481 atccacaggt catcagatgc gatggtaaat accgcatcgg ataagggacg aatatatttg
      541 cccatgtacc attccgtaaa tagactcgtc atacttcaag ttgcatgtgc tgcgactcga
      601 attattgaga gtaacaatta aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa
      661 aagaattaat cctggaaagg tctgcaaaga gtcattgata aaggatttgt catgatttac
      721 ctccttgcag tttgtggaat aagaaaacgc gcaca
//
LOCUS       DAZOYS010000493          752 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOYS010000493 DAZOYS010000000
VERSION     DAZOYS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.496"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHUN9_004565"
     CDS             complement(<1..265)
                     /locus_tag="ACHUN9_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0697743.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
     gene            322..>752
                     /locus_tag="ACHUN9_004566"
     CDS             322..>752
                     /locus_tag="ACHUN9_004566"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0697744.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          208 a          169 c          150 g          225 t
ORIGIN      
        1 ttgtgtctgg ttcttccggc cactcaatat caggtgcagt tgatgtatca acacggttca
       61 gcaacacccg atacttcttc caggcttcca gcaacgagtt ttcttcctcc gttgcgatct
      121 ccagatctac agcatcctga agtggcgcaa tatgctcact gaattcctgg atgtagaact
      181 gtgtggtgac ggtcttccag ccattcggct cctgctgtat cgaagcatac caggctattt
      241 caatatcgct atgctgcggc agcatttaac cccttgtaat tcatcgccat aattgattta
      301 attcacaaat aaaactataa tatggtgaaa ttaatgaaaa aaaacacaga tgatggggct
      361 aaaatttaca caccacttac cctaaagctt tatgactggt gggttttggg agtatcaaat
      421 cggcttgcat ggggatgtcc tacaaaggaa caccttcttc cacactttct ggaacattta
      481 ggtaacaacc atctggatat tggtgttgga actgggtttt accttactca cgtacctgag
      541 agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc atctacaagg
      601 gctggggaat caaaaattaa acataaaatt agccatgatg tttttgaacc ttatcccgcg
      661 gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg cctgcctgga
      721 aatatatcta caaaaagctg tgtaatacgc aa
//
LOCUS       DAZOYS010000494          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOYS010000494 DAZOYS010000000
VERSION     DAZOYS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.497"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            584..>751
                     /locus_tag="ACHUN9_004567"
     CDS             584..>751
                     /locus_tag="ACHUN9_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0697745.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAZOYS010000495          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOYS010000495 DAZOYS010000000
VERSION     DAZOYS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.498"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHUN9_004568"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHUN9_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZOYS010000496          728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOYS010000496 DAZOYS010000000
VERSION     DAZOYS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.499"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..443
                     /locus_tag="ACHUN9_004569"
     CDS             <1..443
                     /locus_tag="ACHUN9_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0697746.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(483..>728)
                     /locus_tag="ACHUN9_004570"
     CDS             complement(483..>728)
                     /locus_tag="ACHUN9_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR0697747.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          190 a          204 c          195 g          139 t
ORIGIN      
        1 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
       61 agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
      121 accgttcgat actggatcag ggctggtatg aaatgcaccg ccagcttgag tacaagcagc
      181 tctggcgtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag cgttgcgcgt
      241 actgtggtca tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga tgccaggtat
      301 gtggatatac agcgaacgcc gatgtaaatg gtgctcgtaa cattttagcg gcggggcacg
      361 ccgttcttgc ctgtggagag atggtgcagt caggccgccc gttgaagcag gaacccaccg
      421 aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg gcgtggagga
      481 tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac gcctccggca
      541 attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc cgttaccaga
      601 aatgccacca ccgccgcgtc gcgctcacgc tcacgctcat tatgcagcgc ctgatacaaa
      661 ctgccgccaa tggtactcaa cagcgccaga cctgccagca tctggatcca acttacgggc
      721 agggcagc
//
LOCUS       DAZOYS010000497          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOYS010000497 DAZOYS010000000
VERSION     DAZOYS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.500"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUN9_004571"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUN9_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0697748.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZOYS010000498          720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOYS010000498 DAZOYS010000000
VERSION     DAZOYS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.501"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>720
                     /locus_tag="ACHUN9_004572"
     CDS             <1..>720
                     /locus_tag="ACHUN9_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697749.1"
                     /translation="NAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGLYLDLLIQA
                     IDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTSSVALVTSG
                     LERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITLQLDLGGES
                     PGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGIKRYEKDNY
                     QSANFWFNDIKTDTDQNSHAKR"
BASE COUNT          244 a          135 c          156 g          185 t
ORIGIN      
        1 aatgctgtgc atatccgatt tgacagcaac tcccccgtga tggcagatgt gcaggaagaa
       61 ggcaagccgc agctgtggaa ccataaacaa aatgatgcgt taggtcttta tcttgatctc
      121 cttatacaag caatcgacac tggtacaatt aacgctgaag attggcaaaa aggtgaccga
      181 ttaaaatcgg ttgcgctgct gattgcttat ctggataaag ccaatttcta tgttatggaa
      241 gattccggag catgggaaga agatgcccga ttaaacacat cttccgttgc actggtgacc
      301 tctggactgg aacgtttgtc gaacttactc tcgaagaaag actctgtttt tgtctctgat
      361 ttactacgcg aagctaaagc caatgaactg gatgaacctc tttcaacaac gaggttgaac
      421 cacctcatag ataaaggtta tgaaagaata acgttacagc ttgatttagg gggagaatcc
      481 ccaggttatc tggaaaaaga taaacattac agagaagccg atgcggcttt acttaatgtt
      541 atttatcctg caaatcttgc aaagataaac accagaagaa aagagcaggt tctaaaaata
      601 gttaaaaaac tagcaggtcc gtatggtata aaaagatacg agaaggataa ttatcagtcg
      661 gcaaattttt ggttcaatga tattaaaacc gataccgatc aaaattcaca tgcgaaaaga
//
LOCUS       DAZOYS010000499          718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOYS010000499 DAZOYS010000000
VERSION     DAZOYS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.502"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..652)
                     /locus_tag="ACHUN9_004573"
     CDS             complement(<1..652)
                     /locus_tag="ACHUN9_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFR0697750.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRF"
BASE COUNT          292 a          157 c          101 g          168 t
ORIGIN      
        1 tgaaacgatg tataccgaaa ctggtattac tccagagaaa actcattacc agactatata
       61 aagtgccatt aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata
      121 taataacgct acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc
      181 ccatcccaag ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag
      241 agcaattaaa actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc
      301 cttttgctgc cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa
      361 gcggcatatc tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac
      421 aactaacaaa aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca
      481 tattagcata gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg
      541 ttataatcgt taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat
      601 gaaaaacaaa cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc
      661 cctgaattaa aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZOYS010000500          717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOYS010000500 DAZOYS010000000
VERSION     DAZOYS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.503"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(87..256)
                     /gene="yacB"
                     /locus_tag="ACHUN9_004574"
                     /pseudo
     CDS             complement(87..256)
                     /gene="yacB"
                     /locus_tag="ACHUN9_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            complement(256..525)
                     /gene="yacA"
                     /locus_tag="ACHUN9_004575"
     CDS             complement(256..525)
                     /gene="yacA"
                     /locus_tag="ACHUN9_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR0697751.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
BASE COUNT          170 a          204 c          159 g          184 t
ORIGIN      
        1 tcatgtcata cctttgttac gatatttaaa aatcacaaga gattttcaca caaaataacg
       61 ttacacgcta ccttgtgtgg tccagatgtt ccttccacac gaccgttgcg gcctttatac
      121 ggctgccctg ccaggctgct ggctgaataa ccaatccgtt cgtccggttc aatggcggcc
      181 atcaggttct gctccgcaat gtactacgaa tgcgcttcct gtcctggatg gccagcatgg
      241 tccagaacat ctccatcatt tatcagcaat cttcctgctc atttcatccc gccatgcagc
      301 agcagaggat tcgaccatgt catggggaag cacatcaccg cattcgagtt gctgacggcc
      361 tgccgcaacc tggtcacgga accaagaatt gtgctgccgt tcaacggtct ggcgcataaa
      421 atcccggatt aactgagagc cattacggtc catgcttttt gcagcggcca taaaagcatc
      481 cttcagttct gcgtcgattc ttacactcat attaacctgt gccatttgcc acctcgtcat
      541 taccacgtat atacacagta tataactgtt cagatatcgt caccaggata tgccgcgcca
      601 gcggcatgta aggcggcact ccgttgttgc atatggcaga ggagtaaaac cgccgaagcc
      661 cggcgtaagc cggtacgtat tgacaggttt cacctgtcct atcactggcg ctgacag
//
LOCUS       DAZOYS010000501          699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOYS010000501 DAZOYS010000000
VERSION     DAZOYS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.504"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>699
                     /locus_tag="ACHUN9_004576"
     CDS             <1..>699
                     /locus_tag="ACHUN9_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0697752.1"
                     /translation="RFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTTLMG
                     YTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLP
                     CQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWN
                     RFLDTHYRRGWNVNV"
BASE COUNT          159 a          177 c          211 g          152 t
ORIGIN      
        1 cgttttgccg atatctttca gcagggaaac cactggctta actggctgga gaagcagccg
       61 gaaagggcag tacgtccggt ggtgattgag tcggtgacca aaatcatggc gtgcgggacc
      121 acactgatgg ggtatacaca gtggtgctgt tcatcaccgg actgttgcca tacaaaaaag
      181 gtctgcttcc ggtgtaaaag ccgctcctgc ccgcactgcg gggtgaaggc tggcgcacag
      241 tggatacagt atctgctgag cctggtcccc gactgcccgt ggcagcatat tgtgttcaca
      301 cttccctgcc agtactggtc cctggtgttc cacaaccggt ggttactgac agagatgagc
      361 cgcattgcag cggatgtgat actggaaatc tgccatcagg cagatgtgga gccggggata
      421 ttcacggtga tccacacatg ggggcgtgac cagcagtggc atccgcatat ccatttatcg
      481 acaactgccg gtggtgtgac gtcgggccac acctggaaaa atcttcattt ttacgcccgt
      541 aaggtgatga gcatgtggcg ctaccggata acacggttgc tgtcacggaa ataccctgac
      601 ctggtgatgc cggatgcgct ggcagcagaa ggaagcagta aacgggaatg gaatcgcttc
      661 ctggacacgc attaccgccg tggctggaat gtcaacgta
//
LOCUS       DAZOYS010000502          699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOYS010000502 DAZOYS010000000
VERSION     DAZOYS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.505"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..96
                     /gene="yjbL"
                     /locus_tag="ACHUN9_004577"
     CDS             <1..96
                     /gene="yjbL"
                     /locus_tag="ACHUN9_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0697753.1"
                     /translation="LRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            87..>699
                     /locus_tag="ACHUN9_004578"
     CDS             87..>699
                     /locus_tag="ACHUN9_004578"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0697754.1"
                     /translation="MFLIEQINDLKMWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFA
                     INSICNPGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKM"
BASE COUNT          225 a          106 c          144 g          224 t
ORIGIN      
        1 ctgcgtcatc gggatataat tattgcaaaa atagatttgt ttattcaacg aatcacgcat
       61 aatttatggc atgcacgtaa aaaaatgtgt ttttaattga gcaaataaat gatctcaaaa
      121 tgtgggtcaa taagtatatt gacgattgca ctgatgagga tttaaacgat cgtgacttta
      181 ttgcatcagt tgttgaccgg gctatttttc atttcgcgat taatagtata tgtaatcctg
      241 gggataataa agatgcgact cccattgaac aatgtacttt tgatgtagaa actaagaatg
      301 gccttccctc cacggttcag ctattttatg aggaatctaa ggataatgaa cctttagcga
      361 atatacattt tcaagcaata ggttctggtt ttttaacgtt tgttaatgcc tgccaggaac
      421 atgatgacaa cagcttaaaa ttatttgctt cgctgttaat ttcactttca tattctagtg
      481 cctacgcaga tttatcagaa acagtgtata ttaatgaaaa taatgagagc tacctgaaag
      541 ctcagtttga aaaattatct caacgtgata tgaagaagta cctgggagag atgaagcgtc
      601 tggctgatgg gggagaaatg aattttgatg gctatctgga taagatgtca catctggtga
      661 atgaaggaac gctcgatcct gatattttaa gcaaaatgc
//
LOCUS       DAZOYS010000503          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOYS010000503 DAZOYS010000000
VERSION     DAZOYS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.506"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHUN9_004579"
     CDS             <1..>698
                     /locus_tag="ACHUN9_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697755.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZOYS010000504          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOYS010000504 DAZOYS010000000
VERSION     DAZOYS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.507"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUN9_004580"
     CDS             <1..>695
                     /locus_tag="ACHUN9_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0697756.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZOYS010000505          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOYS010000505 DAZOYS010000000
VERSION     DAZOYS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.508"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..690
                     /gene="yfdF"
                     /locus_tag="ACHUN9_004581"
                     /pseudo
     CDS             1..690
                     /gene="yfdF"
                     /locus_tag="ACHUN9_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          258 a          120 c          135 g          177 t
ORIGIN      
        1 tgatacagac catattacta ttggtatcga caaccaagaa ccaatagaat tacctgcgaa
       61 ctataaagac aaagaactcg tccgtactat cattaatgac aacatagttg agaagactca
      121 tgatatcaat aacaaggaaa tgatcttcag cgcattaaaa gaaatatatg atggagatcc
      181 tggttttatc ttcgataaga tatcacacaa actcagacat acggtaacgg aatttgatga
      241 gagcgggaat agcgaaccaa cggacttatt tacctggtac ggtaaagata aaaaaggcaa
      301 ctctctcgct attgtaatta aaaataaaaa cggaaatgat tacttatctc tcggttacta
      361 cgatcaggac gactaccaca ttcaaagagg aattcgtatt aatggtgata gtctcaccca
      421 atattgtagt gaaaaagcca ggaatgcttc agcaatgctt cagcgtggtt tgaaagcagt
      481 aaagctatca tggcagaatc atttgcaaca ggttccgatc atcaggttgt aaacgagctc
      541 aacggggaaa gactgagaga acctaacgaa gtttttaaac gtttaggtcg agcaataaga
      601 tataattttc aagtggacga tgcaaaattt agacgcgaca atgtaaaaga aataatttct
      661 aatttattcg ctaacaaagt tgacgttgac
//
LOCUS       DAZOYS010000506          678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOYS010000506 DAZOYS010000000
VERSION     DAZOYS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.509"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..363)
                     /locus_tag="ACHUN9_004582"
     CDS             complement(<1..363)
                     /locus_tag="ACHUN9_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HFR0697757.1"
                     /translation="MGKSLGGFFGAIITAVVVAAAVYFSGGTALAAIGWGAAAGSLSL
                     VASSMLGQIGVSGYGDVADSLSRSTSPTTGLPVIYGGELPHKNGVSGGSFILTGSIVA
                     WYNVPNSDSQYLFSEQAVA"
     gene            complement(356..>678)
                     /locus_tag="ACHUN9_004583"
     CDS             complement(356..>678)
                     /locus_tag="ACHUN9_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0697758.1"
                     /translation="IIVLRLIDLINGTTQLTNRQYSSIKEGIAGLNKEGWNHTGEIVE
                     AYCKPVQATIDGDIWLDPDNPLIMAVVASGRVLGVNADHDGFELQPKPTEGTYYRVRK
                     QRDG"
BASE COUNT          183 a          167 c          124 g          204 t
ORIGIN      
        1 tgctacagcc tgttctgaga ataagtactg agaatcagaa tttggtacgt tgtaccaggc
       61 tactatgctt ccggttagga tgaaggaacc gcctgataca ccatttttat gtggtagttc
      121 tccaccgtat ataacgggta atccagtagt tggtgaagta cttctactta gtgagtctgc
      181 tacatcaccg tatccagaaa ccccgatttg acctaacata gaacttgcaa ctaatgacaa
      241 agatccggct gctgcacccc aacctattgc tgctaatgct gtacctcctg aaaagtacac
      301 agcagccgcg acgacaactg ccgtaattat ggctccaaaa aagcccccta aactcttacc
      361 catctctctg tttccttact ctgtaatatg ttccctctgt tggttttggt tgaagttcga
      421 aaccatcatg atcagcattt actccaagta cccgaccaga tgccactact gccataatta
      481 atgggttgtc aggatctaac cagatatcac cgtctatagt ggcttgtact ggcttacagt
      541 acgcttcaac aatttcccct gtatggttcc atccttcctt gtttaaacct gctatacctt
      601 ctttaatgct cgaatactgg cgattagtta actgtgtagt accattaatt aaatcaataa
      661 ggcgtagtac tataatgt
//
LOCUS       DAZOYS010000507          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOYS010000507 DAZOYS010000000
VERSION     DAZOYS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.510"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>662
                     /locus_tag="ACHUN9_004584"
     CDS             <1..>662
                     /locus_tag="ACHUN9_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697759.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 tg
//
LOCUS       DAZOYS010000508          658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOYS010000508 DAZOYS010000000
VERSION     DAZOYS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.511"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHUN9_004585"
     CDS             <1..547
                     /locus_tag="ACHUN9_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697760.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          181 a          136 c          206 g          135 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgac
//
LOCUS       DAZOYS010000509          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOYS010000509 DAZOYS010000000
VERSION     DAZOYS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.512"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUN9_004586"
     CDS             <1..194
                     /locus_tag="ACHUN9_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0697761.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="ACHUN9_004587"
     CDS             complement(195..386)
                     /locus_tag="ACHUN9_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0697762.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>649)
                     /locus_tag="ACHUN9_004588"
     CDS             complement(491..>649)
                     /locus_tag="ACHUN9_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0697763.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          147 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaa
//
LOCUS       DAZOYS010000510          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOYS010000510 DAZOYS010000000
VERSION     DAZOYS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.513"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHUN9_004589"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHUN9_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHUN9_004590"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHUN9_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZOYS010000511          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOYS010000511 DAZOYS010000000
VERSION     DAZOYS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.514"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..641)
                     /locus_tag="ACHUN9_004591"
     CDS             complement(<1..641)
                     /locus_tag="ACHUN9_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0697764.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTL"
BASE COUNT          195 a          145 c          124 g          185 t
ORIGIN      
        1 tcgagagtac ttatgatttc gtcagtacgc tcacttcttt tggcaataaa ttgttctggg
       61 gtgatacctt taggatgtat ggattcaaac ttctgaccat cattaatcca gtcgaaaagc
      121 acctcaatat cataataaag ggatttcagt agttgattac gtgtcgcgat atgaacatca
      181 gtaataatcc ctccattagt aacatagcca aataaatcgt tgcaggcagc atcaatccaa
      241 aactcaacct cgtcattttc actaacgaga gaaaggtcaa ccacccattt acctggttta
      301 tctggcggta cgccataaga tgatgcttta ttcgtgatgc cgcagatggg tgaaccattc
      361 ttatccacaa taagaccttc gccaccgata tcaacgagag cgacgagttt gtgtctaaat
      421 ttatctgctg gacgtttacc ataaactcgc atccaggcac agtcaaacag ttgaccccag
      481 tctgagccaa tggtgagttt ctggtaagcg ccgctacggc gataaacaaa aggctgcggt
      541 tcttttgtta tccatgcttc aatcgacatt tcagagacaa cagtataaac acgctgacct
      601 attttattcg acagtttgat aatattatca gtaaggtaca tagaaatcc
//
LOCUS       DAZOYS010000512          647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOYS010000512 DAZOYS010000000
VERSION     DAZOYS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.515"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>647
                     /gene="istA"
                     /locus_tag="ACHUN9_004592"
     CDS             <1..>647
                     /gene="istA"
                     /locus_tag="ACHUN9_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0697765.1"
                     /translation="NETFHTLRALNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAP
                     ALSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHK
                     GQCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLK
                     AKPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          163 a          191 c          173 g          120 t
ORIGIN      
        1 taatgaaacc ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga
       61 catgaataac cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat
      121 gctggatgca ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa
      181 agccgtgaag gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc
      241 acacgaactg gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg
      301 gcacaagggg cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac
      361 caatccgctt cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct
      421 tatcgagcag ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa
      481 agccaaaccc catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa
      541 aaaatacggc ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc
      601 ggacagacgc tttatcgaca atctgctgcg tcatcatcgg gataacg
//
LOCUS       DAZOYS010000513          643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOYS010000513 DAZOYS010000000
VERSION     DAZOYS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.517"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>643
                     /gene="fliD"
                     /locus_tag="ACHUN9_004593"
     CDS             <1..>643
                     /gene="fliD"
                     /locus_tag="ACHUN9_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0697766.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          219 a          146 c          139 g          139 t
ORIGIN      
        1 aaagtttcca ccgaagcagg tgctgccccc ggtatctata ccatcagcgt aacccaactg
       61 gcgcaggcgc aatctttacg aactgattcc ccgacaatca ttgcatcaac aaaagatgca
      121 ctgggtgatg agtcatcaga tacacgcaca ataaaaatta cccaagacgg tcgtaaagag
      181 ccgctggaaa tcaagctcaa taaagatcag acgtcattgg atgagatcag caaagccatt
      241 aacgatgccg atagtggaat ttccgctagt atcgttaaag tcaaagatgg taactatcaa
      301 ttggtattga ccgccagtga aggtctggcc aataaaatga ccatatctgt tgagggtgac
      361 agtaaattaa atgatctgct ggcttatgac agcaagacca acaccgggaa tatgaaggaa
      421 ctggttaacg cgcaaaatgc acagctaaac gttaacggta tcgatattga acgttcaagt
      481 aacaaaatta ccgatgctcc tcagggagtg actctcgatt tgaccaaaaa agtcactgac
      541 gtccgggtca cggtgacaaa aagcaacgat aaagcaaccg aagcaattaa aggctgggtg
      601 gattcatata actccctcat tgacaccttt aacaccctga caa
//
LOCUS       DAZOYS010000514          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZOYS010000514 DAZOYS010000000
VERSION     DAZOYS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.518"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHUN9_004594"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHUN9_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZOYS010000515          638 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOYS010000515 DAZOYS010000000
VERSION     DAZOYS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.519"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..253
                     /locus_tag="ACHUN9_004595"
     CDS             <1..253
                     /locus_tag="ACHUN9_004595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697767.1"
                     /translation="IPDFKCWDIHNDSFSTDMSFYVTRYLGKMPPPYGAFHYETAPDE
                     KFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
     gene            complement(399..>638)
                     /locus_tag="ACHUN9_004596"
     CDS             complement(399..>638)
                     /locus_tag="ACHUN9_004596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697768.1"
                     /translation="VRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKY
                     VTEELKGSYAVFKLCECPVRYNCQCEFNEIGVVLF"
BASE COUNT          206 a          121 c          109 g          202 t
ORIGIN      
        1 tattccagac tttaaatgct gggatattca taacgattca tttagtactg acatgagttt
       61 ctacgttaca agatatctcg gtaaaatgcc gccgccttat ggtgcatttc attatgaaac
      121 agcacctgat gagaaatttt acagggaact ggtaataacc aatgaagcat ttgatgatgg
      181 atataattac ggttcgagat atctaaacaa agaaggaact ttgtacgatg atattaaatg
      241 taagcggctt tgatactcat gattcattgt atatagctta caaagtatat taaccattgc
      301 attcaatggg tccctccata ctggcggtac tatcgagggt tttcggcagc gggtttaatc
      361 aattgtgtgt gaacaaaatg atcatacccg caccgcattt aaaaaagtac cacaccaatt
      421 tcattaaatt cacattggca attatatctt actggacact cgcataattt aaacacggcg
      481 taagagcctt taagttcttc agttacgtac ttttccataa actcgatatc atctggttca
      541 caaaaaagat gaaatccgac accaagataa ttacgcattt tgtagttaca ctgtcttata
      601 gcactaattt tatttttgtc agcatttaag cccctaac
//
LOCUS       DAZOYS010000516          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZOYS010000516 DAZOYS010000000
VERSION     DAZOYS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.520"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>637
                     /locus_tag="ACHUN9_004597"
     rRNA            <1..>637
                     /locus_tag="ACHUN9_004597"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZOYS010000517          622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOYS010000517 DAZOYS010000000
VERSION     DAZOYS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.521"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            51..>622
                     /locus_tag="ACHUN9_004598"
     CDS             51..>622
                     /locus_tag="ACHUN9_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0697769.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
BASE COUNT          168 a          148 c          154 g          152 t
ORIGIN      
        1 cgataaatcc tactttttta atgcgatcca atcattttaa ggagtttaaa atggataaga
       61 agcaagtaac ggatttaagg tcggaactac tcgattcacg ttttggtgcg aagtctattt
      121 ccactatcgc agaatcaaaa cgttttccgc tgcacgaaat gcgcgacgat gtcgcattcc
      181 agattatcaa tgacgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct
      241 gccagacctg ggacgacgaa aatgtccaca aattgatgga tttatccatt aacaaaaact
      301 ggatcgacaa agaagaatat ccgcaatccg cagccatcga cctgcgttgc gtaaatatgg
      361 ttgccgatct gtggcatgcg cctgcgccga aaaatggtca ggccgttggc accaacacca
      421 ttggttcttc cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc
      481 gtatggaagc tgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt ccggtacaaa
      541 tctgctggca taaattcgcc cgctactggg atgtggagct gcgtgagatc cctatgcgcc
      601 ccggtcagtt gtttatggac cc
//
LOCUS       DAZOYS010000518          622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOYS010000518 DAZOYS010000000
VERSION     DAZOYS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.522"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            97..459
                     /locus_tag="ACHUN9_004599"
     CDS             97..459
                     /locus_tag="ACHUN9_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0697770.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          183 a          148 c          150 g          141 t
ORIGIN      
        1 tacgttacag aactattgca atgtcatcag ttgtgaacaa agcacctggt cgcgcatact
       61 aggggctata aatttatctt tatcagaagc catcacatga aagaagtcga aaaaaacgaa
      121 atcaaacgtc tcagcgatcg tctggacgcc atccgccacc agcaggccga tctgtcgctg
      181 gttgaagccg cagacaaata tgccgagctg gaaaaagaga aagccacgct ggaagcagaa
      241 attgctcgcc tgcgtgaagt tcatagccaa aaactgagta aagaagcaca gaagctgatg
      301 aagatgccgt ttcagcgcgc aattaccaaa aaagagcagg ctgatatggg caagctgaag
      361 aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg cgctgggccg tgaaatgggc
      421 ctgcaggaga tgactggatt ctctaagact gcgttttaag cgcctcagac gactggttcg
      481 atcatatcaa cccgcatctg gcttgcctta gctggtcaat gcggggactc tcctgtctac
      541 tcgcctaatt accaaacaat tcactccctt tacgtcgatt taccgctggt tgatattctg
      601 aaaatgcatt tttaccgaaa ta
//
LOCUS       DAZOYS010000519          611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOYS010000519 DAZOYS010000000
VERSION     DAZOYS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.523"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUN9_004600"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUN9_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUN9_004601"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUN9_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          206 a          119 c          121 g          165 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct g
//
LOCUS       DAZOYS010000520          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOYS010000520 DAZOYS010000000
VERSION     DAZOYS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.524"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHUN9_004602"
     CDS             <1..541
                     /locus_tag="ACHUN9_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0697771.1"
                     /translation="KKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDE
                     DLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQT
                     DLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPG
                     KDATPGNVYAQADYKIAYE"
BASE COUNT          181 a          131 c          140 g          152 t
ORIGIN      
        1 taaaaagtca cttcttgttg ctggcgtagc aatgattctt tccaataacg tatttgctga
       61 cgaaggtcac ggcattgtta aatttaaagg tgaagttatc tctgctccat gttcaattaa
      121 accaggtgac gaagacttaa ctgtaaatct gggtgaagta gcagataccg ttctgaaaag
      181 cgaccagaaa tctttggctg agcctttcac tattcacctg caagactgca tgttgagcca
      241 gggtggaacc acttattcaa aagcgaaggt tactttcacc acagcgaata ctatgacggg
      301 tcagactgac ctgctgaaaa acaccaaaga aactgaaatc ggtggtgcca caggcgttgg
      361 cgtacgtatt ctggatagcc agagcggcga agtgacttta ggtactccgg tagtaattac
      421 attcaacaat actaacagct atcaggaact gaacttcaaa gctcgtatgg aatccccggg
      481 caaagatgct accccgggca acgtttacgc tcaggctgac tataagattg cttacgagta
      541 atcacattag gccgggttcg cccggccaca tcattaacgg attaatgata agtggaacag
      601 atgt
//
LOCUS       DAZOYS010000521          600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOYS010000521 DAZOYS010000000
VERSION     DAZOYS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.525"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
BASE COUNT          163 a          121 c           98 g          218 t
ORIGIN      
        1 gttatttata cttatcgggt atcagttttg ataggggtat cagacttgat aggggtaaca
       61 gaattctgtg ggggtagttc actatgacct acccctagtt cactgtgagc taccgttaca
      121 tccaatcatc ttccgatgta acgtatcgtt actaccgatg tgtaatatcg ttacattcaa
      181 taattagtct atgtaacata tcgttactac caactttctt acagagagca tttcagataa
      241 atgaatgtaa cctctcgtta catccatttg tcacatagca ctctgtgtaa tgtctttcta
      301 tgtgtgtgcc ataatccccc tatattccct gtgtaaatca tagaataatg ctctctattc
      361 cttgtgtaat tccctactaa aaaatccaat gaaaagtagc ttaaaagttc aattcttact
      421 ttcttcactt ttacatcact ttcgcaaagc cttgtgtaat gcggcttaca gagcaccatc
      481 agaaattgtt ggctaaaaat tgttctgctt gtttctgaac gagtttctcg tttgtgcaaa
      541 ttttaaaatt tattttgttt ttatagcctt atttcgtgta atgcctggca tagcaagccc
//
LOCUS       DAZOYS010000522          599 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOYS010000522 DAZOYS010000000
VERSION     DAZOYS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.526"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            7..546
                     /locus_tag="ACHUN9_004603"
     CDS             7..546
                     /locus_tag="ACHUN9_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR0697772.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT          151 a          118 c          158 g          172 t
ORIGIN      
        1 aatcagatga actggcatta tgaaaaaaat ggtgtacgtt atgataacgt aacagaggaa
       61 gatattatcg aacgtatccg gcgtggtgaa ctgaatgcat caacactggt gtggcaacag
      121 ggcatgctgg aatggcagcc agtttcagcc acatcactgg cggaaatact gaaacaaagt
      181 gaagttcccc cagcccttcc gggtcaccgc attcccggag gcgttgtctg gacgctggca
      241 tttgcccctt tgattgggta catacttgag atgtggactg cagggctgaa cggtatgtca
      301 tttgaacggg cttatgatgc agtcagcgaa ggccagtact ggtttatctc tctgattctg
      361 aatattgctc tgggatatct ggatgaacga aaactccgta aatctggtgt tgatactgca
      421 acatttggct ggctggcatg gttgatcccg gtttatctgt ggcgtcgggc aaaaatcctc
      481 ggacaaaaac ctgcgtattt ctgggtctgg ttggttatgc tgatactggt gttatttacg
      541 gtctgatatt ctttatcgca gagtttacta aataatcagg ttattagtgt tcttaacag
//
LOCUS       DAZOYS010000523          589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOYS010000523 DAZOYS010000000
VERSION     DAZOYS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.528"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUN9_004604"
     CDS             <1..91
                     /locus_tag="ACHUN9_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697773.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>589
                     /gene="mcbR"
                     /locus_tag="ACHUN9_004605"
     CDS             289..>589
                     /gene="mcbR"
                     /locus_tag="ACHUN9_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0697774.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALA"
BASE COUNT          171 a          121 c          148 g          149 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctg
//
LOCUS       DAZOYS010000524          588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZOYS010000524 DAZOYS010000000
VERSION     DAZOYS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.529"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(1..116)
                     /locus_tag="ACHUN9_004606"
                     /pseudo
     CDS             complement(1..116)
                     /locus_tag="ACHUN9_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            360..588
                     /locus_tag="ACHUN9_004607"
                     /pseudo
     CDS             360..588
                     /locus_tag="ACHUN9_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          159 a          149 c          145 g          135 t
ORIGIN      
        1 aagccccggg tctgatacca gtgcaggtag accgctatct tgttccggta atgttctgcc
       61 gttctttacg gcatcacctc tagcagaaac atcgcgtaac tgcgccaagt atgatgaact
      121 tctcttaccg ctgcgatctg gttcaacaat atgcagatgg tacacaacct ctttaaccag
      181 cccatccgga ttattcagag ccgcttccgc aatccggaat aaaagtgctg ttttaccgtg
      241 aaccctctga aaatcagcga gcagtgtttt aaccagccga cgttcagagc gtactgacag
      301 acggtggaca atttgaataa acagttcaat cagaccgtag atgctctccc gttggcgaca
      361 tgctgggagc ggtggaacac cgcttcggca acgagcttcc ggcgtctcca gtgaagtgac
      421 tgacggataa tggttcatgc taccgggcta atgaaacacg ccagttcacc cggatgttgg
      481 gactttgttg aaccgaagaa cacggcggtg cggagtccgg agagtaacag aatagcaaag
      541 agcttcgtga aaacaataaa gtgtgactac atcagtatca tgcccaaa
//
LOCUS       DAZOYS010000525          586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOYS010000525 DAZOYS010000000
VERSION     DAZOYS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.530"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(68..>586)
                     /locus_tag="ACHUN9_004608"
     CDS             complement(68..>586)
                     /locus_tag="ACHUN9_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697775.1"
                     /translation="IKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGV
                     KFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSF
                     GFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMT
                     PFMYVLNTPPVI"
BASE COUNT          190 a          126 c           99 g          171 t
ORIGIN      
        1 ataatatggc cataaaaaac atcgaaagaa actcttttaa tttaacatgt aaacgcatgg
       61 ttaatcctca tatcacgggt ggagtgttaa gaacatacat aaatggagtc atgttttccc
      121 ttttccattt atcaagttcc tgttgccgtt ttagtccatc tctaattgca tattttaatt
      181 tttctgataa atggcattga gcatcgattt catttaaaac aactgtacac actatccctg
      241 tcagtaaaat tgctcctccg acaagaatta ctggaaagcc aaagctaaca caggcaaaag
      301 aagccaggct tccaatagcg gttgcaacac caccggctat aactgctttg gccacatcca
      361 ttgaaaaatc gccaagaaaa ttaaccagat ctcgctctga actcataata aattctatgg
      421 ttctccaggc tactgaaaac cagatacaga acttaactcc ttttattgat tcagataaag
      481 ctcctcgcca gccaatagcg agttccagaa cttgaggcgc tcgtgaagag aaaatatatt
      541 taccgagctt ttctaaaagc caggggctgg atgttgtgat ttttat
//
LOCUS       DAZOYS010000526          576 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOYS010000526 DAZOYS010000000
VERSION     DAZOYS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.531"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            3..389
                     /locus_tag="ACHUN9_004609"
     CDS             3..389
                     /locus_tag="ACHUN9_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0697776.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
     gene            517..>576
                     /locus_tag="ACHUN9_004610"
     CDS             517..>576
                     /locus_tag="ACHUN9_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0697777.1"
                     /translation="MASVSISCPSCSATDGVVRN"
BASE COUNT          153 a          120 c          156 g          147 t
ORIGIN      
        1 aaattaaagg gcaggttgtt gaacttgtac gtctgagcgg tcgcgagacg ttgcggcaac
       61 tggaagccaa gacaggtgcg acaagatatc tgatgagtgt tctcgccaga gagctggttg
      121 ccagtggcga tgtatacaac tctggttacg ggttattccc gtctgaacag gcgcgtaagg
      181 actggcaaaa cgcccgcaaa aaactctcaa gggcaaatct gaagaaacca tctgtggttg
      241 atcctgacct tatctggtca ttaccagacg gagaaatacg ccgctacgac aggcgtcaaa
      301 acataatctg tagcgagtgc cggaagagcg aagctatgca gcgtgtactg gctttctatc
      361 agggtaattt tcagaaggtg ctgttgtgag ccaaattaac aatcggaact tcgtgaagag
      421 aaagcataat ccaaaatctg aataattaag ttcagcactg tggtgatgct gccaacttac
      481 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
      541 ccctcctgtt cagctactga cggggtggtg cgtaac
//
LOCUS       DAZOYS010000527          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOYS010000527 DAZOYS010000000
VERSION     DAZOYS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.532"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>566)
                     /locus_tag="ACHUN9_004611"
     CDS             complement(<1..>566)
                     /locus_tag="ACHUN9_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0697778.1"
                     /translation="VISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSC
                     ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSL
                     VLGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVK
                     GNQGRLNKAFEEKFPLKELNNPEHDSYA"
BASE COUNT          153 a          134 c           99 g          180 t
ORIGIN      
        1 attgcgtaac tgtcatgctc tggattattt aattctttca gcggaaattt ttcctcaaag
       61 gctttattaa gccgcccctg gtttcctttt accgcgaata aataatcacc gccctgtttt
      121 tgtatcttct ctgcaatatc tttctggcat cccatcgcat cagttgtgat gatttttcct
      181 ttaatatcca gcatgttaag aagttcagga atggctgtaa tctcattgga tttctcatcc
      241 gtcttaatct gcccgagaac cagactgtgc attgttgaga acgcactaat gacatgaatc
      301 gctcccttgc ggcgactctt gtcataagag tgccggagcg tttttccatc aattgcaatg
      361 acgtctttat catctgaaga atggcagtca cgcatccagt taataaagca ctcgtgaaat
      421 tttgcaggac tgatacagga tacaactctg gcaatagtat cgtgaacagg aataccattt
      481 tcaaaatcac catattgctt caaaaaatcg agatgtgttt caccaaaatc ctctatatct
      541 tcccaaccct ctgcaccaga aatgac
//
LOCUS       DAZOYS010000528          564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOYS010000528 DAZOYS010000000
VERSION     DAZOYS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.533"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>564
                     /locus_tag="ACHUN9_004612"
     CDS             <1..>564
                     /locus_tag="ACHUN9_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR0697779.1"
                     /translation="LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRL
                     ALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAA
                     HLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMS
                     QGKRHNQALIALARRRCDVLFAMMRDGT"
BASE COUNT          107 a          189 c          157 g          111 t
ORIGIN      
        1 ctggcagccc agctctgcaa acttgcgcct cgtctgggta aacgccttgc agcagacata
       61 gctcaggcac tggccgaaca aaccgtcgtc gttcccggca cgaatgccgc tgccgtagta
      121 ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc aaagagacga ggtggcgctt
      181 gaggtagaac agcgagttct tgctcaccct ctttacccgg tcctgaccag tatgcccgga
      241 gtcggtgtca ggaccgcagc cagactcctc accgaggtcg cctgccgcgc cttcgcctct
      301 gccgcacatc tcgctgctta tgctggcctt gcgccggtaa ctcggcgatc cggctcgtca
      361 atacgcggtg agcatccctc gcgacggggt aataaagctc tcaaacgggc gttgttcctg
      421 tcggccttcg ccgcgctcag ggatccgctc tccagggctt actacacccg caaaatgagt
      481 cagggaaaac gacacaatca ggcgcttatc gccctggcga gacgacgctg cgacgttctg
      541 ttcgccatga tgcgcgacgg gact
//
LOCUS       DAZOYS010000529          561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOYS010000529 DAZOYS010000000
VERSION     DAZOYS010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.534"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>561)
                     /locus_tag="ACHUN9_004613"
     CDS             complement(<1..>561)
                     /locus_tag="ACHUN9_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR0697780.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          120 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact t
//
LOCUS       DAZOYS010000530          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOYS010000530 DAZOYS010000000
VERSION     DAZOYS010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.535"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>559
                     /locus_tag="ACHUN9_004614"
     CDS             <1..>559
                     /locus_tag="ACHUN9_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0697781.1"
                     /translation="ITQVVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAA
                     LAPLAKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFD
                     SQTGRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPCITSACCWSHAGRGFANLYK
                     ASREPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          116 a          155 c          166 g          122 t
ORIGIN      
        1 cattacccag gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca
       61 cattttctcc cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt
      121 tgccggagct gcactggccc cgctggcgaa actgctacgc catgagcttc tgacacggga
      181 tgttattcat gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc
      241 ctgctccggc tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg
      301 ttttgactcg cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg
      361 tggcggtacg ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc
      421 ctgcatcaca tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa
      481 ggccagcagg gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat
      541 tgagaaactc atccgtgag
//
LOCUS       DAZOYS010000531          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOYS010000531 DAZOYS010000000
VERSION     DAZOYS010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.536"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUN9_004615"
     CDS             <1..>557
                     /locus_tag="ACHUN9_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0697782.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZOYS010000532          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOYS010000532 DAZOYS010000000
VERSION     DAZOYS010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.537"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUN9_004616"
     CDS             <1..>557
                     /locus_tag="ACHUN9_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0697783.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZOYS010000533          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOYS010000533 DAZOYS010000000
VERSION     DAZOYS010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.538"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>557
                     /gene="feaR"
                     /locus_tag="ACHUN9_004617"
     CDS             <1..>557
                     /gene="feaR"
                     /locus_tag="ACHUN9_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0697784.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          144 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aatatat
//
LOCUS       DAZOYS010000534          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOYS010000534 DAZOYS010000000
VERSION     DAZOYS010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.539"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>553)
                     /locus_tag="ACHUN9_004618"
     CDS             complement(<1..>553)
                     /locus_tag="ACHUN9_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFR0697785.1"
                     /translation="KTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPM
                     ETGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDR
                     VALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAI
                     LVFPFFALLLIVMDSYINKRINY"
BASE COUNT          200 a           82 c          105 g          166 t
ORIGIN      
        1 ttataattaa ttcgcttatt aatataacta tccattacga ttaatagtaa cgcaaagaat
       61 ggaaacacta atatggctgc acgcgtttca gttactatga cagcacataa gctaagcatg
      121 aagttaattg tatataatgt tgttttatgt ctaaagtcgg attttaaaat gagaattgat
      181 gccagtaaag aagggaaaag gatgatgtag cctgctgttg tagcgtggga agctgataat
      241 gcaacacggt caacgtttaa atagaaatgt tgccacatgg catgcgcaaa aaaaagtaac
      301 tgcgttgcca gggatgcgaa cagaattagt ttcccaaatt ttatttttgt tctcaactca
      361 tttttagcaa aaagaaccaa gaaaataaaa gcactacaca aggcaatttt cccagtttcc
      421 attgggccac gatatgcatt ggtataaaca gagcctgaaa ctttataatg tgaataccag
      481 atgatattta tgattcccaa aagacataat gagatgaaaa ttaacaaagt aggattgtta
      541 gttattgttt ttt
//
LOCUS       DAZOYS010000535          548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOYS010000535 DAZOYS010000000
VERSION     DAZOYS010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.540"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHUN9_004619"
     CDS             <1..516
                     /locus_tag="ACHUN9_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR0697786.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          159 a          122 c          144 g          123 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgac
//
LOCUS       DAZOYS010000536          547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZOYS010000536 DAZOYS010000000
VERSION     DAZOYS010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.541"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            97..>547
                     /locus_tag="ACHUN9_004620"
     CDS             97..>547
                     /locus_tag="ACHUN9_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697787.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISV"
BASE COUNT          214 a          109 c           78 g          146 t
ORIGIN      
        1 gcattacatt tattgtgtgt agaagaaaat ccattcctgt tctaattatc caatttaaac
       61 atcttagcat aaaacaaatg atgaataagg aattttatgt caattgactt taccccaggt
      121 ataataaata catatcacgg cgatatttat aactgcacaa caaataccga taatgctaaa
      181 acgccagaca caccgaagtg gccttgcgat aattgggaag aacaacaacc cattaattcc
      241 acattctctg gagaaggata tatctctgat caatatgatt tagtgcaaca ccaacttcaa
      301 caaattaatg cgtgccacac gaacactaca tatacaaatg cagactactc aaaagttgtg
      361 gctcaacttg taagtcttat tactaacatt gaaacgataa gttcgacaca acttacgcaa
      421 cagacacaat caatacttaa tcagattaat aacattagat atgaaaaaaa caaaagtgct
      481 gaatgtcgaa tcattgttgt cgctaatcct aaacccgaca aggcaatcat aactaaaatt
      541 agcgtag
//
LOCUS       DAZOYS010000537          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOYS010000537 DAZOYS010000000
VERSION     DAZOYS010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.542"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUN9_004621"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUN9_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZOYS010000538          533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZOYS010000538 DAZOYS010000000
VERSION     DAZOYS010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.543"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>533
                     /locus_tag="ACHUN9_004622"
     CDS             <1..>533
                     /locus_tag="ACHUN9_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697788.1"
                     /translation="DGGFVLHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTI
                     DRWGYGVTSALSPYHENRIALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQS
                     AIMNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPV
                     SCLAHYQQSSEAEKIAQ"
BASE COUNT          155 a          103 c          135 g          140 t
ORIGIN      
        1 cgacggtggt tttgtattgc atagcggcgg cctgaccttc agtaatgaca gttttagcga
       61 ctccgataca ctggcggtag ttcaggctcc aggtgctcaa ggagcgcgaa taaattatgg
      121 caacagtact atcgatcgat ggggttatgg tgtcaccagc gctctttctc cttatcatga
      181 aaaccgtatc gcgctggata tcaacgatct tgagaacgat gttgaattaa aaagtaccag
      241 tgcagtagct gtaccgcgtc agggttcagt cgtctttgct gattttgaaa ccgtgcaagg
      301 gcaatcagcc attatgaaca tcacacgaag tgatggtaaa aatattccat ttgctgcaga
      361 tatttatgat gagcaaggca atgtcattgg taatgttgga cagggtggac aagcatttgt
      421 tcgtggcatt gagcagcagg gaaatatcag cattaaatgg ctcgaagaaa gtaaacccgt
      481 aagttgtctt gcgcattatc aacaaagttc agaagcagaa aaaatagcgc aat
//
LOCUS       DAZOYS010000539          533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZOYS010000539 DAZOYS010000000
VERSION     DAZOYS010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.544"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>533
                     /locus_tag="ACHUN9_004623"
     rRNA            <1..>533
                     /locus_tag="ACHUN9_004623"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          131 a          126 c          162 g          114 t
ORIGIN      
        1 gaactttcca gagatggatt ggtgccttcg ggaactgtga gacaggtgct gcatggctgt
       61 cgtcagctcg tgttgtgaaa tgttgggtta agtcccgcaa cgagcgcaac ccttatcctt
      121 tgttgccagc ggtccggccg ggaactcaaa ggagactgcc agtgataaac tggaggaagg
      181 tggggatgac gtcaagtcat catggccctt acgaccaggg ctacacacgt gctacaatgg
      241 cgcatacaaa gagaagcgac ctcgcgagag caagcggacc tcataaagtg cgtcgtagtc
      301 cggattggag tctgcaactc gactccatga agtcggaatc gctagtaatc gtggatcaga
      361 atgccacggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgggagtgg
      421 gttgcaaaag aagtaggtag cttaaccttc gggagggcgc ttaccacttt gtgattcatg
      481 actggggtga agtcgtaaca aggtaaccgt aggggaacct gcggttggat cac
//
LOCUS       DAZOYS010000540          530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZOYS010000540 DAZOYS010000000
VERSION     DAZOYS010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.545"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>530
                     /locus_tag="ACHUN9_004624"
     CDS             <1..>530
                     /locus_tag="ACHUN9_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0697789.1"
                     /translation="NPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPMGSLG
                     PGWKMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDE
                     GHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVL
                     TGLVDRFGRTQTFHRE"
BASE COUNT           96 a          172 c          168 g           94 t
ORIGIN      
        1 tcaatcccct gctcggtgca aaggtccttc ccggtgaaac cgacatcgcc ctgcccgccc
       61 cgctgccgtt catcctctcc cgcacctaca gcagttaccg gacaaaaacg cccgcgccga
      121 tggggagcct cggccccggc tggaaaatgc ctgcggatat ccgcttacag ctgcgcgata
      181 acacactgat actcagtgat aacggcggca gaagcctgta ttttgagcac ctgtttcccg
      241 gtgaggacgg ttacagccgc agcgaatcac tctggctggt gcgcggcggc gtggcgaaac
      301 tggatgaagg tcaccggctg gccgcactct ggcaggcgct gccggaagaa ctccgcttaa
      361 gtccgcatcg ttatctggcg acaaacagtc cgcaggggcc gtggtggctg ctcggctggt
      421 gtgagcgggt gccggaagcg gatgaggtgc tgcctgcgcc gctgccgccg taccgggtac
      481 tgaccgggct ggtggaccgc ttcgggcgca cacagacgtt ccaccgcgaa
//
LOCUS       DAZOYS010000541          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZOYS010000541 DAZOYS010000000
VERSION     DAZOYS010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.546"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(74..472)
                     /locus_tag="ACHUN9_004625"
                     /pseudo
     CDS             complement(74..472)
                     /locus_tag="ACHUN9_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          109 a          140 c          123 g          151 t
ORIGIN      
        1 tggagccaga aaaatggcag cgttacgtca ttgaactgtc cttgacccac gatattcaga
       61 cagtcgttgt atcttaagtt gacattaccc gctgacgtcg atattccctt ggtgagagat
      121 aacccaacgc actatgcgga tggttttcat tgtaatgctc gatcgccact gcaaggttat
      181 gcaatgccgt tcttggtttc ggcatgaacg cgatgcagtc ttccttcatc gttttcacga
      241 accgttctgc tattccatta ctctgagggc tactgactgc cgtcgtgcag ggctccagat
      301 tcaactctct ggcgaactgc cgtgtttcat gagccgtata agccgaaccg ttatcggcca
      361 ctggattggc cgttccggtc ctttatcgcc atatcgctga catgacatcc tgcaccgttg
      421 ctttgtcata atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa
      481 cgtaacgctg tcatcgggag aatcgtcaat gtggcattcg gtt
//
LOCUS       DAZOYS010000542          519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZOYS010000542 DAZOYS010000000
VERSION     DAZOYS010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.547"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>519)
                     /locus_tag="ACHUN9_004626"
     CDS             complement(<1..>519)
                     /locus_tag="ACHUN9_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697790.1"
                     /translation="LGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIGV
                     LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGSL
                     LMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNTV
                     QDLMNTIINMTA"
BASE COUNT          142 a          140 c          132 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgagga
//
LOCUS       DAZOYS010000543          501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZOYS010000543 DAZOYS010000000
VERSION     DAZOYS010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.548"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            76..480
                     /gene="stpA"
                     /locus_tag="ACHUN9_004627"
     CDS             76..480
                     /gene="stpA"
                     /locus_tag="ACHUN9_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0697791.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          149 a          107 c          123 g          122 t
ORIGIN      
        1 gaaatataat aagtgatcgc ttacactacg cgacgaaata cttttttgtt ttggcgttaa
       61 aaggttttct ttattatgtc cgtaatgtta caaagtttaa ataacattcg caccctccgt
      121 gcgatggctc gcgaattctc cattgacgtt cttgaagaaa tgctcgaaaa attcagggtt
      181 gtcactaaag aaagacgtga agaagaagaa cagcagcagc gtgaactggc tgagcgtcag
      241 gaaaaaatta gcacctggct ggagctgatg aaggctgacg gaattaaccc ggaagagtta
      301 ttgggtaata gctctgctgc tgcaccacgc gctggtaaaa aacgccagcc gcgtccggcg
      361 aaatataaat tcaccgatgt taacggtgaa actaaaacct ggaccggtca gggccgtaca
      421 ccgaagccaa ttgctcaggc gctggcagaa ggtaaatctc tcgacgattt cctgatctaa
      481 ttactgaggc cggatgtcgc t
//
LOCUS       DAZOYS010000544          497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZOYS010000544 DAZOYS010000000
VERSION     DAZOYS010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.549"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            47..>497
                     /locus_tag="ACHUN9_004628"
     CDS             47..>497
                     /locus_tag="ACHUN9_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR0697792.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          119 a          119 c          130 g          129 t
ORIGIN      
        1 gggtgagctg ttgctacacc ccggtatttc caccacggag gccagcatga acaacaataa
       61 cacattgtac gtcgggttag atgttcacaa agaatcgatt actgtcgctt atgctatcaa
      121 ttcagaacct gttgaactga tgggtaaaat tggcacatca cctactgata ttcagaatct
      181 ttgtaaacgt ctcaggtcaa agtcatcgca ggtcagtatc gtttatgaag cggggccctg
      241 tggttatgga ctttatcgtc ggctggtgaa atccggtttt gactgcatgg tctgcgcccc
      301 ctcacttatc ccgaaaaaac cgggggagcg ggttaaaacc gatcgccgtg atgccatcag
      361 acttgtgcgt tcactgcgtg caggagatct ttctgctgtc tacgtacccg gcattgaaga
      421 tgaggcattc cgggatttgg cccgggcatg ggcatctgcc cgcgatgatt tacggcatgc
      481 aaggcaacgt ctgaaat
//
LOCUS       DAZOYS010000545          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZOYS010000545 DAZOYS010000000
VERSION     DAZOYS010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.550"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUN9_004629"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUN9_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0697793.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZOYS010000546          486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZOYS010000546 DAZOYS010000000
VERSION     DAZOYS010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.551"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>486
                     /locus_tag="ACHUN9_004630"
     CDS             <1..>486
                     /locus_tag="ACHUN9_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0697794.1"
                     /translation="VDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAAC
                     GDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNV
                     TNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLILK
                     GS"
BASE COUNT          159 a           90 c           98 g          139 t
ORIGIN      
        1 tgttgattac aaaccccatc aagatgatta ttcttcagct gaaaaatttc tcactcactt
       61 caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg
      121 cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc
      181 aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg
      241 gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc
      301 taatgtaact aacgacaaaa taagaatcat gagcgaaagc aaagaggata ttaaacacta
      361 ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt
      421 tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat tgatattaaa
      481 aggatc
//
LOCUS       DAZOYS010000547          483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZOYS010000547 DAZOYS010000000
VERSION     DAZOYS010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.552"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..482)
                     /locus_tag="ACHUN9_004631"
     CDS             complement(<1..482)
                     /locus_tag="ACHUN9_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697795.1"
                     /translation="MFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGKIYEN
                     AWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPAS
                     NTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDA
                     "
BASE COUNT          132 a          120 c          100 g          131 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 ata
//
LOCUS       DAZOYS010000548          482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZOYS010000548 DAZOYS010000000
VERSION     DAZOYS010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.553"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..394
                     /locus_tag="ACHUN9_004632"
     CDS             <1..394
                     /locus_tag="ACHUN9_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFR0697796.1"
                     /translation="MCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIK
                     CPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSY
                     LGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            396..>482
                     /locus_tag="ACHUN9_004633"
     CDS             396..>482
                     /locus_tag="ACHUN9_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR0697797.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFL"
BASE COUNT          189 a           58 c           97 g          138 t
ORIGIN      
        1 aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa tatgtaagaa
       61 gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata gtgtagactt
      121 gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca ctcacggttt
      181 ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg atagtctaaa
      241 tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa aagaatacag
      301 tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag ctaaagggaa
      361 tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa tgttgttatt
      421 tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat aatatatttt
      481 ta
//
LOCUS       DAZOYS010000549          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZOYS010000549 DAZOYS010000000
VERSION     DAZOYS010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.554"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>475
                     /locus_tag="ACHUN9_004634"
     CDS             <1..>475
                     /locus_tag="ACHUN9_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0697798.1"
                     /translation="SDMFREAIVEGRISQNPVTPTRAPKIVVTRERLKLKIYNCIREA
                     ADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRLHVRQIKTGMMIAIPLSLSLP
                     VAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKFTGIQF"
BASE COUNT          144 a          110 c          111 g          110 t
ORIGIN      
        1 tctgatatgt ttcgcgaggc cattgttgaa ggacgtatat ctcaaaatcc agtcacgcca
       61 acaagagcac cgaaaatagt agttacaaga gaacgactga aactaaagat atacaactgc
      121 atcagggagg cagcagatca acttccggca tggttcccat tagctatgga tttagccctt
      181 gtaacaggac aacgtcgcga agacataacg aatatgcggt tcagtgatat ttatgatgat
      241 cgtctccacg tcaggcaaat taagacagga atgatgattg ctatccccct gtcactcagc
      301 cttcctgtcg ctggtctacg gcttggtaca gtagttgaac gatgccgcct ggtaagccgg
      361 ggagattatc taatcagtgc cgggattaga aaaaacagcc ctgacggcag cattcacccg
      421 gatggcctga caaaaaaatt tgtcgcagcc agaaaattca caggtatcca gttca
//
LOCUS       DAZOYS010000550          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZOYS010000550 DAZOYS010000000
VERSION     DAZOYS010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.555"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(387..>469)
                     /locus_tag="ACHUN9_004635"
     CDS             complement(387..>469)
                     /locus_tag="ACHUN9_004635"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0697799.1"
                     /translation="EYYNHHRIKEKLDGMSPVQYRLKHFS"
BASE COUNT          131 a          100 c          107 g          131 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtta acggaggtaa gcgttaacgg aggtaagcgt taacggaggt aagcgtaaac
      301 cgactgccgt atgtagccat taagccagta ttggtaacgt aggtgcccac cttttcaaat
      361 gatctgcgcc ccaaatcttg gacatcctat gaaaaatgtt taagtcgata ttgaaccgga
      421 ctcatgccat cgagtttctc tttaatgcga tggtgattgt agtattcaa
//
LOCUS       DAZOYS010000551          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZOYS010000551 DAZOYS010000000
VERSION     DAZOYS010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.556"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>469
                     /locus_tag="ACHUN9_004636"
     rRNA            <1..>469
                     /locus_tag="ACHUN9_004636"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          129 a           99 c          145 g           96 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaacc
//
LOCUS       DAZOYS010000552          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZOYS010000552 DAZOYS010000000
VERSION     DAZOYS010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.557"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHUN9_004637"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHUN9_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          109 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa accctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZOYS010000553          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZOYS010000553 DAZOYS010000000
VERSION     DAZOYS010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.558"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..114)
                     /locus_tag="ACHUN9_004638"
     CDS             complement(<1..114)
                     /locus_tag="ACHUN9_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697800.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFA"
     gene            complement(370..>468)
                     /locus_tag="ACHUN9_004639"
     CDS             complement(370..>468)
                     /locus_tag="ACHUN9_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0697801.1"
                     /translation="ISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          136 a           94 c           93 g          145 t
ORIGIN      
        1 tgcaaacttt ctttttaccg tccaataatc aatatttcta ttgtgattgt taaccactgc
       61 gtgcattggc gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt tcattgatgc
      121 ctgccttgtc gtcatgacaa acaataatag atgcttattt tcctcgcagg gttctttcgc
      181 actatcaaaa aacgctcatt tacccggcgt tacagtaaac gaagcaacat tctgtggcct
      241 acgacgatca aaagctctgt gaaaatttcc cttgttgtct ttgtctgacc ttatcgcggc
      301 gatcagcaat tattcgtttt gtgatcctca acacaaaatg ctattcgtcg cctatgaggg
      361 gagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcag aagagaaaaa
      421 gttgacgtta atcaattgtt cgagatgatc aatacctgta ttcgaaat
//
LOCUS       DAZOYS010000554          464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZOYS010000554 DAZOYS010000000
VERSION     DAZOYS010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.559"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUN9_004640"
     CDS             <1..207
                     /locus_tag="ACHUN9_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697802.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            258..446
                     /locus_tag="ACHUN9_004641"
     CDS             258..446
                     /locus_tag="ACHUN9_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR0697803.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
BASE COUNT          171 a           87 c          104 g          102 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgccagctg gacaagatac tcaaagacaa aactaacgaa atcagaatgc gaaaaaatgc
      361 tctcagggga aaaagaagca ggcgtttcca gagagcagaa agtaaaactg ataaatttta
      421 attgcgagaa acttcagtcc tcgtgaattg cattgtattc aaat
//
LOCUS       DAZOYS010000555          464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZOYS010000555 DAZOYS010000000
VERSION     DAZOYS010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.560"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>464
                     /locus_tag="ACHUN9_004642"
     CDS             <1..>464
                     /locus_tag="ACHUN9_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697804.1"
                     /translation="AARSSSKLNNDTRHEGTSYQVAYNKYLVQTATRFSVAAWRYASQ
                     DYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYW
                     GRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISIPFYWGD"
BASE COUNT          147 a           89 c           94 g          134 t
ORIGIN      
        1 gctgcccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
       61 gtcgcctata ataaatatct agtacaaaca gccactcgat ttagcgtggc cgcctggcgt
      121 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaatga taaacactat
      181 catcagagtg attatgatga tttttatgat attggcagaa aaaatagcct ttctgccaat
      241 attatgcaac ctttatccaa taatctggga aatgtatcat taagtgcgct ttggcggaat
      301 tactgggggc gaagtggaaa tgctaaagat taccaattca gttactccaa taactggcaa
      361 cacattagtt atactttctc tgccagccaa tcttatgatg aaaataataa agaagaggag
      421 cgttttaatc tgtttatctc cattcctttc tactgggggg atga
//
LOCUS       DAZOYS010000556          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZOYS010000556 DAZOYS010000000
VERSION     DAZOYS010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.561"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>463
                     /locus_tag="ACHUN9_004643"
     CDS             <1..>463
                     /locus_tag="ACHUN9_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0697805.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          119 a          108 c          149 g           87 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cac
//
LOCUS       DAZOYS010000557          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZOYS010000557 DAZOYS010000000
VERSION     DAZOYS010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.562"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>461)
                     /locus_tag="ACHUN9_004644"
     CDS             complement(<1..>461)
                     /locus_tag="ACHUN9_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NEL-type E3 ubiquitin ligase domain-containing
                     protein"
                     /protein_id="HFR0697806.1"
                     /translation="NLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQFRISL
                     SDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVSTQWTS
                     FSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT          131 a           91 c          129 g          110 t
ORIGIN      
        1 gaaagactgc tgtcgaagct ccgcagaggc actgagtttt tccagccatg cagcgacctg
       61 ttcacggaat ccggaggtat tgcgtgcaga gacggtatcg gaaaggcggt caaggaacgc
      121 ggagaatgcc cgggaattct cctcggtgga aaaagaagtc cattgtgtag aaacctcccg
      181 acgatctgct tcaggaaacc aggctgccac agtttcggga agagggggga tctgaaagtt
      241 ctgcccccct tcagtgatga ctctgatatg gcgcgaatgt aaattttgcg gcaacatctg
      301 gcgtaaaaac agtctgtttt ggtctgacag ggaaatacga aactgaggcc cgttaaaatt
      361 tggaaccgaa gtttgctgta ttaagaatag cagagtttga tctgataagg ggttatttgc
      421 aatctcaata gttatatgag gatctaaacg aaaaagattt g
//
LOCUS       DAZOYS010000558          458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZOYS010000558 DAZOYS010000000
VERSION     DAZOYS010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.563"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(139..330)
                     /locus_tag="ACHUN9_004645"
     CDS             complement(139..330)
                     /locus_tag="ACHUN9_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0697807.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            348..>458
                     /locus_tag="ACHUN9_004646"
     CDS             348..>458
                     /locus_tag="ACHUN9_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697808.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEV"
BASE COUNT          145 a           84 c          114 g          115 t
ORIGIN      
        1 caaacaggat aaacatcagt caggacaaag acgtaaaatt taagatgaaa gaatatctgc
       61 cgctttgata atcccaaaga gcaaaagcat ccgacagcga agtgccgtta tgttctgtga
      121 aagtgaagaa ggtaaccgct aacgttgtac cggtgattcg acggagagtg tggattgctg
      181 agcttctttt ttgctctgat ggtgctgcca ggcaccgacg gacgagtaga taaaacggcc
      241 aaagaagaag ataaagctga tgagcagtac gatacgggtc atgcgactgt taaatcggtg
      301 tcgttttcgc atactggttg cctgactcac aaaaggttcc ttgaagtatg tcccacgctg
      361 tggacggtac ttacattaag gcacaacagg acaaaatggt caattcttcg ttatgtaaaa
      421 aaacgtcagt ggatacatat tttaatgtta tggaagtt
//
LOCUS       DAZOYS010000559          457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZOYS010000559 DAZOYS010000000
VERSION     DAZOYS010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.564"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUN9_004647"
     CDS             <1..118
                     /locus_tag="ACHUN9_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR0697809.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNCEKLQSS"
     gene            225..>457
                     /locus_tag="ACHUN9_004648"
     CDS             225..>457
                     /locus_tag="ACHUN9_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0697810.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          161 a           93 c           99 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAZOYS010000560          453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZOYS010000560 DAZOYS010000000
VERSION     DAZOYS010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.565"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>453
                     /locus_tag="ACHUN9_004649"
     CDS             <1..>453
                     /locus_tag="ACHUN9_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0697811.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQH"
BASE COUNT          169 a           84 c           81 g          119 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atg
//
LOCUS       DAZOYS010000561          450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZOYS010000561 DAZOYS010000000
VERSION     DAZOYS010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.566"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            15..>450
                     /locus_tag="ACHUN9_004650"
     CDS             15..>450
                     /locus_tag="ACHUN9_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0697812.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRS"
BASE COUNT          176 a           77 c           92 g          105 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg
//
LOCUS       DAZOYS010000562          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZOYS010000562 DAZOYS010000000
VERSION     DAZOYS010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.567"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>449
                     /locus_tag="ACHUN9_004651"
     CDS             <1..>449
                     /locus_tag="ACHUN9_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR0697813.1"
                     /translation="RLIKNACLKLYNLAHTATNSHLKPTGPDNSDVLLFKKLFSPSKL
                     MTIIGDEIPLISEKQSLSKVLLNDENNELSDGTNFWDKNRQLTTDEIACYLQKIAANA
                     KNTQVNYPTGLYVPYSTRTHLEDALNENIKSDPSWPNEVQLFPINTG"
BASE COUNT          152 a          104 c           69 g          124 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcg
//
LOCUS       DAZOYS010000563          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZOYS010000563 DAZOYS010000000
VERSION     DAZOYS010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.568"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUN9_004652"
     tRNA            54..130
                     /locus_tag="ACHUN9_004652"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUN9_004653"
     tRNA            173..248
                     /locus_tag="ACHUN9_004653"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          127 a           93 c          109 g          119 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcg
//
LOCUS       DAZOYS010000564          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZOYS010000564 DAZOYS010000000
VERSION     DAZOYS010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.569"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..438
                     /locus_tag="ACHUN9_004654"
                     /pseudo
     CDS             1..438
                     /locus_tag="ACHUN9_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          108 a          116 c          141 g           73 t
ORIGIN      
        1 cacacgtagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc
       61 gtcacgcaga gtcagtttga cgccgtgggc aggctcagga cacagacgga tgccgcaggc
      121 cggacaacag aatacagccc ggatgtggtg acgggtctca tcacgcgcat caccacgccg
      181 gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc agccaccggg
      241 cctgacgggc tggaaatacg ccgggaatat gatgaatggg gcaacctgct gaatgaagag
      301 aacccgcatc agctgcagca gcttattcgt ttgccggggc agcagtatga tgaggagtcc
      361 ggcctgtatt ataaccgcca ccgctattat gacccgctgc aggggcggta tatcactcag
      421 gatccgattg ggctgaag
//
LOCUS       DAZOYS010000565          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZOYS010000565 DAZOYS010000000
VERSION     DAZOYS010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.570"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>435
                     /locus_tag="ACHUN9_004655"
     CDS             <1..>435
                     /locus_tag="ACHUN9_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0697814.1"
                     /translation="EFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALANIEKYPDY
                     IFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESLIRQIM
                     HGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIAS"
BASE COUNT          140 a           87 c           88 g          120 t
ORIGIN      
        1 gagttcagga ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta
       61 agagaaggac gcagaaaagc aataagaaca tttgccaccg cccttgcaaa cattgaaaaa
      121 tatcctgatt acatttttgg cgctagtcag taccaacttt ttcactggat aaaaaaagat
      181 tatccctatt tcttcgaaaa gttaaaaaaa caaatcgccg caggccgctt tgagttacaa
      241 ggttgcttct gggtcgaatg cgatttaaat ctggtaagtg gtgaatcgct gataaggcaa
      301 atcatgcatg ggacacgatt cacactgcag aatttttcaa aaaagataaa ttatgtctgg
      361 caaccggatg tctttggatt ccccgcaacg cttccacaaa ttctcaaaaa atcaggcatt
      421 aactatattg catcg
//
LOCUS       DAZOYS010000566          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZOYS010000566 DAZOYS010000000
VERSION     DAZOYS010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.571"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>435
                     /locus_tag="ACHUN9_004656"
     CDS             <1..>435
                     /locus_tag="ACHUN9_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0697815.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcg
//
LOCUS       DAZOYS010000567          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZOYS010000567 DAZOYS010000000
VERSION     DAZOYS010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.572"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..397)
                     /locus_tag="ACHUN9_004657"
     CDS             complement(<1..397)
                     /locus_tag="ACHUN9_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0697816.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          108 a          120 c           77 g          127 t
ORIGIN      
        1 ccaacctgga ccatgctaaa ttcacaccgt gctgcgcggc ctgattaaat agtgcatacg
       61 ctttggcata atccacccgc acctgctcac cgtcgtagta gatacccccc agcgctaacg
      121 gagcttctct gtcaccttgt ttagccgcta actcgaacca tttaatcgct tcctgaacat
      181 caggtttggt atcataacga ggctgataat attcccagcc taacaggctc tgcgcgtcga
      241 tatctcccga ttcagccatt ttatgcagtg tatttataac atcgggagca ggtttaccac
      301 cgtatatgct gtcatacaat tcatgcgctt tctcgctggc caccacgctt ggtgaaacta
      361 tcagcattac agaaaataat attcttttag ttaacatgta tacctttatg tcctcactgt
      421 catatatttc ca
//
LOCUS       DAZOYS010000568          431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZOYS010000568 DAZOYS010000000
VERSION     DAZOYS010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.573"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>431
                     /locus_tag="ACHUN9_004658"
     CDS             <1..>431
                     /locus_tag="ACHUN9_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0697817.1"
                     /translation="VKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRLH
                     IVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFA
                     TAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIR"
BASE COUNT          147 a           75 c          107 g          102 t
ORIGIN      
        1 gtaaaaggaa accaggggcg gctaaataaa gcctttgaga aaaaatttcc gctgacagaa
       61 ttaaataatc cagcgcatga cagttccgca atgagtgaaa agagtcacgg cagagaagaa
      121 atccgtcttc atattgtttg cgatgtccct gatgaactta ttgatttcac gtttgaatgg
      181 aaagggctga aaaaattatg cgtggcagtc tcctttcgct caataatagc agagcaaaag
      241 aaagagcccg aaatgatggt cagatattat atcagttctg ctgatttaac cgctgagaag
      301 tttgcgacag cgattcgaaa tcactggcac gtggagaata atctgcactg gcgtctggac
      361 gtggtaatga atgaagacga ctgcaaaata agaagaggaa atgcagcaga attattttca
      421 gggatacggc a
//
LOCUS       DAZOYS010000569          429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZOYS010000569 DAZOYS010000000
VERSION     DAZOYS010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.574"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>429
                     /locus_tag="ACHUN9_004659"
     CDS             <1..>429
                     /locus_tag="ACHUN9_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0697818.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLST"
BASE COUNT          123 a           92 c          103 g          111 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcac
//
LOCUS       DAZOYS010000570          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZOYS010000570 DAZOYS010000000
VERSION     DAZOYS010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.575"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUN9_004660"
     tRNA            41..116
                     /locus_tag="ACHUN9_004660"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZOYS010000571          411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZOYS010000571 DAZOYS010000000
VERSION     DAZOYS010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.576"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHUN9_004661"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHUN9_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          114 a           84 c           95 g          118 t
ORIGIN      
        1 ttccgctggc agggcctgga tggctcagaa attctgatgc ataactttcc tgaagacact
       61 tatgatagcc gagcacgtgc tcgctcttta gaatatattg aacaaaacta taatgaaaaa
      121 gagatctgtc cctatgcgct tatggtgtat ggtgtatggt gtcggtgatg gcggcgctgg
      181 ccccggtgag gaacatatag aacgattaac acgtatacgc aatattgatg gcttacctca
      241 tgttgatttc agccgtgttg ataagttctt cacttacgcc gacgcctttc gcgaaagctt
      301 gcccattatt tcaggtgaac tttatttcga ggctcatcaa gggtgcttta catcagagtc
      361 agcaactaaa gctcataatc gaaatatgga aaataaatta catgatgctg a
//
LOCUS       DAZOYS010000572          403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZOYS010000572 DAZOYS010000000
VERSION     DAZOYS010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.577"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>403
                     /gene="istB"
                     /locus_tag="ACHUN9_004662"
     CDS             <1..>403
                     /gene="istB"
                     /locus_tag="ACHUN9_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0697819.1"
                     /translation="LLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQG
                     RVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPV
                     DKWHGLMENPTTADAILDRLVHNSHRVVLQG"
BASE COUNT          120 a           87 c          116 g           80 t
ORIGIN      
        1 acctactgat aaccggtccg acaggatgcg gtaagagctg gatagccaat gcgctgggcg
       61 aacaggcatg tcgacagaaa tacagcgtcc ggtactgtag aaccgggcga ttactggaac
      121 agttggctca gggacgtgtt gatggcagtt ggctgaaata tctgaaacag ttgcaaaaaa
      181 tacaggttct aatcctggac gatctggggc tggaacagtt aagtaacgcc cagtgtaatg
      241 accttctgga gatcacagag gatcgttacg gacaaagcag cacgatagtg gtaagccagt
      301 tcccggtaga taaatggcat ggtctgatgg agaacccaac aacagcagat gccatcctgg
      361 acagactggt acataactca cacagagtgg tccttcaggg aga
//
LOCUS       DAZOYS010000573          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZOYS010000573 DAZOYS010000000
VERSION     DAZOYS010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.578"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(41..241)
                     /gene="glgS"
                     /locus_tag="ACHUN9_004663"
     CDS             complement(41..241)
                     /gene="glgS"
                     /locus_tag="ACHUN9_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0697820.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          122 a           96 c           65 g          112 t
ORIGIN      
        1 tctaatacaa acactaaaac gggccatcag gcccgttata tcagtgctct aactccagct
       61 ctcttgcctg catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat
      121 gttcttcatc catgttacca gtaacggcaa gaatatcgac cgggcgacct tctgcgtgca
      181 ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga ctatgatcca
      241 taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt
      301 attccccaat cagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca
      361 cactttttag tgaaaaataa aaaactatat taaaa
//
LOCUS       DAZOYS010000574          391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZOYS010000574 DAZOYS010000000
VERSION     DAZOYS010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.579"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(53..>391)
                     /locus_tag="ACHUN9_004664"
     CDS             complement(53..>391)
                     /locus_tag="ACHUN9_004664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697821.1"
                     /translation="LMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNHY
                     TESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPLNI
                     INVICAKEHI"
BASE COUNT          108 a           79 c           79 g          125 t
ORIGIN      
        1 gctcctacgt aaaaaatcca tcacttttcc cctgatacat caattcaagg cgttatatgt
       61 gttccttagc acatataaca tttattatgt taagtggatt ttggcctata ttttgacctg
      121 cacccgcata aacactggtt tcatcgctta cagcccttgt gagacgtggt gctgtttcta
      181 gcatctgatc cgacataaaa ccgatcattc tcgtgtttag tgaaagttta gtgagagaca
      241 ttacagaaag gctttctgtg taatgattta ataggtaaaa acgatgttga ttacgcaaga
      301 aaattccttg ctgatatttg gggattctgg attttccaga tcggggggcg ctgaaaatcg
      361 cgttaagcac ctctcgcaat ctagccataa g
//
LOCUS       DAZOYS010000575          378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZOYS010000575 DAZOYS010000000
VERSION     DAZOYS010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.580"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(72..260)
                     /locus_tag="ACHUN9_004665"
     CDS             complement(72..260)
                     /locus_tag="ACHUN9_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697822.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQCEWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT           90 a           93 c          116 g           79 t
ORIGIN      
        1 gtccagcact ggttggccgt gaacagacgt ttcagcgggc ggggagtaaa agcggacaac
       61 atgaggacgg attaaccccc ggtatcctgt agccgggtaa gggctgctgt gtcgccacgg
      121 cgctccagga tggaggccgc gaccatcgca ggtggtgggt ccatgggttt gagcatcttc
      181 atccagtccc attcacattg tgagagcttc tcagcaccaa cagagagcag aacggtatct
      241 atacttttgc gggtcaacat aagaggatga gtccttaata aaacaggtcc tcagagcata
      301 catattctgc ggagcgtgcg gcaacaacag gacgaacagg ggcggttttc aatttgcagc
      361 cgtcaggctg ccgtggtt
//
LOCUS       DAZOYS010000576          376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZOYS010000576 DAZOYS010000000
VERSION     DAZOYS010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.581"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..376
                     /locus_tag="ACHUN9_004666"
                     /pseudo
     CDS             1..376
                     /locus_tag="ACHUN9_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          128 a           73 c           79 g           96 t
ORIGIN      
        1 taaatttgat gtgcgaatct ctcaactttc agaagaaatt aacgcagcag caagattgat
       61 ttatatggtc atggtccata gctgtgactt ctatgaagag aaatacaaat catattcacc
      121 aagtattgac catctcgctt atatggctgg tgtatcaaag agaacagcaa tcagagccat
      181 tgagacactt gagaaacacg gtttaattca ggtgcaaaag aatcaggggc gtcaaagcac
      241 ttatcgtgta ttcacactgg atgataagcc ggaattactt gtaagaccta catcacagga
      301 agttgcagcc tcagtaagac aaaatgacac cactaccagt gacaaaatgt catgggggta
      361 gtgacatttt gtcacc
//
LOCUS       DAZOYS010000577          370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZOYS010000577 DAZOYS010000000
VERSION     DAZOYS010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.582"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>370)
                     /locus_tag="ACHUN9_004667"
     CDS             complement(<1..>370)
                     /locus_tag="ACHUN9_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0697823.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTY"
BASE COUNT          132 a           75 c           76 g           87 t
ORIGIN      
        1 tataggtcac cattgccagc accaatacca tcacggcata gaatggcagc ataggtttga
       61 taattttatt aatcggtaat ttagatacac tacaaccgac aaataacaca ctgcctacag
      121 gcggagtaca aataccgata cacatgttca gtaccatgac aataccaaag tgaatcgggt
      181 ccatgcccag ttcggtgaca acaggtaaaa atatcggcgt aaaaatcagt accgccggtg
      241 tcatatccat aaaagtgccg ataatcaaca ggataatatt gataataagc aggattatta
      301 ttgggttgtc agaaatagtg ttaagcaaat cgagaatcag gaagggaacg tcagcgtttg
      361 acattgccca
//
LOCUS       DAZOYS010000578          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZOYS010000578 DAZOYS010000000
VERSION     DAZOYS010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.583"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>367
                     /locus_tag="ACHUN9_004668"
     CDS             <1..>367
                     /locus_tag="ACHUN9_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0697824.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAZOYS010000579          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZOYS010000579 DAZOYS010000000
VERSION     DAZOYS010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.584"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHUN9_004669"
     CDS             <1..337
                     /locus_tag="ACHUN9_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR0697825.1"
                     /translation="KDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFG
                     NGRFGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAI
                     GQSIKNHLE"
BASE COUNT          113 a           58 c           91 g          105 t
ORIGIN      
        1 gaaggactgg ccgaaagagt tggttgccgc ttgtcatatg aaatccagcg gtaaagggta
       61 tgctagtgta tatgggcgca tgtcttggga taagccaagt ccaacaatta caactctttg
      121 ttatggtttc gggaatgggc gttttggaca tccagaacag catagagcga tttctcttag
      181 agaggctgct ttattgcaaa ctttcccgat ggattatatt tttgtagaag acaaaaacaa
      241 gtttgttata agaagtattg ggaaaatgat aggtaatgct gttcctgtgg aactaggaaa
      301 agcaattggg caatcaatca agaaccattt agaatgataa aatggcctgc tttacaggcc
      361 ttataac
//
LOCUS       DAZOYS010000580          362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZOYS010000580 DAZOYS010000000
VERSION     DAZOYS010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.585"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
BASE COUNT          129 a           54 c           71 g          108 t
ORIGIN      
        1 gtaactttga tgaccctaac aatagatcgg aattttctta gagtcaatgg tcactgtttt
       61 tgcttgcaaa gcaaatgttt tggtggttat aagttgtctt aatatagtca cgcatgcaac
      121 ttgttccatg cggagattaa agattggaat ataacgcgaa gatggaatta aattacattt
      181 gcaataatca atttattatt actcaataat taccgatatt attattgtaa attaatcatt
      241 agagtgcaaa aagaaatgta acggcaaaat aagaggacga tagctgagaa aaaagcgtgc
      301 tttcccaatc tggggcaaat cacgtctgaa tgacacctga aaacaggtgg tagcctcaag
      361 aa
//
LOCUS       DAZOYS010000581          361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZOYS010000581 DAZOYS010000000
VERSION     DAZOYS010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.586"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..244)
                     /locus_tag="ACHUN9_004670"
     CDS             complement(<1..244)
                     /locus_tag="ACHUN9_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0697826.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSIN"
BASE COUNT          112 a           72 c           76 g          101 t
ORIGIN      
        1 tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc tggcagaaag
       61 tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata atctggaatg
      121 cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata gtagaaatgg
      181 cctttgcgcc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac agcttctggt
      241 ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt atccgcaatg
      301 gaagcaaggc attacaggct taatttaaat aacaaaatcc taagcagaaa aacgactttt
      361 t
//
LOCUS       DAZOYS010000582          360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZOYS010000582 DAZOYS010000000
VERSION     DAZOYS010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.587"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(16..240)
                     /locus_tag="ACHUN9_004671"
     CDS             complement(16..240)
                     /locus_tag="ACHUN9_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0697827.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          103 a           90 c           62 g          105 t
ORIGIN      
        1 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
       61 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
      121 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
      181 aaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      241 cgaagcatac taggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      301 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
//
LOCUS       DAZOYS010000583          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZOYS010000583 DAZOYS010000000
VERSION     DAZOYS010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.588"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHUN9_004672"
     rRNA            <1..>357
                     /locus_tag="ACHUN9_004672"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZOYS010000584          356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZOYS010000584 DAZOYS010000000
VERSION     DAZOYS010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.589"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /locus_tag="ACHUN9_004673"
     CDS             complement(<1..254)
                     /locus_tag="ACHUN9_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0697828.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWA"
BASE COUNT          125 a           80 c           54 g           97 t
ORIGIN      
        1 gcccatgaaa actcaagatc gtcaccctcc agatcagcat aagaataaaa tgtaggctca
       61 tgtgtaataa tgatattgac accttcaaaa acagcctttc ttaaaacatc cattgaggca
      121 atccagcagg tcccaatttt attaataagt gtcgcctcag caccaaaaat aattttatca
      181 acagttggtt gcgaaacagc aaccattgaa tttaaaaaac aattaagatc cgcggctgtt
      241 ttaatatgat gcattcaaca tccttcacag gtttcatcag caataaaata atttcaacat
      301 ttctgggaaa cgttttgccc atgcagactc agagtgggta tcacctcccc ataaat
//
LOCUS       DAZOYS010000585          356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZOYS010000585 DAZOYS010000000
VERSION     DAZOYS010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.590"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..>356)
                     /locus_tag="ACHUN9_004674"
     CDS             complement(<1..>356)
                     /locus_tag="ACHUN9_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0697829.1"
                     /translation="NGIIVGRDRLARLRKELRLHCKQKRKFRATTNSDHNLPVTPNLL
                     NQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIVGYAMGERMTKELTGKAL
                     FMALRSQRPPAGLIHHT"
BASE COUNT           72 a           98 c           95 g           91 t
ORIGIN      
        1 gtgtggtgga ttagcccggc aggtgggcgc tggctcctga gcgccataaa cagggcttta
       61 cccgtcaact ctttcgtcat gcgctctccc atggcgtaac cgacaatttc gcacgtataa
      121 acatctttga cgccggcgag atacagccat ccttcccggg tggcaacata cgttatatct
      181 gcgacccaga cctgattagg tgctgtgggc gtaaagttct gattcagcag attcggcgta
      241 accggcagat tgtggtcaga gtttgtggtc gccctgaact tgcgtttctg cttacagtgc
      301 agcctcagct ccttacgaag acgtgccagt ctgtcacgac cgacgataat tccgtt
//
LOCUS       DAZOYS010000586          352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZOYS010000586 DAZOYS010000000
VERSION     DAZOYS010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.591"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(61..>352)
                     /locus_tag="ACHUN9_004675"
     CDS             complement(61..>352)
                     /locus_tag="ACHUN9_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR0697830.1"
                     /translation="VKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIE
                     EAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          110 a           68 c           48 g          126 t
ORIGIN      
        1 gggactatat atttcgtttt cagataagac tccttgagag attatctttt atgtaaaatc
       61 tcaaatatca cctgatgtaa tgtcaatgat aatattaagt gcgctctgaa caacatcttt
      121 cttggtgtga ttaacatctg acaaaaagaa catcagcttc tttattatgt tcttgttgtt
      181 tattatttca cgctcacaga ataaagcgtt gactgcctct tctatgacat ttctctcgta
      241 aaaaaattga gagttagcct catataaaat attggccaat ttttcaccaa aaaccatatt
      301 ataataatca ctgtgcatct tcctccccca gggttcagtc aataatttta cc
//
LOCUS       DAZOYS010000587          352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZOYS010000587 DAZOYS010000000
VERSION     DAZOYS010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.592"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(166..>352)
                     /locus_tag="ACHUN9_004676"
     CDS             complement(166..>352)
                     /locus_tag="ACHUN9_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0697831.1"
                     /translation="ATLKGFKSGELSFYEYRKNLATAGVFRWITNIHENKRYYYTFDN
                     SLLFTENIQNTTQIFPH"
BASE COUNT           93 a           70 c           82 g          107 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg ct
//
LOCUS       DAZOYS010000588          350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZOYS010000588 DAZOYS010000000
VERSION     DAZOYS010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.593"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            15..>350
                     /locus_tag="ACHUN9_004677"
     CDS             15..>350
                     /locus_tag="ACHUN9_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0697832.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGL"
BASE COUNT           74 a           88 c          100 g           88 t
ORIGIN      
        1 cggaggaaaa catcatgcaa tatgcgaaac ctgtcactct gaatgttgaa gagtgcgacc
       61 gtctctcctt tctgccttac ctgtttggca acgattttct gtatgccgag gcgtatgtgt
      121 acgcgctggc gcaaaaaatg atgccggaat atcagggcgg cttctggcac ttcatccgcc
      181 tgccggacgg tggcggttac atgatgccgg atggcgaccg tttccacatg gtgaacggtg
      241 caaactggtt tgaccgtacc gtgagtgctg atgccgcagg catcattctg acttctcttg
      301 tgattaaccg ccaattgtgg ctgtaccacg acagcgggga tgcaggactg
//
LOCUS       DAZOYS010000589          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZOYS010000589 DAZOYS010000000
VERSION     DAZOYS010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.denovo.594"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUN9_004678"
     CDS             complement(<1..304)
                     /locus_tag="ACHUN9_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0697833.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          106 a           72 c           74 g           97 t
ORIGIN      
        1 ttgaatcctc tctcggcacg gttgcaatta gcgagtgggt agtatcacca acagcgcgaa
       61 atccgcgtct gataatggat atgttctgat ataacgtgtt agtaggcaca cgcatacctt
      121 cttcttccca gactctggtg aaaaaatctt gttgtgtaac cacatcagga aaagcttcaa
      181 gtagaagtga taaacatcga ctggctggcg ttgtcagaac gacgttaaga tcggcgttat
      241 gtaccgatat taattttcgg tgctcaggcc agaactcgat attatcgtta ataatccagt
      301 acatacgtca ataaacacta aatcaatcga aatggagatc acatagttt
//
LOCUS       DAZOYS010000590         8955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.guided.159, whole genome shotgun sequence.
ACCESSION   DAZOYS010000590 DAZOYS010000000
VERSION     DAZOYS010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.guided.159"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            complement(<1..705)
                     /gene="epmA"
                     /locus_tag="ACHUN9_004679"
     CDS             complement(<1..705)
                     /gene="epmA"
                     /locus_tag="ACHUN9_004679"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0697834.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAER"
     gene            1030..2838
                     /gene="frdA"
                     /locus_tag="ACHUN9_004680"
     CDS             1030..2838
                     /gene="frdA"
                     /locus_tag="ACHUN9_004680"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0697835.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2831..3565
                     /gene="frdB"
                     /locus_tag="ACHUN9_004681"
     CDS             2831..3565
                     /gene="frdB"
                     /locus_tag="ACHUN9_004681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0697836.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3576..3971
                     /gene="frdC"
                     /locus_tag="ACHUN9_004682"
     CDS             3576..3971
                     /gene="frdC"
                     /locus_tag="ACHUN9_004682"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0697837.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3982..4341
                     /gene="frdD"
                     /locus_tag="ACHUN9_004683"
     CDS             3982..4341
                     /gene="frdD"
                     /locus_tag="ACHUN9_004683"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0697838.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4404..5537
                     /locus_tag="ACHUN9_004684"
     CDS             4404..5537
                     /locus_tag="ACHUN9_004684"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0697839.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            5626..6159
                     /gene="blc"
                     /locus_tag="ACHUN9_004685"
     CDS             5626..6159
                     /gene="blc"
                     /locus_tag="ACHUN9_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0697840.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6156..6473)
                     /gene="sugE"
                     /locus_tag="ACHUN9_004686"
     CDS             complement(6156..6473)
                     /gene="sugE"
                     /locus_tag="ACHUN9_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0697841.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(6648..6794)
                     /gene="ecnB"
                     /locus_tag="ACHUN9_004687"
     CDS             complement(6648..6794)
                     /gene="ecnB"
                     /locus_tag="ACHUN9_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0697842.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(6905..7030)
                     /gene="ecnA"
                     /locus_tag="ACHUN9_004688"
     CDS             complement(6905..7030)
                     /gene="ecnA"
                     /locus_tag="ACHUN9_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0697843.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7082..7648)
                     /gene="efp"
                     /locus_tag="ACHUN9_004689"
     CDS             complement(7082..7648)
                     /gene="efp"
                     /locus_tag="ACHUN9_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0697844.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            7690..8718
                     /gene="epmB"
                     /locus_tag="ACHUN9_004690"
     CDS             7690..8718
                     /gene="epmB"
                     /locus_tag="ACHUN9_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0697845.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2230 a         2305 c         2332 g         2088 t
ORIGIN      
        1 gcgttcagcg acccgatgat cttcggtact gatttgcgcc agtgatgcct ggctggctgg
       61 aaagtggtac acaaaggtcg gtttttcttt gccaatattt ggctctacgc caaaggtaaa
      121 cagcaattgt agcaacgtgt cgcggtcttc ttcggtatca gcaacgttac tcaaatccag
      181 tttcgctgcg acttcccgca gttgcgtttt gtcggcagag agcgggtcaa tttccagata
      241 acgcaagaaa gcttgttgat aagaaaggct ttctgctgcc gggcagtcca gcacctgttg
      301 taagagatcg tccacctcgt tcatcaaccg gtacatatca tagtgcggtc gataccactc
      361 aagcatagtg aactcagggt tgtgataacg ccccatctct tcattacgga agctgcggca
      421 cagctggaat accggcccac aaccggcaac cagcaggcgt ttcatatggt attccgggct
      481 ggtcattaac cagagattca tcccctgcga atgcccgggg ccaacgaaac gtgtctcaaa
      541 cgggaccaaa tgaatatcgg ttaccgtcgc ctggctcata caaggcgtct ccacctccag
      601 cactccacga tcggcaaaga aacgacggat ctccgccata atcgccgcac gttttaataa
      661 gttaggaatg gatgcgctcg gctgccagga tgccgtttcg ctcatagtta aatctccagt
      721 ttttgacaag ggcacgaagt ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa
      781 tcaaacagcg gtgggcagtg actaaaaaaa gcacgatctg atggtttagt aattaaatta
      841 atcatcttca gtgataattt agccctcttg cgcactaaaa aaatcgatct cgtcaaattt
      901 cagacttatc catcagacta tactgttgta cctataaagg agcagtggaa tagcgttcgc
      961 agaccgtaac tttcaggcac ttaccctgaa gtacggggct gtgggataaa aacaatctgg
     1021 aggaatgtcg tgcaaacctt tcaagccgat cttgccattg taggcgccgg tggcgcggga
     1081 ttacgtgctg caattgctgc cgcgcaggca aatccaaatg caaaaatcgc actaatctca
     1141 aaagtatacc cgatgcgtag ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg
     1201 caggatcatg acagcttcga atatcacttt cacgatacag tagcgggtgg cgactggttg
     1261 tgtgagcagg atgtcgtgga ttatttcgtc caccactgcc caaccgaaat gacccaactg
     1321 gaactgtggg ggtgcccatg gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc
     1381 ggcggcatga aaatcgagcg cacctggttc gccgccgata agaccggctt ccatatgctg
     1441 cacacgctgt tccagacctc tctgcaattc ccgcagatcc agcgttttga cgaacatttc
     1501 gtgctggata ttctggttga tgatggtcat gttcgcggcc tggtagcaat gaacatgatg
     1561 gaaggcacgc tggtgcagat ccgtgctaac gcggtcgtta tggctactgg cggtgcgggt
     1621 cgcgtttatc gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg
     1681 ctaagccacg gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg
     1741 ccaggttccg gtatcctgat gaccgaaggc tgccgtggtg aaggcggtat tctggtcaac
     1801 aaaaatggct accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag
     1861 ccgaaaaaca aatatatgga actgggtcca cgcgacaaag tttctcaggc cttctggcac
     1921 gaatggcgta aaggcaacac catctccacg ccgcgtggcg atgtggttta tctcgacttg
     1981 cgtcacctcg gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa
     2041 gcgtacgttg gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac cgcacactac
     2101 accatgggcg gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc
     2161 gtgggtgaat gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc caactccctg
     2221 gcggaactgg tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact
     2281 gccggtaatg gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg
     2341 aaagatctgg ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc
     2401 ctggcaatgg aagaaggttg cggtatctac cgtacgccgg aactgatgca gaaaaccatc
     2461 gacaagctgg cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc
     2521 gtgttcaaca ccgacctgct ctacaccatt gaactgggcc acggtctgaa cgttgctgaa
     2581 tgtatggcgc actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac
     2641 gaaggttgca ccgagcgtga cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat
     2701 gctgatggca cgactcgcct agagtacagc gacgtgaaga ttactacgct gccgccagct
     2761 aaacgcgttt acggtggcga agcggatgca gccgataagg cggaagcagc caataagaag
     2821 gagaaggcga atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga
     2881 agtcgatacc gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt
     2941 actggatgcg ctgggctaca tcaaagacaa cctggcaccg gacctgagtt accgctggtc
     3001 ctgccgtatg gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc
     3061 atgtaaaacc ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt
     3121 cccgattgaa cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat
     3181 caaaccgtac atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc
     3241 ggcgcagatg gcaaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc
     3301 cgcgtgcccg cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc
     3361 gcatcgttat aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa
     3421 cagccagaac ggcgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa
     3481 acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct
     3541 tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg
     3601 tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc
     3661 gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc gggctgtttg
     3721 ccctgaaaaa tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta
     3781 tcgtgatcat taacctgatc actctggcgg cagctctgct gcacaccaaa acctggtttg
     3841 aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa
     3901 ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc
     3961 tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta
     4021 ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc
     4081 ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag
     4141 cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt
     4201 ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaattcac
     4261 gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg
     4321 attggtatcg ttacaatcta acgtatcgcc aatgtaaatc cggcccgcct atggcgggcc
     4381 gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg
     4441 cctcttgctc cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta
     4501 ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta
     4561 aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc
     4621 aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgacg
     4681 ctattgctcg aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta
     4741 ctgctaaaca gtggaatggg atcacactat tacatcttgc gacctacacc gctggcggcc
     4801 tgccattgca ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa
     4861 actggcagcc tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt
     4921 tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc
     4981 gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtacctccc ccagaagaaa
     5041 agaattacgc ctggggatat cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag
     5101 atgctgaagc ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtgcaaagca
     5161 atttaacccc ccttgatatc aatgagaaaa cgcttcaaca agggatacaa ctggcacaat
     5221 ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc
     5281 cggtcaatcc tgacagcatc attaacggca gtgacaataa aattgcactg gcagcacgcc
     5341 cggtaaaagc gattaagccc ccaactcctg cagtacgcgc atcatgggta cataaaacgg
     5401 gggcgaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatcg
     5461 tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tgtcagattc
     5521 tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc
     5581 cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct
     5641 ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gcagttctcc tacgccgccg
     5701 cgtggcgtga cagtagtgaa taatttcgac gctaaacgct atctcggtac ctggtatgag
     5761 attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagt
     5821 ctgcgtgatg acggcggcct gaatgtcatc aataaaggct ataaccctga cagagaaatg
     5881 tggcagcaga gtgaagggaa agcgtacttt accggcgcac caacacgcgc tgcgctgaaa
     5941 gtgtcattct ttggtccttt ctatggcggt tataacgtca ttgcacttga tcgggaatac
     6001 cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca
     6061 accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga agggtttgat
     6121 gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag tttcagacca
     6181 ataatcccca atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcaccg
     6241 agcagcacaa tgccggtgat ggccgcgccg actgcgccaa tacccgtcca cacggcataa
     6301 gccgtcccta ccggtaacga tttcatcgcc caggcaagta gcgccatact gactatcatc
     6361 gccgtcacag taataacact tggcgtcaaa cgactaaagc cgtgggtata tttcaggcca
     6421 acggcccata ccacttccag cagaccagca ataactaaga taatccagga catatcaggc
     6481 tccggagaat tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc ctgcaaagct
     6541 gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac ctttgtgact
     6601 atcgcaaaaa aacaaacggc acgacataac tgtcgtaccg ttattgctta ttgctgcgct
     6661 ttcgttgctg caccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc
     6721 gtggtgttgc aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt
     6781 gtcttcttca ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa
     6841 ttaataatag acaacatcgc caaaaatgat ggaaatctga tgatttttcg gaagagaaga
     6901 aaaattagct ggcagcgcgg gagatggagt tgccgagatg tttgatgtct tcgccgaaac
     6961 cgcgagcggt gttacagccc gtgagcagcg tgctggcaag caaaacaaga acgataaggc
     7021 gtttcatcat ctctgacctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc
     7081 attacttcac gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga
     7141 tttgtacgaa cagcggaact ttaaccacag cgccagtaga cagggtagcc ggtttgccgc
     7201 cagtacctgc ggtatcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga
     7261 agttcggcgg agtaacggag atcggctgac cattccacag agttacgata cactctgcct
     7321 gatccagcag ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac agctgctcga
     7381 aagtttcgtt gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca
     7441 tatcgacaac atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag
     7501 taccggtcag cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta
     7561 cgaattcact cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac
     7621 gaaaatcgtt gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta
     7681 agccacaaaa tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg
     7741 caacttgccg atgttgtgac cgatcctggt gaacttctgc gtcttttgaa tatagacgcg
     7801 gacgaaaaac tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc
     7861 tcatttatcg atcgcatgga gaaaggcaat ccggacgatc ctattttgcg tcaggtactt
     7921 acctcgcaag atgagtttgt cgtcgcgtcc ggattctcca ccgacccact ggaagaacag
     7981 cacagcgtag tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa
     8041 ggcggctgcg cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat
     8101 cagggcaaca agcgtaactg gcagactgca cttgagtatg ttgctgcgca tccggaactg
     8161 gacgagatga ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg
     8221 ttactcacac aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg
     8281 ccgattgtga tcccggcacg tatcaccgag gcgctggttg aacgctttgc ccgttctacg
     8341 ctgcaaatct tgctggtgaa tcacatcaat catgccaatg aggtagatga aacgttccgt
     8401 caggcgatgg ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta
     8461 cgtggtgtga acgataacgc acaaacgttg gcaaacctga gtaatgcgtt gttcgatgcc
     8521 ggcgtgatgc cgtattacct gcatgtgcta gataaagtac agggcgcggc gcattttatg
     8581 gtgagtgatg acgaagcacg gcagattatg cgtgagttgc tgacattggt gtcgggatat
     8641 ctggtgccga aactggcgcg agaaattggc ggcgaaccca gcaaaacgcc gctggatctc
     8701 cagctacgcc agcagtaact gattaaacag ggaagttaaa cattagcctg atgcgctacg
     8761 cttatcaggc ctacattgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga
     8821 taaggcgttt acgccgcatc cggcaagagc aacaaacgca agtacagcaa cctgtcatta
     8881 aactgggaaa tttaatcttt gcccgttgca taacctttaa ttattcatat acattgctgt
     8941 tgttactctt tatat
//
LOCUS       DAZOYS010000591         5364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2206296
            SAMN43277660-rid23486513.guided.235, whole genome shotgun sequence.
ACCESSION   DAZOYS010000591 DAZOYS010000000
VERSION     DAZOYS010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277660
            Sequence Read Archive: SRR30464458
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396809.1
            Genome Coverage       :: 107x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 07:50:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,696
            CDSs (total)                      :: 4,606
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 90
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 128 of 285
            Pseudo Genes (incomplete)         :: 107 of 285
            Pseudo Genes (internal stop)      :: 93 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277660-rid23486513.guided.235"
                     /strain="M2206296"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2022-08-23"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUN9_004691"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUN9_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUN9_004692"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUN9_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0697846.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUN9_004693"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUN9_004693"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0697847.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUN9_004694"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUN9_004694"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0697848.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUN9_004695"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUN9_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0697849.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHUN9_004696"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHUN9_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0697850.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
BASE COUNT         1299 a         1315 c         1437 g         1313 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgctt ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atat
//
